title: Poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for specific recognition and release of lysozyme.
abstract: In this study, poly(amino acid)-based thermoresponsive molecularly imprinted magnetic nanoparticles for recognition and release of lysozyme was prepared via surface imprinting method. For constructing the molecularly imprinted polymer (MIP) layer, amino acid-based thermoresponsive monomer (N-methacryloyl-L-alanine methyl ester, MA-L-Ala-OMe) was mainly selected for the functional monomer along with N,N'-methylenebis(acrylamide) as the crosslinker. The resultant magnetic MIP nanoparticles were characterized in detail. Meanwhile, the dynamic light scattering studies and swelling ratios measurements were carried out for demonstrating the thermoresponsive property of the imprinted nanoparticles. The prepared magnetic MIP nanoparticles showed good adsorption capacity and selective recognition properties to lysozyme. Moreover, the fast adsorption process could reach equilibrium within 15 min. Importantly, the capture and release of lysozyme could be easily realized simply by altering the temperature of aqueous solution. Furthermore, the prepared imprinted nanoparticles were applied to separate lysozyme from the real egg white samples. The results proved that the thermoresponsive MIPs based on MA-L-Ala-OMe have great potential for selectively enriching target proteins in real samples.
authors: Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; Graduate School, Shandong Agricultural University, 271018 Shandong, PR China.
authors: Shilei
authors: S
authors: Ji
authors: Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; Graduate School, University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Nan
authors: N
authors: Li
authors: Beijing National Laboratory of Molecular Sciences, Lab of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; Graduate School, University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors: Yong
authors: Y
authors: Shen
authors: Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: qili@iccas.ac.cn.
authors: Qi
authors: Q
authors: Li
authors: Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors: Juan
authors: J
authors: Qiao
authors: Beijing National Laboratory of Molecular Sciences, Lab of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors: Zhibo
authors: Z
authors: Li
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D015394:Molecular Structure; D009113:Muramidase / Q000032:analysis* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013696:Temperature
chemical_list: D058185:Magnetite Nanoparticles; D011108:Polymers; D011109:Polymethacrylic Acids; C000611231:poly(N-methacryloylalanine methyl ester); D009113:Muramidase; D000409:Alanine
keywords: Lysozyme; Molecularly imprinted polymers (MIPs); Poly(amino acid); Surface imprinting magnetic particles; Thermoresponsive