title: Genetic regulation and manipulation for natural product discovery.
abstract: Natural products are an important source of modern medical development, e.g., antibiotics, anticancers, immune modulators, etc. and will continue to be a powerful driving force for the discovery of novel potential drugs. In the heterologous hosts, natural products are biosynthesized using dedicated metabolic networks. By gene engineering, pathway reconstructing, and enzyme engineering, metabolic networks can be modified to synthesize novel compounds containing enhanced structural feature or produce a large quantity of known valuable bioactive compounds. The review introduces some important technical platforms and relevant examples of genetic regulation and manipulation to improve natural product titers or drive novel secondary metabolite discoveries.
authors: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China. cjw983617@gmail.com.
authors: Jianwei
authors: J
authors: Chen
authors: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China. biop218_wuqihao@sina.com.
authors: Qihao
authors: Q
authors: Wu
authors: Marine Chemistry Department, Faculty of Marine Science, King Abdulaziz University, P.O. Box 80207, Jeddah, 21589, Kingdom of Saudi Arabia. hawasusama@yahoo.com.
authors: Usama W
authors: UW
authors: Hawas
authors: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 310014, China. hongw@zjut.edu.cn.|Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310014, China. hongw@zjut.edu.cn.
authors: Hong
authors: H
authors: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D001688:Biological Products / Q000302:isolation & purification / Q000378:metabolism*; D053898:Biosynthetic Pathways; D055808:Drug Discovery; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D006020:Glycopeptides / Q000096:biosynthesis / Q000302:isolation & purification; D006801:Humans; D007155:Immunologic Factors / Q000096:biosynthesis / Q000302:isolation & purification; D009285:Naphthoquinones / Q000302:isolation & purification / Q000378:metabolism; D064210:Secondary Metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D001688:Biological Products; D006020:Glycopeptides; D007155:Immunologic Factors; D009285:Naphthoquinones
keywords: Discovery; Natural product; Regulation and manipulation