title: Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture.
abstract: Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, increased activity of TGF-β1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-β1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-β1 and a range of pro-fibrotic extracellular matrix genes.
authors: Regenerative, Modular &Developmental Engineering Laboratory (REMODEL), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.|Centre for Research in Medical Devices (CÚRAM), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
authors: Sunny A
authors: SA
authors: Abbah
authors: Centre for Research in Medical Devices (CÚRAM), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.|Regenerative Medicine Institute (REMEDI), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
authors: Dilip
authors: D
authors: Thomas
authors: Centre for Research in Medical Devices (CÚRAM), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
authors: Shane
authors: S
authors: Browne
authors: Centre for Research in Medical Devices (CÚRAM), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.|Regenerative Medicine Institute (REMEDI), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
authors: Timothy
authors: T
authors: O'Brien
authors: Centre for Research in Medical Devices (CÚRAM), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
authors: Abhay
authors: A
authors: Pandit
authors: Regenerative, Modular &Developmental Engineering Laboratory (REMODEL), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.|Centre for Research in Medical Devices (CÚRAM), Biosciences Research Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
authors: Dimitrios I
authors: DI
authors: Zeugolis
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D058575:Decorin / Q000235:genetics* / Q000378:metabolism; D004549:Elastin / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005355:Fibrosis; D005786:Gene Expression Regulation; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D013710:Tendons / Q000378:metabolism / Q000473:pathology; D000070916:Tenocytes / Q000378:metabolism*; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D058575:Decorin; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D003094:Collagen; D004549:Elastin