title: New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cells.
abstract: FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from consensus on this question. We utilized single-molecule localization microscopy (SMLM) in combination with immunofluorescence staining to visualize FtsZ in Esherichia coli fixed cells that were grown under slow and fast growth conditions. This approach allowed us to obtain images of FtsZ structures at different stages of cell division and accurately measure Z-ring dimensions. Analysis of these images demonstrated that Z-ring thickness increases during constriction, starting at about 70 nm at the beginning of division and increasing by approximately 25% half-way through constriction.
authors: Institute of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya str. 29, Saint Petersburg, 195251, Russia.
authors: Alexey D
authors: AD
authors: Vedyaykin
authors: Institute of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya str. 29, Saint Petersburg, 195251, Russia.|Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av. 4, Saint Petersburg, 194064, Russia.
authors: Innokentii E
authors: IE
authors: Vishnyakov
authors: Institute of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya str. 29, Saint Petersburg, 195251, Russia.
authors: Vasilisa S
authors: VS
authors: Polinovskaya
authors: Institute of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya str. 29, Saint Petersburg, 195251, Russia.
authors: Mikhail A
authors: MA
authors: Khodorkovskii
authors: Institute of Nanobiotechnologies, Peter the Great St. Petersburg Polytechnic University, Polytechnicheskaya str. 29, Saint Petersburg, 195251, Russia.
authors: Anton V
authors: AV
authors: Sabantsev
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002455:Cell Division / Q000235:genetics* / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D019084:Fluorescent Antibody Technique, Indirect; D008856:Microscopy, Fluorescence; D014404:Tubulin / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D014404:Tubulin
keywords: Divisome; FtsZ; Z-ring; single-molecule localization microscopy