title: Manganese elevates manganese superoxide dismutase protein level through protein kinase C and protein tyrosine kinase.
abstract: Three experiments were conducted to investigate the effects of inorganic and organic Mn sources on MnSOD mRNA, protein and enzymatic activity and the possible signal pathways. The primary broiler myocardial cells were treated with MnCl2 (I) or one of organic chelates of Mn and amino acids with weak, moderate (M) or strong (S) chelation strength for 12 and 48 h. Cells were preincubated with superoxide radical anions scavenger N-acetylcysteine (NAC) or specific inhibitors for MAPKs and protein tyrosine kinase (PTK) or protein kinase C (PKC) for 30 min before treatments of I and M. The MnSOD mRNA, protein and enzymatic activity, phosphorylated MAPKs or protein kinases activations were examined. The results showed that additions of Mn increased (P < 0.05) MnSOD mRNA levels and M was more effective than I. Additions of Mn elevated (P < 0.05) MnSOD protein levels and enzymatic activities, and no differences were found among I and M. Addition of NAC did not decrease (P > 0.05) Mn-induced MnSOD mRNA and protein levels. None of the three MAPKs was phosphorylated (P > 0.05) by Mn. Additions of Mn decreased (P < 0.05) the PTK activities and increased (P < 0.05) the membrane PKC contents. Inhibitors for PTK or PKC decreased (P < 0.05) Mn-induced MnSOD protein levels. The results suggested that Mn-induced MnSOD mRNA and protein expressions be not related with NAC, and MAPK pathways might not involve in Mn-induced MnSOD mRNA expression. PKC and PTK mediated the Mn-induced MnSOD protein expression.
authors: Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.|Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, People's Republic of China.
authors: Sufen
authors: S
authors: Li
authors: Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.
authors: Lin
authors: L
authors: Lu
authors: Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.
authors: Xiudong
authors: X
authors: Liao
authors: Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.
authors: Tianquan
authors: T
authors: Gao
authors: Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.
authors: Funing
authors: F
authors: Wang
authors: Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China.
authors: Liyang
authors: L
authors: Zhang
authors: Department of Animal Science, North Carolina State University, Raleigh, NC, 27695, USA.
authors: Lin
authors: L
authors: Xi
authors: Key Laboratory of Animal Nutrition and Feed Science of the Ministry of Agriculture, Guangdong Wen's Foodstuffs Group Corporation Ltd., Yunfu, 527400, People's Republic of China.
authors: Songbai
authors: S
authors: Liu
authors: Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan West Road, Haidian, Beijing, 100193, People's Republic of China. wlysz@263.net.
authors: Xugang
authors: X
authors: Luo
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D004789:Enzyme Activation; D008297:Male; D008345:Manganese / Q000494:pharmacology*; D010766:Phosphorylation; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D008345:Manganese; D013482:Superoxide Dismutase; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C
keywords: Manganese; Manganese superoxide dismutase; Mitogen-activated protein kinases; Primary broiler myocardial cell; Protein kinase C; Protein tyrosine kinase