title: Identification of Novel Pathways in Plant Lectin-Induced Cancer Cell Apoptosis.
abstract: Plant lectins have been investigated to elucidate their complicated mechanisms due to their remarkable anticancer activities. Although plant lectins seems promising as a potential anticancer agent for further preclinical and clinical uses, further research is still urgently needed and should include more focus on molecular mechanisms. Herein, a Naïve Bayesian model was developed to predict the protein-protein interaction (PPI), and thus construct the global human PPI network. Moreover, multiple sources of biological data, such as smallest shared biological process (SSBP), domain-domain interaction (DDI), gene co-expression profiles and cross-species interolog mapping were integrated to build the core apoptotic PPI network. In addition, we further modified it into a plant lectin-induced apoptotic cell death context. Then, we identified 22 apoptotic hub proteins in mesothelioma cells according to their different microarray expressions. Subsequently, we used combinational methods to predict microRNAs (miRNAs) which could negatively regulate the abovementioned hub proteins. Together, we demonstrated the ability of our Naïve Bayesian model-based network for identifying novel plant lectin-treated cancer cell apoptotic pathways. These findings may provide new clues concerning plant lectins as potential apoptotic inducers for cancer drug discovery.
authors: School of Basic Medical & Nursing School, Chengdu University, Chengdu 610106, China. drshiz1002@hotmail.com.
authors: Zheng
authors: Z
authors: Shi
authors: School of Life Sciences and Key Laboratory of Bio-resources, Ministry of Education, Sichuan University, Chengdu 610064, China. sunrong198982@163.com.
authors: Rong
authors: R
authors: Sun
authors: School of Basic Medical & Nursing School, Chengdu University, Chengdu 610106, China. yutian@edu.cn.
authors: Tian
authors: T
authors: Yu
authors: School of Basic Medical & Nursing School, Chengdu University, Chengdu 610106, China. liurongscu@126.com.
authors: Rong
authors: R
authors: Liu
authors: School of Basic Medical & Nursing School, Chengdu University, Chengdu 610106, China. chenglijia007@hotmail.com.
authors: Li-Jia
authors: LJ
authors: Cheng
authors: School of Life Sciences and Key Laboratory of Bio-resources, Ministry of Education, Sichuan University, Chengdu 610064, China. baojinku@scu.edu.cn.
authors: Jin-Ku
authors: JK
authors: Bao
authors: School of Basic Medical & Nursing School, Chengdu University, Chengdu 610106, China. zouliang@cdu.edu.cn.
authors: Liang
authors: L
authors: Zou
authors: School of Acupuncture and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. tangyong@cdutcm.edu.cn.
authors: Yong
authors: Y
authors: Tang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D019295:Computational Biology; D030562:Databases, Protein; D055808:Drug Discovery; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D008954:Models, Biological; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism*; D037121:Plant Lectins / Q000494:pharmacology*; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D035683:MicroRNAs; D037121:Plant Lectins; D012333:RNA, Messenger
keywords: Naïve Bayesian model; apoptosis; cancer; novel pathways; plant lectin