title: Engineering therapeutic antibodies targeting G-protein-coupled receptors.
abstract: G-protein-coupled receptors (GPCRs) are one of the most attractive therapeutic target classes because of their critical roles in intracellular signaling and their clinical relevance to a variety of diseases, including cancer, infection and inflammation. However, high conformational variability, the small exposed area of extracellular epitopes and difficulty in the preparation of GPCR antigens have delayed both the isolation of therapeutic anti-GPCR antibodies as well as studies on the structure, function and biochemical mechanisms of GPCRs. To overcome the challenges in generating highly specific anti-GPCR antibodies with enhanced efficacy and safety, various forms of antigens have been successfully designed and employed for screening with newly emerged systems based on laboratory animal immunization and high-throughput-directed evolution.
authors: Department of Bio and Nano Chemistry, Kookmin University, Seoul, Korea.
authors: Migyeong
authors: M
authors: Jo
authors: Department of Bio and Nano Chemistry, Kookmin University, Seoul, Korea.
authors: Sang Taek
authors: ST
authors: Jung
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification; D002986:Clinical Trials as Topic; D006801:Humans; D015202:Protein Engineering / Q000379:methods; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins