title: Podosome Formation and Development in Monocytes Restricted by the Nanoscale Spatial Distribution of ICAM1.
abstract: We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.
authors: Interdisciplinary Nanoscience Center (iNANO), Århus University , Århus 8000, Denmark.
authors: Andreas S
authors: AS
authors: Andersen
authors: Interdisciplinary Nanoscience Center (iNANO), Århus University , Århus 8000, Denmark.
authors: Hüsnü
authors: H
authors: Aslan
authors: Interdisciplinary Nanoscience Center (iNANO), Århus University , Århus 8000, Denmark.
authors: Mingdong
authors: M
authors: Dong
authors: National Center for Nanoscience and Technology (NCNST), Chinese Academy of Sciences (CAS) , Beijing, China.
authors: Xingyu
authors: X
authors: Jiang
authors: Interdisciplinary Nanoscience Center (iNANO), Århus University , Århus 8000, Denmark.
authors: Duncan S
authors: DS
authors: Sutherland
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D006801:Humans; D055767:Immobilized Proteins / Q000032:analysis / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D000069261:Podosomes / Q000378:metabolism*
chemical_list: D055767:Immobilized Proteins; D018799:Intercellular Adhesion Molecule-1
keywords: ICAM1; THP1; adhesion; nanopattern; podosome