title: Spatio-Temporal Detection of the Thiomonas Population and the Thiomonas Arsenite Oxidase Involved in Natural Arsenite Attenuation Processes in the Carnoulès Acid Mine Drainage.
abstract: The acid mine drainage (AMD) impacted creek of the Carnoulès mine (Southern France) is characterized by acid waters with a high heavy metal content. The microbial community inhabiting this AMD was extensively studied using isolation, metagenomic and metaproteomic methods, and the results showed that a natural arsenic (and iron) attenuation process involving the arsenite oxidase activity of several Thiomonas strains occurs at this site. A sensitive quantitative Selected Reaction Monitoring (SRM)-based proteomic approach was developed for detecting and quantifying the two subunits of the arsenite oxidase and RpoA of two different Thiomonas groups. Using this approach combined with FISH and pyrosequencing-based 16S rRNA gene sequence analysis, it was established here for the first time that these Thiomonas strains are ubiquitously present in minor proportions in this AMD and that they express the key enzymes involved in natural remediation processes at various locations and time points. In addition to these findings, this study also confirms that targeted proteomics applied at the community level can be used to detect weakly abundant proteins in situ.
authors: Laboratoire de Spectrométrie de Masse BioOrganique, Institut Pluridisciplinaire Hubert Curien, UMR7178, Centre National de la Recherche Scientifique, Université de Strasbourg Strasbourg, France.
authors: Agnès
authors: A
authors: Hovasse
authors: Laboratoire HydroSciences Montpellier, UMR HSM 5569, Centre National de la Recherche Scientifique, Institut de Recherche pour le Développement, Université Montpellier Montpellier, France.
authors: Odile
authors: O
authors: Bruneel
authors: Laboratoire HydroSciences Montpellier, UMR HSM 5569, Centre National de la Recherche Scientifique, Institut de Recherche pour le Développement, Université Montpellier Montpellier, France.
authors: Corinne
authors: C
authors: Casiot
authors: Laboratoire HydroSciences Montpellier, UMR HSM 5569, Centre National de la Recherche Scientifique, Institut de Recherche pour le Développement, Université Montpellier Montpellier, France.
authors: Angélique
authors: A
authors: Desoeuvre
authors: Laboratoire Génétique Moléculaire, Génomique et Microbiologie, UMR7156, Centre National de la Recherche Scientifique-Université de Strasbourg, Département Microorganismes, Génomes, Environnement, Equipe Ecophysiologie Moléculaire des Microorganismes Strasbourg, France.
authors: Julien
authors: J
authors: Farasin
authors: Laboratoire HydroSciences Montpellier, UMR HSM 5569, Centre National de la Recherche Scientifique, Institut de Recherche pour le Développement, Université Montpellier Montpellier, France.
authors: Marina
authors: M
authors: Hery
authors: Laboratoire de Spectrométrie de Masse BioOrganique, Institut Pluridisciplinaire Hubert Curien, UMR7178, Centre National de la Recherche Scientifique, Université de Strasbourg Strasbourg, France.
authors: Alain
authors: A
authors: Van Dorsselaer
authors: Laboratoire de Spectrométrie de Masse BioOrganique, Institut Pluridisciplinaire Hubert Curien, UMR7178, Centre National de la Recherche Scientifique, Université de Strasbourg Strasbourg, France.
authors: Christine
authors: C
authors: Carapito
authors: Laboratoire Génétique Moléculaire, Génomique et Microbiologie, UMR7156, Centre National de la Recherche Scientifique-Université de Strasbourg, Département Microorganismes, Génomes, Environnement, Equipe Ecophysiologie Moléculaire des Microorganismes Strasbourg, France.
authors: Florence
authors: F
authors: Arsène-Ploetze
keywords: FISH; LC-SRM; acid mine drainage; community proteomics; pyrosequencing; targeted proteomics