title: Endosomal Interactions during Root Hair Growth.
abstract: The dynamic localization of endosomal compartments labeled with targeted fluorescent protein tags is routinely followed by time lapse fluorescence microscopy approaches and single particle tracking algorithms. In this way trajectories of individual endosomes can be mapped and linked to physiological processes as cell growth. However, other aspects of dynamic behavior including endosomal interactions are difficult to follow in this manner. Therefore, we characterized the localization and dynamic properties of early and late endosomes throughout the entire course of root hair formation by means of spinning disc time lapse imaging and post-acquisition automated multitracking and quantitative analysis. Our results show differential motile behavior of early and late endosomes and interactions of late endosomes that may be specified to particular root hair domains. Detailed data analysis revealed a particular transient interaction between late endosomes-termed herein as dancing-endosomes-which is not concluding to vesicular fusion. Endosomes preferentially located in the root hair tip interacted as dancing-endosomes and traveled short distances during this interaction. Finally, sizes of early and late endosomes were addressed by means of super-resolution structured illumination microscopy (SIM) to corroborate measurements on the spinning disc. This is a first study providing quantitative microscopic data on dynamic spatio-temporal interactions of endosomes during root hair tip growth.
authors: Department of Plant Cell Biology, Institute of Cellular and Molecular Botany, University of Bonn Bonn, Germany.
authors: Daniel
authors: D
authors: von Wangenheim
authors: Department of Cell Biology, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Czech Republic.
authors: Amparo
authors: A
authors: Rosero
authors: Department of Cell Biology, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Czech Republic.
authors: George
authors: G
authors: Komis
authors: Department of Cell Biology, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Czech Republic.
authors: Olga
authors: O
authors: Šamajová
authors: Department of Cell Biology, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Czech Republic.
authors: Miroslav
authors: M
authors: Ovečka
authors: Department of Plant Cell Biology, Institute of Cellular and Molecular Botany, University of Bonn Bonn, Germany.
authors: Boris
authors: B
authors: Voigt
authors: Department of Cell Biology, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Czech Republic.
authors: Jozef
authors: J
authors: Šamaj
keywords: Arabidopsis thaliana; development; endosomes; interaction; root hair; spinning disc microscopy; structured illumination microscopy; trafficking