title: [THE EFFECT OF waaL LIGASE GENES DELETION ON MOTILITY AND STRESS ADAPTATION REACTIONS OF YERSINIA ENTEROCOLITICA 6471/76].
abstract: The aim of current study was to estimate the influence of waaL(OS) and waaL(PS) genes deletion on lipopolysaccharide (LPS) synthesis, bacterial motility and stress resistance of bacteria Yersinia enterocolitica 6471/76. Single and double waaL mutants were created by replacing the wild-type alleles in bacterial chromosome for mutant ones. The phenotypes of mutants were visualized by DOC-PAGE gels stained with silver and immunoblot with specific to O-polysaccharide and outer core monoclonal antibodies. Bacterial motility was evaluated by the diameter of the migration zone. Wild type bacteria and mutants were analyzed by bacterial growth curves in a hypertonic medium. Participation of WaaL ligases in resistance to osmotic pressure was found only in case of both ligese genes deletion. Also the YeO3_os_ps mutants showed motility decreasing, which recovered after adding a functionally active gene. Thus, deletion of both waaL ligase genes lead to a drastic reduction in bacterial motility and increase their sensitivity to hypertonic medium that can indirectly characterize biological role of WaaL ligases.
authors: J I
authors: JI
authors: Shevchenko
authors: J V
authors: JV
authors: Shilina
authors: V K
authors: VK
authors: Pozur
authors: M
authors: M
authors: Skurnik
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D018407:Bacterial Physiological Phenomena / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000235:genetics*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D009154:Mutation; D009997:Osmotic Pressure / Q000502:physiology; D013312:Stress, Physiological / Q000235:genetics*; D015008:Yersinia enterocolitica / Q000201:enzymology* / Q000235:genetics / Q000502:physiology
chemical_list: D008070:Lipopolysaccharides; D019729:Carbon-Oxygen Ligases