title: Quantitative Proteomic Analysis Reveals Populus cathayana Females Are More Sensitive and Respond More Sophisticatedly to Iron Deficiency than Males.
abstract: Previous studies have shown that there are significant sexual differences in the morphological and physiological responses of Populus cathayana Rehder to nitrogen and phosphorus deficiencies, but little is known about the sex-specific differences in responses to iron deficiency. In this study, the effects of iron deficiency on the morphology, physiology, and proteome of P. cathayana males and females were investigated. The results showed that iron deficiency (25 days) significantly decreased height growth, photosynthetic rate, chlorophyll content, and tissue iron concentration in both sexes. A comparison between the sexes indicated that iron-deficient males had less height inhibition and photosynthesis system II or chloroplast ultrastructural damage than iron-deficient females. iTRAQ-based quantitative proteomic analysis revealed that 144 and 68 proteins were decreased in abundance (e.g., proteins involved in photosynthesis, carbohydrate and energy metabolism, and gene expression regulation) and 78 and 39 proteins were increased in abundance (e.g., proteins involved in amino acid metabolism and stress response) according to the criterion of ratio ≥1.5 in females and males, respectively. A comparison between the sexes indicated that iron-deficient females exhibited a greater change in the proteins involved in photosynthesis, carbon and energy metabolism, the redox system, and stress responsive proteins. This study reveals females are more sensitive and have a more sophisticated response to iron deficiency compared with males and provides new insights into differential sexual responses to nutrient deficiency.
authors: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences , Chengdu 610041, China.
authors: Sheng
authors: S
authors: Zhang
authors: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences , Chengdu 610041, China.|University of Chinese Academy of Sciences , Beijing 100039, China.
authors: Yunxiang
authors: Y
authors: Zhang
authors: Henan Polytechnic , Zhengzhou 450046, China.
authors: Yanchun
authors: Y
authors: Cao
authors: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences , Chengdu 610041, China.
authors: Yanbao
authors: Y
authors: Lei
authors: Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences , Chengdu 610041, China.
authors: Hao
authors: H
authors: Jiang
mesh_terms: D004734:Energy Metabolism; D000090463:Iron Deficiencies; D010084:Oxidation-Reduction; D010788:Photosynthesis; D010940:Plant Proteins / Q000032:analysis; D032107:Populus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D040901:Proteomics; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins
keywords: dioecious; iron deficiency; plant proteomics; sexual difference; woody plant