title: Three-dimensional bioprinting of multilayered constructs containing human mesenchymal stromal cells for osteochondral tissue regeneration in the rabbit knee joint.
abstract: The use of cell-rich hydrogels for three-dimensional (3D) cell culture has shown great potential for a variety of biomedical applications. However, the fabrication of appropriate constructs has been challenging. In this study, we describe a 3D printing process for the preparation of a multilayered 3D construct containing human mesenchymal stromal cells with a hydrogel comprised of atelocollagen and supramolecular hyaluronic acid (HA). This construct showed outstanding regenerative ability for the reconstruction of an osteochondral tissue in the knee joints of rabbits. We found that the use of a mechanically stable, host-guest chemistry-based hydrogel was essential and allowed two different types of extracellular matrix (ECM) hydrogels to be easily printed and stacked into one multilayered construct without requiring the use of potentially harmful chemical reagents or physical stimuli for post-crosslinking. To the best of our knowledge, this is the first study to validate the potential of a 3D printed multilayered construct consisting of two different ECM materials (atelocollagen and HA) for heterogeneous tissue regeneration using an in vivo animal model. We believe that this 3D printing-based platform technology can be effectively exploited for regeneration of various heterogeneous tissues as well as osteochondral tissue.
authors: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Kyungbuk, Korea. Department of Mechanical Engineering, Korea Polytechnic University, Siheung-si, Gyeonggi-do, Korea.
authors: Jin-Hyung
authors: JH
authors: Shim
authors: Ki-Mo
authors: KM
authors: Jang
authors: Sei Kwang
authors: SK
authors: Hahn
authors: Ju Young
authors: JY
authors: Park
authors: Hyuntae
authors: H
authors: Jung
authors: Kyunghoon
authors: K
authors: Oh
authors: Kyeng Min
authors: KM
authors: Park
authors: Junseok
authors: J
authors: Yeom
authors: Sun Hwa
authors: SH
authors: Park
authors: Sung Won
authors: SW
authors: Kim
authors: Joon Ho
authors: JH
authors: Wang
authors: Kimoon
authors: K
authors: Kim
authors: Dong-Woo
authors: DW
authors: Cho
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D002358:Cartilage, Articular / Q000254:growth & development* / Q000473:pathology; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007719:Knee Joint / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D020370:Osteoarthritis, Knee / Q000473:pathology / Q000628:therapy*; D066330:Printing, Three-Dimensional; D011817:Rabbits; D016896:Treatment Outcome
chemical_list: D020100:Hydrogels