title: Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR.
abstract: Nucleosome occupancy in promoter and genic regions can severely influence the transcription levels. Few methods have been established to investigate the nucleosome occupancy along the DNA. In this chapter we describe a detailed protocol to analyze the nucleosome occupancy at a specific locus using MNase-pPCR.
authors: Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany.|Chemical Genomics Centre (CGC) of the Max Planck Society, 44227, Dortmund, Germany.|MPI for Developmental Biology, 72076, Tübingen, Germany.
authors: Margaux
authors: M
authors: Kaster
authors: Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany. sascha.laubinger@zmbp.uni-tuebingen.de.|Chemical Genomics Centre (CGC) of the Max Planck Society, 44227, Dortmund, Germany. sascha.laubinger@zmbp.uni-tuebingen.de.|MPI for Developmental Biology, 72076, Tübingen, Germany. sascha.laubinger@zmbp.uni-tuebingen.de.
authors: Sascha
authors: S
authors: Laubinger
mesh_terms: D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D009707:Nucleosomes
keywords: Arabidopsis; Biotic stress; Epigenetics; MNase-qPCR; Nuclei isolation; Nucleosome occupancy