title: A compact and integrated immunoassay with on-chip dispensing and magnetic particle handling.
abstract: We present a compact diagnostic platform for a rapid and sensitive detection of plasma biomarkers. The platform consists of a disposable microfluidic polymer chip, a processing device including a lens-free and cost efficient sensor system and a setup for dispersion of magnetic particles. The biomarkers of interest are quantified by magnetic bead based immunoassays with chemiluminescent readout technology. With a novel system for dispersion and manipulation of the magnetic particles in combination with chemiluminescence detection, the sensitivity of the immunoassay is improved and enables a rapid assay in a microfluidic format. In the disposable chip, extra chambers for storage and dispensing of biomarker specific reagents are integrated, which reduce the need of external dosing devices and thereby the cost of the platform is decreased. Plasma biomarkers for monitoring of sepsis could be quantified at 10 pg/mL concentrations within a total time of 30 min by the present system. This contribution is a fundamental step towards the development of an automatic and compact Point-of-Care testing device for monitoring of patients at the intensive care unit.
authors: Molecular Diagnostics, Health and Environment Department, Austrian Institute of Technology, Muthgasse 11/2, Vienna, Austria. helene.zirath.fl@ait.ac.at.|Ludwig Boltzmann Institute for Experimental and Clinical Traumatology at AUVA Research Center, Vienna, Austria. helene.zirath.fl@ait.ac.at.
authors: Helene
authors: H
authors: Zirath
authors: Molecular Diagnostics, Health and Environment Department, Austrian Institute of Technology, Muthgasse 11/2, Vienna, Austria.
authors: Johannes R
authors: JR
authors: Peham
authors: Biegler GmbH, Allhangstrasse 18a, 3001, Mauerbach, Austria.
authors: Guntram
authors: G
authors: Schnetz
authors: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology at AUVA Research Center, Vienna, Austria.
authors: Albert
authors: A
authors: Coll
authors: Inst. for Microsensors, -Actuators, & -Syst. (IMSAS/MCB), University of Bremen, Bremen, Germany.
authors: Lukas
authors: L
authors: Brandhoff
authors: Surgical Research Laboratories and Core Facility Flow Cytometry, Medical University of Vienna, Vienna, Austria.
authors: Andreas
authors: A
authors: Spittler
authors: Inst. for Microsensors, -Actuators, & -Syst. (IMSAS/MCB), University of Bremen, Bremen, Germany.
authors: Michael J
authors: MJ
authors: Vellekoop
authors: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology at AUVA Research Center, Vienna, Austria.
authors: Heinz
authors: H
authors: Redl
mesh_terms: D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D016209:Interleukin-8 / Q000097:blood*; D056656:Lab-On-A-Chip Devices; D060526:Magnetic Fields; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
keywords: Disposable polymer chip; Immunoassay; Inflammatory markers; Lab-on-a-chip; Magnetic particle handling; Microfluidics; On-chip dispensing; Point-of-Care