title: RNA interference of cytochrome P450 CYP6F subfamily genes affects susceptibility to different insecticides in Locusta migratoria.
abstract: BACKGROUND
  Many insect cytochrome P450s (CYPs) play critical roles in detoxification of insecticides. The CYP6 family is unique to the class Insecta, and its biochemical function has essentially been associated with the metabolism of xenobiotics. In this study, we sequenced and characterised the full-length cDNAs of five CYP genes from Locusta migratoria, a highly destructive agricultural pest worldwide.
  
  
  RESULTS
  The five genes were predominantly expressed in brain, guts, fat bodies or Malpighian tubules. CYP6FE1, CYP6FF1 and CYP6FG1 were expressed at higher levels in fourth-instar nymphs than in other developmental stages. CYPFD2 is specifically expressed in adults, whereas CYP6FD1, CYP6FD2 and CYP6FE1 showed significantly lower expression in eggs than in other developmental stages. Deltamethrin suppressed CYP6FD1 expression in third-instar nymphs and upregulated the expression level of CYP6FD2, CYP6FF1 and CYP6FG1 at the dose of LD10. Efficient RNA interference-mediated gene silencing was established for four of the five CYP genes. Silencing of CYP6FF1 increased the nymphal mortality from 23 to 50% in response to deltamethrin. Silencing of CYP6FD2 and CYP6FE1 increased the nymphal mortality from 32 to 72 and 66%, respectively, to carbaryl.
  
  
  CONCLUSIONS
  Three of the four CYP6F subfamily genes in L. migratoria were associated with the detoxification of deltamethrin or carbaryl. The role of CYPs in insecticide detoxification appears to be both gene and insecticide specific. © 2016 Society of Chemical Industry.
authors: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.|College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors: Yanqiong
authors: Y
authors: Guo
authors: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.
authors: Haihua
authors: H
authors: Wu
authors: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.
authors: Xueyao
authors: X
authors: Zhang
authors: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.
authors: Enbo
authors: E
authors: Ma
authors: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China.
authors: Yaping
authors: Y
authors: Guo
authors: Department of Entomology, Kansas State University, Manhattan, KS, USA. kzhu@ksu.edu.
authors: Kun Yan
authors: KY
authors: Zhu
authors: Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China. zjz@sxu.edu.cn.
authors: Jianzhen
authors: J
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000072472:Cytochrome P450 Family 6 / Q000235:genetics* / Q000378:metabolism; D008658:Inactivation, Metabolic; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D048188:Locusta migratoria / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009758:Nymph / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009928:Organ Specificity; D010802:Phylogeny; D034622:RNA Interference; D016415:Sequence Alignment
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D000072472:Cytochrome P450 Family 6
keywords: Locusta migratoria; RNA interference; cytochrome P450; insecticide detoxification