title: Spherical silver nanoparticles in the detection of thermally denatured collagens.
abstract: We have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation. Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution of pure collagen was 14.7, 8.5, and 8.6 μg mL(-1) for porcine (PCOL), rat tail (RCOL), and type I human recombinant (HCOL) collagen, respectively.
authors: Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Rm H5229, Ottawa, Ontario, K1Y 4W7, Canada.|Laboratorio de Cinética y Fotoquímica, Departamento de Ciencias del Ambiente-Facultad de Química y Biología, Universidad de Santiago de Chile, Avenida Libertador Bernardo O´higgins 3363, 8370071, Santiago, Chile.
authors: Manuel
authors: M
authors: Ahumada
authors: Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Rm H5229, Ottawa, Ontario, K1Y 4W7, Canada.
authors: Sarah
authors: S
authors: McLaughlin
authors: Departamento de Química Orgánica-Facultad de Ciencias Químicas, INFIQC-CONICET and Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende s/n, X5000HUA, Ciudad Universitaria, 5000, Córdoba, Argentina. nataliap@fcq.unc.edu.ar.
authors: Natalia L
authors: NL
authors: Pacioni
authors: Bio-nanomaterials Chemistry and Engineering Laboratory, Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Rm H5229, Ottawa, Ontario, K1Y 4W7, Canada. ealarcon@ottawaheart.ca.|Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, K1Y 4W7, Canada. ealarcon@ottawaheart.ca.
authors: Emilio I
authors: EI
authors: Alarcon
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis / Q000737:chemistry*; D003124:Colorimetry / Q000191:economics / Q000379:methods; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D000066970:Protein Corona / Q000032:analysis / Q000737:chemistry; D011489:Protein Denaturation; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013552:Swine; D013696:Temperature
chemical_list: D000066970:Protein Corona; D011994:Recombinant Proteins; D012834:Silver; D003094:Collagen
keywords: Collagen; Nanoparticles/Nanotechnology; Surface plasmon band