title: The Anti-Atherosclerotic Effect of Naringin Is Associated with Reduced Expressions of Cell Adhesion Molecules and Chemokines through NF-κB Pathway.
abstract: Naringin has been reported to have an anti-atherosclerosis effect but the underlying mechanism is not fully understood. The aim of this study is to investigate the impact of naringin on the TNF-α-induced expressions of cell adhesion molecules, chemokines and NF-κB signaling pathway in human umbilical vein endothelial cells (HUVECs). The experiments revealed that naringin, at concentrations without cytotoxicity, dose-dependently inhibited the adhesion of THP-1 monocytes to the TNF-α-stimulated HUVECs. The TNF-α-induced expressions of cell adhesion molecules, including VCAM-1, ICAM-1 and E-selectin, at both the mRNA and protein levels, were significantly suppressed by naringin in a dose dependent manner. In addition, the TNF-α-induced mRNA and protein levels of chemokines, including fractalkine/CX3CL1, MCP-1 and RANTES, were also reduced by naringin. Naringin significantly inhibited TNF-α-induced nuclear translocation of NF-κB, which resulted from the inhibited phosphorylation of IKKα/β, IκB-α and NF-κB. Altogether, we proposed that naringin modulated TNF-α-induced expressions of cell adhesion molecules and chemokines through the inhibition of TNF-α-induced activation of IKK/NF-κB signaling pathway to exert the anti-atherosclerotic effect.
authors: Department of Chinese Medicine and Mitochondrial Research Unit, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Da-Pei Road, Niao-Sung District, Kaohsiung 83301, Taiwan. melaopin@gmail.com.
authors: Tun-Pin
authors: TP
authors: Hsueh
authors: Department of Chinese Medicine and Mitochondrial Research Unit, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Da-Pei Road, Niao-Sung District, Kaohsiung 83301, Taiwan. r12042@cgmh.org.tw.
authors: Jer-Ming
authors: JM
authors: Sheen
authors: Graduate Institute of Clinical Medical Sciences, Chang Gung University, No. 259, Wen-Hua 1st Road, Kwei-Shan, Tao-Yuan 33302, Taiwan. jonghwei@mail.cgu.edu.tw.
authors: Jong-Hwei S
authors: JH
authors: Pang
authors: Department of Chinese Medicine and Mitochondrial Research Unit, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Da-Pei Road, Niao-Sung District, Kaohsiung 83301, Taiwan. bikw@adm.cgmh.org.tw.
authors: Kuo-Wei
authors: KW
authors: Bi
authors: Division of General Surgery, Ministry of Health and Welfare Pingtung Hospital, No. 270, Ziyou Road, Pingtung City, Pingtung County 900, Taiwan. hcj5311@yahoo.com.tw.
authors: Chao-Chun
authors: CC
authors: Huang
authors: Department of Chinese Medicine and Mitochondrial Research Unit, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Da-Pei Road, Niao-Sung District, Kaohsiung 83301, Taiwan. b8606059@tmu.edu.tw.
authors: Hsiao-Ting
authors: HT
authors: Wu
authors: Department of Chinese Medicine and Mitochondrial Research Unit, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, No. 123, Da-Pei Road, Niao-Sung District, Kaohsiung 83301, Taiwan. sheng.teng@yahoo.com.|School of Chinese Medicine, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan. sheng.teng@yahoo.com.
authors: Sheng-Teng
authors: ST
authors: Huang
mesh_terms: D021581:Active Transport, Cell Nucleus; D050197:Atherosclerosis / Q000188:drug therapy; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D018920:Coculture Techniques; D044950:Flavanones / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D015815:Cell Adhesion Molecules; D018925:Chemokines; D044950:Flavanones; D016328:NF-kappa B; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; C005274:naringin
keywords: NF-κB; TNF-α; atherosclerosis; chemokines; naringin