title: A mitochondrial-targeted ubiquinone modulates muscle lipid profile and improves mitochondrial respiration in obesogenic diet-fed rats.
abstract: The prevalence of the metabolic syndrome components including abdominal obesity, dyslipidaemia and insulin resistance is increasing in both developed and developing countries. It is generally accepted that the development of these features is preceded by, or accompanied with, impaired mitochondrial function. The present study was designed to analyse the effects of a mitochondrial-targeted lipophilic ubiquinone (MitoQ) on muscle lipid profile modulation and mitochondrial function in obesogenic diet-fed rats. For this purpose, twenty-four young male Sprague-Dawley rats were divided into three groups and fed one of the following diets: (1) control, (2) high fat (HF) and (3) HF+MitoQ. After 8 weeks, mitochondrial function markers and lipid metabolism/profile modifications in skeletal muscle were measured. The HF diet was effective at inducing the major features of the metabolic syndrome--namely, obesity, hepatic enlargement and glucose intolerance. MitoQ intake prevented the increase in rat body weight, attenuated the increase in adipose tissue and liver weights and partially reversed glucose intolerance. At the muscle level, the HF diet induced moderate TAG accumulation associated with important modifications in the muscle phospholipid classes and in the fatty acid composition of total muscle lipid. These lipid modifications were accompanied with decrease in mitochondrial respiration. MitoQ intake corrected the lipid alterations and restored mitochondrial respiration. These results indicate that MitoQ protected obesogenic diet-fed rats from some features of the metabolic syndrome through its effects on muscle lipid metabolism and mitochondrial activity. These findings suggest that MitoQ is a promising candidate for future human trials in the metabolic syndrome prevention.
authors: 1UMR 866 (Muscular Dynamic & Metabolism),INRA,Place Viala,34060 Montpellier,France.
authors: Charles
authors: C
authors: Coudray
authors: 1UMR 866 (Muscular Dynamic & Metabolism),INRA,Place Viala,34060 Montpellier,France.
authors: Gilles
authors: G
authors: Fouret
authors: 2U1046 INSERM,UMR9214 CNRS,Université de Montpellier,34295 Montpellier,France.
authors: Karen
authors: K
authors: Lambert
authors: 3Istituto per la Sintesi Organica e la Fotoreattività (ISOF) Bio Free Radicals,40129 Bologna,Italy.
authors: Carla
authors: C
authors: Ferreri
authors: 4UMR U1060, INSERM/Faculté de médecine Lyon-Sud,69921 Oullins,France.
authors: Jennifer
authors: J
authors: Rieusset
authors: 5Department of Physiology,Medical University,Mickiewicza 2c,15-222 Bialystok,Poland.
authors: Agnieszka
authors: A
authors: Blachnio-Zabielska
authors: 6Centre de recherche agronomique pour le développement (CIRAD)/SupAgro,UMR IATE,F-34398 Montpellier,France.
authors: Jérôme
authors: J
authors: Lecomte
authors: 2U1046 INSERM,UMR9214 CNRS,Université de Montpellier,34295 Montpellier,France.
authors: Raymond
authors: R
authors: Ebabe Elle
authors: 2U1046 INSERM,UMR9214 CNRS,Université de Montpellier,34295 Montpellier,France.
authors: Eric
authors: E
authors: Badia
authors: 7Medical Research Council Mitochondrial Biology Unit,Hills Road,Cambridge CB2 0XY,UK.
authors: Michael P
authors: MP
authors: Murphy
authors: 1UMR 866 (Muscular Dynamic & Metabolism),INRA,Place Viala,34060 Montpellier,France.
authors: Christine
authors: C
authors: Feillet-Coudray
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000818:Animals; D059305:Diet, High-Fat; D005227:Fatty Acids / Q000032:analysis; D018149:Glucose Intolerance / Q000517:prevention & control; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000473:pathology; D008297:Male; D024821:Metabolic Syndrome / Q000517:prevention & control; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D009765:Obesity / Q000209:etiology / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology; D015430:Weight Gain / Q000187:drug effects
chemical_list: D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D014451:Ubiquinone
keywords: DAG diacylglycerol; Fatty acids; HFD high-fat diet; High-fat diet; IR insulin resistance; Metabolic syndrome; MitoQ; MitoQ mitochondrial-targeted lipophilic ubiquinone; Mitochondria; Muscle; PC phosphatidylcholine; Phospholipids; SM sphingomyelin