title: De Novo Assembled Wheat Transcriptomes Delineate Differentially Expressed Host Genes in Response to Leaf Rust Infection.
abstract: Pathogens like Puccinia triticina, the causal organism for leaf rust, extensively damages wheat production. The interaction at molecular level between wheat and the pathogen is complex and less explored. The pathogen induced response was characterized using mock- or pathogen inoculated near-isogenic wheat lines (with or without seedling leaf rust resistance gene Lr28). Four Serial Analysis of Gene Expression libraries were prepared from mock- and pathogen inoculated plants and were subjected to Sequencing by Oligonucleotide Ligation and Detection, which generated a total of 165,767,777 reads, each 35 bases long. The reads were processed and multiple k-mers were attempted for de novo transcript assembly; 22 k-mers showed the best results. Altogether 21,345 contigs were generated and functionally characterized by gene ontology annotation, mining for transcription factors and resistance genes. Expression analysis among the four libraries showed extensive alterations in the transcriptome in response to pathogen infection, reflecting reorganizations in major biological processes and metabolic pathways. Role of auxin in determining pathogenesis in susceptible and resistant lines were imperative. The qPCR expression study of four LRR-RLK (Leucine-rich repeat receptor-like protein kinases) genes showed higher expression at 24 hrs after inoculation with pathogen. In summary, the conceptual model of induced resistance in wheat contributes insights on defense responses and imparts knowledge of Puccinia triticina-induced defense transcripts in wheat plants.
authors: Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215 Jharkhand, India.
authors: Saket
authors: S
authors: Chandra
authors: Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215 Jharkhand, India.
authors: Dharmendra
authors: D
authors: Singh
authors: Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215 Jharkhand, India.
authors: Jyoti
authors: J
authors: Pathak
authors: Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut 200005, Uttar Pradesh, India.
authors: Supriya
authors: S
authors: Kumari
authors: Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215 Jharkhand, India.
authors: Manish
authors: M
authors: Kumar
authors: Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215 Jharkhand, India.
authors: Raju
authors: R
authors: Poddar
authors: Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut 200005, Uttar Pradesh, India.
authors: Harindra Singh
authors: HS
authors: Balyan
authors: Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut 200005, Uttar Pradesh, India.
authors: Puspendra Kumar
authors: PK
authors: Gupta
authors: Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India.
authors: Kumble Vinod
authors: KV
authors: Prabhu
authors: Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215 Jharkhand, India.
authors: Kunal
authors: K
authors: Mukhopadhyay
mesh_terms: D001487:Basidiomycota / Q000254:growth & development*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D036226:Seedlings / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D014908:Triticum / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology