title: Characterization of a novel Kazal-type serine proteinase inhibitor of Arabidopsis thaliana.
abstract: Many different types of serine proteinase inhibitors have been involved in several kinds of plant physiological processes, including defense mechanisms against phytopathogens. Kazal-type serine proteinase inhibitors, which are included in the serine proteinase inhibitor family, are present in several organisms. These proteins play a regulatory role in processes that involve serine proteinases like trypsin, chymotrypsin, thrombin, elastase and/or subtilisin. In the present work, we characterized two putative Kazal-type serine proteinase inhibitors from Arabidopsis thaliana, which have a single putative Kazal-type domain. The expression of these inhibitors is transiently induced in response to leaf infection by Botrytis cinerea, suggesting that they play some role in defense against pathogens. We also evaluated the inhibitory specificity of one of the Kazal-type serine proteinase inhibitors, which resulted to be induced during the local response to B. cinerea infection. The recombinant Kazal-type serine proteinase inhibitor displayed high specificity for elastase and subtilisin, but low specificity for trypsin, suggesting differences in its selectivity. In addition, this inhibitor exhibited a strong antifungal activity inhibiting the germination rate of B. cinerea conidia in vitro. Due to the important role of proteinase inhibitors in plant protection against pathogens and pests, the information about Kazal-type proteinase inhibitors described in the present work could contribute to improving current methods for plant protection against pathogens.
authors: Unidad de Biotecnología 6-UB6, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
authors: Sebastián
authors: S
authors: Pariani
authors: Unidad de Biotecnología 6-UB6, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
authors: Marisol
authors: M
authors: Contreras
authors: Unidad de Biotecnología 3-UB3, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
authors: Franco R
authors: FR
authors: Rossi
authors: Unidad de Biotecnología 6-UB6, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
authors: Valeria
authors: V
authors: Sander
authors: Unidad de Biotecnología 6-UB6, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
authors: Mariana G
authors: MG
authors: Corigliano
authors: Unidad de Biotecnología 6-UB6, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
authors: Francisco
authors: F
authors: Simón
authors: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Rosario, Argentina.
authors: María V
authors: MV
authors: Busi
authors: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Rosario, Argentina.
authors: Diego F
authors: DF
authors: Gomez-Casati
authors: Unidad de Biotecnología 3-UB3, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina.
authors: Fernando L
authors: FL
authors: Pieckenstain
authors: Instituto Nacional de Parasitología "Dr Mario Fatala Chaben", ANLIS-Malbrán, Ministerio de Salud, Buenos Aires, Argentina.
authors: Vilma G
authors: VG
authors: Duschak
authors: Unidad de Biotecnología 6-UB6, IIB-INTECH, CONICET-UNSAM, Chascomús, Argentina. Electronic address: mclemente@intech.gov.ar.
authors: Marina
authors: M
authors: Clemente
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D020171:Botrytis / Q000472:pathogenicity; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors
keywords: Arabidopsis thaliana; Botrytis cinerea; Inhibitory activity; Kazal-type domain; Serine proteinase inhibitor