title: Effects of different periods of paradoxical sleep deprivation and sleep recovery on lipid and glucose metabolism and appetite hormones in rats.
abstract: Sleep has a fundamental role in the regulation of energy balance, and it is an essential and natural process whose precise impacts on health and disease have not yet been fully elucidated. The aim of this study was to assess the consequences of different periods of paradoxical sleep deprivation (PSD) and recovery from PSD on lipid profile, oral glucose tolerance test (OGTT) results, and changes in insulin, corticosterone, ghrelin, and leptin concentrations. Three-month-old male Wistar rats weighing 250-350 g were submitted to 24, 96, or 192 h of PSD or 192 h of PSD with 480 h of recovery. The PSD was induced by the multiple platforms method. Subsequently, the animals were submitted to an OGTT. One day later, the animals were killed and the levels of triglycerides, total cholesterol, lipoproteins (low-density lipoprotein, very-low-density lipoprotein, and high-density lipoprotein), insulin, ghrelin, leptin, and corticosterone in plasma were quantified. There was a progressive decrease in body weight with increasing duration of PSD. The PSD induced basal hypoglycemia over all time periods evaluated. Evaluation of areas under the curve revealed progressive hypoglycemia only after 96 and 192 h of PSD. There was an increase in corticosterone levels after 192 h of PSD. We conclude that PSD induces alterations in metabolism that are reversed after a recovery period of 20 days.
authors: a Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, Mexico.
authors: Malinalli
authors: M
authors: Brianza-Padilla
authors: b Departamento de Biología de la Reproducción, Área de Biología Conductual y Reproductiva, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, CP 09340, Mexico.
authors: Herlinda
authors: H
authors: Bonilla-Jaime
authors: c Departamento de Ciencias de la Salud, Área de Investigación Médica, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, Mexico.
authors: Julio César
authors: JC
authors: Almanza-Pérez
authors: a Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, Mexico.
authors: Ana Laura
authors: AL
authors: López-López
authors: d Departamento de Inmunología, Instituto Nacional de Cardiologia (Ignacio Chávez), Juan Badiano No. 1, Col. Sección XVI, Del. Tlalpan, CP 14080, Mexico.
authors: Fausto
authors: F
authors: Sánchez-Muñoz
authors: e Colegio de Ciencias y Humanidades, Universidad Autónoma de la Ciudad de México-San Lorenzo Tezonco, Av. Prolongación San Isidro No. 151, Col. San Lorenzo Tezonco, Del. Iztapalapa, CP 09790, Mexico.
authors: Gonzalo
authors: G
authors: Vázquez-Palacios
mesh_terms: D000818:Animals; D001069:Appetite Regulation; D015415:Biomarkers; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight; D003345:Corticosterone / Q000097:blood; D004195:Disease Models, Animal; D004435:Eating; D004734:Energy Metabolism; D054439:Ghrelin / Q000097:blood; D005951:Glucose Tolerance Test; D006728:Hormones / Q000097:blood*; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood*; D008297:Male; D017208:Rats, Wistar; D020127:Recovery of Function; D012890:Sleep; D012892:Sleep Deprivation / Q000097:blood* / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D054439:Ghrelin; D006728:Hormones; D007328:Insulin; D020738:Leptin; D008055:Lipids; D003345:Corticosterone
keywords: appetite regulation; glucose metabolism; métabolisme du glucose; paradoxical sleep deprivation; privation de sommeil; privation de sommeil paradoxal; régulation de l’appétit; sleep deprivation