title: Facile rhenium-peptide conjugate synthesis using a one-pot derived Re(CO)3 reagent.
abstract: We have synthesized two Re(CO)3-modified lysine complexes (1 and 2), where the metal is attached to the amino acid at the Nε position, via a one-pot Schiff base formation reaction. These compounds can be used in the solid phase synthesis of peptides, and to date we have produced four conjugate systems incorporating neurotensin, bombesin, leutenizing hormone releasing hormone, and a nuclear localization sequence. We observed uptake into human umbilical vascular endothelial cells as well as differential uptake depending on peptide sequence identity, as characterized by fluorescence and rhenium elemental analysis.
authors: Department of Chemistry, University of Akron, OH 44325-3601, USA. ziegler@uakron.edu.
authors: Kullapa
authors: K
authors: Chanawanno
authors: Vinay
authors: V
authors: Kondeti
authors: Joel
authors: J
authors: Caporoso
authors: Sailaja
authors: S
authors: Paruchuri
authors: Thomas C
authors: TC
authors: Leeper
authors: Richard S
authors: RS
authors: Herrick
authors: Christopher J
authors: CJ
authors: Ziegler
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000378:metabolism / Q000633:toxicity; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012211:Rhenium / Q000737:chemistry* / Q000378:metabolism; D012545:Schiff Bases / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques
chemical_list: D056831:Coordination Complexes; D010455:Peptides; D012545:Schiff Bases; D012211:Rhenium