title: Synergistic infection of two viruses MCMV and SCMV increases the accumulations of both MCMV and MCMV-derived siRNAs in maize.
abstract: The co-infection of Maize chlorotic mottle virus (MCMV) and Sugarcane mosaic virus (SCMV) can cause maize lethal necrosis. However, the mechanism underlying the synergistic interaction between these two viruses remains elusive. In this study, we found that the co-infection of MCMV and SCMV increased the accumulation of MCMV. Moreover, the profiles of virus-derived siRNAs (vsiRNAs) from MCMV and SCMV in single- and co-infected maize plants were obtained by high-throughput sequencing. Our data showed that synergistic infection of MCMV and SCMV increased remarkably the accumulation of vsiRNAs from MCMV, which were mainly 22 and 21 nucleotides in length. The single-nucleotide resolution maps of vsiRNAs revealed that vsiRNAs were almost continuously but heterogeneously distributed throughout MCMV and SCMV genomic RNAs, respectively. Moreover, we predicted and annotated dozens of host transcript genes targeted by vsiRNAs. Our results also showed that maize DCLs and several AGOs RNAs were differentially accumulated in maize plants with different treatments (mock, single or double inoculations), which were associated with the accumulation of vsiRNAs. Our findings suggested possible roles of vsiRNAs in the synergistic interaction of MCMV and SCMV in maize plants.
authors: State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
authors: Zihao
authors: Z
authors: Xia
authors: State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
authors: Zhenxing
authors: Z
authors: Zhao
authors: State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
authors: Ling
authors: L
authors: Chen
authors: State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
authors: Mingjun
authors: M
authors: Li
authors: State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
authors: Tao
authors: T
authors: Zhou
authors: Beijing Entry-exit Inspection and Quarantine Bureau, Beijing 100026, China.
authors: Congliang
authors: C
authors: Deng
authors: Chinese Society of Inspection and Quarantine, Beijing 100026, China.
authors: Qi
authors: Q
authors: Zhou
authors: State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China.
authors: Zaifeng
authors: Z
authors: Fan
mesh_terms: D060565:Argonaute Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D060085:Coinfection; D018506:Gene Expression Regulation, Plant; D015967:Gene Expression Regulation, Viral; D063990:Gene Ontology; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D017864:Tombusvirus / Q000235:genetics* / Q000472:pathogenicity; D003313:Zea mays / Q000821:virology*
chemical_list: D060565:Argonaute Proteins; D018797:Cell Cycle Proteins; D010940:Plant Proteins; D034741:RNA, Small Interfering; D012367:RNA, Viral