title: Stimulatory effect of the secretogranin-ll derived peptide secretoneurin on food intake and locomotion in female goldfish (Carassius auratus).
abstract: Secretoneurin (SN) is a conserved peptide derived by proteolytic processing from the middle domain of the ∼600 amino acid precursor secretogranin-II (SgII). Secretoneurin is widely distributed in secretory granules of endocrine cells and neurons and has important roles in reproduction as it stimulates luteinizing hormone release from the pituitary. A potential new role of SN in goldfish feeding is the subject of this study. Firstly, we established that acute (26 h; p<0.0001) and short-term (72 h; p=0.016) fasting increased SgIIa precursor mRNA levels 1.25-fold in the telencephalon, implicating SN in the control of feeding. Secondly, we determined that intracerebroventricular injections of the type A SN (SNa; 0.2 and 1 ng/g BW) increased food intake and locomotor behavior by 60 min. Fish injected with the lower and higher doses of SNa (0.2 and 1 ng/g) respectively exhibited significant 1.77- and 2.58-fold higher food intake (p<0.0001) than the saline-injected control fish. Locomotor behavior was increased by 1.35- and 2.26-fold for 0.2 ng/g SNa (p=0.0001) and 1 ng/g SNa (p<0.0001), respectively. Injection of 1 ng/g SNa increased mRNA levels of hypothalamic neuropeptide Y 1.36-fold (p=0.038) and decreased hypothalamic cocaine-and amphetamine-regulated transcript by 33% (p=0.01) at 2h and 5h post-injection, respectively. These data suggest interactions of SNa with stimulatory and inhibitory pathways of food intake control in fish.
authors: Centre of Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, ON, Canada.
authors: M
authors: M
authors: Mikwar
authors: Centre of Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, ON, Canada.
authors: L
authors: L
authors: Navarro-Martin
authors: Centre of Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, ON, Canada.
authors: L
authors: L
authors: Xing
authors: Department of Biology, Memorial University of Newfoundland, St John's, NL, Canada.
authors: H
authors: H
authors: Volkoff
authors: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
authors: W
authors: W
authors: Hu
authors: Centre of Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, ON, Canada. Electronic address: trudeauv@uottawa.ca.
authors: V L
authors: VL
authors: Trudeau
mesh_terms: D000818:Animals; D004435:Eating / Q000187:drug effects* / Q000502:physiology; D005215:Fasting / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006054:Goldfish; D007031:Hypothalamus / Q000187:drug effects* / Q000502:physiology; D007276:Injections, Intraventricular; D008124:Locomotion / Q000187:drug effects* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053381:Secretogranin II / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction; D013238:Stereotaxic Techniques; D013687:Telencephalon / Q000187:drug effects* / Q000502:physiology; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
chemical_list: D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger; D053381:Secretogranin II; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; C081159:secretoneurin
keywords: Feeding; Fish; Locomotion; Secretogranin-II; Secretoneurin