title: Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development.
abstract: Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells of small and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults.
authors: Centre of Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, La Plata, Argentina.
authors: V A
authors: VA
authors: Ferretti
authors: Centre of Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, La Plata, Argentina.
authors: A
authors: A
authors: Segal-Eiras
authors: Laboratory of Histology and Embryology, Faculty of Veterinary Sciences, National University of La Plata, Argentina.
authors: C G
authors: CG
authors: Barbeito
authors: Centre of Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, La Plata, Argentina. Electronic address: crocevir@hotmail.com.
authors: M V
authors: MV
authors: Croce
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development; D041981:Gastrointestinal Tract / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D055271:Mucin 5AC / Q000235:genetics* / Q000378:metabolism; D055262:Mucin-2 / Q000235:genetics* / Q000378:metabolism; D051381:Rats / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012137:Respiratory System / Q000254:growth & development / Q000378:metabolism*
chemical_list: D055271:Mucin 5AC; D055262:Mucin-2
keywords: Development; Embryology; Muc2; Muc5ac; Rat