title: Behavior of Human Articular Chondrocytes During In Vivo Culture in Closed, Permeable Chambers.
abstract: The exact contribution of transplanted chondrocytes for cartilage tissue repair prior expansion in monolayer culures remains undetermined. At our laboratory, we have created a new permeable chamber to study the chondrogenesis of dedifferentiated cells implanted ectopically in a closed and controlled environment. The behavior of chondrocytes has been studied in settings frequently used in clinical approaches during transplantation, namely injection of autologous chondrocyte cells in suspension (ACI), cells soaked in collagen membranes (MACI), and cells applied in a polymer gel (fibrin). As controls, we have tested the redifferentiation of chondrocytes in cell aggregates, and we have checked the proper functionality of chambers both in vitro and in vivo. After retrieval, firmed tissue-like shapes were recovered only from chambers containing cells seeded in membranes. Histomorphological, immunohistochemical, and ultrastructural analyses revealed synthesis of fibrous-like tissue, characterized by low-density collagen fibers, low collagen type II, abundant collagen type I, and low amounts of proteoglycans. Additionally, neither the collagen membranes nor the fibrin gel was reabsorbed by cells. In summary, our results show that the newly developed permeable chambers function correctly, allowing proper cell feeding and preventing cell leakage or host cell invasion. Additionally, our results suggest that, under these circumstances, chondrocytes are not able to orchestrate formation of hyaline cartilage and have little capacity to degrade artificial membranes or carrier gels such as fibrin. These are interesting observations that should be considered for understanding what role the transplanted chondrocytes play during restoration of articular cartilage after implantation.
authors: Orthopaedic Surgery Department, Institute of Clinical Medicine, University of Tromsø , Tromsø , Norway.
authors: Iñigo
authors: I
authors: Martinez-Zubiaurre
authors: † Scaffdex Oy , Tampere , Finland.
authors: Tuija
authors: T
authors: Annala
authors: Orthopaedic Surgery Department, Institute of Clinical Medicine, University of Tromsø, Tromsø, Norway; ‡Orthopaedic Surgery Department, University Hospital of North Norway, Tromsø, Norway.
authors: Martin
authors: M
authors: Polacek
keywords: Cartilage; Chondrogenesis; Closed permeable chambers; Collagen membranes