title: MEK/ERK signaling pathway in apoptosis of SW620 cell line and inhibition effect of resveratrol.
abstract: OBJECTIVE
  To study the involvement of MAPK MEK/ERK signaling transduction pathway in the apoptosis process of SW620 tumor cell line and the inhibition effect of resveratrol.
  
  
  METHODS
  SW620 cell lines were divided into 5 groups, namely, control group, PD98059 group, low-dose resveratrol group, mid-dose resveratrol group and high-dose resveratrol group. The inhibition rate of cell proliferation was detected by MTT method. The expression of apoptotic molecules and MEK/ERK signaling pathway related proteins were assayed by real-time PCR and Western blotting.
  
  
  RESULTS
  Compared with control group, the proliferation of cells treated with resveratrol was significantly inhibited. In the case of apoptotic molecules, the expression of Bax, Caspase 3 and Caspase 9 was increased significantly while the expression of anti-apoptotic molecule Bcl2 was decreased significantly in resveratrol groups with a dose-dependent manner. In the case of molecules in MEK/ERK signaling pathway, the expression of Ras, Raf, MEK and ERK1/2 was decreased significantly in resveratrol groups with a dose-dependent manner.
  
  
  CONCLUSIONS
  PD98059 and resveratrol can effectively inhibit the proliferation of SW620 through inhibiting the MEK/ERK signaling pathway.
authors: Jingzhou Central Hospital, Wuhan City, Hubei Province, China. Electronic address: 643025@qq.com.
authors: Hao
authors: H
authors: Chen
authors: Jingzhou Central Hospital, Wuhan City, Hubei Province, China.
authors: Zhi-Liang
authors: ZL
authors: Jin
authors: Huangjiahu Hospital, Hubei University of Chinese Medicine, Wuhan City, Hubei Province, China.
authors: Hai
authors: H
authors: Xu
keywords: Apoptosis; Colon cancer; Inhibition of proliferation; MEK/ERK signaling pathway; Resveratrol