title: Inhibitory function of NKT cells during early induction phase of nickel allergy.
abstract: Until now, metal allergies have been regarded as a Th1-type immune response.　However, because the contribution of a Th2-type immune response has been suggested by clinical findings, we previously examined the Th2-type immune response during the development of metal allergies using a GATA-3 transgenic (GATA-3 Tg) mouse model. As a result, a Th2-type immunization reaction was suggested to be involved in the early phase of metal allergies. Recently, the involvement of NKT cells in metal allergies has been suggested. We examined this possibility using the activation of NKT cells and an NKT cell-deficient mouse model to determine the contribution of NKT cells to nickel allergy in the present study. In NKT cell-deficient mice, ear swelling was remarkably increased, compared with that in control mice. Also, in mice that had been treated with α-galactosylceramide (α-GalCer) to activate NKT cells, the ear swelling response was remarkably inhibited, compared with that in untreated mice. These facts show that NKT cells are involved in the inhibition of nickel allergy-induced ear swelling responses.
authors: Division of Cellular Immunology, Kitasato University Graduate School of Medical Science, Kitasato, 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
authors: Hironori
authors: H
authors: Okuno
authors: Division of Cellular Immunology, Kitasato University Graduate School of Medical Science, Kitasato, 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
authors: Masashi
authors: M
authors: Satoh
authors: Division of Cellular Immunology, Kitasato University Graduate School of Medical Science, Kitasato, 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
authors: Emiko
authors: E
authors: Takeuchi
authors: Division of Cellular Immunology, Kitasato University Graduate School of Medical Science, Kitasato, 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
authors: Koji
authors: K
authors: Eshima
authors: Ehime Plant, Dainippon Sumitomo Pharma Co., Ltd., Sobiraki-cho, Niihama, Ehime, 792-0001, Japan.
authors: Masazumi
authors: M
authors: Terashima
authors: Department of Mechanical Engineering, Keio University, Hiyoshi 3-14-1, Kita, Kohoku-ku, Yokohama, Kanagawa, 223-8522, Japan.
authors: Jun
authors: J
authors: Komotori
authors: Department of Immunology, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan.
authors: Sonoko
authors: S
authors: Habu
authors: Division of Cellular Immunology, Kitasato University Graduate School of Medical Science, Kitasato, 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan; Division of Fundamental Medical Technology, Department of Immunology, Ehime Prefecture University of Health Sciences, Takooda 543, Tobe-cho, Iyo-gun, Ehime, 791-2101, Japan. Electronic address: hidetama@epu.ac.jp.
authors: Hidekazu
authors: H
authors: Tamauchi
authors: Division of Cellular Immunology, Kitasato University Graduate School of Medical Science, Kitasato, 1-15-1, Minami-ku, Sagamihara, Kanagawa, 252-0373, Japan.
authors: Kazuya
authors: K
authors: Iwabuchi
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D055610:Antigens, CD1d / Q000235:genetics; D016207:Cytokines / Q000097:blood; D003877:Dermatitis, Contact / Q000276:immunology*; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics; D005699:Galactosylceramides / Q000276:immunology; D006968:Hypersensitivity, Delayed; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D055611:Natural Killer T-Cells / Q000276:immunology*; D009532:Nickel / Q000276:immunology; D012867:Skin / Q000276:immunology*
chemical_list: D000485:Allergens; D055610:Antigens, CD1d; D016207:Cytokines; D050990:GATA3 Transcription Factor; D005699:Galactosylceramides; C494748:Gata3 protein, mouse; C493154:alpha-galactosylceramide; D009532:Nickel
keywords: CD1d; NKT cells; Nickel allergy; α-GalCer