title: Immunogenicity of a recombinant Sendai virus expressing the capsid precursor polypeptide of foot-and-mouth disease virus.
abstract: In this study, SeV was used as a vector to express capsid precursor polypeptide (P1) of Foot-and-mouth disease virus (FMDV) by using reverse genetics. The rescue recombinant SeV (rSeV-P1) can efficiently propagate and express P1 protein by Western blot and IFA analysis. To evaluate the immunogenicity of rSeV-P1, BALB/c mice were divided into several groups and immunized intramuscularly with various doses of rSeV-P1, rSeV-eGFP, PBS and commercial FMD vaccine, respectively, and then challenged with an intraperitoneal injection of 1 × 10(6) TCID50 of virulent serotype O FMDV O/ES/2001 strain 4 weeks after booster immunization. Mice vaccinated with rSeV-P1 acquired FMDV-specific ELISA antibodies, neutralizing antibodies as well as cellular immune response. Meantime, mice immunized with rSeV-P1 (dose-dependent) had the ability to inhibit the replication of FMDV in the sera after FMDV challenge. Our results indicated that the recombinant SeV-P1 virus could be utilized as an alternative strategy to develop a new generation of safety and efficacious vaccine against FMDV infection.
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; The Animal Multiplication Farm of Hubei Province, The Hubei Animal Husbandry and Veterinary Bureau, No.69 Xiongchu Ave., Wuchang District, Wuhan 4300064, PR China.
authors: Guo-Ging
authors: GG
authors: Zhang
authors: China Institute of Veterinary Drug Control, No.8 south street of Zhongguancun, Haidian district, Beijing, PR China.
authors: Xiao-Yun
authors: XY
authors: Chen
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Key Laboratory of development of veterinary diagnostic products, Ministry of Agriculture, Wuhan 430070, PR China.
authors: Ping
authors: P
authors: Qian
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Key Laboratory of development of veterinary diagnostic products, Ministry of Agriculture, Wuhan 430070, PR China.
authors: Huan-Chun
authors: HC
authors: Chen
authors: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, PR China; Key Laboratory of development of veterinary diagnostic products, Ministry of Agriculture, Wuhan 430070, PR China. Electronic address: lixiangmin@mail.hzau.edu.cn.
authors: Xiang-Min
authors: XM
authors: Li
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000276:immunology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000517:prevention & control* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D029082:Sendai virus / Q000276:immunology*; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D010455:Peptides; D011994:Recombinant Proteins; D014765:Viral Vaccines
keywords: Capsid precursor polypeptide; FMDV; Immunogenicity; Recombinant SeV-P1