title: Massilia humi sp. nov. isolated from soil in Incheon, South Korea.
abstract: A Gram-negative, strictly aerobic, non-motile, rod-shaped bacterium, designated THG-S6.8(T), was isolated from soil in Incheon, South Korea. Based on 16S rRNA gene sequence, strain THG-S6.8(T) was moderately related to Massilia plicata 76(T) (97.3 %), Massilia dura 16(T) (97.2 %) and Massilia albidiflava 45(T) (96.9 %). Chemotaxonomic data revealed that strain THG-S6.8(T) possesses ubiquinone-8 as the predominant respiratory quinone, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C16:0 as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA-DNA relatedness between strain THG-S6.8(T) and Massilia plicata KCTC 12344(T) and Massilia dura KACC 12342(T) was 38.7 and 40.5 %, respectively. The DNA G+C content was 66.8 %. These data, together with phenotypic characterization, suggest the isolate represents a novel species, for which the name Massilia humi sp. nov. is proposed, with THG-S6.8(T) as the type strain (=KCTC 42737(T) = CCTCC AB 2015296(T)).
authors: Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi, 446-701, Republic of Korea.
authors: Juan
authors: J
authors: Du
authors: Department of Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, # 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. acuyin@khu.ac.kr.
authors: Chang Shik
authors: CS
authors: Yin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D042802:Oxalobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8
keywords: 16S rRNA; Massilia humi; Ubiquinone-8