title: In Situ Potentiometry and Ellipsometry: A Promising Tool to Study Biofouling of Potentiometric Sensors.
abstract: In situ potentiometry and null ellipsometry was combined and used as a tool to follow the kinetics of biofouling of ion-selective electrodes (ISEs). The study was performed using custom-made solid-contact K(+)-ISEs consisting of a gold surface with immobilized 6-(ferrocenyl)hexanethiol as ion-to-electron transducer that was coated with a potassium-selective plasticized polymer membrane. The electrode potential and the ellipsometric signal (corresponding to the amount of adsorbed protein) were recorded simultaneously during adsorption of bovine serum albumin (BSA) at the surface of the K(+)-ISEs. This in situ method may become useful in developing sensors with minimized biofouling.
authors: Johan Gadolin Process Chemistry Centre, c/o Laboratory of Analytical Chemistry, Åbo Akademi University , FIN-20500 Åbo-Turku, Finland.|Department of Biomedical Sciences, Faculty of Health and Society, Malmö University , SE-205 06 Malmö, Sweden.|Biofilms-Research Center for Biointerfaces, Malmö University , SE-205 06 Malmö, Sweden.
authors: Grzegorz
authors: G
authors: Lisak
authors: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University , SE-205 06 Malmö, Sweden.|Biofilms-Research Center for Biointerfaces, Malmö University , SE-205 06 Malmö, Sweden.
authors: Thomas
authors: T
authors: Arnebrant
authors: Johan Gadolin Process Chemistry Centre, c/o Laboratory of Analytical Chemistry, Åbo Akademi University , FIN-20500 Åbo-Turku, Finland.|Faculty of Materials Science and Ceramics, AGH-University of Science and Technology , 30-059 Krakow, Poland.
authors: Andrzej
authors: A
authors: Lewenstam
authors: Johan Gadolin Process Chemistry Centre, c/o Laboratory of Analytical Chemistry, Åbo Akademi University , FIN-20500 Åbo-Turku, Finland.
authors: Johan
authors: J
authors: Bobacka
authors: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University , SE-205 06 Malmö, Sweden.|Biofilms-Research Center for Biointerfaces, Malmö University , SE-205 06 Malmö, Sweden.
authors: Tautgirdas
authors: T
authors: Ruzgas
mesh_terms: D057219:Biofouling; D011199:Potentiometry / Q000295:instrumentation*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D012710:Serum Albumin, Bovine