title: Molecular modeling of the elastomeric properties of repeating units and building blocks of resilin, a disordered elastic protein.
abstract: The mechanisms responsible for the properties of disordered elastomeric proteins are not well known. To better understand the relationship between elastomeric behavior and amino acid sequence, we investigated resilin, a disordered rubber-like protein, found in specialized regions of the cuticle of insects. Resilin of Drosophila melanogaster contains Gly-rich repetitive motifs comprised of the amino acids, PSSSYGAPGGGNGGR, which confer elastic properties to resilin. The repetitive motifs of insect resilin can be divided into smaller partially conserved building blocks: PSS, SYGAP, GGGN and GGR. Using molecular dynamics (MD) simulations, we studied the relative roles of SYGAP, and its less common variants SYSAP and TYGAP, on the elastomeric properties of resilin. Results showed that SYGAP adopts a bent structure that is one-half to one-third the end-to-end length of the other motifs having an equal number of amino acids but containing SYSAP or TYGAP substituted for SYGAP. The bent structure of SYGAP forms due to conformational freedom of glycine, and hydrogen bonding within the motif apparently plays a role in maintaining this conformation. These structural features of SYGAP result in higher extensibility compared to other motifs, which may contribute to elastic properties at the macroscopic level. Overall, the results are consistent with a role for the SYGAP building block in the elastomeric properties of these disordered proteins. What we learned from simulating the repetitive motifs of resilin may be applicable to the biology and mechanics of other elastomeric biomaterials, and may provide us the deeper understanding of their unique properties.
authors: Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, VA, United States. Electronic address: mkkhanda@vt.edu.
authors: Md Shahriar K
authors: MSK
authors: Khandaker
authors: Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, VA, United States.
authors: Daniel M
authors: DM
authors: Dudek
authors: Horticulture Department, Virginia Polytechnic Institute and State University, United States. Electronic address: ebeers@vt.edu.
authors: Eric P
authors: EP
authors: Beers
authors: Department of Biomedical Engineering & Mechanics, Virginia Polytechnic Institute and State University, United States. Electronic address: dillard@vt.edu.
authors: David A
authors: DA
authors: Dillard
authors: Biochemistry Department, Virginia Polytechnic Institute and State University, United States. Electronic address: drbevan@vt.edu.
authors: David R
authors: DR
authors: Bevan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004331:Drosophila melanogaster; D004548:Elasticity; D019476:Insect Proteins / Q000737:chemistry*; D008958:Models, Molecular
chemical_list: D019476:Insect Proteins; C030951:resilin
keywords: Disordered protein; Elastomeric proteins; Hydrogen bonds; Molecular modeling; Repetitive motifs; Resilin