title: FISH and AgNor mapping of the 45S and 5S rRNA genes in wild and cultivated species of Capsicum (Solananceae).
abstract: Chromosome number and position of rDNA were studied in 12 wild and cultivated species of the genus Capsicum with chromosome numbers x = 12 and x = 13 (22 samples). For the first time in these species, the 5S and 45S rRNA loci were localized and physically mapped using two-color fluorescence in situ hybridization and AgNOR banding. We focused on the comparison of the results obtained with both methods with the aim of accurately revealing the real functional rRNA genes. The analyzes were based on a previous work that reported that the 18S-5.8S-25S loci mostly coincide with GC-rich heterochromatic regions and likely have given rise to satellite DNAs, which are not active genes. These data show the variability of rDNA within karyotypes of the genus Capsicum, providing anchor points for (comparative) genetic maps. In addition, the obtained information might be useful for studies on evolution of repetitive DNA.
authors: a Instituto Multidisciplinario de Biología Vegetal (IMBIV), CONICET and Universidad Nacional de Córdoba, CC 495, CP 5000, Córdoba, Argentina.|b Facultad de Ciencias Exactas, Físicas y Naturales (FCEFyN), Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, CP 5000, Córdoba, Argentina.
authors: Marisel A
authors: MA
authors: Scaldaferro
authors: c Instituto de Biologia, Universidade Estadual de Campinas-UNICAMP, Brasil.
authors: M Victoria Romero
authors: MV
authors: da Cruz
authors: d Molecular Genetics and Cell Biology, The University of Chicago, 929 East 57th Street GCIS Room W519P, Chicago, USA.
authors: Nicolás M
authors: NM
authors: Cecchini
authors: a Instituto Multidisciplinario de Biología Vegetal (IMBIV), CONICET and Universidad Nacional de Córdoba, CC 495, CP 5000, Córdoba, Argentina.
authors: Eduardo A
authors: EA
authors: Moscone
mesh_terms: D002212:Capsicum / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D017343:Genes, Plant; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D009697:Nucleolus Organizer Region / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
chemical_list: D018749:RNA, Plant; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; C088179:RNA, ribosomal, 45S
keywords: 45S rRNA genes; 5S rRNA genes; AgNOR banding; Capsicum chromosomes; cartographie physique; coloration AgNOR; fluorescence in situ hybridization; gènes de l’ARNr 45S; gènes de l’ARNr 5S; hybridation in situ en fluorescence; physical gene mapping