title: Predicting delayed kidney graft function with gene expression in preimplantation biopsies and first-day posttransplant blood.
abstract: The purpose of this study was to investigate possible markers for predicting delayed graft function (DGF). To this end we analyzed, in pre-implantation biopsies (PIB) and in first-day post-Tx peripheral blood mononuclear cells (PBMC), the expression of five genes (ACSL4, CUBN, DEFB1, FABP3, GK) through real-time TaqMan PCR assays. These genes were selected from a large scale gene expression study in PIB. DEFB1, FABP3 and GK expression levels in PIB were lower in cases with DGF and, in a multivariate analysis which included these genes and clinical variables, only FABP3 expression remained independently associated with DGF. FABP3 expression lower than -1.32 units of relative expression conferred an odds ratio for DGF of 41.1. Compared to the PBMC of recipients without DGF, recipients with prolonged DGF (pDGF) had lower ACSL4 and higher DEFB1 expression levels. In a multivariate analysis, including PBMC gene expression levels of ACSL4, DEFB1 and TLR4 (data from a previous study with the same patients) and clinical variables, only TLR4 remained independently associated with pDGF. In summary, this study revealed FABP3 expression in PIB as a marker for DGF and disclosed new genes possibly involved in the pathogenesis of DGF.
authors: Instituto de Immunogenética - AFIP, São Paulo, SP, Brazil; Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors: Tuíla B
authors: TB
authors: Mourão
authors: Instituto de Immunogenética - AFIP, São Paulo, SP, Brazil.
authors: Karina L
authors: KL
authors: Mine
authors: Instituto de Immunogenética - AFIP, São Paulo, SP, Brazil; Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors: Erika F
authors: EF
authors: Campos
authors: Universidade Federal de São Paulo, São Paulo, SP, Brazil; Hospital do Rim e Hipertensão, São Paulo, SP, Brazil.
authors: Jose O
authors: JO
authors: Medina-Pestana
authors: Hospital do Rim e Hipertensão, São Paulo, SP, Brazil.
authors: Helio
authors: H
authors: Tedesco-Silva
authors: Instituto de Immunogenética - AFIP, São Paulo, SP, Brazil; Universidade Federal de São Paulo, São Paulo, SP, Brazil. Electronic address: gerbase@igen.org.br.
authors: Maria
authors: M
authors: Gerbase-DeLima
mesh_terms: D000328:Adult; D015415:Biomarkers; D001706:Biopsy; D003066:Coenzyme A Ligases / Q000235:genetics; D051799:Delayed Graft Function / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006085:Graft Survival / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D016030:Kidney Transplantation; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D011379:Prognosis; D012372:ROC Curve; D013997:Time Factors; D014019:Tissue Donors; D023083:beta-Defensins / Q000235:genetics; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
chemical_list: D015415:Biomarkers; C406407:DEFB1 protein, human; D023083:beta-Defensins; D003066:Coenzyme A Ligases; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
keywords: Delayed graft function; Gene expression; Kidney transplant; Peripheral blood mononuclear cells; Preimplantation biopsies