title: Data describing the effects of dietary bioactive agents on colonic stem cell microRNA and mRNA expression.
abstract: With the identification of Lgr5 as a definitive marker for intestinal stem cells, we used the highly novel, recently described, Lgr5-EGFP-IRES-cre ER (T2) knock in mouse model. Mice were injected with azoxymethane (AOM, a colon carcinogen) or saline (control) and fed a chemo-protective diet containing n-3 fatty acids and fermentable fiber (n-3 PUFA+pectin) or a control diet (n-6 PUFA + cellulose). Single cells were isolated from colonic mucosa crypts and three discrete populations of cells were collected via fluorescence activated cell sorting (FACS): Lgr5(high) (stem cells), Lgr5(low) (daughter cells) and Lgr5(negative) (differentiated cells). microRNA profiling and RNA sequencing were performed from the same sample and analyzed. These data refer to 'Comparative effects of diet and carcinogen on microRNA expression in the stem cell niche of the mouse colonic crypt' (Shah et al., 2016) [5].
authors: Program in Integrative Nutrition & Complex Diseases, United States; Intercollegiate Faculty of Genetics, United States; Divison of Endocrinology, Boston Children's Hospital, United States; Department of Pediatrics, Harvard Medical School, Boston, MA, United States.
authors: Manasvi S
authors: MS
authors: Shah
authors: Program in Integrative Nutrition & Complex Diseases, United States; Department of Molecular and Cellular Medicine, United States.
authors: Eunjoo
authors: E
authors: Kim
authors: Program in Integrative Nutrition & Complex Diseases, United States.
authors: Laurie A
authors: LA
authors: Davidson
authors: Program in Integrative Nutrition & Complex Diseases, United States; Department of Electrical Engineering, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
authors: Jason M
authors: JM
authors: Knight
authors: Program in Integrative Nutrition & Complex Diseases, United States; Department of Statistics, United States.
authors: Roger S
authors: RS
authors: Zoh
authors: Program in Integrative Nutrition & Complex Diseases, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
authors: Jennifer S
authors: JS
authors: Goldsby
authors: Program in Integrative Nutrition & Complex Diseases, United States.
authors: Evelyn S
authors: ES
authors: Callaway
authors: Department of Veterinary Physiology & Pharmacology, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
authors: Beyian
authors: B
authors: Zhou
authors: Program in Integrative Nutrition & Complex Diseases, United States; Department of Veterinary Physiology & Pharmacology, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
authors: Ivan
authors: I
authors: Ivanov
authors: Program in Integrative Nutrition & Complex Diseases, United States; Intercollegiate Faculty of Genetics, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
authors: Robert S
authors: RS
authors: Chapkin