title: Microalgae from domestic wastewater facility's high rate algal pond: Lipids extraction, characterization and biodiesel production.
abstract: In this study, the harvesting of a biomass from a high rate algal pond (HRAP) of a real-scale domestic wastewater treatment facility and its potential as a biomaterial for the production of biodiesel were investigated. Increasing the medium pH to 12 induced high flocculation efficiency of up to 96% of the biomass through both sweep flocculation and charge neutralization. Lipids extracted by ultrasounds from this biomass contained around 70% of fatty acids, with palmitic and stearic acids being the most abundant. The extract obtained by supercritical CO2 contained 86% of fatty acids. Both conventional solvents extracts contained only around 10% of unsaturated fats, whereas supercritical CO2 extract contained more than 40% of unsaturated fatty acids. This same biomass was also subject to direct extractive-transesterification in a microwave reactor to produce fatty acid methyl esters, also known as, raw biodiesel.
authors: Université de Monastir, Faculté des Sciences, Département de Chimie, UR13ES63-Chimie Appliquée et Environnement, Bvd de l'Environnement, 5000 Monastir, Tunisia.
authors: Neila
authors: N
authors: Drira
authors: Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, SP 8 Monserrato - Sestu km 0.700, 09042 Monserrato, CA, Italy.
authors: Alessandra
authors: A
authors: Piras
authors: Dipartimento di Scienze Biomediche, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, SP 8 Monserrato - Sestu km 0.700, 09042 Monserrato, CA, Italy.
authors: Antonella
authors: A
authors: Rosa
authors: Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella Universitaria di Monserrato, SP 8 Monserrato - Sestu km 0.700, 09042 Monserrato, CA, Italy.
authors: Silvia
authors: S
authors: Porcedda
authors: Université de Monastir, Faculté des Sciences, Département de Chimie, UR13ES63-Chimie Appliquée et Environnement, Bvd de l'Environnement, 5000 Monastir, Tunisia. Electronic address: hatem.dhaouadi@fsm.rnu.tn.
authors: Hatem
authors: H
authors: Dhaouadi
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002118:Calcium / Q000032:analysis; D004553:Electric Conductivity; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000302:isolation & purification*; D008274:Magnesium / Q000032:analysis; D058086:Microalgae / Q000378:metabolism* / Q000648:ultrastructure; D008872:Microwaves; D061005:Ponds / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology*
chemical_list: D056804:Biofuels; D002118:Calcium; D005227:Fatty Acids; D008055:Lipids; D008274:Magnesium; D062065:Wastewater
keywords: Biodiesel; Biomass flocculation/coagulation; Lipid extraction; Wastewater