title: Carboxyl- and amino-functionalized polystyrene nanoparticles differentially affect the polarization profile of M1 and M2 macrophage subsets.
abstract: Macrophages are key regulators of innate and adaptive immune responses. Exposure to microenvironmental stimuli determines their polarization into proinflammatory M1 and anti-inflammatory M2 macrophages. M1 exhibit high expression of proinflammatory TNF-α and IL-1β, and M2 promote tissue repair, but likewise support tumor growth and cause immune suppression by expressing IL-10. Thus, the M1/M2 balance critically determines tissue homeostasis. By using carboxyl- (PS-COOH) and amino-functionalized (PS-NH2) polystyrene nanoparticles, the effects of surface decoration on the polarization of human macrophages were investigated. The nanoparticles did not compromise macrophage viability nor did they affect the expression of the M1 markers CD86, NOS2, TNF-α, and IL-1β. By contrast, in M2, both nanoparticles impaired expression of scavenger receptor CD163 and CD200R, and the release of IL-10. PS-NH2 also inhibited phagocytosis of Escherichia coli by both, M1 and M2. PS-COOH did not impair phagocytosis by M2, but increased protein mass in M1 and M2, TGF-β1 release by M1, and ATP levels in M2. Thus, nanoparticles skew the M2 macrophage polarization without affecting M1 markers. Given the critical role of the M1 and M2 polarization for the immunological balance in patients with cancer or chronic inflammation, functionalized nanoparticles might serve as tools for reprogramming the M1/M2 polarization.
authors: Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, D-89081 Ulm, Germany.
authors: Ann-Kathrin
authors: AK
authors: Fuchs
authors: Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, D-89081 Ulm, Germany.
authors: Tatiana
authors: T
authors: Syrovets
authors: Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, D-89081 Ulm, Germany.
authors: Karina A
authors: KA
authors: Haas
authors: Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, D-89081 Ulm, Germany.
authors: Cornelia
authors: C
authors: Loos
authors: Max Planck Institute for Polymer Research, D-55128 Mainz, Germany; Fraunhofer ICT-IMM, D-55129 Mainz, Germany.
authors: Anna
authors: A
authors: Musyanovych
authors: Max Planck Institute for Polymer Research, D-55128 Mainz, Germany; Department of Dermatology, University Medical Center Mainz, D-55131 Mainz, Germany.
authors: Volker
authors: V
authors: Mailänder
authors: Max Planck Institute for Polymer Research, D-55128 Mainz, Germany.
authors: Katharina
authors: K
authors: Landfester
authors: Institute of Pharmacology of Natural Products & Clinical Pharmacology, Ulm University, D-89081 Ulm, Germany. Electronic address: thomas.simmet@uni-ulm.de.
authors: Thomas
authors: T
authors: Simmet
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D064446:Orexin Receptors; D010587:Phagocytosis / Q000187:drug effects; D011137:Polystyrenes / Q000737:chemistry / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; D051940:B7-2 Antigen; D001672:Biocompatible Materials; D000098902:CD163 Antigen; C477337:CD200R1 protein, human; C508609:IL10 protein, human; C508585:IL1B protein, human; D053583:Interleukin-1beta; D064446:Orexin Receptors; D011137:Polystyrenes; D011956:Receptors, Cell Surface; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II
keywords: Cytokine; Immunomodulation; Macrophage subsets; Polystyrene nanoparticles; Surface functionalization