title: Integrative microRNA and gene profiling data analysis reveals novel biomarkers and mechanisms for lung cancer.
abstract: BACKGROUND
  Studies on the accuracy of microRNAs (miRNAs) in diagnosing non-small cell lung cancer (NSCLC) have still controversial. Therefore, we conduct to systematically identify miRNAs related to NSCLC, and their target genes expression changes using microarray data sets.
  
  
  METHODS
  We screened out five miRNAs and six genes microarray data sets that contained miRNAs and genes expression in NSCLC from Gene Expression Omnibus.
  
  
  RESULTS
  Our analysis results indicated that fourteen miRNAs were significantly dysregulated in NSCLC. Five of them were up-regulated (miR-9, miR-708, miR-296-3p, miR-892b, miR-140-5P) while nine were down-regulated (miR-584, miR-218, miR-30b, miR-522, miR486-5P, miR-34c-3p, miR-34b, miR-516b, miR-592). The integrating diagnosis sensitivity (SE) and specificity (SP) were 82.6% and 89.9%, respectively. We also found that 4 target genes (p < 0.05, fold change > 2.0) were significant correlation with the 14 discovered miRNAs, and the classifiers we built from one training set predicted the validation set with higher accuracy (SE = 0.987, SP = 0.824).
  
  
  CONCLUSIONS
  Our results demonstrate that integrating miRNAs and target genes are valuable for identifying promising biomarkers, and provided a new insight on underlying mechanism of NSCLC. Further, our well-designed validation studies surely warrant the investigation of the role of target genes related to these 14 miRNAs in the prediction and development of NSCLC.
authors: Department of Anesthesiology, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan, China.|Department of Internal Medicine and Biochemistry, Rush University Medical Center, Chicago, IL, USA.
authors: Ling
authors: L
authors: Hu
authors: Department of Internal Medicine and Biochemistry, Rush University Medical Center, Chicago, IL, USA.
authors: Junmei
authors: J
authors: Ai
authors: Department of Gastroenterology, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan, China.
authors: Hui
authors: H
authors: Long
authors: Department of Orthopedics, Pu Ai Hospital, Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors: Weijun
authors: W
authors: Liu
authors: Department of Biological Science and Technology, Wuhan Bioengineering Institute, Wuhan, China.
authors: Xiaomei
authors: X
authors: Wang
authors: Department of Orthopedic, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan, China.
authors: Yi
authors: Y
authors: Zuo
authors: Department of Internal Medicine and Biochemistry, Rush University Medical Center, Chicago, IL, USA.
authors: Yan
authors: Y
authors: Li
authors: Medical College, Wuhan University of Science and Technology, Wuhan, China.
authors: Qingming
authors: Q
authors: Wu
authors: Medical College, Wuhan University of Science and Technology, Wuhan, China.|Department of Internal Medicine and Biochemistry, Rush University Medical Center, Chicago, IL, USA.
authors: Youping
authors: Y
authors: Deng
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger
keywords: biomarker; lung cancer; meta-analysis; microRNAs; target gene