title: Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF-α and IL-1β.
abstract: The present study aimed to explore the molecular mechanisms associated with intervertebral disc degeneration (IDD) induced by tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β. The microarray dataset no. GSE42611 was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) between four experimental nucleus pulposus samples and four control nucleus pulposus samples were analyzed. Subsequently, Gene Ontology (GO) and pathway enrichment analyses of DEGs were performed, followed by protein‑protein interaction (PPI) network construction and prediction of a regulatory network of transcription factor (TFs). Finally, the transcriptional regulatory network was integrated into the PPI network to analyze the network modules. A total of 246 upregulated and 290 downregulated DEGs were identified. The upregulated DEGs were mainly associated with GO terms linked with inflammatory response and apoptotic pathways, while the downregulated DEGs were mainly associated with GO terms linked with cell adhesion and pathways of extracellular matrix ‑ receptor interaction. In the PPI network, IL6, COL1A1, NFKB1 and HIF1A were hub genes, and in addition, NFKB1 and HIF1A were TFs. Pathways of apoptosis and extracellular matrix ‑ receptor interaction may have important roles in IDD progression. IL6, COL1A1 and the TFs NFKB1 and HIF1A may be used as biomarkers for IDD diagnosis and treatment.
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Feng
authors: F
authors: Xu
authors: Department of Orthopaedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors: Feng
authors: F
authors: Gao
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Yadong
authors: Y
authors: Liu
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Zhenyu
authors: Z
authors: Wang
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Xinming
authors: X
authors: Zhuang
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Zhigang
authors: Z
authors: Qu
authors: Teaching and Research Department of Health Service Management, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China.
authors: Hui
authors: H
authors: Ma
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Yi
authors: Y
authors: Liu
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Changfeng
authors: C
authors: Fu
authors: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Qi
authors: Q
authors: Zhang
authors: Department of Acupuncture and Moxibustion, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors: Xiaoying
authors: X
authors: Duan
mesh_terms: D019295:Computational Biology; D020869:Gene Expression Profiling; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D006801:Humans; D053583:Interleukin-1beta / Q000502:physiology*; D055959:Intervertebral Disc Degeneration / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps; D014157:Transcription Factors / Q000502:physiology; D059467:Transcriptome; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D053583:Interleukin-1beta; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha