title: New antibacterial isocoumarin glycosides from a wetland soil derived fungal strain Metarhizium anisopliae.
abstract: Eight new isocoumarin glycosides (1-8) were obtained from the solid culture of the wetland soil-derived fungus Metarhizium anisopliae (No. DTH12-10). Their chemical structures were elucidated by analyses of HR ESI-TOF MS, (1)H, (13)C NMR, (1)H-(1)H COSY, HSQC, and HMBC spectra. The absolute configurations were determined by single crystal X-ray diffraction, circular dichroism (CD) spectrum, and chemical derivatization methods. In addition, inhibition of the biofilm formation and the secretion of virulence factor of the new isocoumarin glycosides against Pseudomonas aeruginosa strain PAOA (clinical isolates) were evaluated. The result revealed that compound 1 showed antibacterial activity comparable with (Z)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BF).
authors: College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
authors: Jie-Feng
authors: JF
authors: Tian
authors: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China.
authors: Peng-Ju
authors: PJ
authors: Li
authors: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China.
authors: Xiao-Xia
authors: XX
authors: Li
authors: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China.
authors: Ping-Hua
authors: PH
authors: Sun
authors: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China.
authors: Hao
authors: H
authors: Gao
authors: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors: Xing-Zhong
authors: XZ
authors: Liu
authors: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China; Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Peng
authors: P
authors: Huang
authors: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China. Electronic address: gztangjinshan@126.com.
authors: Jin-Shan
authors: JS
authors: Tang
authors: College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, PR China; Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China. Electronic address: tyaoxs@jnu.edu.cn.
authors: Xin-Sheng
authors: XS
authors: Yao
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D049934:Isocoumarins / Q000737:chemistry / Q000494:pharmacology*; D052981:Metarhizium / Q000737:chemistry*; D015394:Molecular Structure; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013329:Structure-Activity Relationship; D053833:Wetlands
chemical_list: D000900:Anti-Bacterial Agents; D006027:Glycosides; D049934:Isocoumarins
keywords: 3,4-Dihydroisocoumarin glycoside; Antibacterial activity; Biofilm inhibitory activity; Chemical constituents; Metarhizium anisopliae; Virulence factor secretion