title: Alteration of cytokine profile following hemorrhagic shock.
abstract: Hemorrhage is one of the leading causes of death in patients with trauma. We recently demonstrated that resveratrol can improve cardiac function and prolong life following severe hemorrhagic injury (HI) in a rat model. The present work is focused on determining changes in NF-κB dependent gene expression in the heart and the systemic cytokine milieu following HI and the effect of resveratrol treatment. The results indicate an increase in phosphorylated NF-κB in the heart with a concomitant increase in the expression of NF-κB dependent genes following HI. There was also a significant increase of systemic cytokine levels, both pro and anti-inflammatory, following HI and resolution when treated with resveratrol. This study demonstrates the potential role NF-κB has in the physiological response to HI and the effectiveness of resveratrol in reducing immune activation.
authors: Georgia Regents University, Augusta, GA, United States.
authors: Sumin
authors: S
authors: Lu
authors: Georgia Regents University, Augusta, GA, United States.
authors: Alex
authors: A
authors: Aguilar
authors: Georgia Regents University, Augusta, GA, United States.
authors: Kumar
authors: K
authors: Subramani
authors: Georgia Regents University, Augusta, GA, United States.
authors: Ninu
authors: N
authors: Poulose
authors: Georgia Regents University, Augusta, GA, United States.
authors: Ahmar
authors: A
authors: Ayub
authors: Georgia Regents University, Augusta, GA, United States. Electronic address: RRaju@GRU.edu.
authors: Raghavan
authors: R
authors: Raju
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D015153:Blotting, Western; D054405:Chemokine CCL3 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000627:therapeutic use*; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D054405:Chemokine CCL3; D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D013267:Stilbenes; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D000077185:Resveratrol
keywords: Hemorrhage; Inflammation; NFκB; Shock; Trauma