title: Long noncoding RNA-MEG3 is involved in diabetes mellitus-related microvascular dysfunction.
abstract: Microvascular dysfunction is an important characteristic of diabetic retinopathy. Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes. In this study, we investigated the role of lncRNA-MEG3 in diabetes-related microvascular dysfunction. We show that MEG3 expression level is significantly down-regulated in the retinas of STZ-induced diabetic mice, and endothelial cells upon high glucose and oxidative stress. MEG3 knockdown aggravates retinal vessel dysfunction in vivo, as shown by serious capillary degeneration, and increased microvascular leakage and inflammation. MEG3 knockdown also regulates retinal endothelial cell proliferation, migration, and tube formation in vitro. The role of MEG3 in endothelial cell function is mainly mediated by the activation of PI3k/Akt signaling. MEG3 up-regulation may serve as a therapeutic strategy for treating diabetes-related microvascular complications.
authors: Department of Health, Linyi People's Hospital, Shandong University, Shandong, China.
authors: Gui-Zhen
authors: GZ
authors: Qiu
authors: Department of Nursing, Linyi Oncosurgical Hospital, Shandong, China.
authors: Wei
authors: W
authors: Tian
authors: Department of Ophthalmology, Linyi People's Hospital, Shandong University, Shandong, China.
authors: Hai-Tao
authors: HT
authors: Fu
authors: Eye Institute of Xuzhou, Jiangsu, China. Electronic address: lcpcn@163.com.
authors: Chao-Peng
authors: CP
authors: Li
authors: Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address: liuban@126.com.
authors: Ban
authors: B
authors: Liu
mesh_terms: D000818:Animals; D003930:Diabetic Retinopathy / Q000378:metabolism* / Q000503:physiopathology*; D015536:Down-Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000503:physiopathology*; D062085:RNA, Long Noncoding / Q000378:metabolism*; D012171:Retinal Vessels / Q000503:physiopathology*
chemical_list: C422882:MEG3 non-coding RNA, mouse; D062085:RNA, Long Noncoding
keywords: Diabetic retinopathy; Long noncoding RNA; Microvascular dysfunction; PI3k/Akt signaling