title: [In vitro activity of ampicillin-ceftriaxone against Enterococcus faecalis isolates recovered from invasive infections].
abstract: In vitro activity of the combination of ampicillin- ceftriaxone against 30 Enterococcus faecalis isolates recovered from invasive infections in patients admitted to Hospital de Clínicas José de San Martin in the city of Buenos Aires was assessed. Ampicillin- ceftriaxone synergies were determined by microdilution in Müeller-Hinton (MH) broth with and without subinhibitory concentrations of ceftriaxone. Synergy was detected in 22/30 isolates. A decrease in both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was observed in 14/30 isolates, whereas in 6/30 isolates the decrease was observed in the MIC value and only in the MBC value in the 2 remaining isolates. The bactericidal activity of the combination showed to be higher at low concentrations of ampicillin (< 1 μg/ml). We detected in vitro synergy using the ampicillin-ceftriaxone combination and thus, its efficacy was confirmed in the treatment of severe infections by E. faecalis.
authors: Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, INFIBIOC, UBA, Buenos Aires, Argentina.
authors: Noelia
authors: N
authors: Burguer Moreira
authors: Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, INFIBIOC, UBA, Buenos Aires, Argentina.
authors: Marcela
authors: M
authors: Nastro
authors: Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, INFIBIOC, UBA, Buenos Aires, Argentina.
authors: Carlos
authors: C
authors: Vay
authors: Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, INFIBIOC, UBA, Buenos Aires, Argentina.
authors: Ángela
authors: Á
authors: Famiglietti
authors: Laboratorio de Bacteriología, Departamento de Bioquímica Clínica, Hospital de Clínicas José de San Martín, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, INFIBIOC, UBA, Buenos Aires, Argentina. Electronic address: carlos_hernanrodriguez@hotmail.com.
authors: Carlos Hernán
authors: CH
authors: Rodríguez
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D002443:Ceftriaxone / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D002443:Ceftriaxone; D000667:Ampicillin
keywords: Ampicilina-ceftriaxona; Ampicillin-ceftriaxone; Enterococcus faecalis; Sinergia; Synergy