title: A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene.
abstract: Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb) carries phage-related genes and function-unknown genes. Notably, pHS87b encodes an integrase and has an adjacent tRNAThr-associated attachment site. A corresponding integrated form of pHS87b at the tRNAThr locus was identified on the chromosome of P. aeruginosa, showing that pHS87b is able to site-specifically integrate into the 3'-end of the tRNAThr gene. The pHS87a plasmid (26.8 kb) displays a plastic structure containing a putative replication module, stability factors and a variable region. The RepA of pHS87a shows significant similarity to the replication proteins of pPT23A-family plasmids. pHS87a carries a transposon Tn6049, a truncated insertion sequence ΔIS1071 and a Tn402-like class 1 integron which contains an aacA4 cassette that may confer aminoglycoside resistance. Thus, pHS87b is a site-specific integrative plasmid whereas pHS87a is a plastic antibiotic resistance plasmid. The two native plasmids may promote the fitness and evolution of P. aeruginosa.
authors: State Key Laboratory for Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai, China.
authors: Dexi
authors: D
authors: Bi
authors: State Key Laboratory for Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai, China.
authors: Yingzhou
authors: Y
authors: Xie
authors: State Key Laboratory for Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai, China.
authors: Cui
authors: C
authors: Tai
authors: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
authors: Xiaofei
authors: X
authors: Jiang
authors: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, China.
authors: Jie
authors: J
authors: Zhang
authors: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.
authors: Ewan M
authors: EM
authors: Harrison
authors: Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, China.
authors: Shiru
authors: S
authors: Jia
authors: State Key Laboratory for Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai, China.
authors: Zixin
authors: Z
authors: Deng
authors: Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom.
authors: Kumar
authors: K
authors: Rajakumar
authors: State Key Laboratory for Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai, China.
authors: Hong-Yu
authors: HY
authors: Ou
mesh_terms: D000617:Aminoglycosides; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D019143:Evolution, Molecular; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012363:RNA, Transfer, Thr / Q000235:genetics; D018741:Replication Origin / Q000235:genetics
chemical_list: D000617:Aminoglycosides; D004251:DNA Transposable Elements; D012363:RNA, Transfer, Thr