title: Investigation of Viral and Host Chromatin by ChIP-PCR or ChIP-Seq Analysis.
abstract: Complex regulation of viral transcription patterns and DNA replication levels is a feature of many DNA viruses. This is especially true for those viruses which establish latent or persistent infections (e.g., herpesviruses, papillomaviruses, polyomaviruses, or adenovirus), as long-term persistence often requires adaptation of gene expression programs and/or replication levels to the cellular milieu. A key factor in the control of such processes is the establishment of a specific chromatin state on promoters or replication origins, which in turn will determine whether or not the underlying DNA is accessible for other factors that mediate downstream processes. Chromatin immunoprecipitation (ChIP) is a powerful technique to investigate viral chromatin, in particular to study binding patterns of modified histones, transcription factors or other DNA-/chromatin-binding proteins that regulate the viral lifecycle. Here, we provide protocols that are suitable for performing ChIP-PCR and ChIP-Seq studies on chromatin of large and small viral genomes.
authors: Heinrich-Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
authors: Thomas
authors: T
authors: Günther
authors: Heinrich-Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.|Institute for Medical Microbiology, Virology and Hygiene; University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors: Juliane M
authors: JM
authors: Theiss
authors: Institute for Medical Microbiology, Virology and Hygiene; University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors: Nicole
authors: N
authors: Fischer
authors: Heinrich-Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
authors: Adam
authors: A
authors: Grundhoff
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004279:DNA, Viral; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D002843:Chromatin; D004279:DNA, Viral
keywords: ChIP; ChIP-PCR; ChIP-seq; chromatin; next-generation sequencing; viral chromatin