title: Early assessment of a rapid alternative method for the estimation of the biomethane potential of sewage sludge.
abstract: This short communication briefly presents a rapid method using a fluorescent redox indicator, similar to resazurin, in order to estimate the biodegradability of sewage sludge during anaerobic digestion (AD). The biodegradability and by extension the Biochemical Methane Potential (BMP) of nineteen municipal sludge samples (primary, biological and tertiary) were investigated and estimated in only 48 h. Results showed the relevance to follow the metabolic activity of anaerobic sludge by the kinetic of probe reduction. The extended lag phase of inoculum indicated an impact of pre-treatments on enzyme activity. The comparison with Automatic Methane Potential Test System II (AMPTS) confirmed the estimated values of BMP according to an uncertainty limit of 25%. These first results highlight the interest of this rapid assay as a preliminary tool of the biodegradability of sewage sludge in anaerobic digestion.
authors: AMS Envolure, 1682 rue de la Valsière, 34184 Montpellier Cedex 4, France. Electronic address: solenn.bellaton@envolure.com.
authors: Solenn
authors: S
authors: Bellaton
authors: SIAAP, Direction du Développement et de la Prospective, 82 Avenue Kléber, 92700 Colombes, France.
authors: Sabrina
authors: S
authors: Guérin
authors: AMS Envolure, 1682 rue de la Valsière, 34184 Montpellier Cedex 4, France; SCANAE, 1682 rue de la Valsière, 34790 Grabels, France.
authors: Nathalie
authors: N
authors: Pautremat
authors: SIAAP, Direction du Développement et de la Prospective, 82 Avenue Kléber, 92700 Colombes, France.
authors: Jean
authors: J
authors: Bernier
authors: AMS Envolure, 1682 rue de la Valsière, 34184 Montpellier Cedex 4, France.
authors: Mathieu
authors: M
authors: Muller
authors: Centre de Recherche Royallieu, Université Technologique de Compiègne, BP 20529, rue Personne de Roberval, 60205 Compiègne Cedex, France.
authors: Stéphane
authors: S
authors: Motellet
authors: SIAAP, Direction du Développement et de la Prospective, 82 Avenue Kléber, 92700 Colombes, France.
authors: Sam
authors: S
authors: Azimi
authors: Centre de Recherche Royallieu, Université Technologique de Compiègne, BP 20529, rue Personne de Roberval, 60205 Compiègne Cedex, France.
authors: André
authors: A
authors: Pauss
authors: SIAAP, Direction du Développement et de la Prospective, 82 Avenue Kléber, 92700 Colombes, France.
authors: Vincent
authors: V
authors: Rocher
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005453:Fluorescence; D007700:Kinetics; D008697:Methane / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry*
chemical_list: D012722:Sewage; D008697:Methane
keywords: Anaerobic digestion; Automatic Methane Potential Test System; Biodegradability; Biomethane production; Fluorescent redox indicator