title: [Immunohistochemical changes in the lungs from fetuses and newborn infants at 20- 40 weeks' gestation with false left-sided congenital diaphragmatic hernia].
abstract: OBJECTIVE
  to study changes in the expression of growth factors in the lungs from fetuses and newborn infants at 20-40 weeks' gestation with false left-sided congenital diaphragmatic hernia (CDH).
  
  
  METHODS
  Lung fragments obtained at autopsies were examined using immunohistochemically stained paraffin sections. The expression indices of insulin-like growth factor-1 (IGF-1), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and transforming grow factor-β (TGF-β) in the fetal lung sections were compared with the results of survey and elective staining techniques, the morphometric data of digitized lung sections, and the indicators obtained in the comparison group.
  
  
  RESULTS
  Morphofunctional changes in the connective tissue, pulmonary vessels, and bronchial and alveolar epithelium at 20-24, 25-27, and 28-40 weeks' gestation were characterized, partly explaining the development of bilateral pulmonary hypoplasia in CDH. There was evidence for the involvement of PDGF, IGF-1, and EGF in primary pulmonary hypoplasia. PDGF deficiency plays a certain role in secondary pulmonary hypoplasia.
  
  
  CONCLUSIONS
  The findings can be used in researches and in the practical work of pathologists and forensic medical experts.
authors: Laboratory of Pathomorphology and Electron Microscopy, V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood, Ministry of Health of the Russian Federation, Ivanovo, Russia.
authors: L P
authors: LP
authors: Peretyatko
authors: Laboratory of Pathomorphology and Electron Microscopy, V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood, Ministry of Health of the Russian Federation, Ivanovo, Russia.
authors: E V
authors: EV
authors: Avrelkina
authors: Laboratory of Pathomorphology and Electron Microscopy, V.N. Gorodkov Ivanovo Research Institute of Maternity and Childhood, Ministry of Health of the Russian Federation, Ivanovo, Russia.
authors: A I
authors: AI
authors: Malyshkina
mesh_terms: D004815:Epidermal Growth Factor / Q000096:biosynthesis*; D004848:Epithelium / Q000473:pathology; D005333:Fetus / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D065630:Hernias, Diaphragmatic, Congenital / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
chemical_list: D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I