title: Functional attributes of evolutionary conserved Arg45 of Wolbachia (Brugia malayi) translation initiation factor-1.
abstract: OBJECTIVE
  Wolbachia is a promising antifilarial chemotherapeutic target. Translation initiation factor-1 (Tl IF-1) is an essential factor in prokaryotes. Functional characterization of Wolbachia's novel proteins/enzymes is necessary for the development of adulticidal drugs.
  
  
  METHODS
  Mutant, Wol Tl IF-1 R45D was constructed by site directed mutagenesis. Fluorimetry and size exclusion chromatography were used to determine the biophysical characteristics. Mobility shift assay and fluorescence resonance energy transfer were used to investigate the functional aspect of Wol Tl IF-1 with its mutant.
  
  
  RESULTS
  Both wild and mutant were in monomeric native conformations. Wild exhibits nonspecific binding with ssRNA/ssDNA fragments under electrostatic conditions and showed annealing and displacement of RNA strands in comparison to mutant.
  
  
  CONCLUSIONS
  Point mutation impaired RNA chaperone activity of the mutant and its interaction with nucleotides.
authors: Division of Parasitology, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow (UP) 226031, India.|Department of Biochemistry, Lucknow University, Lucknow (UP) 226007, India.
authors: Jeetendra Kumar
authors: JK
authors: Nag
authors: Division of Parasitology, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow (UP) 226031, India.|Academy of Scientific & Innovative Research, Coordination Office, Mathura Road, CRRI, Jasola, New Delhi 110020, India.
authors: Dhanvantri
authors: D
authors: Chahar
authors: Division of Parasitology, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow (UP) 226031, India.
authors: Nidhi
authors: N
authors: Shrivastava
authors: Department of Biosciences, Integral University, Lucknow (UP) 226026, India.
authors: Chhedi Lal
authors: CL
authors: Gupta
authors: Department of Biosciences, Integral University, Lucknow (UP) 226026, India.
authors: Preeti
authors: P
authors: Bajpai
authors: Department of Biochemistry, Lucknow University, Lucknow (UP) 226007, India.
authors: Deepak
authors: D
authors: Chandra
authors: Division of Parasitology, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow (UP) 226031, India.|Academy of Scientific & Innovative Research, Coordination Office, Mathura Road, CRRI, Jasola, New Delhi 110020, India.
authors: Shailja
authors: S
authors: Misra-Bhattacharya
mesh_terms: D000818:Animals; D001120:Arginine; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005075:Biological Evolution; D017178:Brugia malayi / Q000382:microbiology; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D016297:Mutagenesis, Site-Directed; D010802:Phylogeny; D017354:Point Mutation; D039861:Prokaryotic Initiation Factor-1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D016415:Sequence Alignment; D020577:Wolbachia / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D039861:Prokaryotic Initiation Factor-1; D012313:RNA; D004247:DNA; D001120:Arginine
keywords: Brugia malayi; Wolbachia; chaperone activity; fluorescence resonance energy transfer; lymphatic filariasis; mobility shift assay; translational initiation factor