title: Identification and Determination of the Polyhydroxylated Alkaloids Compounds with α-Glucosidase Inhibitor Activity in Mulberry Leaves of Different Origins.
abstract: Mulberry leaves have commonly been utilized in China as a herbal medicine for the treatment of diabetes for thousands of years. To evaluate the quality, an ultra-high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) method was developed for identification of polyhydroxylated alkaloids with α-glucosidase inhibitor activity in mulberry leaf. As a result, five alkaloid compounds were identiﬁed or tentatively characterized. Among them, the compound 1-deoxynojirimycin (DNJ) was selected as the most typical and active chemical marker and quantiﬁed using an improved high performance liquid chromatography (HPLC) normal phase coupled with evaporative light scattering detector (ELSD) method. The developed method was fully validated in terms of linearity, sensitivity, precision and repeatability, as well as recovery, and subsequently applied to evaluate twenty-nine batches of mulberry leaves from different collections. From the analytical data it was discovered that the average content of DNJ is 1.53 mg/g, while the total contents of DNJ in the 29 mulberry leaf sample ranged from 0.20 to 3.88 mg/g, which suggested remarkable differences, although it reached the highest levels in early August. These data may provide an important reference for the quality of mulberry leaves used as herbal medicine for the treatment of diabetes or as a material to obtain the DNJ of α-glucosidase inhibitor or as a functional food.
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China. nianningtao@163.com.
authors: Tao
authors: T
authors: Ji
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China. justleejun@sina.com.
authors: Jun
authors: J
authors: Li
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China. shulansu@njucm.edu.cn.
authors: Shu-Lan
authors: SL
authors: Su
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China. 04040416@163.com.
authors: Zhen-Hua
authors: ZH
authors: Zhu
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China. gsh916@163.com.
authors: Sheng
authors: S
authors: Guo
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China. qiandwnj@126.com.
authors: Da-Wei
authors: DW
authors: Qian
authors: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China. duanja@163.com.
authors: Jin-Ao
authors: JA
authors: Duan
mesh_terms: D017485:1-Deoxynojirimycin / Q000737:chemistry / Q000302:isolation & purification*; D000470:Alkaloids / Q000737:chemistry* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000470:Alkaloids; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D017485:1-Deoxynojirimycin
keywords: 1-deoxynojirimycin; HPLC-ELSD; cluster analysis; mulberry leaves; quality control