title: [Genetic diversity of Francisella tularensis subsp. holarctica in China].
abstract: OBJECTIVE
  To explore the genetic relationship between the Chinese and the foreign species of Francisella tularensis.
  
  
  METHODS
  Based on our own findings and from the literature, 17 SNP, 4 INDEL, and 12 VNTR were selected for phylogenetic analysis on 39 strains of F.tularensis, including 10 strains of Chinese F. tularensis and 29 strains of foreign F. tularensis that had been sequenced and published. SNP-INDEL and MLVA were used for the separation and combination.
  
  
  RESULTS
  Data from the combined analysis indicated that 3 strains of Chinese F. tularensis with Japanese FSC022 were assigned to B5; 3 strains, with Swedish FSC200 to B1; 3 strains with American OSU18 to B2 and 1 strain with French FTNF002-00, German F92, and American OR96246 to B4, respectively. 10 strains of Chinese F. tularensis were assigned to 4 clades and the result demonstrated a wide diversity of F. tularensis subsp.holarctica in China.
  
  
  CONCLUSIONS
  A set of simple and robust typing tools for F. tularensis subsp.holarctica were established in this study. Based on the results, F. tularensis subsp.holarctica might have had its origins in Asia.
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Email: wangyanhua@icdc.cn.
authors: Yanhua
authors: Y
authors: Wang
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors: Yao
authors: Y
authors: Peng
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors: Lianxu
authors: L
authors: Xia
mesh_terms: D001483:Base Sequence; D002681:China; D005604:Francisella tularensis / Q000235:genetics*; D014644:Genetic Variation; D010802:Phylogeny