title: In-situ Hybridization for the Detection of Sacbrood Virus in Infected Larvae of the Honey Bee (Apis cerana).
abstract: The aim of this study was to develop and use in-situ hybridization (ISH) for the detection and localization of the sacbrood virus (SBV) in Korean honey bee (Apis cerana) larvae that were infected naturally with SBV. A 258 base pair cDNA probe for SBV was generated by polymerase chain reaction. Cells positive for viral genome typically showed a dark brown reaction in the cytoplasm. SBV was detected consistently in trophocytes and urocytes. The ISH was successfully applied to routinely fixed and processed tissues and thus should prove helpful in the diagnosis and characterization of viral distribution in infected larvae.
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
authors: C
authors: C
authors: Park
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
authors: H S
authors: HS
authors: Kang
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
authors: J
authors: J
authors: Jeong
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
authors: I
authors: I
authors: Kang
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
authors: K
authors: K
authors: Choi
authors: National Veterinary Research Quarantine Service, 480 Anyang 6-dong, Anyang-City, Republic of Korea.
authors: M-S
authors: MS
authors: Yoo
authors: National Veterinary Research Quarantine Service, 480 Anyang 6-dong, Anyang-City, Republic of Korea.
authors: Y-H
authors: YH
authors: Kim
authors: National Veterinary Research Quarantine Service, 480 Anyang 6-dong, Anyang-City, Republic of Korea.
authors: S-W
authors: SW
authors: Kang
authors: Department of Life Science, College of Natural Science, Kyonggi University, Suwon, Republic of Korea.
authors: H-Y
authors: HY
authors: Lim
authors: Department of Life Science, College of Natural Science, Kyonggi University, Suwon, Republic of Korea.
authors: B-S
authors: BS
authors: Yoon
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea. Electronic address: swine@snu.ac.kr.
authors: C
authors: C
authors: Chae
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D017403:In Situ Hybridization; D007814:Larva / Q000821:virology*; D010849:Picornaviridae; D010850:Picornaviridae Infections / Q000662:veterinary*; D016133:Polymerase Chain Reaction
keywords: Apis cerana; Iflavirus; in-situ hybridization; sacbrood virus