title: Establishment of a patient-derived intrahepatic cholangiocarcinoma xenograft model with KRAS mutation.
abstract: BACKGROUND
  Intrahepatic cholangiocarcinoma (ICC) is an aggressive, highly lethal tumors and lacks of effective chemo and targeted therapies. Cell lines and animal models, even partially reflecting tumor characteristics, have limits to study ICC biology and drug response. In this work, we created and characterized a novel ICC patient-derived xenograft (PDX) model of Italian origin.
  
  
  METHODS
  Seventeen primary ICC tumors derived from Italian patients were implanted into NOD (Non-Obese Diabetic)/Shi-SCID (severe combined immunodeficient) mice. To verify if the original tumor characteristics were maintained in PDX, immunohistochemical (cytokeratin 7, 17, 19, and epithelial membrane antigen) molecular (gene and microRNA expression profiling) and genetic analyses (comparative genomic hybridization array, and mutational analysis of the kinase domain of EGFR coding sequence, from exons 18 to 21, exons 2 to 4 of K-RAS, exons 2 to 4 of N-RAS, exons 9 and 20 of PI3KCA, and exon 15 of B-RAF) were performed after tumor stabilization.
  
  
  RESULTS
  One out of 17 (5.8%) tumors successfully engrafted in mice. A high molecular and genetic concordance between primary tumor (PR) and PDX was confirmed by the evaluation of biliary epithelial markers, tissue architecture, genetic aberrations (including K-RAS G12D mutation), and transcriptomic and microRNA profiles.
  
  
  CONCLUSIONS
  For the first time, we established a new ICC PDX model which reflects the histology and genetic characteristics of the primary tumor; this model could represent a valuable tool to understand the tumor biology and the progression of ICC as well as to develop novel therapies for ICC patients.
authors: Fondazione del Piemonte per l'Oncologia (FPO), Candiolo Cancer Institute-IRCCS, Candiolo, Italy. giuliana.cavalloni@ircc.it.
authors: Giuliana
authors: G
authors: Cavalloni
authors: Fondazione del Piemonte per l'Oncologia (FPO), Candiolo Cancer Institute-IRCCS, Candiolo, Italy. caterina.peraldoneia@ircc.it.
authors: Caterina
authors: C
authors: Peraldo-Neia
authors: Unit of Molecular Pharmacology, Candiolo Cancer Institute-IRCCS, University of Turin Medical School, Candiolo, Italy. francesco.sassi@ircc.it.
authors: Francesco
authors: F
authors: Sassi
authors: Cancer Genomics Laboratory, Fondazione Edo ed Elvo Tempia Valenta, Biella, Italy. giovanna.chiorino@gmail.com.
authors: Giovanna
authors: G
authors: Chiorino
authors: Fondazione del Piemonte per l'Oncologia (FPO), Unit of Pathology, Candiolo Cancer Institute-IRCCS, Candiolo, Italy. ivana.sarotto@ircc.it.
authors: Ivana
authors: I
authors: Sarotto
authors: Fondazione del Piemonte per l'Oncologia (FPO), Candiolo Cancer Institute-IRCCS, Candiolo, Italy. massimo.aglietta@ircc.it.|Oncology Department, Candiolo Cancer Institute-IRCCS, University of Turin Medical School, Candiolo, Italy. massimo.aglietta@ircc.it.
authors: Massimo
authors: M
authors: Aglietta
authors: Fondazione del Piemonte per l'Oncologia (FPO), Candiolo Cancer Institute-IRCCS, Candiolo, Italy. francesco.leone@ircc.it.|Oncology Department, Candiolo Cancer Institute-IRCCS, University of Turin Medical School, Candiolo, Italy. francesco.leone@ircc.it.
authors: Francesco
authors: F
authors: Leone
mesh_terms: D000818:Animals; D018281:Cholangiocarcinoma / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: C117307:KRAS protein, human; D035683:MicroRNAs; D016283:Proto-Oncogene Proteins p21(ras)