title: [Morphology of collagen matrices for tissue engineering (biocompatibility, biodegradation, tissue response)].
abstract: OBJECTIVE
  to perform a comparative morphological study of biocompatibility, biodegradation, and tissue response to implantation of collagen matrices (scaffolds) for tissue engineering in urology and other areas of medicine.
  
  
  METHODS
  Nine matrix types, such as porous materials reconstructed from collagen solution; a collagen sponge-vicryl mesh composite; decellularized and freeze-dried bovine, equine, and fish dermis; small intestinal submucosa, decellularized bovine dura mater; and decellularized human femoral artery, were implanted subcutaneously in 225 rats. The tissues at the implantation site were investigated for a period of 5 to 90 days. Classical histology and nonlinear optical microscopy (NLOM) were applied.
  
  
  RESULTS
  The investigations showed no rejection of all the collagen materials. The period of matrix bioresorption varied from 10 days for collagen sponges to 2 months for decellularized and freeze-dried vessels and vicryl meshes. Collagen was prone to macrophage resorption and enzymatic lysis, being replaced by granulation tissue and then fibrous tissue, followed by its involution. NLOM allowed the investigators to study the number, density, interposition, and spatial organization of collagen structures in the matrices and adjacent tissues, and their change over time during implantation.
  
  
  CONCLUSIONS
  The performed investigation could recommend three matrices: hybrid collagen/vicryl composite; decellularized bovine dermis; and decellularized porcine small intestinal submucosa, which are most adequate for tissue engineering in urology. These and other collagen matrices may be used in different areas of regenerative medicine.
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: A B
authors: AB
authors: Shekhter
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia; Macquarie University, Sydney, Australia.
authors: A E
authors: AE
authors: Guller
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: L P
authors: LP
authors: Istranov
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: E V
authors: EV
authors: Istranova
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: D V
authors: DV
authors: Butnaru
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: A Z
authors: AZ
authors: Vinarov
authors: Institute for Problems of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
authors: O L
authors: OL
authors: Zakharkina
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: A V
authors: AV
authors: Kurkov
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: D F
authors: DF
authors: Kantimerov
authors: Institute for Problems of Laser and Information Technologies, Russian Academy of Sciences, Moscow, Russia.
authors: E N
authors: EN
authors: Antonov
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: L V
authors: LV
authors: Marisov
authors: I.M. Sechenov First Moscow State Medical University, Moscow, Russia.
authors: P V
authors: PV
authors: Glybochko
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D003094:Collagen / Q000627:therapeutic use* / Q000648:ultrastructure; D005399:Fishes; D006736:Horses; D006801:Humans; D019736:Prostheses and Implants; D051381:Rats; D044968:Regenerative Medicine; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003094:Collagen