title: Daidzein-loaded nanostructured lipid carriers-PLGA nanofibers for transdermal delivery.
abstract: Daidzein is one of the most effective candidates for treating cardiovascular and cerebrovascular disease. However, considering its poor oral absorption and limited bioavailability, daidzein-loaded nanostructured lipid carriers-PLGA nanofibers were designed to handle the drawbacks. Daidzein-NLCs were successfully prepared by an emulsification and low-temperature solidification method. The physicochemical characteristics of NLCs were evaluated afterwards. Based on the preparation of daidzein-loaded NLCs, Daidzein-NLCs-nanofibers were optimized by electrospinning and were observed under Scanning Electron Microscope to capture the appearance. The sustained release profile of daidzein from Daidzein-NLCs-nanofibers in vivo was best fitted to the Kormeyer-Peppas equation. The in vitro skin permeable behavior showed the cumulative amount of daidzein from Daidzein-NLCs-nanofibers reached 21.71 μg cm(-2) at 60 h, which was 3.78 times higher than pure daidzein solution. It demonstrated that the Daidzein-NLCs-nanofibers could significantly enhance the transported amount of drug. Confocal Laser Scanning Microscopy resulting images revealed a more effective content accumulation of Daidzein-NLCs-nanofibers than Daidzein-NLCs in epidermis. In vivo study indicated that Daidzein-NLCs-nanofibers had better skin retention than Daidzein-NLCs in the long term. The skin irritation experiment showed a positive result with no obvious stimulus observed. These results suggested that Daidzein-NLCs-nanofibers could be a potential candidate for transdermal delivery.
authors: School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan, Road, Shanghai 200240, China.
authors: Jia
authors: J
authors: Song
authors: School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan, Road, Shanghai 200240, China.
authors: Xiucong
authors: X
authors: Fan
authors: School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan, Road, Shanghai 200240, China. Electronic address: qshen@sjtu.edu.cn.
authors: Qi
authors: Q
authors: Shen
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001381:Azepines / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D065546:Drug Liberation; D066298:In Vitro Techniques; D007529:Isoflavones / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D008055:Lipids / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D008297:Male; D057139:Nanofibers / Q000008:administration & dosage* / Q000737:chemistry; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption; D023422:Skin Irritancy Tests
chemical_list: D001381:Azepines; D004337:Drug Carriers; D007529:Isoflavones; D008055:Lipids; C035229:laurocapram; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C004742:daidzein
keywords: Azone (PubChem CID: 42981); Daidzein; Daidzein (PubChem CID: 5281708); Glycerin monostearate (PubChem CID: 24699); Lecithin (PubChem CID: 5287971); Nanofibers; Nanostructured lipid carriers; PLGA (PubChem CID: 23111554); PLGA- nanofibers; Transdermal drug delivery