title: Biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow-through systems.
abstract: We demonstrated the possibility of long-term and efficient application of a biosensitive element (BE) in the form of Photobacterium phosphoreum photobacteria immobilized in poly(vinyl alcohol) (PVA) cryogel for detecting various ecotoxicants (Zn(2) (+) , Cu(2) (+) , Hg(2) (+) , Pb(2) (+) , 2,4-dichlorophenoxyacetic acid, 2,6-dimethylphenol, pentachlorophenol, coumaphos, malathion, chlorpyrifos and methyl parathion) in flow-through media. The range of detectable concentrations of ecotoxicants was determined at 1 × 10(-8) to 1 × 10(-4) M for heavy metal ions and at 1 × 10(-8) to 1 × 10(-5) M for phenol derivatives and organophosphorus pesticides. Immobilized cells of photobacteria quantitatively reacted with these ecotoxicants; cell sensitivity exhibited no flow rate dependence in the range from 45 to 180 mL/h. At a constant concentration of ecotoxicant in the flow, the bioluminescence quenching profile of immobilized cells demonstrated an integral response. The BE could remain in a flow-through medium for at least 10 days while retaining 95% of luminescent activity in the absence of ecotoxicants. The BE tested in this work was demonstrated to have a long shelf life (> 60 weeks) at -80°C without changes in the baseline level of bioluminescence. Copyright © 2016 John Wiley & Sons, Ltd.
authors: Lomonosov Moscow State University, Moscow, Russia.
authors: Elena N
authors: EN
authors: Efremenko
authors: Lomonosov Moscow State University, Moscow, Russia.
authors: Olga V
authors: OV
authors: Maslova
authors: Lomonosov Moscow State University, Moscow, Russia.
authors: Alexander V
authors: AV
authors: Kholstov
authors: Lomonosov Moscow State University, Moscow, Russia.
authors: Olga V
authors: OV
authors: Senko
authors: Lomonosov Moscow State University, Moscow, Russia.
authors: Anvar D
authors: AD
authors: Ismailov
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis; D015374:Biosensing Techniques; D018914:Cells, Immobilized / Q000737:chemistry*; D004390:Chlorpyrifos / Q000032:analysis; D003372:Coumaphos / Q000032:analysis; D017022:Flow Injection Analysis; D049449:Luminescence; D008294:Malathion / Q000032:analysis; D019216:Metals, Heavy / Q000032:analysis; D008743:Methyl Parathion / Q000032:analysis; D010776:Photobacterium / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014992:Xylenes / Q000032:analysis
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D014992:Xylenes; D015084:2,4-Dichlorophenoxyacetic Acid; D008743:Methyl Parathion; C036531:2,6-xylenol; D004390:Chlorpyrifos; D003372:Coumaphos; D008294:Malathion
keywords: bioluminescence; biosensitive element; ecotoxicants; flow-through systems; immobilized cells