title: A novel paramyxean parasite, Marteilia octospora n. sp. (Cercozoa) infecting the Grooved Razor Shell clam Solen marginatus from Galicia (NW Spain).
abstract: Protozoan parasites of the genus Marteilia have been detected in marine bivalves and other invertebrates around the world, associated in some cases with mass mortalities. The present paper reports the characterization of the Marteilia sp. protozoan infecting the digestive gland of the Grooved Razor Shell clam (Solen marginatus) from Galicia (NW Spain), proposing a novel species in the genus: Marteilia octospora n. sp. Morphological and molecular techniques were used for the description of this parasite. Tissue imprints were essential in the study to confirm the presence of 8 spores per sporangium, a number never reported in other species from this genus. An ultrastructural study revealed that the size and number of dense granules, free in the mature sporangia, were quite different from granules in other Marteilia spp. Another morphological difference is the absence of a layer of concentric membranes found surrounding the mature spore in other species. In addition, concentric membranous structures observed in the different stages of the parasite have never been mentioned in other species of genus Marteilia. Moreover, molecular analysis of the rDNA intergenic spacer (IGS) and the internal transcribed spacer (ITS-1) showed differences with the sequences available for other Marteilia spp.
authors: Centro de Investigacións Mariñas (CIMA), Consellería do Mar, Xunta de Galicia, Pedras do Corón, P.O. Box 13, 36620 Vilanova de Arousa, Pontevedra, Spain.
authors: Maite
authors: M
authors: Ruiz
authors: Centro de Investigacións Mariñas (CIMA), Consellería do Mar, Xunta de Galicia, Pedras do Corón, P.O. Box 13, 36620 Vilanova de Arousa, Pontevedra, Spain.
authors: Carmen
authors: C
authors: López
authors: Departamento I+D+i, Consello Regulador do Mexillón de Galicia, Avenida da Mariña, 25, 36600 Vilagarcía de Arousa, Pontevedra, Spain.
authors: Ren-Shiang
authors: RS
authors: Lee
authors: Centro de Investigacións Mariñas (CIMA), Consellería do Mar, Xunta de Galicia, Pedras do Corón, P.O. Box 13, 36620 Vilanova de Arousa, Pontevedra, Spain.
authors: Rosana
authors: R
authors: Rodríguez
authors: Instituto Tecnolóxico para o Control do Medio Mariño de Galicia (INTECMAR), Consellería do Mar, Xunta de Galicia, Peirao de Vilaxoán s/n, 36611 Vilagarcía de Arousa, Pontevedra, Spain. Electronic address: sdarriba@intecmar.org.
authors: Susana
authors: S
authors: Darriba
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000469:parasitology* / Q000648:ultrastructure; D056907:Cercozoa / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D013030:Spain; D033761:Spores, Protozoan / Q000648:ultrastructure
chemical_list: D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S
keywords: Imprints; Marteilia; Paramyxean; Solen marginatus; Ultrastructure; rDNA SSU