title: Antibiotic concentration and antibiotic-resistant bacteria in two shallow urban lakes after stormwater event.
abstract: Stormwater runoff is generally characterized as non-point source pollution. In the present study, antibiotic concentration and antibiotic susceptibilities of cultivable heterotrophic bacteria were investigated in two small shallow urban lakes before and after strong storm event. Several antibiotics, lactose-fermenting bacteria and cultivable heterotrophic bacteria concentrations increased in surface water and/or surface sediment of two small urban lakes (Lake Xuanwu and Wulongtan) after strong storm event. In general, the frequencies of bacteria showing resistance to nine antibiotics increased after storm event. Based on the 16S rRNA genes of 50 randomly selected isolates from each water sample of two lakes, Aeromonas and Bacillus were dominant genera in samples from two lakes, while genera Proteus and Lysinibacillus were the third abundant genera in Lake Xuanwu and Wulongtu, respectively. Presences of nine antibiotic resistance genes (ARGs) in the 100 isolates were detected and most of these isolates harbored at least two ARGs with different functions. The detection frequency of ARGs in Gram-negative isolates was higher than that in Gram-positive isolates. The most prevalent integron in 100 isolates was int(II) (n = 28), followed by int(I) (n = 17) and int(III) (n = 17). Our results indicate that strong storm events potentially contribute to the transfer of ARGs and antibiotic-resistant bacteria from land-sewer system to the urban Lakes.
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China. shzhang@hhu.edu.cn.
authors: Songhe
authors: S
authors: Zhang
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
authors: Si
authors: S
authors: Pang
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China. PFWang2005@hhu.edu.cn.
authors: PeiFang
authors: P
authors: Wang
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
authors: Chao
authors: C
authors: Wang
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
authors: Nini
authors: N
authors: Han
authors: Institute of Pearl River Water Resources Protection, Pearl River Water Resources Commission of Ministry of Water Resource, Guangzhou, 510635, China.
authors: Bin
authors: B
authors: Liu
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
authors: Bing
authors: B
authors: Han
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
authors: Yi
authors: Y
authors: Li
authors: Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
authors: Kwaku
authors: K
authors: Anim-Larbi
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005285:Fermentation; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000235:genetics; D052836:Heterotrophic Processes; D032023:Integrons; D060106:Lakes / Q000382:microbiology*; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011891:Rain; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S
keywords: Antibiotic-resistant bacteria; Antibiotics; Integron; Storm; Urban lake