title: Setdb1, a novel interactor of ΔNp63, is involved in breast tumorigenesis.
abstract: ΔNp63 has been recently involved in self-renewal potential of breast cancer stem cells. Although the p63 transcriptional profile has been extensively characterized, our knowledge of the p63-binding partners potentially involved in the regulation of breast tumour progression is limited. Here, we performed the yeast two hybrid approach to identify p63α interactors involved in breast tumorigenesis and we found that SETDB1, a histone lysine methyl transferases, interacts with ΔNp63α and that this interaction contributes to p63 protein stability. SETDB1 is often amplified in primary breast tumours, and its depletion confers to breast cancer cells growth disadvantage. We identified a list of thirty genes repressed by ΔNp63 in a SETDB1-dependent manner, whose expression is positively correlated to survival of breast cancer patients. These results suggest that p63 and SETDB1 expression, together with the repressed genes, may have diagnostic and prognostic potential.
authors: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors: Carla
authors: C
authors: Regina
authors: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors: Mirco
authors: M
authors: Compagnone
authors: CNR, Institute of Cell Biology and Neurobiology (IBCN), Rome, Italy.
authors: Angelo
authors: A
authors: Peschiaroli
authors: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors: AnnaMaria
authors: A
authors: Lena
authors: IDI-IRCCS, Rome, Italy.
authors: Margherita
authors: M
authors: Annicchiarico-Petruzzelli
authors: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors: Maria Cristina
authors: MC
authors: Piro
authors: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.
authors: Gerry
authors: G
authors: Melino
authors: Department of Experimental Medicine and Surgery, University of Rome "Tor Vergata", Rome, Italy.|IDI-IRCCS, Rome, Italy.
authors: Eleonora
authors: E
authors: Candi
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D053208:Kaplan-Meier Estimate; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000235:genetics / Q000378:metabolism*
chemical_list: C081876:CKAP4 protein, human; D008565:Membrane Proteins; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C454062:SETDB1 protein, human
keywords: SETDB1; breast cancer; histone methyl transferase; p63; proliferation