title: Aerobic degradation of BDE-209 by Enterococcus casseliflavus: Isolation, identification and cell changes during degradation process.
abstract: Decabromodiphenyl ether (BDE-209) is one of the most commonly used brominated flame retardants that have contaminated the environment worldwide. Microbial bioremediation has been considered as an effective technique to remove these sorts of persistent organic pollutants. Enterococcus casseliflavus, a gram-positive bacterium capable of aerobically transforming BDE-209, was isolated by our team from sediments in Guiyu, an e-waste dismantling area in Guangdong Province, China. To promote microbial bioremediation of BDE-209 and elucidate the mechanism behind its aerobic degradation, the effects of BDE-209 on the cell changes of E. casseliflavus were examined in this study. The experimental results demonstrated that the high cell surface hydrophobicity (CSH) of E. casseliflavus made the bacteria absorb hydrophobic BDE-209 more easily. E. casseliflavus responded to BDE-209 stress, resulting in an increase in cell membrane permeability and accumulation of BDE-209 inside the cell. The differential expression of intracellular protein was analyzed through two-dimensional gel electrophoresis (2-DE). More than 50 differentially expressed protein spots were reproducibly detected, including 25 up, and 25 down regulated after a 4 days exposure. Moreover, the apoptotic-like cell changes were observed during E. casseliflavus mediated degradation of BDE-209 by means of flow cytometry.
authors: Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
authors: Shaoyu
authors: S
authors: Tang
authors: Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China. Electronic address: huayin@scut.edu.cn.
authors: Hua
authors: H
authors: Yin
authors: College of Natural Resource and Environment, South China Agriculture University, Guangzhou 510642, Guangdong, China.
authors: Shuona
authors: S
authors: Chen
authors: Department of Chemistry, Jinan University, Guangzhou 510632, Guangdong, China.
authors: Hui
authors: H
authors: Peng
authors: College of Natural Resource and Environment, South China Agriculture University, Guangzhou 510642, Guangdong, China.
authors: Jingjing
authors: J
authors: Chang
authors: Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
authors: Zehua
authors: Z
authors: Liu
authors: Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
authors: Zhi
authors: Z
authors: Dang
mesh_terms: D000332:Aerobiosis; D017209:Apoptosis; D001673:Biodegradation, Environmental; D002463:Cell Membrane Permeability; D002681:China; D059029:Electronic Waste; D016983:Enterococcus / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D005411:Flame Retardants / Q000378:metabolism* / Q000633:toxicity; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity
chemical_list: D004785:Environmental Pollutants; D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; C010902:decabromobiphenyl ether
keywords: Apoptotic-like cell changes; Cellular characteristics; Decabromodiphenyl ether; Differentially expressed protein; Enterococcus casseliflavus