title: Chicken sperm transcriptome profiling by microarray analysis.
abstract: It has been confirmed that mammalian sperm contain thousands of functional RNAs, and some of them have vital roles in fertilization and early embryonic development. Therefore, we attempted to characterize transcriptome of the sperm of fertile chickens using microarray analysis. Spermatozoal RNA was pooled from 10 fertile males and used for RNA preparation. Prior to performing the microarray, RNA quality was assessed using a bioanalyzer, and gDNA and somatic cell RNA contamination was assessed by CD4 and PTPRC gene amplification. The chicken sperm transcriptome was cross-examined by analysing sperm and testes RNA on a 4 × 44K chicken array, and results were verified by RT-PCR. Microarray analysis identified 21,639 predominantly nuclear-encoded transcripts in chicken sperm. The majority (66.55%) of the sperm transcripts were shared with the testes, while surprisingly, 33.45% transcripts were detected (raw signal intensity greater than 50) only in the sperm and not in the testes. The greatest proportion of up-regulated transcripts were responsible for signal transduction (63.20%) followed by embryonic development (56.76%) and cell structure (56.25%). Of the 20 most abundant transcripts, 18 remain uncharacterized, whereas the least abundant genes were mostly associated with the ribosome. These findings lay a foundation for more detailed investigations on sperm RNAs in chickens to identify sperm-based biomarkers for fertility.
authors: a Avian Physiology and Genetics Division, Sálim Ali Centre for Ornithology and Natural History, Anaikatty-641108, Coimbatore, India.
authors: R P
authors: RP
authors: Singh
authors: a Avian Physiology and Genetics Division, Sálim Ali Centre for Ornithology and Natural History, Anaikatty-641108, Coimbatore, India.
authors: C M
authors: CM
authors: Shafeeque
authors: b Central Avian Research Institute, Izatnagar, 243122, India.
authors: S K
authors: SK
authors: Sharma
authors: c Indian Veterinary Research Institute, Izatnagar, 243122, India.
authors: R
authors: R
authors: Singh
authors: b Central Avian Research Institute, Izatnagar, 243122, India.
authors: J
authors: J
authors: Mohan
authors: b Central Avian Research Institute, Izatnagar, 243122, India.
authors: K V H
authors: KV
authors: Sastry
authors: b Central Avian Research Institute, Izatnagar, 243122, India.
authors: V K
authors: VK
authors: Saxena
authors: a Avian Physiology and Genetics Division, Sálim Ali Centre for Ornithology and Natural History, Anaikatty-641108, Coimbatore, India.
authors: P A
authors: PA
authors: Azeez
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D020869:Gene Expression Profiling; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA; D013094:Spermatozoa / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D012313:RNA
keywords: ARN de sperme; biomarqueur pour la fécondité; développement embyronnaire; egg; embryonic development; fertility biomarker; fertilization; fécondation; sperm RNA; œuf