title: Pyruvate dehydrogenase B promoted the growth and migration of the nasopharyngeal carcinoma cells.
abstract: Metabolism alteration is one of the hallmarks of cancer cells. Although several studies have demonstrated that glycolysis played important roles in the progression of nasopharyngeal carcinoma (NPC), the functions of specific metabolism-associated genes remain largely unknown. In this study, it was found that Pyruvate dehydrogenase B (PDHB), which catalyzed the conversion of pyruvate to Acetyl-CoA, was downregulated in NPC cells. Forced expression of PDHB in NPC cells inhibited cell growth and migration, while knocking down the expression of PDHB promoted the growth, migration, and tumorigenesis of NPC cells. Mechanism study showed that PDHB inhibited ERK signaling and cell growth driven by RasV12. Collectively, our study demonstrated the suppressive roles of PDHB in the progression of NPC, and restoring the function of PDHB might be a promising strategy for NPC therapy.
authors: Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of University of South China, Hengyang, Hunan, 421001, People's Republic China.
authors: Hongbo
authors: H
authors: Tang
authors: Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of University of South China, Hengyang, Hunan, 421001, People's Republic China.
authors: Xinggu
authors: X
authors: Luo
authors: Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of University of South China, Hengyang, Hunan, 421001, People's Republic China.
authors: Juan
authors: J
authors: Li
authors: State Key Laboratory of Respiratory Disease, Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of Guangzhou Medical University, 151# West Yanjiang Road, Guangzhou, Guangdong, 510120, People's Republic China.
authors: Yi
authors: Y
authors: Zhou
authors: State Key Laboratory of Respiratory Disease, Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of Guangzhou Medical University, 151# West Yanjiang Road, Guangzhou, Guangdong, 510120, People's Republic China.
authors: Yanmei
authors: Y
authors: Li
authors: State Key Laboratory of Respiratory Disease, Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of Guangzhou Medical University, 151# West Yanjiang Road, Guangzhou, Guangdong, 510120, People's Republic China.
authors: Lijuan
authors: L
authors: Song
authors: State Key Laboratory of Respiratory Disease, Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of Guangzhou Medical University, 151# West Yanjiang Road, Guangzhou, Guangdong, 510120, People's Republic China. entxiaowen@163.com.
authors: Xiaowen
authors: X
authors: Zhang
authors: State Key Laboratory of Respiratory Disease, Department of Otolaryngology‑Head and Neck Surgery, First Affiliated Hospital of Guangzhou Medical University, 151# West Yanjiang Road, Guangzhou, Guangdong, 510120, People's Republic China. taochen@gird.cn.
authors: Tao
authors: http://orcid.org/0000-0002-8433-2509
authors: T
authors: Chen
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000201:enzymology / Q000473:pathology*; D002455:Cell Division; D045744:Cell Line, Tumor; D002465:Cell Movement; D055785:Gene Knockdown Techniques; D006019:Glycolysis; D064593:Heterografts; D006801:Humans; D020935:MAP Kinase Signaling System; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000201:enzymology / Q000473:pathology*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D016283:Proto-Oncogene Proteins p21(ras) / Q000502:physiology; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D030481:Pyruvate Dehydrogenase (Lipoamide); D016283:Proto-Oncogene Proteins p21(ras)
keywords: Cell growth and migration; NPC; PDHB; Ras