title: Nondestructive Total Excitation-Emission Fluorescence Microscopy Combined with Multi-Way Chemometric Analysis for Visually Indistinguishable Single Fiber Discrimination.
abstract: The potential of total excitation-emission fluorescence microscopy combined with multiway chemometric analysis was investigated for the nondestructive forensic analysis of textile fibers. The four pairs of visually indistinguishable fibers consisted of nylon 361 dyed with acid yellow 17 and acid yellow 23, acetate satin 105B dyed with disperse blue 3 and disperse blue 14, polyester 777 dyed with disperse red 1 and disperse red 19, and acrylic 864 dyed with basic green 1 and basic green 4. Excitation emission matrices were recorded with the aid of an inverted microscope and a commercial spectrofluorimeter. The full information content of excitation-emission matrices was processed with the aid of unsupervised parallel factor analysis (PARAFAC), PARAFAC supervised by linear discriminant analysis (LDA), and discriminant unfolded partial least-squares (DU-PLS). The ability of the latter algorithm to classify the four pairs of fibers demonstrates the advantage of using the multidimensionality of fluorescence data formats for the nondestructive analysis of forensic fiber evidence.
authors: Department of Chemistry, University of Central Florida , 4111 Libra Drive, P.O. Box 25000, Orlando, Florida 32816-2366, United States.|Department of Analytical Chemistry and IACYS, University of Extremadura , Badajoz, 06006, Spain.
authors: Arsenio
authors: A
authors: Muñoz de la Peña
authors: Department of Chemistry, University of Central Florida , 4111 Libra Drive, P.O. Box 25000, Orlando, Florida 32816-2366, United States.
authors: Nirvani
authors: N
authors: Mujumdar
authors: Department of Chemistry, University of Central Florida , 4111 Libra Drive, P.O. Box 25000, Orlando, Florida 32816-2366, United States.
authors: Emily C
authors: EC
authors: Heider
authors: Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional de Litoral , Santa Fe S3000ZAA, Argentina.
authors: Hector C
authors: HC
authors: Goicoechea
authors: Department of Automation and Systems Engineering, University of Seville , Seville, 41092, Spain.
authors: David
authors: D
authors: Muñoz de la Peña
authors: Department of Chemistry, University of Central Florida , 4111 Libra Drive, P.O. Box 25000, Orlando, Florida 32816-2366, United States.|National Center for Forensic Science, University of Central Florida , 12354 Research Parkway, Suite 225, Orlando, Florida 32826, United States.
authors: Andres D
authors: AD
authors: Campiglia
mesh_terms: D008856:Microscopy, Fluorescence / Q000379:methods*