title: Clathrin binding by the adaptor Ent5 promotes late stages of clathrin coat maturation.
abstract: Clathrin is a ubiquitous protein that mediates membrane traffic at many locations. To function, clathrin requires clathrin adaptors that link it to transmembrane protein cargo. In addition to this cargo selection function, many adaptors also play mechanistic roles in the formation of the transport carrier. However, the full spectrum of these mechanistic roles is poorly understood. Here we report that Ent5, an endosomal clathrin adaptor in Saccharomyces cerevisiae, regulates the behavior of clathrin coats after the recruitment of clathrin. We show that loss of Ent5 disrupts clathrin-dependent traffic and prolongs the lifespan of endosomal structures that contain clathrin and other adaptors, suggesting a defect in coat maturation at a late stage. We find that the direct binding of Ent5 with clathrin is required for its role in coat behavior and cargo traffic. Surprisingly, the interaction of Ent5 with other adaptors is dispensable for coat behavior but not cargo traffic. These findings support a model in which Ent5 clathrin binding performs a mechanistic role in coat maturation, whereas Ent5 adaptor binding promotes cargo incorporation.
authors: Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.
authors: Chao-Wei
authors: CW
authors: Hung
authors: Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 mcduncan@med.umich.edu.
authors: Mara C
authors: MC
authors: Duncan
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D002966:Clathrin / Q000378:metabolism*; D022163:Clathrin-Coated Vesicles / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D002966:Clathrin; C495588:Ent5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins