title: Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes.
abstract: Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. Here we provide details of our massive set of supporting biochemical fractionation data available via ProteomeXchange (PXD002319-PXD002328), PPIs via BioGRID (185267); and interaction network projections via (http://metazoa.med.utoronto.ca) made fully accessible to allow further exploration. The datasets here are related to the research article on metazoan macromolecular complexes in Nature [1].
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
authors: Sadhna
authors: S
authors: Phanse
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors: Cuihong
authors: C
authors: Wan
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
authors: Blake
authors: B
authors: Borgeson
authors: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors: Fan
authors: F
authors: Tu
authors: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors: Kevin
authors: K
authors: Drew
authors: Department of Medical Biophysics, Toronto, Ontario, Canada.
authors: Greg
authors: G
authors: Clark
authors: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
authors: Xuejian
authors: X
authors: Xiong
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
authors: Olga
authors: O
authors: Kagan
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors: Julian
authors: J
authors: Kwan
authors: Department of Medical Biophysics, Toronto, Ontario, Canada.
authors: Alexandr
authors: A
authors: Bezginov
authors: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
authors: Kyle
authors: K
authors: Chessman
authors: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
authors: Swati
authors: S
authors: Pal
authors: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
authors: Graham
authors: G
authors: Cromar
authors: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors: Ophelia
authors: O
authors: Papoulas
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
authors: Zuyao
authors: Z
authors: Ni
authors: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors: Daniel R
authors: DR
authors: Boutz
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
authors: Snejana
authors: S
authors: Stoilova
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
authors: Pierre C
authors: PC
authors: Havugimana
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
authors: Xinghua
authors: X
authors: Guo
authors: Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada.
authors: Ramy H
authors: RH
authors: Malty
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors: Mihail
authors: M
authors: Sarov
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors: Jack
authors: J
authors: Greenblatt
authors: Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada.
authors: Mohan
authors: M
authors: Babu
authors: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
authors: W Brent
authors: WB
authors: Derry
authors: Department of Medical Biophysics, Toronto, Ontario, Canada.
authors: Elisabeth R
authors: ER
authors: Tillier
authors: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
authors: John B
authors: JB
authors: Wallingford
authors: Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
authors: John
authors: J
authors: Parkinson
authors: Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
authors: Edward M
authors: EM
authors: Marcotte
authors: Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors: Andrew
authors: A
authors: Emili
keywords: Biochemical; Fractionation; Metazoa; Protein complexes; Proteomics