title: A novel dermal matrix generated from burned skin as a promising substitute for deep-degree burns therapy.
abstract: The extensive skin defects induced by severe burns are dangerous and can be fatal. Currently, the most common therapy is tangential excision to remove the necrotic or denatured areas of skin, followed by skin grafting. Xenogeneic dermal substitutes, such as porcine acellular dermal matrix (ADM), are typically used to cover the burn wounds, and may accelerate wound healing. It is assumed that burned skin that still maintains partial biological activity may be recycled to construct an autologous acellular dermal matrix, termed 'deep‑degree burned dermal matrix (DDBDM)'. In theory, DDBDM may avoid the histoincompatibility issues associated with foreign or xenogeneic dermal matrices, and reduce therapy costs by making full use of discarded skin. In the present study, the collagens within prepared DDBDM were thickened, disorganized and partially fractured, however, they still maintained their reticular structure and tensile strength (P<0.01). Through microarray analysis of the cytokines present in ADM and DDBDM, it was determined that the DDBDM did not produce excessive levels of harmful burn toxins. Following 4 weeks of subcutaneous implantation, ADM and DDBDM were incompletely degraded and maintained good integrity. No significant inflammatory reaction or rejection were observed, which indicated that ADM and DDBDM have good histocompatibility. Therefore, DDBDM may be a useful material for the treatment of deep‑degree burns.
authors: Department of Burns and Plastic Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors: Guanying
authors: G
authors: Yu
authors: Department of Burns and Plastic Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors: Lan
authors: L
authors: Ye
authors: Department of Gastrointestinal Surgery, Jinan Central Hospital, Jinan, Shandong 250013, P.R. China.
authors: Wei
authors: W
authors: Tan
authors: Department of Burns and Plastic Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors: Xuguo
authors: X
authors: Zhu
authors: Department of Burns and Plastic Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors: Yaonan
authors: Y
authors: Li
authors: Department of Burns and Plastic Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
authors: Duyin
authors: D
authors: Jiang
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D002056:Burns / Q000276:immunology / Q000473:pathology / Q000601:surgery*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000166:cytology*; D016038:Skin Transplantation / Q000379:methods; D017768:Skin, Artificial
chemical_list: D016207:Cytokines