title: Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves.
abstract: The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.
authors: DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark.
authors: Svend Roesen
authors: SR
authors: Madsen
authors: DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark.
authors: Hussam Hassan
authors: HH
authors: Nour-Eldin
authors: DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark. bah@plen.ku.dk.
authors: Barbara Ann
authors: BA
authors: Halkier
mesh_terms: D000772:Anesthesia, Local; D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D005961:Glucosinolates / Q000737:chemistry / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism*; D014618:Vacuum
chemical_list: D005961:Glucosinolates
keywords: Apoplastic fluid; Glucosinolates; Guttation fluid; Vacuum infiltration