title: In vitro activity of 3β-O-tigloylmelianol from Guarea kunthiana A. Juss (Meliaceae) on oogenesis and ecdysis of the cattle tick Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae).
abstract: We evaluated the effects of 3β-O-tigloylmelianol from Guarea kunthiana A. Juss (Meliaceae) on oogenesis, as a larvicide and on ecdysis of the larvae and the nymphs of the cattle tick Rhipicephalus (Boophilus) microplus (Canestrini) (Acari: Ixodidae). On the oogenesis' test, 48 engorged females were divided into three groups, evaluated at 24, 48 and 72 h post-treatment. Half of the females were treated with 0.01% 3β-O-tigloylmelianol diluted in distilled water and 5% dimethyl sulfoxide (DMSO), while the other half (controls) were exposed to distilled water and 5% DMSO. After treatment, the ovaries were weighed in order to measure the gonadosomatic index (GSI) and were also subjected to standard histological technical tests. On the larvicide and ecdysis' tests, 3β-O-tigloylmelianol was tested at concentrations of 0.01, 0.005, 0.0025 and 0.00125%. Compared with the controls, there was a reduction of GSI of approximately 50% on the treated group, which started at 48 h post treatment. Overall, the protolimonoid 3β-O-tigloylmelianol has caused a significant reduction in the number of oocytes. It has also caused alteration of the cytoplasmic and germinal vesicle diameters. Morphological changes, such as vacuolization, chorion irregularity which has modified the oocytes' morphology as well as alterations on the yolk's granules were also observed. The compound was not larvicide, however, interfered in the ecdysis of the larvae and the nymphs. This study shows that the protolimonoid 3β-O-tigloylmelianol from G. kunthiana acts on oogenesis and ecdysis of R. (B.) microplus, but not as larvicide, indicating that it acts on the endocrine system of the tick.
authors: Laboratório de Parasitologia Animal, Universidade Estadual de Mato Grosso do Sul, Aquidauana, MS, Brazil. Electronic address: csmangarosa@gmail.com.
authors: Carolina da Silva
authors: Cda S
authors: Barbosa
authors: Departamento de Microbiologia, Imunologia, Parasitologia e Patologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors: Lígia Miranda Ferreira
authors: LM
authors: Borges
authors: Instituto Federal Goiano, Urutaí, GO, Brazil.
authors: Carla Cristina Braz
authors: CC
authors: Louly
authors: Laboratório de Comportamento Celular, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors: Thiago Lopes
authors: TL
authors: Rocha
authors: Laboratório de Comportamento Celular, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors: Simone Maria Teixeira
authors: SM
authors: de Sabóia-Morais
authors: Laboratório de Pesquisa de Produtos Naturais Bioativos, Instituto de Química, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors: Carlos Henrique
authors: CH
authors: Miguita
authors: Laboratório de Pesquisa de Produtos Naturais Bioativos, Instituto de Química, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors: Walmir Silva
authors: WS
authors: Garcez
authors: Laboratório de Pesquisa de Produtos Naturais Bioativos, Instituto de Química, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors: Fernanda Rodriguez
authors: FR
authors: Garcez
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D004305:Dose-Response Relationship, Drug; D005260:Female; D036701:Limonins / Q000494:pharmacology*; D029582:Meliaceae / Q000737:chemistry*; D018978:Molting / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure; D009866:Oogenesis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011817:Rabbits; D048494:Rhipicephalus / Q000187:drug effects* / Q000502:physiology; D013984:Tick Infestations / Q000469:parasitology / Q000662:veterinary
chemical_list: C000601870:3beta-O-tigloylmelianol; D036701:Limonins; D010936:Plant Extracts
keywords: Integrated pest management; Medicinal plants; Pest control; Phytotherapy; Protolimonoids