title: Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea.
abstract: Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage titers is noted at temperature 30-50 °C. A latent period of approximately 96 mins is indicated by the one-step growth curve. And, the burst size is about three virions per cell. Furthermore, genomic analysis of H188 reveals a genome size of 50364 bp with 43.63 % G+C content, and 76 putative open reading frames. There is no obvious similarity between H188 and other known phages by genomic comparison. Moreover, the H188 genome includes modules for phage structure, phage packaging, DNA replication and regulation, and some additional functions.
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
authors: Yan
authors: Y
authors: Li
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. mingwang@ouc.edu.cn.
authors: Min
authors: M
authors: Wang
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
authors: Qian
authors: Q
authors: Liu
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
authors: Xue
authors: X
authors: Song
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
authors: Duobing
authors: D
authors: Wang
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
authors: Yu
authors: Y
authors: Ma
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
authors: Hongbing
authors: H
authors: Shao
authors: College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. yongjiang@ouc.edu.cn.
authors: Yong
authors: Y
authors: Jiang
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D001482:Base Composition; D016679:Genome, Viral; D023281:Genomics; D017900:Myoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D009792:Oceans and Seas; D016366:Open Reading Frames; D010802:Phylogeny; D012623:Seawater / Q000821:virology*; D014733:Vibrio / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins