title: [A New Enzyme Preparation with High Penicillopepsin Activity Based on the Producer Strain Penicillium canescens].
abstract: The producer of fungal penicillopepsin, an aspartate protease, has been created by genetic engineering. The biochemical and physicochemical properties of the penicillopepsin enzyme preparation obtained from the culture liquid of the producer were studied. Properties of the new enzyme preparation and the commercially available aspergillopepsin were compared. Their proteolytic activities were found to be 670-680 U/g of the preparation. The soluble protein yield upon the wheat flour hydrolysis with penicillopepsin was 2.7 times higher than with aspergillopepsin. It is probably caused by the presence of the xylanase activity in the penicillopepsin preparation.
authors: I A
authors: IA
authors: Smirnova
authors: A S
authors: AS
authors: Sereda
authors: E V
authors: EV
authors: Kostyleva
authors: N V
authors: NV
authors: Tsurikova
authors: E V
authors: EV
authors: Bushina
authors: A M
authors: AM
authors: Rozhkova
authors: A P
authors: AP
authors: Sinitsyn
mesh_terms: D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism*; D057055:Aspartic Acid Proteases / Q000235:genetics / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005433:Flour / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005818:Genetic Engineering; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D059748:Proteolysis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D043364:Endo-1,4-beta Xylanases; D057055:Aspartic Acid Proteases; D016282:Aspartic Acid Endopeptidases; C029094:aspergillopepsin I