title: Tuft cells, taste-chemosensory cells, orchestrate parasite type 2 immunity in the gut.
abstract: The intestinal epithelium forms an essential barrier between a host and its microbiota. Protozoa and helminths are members of the gut microbiota of mammals, including humans, yet the many ways that gut epithelial cells orchestrate responses to these eukaryotes remain unclear. Here we show that tuft cells, which are taste-chemosensory epithelial cells, accumulate during parasite colonization and infection. Disruption of chemosensory signaling through the loss of TRMP5 abrogates the expansion of tuft cells, goblet cells, eosinophils, and type 2 innate lymphoid cells during parasite colonization. Tuft cells are the primary source of the parasite-induced cytokine interleukin-25, which indirectly induces tuft cell expansion by promoting interleukin-13 production by innate lymphoid cells. Our results identify intestinal tuft cells as critical sentinels in the gut epithelium that promote type 2 immunity in response to intestinal parasites.
authors: Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard T. H. Chan School of Public Health, Boston, MA 02115, USA.
authors: Michael R
authors: MR
authors: Howitt
authors: Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard T. H. Chan School of Public Health, Boston, MA 02115, USA.
authors: Sydney
authors: S
authors: Lavoie
authors: Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard T. H. Chan School of Public Health, Boston, MA 02115, USA.
authors: Monia
authors: M
authors: Michaud
authors: Division of Gastroenterology, Tufts Medical Center, Boston, MA 02111, USA.
authors: Arthur M
authors: AM
authors: Blum
authors: Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
authors: Sara V
authors: SV
authors: Tran
authors: Division of Gastroenterology, Tufts Medical Center, Boston, MA 02111, USA.
authors: Joel V
authors: JV
authors: Weinstock
authors: Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard T. H. Chan School of Public Health, Boston, MA 02115, USA.
authors: Carey Ann
authors: CA
authors: Gallini
authors: Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
authors: Kevin
authors: K
authors: Redding
authors: Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
authors: Robert F
authors: RF
authors: Margolskee
authors: Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medical College, Cornell University, New York, NY 10021, USA.
authors: Lisa C
authors: LC
authors: Osborne
authors: Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Medical College, Cornell University, New York, NY 10021, USA.
authors: David
authors: D
authors: Artis
authors: Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard T. H. Chan School of Public Health, Boston, MA 02115, USA. Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA 02142, USA. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA. wgarrett@hsph.harvard.edu.
authors: Wendy S
authors: WS
authors: Garrett
mesh_terms: D000818:Animals; D002628:Chemoreceptor Cells / Q000276:immunology*; D000089742:Doublecortin-Like Kinases; D004804:Eosinophils / Q000276:immunology; D020397:Goblet Cells / Q000276:immunology; D006373:Helminthiasis / Q000276:immunology / Q000469:parasitology; D006376:Helminths / Q000276:immunology; D018928:Immunity, Mucosal; D018793:Interleukin-13 / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology; D007411:Intestinal Diseases, Parasitic / Q000276:immunology* / Q000469:parasitology; D007413:Intestinal Mucosa / Q000276:immunology* / Q000469:parasitology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D064307:Microbiota / Q000276:immunology*; D017346:Protein Serine-Threonine Kinases / Q000276:immunology; D011528:Protozoan Infections / Q000276:immunology / Q000469:parasitology; D015398:Signal Transduction; D050053:TRPM Cation Channels / Q000276:immunology*; D013649:Taste; D015293:Transducin / Q000235:genetics / Q000276:immunology; D014318:Tritrichomonas / Q000276:immunology
chemical_list: D018793:Interleukin-13; D020381:Interleukin-17; D050053:TRPM Cation Channels; C498617:Trpm5 protein, mouse; C075192:gustducin; D000089742:Doublecortin-Like Kinases; C485525:Dclk1 protein, mouse; D017346:Protein Serine-Threonine Kinases; D015293:Transducin