title: Association of Increased Treg Cell Levels With Elevated Indoleamine 2,3-Dioxygenase Activity and an Imbalanced Kynurenine Pathway in Interferon-Positive Primary Sjögren's Syndrome.
abstract: Indoleamine 2,3-dioxygenase (IDO), the rate-limiting enzyme that converts tryptophan to kynurenine, is driven in part by type I and type II interferons (IFNs). Naive T cells are polarized into FoxP3+ Treg cells upon exposure to either IDO+ cells or kynurenine. Recent studies have suggested that the kynurenine pathway reflects a crucial interface between the immune and nervous system. The aims of the present study were to evaluate whether Treg cell levels are elevated, in conjunction with increased IDO activity, in patients with primary Sjögren's syndrome (SS) who are positive for the IFN gene expression signature, and to investigate the downstream kynurenine pathway in these patients.
  
  
  
  Serum from 71 healthy controls, 58 IFN-negative patients with primary SS, and 66 IFN-positive patients with primary SS was analyzed using high-performance liquid chromatography to measure the levels of tryptophan and kynurenine. Expression levels of messenger RNA (mRNA) for IDO and downstream enzymes in the kynurenine pathway were assessed in CD14+ monocytes using real-time quantitative polymerase chain reaction. CD4+CD45RO+ T helper memory cell populations were analyzed by flow cytometry.
  
  
  
  Significantly increased levels of IDO activity (assessed as the kynurenine:tryptophan ratio) (P = 0.0054) and percentages of CD25(high) FoxP3+ Treg cells (P = 0.039) were observed in the serum from IFN-positive patients with primary SS, and these parameters were significantly correlated with one another (r = 0.511, P = 0.002). In circulating monocytes from IFN-positive patients with primary SS, the expression of IDO1 mRNA was up-regulated (P < 0.0001), and this was correlated with the IFN gene expression score (r = 0.816, P < 0.0001). Interestingly, the proapoptotic and neurotoxic downstream enzyme kynurenine 3-monooxygenase was up-regulated (P = 0.0057), whereas kynurenine aminotransferase I (KATI) (P = 0.0003), KATIII (P = 0.016), and KATIV (P = 0.04) were down-regulated in IFN-positive patients with primary SS compared to healthy controls.
  
  
  
  These findings demonstrate enhanced IDO activity in conjunction with increased percentages of CD25(high) FoxP3+ Treg cells in primary SS patients who carry the IFN signature. In addition, IFN-positive patients with primary SS exhibit an imbalanced kynurenine pathway, with evidence of a shift toward potentially more proapoptotic and neurotoxic metabolites. Intervening in these IFN- and IDO-induced immune system imbalances may offer a new array of possibilities for therapeutic interventions in patients with primary SS.
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Naomi I
authors: NI
authors: Maria
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Cornelia G
authors: CG
authors: van Helden-Meeuwsen
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Zana
authors: Z
authors: Brkic
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Sandra M J
authors: SM
authors: Paulissen
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Eline C
authors: EC
authors: Steenwijk
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Virgil A
authors: VA
authors: Dalm
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Paul L
authors: PL
authors: van Daele
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: P
authors: P
authors: Martin van Hagen
authors: University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
authors: Frans G M
authors: FG
authors: Kroese
authors: University Medical Center Utrecht, Utrecht, The Netherlands.
authors: Joel A G
authors: JA
authors: van Roon
authors: Trinity College Dublin, Institute of Neuroscience, Dublin, Ireland.
authors: Andrew
authors: A
authors: Harkin
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Willem A
authors: WA
authors: Dik
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Hemmo A
authors: HA
authors: Drexhage
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Erik
authors: E
authors: Lubberts
authors: Erasmus Medical Center, Rotterdam, The Netherlands.
authors: Marjan A
authors: MA
authors: Versnel
mesh_terms: D005260:Female; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism*; D007372:Interferons / Q000097:blood*; D007737:Kynurenine / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction; D012859:Sjogren's Syndrome / Q000097:blood* / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000201:enzymology*
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007737:Kynurenine; D007372:Interferons