title: Colorimetric detection of hepatitis B virus (HBV) DNA based on DNA-templated copper nanoclusters.
abstract: DNA detection plays an important role in early diagnosis of genetic disease. The conventional detection methods of DNA are based on expensive equipment, which do not meet the demands of developing countries. Thus, we developed a colorimetric method, which could be observed with naked eye and used copper nanoclusters for cost-effective. Moreover, the target of this method is the DNA in Hepatitis B virus that is one of the most popular chronic viral infections in developing countries over the past years. Our method was sensitive and the limit of detection was 12 × 10(9) molecules. Three-base-pair mismatches target DNA was detected easily. These results revealed the favorable sensitivity and selectivity of this approach. Most importantly, our method may have potential applications in correct diagnosis of genetic disease and monitoring of gene therapy in the poverty-stricken areas.
authors: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; Department of Life Science, Anqing Normal University, Anqing, Anhui 246011, China.
authors: Xiaoxia
authors: X
authors: Mao
authors: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors: Siyu
authors: S
authors: Liu
authors: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors: Chao
authors: C
authors: Yang
authors: Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China.
authors: Fengzhen
authors: F
authors: Liu
authors: Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China. Electronic address: wkmys@sohu.com.
authors: Keming
authors: K
authors: Wang
authors: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address: gfchen@shu.edu.cn.
authors: Guifang
authors: G
authors: Chen
mesh_terms: D003124:Colorimetry; D003300:Copper / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics* / Q000302:isolation & purification; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D004279:DNA, Viral; D003300:Copper
keywords: Biosensor; Catalysis; Colorimetry; Copper nanoclusters; DNA; Hepatitis B virus