title: TiO2 Nanorod Array Constructed Nanotopography for Regulation of Mesenchymal Stem Cells Fate and the Realization of Location-Committed Stem Cell Differentiation.
abstract: As a physical cue for controlling the fate of stem cells, surface nanotopography has attracted much attention to improve the integration between implants and local host tissues and cells. A biocompatible surface TiO2 nanorod array is proposed to regulate the fate of bone marrow derived mesenchymal stem cells (MSCs). TiO2 substrates with different surface nanotopographies: a TiO2 nanorod array and a polished TiO2 ceramic are built by hydrothermal and sintering processes, respectively. The assessment of morphology, viability, gene expression, and protein characterization of the MSCs cultured on the different TiO2 substrates proves that a TiO2 nanorod array promotes the osteogenic differentiation of MSCs, while a TiO2 ceramic with a smooth surface suppresses it. Periodically assembled TiO2 nanorod array stripes on the smooth TiO2 ceramic are constructed by a combination of microfabrication and a chemical synthesis process, which realizes the location-committed osteogenic differentiation of MSCs. A route to control the differentiation of MSCs by a nanostructured surface, which can also control the location and direction of MSCs on the surface of biomaterials with micro-nano scale surface engineering, is demonstrated.
authors: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
authors: Jichuan
authors: J
authors: Qiu
authors: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
authors: Jianhua
authors: J
authors: Li
authors: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST), Beijing, 100083, P. R. China.
authors: Shu
authors: S
authors: Wang
authors: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
authors: Baojin
authors: B
authors: Ma
authors: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
authors: Shan
authors: S
authors: Zhang
authors: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST), Beijing, 100083, P. R. China.
authors: Weibo
authors: W
authors: Guo
authors: Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST), Beijing, 100083, P. R. China.
authors: Xiaodi
authors: X
authors: Zhang
authors: Department of Pathogenic Biology, School of Medicine, Shandong University, Jinan, 250012, P. R. China.
authors: Wei
authors: W
authors: Tang
authors: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.
authors: Yuanhua
authors: Y
authors: Sang
authors: State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, P. R. China.|Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, National Center for Nanoscience and Technology (NCNST), Beijing, 100083, P. R. China.
authors: Hong
authors: H
authors: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects; D017208:Rats, Wistar; D014025:Titanium / Q000494:pharmacology*
chemical_list: C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: nanorods; nanotopography; self-renewal; stem cell differentiation; stem cells