title: Induction and differentiation of adipose-derived stem cells from human buccal fat pads into salivary gland cells.
abstract: Atrophy or hypofunction of the salivary gland because of aging or disease leads to hyposalivation that affects patient quality of life by causing dry mouth, deterioration of mastication/deglutition, and poor oral hygiene status. Current therapy for atrophy or hypofunction of the salivary gland in clinical practice focuses on symptom relief using drugs and artificial saliva; therefore, there is still a need to develop new therapies. To investigate potential novel therapeutic targets, we induced the differentiation of salivary gland cells by co-culturing human adipose-derived stem cells isolated from buccal fat pads (hBFP-ASCs) with human salivary-gland-derived fibroblasts (hSG-fibros). We examined their potential for transplantation and tissue neogenesis. Following the culture of hBFP-ASCs and hSG-fibros, differentiated cells were transplanted into the submandibular glands of SCID mice, and their degree of differentiation in tissues was determined. We also examined their potential for functional tissue reconstitution using a three-dimensional (3D) culture system. Co-cultured cells expressed salivary-glandrelated markers and generated new tissues following transplantation in vivo. Moreover, cell reconstituted glandular structures in the 3D culture system. In conclusion, coculture of hSG-fibros with hBFP-ASCs led to successful differentiation into salivary gland cells that could be transplanted to generate new tissues.
authors: Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan. miu-k@tky.ndu.ac.jp.|Department of NDU Life Sciences, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-0071, Japan. miu-k@tky.ndu.ac.jp.
authors: Miyuki
authors: M
authors: Kawakami
authors: Department of NDU Life Sciences, School of Life Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-0071, Japan.
authors: Hiroshi
authors: H
authors: Ishikawa
authors: Department of Oral and Maxillofacial Surgery, Niigata Hospital, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.|Division of Cell Regeneration and Transplantation, Advanced Research Center, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
authors: Akira
authors: A
authors: Tanaka
authors: Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Niigata, The Nippon Dental University, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.
authors: Izumi
authors: I
authors: Mataga
mesh_terms: D017667:Adipocytes; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001284:Atrophy; D002454:Cell Differentiation; D002478:Cells, Cultured; D002610:Cheek; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D016513:Mice, SCID; D012469:Salivary Glands / Q000166:cytology* / Q000473:pathology / Q000503:physiopathology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D014987:Xerostomia / Q000188:drug therapy / Q000209:etiology
keywords: ASCs; Buccal fat pads; Fibroblasts; Regenerative; Salivary glands