title: Impact of CYP3A5 and MDR-1 gene polymorphisms on the dose and level of tacrolimus among living-donor liver transplanted patients: single center experience.
abstract: OBJECTIVE
  To assess the impact of Cytochrome P450 3A5 (CYP3A5) and multidrug resistance-1 gene (MDR-1) single nucleotide polymorphisms on the dose and blood level of tacrolimus among liver transplanted patients.
  
  
  METHODS
  We enrolled a prospective study of 41 liver transplanted patients. Dose-adjusted trough blood concentration (C/D ratio) was calculated. Polymerase chain reaction-restriction fragment length polymorphism followed by sequencing was done for genotyping of CYP3A5*3 (6986A > G).
  
  
  RESULTS
  At 1 week, 1 and 3 months C/D ratio were significantly lower in CYP3A5 expressers *1/*1 patients compared to non-expressers *3/*3.
  
  
  CONCLUSIONS
  CYP3A5 (6986A > G) genotype, rather than MDR-1 (2677G > A/T) variant, has an impact on tacrolimus pharmacokinetics.
authors: a Clinical and Chemical Pathology Department, Cairo University , Cairo , Egypt and.
authors: Mona
authors: M
authors: Fathy
authors: a Clinical and Chemical Pathology Department, Cairo University , Cairo , Egypt and.
authors: Manal
authors: M
authors: Kamal
authors: a Clinical and Chemical Pathology Department, Cairo University , Cairo , Egypt and.
authors: Abeer
authors: A
authors: Mohy
authors: b Tropical Medicine Department, Faculty of Medicine, Cairo University , Cairo , Egypt.
authors: Ahmad
authors: A
authors: Nabil
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics; D051544:Cytochrome P-450 CYP3A / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D016031:Liver Transplantation; D019520:Living Donors; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016559:Tacrolimus / Q000627:therapeutic use*
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D007166:Immunosuppressive Agents; D051544:Cytochrome P-450 CYP3A; D016559:Tacrolimus
keywords: Cytochrome P450; liver transplantation; multidrug resistance-1; tacrolimus