title: Elevated interleukin-1β in peripheral blood mononuclear cells contributes to the pathogenesis of autoimmune thyroid diseases, especially of Hashimoto thyroiditis.
abstract: OBJECTIVE
  To explore the relationship between IL-1β expression and two common autoimmune thyroid diseases: Hashimoto thyroiditis (HT) and Graves' disease (GD).
  
  
  METHODS
  qRT-PCR, Quantiglo ELISA, and flow cytometry were used to evaluate the expression levels of IL-1β in serum, peripheral blood mononuclear cells (PBMCs), and thyroid tissue samples from patients with HT or GD. Local infiltration of monocytes was assessed by immunohistochemical study of patients' thyroid tissue samples.
  
  
  RESULTS
  Although no significant differences in IL-1β levels were found between samples of serum from patients with HT or GD and normal controls, we found that IL-1β mRNA and protein levels in PBMCs of HT patients were significantly higher than those of patients with GD, which were in turn higher than the level in normal controls. In addition, IL-1β mRNA was also increased in thyroid gland tissue from patients with HT compared to those with GD, and this was accompanied by increased local infiltration of monocytes into thyroid tissues. Correlation analysis of the clinical samples validated the association of high IL-1β levels with the pathogenesis of HT.
  
  
  CONCLUSIONS
  Our study suggests that IL-1β may be an active etiologic factor in the pathogenesis of HT and thus present a new target for novel diagnostics and treatment.
authors: a Department of Endocrinology , The First Affiliated Hospital of Anhui Medical University , Hefei , Anhui , China.|b Institute of Endocrinology & Metabolism , Anhui Medical University , Hefei , Anhui , China.
authors: Li
authors: L
authors: Sun
authors: a Department of Endocrinology , The First Affiliated Hospital of Anhui Medical University , Hefei , Anhui , China.|b Institute of Endocrinology & Metabolism , Anhui Medical University , Hefei , Anhui , China.
authors: Xiaoxu
authors: X
authors: Zhang
authors: a Department of Endocrinology , The First Affiliated Hospital of Anhui Medical University , Hefei , Anhui , China.|b Institute of Endocrinology & Metabolism , Anhui Medical University , Hefei , Anhui , China.
authors: Fang
authors: F
authors: Dai
authors: c Department of Microbiology and Parasitology , Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Medical University , Hefei , Anhui , China.
authors: Jijia
authors: J
authors: Shen
authors: c Department of Microbiology and Parasitology , Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Medical University , Hefei , Anhui , China.
authors: Cuiping
authors: C
authors: Ren
authors: a Department of Endocrinology , The First Affiliated Hospital of Anhui Medical University , Hefei , Anhui , China.|b Institute of Endocrinology & Metabolism , Anhui Medical University , Hefei , Anhui , China.
authors: Chunlin
authors: C
authors: Zuo
authors: a Department of Endocrinology , The First Affiliated Hospital of Anhui Medical University , Hefei , Anhui , China.|b Institute of Endocrinology & Metabolism , Anhui Medical University , Hefei , Anhui , China.
authors: Qiu
authors: Q
authors: Zhang
mesh_terms: D000328:Adult; D005260:Female; D006111:Graves Disease / Q000097:blood*; D050031:Hashimoto Disease / Q000097:blood*; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: C508585:IL1B protein, human; D053583:Interleukin-1beta
keywords: Autoimmune thyroid diseases; Graves’ disease; Hashimoto thyroiditis; cytokine; interleukin-1β