title: Oxidative stress and antioxidative systems: recipes for successful data collection and interpretation.
abstract: Oxidative stress and reactive oxygen species (ROS) are common to many fundamental responses of plants. Enormous and ever-growing interest has focused on this research area, leading to an extensive literature that documents the tremendous progress made in recent years. As in other areas of plant biology, advances have been greatly facilitated by developments in genomics-dependent technologies and the application of interdisciplinary techniques that generate information at multiple levels. At the same time, advances in understanding ROS are fundamentally reliant on the use of biochemical and cell biology techniques that are specific to the study of oxidative stress. It is therefore timely to revisit these approaches with the aim of providing a guide to convenient methods and assisting interested researchers in avoiding potential pitfalls. Our critical overview of currently popular methodologies includes a detailed discussion of approaches used to generate oxidative stress, measurements of ROS themselves, determination of major antioxidant metabolites, assays of antioxidative enzymes and marker transcripts for oxidative stress. We consider the applicability of metabolomics, proteomics and transcriptomics approaches and discuss markers such as damage to DNA and RNA. Our discussion of current methodologies is firmly anchored to future technological developments within this popular research field.
authors: Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Bâtiment 630, 91405, Orsay, France.|Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France.
authors: Graham
authors: G
authors: Noctor
authors: Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Bâtiment 630, 91405, Orsay, France.|Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France.|Department of Plant Biotechnology and Bioinformatics, Ghent University, VIB, Department of Plant Systems Biology, Technologie Park 927, B-9052, Ghent, Belgium.
authors: Amna
authors: A
authors: Mhamdi
authors: Centre for Plant Sciences, School of Biology and Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
authors: Christine H
authors: CH
authors: Foyer
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D003625:Data Collection; D055432:Metabolomics; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species
keywords: DNA damage; NAD(P); antioxidative enzymes; ascorbate; glutathione; hydrogen peroxide; lipid peroxides; reactive oxygen species (ROS); redox proteomics