title: Detection of an Immunogenic HERV-E Envelope with Selective Expression in Clear Cell Kidney Cancer.
abstract: VHL-deficient clear cell renal cell carcinomas (ccRCC), the most common form of kidney cancer, express transcripts derived from the novel human endogenous retrovirus HERV-E (named CT-RCC HERV-E). In this study, we define a transcript encoding the entire envelope gene of HERV-E as expressed selectively in ccRCC tumors, as distinct from normal kidney tissues or other tumor types. Sequence analysis of this envelope transcript revealed long open reading frames encoding putative surface and transmembrane envelope proteins. Retroviral envelopes are known to be capable of eliciting immunity in humans. Accordingly, we found that HLA-A*0201-restricted peptides predicted to be products of the CT-RCC HERV-E envelope transcript-stimulated CD8(+) T cells, which could recognize HLA-A*0201-positive HERV-E-expressing kidney tumor cells. Overall, our results offer evidence of unique HERV-E envelope peptides presented on the surface of ccRCC cells, offering potentially useful tumor-restricted targets for T-cell-based immunotherapy of kidney cancer. Cancer Res; 76(8); 2177-85. ©2016 AACR.
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Elena
authors: E
authors: Cherkasova
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Claire
authors: C
authors: Scrivani
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Susan
authors: S
authors: Doh
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Quinn
authors: Q
authors: Weisman
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Yoshiyuki
authors: Y
authors: Takahashi
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Nanae
authors: N
authors: Harashima
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Hisayuki
authors: H
authors: Yokoyama
authors: Urologic Oncology Branch, NCI, NIH, Bethesda, Maryland.
authors: Ramaprasad
authors: R
authors: Srinivasan
authors: Urologic Oncology Branch, NCI, NIH, Bethesda, Maryland.
authors: W Marston
authors: WM
authors: Linehan
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland.
authors: Michael I
authors: MI
authors: Lerman
authors: Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland. childsr@mail.nih.gov.
authors: Richard W
authors: RW
authors: Childs
mesh_terms: D000595:Amino Acid Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000821:virology*; D045744:Cell Line, Tumor; D020077:Endogenous Retroviruses / Q000235:genetics / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005814:Genes, Viral; D006801:Humans; D007680:Kidney Neoplasms / Q000276:immunology / Q000821:virology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D014759:Viral Envelope Proteins