title: Pathogenicity of a currently circulating Chinese variant pseudorabies virus in pigs.
abstract: OBJECTIVE
  To test the pathogenicity of pseudorabies virus (PRV) variant HN1201 and compare its pathogenicity with a classical PRV Fa strain.
  
  
  METHODS
  The pathogenicity of the newly-emerging PRV variant HN1201 was evaluated by different inoculating routes, virus loads, and ages of pigs. The classical PRV Fa strain was then used to compare with HN1201 to determine pathogenicity. Clinical symptoms after virus infection were recorded daily and average daily body weight was used to measure the growth performance of pigs. At necropsy, gross pathology and histopathology were used to evaluate the severity of tissue damage caused by virus infection.
  
  
  RESULTS
  The results showed that the efficient infection method of RPV HN1201 was via intranasal inoculation at 10(7) TCID50, and that the virus has high pathogenicity to 35- to 127-d old pigs. Compared with Fa strain, pigs infected with HN1201 showed more severe clinical symptoms and pathological lesions. Immunochemistry results revealed HN1201 had more abundant antigen distribution in extensive organs.
  
  
  CONCLUSIONS
  All of the above results suggest that PRV variant HN1201 was more pathogenic to pigs than the classical Fa strain.
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Qing-Yuan
authors: QY
authors: Yang
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Zhe
authors: Z
authors: Sun
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Fei-Fei
authors: FF
authors: Tan
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Ling-Hua
authors: LH
authors: Guo
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Yu-Zhou
authors: YZ
authors: Wang
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Juan
authors: J
authors: Wang
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Zhi-Yan
authors: ZY
authors: Wang
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Li-Lin
authors: LL
authors: Wang
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Xiang-Dong
authors: XD
authors: Li
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Yan
authors: Y
authors: Xiao
authors: Qing-Yuan Yang, Zhe Sun, Fei-Fei Tan, Ling-Hua Guo, Yu-Zhou Wang, Juan Wang, Zhi-Yan Wang, Li-Lin Wang, Xiang-Dong Li, Yan Xiao, Ke-Gong Tian, National Research Center for Veterinary Medicine, Luoyang 471003, Henan Province, China.
authors: Ke-Gong
authors: KG
authors: Tian
keywords: Gross pathology; Histopathology; Pathogenicity; Pseudorabies virus; Virus variant