title: Expression of AQP1 Was Associated with Apoptosis and Survival of Patients in Gastric Adenocarcinoma.
abstract: OBJECTIVE
  Recently, interest in the role of aquaporin 1 (AQP1) in human gastrointestinal carcinogenesis has developed. However, to date no studies have examined relationships between AQP1 expression and specific characteristics of gastric adenocarcinoma.
  
  
  METHODS
  We investigated 109 specimens of primary gastric adenocarcinoma and their corresponding normal gastric mucosa using immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) to determine AQP1 expression. We then evaluated disease free survival (DFS) and overall survival (OS) in these patients in association with AQP1 expression.
  
  
  RESULTS
  Both immunohistochemical and RT-PCR analyses identified increased AQP1 expression in tumors from patients with gastric adenocarcinoma (p < 0.001). The 3-year DFS and OS rates were higher in the AQP1-negative group than in the positive group (DFS: 77.2 vs. 52.8%, p < 0.001; OS: 85.1 vs. 70.7%, p < 0.001). The 5-year DFS and OS rates exhibited a similar trend (p < 0.001). Subgroup analysis of patients with early gastric adenocarcinoma (stages I and II) revealed a total 5-year OS of 90.0%, with 5-year OS being higher in the AQP1-negative group than in the positive group (95.2 vs. 84.2%). Furthermore, incidence of tumor recurrence following surgical treatment was significantly higher in the AQP1-positive group (4/19, 21.1%) compared with the negative group (0/21, 0%).
  
  
  CONCLUSIONS
  Our study demonstrates that AQP1 plays an important role in gastric adenocarcinoma and may therefore represent a novel therapeutic target and prognostic marker in this disease.
authors: Department of Surgery, The Second Affiliated Hospital of Wenzhou and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Wei-Jian
authors: WJ
authors: Sun
authors: Dan-Hong
authors: DH
authors: Hu
authors: Hao
authors: H
authors: Wu
authors: Heng
authors: H
authors: Xiao
authors: Ming-Dong
authors: MD
authors: Lu
authors: Wen-Jian
authors: WJ
authors: Guo
authors: He
authors: H
authors: Huang
authors: Yao-Jun
authors: YJ
authors: Yu
authors: Tian-Ye
authors: TY
authors: Hu
authors: Zhi-Qiang
authors: ZQ
authors: Zheng
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D051398:Aquaporin 1 / Q000032:analysis / Q000235:genetics*; D018572:Disease-Free Survival; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics*; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D015996:Survival Rate
chemical_list: C496020:AQP1 protein, human; D051398:Aquaporin 1