title: Intrinsic features of the CD8α(-) dendritic cell subset in inducing functional T follicular helper cells.
abstract: T follicular helper (Tfh) cells, a true B cell helper, have a critical role in enhancing humoral immune responses. However, the initial differentiation of Tfh cells by dendritic cells (DCs), the most potent antigen presenting cells, has not been clearly understood, particularly in the knowledge of the two major conventional dendritic cell subsets, CD8α(+) DCs or CD8α(-) DCs. Here we demonstrated that the localization of CD8α(-) DCs in the marginal zone (MZ) bridging channels is closely associated with the induction of CXCR5(+)CCR7(low) Tfh cells. We also showed that the major source of IL-6 for inducing Tfh cells is provided from the activated CD4(+) T cells induced by CD8α(-) DCs, and IL-6 directly secreted from the DC subsets seems minor. CD8α(-) DCs were superior in inducing functional Tfh cells over other antigen presenting cells including B cells. We here observed the unknown intrinsic features of the DC subsets, suggesting the potential of utilizing the CD8α(-) DC subset as therapeutic vaccine for the regulation of humoral immune responses.
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-gil 50, Ulsan 44919, Republic of Korea.
authors: Changsik
authors: C
authors: Shin
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-gil 50, Ulsan 44919, Republic of Korea.
authors: Jae-A
authors: JA
authors: Han
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-gil 50, Ulsan 44919, Republic of Korea.
authors: Bongseo
authors: B
authors: Choi
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-gil 50, Ulsan 44919, Republic of Korea; Center for Soft and Living Matter, Institute for Basic Science (IBS), UNIST-gil 50, Ulsan 44919, Republic of Korea.
authors: Yoon-Kyoung
authors: YK
authors: Cho
authors: School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-gil 50, Ulsan 44919, Republic of Korea.
authors: Yoonkyung
authors: Y
authors: Do
authors: Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Chonan-Si, Chungcheongnam-do 31538, Republic of Korea. Electronic address: ryu@sch.ac.kr.
authors: Seongho
authors: S
authors: Ryu
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016827:CD8 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D018858:Germinal Center / Q000276:immunology; D056724:Immunity, Humoral; D016219:Immunotherapy, Adoptive / Q000379:methods*; D015850:Interleukin-6 / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D054400:Receptors, CCR7 / Q000378:metabolism; D054380:Receptors, CXCR5 / Q000378:metabolism
chemical_list: D016827:CD8 Antigens; C431698:CD8alpha antigen; C513179:CXCR5 protein, mouse; C515371:Ccr7 protein, mouse; D015850:Interleukin-6; D054400:Receptors, CCR7; D054380:Receptors, CXCR5
keywords: Cellular immunity; Cytokine; Dendritic cell subset; Humoral immunity; Localization; T follicular helper cell