title: Label-free femtomolar cancer biomarker detection in human serum using graphene-coated surface plasmon resonance chips.
abstract: Sensitive and selective detection of cancer biomarkers is vital for the successful diagnosis of early stage cancer and follow-up treatment. Surface Plasmon Resonance (SPR) in combination with different amplification strategies is one of the analytical approaches allowing the screening of protein biomarkers in serum. Here we describe the development of a point-of-care sensor for the detection of folic acid protein (FAP) using graphene-based SPR chips. The exceptional properties of CVD graphene were exploited to construct a highly sensitive and selective SPR chip for folate biomarker sensing in serum. The specific recognition of FAP is based on the interaction between folic acid receptors integrated through π-stacking on the graphene coated SPR chip and the FAP analyte in serum. A simple post-adsorption of human serum:bovine serum albumin (HS:BSA) mixtures onto the folic acid modified sensor resulted in a highly anti-fouling interface, while keeping the sensing capabilities for folate biomarkers. This sensor allowed femtomolar (fM) detection of FAP, a detection limit well adapted and promising for quantitative clinical analysis.
authors: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France; Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
authors: Lijie
authors: L
authors: He
authors: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors: Quentin
authors: Q
authors: Pagneux
authors: SENSIA SL, Poligono Aranguren, 9, Apdo, Correos 171, 20180 Oiartzun, Gipuzkoa, Spain.
authors: Iban
authors: I
authors: Larroulet
authors: SENSIA SL, Poligono Aranguren, 9, Apdo, Correos 171, 20180 Oiartzun, Gipuzkoa, Spain.
authors: Aritz Yanguas
authors: AY
authors: Serrano
authors: Graphenea S.A., Tolosa Hiribidea, 76, 20018 Donostia, San Sebastian, Spain.
authors: Amaia
authors: A
authors: Pesquera
authors: Graphenea S.A., Tolosa Hiribidea, 76, 20018 Donostia, San Sebastian, Spain.
authors: Amaia
authors: A
authors: Zurutuza
authors: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
authors: Daniel
authors: D
authors: Mandler
authors: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors: Rabah
authors: R
authors: Boukherroub
authors: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille1 University, Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France. Electronic address: sabine.szunerits@iri.univ-lille1.fr.
authors: Sabine
authors: S
authors: Szunerits
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D058973:Folate Receptors, GPI-Anchored / Q000032:analysis / Q000097:blood*; D005492:Folic Acid / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008958:Models, Molecular; D019095:Point-of-Care Systems; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D014408:Biomarkers, Tumor; D058973:Folate Receptors, GPI-Anchored; D006108:Graphite; D005492:Folic Acid
keywords: Biomarker; Folate receptor; Folic acid; Folic acid protein; Graphene; SPR