title: The angiogenic variation of skeletal site-specific human BMSCs from same alveolar cleft patients: a comparative study.
abstract: Tissue engineering strategies hold great potential for alveolar cleft reconstruction. Bone marrow stromal cells (BMSCs) from iliac crest and craniofacial regions are candidate seeding cells with site-specific characteristics and bone-repairing properties. Craniofacial BMSCs seem to possess stronger multipotency and osteogenic capabilities than BMSCs isolated from iliac crest. However, the angiogenic capabilities of these two type cell is rarely reported. We obtained human BMSCs (hBMSCs) of maxilla (M-hBMSCs) and iliac crest (I-hBMSCs) from same alveolar cleft patients to investigate the agiogenic variations using co-culture system with human umbilical vein endothelial cells (HUVECs). From in vitro comparison, M-hBMSCs allowed HUVECs to form more tube-like structures and sprouting angiogenesis by tube formation assays and 3D fibrin vasculogenic assay, respectively. By transplantation in vivo, M-hBMSCs enhanced larger size vessel like structures distributed the entire implants compared with I-hBMSCs. Western blotting was used to assess the angiogenesis related factors including hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). The results showed a significant higher expression of bFGF protein in M-hBMSCs and HUVECs co-culture system both in vitro and in vivo. As bFGF could promote migration and proliferation of endothelial cells, scratch wound healing and transwell migration assays were performed as well as MTT assays and cell cycle analysis. The data suggested the effect of M-hBMSCs on HUVECs was stronger than I-hBMSCs. Taken together, these results indicated that craniofacial BMSCs seemed to have greater angiogenesis capability than iliac crest BMSCs and this might be associated with the different levels of bFGF protein expression in co-culture system.
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Yifei
authors: Y
authors: Du
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Fei
authors: F
authors: Jiang
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Yi
authors: Y
authors: Liang
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Yuli
authors: Y
authors: Wang
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Polyclinic Dentistry, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Weina
authors: W
authors: Zhou
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Orthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Yongchu
authors: Y
authors: Pan
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Mingfei
authors: M
authors: Xue
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Yan
authors: Y
authors: Peng
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China.
authors: Huan
authors: H
authors: Yuan
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. danxiangren2006@163.com.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. danxiangren2006@163.com.
authors: Ning
authors: N
authors: Chen
authors: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Number 136, Hanzhong Road, Nanjing, 210029, People's Republic of China. jhbcd@sina.com.|Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, People's Republic of China. jhbcd@sina.com.
authors: Hongbing
authors: H
authors: Jiang
mesh_terms: D050156:Adipogenesis; D000293:Adolescent; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D002971:Cleft Lip / Q000473:pathology*; D018920:Coculture Techniques; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D008297:Male; D008437:Maxilla / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018919:Neovascularization, Physiologic; D010012:Osteogenesis
chemical_list: D016222:Fibroblast Growth Factor 2
keywords: Alveolar cleft graft; Angiogenesis; Basic fibroblast growth factor (bFGF); Co-culture; Human bone marrow mesenchymal stem cells (hBMSCs); Site-specific characteristic