title: Gold nanoparticles and bioconjugation: a pathway for proteomic applications.
abstract: In the last few years gold nanoparticles (AuNPs) became extremely interesting materials due to their enhanced optical, chemical and electrical properties. With the intention of taking advantage of those properties, the use of AuNPs has spread into a wide variety of areas such as physics, chemistry, biology, industry and medicine. More interestingly, their ability to form robust conjugates with biomolecules has given proteomics a new tool to improve aspects where the current methods to study proteins and their interactions in living cells cannot achieve the success required. In this review we present some of the current methods for AuNPs synthesis, the tailoring of their surface with ligands to improve stability and strategies to conjugate with biomolecules. Lastly, we also discuss their application in proteomic methods and recent developments in clinical diagnosis.
authors: a CICECO-Aveiro Institute of Materials, Department of Chemistry , University of Aveiro , 3810-193 Aveiro , Portugal.
authors: Cláudia
authors: C
authors: Couto
authors: b Mass Spectrometry Center, Organic Chemistry, Natural and Agro-Food Products Research Unit (QOPNA), Department of Chemistry , University of Aveiro , 3810-193 Aveiro , Portugal.|c Department of Medical Sciences, iBiMED - Institute for Biomedicine , University of Aveiro , 3810-193 Aveiro , Portugal and.|d Department of Physiology and Cardiothoracic Surgery, Faculty of Medicine , University of Porto , Porto , Portugal.
authors: Rui
authors: R
authors: Vitorino
authors: a CICECO-Aveiro Institute of Materials, Department of Chemistry , University of Aveiro , 3810-193 Aveiro , Portugal.
authors: Ana L
authors: AL
authors: Daniel-da-Silva
mesh_terms: D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles; D040901:Proteomics
chemical_list: D006046:Gold
keywords: Biosensor; functionalization; gold nanoparticles; ligand; mass spectrometry; protein; protein enrichment; synthesis