title: Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum higginsianum.
abstract: After Colletotrichum storage methods in Chapter 23 , we describe here experimental methods for the inoculation of Colletotrichum higginsianum (C.h.) on Arabidopsis leaves. We put a particular focus on the methods for lesion measurements after the drop-inoculation of the leaves and on C.h. biomass measurements in the leaves by RT-qPCR analysis. As an option, we also briefly describe methods for counting C.h. entry ratio.
authors: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama-cho 8916-5, Ikoma, Nara, 630-0192, Japan.
authors: Kei
authors: K
authors: Hiruma
authors: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama-cho 8916-5, Ikoma, Nara, 630-0192, Japan. saijo@bs.naist.jp.|JST, Precursory Research for Embryonic Science and Technology, 45-3, Yonban-cho 5-3, Chiyoda-ku, Tokyo, 102-8666, Japan. saijo@bs.naist.jp.
authors: Yusuke
authors: Y
authors: Saijo
mesh_terms: D017360:Arabidopsis / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D020231:Colletotrichum / Q000472:pathogenicity*; D018515:Plant Leaves / Q000382:microbiology*
chemical_list: D029681:Arabidopsis Proteins
keywords: Arabidopsis; Biomass measurements; Colletotrichum higginsianum; Lesion measurements; RT-qPCR