title: Molecular and antibiotic susceptibility characterization of Aerococcus viridans isolated from porcine urinary infection.
abstract: Aerococcus viridans has been reported as a human and animal pathogen causing urinary tract infection, arthritis, pneumonia, meningitis and endocarditis. Routinely, A. viridans is not surveyed in clinical diagnosis laboratories and commonly is misidentified as other bacteria. There is no concrete data on the prevalence and impact of the pathogen to both human and animal health. In the present study, we report the isolation and molecular and antibiotic susceptibility characterization of A. viridans strains from porcine urinary infections. A total of 22 isolates were identified as A. viridans by MALDI-TOF MS and confirmed by 16S rRNA gene sequencing. Isolates were genotyped by single enzyme amplified fragments length polymorphism (SE-AFLP) that resulted in 19 clusters of which 81.2% were composed by single isolates. The high genetic heterogeneity corroborates previous studies and appears to be a particularity of A. viridans. The minimal inhibitory concentration (MIC) values also presented variability especially for ceftiofur, fluoroquinolones and aminoglycosides. The high MICs of aminoglycosides, tetracyclines and macrolides seen among the A. viridans corroborate previous reports and the widespread veterinary usage of these antibiotics demand attention for the implication of A. viridans infection to both human and animal health.
authors: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil. Electronic address: luzanolli@gmail.com.
authors: Luisa Z
authors: LZ
authors: Moreno
authors: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil. Electronic address: carlos.cabrera@usp.br.
authors: Carlos E C
authors: CE
authors: Matajira
authors: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil. Electronic address: vascotulio@usp.br.
authors: Vasco T M
authors: VT
authors: Gomes
authors: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil. Electronic address: anapaula_silva2006@yahoo.com.br.
authors: Ana Paula S
authors: AP
authors: Silva
authors: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil. Electronic address: renan612@gmail.com.
authors: Renan E
authors: RE
authors: Mesquita
authors: Companhia Ambiental do Estado de São Paulo (CETESB). Av Prof Frederico Hermann Jr, 345; 05459-900; Pinheiros. São Paulo/SP, Brazil. Electronic address: anacamps@gmail.com.
authors: Ana Paula G
authors: AP
authors: Christ
authors: Companhia Ambiental do Estado de São Paulo (CETESB). Av Prof Frederico Hermann Jr, 345; 05459-900; Pinheiros. São Paulo/SP, Brazil. Electronic address: minesato@osite.com.br.
authors: Maria Inês Z
authors: MI
authors: Sato
authors: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo. Av Prof Dr Orlando Marques de Paiva, 87; Cidade Universitária. São Paulo/SP, Brazil. Electronic address: morenoam@usp.br.
authors: Andrea M
authors: AM
authors: Moreno
mesh_terms: D056570:Aerococcus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005838:Genotype; D008826:Microbial Sensitivity Tests; D012336:RNA, Ribosomal, 16S; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S
keywords: Aerococcus viridans; MIC; porcine; sequencing