title: HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries.
abstract: We present and evaluate an approach for coupling liquid chromatography in glass chips with mass spectrometry via fully integrated electrospray emitters. We developed an instrumental platform which allows a robust and reproducible operation of high performance chip chromatography coupled to mass spectrometry. A comparison of differently shaped emitters, from flat over edged to pulled geometries, revealed that all types performed equally well for typical nano-HPLC flow rates. At very low flow rates below 50 nL·min(-1) very sharp, pulled nanospray emitters turned out to be mandatory for the generation of a stable electrospray.
authors: Institute of Analytical Chemistry, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors: Carsten
authors: C
authors: Lotter
authors: Institute of Analytical Chemistry, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors: Josef J
authors: JJ
authors: Heiland
authors: Institute of Analytical Chemistry, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors: Sebastian
authors: S
authors: Thurmann
authors: Institute of Analytical Chemistry, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors: Laura
authors: L
authors: Mauritz
authors: Institute of Analytical Chemistry, University of Leipzig , Linnéstraße 3, 04103 Leipzig, Germany.
authors: Detlev
authors: D
authors: Belder