title: Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line.
abstract: Thorough examination of genetic heterogeneity of cell lines is uncommon. In order to address this issue, the present study analyzed the genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia (T-ALL) cell line. For this purpose, traditional techniques such as fluorescencein situhybridization and immunocytochemistry were used, in addition to more advanced techniques, including cell sorting, Sanger sequencing and massive parallel sequencing. The results indicated that the RPMI-8402 cell line consists of several genetically different cell subpopulations. Furthermore, massive parallel sequencing of RPMI-8402 provided insight into the evolution of T-ALL carcinogenesis, since this cell line exhibited the genetic heterogeneity typical of T-ALL. Therefore, the use of cell lines for drug testing in future studies may aid the progress of anticancer drug research.
authors: Department of Tumor Biology, Medical University of Łódź, Łódź 90-752, Poland.
authors: Ewelina
authors: E
authors: Stoczynska-Fidelus
authors: Department of Tumor Biology, Medical University of Łódź, Łódź 90-752, Poland.
authors: Sylwester
authors: S
authors: Piaskowski
authors: Department of Research and Development, Celther Polska Ltd., Łódź 93-193, Poland; Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Łódź 90-363, Poland.
authors: Roza
authors: R
authors: Pawlowska
authors: Department of Microbiology and Medical Laboratory Immunology, Medical University of Łódź, Łódź 92-213, Poland.
authors: Malgorzata
authors: M
authors: Szybka
authors: Department of Tumor Biology, Medical University of Łódź, Łódź 90-752, Poland; Department of Research and Development, Celther Polska Ltd., Łódź 93-193, Poland.
authors: Joanna
authors: J
authors: Peciak
authors: Department of Tumor Biology, Medical University of Łódź, Łódź 90-752, Poland.
authors: Krystyna
authors: K
authors: Hulas-Bigoszewska
authors: Department of Tumor Biology, Medical University of Łódź, Łódź 90-752, Poland.
authors: Marta
authors: M
authors: Winiecka-Klimek
authors: Department of Tumor Biology, Medical University of Łódź, Łódź 90-752, Poland.
authors: Piotr
authors: P
authors: Rieske
keywords: RPMI-8402; TP53; genetic heterogeneity; massive parallel sequencing; mutation; oncogenetic tree