title: Comparison of alkali treatments for efficient release of p-coumaric acid and enzymatic saccharification of sorghum pith.
abstract: Two separate temperature and time ranges were respectively conducted for optimizing release of p-coumaric acid and enzymatic saccharification of sorghum pith by NaOH pretreatment using response surface methodology. Two desirable pretreatment conditions were selected as follows: 37°C, 2% NaOH and 12h, and 100°C, 1.75% NaOH and 37min in the low and high temperature ranges, respectively. Under these conditions, the enzymatic glucose yields were 85.6% and 90.4% respectively, whereas p-coumaric acid yields were 95.1% and 98.1% respectively. The final recovery of esterified p-coumaric acid reached 82.8% and 87.4% respectively after further separation with HP-20 resin. Interestingly, strong linear correlations exist between p-coumaric acid release with glucan saccharification yield and lignin dissolution. These results indicate that sorghum pith could be an attractive source for natural p-coumaric acid and efficient release of p-coumaric acid and enzymatic saccharification of sorghum pith can be achieved by mild NaOH pretreatment.
authors: College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
authors: Kankan
authors: K
authors: Jiang
authors: College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
authors: Lulu
authors: L
authors: Li
authors: College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
authors: Liangkun
authors: L
authors: Long
authors: College of Chemical Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China. Electronic address: dshaojun@hotmail.com.
authors: Shaojun
authors: S
authors: Ding
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D003373:Coumaric Acids / Q000302:isolation & purification*; D006868:Hydrolysis; D008031:Lignin / Q000302:isolation & purification; D008962:Models, Theoretical; D011422:Propionates; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000494:pharmacology; D045868:Sorghum / Q000737:chemistry* / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D014866:Waste Products; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D000468:Alkalies; D003373:Coumaric Acids; D011422:Propionates; D014866:Waste Products; D012972:Sodium Hydroxide; D008031:Lignin; D001617:beta-Glucosidase; C495469:p-coumaric acid
keywords: Enzymatic hydrolysis; Linear correlation; NaOH treatment; Sorghum pith; p-Coumaric acid