title: Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses.
abstract: A restriction fragment length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses (GPV and MDPV) was developed based on comparison of the NS gene of GPV and MDPV. Both GPV and MDPV genomic DNA can be amplified with 641 bp using the specific PCR primers. The PCR fragments can be cut into 463 bp and 178 bp only in the case of MDPV-derived PCR products, whereas the GPV-derived PCR products cannot. The method established in this study can be used to differentiate GPV and MDPV with high specificity and precision, by using a direct PCR kit and QuickCut enzyme, as quickly as conventional PCR.
authors: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, China.
authors: Chun-He
authors: CH
authors: Wan
authors: Hong-Mei
authors: HM
authors: Chen
authors: Qiu-Ling
authors: QL
authors: Fu
authors: Shao-Hua
authors: SH
authors: Shi
authors: Guang-Hua
authors: GH
authors: Fu
authors: Long-Fei
authors: LF
authors: Cheng
authors: Cui-Teng
authors: CT
authors: Chen
authors: Yu
authors: Y
authors: Huang
authors: Kai-Hui
authors: KH
authors: Hu
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D004372:Ducks / Q000821:virology*; D005777:Geese / Q000821:virology*; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D011201:Poultry Diseases / Q000821:virology*