title: ALTERATION OF OXIDATIVE METABOLISM AND IMMUNOLOGICAL PARAMETERS UNDER THE INFLUENCE OF UVA RADIATION IN WOMEN OF DIFFERENT AGES.
abstract: Purpose of study was to investigate the alteration of oxidative and immunological metabolism in blood of reproductive and menopausal age women after UVA irradiation. Women of two groups (1 - menopausal, 2 - reproductive age) were exposed to radiation with wave length 320-400 nm for 5-10 minutes per session in the solarium for the period of 3 months (6 days per month).. Parameters of oxidative metabolism - activity of red blood cells (RBC), antioxidant enzymes catalase (CAT) and superoxiddismutase (SOD) were studied by spectrophotometric method; reactive oxygen (O2-) and lipid (LOO/) free radicals content in whole blood were studied by Electron Paramagnetic Resonance (EPR) methods with use of specific spin-traps - 5,5-Dimethyl-1-Pyrroline-N-Oxide (DMPO) and α-phenyl-N-tert-butylnitrone (PBN) (SIGMA). Parameters of immunological metabolism - IFN-α, IL-2, IL-10 cytokines content in blood were studied by immunoenzymatic assay ELISA. The study protocol has been approved by the Ethical Committee of the Tbilisi State Medical University. After the course of UVA irradiation in menopausal women CAT activity increased by 20%, SOD - by 24%, and EPR signal of spin trapped lipoperoxide radicals was detected. No alterations in blood redox-balance were detected in women of reproductive age. Values of blood immunological parameters in menopausal women were not changed under the influence of UV radiation; in women of reproductive age IL-10 content increase by 93% (within a normal value range) was revealed. Research results have shown that UVA rays cause a particularly significant influence on the oxidative metabolism in the women of menopausal age. Increase of IL-10 blood levels in women of reproductive age (considering its imunosupressing activity) represents an additional risk of cancerogenesis. Based on the study results, we recommend avoiding UV (including UVA) radiation procedures to women of both reproductive and menopausal ages.
authors: Tbilisi State Medical University, Department of Dermatology and Venerology; Department of Medical Physics and Biophysics, Georgia.
authors: K
authors: K
authors: Berianidze
authors: Tbilisi State Medical University, Department of Dermatology and Venerology; Department of Medical Physics and Biophysics, Georgia.
authors: A
authors: A
authors: Katsitadze
authors: Tbilisi State Medical University, Department of Dermatology and Venerology; Department of Medical Physics and Biophysics, Georgia.
authors: T
authors: T
authors: Sanikidze
mesh_terms: D000328:Adult; D000367:Age Factors; D002374:Catalase / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008054:Lipid Peroxides / Q000097:blood*; D008875:Middle Aged; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000097:blood*; D013481:Superoxides / Q000097:blood*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D016207:Cytokines; D016898:Interferon-alpha; D007376:Interleukin-2; D008054:Lipid Peroxides; D013481:Superoxides; D016753:Interleukin-10; D002374:Catalase; D013482:Superoxide Dismutase