title: Core-Shell Electrospun Fibers Encapsulating Chromophores or Luminescent Proteins for Microscopically Controlled Molecular Release.
abstract: Core-shell fibers are emerging as interesting microstructures for the controlled release of drugs, proteins, and complex biological molecules, enabling the fine control of microreservoirs of encapsulated active agents, of the release kinetics, and of the localized delivery. Here we load luminescent molecules and enhanced green fluorescent proteins into the core of fibers realized by coaxial electrospinning. Photoluminescence spectroscopy evidences unaltered molecular emission following encapsulation and release. Moreover, the release kinetics is microscopically investigated by confocal analysis at individual-fiber scale, unveiling different characteristic time scales for diffusional translocation at the core and at the shell. These results are interpreted by a two stage desorption model for the coaxial microstructure, and they are relevant in the design and development of efficient fibrous systems for the delivery of functional biomolecules.
authors: Istituto Nanoscienze-CNR, Euromediterranean Center for Nanomaterial Modelling and Technology (ECMT) , via Arnesano, I-73100 Lecce, Italy.|Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento , via Arnesano, I-73100 Lecce, Italy.
authors: Luigi
authors: L
authors: Romano
authors: Istituto Nanoscienze-CNR, Euromediterranean Center for Nanomaterial Modelling and Technology (ECMT) , via Arnesano, I-73100 Lecce, Italy.
authors: Andrea
authors: A
authors: Camposeo
authors: Istituto Nanoscienze-CNR, Euromediterranean Center for Nanomaterial Modelling and Technology (ECMT) , via Arnesano, I-73100 Lecce, Italy.
authors: Rita
authors: R
authors: Manco
authors: Istituto Nanoscienze-CNR, Euromediterranean Center for Nanomaterial Modelling and Technology (ECMT) , via Arnesano, I-73100 Lecce, Italy.
authors: Maria
authors: M
authors: Moffa
authors: Istituto Nanoscienze-CNR, Euromediterranean Center for Nanomaterial Modelling and Technology (ECMT) , via Arnesano, I-73100 Lecce, Italy.|Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento , via Arnesano, I-73100 Lecce, Italy.
authors: Dario
authors: D
authors: Pisignano
mesh_terms: D004058:Diffusion; D055664:Electrochemical Techniques / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012995:Solubility
chemical_list: D011108:Polymers; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: coaxial electrospinning; controlled release; core−shell fibers; fluorescent proteins; molecular delivery