title: The VviMYB80 Gene is Abnormally Expressed in Vitis vinifera L. cv. 'Zhong Shan Hong' and its Expression in Tobacco Driven by the 35S Promoter Causes Male Sterility.
abstract: Anther development is a very precise and complicated process. In Arabidopsis, the AtMYB80 transcription factor regulates genes involved in pollen development and controls the timing of tapetal programmed cell death (PCD). In this study, we isolated and characterized cDNA for VviMYB80 expressed in flower buds of male-sterile Vitis vinifera L. cv. 'Zhong Shan Hong', a late-maturing cultivar derived from self-progeny of cv. 'Wink'. VviMYB80 belongs to the MYB80 subfamily and clusters with AtMYB35/TDF1 in a distinct clade. We found that in flower buds, expression of the VviMYB80 gene in cv. 'Zhong Shan Hong' sharply increased at the tetrad stage, resulting in a higher and earlier transcript level than that found in cv. 'Wink'. Expression of the VviMYB80 gene, driven by the 35S promoter, caused pleiotropic effects on the stamens, including smaller and shriveled anthers, delayed dehiscence, fewer seeds, shorter anther filaments, distorted pollen shape and a lack of cytoplasm, with the tapetum exhibiting hypertrophy in transformed tobacco. These results suggest that VviMYB80 may play an important role in stamen development and that expression of VviMYB80 driven by the 35S promoter in tobacco induces male sterility.
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo, 113-8657 Japan.
authors: Huan
authors: H
authors: Zheng
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China.
authors: Xiaojuan
authors: X
authors: Yu
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China.
authors: Yue
authors: Y
authors: Yuan
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China.
authors: Yaguang
authors: Y
authors: Zhang
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China.
authors: Zhen
authors: Z
authors: Zhang
authors: Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing, 210095 PR China.
authors: Jiyu
authors: J
authors: Zhang
authors: Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, Tokyo, 113-8657 Japan.
authors: Meng
authors: M
authors: Zhang
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China.
authors: Chenfei
authors: C
authors: Ji
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China.
authors: Qian
authors: Q
authors: Liu
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095 PR China taojianmin@njau.edu.cn.
authors: Jianmin
authors: J
authors: Tao
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002473:Cell Wall / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D035264:Flowers / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018525:Germination; D010641:Phenotype; D010802:Phylogeny; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020005:Propanols / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D027843:Vitis / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D020005:Propanols; D014157:Transcription Factors; C439395:1-phenylpropanol
keywords: Electron microscopy; Histological observations; Male sterility; Phenylpropanoid pathway; Real-time qPCR; Vitis vinifera