title: Suppression of microglia activation after hypoxia-ischemia results in age-dependent improvements in neurologic injury.
abstract: We previously found increased microglial proliferation and pro-inflammatory cytokine release in infant mice compared to juvenile mice after hypoxia-ischemia (HI). The aim of the current study was to assess for differences in the effect of microglial suppression on HI-induced brain injury in infant and juvenile mice. HI was induced in neonatal (P9) and juvenile (P30) mice and minocycline or vehicle was administered at 2h and 24h post-HI. P9 minocycline-treated mice demonstrated early but transient improvements in neurologic injury, while P30 minocycline-treated mice demonstrated sustained improvements in cerebral atrophy and Morris Water Maze performance at 60days post-HI.
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: cikla@wisc.edu.
authors: Ulas
authors: U
authors: Cikla
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: chanana@pediatrics.wisc.edu.
authors: Vishal
authors: V
authors: Chanana
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: dkintner@pediatrics.wisc.edu.
authors: Douglas B
authors: DB
authors: Kintner
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: lcovert@wisc.edu.
authors: Lucia
authors: L
authors: Covert
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: tdewall@wisc.edu.
authors: Taylor
authors: T
authors: Dewall
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: awaldman@wisc.edu.
authors: Alex
authors: A
authors: Waldman
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA. Electronic address: prawley@wisc.edu.
authors: Paul
authors: P
authors: Rowley
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA; Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA. Electronic address: cengiz@pediatrics.wisc.edu.
authors: Pelin
authors: P
authors: Cengiz
authors: Waisman Center, University of Wisconsin, Madison, WI 53705, USA; Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA. Electronic address: ferrazzano@pediatrics.wisc.edu.
authors: Peter
authors: P
authors: Ferrazzano
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000473:pathology*; D001930:Brain Injuries / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology*; D039481:CD11b Antigen / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D007839:Functional Laterality; D020925:Hypoxia-Ischemia, Brain / Q000150:complications* / Q000473:pathology; D007859:Learning Disabilities / Q000188:drug therapy / Q000209:etiology; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D018782:Maze Learning; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008911:Minocycline / Q000627:therapeutic use; D009460:Neurologic Examination; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D039481:CD11b Antigen; D017493:Leukocyte Common Antigens; D008911:Minocycline
keywords: Cerebral atrophy; Microglia; Neonatal hypoxia–ischemia; Neuroinflammation