title: A Glance at Recombination Hotspots in the Domestic Cat.
abstract: Recombination has essential roles in increasing genetic variability within a population and in ensuring successful meiotic events. The objective of this study is to (i) infer the population-scaled recombination rate (ρ), and (ii) identify and characterize regions of increased recombination rate for the domestic cat, Felis silvestris catus. SNPs (n = 701) were genotyped in twenty-two East Asian feral cats (random bred). The SNPs covered ten different chromosomal regions (A1, A2, B3, C2, D1, D2, D4, E2, F2, X) with an average region size of 850 Kb and an average SNP density of 70 SNPs/region. The Bayesian method in the program inferRho was used to infer regional population recombination rates and hotspots localities. The regions exhibited variable population recombination rates and four decisive recombination hotspots were identified on cat chromosome A2, D1, and E2 regions. As a description of the identified hotspots, no correlation was detected between the GC content and the locality of recombination spots, and the hotspots enclosed L2 LINE elements and MIR and tRNA-Lys SINE elements.
authors: College of Science, Department of Biological Sciences, Kuwait University, Safat, 13060, Kuwait.
authors: Hasan
authors: H
authors: Alhaddad
authors: Department of Bioinformatics and Genetics, Swedish Museum of Natural History, Box 50007, SE-104 05, Stockholm, Sweden.
authors: Chi
authors: C
authors: Zhang
authors: Department of Evolution and Ecology, University of California Davis, Davis, CA, 95616, United States of America.
authors: Bruce
authors: B
authors: Rannala
authors: Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO, 65211, United States of America.
authors: Leslie A
authors: LA
authors: Lyons
mesh_terms: D000818:Animals; D001482:Base Composition; D001499:Bayes Theorem; D002415:Cats / Q000235:genetics*; D002681:China; D005260:Female; D005828:Genetics, Population; D020084:Long Interspersed Nucleotide Elements; D008297:Male; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D011995:Recombination, Genetic; D020086:Short Interspersed Nucleotide Elements