title: Comparison of the antigenic relationship between Japanese encephalitis virus genotypes 1 and 3.
abstract: OBJECTIVE
  The Japanese encephalitis virus (JEV) genotype circulating in Korea has changed from G3 to G1. Therefore, the purpose of this study was to compare the antigenic relationship between the two genotypes by using antibody tests.
  
  
  METHODS
  Blood samples from 42 sows and 216 horses were collected, and their seroprevalence was monitored using the hemagglutination inhibition and virus neutralization tests. Antisera against JEV G1 and G3 were isolated and prepared from guinea pigs. The cross-reactivity of these two viruses was then compared using the neutralizing antibody test.
  
  
  RESULTS
  We found that there was a difference in the seropositive ratios of JEV G1 and G3. However, the difference was dependent on the antibody test used. There was also an observed difference in the antigenicity between the two genotypes, as ascertained using the neutralizing antibody test.
  
  
  CONCLUSIONS
  There is an evident difference in JEV antigenicity between the genotypes G1 and G3. Therefore, we propose monitoring of the seroprevalence of JEV, and reevaluating the antigenicity of the current vaccine by using the relevant tests.
authors: Research Unit, Green Cross Veterinary Products, Yongin, Korea.
authors: Bo-Kyu
authors: 0000-0001-5840-468X
authors: BK
authors: Kang
authors: Research Unit, Green Cross Veterinary Products, Yongin, Korea.
authors: Jeong-Min
authors: 0000-0003-4272-1220
authors: JM
authors: Hwang
authors: Research Unit, Green Cross Veterinary Products, Yongin, Korea.
authors: Hyoungjoon
authors: 0000-0001-6211-8758
authors: H
authors: Moon
authors: Research Unit, Green Cross Veterinary Products, Yongin, Korea.
authors: Sang-Yoon
authors: 0000-0001-6608-724X
authors: SY
authors: Han
authors: Research Unit, Green Cross Veterinary Products, Yongin, Korea.
authors: Jong-Man
authors: 0000-0001-8479-0393
authors: JM
authors: Kim
authors: Animal and Plant Quarantine Agency, Anyang, Korea.
authors: Dong-Kun
authors: 0000-0001-5765-3043
authors: DK
authors: Yang
authors: Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors: Bong-Kyun
authors: 0000-0002-4301-8740
authors: BK
authors: Park
authors: College of Pharmacy, Korea University, Sejong, Korea.
authors: Daesub
authors: 0000-0002-2759-1061
authors: D
authors: Song
keywords: Cross reaction; Genotype; Japanese encephalitis virus; Seroprevalence