title: Cytoskeletal architecture and cell motility remain unperturbed in mouse embryonic fibroblasts from Plk3 knockout mice.
abstract: Polo-like kinase 3 (Plk3) is best known for its involvement in cell cycle checkpoint regulation following exposure to cytotoxicants or induction of DNA damage. Yet, Plk3 has also been implicated in roles beyond those of cellular responses to DNA damage. Here, we have investigated the proposition, suggested by the Plk literature, that Plk3 regulates cytoskeletal architecture and cell functions mediated by the cytoskeleton. To this end, we have assayed mouse embryonic fibroblasts (MEFs) generated from both Plk3 knockout and wild-type mice. In particular, we asked whether Plk3 is involved in actin fiber and microtubule integrity, cell migration, cell attachment, and/or cell invasion. Our results demonstrate that functional Plk3 is not critical for the regulation of cytoskeletal integrity, cell morphology, cell adhesion, or motility in MEFs.
authors: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, OH 45267, USA.
authors: Daniel R
authors: DR
authors: Michel
authors: Department of Chemical and Materials Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.
authors: Kyu-Shik
authors: KS
authors: Mun
authors: Department of Chemical and Materials Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.
authors: Chia-Chi
authors: CC
authors: Ho
authors: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, OH 45267, USA peter.stambrook@uc.edu.
authors: Peter J
authors: PJ
authors: Stambrook
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D003599:Cytoskeleton / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D018345:Mice, Knockout; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency*
chemical_list: C093328:Plk3 protein, mouse; D017346:Protein Serine-Threonine Kinases
keywords: Polo-like kinase; and attachment; cell migration; cell motility; cytoskeleton; plk3