title: Mechanistic investigation of the on-surface enzymatic digestion (oSED) protein adsorption detection method using targeted mass spectrometry.
abstract: This study describes our efforts to study some of the mechanistic aspects of the earlier established on-surface enzymatic digestion (oSED) method. In a multitude of application areas, it has become important to be able to fully characterize and understand selective protein adsorption to biomaterial surfaces for various applications, including biomedicine (implants), nanotechnology (microchip surfaces and sensors) and materials sciences. Herein, the investigation of the mechanistic aspects was based on microdialysis catheter tubes that were flushed with controlled protein solutions mimicking the extracellular fluid of the brain. The protein adsorption properties were monitored using high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS) with a targeted method. The temporally resolved results show that most proteins stay adsorbed onto the surface during the entire digestion process and are only cut away piece by piece, whereas smaller proteins and peptides seem to desorb rather easily from the surface. This information will simplify the interpretation of data generated using the oSED method and can also be used for the characterization of the physicochemical properties controlling the adsorption of individual proteins to specific surfaces.
authors: Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden. sara.lind@kemi.uu.se.
authors: Torgny
authors: T
authors: Undin
authors: Department of Engineering Sciences, Uppsala University, PO Box 534, SE-751 21 Uppsala, Sweden.
authors: Andreas
authors: A
authors: Dahlin
authors: Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden. sara.lind@kemi.uu.se.
authors: Katarina
authors: K
authors: Hörnaeus
authors: Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden. sara.lind@kemi.uu.se.
authors: Jonas
authors: J
authors: Bergquist
authors: Department of Chemistry-BMC, Analytical Chemistry and Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden. sara.lind@kemi.uu.se.
authors: Sara Bergström
authors: SB
authors: Lind
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D002853:Chromatography, Liquid; D006801:Humans; D017551:Microdialysis; D036103:Nanotechnology; D059748:Proteolysis; D013499:Surface Properties; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism*
chemical_list: D002556:Cerebrospinal Fluid Proteins; D014357:Trypsin