title: Retinoic acid decreases the severity of Salmonella enterica serovar Typhimurium mediated gastroenteritis in a mouse model.
abstract: Gastroenteritis is a global burden; it's the major cause of morbidity and mortality both in adults and children of developing countries. Salmonella is one of the leading causes of bacteria-mediated gastroenteritis and due to its increasing multidrug antibiotic resistance; Salmonella-mediated gastroenteritis is difficult to control. Retinoic acid, the biologically active agent of vitamin A has an anti-inflammatory effect on experimental colitis. In this study we have shown All trans retinoic acid (ATRA) treatment down regulates Salmonella-mediated colitis in a murine model. Macroscopic signs of inflammation such as decrease in body weight and cecum weight, shorter length of proximal colon and pathological score of colitis were observed less in ATRA treated mice than in a vehicle control group. ATRA treatment not only reduced pro-inflammatory cytokine responses, such as TNF-α, IL-6, IL-1β, IFN-γ and IL-17 production but also increased IL-10 response in the supernatant of intestinal tissue. Results also suggested that ATRA treatment enhances the number of FoxP3-expressing T regulatory cells in MLN and also decreases bacterial load in systemic organs. We concluded that ATRA treatment indeed reduces Salmonella Typhimurium-mediated gastroenteritis in mice, suggesting it could be an important part of an alternative therapeutic approach to combat the disease.
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors: Ritam
authors: R
authors: Sinha
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors: Debaki Ranjan
authors: DR
authors: Howlader
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors: Priyadarshini
authors: P
authors: Mukherjee
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors: Sulabh
authors: S
authors: Rai
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India.
authors: Dhrubajyoti
authors: D
authors: Nag
authors: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Kolkata 700010, India. Electronic address: hemantakoley@hotmail.com.
authors: Hemanta
authors: H
authors: Koley
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000378:metabolism; D005759:Gastroenteritis / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012485:Salmonella typhi / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014212:Tretinoin / Q000627:therapeutic use*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D018836:Inflammation Mediators; D014212:Tretinoin
keywords: All trans retinoic acid; FoxP3; Gastroenteritis; Regulatory T cells; Salmonella enterica serover Typhimurium