title: Aptamer and Antisense-Mediated Two-Dimensional Isolation of Specific Cancer Cell Subpopulations.
abstract: Cancer cells, and in particular those found circulating in blood, can have widely varying phenotypes and molecular profiles despite a common origin. New methods are needed that can deconvolute the heterogeneity of cancer cells and sort small numbers of cells to aid in the characterization of cancer cell subpopulations. Here, we describe a new molecular approach to capturing cancer cells that isolates subpopulations using two-dimensional sorting. Using aptamer-mediated capture and antisense-triggered release, the new strategy sorts cells according to levels of two different markers and thereby separates them into their corresponding subpopulations. Using a phenotypic assay, we demonstrate that the subpopulations isolated have markedly different properties. This system provides an important new tool for identifying circulating tumor cell subtypes.
authors: Department of Pharmaceutical Sciences, University of Toronto , Toronto, Ontario M5S 3M2, Canada.
authors: Mahmoud
authors: M
authors: Labib
authors: Institute for Biomedical and Biomaterials Engineering, University of Toronto , Toronto, Ontario M5S 3G4, Canada.
authors: Brenda
authors: B
authors: Green
authors: Department of Pharmaceutical Sciences, University of Toronto , Toronto, Ontario M5S 3M2, Canada.
authors: Reza M
authors: RM
authors: Mohamadi
authors: Institute for Biomedical and Biomaterials Engineering, University of Toronto , Toronto, Ontario M5S 3G4, Canada.
authors: Adam
authors: A
authors: Mepham
authors: Department of Pharmaceutical Sciences, University of Toronto , Toronto, Ontario M5S 3M2, Canada.
authors: Sharif U
authors: SU
authors: Ahmed
authors: Department of Pharmaceutical Sciences, University of Toronto , Toronto, Ontario M5S 3M2, Canada.
authors: Laili
authors: L
authors: Mahmoudian
authors: Department of Pharmaceutical Sciences, University of Toronto , Toronto, Ontario M5S 3M2, Canada.
authors: I-Hsin
authors: IH
authors: Chang
authors: Edward H
authors: EH
authors: Sargent
authors: Department of Pharmaceutical Sciences, University of Toronto , Toronto, Ontario M5S 3M2, Canada.|Institute for Biomedical and Biomaterials Engineering, University of Toronto , Toronto, Ontario M5S 3G4, Canada.
authors: Shana O
authors: SO
authors: Kelley
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000737:chemistry* / Q000235:genetics; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000097:blood / Q000145:classification / Q000235:genetics / Q000473:pathology*; D009360:Neoplastic Cells, Circulating / Q000145:classification / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D016373:DNA, Antisense; D004273:DNA, Neoplasm