title: Long-noncoding RNAs in basal cell carcinoma.
abstract: Long noncoding RNAs (lncRNAs) are fundamental regulators of pre- and post-transcriptional gene regulation. Over 35,000 different lncRNAs have been described with some of them being involved in cancer formation. The present study was initiated to describe differentially expressed lncRNAs in basal cell carcinoma (BCC). Patients with BCC (n = 6) were included in this study. Punch biopsies were harvested from the tumor center and nonlesional epidermal skin (NLES, control, n = 6). Microarray-based lncRNA and mRNA expression profiles were identified through screening for 30,586 lncRNAs and 26,109 protein-coding transcripts (mRNAs). The microarray data were validated by RT-PCR in a second set of BCC versus control samples. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of mRNAs were performed to assess biologically relevant pathways. A total of 1851 lncRNAs were identified as being significantly up-regulated, whereas 2165 lncRNAs were identified as being significantly down-regulated compared to nonlesional skin (p < 0.05). Oncogenic and/or epidermis-specific lncRNAs, such as CASC15 or ANRIL, were among the differentially expressed sequences. GO analysis showed that the highest enriched GO targeted by up-regulated transcripts was "extracellular matrix." KEGG pathway analysis showed the highest enrichment scores in "Focal adhesion." BCC showed a significantly altered lncRNA and mRNA expression profile. Dysregulation of previously described lncRNAs may play a role in the molecular pathogenesis of BCC and should be subject of further analysis.
authors: Dermatologic Surgery Unit, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, 44791, Bochum, Germany. michael.sand@ruhr-uni-bochum.de.|Department of Plastic Surgery, St. Josef Hospital, Catholic Clinics of the Ruhr Peninsula, 45257, Essen, Germany. michael.sand@ruhr-uni-bochum.de.
authors: Michael
authors: M
authors: Sand
authors: Dermatologic Surgery Unit, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, 44791, Bochum, Germany.
authors: Falk G
authors: FG
authors: Bechara
authors: University of Michigan Kellogg Eye Center, Ann Arbor, MI, 48105, USA.
authors: Daniel
authors: D
authors: Sand
authors: Dermatologic Surgery Unit, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, 44791, Bochum, Germany.
authors: Thilo
authors: T
authors: Gambichler
authors: Department of Internal Medicine, Knappschaftskrankenhaus University of Bochum, Zentrum für Klinische Forschung, Labor für Molekulare Gastroenterologische Onkologie, 44780, Bochum, Germany.
authors: Stephan A
authors: SA
authors: Hahn
authors: Department of Plastic Surgery, St. Josef Hospital, Catholic Clinics of the Ruhr Peninsula, 45257, Essen, Germany.
authors: Michael
authors: M
authors: Bromba
authors: Dermatologic Surgery Unit, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, 44791, Bochum, Germany.
authors: Eggert
authors: E
authors: Stockfleth
authors: Dermatologic Surgery Unit, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, 44791, Bochum, Germany.
authors: Schapoor
authors: S
authors: Hessam
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002280:Basal Cell Carcinoma / Q000737:chemistry / Q000235:genetics*; D004817:Epidermis / Q000737:chemistry; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D006801:Humans; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000737:chemistry / Q000235:genetics*; D046888:Tissue Array Analysis
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D012334:RNA, Neoplasm
keywords: Basal cell carcinoma; Epithelial skin cancer; Long noncoding RNAs; Noncoding RNAs; Nonmelanoma skin cancer