title: Mechanisms of airway responses to esophageal acidification in cats.
abstract: Acid in the esophagus causes airway constriction, tracheobronchial mucous secretion, and a decrease in tracheal mucociliary transport rate. This study was designed to investigate the neuropharmacological mechanisms controlling these responses. In chloralose-anesthetized cats (n = 72), we investigated the effects of vagotomy or atropine (100 μg·kg(-1)·30 min(-1) iv) on airway responses to esophageal infusion of 0.1 M PBS or 0.1 N HCl at 1 ml/min. We quantified 1) diameter of the bronchi, 2) tracheobronchial mucociliary transport rate, 3) tracheobronchial mucous secretion, and 4) mucous content of the tracheal epithelium and submucosa. We found that vagotomy or atropine blocked the airway constriction response but only atropine blocked the increase in mucous output and decrease in mucociliary transport rate caused by esophageal acidification. The mucous cells of the mucosa produced more Alcian blue- than periodic acid-Schiff (PAS)-stained mucosubstances, and the mucous cells of the submucosa produced more PAS- than Alcian blue-stained mucosubstances. Selective perfusion of the different segments of esophagus with HCl or PBS resulted in significantly greater production of PAS-stained mucus in the submucosa of the trachea adjacent to the HCl-perfused esophagus than in that adjacent to the PBS-perfused esophagus. In conclusion, airway constriction caused by esophageal acidification is mediated by a vagal cholinergic pathway, and the tracheobronchial transport response is mediated by cholinergic receptors. Acid perfusion of the esophagus selectively increases production of neutral mucosubstances of the apocrine glands by a local mechanism. We hypothesize that the airway responses to esophageal acid exposure are part of the innate, rather than acute emergency, airway defense system.
authors: Dysphagia Institute, Division of Gastroenterology and Hepatology, Medical College of Wisconsin, Milwaukee, Wisconsin; imlang@mcw.edu.
authors: Ivan M
authors: IM
authors: Lang
authors: Department of Pulmonary Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin; and.
authors: Steven T
authors: ST
authors: Haworth
authors: Dysphagia Institute, Division of Gastroenterology and Hepatology, Medical College of Wisconsin, Milwaukee, Wisconsin;
authors: Bidyut K
authors: BK
authors: Medda
authors: Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin.
authors: Hubert
authors: H
authors: Forster
authors: Dysphagia Institute, Division of Gastroenterology and Hepatology, Medical College of Wisconsin, Milwaukee, Wisconsin;
authors: Reza
authors: R
authors: Shaker
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D002415:Cats; D004947:Esophagus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005260:Female; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009093:Mucus / Q000187:drug effects / Q000378:metabolism; D010477:Perfusion / Q000379:methods; D014132:Trachea / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D014628:Vagotomy / Q000379:methods; D014630:Vagus Nerve / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
chemical_list: D001285:Atropine
keywords: acid; airway; esophagus; mucus; vagus nerve