title: Checking for reversibility of aggregation of UV-irradiated glycogen phosphorylase b under crowding conditions.
abstract: It is believed that the initial stages of protein aggregation are reversible and can be reversed by simple dilution, whereas prolonged exposure to factors responsible for denaturing proteins (for example, to elevated temperatures) results in the formation of irreversible aggregates. A new approach has been developed to discriminate the stage of the formation of reversible aggregates. Aggregation of UV-irradiated glycogen phosphorylase b (UV-Phb) was studied at 10, 25 and 37 °C in the presence of crowders (polyethylene glycol and Ficoll-70) using dynamic light scattering and analytical ultracentrifugation (pH 6.8; 0.1M NaCl). The dilution of the protein solution in the course of aggregation at 10 °C results in the breakdown of protein aggregates suggesting that the aggregation process is reversible. When aggregation of UV-Phb is studied at 37 °C, reversibility is lacking. Chemical chaperones (arginine, proline) induce the breakdown of protein aggregates of UV-Phb formed at 10 °C. In the experiments carried out at 37 °C in the presence of crowder the addition of arginine results in disintegration of protein aggregates only at early stages of the aggregation process. It is assumed that general pathway of protein aggregation includes the formation of reversible, completely dissociable, partly dissociable and irreversible aggregates.
authors: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2, Leninsky Ave., Moscow 119071, Russia. Electronic address: eronina@inbi.ras.ru.
authors: Tatiana B
authors: TB
authors: Eronina
authors: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2, Leninsky Ave., Moscow 119071, Russia.
authors: Valeriya V
authors: VV
authors: Mikhaylova
authors: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2, Leninsky Ave., Moscow 119071, Russia.
authors: Natalia A
authors: NA
authors: Chebotareva
authors: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2, Leninsky Ave., Moscow 119071, Russia.
authors: Valentina F
authors: VF
authors: Makeeva
authors: Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2, Leninsky Ave., Moscow 119071, Russia. Electronic address: kurganov@inbi.ras.ru.
authors: Boris I
authors: BI
authors: Kurganov
mesh_terms: D000818:Animals; D005362:Ficoll / Q000494:pharmacology; D024982:Glycogen Phosphorylase, Muscle Form / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000494:pharmacology; D066329:Protein Aggregates / Q000187:drug effects* / Q000528:radiation effects*; D011817:Rabbits; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D066329:Protein Aggregates; D005362:Ficoll; D011092:Polyethylene Glycols; D024982:Glycogen Phosphorylase, Muscle Form
keywords: Arginine; Crowding; Glycogen phosphorylase b; Protein aggregation; Reversibility