title: Altered Loyalties of Neuronal Markers in Cultured Slices of Resected Human Brain Tissue.
abstract: Organotypic cultures from normal neocortical tissue obtained at epilepsy surgery show a severe injury response. This response involves both neuronal degeneration and the proliferation of reactive cells. A salient feature of the reactive cells is the co-expression of microglial and astrocytic markers. Surprisingly, the reactive cells also began to express neuronal markers Tubulin βIII and MAP2 adding to the confusion about their origin. Concomitant with their appearance in reactive cells MAP2 and Tubulin βIII expression disappeared from neurons. While NeuN expression decreased significantly, it did not entirely disappear from many neurons. Moreover, it was not observed in reactive cells, showing that NeuN is a reliable marker of neurons.
authors: Department of Neuropsychiatric Disorders, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), Amsterdam, The Netherlands.
authors: Ronald W H
authors: RW
authors: Verwer
authors: Department of Neuropsychiatric Disorders, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), Amsterdam, The Netherlands.
authors: Arja A
authors: AA
authors: Sluiter
authors: Department of Neuropsychiatric Disorders, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), Amsterdam, The Netherlands.
authors: Rawien A
authors: RA
authors: Balesar
authors: Department of Neurosurgery, VU University Medical Center, Amsterdam, The Netherlands.
authors: Johannes C
authors: JC
authors: Baayen
authors: Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors: Dave
authors: D
authors: Speijer
authors: Department of Neurosurgery, VU University Medical Center, Amsterdam, The Netherlands.
authors: Sander
authors: S
authors: Idema
authors: Department of Neuropsychiatric Disorders, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), Amsterdam, The Netherlands.
authors: Dick F
authors: DF
authors: Swaab
mesh_terms: D034961:Antigens, Nuclear / Q000032:analysis / Q000096:biosynthesis*; D015415:Biomarkers / Q000032:analysis*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism*; D009924:Organ Culture Techniques; D013702:Temporal Lobe / Q000378:metabolism*
chemical_list: D034961:Antigens, Nuclear; D015415:Biomarkers; D009419:Nerve Tissue Proteins; C506808:neuronal nuclear antigen NeuN, human
keywords: brain injury; epilepsy; human neocortex; neuronal degeneration; neuronal markers; reactive glial cells; tissue culture