title: A molecular nanodevice for targeted degradation of mRNA during protein synthesis.
abstract: RNase H is an endonuclease that catalyzes the cleavage of RNA. Because it only acts on RNA in RNA:DNA hybrids, RNase H can be used for targeted degradation of RNA when used in combination with antisense oligodeoxyribonucleotides (ASODNs) designed against a specific sequence of the target RNA. In this study, ASODN and RNase H were co-conjugated on magnetic nanoparticles. The resulting nanoparticles, having integrated functions of probing and processing target RNA, were able to remove target mRNA sequences more effectively than free ASODNs. The paramagnetic property of the nanoparticles also enabled timed engagement and disengagement of the RNA-degrading components in a given system, and these nanoparticles were able to be used for ON/OFF control of gene expression during cell-free protein synthesis reactions.
authors: Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
authors: Kyung-Ho
authors: KH
authors: Lee
authors: Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
authors: Seung-Eui
authors: SE
authors: Min
authors: Synthetic Biology and Bioengineering Research Center, KRIBB, Daejeon 34141, Korea.
authors: Haseong
authors: H
authors: Kim
authors: Synthetic Biology and Bioengineering Research Center, KRIBB, Daejeon 34141, Korea.
authors: Seung-Goo
authors: SG
authors: Lee
authors: Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Korea.
authors: Dong-Myung
authors: DM
authors: Kim
mesh_terms: D002474:Cell-Free System; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D020319:Oligodeoxyribonucleotides, Antisense / Q000737:chemistry*; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000737:chemistry*; D016914:Ribonuclease H / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D016914:Ribonuclease H