title: A tumor-suppressive microRNA, miR-504, inhibits cell proliferation and promotes apoptosis by targeting FOXP1 in human glioma.
abstract: MicroRNAs (miRNAs) have been proposed as useful prognostic cancer biomarkers and as potential molecular targets for treating various cancers. Previous findings have indicated that miR-504 is dysregulated and involved in tumorigenesis of several types of cancer. However, the biological role of miR-504 in glioma remains unclear. In this study, we showed that miR-504 expression was markedly decreased in both glioma tissues and cell lines and that miR-504 downregulation significantly correlated with aggressive clinicopathological features and poor prognosis for glioma patients. In addition, miR-504 overexpression inhibited cell proliferation, induced cell cycle arrest, and promoted apoptosis in glioma cell lines. Furthermore, we identified forkhead box protein P1 (FOXP1) as a direct target of miR-504 using microarray analysis and a luciferase assay. Moreover, we demonstrated that miR-504 regulated glioma tumorigenesis by downregulating FOXP1 expression. Our results suggest that miR-504 might function as an important suppressor of glioma tumorigenesis and could serve as a promising candidate for therapeutic applications in glioma treatment.
authors: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Run
authors: R
authors: Cui
authors: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Yanlei
authors: Y
authors: Guan
authors: Department of Medical Photonics Research Center, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, 431-3192, Japan.
authors: Chuanqi
authors: C
authors: Sun
authors: Department of Geriatric Medicine, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Ling
authors: L
authors: Chen
authors: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Yijun
authors: Y
authors: Bao
authors: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Guangyu
authors: G
authors: Li
authors: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Bo
authors: B
authors: Qiu
authors: Department of Neurosurgery, Department of Geriatric Medicine, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Xin
authors: X
authors: Meng
authors: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.
authors: Chao
authors: C
authors: Pang
authors: Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China. Electronic address: wangyunjie_024@sina.com.
authors: Yunjie
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D057809:HEK293 Cells; D064593:Heterografts; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C486626:FOXP1 protein, human; D051858:Forkhead Transcription Factors; C550379:MIRN504 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins
keywords: Downregulation; Forkhead box protein P1; Glioma; miR-504; microRNA