title: Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis.
abstract: Human serum albumin (HSA) is the most abundant plasma protein, endowed with several biological properties unrelated to its oncotic power, such as antioxidant and free-radicals scavenging activities, binding and transport of many endogenous and exogenous substances, and regulation of endothelial function and inflammatory response. These non-oncotic activities are closely connected to the peculiarly dynamic structure of the albumin molecule. HSA undergoes spontaneous structural modifications, mainly by reaction with oxidants and saccharides; however, patients with cirrhosis show extensive post-transcriptional changes at several molecular sites of HSA, the degree of which parallels the severity of the disease. The present work reports the development and application of an innovative LC-MS analytical method for a rapid and reproducible determination of the relative abundance of HSA isoforms in plasma samples from alcoholic hepatitis (AH) patients. A condition of severe oxidative stress, similar to that observed in AH patients, is associated with profound changes in circulating HSA microheterogeneity. More interestingly, the high resolution provided by the analytical platform allowed the monitoring of novel oxidative products of HSA never reported before.
authors: Department of Pharmacy and Biotechnology, Via Sand Donato 15, 40127, University of Bologna, Italy; Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
authors: Marina
authors: M
authors: Naldi
authors: Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
authors: Maurizio
authors: M
authors: Baldassarre
authors: Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
authors: Marco
authors: M
authors: Domenicali
authors: Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
authors: Ferdinando Antonino
authors: FA
authors: Giannone
authors: Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
authors: Matteo
authors: M
authors: Bossi
authors: Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
authors: Jonathan
authors: J
authors: Montomoli
authors: Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
authors: Thomas Damgaard
authors: TD
authors: Sandahl
authors: Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
authors: Emilie
authors: E
authors: Glavind
authors: Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
authors: Hendrik
authors: H
authors: Vilstrup
authors: Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
authors: Paolo
authors: P
authors: Caraceni
authors: Department of Pharmacy and Biotechnology, Via Sand Donato 15, 40127, University of Bologna, Italy. Electronic address: carlo.bertucci@unibo.it.
authors: Carlo
authors: C
authors: Bertucci
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods; D006519:Hepatitis, Alcoholic / Q000097:blood*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D010949:Plasma / Q000737:chemistry*; D020033:Protein Isoforms / Q000097:blood / Q000737:chemistry; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry*; D055815:Young Adult
chemical_list: D020033:Protein Isoforms; D012709:Serum Albumin
keywords: Alcoholic hepatitis; Human serum albumin isoforms; Mass spectrometry; Post-translational modifications