title: Cultivation of Neochloris oleoabundans in bubble column photobioreactor with or without localized deoxygenation.
abstract: This study evaluated long-term non-sterile cultivation of freshwater green alga Neochloris oleoabundans in a 15-liter bubble column photobioreactor (BCPBR) and the effects of a membrane-based localized oxygen remover (LOR) on deoxygenation, cell growth, and lipid production of N. oleoabundans. Batch and continuous cultivations were carried out under non-sterile conditions for 53 days with no detectable protozoa or other biological contaminants, indicating successful long-term contamination-free cultivation. The results show that the BCPBR equipped with LOR (BCPBR-LOR) has enhanced deoxygenation efficiency and were able to maintain dissolved oxygen at a level of around 120% air saturation, which was 32% lower than that of the conventional BCPBR, which had no LOR. While similar biomass concentration and productivity were obtained in both systems, significantly higher lipid cell content and lipid productivity of microalgae were obtained in the latter, which was attributed to the low dO2 in culture due to enhanced deoxygenation efficiency of BCPBR-LOR.
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors: Licheng
authors: L
authors: Peng
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors: Zisheng
authors: Z
authors: Zhang
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
authors: Peiyao
authors: P
authors: Cheng
authors: Department of Chemical Engineering, Nanjing Tech University, Nanjing, China.
authors: Zhaohui
authors: Z
authors: Wang
authors: Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada. Electronic address: Christopher.Lan@uottawa.ca.
authors: Christopher Q
authors: CQ
authors: Lan
mesh_terms: D018533:Biomass; D000460:Chlorophyta / Q000254:growth & development* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism; D010100:Oxygen / Q000302:isolation & purification*; D058433:Photobioreactors / Q000382:microbiology*; D010860:Pigments, Biological / Q000378:metabolism; D012995:Solubility; D013696:Temperature; D013997:Time Factors
chemical_list: D008055:Lipids; D010860:Pigments, Biological; D010100:Oxygen
keywords: Bubble column photobioreactor; Continuous; Localized oxygen remover; Neochloris oleoabundans; Non-sterile cultivation