title: Inhibitory gene expression of the Cav3.1 T-type calcium channel to improve neuronal injury induced by lidocaine hydrochloride.
abstract: Cav3.1 is a low-voltage-activated (LVA) calcium channel that plays a key role in regulating intracellular calcium ion levels. In this study, we observed the effects of lidocaine hydrochloride on the pshRNA-CACNA1G-SH-SY5Y cells that silenced Cav3.1 mRNA by RNA interference, and investigated the roles of p38 MAPK in these effects. We constructed the pNC-puro-CACNA1G-SH-SY5Y cells and pshRNA-CACNA1G -SH-SY5Y cells by the RNA interference. All the cells were cultured with or without 10mM lidocaine hydrochloride for 24 h. The cell morphology, cell viability, Cav3.1 and p38 protein expression, cell apoptosis rate and intracellular calcium ion concentration were detected. We found that all cells treated with 10mM lidocaine hydrochloride for 24 h showed cellular rounding, axonal regression, and cellular floating. Compared with the cells in SH-SY5Y+Lido group and NC+Lido group, those in the RNAi+Lido group showed similar changes, but of smaller magnitude. Additionally, following lidocaine hydrochloride all cells displayed increased Cav3.1 and p38 MAPK protein, apoptosis rate, and intracellular calcium ion levels; however,these changes in the RNAi+Lido group were less pronounced than in the SH-SY5Y+Lido and NC+Lido groups. The cell viability decreased following lidocaine hydrochloride treatment, but viability of the cells in the RNAi+Lido group was higher than in the SH-SY5Y+Lido and NC+Lido groups. The results showed that Cav3.1 may be involved in neuronal injury induced by lidocaine hydrochloride and that p38 MAPK phosphorylation was reduced upon Cav3.1 gene silencing.
authors: Department of Anesthesiology, First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors: Xianjie
authors: X
authors: Wen
authors: Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China. Electronic address: wxjie@fsyyy.com.
authors: Shiyuan
authors: S
authors: Xu
authors: Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors: Qingguo
authors: Q
authors: Zhang
authors: Department of Pain Clinic, First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors: Xiaohong
authors: X
authors: Li
authors: Department of Anesthesiology, First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors: Hua
authors: H
authors: Liang
authors: Department of Anesthesiology, First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors: Chenxiang
authors: C
authors: Yang
authors: Department of Anesthesiology, First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors: Hanbing
authors: H
authors: Wang
authors: Department of Anesthesiology, First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University, Foshan 528000, Guangdong Province, China.
authors: Hongzhen
authors: H
authors: Liu
mesh_terms: D000779:Anesthetics, Local / Q000009:adverse effects* / Q000494:pharmacology; D017209:Apoptosis; D020747:Calcium Channels, T-Type / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008012:Lidocaine / Q000009:adverse effects* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D010766:Phosphorylation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000779:Anesthetics, Local; C121855:CACNA1G protein, human; D020747:Calcium Channels, T-Type; D008012:Lidocaine; D048051:p38 Mitogen-Activated Protein Kinases
keywords: 2-(4-Amidinophenyl)−6-indolecarbamidine dihydrochloride (PubChem CID: 160166; Calcium ion (PubChem CID: 271); Cav3.1; Lidocaine hydrochloride; Lidocaine hydrochloride (PubChem CID: 6314); Propidium iodide (PubChem CID: 104981); SH-SY5Y cells; p38 MAPK