title: Zebrafish Embryonic Lipidomic Analysis Reveals that the Yolk Cell Is Metabolically Active in Processing Lipid.
abstract: The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis.
authors: Metabolic Genetic Diseases Laboratory, Metabolic Research Unit, Deakin University School of Medicine, 75 Pigdons Road, Geelong, VIC 3216, Australia.
authors: Daniel
authors: D
authors: Fraher
authors: Metabolic Genetic Diseases Laboratory, Metabolic Research Unit, Deakin University School of Medicine, 75 Pigdons Road, Geelong, VIC 3216, Australia.
authors: Andrew
authors: A
authors: Sanigorski
authors: Baker IDI Heart and Diabetes Institute, 75 Commercial Road, Melbourne, VIC 3004, Australia.
authors: Natalie A
authors: NA
authors: Mellett
authors: Baker IDI Heart and Diabetes Institute, 75 Commercial Road, Melbourne, VIC 3004, Australia.
authors: Peter J
authors: PJ
authors: Meikle
authors: Metabolic Genetic Diseases Laboratory, Metabolic Research Unit, Deakin University School of Medicine, 75 Pigdons Road, Geelong, VIC 3216, Australia. Electronic address: andrew.sinclair@deakin.edu.au.
authors: Andrew J
authors: AJ
authors: Sinclair
authors: Metabolic Genetic Diseases Laboratory, Metabolic Research Unit, Deakin University School of Medicine, 75 Pigdons Road, Geelong, VIC 3216, Australia. Electronic address: y.gibert@deakin.edu.au.
authors: Yann
authors: Y
authors: Gibert
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000235:genetics / Q000378:metabolism; D001896:Boron Compounds / Q000008:administration & dosage; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D002853:Chromatography, Liquid; D047108:Embryonic Development / Q000235:genetics; D005456:Fluorescent Dyes / Q000008:administration & dosage; D018507:Gene Expression Regulation, Developmental; D050356:Lipid Metabolism / Q000235:genetics*; D013058:Mass Spectrometry; D055442:Metabolome; D058977:Molecular Sequence Annotation; D010713:Phosphatidylcholines / Q000378:metabolism*; D010740:Phospholipases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014280:Triglycerides / Q000378:metabolism*; D015017:Yolk Sac / Q000737:chemistry / Q000196:embryology / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001053:Apolipoproteins; D001896:Boron Compounds; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D010713:Phosphatidylcholines; D014280:Triglycerides; D002784:Cholesterol; D010740:Phospholipases
keywords: BODIPY; PPARγ; TLC; embryo body; embryogenesis; lipidomics; lipids; yolk sac; zebrafish