title: Label-Free Quantitation for Clinical Proteomics.
abstract: Label-free quantification (LFQ) has emerged as a viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations, and mass spectrometric performance must be highly reproducible for this approach to be effective. The following protocols describe the key steps in the methods related to sample preparation and analysis for LC-MS/MS-based label-free quantitation using standard data-dependent acquisition.
authors: Centre for Biotechnology, University of Turku, Biocity, Tykistökatu 6, Turku, Finland. rmoulder@btk.fi.
authors: Robert
authors: R
authors: Moulder
authors: Mass Spectrometry Center, University of Maryland-Baltimore, 20 North Pine Street, Baltimore, MD, USA. ygoo@rx.umaryland.edu.
authors: Young Ah
authors: YA
authors: Goo
authors: Mass Spectrometry Center, University of Maryland-Baltimore, 20 North Pine Street, Baltimore, MD, USA. dgoodlett@rx.umaryland.edu.
authors: David R
authors: DR
authors: Goodlett
mesh_terms: D002853:Chromatography, Liquid; D006801:Humans; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
keywords: Area under the curve (AUC); Label-free quantification; Mass spectrometry; Proteomics