title: Protein import complexes in the mitochondrial outer membrane of Amoebozoa representatives.
abstract: BACKGROUND
  An ancestral trait of eukaryotic cells is the presence of mitochondria as an essential element for function and survival. Proper functioning of mitochondria depends on the import of nearly all proteins that is performed by complexes located in both mitochondrial membranes. The complexes have been proposed to contain subunits formed by proteins common to all eukaryotes and additional subunits regarded as lineage specific. Since Amoebozoa is poorly sampled for the complexes we investigated the outer membrane complexes, namely TOM, TOB/SAM and ERMES complexes, using available genome and transcriptome sequences, including transcriptomes assembled by us.
  
  
  RESULTS
  The results indicate differences in the organization of the Amoebozoa TOM, TOB/SAM and ERMES complexes, with the TOM complex appearing to be the most diverse. This is reflected by differences in the number of involved subunits and in similarities to the cognate proteins of representatives from different supergroups of eukaryotes.
  
  
  CONCLUSIONS
  The obtained results clearly demonstrate structural variability/diversity of these complexes in the Amoebozoa lineage and the reduction of their complexity as compared with the same complexes of model organisms.
authors: Institute of Bioinformatics, Faculty of Medicine, University of Muenster, Niels Stensen Strasse 14, 48149, Muenster, Germany. dorotabuczek81@gmail.com.|Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland. dorotabuczek81@gmail.com.
authors: Dorota
authors: D
authors: Buczek
authors: Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland. woytek@amu.edu.pl.
authors: Małgorzata
authors: M
authors: Wojtkowska
authors: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan. ysuzuki@k.u-tokyo.ac.jp.
authors: Yutaka
authors: Y
authors: Suzuki
authors: Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Kobe, Hyogo, 678-1297, Japan. sonobe@sci.u-hyogo.ac.jp.
authors: Seiji
authors: S
authors: Sonobe
authors: Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Kobe, Hyogo, 678-1297, Japan. amoeba_gami@yahoo.co.jp.
authors: Yukinori
authors: Y
authors: Nishigami
authors: Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland.
authors: Monika
authors: M
authors: Antoniewicz
authors: Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland. kmita@amu.edu.pl.
authors: Hanna
authors: H
authors: Kmita
authors: Institute of Bioinformatics, Faculty of Medicine, University of Muenster, Niels Stensen Strasse 14, 48149, Muenster, Germany. wojmak@uni-muenster.de.
authors: Wojciech
authors: W
authors: Makałowski
mesh_terms: D000595:Amino Acid Sequence; D056894:Amoebozoa / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D017124:Conserved Sequence; D005091:Exons; D014644:Genetic Variation; D018503:Genome, Protozoan; D007438:Introns; D051336:Mitochondrial Membranes / Q000378:metabolism*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D046912:Multiprotein Complexes / Q000378:metabolism*; D010802:Phylogeny; D021122:Protein Subunits; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D002352:Carrier Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; D015800:Protozoan Proteins