title: An Electrochemical Impedance Spectroscopy System for Monitoring Pineapple Waste Saccharification.
abstract: Electrochemical impedance spectroscopy (EIS) has been used for monitoring the enzymatic pineapple waste hydrolysis process. The system employed consists of a device called Advanced Voltammetry, Impedance Spectroscopy &amp; Potentiometry Analyzer (AVISPA) equipped with a specific software application and a stainless steel double needle electrode. EIS measurements were conducted at different saccharification time intervals: 0, 0.75, 1.5, 6, 12 and 24 h. Partial least squares (PLS) were used to model the relationship between the EIS measurements and the sugar determination by HPAEC-PAD. On the other hand, artificial neural networks: (multilayer feed forward architecture with quick propagation training algorithm and logistic-type transfer functions) gave the best results as predictive models for glucose, fructose, sucrose and total sugars. Coefficients of determination (R²) and root mean square errors of prediction (RMSEP) were determined as R² > 0.944 and RMSEP < 1.782 for PLS and R² > 0.973 and RMSEP < 0.486 for artificial neural networks (ANNs), respectively. Therefore, a combination of both an EIS-based technique and ANN models is suggested as a promising alternative to the traditional laboratory techniques for monitoring the pineapple waste saccharification step.
authors: Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain. clcodo@upvnet.upv.es.
authors: Claudia
authors: C
authors: Conesa
authors: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València-Universitat de València, Camí de Vera s/n, 46022 Valencia, Spain. jibanyez@eln.upv.es.
authors: Javier
authors: J
authors: Ibáñez Civera
authors: Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain. lusegil@upvnet.upv.es.
authors: Lucía
authors: L
authors: Seguí
authors: Instituto de Ingeniería de Alimentos para el Desarrollo (IIAD), Universitat Politècnica de València, Camí de Vera s/n, 46022 Valencia, Spain. pfito@tal.upv.es.
authors: Pedro
authors: P
authors: Fito
authors: Centro de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Unidad Mixta Universitat Politècnica de València-Universitat de València, Camí de Vera s/n, 46022 Valencia, Spain. nilami@iqn.upv.es.
authors: Nicolás
authors: N
authors: Laguarda-Miró
mesh_terms: D000465:Algorithms; D031230:Ananas / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D005632:Fructose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D012037:Refuse Disposal; D013395:Sucrose / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D005632:Fructose; D013395:Sucrose; D005947:Glucose
keywords: electrochemical impedance spectroscopy; monitoring; pineapple waste; saccharification