title: Quantitative succinylome analysis in the liver of non-alcoholic fatty liver disease rat model.
abstract: BACKGROUND
  Non-alcoholic fatty liver disease (NAFLD) is a clinical frequent disease. However, its pathogenesis still needs further study, especially the mechanism at the molecular level. The recent identified novel protein post-translational modification, lysine succinylation was reported involved in diverse metabolism and cellular processes. In this study, we performed the quantitative succinylome analysis in the liver of NAFLD model to elucidate the regulatory role of lysine succinylation in NAFLD progression.
  
  
  METHODS
  Firstly, experimental model of NAFLD was induced by carbon tetrachloride injection and supplementary high-lipid and low-protein diet. Then series histochemical and biochemical variables were determined. For the quantitative succinylome analysis, tandem mass tags (TMT)-labeling, highly sensitive immune-affinity purification, liquid chromatography-tandem mass spectrometry techniques were applied. Bioinformatics analysis including gene ontology annotation based classification; Wolfpsort based subcellular prediction; function enrichment; protein-protein interaction network construction and conserved succinylation site motifs extraction were performed to decipher the differentially changed succinylated proteins and sites and p-value < 0.05 was selected as threshold.
  
  
  RESULTS
  Totally, 815 succinylation sites on 407 proteins were identified, of which 243 succinylation acetylation sites on 178 proteins showed changed succinylation level with the threshold fold change > 1.5. Theses differentially changed succinylated proteins were involved in diverse metabolism pathways and cellular processes including carbon metabolism, amino acid metabolism, fat acid metabolism, binding and catalyzing, anti-oxidation and xenobiotics metabolism. Besides, these differentially changed succinylated proteins were prominently localized to cytoplasm and mitochondria. Moreover, 8 conserved succinylation site motifs were extracted around the succinylation sites.
  
  
  CONCLUSIONS
  Protein succinylation was an extensive post-translation modification in rat. The changed succinylation level in diverse proteins may disturb multiple metabolism pathways and promote non-alcoholic fatty liver disease development. This study provided a basis for further characterization of the pathophysiological role of lysine succinylation in NAFLD progression, which laid a foundation for the innovation of novel NAFLD drugs and therapies.
authors: Department of liver disease, Hospital for Infectious Diseases of Pudong New Area, Shanghai, 201299 P. R. China ; Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203 P. R. China.
authors: Yang
authors: Y
authors: Cheng
authors: Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203 P. R. China.
authors: Tianlu
authors: T
authors: Hou
authors: Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203 P. R. China.
authors: Jian
authors: J
authors: Ping
authors: Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203 P. R. China.
authors: Gaofeng
authors: G
authors: Chen
authors: Department of liver disease, Hospital for Infectious Diseases of Pudong New Area, Shanghai, 201299 P. R. China ; Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203 P. R. China.
authors: Jianjie
authors: J
authors: Chen
keywords: Bioinformatics analysis; Lysine succinylation; Non-alcoholic fatty liver disease; Succinylome