title: Adsorption of T4 bacteriophages on planar indium tin oxide surface via controlled surface tailoring.
abstract: The work investigates the influence of surface physicochemical properties of planar indium tin oxide (ITO) as a model substrate on T4 bacteriophage adsorption. A comparative T4 bacteriophage adsorption study shows a significant difference in bacteriophage adsorption observed on chemically modified planar ITO when compared to similarly modified particulate ITO, which infers that trends observed in virus-particle interaction studies are not necessarily transferrable to predict virus-planar surface adsorption behaviour. We also found that ITO surfaces modified with methyl groups, (resulting in increased surface roughness and hydrophobicity) remained capable of adsorbing T4 bacteriophage. The adsorption of T4 onto bare, amine and carboxylic functionalised planar ITO suggests the presence of a unique binding behaviour involving specific functional groups on planar ITO surface beyond the non-specific electrostatic interactions that dominate phage to particle interactions. The paper demonstrates the significance of physicochemical properties of surfaces on bacteriophage-surface interactions.
authors: School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia.
authors: Ayu Ekajayanthi
authors: AE
authors: Liana
authors: School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia.
authors: Ed Win
authors: EW
authors: Chia
authors: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: c.marquis@unsw.edu.au.
authors: Christopher P
authors: CP
authors: Marquis
authors: School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia; ithree Institute, University of Technology Sydney, Sydney, NSW 2007, Australia. Electronic address: cindy.gunawan@uts.edu.au.
authors: Cindy
authors: C
authors: Gunawan
authors: School of Chemistry and Australian Centre for NanoMedicine, The University of New South Wales, Sydney, NSW 2052, Australia.
authors: J Justin
authors: JJ
authors: Gooding
authors: School of Chemical Engineering, The University of New South Wales, Sydney, NSW 2052, Australia.
authors: Rose
authors: R
authors: Amal
mesh_terms: D000327:Adsorption; D017122:Bacteriophage T4 / Q000737:chemistry* / Q000302:isolation & purification; D002627:Chemistry, Physical; D010316:Particle Size; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D017971:Tin Compounds; C109984:indium tin oxide
keywords: Adsorption; Bacteriophage; Bio-nano interactions; Surface modifications; Virus-surface interactions