title: Identification of proteins regulated by curcumin in cerebral ischemia.
abstract: BACKGROUND
  Curcumin is known to have a neuroprotective effect against cerebral ischemia. The objective of this study was to identify various proteins that are differentially expressed by curcumin treatment in focal cerebral ischemia using a proteomic approach.
  
  
  METHODS
  Adult male rats were treated with vehicle or curcumin 1 h after middle cerebral artery occlusion. Brain tissues were collected 24 h after the onset of middle cerebral artery occlusion, and cerebral cortices proteins were identified by two-dimensional gel electrophoresis and mass spectrometry.
  
  
  RESULTS
  We detected several proteins with altered expression levels between vehicle- and curcumin-treated animals. Among these proteins, ubiquitin carboxy-terminal hydrolase L1, isocitrate dehydrogenase, adenosylhomocysteinase, and eukaryotic initiation factor 4A were decreased in the vehicle-treated animal, and curcumin treatment attenuated the injury-induced decreases of these proteins. Conversely, pyridoxal phosphate phosphatase was increased in the vehicle-treated animal, and curcumin treatment prevented decreases in this protein. The identified altered proteins are associated with cellular metabolism and differentiation.
  
  
  CONCLUSIONS
  The results of this study suggest that curcumin exerts a neuroprotective effect by regulating the expression of various proteins in focal cerebral ischemia.
authors: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea; Division of Life Science and Applied Life Science, Gyeongsang National University, Jinju, South Korea.
authors: Fawad-Ali
authors: FA
authors: Shah
authors: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.
authors: Sang-Ah
authors: SA
authors: Gim
authors: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.
authors: Jin-Hee
authors: JH
authors: Sung
authors: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.
authors: Seong-Jun
authors: SJ
authors: Jeon
authors: Division of Life Science and Applied Life Science, Gyeongsang National University, Jinju, South Korea.
authors: Myeong-Ok
authors: MO
authors: Kim
authors: Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea. Electronic address: pokoh@gnu.ac.kr.
authors: Phil-Ok
authors: PO
authors: Koh
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011897:Random Allocation; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; D003474:Curcumin
keywords: Curcumin; Proteomics; Stroke