title: A Critical Review on Clinical Application of Separation Techniques for Selective Recognition of Uracil and 5-Fluorouracil.
abstract: The most important objectives that are frequently found in bio-analytical chemistry involve applying tools to relevant medical/biological problems and refining these applications. Developing a reliable sample preparation step, for the medical and biological fields is another primary objective in analytical chemistry, in order to extract and isolate the analytes of interest from complex biological matrices. Since, main inborn errors of metabolism (IEM) diagnosable through uracil analysis and the therapeutic monitoring of toxic 5-fluoruracil (an important anti-cancerous drug) in dihydropyrimidine dehydrogenase deficient patients, require an ultra-sensitive, reproducible, selective, and accurate analytical techniques for their measurements. Therefore, keeping in view, the diagnostic value of uracil and 5-fluoruracil measurements, this article refines several analytical techniques involved in selective recognition and quantification of uracil and 5-fluoruracil from biological and pharmaceutical samples. The prospective study revealed that implementation of molecularly imprinted polymer as a solid-phase material for sample preparation and preconcentration of uracil and 5-fluoruracil had proven to be effective as it could obviates problems related to tedious separation techniques, owing to protein binding and drastic interferences, from the complex matrices in real samples such as blood plasma, serum samples.
authors: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005 Uttar Pradesh India.
authors: Khushaboo
authors: K
authors: Pandey
authors: Tilkamanjhi Bhagalpur University, Bhagalpur, 812007 Bihar India.
authors: Rama Shankar
authors: RS
authors: Dubey
authors: Analytical Division, Chemistry Department, Faculty of Science, Banaras Hindu University, Varanasi, 221005 Uttar Pradesh India.
authors: Bhim Bali
authors: BB
authors: Prasad
keywords: 5-Fluorouracil; Bio-analytical applications; Dihydropyrimidine dehydrogenase (DPD); Separation techniques; Therapeutic drug monitoring; Uracil