title: Modification of Titanium Substrates with Chimeric Peptides Comprising Antimicrobial and Titanium-Binding Motifs Connected by Linkers To Inhibit Biofilm Formation.
abstract: Bacterial adhesion and biofilm formation are the primary causes of implant-associated infection, which is difficult to eliminate and may induce failure in dental implants. Chimeric peptides with both binding and antimicrobial motifs may provide a promising alternative to inhibit biofilm formation on titanium surfaces. In this study, chimeric peptides were designed by connecting an antimicrobial motif (JH8194: KRLFRRWQWRMKKY) with a binding motif (minTBP-1: RKLPDA) directly or via flexible/rigid linkers to modify Ti surfaces. We evaluated the binding behavior of peptides using quartz crystal microbalance (QCM) and atomic force microscopy (AFM) techniques and investigated the effect of the modification of titanium surfaces with these peptides on the bioactivity of Streptococcus gordonii (S. gordonii) and Streptococcus sanguis (S. sanguis). Compared with the flexible linker (GGGGS), the rigid linker (PAPAP) significantly increased the adsorption of the chimeric peptide on titanium surfaces (p < 0.05). Concentration-dependent adsorption is consistent with a single Langmuir model, whereas time-dependent adsorption is in line with a two-domain Langmuir model. Additionally, the chimeric peptide with the rigid linker exhibited more effective antimicrobial ability than the peptide with the flexible linker. This finding was ascribed to the ability of the rigid linker to separate functional domains and reduce their interference to the maximum extent. Consequently, the performance of chimeric peptides with specific titanium-binding motifs and antimicrobial motifs against bacteria can be optimized by the proper selection of linkers. This rational design of chimeric peptides provides a promising alternative to inhibit the formation of biofilms on titanium surfaces with the potential to prevent peri-implantitis and peri-implant mucositis.
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Zihao
authors: Z
authors: Liu
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Shiqing
authors: S
authors: Ma
authors: Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029, People's Republic of China.
authors: Shun
authors: S
authors: Duan
authors: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology , Beijing 100081, People's Republic of China.
authors: Deng
authors: D
authors: Xuliang
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Yingchun
authors: Y
authors: Sun
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Xi
authors: X
authors: Zhang
authors: School of Materials Science and Engineering, Tianjin University , Tianjin 300072, People's Republic of China.
authors: Xinhua
authors: X
authors: Xu
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Binbin
authors: B
authors: Guan
authors: School of Materials Science and Engineering, Tianjin University , Tianjin 300072, People's Republic of China.
authors: Chao
authors: C
authors: Wang
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Meilin
authors: M
authors: Hu
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Xingying
authors: X
authors: Qi
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Xu
authors: X
authors: Zhang
authors: School and Hospital of Stomatology, Tianjin Medical University , Tianjin 300070, People's Republic of China.
authors: Ping
authors: P
authors: Gao
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000627:therapeutic use; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000627:therapeutic use*; D015921:Dental Implants / Q000009:adverse effects / Q000382:microbiology*; D006801:Humans; D018625:Microscopy, Atomic Force; D010455:Peptides / Q000737:chemistry* / Q000627:therapeutic use; D058525:Quartz Crystal Microbalance Techniques; D054773:Streptococcus gordonii / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D010455:Peptides; D014025:Titanium
keywords: antimicrobial peptide (AMP); chimeric peptide; surface modification; titanium; titanium-binding peptide