title: Study of total dry matter and protein extraction from canola meal as affected by the pH, salt addition and use of zeta-potential/turbidimetry analysis to optimize the extraction conditions.
abstract: Total dry matter and proteins were differentially and preferentially extracted from canola meal (CM) under different conditions. The effect of the extraction medium pH, CM concentration and salt concentrations were found to have different influences on the extractability of total dry matter and proteins from CM. The pH of the extracting medium had the most significant effect. The maximal total dry matter (42.8±1.18%) extractability was obtained with 5% CM at pH 12 without salt addition, whereas the maximal for total protein (58.12±1.47%) was obtained with 15% CM under the same conditions. The minimal extractability for the dry matter (26.63±0.67%) was obtained with 5% CM at pH 10 without salt added and the minimal protein extractability was observed in a 10% CM at pH 10, in 0.01 NaCl. Turbidity and ζ-potential measurements indicated that pH 5 was the optimum condition for the highest protein extraction yield. SDS-PAGE analysis showed that salt addition contributes to higher solubility of canola proteins specifically cruciferin fraction, although it reduces napin extraction.
authors: Department of Food Sciences and Nutrition, Université Laval, Quebec, QC G1V 0A6, Canada; Institute of Nutrition and Functional Foods (INAF), Université Laval, Quebec, QC G1V 0A6, Canada.
authors: Alina
authors: A
authors: Gerzhova
authors: Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec J2S 8E3, Canada.
authors: Martin
authors: M
authors: Mondor
authors: Natural Resources Canada/CanmetENERGY, 1615 Lionel-Boulet Blvd., P.O. Box 4800, Varennes, Quebec J3X 1S6, Canada.
authors: Marzouk
authors: M
authors: Benali
authors: Institute of Nutrition and Functional Foods (INAF), Université Laval, Quebec, QC G1V 0A6, Canada; Department of Soil Sciences and Agri-Food Engineering, Université Laval, Quebec, QC G1V 0A6, Canada. Electronic address: mohammed.aider@fsaa.ulaval.ca.
authors: Mohammed
authors: M
authors: Aider
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D000074262:Rapeseed Oil
chemical_list: D005229:Fatty Acids, Monounsaturated; D011506:Proteins; D000074262:Rapeseed Oil
keywords: Canola meal; Optimization; Protein extraction; Turbidity; Zeta potential