title: Differentiation of pluripotent stem cells for regenerative medicine.
abstract: A long-standing goal in regenerative medicine is to obtain scalable functional cells on demand to replenish cells lost in various conditions, including relevant diseases, injuries, and aging. As an unlimited cell source, pluripotent stem cells (PSCs) are invaluable for regenerative medicine, because they have the potential to give rise to any cell type in an organism. For therapeutic purposes, it is important to develop specific approach to directing PSC differentiation towards desired cell types efficiently. Through directed differentiation, PSCs could give rise to scalable, clinically relevant cells for in vivo transplantation, as well as for studying diseases in vitro and discovering drugs to treat them. Over the past few years, significant progress has been made in directing differentiation of PSCs into a variety of cell types. In this review, we discuss recent progress in directed differentiation of PSCs, clinical translation of PSC-based cell replacement therapies, and remaining challenges.
authors: Gladstone Institutes, San Francisco, CA 94158, USA; University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: ke.li@gladstone.ucsf.edu.
authors: Ke
authors: K
authors: Li
authors: Department of Biochemistry and Molecular Biology, School of Medicine, Southeast University, Nanjing, Jiangsu Province, 210009, China.
authors: Yan
authors: Y
authors: Kong
authors: Gladstone Institutes, San Francisco, CA 94158, USA; University of California, San Francisco, San Francisco, CA 94158, USA.
authors: Mingliang
authors: M
authors: Zhang
authors: Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
authors: Fei
authors: F
authors: Xie
authors: Gladstone Institutes, San Francisco, CA 94158, USA; University of California, San Francisco, San Francisco, CA 94158, USA.
authors: Peng
authors: P
authors: Liu
authors: Gladstone Institutes, San Francisco, CA 94158, USA; University of California, San Francisco, San Francisco, CA 94158, USA.
authors: Shaohua
authors: S
authors: Xu
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology*; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*
keywords: Cell therapy; Directed differentiation; Embryonic development; Pluripotent stem cells; Regenerative medicine; Small molecules