title: Complete genome of a dengue virus serotype 4 strain from Amazonas, Brazil.
abstract: Dengue virus (DENV) infections represent a significant concern for public health worldwide, being considered as the most prevalent arthropod-borne virus regarding the number of reported cases. In this study, we report the complete genome sequencing of a DENV serotype 4 isolate, genotype II, obtained in the city of Manaus, directly from the serum sample, applying Ion Torrent sequencing technology. The use of a massive sequencing technology allowed the detection of two variable sites, one in the coding region for the viral envelope protein and the other in the nonstructural 1 coding region within viral populations.
authors: Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz, Manaus, AM, Brasil.
authors: Valdinete Alves do
authors: VA
authors: Nascimento
authors: Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz, Manaus, AM, Brasil.
authors: Victor Costa de
authors: VC
authors: Souza
authors: Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz, Manaus, AM, Brasil.
authors: Felipe Gomes
authors: FG
authors: Naveca
mesh_terms: D001938:Brazil; D030541:Databases, Genetic; D003715:Dengue / Q000097:blood; D003716:Dengue Virus / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D065288:Serogroup; D012703:Serotyping / Q000379:methods; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins