title: Alteration of phospholipids in the blood of patients with Duchenne muscular dystrophy (DMD): in vitro, high resolution31P NMR-based study.
abstract: In vitro, high-resolution31P NMR (Nuclear Magnetic Resonance) spectroscopy-based analysis of phospholipids in serum is well recognized in leukemia, lymphoma, non-hematological cancers and renal cell carcinoma. In context of these studies, phospholipids were analyzed in blood of thirty-two (n = 32) patients with Duchenne muscular dystrophy (DMD) (Age, Mean ± SD; 8.0 ± 1.6 years) and sixteen (n = 16) healthy subjects (Age, Mean ± SD; 8.6 ± 2.3 years). Quantity of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS) and lyso-phosphatidylcholine (Lys-PC) was significantly higher (p < 0.05) in DMD patients as compared to healthy subjects. There were no significant differences (p > 0.05) observed for the quantity of phospholipids in blood of gene deletion positive cases of DMD as compared to negative gene deletion cases of DMD. Quantity of phospholipids in negative gene deletion cases of DMD patients as well as DMD cases with positive gene deletion was significantly higher (p < 0.05) as compared to normal individuals. The present study distinguishes the patients with DMD from the healthy subjects on the basis of the quantity of phospholipids in blood. These observations may be useful in future for the development of new diagnostic method of DMD.
authors: Department of Neurology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow, 226014, India. nirajsuprabhat@gmail.com.|School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. nirajsuprabhat@gmail.com.
authors: Niraj Kumar
authors: NK
authors: Srivastava
authors: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
authors: Somnath
authors: S
authors: Mukherjee
authors: Center of Biomedical Research, Sanjay Gandhi Postgraduate Institute of Medical Sciences Campus, Raebareli Road, Lucknow, 226014, India.
authors: Neeraj
authors: N
authors: Sinha
mesh_terms: D002648:Child; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D020388:Muscular Dystrophy, Duchenne / Q000097:blood*; D010743:Phospholipids / Q000097:blood*
chemical_list: D010743:Phospholipids
keywords: 31P NMR spectroscopy; Blood; DMD; Muscular dystrophy; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylinositol; Phosphatidylserine; Phospholipids