title: MiR-15a contributes abnormal immune response in myasthenia gravis by targeting CXCL10.
abstract: MiR-15a is likely to be associated with autoimmunity. Here, we aimed to examine the expression of miR-15 cluster in PBMCs from myasthenia gravis (MG) patients and investigate the potential roles of miR-15a in MG. We found that the expression of all miR-15 cluster was decreased in MG, furthermore, miR-15a levels in ocular MG (oMG) were much lower, while CXCL10 production was increased in MG. We display that CXCL10 was a functional target gene of miR-15a in MG. Increasing miR-15a expression could reduce CXCL10 expression and alleviate the abnormal T cells activation in immune response, while decreasing miR-15a expression could activate immune response abnormally. Moreover, miR-15a expression was significantly decreased after stimulation, and prednisone treatment could upregulate miR-15a expression in steroid-responsive MG patients. Take together, our data suggest that decreased miR-15a expression facilitates proinflammatory cytokines production and contributes to immune response at least in part via regulating CXCL10 expression in MG.
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Xiao-Fang
authors: XF
authors: Liu
authors: Department of Pediatrics, The first affiliation hospital of Guangxi Medical University, People's Republic of China.
authors: Run-Qi
authors: RQ
authors: Wang
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Bo
authors: B
authors: Hu
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Meng-Chuan
authors: MC
authors: Luo
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Qiu-Ming
authors: QM
authors: Zeng
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Hao
authors: H
authors: Zhou
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Kun
authors: K
authors: Huang
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Xiao-Hua
authors: XH
authors: Dong
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China.
authors: Yue-Bei
authors: YB
authors: Luo
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China. Electronic address: luozhaohui_xy@126.com.
authors: Zhao-Hui
authors: ZH
authors: Luo
authors: Department of Neurology, Xiangya Hospital, Central South University, People's Republic of China. Electronic address: yangh69@126.com.
authors: Huan
authors: H
authors: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000235:genetics / Q000276:immunology*; D002648:Child; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D009157:Myasthenia Gravis / Q000235:genetics / Q000276:immunology*; D055815:Young Adult
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; C505361:MIRN15 microRNA, human; D035683:MicroRNAs
keywords: CXCL10; Immune response, T cells; Myasthenia gravis; miR-15a