title: Development of a high-resolution melting marker for selecting Fusarium crown and root rot resistance in tomato.
abstract: Fusarium crown and root rot is a severe fungal disease of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL). In this study, the genomic location of the FORL-resistance locus was determined using a set of molecular markers on chromosome 9 and an F2 population derived from FORL-resistant inbred 'AV107-4' (Solanum lycopersicum) × susceptible 'L3708' (Solanum pimpinellifolium). Bioassay performed using Korean FORL strain KACC 40031 showed single dominant inheritance of FORL resistance in the F2 population. In all, 13 polymerase chain reaction-based markers encompassing approximately 3.6-72.0 Mb of chromosome 9 were developed based on the Tomato-EXPEN 2000 map and SolCAP Tomato single nucleotide polymorphism array analysis. These markers were genotyped on 345 F2 plants, and the FORL-resistance locus was found to be present on a pericentromeric region of suppressed chromosomal recombination in chromosome 9. The location of the FORL-resistance locus was further confirmed by testing these markers against diverse commercial tomato and stock cultivars resistant to FORL. A restriction fragment length polymorphism marker, PNU-D4, located at approximately 6.1 Mb of chromosome 9 showed the highest match with the resistance locus and was used for conducting high-resolution melting analysis for marker-assisted selection of FORL resistance.
authors: a Department of Horticultural Bioscience, Pusan National University, Miryang 627-706, Republic of Korea.
authors: Bichseam
authors: B
authors: Kim
authors: a Department of Horticultural Bioscience, Pusan National University, Miryang 627-706, Republic of Korea.
authors: Nahui
authors: N
authors: Kim
authors: b Department of Plant Science, Gangneung-Wonju National University, Gangneung 210-720, Republic of Korea.
authors: Jun Young
authors: JY
authors: Kim
authors: b Department of Plant Science, Gangneung-Wonju National University, Gangneung 210-720, Republic of Korea.
authors: Byung Sup
authors: BS
authors: Kim
authors: c Department of Horticulture, Sunchun National University, Sunchun 540-950, Republic of Korea.
authors: Hee-Jeong
authors: HJ
authors: Jung
authors: c Department of Horticulture, Sunchun National University, Sunchun 540-950, Republic of Korea.
authors: Indoek
authors: I
authors: Hwang
authors: c Department of Horticulture, Sunchun National University, Sunchun 540-950, Republic of Korea.
authors: Ill-Sup
authors: IS
authors: Noua
authors: d Department of Bioresources Engineering, Sejong University, Seoul 143-747, Republic of Korea.
authors: Sung-Chur
authors: SC
authors: Sim
authors: a Department of Horticultural Bioscience, Pusan National University, Miryang 627-706, Republic of Korea.|e Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Republic of Korea.
authors: Younghoon
authors: Y
authors: Park
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005670:Fusarium; D005819:Genetic Markers; D005838:Genotype; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: Fusarium crown and root rot; Fusarium oxysporum f. sp. radicis-lycopersici; Solanum lycopersicum; disease resistance; marqueur moléculaire; molecular marker; pourriture fusarienne de la tige et des racines; résistance à la maladie