title: A potential anti-inflammation activity and depigmentation effect of Lespedeza bicolor extract and its fractions.
abstract: Postinflammatory hyperpigmentation (PIH) is an acquire hypermelanosis after cutaneous inflammation and injury. The aim of the present study was to investigate a natural ingredient with the anti-inflammatory and depigmentation activities into possible applications of postinflammatory hyperpigmentation. Methanol extracts of Lespedeza bicolor and its various fractions inhibited LPS-induced NO production in RAW 264.7 macrophages in a concentration-dependent manner. In particular, the ethyl acetate fraction was shown to be inhibition of NO production (89%) and down-regulation of iNOS mRNA without causing cytotoxicity. In addition, ethyl acetate fraction significantly attenuated LPS-induced NF-κB activation (P < 0.05), indicating the anti-inflammatory activity due to NF-κB inhibition. Moreover, extracts, mainly ethyl acetate fraction, exhibited not only DPPH free radical scavenging activity (IC50, 112.45 μg/mL) with 4 times lower activity than ascorbic acid, but also anti-tyrosinase activity (IC50, 1 μg/mL) with a similar activity to arbutin showing a competitive inhibitor. Furthermore, vitexin and haginins A, B and C were identified through LC-MS analysis as potential compounds responsible for these effects. These results suggest that L. bicolor extract have anti-inflammatory, antioxidant activities and tyrosinase inhibitory effect and it might be used in the management of postinflammatory pigmentation through inhibition of pathogenic process involved in hyperpigmentation.
authors: Laboratory of Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Seung Jin
authors: SJ
authors: Lee
authors: Laboratory of Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: M D Akil
authors: MD
authors: Hossaine
authors: Laboratory of Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors: Seung Chun
authors: SC
authors: Park
keywords: Anti-inflammation; Depigmentation; Lespedeza bicolor; NF-κB; Tyrosinase activity; iNOS