title: Allergen-induced Changes in Bone Marrow and Airway Dendritic Cells in Subjects with Asthma.
abstract: Dendritic cells (DCs) are antigen-presenting cells essential for the initiation of T-cell responses. Allergen inhalation increases the number of airway DCs and the release of epithelial-derived cytokines, such as IL-33 and thymic stromal lymphopoietin (TSLP), that activate DCs.
  
  
  
  To examine the effects of inhaled allergen on bone marrow production of DCs and their trafficking into the airways in subjects with allergic asthma, and to examine IL-33 and TSPL receptor expression on DCs.
  
  
  
  Bone marrow, peripheral blood, bronchoalveolar lavage (BAL), and bronchial biopsies were obtained before and after inhalation of diluent and allergen from subjects with asthma that develop allergen-induced dual responses. Classical DCs (cDCs) were cultured from bone marrow CD34(+) cells. cDC1s, cDC2s, and plasmacytoid DCs were measured in bone marrow aspirates, peripheral blood, and BAL by flow cytometry, and cDCs were quantified in bronchial biopsies by immunofluorescence staining.
  
  
  
  Inhaled allergen increased the number of cDCs grown from bone marrow progenitors, and cDCs and plasmacytoid DCs in bone marrow aspirates 24 hours after allergen. Allergen also increased the expression of the TSLP receptor, but not the IL-33 receptor, on bone marrow DCs. Finally, inhaled allergen increased the percentage of cDC1s and cDC2s in BAL but only cDC2s in bronchial tissues.
  
  
  
  Inhaled allergen increases DCs in bone marrow and trafficking of DCs into the airway, which is associated with the development airway inflammation in subjects with allergic asthma. Inhaled allergen challenge also increases expression of TSLP, but not IL-33, receptors on bone marrow DCs.
authors: 1 Firestone Institute of Respiratory Health.|2 Department of Medicine, and.
authors: Amani
authors: A
authors: El-Gammal
authors: 2 Department of Medicine, and.
authors: John-Paul
authors: JP
authors: Oliveria
authors: 2 Department of Medicine, and.
authors: Karen
authors: K
authors: Howie
authors: 2 Department of Medicine, and.
authors: Richard
authors: R
authors: Watson
authors: 2 Department of Medicine, and.
authors: Patrick
authors: P
authors: Mitchell
authors: 1 Firestone Institute of Respiratory Health.|3 Guangzhou Institute of Respiratory Disease, 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors: Ruchong
authors: R
authors: Chen
authors: 2 Department of Medicine, and.
authors: Adrian
authors: A
authors: Baatjes
authors: 2 Department of Medicine, and.
authors: Steven
authors: S
authors: Smith
authors: 4 Department of Pathology and Molecular Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors: Dhuha
authors: D
authors: Al-Sajee
authors: 4 Department of Pathology and Molecular Medicine, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada; and.
authors: Thomas J
authors: TJ
authors: Hawke
authors: 2 Department of Medicine, and.
authors: Kieran J
authors: KJ
authors: Killian
authors: 2 Department of Medicine, and.
authors: Gail M
authors: GM
authors: Gauvreau
authors: 1 Firestone Institute of Respiratory Health.|2 Department of Medicine, and.
authors: Paul M
authors: PM
authors: O'Byrne
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology* / Q000378:metabolism; D001249:Asthma / Q000276:immunology* / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D000067596:Interleukin-33 / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D055815:Young Adult; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D000485:Allergens; D016207:Cytokines; D000067596:Interleukin-33; D000094632:Thymic Stromal Lymphopoietin
keywords: asthma; inhaled allergens; myeloid dendritic cells; plasmacytoid dendritic cells