title: Investigating the compatibility of the biocontrol agent Clonostachys rosea IK726 with prodigiosin-producing Serratia rubidaea S55 and phenazine-producing Pseudomonas chlororaphis ToZa7.
abstract: This study was carried out to assess the compatibility of the biocontrol fungus Clonostachys rosea IK726 with the phenazine-producing Pseudomonas chlororaphis ToZa7 or with the prodigiosin-producing Serratia rubidaea S55 against Fusarium oxysporum f. sp. radicis-lycopersici. The pathogen was inhibited by both strains in vitro, whereas C. rosea displayed high tolerance to S. rubidaea but not to P. chlororaphis. We hypothesized that this could be attributed to the ATP-binding cassette (ABC) proteins. The results of the reverse transcription quantitative PCR showed an induction of seven genes (abcB1, abcB20, abcB26, abcC12, abcC12, abcG8 and abcG25) from subfamilies B, C and G. In planta experiments showed a significant reduction in foot and root rot on tomato plants inoculated with C. rosea and P. chlororaphis. This study demonstrates the potential for combining different biocontrol agents and suggests an involvement of ABC transporters in secondary metabolite tolerance in C. rosea.
authors: Laboratory of Plant Pathology, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 269, 541 24, Thessaloniki, Greece. nathalie_2124@hotmail.com.
authors: Nathalie N
authors: http://orcid.org/0000-0003-3103-6252
authors: NN
authors: Kamou
authors: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, 75007, Uppsala, Sweden.
authors: Mukesh
authors: M
authors: Dubey
authors: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, 75007, Uppsala, Sweden.|Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Box 7080, 75007, Uppsala, Sweden.
authors: Georgios
authors: G
authors: Tzelepis
authors: Laboratory of Agronomy, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 269, 541 24, Thessaloniki, Greece.
authors: Georgios
authors: G
authors: Menexes
authors: Laboratory of Pesticide Science, Department of Agriculture, Aristotle University of Thessaloniki, P.O. Box 1678, 540 06, Thessaloniki, Greece.
authors: Emmanouil N
authors: EN
authors: Papadakis
authors: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, 75007, Uppsala, Sweden.
authors: Magnus
authors: M
authors: Karlsson
authors: Laboratory of Plant Pathology, School of Agriculture, Aristotle University of Thessaloniki, P.O. Box 269, 541 24, Thessaloniki, Greece.
authors: Anastasia L
authors: AL
authors: Lagopodi
authors: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, 75007, Uppsala, Sweden.
authors: Dan Funck
authors: DF
authors: Jensen
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D005670:Fusarium / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D006999:Hypocreales / Q000235:genetics / Q000502:physiology*; D018551:Solanum lycopersicum / Q000382:microbiology; D056265:Microbial Interactions / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D018517:Plant Roots / Q000382:microbiology; D011549:Pseudomonas / Q000235:genetics / Q000502:physiology*; D012705:Serratia / Q000502:physiology*
chemical_list: D018528:ATP-Binding Cassette Transporters
keywords: ABC transporter; Biocontrol; Clonostachys rosea; Prodigiosin; Pseudomonas chlororaphis; Serratia rubidaea