title: Histone deacetylase 5 promotes Wilms' tumor cell proliferation through the upregulation of c-Met.
abstract: The histone deacetylase (HDAC) family is comprised of enzymes, which are involved in modulating the majority of critical cellular processes, including transcriptional regulation, apoptosis, proliferation and cell cycle progression. However, the biological function of HDAC5 in Wilms' tumor remains to be fully elucidated. The present study aimed to investigate the expression and function of HDAC5 in Wilm's tumor. It was demonstrated that the mRNA and protein levels of HDAC5 were upregulated in human Wilms' tumor tissues. Overexpression of HDAC5 in G401 cells was observed to significantly promote cellular proliferation, as demonstrated by the results of an MTT assay and bromodeoxyuridine incorporation assay. By contrast, HDAC5 knockdown using small interfering RNA suppressed the proliferation of the G401 cells. At the molecular level, the present study demonstrated that HDAC5 promoted the expression of c‑Met, which has been previously identified as an oncogene. In addition, downregulation of c‑Met inhibited the proliferative effects of HDAC5 in human Wilms' tumor cells. Taken together, these results suggested that HDAC5 promotes cellular proliferation through the upregulation of c‑Met, and may provide a novel therapeutic target for the treatment of patients with Wilms' tumor.
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China.
authors: Xu
authors: X
authors: Cao
authors: Department of Pediatric Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China.
authors: De-Hong
authors: DH
authors: Liu
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China.
authors: Yun
authors: Y
authors: Zhou
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China.
authors: Xiang-Ming
authors: XM
authors: Yan
authors: Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu 215004, P.R. China.
authors: Li-Qun
authors: LQ
authors: Yuan
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China.
authors: Jian
authors: J
authors: Pan
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China.
authors: Ming-Cui
authors: MC
authors: Fu
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China.
authors: Ting
authors: T
authors: Zhang
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou, Jiangsu 215003, P.R. China.
authors: Jian
authors: J
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000473:pathology; D008297:Male; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D009396:Wilms Tumor / Q000201:enzymology* / Q000473:pathology
chemical_list: C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases; C404500:HDAC5 protein, human; D006655:Histone Deacetylases