title: Lophirones B and C prevent aflatoxin B1-induced oxidative stress and DNA fragmentation in rat hepatocytes.
abstract: Context Despite the reported anticarcinogenic activity of lophirones B and C, no scientific information exists for its activity in rat hepatocytes. Objective Effect of lophirones B and C on aflatoxin B1 (AFB1)-induced oxidative stress, and DNA fragmentation in rat hepatocytes was investigated. Materials and methods Wistar rat hepatocytes were incubated with lophirones B and C (1 mg/mL) or sylimarin (1 mg/mL) in the presence or absence of AFB1. For an in vivo study, rats were orally administered with lophirones B and C, and/or AFB1 (20 μg/d) for 9 weeks. Results Lophirones B and C lowered AFB1-mediated increase in nitric oxide, superoxide anion radicals, caspase-3 and fragmented DNA. Lophirones B and C attenuated AFB1-mediated decrease in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and reduced glutathione. Also, lophirones B and C attenuated AFB1-mediated increase in conjugated dienes, lipid hydroperoxides and malondialdehyde in rat hepatocytes. Furthermore, AFB1-mediated alterations in alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, total bilirubin and globulin in rat serum were significantly annulled in lophirones B and C-treated rats. Conclusion This study revealed that lophirones B and C prevented AFB1-induced oxidative damage in rat hepatocytes.
authors: a Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences , Al-Hikmah University , Ilorin , Nigeria ;
authors: Taofeek Olakunle
authors: TO
authors: Ajiboye
authors: b Phytomedicine, Toxicology and Reproductive Research Laboratory, Department of Biochemistry , University of Ilorin , Ilorin , Nigeria.
authors: Musa Toyin
authors: MT
authors: Yakubu
authors: b Phytomedicine, Toxicology and Reproductive Research Laboratory, Department of Biochemistry , University of Ilorin , Ilorin , Nigeria.
authors: Adenike Temidayo
authors: AT
authors: Oladiji
mesh_terms: D000284:Administration, Oral; D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D047188:Chalcones / Q000008:administration & dosage / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D019610:Cytoprotection; D053938:DNA Fragmentation / Q000187:drug effects*; D004334:Drug Administration Schedule; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008111:Liver Function Tests; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012838:Silymarin / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D047188:Chalcones; D012838:Silymarin; C000613963:lophirone B; C000613964:lophirone C; D016604:Aflatoxin B1
keywords: Caspase-3; chalcone dimmers; fragmented DNA; lipid peroxidation; oxidative stress biomarkers