title: Characterisation of the inflammatory response in Dupuytren's disease.
abstract: BACKGROUND
  Dupuytren's disease is characterised by fibrotic nodule and cord formation in the palmar aponeurosis. The pathophysiology of the disease is still unknown, although cell stress and subsequent activation of immune mechanisms seems to be crucial.
  
  
  METHODS
  Surgically obtained tissue and blood samples of 100 Dupuytren patients were processed by immunohistochemistry, flow cytometry, as well as immunoscope analysis. Macroscopically normal aponeurotic tissue served as control.
  
  
  RESULTS
  Locally, microvascular alterations and massive infiltration by mononuclear cells (CD3+, CD4 > CD8, CD45RO > CD45RA, S100 protein, CD56, CD68, scarce CD19 and mast cells) forming perivascular clusters were found in DD tissue. Cytokine profiling of fibromatosis tissue-derived T-cells showed a Th1/TH17-weighted immune response. Immunoscope analysis revealed a restricted T-cell receptor α/β repertoire pointing to an (auto)antigen-driven process.
  
  
  CONCLUSIONS
  The striking accumulation of immune cells, expression of leukocyte adhesion molecules, as well as pro-inflammatory and pro-fibrotic cytokines near markedly narrowed vessels supports the theory that the abnormal proliferation of fibroblasts and production of extracellular matrix proteins in DD seems to be related to immune-mediated microvascular damage. The restricted T-cell receptor repertoire of intra-lesional T-cells points to an antigen-driven process. T-cells seem to play an important role in the development of Dupuytren's disease.
authors: a Laboratory of Autoimmunity, Division of Experimental Pathophysiology and Immunology, Biocenter , Medical University of Innsbruck , Innsbruck , Austria .|b Department of Internal Medicine III - Cardiology and Angiology , Medical University of Innsbruck , Innsbruck , Austria .
authors: Christina
authors: C
authors: Mayerl
authors: c Department of Plastic, Reconstructive and Aesthetic Surgery , Medical University of Innsbruck , Innsbruck , Austria , and.
authors: Barbara
authors: B
authors: Del Frari
authors: d Institute of Legal Medicine , Medical University of Innsbruck , Innsbruck , Austria.
authors: Walther
authors: W
authors: Parson
authors: a Laboratory of Autoimmunity, Division of Experimental Pathophysiology and Immunology, Biocenter , Medical University of Innsbruck , Innsbruck , Austria .
authors: Guenther
authors: G
authors: Boeck
authors: c Department of Plastic, Reconstructive and Aesthetic Surgery , Medical University of Innsbruck , Innsbruck , Austria , and.
authors: Hildegunde
authors: H
authors: Piza-Katzer
authors: a Laboratory of Autoimmunity, Division of Experimental Pathophysiology and Immunology, Biocenter , Medical University of Innsbruck , Innsbruck , Austria .
authors: Georg
authors: G
authors: Wick
authors: c Department of Plastic, Reconstructive and Aesthetic Surgery , Medical University of Innsbruck , Innsbruck , Austria , and.
authors: Dolores
authors: D
authors: Wolfram
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D018834:Chaperonin 60 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004387:Dupuytren Contracture / Q000276:immunology* / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D008875:Middle Aged; D018417:Th1 Cells / Q000378:metabolism; D058504:Th17 Cells / Q000378:metabolism; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; D018834:Chaperonin 60; D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D006684:HLA-DR Antigens; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing)
keywords: Palmar fibromatosis; immune cells; inflammation