title: Evaluation of genotoxic variations in plant model systems in a case of metal stressors.
abstract: This study was designed to assess the effects of long term, high metal exposition (cadmium, lead, copper, nickel and zinc) on DNA damage in four plant model systems [Taraxacum officinale (Asteraceae), Matricaria recutita L. (Asteraceae), Robinia pseudoacacia L. (Fabaceae), and Urtica dioica (Urticaceae)]. DNA stability was investigated by a Random Amplified Polymorphic DNA (RAPD) technique. Agarose-gel electrophoresis revealed total of 37 bands with different molecular weights ranging from 1250 to 5000 bp. It generated distinctive polymorphism value of 72.97% (27 bands) total in four plant species investigated. The dendrogram constructed using NTSYSpc programme showed that there is grouping in separate clusters of the same plant model collected from two different areas (metal-exposed and control samples). The study concluded that the long term metal-exposing periods had genotoxic stress on macromolecules of plant model systems investigated and biomarkers used should be augmented for reliable estimates of genotoxicity after exposure of plants to metal stressors.
authors: a Faculty of Medical Sciences, Goce Delčev University , Štip , Republic of Macedonia.
authors: Darinka Gjorgieva
authors: DG
authors: Ackova
authors: b Faculty of Pharmacy, Ss. Cyril and Methodius University , Skopje , Republic of Macedonia.
authors: Tatjana
authors: T
authors: Kadifkova-Panovska
authors: c Research Center for Environment and Materials, Macedonian Academy of Sciences and Arts , Skopje, Republic of Macedonia, Skopje , Macedonia.
authors: Katerina Bačeva
authors: KB
authors: Andonovska
authors: d Institute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University , Skopje , Republic of Macedonia.
authors: Trajče
authors: T
authors: Stafilov
mesh_terms: D002104:Cadmium / Q000032:analysis; D003300:Copper / Q000032:analysis; D004249:DNA Damage / Q000187:drug effects*; D004785:Environmental Pollutants / Q000032:analysis*; D007854:Lead / Q000032:analysis; D031168:Matricaria / Q000737:chemistry; D019216:Metals, Heavy / Q000032:analysis*; D009153:Mutagens / Q000032:analysis*; D009532:Nickel / Q000032:analysis; D010944:Plants / Q000737:chemistry* / Q000235:genetics*; D018843:Republic of North Macedonia; D031301:Robinia / Q000737:chemistry; D031205:Taraxacum / Q000737:chemistry; D026982:Urtica dioica / Q000737:chemistry; D015032:Zinc / Q000032:analysis
chemical_list: D004785:Environmental Pollutants; D019216:Metals, Heavy; D009153:Mutagens; D002104:Cadmium; D007854:Lead; D003300:Copper; D009532:Nickel; D015032:Zinc
keywords: RAPD; genotoxic effects; metals; plants