title: A polysaccharide isolated from Cynomorium songaricum Rupr. protects PC12 cells against H2O2-induced injury.
abstract: As a great deal of interest is developed to study novel bioactive components with health benefit effects from natural resources, in this paper, a rat pheochromocytoma line 12 (PC12) cell is built to observe the protective effect of a Cynomorium songaricum Rupr. polysaccharide (CSP) against H2O2-induced oxidative stress. Fluorescence microscope, flow cytometry and micro-plate reader are used to assess cell viability and apoptosis. And the levels of reactive oxygen species (ROS), 8-hydroxy-2'-deoxyguanosine (8-OH-dG), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and lactate dehydrogenase (LDH) are evaluated. The results show that, the CSP can significantly protect PC12 cells against H2O2-induced oxidative stress, increase the intracellular antioxidase system load and inhibit H2O2-induced apoptosis by scavenging of ROS, regulating cell cycle, preventing DNA damage and protecting the cell membrane. This research would be benefit for preventing and curing the oxidation-related diseases in polysaccharide study.
authors: Institute of Polymers, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China; Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Lanzhou 730070, China; College of Life Science, Northwest Normal University, Lanzhou 730070, China.
authors: Fengxia
authors: F
authors: Wang
authors: Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Lanzhou 730070, China; College of Life Science, Northwest Normal University, Lanzhou 730070, China.
authors: Qin
authors: Q
authors: Liu
authors: Institute of Polymers, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors: Wei
authors: W
authors: Wang
authors: Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Lanzhou 730070, China; College of Life Science, Northwest Normal University, Lanzhou 730070, China.
authors: Xibo
authors: X
authors: Li
authors: Institute of Polymers, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China; Bioactive Products Engineering Research Center for Gansu Distinctive Plants, Lanzhou 730070, China; College of Life Science, Northwest Normal University, Lanzhou 730070, China. Electronic address: zhangj@nwnu.edu.cn.
authors: Ji
authors: J
authors: Zhang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D031482:Cynomorium / Q000737:chemistry*; D019610:Cytoprotection / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D016716:PC12 Cells; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats
chemical_list: D011134:Polysaccharides; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D006861:Hydrogen Peroxide; D053148:Caspase 3; D053453:Caspase 9; D003849:Deoxyguanosine
keywords: H(2)O(2)-induced injury; PC12 cells; Polysaccharide