title: Interleukin-1Ra rs2234663 and Interleukin-4 rs79071878 Polymorphisms in Familial Mediterranean Fever.
abstract: OBJECTIVE
  Familial Mediterranean Fever (FMF) is an autosomal recessively inherited auto inflammatory disorder. MEFV gene, causing FMF, encodes pyrin that is associated with the interleukin-1 (IL-1) related inflammation cascade. The aim of this study was to investigate the relationship of interleukin-1 receptor antagonist (IL-1Ra) and interleukin-4 (IL-4) polymorphisms with the risk of FMF in the Turkish population.
  
  
  METHODS
  This study included 160 patients with FMF (74 men, 86 women) and 120 healthy controls (50 men, 70 women), respectively. Genotyping of IL-1Ra rs2234663 polymorphism was evaluated by gel electrophoresis after polymerase chain reaction (PCR). The IL-4 rs79071878 polymorphism was determined by PCR-based restriction fragment length polymorphism (PCR-RFLP) analysis. The results of analyses were evaluated for statistical significance.
  
  
  RESULTS
  There was no significant difference in IL-1Ra genotype and allele distributions between FMF and the control groups (p>0.05). However, a significant association was observed between FMF patients and control groups according to IL-4 genotype distribution (p=0.016), but no association was found in the allelic frequency of IL-4 between FMF patients and the controls (p>0.05, OR: 1.131, CI 95%: 0.71-1.81).
  
  
  CONCLUSIONS
  The IL-4 rs79071878 polymorphism, was associated whereas the IL-1Ra rs2234663 polymorphism was not associated with FMF risk in the Turkish population. Larger studies with different ethnicities are needed to determine the impact of IL-1Ra and IL-4 polymorphism on the risk of developing FMF.
authors: Giresun University, Faculty of Medicine, Department of Medical Genetic, Giresun, Turkey.
authors: Ayse Feyda
authors: AF
authors: Nursal
authors: Ahi Evran University, School of Health, Kirsehir, Turkey. Electronic address: akintekcan@hotmail.com.
authors: Akin
authors: A
authors: Tekcan
authors: Gaziosmanpasa University, Faculty of Medicine, Department of Internal Medicine, Tokat, Turkey.
authors: Suheyla Uzun
authors: SU
authors: Kaya
authors: Samsun Training and Research Hospital, Genetic Clinics, Samsun, Turkey.
authors: Ozlem
authors: O
authors: Sezer
authors: Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors: Serbulent
authors: S
authors: Yigit
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D003710:Demography; D010505:Familial Mediterranean Fever / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: C508598:IL4 protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D015847:Interleukin-4
keywords: Familial Mediterranean Fever; IL-1Ra; IL-4; Polymorphism