title: A microfluidic electrochemical biosensor based on multiwall carbon nanotube/ferrocene for genomic DNA detection of Mycobacterium tuberculosis in clinical isolates.
abstract: Herein we present a microfluidic-multiplexed platform that integrates electrochemical sensors based on carbon nanotubes associated with ferrocene as redox marker (carbon nanotube (CNT)/ferrocene) for direct detection of pathogenic viral DNA from Hepatitis C and genomic DNA from Mycobacterium tuberculosis in clinical isolates. By operating the fluidic device under high flow (150 μl/min), the formation of a very thin depletion layer at the sensor surface (δS = 230 nm) enhances the capture rate up to one DNA strand per second. By comparison, this capture rate is only 0.02 molecule/s in a static regime without flow. This fluidic protocol allows thus enhancing the limit of detection of the electrochemical biosensor from picomolar in bulk solution to femtomolar with a large dynamic range from 0.1 fM to 1 pM. Kinetics analysis also demonstrates an enhancement of the rate constant of electron transfer (kS) of the electrochemical process from 1 s(-1) up to 6 s(-1) thanks to the geometry of the miniaturized fluidic electrochemical cell. This microfluidic device working under high flow allows selective direct detection of a Mycobacterium tuberculosis (H37Rv) rpoB allele from clinical isolate extracted DNA. We envision that a microfluidic approach under high flow associated with a multiwall CNT/ferrocene sensor could find useful applications as the point-of-care for multi-target diagnostics of biomarkers in real samples.
authors: B
authors: B
authors: Zribi
authors: Laboratoire de Photonique et de Nanostructures (LPN), CNRS, Université Paris-Saclay , route de Nozay, F-91460 Marcoussis, France.
authors: E
authors: E
authors: Roy
authors: A
authors: A
authors: Pallandre
authors: Laboratoire de Photonique et de Nanostructures (LPN), CNRS, Université Paris-Saclay , route de Nozay, F-91460 Marcoussis, France.
authors: S
authors: S
authors: Chebil
authors: Laboratoire de Physique des Matériaux, LPM FSS, Faculté des Sciences de Sfax, Université de Sfax , Sfax, Tunisia.
authors: M
authors: M
authors: Koubaa
authors: Université Paris-Saclay , UMR-CNRS 8182, Institue de Chimie Moléculaires et Matériaux d'Orsay (ICMMO), Equipe de Chimie Bioorganique et Bioinorganique (ECBB), Bâtiment 420, 91405, Orsay, France.
authors: N
authors: N
authors: Mejri
authors: UMR-CNRS 9198, Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Saclay , Bat 400, 91405 Orsay, France.
authors: H
authors: H
authors: Magdinier Gomez
authors: UMR-CNRS 9198, Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Saclay , Bat 400, 91405 Orsay, France.
authors: C
authors: C
authors: Sola
authors: Université Paris-Saclay , UMR-CNRS 8182, Institue de Chimie Moléculaires et Matériaux d'Orsay (ICMMO), Equipe de Chimie Bioorganique et Bioinorganique (ECBB), Bâtiment 420, 91405, Orsay, France.
authors: H
authors: H
authors: Korri-Youssoufi
authors: Laboratoire de Photonique et de Nanostructures (LPN), CNRS, Université Paris-Saclay , route de Nozay, F-91460 Marcoussis, France.
authors: A-M
authors: AM
authors: Haghiri-Gosnet