title: The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases.
abstract: Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mRNA. We show that the 3'UTR of the RTR1 mRNA contains a cis element that destabilizes this mRNA. RTR1 mRNA turnover is achieved through binding of Rtr1p to the RTR1 mRNP in a manner that is dependent on this cis element. Genetic evidence shows that Rtr1p-mediated decay of the RTR1 mRNA involves the 5'-3' DExD/H-box RNA helicase Dhh1p and the 3'-5' exonucleases Rex2p and Rex3p. Rtr1p and Rex3p are found associated with Dhh1p, suggesting a model for recruiting the REX exonucleases to the RTR1 mRNA for degradation. Rtr1p-mediated decay potentially impacts additional transcripts, including the unspliced BMH2 pre-mRNA. We propose that Rtr1p may imprint its RNA targets cotranscriptionally and determine their downstream degradation mechanism by directing these transcripts to a novel turnover pathway that involves Rtr1p, Dhh1p, and the REX family of exonucleases.
authors: Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA.
authors: Domagoj
authors: D
authors: Hodko
authors: Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA.
authors: Taylor
authors: T
authors: Ward
authors: Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, USA Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, USA.
authors: Guillaume
authors: G
authors: Chanfreau
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D005095:Exoribonucleases / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger; C530135:Rtr1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005095:Exoribonucleases; C000609575:Rex2 protein, S cerevisiae; C000609574:Rex3 protein, S cerevisiae
keywords: REX; RNA; RTR1; autoregulation; degradation; yeast