title: Expression, purification and characterization of a thermostable leucine dehydrogenase from the halophilic thermophile Laceyella sacchari.
abstract: OBJECTIVE
  A potential thermotolerant L-leucine dehydrogenase from Laceyella sacchari (Ls-LeuDH) was over-expressed in E. coli, purified and characterized.
  
  
  RESULTS
  Ls-LeuDH had excellent thermostability with a specific activity of 183 U/mg at pH 10.5 and 25 °C. It retained a high activity in 200 mM carbonate buffer from pH 9.5 to 11. The optimal temperature for Ls-LeuDH was 60 °C.
  
  
  CONCLUSIONS
  It is the first time that a thermostable and highly active LeuDH originating from L. sacchari has been characterized. It may be useful for medical and pharmaceutical applications.
authors: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing, 211800, People's Republic of China.
authors: Wenjun
authors: W
authors: Zhu
authors: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing, 211800, People's Republic of China.
authors: Yan
authors: Y
authors: Li
authors: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing, 211800, People's Republic of China. hhjia@njtech.edu.cn.
authors: Honghua
authors: H
authors: Jia
authors: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing, 211800, People's Republic of China.
authors: Ping
authors: P
authors: Wei
authors: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing, 211800, People's Republic of China.
authors: Hua
authors: H
authors: Zhou
authors: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, Nanjing, 211800, People's Republic of China.
authors: Min
authors: M
authors: Jiang
mesh_terms: D000595:Amino Acid Sequence; D056449:Bacillales / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D050803:Leucine Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature
chemical_list: D011994:Recombinant Proteins; D050803:Leucine Dehydrogenase
keywords: Halophilic thermophile; Laceyella sacchari; Leucine dehydrogenase; Thermostable dehydrogenase