title: Effect of surface potential on epithelial cell adhesion, proliferation and morphology.
abstract: Cell adhesion is the basis of individual cell survival, division and motility. Hence, understanding the effects that the surface properties have on cell adhesion, proliferation and morphology are crucial. In particular, surface charge/potential has been identified as an important factor that affects cell behavior. However, how cells respond to incremental changes in surface potential remains unclear. By using binary self-assembled monolayer (SAM) modified Au surfaces that are similar in mechanical/chemical properties and provide a series of surface potentials, the effect of surface potential on the behavior of cells can be studied. In this work, the effect of surface potential on epithelial cells, including human embryonic kidney (HEK293T) and human hepatocellular carcinoma (HepG2), were examined. The results showed that the adhesion density of epithelial cells increased with increasing surface potential, which is similar to but varied more significantly compared with fibroblasts. The proliferation rate is found to be independent of surface potential in both cell types. Furthermore, epithelial cells show no morphological change with respect to surface potential, whereas the morphology of the fibroblasts clearly changed with the surface potential. These differences between the cell types were rationalized by considering the difference in extracellular matrix composition. Laminin-dominant epithelial cells showed higher adhesion density and less morphological change than did fibronectin-dominant fibroblasts because the more significant adsorption of positively charged laminin on the surface enhanced the adhesion of epithelial cells. In contrast, due to the dominance of negatively charged fibronectin that adsorbed weakly on the surface, fibroblasts had to change their morphology to fit the inhomogeneous fibronectin-adsorbed area.
authors: Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan; Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica and National Tsing Hua University, Taiwan; Institude of Physics, Academia Sinica, Taipei, Taiwan.
authors: Hsun-Yun
authors: HY
authors: Chang
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan.
authors: Wei-Lun
authors: WL
authors: Kao
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
authors: Yun-Wen
authors: YW
authors: You
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan.
authors: Yi-Hsuan
authors: YH
authors: Chu
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan.
authors: Kuo-Jui
authors: KJ
authors: Chu
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
authors: Peng-Jen
authors: PJ
authors: Chen
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan.
authors: Chen-Yi
authors: CY
authors: Wu
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
authors: Yu-Hsuan
authors: YH
authors: Lee
authors: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan; Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan. Electronic address: shyue@gate.sinica.edu.tw.
authors: Jing-Jong
authors: JJ
authors: Shyue
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D048430:Cell Shape / Q000502:physiology*; D004847:Epithelial Cells / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D007797:Laminin / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D005353:Fibronectins; D007797:Laminin; D006046:Gold
keywords: Cell adhesion; Epithelial cell; Self-assembled monolayer (SAM); Surface potential