title: Overexpression of Kinesin Associated Protein 3 (KIFAP3) in Breast Cancer.
abstract: Gene expression profiling studies on breast cancer have generated catalogs of differentially expressed genes. However, many of these genes have not been investigated for their expression at the protein level. It is possible to systematically evaluate such genes in a high-throughput fashion for their overexpression at the protein level using breast cancer tissue microarrays. Our strategy involved integration of information from publicly available repositories of gene expression to prepare a list of genes upregulated at the mRNA level in breast cancer followed by curation of the published literature to identify those genes that were not tested for overexpression at the protein level. We identified Kinesin Associated Protein 3 (KIFAP3) as one such molecule for further validation at the protein level. Immunohistochemical labeling of KIFAP3 using breast cancer tissue microarrays revealed overexpression of KIFAP3 protein in 84% (240/285) of breast cancers indicating the utility of our integrated approach of combining computational analysis with experimental biology.
authors: Institute of Bioinformatics, International Tech Park, Bangalore-560 066, India; Department of Biotechnology, Kuvempu University, Shankaraghatta-577451, India.
authors: Deepthi
authors: D
authors: Telikicherla
authors: Institute of Bioinformatics, International Tech Park, Bangalore-560 066, India; Department of Pathology, Raja Rajeshwari Medical College and Hospital, Bangalore-560074, India; Manipal University, Madhav Nagar, Manipal-576104, India.
authors: Jagadeesha
authors: J
authors: Maharudraiah
authors: Institute of Bioinformatics, International Tech Park, Bangalore-560 066, India; Rajiv Gandhi University of Health Sciences, Bangalore-560041, India; Department of Pathology, Kidwai Memorial Institute of Oncology, Bangalore-560029, India.
authors: Harsh
authors: H
authors: Pawar
authors: Institute of Bioinformatics, International Tech Park, Bangalore-560 066, India.
authors: Arivusudar
authors: A
authors: Marimuthu
authors: Institute of Bioinformatics, International Tech Park, Bangalore-560 066, India.
authors: Manoj Kumar
authors: MK
authors: Kashyap
authors: Department of Biotechnology, Kuvempu University, Shankaraghatta-577451, India.
authors: Y L
authors: YL
authors: Ramachandra
authors: Department of Pathology, Universidad de La Frontera, Temuco, Chile.
authors: Juan Carlos
authors: JC
authors: Roa
authors: McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Akhilesh
authors: A
authors: Pandey
keywords: Breast neoplasms; Immunohistochemistry; KAP3; SMAP; SmgGDS associated protein