title: The generation of human innate lymphoid cells is influenced by the source of hematopoietic stem cells and by the use of G-CSF.
abstract: NK cells play a central role in the haploidentical HSC transplantation (HSCT) to cure high-risk leukemias. Other innate lymphoid cells (ILCs) have been proposed to exert a protective role in graft-versus-host disease and could also contribute to anti-microbial defence and to lymphoid tissue remodeling. Thus, we investigated the ILC differentiation potential of HSCs isolated from BM, mobilized peripheral blood (PB), and umbilical cord blood (UCB). BM CD34(+) cells are enriched in lymphoid-committed precursors, while PB CD34(+) cells preferentially contain myeloid precursors. In vitro differentiation experiments revealed that the highest and the lowest CD56(+) CD161(+) ILC recovery was detected in UCB and PB HSC cultures, respectively. Among CD56(+) CD161(+) ILCs, the ratio between NK cells and ILC3s was similar for all HSC analyzed. ILC recovery in PB CD34(+) cultures was lower for G-CSF-mobilized HSCs (good mobilizers) than for G-CSF+plerixafor-mobilized HSC (poor mobilizers). Moreover, G-CSF inhibited in vitro ILC recovery and the degree of inhibition was proportional to the time of exposure to the cytokine. Thus, although all common sources of HSC for transplant differentiate towards ILCs, substantial differences exist among different sources and G-CSF may influence ILC recovery. These data offer new clues for a better understanding of the immune reconstitution after HSCT.
authors: Department of Internal Medicine, University of Verona, Verona, Italy.|Lab. Analisi chimico-cliniche e microbiologiche, Ospedale Sacro Cuore Negrar, Verona, Italy.
authors: Francesca
authors: F
authors: Moretta
authors: U.O.C. Clinical and Experimental Immunology IPRCCS, Giannina Gaslini Institute, Genova, Italy.
authors: Francesca
authors: F
authors: Petronelli
authors: Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors: Barbarella
authors: B
authors: Lucarelli
authors: Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors: Angela
authors: A
authors: Pitisci
authors: Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors: Alice
authors: A
authors: Bertaina
authors: Department of Pediatric Hematology and Oncology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.|Department of Pediatrics, University of Pavia, Pavia, Italy.
authors: Franco
authors: F
authors: Locatelli
authors: U.O. Immunology, IRCCS AOU San Martino-IST, Genova, Italy.|Department of Experimental Medicine, University of Genova, Genova, Italy.
authors: Maria Cristina
authors: MC
authors: Mingari
authors: Department of Immunology, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
authors: Lorenzo
authors: L
authors: Moretta
authors: U.O.C. Clinical and Experimental Immunology IPRCCS, Giannina Gaslini Institute, Genova, Italy.
authors: Elisa
authors: E
authors: Montaldo
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology; D001596:Benzylamines; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D019002:CD56 Antigen / Q000276:immunology; D002452:Cell Count; D000080027:Cyclams; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology; D006086:Graft vs Host Disease; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology*; D006571:Heterocyclic Compounds / Q000494:pharmacology; D006801:Humans; D007113:Immunity, Innate; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology*; D038041:Lymphopoiesis; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000276:immunology; D010641:Phenotype
chemical_list: D018952:Antigens, CD34; D001596:Benzylamines; D019002:CD56 Antigen; D000080027:Cyclams; D006571:Heterocyclic Compounds; D055651:NK Cell Lectin-Like Receptor Subfamily B; D016179:Granulocyte Colony-Stimulating Factor; C088327:plerixafor
keywords: G-CSF · HSCT · ILC · ILC development · NK cells · Plerixafor