title: CXCL12/CXCR4 display an inverse mRNA expression profile in gastric carcinoma that correlates with tumor progression.
abstract: Chemokines and their receptors have been shown to contribute to tumor growth and metastatic spread in various gastrointestinal cancer entities. In the present study, the mRNA expression profiles and clinical significance of chemokine ligand CXCL12 and its corresponding receptor CXCR4 were investigated in patients with gastric cancer (GC). Using quantitative polymerase chain reaction, the expression profile of CXCL12/CXCR4 was analyzed in resection specimens from the patients with GC (n=66) and in corresponding normal gastric tissues. Upon investigating CXCL12/CXCR4 mRNA expression levels in the GC tissues, significant downregulation of CXCL12 expression was demonstrated (P<0.05), whereas CXCR4 mRNA expression was shown to be significantly upregulated (P<0.05). Likewise, in gastric carcinoma patients undergoing neoadjuvant chemotherapy, CXCR4 expression was found to be significantly upregulated (P<0.05), whereas in GC patients with lymph and vein infiltration, CXCL12 mRNA expression was significantly downregulated (P<0.05). These results demonstrate a significant inverse association between the development and progress of GC and CXCL12/CXCR4 mRNA expression. CXCR4 mRNA upregulation was promoted under the effect of neoadjuvant chemotherapy prior to surgery in GC patients, whereas higher tumor stages with lymph and vein infiltration negatively affected CXCL12 mRNA expression.
authors: Department of General, Visceral, Vascular and Pediatric Surgery, University of The Saarland, Homburg/Saar D-66421, Germany.
authors: Claudia
authors: C
authors: Rubie
authors: Department of General, Visceral, Vascular and Pediatric Surgery, University of The Saarland, Homburg/Saar D-66421, Germany.
authors: Anne
authors: A
authors: Kauffels
authors: Department of General, Visceral, Vascular and Pediatric Surgery, University of The Saarland, Homburg/Saar D-66421, Germany.
authors: Kathrin
authors: K
authors: Kölsch
authors: Department of General, Visceral, Vascular and Pediatric Surgery, University of The Saarland, Homburg/Saar D-66421, Germany.
authors: Mathias
authors: M
authors: Glanemann
authors: Department of General, Visceral, Vascular and Pediatric Surgery, University of The Saarland, Homburg/Saar D-66421, Germany.
authors: Christoph
authors: C
authors: Justinger
keywords: CXCL12; CXCR4; gastric carcinoma; gene expression