title: Extraction of Angelica sinensis polysaccharides using ultrasound-assisted way and its bioactivity.
abstract: In the present article, ultrasound-assisted extraction (UAE) of polysaccharides from Angelica sinensis were investigated. The aim of the study is to examine the extraction parameters such as ultrasound power (140-180W), the ratio of liquid to solid (5-7), extraction time (40-50min) and extraction temperature (80-100°C) and to obtain the best possible combinations of these parameters through response surface methodology (RSM). Based on contour plots and variance analysis, optimum operational conditions for maximizing polysaccharides yield were found to be 180w, 7, 45min and 90°C. Under the optimum operating conditions determined, 6.96% polysaccharides were achieved. In addition, the results showed that A. sinensis polysaccharides (ASP) could increase antioxidant enzymes activities and decrease the MDA levels in the skeletal muscle of exhaustive exercise rats. This study provides strong evidence that A. sinensis polysaccharides supplementation possessed protective effects against exhaustive exercise-induced oxidative stress.
authors: Shanghai Jiaotong University, Shanghai 200030, China.
authors: Ying
authors: Y
authors: Zhao
authors: Shanghai Jiaotong University, Shanghai 200030, China.
authors: Yongyong
authors: Y
authors: Shi
authors: Harbin Sport University, Shanghai 200030, China; Heilongjiang University Of Chinese Medicine, Shanghai 200030, China.
authors: Huixin
authors: H
authors: Yang
authors: Shanghai University of Sport, Shanghai 200030, China. Electronic address: yzhaogut@sina.com.
authors: Lijuan
authors: L
authors: Mao
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D004365:Drugs, Chinese Herbal; D005163:Factor Analysis, Statistical; D005978:Glutathione / Q000819:agonists / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000378:metabolism; D018763:Muscle Fatigue / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D018384:Oxidative Stress / Q000187:drug effects*; D010805:Physical Conditioning, Animal; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013010:Sonication; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione
keywords: Angelica sinensis polysaccharides; Exhaustive exercise-induced oxidative; Ultrasound-assisted extraction