title: Costimulation with anti-cluster of differentiation 3 and anti-cluster of differentiation 28 reduces the activity of mucin 1-stimulated human mononuclear cells.
abstract: Cytotoxic T-lymphocyte activation and extension of the cell life span is necessary in order to enable immunotherapy to perform effectively against cancer. In the present study, mucin 1 (MUC1)-stimulated human mononuclear cells (M1SHMCs) were costimulated with bead-attached monoclonal antibodies specific for cluster of differentiation (CD)3 and CD28 receptors. The study was undertaken to determine whether costimulation was capable of enhancing the killing of cancer cellsin vitroand of protecting non-obese diabetic severe combined immunodeficient mice from tumor development. Lysis of MCF-7 tumor cells by M1SHMCs was reduced following costimulation with anti-CD3 and anti-CD28. Furthermore, costimulation with anti-CD3 and anti-CD28 eliminated the protective effects of M1SHMCs on MCF-7 breast cancer cell growth in the non-obese diabetic severe combined immunodeficient mice. The present study suggested that costimulation with anti-CD3 and anti-CD28 is not advisable following antigen activation of lymphocytes under the conditions used here. Using a lower anti-CD3/CD28 bead to T-cell ratio may prevent immune suppression, however, further studies are required to support this hypothesis.
authors: Department of Internal Medicine, Women's Health Research Institute, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA; Department of Microbiology and Immunology, School of Medicine, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA; Department of Biomedical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA; Harrington Cancer Center, Amarillo, TX 79106, USA.
authors: Stephen E
authors: SE
authors: Wright
authors: Department of Internal Medicine, Women's Health Research Institute, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA.
authors: Kathleen A
authors: KA
authors: Rewers-Felkins
authors: Department of Internal Medicine, Women's Health Research Institute, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA.
authors: Imelda
authors: I
authors: Quinlin
authors: Department of Internal Medicine, Women's Health Research Institute, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA.
authors: Fatema
authors: F
authors: Zohra
authors: Department of Internal Medicine, Women's Health Research Institute, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA.
authors: Jewel
authors: J
authors: Ahmed
keywords: anti-cluster of differentiation 28; anti-cluster of differentiation 3; mononuclear cells; mucin 1