title: Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters: Results of human intervention trials.
abstract: METHODS
  Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans.
  
  
  RESULTS
  The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention.
  
  
  CONCLUSIONS
  Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage.
authors: Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors: Franziska
authors: F
authors: Ferk
authors: Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors: Miroslav
authors: M
authors: Mišík
authors: Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors: Armen
authors: A
authors: Nersesyan
authors: Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors: Christoph
authors: C
authors: Pichler
authors: Department for Clinical Pharmacy and Diagnostics, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
authors: Walter
authors: W
authors: Jäger
authors: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria.
authors: Thomas
authors: T
authors: Szekeres
authors: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austria.
authors: Rodrig
authors: R
authors: Marculescu
authors: Laboratory of Clinical Pharmacology Copenhagen, Copenhagen, Denmark.
authors: Henrik E
authors: HE
authors: Poulsen
authors: Laboratory of Clinical Pharmacology Copenhagen, Copenhagen, Denmark.
authors: Trine
authors: T
authors: Henriksen
authors: Department of Public Health and Pediatrics, University of Torino, Torino, Italy.
authors: Roberto
authors: R
authors: Bono
authors: Department of Public Health and Pediatrics, University of Torino, Torino, Italy.
authors: Valeria
authors: V
authors: Romanazzi
authors: Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors: Halh
authors: H
authors: Al-Serori
authors: Hopsteiner, Hallertauer Hopfenveredelung GmbH, Mainburg, Germany.
authors: Martin
authors: M
authors: Biendl
authors: Department of Nutritional Sciences, University of Vienna, Vienna, Austria.
authors: Karl-Heinz
authors: KH
authors: Wagner
authors: Center for Public Health, Institute of Environmental Health, Medical University of Vienna, Vienna, Austria.
authors: Michael
authors: M
authors: Kundi
authors: Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, Vienna, Austria.
authors: Siegfried
authors: S
authors: Knasmüller
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D005419:Flavonoids / Q000097:blood / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011427:Propiophenones / Q000097:blood / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D005419:Flavonoids; D011427:Propiophenones; D020011:Protective Agents; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; C104536:xanthohumol
keywords: 8-oxoGuo; 8-oxodG; DNA damage; SCGE assay; xanthohumol