title: Cellulose conjugated FITC-labelled mesoporous silica nanoparticles: intracellular accumulation and stimuli responsive doxorubicin release.
abstract: Herein, we design novel cellulose conjugated mesoporous silica nanoparticle (CLS-MSP) based nanotherapeutics for stimuli responsive intracellular doxorubicin (DOX) delivery. DOX molecules are entrapped in pores of the fabricated mesoporous silica nanoparticles (MSPs) while cellulose is used as an encapsulating material through esterification on the outlet of the pores of the MSPs to avoid premature DOX release under physiological conditions. In in vitro studies, stimuli responsive DOX release is successfully achieved from DOX loaded cellulose conjugated mesoporous silica nanoparticles (DOX/CLS-MSPs) by pH and cellulase triggers. Intracellular accumulation of DOX/CLS-MSPs in human liver cancer cells (HepG2 cells) is investigated through confocal microscope magnification. Cell viability of HepG2 cells is determined as the percentage of the cells incubated with DOX/CLS-MSPs compared with that of non-incubated cells through an MTT assay.
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn and Lasbela University of Agriculture, Water and Marine Sciences Uthal, Pakistan.
authors: Abdul
authors: A
authors: Hakeem
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn.
authors: Fouzia
authors: F
authors: Zahid
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn.
authors: Ruixue
authors: R
authors: Duan
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn.
authors: Muhammad
authors: M
authors: Asif
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn.
authors: Tianchi
authors: T
authors: Zhang
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn.
authors: Zhenyu
authors: Z
authors: Zhang
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn.
authors: Yong
authors: Y
authors: Cheng
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn.
authors: Xiaoding
authors: X
authors: Lou
authors: School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Louxiaoding@hust.edu.cn xiafan@hust.edu.cn and Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Huazhong University of Science and Technology, Wuhan 430074, China. xiafan@hust.edu.cn and Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Fan
authors: F
authors: Xia
mesh_terms: D002482:Cellulose / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D003692:Delayed-Action Preparations; D012822:Silicon Dioxide; D004317:Doxorubicin; D002482:Cellulose; D016650:Fluorescein-5-isothiocyanate