title: Isolation Efficiency of Mouse Pancreatic Stem Cells Is Age Dependent.
abstract: Mouse pancreatic stem cells have been isolated from mouse pancreata. This study evaluated the efficacy of isolating mouse pancreatic stem cells using mice of different ages. The pancreata of newborn mice, 8-week-old mice, and 24-week-old mice were harvested and digested by using collagenase. The "duct-like" cells in the digested pancreatic tissue were then inoculated into 96-well plates, cloned by limiting dilution, and cultured in DMEM with 20% FBS. Pancreatic stem cells were isolated from the pancreata of all newborn mice, while cells could only be isolated from 10% of the pancreata of 8-week-old mice and could not be isolated from the pancreata of any 24-week-old mice. These data suggest that young mice may have some pancreatic stem cells and that older mice may only have a few pancreatic stem cells. These data also indicate that it is extremely difficult to isolate pancreatic stem cells from older mice, suggesting that future research focus its efforts on finding methods of isolating pancreatic stem cells from adult mice.
authors: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Takashi
authors: T
authors: Kuise
authors: † Department of Surgery, Clinical Research Center, Chiba-East Hospital, National Hospital Organization , Chiba , Japan.
authors: Hirofumi
authors: H
authors: Noguchi
authors: ‡ Department of Pediatric Dentistry, Niigata University Graduate School of Medical and Dental Sciences , Niigata , Japan.
authors: Issei
authors: I
authors: Saitoh
authors: § Department of Primary Care and Medical Education, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Hitomi Usui
authors: HU
authors: Kataoka
authors: ¶ Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Masami
authors: M
authors: Watanabe
authors: # Department of Socio-environmental Design, Hiroshima International University , Hiroshima , Japan.
authors: Yasufumi
authors: Y
authors: Noguchi
authors: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Toshiyoshi
authors: T
authors: Fujiwara
keywords: Age dependent; Diabetes; ES medium; Feeder cells; Mouse pancreatic stem cells; Pancreatic islet transplantation