title: Do biomarkers of exposure and effect correlate with internal exposure to PAHs in swine?
abstract: Humans are commonly exposed to polycyclic aromatic hydrocarbons (PAHs), a family of compounds present as mixtures in the environment. This study exposed swine to PAH mixtures in single and subacute dose regimens and collected liver and ileum tissue to measure cytochrome P450 mRNA expression and enzyme activity as biomarkers of exposure and DNA adducts and oxidized proteins as biomarkers of effect. Micronucleated reticulocytes were measured as systemic biomarkers of effect. Duration of exposure did not influence biomarkers of exposure, though exposure duration produced significant increases in DNA adducts and oxidative stress. Micronucleated reticulocyte numbers were not affected by exposure length.
authors: a Department of Soil Science , University of Saskatchewan , Saskatoon , Canada .|b Toxicology Graduate Program, University of Saskatchewan , Saskatoon , Canada , and.
authors: Rachel E
authors: RE
authors: Peters
authors: c Department of Veterinary Biomedical Sciences , University of Saskatchewan , Saskatoon , Canada.
authors: Mark
authors: M
authors: Wickstrom
authors: a Department of Soil Science , University of Saskatchewan , Saskatoon , Canada .
authors: Steven D
authors: SD
authors: Siciliano
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis* / Q000235:genetics; D019388:Cytochrome P-450 CYP1A2 / Q000096:biosynthesis* / Q000235:genetics; D018736:DNA Adducts / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012156:Reticulocytes / Q000187:drug effects; D013552:Swine
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D012333:RNA, Messenger; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2
keywords: BPDE adducts; EROD; Pig CYPs; micronuclei; oxidative stress