title: Molecular Cloning and Expression Determination of p38 MAPK from the Liver and Kidney of Silver Carp.
abstract: The sequence of p38MAPK in silver carp (Hm-p38a) was cloned and sequenced. Additionally, the acute toxicity of crude microcystins (MCs) on silver carp and induction expression of Hm-p38a by MCs exposure were also determined in this study. The results reveal that the length of Hm-p38a is 2418 bp and it contains a 1086 bp open reading frame. Hm-p38a could encode 361 amino acids. Sequence analysis indicates that Hm-p38a contains the conserved structures of Thr-Gly-Tyr motif and substrate binding site Ala-Thr-Arg-Trp, and it is highly conserved in fish. Phylogenetic analysis reveals that Hm-p38a is more closely related to fish than mammals and it belongs to p38α subfamily. Moreover, Hm-p38a in silver carp is constitutively expressed in all examined tissues. In addition, our results indicate that MCs exposure significantly promotes the transcription of Hm-p38a in fish liver or kidney, suggesting that mitogen-activated protein kinase should also be the signal pathway of MCs hepatotoxicity in fish.
authors: College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, People's Republic of China. lixiaoyu65@263.net.
authors: Xiaoyu
authors: X
authors: Li
authors: College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, People's Republic of China.
authors: Junguo
authors: J
authors: Ma
authors: College of Life Science, Henan Normal University, Xinxiang, Henan, 453007, People's Republic of China.
authors: Yuanyuan
authors: Y
authors: Li
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000633:toxicity*; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D029941:Fish Proteins / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D052998:Microcystins / Q000302:isolation & purification / Q000633:toxicity*; D046931:Microcystis / Q000737:chemistry / Q000472:pathogenicity; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014158:Transcription, Genetic; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D029941:Fish Proteins; D052998:Microcystins; C078588:microcystin; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Clone; Microcystins; Silver carp; Transcription; p38 MAPK