title: The Photoheterotrophic Growth of Bacteriochlorophyll Synthase-Deficient Mutant of Rhodobacter sphaeroides Is Restored by I44F Mutant Chlorophyll Synthase of Synechocystis sp. PCC 6803.
abstract: Chlorophyll synthase (ChlG) and bacteriochlorophyll synthase (BchG) have a high degree of substrate specificity. The BchG mutant of Rhodobacter sphaeroides, BG1 strain, is photosynthetically incompetent. When BG1 harboring chlG of Synechocystis sp. PCC 6803 was cultured photoheterotrophically, colonies arose at a frequency of approximately 10(-8). All the suppressor mutants were determined to have the same mutational change, ChlGI44F. The mutated enzyme ChlGI44F showed BchG activity. Remarkably, BchGF28I, which has the substitution of F at the corresponding 28(th) residue to I, showed ChlG activity. The Km values of ChlGI44F and BchGF28I for their original substrates, chlorophyllide (Chlide) a and bacteriochlorophyllide (Bchlide) a, respectively, were not affected by the mutations, but the Km values of ChlGI44F and BchGF28I for the new substrates Bchlide a and Chlide a, respectively, were more than 10-fold larger than those for their original substrates, suggesting the lower affinities for new substrates. Taken together, I44 and F28 are important for the substrate specificities of ChlG and BchG, respectively. The BchG activity of ChlGI44F and the ChlG activity of BchGF28I further suggest that ChlG and BchG are evolutionarily related enzymes.
authors: Department of Life Science, Sogang University, Seoul 04107, Republic of Korea.
authors: Eui-Jin
authors: EJ
authors: Kim
authors: Department of Life Science, Sogang University, Seoul 04107, Republic of Korea.
authors: Hyeonjun
authors: H
authors: Kim
authors: Department of Life Science, Sogang University, Seoul 04107, Republic of Korea.
authors: Jeong K
authors: JK
authors: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D025541:Bacteriochlorophyll A / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D019729:Carbon-Oxygen Ligases / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004789:Enzyme Activation; D052836:Heterotrophic Processes; D016297:Mutagenesis, Site-Directed; D010788:Photosynthesis; D052817:Phototrophic Processes; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D013379:Substrate Specificity; D046939:Synechocystis / Q000201:enzymology* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D025541:Bacteriochlorophyll A; D002734:Chlorophyll; D019729:Carbon-Oxygen Ligases; C105512:bacteriochlorophyll a synthase
keywords: Rhodobacter sphaeroides; Synechocystis sp. PCC 6803; bacteriochlorophyll synthase; chlorophyll synthase