title: Structural basis for cellulose binding by the type A carbohydrate-binding module 64 of Spirochaeta thermophila.
abstract: Spirochaeta thermophila secretes seven glycoside hydrolases for plant biomass degradation that carry a carbohydrate-binding module 64 (CBM64) appended at the C-terminus. CBM64 adsorbs to various β1-4-linked pyranose substrates and shows high affinity for cellulose. We present the first crystal structure of a CBM64 at 1.2 Å resolution, which reveals a jelly-roll-like fold corresponding to a surface-binding type A CBM. Modeling of its interaction with cellulose indicates that CBM64 achieves association with the hydrophobic face of β-linked pyranose chains via a unique coplanar arrangement of four exposed tryptophan side chains. Proteins 2016; 84:855-858. © 2016 Wiley Periodicals, Inc.
authors: Munich Center for Integrated Protein Science (CIPS-M) and Lehrstuhl für Biologische Chemie, Technische Universität München, 85354 Freising (Weihenstephan), Germany.
authors: André
authors: A
authors: Schiefner
authors: Lehrstuhl Für Mikrobiologie, Technische Universität München, 85354 Freising (Weihenstephan), Germany.
authors: Angel
authors: A
authors: Angelov
authors: Lehrstuhl Für Mikrobiologie, Technische Universität München, 85354 Freising (Weihenstephan), Germany.
authors: Wolfgang
authors: W
authors: Liebl
authors: Munich Center for Integrated Protein Science (CIPS-M) and Lehrstuhl für Biologische Chemie, Technische Universität München, 85354 Freising (Weihenstephan), Germany.
authors: Arne
authors: A
authors: Skerra
mesh_terms: D001665:Binding Sites; D002482:Cellulose / Q000378:metabolism*; D018360:Crystallography, X-Ray; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D013142:Spirochaeta / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism
chemical_list: D002482:Cellulose; D006026:Glycoside Hydrolases
keywords: CBM64; carbohydrate-binding module; cellulose-binding domain; type A CBM; β-sandwich