title: Silver nanoparticles/chitosan oligosaccharide/poly(vinyl alcohol) nanofiber promotes wound healing by activating TGFβ1/Smad signaling pathway.
abstract: Wound healing occupies a remarkable place in everyday pathology and remains a challenging clinical problem. In our previous study, we prepared a silver nanoparticle/chitosan oligosaccharide/poly(vinyl alcohol) (PVA/COS-AgNPs) nanofiber via electrospinning and revealed that it could promote wound healing; however, the healing mechanism remained unknown. Therefore, we aimed to clarify the mechanism underlying the accelerated healing effect of the PVA/COS-AgNPs nanofiber. The TGFβ1/Smad signaling pathway is actively involved in wound healing. Considering the key role of this signaling pathway in wound healing, our preliminary study showed that the TGFβ1 level was significantly increased during the early stage of wound healing. Thus, in this study, hematoxylin-eosin, Masson's trichrome, immunofluorescent staining, hydroxyproline content, quantitative real-time polymerase chain reaction, and Western blot analyses were used to analyze the wound healing in a rat model treated with gauze, the PVA/COS-AgNPs nanofiber, and the nanofiber plus SB431542 (an inhibitor of TGFβ1 receptor kinase). The results showed that the PVA/COS-AgNPs nanofiber promoted wound healing and upregulated the expression levels of cytokines associated with the TGFβ1/Smad signaling pathway such as TGFβ1, TGFβRI, TGFβRII, collagen I, collagen III, pSmad2, and pSmad3. Inhibiting this pathway with SB431542 resulted in prevention of the PVA/COS-AgNPs nanofiber-associated salutary effects on the early stage of wound healing and relative cytokines expression. In conclusion, the wound healing effect of the PVA/COS-AgNPs nanofiber involves activation of the TGFβ1/Smad signaling pathway.
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Chen-wen
authors: CW
authors: Li
authors: College of Pharmacy, Chongqing Medical University, Chongqing, People's Republic of China.
authors: Qing
authors: Q
authors: Wang
authors: Department of Pharmacy, the Affiliated Hospital of Qingdao University, Qingdao, People's Republic of China.
authors: Jing
authors: J
authors: Li
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Min
authors: M
authors: Hu
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: San-jun
authors: SJ
authors: Shi
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Zi-wei
authors: ZW
authors: Li
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Guo-lin
authors: GL
authors: Wu
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Huan-huan
authors: HH
authors: Cui
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Yuan-yuan
authors: YY
authors: Li
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Qian
authors: Q
authors: Zhang
authors: College of Pharmacy, Chongqing Medical University, Chongqing, People's Republic of China.
authors: Xiu-heng
authors: XH
authors: Yu
authors: Department of Pharmacy, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors: Lai-chun
authors: LC
authors: Lu
mesh_terms: D000818:Animals; D001458:Bandages; D015153:Blotting, Western; D048271:Chitosan / Q000737:chemistry*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D057139:Nanofibers / Q000008:administration & dosage* / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D051785:Smad Proteins / Q000235:genetics / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D012333:RNA, Messenger; D051785:Smad Proteins; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D012834:Silver; D048271:Chitosan
keywords: Smad proteins; TGFβ1; electrospinning; nanofiber; silver nanoparticles; wound healing