title: The therapeutic effects of tuberostemonine against cigarette smoke-induced acute lung inflammation in mice.
abstract: Chronic obstructive pulmonary disease (COPD) is mainly caused by cigarette smoking and is characterized by the destruction of lung parenchyma, structural alterations of the small airways, and systemic inflammation. Tuberostemonine (TS) is an alkaloid-type phytochemical from Stemona tuberosa. In the present study, we evaluated the anti-inflammatory effect of TS in a cigarette smoke (CS)-induced mouse model of acute lung inflammation. The mice were whole-body exposed to CS or fresh air for 7 days. TS was administered by an intraperitoneal (i.p.) injection 1h before exposure to CS. To test the effects of TS, the numbers of total cells, neutrophils, macrophages and lymphocytes in the bronchoalveolar lavage (BAL) fluid were counted. Furthermore, we measured the levels of several chemokines, such as GCP-2, MIP-3α, MCP-1 and KC, in the lung tissue. The cellular profiles and histopathological analysis demonstrated that the infiltration of peribronchial and perivascular inflammatory cells significantly decreased in the TS-treated groups compared with the CS-exposure group. The TS treatment significantly ameliorated the airway epithelial thickness induced by CS exposure and caused a significant decrement in the production of chemokines in the lung. These results suggest that TS has anti-inflammatory effects against CS-induced acute lung inflammation.
authors: Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: jhkh242@naver.com.
authors: Kyung-Hwa
authors: KH
authors: Jung
authors: Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: bguswjd@naver.com.
authors: Hyunjung
authors: H
authors: Beak
authors: Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: ih_sj@naver.com.
authors: Soojin
authors: S
authors: Park
authors: Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: ssd060@naver.com.
authors: Dasom
authors: D
authors: Shin
authors: Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: Pandaluna@naver.com.
authors: Jaehoon
authors: J
authors: Jung
authors: Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: genesispsw@naver.com.
authors: Sangwon
authors: S
authors: Park
authors: Department of Korean Physiology, College of Pharmacy, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: shdwer@khu.ac.kr.
authors: Jinju
authors: J
authors: Kim
authors: Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoeki-Dong, Dongdaemoon-Gu, Seoul 130-701, Republic of Korea. Electronic address: hbae@khu.ac.kr.
authors: Hyunsu
authors: H
authors: Bae
mesh_terms: D000208:Acute Disease; D000470:Alkaloids / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects; D018925:Chemokines / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011014:Pneumonia / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D012906:Smoke / Q000009:adverse effects*; D062789:Tobacco Products / Q000032:analysis*
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D018925:Chemokines; D012906:Smoke; C034243:tuberostemonine
keywords: Chemokines; Chronic obstructive pulmonary disease (COPD); Cigarette smoke (CS); Inflammation; Tuberostemonine (PubChem CID: 100781); Tuberostemonine (TS)