title: Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.
abstract: A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany. abutkev@gwdg.de.
authors: Alexey N
authors: AN
authors: Butkevich
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Gyuzel Yu
authors: http://orcid.org/0000-0003-4376-6981
authors: GY
authors: Mitronova
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Sven C
authors: http://orcid.org/0000-0003-1830-5489
authors: SC
authors: Sidenstein
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Jessica L
authors: JL
authors: Klocke
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Dirk
authors: D
authors: Kamin
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Dirk N H
authors: DN
authors: Meineke
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Elisa
authors: http://orcid.org/0000-0003-0781-187X
authors: E
authors: D'Este
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Philip-Tobias
authors: PT
authors: Kraemer
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
authors: Johann G
authors: http://orcid.org/0000-0001-8559-3973
authors: JG
authors: Danzl
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany. vbelov@gwdg.de.
authors: Vladimir N
authors: http://orcid.org/0000-0002-7741-4653
authors: VN
authors: Belov
authors: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany. shell@gwdg.de.
authors: Stefan W
authors: SW
authors: Hell
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy / Q000379:methods*; D011754:Pyronine / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D011754:Pyronine
keywords: carbopyronines; fluorescence; living cells; optical microscopy; rhodamines