title: Insulin-Like Growth Factor II mRNA-Binding Protein 3 (IMP3) as a Useful Immunohistochemical Marker for the Diagnosis of Adenocarcinoma of Small Intestine.
abstract: The biological characteristics and roles of insulin-like growth factor II mRNA-binding protein 3 protein (IMP3) expression in small-intestinal adenocarcinoma were investigated. The value of IMP3 immunostaining in the diagnosis of small-intestinal epithelial lesions was also evaluated. Immunohistochemical expression of IMP3 in normal small-intestinal mucosa adjacent to adenoma and adenocarcinoma lesions, and inflamed duodenal and ileal mucosa was analyzed. Samples assessed were: duodenal ulcer (n=6), Crohn's disease (n=5), low-grade small-intestinal adenoma (n=10), high-grade small-intestinal adenoma (n=13), small-intestinal adenocarcinoma (n=23), lymph node metastases (LNM; n=7), and preoperative biopsies of small-intestinal adenocarcinoma (n=6). Immunohistochemical expression of Ki-67 and p53 was also analyzed in adenoma and adenocarcinoma samples. IMP3 was not expressed in normal epithelium, but weakly expressed in reparative epithelium. Meanwhile, increased IMP3 expression was associated with a higher degree of dysplasia in adenomas, higher T classification, LNM, Ki-67 positivity, histological differentiation, and lower 5-year disease-free survival, but not p53 expression in adenocarcinoma. IMP3 expression appears to be a late event in the small-intestinal carcinogenesis. Assessing the IMP3 staining pattern can be useful in the diagnosis of small-intestinal epithelial lesions when used in conjunction with other histological criteria.
authors: Department of Gastroenterology, Shinshu University School of Medicine , 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
authors: Seiichi
authors: S
authors: Daikuhara
authors: Department of Laboratory Medicine, Shinshu University School of Medicine , 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
authors: Takeshi
authors: T
authors: Uehara
authors: Department of Clinical Laboratory, Aizawa Hospital , 2-5-1 Honjou, Matsumoto, Nagano 390-8510, Japan.
authors: Kayoko
authors: K
authors: Higuchi
authors: Department of Laboratory Medicine, Nagano Municipal Hospital , 1333-1 Tomitake.
authors: Noriko
authors: N
authors: Hosaka
authors: Department of Pathology, Nagano Municipal Hospital , 1333-1 Tomitake, Nagano 381-8551, Japan.
authors: Mai
authors: M
authors: Iwaya
authors: Department of Gastroenterology, Shinshu University School of Medicine , 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
authors: Yasuhiro
authors: Y
authors: Maruyama
authors: Department of Laboratory Medicine, Shinshu University Hospital , Matsumoto, Nagano 390-8621, Japan.
authors: Kazuyuki
authors: K
authors: Matsuda
authors: Endoscopic Examination Center, Shinshu University Hospital , Matsumoto, Nagano 390-8621, Japan.
authors: Norikazu
authors: N
authors: Arakura
authors: Department of Gastroenterology, Shinshu University School of Medicine , 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
authors: Eiji
authors: E
authors: Tanaka
authors: Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine , 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
authors: Hiroyoshi
authors: H
authors: Ota
keywords: IMP3; adenocarcinoma; immunohistochemistry; p53; small intestine