title: Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region.
abstract: The T helper type 2 (Th2) locus control region (LCR) regulates Th2 cell differentiation. Several transcription factors bind to the LCR to modulate the expression of Th2 cytokine genes, but the molecular mechanisms behind Th2 cytokine gene regulation are incompletely understood. Here, we used database analysis and an oligonucleotide competition/electrophoretic mobility shift assays to search for transcription factors binding to RHS5, a DNase I hypersensitive site (DHS) within the Th2 LCR. Consequently, we demonstrated that GATA-binding protein-3 (GATA-3), E26 transformation-specific protein 1 (Ets-1), octamer transcription factor-1 (Oct-1), and Oct-2 selectively associate with RHS5. Furthermore, chromatin immunoprecipitation and luciferase reporter assays showed that Oct-1 and Oct-2 bound within the Il4 promoter region and the Th2 LCR, and that Oct-1 and GATA-3 or Oct-2 synergistically triggered the transactivational activity of the Il4 promoter through RHS5. These results suggest that Oct-1 and GATA-3/Oct-2 direct Th2 cytokine gene expression in a cooperative manner.
authors: Department of Life Science, Sogang University, Seoul, Korea.
authors: Kiwan
authors: K
authors: Kim
authors: Department of Life Science, Sogang University, Seoul, Korea.
authors: Najung
authors: N
authors: Kim
authors: Department of Life Science, Sogang University, Seoul, Korea.
authors: Gap Ryol
authors: GR
authors: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D019901:Locus Control Region / Q000502:physiology*; D051379:Mice; D050811:Octamer Transcription Factor-1 / Q000235:genetics / Q000378:metabolism*; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D000075102:Organic Cation Transporter 2; D018418:Th2 Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D050811:Octamer Transcription Factor-1; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; C494166:Pou2f1 protein, mouse; C491012:Slc22a2 protein, mouse; D015847:Interleukin-4