title: Acute nephrotoxicity of aristolochic acid in vitro: metabolomics study for intracellular metabolic time-course changes.
abstract: Time-course metabolic changes of aristolochic acid nephrotoxicity (AAN) was investigated using acute AAN HK-2 model. And the AAN-related biomarkers were selected. In the results, 11 potential identified biomarkers were selected and validated using multivariate method combined with time-course analysis. Several metabolic pathways, including vitamin metabolism, lipids acalytion, trytophan metabolism and protein degradation were found to be associated with AAN pathology. This research will provide a valuable reference for the discovery of more potential biomarkers of AAN progression in clinic.
authors: a Key Laboratory of Separation Science for Analytical Chemistry, Da Lian Institute of Chemical Physics, Chinese Academy of Science , Dalian , China .|b Graduate School, University of Chinese Academy of Sciences , Beijing , China .
authors: Xiaoyan
authors: X
authors: Liu
authors: c Academy of Integrative Medicine, Dalian Medical University , Dalian , China , and.
authors: Yanqiu
authors: Y
authors: Liu
authors: a Key Laboratory of Separation Science for Analytical Chemistry, Da Lian Institute of Chemical Physics, Chinese Academy of Science , Dalian , China .
authors: Mengchun
authors: M
authors: Cheng
authors: d Systems Analysis and Innovative Drug Research Center, University of Chinese Medicine , Beijing , China.
authors: Hongbin
authors: H
authors: Xiao
mesh_terms: D030022:Aristolochia / Q000009:adverse effects* / Q000737:chemistry; D034341:Aristolochic Acids / Q000008:administration & dosage / Q000009:adverse effects*; D015415:Biomarkers / Q000378:metabolism*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D018450:Disease Progression; D006801:Humans; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000473:pathology; D050356:Lipid Metabolism / Q000187:drug effects; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055432:Metabolomics / Q000379:methods; D014364:Tryptophan / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism
chemical_list: D034341:Aristolochic Acids; D015415:Biomarkers; D014815:Vitamins; D014364:Tryptophan; C000228:aristolochic acid I
keywords: Aristolochic acid nephrotoxicity; HK-2; UPLC-MS; biomarker; metabolomics