title: Long noncoding RNA OR3A4 promotes metastasis and tumorigenicity in gastric cancer.
abstract: The contribution of long noncoding RNAs (lncRNAs) to metastasis of gastric cancer remains largely unknown. We used microarray analysis to identify lncRNAs differentially expressed between normal gastric tissues and gastric cancer tissues and validated these differences in quantitative real-time (qRT)-PCR experiments. The expression levels of lncRNA olfactory receptor, family 3, subfamily A, member 4 (OR3A4) were significantly associated with lymphatic metastasis, the depth of cancer invasion, and distal metastasis in 130 paired gastric cancer tissues. The effects of OR3A4 were assessed by overexpressing and silencing OR3A4 in gastric cancer cells. OR3A4 promoted cancer cell growth, angiogenesis, metastasis, and tumorigenesis in vitro and in vivo. Global microarray analysis combined with RT-PCR, RNA immunoprecipitation, and RNA pull-down analyses after OR3A4 transfection demonstrated that OR3A4 influenced biologic functions in gastric cancer cells via regulating the activation of PDLIM2, MACC1, NTN4, and GNB2L1. Our results reveal OR3A4 as an oncogenic lncRNA that promotes tumor progression, Therefore, lncRNAs might function as key regulatory hubs in gastric cancer progression.
authors: Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors: Xiaobo
authors: X
authors: Guo
authors: Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors: Ziguo
authors: Z
authors: Yang
authors: Departments of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Qiaoming
authors: Q
authors: Zhi
authors: Departments of Science and Education, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors: Dan
authors: D
authors: Wang
authors: Department of Hepatobiliary Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China.
authors: Lei
authors: L
authors: Guo
authors: Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors: Guimei
authors: G
authors: Li
authors: Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors: Ruizhen
authors: R
authors: Miao
authors: Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors: Yulong
authors: Y
authors: Shi
authors: Departments of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors: Yuting
authors: Y
authors: Kuang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D063646:Carcinogenesis / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008840:Microfilament Proteins / Q000235:genetics; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics; D000075385:Netrins / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D000075370:Receptors for Activated C Kinase / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics; D014183:Transplantation, Heterologous; D047368:Tumor Burden / Q000235:genetics
chemical_list: D060588:LIM Domain Proteins; C544162:MACC1 protein, human; D008840:Microfilament Proteins; C498815:NTN4 protein, human; D009363:Neoplasm Proteins; D000075385:Netrins; C502929:PDLIM2 protein, human; C419368:RACK1 protein, human; D062085:RNA, Long Noncoding; D000075370:Receptors for Activated C Kinase; D015534:Trans-Activators; D014157:Transcription Factors
keywords: OR3A4; gastric cancer; lncRNA; metastasis; tumorigenesis