title: Caspofungin-induced in-vitro post-antifungal effect and its impact on adhesion related traits of oral Candida dubliniensis and Candida albicans isolates.
abstract: Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulence traits involved in the pathogenicity of Candida. Post-antifungal effect (PAFE) also have a bearing on pathogenicity and virulence of Candida. Candida dubliniensis is associated with oral and systemic candidosis, which can be managed with caspofungin. There is no published information on caspofungin-induced PAFE and its impact on adhesion traits of C. dubliniensis isolates. Thus, the purpose of this investigation was to determine the in vitro duration of PAFE on 20 C. dubliniensis isolates following transient exposure to caspofungin. Furthermore the impacts of caspofungin-induced PAFE on adhesion to BEC and DAS, GT formation and CSH of these isolates were also determined. After establishing the minimum inhibitory concentration (MIC) of caspofungin, C. dubliniensis isolates were exposed to sub-lethal concentrations (×3 MIC) of caspofungin for 1 hr. Thereafter the duration of PAFE, adhesion to BEC and DAS, GT formation and CSH were determined by previously described in-vitro assays. MIC (μg/mL) of C. dubliniensis isolates to caspofungin ranged from 0.004 to 0.19. Caspofungin-induced mean PAFE on C. dubliniensis isolates was 2.17 hr. Exposure to caspofungin suppressed the ability of C. dubliniensis isolates to adhere to BEC and DAS, form GT and CSH by 69.97%, 71.95%, 90.06% and 32.29% (P < 0.001 for all), respectively. Thus, transient exposure of C. dubliniensis isolates to caspofungin produces an antifungal effect not only by suppressing its growth but also by altering its adhesion traits.
authors: Department of Bioclinical Sciences, Faculty of Dentistry.
authors: Arjuna Nishantha Bandara
authors: AN
authors: Ellepola
authors: Department of Microbiology, Faculty of Medicine, Health Sciences Center, Kuwait University, Kuwait.
authors: Rachel
authors: R
authors: Chandy
authors: Department of Microbiology, Faculty of Medicine, Health Sciences Center, Kuwait University, Kuwait.
authors: Zia Uddin
authors: ZU
authors: Khan
authors: School of Dentistry, University of Queensland, Brisbane, Australia.
authors: Lakshman Perera
authors: LP
authors: Samaranayake
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000166:cytology / Q000187:drug effects* / Q000302:isolation & purification; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects* / Q000302:isolation & purification; D002177:Candidiasis / Q000188:drug therapy; D002180:Candidiasis, Oral / Q000382:microbiology; D000077336:Caspofungin; D002448:Cell Adhesion / Q000187:drug effects*; D003819:Denture Bases / Q000382:microbiology; D054714:Echinocandins / Q000494:pharmacology*; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055666:Lipopeptides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D000077336:Caspofungin
keywords: Adhesion; Candida albicans; Candida dubliniensis; caspofungin; post-antifungal effect