title: MOLECULAR CHARACTERISATION AND ANTIMICROBIAL RESISTANCE PATTERNS OF ESCHERICHIA COLI ISOLATES FROM GOATS SLAUGHTERED IN PARTS OF KENYA.
abstract: OBJECTIVE
  To determine the antibiotic resistance patterns of pathogenic Escherichia coli on goat meat carcass at Huruma and Kiserian abattoirs in Kenya.
  
  
  METHODS
  Laboratory based study.
  
  
  METHODS
  Huruma and Kiserian abattoirs in Kenya,
  
  
  METHODS
  400 slaughtered goats inspected by veterinary health officers and approved for human consumption.
  
  
  METHODS
  A Total of 400 slaughtered goats which were inspected by veterinary health officers and approved for human consumption were sampled from Huruma and Kiserian abattoir. Goat carcass swabs were collected by passing each swab tissue on four parts of the carcass mainly neck, right and left forelimbs, right and left hind limbs, and brisket.
  
  
  RESULTS
  A total of 54 E. coli isolates were isolated and confirmed to be pathogenic. The percentage of isolates resistant to various microbial agents was recorded as follows: ampicillin (26 %), amoxycillin-clavulanic acid (17%), tetracycline (15%), chroramphenicol (4%), and ceftrixone (2% each). All Escherichia coli isolates were susceptible to gentamicin sulphamethaxazole-trimethomprin, kanamycin, cetriazididine (CAZ, 30pg), ciproxacin, nalidixic acid and chloramphenicol. Isolates were resistant to one or more of the antibiotics tested. Among the drugs tested, resistance was more frequently observed against ampicillin, amoxycillin-clavulanic acid, tetracycline, ceftrixone and chroramphenicol antibiotics. Among the isolates 26(48%) were positive for the stx1 gene, 19(35%) had the eae gene, 10(19%) possessed est gene,while 8(15%) harboured elt gene. Overall five isolates (10%) possessed aspu gene and two (4%) had aggR gene. No isolate possessed ipah gene.
  
  
  CONCLUSIONS
  This study demonstrated that there is a significant level of antimicrobial resistance in pathogenic E. coli isolated from goat meat from Huruma and Kiserian abattoir. This indicates that goat meat from abattoirs could pose a risk of transmission of pathogenic antibiotic resistant strains to human. Poor hygienic standards and indiscriminate use of antimicrobials are the two main reasons for the presence of resistant pathogens in goat carcasses.
  
  
  CONCLUSIONS
  Implemention of appropriate hygiene measures to control contamination of meat with pathogenic E. coli.
authors: Institute of Tropical Medicine and Infectious Diseases, Jomo Kenyatta University of Agriculture and Technology, PO Box 62000-00200, Nairobi, Kenya.
authors: S
authors: S
authors: Njoroge
authors: A W T
authors: AW
authors: Muigai
authors: P N
authors: PN
authors: Njiruh
authors: S
authors: S
authors: Kariuki
mesh_terms: D000003:Abattoirs / Q000592:standards; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000517:prevention & control; D006041:Goats; D064088:Hazard Analysis and Critical Control Points / Q000379:methods; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D008460:Meat / Q000382:microbiology / Q000592:standards; D008826:Microbial Sensitivity Tests; D020380:Needs Assessment
chemical_list: D000890:Anti-Infective Agents