title: iTRAQ-Based Quantitative Proteomic Analysis of Cotton Roots and Leaves Reveals Pathways Associated with Salt Stress.
abstract: Salinity is a major abiotic stress that affects plant growth and development. In this study, we performed a proteomic analysis of cotton roots and leaf tissue following exposure to saline stress. 611 and 1477 proteins were differentially expressed in the roots and leaves, respectively. In the roots, 259 (42%) proteins were up-regulated and 352 (58%) were down-regulated. In the leaves, 748 (51%) proteins were up-regulated and 729 (49%) were down-regulated. On the basis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, we concluded that the phenylalanine metabolism and starch and sucrose metabolism were active for energy homeostasis to cope with salt stress in cotton roots. Moreover, photosynthesis, pyruvate metabolism, glycolysis / gluconeogenesis, carbon fixation in photosynthetic organisms and phenylalanine metabolism were inhabited to reduce energy consumption. Characterization of the signaling pathways will help elucidate the mechanism activated by cotton in response to salt stress.
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Tingting
authors: T
authors: Chen
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Lei
authors: L
authors: Zhang
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Haihong
authors: H
authors: Shang
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Shaodong
authors: S
authors: Liu
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Jun
authors: J
authors: Peng
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Wankui
authors: W
authors: Gong
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Yuzhen
authors: Y
authors: Shi
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Siping
authors: S
authors: Zhang
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Junwen
authors: J
authors: Li
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Juwu
authors: J
authors: Gong
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Qun
authors: Q
authors: Ge
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Aiying
authors: A
authors: Liu
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Huijuan
authors: H
authors: Ma
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Xinhua
authors: X
authors: Zhao
authors: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000, P. R. China.
authors: Youlu
authors: Y
authors: Yuan
mesh_terms: D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics / Q000378:metabolism*; D009997:Osmotic Pressure; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D010940:Plant Proteins