title: Fusion of platelet-derived growth factor receptor β to CEV14 gene in chronic myelomonocytic leukemia: A case report and review of the literature.
abstract: Myeloid tumor possessing platelet-derived growth factor receptor β (PDGFRβ) gene rearrangement is a rare hematological malignancy, which presents with typical characteristics of myeloid proliferation disorders and eosinophilia. In the present study, an elderly chronic myelomonocytic leukemia patient was diagnosed with chromosome rearrangement. Fluorescencein situhybridization (FISH) was conducted with a PDGFRβ isolate probe, and gene translocation between PDGFRβ on chromosome 5 and genes on the chromosomes of group D (13-15) was detected. Karyotype analysis revealed a chromosome 5 break, and PDGFRβ-thyroid hormone receptor interactor 11 (CEV14) gene fusion was confirmed via reverse transcription-polymerase chain reaction (RT-PCR), which additionally revealed the chromosome rearrangement t(5;14)(q33;q32). Due to the correlation between PDGFRβ-CEV14 expression and effectiveness of treatment with tyrosine kinase inhibitors, this fusion gene is considered to be an oncogene. In the present study, an elderly patient was diagnosed with a myeloid tumor associated with the fusion gene PDGFRβ-CEV14, using the methods of FISH and RT-PCR. These methods were confirmed to be of significant value in improving diagnosis, guiding treatment and increasing the cure rate of patients, due to their ability to detect multiple rearrangement genes associated with PDGFRβ in myelodysplastic and myeloproliferative neoplasms.
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Sheng-Lan
authors: SL
authors: Gong
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Meng-Qiao
authors: MQ
authors: Guo
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Gu-Sheng
authors: GS
authors: Tang
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Chun-Ling
authors: CL
authors: Zhang
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Hui-Ying
authors: HY
authors: Qiu
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Xiao-Xia
authors: XX
authors: Hu
authors: Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors: Jian-Min
authors: JM
authors: Yang
keywords: chromosome rearrangement; chronic myelomonocytic leukemia; gene fusion; platelet-derived growth factor receptor β