title: Genome-Wide Identification of QTL for Seed Yield and Yield-Related Traits and Construction of a High-Density Consensus Map for QTL Comparison in Brassica napus.
abstract: Seed yield (SY) is the most important trait in rapeseed, is determined by multiple seed yield-related traits (SYRTs) and is also easily subject to environmental influence. Many quantitative trait loci (QTLs) for SY and SYRTs have been reported in Brassica napus; however, no studies have focused on seven agronomic traits simultaneously affecting SY. Genome-wide QTL analysis for SY and seven SYRTs in eight environments was conducted in a doubled haploid population containing 348 lines. Totally, 18 and 208 QTLs for SY and SYRTs were observed, respectively, and then these QTLs were integrated into 144 consensus QTLs using a meta-analysis. Three major QTLs for SY were observed, including cqSY-C6-2 and cqSY-C6-3 that were expressed stably in winter cultivation area for 3 years and cqSY-A2-2 only expressed in spring rapeseed area. Trait-by-trait meta-analysis revealed that the 144 consensus QTLs were integrated into 72 pleiotropic unique QTLs. Among them, all the unique QTLs affected SY, except for uq.A6-1, including uq.A2-3, uq.C1-2, uq.C1-3, uq.C6-1, uq.C6-5, and uq.C6-6 could also affect more than two SYRTs. According to the constructed high-density consensus map and QTL comparison from literatures, 36 QTLs from five populations were co-localized with QTLs identified in this study. In addition, 13 orthologous genes were observed, including five each gene for SY and thousand seed weight, and one gene each for biomass yield, branch height, and plant height. The genomic information of these QTLs will be valuable in hybrid cultivar breeding and in analyzing QTL expression in different environments.
authors: Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic ImprovementYangling, China; Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China.
authors: Weiguo
authors: W
authors: Zhao
authors: Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors: Xiaodong
authors: X
authors: Wang
authors: Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic ImprovementYangling, China; Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China.
authors: Hao
authors: H
authors: Wang
authors: Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement Yangling, China.
authors: Jianhua
authors: J
authors: Tian
authors: Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement Yangling, China.
authors: Baojun
authors: B
authors: Li
authors: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors: Li
authors: L
authors: Chen
authors: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors: Hongbo
authors: H
authors: Chao
authors: Institute of Biotechnology, Chinese Academy of Agricultural Sciences Beijing, China.
authors: Yan
authors: Y
authors: Long
authors: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University Huanggang, China.
authors: Jun
authors: J
authors: Xiang
authors: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University Huanggang, China.
authors: Jianping
authors: J
authors: Gan
authors: Department of Applied Biological Science, College of Agriculture and Biotechnology, Zhejiang University Hangzhou, China.
authors: Wusheng
authors: W
authors: Liang
authors: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal UniversityHuanggang, China.
authors: Maoteng
authors: M
authors: Li
keywords: Brassica napus; candidate genes; map comparsion; quantitative trait loci; seed yield; seed yield-related traits