title: Ether-linked lipids of Dermabacter hominis, a human skin actinobacterium.
abstract: Dermabacter hominis is a medically important actinobacterial inhabitant of human skin, although it is rarely implicated in infections. The lipid composition of D. hominis is revisited in this study in the context of its natural resistance to daptomycin, an antibiotic whose activity is influenced by membrane lipids. Thin layer chromatography and mass spectrometry revealed that this species contains phospholipids and glycolipids. Using electrospray ionization time of flight mass spectrometry (exact mass) and gas chromatography-mass spectrometry, the major phospholipid of D. hominis was identified as plasmanyl-phosphatidylglycerol (pPG), because it presented one alkyl chain and one acyl chain in the glycerol moiety of the molecule. The structure of the major glycolipid (GL1) was studied by combined gas-liquid chromatography, mass spectrometry and nuclear magnetic resonance, and was established as galactosyl-α-(1→2)-glucosyl-alkyl-acyl-glycerol. Lipid analyses showed differences between one daptomycin-resistant (DAP-R) strain and one daptomycin-sensitive (DAP-S) strain growing in the presence of the antibiotic: DAP-R tended to accumulate GL1 and to reduce pPG, whereas DAP-S maintained high proportions of pPG. The results demonstrate the existence of ether-linked lipids in D. hominis and reveal a differential distribution of phospholipids and glycolipids according to the sensitivity or resistance to daptomycin, although the mechanism(s) operating in the resistance to the antibiotic remain(s) to be elucidated.
authors: Departamento de Genética y Microbiología, Facultad de Medicina, Universidad de Murcia, Spain; Instituto Murciano de Investigación Biosanitaria (IMIB), Spain. Electronic address: plvalero@um.es.
authors: Pedro L
authors: PL
authors: Valero-Guillén
authors: Departamento de Microbiología Clínica, Complejo Asistencial Universitario de León-Sacyl, León, Spain; Instituto de Biomedicina (IBIOMED), Universidad de León, León, Spain.
authors: Isabel
authors: I
authors: Fernández-Natal
authors: Departamento de Microbiología Clínica, Complejo Asistencial Universitario de León-Sacyl, León, Spain.
authors: Teresa
authors: T
authors: Marrodán-Ciordia
authors: Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie (CeBiTec), Universität Bielefeld, Bielefeld, Germany.
authors: Andreas
authors: A
authors: Tauch
authors: Salud Pública, Escuela de Fisioterapia ONCE, Madrid, Spain.
authors: Francisco
authors: F
authors: Soriano
mesh_terms: D039903:Actinobacteria / Q000737:chemistry* / Q000302:isolation & purification; D004987:Ethers / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D012867:Skin / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004987:Ethers; D008055:Lipids
keywords: Daptomycin; Dermabacter hominis; Ether-linked lipid; Galactosyl-glucosyl-alkyl-acyl-glycerol; Plasmanyl-phosphatidylglycerol