title: Expression of serotonin receptor genes in cranial ganglia.
abstract: Taste cells release neurotransmitters to gustatory neurons to transmit chemical information they received. Sweet, umami, and bitter taste cells use ATP as a neurotransmitter. However, ATP release from sour taste cells has not been observed so far. Instead, they release serotonin when they are activated by sour/acid stimuli. Thus it is still controversial whether sour taste cells use ATP, serotonin, or both. By reverse transcription-polymerase chain reaction and subsequent in situ hybridization (ISH) analyses, we revealed that of 14 serotonin receptor genes only 5-HT3A and 5-HT3B showed significant/clear signals in a subset of neurons of cranial sensory ganglia in which gustatory neurons reside. Double-fluorescent labeling analyses of ISH for serotonin receptor genes with wheat germ agglutinin (WGA) in cranial sensory ganglia of pkd1l3-WGA mice whose sour neural pathway is visualized by the distribution of WGA originating from sour taste cells in the posterior region of the tongue revealed that WGA-positive cranial sensory neurons rarely express either of serotonin receptor gene. These results suggest that serotonin receptors expressed in cranial sensory neurons do not play any role as neurotransmitter receptor from sour taste cells.
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Naohiro
authors: N
authors: Maeda
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Monell Chemical Senses Center, Philadelphia, PA, USA.
authors: Makoto
authors: M
authors: Ohmoto
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Kurumi
authors: K
authors: Yamamoto
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Azusa
authors: A
authors: Kurokawa
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Masataka
authors: M
authors: Narukawa
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Yoshiro
authors: Y
authors: Ishimaru
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Takumi
authors: T
authors: Misaka
authors: Monell Chemical Senses Center, Philadelphia, PA, USA.
authors: Ichiro
authors: I
authors: Matsumoto
authors: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan; Kanagawa Academy of Science and Technology, Takatsu-ku, Kawasaki-shi, Kanagawa, Japan. Electronic address: aka7308@mail.ecc.u-tokyo.ac.jp.
authors: Keiko
authors: K
authors: Abe
mesh_terms: D000818:Animals; D017950:Ganglia, Sensory / Q000378:metabolism*; D015870:Gene Expression; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D058476:Receptors, Purinergic P2X2 / Q000235:genetics / Q000378:metabolism; D011985:Receptors, Serotonin / Q000235:genetics / Q000378:metabolism*; D044406:Receptors, Serotonin, 5-HT3 / Q000235:genetics / Q000378:metabolism; D011984:Sensory Receptor Cells / Q000378:metabolism; D012886:Skull / Q000294:innervation*; D013649:Taste
chemical_list: D058476:Receptors, Purinergic P2X2; D011985:Receptors, Serotonin; D044406:Receptors, Serotonin, 5-HT3
keywords: Geniculate ganglion; Nodose/petrosal ganglia; Serotonin receptor; Taste