title: Analysis of differentially expressed proteins in Yersinia enterocolitica-infected HeLa cells.
abstract: Yersinia enterocolitica is a facultative intracellular pathogen and a causative agent of yersiniosis, which can be contracted by ingestion of contaminated food. Yersinia secretes virulence factors to subvert critical pathways in the host cell. In this study we utilized shotgun label-free proteomics to study differential protein expression in epithelial cells infected with Y.enterocolitica. We identified a total of 551 proteins, amongst which 42 were downregulated (including Prostaglandin E Synthase 3, POH-1 and Karyopherin alpha) and 22 were upregulated (including Rab1 and RhoA) in infected cells. We validated some of these results by western blot analysis of proteins extracted from Caco-2 and HeLa cells. The proteomic dataset was used to identify host canonical pathways and molecular functions modulated by this infection in the host cells. This study constitutes a proteome of Yersinia-infected cells and can support new discoveries in the area of host-pathogen interactions.
  
  
  CONCLUSIONS
  We describe a proteome of Yersinia enterocolitica-infected HeLa cells, including a description of specific proteins differentially expressed upon infection, molecular functions as well as pathways altered during infection. This proteomic study can lead to a better understanding of Y. enterocolitica pathogenesis in human epithelial cells.
authors: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA.
authors: Navatha
authors: N
authors: Alugubelly
authors: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA.
authors: Kamil
authors: K
authors: Hercik
authors: Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA.
authors: Peter
authors: P
authors: Kibler
authors: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA.
authors: Bindu
authors: B
authors: Nanduri
authors: Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, USA. Electronic address: medelmann@ufl.edu.
authors: Mariola J
authors: MJ
authors: Edelmann
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D014176:Protein Biosynthesis / Q000235:genetics*; D040901:Proteomics; D015009:Yersinia Infections / Q000235:genetics* / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000472:pathogenicity
keywords: Integrin signaling; Label-free proteomics; Pathway modeling; Protein ubiquitination; Yersinia enterocolitica infection