title: Altered Th17 Cytokine Expression in Helicobacter pylori Patients with TLR4 (D299G) Polymorphism.
abstract: Helicobacter pylori (H. pylori) is associated with gastric ulcer and gastric adenocarcinoma. Polymorphisms in the host genes coding for Toll-like receptors (TLRs) may influence the innate and adaptive immune response to the infection, affecting the susceptibility to H. pylori or the disease outcomes. However, the details and association with different polymorphism and clinical expression of infection remain unclear. A case-control study consisting of 58 patients with H. pylori infection and 44 H. pylori uninfection was conducted. Genomic DNA was extracted and genotypes of TLR4 Asp299Gly polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Mucosal cytokines expression in H. pylori-infected and uninfected gastric biopsies was determined by real-time PCR. The expression of IL-6, IL-17, IL-21, IL-23 and TGF-β1 was signiﬁcantly higher in patients with D299G polymorphism in TLR4. But the expression of IL-18 between patients with single-nucleotide polymorphisms (SNPs) in TLR4 and patients with the wild-type allele was not signiﬁcant. In H. pylori-infected patients with gastritis, SNPs in TLR4 may alter cytokine expression toward Th17 immune response in the gastric mucosa and may have increased risk for the development of peptic ulcer.
authors: a Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran.
authors: Nader
authors: N
authors: Bagheri
authors: b Cellular and Molecular Research Center , Shahrekord University of Medical Sciences , Shahrekord , Iran.
authors: Fatemeh
authors: F
authors: Azadegan-Dehkordi
authors: c Department of Internal Medicine , Shahrekord University of Medical Sciences , Shahrekord , Iran.
authors: Ghorbanali
authors: G
authors: Rahimian
authors: b Cellular and Molecular Research Center , Shahrekord University of Medical Sciences , Shahrekord , Iran.
authors: Morteza
authors: M
authors: Hashemzadeh-Chaleshtori
authors: d Medical Plants Research Center , Shahrekord University of Medical Sciences , Shahrekord , Iran.
authors: Mahmoud
authors: M
authors: Rafieian-Kopaei
authors: e Biostatistics Department , Shahrekord University of Medical Sciences , Shahrekord , Iran.
authors: Soleyman
authors: S
authors: Kheiri
authors: b Cellular and Molecular Research Center , Shahrekord University of Medical Sciences , Shahrekord , Iran.
authors: Abolfazl
authors: A
authors: Gholipour
authors: b Cellular and Molecular Research Center , Shahrekord University of Medical Sciences , Shahrekord , Iran.
authors: Hedayatollah
authors: H
authors: Shirzad
mesh_terms: D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D001706:Biopsy; D003062:Codon; D016207:Cytokines / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D055815:Young Adult
chemical_list: D003062:Codon; D016207:Cytokines; D051197:Toll-Like Receptor 4
keywords: Cytokine; Helicobacter pylori; TLR4; Th17; gastritis; gene polymorphism