title: Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence.
abstract: Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment.
authors: Department of Psychiatry, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.|Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, New York, United States of America.
authors: Zachary J
authors: ZJ
authors: Farino
authors: Department of Psychiatry, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.|Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, New York, United States of America.
authors: Travis J
authors: TJ
authors: Morgenstern
authors: Research Department, Cisbio Bioassays, Codolet, France.
authors: Julie
authors: J
authors: Vallaghe
authors: Research Department, Cisbio Bioassays, Codolet, France.
authors: Nathalie
authors: N
authors: Gregor
authors: Department of Psychiatry, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.|Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, New York, United States of America.|Department of Pharmacology, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.
authors: Prashant
authors: P
authors: Donthamsetti
authors: Division of Endocrinology, Department of Medicine, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.
authors: Paul E
authors: PE
authors: Harris
authors: Research Department, Cisbio Bioassays, Codolet, France.
authors: Nicolas
authors: N
authors: Pierre
authors: Department of Psychology, Stern College for Women, Yeshiva University, New York, New York, United States of America.
authors: Robin
authors: R
authors: Freyberg
authors: Research Department, Cisbio Bioassays, Codolet, France.
authors: Fabienne
authors: F
authors: Charrier-Savournin
authors: Department of Psychiatry, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.|Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, New York, United States of America.|Department of Pharmacology, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.
authors: Jonathan A
authors: JA
authors: Javitch
authors: Department of Psychiatry, College of Physicians & Surgeons, Columbia University, New York, New York, United States of America.|Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, New York, United States of America.
authors: Zachary
authors: Z
authors: Freyberg
mesh_terms: D000818:Animals; D001971:Bromocriptine / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D005453:Fluorescence; D007328:Insulin / Q000032:analysis* / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000819:agonists / Q000378:metabolism; D050637:Receptors, Dopamine D3 / Q000819:agonists / Q000378:metabolism
chemical_list: D007328:Insulin; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D001971:Bromocriptine