title: Co-delivery of VEGF and bFGF via a PLGA nanoparticle-modified BAM for effective contracture inhibition of regenerated bladder tissue in rabbits.
abstract: Graft contracture is a common problem associated with the regeneration processes of tissue-engineered bladders. Currently, most strategies used for incorporating bioactive molecules into biomaterial designs do not work during all phases of tissue regeneration. In this study, we used a growth factor-PLGA nanoparticle thermo-sensitive gel system (i.e., BAM with incorporated VEGF and bFGF-loaded PLGA nanoparticles and mixed with a hydrophilic gel) to promote bladder tissue regeneration in a rabbit model. At 4 and 12 weeks after surgery, contracture rate assessment and histological examination were conducted to evaluate bladder tissue regeneration. The results indicated that the functional composite scaffold continuously and effectively released VEGF and bFGF and promoted bladder reconstruction with a significant decrease in graft contracture. In addition, the number and arrangement of regenerated urothelial cells and smooth muscle cells as well as microvascular density and maturity were improved in the VEGF/bFGF nanoparticle group compared with the single factor VEGF or bFGF nanoparticle group and BAM alone. The nanoparticle thermo-sensitive gel system, which exhibited favourable performance, may effectively inhibit graft contracture and promote bladder tissue regeneration in rabbits.
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Xincheng
authors: X
authors: Jiang
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Houwei
authors: H
authors: Lin
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Dapeng
authors: D
authors: Jiang
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Guofeng
authors: G
authors: Xu
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Xiaoliang
authors: X
authors: Fang
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Lei
authors: L
authors: He
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Maosheng
authors: M
authors: Xu
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Bingqiang
authors: B
authors: Tang
authors: Shanghai Institute of Pharmaceutical Industry, Shanghai, China.
authors: Zhiyong
authors: Z
authors: Wang
authors: Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Department of Bio-Nano Science and Engineering, Institute of Micro-Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, China.
authors: Daxiang
authors: D
authors: Cui
authors: Department of Urology, Children's Hospital affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Fang
authors: F
authors: Chen
authors: Department of Pediatric Urology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Hongquan
authors: H
authors: Geng
mesh_terms: D000368:Aged; D000818:Animals; D003286:Contracture / Q000517:prevention & control*; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage*; D008297:Male; D023421:Models, Animal; D053758:Nanoparticles / Q000008:administration & dosage*; D009842:Oligopeptides / Q000008:administration & dosage*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D016896:Treatment Outcome; D001743:Urinary Bladder / Q000187:drug effects*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage*
chemical_list: D009842:Oligopeptides; D042461:Vascular Endothelial Growth Factor A; C588228:lysyl-arginyl-threonyl-glycyl-glutaminyl-tyrosyl-lysyl-leucyl-cysteine; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid