title: A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes.
abstract: Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.
authors: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871, Frederiksberg C, Denmark.
authors: Morten Egevang
authors: ME
authors: Jørgensen
authors: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871, Frederiksberg C, Denmark.
authors: Hussam Hassan
authors: HH
authors: Nour-Eldin
authors: DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871, Frederiksberg C, Denmark. bah@plen.ku.dk.
authors: Barbara Ann
authors: BA
authors: Halkier
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D015870:Gene Expression; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins
keywords: Single oocyte Western blot; Transport protein quantification; Xenopus oocytes