title: Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera.
abstract: Haemorrhagic septicaemia caused by Pasteurella multocida is a major epizootic disease in cattle and buffaloes in developing countries with high morbidity and mortality rate. In the present study, a total of 88 P. multocida isolates were isolated from 256 nasopharyngeal swabs and lung tissues samples (34.4%) during the period from January, 2013 to March, 2014 from different governorates located in Egypt. Dead calves showed the highest percentage of P. multocida isolation followed by the emergency slaughtered calves, diseased calves then apparently healthy ones. These isolates were confirmed as P. multocida microscopically, biochemically by traditional tests and by API 20E commercial kit then by PCR. The percentages of positive serum samples using somatic antigen and micro-agglutination test at 1/1280 diluted serum were 10%, 54.49% and 0% in apparently healthy, diseased and emergency slaughtered samples, respectively whereas, the percentages using capsular antigen and indirect haemagglutination test were 40%, 60.89% and 60% in apparently healthy, diseased and emergency slaughtered samples, respectively. The ELISA showed the highest sensitivity for diagnosing P. multocida in apparently healthy, diseased and emergency slaughtered animals with percentages of 42%; 92.9% and 80%, respectively. The obtained results revealed that the ELISA using capsular antigen of P. multocida is a more sensitive and specific serological test for diagnosis of haemorrhagic septicaemia.
authors: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P. O. Box 2446, Cairo 14242, Giza, Egypt.
authors: Jakeen K
authors: JK
authors: El-Jakee
authors: Bacteriology Department, Animal Health Research Institute, P. O. Box 2440, Giza, Dokki, Egypt.
authors: Samah Said
authors: SS
authors: Ali
authors: Bacteriology Department, Animal Health Research Institute, P. O. Box 2440, Giza, Dokki, Egypt.
authors: Soumaya Ahmed
authors: SA
authors: El-Shafii
authors: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P. O. Box 2446, Cairo 14242, Giza, Egypt; Department of Health Science, College of Applied Studies and Community Service, King Saud University, P. O. Box 22459, Riyadh 11495, Saudi Arabia.
authors: Ashgan M
authors: AM
authors: Hessain
authors: Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh, Saudi Arabia.
authors: Abdullah A
authors: AA
authors: Al-Arfaj
authors: Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, P. O. Box 2446, Cairo 14242, Giza, Egypt; Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh, Saudi Arabia.
authors: Moussa I
authors: MI
authors: Mohamed
keywords: ELISA; HAT; Haemorrhagic septicaemia; IHAT; Serodiagnosis