title: Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model.
abstract: Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS.
authors: AntiCancer, Inc., San Diego, CA, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, United States of America.|Department of Gastroenterological Surgery, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Shuya
authors: S
authors: Yano
authors: AntiCancer, Inc., San Diego, CA, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, United States of America.|Department of Gastroenterological Surgery, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Kiyoto
authors: K
authors: Takehara
authors: AntiCancer, Inc., San Diego, CA, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, United States of America.
authors: Shinji
authors: S
authors: Miwa
authors: Department of Gastroenterological Surgery, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Hiroyuki
authors: H
authors: Kishimoto
authors: AntiCancer, Inc., San Diego, CA, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, United States of America.
authors: Yukihiko
authors: Y
authors: Hiroshima
authors: AntiCancer, Inc., San Diego, CA, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, United States of America.
authors: Takashi
authors: T
authors: Murakami
authors: Oncolys BioPharm Inc., Tokyo, Japan.
authors: Yasuo
authors: Y
authors: Urata
authors: Department of Gastroenterological Surgery, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Shunsuke
authors: S
authors: Kagawa
authors: Department of Surgery, University of California San Diego, San Diego, CA, United States of America.
authors: Michael
authors: M
authors: Bouvet
authors: Department of Gastroenterological Surgery, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Toshiyoshi
authors: T
authors: Fujiwara
authors: AntiCancer, Inc., San Diego, CA, United States of America.|Department of Surgery, University of California San Diego, San Diego, CA, United States of America.
authors: Robert M
authors: RM
authors: Hoffman
mesh_terms: D000256:Adenoviridae; D000818:Animals; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000556:secondary / Q000601:surgery; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D019098:Telomerase
chemical_list: D049452:Green Fluorescent Proteins; D019098:Telomerase