title: Effects of saline-alkaline stress on benzo[a]pyrene biotransformation and ligninolytic enzyme expression by Bjerkandera adusta SM46.
abstract: Benzo[a]pyrene (BaP) accumulates in marine organisms and contaminated coastal areas. The biotreatment of waste water using saline-alkaline-tolerant white rot fungi (WRF) represents a promising method for removing BaP under saline-alkaline conditions based on WRF's ability to produce ligninolytic enzymes. In a pre-screening for degradation of polycyclic aromatic hydrocarbons of 82 fungal strains using Remazol brilliant blue R, Bjerkandera adusta SM46 exhibited the highest tolerance to saline-alkaline stress. Moreover, a B. adusta culture grown in BaP-containing liquid medium exhibited resistance to salinities up to 20 g l(-1). These conditions did not inhibit fungal growth or the expression of manganese peroxidase (MnP) or lignin peroxidase (LiP). The degradation rate also became higher as salinity increased to 20 g l(-1). Fungal growth and enzyme expression were inhibited at a salinity of 35 g l(-1). These inhibitory effects directly decreased the degradation rate (>24%). The presence of MnSO4 as an inducer improved the degradation rate and enzyme expression. MnP and LiP activity also increased by seven- and fivefold, respectively. SM46 degraded BaP (38-89% over 30 days) in an acidic environment (pH 4.5) and under saline-alkaline stress conditions (pH 8.2). Investigating the metabolites produced revealed BaP-1,6-dione as the main product, indicating the important role of ligninolytic enzymes in initializing BaP cleavage. The other metabolites detected, naphthalene acetic acid, hydroxybenzoic acid, benzoic acid, and catechol, may have been ring fission products. The wide range of activities observed suggests that B. adusta SM46 is a potential agent for biodegrading BaP under saline conditions.
authors: The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi Matsuyama, Ehime, 790-8566, Japan. adea002@lipi.go.id.|Research Centre for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor, Km. 46, Cibinong, Bogor, 16911, Indonesia. adea002@lipi.go.id.
authors: Ade
authors: A
authors: Andriani
authors: Department of Applied Bioscience, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan. tatibana@agr.ehime-u.ac.jp.
authors: Sanro
authors: S
authors: Tachibana
authors: Department of Applied Bioscience, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.
authors: Kazutaka
authors: K
authors: Itoh
mesh_terms: D000468:Alkalies / Q000633:toxicity*; D001564:Benzo(a)pyrene / Q000378:metabolism*; D001711:Biotransformation; D055453:Coriolaceae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D012492:Salts / Q000633:toxicity*; D013312:Stress, Physiological
chemical_list: D000468:Alkalies; D012492:Salts; D001564:Benzo(a)pyrene; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase
keywords: Benzo[a]pyrene; Biotransformation; Bjerkandera adusta; Ligninolytic enzymes; Saline waste water