title: Mechanism of diethylhexylphthalate (DEHP) induced testicular damage and of grape seed extract-induced protection in the rat.
abstract: The aim of this study was to determine the effect of diethylhexylphthalate (DEHP) on testicular mitochondrial viability and lipid peroxidation as a possible novel mechanism of PEHP testicular toxicity and whether grape seed extract (GSE) beneficially influences the mitochondrial function in testes of rats exposed to diethylhexylphthalate (DEHP). Sixty male albino rats were divided into three groups (n = 20): group I: was used as a control, group II: received diethylhexylphthalate (DEHP) (500 mg/kg/day orally) alone for 30 days, and group III: received the same DEHP dose in combination with GSE (proanthocyanidins) (100 mg/kg body weight). DEHP administration significantly decreases the testicular mitochondrial viability, mRNA expression of androgen receptors (AR), testosterone hormone concentration, increases mRNA expression of INOS and as compared to control group. It also decreases reduced glutathione (GSH) concentration, glutathione reductase (GR), super oxide dismutase (SOD), Catalase activities and increases lipid peroxidation (LPO) and DNA fragmentation%. In synchronization, a substantial decrease of testicular & epididymal weight and volume which accompanied by considerable alteration of semen character. Grape seed extract (GSE) alleviates the toxic effects of DEHP by increasing the mitochondrial viability, decreases the lipid peroxidation, and increases the testicular antioxidant activity. Our results were confirmed by histopathological and immunhistochemical studies.
authors: Department of Histology, Faculty of Medicine, Beni-Suef University, Egypt.
authors: Samraa H
authors: SH
authors: Abdel-Kawi
authors: Department of Biochemistry, Faculty of Veterinary Medicine, Beni-Suef University, Egypt. Electronic address: khalid_hashem@yahoo.com.
authors: Khalid S
authors: KS
authors: Hashem
authors: Theriogenology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
authors: Saber
authors: S
authors: Abd-Allah
mesh_terms: D000272:Adipates / Q000633:toxicity*; D000818:Animals; D005786:Gene Expression Regulation; D056604:Grape Seed Extract / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013733:Testicular Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control; D013737:Testis / Q000187:drug effects / Q000473:pathology
chemical_list: D000272:Adipates; D056604:Grape Seed Extract; D012333:RNA, Messenger; C013966:dioctyl adipate
keywords: Androgen receptors; Antioxidant activity.; DEHP; GSE; Mitochondrial viability; Testosterone hormone