title: Identification of Conserved and Diverse Metabolic Shifts during Rice Grain Development.
abstract: Seed development dedicates to reserve synthesis and accumulation and uncovering its genetic and biochemical mechanisms has been a major research focus. Although proteomic and transcriptomic analyses revealed dynamic changes of genes and enzymes involved, the information regarding concomitant metabolic changes is missing. Here we investigated the dynamic metabolic changes along the rice grain development of two japonica and two indica cultivars using non-targeted metabolomics approach, in which we successfully identified 214 metabolites. Statistical analyses revealed both cultivar and developmental stage dependent metabolic changes in rice grains. Generally, the stage specific metabolic kinetics corresponded well to the physiological status of the developing grains, and metabolic changes in developing rice grain are similar to those of dicot Arabidopsis and tomato at reserve accumulation stage but are different from those of dicots at seed desiccation stage. The remarkable difference in metabolite abundances between japonica and indica rice grain was observed at the reserve accumulation stage. Metabolite-metabolite correlation analysis uncovered potential new pathways for several metabolites. Taken together, this study uncovered both conserved and diverse development associated metabolic kinetics of rice grains, which facilitates further study to explore fundamental questions regarding the evolution of seed metabolic capabilities as well as their potential applications in crop improvement.
authors: Joint International Research Laboratory of Metabolic &Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors: Chaoyang
authors: C
authors: Hu
authors: Central Metabolism Group, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.
authors: Takayuki
authors: T
authors: Tohge
authors: Agilent Technology, Inc. Taipei, Taiwan.
authors: Shen-An
authors: SA
authors: Chan
authors: Agilent Technology, Inc. Shanghai, China.
authors: Yue
authors: Y
authors: Song
authors: Joint International Research Laboratory of Metabolic &Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.|Jiangxi Cancer Hospital, No. 519 East Beijing Road, Nanchang 330029, China.
authors: Jun
authors: J
authors: Rao
authors: Joint International Research Laboratory of Metabolic &Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors: Bo
authors: B
authors: Cui
authors: Joint International Research Laboratory of Metabolic &Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors: Hong
authors: H
authors: Lin
authors: Agilent Technology, Inc. Beijing 100000, China.
authors: Lei
authors: L
authors: Wang
authors: Central Metabolism Group, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.
authors: Alisdair R
authors: AR
authors: Fernie
authors: Joint International Research Laboratory of Metabolic &Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.
authors: Dabing
authors: D
authors: Zhang
authors: Joint International Research Laboratory of Metabolic &Developmental Sciences, SJTU-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minghan District, Shanghai 200240, China.|Shanghai Ruifeng Agro-biotechnology Co., Ltd, Room 108, No 233 Rushan Rd., Shanghai 200120, China.
authors: Jianxin
authors: J
authors: Shi
mesh_terms: D005075:Biological Evolution; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D002523:Edible Grain; D008401:Gas Chromatography-Mass Spectrometry; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D012275:Oryza / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism