title: Foodomics study on the effects of extracellular production of hydrogen peroxide by rosemary polyphenols on the anti-proliferative activity of rosemary polyphenols against HT-29 cells.
abstract: A number of studies have demonstrated a strong association between the antioxidant properties of rosemary polyphenols and their chemoprotective activity. However, the prooxidant effects of rosemary polyphenols have been rarely reported. In this work, a foodomics study is performed to investigate the in vitro autooxidation of carnosic acid (CA), carnosol (CS) and a polyphenol-enriched rosemary extract (SC-RE) in cell culture conditions. The results revealed that rosemary polyphenols autooxidation in culture medium generated H2 O2 at different rates. Generated H2 O2 levels by SC-RE and CA, but not CS, were correlated with intracellular reactive oxygen species (ROS) generation in HT-29 cells, and were partially involved in their anti-proliferative effect in this cell line. These compounds also induced different effects on glutathione metabolism. Results also indicated that high extracellular H2 O2 concentrations, resulting of using high (45 μg/mL) SC-RE concentration in culture media, exerted some artifactual effects related with cell cycle, but they did not influence the expression of relevant molecular biomarkers of stress.
authors: Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Madrid, Spain.
authors: Alberto
authors: A
authors: Valdés
authors: Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Madrid, Spain.
authors: Virginia
authors: V
authors: García-Cañas
authors: Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Madrid, Spain.
authors: Engin
authors: E
authors: Koçak
authors: Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Madrid, Spain.
authors: Carolina
authors: C
authors: Simó
authors: Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Madrid, Spain.
authors: Alejandro
authors: A
authors: Cifuentes
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003470:Culture Media; D005504:Food Analysis; D019073:HT29 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D059808:Polyphenols / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D027542:Rosmarinus / Q000737:chemistry*
chemical_list: D003470:Culture Media; D059808:Polyphenols; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Antioxidant; Colon cancer; Glutathione; Prooxidant; Reactive oxygen species