title: Lactoferrin Levels in Tears are Increased by the Topical Application of Diadenosine Tetraphosphate.
abstract: This study was undertaken to determine the effect of the topical application of diadenosine tetraphosphate on lactoferrin levels in rabbit tears.
  
  
  
  Diadenosine tetraphosphate was topically instilled in a single-dose, tear samples were collected by micropipette and lactoferrin was measured by Enzyme-Linked ImmunoSorbent Assay (ELISA).
  
  
  
  The concentration of lactoferrin in rabbit tears was significantly increased 1 h after diadenosine tetraphosphate application, remaining elevated for 3 h more. This effect was blocked by P2 receptors antagonists.
  
  
  
  Topical application of diadenosine tetraphosphate stimulates the secretion of lactoferrin in rabbit tears through P2 receptor activation.
authors: a Departamento de Bioquímica y Biología Molecular , E.U. Óptica, Universidad Complutense de Madrid , Madrid , Spain.
authors: Patricia
authors: P
authors: Loma
authors: a Departamento de Bioquímica y Biología Molecular , E.U. Óptica, Universidad Complutense de Madrid , Madrid , Spain.
authors: Ana
authors: A
authors: Guzman-Aranguez
authors: a Departamento de Bioquímica y Biología Molecular , E.U. Óptica, Universidad Complutense de Madrid , Madrid , Spain.
authors: Maria J
authors: MJ
authors: Perez de Lara
authors: a Departamento de Bioquímica y Biología Molecular , E.U. Óptica, Universidad Complutense de Madrid , Madrid , Spain.
authors: Jesus
authors: J
authors: Pintor
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015226:Dinucleoside Phosphates / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay; D007322:Instillation, Drug; D007781:Lactoferrin / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D009883:Ophthalmic Solutions; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage; D011817:Rabbits; D013666:Tears / Q000737:chemistry*
chemical_list: D015226:Dinucleoside Phosphates; D009883:Ophthalmic Solutions; D010975:Platelet Aggregation Inhibitors; C020733:diadenosine tetraphosphate; D007781:Lactoferrin
keywords: Diadenosine tetraphosphate; lactoferrin; purinergic receptors; tear film