title: Effects of Aqueous Extract of Phyllostachyos Caulis in Taeniam on Longitudinal Bone Growth in Adolescent Rats.
abstract: This study examined whether treatment with Phyllostachyos Caulis in Taeniam aqueous extract improves longitudinal bone growth in adolescent male rats. Three-week-old male Sprague-Dawley rats were divided into three groups: a control group, a Phyllostachyos Caulis in Taeniam group (200 mg/kg, p.o.), and a recombinant human growth hormone group (20 µg/kg, s.c.). The total tibial length and the height of each growth plate zone were evaluated by radiography and histomorphometry. The total number of proliferative cells and 5-bromo-2'-deoxyuridine-positive cells were counted after 5-bromo-2'-deoxyuridine staining. Serum total osteocalcin levels were assayed using an enzyme-linked immunosorbent assay. The average total tibial length of the Phyllostachyos Caulis in Taeniam group was significantly longer than that of the control group. The heights of the proliferative and hypertrophic zones in the Phyllostachyos Caulis in Taeniam group were increased, and the ratio of 5-bromo-2'-deoxyuridine-positive to total cells in the proliferative zone was also increased. The serum osteocalcin, growth hormone, and insulin-like growth factor-1 levels were significantly increased in the Phyllostachyos Caulis in Taeniam group compared to the control group. Insulin-like growth factor-1 and insulin-like growth factor-1 receptor were highly expressed in the proliferative and hypertrophic zones in the Phyllostachyos Caulis in Taeniam group. The Phyllostachyos Caulis in Taeniam extract increased bone length, promoted cell proliferation, and activated the growth plate zones, which suggested that the extract could play an important role in longitudinal bone growth. Therefore, the Phyllostachyos Caulis in Taeniam extract might be a good alternative medicine to growth hormone therapy.
authors: Department of Anatomy, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
authors: Yoon Hee
authors: YH
authors: Chung
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
authors: Do Yeon
authors: DY
authors: Lee
authors: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
authors: Ho Sung
authors: HS
authors: Lee
authors: Department of Pathology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
authors: Soon Auck
authors: SA
authors: Hong
authors: Department of Pathology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
authors: Eon Sub
authors: ES
authors: Park
authors: Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chunchon, Republic of Korea.
authors: Yunsung
authors: Y
authors: Nam
authors: Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chunchon, Republic of Korea.
authors: Hyoung Chun
authors: HC
authors: Kim
authors: Department of Industrial Pharmacy, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
authors: Sang Joon
authors: SJ
authors: Lee
authors: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
authors: Uy Dong
authors: UD
authors: Sohn
authors: Korea Medicine Research Institute, Inc., Gyeonggi-do, Republic of Korea.
authors: Hohyun
authors: H
authors: Kim
authors: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
authors: Ji Hoon
authors: JH
authors: Jeong
mesh_terms: D000818:Animals; D031723:Bambusa / Q000737:chemistry*; D001835:Body Weight; D001846:Bone Development / Q000187:drug effects*; D001973:Bromodeoxyuridine; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D004435:Eating; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000187:drug effects / Q000254:growth & development
chemical_list: D010936:Plant Extracts; D001973:Bromodeoxyuridine