title: Antimicrobial resistance dashboard application for mapping environmental occurrence and resistant pathogens.
abstract: An antibiotic resistance (AR) Dashboard application is being developed regarding the occurrence of antibiotic resistance genes (ARG) and bacteria (ARB) in environmental and clinical settings. The application gathers and geospatially maps AR studies, reported occurrence and antibiograms, which can be downloaded for offline analysis. With the integration of multiple data sets, the database can be used on a regional or global scale to identify hot spots for ARGs and ARB; track and link spread and transmission, quantify environmental or human factors influencing presence and persistence of ARG harboring organisms; differentiate natural ARGs from those distributed via human or animal activity; cluster and compare ARGs connections in different environments and hosts; and identify genes that can be used as proxies to routinely monitor anthropogenic pollution. To initially populate and develop the AR Dashboard, a qPCR ARG array was tested with 30 surface waters, primary influent from three waste water treatment facilities, ten clinical isolates from a regional hospital and data from previously published studies including river, park soil and swine farm samples. Interested users are invited to download a beta version (available on iOS or Android), submit AR information using the application, and provide feedback on current and prospective functionalities.
authors: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors: Robert D
authors: RD
authors: Stedtfeld
authors: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors: Maggie R
authors: MR
authors: Williams
authors: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors: Umama
authors: U
authors: Fakher
authors: The Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.
authors: Timothy A
authors: TA
authors: Johnson
authors: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors: Tiffany M
authors: TM
authors: Stedtfeld
authors: The Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
authors: Fang
authors: F
authors: Wang
authors: Department of Microbiology, Sparrow Laboratories, Sparrow Health System, Lansing, MI 48912, USA.
authors: Walid T
authors: WT
authors: Khalife
authors: Department of Osteopathic Medical Specialties, Section of Emergency Medicine, College of Osteopathic Medicine, Michigan State University, East Lansing, MI 48824, USA.
authors: Mary
authors: M
authors: Hughes
authors: Department of Osteopathic Medical Specialties, Section of Emergency Medicine, College of Osteopathic Medicine, Michigan State University, East Lansing, MI 48824, USA.
authors: Brett E
authors: BE
authors: Etchebarne
authors: The Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
authors: James M
authors: JM
authors: Tiedje
authors: Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA The Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA hashsham@egr.msu.edu.
authors: Syed A
authors: SA
authors: Hashsham
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D001426:Bacterial Proteins / Q000235:genetics; D016208:Databases, Factual; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D011446:Prospective Studies; D045483:Rivers / Q000382:microbiology; D013552:Swine / Q000382:microbiology; D062065:Wastewater / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D062065:Wastewater
keywords: AR dashboard database application; antibiotic resistance gene qPCR array; environmental resistome; mapping genes; mobile genetic elements