title: Development of a serum-free liquid medium for Bartonella species.
abstract: The genus Bartonella comprises numerous species with at least 13 species pathogenic for humans. They are fastidious, aerobic, Gram negative, and facultative intracellular bacteria which cause a variety of human and non-human diseases. This study focused on the development of a serum-free liquid medium for culture of Bartonella species. Some liquid media are available commercially but all of them use undefined supplements such as fetal calf serum or defibrinated sheep blood. Our intention was to create a reproducible liquid medium for Bartonella species that can simply be prepared. We tested several supplements that could potentially support the growth of Bartonella species. Slight growth improvement was achieved with glucose and sucrose. However, hemin in particular improved the growth rate. At a temperature of 37 °C, a CO2 concentration of 5 %, a humidified atmosphere, and the use of the supplements glucose, sucrose, and hemin, we developed a medium that does not need serum as an undefined supplement any more. In conclusion, the newly developed medium supports growth of Bartonella species equal to the commercially available media but with the advantage that it has a serum-free formulation. It can be prepared fast and easy and is a useful tool in studying these bacteria.
authors: Institute for Hygiene and Applied Immunology, Center for Pathophysiology Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria. Andreas.mueller@meduniwien.ac.at.
authors: Andreas
authors: A
authors: Müller
authors: Institute for Hygiene and Applied Immunology, Center for Pathophysiology Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.
authors: Michael
authors: M
authors: Reiter
authors: Institute for Hygiene and Applied Immunology, Center for Pathophysiology Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.
authors: Katrin
authors: K
authors: Mantlik
authors: Institute for Hygiene and Applied Immunology, Center for Pathophysiology Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.
authors: Anna-Margarita
authors: AM
authors: Schötta
authors: Institute for Hygiene and Applied Immunology, Center for Pathophysiology Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.
authors: Hannes
authors: H
authors: Stockinger
authors: Institute for Hygiene and Applied Immunology, Center for Pathophysiology Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090, Vienna, Austria.
authors: Gerold
authors: G
authors: Stanek
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001473:Bartonella / Q000254:growth & development*; D002245:Carbon Dioxide / Q000378:metabolism; D016895:Culture Media, Serum-Free / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006427:Hemin / Q000378:metabolism; D006801:Humans; D013395:Sucrose / Q000378:metabolism; D013696:Temperature
chemical_list: D016895:Culture Media, Serum-Free; D002245:Carbon Dioxide; D013395:Sucrose; D006427:Hemin; D005947:Glucose