title: Mini-P-gp and P-gp Co-Expression in Brown Trout Erythrocytes: A Prospective Blood Biomarker of Aquatic Pollution.
abstract: In aquatic organisms, such as fish, blood is continually exposed to aquatic contaminants. Multidrug Resistance (MDR) proteins are ubiquitous detoxification membrane pumps, which recognize various xenobiotics. Moreover, their expression is induced by a large class of drugs and pollutants. We have highlighted the co-expression of a mini P-gp of 75 kDa and a P-gp of 140 kDa in the primary culture of brown trout erythrocytes and in the erythrocytes of wild brown trout collected from three rivers in the Auvergne region of France. In vitro experiments showed that benzo[a]pyrene, a highly toxic pollutant model, induced the co-expression of mini-P-gp and P-gp in trout erythrocytes in a dose-dependent manner and relay type response. Similarly, in the erythrocytes of wild brown trout collected from rivers contaminated by a mixture of PAH and other multi-residues of pesticides, mini-P-gp and P-gp were able to modulate their expression, according to the nature of the pollutants. The differential and complementary responses of mini-P-gp and P-gp in trout erythrocytes suggest the existence in blood cells of a real protective network against xenobiotics/drugs. This property could be exploited to develop a blood biomarker of river pollution.
authors: ERTICa-EA 4677, Département Génie Biologique, Institut Universitaire de Technologie, Clermont Université-Université d'Auvergne, Ensemble universitaire des Cézeaux, B.P. 86-63172 Aubiere, France. emeline.valton@gmail.com.|LMGE-UMR CNRS 6023, Clermont Université-Université Blaise Pascal, B.P. 80026-63171 Aubiere, France. emeline.valton@gmail.com.|Fédération pour la Pêche et la Protection du Milieu Aquatique du Puy de Dôme (F.P.P.M.A. 63), 14 Allée des Eaux et Forêts, Site de Marmilhat Sud, 63370 Lempdes, France. emeline.valton@gmail.com.
authors: Emeline
authors: E
authors: Valton
authors: LMGE-UMR CNRS 6023, Clermont Université-Université Blaise Pascal, B.P. 80026-63171 Aubiere, France. christian.amblard@univ-bpclermont.fr.
authors: Christian
authors: C
authors: Amblard
authors: Fédération pour la Pêche et la Protection du Milieu Aquatique du Puy de Dôme (F.P.P.M.A. 63), 14 Allée des Eaux et Forêts, Site de Marmilhat Sud, 63370 Lempdes, France. f.desmolles@peche63.com.
authors: François
authors: F
authors: Desmolles
authors: Rovaltain Research Company, 1 avenue de la gare, B.P. 10313, 26958 Valence Cedex 09, France. bcombourieu@rovaltainresearch.com.
authors: Bruno
authors: B
authors: Combourieu
authors: ERTICa-EA 4677, Centre Jean Perrin, Clermont Université-Université d'Auvergne, 58 rue Montalembert, B.P. 392-63011 Clermont-Ferrand, France. frederique.penault-llorca@cjp.fr.
authors: Frédérique
authors: F
authors: Penault-Llorca
authors: ERTICa-EA 4677, Département Génie Biologique, Institut Universitaire de Technologie, Clermont Université-Université d'Auvergne, Ensemble universitaire des Cézeaux, B.P. 86-63172 Aubiere, France. mahchid.bamdad@udamail.fr.
authors: Mahchid
authors: M
authors: Bamdad
keywords: P-gp; blood biomarker; brown trout; erythrocytes; mini-P-gp; river pollution