title: Gastroprotective Effects of Astragaloside IV against Acute Gastric Lesion in Rats.
abstract: BACKGROUND
  Protection of the gastric mucosa from acute lesions induced by various irritants is a pertinent issue in the field of critical care medicine. In this study, we investigated the gastroprotective effects of astragaloside IV on acute gastric lesions in rats under stressful conditions.
  
  
  METHODS
  Rats were randomized into six groups. Group 1 and 2 received 10% Tween 80 (vehicle). Group 3 received 20 mg/kg of omeprazole, a proton pump inhibitor. Groups 4, 5 and 6 received astragaloside IV at concentration of 1, 10, and 50 mg/kg, respectively. As a means to induce gastric lesions, Groups 2-6 were subjected to water immersion and restraint stress for 12 hours after treatment.
  
  
  RESULTS
  Our present studies show that compared to rats in group 2, treatment with 1 to 50 mg/kg astragaloside IV significantly decreased the size of gastric lesions, MDA, TNFα and MCP1 levels, in addition to normalizing gastric pH, gastric mucus and SOD levels (P<0.05). Histomorphological examination confirmed that treatment with astragaloside IV elicited a dosage-dependent protective effect on the gastric mucosa. Furthermore, pretreatment with astragaloside IV resulted in significant elevations in HSP70 and reduction in Bax, along with over-expression of PLCγ response level, which was further confirmed via immunohistochemical analysis.
  
  
  CONCLUSIONS
  The acute gastric lesions induced are attenuated by pretreatment with astragaloside IV which is possibly due to the enhancing of the expression of HSP70 with concomitant antioxidant, anti-inflammatory and anti-apoptotic capacity.
authors: Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Road Dade, Guangzhou 510120, China.|Second Clinical Medical College, Guangzhou University of Chinese Medicine, Road Jichang, Guangzhou 510405, China.|Physiology & Experimental Medicine, Hospital for Sick Children, Toronto M5G 0A4, Canada.
authors: Shuai
authors: S
authors: Mao
authors: Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Road Dade, Guangzhou 510120, China.|Second Clinical Medical College, Guangzhou University of Chinese Medicine, Road Jichang, Guangzhou 510405, China.
authors: Guang
authors: G
authors: Yang
authors: Faculty of Medicine, University of Toronto, University Ave., Toronto M5G 0A4, Canada.
authors: Winny
authors: W
authors: Li
authors: Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Road Dade, Guangzhou 510120, China.|Second Clinical Medical College, Guangzhou University of Chinese Medicine, Road Jichang, Guangzhou 510405, China.
authors: Jian
authors: J
authors: Zhang
authors: Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Road Dade, Guangzhou 510120, China.|Second Clinical Medical College, Guangzhou University of Chinese Medicine, Road Jichang, Guangzhou 510405, China.
authors: Hailong
authors: H
authors: Liang
authors: Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Road Dade, Guangzhou 510120, China.|Second Clinical Medical College, Guangzhou University of Chinese Medicine, Road Jichang, Guangzhou 510405, China.
authors: Jian
authors: J
authors: Li
authors: Department of Critical Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Road Dade, Guangzhou 510120, China.|Second Clinical Medical College, Guangzhou University of Chinese Medicine, Road Jichang, Guangzhou 510405, China.
authors: Minzhou
authors: M
authors: Zhang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D018932:Chemokine CCL2 / Q000378:metabolism; D005753:Gastric Mucosa / Q000293:injuries / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009853:Omeprazole / Q000009:adverse effects; D051966:Phospholipase C gamma / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000627:therapeutic use*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; C494828:Bax protein, rat; C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D018840:HSP70 Heat-Shock Proteins; D012503:Saponins; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; C052064:astragaloside A; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D051966:Phospholipase C gamma; D009853:Omeprazole