title: Structure and gene cluster of the O-antigen of Escherichia coli O137.
abstract: The O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Escherichia coli O137 and studied by sugar analysis and NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit was established: Formula: see text] Both structure and gene cluster of the E. coli O137 polysaccharide are related to those of the E. coli K40 polysaccharide (Amor et al., 1999), which lacks the side-chain glucosylation but contains serine that is amide-linked to GlcA. Functions of genes in the O137-antigen gene cluster were assigned by a comparison with those in K40 and sequences in the available databases. Particularly, predicted glycosyltransferases encoded in the gene cluster were assigned to the formation of three glycosidic linkages in the O-polysaccharide repeating unit.
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
authors: Andrei V
authors: AV
authors: Perepelov
authors: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China.
authors: Xi
authors: X
authors: Guo
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Sof'ya N
authors: SN
authors: Senchenkova
authors: The Third Central Hospital of Tianjin, Hedong District, Tianjin 300170, China.
authors: Yayue
authors: Y
authors: Li
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Alexander S
authors: AS
authors: Shashkov
authors: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China.
authors: Bin
authors: B
authors: Liu
authors: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*
chemical_list: D019081:O Antigens
keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen; O–antigen gene cluster