title: Quantitative profiling of sphingolipids in wild Cordyceps and its mycelia by using UHPLC-MS.
abstract: In the present study, 101 sphingolipids in wild Cordyceps and its five mycelia were quantitatively profiled by using a fully validated UHPLC-MS method. The results revealed that a general rank order for the abundance of different classes of sphingolipids in wild Cordyceps and its mycelia is sphingoid bases/ceramides > phosphosphingolipids > glycosphingolipids. However, remarkable sphingolipid differences between wild Cordyceps and its mycelia were observed. One is that sphingoid base is the dominant sphingolipid in wild Cordyceps, whereas ceramide is the major sphingolipid in mycelia. Another difference is that the abundance of sphingomyelins in wild Cordyceps is almost 10-folds higher than those in most mycelia. The third one is that mycelia contain more inositol phosphorylceramides and glycosphingolipids than wild Cordyceps. Multivariate analysis was further employed to visualize the difference among wild Cordyceps and different mycelia, leading to the identification of respective sphingolipids as potential chemical markers for the differentiation of wild Cordyceps and its related mycelia. This study represents the first report on the quantitative profiling of sphingolipids in wild Cordyceps and its related mycelia, which provided comprehensive chemical evidence for the quality control and rational utilization of wild Cordyceps and its mycelia.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
authors: Jia-Ning
authors: JN
authors: Mi
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
authors: Jing-Rong
authors: JR
authors: Wang
authors: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.|International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors: Zhi-Hong
authors: ZH
authors: Jiang
mesh_terms: D002138:Calibration; D002518:Ceramides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D032661:Cordyceps / Q000737:chemistry*; D006028:Glycosphingolipids / Q000032:analysis; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D025282:Mycelium / Q000737:chemistry*; D015203:Reproducibility of Results; D013107:Sphingolipids / Q000032:analysis*
chemical_list: D002518:Ceramides; D006028:Glycosphingolipids; D013107:Sphingolipids