title: Sexual differentiation and reproductive development of female rat offspring after paternal exposure to the anti-tumor pharmaceutical cisplatin.
abstract: Cisplatin (CP) is used to treat a number of cancers, including testicular cancer. Studies indicate that CP-treatment can impair spermatogenesis in humans and rodents by germ cell DNA binding, through different modes of action. CP-paternal exposure resulted in adverse effects in F1 male offspring. In this study, F1 female offspring was assessed for reproductive development after CP-paternal exposure. Peri-pubertal male rats, treated with 1mg/Kg/day of CP or vehicle for 3 weeks, were mated with unexposed females. F1 female offspring of CP-treated fathers showed a decrease in fetal ovary germ cells, in estrous cycle length and FSH levels, and an increase in the percentage of antral follicles in adults. Based on our previous results and the findings of the present work we concluded that CP-paternal exposure leads to adverse effects on rat male and female reproductive development, raising concern, in humans, for children born to men exposed to CP.
authors: Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors: Patrícia Villela
authors: PV
authors: E Silva
authors: Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors: Raquel Frenedoso
authors: RF
authors: da Silva
authors: Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors: Cibele Dos Santos
authors: Cdos S
authors: Borges
authors: Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors: Marilia Martins
authors: MM
authors: Cavariani
authors: Department of Anatomy, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors: Camila Contin Diniz
authors: CC
authors: de Almeida Francia
authors: Department of Clinical, Toxicological and Bromatological Analyses, Faculty of Pharmaceutical Sciences of Ribeirão Preto, USP-University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Fernando Barbosa
authors: FB
authors: Júnior
authors: Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil. Electronic address: kempinas@ibb.unesp.br.
authors: Wilma
authors: W
authors: De Grava Kempinas
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000097:blood; D005854:Germ Cells / Q000187:drug effects; D008297:Male; D010053:Ovary / Q000187:drug effects; D018812:Paternal Exposure; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D017208:Rats, Wistar; D012733:Sex Differentiation / Q000187:drug effects*; D012726:Sexual Behavior, Animal / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D005640:Follicle Stimulating Hormone; D002945:Cisplatin
keywords: Cisplatin; Female rat; Paternal exposure; Reproductive toxicity