title: A Novel BLyS Peptibody Down-Regulates B Cell and T Helper Cell Subsets In Vivo and Ameliorates Collagen-Induced Arthritis.
abstract: B lymphocyte stimulator (BLyS), a member of tumor necrosis factor (TNF) family, contributes to the development of autoimmune disease, and BLyS antagonists have been developed for the treatment of autoimmune disorders. Recently, we constructed a novel BLyS antagonist, TC-Fc peptibody. The study was performed to investigate the efficiency of TC-Fc peptibody on collagen-induced arthritis (CIA). CIA mice were randomly divided into three groups, treated with TC-Fc, Fc, and phosphate-buffered saline (PBS), respectively. Clinical scores associated with the severity of arthritis were assessed on alternate day from first day. Histopathological scores, B and T cell changes, and autoantibodies levels were measured at the end of the experiment. CIA mice treated with TC-Fc peptibody had lower clinical and histological scores. Compared with Fc group, TC-Fc treatment resulted in reduction of B cell and T help cell subsets, significantly alleviated the swelling of paws, and suppressed articular tissue degeneration. These results demonstrated that TC-Fc could inhibit the progression of CIA and might have therapeutic effect on rheumatoid arthritis.
authors: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin, 300072, China.
authors: Weiwei
authors: W
authors: Zhu
authors: Department of Rheumatology & Immunology, Peking University People's Hospital and Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Beijing, 100044, China. sunxiaolin_sxl@126.com.|Department of Rheumatology & Immunology, Peking University Peoples' Hospital, No. 11 Xizhimen South Street, Xicheng District, Beijing, 100044, China. sunxiaolin_sxl@126.com.
authors: Xiaolin
authors: X
authors: Sun
authors: Department of Rheumatology & Immunology, Peking University People's Hospital and Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Beijing, 100044, China.
authors: Lei
authors: L
authors: Zhu
authors: Department of Rheumatology & Immunology, Peking University People's Hospital and Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Beijing, 100044, China.
authors: Yuzhou
authors: Y
authors: Gan
authors: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin, 300072, China.
authors: Rentuya
authors: R
authors: Baiwu
authors: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin, 300072, China.
authors: Jing
authors: J
authors: Wei
authors: Department of Rheumatology & Immunology, Peking University People's Hospital and Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Beijing, 100044, China.
authors: Zhanguo
authors: Z
authors: Li
authors: Department of Rheumatology & Immunology, Peking University People's Hospital and Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis (BZ0135), Beijing, 100044, China. doctorliru123@163.com.|Department of Rheumatology & Immunology, Peking University Peoples' Hospital, No. 11 Xizhimen South Street, Xicheng District, Beijing, 100044, China. doctorliru123@163.com.
authors: Ru
authors: R
authors: Li
authors: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin, 300072, China. sunj@tju.edu.cn.
authors: Jian
authors: J
authors: Sun
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy; D001323:Autoantibodies / Q000097:blood; D053264:B-Cell Activating Factor / Q000037:antagonists & inhibitors*; D001402:B-Lymphocytes / Q000276:immunology*; D003094:Collagen; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010455:Peptides / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D053264:B-Cell Activating Factor; D010455:Peptides; D003094:Collagen
keywords: BLyS; TC-Fc peptibody; antagonist; collagen-induced arthritis