title: Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers.
abstract: We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m(2)). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m(2)). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.
authors: Biobased Colloids and Materials (BiCMat), Department of Forest Products Technology, School of Chemical Technology, Aalto University , FI-00076, Espoo, Finland.
authors: Maija
authors: M
authors: Vuoriluoto
authors: Biobased Colloids and Materials (BiCMat), Department of Forest Products Technology, School of Chemical Technology, Aalto University , FI-00076, Espoo, Finland.
authors: Hannes
authors: H
authors: Orelma
authors: DWI-Leibniz-Institute for Interactive Materials Research , Forckenbeckstr. 50, D-52056 Aachen, Germany.
authors: Baolei
authors: B
authors: Zhu
authors: Biobased Colloids and Materials (BiCMat), Department of Forest Products Technology, School of Chemical Technology, Aalto University , FI-00076, Espoo, Finland.
authors: Leena-Sisko
authors: LS
authors: Johansson
authors: Biobased Colloids and Materials (BiCMat), Department of Forest Products Technology, School of Chemical Technology, Aalto University , FI-00076, Espoo, Finland.
authors: Orlando J
authors: OJ
authors: Rojas
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D008689:Methacrylates / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000276:immunology; D013211:Staphylococcus aureus / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D007074:Immunoglobulin G; D008689:Methacrylates; D009757:Nylons; D013205:Staphylococcal Protein A; C407037:poly(2-(dimethylamino)ethyl methacrylate); D014867:Water; D002482:Cellulose
keywords: PDMAEMA; POEGMA; TEMPO-oxidation; antifouling; biosurfaces; cellulose nanofibrils; human IgG; nonspecific adsorption