title: Genetic divergence of tomato ringspot virus.
abstract: Tomato ringspot virus (ToRSV) has been detected in Chile, causing economically important diseases in a wide range of hosts. A ToRSV isolate was obtained from raspberry cv Heritage (Rasp-CL) showing leaf yellowing and stunting. The complete genome of Rasp-CL was sequenced by deep sequencing. The Rasp-CL RNA1 sequence shared 97.4 % nucleotide sequence identity with divergent RNA1 of isolate Rasp1-2014, while Rasp-CL RNA2 showed high divergence from all four isolates available in the database, sharing only 63.9-72.7 % nucleotide sequence identity. This difference was mainly based on the X4 coding region, which has been reported to be a high-variability region. Moreover, based on differences in the X4 region, three Rasp-CL RNA2 variants of different length were identified in the same host. One putative recombination event was identified between the Rasp-CL and GYV-2014 X4 genes. Phylogenetic analysis suggested that ToRSV isolates with currently available sequences form three distinct groups. Our results suggest that, for an accurate phylogenetic classification of ToRSV, it is necessary to obtain sequences of both RNAs. This is the first report of a complete ToRSV genome sequence from South America.
authors: Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Universidad de Chile, Santiago, Chile.
authors: Lucia
authors: L
authors: Rivera
authors: Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Universidad de Chile, Santiago, Chile.
authors: Alan
authors: A
authors: Zamorano
authors: Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Universidad de Chile, Santiago, Chile. nfiore@uchile.cl.
authors: Nicola
authors: http://orcid.org/0000-0001-6494-2257
authors: N
authors: Fiore
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002677:Chile; D016679:Genome, Viral / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016415:Sequence Alignment