title: Fructose consumption does not worsen bone deficits resulting from high-fat feeding in young male rats.
abstract: Dietary-induced obesity (DIO) resulting from high-fat (HF) or high-sugar diets produces a host of deleterious metabolic consequences including adverse bone development. We compared the effects of feeding standard rodent chow (Control), a 30% moderately HF (starch-based/sugar-free) diet, or a combined 30%/40% HF/high-fructose (HF/F) diet for 12weeks on cancellous/cortical bone development in male Sprague-Dawley rats aged 8weeks. Both HF feeding regimens reduced the lean/fat mass ratio, elevated circulating leptin, and reduced serum total antioxidant capacity (tAOC) when compared with Controls. Distal femur cancellous bone mineral density (BMD) was 23-34% lower in both HF groups (p<0.001) and was characterized by lower cancellous bone volume (BV/TV, p<0.01), lower trabecular number (Tb.N, p<0.001), and increased trabecular separation versus Controls (p<0.001). Cancellous BMD, BV/TV, and Tb.N were negatively associated with leptin and positively associated with tAOC at the distal femur. Similar cancellous bone deficits were observed at the proximal tibia, along with increased bone marrow adipocyte density (p<0.05), which was negatively associated with BV/TV and Tb.N. HF/F animals also exhibited lower osteoblast surface and reduced circulating osteocalcin (p<0.05). Cortical thickness (p<0.01) and tissue mineral density (p<0.05) were higher in both HF-fed groups versus Controls, while whole bone biomechanical characteristics were not different among groups. These results demonstrate that "westernized" HF diets worsen cancellous, but not cortical, bone parameters in skeletally-immature male rats and that fructose incorporation into HF diets does not exacerbate bone loss. In addition, they suggest that leptin and/or oxidative stress may influence DIO-induced alterations in adolescent bone development.
authors: Research Service, Malcom Randall Department of Veterans Affairs Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL 32608, USA; Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL 32611, USA. Electronic address: jfyarrow@ufl.edu.
authors: Joshua F
authors: JF
authors: Yarrow
authors: Geriatric Research, Education, and Clinical Center (GRECC), Malcom Randall Department of Veterans Affairs Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL 32608, USA; Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32611, USA.
authors: Hale Z
authors: HZ
authors: Toklu
authors: Research Service, Malcom Randall Department of Veterans Affairs Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL 32608, USA.
authors: Alex
authors: A
authors: Balaez
authors: Research Service, Malcom Randall Department of Veterans Affairs Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL 32608, USA.
authors: Ean G
authors: EG
authors: Phillips
authors: Geriatric Research, Education, and Clinical Center (GRECC), Malcom Randall Department of Veterans Affairs Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL 32608, USA.
authors: Dana M
authors: DM
authors: Otzel
authors: Department of Orthopaedics and Rehabilitation, University of Florida, Gainesville, FL 32611, USA.
authors: Cong
authors: C
authors: Chen
authors: Department of Physiological Sciences, University of Florida, Gainesville, FL 32611, USA.
authors: Thomas J
authors: TJ
authors: Wronski
authors: Department of Physiological Sciences, University of Florida, Gainesville, FL 32611, USA.
authors: J Ignacio
authors: JI
authors: Aguirre
authors: Geriatric Research, Education, and Clinical Center (GRECC), Malcom Randall Department of Veterans Affairs Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL 32608, USA; Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32611, USA.
authors: Yasemin
authors: Y
authors: Sakarya
authors: Geriatric Research, Education, and Clinical Center (GRECC), Malcom Randall Department of Veterans Affairs Medical Center, North Florida/South Georgia Veterans Health System, Gainesville, FL 32608, USA; Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32611, USA.
authors: Nihal
authors: N
authors: Tümer
authors: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL 32611, USA.
authors: Philip J
authors: PJ
authors: Scarpace
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000473:pathology; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001823:Body Composition / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D000071556:Cancellous Bone / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D059305:Diet, High-Fat; D005247:Feeding Behavior; D005632:Fructose / Q000494:pharmacology*; D008297:Male; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D005632:Fructose
keywords: Adipose; Adiposity; Diet; Fat; Osteoporosis; Sugar