title: Immune-Modulating Activity of Extract Prepared from Mycelial Culture of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes).
abstract: Ophiocordyceps sinensis is a natural fungus that has been valued as a health food and traditional Chinese medicine for centuries. The fungus is parasitic and colonizes insect larva. Naturally occurring O. sinensis thrives at high altitude in cold and grassy alpine meadows on the Himalayan mountain ranges. Wild O. sinensis is becoming increasingly rare in its natural habitats, and its price is out of reach for clinical practice. For these reasons, development of a standardized alternative is a great focus of research to allow the use of O. sinensis as a medicine. To develop an alternative for wild O. sinensis, a refined standardized extract, CBG-CS-2, was produced by artificial fermentation and extraction of the mycelial strain Paecilomyces hepiali CBG-CS-1, which originated from wild O. sinensis. In this study, we analyzed the in vivo immune-modulating effect of CBG-CS-2 in mice. Oral administration of CBG-CS-2 supported splenocyte stimulation and enhanced Th1-type cytokine expression from the splenocytes. Importantly, the same treatment significantly enhanced the natural killer cell activity of the splenocytes. Finally, oral administration of CBG-CS-2 enhanced the potential for inflammatory responses. Together, these findings indicate that the mycelial culture extract prepared from O. sinensis exhibited immune-modulating activity and suggest its possible use in the treatment of diseases caused by abnormal immune function.
authors: Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Korea.
authors: Sun-Hee
authors: SH
authors: Jang
authors: Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Korea; Department of Bioactive Materials and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Korea.
authors: Sae-Hae
authors: SH
authors: Kim
authors: Department of Bioactive Materials and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Korea.
authors: Ha-Yan
authors: HY
authors: Lee
authors: Chebigen Inc., Jeonju, Korea.
authors: Seung-Hwan
authors: SH
authors: Jang
authors: Department of Oral and Maxillofacial Surgery, Korea University Ansan Hospital, Ansan, Korea.
authors: Hyonseok
authors: H
authors: Jang
authors: Department of Pharmacology, Chonbuk National University Medical School, Jeonju, Korea; Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju, Korea.
authors: Soo-Wan
authors: SW
authors: Chae
authors: Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju, Korea.
authors: Su-Jin
authors: SJ
authors: Jung
authors: Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju, Korea.
authors: Byung-Ok
authors: BO
authors: So
authors: Healthcare Claims & Management Inc., Jeonju, Korea.
authors: Ki-Chan
authors: KC
authors: Ha
authors: Chebigen Inc., Jeonju, Korea.
authors: Hong-Sig
authors: HS
authors: Sin
authors: Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Korea; Department of Bioactive Materials and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Korea.
authors: Yong-Suk
authors: YS
authors: Jang
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D001688:Biological Products / Q000302:isolation & purification* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D006999:Hypocreales / Q000737:chemistry; D007155:Immunologic Factors / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D025282:Mycelium / Q000254:growth & development / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D013154:Spleen / Q000166:cytology / Q000187:drug effects
chemical_list: D001688:Biological Products; C000611835:CBG-CS-2; D016207:Cytokines; D007155:Immunologic Factors; D004364:Pharmaceutical Preparations