title: Spectrum-Effect Relationships Between Chemical Fingerprints and Antibacterial Effects of Lonicerae Japonicae Flos and Lonicerae Flos Base on UPLC and Microcalorimetry.
abstract: The traditional Chinese medicines Lonicerae Japonicae Flos (LJF, Jinyinhua in Chinese) and Lonicerae Flos (LF, Shanyinhua in Chinese) refer to the flower buds of five plants belonging to the Caprifoliaceae family. Until 2000, all of these were officially listed as a single item, LJF (Jinyinhua), in the Chinese Pharmacopoeia. However, there have recently been many academic controversies concerning the separation and combination of LJF and LF in administrative regulation. Till now there has been little work completed evaluating the relationships between biological activity and chemical properties among these drugs. Microcalorimetry and UPLC were used along with principal component analysis (PCA), hierarchical cluster analysis (HCA), and canonical correlation analysis (CCA) to investigate the relationships between the chemical ingredients and the antibacterial effects of LJF and LF. Using multivariate statistical analysis, LJF and LF could be initially separated according to their chemical fingerprints, and the antibacterial effects of the two herbal drugs were divided into two clusters. This result supports the disaggregation of LJF and LF by the Pharmacopoeia Committee. However, the sample of Lonicera fulvotomentosa Hsu et S. C. Cheng turned out to be an intermediate species, with similar antibacterial efficacy as LJF. The results of CCA indicated that chlorogenic acid and 3,4-Dicaffeoylquinic acid were the major components generating antibacterial effects. Furthermore, 3,4-Dicaffeoylquinic acid could be used as a new marker ingredient for quality control of LJF and LF.
authors: Pharmacy College, Chengdu University of Traditional Chinese MedicineChengdu, China; China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation ArmyBeijing, China.
authors: Zhilong
authors: Z
authors: Shi
authors: Pharmacy College, Chengdu University of Traditional Chinese MedicineChengdu, China; China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation ArmyBeijing, China.
authors: Zhenjie
authors: Z
authors: Liu
authors: School of Chinese Pharmacy, Beijing University of Chinese Medicine Beijing, China.
authors: Chunsheng
authors: C
authors: Liu
authors: Pharmacy College, Chengdu University of Traditional Chinese MedicineChengdu, China; China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation ArmyBeijing, China.
authors: Mingquan
authors: M
authors: Wu
authors: Liver Failure Therapy and Research Center, 302 Hospital of People's Liberation Army Beijing, China.
authors: Haibin
authors: H
authors: Su
authors: Pharmacy College, Chengdu University of Traditional Chinese MedicineChengdu, China; China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation ArmyBeijing, China.
authors: Xiao
authors: X
authors: Ma
authors: School of Chinese Pharmacy, Beijing University of Chinese Medicine Beijing, China.
authors: Yimei
authors: Y
authors: Zang
authors: China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation Army Beijing, China.
authors: Jiabo
authors: J
authors: Wang
authors: China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation Army Beijing, China.
authors: Yanling
authors: Y
authors: Zhao
authors: China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation Army Beijing, China.
authors: Xiaohe
authors: X
authors: Xiao
keywords: Lonicerae Japonicae Flos; UPLC; antibacterial; microcalorimetry; spectrum-effect relationships