title: Purification of hematopoietic stem cells from bone marrow.
abstract: Lifelong production of blood cells is sustained by hematopoietic stem cells (HSC) which reside at a very low frequency within the bone marrow (BM). The use of flow cytometry has been critical in establishing methods to isolate and characterize HSCs and their progenitors. For more than 30 years, researchers have uncovered many novel markers that when used in combination significantly enhance the purification of HSCs from murine and human bone marrow. Here, we review the phenotypic markers and strategies used to purify HSCs.
authors: National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, People's Republic of China.
authors: Chen
authors: C
authors: Tian
authors: National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, People's Republic of China. yizhuozhang111@163.com.
authors: Yizhuo
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D051379:Mice
keywords: Flow cytometry; Hematopoietic stem cell; Markers; Purification