title: Polyphenolic-polysaccharide conjugates from plants of Rosaceae/Asteraceae family as potential radioprotectors.
abstract: Polyphenolic-polysaccharide macromolecular, water-soluble glycoconjugates, isolated from the selected medicinal plants of Rosaceae/Asteraceae family: from leaves of Fragaria vesca L., Rubus plicatus Whe. et N. E., and from flowering parts of Sanguisorba officinalis L., and Erigeron canadensis L., were investigated for their ability to protect proteins and lipids of human plasma against γ-radiation-induced oxidative damage. Treatment of plasma with plant conjugates (6, 30, 150 μg/ml) prior exposure to 100 Gy radiation resulted in a significant inhibition of lipid peroxidation, evaluated by TBARS levels; conjugates isolated from E. canadensis and R. plicatus and a reference flavonoid quercetin showed similar high potential (approx. 70% inhibition, at 6 μg/ml). The conjugates prevented radiation-induced oxidation of protein thiols and significantly improved plasma total antioxidant capacity, estimated with Ellman's reagent and ABTS(.+) assay, respectively. The results demonstrate by the first time a significant radioprotective capability of the polyphenolic-polysaccharide conjugates isolated from E. canadensis, R. plicatus, S. officinalis and to the less extent from F. vesca. The abilities of these substances to inhibit radiation-induced lipid peroxidation and thiol oxidation in plasma seems to be mediated, but not limited to ROS scavenging activity.
authors: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: zbikow@biol.uni.lodz.pl.
authors: Halina Malgorzata
authors: HM
authors: Zbikowska
authors: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors: Magdalena
authors: M
authors: Szejk
authors: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors: Joanna
authors: J
authors: Saluk
authors: Division of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors: Izabela
authors: I
authors: Pawlaczyk-Graja
authors: Division of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors: Roman
authors: R
authors: Gancarz
authors: Chemistry Department, Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.
authors: Alicja Klaudia
authors: AK
authors: Olejnik
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry* / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry*; D011837:Radiation-Protective Agents / Q000737:chemistry* / Q000494:pharmacology*; D027824:Rosaceae / Q000737:chemistry*
chemical_list: D059808:Polyphenols; D011134:Polysaccharides; D011837:Radiation-Protective Agents
keywords: Polyphenolic–polysaccharide antioxidant; Radioprotector; γ-Radiation