title: Characterizing 3D RNA structure by single molecule FRET.
abstract: The importance of elucidating the three dimensional structures of RNA molecules is becoming increasingly clear. However, traditional protein structural techniques such as NMR and X-ray crystallography have several important drawbacks when probing long RNA molecules. Single molecule Förster resonance energy transfer (smFRET) has emerged as a useful alternative as it allows native sequences to be probed in physiological conditions and allows multiple conformations to be probed simultaneously. This review serves to describe the method of generating a three dimensional RNA structure from smFRET data from the biochemical probing of the secondary structure to the computational refinement of the final model.
authors: NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors: James D
authors: JD
authors: Stephenson
authors: Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Hills Rd, Cambridge CB2 0QQ, UK.
authors: Julia C
authors: JC
authors: Kenyon
authors: Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
authors: Martyn F
authors: MF
authors: Symmons
authors: Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Hills Rd, Cambridge CB2 0QQ, UK. Electronic address: amll1@medschl.cam.ac.uk.
authors: Andrew M L
authors: AM
authors: Lever
mesh_terms: D001483:Base Sequence; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000648:ultrastructure; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D012313:RNA
keywords: 3D model; FRET; Fluorescence; RNA structure; Single-molecule