title: Quorum sensing activity of Citrobacter amalonaticus L8A, a bacterium isolated from dental plaque.
abstract: Cell-cell communication is also known as quorum sensing (QS) that happens in the bacterial cells with the aim to regulate their genes expression in response to increased cell density. In this study, a bacterium (L8A) isolated from dental plaque biofilm was identified as Citrobacter amalonaticus by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Its N-acylhomoserine-lactone (AHL) production was screened by using two types of AHL biosensors namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Citrobacter amalonaticus strain L8A was identified and confirmed producing numerous types of AHL namely N-butyryl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL) and N-hexadecanoyl-L-homoserine lactone (C16-HSL). We performed the whole genome sequence analysis of this oral isolate where its genome sequence reveals the presence of QS signal synthase gene and our work will pave the ways to study the function of the related QS genes in this bacterium.
authors: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Share-Yuan
authors: SY
authors: Goh
authors: Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Saad Ahmed
authors: SA
authors: Khan
authors: Department of Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Kok Keng
authors: KK
authors: Tee
authors: Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Noor Hayaty
authors: NH
authors: Abu Kasim
authors: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Wai-Fong
authors: WF
authors: Yin
authors: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Kok-Gan
authors: KG
authors: Chan
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D002954:Citrobacter / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003773:Dental Plaque / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D006714:Homoserine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007783:Lactones / Q000032:analysis / Q000378:metabolism; D010802:Phylogeny; D053038:Quorum Sensing / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007783:Lactones; C092312:N-butyrylhomoserine lactone; C104908:N-hexanoyl-L-homoserine lactone; C091088:N-octanoylhomoserine lactone; C088386:homoserine lactone; D006714:Homoserine; D015107:4-Butyrolactone