title: Development of oligonucleotide microarrays for simultaneous multi-species identification of Phellinus tree-pathogenic fungi.
abstract: Polyporoid Phellinus fungi are ubiquitously present in the environment and play an important role in shaping forest ecology. Several species of Phellinus are notorious pathogens that can affect a broad variety of tree species in forest, plantation, orchard and urban habitats; however, current detection methods are overly complex and lack the sensitivity required to identify these pathogens at the species level in a timely fashion for effective infestation control. Here, we describe eight oligonucleotide microarray platforms for the simultaneous and specific detection of 17 important Phellinus species, using probes generated from the internal transcribed spacer regions unique to each species. The sensitivity, robustness and efficiency of this Phellinus microarray system was subsequently confirmed against template DNA from two key Phellinus species, as well as field samples collected from tree roots, trunks and surrounding soil. This system can provide early, specific and convenient detection of Phellinus species for forestry, arboriculture and quarantine inspection, and could potentially help to mitigate the environmental and economic impact of Phellinus-related diseases.
authors: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
authors: Yuh
authors: Y
authors: Tzean
authors: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
authors: Po-Yao
authors: PY
authors: Shu
authors: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
authors: Ruey-Fen
authors: RF
authors: Liou
authors: Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
authors: Shean-Shong
authors: SS
authors: Tzean
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D012680:Sensitivity and Specificity; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D015345:Oligonucleotide Probes