title: Novel immunoassay and rapid immunoaffinity chromatography method for the detection and selective extraction of naringin in Citrus aurantium.
abstract: In this work, a novel monoclonal antibody specific for naringin was prepared and characterized. Subsequently, an indirect competitive enzyme-linked immunosorbent assay for naringin was developed, with an effective range from 4.8 to 156 ng/mL naringin. Next, an immunoaffinity column was obtained by coupling anti-naringin monoclonal antibodies to CNBr-activated Sepharose 4B and a rapid immunoaffinity chromatography assay for naringin was developed. The immunoaffinity column was used to separate naringin from Citrus aurantium. The results showed that 1 g of the dry Sepharose 4B can couple 10 mg of immunoglobulin G. And the immunoaffinity column can efficiently and specifically capture approximately 250 μg of naringin without cross reacting with its structurally similar compounds. Moreover, our results indicate that the application of immunoaffinity chromatography can simplify the pretreatment and the isolation process greatly compared to conventional methods, providing a potential method for extracting the target component from structurally similar compounds in natural products.
authors: Centre of Scientific Experiment, Beijing University of Chinese Medicine, Beijing, China.
authors: Huihua
authors: H
authors: Qu
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors: Yue
authors: Y
authors: Zhang
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors: Baoping
authors: B
authors: Qu
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors: Jinjun
authors: J
authors: Cheng
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Shuchen
authors: S
authors: Liu
authors: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors: Shenglan
authors: S
authors: Feng
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Qingguo
authors: Q
authors: Wang
authors: School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, China.
authors: Yan
authors: Y
authors: Zhao
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification*; D007118:Immunoassay / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D044950:Flavanones; C005274:naringin
keywords: Enzyme-linked immunosorbent assay; Immunoaffinity chromatography; Monoclonal antibodies; Naringin