title: Distinct effects of Lactobacillus plantarum KL30B and Escherichia coli 3A1 on the induction and development of acute and chronic inflammation.
abstract: OBJECTIVE
  Enteric bacteria are involved in the pathogenesis of ulcerative colitis. In experimental colitis, a breakdown of the intestinal epithelial barrier results in inflow of various gut bacteria, induction of acute inflammation and finally, progression to chronic colitis.
  
  
  METHODS
  In the present study we compared pro-inflammatory properties of two bacterial strains isolated from human microbiome, Escherichia coli 3A1 and Lactobacillus plantarum KL30B. The study was performed using two experimental models of acute inflammation: peritonitis in mice and trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats.
  
  
  RESULTS
  Both bacterial strains induced massive neutrophil infiltration upon injection into sterile peritoneal cavity. However, peritoneal exudate cells stimulated in vitro with E. coli 3A1, produced far more nitric oxide, than those stimulated with L. plantarum KL30B. Interestingly, distinct effect on the development of TNBS-induced colitis was observed after oral administration of the tested bacteria. Lactobacillus plantarum KL30B evoked strong acute colitis. On the contrary, the administration of E. coli 3A1 resulted in a progression of colitis to chronicity.
  
  
  CONCLUSIONS
  Our results show that distinct effects of bacterial administration on the development of ongoing inflammation is strain specific and depends on the final effect of cross-talk between bacteria and cells of the innate immune system.
authors: Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland; University Center for Veterinary Medicine, Jagiellonian University and University of Agriculture, Krakow, Poland.
authors: Magdalena
authors: M
authors: Strus
authors: Chair of Pathomorphology, Jagiellonian University Medical College, Krakow, Poland.
authors: Krzysztof
authors: K
authors: Okoń
authors: Chair of Immunology, Jagiellonian University Medical College, Krakow, Poland.
authors: Bernadeta
authors: B
authors: Nowak
authors: Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland.
authors: Magdalena
authors: M
authors: Pilarczyk-Zurek
authors: Chair of Microbiology, Jagiellonian University Medical College, Krakow, Poland.
authors: Piotr
authors: P
authors: Heczko
authors: Chair of Immunology, Jagiellonian University Medical College, Krakow, Poland.
authors: Anna
authors: A
authors: Gawda
authors: Chair of Immunology, Jagiellonian University Medical College, Krakow, Poland.
authors: Marta
authors: M
authors: Ciszek-Lenda
authors: Department of Pathophysiology, Jagiellonian University Medical College, Krakow, Poland.
authors: Beata
authors: B
authors: Skowron
authors: Department of Pathophysiology, Jagiellonian University Medical College, Krakow, Poland.
authors: Agnieszka
authors: A
authors: Baranowska
authors: University Center for Veterinary Medicine, Jagiellonian University and University of Agriculture, Krakow, Poland; Chair of Immunology, Jagiellonian University Medical College, Krakow, Poland.
authors: Janusz
authors: J
authors: Marcinkiewicz
keywords: Escherichia coli; Lactobacillus plantarum; TNBS-induced colitis; inflammation; neutrophils