title: A novelty strategy for the fast analysis of sulfonamide antibiotics in fish tissue using magnetic separation with high-performance liquid chromatography-tandem mass spectrometry.
abstract: A simple, fast and low-cost extraction method with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) determination was developed on sulfonamide antibiotics (SAs) in fish tissue. Magnetic separation was first introduced into the rapid sample preparation procedure combined with acetonitrile extraction for the analysis of SAs. Partitioning was rapidly achieved between acetonitrile solution and solid matrix by applying an external magnetic field. Acetonitrile solution was collected and concentrated under a nitrogen stream. The residue was redissolved with 1‰ formic acid aqueous solution and defatted with n-hexane before analysis. The recoveries of SAs were in the range of 74.87-104.74%, with relative standard deviations <13%. The limits of quantification and the limits of detection for SAs ranged from 5.0 to 25.0 μg (kg-1) and from 2.5 to 10.0 μg (kg-1) , respectively. The presented extraction method proved to be a rapid method which only took 20 min for one sample preparation procedure. Copyright © 2016 John Wiley & Sons, Ltd.
authors: Chinese Academy of Fishery Sciences, Beijing, 100141, People's Republic of China.
authors: Jincheng
authors: J
authors: Li
authors: Chinese Academy of Fishery Sciences, Beijing, 100141, People's Republic of China.
authors: Huan
authors: H
authors: Liu
authors: Chinese Academy of Fishery Sciences, Beijing, 100141, People's Republic of China.
authors: Jing
authors: J
authors: Zhang
authors: Capital Normal University, Beijing, 100048, People's Republic of China.
authors: Yang
authors: Y
authors: Liu
authors: Chinese Academy of Fishery Sciences, Beijing, 100141, People's Republic of China.
authors: Lidong
authors: L
authors: Wu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005399:Fishes; D057230:Limit of Detection; D008280:Magnetics; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D013449:Sulfonamides
keywords: HPLC-MS/MS; fish tissue; magnetic separation; sulfonamide antibiotics