title: Effects of aluminum chloride-catalyzed hydrothermal pretreatment on the structural characteristics of lignin and enzymatic hydrolysis.
abstract: In this study, Eucalyptus camaldulensis was pretreated with 0.02 M aluminum chloride (AlCl3) at 140-180 °C to obtain digestible substrates for glucose and lignin. The effects of AlCl3-catalyzed hydrothermal pretreatment on the degradation of carbohydrates, structural changes of lignin, crystallinity, morphologic changes, and cellulose conversion of the pretreated biomass have been investigated by HAPEC, HPLC, FT-IR, XRD, CP/MAS NMR, SEM, and 2D-HSQC NMR. Results showed that the pretreatment significantly removed hemicelluloses and cleaved β-O-4 linkages of lignin at high temperatures. Under an optimum condition (at 170 °C for 1 h), almost all of hemicelluloses were removed and most of β-O-4 linkages in lignin were cleaved, and 77.8% cellulose conversion of the pretreated biomass was achieved, which was 7.3-fold higher than that of the original biomass. In short, this process was regarded as a promising approach to achieve an efficient conversion of lignocellulosic biomass to fermentable glucose and residual lignin.
authors: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors: Xiao-Jun
authors: XJ
authors: Shen
authors: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors: Bing
authors: B
authors: Wang
authors: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors: Pan-Li
authors: PL
authors: Huang
authors: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China. Electronic address: wenjialong@bjfu.edu.cn.
authors: Jia-Long
authors: JL
authors: Wen
authors: Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
authors: Run-Cang
authors: RC
authors: Sun
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000737:chemistry*; D018533:Biomass; D002241:Carbohydrates; D002384:Catalysis; D002482:Cellulose / Q000378:metabolism; D002712:Chlorides / Q000737:chemistry*; D005052:Eucalyptus / Q000737:chemistry*; D005285:Fermentation; D006358:Hot Temperature; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D017607:Aluminum Compounds; D002241:Carbohydrates; D002712:Chlorides; D011134:Polysaccharides; D000077410:Aluminum Chloride; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin
keywords: AlCl(3)-catalyzed hydrothermal pretreatment; Enzymatic saccharification; Eucalyptus camaldulensis; Lignin; Structural features