title: Correlation Between DNA Methylation of TRPA1 and Chronic Pain States in Human Whole Blood Cells.
abstract: Neuro-immune interactions with functional changes in the peripheral blood cells including changes in the transient receptor potential ankyrin 1 (TRPA1) appear to play a pivotal role in the development of chronic pain in humans. The aim of this study was to examine the association between TRPA1 DNA methylation in whole blood cells and the pain states in chronic pain patients.
  
  
  
  After collecting blood samples from 12 chronic pain patients, the authors measured DNA methylation levels in whole blood cells. Significant associations between the patient's demographic data and the chronic pain states were determined by a multiple linear regression analysis that used age, body mass index, pain duration, depression, anxiety, cognitive impairment, activities of daily living, neuropathic pain, and pain states as the dependent variables, and the TRPA1 DNA methylation levels as the independent variables.
  
  
  
  Multiple regression analysis revealed a significant correlation between increases of the methylation levels of the CpG island in the TRPA1 gene and increases in the number of neuropathic pain symptoms, which were evaluated using the Douleur Neuropathique 4 (DN4) questionnaire. Decreases in the TRPA1 mRNA expression were also significantly related to increases in the DN4 score. The presence of a burning sensation, which is one of pain symptoms in the DN4 questionnaire, was significantly correlated with the increase in DNA methylation level of TRPA1.
  
  
  
  TRPA1 DNA methylation levels in whole blood cells appear to be associated with pain symptoms in chronic pain patients.
authors: *Department of Anesthesiology and Pain Medicine.
authors: Norihiko
authors: N
authors: Sukenaga
authors: *Department of Anesthesiology and Pain Medicine.
authors: Yasuko
authors: Y
authors: Ikeda-Miyagawa
authors: *Department of Anesthesiology and Pain Medicine.
authors: Daisuke
authors: D
authors: Tanada
authors: Pain Clinic.
authors: Takashi
authors: T
authors: Tunetoh
authors: Pain Clinic.
authors: Susumu
authors: S
authors: Nakano
authors: Pain Clinic.
authors: Takae
authors: T
authors: Inui
authors: Pain Clinic.
authors: Kazumi
authors: K
authors: Satoh
authors: *Department of Anesthesiology and Pain Medicine.|Department of Anatomy and Neuroscience, Hyogo College of Medicine, Hyogo, Japan.
authors: Hiroai
authors: H
authors: Okutani
authors: Department of Anatomy and Neuroscience, Hyogo College of Medicine, Hyogo, Japan.
authors: Koichi
authors: K
authors: Noguchi
authors: *Department of Anesthesiology and Pain Medicine mhirose@hyo-med.ac.jp.|Pain Clinic.
authors: Munetaka
authors: M
authors: Hirose
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D001773:Blood Cells / Q000378:metabolism*; D015220:Calcium Channels / Q000097:blood*; D059350:Chronic Pain / Q000097:blood* / Q000175:diagnosis; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000097:blood*; D010147:Pain Measurement / Q000379:methods*; D000074025:TRPA1 Cation Channel; D050051:Transient Receptor Potential Channels / Q000097:blood*
chemical_list: D015415:Biomarkers; D015220:Calcium Channels; D009419:Nerve Tissue Proteins; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050051:Transient Receptor Potential Channels
keywords: DN4; Epigenetics; Neuropathic pain; TRPA1