title: Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases.
abstract: The goal of this study was to define Na,K-ATPase α and β subunit isoform expression and isozyme composition in colorectal cancer cells and liver metastases. The α1, α3, and β1 isoforms were the most highly expressed in tumor cells and metastases; in the plasma membrane of non-neoplastic cells and mainly in a cytoplasmic location in tumor cells. α1β1 and α3β1 isozymes found in tumor and metastatic cells exhibit the highest and lowest Na(+) affinity respectively and the highest K(+) affinity. Mesenchymal cell isozymes possess an intermediate Na(+) affinity and a low K(+) affinity. In cancer, these ions are likely to favor optimal conditions for the function of nuclear enzymes involved in mitosis, especially a high intra-nuclear K(+) concentration. A major and striking finding of this study was that in liver, metastasized CRC cells express the α3β1 isozyme. Thus, the α3β1 isozyme could potentially serve as a novel exploratory biomarker of CRC metastatic cells in liver.
authors: Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias, Universidad de La Laguna Santa Cruz de Tenerife, Spain.
authors: Marc
authors: M
authors: Baker Bechmann
authors: Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias, Universidad de La LagunaSanta Cruz de Tenerife, Spain; Institute of Endocrinology and Experimental Oncology, National Research CouncilNaples, Italy.
authors: Deborah
authors: D
authors: Rotoli
authors: Service of Medical Oncology, University Hospital Nuestra Señora de CandelariaSanta Cruz de Tenerife, Spain; Medical Oncology, Hospiten HospitalsSanta Cruz de Tenerife, Spain.
authors: Manuel
authors: M
authors: Morales
authors: Service of Pathology, University Hospital Nuestra Señora de Candelaria Santa Cruz de Tenerife, Spain.
authors: María Del Carmen
authors: Mdel C
authors: Maeso
authors: Department of Pathology, Hospiten Hospitals Santa Cruz de Tenerife, Spain.
authors: María Del Pino
authors: Mdel P
authors: García
authors: Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias, Universidad de La Laguna Santa Cruz de Tenerife, Spain.
authors: Julio
authors: J
authors: Ávila
authors: Department of Veterinary Preclinical Sciences, Faculty of Health and Medical Sciences, University of SurreyGuildford, UK; Faculty of Applied Medical Sciences, Center of Excellence in Genomic Medicine Research, King Fahd Medical Research Center, King AbdulAziz UniversityJeddah, Saudi Arabia.
authors: Ali
authors: A
authors: Mobasheri
authors: Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias, Universidad de La Laguna Santa Cruz de Tenerife, Spain.
authors: Pablo
authors: P
authors: Martín-Vasallo
keywords: Na/K-ATPase isoforms colorectal cancer immunohistochemistry; Na/K-ATPase isozymes; colorectal cancer; colorectal cancer liver metastases; sodium pump isozymes