title: Caveolin-1 is upregulated in hepatic stellate cells but not sinusoidal endothelial cells after liver injury.
abstract: Sinusoidal endothelial cells (SEC) and hepatic stellate cells (HSC) are closely associated specialized vascular cells residing in the hepatic sinusoid. These cells have been shown to play important roles in many different pathophysiologic processes, in particular in liver fibrosis/cirrhosis and portal hypertension. Caveolin-1 functions as a scaffolding protein, and has a variety of functions including in many disease states, such as liver cirrhosis. Although previous studies have shown that in the injured rat liver, caveolin-1 is upregulated, the precise cells in which remains unclear. Therefore, the purpose of this study was to clarify the cell type (or types) in which caveolin-1 is expressed in normal and injured rat liver. We have utilized both detailed immunohistochemical labeling with cell specific markers as well as cell isolation techniques (isolating sinusoidal endothelial cells, HSCs, and hepatocytes) in normal and injured (bile duct ligation) rat liver. We show here that in the normal liver caveolin-1 is expressed predominantly in HSCs and SECs but after liver injury there is upregulation of caveolin-1 in HSCs, but not in SECs. These data have functional implications for the cells in which caveolin-1 is regulated.
authors: Medical University of South Carolina, Department of Medicine, Charleston, SC 29425, United States.
authors: Shweta
authors: S
authors: Singh
authors: Medical University of South Carolina, Department of Medicine, Charleston, SC 29425, United States.
authors: Songling
authors: S
authors: Liu
authors: Medical University of South Carolina, Department of Medicine, Charleston, SC 29425, United States. Electronic address: rockey@musc.edu.
authors: Don C
authors: DC
authors: Rockey
mesh_terms: D000818:Animals; D002196:Capillaries / Q000378:metabolism / Q000473:pathology; D051242:Caveolin 1 / Q000096:biosynthesis* / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D055166:Hepatic Stellate Cells / Q000378:metabolism* / Q000473:pathology; D022781:Hepatocytes / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008099:Liver / Q000098:blood supply / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats
chemical_list: D051242:Caveolin 1; D009569:Nitric Oxide
keywords: Immunoblotting; Immunohistochemistry; Nitric oxide; Signaling; eNOS