title: The regulation mechanism of apoptosis by visfatin in the mesenteric lymph nodes of LPS-treated rats.
abstract: Visfatin is an adipocytokine displaying multiple functional properties, which plays a role in the regulation of cell apoptosis and inflammation by an as yet unidentified mechanism. The aim of the present study was to determine if visfatin is involved in apoptosis pathway induced by LPS in rat Mesenteric lymph nodes (MLNs). Experimental rats were divided into four groups and MLNs samples were collected from each group. The morphological changes of the MLNs were examined by histological imaging. CD68 and ENPP1 were detected with immunohistochemistry and Western Blot. Apoptosis was evaluated with TUNEL and Flow Cytometry, the mRNA levels of the apoptosis-related genes were detected by qRT-PCR, and the protein levels of the apoptotic-related factors were detected by western blot. The main results showed that visfatin could significantly increase the macrophages in MLNs and prevent cell apoptosis from LPS-induced mesenteric lymph nodes, activate apoptotic signaling pathways and regulate the mRNA levels of the apoptosis-related genes. Visfatin had a pro-apoptotic effect on normal MLNs, whereas it exerted an anti-apoptotic effect during LPS-induced cell apoptosis in rat MLNs. In short, visfatin plays a dual role in the apoptosis in rat MLNs, which is mediated by both the mitochondrial apoptotic pathway and the death-receptor apoptotic pathway.
authors: College of veterinary medicine, Huazhong Agricultural University, Wuhan, China.
authors: Ke
authors: K
authors: Xiao
authors: College of Animal Science, Huazhong Agricultural University, Wuhan, China.
authors: Ying
authors: Y
authors: Zhou
authors: College of Animal Science, Huazhong Agricultural University, Wuhan, China.
authors: Huai-Rui
authors: HR
authors: Yuan
authors: College of Animal Science, Huazhong Agricultural University, Wuhan, China.
authors: Lu
authors: L
authors: Cui
authors: College of Animal Science, Huazhong Agricultural University, Wuhan, China.
authors: Zia Ur
authors: ZU
authors: Rehman
authors: College of veterinary medicine, Huazhong Agricultural University, Wuhan, China.
authors: Abdur Rahman
authors: AR
authors: Ansari
authors: College of veterinary medicine, Huazhong Agricultural University, Wuhan, China.
authors: Zhi
authors: Z
authors: Yang
authors: College of veterinary medicine, Huazhong Agricultural University, Wuhan, China.
authors: Ke-Mei
authors: KM
authors: Peng
authors: College of veterinary medicine, Huazhong Agricultural University, Wuhan, China. songh2007@mail.hzau.edu.cn and xke1995@126.com.
authors: Hui
authors: H
authors: Song
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D015153:Blotting, Western; D005434:Flow Cytometry; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008070:Lipopolysaccharides / Q000633:toxicity; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology*; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D008070:Lipopolysaccharides; D054409:Nicotinamide Phosphoribosyltransferase