title: An ancestral allele of grapevine transcription factor MYB14 promotes plant defence.
abstract: Stilbene synthase is a key enzyme for the production of the phytoalexin resveratrol. Some clones of Vitis sylvestris, a wild European grapevine species which is almost extinct, have been shown to accumulate more resveratrol in response to different forms of stress. In the current study, we asked whether the induction of stilbene synthase transcripts in Hoe29, one of the V. sylvestris clones with elevated stilbene inducibility, might result from the elevated induction of the transcription factor MYB14. The MYB14 promoter of Hoe29 and of Ke83 (a second stilbene-inducible genotype) harboured distinct regions and were applied to a promoter-reporter system. We show that stilbene synthase inducibility correlates with differences in the induction of MYB14 transcripts for these two genotypes. Both alleles were induced by UV in a promoter-reporter assay, but only the MYB14 promoter from Hoe29 was induced by flg22, consistent with the stilbene synthase expression of the donor genotypes, where both respond to UV but only Hoe29 is responsive to Plasmopara viticola during defence. We mapped upstream signals and found that a RboH-dependent oxidative burst, calcium influx, a MAPK cascade, and jasmonate activated the MYB14 promoter, whereas salicylic acid was ineffective. Our data suggest that the Hoe29 allele of the MYB14 promoter has potential as a candidate target for resistance breeding.
authors: Molecular Cell Biology, Botanical Institute 1, Karlsruhe Institute of Technology, Kaiserstr. 2, D-76131 Karlsruhe, Germany College of Life Sciences, Northwest University, Xi'an 710069, China ddong.sh@hotmail.com.
authors: Dong
authors: D
authors: Duan
authors: Institute of Molecular Genetics, Johannes Gutenberg-University Mainz, J.-J.-Becherweg 32, D-55128 Mainz, Germany.
authors: Sabine
authors: S
authors: Fischer
authors: Dienstleistungszentrum Ländlicher Raum Rheinpfalz, Breitenweg 71, Viticulture and Enology Group, D-67435 Neustadt, Germany.
authors: Patrick
authors: P
authors: Merz
authors: Dienstleistungszentrum Ländlicher Raum Rheinpfalz, Breitenweg 71, Viticulture and Enology Group, D-67435 Neustadt, Germany Fachhochschule Bingen, D-55411 Bingen am Rhein, Germany.
authors: Jochen
authors: J
authors: Bogs
authors: Molecular Cell Biology, Botanical Institute 1, Karlsruhe Institute of Technology, Kaiserstr. 2, D-76131 Karlsruhe, Germany.
authors: Michael
authors: M
authors: Riemann
authors: Molecular Cell Biology, Botanical Institute 1, Karlsruhe Institute of Technology, Kaiserstr. 2, D-76131 Karlsruhe, Germany.
authors: Peter
authors: P
authors: Nick
mesh_terms: D000483:Alleles; D005838:Genotype; D008954:Models, Biological; D009861:Onium Compounds / Q000494:pharmacology; D009868:Oomycetes / Q000502:physiology / Q000528:radiation effects; D057865:Plant Immunity / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays; D027843:Vitis / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology / Q000528:radiation effects
chemical_list: D009861:Onium Compounds; D010940:Plant Proteins; D014157:Transcription Factors; C007517:diphenyleneiodonium
keywords: MYB14; Plasmopara viticola; UV.; flg22; grapevine (V. sylvestris); stilbene synthase