title: Bioanalytical challenges and improved detection of circulating levels of IL-13.
abstract: BACKGROUND
  IL-13 is a key mediator of type 2 inflammation-driven diseases. Circulating IL-13 levels are very low and challenging to detect reliably. We assessed the ability of immunoassays on the Erenna(®) and IMPACT platforms to measure serum IL-13 in asthma, idiopathic pulmonary fibrosis (IPF) and atopic dermatitis (AD) patients and in healthy controls (HC).
  
  
  RESULTS
  The Erenna IL-13 assay exhibited significant specificity issues and had limited ability to detect IL-13 in serum samples. The IMPACT IL-13 assay had excellent specificity and detected IL-13 in 100% of serum samples tested from asthma, IPF and AD patients and HC. Serum IL-13 levels were significantly elevated in asthma, IPF and AD patients, relative to HC.
  
  
  CONCLUSIONS
  The IMPACT IL-13 assay had fg/ml sensitivity and excellent specificity, enabling reliable detection of circulating levels of IL-13.
authors: ITGR/OMNI Biomarker Development, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors: Fang
authors: F
authors: Cai
authors: Roche Professional Diagnostics, Nonnenwald 2, 82377 Penzberg, Germany.
authors: Hans
authors: H
authors: Hornauer
authors: BioAnalytical Sciences, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors: Kun
authors: K
authors: Peng
authors: ITGR/OMNI Biomarker Development, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors: Cheryl A
authors: CA
authors: Schofield
authors: ITGR/OMNI Biomarker Development, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors: Heleen
authors: H
authors: Scheerens
authors: ITGR/OMNI Biomarker Development, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
authors: Alyssa M
authors: AM
authors: Morimoto
mesh_terms: D001249:Asthma / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000097:blood*; D006801:Humans; D018793:Interleukin-13 / Q000097:blood*; D057230:Limit of Detection; D011658:Pulmonary Fibrosis / Q000097:blood; D013997:Time Factors
chemical_list: D018793:Interleukin-13
keywords: AD; IPF; asthma; sensitivity; serum IL-13; specificity; type 2 inflammation