title: An in vitro diagnosis of oral premalignant lesion using time-resolved fluorescence spectroscopy under UV excitation-a pilot study.
abstract: In spite of rapid advancement in cancer treatment, early diagnosis of cancer and medicable precursors are still the finest approach towards the assurance of patient lives and enhancement in the quality of their life. In this regard, the present study deals with the time-resolved fluorescence spectroscopy of normal and premalignant oral tissues under UV excitations (280nm and 310nm). The decay kinetics at 350nm emission of normal tissues exhibit higher fluorescence lifetime than that of premalignant tissues and subsequent statistical analysis shows that the data were statistically significant. Further, the decay kinetics at 450nm emission for normal and premalignant oral tissues was obtained. Subsequently, statistical analysis revealed that except fast component, rest of the component lifetimes and fractional amplitudes were not statistically significant. An attempt has also been made to explore the better statistical tool to discriminate premalignant tissues from normal ones at 350nm emission. Among stepwise linear discriminant analysis (SLDA) and receiver operator characteristics (ROC), the former discriminates premalignant from normal tissues with 86.7% specificity and 93.3% sensitivity. Hence, fluorescence lifetime spectroscopy at 350nm emission opens a new avenue for early detection of oral cancer.
authors: Department of Medical Physics, Anna University, Chennai 600 025, India. Electronic address: kudayakumar85@gmail.com.
authors: Udayakumar
authors: U
authors: Kanniyappan
authors: Department of Medical Physics, Anna University, Chennai 600 025, India.
authors: Aruna
authors: A
authors: Prakasarao
authors: Department of Oral Medicine and Radiology, Meenakshi Ammal Dental College and Hospital, Chennai 600 095, India.
authors: Koteeswaran
authors: K
authors: Dornadula
authors: Department of Medical Physics, Anna University, Chennai 600 025, India. Electronic address: ganesansingaravelu@gmail.com.
authors: Ganesan
authors: G
authors: Singaravelu
mesh_terms: D016002:Discriminant Analysis; D055088:Early Detection of Cancer / Q000379:methods* / Q000592:standards; D005453:Fluorescence; D006801:Humans; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000473:pathology; D010865:Pilot Projects; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000473:pathology; D012372:ROC Curve; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
keywords: Discriminant analysis; Fluorescence; Fluorescence lifetime; Premalignant tissue