title: Surveillance of dengue and chikungunya infection in Dong Thap, Vietnam: A 13-month study.
abstract: OBJECTIVE
  To establish a surveillance in Dong Thap, at the border with Cambodia by assessing the presence of DENV serotypes and CHIKV among patients hospitalized at Dong Thap general hospital.
  
  
  METHODS
  Cross-sectional descriptive analysis was conducted on a cohort of 131 patients hospitalized with acute fever and symptoms compatible with dengue or chikungunya. The study was conducted from January 2012 to February 2013. The full clinical picture was established as well as serological and molecular detection. Serological analysis was sequentially performed on blood samples collected on admission and an average of seven days after admission. The detection of IgM antibody to DENV was performed by IgM capture ELISA and the detection of DENV and CHIKV RNA was done by reverse-transcription multiplex PCR.
  
  
  RESULTS
  101 patients out of 131 (77%) were confirmed with dengue. All four dengue serotypes were detected with a predominance of DENV2 and DENV4. No chikungunya infection was detected although reported in neighboring Cambodia. A differential efficiency of serological dengue detection was observed. Efficiency was 29% upon admission and 53% after seven days on the same patients. 30 patients out of 131 (23%) were negative with both DENV and CHIKV.
  
  
  CONCLUSIONS
  Dengue is at risk of being underestimated and chikungunya is not systematically detected. Changes in detection and surveillance procedures are therefore discussed to increase efficiency of dengue detection and continue the monitoring the emergence of CHIKV in Dong Thap province and in Vietnam.
authors: National Institute of Hygiene and Epidemiology, 1 Yersin Street, 10000 Hanoi, Viet Nam; University of Montpellier, CEMIPAI-CPBS, UMR 5236, 1919, route de Mende, 34293 Montpellier Cedex 5, France.
authors: Pham Thi
authors: PT
authors: Kim Lien
authors: University of Montpellier, CEMIPAI-CPBS, UMR 5236, 1919, route de Mende, 34293 Montpellier Cedex 5, France.
authors: Laurence
authors: L
authors: Briant
authors: Department of Biology, Dong Thap General Hospital, 144, Mai Van Khai, My Xuan, 870000 Cao Lanh, Dong Thap, Viet Nam.
authors: Truong Ba
authors: TB
authors: Tang
authors: National Institute of Hygiene and Epidemiology, 1 Yersin Street, 10000 Hanoi, Viet Nam.
authors: Bui Minh
authors: BM
authors: Trang
authors: University of Montpellier, ISEM, CC063, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
authors: Laurent
authors: L
authors: Gavotte
authors: University of Montpellier, CEMIPAI-CPBS, UMR 5236, 1919, route de Mende, 34293 Montpellier Cedex 5, France.
authors: Emmanuel
authors: E
authors: Cornillot
authors: National Institute of Hygiene and Epidemiology, 1 Yersin Street, 10000 Hanoi, Viet Nam.
authors: Vu Trong
authors: VT
authors: Duoc
authors: National Institute of Hygiene and Epidemiology, 1 Yersin Street, 10000 Hanoi, Viet Nam.
authors: Tran Nhu
authors: TN
authors: Duong
authors: University of Montpellier, CEMIPAI-CPBS, UMR 5236, 1919, route de Mende, 34293 Montpellier Cedex 5, France; Cirad, TA-A17/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France. Electronic address: roger.frutos@univ-montp2.fr.
authors: Roger
authors: R
authors: Frutos
authors: National Institute of Hygiene and Epidemiology, 1 Yersin Street, 10000 Hanoi, Viet Nam. Electronic address: frutossmt@gmail.com.
authors: Phan Thi
authors: PT
authors: Nga
keywords: Arbovirus; Chikungunya; Dengue; Dong Thap; Vietnam