title: Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters.
abstract: Laser capture microdissection (LCM) is a powerful technique for harvesting specific cells from a heterogeneous population. As each cell and tissue has its unique genetic, proteomic, and metabolic profile, the use of homogeneous samples is important for a better understanding of complex processes in both animal and plant systems. In case of plants, LCM is very suitable as the highly regular tissue organization and stable cell walls from these organisms enable visual identification of various cell types without staining of tissue sections, which can prevent some downstream analysis. Considering the applicability of LCM to any plant species, here we provide a step-by-step protocol for selecting specific cells or tissues through this technology.
authors: Center for Biotechnology, Federal University of Rio Grande do Sul, 15005, 91501-970, Porto Alegre, RS, Brazil.
authors: Márcia R
authors: MR
authors: de Almeida
authors: Department of Plant Science, McGill University, 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, QC, Canada, H9X 3V9. martina.stromvik@mcgill.ca.
authors: Martina V
authors: MV
authors: Strömvik
mesh_terms: D061346:Laser Capture Microdissection / Q000379:methods* / Q000592:standards
keywords: Cell-specific technique; Cryosections; Microgenomics; Microscopy; Plant cells