title: The importance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for correct identification of Clostridium difficile isolated from chromID C. difficile chromogenic agar.
abstract: The clinical workflow of using chromogenic agar and matrix-assisted laser desorption ionization time-of-fight mass spectrometry (MALDI-TOF MS) for Clostridium difficile identification was evaluated. The addition of MALDI-TOF MS identification after the chromID C. difficile chromogenic agar culture could significantly improve the diagnostic accuracy of C. difficile.
authors: Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
authors: Jonathan H K
authors: JHK
authors: Chen
authors: Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China; Infection Control Team, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
authors: Vincent C C
authors: VCC
authors: Cheng
authors: Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
authors: Oi-Ying
authors: OY
authors: Wong
authors: Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
authors: Sally C Y
authors: SCY
authors: Wong
authors: Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
authors: Simon Y C
authors: SYC
authors: So
authors: Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China; Infection Control Team, Queen Mary Hospital, Hong Kong Special Administrative Region, China.
authors: Wing-Cheong
authors: WC
authors: Yam
authors: Department of Microbiology, Queen Mary Hospital, Hong Kong Special Administrative Region, China; Carol Yu Centre for Infection, University of Hong Kong, Hong Kong Special Administrative Region, China. Electronic address: kyyuen@hkucc.hku.hk.
authors: Kwok-Yung
authors: KY
authors: Yuen
mesh_terms: D000362:Agar; D001431:Bacteriological Techniques / Q000379:methods*; D002863:Chromogenic Compounds / Q000737:chemistry*; D016360:Clostridioides difficile / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis / Q000382:microbiology; D003116:Color; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar
keywords: Clostridium difficile; chromogenic agar; matrix-assisted laser desorption ionization–time of flight