title: [THE COMPARISON OF TECHNIQUES OF ELECTROPHORESIS, IMMUNE TURBIDYNAMIC MEASUREMENT AND PHENOTYPING OF ALPHA-1-ANTITRYPSIN FOR DIAGNOSTIC OF ALPHA-1-ANTITRYPSIN INSUFFICIENCY].
abstract: The qualitative and quantitative deficiency of alpha-1-antitrypsin (A1AT) is an inherited factor of susceptibility to a number of conditions including chronic obstructive disease of lungs and primary emphysema and liver affection. The study was carried out to evaluate analysis of alpha-1-fraction (A1F) using zonal electrophoresis technique for detecting patients with alpha-1-antitrypsin insufficiency (A1ATI). The patients with decreased (group I) and normal (group II) A1F on proteinogram. The electrophoresis was applied using the system of capillary electrophoresis Capillaris-2 Flex Piercing (Sebia, France) and also system for electrophoresis in agarose gel SAS-1/SAS-2 (Helena Biosciences, Great Britain). The commercial kit Sentinel diagnostics (Italy) and biochemical analyzer A15 (Biosystems, Spain) were used for quantitative detecting of A1AT The study results demonstrated that decreasing of A1F on proteinogram correlated with lessening of concentration of A1ATI in blood serum and with presence of its pathological phenotype. The average values of concentration of A1AT in group I and group II made up to 1148 and 1738 mg/I correspondingly. The total rate of pathological phenotypes made up to 76% (19/25) in group I that reliably differed from indicators in group II--7.1% (2/28). Thereby, electrophoresis of proteins of blood serum can be sufficiently informative for primary selection of patients requiring examination for presence of A1ATI.
authors: M Yu
authors: MY
authors: Pervakova
authors: V L
authors: VL
authors: Emanuel
authors: E A
authors: EA
authors: Surkova
authors: A V
authors: AV
authors: Mazing
authors: S V
authors: SV
authors: Lapin
authors: I S
authors: IS
authors: Kovaleva
authors: S N
authors: SN
authors: Sysoeva
mesh_terms: D001797:Blood Protein Electrophoresis / Q000379:methods*; D006801:Humans; D010641:Phenotype; D000515:alpha 1-Antitrypsin / Q000097:blood* / Q000737:chemistry / Q000235:genetics; D019896:alpha 1-Antitrypsin Deficiency / Q000097:blood*
chemical_list: D000515:alpha 1-Antitrypsin