title: Polymorphisms in pre-miRNA genes and cooking oil fume exposure as well as their interaction on the risk of lung cancer in a Chinese nonsmoking female population.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are suggested to be very important in the development of lung cancer. This study assesses the association between polymorphisms in miRNA-related (miR)-26a-1, miR-605, and miR-16-1 genes and risk of lung cancer, as well as the effect of gene-environment interaction between miRNA polymorphisms and cooking fume exposure on lung cancer.
  
  
  METHODS
  A case-control study including 268 diagnosed nonsmoking female lung cancer patients and 266 nonsmoking female controls was carried out. Three miRNA polymorphisms (miR-26a-1 rs7372209, miR-605 rs2043556, and miR-16-1 rs1022960) were analyzed. Both additive and multiplicative interactions were assessed.
  
  
  RESULTS
  MiR-16-1 rs1022960 may be associated with the risk of lung cancer. Carriers with TT genotype of miR-16-1 rs1022960 were observed to have a decreased risk of lung cancer compared with CC and CT genotype carriers (odds ratio =0.550, 95% confidence interval =0.308-0.983, P=0.044). MiR-26a-1 rs7372209 and miR-605 rs2043556 showed no statistically significant associations with lung cancer risk. There were no significant associations between the three single nucleotide polymorphisms and lung adenocarcinoma. People with exposure to both risk genotypes of miR-26a-1 rs7372209 and cooking oil fumes were more likely to develop lung cancer than those with only genetic risk factor or cooking oil fumes (odds ratios were 2.136, 1.255, and 1.730, respectively). The measures of biological interaction and logistic models indicate that gene-environment interactions were not statistically significant on additive scale or multiplicative scale.
  
  
  CONCLUSIONS
  MiR-16-1 rs1022960 may be associated with the risk of lung cancer in a Chinese nonsmoking female population. The interactions between miRNA polymorphisms (miR-26a-1 rs7372209, miR-605 rs2043556, and miR-16-1 rs1022960) and cooking oil fumes were not statistically significant.
authors: Department of Epidemiology, School of Public Health, China Medical University, Shenyang, People's Republic of China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, People's Republic of China.
authors: Zhihua
authors: Z
authors: Yin
authors: Department of Epidemiology, School of Public Health, China Medical University, Shenyang, People's Republic of China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, People's Republic of China.
authors: Hang
authors: H
authors: Li
authors: China Medical University, Shenyang, People's Republic of China.
authors: Zhigang
authors: Z
authors: Cui
authors: Department of Epidemiology, School of Public Health, China Medical University, Shenyang, People's Republic of China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, People's Republic of China.
authors: Yangwu
authors: Y
authors: Ren
authors: Department of Epidemiology, School of Public Health, China Medical University, Shenyang, People's Republic of China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, People's Republic of China.
authors: Xuelian
authors: X
authors: Li
authors: Department of Epidemiology, School of Public Health, China Medical University, Shenyang, People's Republic of China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, People's Republic of China.
authors: Wei
authors: W
authors: Wu
authors: Department of Epidemiology, School of Public Health, China Medical University, Shenyang, People's Republic of China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, People's Republic of China.
authors: Peng
authors: P
authors: Guan
authors: Department of Epidemiology, School of Public Health, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors: Biyun
authors: B
authors: Qian
authors: Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA.
authors: Nathaniel
authors: N
authors: Rothman
authors: Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA.
authors: Qing
authors: Q
authors: Lan
authors: Department of Epidemiology, School of Public Health, China Medical University, Shenyang, People's Republic of China; Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, Shenyang, People's Republic of China.
authors: Baosen
authors: B
authors: Zhou
keywords: cooking oil fume; interaction; lung cancer; microRNA; single nucleotide polymorphism