title: Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC).
abstract: Short peptide tags genetically fused to recombinant proteins have been widely used to facilitate detection or purification without the need to develop specific procedures. In general, an ideal affinity tag would allow the efficient purification of tagged proteins in high yield, without affecting its function. Here, we describe the purification steps to purify a recombinant polyhistidine-tagged glucosyltransferase from Centella asiatica using immobilized metal affinity chromatography.
authors: Plant Physiology Laboratory, Department of Botany, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Porto Alegre, RS, 91501-970, Brazil.|Center for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors: Fernanda
authors: F
authors: de Costa
authors: National Research Council of Canada, 110 Gymnasium Pl., Saskatoon, SK, Canada, S7N 0W9.
authors: Carla J S
authors: CJ
authors: Barber
authors: National Research Council of Canada, 110 Gymnasium Pl., Saskatoon, SK, Canada, S7N 0W9.
authors: Pareshkumar T
authors: PT
authors: Pujara
authors: National Research Council of Canada, 110 Gymnasium Pl., Saskatoon, SK, Canada, S7N 0W9.
authors: Darwin W
authors: DW
authors: Reed
authors: National Research Council of Canada, 110 Gymnasium Pl., Saskatoon, SK, Canada, S7N 0W9. patrick.covello@nrc-cnrc.gc.ca.
authors: Patrick S
authors: PS
authors: Covello
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D005964:Glucosyltransferases / Q000235:genetics / Q000302:isolation & purification*; D006639:Histidine / Q000235:genetics; D008670:Metals / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D008670:Metals; D011993:Recombinant Fusion Proteins; C033223:polyhistidine; D006639:Histidine; D005964:Glucosyltransferases
keywords: Glucosyltransferase; His-tagged protein; IMAC; Purification; Recombinant