title: A review of induced pluripotent stem cell, direct conversion by trans-differentiation, direct reprogramming and oligodendrocyte differentiation.
abstract: Rapid progress in the field of stem cell therapy and cellular reprogramming provides convincing evidence of its feasibility in treating a wide range of pathologies through autologous cell replacement therapy. This review article describes in detail on three widely used approaches of somatic cell reprogramming: induced pluripotent stem cells, direct conversion and direct reprogramming, in the context of demyelination in the CNS. The potential limitations of each reprogramming technique are reviewed along with their distinct molecular approach to reprogramming. This is followed by an analysis on the scopes and challenges of its translational applications in deriving oligodendrocyte progenitor cells and oligodendrocytes for cell replacement treatment of demyelinating conditions in the CNS.
authors: Departments of Orthopaedic Surgery, Biomedical Engineering & Medicine, Division of Neurology, Singapore Institute of Neurotechnology, National University of Singapore, Singapore.
authors: Ankshita
authors: A
authors: Prasad
authors: Orthopaedic Surgery, Medicine, Division of Neurology, National University of Singapore, Singapore.
authors: Janani
authors: J
authors: Manivannan
authors: Departments of Orthopaedic Surgery, Biomedical Engineering & Medicine, Division of Neurology, Singapore Institute of Neurotechnology, National University of Singapore, Singapore.
authors: Daniel T B
authors: DT
authors: Loong
authors: Departments of Orthopaedic Surgery, Biomedical Engineering & Medicine, Division of Neurology, Singapore Institute of Neurotechnology, National University of Singapore, Singapore.
authors: Soo M
authors: SM
authors: Chua
authors: Departments of Biomedical Engineering & Neurology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
authors: Payam M
authors: PM
authors: Gharibani
authors: Departments of Orthopaedic Surgery, Biomedical Engineering & Medicine, Division of Neurology, Singapore Institute of Neurotechnology, National University of Singapore, Singapore.|Orthopaedic Surgery, Medicine, Division of Neurology, National University of Singapore, Singapore.|Departments of Biomedical Engineering & Neurology, Johns Hopkins School of Medicine, Baltimore, MD, USA.|Medicine, Division of Neurology, National University of Singapore, Singapore.|Biomedical Engineering & Medicine, Division of Neurology, National University of Singapore, Singapore.|Neurology, Johns Hopkins School of Medicine, Baltimore, MD, USA.|Singapore Institute of Neurotechnology, Centre for Life Sciences, National University of Singapore, 28 Medical Drive, #05-COR, Singapore 117456.
authors: Angelo H
authors: AH
authors: All
mesh_terms: D000818:Animals; D064592:Autografts; D054338:Cell Transdifferentiation; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D003711:Demyelinating Diseases / Q000628:therapy*; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D009836:Oligodendroglia; D033581:Stem Cell Transplantation / Q000379:methods*
keywords: direct conversion; direct reprogramming; iPSC reprogramming; oligodendrocytes; transdifferentiation