title: Comparison of New Preservation Solutions, HN-1 and University of Wisconsin Solution, in Pancreas Preservation for Porcine Islet Isolation.
abstract: For pancreatic islet transplantation, maintaining organ viability after pancreas procurement is critical and a major determinant for better graft function and survival. University of Wisconsin (UW) solution is currently the gold standard for abdominal organ preservation and the pancreas in particular. However, in the use of UW preservation solution for islet transplantation, there are disadvantages to be overcome, such as the inhibition of collagenase activity during pancreatic digestion. In this study, we compared UW solution with HN-1 solution in pancreas preservation for islet isolation. Islet yield was significantly greater in the HN-1 group than the UW group both before and after purification. In the in vitro assay, the adenosine triphosphate content in cultured islets was significantly higher in the HN-1 group than in the UW group. Furthermore, in streptozotocin-induced diabetic nude mice, the islet graft function of the HN-1 group was superior to that of the UW group. We concluded that the use of HN-1 solution is a promising approach for optimal pancreas preservation in islet transplantation.
authors: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama 700-8558 , Japan.
authors: Akihiro
authors: A
authors: Katayama
authors: † Department of Surgery, Chiba-East National Hospital, National Hospital Organization , Chiba 260-8712 , Japan.
authors: Hirofumi
authors: H
authors: Noguchi
authors: ‡ Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Takashi
authors: T
authors: Kuise
authors: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama 700-8558 , Japan.
authors: Atsuko
authors: A
authors: Nakatsuka
authors: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama 700-8558 , Japan.
authors: Daisho
authors: D
authors: Hirota
authors: § Department of Primary Care and Medical Education, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Hitomi Usui
authors: HU
authors: Kataoka
authors: ‡ Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Takashi
authors: T
authors: Kawai
authors: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama 700-8558 , Japan.
authors: Kentaro
authors: K
authors: Inoue
authors: ‡ Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Noriko
authors: N
authors: Imagawa
authors: ¶ Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Niigata , Japan.
authors: Issei
authors: I
authors: Saitoh
authors: # Department of Socio-environmental Design, Hiroshima International University , Hiroshima , Japan.
authors: Yasufumi
authors: Y
authors: Noguchi
authors: * Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Masami
authors: M
authors: Watanabe
authors: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama 700-8558 , Japan.
authors: Jun
authors: J
authors: Wada
authors: ‡ Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Toshiyoshi
authors: T
authors: Fujiwara
keywords: HN-1 solution; Islet isolation; Islet transplantation; Preservation; University of Wisconsin (UW) solution