title: microRNA-497 inhibits invasion and metastasis of colorectal cancer cells by targeting vascular endothelial growth factor-A.
abstract: OBJECTIVE
  microRNAs (miRNAs), are non-coding RNAs that regulate gene expression, and are involved in tumour development. The aim of this study was to investigate microRNA-497 (miR-497) expression and its role in development of colorectal cancer (CRC).
  
  
  METHODS
  RT-PCR was performed to detect expression of miR-497 in CRC cell lines (HCT8, LOVO, Ls-174, HCT116 and HT29) and in clinical cancer specimens. To further understand its role, we restored expression of miR-497 in the HCT116 cell line by transfection with miR-497 mimics or inhibitors. Effects of miR-497 on cell proliferation, migration and invasion of targets were also determined both in vitro and in vivo.
  
  
  RESULTS
  miR-497 expression decreased in 34 CRC tissues compared to non-tumour tissues and in tumour cell lines. Overexpression of miR-497 did not inhibit cancer cell growth but suppressed metastasis and invasion both in vitro and in vivo. Vascular endothelial growth factor-A (VEGF-A) was confirmed to be a target of miR-497. Furthermore, we found overexpression of miR-497 altered expression of key molecules of the VEGF-A/ERK/MMP-9 signalling pathway.
  
  
  CONCLUSIONS
  Thus our results provide evidence that miR-497 might function as a metastasis suppressor in CRC. Targeting miR-497 may provide a strategy for blocking its metastasis.
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Yanyan
authors: Y
authors: Qiu
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Hui
authors: H
authors: Yu
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Xiaojing
authors: X
authors: Shi
authors: Cancer Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Ke
authors: K
authors: Xu
authors: Cancer Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Qingfeng
authors: Q
authors: Tang
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Bo
authors: B
authors: Liang
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Songjiao
authors: S
authors: Hu
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Yijie
authors: Y
authors: Bao
authors: Cancer Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Jianhua
authors: J
authors: Xu
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Jie
authors: J
authors: Cai
authors: Department of Kidney, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Wen
authors: W
authors: Peng
authors: Department of Gastroenterology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Qin
authors: Q
authors: Cao
authors: Department of General Surgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.|Cancer Institute of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Peihao
authors: P
authors: Yin
mesh_terms: D000818:Animals; D001483:Base Sequence; D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: C559640:MIRN497 microRNA, human; D035683:MicroRNAs; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A