title: Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii.
abstract: A. baumannii infections are becoming more and more serious health issues with rapid emerging of multidrug and extremely drug resistant strains, and therefore, there is an urgent need for the development of nonantibiotic-based intervention strategies. This study aimed at identifying whether an outer membrane protein with molecular weight of about 22 kDa (Omp22) holds the potentials to be an efficient vaccine candidate and combat A. baumannii infection. Omp22 which has a molecule length of 217 amino acids kept more than 95% conservation in totally 851 reported A. baumannii strains. Recombinant Omp22 efficiently elicited high titers of specific IgG in mice. Both active and passive immunizations of Omp22 increased the survival rates of mice, suppressed the bacterial burdens in the organs and peripheral blood, and reduced the levels of serum inflammatory cytokines and chemokines. Opsonophagocytosis assays showed in vitro that Omp22 antiserum had highly efficient bactericidal activities on clonally distinct clinical A. baumannii isolates, which were partly complements-dependent and opsonophagocytic killing effects. Additionally, administration with as high as 500 μg of Omp22 didn't cause obvious pathological changes in mice. In conclusion, Omp22 is a novel conserved and probably safe antigen for developing effective vaccines or antisera to control A. baumannii infections.
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Weiwei
authors: W
authors: Huang
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Yufeng
authors: Y
authors: Yao
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Shijie
authors: S
authors: Wang
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Ye
authors: Y
authors: Xia
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Xu
authors: X
authors: Yang
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Qiong
authors: Q
authors: Long
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Wenjia
authors: W
authors: Sun
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Cunbao
authors: C
authors: Liu
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Yang
authors: Y
authors: Li
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Xiaojie
authors: X
authors: Chu
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Hongmei
authors: H
authors: Bai
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Yueting
authors: Y
authors: Yao
authors: Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences &Peking Union Medical College; Kunming, China 650118.|Yunnan Key Laboratory of Vaccine Research &Development on Severe Infectious Diseases; Kunming, China 650118.|Yunnan Engineering Research Center of Vaccine Research and Development on Severe Infectious Diseases, Kunming, China 650118.
authors: Yanbing
authors: Y
authors: Ma
mesh_terms: D000151:Acinetobacter Infections / Q000517:prevention & control*; D040981:Acinetobacter baumannii / Q000276:immunology*; D000825:Animal Structures / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D058491:Bacterial Load; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D001769:Blood / Q000382:microbiology; D003165:Complement System Proteins; D017124:Conserved Sequence; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000097:blood; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D044967:Serum / Q000737:chemistry; D016019:Survival Analysis; D014611:Vaccination; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D003165:Complement System Proteins