title: Sensory and optogenetically driven single-vessel fMRI.
abstract: Magnetic resonance imaging (MRI) sensitivity approaches vessel specificity. We developed a single-vessel functional MRI (fMRI) method to image the contribution of vascular components to blood oxygenation level-dependent (BOLD) and cerebral blood volume (CBV) fMRI signal. We mapped individual vessels penetrating the rat somatosensory cortex with 100-ms temporal resolution by MRI with sensory or optogenetic stimulation. The BOLD signal originated primarily from venules, and the CBV signal from arterioles. The single-vessel fMRI method and its combination with optogenetics provide a platform for mapping the hemodynamic signal through the neurovascular network with specificity at the level of individual arterioles and venules.
authors: High Field Magnetic Resonance Department, Max Planck Institute for Biological Cybernetics, Tuebingen, Germany.
authors: Xin
authors: X
authors: Yu
authors: High Field Magnetic Resonance Department, Max Planck Institute for Biological Cybernetics, Tuebingen, Germany.
authors: Yi
authors: Y
authors: He
authors: High Field Magnetic Resonance Department, Max Planck Institute for Biological Cybernetics, Tuebingen, Germany.
authors: Maosen
authors: M
authors: Wang
authors: Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
authors: Hellmut
authors: H
authors: Merkle
authors: Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
authors: Stephen J
authors: SJ
authors: Dodd
authors: Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
authors: Afonso C
authors: http://orcid.org/0000-0002-6105-7567
authors: AC
authors: Silva
authors: Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA.
authors: Alan P
authors: AP
authors: Koretsky
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000502:physiology*; D001931:Brain Mapping / Q000379:methods*; D002560:Cerebrovascular Circulation; D006439:Hemodynamics; D008279:Magnetic Resonance Imaging / Q000379:methods*; D062308:Optogenetics / Q000379:methods*; D010100:Oxygen / Q000097:blood*; D051381:Rats; D013003:Somatosensory Cortex / Q000098:blood supply / Q000166:cytology / Q000502:physiology*
chemical_list: D010100:Oxygen