title: Effect of Nitric Oxide on the Expression of Matrix Metalloproteinase and Its Association with Migration of Cultured Trabecular Meshwork Cells.
abstract: OBJECTIVE
  To determine the effect of exogenous nitric oxide (NO) on the migration of trabecular meshwork (TM) cells and its association with expression of matrix metalloproteinases (MMPs).
  
  
  METHODS
  Primary human TM cells treated with 1 or 10 µM S-nitroso-N-acetyl-penicillamine (SNAP) and examined for changes in adherence. TM cells were seeded onto transwell culture inserts, and changes in their migratory activity were quantified. Reverse transcription polymerase chain reaction was performed to determine the relative changes in mRNA expression of MMPs and tissue inhibitor of metalloproteinases (TIMPs).
  
  
  RESULTS
  Treatment with SNAP did not significantly suppress TM cell adhesion or migration (p > 0.05). Treatment of TM cells with 10 µM SNAP decreased expression of MMP-2 and increased expression of membrane type MMP-1 and TIMP-2. Treatment with interleukin-1α triggered MMP-3 expression but did not exert significant effects on MMP-3 activation in response to SNAP.
  
  
  CONCLUSIONS
  These data suggest that NO revealed no significant effect on the migration of TM cells because NO decreased MMP-2 and increased TIMP-2 expression. Although expression of certain MMPs and TIMPs change in response to NO donors, NO may modulate trabecular outflow by changing the cellular production of extracellular matrix without having a significant effect on the migration of TM cells.
authors: Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea.
authors: Jae Woo
authors: JW
authors: Kim
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics*; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
chemical_list: D017931:DNA Primers; D020030:Nitric Oxide Donors; D012333:RNA, Messenger; C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2; D026423:S-Nitroso-N-Acetylpenicillamine; D020782:Matrix Metalloproteinases
keywords: Matrix metalloproteinase; Migration; Nitric oxide; Trabecular meshwork cells