title: Changing expression profiles of lncRNAs, mRNAs, circRNAs and miRNAs during osteoclastogenesis.
abstract: Bone is a dynamic organ continuously undergoing shaping, repairing and remodeling. The homeostasis of bone is maintained by the balance between osteoblastic bone formation and osteoclastic bone resorption. Osteoclasts (OCs) are specialized multinucleated cells derived from hematopoietic stem cells (HSCs) or monocytes/macrophage progenitor cells. There are different stages during osteoclastogenesis, and one of the most important steps to form functional osteoclasts is realized by cell-cell fusion. In our study, microarray was performed to detect the expression profiles of lncRNA, mRNA, circRNA and miRNA at different stages during osteoclastogenesis of RAW264.7 cells. Often changed RNAs were selected and clustered among the four groups with Venn analysis. The results revealed that expressions of 518 lncRNAs, 207 mRNAs, 24 circRNAs and 37 miRNAs were often altered at each stage during OC differentiation. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analysis were performed to predict the functions of differentially expressed lncRNAs and co-expressed potential targeting genes. Co-expression networks of lncRNA-mRNA and circRNA-miRNA were constructed based on the correlation analysis between the differentially expressed RNAs. The present study provided a systematic perspective on the potential function of non-coding RNAs (ncRNAs) during osteoclastogenesis.
authors: Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing, China.|Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Ce
authors: C
authors: Dou
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Zhen
authors: Z
authors: Cao
authors: Department of Anatomy, Third Military Medical University, Chongqing, China.
authors: Bo
authors: B
authors: Yang
authors: Department of Anatomy, Third Military Medical University, Chongqing, China.
authors: Ning
authors: N
authors: Ding
authors: Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Tianyong
authors: T
authors: Hou
authors: Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Fei
authors: F
authors: Luo
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Fei
authors: F
authors: Kang
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Jianmei
authors: J
authors: Li
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Xiaochao
authors: X
authors: Yang
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Hong
authors: H
authors: Jiang
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Junyu
authors: J
authors: Xiang
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.
authors: Hongyu
authors: H
authors: Quan
authors: Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors: Jianzhong
authors: J
authors: Xu
authors: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, China.|China Orthopedic Regenerative Medicine Group (CORMed), Chongqing, China.
authors: Shiwu
authors: S
authors: Dong
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger