title: Modulation of Protein S-Nitrosylation by Isoprene Emission in Poplar.
abstract: Researchers have been examining the biological function(s) of isoprene in isoprene-emitting (IE) species for two decades. There is overwhelming evidence that leaf-internal isoprene increases the thermotolerance of plants and protects them against oxidative stress, thus mitigating a wide range of abiotic stresses. However, the mechanisms of abiotic stress mitigation by isoprene are still under debate. Here, we assessed the impact of isoprene on the emission of nitric oxide (NO) and the S-nitroso-proteome of IE and non-isoprene-emitting (NE) gray poplar (Populus × canescens) after acute ozone fumigation. The short-term oxidative stress induced a rapid and strong emission of NO in NE compared with IE genotypes. Whereas IE and NE plants exhibited under nonstressful conditions only slight differences in their S-nitrosylation pattern, the in vivo S-nitroso-proteome of the NE genotype was more susceptible to ozone-induced changes compared with the IE plants. The results suggest that the nitrosative pressure (NO burst) is higher in NE plants, underlining the proposed molecular dialogue between isoprene and the free radical NO Proteins belonging to the photosynthetic light and dark reactions, the tricarboxylic acid cycle, protein metabolism, and redox regulation exhibited increased S-nitrosylation in NE samples compared with IE plants upon oxidative stress. Because the posttranslational modification of proteins via S-nitrosylation often impacts enzymatic activities, our data suggest that isoprene indirectly regulates the production of reactive oxygen species (ROS) via the control of the S-nitrosylation level of ROS-metabolizing enzymes, thus modulating the extent and velocity at which the ROS and NO signaling molecules are generated within a plant cell.
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Elisa
authors: E
authors: Vanzo
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Juliane
authors: 0000-0002-3422-4083
authors: J
authors: Merl-Pham
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Violeta
authors: V
authors: Velikova
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Andrea
authors: A
authors: Ghirardo
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Christian
authors: C
authors: Lindermayr
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Stefanie M
authors: SM
authors: Hauck
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Jörg
authors: 0000-0001-8410-5624
authors: J
authors: Bernhardt
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Katharina
authors: K
authors: Riedel
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.).
authors: Jörg
authors: J
authors: Durner
authors: Helmholtz Zentrum München, Research Unit Environmental Simulation (E.V., V.V., A.G., J.-P.S.), Institute of Biochemical Plant Pathology (C.L., J.D.), and Research Unit Protein Science (J.M.-P., S.M.H.), D-85764 Neuherberg, Germany;Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria (V.V.); andInstitute for Microbiology, Ernst-Moritz-Arndt University, 17487 Greifswald, Germany (J.B., K.R.) jp-schnitzler@helmholtz-muenchen.de.
authors: Jörg-Peter
authors: 0000-0002-9825-867X
authors: JP
authors: Schnitzler
mesh_terms: D002070:Butadienes / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D005651:Fumigation; D005838:Genotype; D045782:Hemiterpenes / Q000378:metabolism*; D016018:Least-Squares Analysis; D009569:Nitric Oxide / Q000378:metabolism; D015538:Nitrosation; D010126:Ozone / Q000494:pharmacology; D010420:Pentanes / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D032107:Populus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D013312:Stress, Physiological / Q000187:drug effects; D013997:Time Factors
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; D010940:Plant Proteins; D020543:Proteome; C005059:isoprene; D009569:Nitric Oxide; D010126:Ozone; D002244:Carbon