title: [Expression of miRNA-4497 in human chorionic villi from early recurrent miscarriage and the influence on apoptosis].
abstract: OBJECTIVE
  To investigate the expression of miRNA-4497 in human chorionic villi from early recurrent miscarriage and the influence on apoptosis.
  
  
  METHODS
  The expression of miRNA-4497 in chorionic villi tissues collected from 20 early recurrent miscarriage cases and 20 controls were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). Human BeWo trophoblastic strains transfected with miRNA-4497 mimic were collected before transfection and 24 h later to measure apoptosis by flow cytometry.
  
  
  RESULTS
  miRNA-4497 was up-regulated in early recurrent miscarriage (24.5% ± 5.5% vs 1.1% ± 0.1%, P<0.05), and there was significantly increase in apoptosis after miRNA-4497 mimic transfection (12.2% ± 2.2% vs 7.3% ± 1.2%, P<0.05).
  
  
  CONCLUSIONS
  Up-regulation of miR-4497 may play an important role in the pathogenesis of recurrent miscarriage. And the pathophysiological mechanism involved should be further studied.
authors: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors: Lisha
authors: L
authors: Tang
authors: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors: Chao
authors: C
authors: Gao
authors: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors: Li
authors: L
authors: Gao
authors: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors: Yugui
authors: Y
authors: Cui
authors: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors: Jiahao
authors: J
authors: Sha
authors: State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China; Email: jyliu_nj@126.com.
authors: Jiayin
authors: J
authors: Liu
mesh_terms: D000026:Abortion, Habitual; D017209:Apoptosis; D002824:Chorionic Villi; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D035683:MicroRNAs; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D014327:Trophoblasts; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs