title: MicroRNA-187-3p mitigates non-small cell lung cancer (NSCLC) development through down-regulation of BCL6.
abstract: Hsa-microRNA-187-3p (miR-187-3p) has recently been discovered having anticancer efficacy in different organs. However, the role of miR-187-3p on non-small cell lung cancer (NSCLC) is still ambiguous. In this study, we investigated the role of miR-187-3p on the development of NSCLC. The results indicated that miR-187-3p was significantly down-regulated in primary tumor tissues and very low levels were found in NSCLC cell lines. Ectopic expression of miR-187-3p in NSCLC cell lines significantly suppressed cell growth as evidenced by cell viability assay and colony formation assay, through inhibition of BCL6. In addition, miR-187-3p induced apoptosis, as indicated by concomitantly with up-regulation of the activities of caspase-3 and caspase-7, and inhibited cellular migration and invasiveness through inhibition of BCL6. Further, oncogene BCL6 was revealed to be a putative target of miR-187-3p, which was inversely correlated with miR-187-3p expression in NSCLC. Taken together, our results demonstrated that miR-187-3p played a pivotal role on NSCLC through inhibiting cell proliferation, migration, invasion, and promoting apoptosis by targeting oncogenic BCL6.
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, PR China.
authors: Chengcao
authors: C
authors: Sun
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, PR China; Wuhan Hospital for the Prevention and Treatment of Occupational Diseases, 430071 Wuhan, PR China.
authors: Shujun
authors: S
authors: Li
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, PR China.
authors: Cuili
authors: C
authors: Yang
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, PR China.
authors: Yongyong
authors: Y
authors: Xi
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, PR China.
authors: Liang
authors: L
authors: Wang
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, PR China.
authors: Feng
authors: F
authors: Zhang
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, PR China. Electronic address: lodjlwhu@sina.com.
authors: Dejia
authors: D
authors: Li
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D051560:Proto-Oncogene Proteins c-bcl-6
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; C571843:MIRN187 microRNA, human; D035683:MicroRNAs; D051560:Proto-Oncogene Proteins c-bcl-6
keywords: Apoptosis; BCL6; Hsa-miRNA-187-3p (miR-187-3p); Non-small cell lung cancer (NSCLC); Proliferation