title: Frequencies of EGFR single nucleotide polymorphisms in non-small cell lung cancer patients and healthy individuals in the Republic of Serbia: a preliminary study.
abstract: The purpose of this study was to determine the frequencies of EGFR -216G>T, -191C>A, and 181946C>T in Serbian non-small cell lung cancer (NSCLC) patients, as well as to compare it with healthy individuals, in order to assess their potential importance for lung cancer in Serbia. The study involved 56 NSCLC patients and 53 unrelated healthy volunteers, and genotyping was performed on DNA samples obtained from formalin-fixed paraffin-embedded lung tumor tissue and blood, respectively. This was the first time to show genotype frequencies of those single nucleotide polymorphisms for this study group from the territory of the Republic of Serbia. There was very strong evidence of association between age and death due to lung cancer (Pearson chi-square = 43.439, df = 2, p < 0,001), as well as between ever smoking and death due to lung cancer (Pearson chi-square = 31.727, df = 1, p < 0.001). When dominant genetic model (GG vs. GT+TT) was used for -216G>T, we have found significant association (p = 0.012) between -216GG genotype and NSCLC patients within smokers' subgroup. So, carriers of -216GG genotype had higher risk (OR = 4.33, 95 % CI = 1.324-14.179) than noncarriers (GT and TT) for developing non-small cell lung cancer in our patients.
authors: Institute of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000, Kragujevac, Serbia.
authors: Jasmina
authors: J
authors: Obradović
authors: Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia.
authors: Natasa
authors: N
authors: Djordjević
authors: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010, Belgrade, Serbia.
authors: Natasa
authors: N
authors: Tošic
authors: Institute for Oncology of Vojvodina, Put Doktora Goldmana 4, 21204, Sremska Kamenica, Serbia.
authors: Jasminka
authors: J
authors: Mrdjanović
authors: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010, Belgrade, Serbia.
authors: Biljana
authors: B
authors: Stanković
authors: Institute for Pulmonary Diseases of Vojvodina, Clinic for Pulmonary Oncology, Faculty of Medicine, University of Novi Sad, Put Doktora Goldmana 4, 21204, Sremska Kamenica, Serbia.
authors: Jelena
authors: J
authors: Stanić
authors: Institute for Pulmonary Diseases of Vojvodina, Clinic for Pulmonary Oncology, Faculty of Medicine, University of Novi Sad, Put Doktora Goldmana 4, 21204, Sremska Kamenica, Serbia.
authors: Bojan
authors: B
authors: Zarić
authors: Institute for Pulmonary Diseases of Vojvodina, Clinic for Pulmonary Oncology, Faculty of Medicine, University of Novi Sad, Put Doktora Goldmana 4, 21204, Sremska Kamenica, Serbia.
authors: Branislav
authors: B
authors: Perin
authors: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, 11010, Belgrade, Serbia.
authors: Sonja
authors: S
authors: Pavlović
authors: Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia. vdvd@lycos.com.
authors: Vladimir
authors: V
authors: Jurišić
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D052138:Genes, Neoplasm; D018773:Genes, erbB-1; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D055771:Serbia / Q000453:epidemiology; D012907:Smoking / Q000453:epidemiology / Q000235:genetics; D055815:Young Adult
chemical_list: D004273:DNA, Neoplasm
keywords: Epidermal growth factor receptor; Non-small cell lung cancer; Single nucleotide polymorphism