title: A Potential of an Anti-HTLV-I gp46 Neutralizing Monoclonal Antibody (LAT-27) for Passive Immunization against Both Horizontal and Mother-to-Child Vertical Infection with Human T Cell Leukemia Virus Type-I.
abstract: Although the number of human T-cell leukemia virus type-I (HTLV-I)-infected individuals in the world has been estimated at over 10 million, no prophylaxis vaccines against HTLV-I infection are available. In this study, we took a new approach for establishing the basis of protective vaccines against HTLV-I. We show here the potential of a passively administered HTLV-I neutralizing monoclonal antibody of rat origin (LAT-27) that recognizes epitopes consisting of the HTLV-I gp46 amino acids 191-196. LAT-27 completely blocked HTLV-I infection in vitro at a minimum concentration of 5 μg/mL. Neonatal rats born to mother rats pre-infused with LAT-27 were shown to have acquired a large quantity of LAT-27, and these newborns showed complete resistance against intraperitoneal infection with HTLV-I. On the other hand, when humanized immunodeficient mice were pre-infused intravenously with humanized LAT-27 (hu-LAT-27), all the mice completely resisted HTLV-I infection. These results indicate that hu-LAT-27 may have a potential for passive immunization against both horizontal and mother-to-child vertical infection with HTLV-I.
authors: Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa 903-0215, Japan. hfujii@med.u-ryukyu.ac.jp.
authors: Hideki
authors: H
authors: Fujii
authors: IBL (Immuno-Biological Laboratories Co., Ltd.), Naka 1091-1, Fujioka, Gunma 375-0005, Japan. do-shimizu@ibl-japan.co.jp.
authors: Mamoru
authors: M
authors: Shimizu
authors: Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa 903-0215, Japan. miya_skywalker2008@yahoo.co.jp.
authors: Takuya
authors: T
authors: Miyagi
authors: Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa 903-0215, Japan. k138751@eve.u-ryukyu.ac.jp.
authors: Marie
authors: M
authors: Kunihiro
authors: Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa 903-0215, Japan. reiko_tanaka@s5.dion.ne.jp.
authors: Reiko
authors: R
authors: Tanaka
authors: Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa 903-0215, Japan. ytakah3@med.u-ryukyu.ac.jp.
authors: Yoshiaki
authors: Y
authors: Takahashi
authors: Department of Immunology, Graduate School of Medicine, University of the Ryukyus, Uehara 207, Nishihara-cho, Okinawa 903-0215, Japan. yuetsu@s4.dion.ne.jp.
authors: Yuetsu
authors: Y
authors: Tanaka
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology*; D018562:Disease Transmission, Infectious / Q000517:prevention & control*; D005260:Female; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D015490:HTLV-I Infections / Q000276:immunology / Q000517:prevention & control* / Q000635:transmission* / Q000821:virology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007116:Immunization, Passive; D007223:Infant; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control*; D008297:Male; D051379:Mice; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015686:Gene Products, env; D015682:Retroviridae Proteins, Oncogenic; C055266:gp46 protein, Human T-cell leukemia virus type I
keywords: HTLV-I; NOG mice; envelope gp46; neutralizing monoclonal antibody; passive immunity