title: Characterization of lesion formation in marmosets following inhalational challenge with different strains of Burkholderia pseudomallei.
abstract: The marmoset model of melioidosis was used to explore whether there was any difference in the disease presentation and/or the lesion formation following inhalational challenge with one of four strains of Burkholderia pseudomallei (K96243, 1026b, HBPUB10303a and HBPUB10134a). Marmosets were challenged with a range of bacterial doses and bacterial load, histological and physiological features were determined temporally following lethal disease. Melioidosis presented as an acute, febrile disease with bacteraemia, bacterial dissemination, necrotizing hepatitis, splenitis and pneumonia which was independent of the challenge strain. Generally, there were no major differences in the manifestation of melioidosis following challenge by the different strains of B. pseudomallei; however, there were some differences in the time to death and the severity of the pathological features. The pathological features observed in the liver and spleen of animals challenged with B. pseudomallei strain 1026b were statistically less severe (P < 0.05) and less frequent. However, more severe foci of disease were evident in the lungs of animals challenged with strain 1026b. In all cases, the lesions developed from small areas of bacteria-infected macrophages surrounded by non-infected neutrophils into large lesions with both immune cell types infected. The marmoset model was a useful tool enabling the distinction of subtle difference in the pathological response to B. pseudomallei.
authors: Microbiology Group, CBR Division, Dstl, Salisbury, Wiltshire, UK.
authors: Michelle
authors: M
authors: Nelson
authors: Animal Plant and Health Agency, Addlestone, Surrey, UK.
authors: Alejandro
authors: A
authors: Nunez
authors: Microbiology Group, CBR Division, Dstl, Salisbury, Wiltshire, UK.
authors: Sarah A
authors: SA
authors: Ngugi
authors: Microbiology Group, CBR Division, Dstl, Salisbury, Wiltshire, UK.
authors: Adam
authors: A
authors: Sinclair
authors: Microbiology Group, CBR Division, Dstl, Salisbury, Wiltshire, UK.
authors: Timothy P
authors: TP
authors: Atkins
mesh_terms: D000336:Aerosols; D000818:Animals; D058491:Bacterial Load; D016957:Burkholderia pseudomallei / Q000145:classification / Q000472:pathogenicity*; D002144:Callithrix; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D054884:Host-Pathogen Interactions; D019570:Inhalation Exposure; D008099:Liver / Q000382:microbiology / Q000473:pathology*; D008168:Lung / Q000382:microbiology / Q000473:pathology*; D008264:Macrophages / Q000382:microbiology / Q000473:pathology; D008297:Male; D008554:Melioidosis / Q000382:microbiology / Q000473:pathology*; D009504:Neutrophils / Q000473:pathology; D012720:Severity of Illness Index; D013154:Spleen / Q000382:microbiology / Q000473:pathology*; D013997:Time Factors
chemical_list: D000336:Aerosols
keywords: NHP; animal model; melioidosis