title: A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system.
abstract: The enteric nervous system, the largest division of the peripheral nervous system, is derived from vagal neural crest cells that invade and populate the entire length of the gut to form diverse neuronal subtypes. Here, we identify a novel population of neurons within the enteric nervous system of zebrafish larvae that express the transgenic marker ptf1a:GFP within the midgut. Genetic lineage analysis reveals that enteric ptf1a:GFP(+) cells are derived from the neural crest and that most ptf1a:GFP(+) neurons express the neurotransmitter 5HT, demonstrating that they are serotonergic. This transgenic line, Tg(ptf1a:GFP), provides a novel neuronal marker for a subpopulation of neurons within the enteric nervous system, and highlights the possibility that Ptf1a may act as an important transcription factor for enteric neuron development.
authors: California Institute of Technology, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California.
authors: Rosa A
authors: RA
authors: Uribe
authors: California Institute of Technology, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California.
authors: Tiffany
authors: T
authors: Gu
authors: California Institute of Technology, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California.
authors: Marianne E
authors: ME
authors: Bronner
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D017615:Enteric Nervous System / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008822:Mice, Transgenic; D009432:Neural Crest / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000254:growth & development*
chemical_list: D011994:Recombinant Proteins; D014157:Transcription Factors; C062602:transcription factor PTF1; D049452:Green Fluorescent Proteins; D012701:Serotonin
keywords: enteric nervous system; ptf1a; serotonergic neuron; zebrafish