title: Purification and comparison of heat shock protein 90 (Hsp90) in Candida albicans isolates from Malaysian and Iranian patients and infected mice.
abstract: OBJECTIVE
  The purposes of this study were to purify and compare the concentration ratios of heat shock protein 90 (Hsp90) in clinical isolates of Candida albicans (C. albicans) obtained from Malaysian and Iranian patients and infected mice.
  
  
  METHODS
  Hsp90 was extracted using glass beads and ultracentrifugation from yeast cells and purified by ion exchange chromatography (DEAE-cellulose) and followed by affinity chromatography (hydroxyapatite). Purity of Hsp90 was controlled by SDS-PAGE and its identification was realized by immunoblotting test.
  
  
  RESULTS
  The graphs of ion exchange and affinity chromatography showed one peak in all C. albicans isolates obtained from both Malaysian and Iranian samples, infected mice and under high-thermal (42°C) and low-thermal (25°C) shock. In immunoblotting, the location of Hsp90 fragments was obtained around 47, 75 and 82kDa. The least average concentration ratios of Hsp90 were 0.350 and 0.240mg/g for Malaysian and Iranian isolates at 25°C, respectively, while the highest average concentration ratios of Hsp90 were 3.05 and 2.600mg/g for Malaysian and Iranian isolates at 42°C, respectively. There were differences in the ratio amount of Hsp90 between Malaysian isolates (1.01±0.07mg/g) and mice kidneys (1.23±0.28mg/g) as well as between Iranian isolates (0.70±0.19mg/g) and mice kidneys (1.00±0.28mg/g) (P<0.05).
  
  
  CONCLUSIONS
  The results showed differences in all situations tested including Iranian and Malaysian isolates, samples treated with temperatures (25°C or 42°C) and before and after infecting the mice (37°C), indicating higher virulent nature of this yeast species in high temperature in human and animal models.
authors: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: V
authors: V
authors: Khalili
authors: Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Imam Khomeini Street, 24th Aftab, Amol, Iran. Electronic address: hshokri@ausmt.ac.ir.
authors: H
authors: H
authors: Shokri
authors: Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors: A R
authors: AR
authors: Khosravi
authors: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: A
authors: A
authors: Akim
authors: Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors: S
authors: S
authors: Amri Saroukolaei
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002176:Candida albicans / Q000302:isolation & purification* / Q000378:metabolism*; D002177:Candidiasis / Q000382:microbiology* / Q000662:veterinary; D016022:Case-Control Studies; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007492:Iran; D008296:Malaysia; D008297:Male; D051379:Mice; D008875:Middle Aged; D012376:Rodent Diseases / Q000378:metabolism / Q000382:microbiology; D055815:Young Adult
chemical_list: D018841:HSP90 Heat-Shock Proteins
keywords: Candida albicans; Candidiasis; Candidose; Chromatographie; Chromatography; Heat shock protein; Malaysian and Iranian populations; Populations malaisiennes et iraniennes; Protéine de choc thermique