title: Simultaneous quantification of neuroactive dopamine serotonin and kynurenine pathway metabolites in gender-specific youth urine by ultra performance liquid chromatography tandem high resolution mass spectrometry.
abstract: Neuroactive metabolites in dopamine, serotonin and kynurenine metabolic pathways play key roles in several physiological processes and their imbalances have been implicated in the pathophysiology of a wide range of disorders. The association of these metabolites' alterations with various pathologies has raised interest in analytical methods for accurate quantification in biological fluids. However, simultaneous measurement of various neuroactive metabolites represents great challenges due to their trace level, high polarity and instability. In this study, an analytical method was developed and validated for accurately quantifying 12 neuroactive metabolites covering three metabolic pathways in youth urine by ultra performance liquid chromatography coupled to electrospray tandem high resolution mass spectrometry (UPLC-ESI-HRMS/MS). The strategy of dansyl chloride derivatization followed by solid phase extraction on C18 cartridges were employed to reduce matrix interference and improve the extraction efficiency. The reverse phase chromatographic separation was achieved with a gradient elution program in 20 min. The high resolution mass spectrometer (Q Exactive) was employed, with confirmation and quantification by Target-MS/MS scan mode. Youth urine samples collected from 100 healthy volunteers (Female:Male=1:1) were analyzed to explore the differences in metabolite profile and their turnover between genders. The results demonstrated that the UPLC-ESI-HRMS/MS method is sensitive and robust, suitable for monitoring a large panel of metabolites and for discovering new biomarkers in the medical fields.
authors: Department of Hygienic Analysis and Detection, Key Lab of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing 211166, China.
authors: Haihua
authors: H
authors: Lu
authors: Department of Hygienic Analysis and Detection, Key Lab of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing 211166, China.
authors: Jing
authors: J
authors: Yu
authors: Department of Toxicology, Key Lab of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing 211166, China.
authors: Jun
authors: J
authors: Wang
authors: Department of Hygienic Analysis and Detection, Key Lab of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing 211166, China.
authors: Linlin
authors: L
authors: Wu
authors: Department of Toxicology, Key Lab of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing 211166, China.
authors: Hang
authors: H
authors: Xiao
authors: Department of Hygienic Analysis and Detection, Key Lab of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, 818 Tianyuan East Road, Nanjing 211166, China. Electronic address: gaorong@njmu.edu.cn.
authors: Rong
authors: R
authors: Gao
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000737:chemistry / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D005260:Female; D006801:Humans; D007737:Kynurenine / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D008297:Male; D012701:Serotonin / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014556:Urine / Q000737:chemistry*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D012701:Serotonin; D007737:Kynurenine; D004298:Dopamine
keywords: Dansyl chloride derivatization; Neuroactive metabolites; Sex difference; Solid phase extraction; UPLC-ESI-HRMS/MS; Urine