title: Evaluation of two methods for direct detection of Fusarium spp. in water.
abstract: Fusarium is a waterborne fungus that causes severe infections especially in patients with prolonged neutropenia. Traditionally, the detection of Fusarium in water is done by culturing which is difficult and time consuming. A faster method is necessary to prevent exposure of susceptible patients to contaminated water. The objective of this study was to develop a molecular technique for direct detection of Fusarium in water. A direct DNA extraction method from water was developed and coupled to a genus-specific PCR, to detect 3 species of Fusarium (verticillioides, oxysporum and solani). The detection limits were 10 cells/L and 1 cell/L for the molecular and culture methods, respectively. To our knowledge, this is the first method developed to detect Fusarium directly from water.
authors: Department of Infectious Diseases, Faculty of Medicine, University of São Paulo, Brazil; LIM 54, Institute of Tropical Medicine of the University of São Paulo, Brazil. Electronic address: mariana.ggraca@gmail.com.
authors: Mariana G
authors: MG
authors: Graça
authors: Department of Pathology, Faculty of Medicine of ABC, Brazil. Electronic address: inneke@usp.br.
authors: Inneke M
authors: IM
authors: van der Heijden
authors: Department of Infectious Diseases, Faculty of Medicine, University of São Paulo, Brazil; LIM 54, Institute of Tropical Medicine of the University of São Paulo, Brazil. Electronic address: lauro.neto@hc.fm.usp.br.
authors: Lauro
authors: L
authors: Perdigão
authors: Laboratory of Pathogenic Dimorphic Fungi - Institute of Biomedical Sciences, University of São Paulo, Brazil; Laboratory of Medical Investigation 53, Faculty of Medicine, University of São Paulo, Brazil. Electronic address: cleisont@yahoo.com.br.
authors: Cleison
authors: C
authors: Taira
authors: Department of Infectious Diseases, Faculty of Medicine, University of São Paulo, Brazil; LIM 54, Institute of Tropical Medicine of the University of São Paulo, Brazil. Electronic address: costasilviaf@ig.com.br.
authors: Silvia F
authors: SF
authors: Costa
authors: Department of Infectious Diseases, Faculty of Medicine, University of São Paulo, Brazil; LIM 54, Institute of Tropical Medicine of the University of São Paulo, Brazil. Electronic address: gcih.adm@hc.fm.usp.br.
authors: Anna S
authors: AS
authors: Levin
mesh_terms: D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal
keywords: Cultures; Environment; Fusarium spp.; Polymerase chain reaction; Water