title: Evaluation of in vitro storage characteristics of cold stored platelet concentrates with N acetylcysteine (NAC).
abstract: Platelets play a vital role in hemostasis and thrombosis, and their demand and usage has multiplied many folds over the years. However, due to the short life span and storage constraints on platelets, it is allowed to store them for up to 7 days at room temperature (RT); thus, there is a need for an alternative storage strategy for extension of shelf life. Current investigation involves the addition of 50 mM N acetylcysteine (NAC) in refrigerated concentrates. Investigation results revealed that addition of NAC to refrigerated concentrates prevented platelet activation and reduced the sialidase activity upon rewarming as well as on prolonged storage. Refrigerated concentrates with 50 mM NAC expressed a 23.91 ± 6.23% of CD62P (P-Selectin) and 22.33 ± 3.42% of phosphotidylserine (PS), whereas RT-stored platelets showed a 46.87 ± 5.23% of CD62P and 25.9 ± 6.48% of phosphotidylserine (PS) after 5 days of storage. Further, key metabolic parameters such as glucose and lactate accumulation indicated reduced metabolic activity. Taken together, investigation and observations indicate that addition of NAC potentially protects refrigerated concentrates by preventing platelet activation, stabilizing sialidase activity, and further reducing the metabolic activity. Hence, we believe that NAC can be a good candidate for an additive solution to retain platelet characteristics during cold storage and may pave the way for extension of storage shelf life.
authors: Department of Laboratory Medicine, Chonbuk National University Medical School and Hospital, Jeonju 561180, Republic of Korea.
authors: Mallikarjun
authors: M
authors: Handigund
authors: Department of Laboratory Medicine, Chonbuk National University Medical School and Hospital, Jeonju 561180, Republic of Korea.
authors: Tae Won
authors: TW
authors: Bae
authors: Department of Laboratory Medicine, Chonbuk National University Medical School and Hospital, Jeonju 561180, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju 561180, Republic of Korea.
authors: Jaehyeon
authors: J
authors: Lee
authors: Department of Laboratory Medicine, Chonbuk National University Medical School and Hospital, Jeonju 561180, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju 561180, Republic of Korea; Chonbuk National University Hospital branch of National Culture Collection for Pathology, Jeonju 561180, Republic of Korea. Electronic address: choyg@jbnu.ac.kr.
authors: Yong Gon
authors: YG
authors: Cho
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005947:Glucose / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009439:Neuraminidase / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects; D010976:Platelet Count; D010979:Platelet Function Tests; D017694:Rewarming; D013997:Time Factors
chemical_list: D015415:Biomarkers; D003451:Cryoprotective Agents; D009439:Neuraminidase; D005947:Glucose; D000111:Acetylcysteine
keywords: CD62P expression; N acetylcysteine; Refrigerated concentrates; Sialidase activity; Storage