title: Oceanobacillus halophilussp. nov., a novel moderately halophilic bacterium from a hypersaline lake.
abstract: A moderately halophilic bacterium was isolated from a brine sample of a hypersaline lake, Aran-Bidgol, in Iran. The strain, designated J8BT, was Gram-stain-positive, endospore-forming, rod-shaped, strictly aerobic, motile and produced cream colonies. Strain J8BTgrew in NaCl at between 3.0-15.0 % (w/v) (optimally at 7.5 % NaCl, w/v), between pH 6.5-9.0 (optimally at pH 8.0) and between 20-45 °C (optimally at 35 °C). Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain J8BTis a member of the genusOceanobacillusand most closely related toOceanobacillus profundusCL-MP28T,Oceanobacillus polygoniSA9TandOceanobacillus oncorhynchiR-2T(96.9 %, 96.3 % and 96.2 % similarities, respectively). The level of DNA-DNA relatedness between the novel isolate andO. profundusIBRC-M 10567Twas 10 %. The major cellular fatty acids of the isolate were anteiso-C15 : 0, iso-C15 : 0and anteiso-C17 : 0. The polar lipid pattern of strain J8BTconsisted of phosphatidylglycerol, diphosphatidylglycerol, five phospholipids, two aminolipids and two glycoaminolipids. It contained MK-7 as the predominant menaquinone andmeso-diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 39.2 mol%. Phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data suggest that this strain represents a novel species of the genusOceanobacillus, for which the nameOceanobacillus halophilussp. nov. is proposed. The type strain is strain J8BT( = IBRC-M 10444T= DSM 23996T).
authors: Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran,Tehran,Iran.
authors: Mohammad Ali
authors: MA
authors: Amoozegar
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC),ACECR, Tehran,Iran.
authors: Maryam
authors: M
authors: Bagheri
authors: Department of Biology, Faculty of Science, Ferdowsi University of Mashhad,Mashhad,Iran.
authors: Ali
authors: A
authors: Makhdoumi
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC),ACECR, Tehran,Iran.
authors: Mahdi Moshtaghi
authors: MM
authors: Nikou
authors: Microorganisms Bank, Iranian Biological Resource Centre (IBRC),ACECR, Tehran,Iran.
authors: Seyed Abolhassan Shahzadeh
authors: SAS
authors: Fazeli
authors: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,Inhoffenstraße 7B, 38124 Braunschweig,Germany.
authors: Peter
authors: P
authors: Schumann
authors: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures,Inhoffenstraße 7B, 38124 Braunschweig,Germany.
authors: Cathrin
authors: C
authors: Spröer
authors: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla,41012 Sevilla,Spain.
authors: Cristina
authors: C
authors: Sánchez-Porro
authors: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla,41012 Sevilla,Spain.
authors: Antonio
authors: A
authors: Ventosa