title: Capillary electrophoresis combined in-line with solid-phase extraction using magnetic particles as new adsorbents for the determination of drugs of abuse in human urine.
abstract: A simple approach is presented based on the in-line coupling between magnetic particles-based SPE and CE. Silica-coated iron oxide particles functionalized with C18 were successfully synthesized and used as a reverse-phase sorbent for in-line SPE-CE. Magnets were used to locally immobilize these sorbents inside the capillary. Four drugs of abuse were preconcentrated and determined in urine samples using the developed method with a simple pretreatment procedure based on LLE. Several parameters affecting the preconcentration were evaluated. The obtained results show that this strategy enhanced detection sensitivity in the range of 125-700-fold compared with CE without preconcentration. The developed method provides LODs (S/N = 3) for standard samples in the range of 0.5-20 ng/mL with satisfactory analytical precision, in both intraday and day-to-day experiments (RSDs <20%). The LODs (S/N = 3) reached for urine samples were in the range of 20-50 ng/mL. Relative recoveries greater than 75.9% were obtained. The established method has been applied to the analysis of drugs of abuse in urine samples from drug abusers.
authors: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Tarragona, Spain.
authors: Tatiana
authors: T
authors: Baciu
authors: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Tarragona, Spain.
authors: Francesc
authors: F
authors: Borrull
authors: Department of Chemistry, Aalen University, Aalen, Germany.
authors: Christian
authors: C
authors: Neusüß
authors: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Tarragona, Spain.
authors: Carme
authors: C
authors: Aguilar
authors: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Tarragona, Spain.
authors: Marta
authors: M
authors: Calull
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000302:isolation & purification / Q000652:urine*; D057230:Limit of Detection; D016014:Linear Models; D058185:Magnetite Nanoparticles; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D013287:Illicit Drugs; D058185:Magnetite Nanoparticles
keywords: Bioanalysis; Capillary electrophoresis; Drugs of abuse; In-line solid-phase extraction; Magnetic particles