title: Intranasal Delivery of Recombinant NT4-NAP/AAV Exerts Potential Antidepressant Effect.
abstract: The present study was designed to construct a recombinant adeno-associated virus (rAAV) which can express NAP in the brain and examine whether this virus can produce antidepressant effects on C57 BL/6 mice that had been subjected to open field test and forced swimming test, via nose-to-brain pathway. When the recombinant plasmid pGEM-T Easy/NT4-NAP was digested by EcoRI, 297 bp fragments can be obtained and NT4-NAP sequence was consistent with the designed sequence confirmed by DNA sequencing. When the recombinant plasmid pSSCMV/NT4-NAP was digested by EcoRI, 297 bp fragments is visible. Immunohistochemical staining of fibroblasts revealed that expression of NAP was detected in NT4-NAP/AAV group. Intranasal delivery of NT4-NAP/AAV significantly reduced immobility time when the FST was performed after 1 day from the last administration. The effects observed in the FST could not be attributed to non-specific increases in activity since intranasal delivery of NT4-NAP/AAV did not alter the behavior of the mice during the open field test. The results indicated that a recombinant AAV vector which could express NAP in cells was successfully constructed and NAP may be a potential target for therapeutic action of antidepressant treatment.
authors: Department of Psychiatry, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China.
authors: Xian-Cang
authors: XC
authors: Ma
authors: College of Medicine and Forensics, Xi'an Jiaotong University Health Science Center, Xi'an, China.
authors: Zheng
authors: Z
authors: Chu
authors: Department of CT/MRI, Shaanxi Provincial People's Hospital, Xi'an, China.
authors: Xiao-Ling
authors: XL
authors: Zhang
authors: Department of Psychiatry, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China.
authors: Wen-Hui
authors: WH
authors: Jiang
authors: Department of Psychiatry, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China.
authors: Min
authors: M
authors: Jia
authors: College of Medicine and Forensics, Xi'an Jiaotong University Health Science Center, Xi'an, China. psydyh@mail.xjtu.edu.cn.|Key Laboratory of the Health Ministry for Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, China. psydyh@mail.xjtu.edu.cn.|Key Laboratory of Environment and Genes Related to Diseases of the Education Ministry, Xi'an Jiaotong University Health Science Center, Xi'an, China. psydyh@mail.xjtu.edu.cn.
authors: Yong-Hui
authors: YH
authors: Dang
authors: Department of Psychiatry, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, China. yaogaow@163.com.
authors: Cheng-Ge
authors: CG
authors: Gao
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage*; D001483:Base Sequence; D000229:Dependovirus / Q000235:genetics; D003863:Depression / Q000188:drug therapy / Q000235:genetics / Q000523:psychology; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000008:administration & dosage / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000008:administration & dosage* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000008:administration & dosage / Q000235:genetics; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics; D016896:Treatment Outcome
chemical_list: C117632:Adnp protein, mouse; D000928:Antidepressive Agents; D018398:Homeodomain Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C073922:neurotrophin 4
keywords: Depression; FST; Intranasal administration; NAP; NT4-NAP/AAV; OFT