title: Haematospirillum jordaniae gen. nov., sp. nov., isolated from human blood samples.
abstract: A Gram-negative, aerobic, motile, spiral-shaped bacterium, strain H5569(T), was isolated from a human blood sample. Phenotypic and molecular characteristics of the isolate were investigated. Optimal growth was found to occur at 35 °C under aerobic conditions on Heart Infusion Agar supplemented with 5 % rabbit blood. The major fatty acids present in the cells were identified as C16:0, C16:1ω7c and C18:1ω7c. The predominant respiratory quinone was found to be ubiquinone-Q10. The G+C content of genomic DNA for strain H5569(T) was found to be 49.9 %. Based on 16S rRNA gene sequence analysis results, 13 additional isolates were also analysed in this study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism, represented by strain H5569(T), forms a distinct lineage within the family Rhodospirillaceae, closely related to two Novispirillum itersonii subspecies (93.9-94.1 %) and two Caenispirillum sp. (91.2-91.6 %). Based on these results, the isolate H5569(T) is concluded to represent a new genus and species for which the name Haematospirillum jordaniae gen. nov., sp. nov. is proposed. The type strain is H5569(T) (=DSM(T) 28903 = CCUG 66838(T)).
authors: Special Bacteriology Reference Laboratory, Bacterial Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, USA. Bhumrighouse@cdc.gov.
authors: B W
authors: BW
authors: Humrighouse
authors: Special Bacteriology Reference Laboratory, Bacterial Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, USA.
authors: B D
authors: BD
authors: Emery
authors: Special Bacteriology Reference Laboratory, Bacterial Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, USA.
authors: A J
authors: AJ
authors: Kelly
authors: Infectious Diseases Pathology Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, USA.
authors: M G
authors: MG
authors: Metcalfe
authors: Department of Public Health, Clinical and Health Sciences, College of Science, Engineering and Health, University of West Florida, Pensacola, USA.
authors: J
authors: J
authors: Mbizo
authors: Special Bacteriology Reference Laboratory, Bacterial Special Pathogens Branch, Division of High Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, USA.
authors: J R
authors: JR
authors: McQuiston
mesh_terms: D000328:Adult; D000368:Aged; D001482:Base Composition; D001483:Base Sequence; D001769:Blood / Q000382:microbiology*; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000097:blood* / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Bacteria; Blood; Clinical; Gram-negative; Haematospirillum jordaniae; Pathogen