title: Distinct molecular signatures of mild extrinsic and intrinsic atopic dermatitis.
abstract: Atopic dermatitis (AD) is a common inflammatory skin disease with underlying defects in epidermal function and immune responses. In this study, we used microarray analysis to investigate differences in gene expression in lesional skin from patients with mild extrinsic or intrinsic AD compared to skin from healthy controls and from lesional psoriasis skin. The primary aim was to identify differentially expressed genes involved in skin barrier formation and inflammation, and to compare our results with those reported for patients with moderate and severe AD. In contrast to severe AD, expression of the majority of genes associated with skin barrier formation was unchanged or upregulated in patients with mild AD compared to normal healthy skin. Among these, no significant differences in the expression of filaggrin (FLG) and loricrin at both mRNA and protein level were found in lesional skin from patients with mild AD, despite the presence of heterozygous FLG mutations in the majority of patients with mild extrinsic AD. Several inflammation-associated genes such as S100A9, MMP12, CXCL10 and CCL18 were highly expressed in lesional skin from patients with mild psoriasis and were also increased in patients with mild extrinsic and intrinsic AD similar to previous reports for severe AD. Interestingly, expression of genes involved in inflammatory responses in intrinsic AD resembled that of psoriasis more than that of extrinsic AD. Overall, differences in expression of inflammation-associated genes found among patients with mild intrinsic and extrinsic AD correlated with previous findings for patients with severe intrinsic and extrinsic AD.
authors: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.|Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.|LEO Pharma A/S, Ballerup, Denmark.
authors: Britta C
authors: BC
authors: Martel
authors: LEO Pharma A/S, Ballerup, Denmark.
authors: Thomas
authors: T
authors: Litman
authors: LEO Pharma A/S, Ballerup, Denmark.
authors: Andreas
authors: A
authors: Hald
authors: LEO Pharma A/S, Ballerup, Denmark.
authors: Hanne
authors: H
authors: Norsgaard
authors: LEO Pharma A/S, Ballerup, Denmark.
authors: Paola
authors: P
authors: Lovato
authors: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors: Beatrice
authors: B
authors: Dyring-Andersen
authors: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors: Lone
authors: L
authors: Skov
authors: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors: Kristian
authors: K
authors: Thestrup-Pedersen
authors: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors: Søren
authors: S
authors: Skov
authors: LEO Pharma A/S, Ballerup, Denmark.
authors: Kresten
authors: K
authors: Skak
authors: Department of Dermato-Allergology, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
authors: Lars K
authors: LK
authors: Poulsen
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000145:classification / Q000378:metabolism* / Q000473:pathology; D000091344:Filaggrin Proteins; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D011565:Psoriasis / Q000378:metabolism*; D012720:Severity of Illness Index; D012867:Skin / Q000378:metabolism / Q000473:pathology; D055815:Young Adult
keywords: epidermal differentiation complex; inflammation; microarray; psoriasis; skin barrier