title: Thyroid hormone responsive (THRSP) promotes the synthesis of medium-chain fatty acids in goat mammary epithelial cells.
abstract: In nonruminants, thyroid hormone responsive (THRSP) is a crucial protein for cellular de novo lipogenesis. However, the role of THRSP in regulating the synthesis of milk fatty acid composition in goat mammary gland remains unknown. In the present study, we compared gene expression of THRSP among different goat tissues. Results revealed that THRSP had the highest expression in subcutaneous fat, and expression was higher during lactation compared with the dry period. Overexpression of THRSP upregulated the expression of fatty acid synthase (FASN), stearoyl-coenzyme A desaturase 1 (SCD1), diacylglycerol acyltransferase 2 (DGAT2), and glycerol-3-phosphate acyltransferase (GPAM) in goat mammary epithelial cells. In contrast, overexpression of THRSP led to downregulation of thrombospondin receptor (CD36) and had no effect on the expression of acetyl-coenzyme A carboxylase α (ACACA) and sterol regulatory element binding transcription factor1 (SREBF1). In addition, overexpressing THRSP in vitro resulted in a significant increase in triacylglycerol (TAG) concentration and the concentrations of C12:0 and C14:0. Taken together, these results highlight an important role of THRSP in regulating lipogenesis in goat mammary epithelial cells.
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 712100.
authors: D W
authors: DW
authors: Yao
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 712100. Electronic address: luojun@nwsuaf.edu.cn.
authors: J
authors: J
authors: Luo
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 712100.
authors: Q Y
authors: QY
authors: He
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 712100.
authors: M
authors: M
authors: Wu
authors: College of Animal Sciences, Zhejiang Sci-Tech University, Hangzhou, P. R. China 310058.
authors: H B
authors: HB
authors: Shi
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 712100.
authors: H
authors: H
authors: Wang
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 712100.
authors: M
authors: M
authors: Wang
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 712100.
authors: H F
authors: HF
authors: Xu
authors: Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana 61801. Electronic address: jloor@illinois.edu.
authors: J J
authors: JJ
authors: Loor
mesh_terms: D000214:Acyl Coenzyme A / Q000235:genetics; D000818:Animals; D018955:CD36 Antigens / Q000235:genetics; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D006041:Goats / Q000378:metabolism*; D007774:Lactation; D050155:Lipogenesis / Q000235:genetics; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000378:metabolism*; D008892:Milk / Q000737:chemistry; D009687:Nuclear Proteins / Q000502:physiology; D013963:Thyroid Hormones; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014280:Triglycerides / Q000032:analysis
chemical_list: D000214:Acyl Coenzyme A; D018955:CD36 Antigens; D005227:Fatty Acids; D009687:Nuclear Proteins; C051065:THRSP protein, human; D013963:Thyroid Hormones; D014157:Transcription Factors; D014280:Triglycerides; C017725:stearoyl-coenzyme A; D064429:Fatty Acid Synthases
keywords: dairy nutrition; fatty acid composition; goat; milk fat