title: Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography.
abstract: This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. The ultrasound-assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high-speed counter-current chromatography without any pretreatment using n-hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by (1) H NMR spectroscopy.
authors: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|University of the Chinese Academy of Sciences, Beijing, P.R. China.
authors: Deng-lang
authors: DL
authors: Zou
authors: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
authors: Tao
authors: T
authors: Chen
authors: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|University of the Chinese Academy of Sciences, Beijing, P.R. China.
authors: Hong-mei
authors: HM
authors: Li
authors: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
authors: Chen
authors: C
authors: Chen
authors: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
authors: Jing-yang
authors: JY
authors: Zhao
authors: Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.|Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, P. R. China.
authors: Yu-lin
authors: YL
authors: Li
mesh_terms: D003377:Countercurrent Distribution; D008970:Molecular Weight; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D029766:Terminalia / Q000737:chemistry*; D014465:Ultrasonics
chemical_list: D010636:Phenols; D010936:Plant Extracts
keywords: Crude extracts; High-speed counter-current chromatography; Phenolic compounds; Response surface methodology; Terminalia chebula Retz