title: Cation Homeostasis in Red Cells From Patients With Sickle Cell Disease Heterologous for HbS and HbC (HbSC Genotype).
abstract: Sickle cell disease (SCD) in patients of HbSC genotype is considered similar, albeit milder, to that in homozygous HbSS individuals--but with little justification. In SCD, elevated red cell cation permeability is critical as increased solute loss causes dehydration and encourages sickling. Recently, we showed that the KCl cotransporter (KCC) activity in red cells from HbSC patients correlated significantly with disease severity, but that in HbSS patients did not. Two transporters involved in red cell dehydration, the conductive channels Psickle and the Gardos channel, behaved similarly in red cells from the two genotypes, but were significantly less active in HbSC patients. By contrast, KCC activity was quantitatively greater in HbSC red cells. Results suggest that KCC is likely to have greater involvement in red cell dehydration in HbSC patients, which could explain its association with disease severity in this genotype. This work supports the hypothesis that SCD in HbSC patients is a distinct disease entity to that in HbSS patients. Results suggest the possibility of designing specific treatments of particular benefit to HbSC patients and a rationale for the development of prognostic markers, to inform early treatment of children likely to develop more severe complications of the disease.
authors: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK.
authors: A
authors: A
authors: Hannemann
authors: Department of Paediatric Haematology, King's College London School of Medicine, King's College Hospital NHS Foundation Trust, Denmark Hill, London SE5 9RS, UK.
authors: D C
authors: DC
authors: Rees
authors: Department of Paediatric Haematology, King's College London School of Medicine, King's College Hospital NHS Foundation Trust, Denmark Hill, London SE5 9RS, UK.
authors: S
authors: S
authors: Tewari
authors: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK.
authors: J S
authors: JS
authors: Gibson
mesh_terms: D000755:Anemia, Sickle Cell / Q000235:genetics* / Q000378:metabolism*; D001692:Biological Transport; D002412:Cations / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D004913:Erythrocytes, Abnormal / Q000378:metabolism; D005838:Genotype; D006444:Hemoglobin C / Q000235:genetics*; D006451:Hemoglobin, Sickle / Q000235:genetics*; D006706:Homeostasis; D006801:Humans; D010101:Oxygen Consumption; D011188:Potassium / Q000378:metabolism; D027981:Symporters / Q000378:metabolism; D000096922:K Cl- Cotransporters
chemical_list: D002412:Cations; D006444:Hemoglobin C; D006451:Hemoglobin, Sickle; D011188:Potassium; D027981:Symporters; D000096922:K Cl- Cotransporters
keywords: HbSC; KCl cotransport; Potassium permeability; Red cells; Sickle cell disease