title: Expression of type 2 diacylglycerol acyltransferse gene DGTT1 from Chlamydomonas reinhardtii enhances lipid production in Scenedesmus obliquus.
abstract: Microalgal strains of Scenedesmus obliquus have the great potential for the production of biofuels, CO2 fixation, and bioremediation. However, metabolic engineering of S. obliquus to improve their useful phenotypes are still not fully developed. In this study, S. obliquus strain CPC2 was genetically engineered to promote the autotrophic growth and lipid productivity. The overexpression plasmid containing the type 2 diacylglycerol acyltransferse (DGAT) gene DGTT1 from Chlamydomonas reinhardtii was constructed and transformed into S. obliquus CPC2, and the positive transformants were obtained. The expression of DGTT1 gene was confirmed by reverse transcription PCR analysis. Enhanced lipid content of the transformant S. obliquus CPC2-G1 by nearly two-fold was observed. The biomass concentration of the recombinant strains was also 29% higher than that of the wild-type strain. Furthermore, the recombinant strain CPC2-G1 was successfully grown in 40 L tubular type photobioreactor and open pond system in an outdoor environment. The lipid content, biomass concentration, and biomass productivity obtained from 40 L tubular PBR were 127.8% 20.0%, and 232.6% higher than those obtained from the wild-type strain. The major aim of this work is to develop a tool to genetically engineer an isolated S. obliquus strain for the desired purpose. This is the first report that genetic engineering of S. obliquus has been successful employed to improve both the microalgal cell growth and the lipid production.
authors: University Center of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors: Chun-Yen
authors: CY
authors: Chen
authors: Department of Biotechnology, Green Technology Research Institute, CPC Corporation, Taiwan.
authors: Ai-Ling
authors: AL
authors: Kao
authors: Department of Biotechnology, Green Technology Research Institute, CPC Corporation, Taiwan.
authors: Zheng-Chia
authors: ZC
authors: Tsai
authors: Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan.
authors: Te-Jin
authors: TJ
authors: Chow
authors: University Center of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors: Hsin-Yueh
authors: HY
authors: Chang
authors: School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
authors: Xin-Qing
authors: XQ
authors: Zhao
authors: Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan.
authors: Po-Ting
authors: PT
authors: Chen
authors: Department of Biotechnology, Fooyin University, Kaohsiung, Taiwan.|Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, Taiwan.
authors: Hsiang-Yen
authors: HY
authors: Su
authors: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan. changjs@mail.ncku.edu.tw.|Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, Taiwan. changjs@mail.ncku.edu.tw.
authors: Jo-Shu
authors: JS
authors: Chang
mesh_terms: D018533:Biomass; D016825:Chlamydomonas reinhardtii / Q000201:enzymology* / Q000235:genetics; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D044444:Scenedesmus / Q000235:genetics / Q000254:growth & development*; D014170:Transformation, Genetic
chemical_list: D008055:Lipids; D010940:Plant Proteins; D051048:Diacylglycerol O-Acyltransferase
keywords: Chlamydomonas reinhardtii; Diacylglycerol acyltransferse gene DGTT1; Genetic engineering; Microalgal lipids; Scenedesmus obliquus