title: Effect of SIRT1 Gene on Epithelial-Mesenchymal Transition of Human Prostate Cancer PC-3 Cells.
abstract: BACKGROUND The epithelial-mesenchymal transition (EMT) has been shown to be involved in the process of invasion and metastasis of prostate cancer. SIRT1 is the mammalian homologue of the silent information regulator 2 (Sir2) gene, and is abnormally expressed in prostate cancer cells. Therefore, it is hypothesized that SIRT1 mediates the invasion/metastatic ability of prostate cancer via EMT regulation. This study thus investigated the effect of SIRT1 gene on the invasion and migration of prostate cancer cell line PC-3 via the small interference RNA (siRNA) against SIRT1. MATERIAL AND METHODS SiRNA construct was transfected into PC-3 cells, which were tested for the cell migration and invasion ability by scratch assay and Transwell migration assay, respectively. Expression levels of vimentin, E-cadherin, and N-cadherin were further quantified by Western blotting and RT-PCR. RESULTS Both mRNA and protein levels of SIRT1 were depressed after siRNA transfection, along with weakened migration and invasion ability of PC-3 cells. Elevated E-cadherin and suppressed N-cadherin and vimentin were observed in those transfected cells. CONCLUSIONS The silencing of SIRT1 gene in PC-3 cells can suppress the movement, migration, and invasion functions of prostate cancer cells, possibly via the down-regulation of mesenchymal markers vimentin and N-cadherin accompanied with up-regulation of epithelial marker N-cadherin, thus reversing the EMT process.
authors: Department of Blood Transfusion, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Ying
authors: Y
authors: Cui
authors: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Jiang
authors: J
authors: Li
authors: Department of Hepatobiliary Surgery, General Hospital of Shenyang Military Command, Shenyang, Liaoning, China (mainland).
authors: Fei
authors: F
authors: Zheng
authors: Department of Medical Laboratory and Research Center, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Yongri
authors: Y
authors: Ouyang
authors: Department of Medical Laboratory and Research Center, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Xi
authors: X
authors: Chen
authors: Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Lei
authors: L
authors: Zhang
authors: Department of Blood Transfusion, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Yang
authors: Y
authors: Chen
authors: Department of Blood Transfusion, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Lin
authors: L
authors: Wang
authors: Department of Blood Transfusion, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Shijie
authors: S
authors: Mu
authors: Department of Medical Laboratory and Research Center, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shanxi, China (mainland).
authors: Huizhong
authors: H
authors: Zhang
mesh_terms: D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; D034741:RNA, Small Interfering; D014746:Vimentin; D043366:Cellulose 1,4-beta-Cellobiosidase; C447939:SIRT1 protein, human; D056564:Sirtuin 1