title: Lateral Root Inducible System in Arabidopsis and Maize.
abstract: Lateral root development contributes significantly to the root system, and hence is crucial for plant growth. The study of lateral root initiation is however tedious, because it occurs only in a few cells inside the root and in an unpredictable manner. To circumvent this problem, a Lateral Root Inducible System (LRIS) has been developed. By treating seedlings consecutively with an auxin transport inhibitor and a synthetic auxin, highly controlled lateral root initiation occurs synchronously in the primary root, allowing abundant sampling of a desired developmental stage. The LRIS has first been developed for Arabidopsis thaliana, but can be applied to other plants as well. Accordingly, it has been adapted for use in maize (Zea mays). A detailed overview of the different steps of the LRIS in both plants is given. The combination of this system with comparative transcriptomics made it possible to identify functional homologs of Arabidopsis lateral root initiation genes in other species as illustrated here for the CYCLIN B1;1 (CYCB1;1) cell cycle gene in maize. Finally, the principles that need to be taken into account when an LRIS is developed for other plant species are discussed.
authors: Department of Plant Systems Biology, VIB, Ghent; Department of Plant Biotechnology and Bioinformatics, Ghent University.
authors: Hanne
authors: H
authors: Crombez
authors: Department of Plant Systems Biology, VIB, Ghent; Department of Plant Biotechnology and Bioinformatics, Ghent University.
authors: Ianto
authors: I
authors: Roberts
authors: Department of Plant Systems Biology, VIB, Ghent; Department of Plant Biotechnology and Bioinformatics, Ghent University.
authors: Nick
authors: N
authors: Vangheluwe
authors: Department of Plant Systems Biology, VIB, Ghent; Department of Plant Biotechnology and Bioinformatics, Ghent University.
authors: Hans
authors: H
authors: Motte
authors: Department of Plant Systems Biology, VIB, Ghent; Department of Plant Biotechnology and Bioinformatics, Ghent University.
authors: Leentje
authors: L
authors: Jansen
authors: Department of Plant Systems Biology, VIB, Ghent; Department of Plant Biotechnology and Bioinformatics, Ghent University; tobee@psb.vib-ugent.be.
authors: Tom
authors: T
authors: Beeckman
authors: Department of Plant Systems Biology, VIB, Ghent; Department of Plant Biotechnology and Bioinformatics, Ghent University.
authors: Boris
authors: B
authors: Parizot
mesh_terms: D017360:Arabidopsis / Q000254:growth & development*; D001692:Biological Transport; D007210:Indoleacetic Acids / Q000494:pharmacology; D063245:Plant Development; D018517:Plant Roots / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development*; D003313:Zea mays / Q000254:growth & development*
chemical_list: D007210:Indoleacetic Acids