title: Prolonged Mouse Cardiac Graft Cold Storage via Attenuating Ischemia-Reperfusion Injury Using a New Antioxidant-Based Preservation Solution.
abstract: One of the major events in ischemia-reperfusion (I/R)-induced heart injury in cardiac transplantation is the generation of reactive oxygen species. We hypothesized that a novel preservation solution called SBI-SEIIKU II (SS-II) contains 3 antioxidant reagents: L-cysteine, glycine, ascorbic acid/ascorbic acid-2-phosphate magnesium, which can block the generation of reactive oxygen species to result in a prolongation of the cold storage time via attenuating I/R injury.
  
  
  
  C57BL/6CrSlc(B6) mice underwent syngeneic mice heterotopic heart transplantation, and the animals were derived into 3 groups: recipients with nonpreserved grafts (control group), recipients with grafts preserved in histidine-tryptophan-ketoglutarate (HTK) for 24 and 48 hours (HTK group), and recipients with grafts preserved in SS-II for 24 and 48 hours (SS-II group).
  
  
  
  After 48 hours of preservation, there were no grafts that survived in the HTK group; however, the SS-II group had a high survival rate. After 24 hours of preservation, SS-II decreased the oxidative damage, myocardial apoptosis, and the infiltration of macrophages and neutrophils in the cardiac grafts in the early phase and suppressed the development of myocardial fibrosis in long-term grafts compared with HTK.
  
  
  
  The SS-II prolongs the acceptable cold storage time and protects the myocardium from I/R injury via inhibiting oxidative stress-associated damage. We believe that this novel preservation solution may be simple and safe for use in the clinical transplantation field.
authors: 1 Division of Transplantation Immunology, National Research Institute for Child Health and Development, Tokyo, Japan. 2 Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Osaka, Japan. 3 AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan. 4 SBI Pharmaceuticals Co., Ltd., Tokyo, Japan. 5 Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan.
authors: Songjie
authors: S
authors: Cai
authors: Naotsugu
authors: N
authors: Ichimaru
authors: Mingyi
authors: M
authors: Zhao
authors: Masayuki
authors: M
authors: Fujino
authors: Hidenori
authors: H
authors: Ito
authors: Urara
authors: U
authors: Ota
authors: Motowo
authors: M
authors: Nakajima
authors: Tohru
authors: T
authors: Tanaka
authors: Norio
authors: N
authors: Nonomura
authors: Xiao-Kang
authors: XK
authors: Li
authors: Shiro
authors: S
authors: Takahara
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D017209:Apoptosis; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D003080:Cold Temperature; D015925:Cryopreservation; D003545:Cysteine / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D006085:Graft Survival; D006321:Heart / Q000187:drug effects; D006335:Heart Injuries / Q000473:pathology; D016027:Heart Transplantation / Q000379:methods*; D051547:Heme Oxygenase-1 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000628:therapy*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D008565:Membrane Proteins; D019188:Organ Preservation Solutions; D009943:Organophosphorus Compounds; D017382:Reactive Oxygen Species; C082020:ascorbate-2-polyphosphate; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D008274:Magnesium; D003545:Cysteine; D001205:Ascorbic Acid; D005998:Glycine