title: Validation of an automated enzyme immunoassay for the measurement of serum total thyroxine in cats.
abstract: BACKGROUND
  Hyperthyroidism is common in older cats, which necessitates frequent screening of serum total thyroxine (TT4) concentrations. Fast, cheap, and reliable methods to measure TT4 in cats are needed.
  
  
  OBJECTIVE
  The purpose of the study was the validation of a human TT4 enzyme immunoassay (EIA) for use with feline serum, and derivation of a TT4 reference interval (RI) for cats aged 9 years and older.
  
  
  METHODS
  Assay precision, reproducibility, and linearity were evaluated. Interference by hemolysis was also assessed. Method comparison studies between the human EIA and a previously validated radioimmunoassay (RIA) and chemiluminescent-enzyme immunoassay (CEIA) were performed. Healthy cats (> 9 years) were recruited from 3 UK first opinion practices.
  
  
  RESULTS
  The human TT4 EIA demonstrated good precision and reproducibility, and adequate linearity. Hemolysis did not significantly alter measured TT4 concentrations until HGB > 8 g/L. Method comparison revealed proportional and constant errors between EIA and RIA/CEIA. The TT4 RI for cats (> 9 years) was calculated as 7.1-45.1 nmol/L (n = 49).
  
  
  CONCLUSIONS
  The human TT4 EIA was successfully validated for use with feline serum and offers a rapid, cheap, and reliable method for determination of serum TT4 concentrations in cats.
authors: Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
authors: Tim L
authors: TL
authors: Williams
authors: Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
authors: Joy
authors: J
authors: Archer
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis*; D002415:Cats; D005260:Female; D006980:Hyperthyroidism / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D008297:Male; D011863:Radioimmunoassay / Q000662:veterinary; D012016:Reference Values; D015203:Reproducibility of Results; D013960:Thyroid Function Tests / Q000662:veterinary; D013974:Thyroxine / Q000097:blood*
chemical_list: D013974:Thyroxine
keywords: Analyzer; feline; human; hyperthyroidism; reference interval