title: Metabolic Engineering of Pseudomonas putida KT2440 for Complete Mineralization of Methyl Parathion and γ-Hexachlorocyclohexane.
abstract: Agricultural soils are often cocontaminated with multiple pesticides. Unfortunately, microorganisms isolated from natural environments do not possess the ability to simultaneously degrade different classes of pesticides. Currently, we can use the approaches of synthetic biology to create a strain endowed with various catabolic pathways that do not exist in a natural microorganism. Here, we describe the metabolic engineering of a biosafety Pseudomonas putida strain KT2440 for complete mineralization of methyl parathion (MP) and γ-hexachlorocyclohexane (γ-HCH) by functional assembly of the MP and γ-HCH mineralization pathways. The engineered strain was genetically stable, and no growth inhibition was observed. Such a strain not only would reduce the toxicity of MP and γ-HCH but also would prevent the accumulation of potentially toxic intermediates in the environment. Furthermore, expression of Vitreoscilla hemoglobin improved the ability of the engineered strain to sequester O2. The inoculation of the engineered strain to soils treated with MP and γ-HCH resulted in a higher degradation rate than in noninoculated soils. Moreover, introduced GFP may be used to monitor the activity of the engineered strain during bioremediation. The engineered strain may be a promising candidate for in situ bioremediation of soil cocontaminated with MP and γ-HCH.
authors: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China.
authors: Ting
authors: T
authors: Gong
authors: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China.
authors: Ruihua
authors: R
authors: Liu
authors: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China.
authors: Zhenqiang
authors: Z
authors: Zuo
authors: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China.
authors: You
authors: Y
authors: Che
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai 200237, China.
authors: Huilei
authors: H
authors: Yu
authors: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China.
authors: Cunjiang
authors: C
authors: Song
authors: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education and State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China.
authors: Chao
authors: C
authors: Yang
mesh_terms: D001673:Biodegradation, Environmental; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods; D008660:Metabolism / Q000235:genetics; D008743:Methyl Parathion / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism*; D058615:Synthetic Biology / Q000379:methods
chemical_list: D008743:Methyl Parathion; D001556:Hexachlorocyclohexane; D010100:Oxygen
keywords: Pseudomonas putida KT2440; complete mineralization; methyl parathion; γ-hexachlorocyclohexane