title: Surface lipoproteome of Mycoplasma hominis PG21 and differential expression after contact with human dendritic cells.
abstract: OBJECTIVE
  To assess the lipoproteins that are involved in the interaction between Mycoplasma hominis and human dendritic cells.
  
  
  METHODS
  The surface lipoproteome of M. hominis PG21 was characterized by using Triton X-114 extraction and LC-MS/MS identification. The transcriptional changes in lipoprotein genes upon contact with human dendritic cells were determined by using reverse transcription quantitative PCR after identification of reference genes suitable for normalization.
  
  
  RESULTS
  A large-scale overexpression of lipoprotein genes was observed with 21 upregulated transcripts. Seven genes of unknown function were M. hominis species specific and six genes were putatively associated with increased nutrient capture from the host cell and adhesion.
  
  
  CONCLUSIONS
  M. hominis regulates lipoprotein gene expression and may use species-specific mechanisms during the host colonization process.
authors: Université de Bordeaux, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|INRA, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|Laboratoire de Bactériologie, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France.
authors: Julien
authors: J
authors: Goret
authors: Université de Bordeaux, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|INRA, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.
authors: Chloé
authors: C
authors: Le Roy
authors: Université de Bordeaux, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|INRA, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.
authors: Arabella
authors: A
authors: Touati
authors: INSERM, U1047, Nîmes, France.
authors: Jennifer
authors: J
authors: Mesureur
authors: Université de Bordeaux, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|Laboratoire de Bactériologie, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France.
authors: Hélène
authors: H
authors: Renaudin
authors: Pôle Protéomique, Plateforme Génomique Fonctionnelle de Bordeaux, Université de Bordeaux, Bordeaux, France.
authors: Stéphane
authors: S
authors: Claverol
authors: Université de Bordeaux, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|INRA, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|Laboratoire de Bactériologie, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France.
authors: Cécile
authors: C
authors: Bébéar
authors: INRA, UMR 1332, Biologie du Fruit et Pathologie, Villenave d'Ornon, France.|Université de Bordeaux, UMR 1332, Biologie du Fruit et Pathologie, Bordeaux, France.
authors: Laure
authors: L
authors: Béven
authors: Université de Bordeaux, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|INRA, USC EA 3671 Mycoplasmal & Chlamydial Infections in Humans, Bordeaux, France.|Laboratoire de Bactériologie, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France.
authors: Sabine
authors: S
authors: Pereyre
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003198:Computer Simulation; D003713:Dendritic Cells / Q000382:microbiology*; D015870:Gene Expression; D005798:Genes, Bacterial; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D008074:Lipoproteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D019535:Mycoplasma hominis / Q000235:genetics* / Q000378:metabolism; D020543:Proteome; D060888:Real-Time Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D020543:Proteome
keywords: Mycoplasma hominis; RT-qPCR; dendritic cells; expression; lipoprotein; normalization; proteome; reference genes