title: Histological and transcriptome analyses of testes from Duroc and Meishan boars.
abstract: Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and transcriptome analyses. Haematoxylin-eosin staining was conducted to observe histological structure of the testes in Duroc and Meishan boars at different ages. Although spermatogenesis occurred prior to 75 days in Meishan boars, the number of spermatogonia and Sertoli cells in Meishan boars were less than in Duroc boars at adulthood. The diameters of the seminiferous tubules of the testes differed significantly during the initiation of development of the seminiferous tubules between the two breeds. We obtained differentially expressed functional genes and analysed seven pathways involved in male sexual maturity and spermatogenesis using RNA-seq. We also detected four main alternative splicing events and many single nucleotide polymorphisms from testes. Eight functionally important genes were validated by qPCR, and Neurotrophin 3 was subjected to quantification and cellular localization analysis. Our study provides the first transcriptome evidence for the differences in sexual function development between Meishan and Duroc boars.
authors: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors: Haisheng
authors: H
authors: Ding
authors: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors: Yan
authors: Y
authors: Luo
authors: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors: Min
authors: M
authors: Liu
authors: Animal Husbandry and Veterinary Bureau of Hubei Province, Wuhan 430064, China.
authors: Jingshu
authors: J
authors: Huang
authors: Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, and Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors: Dequan
authors: D
authors: Xu
mesh_terms: D000818:Animals; D001947:Breeding; D004801:Eosine Yellowish-(YS); D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006416:Hematoxylin; D008297:Male; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism; D009929:Organ Size; D020641:Polymorphism, Single Nucleotide; D012671:Seminiferous Tubules / Q000378:metabolism* / Q000648:ultrastructure; D017423:Sequence Analysis, RNA; D012708:Sertoli Cells / Q000378:metabolism* / Q000648:ultrastructure; D012741:Sexual Maturation / Q000235:genetics*; D013045:Species Specificity; D013076:Sperm Count; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure; D013552:Swine; D059467:Transcriptome
chemical_list: D020933:Neurotrophin 3; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin