title: Tofla virus: A newly identified Nairovirus of the Crimean-Congo hemorrhagic fever group isolated from ticks in Japan.
abstract: Ixodid ticks transmit several important viral pathogens. We isolated a new virus (Tofla virus: TFLV) from Heamaphysalis flava and Heamaphysalis formsensis in Japan. The full-genome sequences revealed that TFLV belonged to the genus Nairovirus, family Bunyaviridae. Phylogenetic analyses and neutralization tests suggested that TFLV is closely related to the Hazara virus and that it is classified into the Crimean-Congo hemorrhagic fever group. TFLV caused lethal infection in IFNAR KO mice. The TFLV-infected mice exhibited a gastrointestinal disorder, and positron emission tomography-computed tomography images showed a significant uptake of (18)F-fluorodeoxyglucose in the intestinal tract. TFLV was able to infect and propagate in cultured cells of African green monkey-derived Vero E6 cells and human-derived SK-N-SH, T98-G and HEK-293 cells. Although TFLV infections in humans and animals are currently unknown, our findings may provide clues to understand the potential infectivity and to develop of pre-emptive countermeasures against this new tick-borne Nairovirus.
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.|Leading graduate school program, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Satoshi
authors: S
authors: Shimada
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Kotaro
authors: K
authors: Aoki
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Takeshi
authors: T
authors: Nabeshima
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Yu
authors: Y
authors: Fuxun
authors: Department of Emerging Infectious Diseases, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Yohei
authors: Y
authors: Kurosaki
authors: Department of Pathology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors: Kazuya
authors: K
authors: Shiogama
authors: Department of Pathology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors: Takanori
authors: T
authors: Onouchi
authors: Central Laboratory, Institute of Tropical Medicine, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Miako
authors: M
authors: Sakaguchi
authors: Department of Hygienic Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
authors: Takeshi
authors: T
authors: Fuchigami
authors: Department of Hygienic Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
authors: Hokuto
authors: H
authors: Ono
authors: Department of Radioisotope Medicine, Atomic Bomb Diseases Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Kodai
authors: K
authors: Nishi
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Guillermo
authors: G
authors: Posadas-Herrera
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Leo
authors: L
authors: Uchida
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Yuki
authors: Y
authors: Takamatsu
authors: Department of Emerging Infectious Diseases, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.|Leading graduate school program, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Jiro
authors: J
authors: Yasuda
authors: Department of Pathology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
authors: Yutaka
authors: Y
authors: Tsutsumi
authors: Mahara Institute of Medical Acarology, 56-3 korekuni Aratano-cho, Anan, Tokushima 779-1510, Japan.
authors: Hiromi
authors: H
authors: Fujita
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.|Leading graduate school program, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Kouichi
authors: K
authors: Morita
authors: Department of Virology, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.|Leading graduate school program, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
authors: Daisuke
authors: D
authors: Hayasaka
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D002044:Bunyaviridae Infections / Q000401:mortality / Q000473:pathology / Q000821:virology*; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D062665:Epidemiological Monitoring; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000473:pathology / Q000821:virology; D016679:Genome, Viral; D057809:HEK293 Cells; D006801:Humans; D007564:Japan; D051379:Mice; D018345:Mice, Knockout; D016855:Nairovirus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D009457:Neuroglia / Q000473:pathology / Q000821:virology; D009474:Neurons / Q000473:pathology / Q000821:virology; D009500:Neutralization Tests; D010802:Phylogeny; D000072078:Positron Emission Tomography Computed Tomography; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency / Q000235:genetics; D017423:Sequence Analysis, RNA; D016019:Survival Analysis; D013987:Ticks / Q000821:virology*; D014709:Vero Cells
chemical_list: C508032:Ifnar1 protein, mouse; D019788:Fluorodeoxyglucose F18; D053633:Receptor, Interferon alpha-beta