title: Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions.
abstract: Objective:Compare the efficacy of light-emitting diode (LED) and therapeutic ultrasound (TUS), combined with a semipermeable dressing (D), at forming collagen in skin lesions by morphometry and Fourier transform infrared spectroscopy (FT-IR).Materials and Methods:Surgical skin wounds (2.5 cm) were created on 84 male Wistar rats divided into four groups (n=21): Group I (Control), Group II (LED), Group III (LED+D), and Group IV (US+D). On days 7, 14, and 21, the tissue samples were removed and divided into two pieces, one was used for histological examination (collagen) and the other for FT-IR.Results:The histomorphometric analysis showed no significant differences among groups for collagen deposition at 7 days. However, at 14 days, more deposition of collagen was noted in the groups LED (p<0.05) and LED+D (p<0.001) than in the control. At 21 days, the groups LED, LED+D, and US+D presented significantly greater deposition of collagen when compared with the control. The FT-IR spectra, at 14 days, LED+D had greater amounts of type I collagen, a better organization of fibers, and greater difference of mean separation between the groups, not observed at 7 and 21 days.Innovation:The histomorphometric and FT-IR analyses suggest that the association of semipermeable dressing to LED therapy and to TUS modulates biological events, increasing fibroblast/collagen response and accelerating dermal maturation.Conclusion:The histomorphometric and FT-IR analyses showed that LED therapy is more efficacious than TUS, when combined with a semipermeable dressing, and induced the collagen production in skin lesions.
authors: Universidade do Vale do Paraíba, UNIVAP, Teresina, São Paulo, Brazil.; Universidade Estadual do Piauí, UESPI, Teresina, Piauí, Brazil.; Faculdade Integral Diferencial, FACID, Teresina, Piauí, Brazil.
authors: Veruska Cronemberger
authors: VC
authors: Nogueira
authors: Universidade do Vale do Paraíba , UNIVAP, Teresina, São Paulo, Brazil .
authors: Leandro
authors: L
authors: Raniero
authors: Universidade do Vale do Paraíba , UNIVAP, Teresina, São Paulo, Brazil .
authors: Guilherme Bueno
authors: GB
authors: Costa
authors: Universidade do Vale do Paraíba, UNIVAP, Teresina, São Paulo, Brazil.; Universidade Estadual do Piauí, UESPI, Teresina, Piauí, Brazil.; Faculdade Integral Diferencial, FACID, Teresina, Piauí, Brazil.
authors: Nayana Pinheiro Machado
authors: NP
authors: de Freitas Coelho
authors: Universidade Estadual do Piauí , UESPI, Teresina, Piauí, Brazil .
authors: Fernando Cronemberger
authors: FC
authors: Miranda
authors: Universidade do Vale do Paraíba , UNIVAP, Teresina, São Paulo, Brazil .
authors: Emília Ângela Loschiavo
authors: EÂ
authors: Arisawa