title: Inner membrane proteins YgdD and SbmA are required for the complete susceptibility ofEscherichia colito the proline-rich antimicrobial peptide arasin 1(1-25).
abstract: Arasin 1 from the spider crabHyas araneusis a proline-rich antimicrobial peptide (PR-AMP), which kills target bacteria by a non-membranolytic mechanism. By using a fluorescent derivative of the peptide, we showed that arasin 1 rapidly penetrates intoEscherichia colicells without membrane damage. To unravel its mode of action, a knockout gene library ofE. coliwas screened and two types of mutants with a less susceptible phenotype to the arasin 1 fragment (1-23) were found. The first bore the mutation ofsbmA, a gene coding for an inner membrane protein involved in the uptake of different antibiotic peptides. The second mutation was located in theygdDgene, coding for a conserved inner membrane protein of unknown function. Functional studies showed that YgdD is required for the full susceptibility to arasin 1(1-25), possibly by supporting its uptake and/or intracellular action. These results indicated that different bacterial proteins are exploited by arasin 1(1-25) to exert its antibacterial activity and add new insights on the complex mode of action of PR-AMPs.
authors: Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, 9037 Tromsø, Norway.
authors: Victoria S
authors: VS
authors: Paulsen
authors: Department of Life Sciences, University of Trieste, Via Giorgieri 5, 34127 Trieste, Italy.
authors: Mario
authors: M
authors: Mardirossian
authors: Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, 9037 Tromsø, Norway.
authors: Hans-Matti
authors: HM
authors: Blencke
authors: Department of Life Sciences, University of Trieste, Via Giorgieri 5, 34127 Trieste, Italy.
authors: Monica
authors: M
authors: Benincasa
authors: Department of Life Sciences, University of Trieste, Via Giorgieri 5, 34127 Trieste, Italy.
authors: Giulia
authors: G
authors: Runti
authors: Department of Life Sciences, University of Trieste, Via Giorgieri 5, 34127 Trieste, Italy.
authors: Matteo
authors: M
authors: Nepa
authors: Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, 9037 Tromsø, Norway.
authors: Tor
authors: T
authors: Haug
authors: Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT The Arctic University of Norway, 9037 Tromsø, Norway.
authors: Klara
authors: K
authors: Stensvåg
authors: Department of Life Sciences, University of Trieste, Via Giorgieri 5, 34127 Trieste, Italy.
authors: Marco
authors: M
authors: Scocchi