title: Electrochemical simulation of metabolic reactions of the secondary fungal metabolites alternariol and alternariol methyl ether.
abstract: Mycotoxins are secondary plant metabolites that have been found to cause severe diseases in humans and livestock. Exposure can take place on a daily basis since mycotoxins can be found not only in food, animal food, and dietary supplements but also in materials used in buildings. For this work, the Alternaria toxins alternariol (AOH) and alternariol methyl ether (AME) are chosen as representatives for this relevant compound class and are investigated regarding their oxidative phase I metabolism using a combination of electrochemical (EC) oxidation and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). This previously established method has been proven to be a valuable tool for the electrochemical simulation of certain phase I metabolic reactions. A comparison of the electrochemically generated products with those formed during microsomal incubation demonstrates the potential of the method for the successful prediction of the main phase I metabolic reactions of mycotoxins. It can thus find use as a supportive method in the elucidation of the metabolic pathways of various mycotoxins.
authors: Institute of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149, Münster, Germany.
authors: Hannah
authors: H
authors: Simon
authors: Institute of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149, Münster, Germany.
authors: Grete
authors: G
authors: Hoffmann
authors: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstr. 45, 48149, Münster, Germany.
authors: Florian
authors: F
authors: Hübner
authors: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstr. 45, 48149, Münster, Germany.
authors: Hans-Ulrich
authors: HU
authors: Humpf
authors: Institute of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149, Münster, Germany. uk@uni-muenster.de.
authors: Uwe
authors: U
authors: Karst
mesh_terms: D000528:Alternaria / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques / Q000379:methods*; D007783:Lactones / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007783:Lactones; C005197:alternariol
keywords: Alternariol; Alternariol methyl ether; Electrochemistry; Mass Spectrometry; Metabolism