title: Biotic and abiotic dynamics of a high solid-state anaerobic digestion box-type container system.
abstract: A solid-state anaerobic digestion box-type container system for biomethane production was observed in 12 three-week batch fermentations. Reactor performance was monitored using physico-chemical analysis and the methanogenic community was identified using ANAEROCHIP-microarrays and quantitative PCR. A resilient community was found in all batches, despite variations in inoculum to substrate ratio, feedstock quality, and fluctuating reactor conditions. The consortia were dominated by mixotrophic Methanosarcina that were accompanied by hydrogenotrophic Methanobacterium, Methanoculleus, and Methanocorpusculum. The relationship between biotic and abiotic variables was investigated using bivariate correlation analysis and univariate analysis of variance. High amounts of biogas were produced in batches with high copy numbers of Methanosarcina. High copy numbers of Methanocorpusculum and extensive percolation, however, were found to negatively correlate with biogas production. Supporting these findings, a negative correlation was detected between Methanocorpusculum and Methanosarcina. Based on these results, this study suggests Methanosarcina as an indicator for well-functioning reactor performance.
authors: Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria; Hochschule Hamm-Lippstadt, Biotechnologie, Marker Allee 76-78, 59063 Hamm, Germany. Electronic address: a.walter@uibk.ac.at.
authors: Andreas
authors: A
authors: Walter
authors: Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria. Electronic address: Maraike.Probst@uibk.ac.at.
authors: Maraike
authors: M
authors: Probst
authors: Müller Abfallprojekte GmbH, Hauptstraße 34, 4675 Weibern, Austria. Electronic address: stephan.hinterberger@mueller-umwelttechnik.at.
authors: Stephan
authors: S
authors: Hinterberger
authors: Müller Abfallprojekte GmbH, Hauptstraße 34, 4675 Weibern, Austria. Electronic address: mueller.sen@mueller-umwelttechnik.at.
authors: Horst
authors: H
authors: Müller
authors: Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria. Electronic address: Heribert.Insam@uibk.ac.at.
authors: Heribert
authors: H
authors: Insam
mesh_terms: D000693:Anaerobiosis; D001317:Austria; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D004867:Equipment Design; D005285:Fermentation; D008697:Methane / Q000096:biosynthesis*; D017020:Methanosarcina / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012037:Refuse Disposal / Q000295:instrumentation / Q000379:methods*
chemical_list: D056804:Biofuels; D008697:Methane
keywords: Anaerobic digestion; High solid-state; Methanogens; Microarray; Percolation; qPCR