title: Biological Activity of Japanese Quince Extract and Its Interactions with Lipids, Erythrocyte Membrane, and Human Albumin.
abstract: The aim of the study was to determine in vitro biological activity of fruit ethanol extract from Chaenomeles speciosa (Sweet) Nakai (Japanese quince, JQ) and its important constituents (-)-epicatechin (EC) and chlorogenic acid (CA). The study also investigated the structural changes in phosphatidylcholine (PC) liposomes, dipalmitoylphosphatidylcholine liposomes, and erythrocyte membranes (RBC) induced by the extract. It was found that the extract effectively inhibits oxidation of RBC, induced by 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), and PC liposomes, induced by UVB radiation and AAPH. Furthermore, JQ extract to a significant degree inhibited the activity of the enzymes COX-1 and COX-2, involved in inflammatory reactions. The extract has more than 2 times greater activity in relation to COX-2 than COX-1 (selectivity ratio 0.48). JQ extract stimulated growth of the beneficial intestinal bacteria Lactobacillus casei and Lactobacillus plantarum. In the fluorimetric method by means of the probes Laurdan, DPH and TMA-DPH, and (1)H-NMR, we examined the structural changes induced by JQ and its EC and CA components. The results show that JQ and its components induce a considerable increase of the packing order of the polar heads of lipids with a slight decrease in mobility of the acyl chains. Lipid membrane rigidification could hinder the diffusion of free radicals, resulting in inhibition of oxidative damage induced by physicochemical agents. JQ extract has the ability to quench the intrinsic fluorescence of human serum albumin through static quenching. This report thus could be of huge significance in the food industry, pharmacology, and clinical medicine.
authors: Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C.K. Norwida 25, 50-375, Wrocław, Poland. paulina.strugala@up.wroc.pl.
authors: Paulina
authors: P
authors: Strugała
authors: Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C.K. Norwida 25, 50-375, Wrocław, Poland.
authors: Sylwia
authors: S
authors: Cyboran-Mikołajczyk
authors: Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C.K. Norwida 25, 50-375, Wrocław, Poland.
authors: Anna
authors: A
authors: Dudra
authors: Department of Fruit, Vegetable and Cereal Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630, Wrocław, Poland.
authors: Paulina
authors: P
authors: Mizgier
authors: Department of Fruit, Vegetable and Cereal Technology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37/41, 51-630, Wrocław, Poland.
authors: Alicja Z
authors: AZ
authors: Kucharska
authors: Department of Chemistry, Wrocław University of Environmental and Life Sciences, C.K. Norwida 25, 50-375, Wrocław, Poland.
authors: Teresa
authors: T
authors: Olejniczak
authors: Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C.K. Norwida 25, 50-375, Wrocław, Poland.
authors: Janina
authors: J
authors: Gabrielska
mesh_terms: D000418:Albumins / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000737:chemistry*; D013058:Mass Spectrometry; D008560:Membrane Fluidity; D010636:Phenols / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D066244:Proton Magnetic Resonance Spectroscopy; D027824:Rosaceae / Q000737:chemistry*; D013552:Swine
chemical_list: D000418:Albumins; D000975:Antioxidants; D016861:Cyclooxygenase Inhibitors; D008055:Lipids; D010636:Phenols; D010713:Phosphatidylcholines; D010936:Plant Extracts
keywords: 1H-NMR and fluorometric study; Erythrocyte and phosphatidylcholine membranes; Human serum albumin; Japanese quince; Lipid peroxidation