title: A Critical Role of miR-144 in Diffuse Large B-cell Lymphoma Proliferation and Invasion.
abstract: MicroRNAs are endogenous noncoding RNAs that play important roles in a wide variety of biologic processes such as apoptosis, development, aging, and tumorigenesis. The B-cell lymphoma 6 (BCL6) transcriptional repressor has emerged as a critical therapeutic target in diffuse large B-cell lymphomas (DLBCL), but the mechanisms regulating BCL6 are still unclear. In the current study, we screened the microRNA expression profiles in DLBCL specimens and cell lines by qRT-PCR and found that the expression of miR-144 was significantly downregulated in DLBCL tissues and cell lines and negatively correlated with BCL6 expression. We further demonstrated that BCL6 was the direct target gene of miR-144, and miR-144 suppressed the expression of BCL6 via binding the 3'untranslated region of BCL6 mRNA. Biologically, forced expression of miR-144 significantly attenuated cell proliferation and invasion of OCI-Ly3 cells in vitro, and the tumor-suppressor effect of miR-144 was also confirmed using a xenograft mouse model in vivo Taken together, our results reveal that miR-144 regulates BCL6 in DLBCL and provide a rationale for developing strategies that target miR-144 as a therapeutic intervention for DLBCL.
authors: Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China.
authors: Haiying
authors: H
authors: Wang
authors: Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China.
authors: Aihong
authors: A
authors: Wang
authors: Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China.
authors: Zhenbo
authors: Z
authors: Hu
authors: Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China.
authors: Xin
authors: X
authors: Xu
authors: Department of Lymphoma and Myeloma, The University of Texas MD Anderson Cancer Center, Houston, Texas.
authors: Zhiqiang
authors: Z
authors: Liu
authors: Department of Hematology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong, China. zhanju_w@126.com.
authors: Zhanju
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004195:Disease Models, Animal; D015870:Gene Expression; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D051560:Proto-Oncogene Proteins c-bcl-6; D012333:RNA, Messenger