title: Manufacturing of AcMNPV baculovirus vectors to enable gene therapy trials.
abstract: Over the past two decades, baculoviruses have become workhorse research tools for transient transgene expression. Although they have not yet been used directly as a gene therapy vector in the clinical setting, numerous preclinical studies have suggested the highly promising potential of baculovirus as a delivery vector for a variety of therapeutic applications including vaccination, tissue engineering, and cancer treatment. As such, there is growing interest in using baculoviruses as human gene therapy vectors, which has led to advances in baculovirus bioprocessing methods. This review provides an overview of the current approaches for scaled-up amplification, concentration, purification, and formulation of AcMNPV baculoviruses, and highlights the key regulatory requirements that must be met before gene therapy clinical trials can be initiated.
authors: Tessa Therapeutics, Pte Ltd. , Singapore.
authors: Timothy Weixin
authors: TW
authors: Kwang
authors: Tessa Therapeutics, Pte Ltd. , Singapore.
authors: Xinhui
authors: X
authors: Zeng
authors: Department of Biological Sciences, National University of Singapore, Singapore; Institute of Bioengineering and Nanotechnology, Singapore.
authors: Shu
authors: S
authors: Wang