title: The characteristics of genetically related Pseudomonas aeruginosa from diverse sources and their interaction with human cell lines.
abstract: We investigated a collection of Pseudomonas aeruginosa strains from hospitalised patients (n = 20) and various environmental sources (n = 214) for their genetic relatedness; virulence properties; antibiotic resistance; and interaction with intestinal (Caco-2), renal (A-498), and lung (Calu-3) cell lines. Using RAPD-PCR, we found high diversity among the strains irrespective of their sources, with only 6 common (C) types containing strains from both a clinical and environmental source. Environmental strains belonging to these C-types showed greater adhesion to A-498 cells than did clinical strains (17 ± 13 bacteria/cell versus 13 ± 11 bacteria/cell; p < 0.001), whereas clinical strains showed significantly greater adhesion to Calu-3 and Caco-2 cells than did environmental strains (p < 0.001 for both). The virulence genes and antibiotic resistance profiles of the strains were similar; however, the prevalence of environmental strains carrying both exoS and exoU was significantly (p < 0.0368) higher than clinical strains. While all strains were resistant to ticarcillin and ticarcillin-clavulanic acid, resistance against aztreonam, gentamicin, amikacin, piperacillin, and ceftazidime varied among environmental and clinical strains. These results suggest that environmental strains of P. aeruginosa carry virulence properties similar to clinical strains, including adhesion to various human cell lines, with some strains showing a higher adhesion to specific cell lines, indicating they may have a better ability to cause infection in those sites under predisposing conditions of the host.
authors: Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.|Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.
authors: Klrissa
authors: K
authors: Streeter
authors: Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.|Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.
authors: Christina
authors: C
authors: Neuman
authors: Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.|Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.
authors: Jasmin
authors: J
authors: Thompson
authors: Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.|Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.
authors: Eva
authors: E
authors: Hatje
authors: Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.|Genecology Research Centre, School of Health and Sport Sciences, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, 4558, Queensland, Australia.
authors: Mohammad
authors: M
authors: Katouli
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018938:Caco-2 Cells; D002969:Clavulanic Acids / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000187:drug effects; D004783:Environmental Microbiology; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D019105:Random Amplified Polymorphic DNA Technique; D013982:Ticarcillin / Q000494:pharmacology; D014774:Virulence / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; C043215:ticarcillin-clavulanic acid; D013982:Ticarcillin
keywords: Pseudomonas aeruginosa; adhesion; adhérence; antibiotic resistance; résistance aux antibiotiques; virulence