title: Expression of microRNA-133 inhibits epithelial-mesenchymal transition in lung cancer cells by directly targeting FOXQ1.
abstract: BACKGROUND
  MicroRNA (miR) was implicated in the tumorigenesis of many types of cancer, but no study was conducted on the exact role of miR-133 in lung cancer.
  
  
  METHODS
  We have identified miR-133 as a putative regulator of FOXQ1 expression, and investigated the potential involvement of miR-133 in the migration and invasion of lung cancer cells, as well as the underlying molecular mechanism.
  
  
  RESULTS
  MiR-133 directly targeted and down-regulated FOXQ1 expression, which in turn reduced TGF-β level. MiR-133 was down-regulated in lung cancer cell lines A549 and HCC827, and its re-expression significantly inhibited the migration and invasion of the lung cancer cells. Further investigation revealed that this inhibition was caused by reversing the epithelial-mesenchymal transition, evidenced by miR-133 induced elevation of epithelial marker E-cadherin, and reduction of mesenchymal marker Vimentin.
  
  
  CONCLUSIONS
  Our study is the first to identify miR-133 as a biomarker for lung cancer. It functions to down-regulate FOXQ1, and inhibit epithelial-mesenchymal transition, which antagonizes lung cancer tumorigenesis. Therefore our data support the role of miR-133 as a potential molecular therapeutic tool in treating lung cancer.
authors: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu, China; Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, China.
authors: Bo
authors: B
authors: Xiao
authors: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors: Huazhen
authors: H
authors: Liu
authors: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu, China.
authors: Zeyun
authors: Z
authors: Gu
authors: School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu, China. Electronic address: jichenggreen@163.com.
authors: Cheng
authors: C
authors: Ji
mesh_terms: D020413:3' Untranslated Regions; D015703:Antigens, CD; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D014746:Vimentin / Q000096:biosynthesis / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C484579:FOXQ1 protein, human; D051858:Forkhead Transcription Factors; C548234:MIRN133 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D016212:Transforming Growth Factor beta; D014746:Vimentin
keywords: Cáncer de pulmón; Epithelial-mesenchymal transition; FOXQ1; Lung cancer; MicroARN-133; MicroRNA-133; TGF-β; Transición epitelio-mesenquimatosa