title: Gold-Quantum Dot Core-Satellite Assemblies for Lighting Up MicroRNA In Vitro and In Vivo.
abstract: A high yield DNA-driven gold-quantum dot core-satellite is developed for miRNA detection in vitro and vivo. In the presence of the target miRNA, the DNA hairpin between core and satellite is ruined, resulting in the recovery of fluorescence. The limit of detection for miRNA-21 detection in living cells reaches 296 copies per cell.
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors: Xueli
authors: X
authors: Zhao
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors: Liguang
authors: L
authors: Xu
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors: Maozhong
authors: M
authors: Sun
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors: Wei
authors: W
authors: Ma
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors: Xiaoling
authors: X
authors: Wu
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China. kuangh@jiangnan.edu.cn.
authors: Hua
authors: H
authors: Kuang
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors: Libing
authors: L
authors: Wang
authors: State Key Lab of Food Science and Technology, International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.
authors: Chuanlai
authors: C
authors: Xu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D006046:Gold
keywords: core-satellite assemblies; detection; fluorescence; microRNA; self-assembly