title: Identification of human exercise-induced physiological function changes based on bioinformatics analysis.
abstract: BACKGROUND
  This study aimed to explore the physiological functions that were significantly affected by the exercise training.
  
  
  METHODS
  Microarray dataset of GSE53598 was downloaded and preprocessed to identify the differentially expressed genes (DEGs) between pre- and post-exercise muscle biopsies. Then hierarchical clustering, protein-protein interaction (PPI) network construction and functional enrichment analysis were conducted. Finally, the significant physiological functions were identified using logistic regression algorithm.
  
  
  RESULTS
  Total 420 up- and 420 down-regulated DEGs were identified. DEGs including E2F transcription factor 6, hypocretin (Orexin) neuropeptide precursor, caveolin 1, platelet-derived growth factor beta polypeptide, and transforming growth factor, beta 1 had higher degree in the PPI network. Functions related to blood vessel development, regulation of cell migration, negative regulation of gene expression, and negative regulation of metabolic process changed significantly after exercise.
  
  
  CONCLUSIONS
  Functions related to blood vessel development, regulation of cell migration, negative regulation of gene expression and negative regulation of metabolic process may be closely associated with exercising.
authors: Rehabilitation Medicine Department, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Liquan
authors: L
authors: Yin
authors: Rehabilitation Medicine Department, China-Japan Union Hospital of Jilin University, Changchun, China - caifeifeiei@hotmail.com.
authors: Fei
authors: F
authors: Cai
authors: Emergency Department, China-Japan Union Hospital of Jilin University, Changchun, China.
authors: Dong
authors: D
authors: Wang
authors: Digestive Department, Jilin Province People's Hospital, Changchun, China.
authors: Jiao
authors: J
authors: Ge
mesh_terms: D019295:Computational Biology / Q000379:methods*; D015536:Down-Regulation; D015444:Exercise / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D018482:Muscle, Skeletal / Q000502:physiology*