title: Ly6G-mediated depletion of neutrophils is dependent on macrophages.
abstract: Antibody-mediated depletion of neutrophils is commonly used to study neutropenia. However, the mechanisms by which antibodies deplete neutrophils have not been well defined. We noticed that mice deficient in complement and macrophages had blunted neutrophil depletion in response to anti-Ly6G monoclonal antibody (MAb) treatment. In vitro, exposure of murine neutrophils to anti-Ly6G MAb in the presence of plasma did not result in significant depletion of cells, either in the presence or absence of complement. In vivo, anti-Ly6G-mediated neutrophil depletion was abrogated following macrophage depletion, but not complement depletion, indicating a requirement for macrophages to induce neutropenia by this method. These results inform the use and limitations of anti-Ly6G antibody as an experimental tool for depleting neutrophils in various immunological settings.
authors: Department of Molecular Microbiology and Immunology, Keck School of Medicine at the University of Southern California (USC), Los Angeles, CA, USA.
authors: Kevin W
authors: KW
authors: Bruhn
authors: Department of Molecular Microbiology and Immunology, Keck School of Medicine at the University of Southern California (USC), Los Angeles, CA, USA.
authors: Ken
authors: K
authors: Dekitani
authors: Department of Molecular Microbiology and Immunology, Keck School of Medicine at the University of Southern California (USC), Los Angeles, CA, USA.
authors: Travis B
authors: TB
authors: Nielsen
authors: Department of Molecular Microbiology and Immunology, Keck School of Medicine at the University of Southern California (USC), Los Angeles, CA, USA.
authors: Paul
authors: P
authors: Pantapalangkoor
authors: Division of Infectious Diseases, Keck School of Medicine at USC, Los Angeles, CA, USA.
authors: Brad
authors: B
authors: Spellberg
keywords: Antibody; CVF, cobra venom factor; Depletion; LC, liposomal clodronate; Ly6G; MAb, monoclonal antibody; Macrophages; Neutrophils