title: Calcium homeostasis in mitochondrion-mediated apoptosis of chick embryo cecal epithelial cells induced by Eimeria tenella infection.
abstract: In this study, the process of Eimeria tenella-induced apoptosis and the effect of calcium homeostasis were investigated in chick embryo cecal epithelial cells. In particular, we examined cytochrome c release into the cytoplasm, mitochondrial permeability transition pore (MPTP) opening, and changes in [Ca(2+)]c and apoptosis in host cells. Apoptosis, MPTP opening, cytochrome c release, and [Ca(2+)]c in host cells increased following infection. This trend was reversed by blocking the increase in [Ca(2+)]c using BAPTA/AM and EGTA (intra- and extracellular chelators of Ca(2+), respectively) and by applying heparin sodium and ryanodine (blockers of the inositol triphosphate and ryanodine receptors of the endoplasmic reticulum, respectively). These results indicate that [Ca(2+)]c plays a significant role in host cell mitochondrial apoptosis, which is induced via modulation of extracellular Ca(2+) levels and endoplasmic reticulum Ca(2+) channels. Thus, agents that restore Ca(2+) homeostasis may be useful for managing E. tenella infection in chickens.
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Xiao-zhen
authors: XZ
authors: Cui
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China. Electronic address: zhengmingxue288@163.com.
authors: Ming-xue
authors: MX
authors: Zheng
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Yan
authors: Y
authors: Zhang
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Rui-li
authors: RL
authors: Liu
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Sha-sha
authors: SS
authors: Yang
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Shan
authors: S
authors: Li
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Zhi-yong
authors: ZY
authors: Xu
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Rui
authors: R
authors: Bai
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Qiang-hua
authors: QH
authors: Lv
authors: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China.
authors: Wen-long
authors: WL
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism*; D002432:Cecum / Q000502:physiology; D002642:Chick Embryo; D002645:Chickens; D003048:Coccidiosis / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D016786:Eimeria tenella / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology; D006706:Homeostasis; D008928:Mitochondria / Q000502:physiology; D011201:Poultry Diseases / Q000378:metabolism* / Q000469:parasitology
chemical_list: D002118:Calcium
keywords: Apoptosis; Cytochrome c; Cytosolic calcium; Eimeria tenella; Mitochondrial