title: Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction.
abstract: Tapping technique comprises the extraction of pine oleoresin, a non-wood forest product consisting of a complex mixture of mono, sesqui, and diterpenes biosynthesized and exuded as a defense response to wounding. Oleoresin is used to produce gum rosin, turpentine, and their multiple derivatives. Oleoresin yield and quality are objects of interest in pine tree biotechnology, both in terms of environmental and genetic control. Monitoring these parameters in individual trees grown in the field provides a means to examine the control of terpene production in resin canals, as well as the identification of genetic-based differences in resinosis. A typical method of tapping involves the removal of bark and application of a chemical stimulant on the wounded area. Here we describe the methods for preparing the resin-stimulant paste with different adjuvants, as well as the bark streaking process in adult pine trees.
authors: Plant Physiology Laboratory, Center for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, RS, 91501-970, Brazil.|Department of Botany, UFRGS, Porto Alegre, RS, Brazil.
authors: Thanise Nogueira
authors: TN
authors: Füller
authors: Molecular Genetics Laboratory, University of Passo Fundo, Passo Fundo, RS, Brazil.
authors: Júlio César
authors: JC
authors: de Lima
authors: Plant Physiology Laboratory, Department of Botany, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, RS, 91501-970, Brazil.|Center for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors: Fernanda
authors: F
authors: de Costa
authors: Regional Integrated University of Alto Uruguai and Missões (URI-FW), Frederico Westphalen, RS, Brazil.
authors: Kelly C S
authors: KC
authors: Rodrigues-Corrêa
authors: Plant Physiology Laboratory, Center for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, RS, 91501-970, Brazil. fettneto@cbiot.ufrgs.br.|Department of Botany, UFRGS, Porto Alegre, RS, Brazil. fettneto@cbiot.ufrgs.br.
authors: Arthur G
authors: AG
authors: Fett-Neto
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; C018620:oleoresins
keywords: Chemical stimulant; Oleoresin; Pine; Tapping; Wounding