title: Nuclear Translocation Sequence and Region in Autographa californica Multiple Nucleopolyhedrovirus ME53 That Are Important for Optimal Baculovirus Production.
abstract: Autographa californicamultiple nucleopolyhedrovirus (AcMNPV) is in the familyBaculoviridae, genusAlphabaculovirus AcMNPVme53is a highly conserved immediate early gene in all lepidopteran baculoviruses that have been sequenced and is transcribed up to late times postinfection. Althoughme53is not essential for viral DNA synthesis, infectious budded virus (BV) production is greatly attenuated when it is deleted. ME53 associates with the nucleocapsid on both budded virus and occlusion-derived virus, but not with the virus envelope. ME53 colocalizes in plasma membrane foci with the envelope glycoprotein GP64 in a GP64-dependent manner. ME53 localizes in the cytoplasm early postinfection, and despite the lack of a reported nuclear localization signal (NLS), ME53 translocates to the nucleus at late times postinfection. To map determinants of ME53 that facilitate its nuclear translocation, recombinant AcMNPV bacmids containing a series of ME53 truncations, internal deletions, and peptides fused with hemagglutinin (HA) or green fluorescent protein (GFP) tags were constructed. Intracellular-localization studies identified residues within amino acids 109 to 137 at the N terminus of ME53 that acted as the nuclear translocation sequence (NTS), facilitating its nuclear transport at late times postinfection. The first 100 N-terminal amino acids and the last 50 C-terminal amino acids of ME53 are dispensable for high levels of budded virus production. The region within amino acids 101 to 398, which also contains the NTS, is critical for optimal levels of budded virus production.
  
  
  OBJECTIVE
  Baculovirusme53is a conserved immediate early gene found in all sequenced lepidopteran alpha- and betabaculoviruses. We first identified residues within amino acids 109 to 137 at the N terminus that act as the ME53 nuclear translocation sequence (NTS) to facilitate its nuclear translocation and defined an internal region within amino acids 101 to 398, which includes the NTS, as being necessary for optimal budded virus production. Altogether, these results indicate a previously unidentified nuclear role that ME53 plays in virus replication.
authors: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
authors: Yang
authors: Y
authors: Liu
authors: Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada.
authors: Jondavid
authors: J
authors: de Jong
authors: Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada.
authors: Éva
authors: É
authors: Nagy
authors: Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada.
authors: David A
authors: DA
authors: Theilmann
authors: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada pkrell@uoguelph.ca.
authors: Peter J
authors: PJ
authors: Krell
mesh_terms: D000818:Animals; D002460:Cell Line; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli; D005814:Genes, Viral; D016297:Mutagenesis, Site-Directed; D019913:Nuclear Localization Signals; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D018411:Spodoptera / Q000821:virology; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: C079184:Ac-ME53 protein, Autographa californica nucleopolyhedrovirus; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D019913:Nuclear Localization Signals; D014764:Viral Proteins