title: Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms.
abstract: The moss Physcomitrella patens possesses highly efficient homologous recombination allowing targeted gene manipulations and displays many features of the early land plants including high tolerance to abiotic stresses. It is therefore an invaluable model organism for studies of gene functions and comparative studies of evolution of stress responses in plants. Here, we describe a method for generating targeted gene knockout lines in P. patens using a polyethylene glycol-mediated transformation of protoplasts including basic in vitro growth, propagation, and maintenance techniques.
authors: Max F. Perutz Laboratories, Medical University of Vienna, Dr. Bohrgasse 9, Vienna, 1030, Austria.
authors: Monika
authors: M
authors: Maronova
authors: Institute of Applied Genetics and Cell Biology (IAGZ), BOKU - University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, 1190, Austria. mariya.kalyna@boku.ac.at.
authors: Maria
authors: M
authors: Kalyna
mesh_terms: D019068:Bryopsida / Q000235:genetics / Q000378:metabolism; D055786:Gene Knockout Techniques / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism
keywords: Evolution; Gene targeting; Homologous recombination; Knockout; Physcomitrella; Plant; Protoplast; Stress