title: Nicotinic modulation of Ca2+ oscillations in rat cortical neurons in vitro.
abstract: The roles of nicotine on Ca(2+) oscillations [intracellular Ca(2+) ([Ca(2+)]i) oscillation] in rat primary cultured cortical neurons were studied. The spontaneous [Ca(2+)]i oscillations (SCO) were recorded in a portion of the neurons (65%) cultured for 7-10 days in vitro. Application of nicotine enhanced [Ca(2+)]i oscillation frequency and amplitude, which were reduced by the selective α4β2-nicotinic acetylcholine receptors (nAChRs) antagonist dihydro-β-erythroidine (DHβE) hydrobromide, and the selective α7-nAChRs antagonist methyllycaconitine citrate (MLA, 20 nM). DHβE reduced SCO frequency and prevented the nicotinic increase in the frequency. DHβE somewhat enhanced SCO amplitude and prevented nicotinic increase in the amplitude. MLA (20 nM) itself reduced SCO frequency without affecting the amplitude but blocked nicotinic increase in [Ca(2+)]i oscillation frequency and amplitude. Furthermore, coadministration of both α4β2- and α7-nAChRs antagonists completely prevented nicotinic increment in [Ca(2+)]i oscillation frequency and amplitude. Thus, our results indicate that both α4β2- and α7-nAChRs mediated nicotine-induced [Ca(2+)]i oscillations, and two nAChR subtypes differentially regulated SCO.
authors: Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China; Department of Pathophysiology, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China;
authors: JianGang
authors: J
authors: Wang
authors: Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China; Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China; and.
authors: YaLi
authors: Y
authors: Wang
authors: Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China; Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China; and.
authors: FangLi
authors: F
authors: Guo
authors: Department of Anatomy, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China.
authors: ZhiBo
authors: Z
authors: Feng
authors: Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China;
authors: XiangFang
authors: X
authors: Wang
authors: Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China; Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan, Peoples Republic of China; and Johnlu9000@hotmail.com.
authors: ChengBiao
authors: C
authors: Lu
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009538:Nicotine / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000378:metabolism*
chemical_list: D011978:Receptors, Nicotinic; D009538:Nicotine; D002118:Calcium
keywords: calcium; intracellular calcium ion concentration oscillations; nicotine; α4β2-nicotinic acetylcholine receptors; α7-nicotinic acetylcholine receptors