title: Virulence of representative Japanese Francisella tularensis and immunologic consequences of infection in mice.
abstract: Francisella tularensis, which causes tularemia, is widely distributed in the Northern hemisphere. F. tularensis strains isolated in Japan are genetically unique from non-Japanese strains; however, their phenotypic properties have not been well studied. Thus, mice were infected with representative Japanese strains of F. tularensis and their virulence and mouse immune responses to them assessed. Of four representative Japanese strains, the Ebina, Jap and Tsuchiya strains were susceptible to H2 O2 and did not grow well intracellularly. Only Yama strain grew intracellularly and was lethal to mice. Infection with Yama strain resulted in drastic increases in IFN-γ, CD4 and CD8 double-positive T cells and Th1 cells (CD3, CD4 and Tim3-positive cells), and a decrease in the ratio of CD8-positive CD4-negative T cells in mice. C57BL/6J mice that survived infection produced IgM antibodies to LPS and IgG2c antibodies to 43, 19 and 17 kDa proteinase K-sensitive components. These data are valuable for understanding the phenotypic properties of F. tularensis in Japan.
authors: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640.
authors: Akitoyo
authors: A
authors: Hotta
authors: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640.
authors: Osamu
authors: O
authors: Fujita
authors: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640.
authors: Akihiko
authors: A
authors: Uda
authors: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640.
authors: Yoshie
authors: Y
authors: Yamamoto
authors: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640.|United Graduate School of Veterinary Science Gifu University, 1-1 Yanagido, Gifu 501-1193.
authors: Neekun
authors: N
authors: Sharma
authors: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640.
authors: Kiyoshi
authors: K
authors: Tanabayashi
authors: Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
authors: Akio
authors: A
authors: Yamada
authors: Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640.|United Graduate School of Veterinary Science Gifu University, 1-1 Yanagido, Gifu 501-1193.
authors: Shigeru
authors: S
authors: Morikawa
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D005604:Francisella tularensis / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D007564:Japan; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014406:Tularemia / Q000276:immunology* / Q000382:microbiology*; D014774:Virulence
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D051792:Basic Helix-Loop-Helix Proteins; D016207:Cytokines; D006861:Hydrogen Peroxide; D007075:Immunoglobulin M; D007371:Interferon-gamma; C095572:Hand1 protein, mouse; C000611649:IFNG protein, mouse
keywords: Francisella tularensis; Japan; immune response; virulence