title: Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria.
abstract: Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca(2+)-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.
authors: Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russia.
authors: Elena V
authors: EV
authors: Eremeeva
authors: Laboratory of Biochemistry, Wageningen University, The Netherlands.
authors: Willem J H
authors: WJ
authors: van Berkel
authors: Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russia.
authors: Eugene S
authors: ES
authors: Vysotski
mesh_terms: D000465:Algorithms; D000818:Animals; D001059:Apoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D044482:Benzeneacetamides / Q000378:metabolism; D060807:Bioluminescence Resonance Energy Transfer Techniques; D020013:Calcium Signaling; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D037402:Hydrozoa / Q000737:chemistry / Q000502:physiology*; D007093:Imidazoles / Q000378:metabolism; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D011719:Pyrazines / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D001059:Apoproteins; D044482:Benzeneacetamides; D007093:Imidazoles; D008164:Luminescent Proteins; D011719:Pyrazines; D011994:Recombinant Proteins; C542661:coelenteramide; C046165:phialidin; D049452:Green Fluorescent Proteins; C017144:coelenterazine
keywords: FRET; aequorin; bioluminescence; coelenterazine; obelin; protein-protein interaction