title: Identification of avian leukosis virus subgroup J-associated acutely transforming viruses carrying the v-src oncogene in layer chickens.
abstract: To elucidate the molecular basis for the rapid oncogenicity of an acutely transforming avian leukosis virus (ALV), isolated from fibrosarcomas in Hy-Line Brown commercial layer chickens infected with ALV subgroup J (ALV-J), the complete genomic structure of the provirus was determined. In addition to ALV-J replication-complete virus SDAU1102, five proviral DNA genomes, named SJ-1, SJ-2, SJ-3, SJ-4 and SJ-5, carrying different lengths of the v-src oncogene were amplified from original tumours and chicken embryo fibroblasts (CEFs) infected with viral stocks. The genomic sequences of the SJ-1-SJ-5 provirus were closely related to that of SDAU1102 but were defective. The results of Western blot analysis and immunohistochemical staining also showed overexpression of the p60v-src protein in infected CEFs and tumour tissue. To the best of our knowledge, this is the first report of the isolation and identification of acutely transforming viruses carrying the v-src oncogene with ALV-J as the helper virus. It also offers insight into the generation of acutely transforming ALVs carrying the v-src oncogene.
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Yixin
authors: Y
authors: Wang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Jianliang
authors: J
authors: Li
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Yang
authors: Y
authors: Li
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Lichun
authors: L
authors: Fang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Xiaolong
authors: X
authors: Sun
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Shuang
authors: S
authors: Chang
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Peng
authors: P
authors: Zhao
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University,Daizong Road No. 61, Tai'an, Shandong, 271018, PRChina.
authors: Zhizhong
authors: Z
authors: Cui
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000175:diagnosis / Q000821:virology*; D001354:Avian Leukosis Virus / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D004279:DNA, Viral; D016391:Genes, src; D016679:Genome, Viral
chemical_list: D004279:DNA, Viral