title: Genetic Interaction between HOG1 and SLT2 Genes in Signalling the Cellular Stress Caused by Sulphuric Acid in Saccharomyces cerevisiae.
abstract: In fuel ethanol production, recycling of yeast biomass includes treatment of cells with diluted sulphuric acid in order to control bacterial population. However, this strategy might lead to a loss of cell viability, with potential negative consequences to the fermentation yield. In a recent paper we showed that the proteins Slt2 and Hog1 are essential for yeast tolerance to sulphuric acid. As a complement of the aforementioned work, we used DNA microarray technology to search for differentially expressed genes in hog1Δ and slt2Δ deletion mutants after treatment with sulphuric acid. Our results show how Slt2p and Hog1p could coordinate the interplay among protein kinase A (PKA), protein kinase C and high-osmolarity glycerol pathways. Moreover, the SSK22 and KDX1 genes may be part of this network, although their proteins were shown to be non-essential for cell growth/survival at low pH. These proteins might work by enhancing the signal which downregulates the PKA pathway leading to cell cycle arrest, in order to regenerate the integrity of yeast cell wall and cell homeostasis under acid shock.
authors: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, Brazil.
authors: Rodrigo Mendonça
authors: RM
authors: de Lucena
authors: Carolina
authors: C
authors: Elsztein
authors: Rafael
authors: R
authors: Barros de Souza
authors: Will
authors: W
authors: de Barros Pita
authors: Sérgio de Sá Leitão
authors: Sde S
authors: Paiva
authors: Marcos Antonio
authors: MA
authors: de Morais
mesh_terms: D000220:Adaptation, Biological; D056804:Biofuels; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D015536:Down-Regulation; D004843:Epistasis, Genetic; D000431:Ethanol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011493:Protein Kinase C / Q000378:metabolism; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D013464:Sulfuric Acids / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D056804:Biofuels; C080988:MLP1 protein, S cerevisiae; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D013464:Sulfuric Acids; D014157:Transcription Factors; D000431:Ethanol; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; C088589:HOG1 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases; C072724:SLT2 protein, S cerevisiae; D020930:MAP Kinase Kinase Kinases; C476806:SSK22 protein, S cerevisiae; D005990:Glycerol