title: Nanoscale Metal-Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells.
abstract: We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal-organic framework (NMOF), R-UiO, as an intracellular oxygen (O2) sensor. R-UiO contains a Pt(II)-porphyrin ligand as an O2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O2-insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O2 partial pressure. In vitro experiments confirmed the applicability of R-UiO as an intracellular O2 biosensor. This work is the first report of a NMOF-based intracellular oxygen sensor and should inspire the design of ratiometric NMOF sensors for other important analytes in biological systems.
authors: Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.
authors: Ruoyu
authors: R
authors: Xu
authors: Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.|Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology , Shanghai 200237, China.
authors: Youfu
authors: Y
authors: Wang
authors: Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.
authors: Xiaopin
authors: X
authors: Duan
authors: Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.
authors: Kuangda
authors: K
authors: Lu
authors: Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.
authors: Daniel
authors: D
authors: Micheroni
authors: Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology , Shanghai 200237, China.
authors: Aiguo
authors: A
authors: Hu
authors: Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.
authors: Wenbin
authors: W
authors: Lin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D015374:Biosensing Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival; D008665:Metalloporphyrins / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D010100:Oxygen / Q000032:analysis*
chemical_list: D001672:Biocompatible Materials; D008665:Metalloporphyrins; D010100:Oxygen