title: Therapeutic effect of anti-feline TNF-alpha monoclonal antibody for feline infectious peritonitis.
abstract: Feline infectious peritonitis virus (FIPV) replication in macrophages/monocytes induced tumor necrosis factor (TNF)-alpha production, and that the TNF-alpha produced was involved in aggravating the pathology of FIP. We previously reported the preparation of a feline TNF-alpha (fTNF-alpha)-neutralizing mouse monoclonal antibody (anti-fTNF-alpha mAb). This anti-fTNF-alpha mAb 2-4 was confirmed to inhibit the following fTNF-alpha-induced conditions in vitro. In the present study, we investigated whether mAb 2-4 improved the FIP symptoms and survival rate of experimentally FIPV-inoculated SPF cats. Progression to FIP was prevented in 2 out of 3 cats treated with mAb 2-4, whereas all 3 cats developed FIP in the placebo control group. Plasma alpha1-glycoprotein and vascular endothelial growth factor levels were improved by the administration of mAb 2-4, and the peripheral lymphocyte count also recovered. These results strongly suggested that the anti-fTNF-alpha antibody is effective for the treatment of FIP.
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: dv12003f@st.kitasato-u.ac.jp.
authors: Tomoyoshi
authors: T
authors: Doki
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: takanot@vmas.kitasato-u.ac.jp.
authors: Tomomi
authors: T
authors: Takano
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: tachikoma893@yahoo.co.jp.
authors: Kohei
authors: K
authors: Kawagoe
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: kitasato8852@yahoo.co.jp.
authors: Akihiko
authors: A
authors: Kito
authors: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. Electronic address: hohdatsu@vmas.kitasato-u.ac.jp.
authors: Tsutomu
authors: T
authors: Hohdatsu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002415:Cats; D016765:Coronavirus, Feline / Q000276:immunology*; D016766:Feline Infectious Peritonitis / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use*
chemical_list: D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha
keywords: Feline coronavirus; Feline infectious peritonitis; Tumor necrosis factor-alpha