title: Improvement of Infusion Process in Cell Transplantation: Effect of Shear Stress on Hepatocyte Viability Under Horizontal and Vertical Syringe Orientation.
abstract: Improving cell viability and function are important for enhancing the clinical results of cell transplantation. The relationship between cell viability and shear stress remains unexplained, and sedimentation effects during the infusion process are important to the hepatocyte transplantation process. In the present study, the relationship between cell viability and shear stress in the presence of sedimentation effect was investigated using a microchannel simulating the cell transplantation process under several shear stress conditions. Horizontal and vertical syringe orientations were employed to investigate the sedimentation effect. The vertical syringe orientation resulted in lower viability loss than the horizontal orientation. In summary, removing a sedimentation effect is important to improving cell viability by preventing high shear stress.
authors: Department of Mechanical Engineering, Graduate School of Science and Engineering, Tokyo Metropolitan University , Hachioji, Tokyo , Japan.
authors: Sandi
authors: S
authors: Sufiandi
authors: Department of Mechanical Engineering, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo, Japan; †Clinical Research Center, National Center for Child Health and Development, Setagaya-ku, Tokyo, Japan.
authors: Hiromichi
authors: H
authors: Obara
authors: † Clinical Research Center, National Center for Child Health and Development , Setagaya-ku, Tokyo , Japan.
authors: Shin
authors: S
authors: Enosawa
authors: † Clinical Research Center, National Center for Child Health and Development , Setagaya-ku, Tokyo , Japan.
authors: Huai-Che
authors: HC
authors: Hsu
authors: Department of Mechanical Engineering, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo, Japan; †Clinical Research Center, National Center for Child Health and Development, Setagaya-ku, Tokyo, Japan.
authors: Naoto
authors: N
authors: Matsuno
authors: Department of Mechanical Engineering, Graduate School of Science and Engineering, Tokyo Metropolitan University , Hachioji, Tokyo , Japan.
authors: Hiroshi
authors: H
authors: Mizunuma
keywords: Hepatocytes; Rectangular microchannel; Shear stress; Syringe orientation; Viability loss