title: A betabaculovirus encoding a gp64 homolog.
abstract: BACKGROUND
  A betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil.
  
  
  RESULTS
  The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7% encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister group to a cluster formed by ChocGV, ErelGV, PiraGV isolates, ClanGV, CaLGV, CpGV, CrleGV, AdorGV, PhopGV and EpapGV. Surprisingly, we found in the DisaGV genome a G protein-coupled receptor related to lepidopteran and other insect virus genes and a gp64 homolog, which is likely a product of horizontal gene transfer from Group 1 alphabaculoviruses.
  
  
  CONCLUSIONS
  DisaGV represents a distinct lineage of the genus Betabaculovirus. It is closely related to the CpGV-related group and presents the smallest genome in size so far. Remarkably, we found a homolog of gp64, which was reported solely in group 1 alphabaculovirus genomes so far.
authors: Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil. daniel_ardisson@yahoo.com.br.
authors: Daniel M P
authors: DM
authors: Ardisson-Araújo
authors: Programa de Pós-graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil. bruna.btp13@gmail.com.
authors: Bruna T
authors: BT
authors: Pereira
authors: Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil. flucasmelo@gmail.com.
authors: Fernando L
authors: FL
authors: Melo
authors: Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil. bergmann@unb.br.
authors: Bergmann M
authors: BM
authors: Ribeiro
authors: Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil. snbao@unb.br.
authors: Sônia N
authors: SN
authors: Báo
authors: Laboratório de Evolução Molecular e Bioinformática (LEMB-ICB), Universidade de São Paulo, São Paulo, SP, Brazil. pzanotto@usp.br.
authors: Paolo M
authors: PM
authors: de A Zanotto
authors: Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Soja, Londrina, Paraná PR, Brazil.
authors: Flávio
authors: F
authors: Moscardi
authors: NAP/MEPA, Departamento de Fitopatologia e Nematologia, ESALQ, Universidade de São Paulo, Piracicaba, SP, Brazil. ewkitaji@usp.br.
authors: Elliot W
authors: EW
authors: Kitajima
authors: Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Soja, Londrina, Paraná PR, Brazil. daniel.sosa-gomez@embrapa.br.
authors: Daniel R
authors: DR
authors: Sosa-Gomez
authors: Laboratório de Biologia Molecular e Virologia, Centro de Ciências Biológicas e da Saúde (CCBS), Universidade Presbiteriana Mackenzie, São Paulo, SP, Brazil. joseluiz.wolff@mackenzie.br.
authors: José L C
authors: JL
authors: Wolff
mesh_terms: D016367:Baculoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D001482:Base Composition; D001483:Base Sequence; D001938:Brazil; D023061:Gene Order; D016679:Genome, Viral; D023281:Genomics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D031786:Saccharum / Q000821:virology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; D014764:Viral Proteins