title: Potential of Synechocystis PCC 6803 as a novel cyanobacterial chassis for heterologous expression of enzymes in the trans-resveratrol biosynthetic pathway.
abstract: Selected model strains of phototrophic cyanobacteria have been genetically engineered for heterologous expression of numerous enzymes. In the present study, we initially explored the heterologous expression of enzymes involved in trans-resveratrol production, namely, the production of tyrosine ammonia-lyase, coumaroyl CoA-ligase, and stilbene synthase, in the unicellular cyanobacterium Synechocystis PCC 6803. Under the promoters Ptrc1Ocore and Ptrc1O, the respective genes were transcribed and translated into the corresponding soluble proteins at concentrations of 16-34 μg L(-1). The expression levels of these enzymes did not affect the growth rate of the cyanobacterial cells. Interestingly, coumaroyl CoA-ligase expression slightly increased the chlorophyll a content of the cells. Overall, our results suggest that the complete pathway of trans-resveratrol production can be engineered in Synechocystis PCC 6803.
authors: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: supaluk.tan@student.chula.ac.th.
authors: Supaluk
authors: S
authors: Tantong
authors: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: aran.i@chula.ac.th.
authors: Aran
authors: A
authors: Incharoensakdi
authors: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Omics Sciences and Bioinformatics Center, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: supaart.s@chula.ac.th.
authors: Supaart
authors: S
authors: Sirikantaramas
authors: Microbial Chemistry, Science for Life Laboratory, Department of Chemistry - Ångström, Uppsala University, Box 523, SE-75120 Uppsala, Sweden. Electronic address: Peter.Lindblad@kemi.uu.se.
authors: Peter
authors: P
authors: Lindblad
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics; D000642:Ammonia-Lyases / Q000096:biosynthesis / Q000235:genetics; D053898:Biosynthetic Pathways; D015870:Gene Expression; D011401:Promoter Regions, Genetic; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D046939:Synechocystis / Q000235:genetics*
chemical_list: D013267:Stilbenes; D000217:Acyltransferases; C055200:resveratrol synthase; C022674:stilbene synthase; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; D000077185:Resveratrol
keywords: Cyanobacteria; Heterologous expression; Synechocystis PCC 6803; Trans-resveratrol