title: Determination of free amino acids in burley tobacco by high performance liquid chromatography.
abstract: A reversed-phase high performance liquid chromatographic method was developed for determining free amino acids in burley tobacco. The test was done by OPA/3-mercaptopropionic acid as the pre-column derivatizing reagent. Chromatographic column was Elitte C(18) column (4.6 mm × 250 mm i.d., 5 μm). Mobile phase A was 18 mol/l NaAc (pH7.2) including 0.002%(v/v) triethylamine and 0.3%(v/v) furanidine. Mobile phase B was 100 mol/l NaAc (pH7.2)-acetonitrile-methanol (v/v = 1:2:2). The column temperature was 40 °C and the flow rate was 1.0 ml/min. The fluorescence detector was used with 350 nm excitation wave length and 450 nm emission wave length. The average recoveries of the method ranged from 95.3-100.7% with the relative standard deviation of 2.32-9.24%. The method is simple, accurate and has good repeatability. The results of the determination of seventeen kinds of free amino acids in burley leaves were produced by the way of different ratios of cake fertilizer and inorganic fertilizer. The results show that Aspartic acid has the highest content however ratio of cake fertilizer and inorganic fertilizer. The contents of most of the free amino acids are increased and then gradually decreased with the increase in organic manure. The contents of most of the free amino acids are very close at 15:85% ratio and 30:70% ratio of cake fertilizer and inorganic fertilizer. The total amount of free amino acids is the highest at 30:70% ratio of cake fertilizer and inorganic fertilizer. Considering comprehensively, the quality of burley leaves is the best at 30:70% ratio of cake fertilizer and inorganic fertilizer.
authors: College of Tobacco Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou 450002, Henan, PR China.
authors: Yanqiu
authors: Y
authors: Jing
authors: College of Tobacco Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou 450002, Henan, PR China.
authors: Baolin
authors: B
authors: Zhang
authors: College of Tobacco Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou 450002, Henan, PR China.
authors: Xiuxiu
authors: X
authors: Yuan
authors: Technology Center for Tobacco Industrial Company in Gansu, No. 111 Binhe South Road, Lanzhou 730050, Gansu, PR China.
authors: Yuzhen
authors: Y
authors: Gao
authors: Henan Tobacco Industry Company, Zhengzhou 450000, Henan, PR China.
authors: Ping
authors: P
authors: Lu
authors: China National Tobacco Corporation of Guangxi Province, Nanning 530023, Guangxi, PR China.
authors: Weifeng
authors: W
authors: Wang
authors: China National Tobacco Corporation of Henan Province, Zhengzhou 450002, Henan, PR China.
authors: Min
authors: M
authors: Xu
keywords: Burley leaves; Cake fertilizer; Different ratio; Free amino acids; Inorganic fertilizer