title: 786T/c endothelial nitric oxide synthase gene polymorphism and coronary collateral circulation.
abstract: BACKGROUND
  In this study, we investigated the association between -786T/C polymorphism of the endothelial nitric oxide (NOS3) gene in which thymidine is replaced by a cytosine at nucleotide -786 (rs 2070744) and coronary collateral circulation (CCC) in patients with stable coronary artery disease.
  
  
  METHODS
  286 patients having a critical stenosis (> 95%) in at least one major epicardial coronary vessel were included in the study. CCC was defined according to the Rentrop classification (R). Patients with R0-1 CCC were included in the poor CCC group and subjects with R2-3 CCC were assigned to the good CCC group. The polymerase chain reaction method was used for genotyping. 152 patients with poor CCC and 134 patients with good CCC were examined.
  
  
  RESULTS
  The frequency of cytosine-cytosine (CC) and thymidine-cytosine (TC) genotypes and allele C were higher in the poor CCC group, but the difference did not reach statistical significance. In the dominant model, the frequency of CC+TC vs. thymidine-thymidine (TT) genotypes was significantly higher in the poor CCC group (67.1% vs. 54.5%, respectively; χ²=4.78; p=0.02). In multivariate regression analysis, the dominant model for -786T/C polymorphism of the NOS3 gene remained as an independent correlate of poor CCC.
  
  
  CONCLUSIONS
  -786T/C polymorphism of the NOS3 gene (rs 2070744) may be associated with poor angiogenesis and the development of CCC in stable coronary artery disease.
authors: Department of Cardiology, Acıbadem University, School of Medicine, Atakent Hospital, Istanbul, Turkey.
authors: Satilmis
authors: S
authors: Seckin
authors: Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, Training and Research Hospital, Istanbul, Turkey.
authors: Bozbeyoglu
authors: B
authors: Emrah
authors: Department of Cardiology, Usak State Hospital Heart Center, Usak, Turkey.
authors: Ismail
authors: I
authors: Biyik
authors: Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, Training and Research Hospital, Istanbul, Turkey.
authors: Arugaslan
authors: A
authors: Emre
authors: Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, Training and Research Hospital, Istanbul, Turkey.
authors: Tangurek
authors: T
authors: Burak
authors: Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, Training and Research Hospital, Istanbul, Turkey.
authors: Sungur
authors: S
authors: Azmi
authors: Department of Cardiology, Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Training and Research Hospital, Istanbul, Turkey.
authors: Celik
authors: C
authors: Omer
authors: Department of Cardiology, Siyami Ersek Cardiovascular and Thoracic Surgery Center, Training and Research Hospital, Istanbul, Turkey.
authors: Dagdelen
authors: D
authors: Sinan
mesh_terms: D000368:Aged; D000483:Alleles; D003097:Collateral Circulation / Q000235:genetics*; D017023:Coronary Angiography; D003326:Coronary Circulation / Q000235:genetics*; D023921:Coronary Stenosis / Q000000981:diagnostic imaging / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III