title: The Hot-Water Extract of Smilacis Chinae Rhizome Suppresses 2,4-Dinitrochlorobenzene and House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions in Mice.
abstract: Smilacis Chinae Rhizome (SCR) has been used as an oriental folk medicine for various biological activities. However, its effect on atopic dermatitis (AD) remains undetermined to date. We assessed the effect of orally administered hot-water extract of SCR on AD-like skin lesions in mice and its underlying mechanisms. AD-like murine model was prepared by repeated alternate application of house dust mite (Dermatophagoides farinae) extract (DFE) and 2,4-dinitrochlorobenzene (DNCB) for 4 weeks, topically to the ears. Daily oral administration of SCR for 3 and 4 weeks significantly reduced inflammatory ear thickening, with the effect being enhanced at the earlier start and longer period of administration. This effect was accompanied by a significant decrease in both Th2 and Th1 serum antibodies (total IgE, DFE-specific IgE, and IgG2a). Histological analysis showed that SCR markedly decreased the epidermal/dermal ear thickening and the dermal infiltration of inflammatory cells. Furthermore, SCR suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-17, IL-18, TSLP, and IFN-γ genes in the ear tissue. Taken together, our observations demonstrate that chronic oral administration of SCR exerts beneficial effect in mouse AD model, suggesting that SCR has the therapeutic potential as an orally active treatment of AD by modulating both Th1 and Th2 responses.
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Nam Yong
authors: NY
authors: Ki
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Eun-Ji
authors: EJ
authors: Park
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: In sung
authors: Is
authors: Sung
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Seul A
authors: SA
authors: Ju
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Kyoung Un
authors: KU
authors: Kim
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Mi Rae
authors: MR
authors: Kim
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Do Yeon
authors: DY
authors: Song
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Min-Ju
authors: MJ
authors: Lee
authors: Chemon Non-clinical Research Institute, Yangji, 17162, Korea.
authors: Hak-Soo
authors: HS
authors: Kim
authors: Chemon Non-clinical Research Institute, Yangji, 17162, Korea.
authors: Boo-Hyon
authors: BH
authors: Kang
authors: Industrial Medicine Department, Chungju Hospital, Konkuk Medical School, Konkuk University, Chungju, 27478, Korea.
authors: Hun-Jong
authors: HJ
authors: Chung
authors: Division of Sport Science, College of Science and Technology, Konkuk University, Chungju, 27478, Korea.
authors: Eun-Ju
authors: EJ
authors: Choi
authors: College of Pharmacy, Chung-Ang University, Seoul, 06974, Korea.
authors: Ki-Hun
authors: KH
authors: Yoon
authors: College of Pharmacy, Chung-Ang University, Seoul, 06974, Korea.
authors: Min Won
authors: MW
authors: Lee
authors: Novarex Co., Ltd, Seoul, 06626, Korea.
authors: Seongho
authors: S
authors: Yun
authors: Novarex Co., Ltd, Seoul, 06626, Korea.
authors: Bokkee
authors: B
authors: Min
authors: Novarex Co., Ltd, Seoul, 06626, Korea.
authors: Suk Hyung
authors: SH
authors: Kwon
authors: Department of Biomedical Chemistry, College of Biomedical and Health Sciences, Konkuk University, Chungju, 27478, Korea.
authors: Hwa-Sup
authors: HS
authors: Shin
mesh_terms: D000818:Animals; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy*; D040001:Dermatophagoides farinae / Q000276:immunology; D004137:Dinitrochlorobenzene / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007378:Interleukins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D032281:Smilax / Q000737:chemistry*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D004137:Dinitrochlorobenzene; D007074:Immunoglobulin G; D007378:Interleukins; D010936:Plant Extracts; D007073:Immunoglobulin E
keywords: DFE/DNCB; Smilacis Chinae Rhizome; Th1; Th2; atopic dermatitis; inflammation