title: Analysis of the expression and localization of tight junction transmembrane proteins, claudin-1, -4, -7, occludin and JAM-A, in human cervical adenocarcinoma.
abstract: OBJECTIVE
  Tight junction proteins have recently been reported to be useful for distinguishing between neoplastic and non-neoplastic tissues. In this study, we evaluated the expression and localization of tight junction transmembrane proteins in human cervical adenocarcinoma and adenocarcinoma in situ (AIS), and we determined whether their expression patterns could distinguish cervical adenocarcinoma from non-neoplastic cervical glands.
  
  
  METHODS
  Fifty-five patients with cervical adenocarcinoma or AIS were included in this study. Surgical specimens were immunohistochemically stained for claudin (CLDN) -1, -4, -7, occludin, and JAM-A.
  
  
  RESULTS
  Significantly higher expression levels of CLDNs and JAM-A were found in cervical AIS and adenocarcinoma than in non-neoplastic glands. In cervical AIS and adenocarcinoma, localization of CLDN1 and JAM-A was extended throughout the whole cell membranes, whereas they were predominantly expressed at the most apical cell-cell junction in non-neoplastic glands. ROC curve analysis revealed that immunoreactivities of CLDN-1 or JAM-A successfully distinguished neoplasms from non-neoplastic cervical glands with high specificity (CLDN-1, 79.1%; JAM-A, 79.1%) and high sensitivity (CLDN-1, 84.1%; JAM-A, 95.5%).
  
  
  CONCLUSIONS
  As expected, there were immunohistochemical differences between cervical adenocarcinoma and non-neoplastic cervical glands by using antibodies against tight junction transmembrane proteins. These results suggest that CLDN-1 and JAM-A are potential biomarkers for cervical adenocarcinoma.
authors: Department of Obstetrics and Gynecology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Taishi
authors: T
authors: Akimoto
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan. atakasawa@sapmed.ac.jp.
authors: Akira
authors: A
authors: Takasawa
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Masaki
authors: M
authors: Murata
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Yui
authors: Y
authors: Kojima
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Kumi
authors: K
authors: Takasawa
authors: Division of Advanced Medicine Promotion, The Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors: Masanori
authors: M
authors: Nojima
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Tomoyuki
authors: T
authors: Aoyama
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Yutaro
authors: Y
authors: Hiratsuka
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Yusuke
authors: Y
authors: Ono
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Satoshi
authors: S
authors: Tanaka
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Makoto
authors: M
authors: Osanai
authors: Department of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Tadashi
authors: T
authors: Hasegawa
authors: Department of Obstetrics and Gynecology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Tsuyoshi
authors: T
authors: Saito
authors: Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Norimasa
authors: N
authors: Sawada
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis*; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis; D062445:Claudin-1 / Q000032:analysis / Q000096:biosynthesis; D062506:Claudin-4 / Q000032:analysis / Q000096:biosynthesis; D057167:Claudins / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D062793:Occludin / Q000032:analysis / Q000096:biosynthesis; D012372:ROC Curve; D011956:Receptors, Cell Surface / Q000032:analysis / Q000096:biosynthesis; D012680:Sensitivity and Specificity; D062725:Tight Junction Proteins / Q000032:analysis / Q000096:biosynthesis*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis*; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis*
chemical_list: D014408:Biomarkers, Tumor; C473255:CLDN7 protein, human; D015815:Cell Adhesion Molecules; D062445:Claudin-1; D062506:Claudin-4; D057167:Claudins; C407243:F11R protein, human; C568985:OCLN protein, human; D062793:Occludin; D011956:Receptors, Cell Surface; D062725:Tight Junction Proteins