title: Efficacy of naringenin against permethrin-induced testicular toxicity in rats.
abstract: Permethrin (PM), a synthetic pyrethroid insecticide, has broad toxicity spectra. We aimed to investigate the effects of PM on the testes of adult albino rats, examine the recovery response and evaluate the efficacy of naringenin (NG) supplementation. Adult male albino rats were randomly assigned to five groups of six each: control, NG (50 mg/kg), PM (70 mg/kg), recovery (after subsequent withdrawal of PM) and NG-PM group. All treatments were given by oral gavage for 6 weeks and another 3 weeks for the recovery group. At the time of sacrifice, each testis was weighed. Biochemical analysis of epididymal sperm count and serum testosterone level was performed. Testes were processed for histological, ultrastructural and c-Kit immunohistochemical study. PM toxicity was evidenced by a highly significant decrease in testicular weight, epididymal sperm count and serum testosterone level compared to control. Furthermore, testicular structure abnormalities and reduced c-Kit immunoreactions were observed. Stoppage of PM in the recovery group partially reversed PM-induced changes. There was a mild decrease in testicular weight and biochemical parameters compared to control. The structure of seminiferous tubules was partially retained. The NG-PM group showed an overall improvement in testicular weight and biochemical alterations which were confirmed by light and electron microscopic examination. In conclusion, PM induced testicular toxicity, which was ameliorated by NG co-administration. However, stoppage of PM exposure was associated with partial recovery.
authors: Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Heba El-Sayed
authors: Hel-S
authors: Mostafa
authors: Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Samia A
authors: SA
authors: Abd El-Baset
authors: Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Asmaa A A
authors: http://orcid.org/0000-0002-7188-0100
authors: AA
authors: Kattaia
authors: Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Rania A
authors: RA
authors: Zidan
authors: Department of Community Medicine, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors: Mona M A
authors: MM
authors: Al Sadek
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004822:Epididymis / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009929:Organ Size / Q000187:drug effects*; D026023:Permethrin / Q000506:poisoning*; D051381:Rats; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013737:Testis / Q000187:drug effects*; D013739:Testosterone / Q000097:blood
chemical_list: D044950:Flavanones; D013739:Testosterone; D026023:Permethrin; C005273:naringenin
keywords: c-Kit; naringenin; permethrin; rats; testicular toxicity