title: Control of Grifola frondosa Morphology by Agitation and Aeration for Improving Mycelia and Exo-Polymer Production.
abstract: The present study describes the improved mycelia and exo-polymer production under control of Grifola frondosa morphology by changing the aeration rate and agitation intensity in a 25-L stirred fermentor. The aeration rate of 1.0 vvm yielded a highest mycelia biomass of 24.754 g/L with the lowest pellet percentage of 20.5 %. The maximum exo-polymer (2.324 g/L) was achieved at 0.75 vvm with mycelia polysaccharide production (0.321 g/g), whereas clumps and filaments dominated the ratios of 45.6 and 33.9 %, respectively. The change of aeration rate and agitation intensity had slight influence on the monosaccharide compositions in exo-polymers and significantly affected glucose and mannose contents in the mycelia polysaccharides. These findings will provide a clue for exploring the relationship between fermentation parameters, morphologies, and polysaccharide synthesis pathway of G. frondosa.
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China. fengjiecui@163.com.|Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing, 334221, People's Republic of China. fengjiecui@163.com.
authors: Feng-Jie
authors: FJ
authors: Cui
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
authors: Xiao-Xiao
authors: XX
authors: Chen
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
authors: Wei-Min
authors: WM
authors: Liu
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China. sunwenjing1919@163.com.|Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing, 334221, People's Republic of China. sunwenjing1919@163.com.
authors: Wen-Jing
authors: WJ
authors: Sun
authors: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
authors: Shuhao
authors: S
authors: Huo
authors: National Engineering Research Center of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, People's Republic of China. yangyan@saas.sh.cn.
authors: Yan
authors: Y
authors: Yang
mesh_terms: D000332:Aerobiosis; D018533:Biomass; D062610:Fungal Polysaccharides / Q000096:biosynthesis / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D048691:Grifola / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D008358:Mannose / Q000737:chemistry; D025282:Mycelium / Q000737:chemistry / Q000378:metabolism
chemical_list: D062610:Fungal Polysaccharides; D005947:Glucose; D008358:Mannose
keywords: Aeration rate; Agitation intensity; Exo-polymer; Grifola frondosa; Morphology; Polysaccharide compositions