title: Potent In Vitro and In Vivo Activity of Plantibody Specific for Porphyromonas gingivalis FimA.
abstract: Fimbrial protein fimbrillin (FimA), a major structural subunit of Porphyromonas gingivalis, has been suggested as a vaccine candidate to control P. gingivalis-induced periodontal disease. Previously, cDNAs encoding IgG monoclonal antibodies (MAbs) against purified FimA from P. gingivalis 2561 have been cloned, and the MAbs have been produced in rice cell suspension. Here we examined the biological activities of the plant-produced MAb specific for FimA (anti-FimA plantibody) of P. gingivalis in vitro and in vivo. The anti-FimA plantibody recognized oligomeric/polymeric forms of native FimA in immunoblot analysis and showed high affinity for native FimA (KD = 0.11 nM). Binding of P. gingivalis (10(8) cells) to 2 mg of saliva-coated hydroxyapatite beads was reduced by 53.8% in the presence of 1 μg/ml plantibody. Anti-FimA plantibody (10 μg/ml) reduced invasion of periodontal ligament cells by P. gingivalis (multiplicity of infection, 100) by 68.3%. Intracellular killing of P. gingivalis opsonized with the anti-FimA plantibody by mouse macrophages was significantly increased (77.1%) compared to killing of bacterial cells with irrelevant IgG (36.7%). In a mouse subcutaneous chamber model, the number of recoverable P. gingivalis cells from the chamber fluid was significantly reduced when the numbers of bacterial cells opsonized with anti-FimA plantibody were compared with the numbers of bacterial cells with irrelevant IgG, 66.7% and 37.1%, respectively. These in vitro and in vivo effects of anti-FimA plantibody were comparable to those of the parental MAb. Further studies with P. gingivalis strains with different types of fimbriae are needed to investigate the usefulness of anti-FimA plantibody for passive immunization to control P. gingivalis-induced periodontal disease.
authors: Department of Maxillofacial Biomedical Engineering, School of Dentistry, and Institute of Oral Biology, Kyung Hee University, Seoul, Republic of Korea Department of Dental Hygiene, Shinsung University, Chungnam, Republic of Korea.
authors: Young-Suk
authors: YS
authors: Choi
authors: Department of Maxillofacial Biomedical Engineering, School of Dentistry, and Institute of Oral Biology, Kyung Hee University, Seoul, Republic of Korea Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, Republic of Korea.
authors: Ji-Hoi
authors: http://orcid.org/0000-0003-0286-5297
authors: JH
authors: Moon
authors: Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Republic of Korea.
authors: Tae-Geum
authors: TG
authors: Kim
authors: Department of Maxillofacial Biomedical Engineering, School of Dentistry, and Institute of Oral Biology, Kyung Hee University, Seoul, Republic of Korea ljinyong@khu.ac.kr.
authors: Jin-Yong
authors: JY
authors: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D058491:Bacterial Load; D016866:Bacteroidaceae Infections / Q000382:microbiology / Q000517:prevention & control; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D036781:Fimbriae Proteins / Q000276:immunology*; D006801:Humans; D008264:Macrophages / Q000276:immunology; D008297:Male; D008810:Mice, Inbred C57BL; D050296:Microbial Viability / Q000187:drug effects; D012275:Oryza; D010587:Phagocytosis; D046549:Plantibodies / Q000276:immunology*; D016966:Porphyromonas gingivalis / Q000276:immunology / Q000502:physiology; D055815:Young Adult
chemical_list: D046549:Plantibodies; C063055:fimbrillin; D036781:Fimbriae Proteins