title: Aeromonas lusitana sp. nov., Isolated from Untreated Water and Vegetables.
abstract: During previous studies to evaluate the phylogenetic diversity of Aeromonas from untreated waters and vegetables intended for human consumption, a group of isolates formed a unique gyrB phylogenetic cluster, separated from those of all other species described so far. A subsequent extensive phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene sequencing, multi-locus phylogenetic analysis of the concatenated sequence of seven housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX, and atpD; 4705 bp), and ERIC-PCR, were performed in an attempt to ascertain the taxonomy position of these isolates. This polyphasic approach confirmed that they belonged to a novel species of the genus Aeromonas, for which the name Aeromonas lusitana sp. nov. is proposed, with strain A.11/6(T) (=DSMZ 24095(T), =CECT 7828(T)) as the type strain.
authors: Area de Microbiología, EPSO, Universidad Miguel Hernández, 03312, Orihuela, Alicante, Spain. ammurcia@umh.es.|Genetic PCR Solutions TM, CEEI, Elche, Alicante, Spain. ammurcia@umh.es.
authors: Antonio
authors: A
authors: Martínez-Murcia
authors: Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
authors: Roxana
authors: R
authors: Beaz-Hidalgo
authors: Genetic PCR Solutions TM, CEEI, Elche, Alicante, Spain.
authors: Aaron
authors: A
authors: Navarro
authors: Department of Biology & CESAM, University of Aveiro, 3810-193, Aveiro, Portugal.
authors: M João
authors: MJ
authors: Carvalho
authors: School of Pathology and Laboratory Medicine, The University of Western Australia Crawley, Crawley, WA, Australia.
authors: Max
authors: M
authors: Aravena-Román
authors: Department of Biology & CESAM, University of Aveiro, 3810-193, Aveiro, Portugal.
authors: Antonio
authors: A
authors: Correia
authors: Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
authors: M José
authors: MJ
authors: Figueras
authors: Department of Veterinary Sciences, CECAV-University of Trás-os-Montes e Alto Douro, Vila Real, Portugal.
authors: M José
authors: MJ
authors: Saavedra
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005506:Food Contamination / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D006801:Humans; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014675:Vegetables / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S