title: Acute and short-term developmental toxicity of cyhalofop-butyl to zebrafish (Danio rerio).
abstract: Cyhalofop-butyl is an aryloxyphenoxypropionate post-emergence herbicide widely used around the world in agriculture. The acute toxicity of cyhalofop-butyl to embryos, larvae (12 and 72 h post-hatching), and adult zebrafish, as well as the short-term developmental toxicity of cyhalofop-butyl to embryo and sac-fry stages, was tested. The results showed that the 96-h LC50 values of cyhalofop-butyl to embryos, 12 h post-hatching larvae, 72 h post-hatching larvae, and adult fish were 2.03, 0.58, 1.42, and 3.49 mg/L, respectively, suggesting zebrafish early life stages were more sensitive to cyhalofop-butyl than adult stage. Cyhalofop-butyl would inhibit the spontaneous movement, heartbeat, hatching rate of embryos, and the body length of surviving larvae of zebrafish at 1.00 mg/L or higher concentrations. Morphological abnormalities, including pericardial edema, yolk sac edema, deformation of tail, and deformation of spine, were induced by cyhalofop-butyl. The results indicated that cyhalofop-butyl had significant negative impacts on zebrafish at different life stages, and spontaneous movement and hatching rate were sensitive endpoints for assessing short-term developmental toxicity of cyhalofop-butyl.
authors: College of Sciences, China Agricultural University, Beijing, 100193, China.
authors: Fangjie
authors: F
authors: Cao
authors: College of Sciences, China Agricultural University, Beijing, 100193, China.
authors: Xiaoshan
authors: X
authors: Liu
authors: College of Sciences, China Agricultural University, Beijing, 100193, China.
authors: Chengju
authors: C
authors: Wang
authors: College of Sciences, China Agricultural University, Beijing, 100193, China.
authors: Mingqi
authors: M
authors: Zheng
authors: College of Sciences, China Agricultural University, Beijing, 100193, China.
authors: Xuefeng
authors: X
authors: Li
authors: College of Sciences, China Agricultural University, Beijing, 100193, China. lihongqiuyang@126.com.
authors: Lihong
authors: L
authors: Qiu
mesh_terms: D000818:Animals; D002073:Butanes / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D006540:Herbicides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects* / Q000502:physiology; D007928:Lethal Dose 50; D008297:Male; D009068:Movement / Q000187:drug effects; D009570:Nitriles / Q000633:toxicity*; D023382:Toxicity Tests, Acute; D015027:Zebrafish / Q000196:embryology / Q000254:growth & development / Q000502:physiology
chemical_list: D002073:Butanes; D006540:Herbicides; D009570:Nitriles; C501583:cyhalofop-butyl
keywords: Acute toxicity; Cyhalofop-butyl; Developmental toxicity; Sensitive endpoints; Sub-lethal concentrations; Zebrafish