title: HUMAN DNA QUANTIFICATION IN THE STOOLS OF PATIENTS WITH COLORECTAL CANCER.
abstract: BACKGROUND
  Colorectal cancer is one of the main cause of cancer in the world. Colonoscopy is the best screen method, however the compliance is less than 50%. Quantification of human DNA (hDNA) in the feces may be a possible screen non-invasive method that is a consequence of the high proliferation and exfoliation of cancer cells.
  
  
  OBJECTIVE
  To quantify the human DNA in the stools of patients with colorectal cancer or polyps.
  
  
  METHODS
  Fifty patients with CRC, 26 polyps and 53 with normal colonoscopy were included. Total and human DNA were analyzed from the frozen stools.
  
  
  RESULTS
  An increased concentration of hDNA in the stools was observed in colorectal cancer patients compared to controls and polyps. Tumors localized in the left side of the colon had higher concentrations of hDNA. There were no difference between polyps and controls. A cut off of 0.87 ng/mL of human DNA was determined for colorectal cancer patients by the ROC curve, with a sensitivity of 66% and a specificity of 86.8%. For polyps the cut off was 0.41, the sensitivity was 41% and the specificity 77.4%.
  
  
  CONCLUSIONS
  A higher concentration of hDNA had been found in colorectal cancer patients The quantification of hDNA from the stools can be a trial method for the diagnosis of colorectal cancer.
authors: Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: Yolanda
authors: Y
authors: Teixeira
authors: Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: Jacqueline Miranda
authors: JM
authors: Lima
authors: Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: Maria Luiza Almeida Prado Oliveira
authors: ML
authors: Souza
authors: Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: Pedro
authors: P
authors: Aguiar
authors: Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: Tiago Donizetti
authors: TD
authors: Silva
authors: Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: Nora Manoukian
authors: NM
authors: Forones
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D016022:Case-Control Studies; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012372:ROC Curve; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm