title: Cytotoxicity and colloidal behavior of polystyrene latex nanoparticles toward filamentous fungi in isotonic solutions.
abstract: The effects of surface physicochemical properties of functionalized polystyrene latex (PSL) nanoparticles (NPs) and model filamentous fungi Aspergillus oryzae and Aspergillus nidulans cultivated in different environment (aqueous and atmospheric environment) on the colloidal behavior and cytotoxicity were investigated in different isotonic solutions (154 mM NaCl and 292 mM sucrose). When the liquid cultivated fungal cells were exposed to positively charged PSL NPs in 154 mM NaCl solution, the NPs were taken into A. oryzae, but not A. nidulans. Atomic force microscopy revealed that the uptake of NPs was more readily through the cell wall of A. oryzae because of its relatively softer cell wall compared with A. nidulans. In contrast, the positively charged PSL NPs entirely covered the liquid cultivated fungal cell surfaces and induced cell death in 292 mM sucrose solution because of the stronger electrostatic attractive force between the cells and NPs compared with in 154 mM NaCl. When the agar cultivated fungal cells were exposed to the positively charged PSL NPs, both fungal cells did not take the NPs inside the cells. Contact angle measurement revealed that the hydrophobin on the agar cultivated cell surfaces inhibited the uptake of NPs because of its relatively more hydrophobic cell surface compared with the liquid cultivated cells.
authors: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Electronic address: nomura@chemeng.osakafu-u.ac.jp.
authors: Toshiyuki
authors: T
authors: Nomura
authors: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors: Shuji
authors: S
authors: Tani
authors: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors: Makoto
authors: M
authors: Yamamoto
authors: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors: Takumi
authors: T
authors: Nakagawa
authors: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors: Shunsuke
authors: S
authors: Toyoda
authors: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors: Eri
authors: E
authors: Fujisawa
authors: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors: Akiko
authors: A
authors: Yasui
authors: Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan.
authors: Yasuhiro
authors: Y
authors: Konishi
mesh_terms: D004785:Environmental Pollutants / Q000737:chemistry / Q000633:toxicity*; D005658:Fungi / Q000187:drug effects*; D057927:Hydrophobic and Hydrophilic Interactions; D007552:Isotonic Solutions; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D011137:Polystyrenes / Q000737:chemistry / Q000633:toxicity*; D013499:Surface Properties; D018675:Toxicity Tests
chemical_list: D004785:Environmental Pollutants; D007552:Isotonic Solutions; D011137:Polystyrenes; C024075:styrofoam
keywords: Adhesion; Cytotoxicity; Filamentous fungi; Hydrophobin; Polystyrene latex nanoparticles; Uptake