title: Artemia salina as a new index for assessment of acute cytotoxicity during co-composting of sewage sludge and lignocellulose waste.
abstract: Considering the necessity to constantly monitor the safety of use of sewage sludge, we have focused on evaluating the toxicity of raw sludge and sludge treated by co-composting with date palm waste using an in vitro assessment of cytotoxicity based on Artemia salina larvae as a simple new sensitive and reliable routine test. The efficiency of co-composting in decreasing sludge toxicity was evaluated in terms of cytotoxicity abatement reaching 100% by the second month of composting for mixture A (1/3 sludge+2/3 date palm waste) and the third month for mixture B (1/2 sludge+1/2 date palm waste). Cytotoxicity abatement was confirmed by the increase of germination index, which reached over 100% with positive correlation for lettuce (R(2)=0.81 and 0.86) and for turnip (R(2)=0.87 and 0.74) for mixtures A and B respectively. A strong correlation between the proposed cytotoxicity test and the evolution of regulatory physical-chemical approaches was found, (R(2)=0.88 and 0.89) for NH4(+)/NO3(-) and (R(2)=0.80 and 0.88) for C/N respectively for mixture A and B. These findings allow the inexpensive bioassay reported to be used as a highly sensitive test to determine the cytotoxicity and maturity of composts.
authors: Laboratory of Ecology and Environment (L2E) (Unit Associated with the CNRST, URAC32), Faculty of Science Semlalia, Cadi Ayyad University, BP: 2390 Marrakech, Morocco. Electronic address: loubna.elfels@gmail.com.
authors: Loubna
authors: L
authors: El Fels
authors: Laboratory of Ecology and Environment (L2E) (Unit Associated with the CNRST, URAC32), Faculty of Science Semlalia, Cadi Ayyad University, BP: 2390 Marrakech, Morocco.
authors: Mohamed
authors: M
authors: Hafidi
authors: Laboratory of Biology and Biotechnology of Microorganisms, School of Science Semlalia, Cadi Ayyad University, BP: 2390 Marrakech, Morocco.
authors: Yedir
authors: Y
authors: Ouhdouch
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects*; D003603:Cytotoxins / Q000633:toxicity*; D008031:Lignin / Q000633:toxicity*; D009018:Morocco; D012722:Sewage / Q000032:analysis*; D014865:Waste Disposal, Fluid
chemical_list: D003603:Cytotoxins; D012722:Sewage; C036909:lignocellulose; D008031:Lignin
keywords: Artemia salina bioassay; Compost maturity; Cytotoxicity; Phytotoxity; Sludge