title: Effect of Concentrated Fibroblast-Conditioned Media on In Vitro Maintenance of Rat Primary Hepatocyte.
abstract: The effects of concentrated fibroblast-conditioned media were tested to determine whether hepatocyte function can be maintained without direct contact between hepatocytes and fibroblasts. Primary rat hepatocytes cultured with a concentrated conditioned media of NIH-3T3 J2 cell line (final concentration of 55 mg/ml) showed significantly improved survival and functions (albumin and urea) compared to those of control groups. They also showed higher expression levels of mRNA, albumin and tyrosine aminotransferase compared to hepatocyte monoculture. The results suggest that culture with concentrated fibroblast-conditioned media could be an easy method for in vitro maintenance of primary hepatocytes. They also could be contribute to understand and analyze co-culture condition of hepatocyte with stroma cells.
authors: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.
authors: Dayeong
authors: D
authors: Jeong
authors: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.
authors: Chungmin
authors: C
authors: Han
authors: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.
authors: Inhye
authors: I
authors: Kang
authors: Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.
authors: Hyun Taek
authors: HT
authors: Park
authors: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.
authors: Jiyoon
authors: J
authors: Kim
authors: Chadwick International School, Songdo, Incheon, Republic of Korea.
authors: Hayoung
authors: H
authors: Ryu
authors: Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.
authors: Yong Song
authors: YS
authors: Gho
authors: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.|Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeong-buk, Republic of Korea.
authors: Jaesung
authors: J
authors: Park
mesh_terms: D016475:3T3 Cells / Q000378:metabolism*; D000418:Albumins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D005260:Female; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016133:Polymerase Chain Reaction; D061251:Primary Cell Culture / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells; D014508:Urea / Q000378:metabolism
chemical_list: D000418:Albumins; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D014508:Urea