title: Detection and characterization of diverse alpha- and betacoronaviruses from bats in China.
abstract: Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses (CoVs) were detected in 50 of 951 (positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase (RdRP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus. Among the 50 positive specimens, thirteen gave rise to CoV full-length RdRP gene amplification for further sequence comparison, of which three divergent sequences (two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses (JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus (JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed.
authors: Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China.
authors: Lin
authors: L
authors: Xu
authors: Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
authors: Fuqiang
authors: F
authors: Zhang
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
authors: Weihong
authors: W
authors: Yang
authors: School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China.
authors: Tinglei
authors: T
authors: Jiang
authors: School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China.
authors: Guanjun
authors: G
authors: Lu
authors: Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China.
authors: Biao
authors: B
authors: He
authors: Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China.
authors: Xingyu
authors: X
authors: Li
authors: Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
authors: Tingsong
authors: T
authors: Hu
authors: Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
authors: Gang
authors: G
authors: Chen
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
authors: Yun
authors: Y
authors: Feng
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
authors: Yuzhen
authors: Y
authors: Zhang
authors: Center for Disease Control and Prevention of Chengdu Military Region, Kunming, 650118, China.
authors: Quanshui
authors: Q
authors: Fan
authors: School of Urban and Environmental Sciences, Northeast Normal University, Changchun, 130024, China.
authors: Jiang
authors: J
authors: Feng
authors: Yunnan Institute of Endemic Diseases Control and Prevention, Dali, 671000, China.
authors: Hailin
authors: H
authors: Zhang
authors: Military Veterinary Institute, Academy of Military Medical Sciences, Changchun, 130122, China. changchun_tu@hotmail.com.|Jiangsu Co-innovation Center for Prevention and Control of Important, Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. changchun_tu@hotmail.com.
authors: Changchun
authors: C
authors: Tu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D002685:Chiroptera / Q000821:virology*; D017934:Coronavirus / Q000145:classification* / Q000235:genetics; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D010802:Phylogeny; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D017421:Sequence Analysis
chemical_list: D012324:RNA-Dependent RNA Polymerase
keywords: Alphacoronavirus; Betacoronavirus; bats; diversity