title: Spirolactone and spirothiolactone rhodamine-pyrene probes for detection of Hg²⁺ with different sensing properties and its application in living cells.
abstract: Two new rhodamine B-based fluorescent probes PyRbS and PyRbO containing pyrene moiety were designed and synthesized. Both of the probes showed colorimetric and fluorometric sensing abilities for Hg(2+) with high selectivity over other metal ions. The binding analysis using Job's plot suggested 1:1 stoichiometry for the complexes formed for Hg(2+). Compared with PyRbO, the PyRbS showed higher selectivity and sensitivity due to the thiophilic property of Hg(2+) ion. The PyRbS exhibited the linear fluorescence quenching to Hg(2+) in the range of 0.3 to 4.8 μM (λ(ex)=365 nm) and 0.3 to 5.4 μM (λ(ex)=515 nm), and the detection limit was 0.72 μM. Moreover, ratiometric changes of PyRbS with Hg(2+) in absorption spectrum were observed, which could not be obtained in the combination of PyRbO with Hg(2+). In addition, the methyl thiazolyl tetrazolium (MTT) assay demonstrated that RbPyS had low cytotoxicity and was successfully used to monitor intracellular Hg(2+) levels in living cells.
authors: College of Science, Nanjing Tech University, Nanjing 211816, China.
authors: Qing-Qing
authors: QQ
authors: Rui
authors: College of Science, Nanjing Tech University, Nanjing 211816, China.
authors: Yi
authors: Y
authors: Zhou
authors: College of Science, Nanjing Tech University, Nanjing 211816, China.
authors: Yuan
authors: Y
authors: Fang
authors: College of Science, Nanjing Tech University, Nanjing 211816, China; State Key Laboratory of Coordination Chemistry, Nanjing University, Jiangsu 210093, China. Electronic address: yaocheng@njtech.edu.cn.
authors: Cheng
authors: C
authors: Yao
mesh_terms: D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D061986:MCF-7 Cells; D008628:Mercury / Q000032:analysis*; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods; D011721:Pyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013148:Spironolactone / Q000138:chemical synthesis / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011721:Pyrenes; D012235:Rhodamines; D013438:Sulfhydryl Compounds; D013148:Spironolactone; C030984:pyrene; D008628:Mercury; C029773:rhodamine B
keywords: Fluorescent probe; Hg(2+) ion; Live-cell imaging; Rhodamine B