title: Inhibiting malignant phenotypes of the bladder cancer cells by silencing long noncoding RNA SChLAP1.
abstract: OBJECTIVE
  Long noncoding RNAs (lncRNAs) play key roles in process of cancer cell growth and apoptosis and have received increasing attention. SChLAP1 is a novel lncRNA that is required for development and progression of prostate cancer. We hypothesized that SChLAP1 also has important biological functions in human bladder cancer which is another type of urological cancer.
  
  
  METHODS
  The expression of SChLAP1 in bladder cancer was determined using real-time qPCR. Bladder cancer T24 and 5637 cells were transfected with SChLAP1 siRNA or negative control siRNA. Cell proliferation, apoptosis and migration were determined using CCK-8 assay, flow cytometry analysis and wound healing assay, respectively.
  
  
  RESULTS
  SChLAP1 was overexpressed in bladder cancer tissues compared to paired normal bladder tissues. Cell growth arrest, apoptosis induction and migration inhibition were also observed in bladder cancer T24 and 5637 cells after transfection with SChLAP1 siRNA.
  
  
  CONCLUSIONS
  Our data suggest that SChLAP1 plays oncogenic roles and can be used as a therapeutic target for treating human bladder cancer.
authors: Department of Urology Surgery Center, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830002, Xinjiang, People's Republic of China.
authors: Jianjun
authors: J
authors: Zhang
authors: Department of Urology Surgery Center, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830002, Xinjiang, People's Republic of China.
authors: Zhenfeng
authors: Z
authors: Shi
authors: Department of Urology Surgery Center, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830002, Xinjiang, People's Republic of China.
authors: Yukui
authors: Y
authors: Nan
authors: Department of Urology Surgery Center, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, 830002, Xinjiang, People's Republic of China. mingli23455@sina.com.
authors: Ming
authors: M
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000032:analysis* / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C000613525:SChLAP1 long noncoding RNA
keywords: Bladder cancer; Cell apoptosis; Cell migration; Cell proliferation; SChLAP1