title: Overexpression of thyroid hormone receptor β1 altered thyroid hormone-mediated regulation of herpes simplex virus-1 replication in differentiated cells.
abstract: Thyroid hormone (T3) has been suggested to play a role in herpes simplex virus 1 (HSV-1) replication. It was previously reported that HSV-1 replication was suppressed by T3in mouse neuroblastoma cells overexpressing thyroid hormone receptor β1 (TRβ1). Using a human neuro-endocrine cells LNCaP differentiated by androgen deprivation, HSV-1 replication was active but decreased by T3at very low moi, probably due to low copy of TRβ1. In this study, a recombinant HSV-1 was constructed expressing TRβ1 (HSV-1/TRβ1). Infection of Vero cells (very little TRβ1 expression) with HSV-1/TRβ1 exhibited increased replication in the presence of T3compared to the counterpart without TRβ1 overexpression. Interestingly, HSV-1/TRβ1 infection of differentiated LNCaP cells showed strong suppression of viral replication by T3and the removal of hormone did not fully reversed the suppression as was observed in parent virus. Quantitative analyses indicated that ICP0 expression was blocked using HSV-1/TRβ1 for infection during T3washout, suggesting that overexpression of TRβ1 is likely to delay its inhibitory effect on viral gene expression. Together these results emphasized the importance of TRβ1 in the regulation of HSV-1 replication in differentiated environment with neuronal phenotype.
authors: Department of Pharmaceutical Sciences, School of Pharmacy and Health Professions, University of Maryland Eastern Shore, Princess Anne, MD, USA.
authors: Feng
authors: F
authors: Chen
authors: Department of Pharmaceutical Sciences, School of Pharmacy and Health Professions, University of Maryland Eastern Shore, Princess Anne, MD, USA.|Department of Natural Sciences, School of Agriculture and Natural Sciences, University of Maryland Eastern Shore, Princess Anne, MD, USA.
authors: Robert W
authors: RW
authors: Figliozzi
authors: Department of Pharmaceutical Sciences, School of Pharmacy and Health Professions, University of Maryland Eastern Shore, Princess Anne, MD, USA.|Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA, USA.
authors: Gautam
authors: G
authors: Bedadala
authors: Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA, USA.
authors: Jayavardhana
authors: J
authors: Palem
authors: Department of Pharmaceutical Sciences, School of Pharmacy and Health Professions, University of Maryland Eastern Shore, Princess Anne, MD, USA. vhsia@umes.edu.|Department of Natural Sciences, School of Agriculture and Natural Sciences, University of Maryland Eastern Shore, Princess Anne, MD, USA. vhsia@umes.edu.
authors: S Victor
authors: SV
authors: Hsia
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D018628:Gene Dosage; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000821:virology; D037042:Thyroid Hormone Receptors beta / Q000235:genetics* / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D017874:Immediate-Early Proteins; D037042:Thyroid Hormone Receptors beta; D014284:Triiodothyronine; D049452:Green Fluorescent Proteins; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1
keywords: Differentiation; Herpes simplex virus; Neurons; Plaque assay; Thyroid hormone; Thyroid hormone receptor beta 1