title: Laminin receptor is an interacting partner for viral outer capsid protein VP5 in grass carp reovirus infection.
abstract: Grass carp reovirus (GCRV) is responsible for viral hemorrhagic disease in cultured grass carp Ctenopharyngon idellus. Through yeast two-hybrid screen, laminin receptor (LamR) was identified as a potential interacting partner for the outer capsid protein VP5 of GCRV. We cloned and sequenced the gene encoding grass carp LamR. Viral attachment assay demonstrated the involvement of membrane-associated LamR in GCRV infection. Solid-phase overlay assays demonstrated that GCRV interacted with GST-tagged LamR in vitro. In contrast to VP7, GST-tagged VP5 was shown to associate with LamR in both pull-down and solid-phase blot overlay assays. With the reduction of LamR expression in CIK cells achieved by RNAi, remarkably reduced infection efficiency of GCRV was observed. CIK cells pretreated with polyclonal antibody against LamR resulted in dose-dependent inhibition of GCRV infection. These results collectively indicated that grass carp LamR was involved in GCRV infection by interacting with viral outer capsid protein VP5.
authors: National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Freshwater Fishery Germplasm Resources, Shanghai Ocean University, 201306 Shanghai, PR China.
authors: Hao
authors: H
authors: Wang
authors: National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Freshwater Fishery Germplasm Resources, Shanghai Ocean University, 201306 Shanghai, PR China.
authors: Fei
authors: F
authors: Yu
authors: National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Freshwater Fishery Germplasm Resources, Shanghai Ocean University, 201306 Shanghai, PR China.
authors: Jiale
authors: J
authors: Li
authors: National Pathogen Collection Center for Aquatic Animals, Key Laboratory of Freshwater Fishery Germplasm Resources, Shanghai Ocean University, 201306 Shanghai, PR China. Electronic address: lqlv@shou.edu.cn.
authors: Liqun
authors: L
authors: Lu
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D002347:Carps; D005393:Fish Diseases / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012087:Reoviridae / Q000235:genetics / Q000378:metabolism*; D012088:Reoviridae Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology
chemical_list: D036022:Capsid Proteins; D029941:Fish Proteins; D017474:Receptors, Laminin; D011991:Receptors, Virus
keywords: Grass carp reovirus; Infection inhibition; Laminin receptor; Outer capsid; Protein–protein interaction