title: Characterization of the aodA, dnmA, mnSOD and pimA genes in Aspergillus nidulans.
abstract: Mitochondria play key roles in cellular energy generation and lifespan of most eukaryotes. To understand the functions of four nuclear-encoded genes predicted to be related to the maintenance of mitochondrial morphology and function in Aspergillus nidulans, systematic characterization was carried out. The deletion and overexpression mutants of aodA, dnmA, mnSOD and pimA encoding alternative oxidase, dynamin related protein, manganese superoxide dismutase and Lon protease, respectively, were generated and examined for their growth, stress tolerances, respiration, autolysis, cell death, sterigmatocystin production, hyphal morphology and size, and mitochondrial superoxide production as well as development. Overall, genetic manipulation of these genes had less effect on cellular physiology and ageing in A. nidulans than that of their homologs in another fungus Podospora anserina with a well-characterized senescence. The observed interspecial phenotypic differences can be explained by the dissimilar intrinsic stabilities of the mitochondrial genomes in A. nidulans and P. anserina. Furthermore, the marginally altered phenotypes observed in A. nidulans mutants indicate the presence of effective compensatory mechanisms for the complex networks of mitochondrial defense and quality control. Importantly, these findings can be useful for developing novel platforms for heterologous protein production, or on new biocontrol and bioremediation technologies based on Aspergillus species.
authors: Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors: Éva
authors: É
authors: Leiter
authors: Departments of Bacteriology and Genetics, The University of Wisconsin-Madison, Wisconsin, USA.
authors: Hee-Soo
authors: HS
authors: Park
authors: Departments of Bacteriology and Genetics, The University of Wisconsin-Madison, Wisconsin, USA.
authors: Nak-Jung
authors: NJ
authors: Kwon
authors: Departments of Bacteriology and Genetics, The University of Wisconsin-Madison, Wisconsin, USA.|Department of Pharmaceutical Engineering, Woosuk University, Wanju, Republic of Korea.
authors: Kap-Hoon
authors: KH
authors: Han
authors: Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors: Tamás
authors: T
authors: Emri
authors: Department of Botany, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors: Viktor
authors: V
authors: Oláh
authors: Department of Botany, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors: Ilona
authors: I
authors: Mészáros
authors: Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors: Beatrix
authors: B
authors: Dienes
authors: Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors: János
authors: J
authors: Vincze
authors: Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors: László
authors: L
authors: Csernoch
authors: Departments of Bacteriology and Genetics, The University of Wisconsin-Madison, Wisconsin, USA.
authors: Jae-Hyuk
authors: JH
authors: Yu
authors: Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors: István
authors: I
authors: Pócsi
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001673:Biodegradation, Environmental; D034281:Dynamins / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D048468:Podospora / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D049070:Protease La / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D000099188:Alternative Oxidase
chemical_list: D034281:Dynamins; D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D049070:Protease La; D013482:Superoxide Dismutase; D000099188:Alternative Oxidase