title: Diabetic Lactoferrin Deficient Mice Demonstrates Greater Susceptibility to Experimental Periodontal Disease.
abstract: The objective of this study is to detrmine whether alloxan-induced diabetic Lactoferrin knockout (LFKO-/-) mice are more susceptible to periodontal disease caused byAggregatibacter actinomycetemcomitanscompared to the diabetic wild-type (WT) mice. Diabetes was induced in mice by a single dose of alloxan (60 mg/kg) injected intravenously. Mice were categorized as diabetic when blood glucose levels >250 mg/dL were measured on the 7thday after the injection. Periodontal disease was experimentally induced byA. actinomycetemcomitansinfection in alloxan induced diabetic WT and LFKO-/-mice. Fasting blood glucose levels and body weight were monitored throughout the study. At the end of the 12thweek of infection, mice were sacrificed and bone loss among the groups was estimated by measuring the distance between cemento-enamel junction (CEJ) to the alveolar bone crest (ABC) at 12 sites on the molars.A. actinomycetemcomitansinfected mice groups developed more alveolar bone loss than sham-infected animals. Diabetic LFKO-/-infected mice exhibited significant bone loss (P<0.01) and a higher mean fasting blood glucose level (P<0.05) when compared to diabetic WT infected mice. No statistically significant difference in fasting blood glucose level was found between the infected and sham-infected groups. Peripheral blood analysis at the end of the 12thweek revealed a significant reduction in the platelet counts in LFKO-/-mice when compared to WT mice. Furthermore, diabetic LFKO-/-presented with lower counts than non-diabetic LFKO-/-mice (P<0.01). In conclusion, diabetic lactoferrin deficient mice are at a higher risk of developing periodontal infection induced byA. actinomycetemcomitanswhen compared to diabetic WTI mice.
authors: Rutgers School of Dental Medicine, Department of Oral Biology, Newark, New Jersey, USA.
authors: Waad
authors: W
authors: Alabdulmohsen
authors: Rutgers School of Dental Medicine, Department of Oral Biology, Newark, New Jersey, USA.
authors: Sonia D
authors: SD
authors: Rozario
authors: Rutgers School of Dental Medicine, Department of Oral Biology, Newark, New Jersey, USA.
authors: Kenneth
authors: K
authors: Markowitz
authors: Rutgers School of Dental Medicine, Department of Oral Biology, Newark, New Jersey, USA.
authors: Daniel H
authors: DH
authors: Fine
authors: Rutgers School of Dental Medicine, Department of Oral Biology, Newark, New Jersey, USA.
authors: Kabilan
authors: K
authors: Velliyagounder
keywords: Aggregatibacter actinomycetemcomitans; Diabetes; Lactoferrin; Lactoferrin knockout mice; Periodontal disease