title: Transport physics and biorheology in the setting of hemostasis and thrombosis.
abstract: The biophysics of blood flow can dictate the function of molecules and cells in the vasculature with consequent effects on hemostasis, thrombosis, embolism, and fibrinolysis. Flow and transport dynamics are distinct for (i) hemostasis vs. thrombosis and (ii) venous vs. arterial episodes. Intraclot transport changes dramatically the moment hemostasis is achieved or the moment a thrombus becomes fully occlusive. With platelet concentrations that are 50- to 200-fold greater than platelet-rich plasma, clots formed under flow have a different composition and structure compared with blood clotted statically in a tube. The platelet-rich, core/shell architecture is a prominent feature of self-limiting hemostatic clots formed under flow. Importantly, a critical threshold concentration of surface tissue factor is required for fibrin generation under flow. Once initiated by wall-derived tissue factor, thrombin generation and its spatial propagation within a clot can be modulated by γ'-fibrinogen incorporated into fibrin, engageability of activated factor (FIXa)/activated FVIIIa tenase within the clot, platelet-derived polyphosphate, transclot permeation, and reduction of porosity via platelet retraction. Fibrin imparts tremendous strength to a thrombus to resist embolism up to wall shear stresses of 2400 dyne cm(-2) . Extreme flows, as found in severe vessel stenosis or in mechanical assist devices, can cause von Willebrand factor self-association into massive fibers along with shear-induced platelet activation. Pathological von Willebrand factor fibers are A Disintegrin And Metalloprotease with ThromboSpondin-1 domain 13 resistant but are a substrate for fibrin generation due to FXIIa capture. Recently, microfluidic technologies have enhanced the ability to interrogate blood in the context of stenotic flows, acquired von Willebrand disease, hemophilia, traumatic bleeding, and drug action.
authors: Departments of Medicine and Systems Pharmacology, University of Pennsylvania, Philadelphia, PA, USA.
authors: L F
authors: LF
authors: Brass
authors: Institute for Medicine and Engineering, Department of Chemical Engineering, Philadelphia, PA, USA.
authors: S L
authors: SL
authors: Diamond
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001783:Blood Flow Velocity; D001792:Blood Platelets / Q000187:drug effects; D003251:Constriction, Pathologic; D004058:Diffusion; D015949:Factor IXa / Q000737:chemistry; D015944:Factor VIIIa / Q000737:chemistry; D005337:Fibrin / Q000737:chemistry; D005342:Fibrinolysis; D006487:Hemostasis; D006801:Humans; D051379:Mice; D015539:Platelet Activation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D053657:Platelet-Rich Plasma / Q000378:metabolism; D011122:Polyphosphates / Q000737:chemistry; D016062:Porosity; D012212:Rheology; D013314:Stress, Mechanical; D013917:Thrombin / Q000494:pharmacology; D013925:Thromboplastin / Q000494:pharmacology; D013927:Thrombosis / Q000503:physiopathology*; D014841:von Willebrand Factor / Q000737:chemistry
chemical_list: D011122:Polyphosphates; D014841:von Willebrand Factor; D015944:Factor VIIIa; D005337:Fibrin; D013925:Thromboplastin; D015949:Factor IXa; D013917:Thrombin
keywords: fibrin; hemodynamics; platelet; shear stress; thrombin; von Willebrand factor