title: Nucleolar DEAD-Box RNA Helicase TOGR1 Regulates Thermotolerant Growth as a Pre-rRNA Chaperone in Rice.
abstract: Plants have evolved a considerable number of intrinsic tolerance strategies to acclimate to ambient temperature increase. However, their molecular mechanisms remain largely obscure. Here we report a DEAD-box RNA helicase, TOGR1 (Thermotolerant Growth Required1), prerequisite for rice growth themotolerance. Regulated by both temperature and the circadian clock, its expression is tightly coupled to daily temperature fluctuations and its helicase activities directly promoted by temperature increase. Located in the nucleolus and associated with the small subunit (SSU) pre-rRNA processome, TOGR1 maintains a normal rRNA homeostasis at high temperature. Natural variation in its transcript level is positively correlated with plant height and its overexpression significantly improves rice growth under hot conditions. Our findings reveal a novel molecular mechanism of RNA helicase as a key chaperone for rRNA homeostasis required for rice thermotolerant growth and provide a potential strategy to breed heat-tolerant crops by modulating the expression of TOGR1 and its orthologs.
authors: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing, China.
authors: Dong
authors: D
authors: Wang
authors: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing, China.
authors: Baoxiang
authors: B
authors: Qin
authors: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing, China.
authors: Xiang
authors: X
authors: Li
authors: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing, China.
authors: Ding
authors: D
authors: Tang
authors: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing, China.
authors: Yu'e
authors: Y
authors: Zhang
authors: State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing, China.|Collaborative Innovation Center for Genetics and Development, Fudan University, Shanghai, China.
authors: Zhukuan
authors: Z
authors: Cheng
authors: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing, China.|Collaborative Innovation Center for Genetics and Development, Fudan University, Shanghai, China.|Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
authors: Yongbiao
authors: Y
authors: Xue
mesh_terms: D000222:Adaptation, Physiological; D002466:Cell Nucleolus / Q000201:enzymology*; D049109:Cell Proliferation; D002940:Circadian Rhythm / Q000235:genetics; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D063245:Plant Development; D010940:Plant Proteins / Q000378:metabolism*; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D054679:Ribosome Subunits, Small / Q000378:metabolism; D013696:Temperature
chemical_list: D010940:Plant Proteins; D012322:RNA Precursors; D053487:DEAD-box RNA Helicases