title: Use of De Novo Transcriptome Libraries to Characterize a Novel Oleaginous Marine Chlorella Species during the Accumulation of Triacylglycerols.
abstract: Marine chlorophytes of the genus Chlorella are unicellular algae capable of accumulating a high proportion of cellular lipids that can be used for biodiesel production. In this study, we examined the broad physiological capabilities of a subtropical strain (C596) of Chlorella sp. "SAG-211-18" including its heterotrophic growth and tolerance to low salt. We found that the alga replicates more slowly at diluted salt concentrations and can grow on a wide range of carbon substrates in the dark. We then sequenced the RNA of Chlorella strain C596 to elucidate key metabolic genes and investigate the transcriptomic response of the organism when transitioning from a nutrient-replete to a nutrient-deficient condition when neutral lipids accumulate. Specific transcripts encoding for enzymes involved in both starch and lipid biosynthesis, among others, were up-regulated as the cultures transitioned into a lipid-accumulating state whereas photosynthesis-related genes were down-regulated. Transcripts encoding for two of the up-regulated enzymes-a galactoglycerolipid lipase and a diacylglyceride acyltransferase-were also monitored by reverse transcription quantitative polymerase chain reaction assays. The results of these assays confirmed the transcriptome-sequencing data. The present transcriptomic study will assist in the greater understanding, more effective application, and efficient design of Chlorella-based biofuel production systems.
authors: Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY, United States of America.
authors: Cresten B
authors: CB
authors: Mansfeldt
authors: Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY, United States of America.
authors: Lubna V
authors: LV
authors: Richter
authors: Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY, United States of America.
authors: Beth A
authors: BA
authors: Ahner
authors: Romberg Tiburon Center for Environmental Studies, San Francisco State University, Tiburon, CA, United States of America.
authors: William P
authors: WP
authors: Cochlan
authors: Department of Civil and Environmental Engineering, Cornell University, Ithaca, NY, United States of America.
authors: Ruth E
authors: RE
authors: Richardson
mesh_terms: D053898:Biosynthetic Pathways; D002708:Chlorella / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D005786:Gene Expression Regulation; D050356:Lipid Metabolism; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D059467:Transcriptome; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D012337:RNA, Ribosomal, 18S; D014280:Triglycerides