title: Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo.
abstract: Porous tantalum metal with low elastic modulus is similar to cancellous bone. Reticulated vitreous carbon (RVC) can provide three-dimensional pore structure and serves as the ideal scaffold of tantalum coating. In this study, the biocompatibility of domestic porous tantalum was first successfully tested with bone marrow stromal stem cells (BMSCs) in vitro and for bone tissue repair in vivo. We evaluated cytotoxicity of RVC scaffold and tantalum coating using BMSCs. The morphology, adhesion, and proliferation of BMSCs were observed via laser scanning confocal microscope and scanning electron microscopy. In addition, porous tantalum rods with or without autologous BMSCs were implanted on hind legs in dogs, respectively. The osteogenic potential was observed by hard tissue slice examination. At three weeks and six weeks following implantation, new osteoblasts and new bone were observed at the tantalum-host bone interface and pores. At 12 weeks postporous tantalum with autologous BMSCs implantation, regenerated trabecular equivalent to mature bone was found in the pore of tantalum rods. Our results suggested that domestic porous tantalum had excellent biocompatibility and could promote new bone formation in vivo. Meanwhile, the osteogenesis of porous tantalum associated with autologous BMSCs was more excellent than only tantalum implantation. Future clinical studies are warranted to verify the clinical efficacy of combined implantation of this domestic porous tantalum associated with autologous BMSCs implantation and compare their efficacy with conventional autologous bone grafting carrying blood vessel in patients needing bone repairing.
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Xiaowei
authors: X
authors: Wei
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China zhaodewei2000@163.com.
authors: Dewei
authors: D
authors: Zhao
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Benjie
authors: B
authors: Wang
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Wei
authors: W
authors: Wang
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Kai
authors: K
authors: Kang
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Hui
authors: H
authors: Xie
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Baoyi
authors: B
authors: Liu
authors: Department of Orthopaedic Laboratory, Zhongshan Hospital of Dalian University, Dalian 116001, China.
authors: Xiuzhi
authors: X
authors: Zhang
authors: Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China.
authors: Jinsong
authors: J
authors: Zhang
authors: Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China.
authors: Zhenming
authors: Z
authors: Yang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000378:metabolism*; D001853:Bone Marrow; D001861:Bone Regeneration; D002244:Carbon / Q000009:adverse effects / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D006801:Humans; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D013635:Tantalum / Q000009:adverse effects / Q000378:metabolism*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D013635:Tantalum; D002244:Carbon
keywords: Bone marrow stromal stem cells; biocompatibility; bone tissue engineering; osteogenesis; porous tantalum