title: Development of a Two-Photon Fluorescent Probe for Imaging of Endogenous Formaldehyde in Living Tissues.
abstract: Investigation of the physiological and pathological functions of formaldehyde (FA) are largely restricted by a lack of useful FA imaging agents, in particular, those that allow detection of FA in the context of living tissues. Herein, we present the rational design, synthesis, and photophysical property studies of the first two-photon fluorescent FA probe, Na-FA. Importantly, the highly desirable attributes of the probe Na-FA (such as a very large turn-on signal (up to 900-fold), a low detection limit, and a very fast onset imparted by the unique design aspects of the probe), make it possible to monitor endogenous FA in living tissues for the first time. Furthermore, sodium bisulfite was identified as a simple and convenient inhibitor of FA within biological environments.
authors: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science, University of Jinan, Jinan, Shandong, 250022, P.R. China.
authors: Yonghe
authors: Y
authors: Tang
authors: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science, University of Jinan, Jinan, Shandong, 250022, P.R. China.
authors: Xiuqi
authors: X
authors: Kong
authors: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science, University of Jinan, Jinan, Shandong, 250022, P.R. China.
authors: An
authors: A
authors: Xu
authors: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science, University of Jinan, Jinan, Shandong, 250022, P.R. China.
authors: Baoli
authors: B
authors: Dong
authors: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Biological Science, University of Jinan, Jinan, Shandong, 250022, P.R. China. weiyinglin2013@163.com.
authors: Weiying
authors: W
authors: Lin
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D005557:Formaldehyde / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D017785:Photons
chemical_list: D005456:Fluorescent Dyes; D005557:Formaldehyde
keywords: endogenous formaldehyde; fluorescence imaging; fluorescent probes; living tissues; two-photon fluorescence