title: Long non-coding RNA FEZF1-AS1 facilitates cell proliferation and migration in colorectal carcinoma.
abstract: Long non-coding RNAs (lncRNA) have been shown to play important roles in the development and progression of cancer. Here, we discovered a novel long noncoding RNA (lncRNA) FEZF1 antisense RNA1 (FEZF1-AS1) is markedly upregulated in human primary colorectal carcinoma (CRC) and associated with CRC metastasis and poor prognosis. Moreover, the downregulation of FEZF1-AS1 expression significantly inhibited the CRC cells proliferation, migration and invasiveness, suppressed S-phase entry in vitro, and repressed tumor growth and metastasis in vivo. In contrast, overexpression of FEZF1-AS1 could promote the aggressive behaviors of CRC cells. We further discovered that the downregulation of FEZF1-AS1 reduced its sense-cognate gene FEZF1 mRNA and protein expression in CRC cells. There was a positive correlation between FEZF1-AS1 and FEZF1 expression in CRC. Moreover, FEZF1 knockdown also significantly suppressed CRC cell proliferation, migration, and invasion. Our findings indicate that the dysregulation of FEZF1-AS1 participates in colorectal tumorigenesis and progression, which might be achieved, at least in part, through FEZF1 induction.
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors: Na
authors: N
authors: Chen
authors: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Dan
authors: D
authors: Guo
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors: Qiong
authors: Q
authors: Xu
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors: Minhui
authors: M
authors: Yang
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Dan
authors: D
authors: Wang
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors: Man
authors: M
authors: Peng
authors: Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors: Yanqing
authors: Y
authors: Ding
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors: Shuang
authors: S
authors: Wang
authors: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
authors: Jun
authors: J
authors: Zhou
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D057809:HEK293 Cells; D019073:HT29 Cells; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D011379:Prognosis; D016372:RNA, Antisense / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D022661:RNA, Untranslated / Q000096:biosynthesis / Q000235:genetics*; D012097:Repressor Proteins; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C000595492:FEZF1 protein, human; D016372:RNA, Antisense; D012333:RNA, Messenger; D022661:RNA, Untranslated; D012097:Repressor Proteins; D014157:Transcription Factors
keywords: FEZF1; FEZF1-AS1; colorectal cancer; long non-coding RNA