title: Increased expression of dermatopontin and its implications for testicular dysfunction in mice.
abstract: An array of specific and non‑specific molecules, which are expressed in the testis, have been demonstrated to be responsible for testicular function. Our previous study revealed that dermatopontin (DPT) is expressed in Sertoli cells of the testis, however, its roles in testicular function remains somewhat elusive. In the present study, CdCl2‑ and busulfan‑induced testicular dysfunction models were used to investigate the implications of DPT expression for testicular function. The mRNA and protein expression levels of DPT were detected using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. A negative correlation was observed between testicular damage and the expression of DPT, which suggested that an increase in DPT expression may be a marker for testicular dysfunction. This result was corroborated by the finding that transgenic mice exhibiting Sertoli cell‑specific overexpression of DPT exhibited damage to their testicular morphology. Additionally, DPT overexpression in the testis affected the expression levels of claudin‑11 and zonula occludens‑1, which indicated that DPT may affect testicular function by affecting the integrity of the blood‑testis barrier (BTB). In conclusion, the present study provided evidence to suggest that DPT may be indicative of mouse testicular dysfunction, since increased expression may be associated with damage to the BTB.
authors: Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Jun
authors: J
authors: Cai
authors: Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Weijia
authors: W
authors: Liu
authors: Experimental Research Center, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Jie
authors: J
authors: Hao
authors: Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Maoxin
authors: M
authors: Chen
authors: Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, P.R. China.
authors: Gang
authors: G
authors: Li
mesh_terms: D000818:Animals; D001814:Blood-Testis Barrier / Q000378:metabolism / Q000473:pathology; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism*; D057167:Claudins / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D007248:Infertility, Male / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D057167:Claudins; C492354:Cldn11 protein, mouse; C504453:Dpt protein, mouse; D016326:Extracellular Matrix Proteins; C569043:Tjp1 protein, mouse; D062826:Zonula Occludens-1 Protein