title: Versatile Types of MRI-Visible Cationic Nanoparticles Involving Pullulan Polysaccharides for Multifunctional Gene Carriers.
abstract: Owing to the low cytotoxicity and excellent biocompatibility, polysaccharides are good candidates for the development of promising biomaterials. In this paper, a series of magnetic resonance imaging (MRI)-visible cationic polymeric nanoparticles involving liver cell-targeting polysaccharides were flexibly designed for multifunctional gene delivery systems. The pullulan-based vector (PuPGEA) consisting of one liver cell-targeting pullulan backbone and ethanolamine-functionalized poly(glycidyl methacrylate) (denoted by BUCT-PGEA) side chains with abundant hydroxyl units and secondary amine was first prepared by atom transfer radical polymerization. The resultant cationic nanoparticles (PuPGEA-GdL or PuPGEA-GdW) with MRI functions were produced accordingly by assembling PuPGEA with aminophenylboronic acid-modified Gd-DTPA (GdL) or GdW10O36(9-) (GdW) via the corresponding etherification or electrostatic interaction. The properties of the PuPGEA-GdL and PuPGEA-GdW nanoparticles including pDNA condensation ability, cytotoxicity, gene transfection, cellular uptake, and in vitro and in vivo MRI were characterized in details. Such kinds of cationic nanoparticles exhibited good performances in gene transfection in liver cells. PuPGEA-GdW demonstrated much better MRI abilities. The present design of PuPGEA-based cationic nanoparticles with the liver cell-targeting polysaccharides and MRI contrast agents would shed light on the exploration of tumor-targetable multifunctional gene delivery systems.
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029 China.|Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education , Beijing 100029 China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors: Yajun
authors: Y
authors: Huang
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029 China.|Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education , Beijing 100029 China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors: Hao
authors: H
authors: Hu
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029 China.|Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education , Beijing 100029 China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors: Rui-Quan
authors: RQ
authors: Li
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029 China.|Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education , Beijing 100029 China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors: Bingran
authors: B
authors: Yu
authors: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029 China.|Key Laboratory of Carbon Fiber and Functional Polymers (Beijing University of Chemical Technology), Ministry of Education , Beijing 100029 China.|Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029 China.
authors: Fu-Jian
authors: FJ
authors: Xu
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D018014:Gene Transfer Techniques; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D005936:Glucans; C009109:pullulan
keywords: MRI; PGMA; cationic nanoparticle; gene delivery; pullulan