title: Allohumibacter endophyticus gen. nov., sp. nov., isolated from the root of wild Artemisia princeps (mugwort).
abstract: A novel actinobacterium designated strain MWE-A11T was isolated from the root of wild Artemisia princeps (mugwort). The isolate was aerobic, Gram-stain-positive and short rod-shaped, and the colonies were yellow and circular with entire margin. Strain MWE-A11T grew at 15-37 °C and pH 6.0-8.0. The predominant isoprenoid quinones were MK-11 and MK-10. The predominant fatty acids were anteiso-C15:0 and iso-C16:0, and the DNA G+C content was 68.8 mol%. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The peptidoglycan contained 2,4-diaminobutyric acid as the diagnostic diamino acid, and the acyl type was glycolyl. Phylogenetic analyses based on 16S rRNA gene sequence comparisons indicated that strain MWE-A11T was affiliated with the family Microbacteriaceae, and was most closely related to the type strains of Humibacter antri (96.4% 16S rRNA gene sequence similarity), Herbiconiux moechotypicola (96.3%), Leifsonia soli (96.3%), Leifsonia lichenia (96.2%), Leifsonia xyli subsp. cynodontis (96.1%), Microbacterium testaceum (96.0%) and Humibacter albus (96.0%). However, the combination of chemotaxonomic properties clearly distinguished strain MWE-A11T from the related taxa at genus level. Accordingly, Allohumibacter endophyticus gen. nov., sp. nov. is proposed to accommodate a new member of the family Microbacteriaceae. The type strain of the type species is MWE-A11T (=JCM 19371T=KCTC 29232T).
authors: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Yu
authors: Y
authors: Ri Kim
authors: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Tae-Su
authors: TS
authors: Kim
authors: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.|Bacterial Resources Research Team, Freshwater Bioresources Research Division, Nakdonggang National Institute of Biological Resources, 137, Donam 2-Gil, Sangju, Gyeongsangbuk-Do, 37242, Republic of Korea.
authors: Ji-Hye
authors: JH
authors: Han
authors: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.|Department of Biology, Inha University, Incheon, 402-751, Republic of Korea.
authors: Yochan
authors: Y
authors: Joung
authors: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Jisun
authors: J
authors: Park
authors: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors: Seung Bum
authors: SB
authors: Kim
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019306:Artemisia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2