title: Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses.
abstract: Aiming to enhance the immunogenicity of subunit vaccines, a novel antigen delivery and adjuvant system based on dopamine polymerization on the surface of poly(D,L-lactic-glycolic-acid) nanoparticles (NPs) with multiple mechanisms of immunity enhancement is developed. The mussel-inspired biomimetic polydopamine (pD) not only serves as a coating to NPs but also functionalizes NP surfaces. The method is facile and mild including simple incubation of the preformed NPs in the weak alkaline dopamine solution, and incorporation of hepatitis B surface antigen and TLR9 agonist unmethylated cytosine-guanine (CpG) motif with the pD surface. The as-constructed NPs possess pathogen-mimicking manners owing to their size, shape, and surface molecular immune-activating properties given by CpG. The biocompatibility and biosafety of these pathogen-mimicking NPs are confirmed using bone marrow-derived dendritic cells. Pathogen-mimicking NPs hold great potential as vaccine delivery and adjuvant system due to their ability to: 1) enhance cytokine secretion and immune cell recruitment at the injection site; 2) significantly activate and maturate dendritic cells; 3) induce stronger humoral and cellular immune responses in vivo. Furthermore, this simple and versatile dopamine polymerization method can be applicable to endow NPs with characteristics to mimic pathogen structure and function, and manipulate NPs for the generation of efficacious vaccine adjuvants.
authors: National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors: Qi
authors: Q
authors: Liu
authors: National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors: Jilei
authors: J
authors: Jia
authors: National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
authors: Tingyuan
authors: T
authors: Yang
authors: National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors: Qingze
authors: Q
authors: Fan
authors: National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
authors: Lianyan
authors: L
authors: Wang
authors: National Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, P. R. China.
authors: Guanghui
authors: G
authors: Ma
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism; D004298:Dopamine / Q000737:chemistry*; D020869:Gene Expression Profiling; D007111:Immunity, Cellular / Q000187:drug effects*; D056724:Immunity, Humoral / Q000187:drug effects*; D007114:Immunization; D007211:Indoles / Q000737:chemistry*; D007267:Injections; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D013154:Spleen / Q000166:cytology; D055672:Static Electricity; D013601:T-Lymphocytes / Q000276:immunology; D014612:Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D018925:Chemokines; D007211:Indoles; D009838:Oligodeoxyribonucleotides; D011108:Polymers; D014612:Vaccines; C568283:polydopamine; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004298:Dopamine
keywords: dopamine polymerization; pathogen-mimicking; polymeric nanoparticles; surface modification; vaccine delivery and adjuvant systems