title: Integrating ribosomal promoter vectors that offer a choice of constitutive expression profiles in Leishmania donovani.
abstract: We have designed a novel series of integrating ribosomal RNA promoter vectors with five incrementally different constitutive expression profiles, covering a 250-fold range. Differential expression was achieved by placing different combinations of synthetic or leishmanial DNA sequences upstream and downstream of the transgene coding sequence in order to modulate pre-mRNA processing efficiency and mRNA stability, respectively. All of the vectors have extensive multiple cloning sites, and versions are available for producing N- or C- terminal GFP fusions at each of the possible relative expression levels. In addition, the modular configuration of the vectors allows drug resistance cassettes and other components to be readily exchanged. In toto, these vectors should be useful additions to the toolkit available for molecular and genetic studies of Leishmania donovani.
authors: Department of Biochemistry and Molecular Biology, Oregon Health & Science University, Portland, OR 97239, USA.
authors: Radika
authors: R
authors: Soysa
authors: Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, OR 97239, USA.
authors: Khoa D
authors: KD
authors: Tran
authors: Department of Biochemistry and Molecular Biology, Oregon Health & Science University, Portland, OR 97239, USA.
authors: Buddy
authors: B
authors: Ullman
authors: Department of Biochemistry and Molecular Biology, Oregon Health & Science University, Portland, OR 97239, USA. Electronic address: yatesp@ohsu.edu.
authors: Phillip A
authors: PA
authors: Yates
mesh_terms: D020869:Gene Expression Profiling; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007893:Leishmania donovani / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes
chemical_list: D049452:Green Fluorescent Proteins
keywords: Expression vector; Green fluorescent protein; Leishmania donovani; Ribosomal RNA promoter