title: Induced Pluripotent Stem Cell Labeling Using Quantum Dots.
abstract: Induced pluripotent stem (iPS) cells have received remarkable attention as the cell sources for clinical applications of regenerative medicine including stem cell therapy. Additionally, labeling technology is in high demand for tracing transplanted cells used in stem cell therapy. In this study, we used quantum dots (QDs), which have distinct fluorescence abilities in comparison with traditional probes, as the labeling materials and investigated whether iPS cells could be labeled with QDs with no cytotoxicity. iPS cells could not be labeled with QDs alone but required the use of cell-penetrating peptides such as octaarginine (R8). No significant cytotoxicity to iPS cells was confirmed by up to 8 nM QDs, and the iPS cells labeled with QDs maintained their undifferentiated state and pluripotency. These data suggest that QDs can be used for fluorescence labeling of iPS cells.
authors: FIRST Research Center for Innovative Nanobiodevices, Nagoya University , Furo-cho, Chikusa-ku, Nagoya , Japan.
authors: Hiroshi
authors: H
authors: Yukawa
authors: FIRST Research Center for Innovative Nanobiodevices, Nagoya University , Furo-cho, Chikusa-ku, Nagoya , Japan.
authors: Kaoru
authors: K
authors: Suzuki
authors: † Department of Medical Technology, Nagoya University, Graduate School of Medicine , Daikominami, Higashi-ku, Nagoya , Japan.
authors: Yuki
authors: Y
authors: Kano
authors: † Department of Medical Technology, Nagoya University, Graduate School of Medicine , Daikominami, Higashi-ku, Nagoya , Japan.
authors: Tatsuya
authors: T
authors: Yamada
authors: FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan; ‡Department of Applied Chemistry, Nagoya University, Graduate School of Engineering, Furo-cho, Chikusa-ku, Nagoya, Japan.
authors: Noritada
authors: N
authors: Kaji
authors: † Department of Medical Technology, Nagoya University, Graduate School of Medicine , Daikominami, Higashi-ku, Nagoya , Japan.
authors: Tetsuya
authors: T
authors: Ishikawa
authors: FIRST Research Center for Innovative Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan; ‡Department of Applied Chemistry, Nagoya University, Graduate School of Engineering, Furo-cho, Chikusa-ku, Nagoya, Japan; §Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho, Takamatsu, Japan.
authors: Yoshinobu
authors: Y
authors: Baba
keywords: Induced pluripotent stem (iPS) cells; Octaarginine (R8); Quantum dots (QDs)