title: Effect of the chelation of metal cation on the antioxidant activity of chondroitin sulfates.
abstract: The antioxidant potencies of chondroitin sulfates (CSs) from shark cartilage, salmon cartilage, bovine trachea, and porcine intestinal mucosa were compared by three representative methods for the measurement of the antioxidant activity; DPPH radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging activity. CSs from salmon cartilage and bovine trachea showed higher potency in comparison with CSs from shark cartilage and porcine intestinal mucosa. Next, CS from salmon cartilage chelating with Ca(2+), Mg(2+), Mn(2+), or Zn(2+) were prepared, and their antioxidant potencies were compared. CS chelating with Ca(2+) or Mg(2+) ions showed rather decreased DPPH radical scavenging activity in comparison with CS of H(+) form. In contrast, CS chelating with Ca(2+) or Mg(2+) ion showed remarkably enhanced superoxide radical scavenging activity than CS of H(+) or Na(+) form. Moreover, CS chelating with divalent metal ions, Ca(2+), Mg(2+), Mn(2+), or Zn(2+), showed noticeably higher hydroxyl radical scavenging activity than CS of H(+) or Na(+) form. The present results revealed that the scavenging activities of, at least, superoxide radical and hydroxyl radical were enhanced by the chelation with divalent metal ions.
authors: a Department of Food Science , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Katsumi
authors: K
authors: Ajisaka
authors: a Department of Food Science , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Yutaka
authors: Y
authors: Oyanagi
authors: a Department of Food Science , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Tatsuo
authors: T
authors: Miyazaki
authors: a Department of Food Science , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Yasuhiro
authors: Y
authors: Suzuki
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors* / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D002356:Cartilage / Q000737:chemistry; D002413:Cations, Divalent; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry* / Q000302:isolation & purification; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors* / Q000737:chemistry; D007413:Intestinal Mucosa / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D010851:Picrates / Q000037:antagonists & inhibitors* / Q000737:chemistry; D012487:Salmonidae; D012754:Sharks; D013481:Superoxides / Q000037:antagonists & inhibitors* / Q000737:chemistry; D013552:Swine; D014132:Trachea / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002413:Cations, Divalent; D002614:Chelating Agents; D010851:Picrates; D013481:Superoxides; D017665:Hydroxyl Radical; D008345:Manganese; D002809:Chondroitin Sulfates; C004931:1,1-diphenyl-2-picrylhydrazyl; D008274:Magnesium; D015032:Zinc; D002118:Calcium
keywords: antioxidant activity; chelation; chondroitin sulfate; metal ion