title: Quantifying the cooperative subunit action in a multimeric membrane receptor.
abstract: In multimeric membrane receptors the cooperative action of the subunits prevents exact knowledge about the operation and the interaction of the individual subunits. We propose a method that permits quantification of ligand binding to and activation effects of the individual binding sites in a multimeric membrane receptor. The power of this method is demonstrated by gaining detailed insight into the subunit action in olfactory cyclic nucleotide-gated CNGA2 ion channels.
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
authors: Nisa
authors: N
authors: Wongsamitkul
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
authors: Vasilica
authors: V
authors: Nache
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
authors: Thomas
authors: T
authors: Eick
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.|Hochschule Schmalkalden, Fakultät Elektrotechnik, Blechhammer, 98574 Schmalkalden, Germany.
authors: Sabine
authors: S
authors: Hummert
authors: Hochschule Schmalkalden, Fakultät Elektrotechnik, Blechhammer, 98574 Schmalkalden, Germany.
authors: Eckhard
authors: E
authors: Schulz
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
authors: Ralf
authors: R
authors: Schmauder
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
authors: Jana
authors: J
authors: Schirmeyer
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
authors: Thomas
authors: T
authors: Zimmer
authors: Institut für Physiologie II, Universitätsklinikum Jena, Friedrich-Schiller-Universität Jena, 07743 Jena, Germany.
authors: Klaus
authors: K
authors: Benndorf
mesh_terms: D000818:Animals; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008024:Ligands; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D014982:Xenopus laevis
chemical_list: C518239:Cnga2 protein, rat; D054815:Cyclic Nucleotide-Gated Cation Channels; D008024:Ligands