title: Salivary Biomarkers for Detection of Oral Squamous Cell Carcinoma in a Taiwanese Population.
abstract: This study evaluated the discriminatory power of salivary transcriptomic and proteomic biomarkers in distinguishing oral squamous cell carcinoma cases from controls and potentially malignant oral disorders (PMOD).
  
  
  
  A total of 180 samples (60 OSCC patients, 60 controls, and 60 PMOD patients) were used in the study. Seven transcriptomic markers (IL8, IL1β, SAT1, OAZ1, DUSP1, S100P, and H3F3A) were measured using qPCR, and two proteomic markers (IL8 and IL1β) were evaluated by ELISA.
  
  
  
  Among 7 transcriptomic markers, transcript level of DUSP1 was significantly lower in OSCC patients than in controls and PMOD patients. Between the proteomic markers, the protein concentration of IL8 and IL1β was significantly higher in OSCC patients than controls and dysplasia patients. Univariate fractional polynomial (FP) models revealed that salivary IL8 protein (IL8p) has the highest AUC value between OSCC patients and controls (0.74) and between OSCC and PMOD patients (0.72). Applying a 2-marker FP model, salivary IL8p combined with IL1β gave the best AUC value for discrimination between OSCC patients and controls, as well as the IL8p combined with H3F3A mRNA, which gave the best AUC value for discrimination between OSCC and PMOD patients. Multivariate models analysis combining salivary analytes and risk factor exposure related to oral carcinogenesis formed the best combinatory variables for differentiation between OSCC versus PMOL (AUC = 0.80), OSCC versus controls (AUC = 0.87), and PMOD versus controls (AUC = 0.78).
  
  
  
  The combination of transcriptomic and proteomic salivary markers is of great value for oral cancer detection and differentiation from PMOD patients and controls. Clin Cancer Res; 22(13); 3340-7. ©2016 AACR.
authors: Center for Oral/Head & Neck Oncology Research, University of California, Los Angeles, Los Angeles, California. Medical Genomics Laboratory, Centro Internacional de Pesquisa e Ensino - CIPE, AC Camargo Cancer Center, São Paulo, Brazil.
authors: Frederico Omar
authors: FO
authors: Gleber-Netto
authors: Center for Oral/Head & Neck Oncology Research, University of California, Los Angeles, Los Angeles, California.
authors: Maha
authors: M
authors: Yakob
authors: Center for Oral/Head & Neck Oncology Research, University of California, Los Angeles, Los Angeles, California.
authors: Feng
authors: F
authors: Li
authors: Department of Biostatistics, The University of Texas, MD Anderson Cancer Center, Houston, Texas.
authors: Ziding
authors: Z
authors: Feng
authors: Department of Biostatistics, The University of Texas, MD Anderson Cancer Center, Houston, Texas.
authors: Jianliang
authors: J
authors: Dai
authors: Department of Plastic & Reconstructive Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors: Huang-Kai
authors: HK
authors: Kao
authors: Department of Oral and Maxillofacial Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors: Yu-Liang
authors: YL
authors: Chang
authors: Department of Otolaryngology - Head & Neck Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan. College of Medicine, Chang Gung University, Taoyuan, Taiwan. dtww@ucla.edu dr.kpchang@gmail.com.
authors: Kai-Ping
authors: KP
authors: Chang
authors: Center for Oral/Head & Neck Oncology Research, University of California, Los Angeles, Los Angeles, California. dtww@ucla.edu dr.kpchang@gmail.com.
authors: David T W
authors: DT
authors: Wong
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000737:chemistry*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D054638:Dual Specificity Phosphatase 1 / Q000032:analysis*; D055088:Early Detection of Cancer / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006258:Head and Neck Neoplasms / Q000175:diagnosis*; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis*; D016209:Interleukin-8 / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis*; D040901:Proteomics / Q000379:methods; D012307:Risk Factors; D012463:Saliva / Q000737:chemistry*; D000077195:Squamous Cell Carcinoma of Head and Neck; D013624:Taiwan
chemical_list: D014408:Biomarkers, Tumor; C508606:CXCL8 protein, human; C508585:IL1B protein, human; D053583:Interleukin-1beta; D016209:Interleukin-8; C517019:DUSP1 protein, human; D054638:Dual Specificity Phosphatase 1