title: Human Decidua-Derived Mesenchymal Cells Are a Promising Source for the Generation and Cell Banking of Human Induced Pluripotent Stem Cells.
abstract: Placental tissue is a biomaterial with remarkable potential for use in regenerative medicine. It has a three-layer structure derived from the fetus (amnion and chorion) and the mother (decidua), and it contains huge numbers of cells. Moreover, placental tissue can be collected without any physical danger to the donor and can be matched with a variety of HLA types. The decidua-derived mesenchymal cells (DMCs) are highly proliferative fibroblast-like cells that express a similar pattern of CD antigens as bone marrow-derived mesenchymal cells (BM-MSCs). Here we demonstrated that induced pluripotent stem (iPS) cells could be efficiently generated from DMCs by retroviral transfer of reprogramming factor genes. DMC-hiPS cells showed equivalent characteristics to human embryonic stem cells (hESCs) in colony morphology, global gene expression profile (including human pluripotent stem cell markers), DNA methylation status of the OCT3/4 and NANOG promoters, and ability to differentiate into components of the three germ layers in vitro and in vivo. The RNA expression of XIST and the methylation status of its promoter region suggested that DMC-iPSCs, when maintained undifferentiated and pluripotent, had three distinct states: (1) complete X-chromosome reactivation, (2) one inactive X-chromosome, or (3) an epigenetic aberration. Because DMCs are derived from the maternal portion of the placenta, they can be collected with the full consent of the adult donor and have considerable ethical advantages for cell banking and the subsequent generation of human iPS cells for regenerative applications.
authors: Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization , Chuo-ku, Osaka , Japan.
authors: Tomoko
authors: T
authors: Shofuda
authors: † Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization , Osaka , Japan.
authors: Daisuke
authors: D
authors: Kanematsu
authors: † Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization , Osaka , Japan.
authors: Hayato
authors: H
authors: Fukusumi
authors: Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization , Chuo-ku, Osaka , Japan.
authors: Atsuyo
authors: A
authors: Yamamoto
authors: ‡ Department of Physiology, Keio University School of Medicine , Shinjuku-ku, Tokyo , Japan.
authors: Yohei
authors: Y
authors: Bamba
authors: § Department of Plastic Surgery, Osaka National Hospital, National Hospital Organization , Osaka , Japan.
authors: Sumiko
authors: S
authors: Yoshitatsu
authors: ¶ Biomedical Research Department, Central Institute for Experimental Animals , Kawasaki-ku, Kawasaki , Japan.
authors: Hiroshi
authors: H
authors: Suemizu
authors: ¶Biomedical Research Department, Central Institute for Experimental Animals, Kawasaki-ku, Kawasaki, Japan; #Department of Pathology and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
authors: Masato
authors: M
authors: Nakamura
authors: * Division of Chemotherapy, Faculty of Pharmacy, Keio University , Minato-ku, Tokyo , Japan.
authors: Yoshikazu
authors: Y
authors: Sugimoto
authors: †† Laboratory of Stem Cell Cultures, Laboratory of Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation , Ibaraki, Osaka , Japan.
authors: Miho Kusuda
authors: MK
authors: Furue
authors: ‡‡ JCRB Cell Bank, Laboratory of Cell Cultures, Research on Disease Bioresources, National Institute of Biomedical Innovation , Osaka , Japan.
authors: Arihiro
authors: A
authors: Kohara
authors: ‡ Department of Physiology, Keio University School of Medicine , Shinjuku-ku, Tokyo , Japan.
authors: Wado
authors: W
authors: Akamatsu
authors: ‡Department of Physiology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan; §§Kanrinmaru-Project, School of Medicine, Keio University, Tokyo, Japan.
authors: Yohei
authors: Y
authors: Okada
authors: ‡ Department of Physiology, Keio University School of Medicine , Shinjuku-ku, Tokyo , Japan.
authors: Hideyuki
authors: H
authors: Okano
authors: ¶¶Division of Molecular Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan; ##Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan; **Department of Pediatric Neurosurgery, Takatsuki General Hospital, Takatsuki, Osaka, Japan.
authors: Mami
authors: M
authors: Yamasaki
authors: †Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan; ##Department of Neurosurgery, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
authors: Yonehiro
authors: Y
authors: Kanemura
keywords: Decidua; Induced pluripotent stem cells (iPSCs); Mesenchymal cells; X-chromosome inactivation