title: Rhenium-coated glass beads for intracolonic administration attenuate TNBS-induced colitis in mice: Proof-of-Concept Study.
abstract: In search for novel effective treatments in inflammatory bowel diseases, a new strategy employing glass beads coated with rhenium nanolayer has been developed and validated in the mouse model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Briefly, mice were randomly divided into 5 experimental groups: control (vehicle alone, Group 1); control treated with rhenium-coated glass beads (Group 2); TNBS (Group 3); TNBS treated with rhenium-coated glass beads (Group 4); and TNBS treated with uncoated glass beads (Group 5). Mice from Group 2, 4 and 5 were treated with respective beads (once daily, 5 beads / animal, i.c.) between D3-D6 post-TNBS/vehicle and evaluation of colonic damage was performed on D7, based on macroscopic scoring and clinical parameters. Severe colonic inflammation developed in post-TNBS mice (Group 3) [P <0.001 vs. control (Group 1) for macroscopic score], which was significantly attenuated by treatment with rhenium-coated glass beads (Group 4) [P <0.01 vs. TNBS (Group 3), for macroscopic score]. Neither rhenium-coated glass beads had any effect in control animals (Group 2), nor uncoated glass beads influenced TNBS-induced colitis (Group 5). In conclusion, a novel and attractive strategy for the treatment of colonic inflammation has been proposed; therapy with rhenium-coated glass beads already proved effective in the mouse model of TNBS-induced colitis, now requires further characterization in clinical conditions.
authors: Department of Vehicles and Fundamentals of Machine Design, Lodz University of Technology, Lodz, Poland.
authors: Krzysztof
authors: K
authors: Siczek
authors: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.
authors: Hubert
authors: H
authors: Zatorski
authors: Wojciech
authors: W
authors: Pawlak
authors: Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland. jakub.fichna@umed.lodz.pl.
authors: Jakub
authors: J
authors: Fichna
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000494:pharmacology*; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000517:prevention & control; D003106:Colon / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D005898:Glass; D051379:Mice; D008807:Mice, Inbred BALB C; D012211:Rhenium; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage
chemical_list: D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D005765:Gastrointestinal Agents; D012211:Rhenium; D014302:Trinitrobenzenesulfonic Acid
keywords: Crohn’s disease; glass bead; mouse model of colitis; rare elements; rhenium; transition metal