title: Click Reaction on Solid Phase Enables High Fidelity Synthesis of Nucleobase-Modified DNA.
abstract: The post-synthetic functionalization of nucleic acids via click chemistry (CuAAC) has seen tremendous implementation, extending the applicability of nucleobase-modified nucleic acids in fields like fluorescent labeling, nanotechnology, and in vitro selection. However, the production of large quantities of high-density functionalized material via solid phase synthesis has been hampered by oxidative by-product formation associated with the alkaline workup conditions. Herein, we describe a rapid and cost-effective protocol for the high fidelity large-scale production of nucleobase-modified nucleic acids, exemplified with a recently described nucleobase-modified aptamer.
authors: Life and Medical Sciences Institute, University of Bonn , Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
authors: Fabian
authors: F
authors: Tolle
authors: Life and Medical Sciences Institute, University of Bonn , Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
authors: Malte
authors: M
authors: Rosenthal
authors: Life and Medical Sciences Institute, University of Bonn , Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
authors: Franziska
authors: F
authors: Pfeiffer
authors: Life and Medical Sciences Institute, University of Bonn , Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
authors: Günter
authors: G
authors: Mayer
mesh_terms: D002851:Chromatography, High Pressure Liquid; D057930:Click Chemistry; D004247:DNA / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D009711:Nucleotides; D004247:DNA