title: Description of Leucobacter holotrichiae sp. nov., isolated from the gut of Holotrichia oblita larvae.
abstract: A Gram-stain-positive bacterium, designated T14T, was isolated from the gut of Holotrichia oblita larvae and was subjected to a taxonomic study. The isolate was rod-shaped, aerobic, non-motile, non-spore-forming and yellow-pigmented. Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that the isolate is related to the genus Leucobacter. Its closest neighbours were the type strains 'Leucobacter kyeonggiensis' F3-P9 (96.8% 16S rRNA gene sequence similarity), Leucobacter celer NAL101T (96.2%) and Leucobacter chironomi DSM 19883T (95.5%). The DNA G+C content of strain T14T was 69.3 mol%, and DNA-DNA hybridization values with closely related strains were <32%. The predominant cellular fatty acids were anteiso-C15:0 (49.3%), iso-C16:0 (16.4%) and anteiso-C17:0 (16.8%). The major polar lipids were aminolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphoglycolipid and unidentified glycolipids. The predominant respiratory quinone was MK-11. Based on these phylogenetic and phenotypic results, strain T14T can be clearly distinguished from all of the recognized species of the genus Leucobacter and is considered to represent a novel species of the genus Leucobacter. The name Leucobacter holotrichiae sp. nov. is proposed, with the type strain T14T (=DSM 28968T=JCM 30245T).
authors: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PRChina.
authors: Daochen
authors: D
authors: Zhu
authors: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PRChina.
authors: Peipei
authors: P
authors: Zhang
authors: Institute of Agricultural Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PRChina.
authors: Pingping
authors: P
authors: Li
authors: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PRChina.
authors: Jian
authors: J
authors: Wu
authors: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PRChina.
authors: Changxiao
authors: C
authors: Xie
authors: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PRChina.
authors: Jianzhong
authors: J
authors: Sun
authors: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, PRChina.
authors: Lili
authors: L
authors: Niu
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D001517:Coleoptera / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006017:Glycolipids / Q000737:chemistry; D007814:Larva; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2