title: Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics.
abstract: The sea cucumber Apostichopus japonicus is exploited as a commercial species owing to their high nutritive and medicinal value. Recent high summer temperatures have caused high mortality rates in A. japonicus. In this study, we applied the isobaric tag for relative and absolute quantitation (iTRAQ) technique to investigate the global protein expression profile under an acute short-term (48 h) heat stress. In total, 3432 proteins were identified, and 127 proteins showed significant heat stress responses, with 61 upregulated proteins and 66 downregulated proteins. Our results suggest that heat stress influenced the expression of proteins involved in various biological processes, such as tissue protection and detoxification, lipid and amino acid metabolism, energy production and usage, transcription and translation, cell apoptosis, and cell proliferation. These findings provide a better understanding about the response and thermo-tolerance mechanisms of A. japonicus under heat stress.
authors: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. dongxuexu1989@126.com.|University of Chinese Academy of Sciences, Beijing 100049, China. dongxuexu1989@126.com.
authors: Dongxue
authors: D
authors: Xu
authors: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. sunlina713@163.com.
authors: Lina
authors: L
authors: Sun
authors: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. shlliu72@126.com.
authors: Shilin
authors: S
authors: Liu
authors: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. zhanglibin@qdio.ac.cn.
authors: Libin
authors: L
authors: Zhang
authors: Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. hshyang@ms.qdio.ac.cn.
authors: Hongsheng
authors: H
authors: Yang
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D018869:Heat-Shock Response; D006358:Hot Temperature; D058977:Molecular Sequence Annotation; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D047528:Stichopus / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D020543:Proteome
keywords: Apostichopus japonicus; heat stress; iTRAQ; proteomics analysis