title: Comparative analysis of microRNA in schistosomula isolated from non-permissive host and susceptible host.
abstract: The reed vole Microtus fortis is the only known mammal in which the schistosome is naturally prevented from maturing and schistosome infection does not cause significant pathogenesis. However, the mechanism behind this phenomenon remains unknown. In the present study, Solexa deep sequencing technology was used to carry out high-throughput sequencing and comparative analysis of microRNA (miRNA) between small RNA libraries isolated from 10 days oldschistosomula of M. fortis and BALB/c mice.In total, 10d schistosomula from M. fortis and BALB/c mice yielded 13.37 and 10.84 million reads, respectively, and nearly 39% and 40% of reads could be mapped to selected miRNAs in miRbase. Based on a bioinformatic analysis, we found that most of the miRNAs identified in Schistosoma japonicum were detected in our study. Further analysis revealed that 24 miRNAs were differentially expressed between the schistosomula from the two rodents, of which 21 were down-regulated and three were up-regulated in schistosomula from M. fortis. Also, six novel miRNAs were predicted and identified in this study. Target genes were mapped and filtered by correlating them with differentially expressed genes obtained from S. japonicum oligonucleotide microarray analyses performed in previous studies. miRNAs such as miR-10-3p, miR-10-5p, and miR-2b-5p may affect the growth, differentiation, and metabolism of worms via regulation of the expression of target genes such as enolase, aquaporin, TGF-beta-inducible nuclear protein, and paramyosin. Gene Ontology analysis of the predicted target genes of these six differentially expressed miRNAs revealed that some important biological pathways, such as metabolic processes,glycolysis, and catalytic activity, were involved. The results of this study highlight the function of miRNAs in the development and survival of the schistosome, and provide valuable information to increase our understanding of the regulatory function of miRNAs in schistosome development and host-parasite interactions in a differentially susceptible host environment.
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China; Minhang Animal Disease Control Center, Shanghai 201109, China. Electronic address: hanhongxiao82@aliyun.com.
authors: Hongxiao
authors: H
authors: Han
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China. Electronic address: pengjinbiao2008@aliyun.com.
authors: Jinbiao
authors: J
authors: Peng
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China. Electronic address: hongyang_7@126.com.
authors: Yang
authors: Y
authors: Hong
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China. Electronic address: fuzhiqiang@shvri.ac.cn.
authors: Zhiqiang
authors: Z
authors: Fu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China. Electronic address: luke@shvri.ac.cn.
authors: Ke
authors: K
authors: Lu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China. Electronic address: lihao@shvri.ac.cn.
authors: Hao
authors: H
authors: Li
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China. Electronic address: zcg@shvri.ac.cn.
authors: Chuangang
authors: C
authors: Zhu
authors: Minhang Animal Disease Control Center, Shanghai 201109, China. Electronic address: 441634020@qq.com.
authors: Qiuhua
authors: Q
authors: Zhao
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai 200241, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: jjlin@shvri.ac.cn.
authors: Jiaojiao
authors: J
authors: Lin
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000469:parasitology*; D020869:Gene Expression Profiling; D058507:Host Specificity; D006790:Host-Parasite Interactions; D051379:Mice / Q000469:parasitology*; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012549:Schistosoma japonicum / Q000235:genetics* / Q000378:metabolism; D012554:Schistosomiasis japonica / Q000276:immunology / Q000469:parasitology*
chemical_list: D035683:MicroRNAs
keywords: Deep sequencing; MicroRNA; Microtus fortis; Schistosoma japonicum