title: A complex water network contributes to high-affinity binding in an antibody-antigen interface.
abstract: This data article presents an analysis of structural water molecules in the high affinity interaction between a potent tumor growth inhibiting antibody (fragment), J22.9-xi, and the tumor marker antigen CD269 (B cell maturation antigen, BCMA). The 1.89 Å X-ray crystal structure shows exquisite details of the binding interface between the two molecules, which comprises relatively few, mostly hydrophobic, direct contacts but many indirect interactions over solvent waters. These are partly or wholly buried in, and therefore part of, the interface. A partial description of the structure is included in an article on the tumor inhibiting effects of the antibody: "Potent anti-tumor response by targeting B cell maturation antigen (BCMA) in a mouse model of multiple myeloma", Mol. Oncol. 9 (7) (2015) pp. 1348-58.
authors: Max Delbrueck Centrum for Molecular Medicine, Berlin, Germany.
authors: S F
authors: SF
authors: Marino
authors: Max Delbrueck Centrum for Molecular Medicine, Berlin, Germany.
authors: D
authors: D
authors: Olal
authors: Max Delbrueck Centrum for Molecular Medicine, Berlin, Germany.
authors: O
authors: O
authors: Daumke
keywords: BCMA; Binding interface; Crystal structure; Fab fragment; High affinity; Water molecules