title: Distant effects of unilateral renal ischemia/reperfusion on contralateral kidney but not lung in rats: the roles of ROS and iNOS.
abstract: Acute kidney injury is usually associated with distant organ dysfunction. The roles of inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) in this phenomenon were investigated following 2 h unilateral renal ischemia and 24 h reperfusion. There were 3 groups of rats subjected to either unilateral ischemia/reperfusion (UIR group), unilateral nephrectomy (UNX group), or sham operation. Two further groups were given α-tocopherol and aminoguanidine with UIR (treated-UIR group) and UNX (treated-UNX group). Plasma nitrite/nitrate and malondialdehyde were elevated only in the UIR group. Creatinine clearance and blood flow increased in non-ischemic kidney of the UIR, but not to the same extent as remnant kidney of the UNX group, while they had equal compensatory rises in absolute Na(+) and K(+) excretion and urine flow. Non-ischemic kidney of the treated-UIR group, but not remnant kidney of the treated-UNX group, showed more elevation in blood flow, whereas both kidneys had reductions in absolute Na(+) excretion and urine flow. Respiratory functional variable were not different between all groups. Therefore, 2 h unilateral renal ischemia and 24 h reperfusion did not affect lung but had distant effects on contralateral kidney partly mediated by ROS and NO-derived from iNOS to dampen compensatory increases in renal hemodynamics and to decrease tubular reabsorption.
authors: Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.|Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.
authors: Hossein
authors: H
authors: Fatemikia
authors: Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.|Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.
authors: Farzaneh
authors: F
authors: Ketabchi
authors: Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.|Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.
authors: Zynab
authors: Z
authors: Karimi
authors: Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.|Department of Physiology, The Medical School, Shiraz University of Medical Sciences, Shiraz 71365-1689, Iran.
authors: Seyed Mostafa Shid
authors: SM
authors: Moosavi
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D006146:Guanidines / Q000627:therapeutic use; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009392:Nephrectomy / Q000009:adverse effects; D009566:Nitrates / Q000097:blood; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism*; D065608:Renal Reabsorption / Q000187:drug effects; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D012119:Respiration / Q000187:drug effects; D014555:Urination Disorders / Q000209:etiology / Q000517:prevention & control; D024502:alpha-Tocopherol / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D006146:Guanidines; D009566:Nitrates; D009573:Nitrites; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D024502:alpha-Tocopherol; C004479:pimagedine
keywords: acute kidney injury; acute lung injury; alpha-tocopherol; alpha-tocophérol; aminoguanidine; dommage pulmonaire aigu; dommage rénal aigu; espèces réactives d’oxygène; inducible nitric oxide synthase; ischemia/reperfusion; ischémie rénale unilatérale; ischémie/reperfusion; néphrectomie unilatérale; reactive oxygen species; synthase d’oxyde nitrique inductible; unilateral nephrectomy; unilateral renal ischemia