title: Tumor-targeting Salmonella typhimurium A1-R in combination with doxorubicin eradicate soft tissue sarcoma in a patient-derived orthotopic xenograft (PDOX) model.
abstract: A patient with high grade undifferentiated pleomorphic soft-tissue sarcoma from a striated muscle was grown orthotopically in the right biceps femoris muscle of mice to establish a patient-derived orthotopic xenograft (PDOX) model. Twenty PDOX mice were divided into 4 groups: G1, control without treatment; G2, Salmonella typhimurium (S. typhimurium)A1-R administered by intratumoral (i.t.) injection once a week for 4 weeks; G3, doxorubicin (DOX) administered by intraperitoneal (i.p.) injection once a week for 4 weeks; G4, S. typhimurium A1-R (i.t.) administered once a week for 2 weeks followed by i.p. doxorubicin once a week for 2 weeks. On day 25 from the initiation of treatment, tumor volume in G2, G3, and G4 was significantly lower than G1. Mice found without gross tumor included one mouse (20%) in G2; one mouse (20%) in G3; and 3 mice (60%) in G4. Body weight loss did not significantly differ between the 3 treated groups or from the untreated control. Histological examination revealed eradication of tumor only in G4 where mice were treated with S. typhimurium A1-R followed by DOX. Our present study indicates future clinical potential of combining S. typhimurium A1-R with chemotherapy such as DOX for soft tissue sarcoma patients.
authors: AntiCancer, Inc., San Diego, California, USA.|Department of Surgery, University of California, San Diego, California, USA.|Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors: Takashi
authors: T
authors: Murakami
authors: Department of Surgery, University of California, San Diego, California, USA.
authors: Jonathan
authors: J
authors: DeLong
authors: Division of Surgical Oncology, University of California, Los Angeles, California, USA.
authors: Fritz C
authors: FC
authors: Eilber
authors: AntiCancer, Inc., San Diego, California, USA.
authors: Ming
authors: M
authors: Zhao
authors: AntiCancer, Inc., San Diego, California, USA.
authors: Yong
authors: Y
authors: Zhang
authors: AntiCancer, Inc., San Diego, California, USA.
authors: Nan
authors: N
authors: Zhang
authors: Division of Hematology-Oncology, University of California, Los Angeles, California, USA.
authors: Arun
authors: A
authors: Singh
authors: Division of Surgical Oncology, University of California, Los Angeles, California, USA.
authors: Tara
authors: T
authors: Russell
authors: AntiCancer, Inc., San Diego, California, USA.
authors: Samantha
authors: S
authors: Deng
authors: AntiCancer, Inc., San Diego, California, USA.
authors: Jose
authors: J
authors: Reynoso
authors: AntiCancer, Inc., San Diego, California, USA.
authors: Cuong
authors: C
authors: Quan
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors: Yukihiko
authors: Y
authors: Hiroshima
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors: Ryusei
authors: R
authors: Matsuyama
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors: Takashi
authors: T
authors: Chishima
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors: Kuniya
authors: K
authors: Tanaka
authors: Department of Surgery, University of California, San Diego, California, USA.
authors: Michael
authors: M
authors: Bouvet
authors: Sarcoma Oncology Center, Santa Monica, California, USA.
authors: Sant
authors: S
authors: Chawla
authors: Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors: Itaru
authors: I
authors: Endo
authors: AntiCancer, Inc., San Diego, California, USA.|Department of Surgery, University of California, San Diego, California, USA.
authors: Robert M
authors: RM
authors: Hoffman
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D012480:Salmonella Infections; D012486:Salmonella typhimurium; D012509:Sarcoma / Q000473:pathology / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D004317:Doxorubicin
keywords: Salmonella typhimurium A1-R; nude mice; patient-derived orthotopic xenograft; soft tissue sarcoma; tumor-targeting