title: Production of Omega-3 Fatty Acid Ethyl Esters from Menhaden Oil Using Proteus vulgaris Lipase-Mediated One-Step Transesterification and Urea Complexation.
abstract: An organic solvent-stable lipase from Proteus vulgaris K80 was used to produce the omega-3 polyunsaturated fatty acid ethyl esters (ω-3 PUFA EEs). First, the lyophilized recombinant lipase K80 (LyoK80) was used to perform the transesterification reaction of menhaden oil and ethanol. LyoK80 produced the ω-3 PUFA EEs with a conversion yield of 82 % in the presence of 20 % water content via a three-step ethanol-feeding process; however, in a non-aqueous condition, LyoK80 produced only a slight amount of the ω-3 PUFA EEs. To enhance its reaction properties, the lipase K80 was immobilized on a hydrophobic bead to derive ImmK80; the biochemical properties and substrate specificity of ImmK80 are similar to those of LyoK80. ImmK80 was then used to produce ω-3 PUFA EEs in accordance with the same transesterification reaction. Unlike LyoK80, ImmK80 achieved a high ω-3 PUFA EE conversion yield of 86 % under a non-aqueous system via a one-step ethanol-feeding reaction. The ω-3 PUFA EEs were purified up to 92 % using a urea complexation method.
authors: Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, 420-743, Korea.
authors: Soo-Jin
authors: SJ
authors: Kim
authors: Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, 420-743, Korea. hkkim@catholic.ac.kr.
authors: Hyung Kwoun
authors: HK
authors: Kim
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D004952:Esters / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis* / Q000737:chemistry; D005395:Fish Oils / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D011514:Proteus vulgaris / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D014508:Urea / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D004952:Esters; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D012997:Solvents; C033266:Menhaden oil; D000431:Ethanol; D014508:Urea; D008049:Lipase
keywords: Immobilization; Lipase; Omega-3 fatty acid ethyl esters; Transesterification; Urea complexation