title: Neutrophil Migration into the Infected Uroepithelium Is Regulated by the Crosstalk between Resident and Helper Macrophages.
abstract: The antibacterial defense against infections depends on the cooperation between distinct phagocytes of the innate immune system, namely macrophages and neutrophils. However, the mechanisms driving this cooperation are incompletely understood. In this study we describe the crosstalk between Ly6C⁺ and Ly6C(-) macrophage-subtypes and neutrophils in the context of urinary tract infection (UTI) with uropathogenic E. coli (UPEC). Ly6C(-) macrophages acted as tissue resident sentinels and attracted circulating phagocytes by chemokines. Ly6C⁺ macrophages produced tumor necrosis factor (TNF) that licensed Ly6C(-) macrophages to release preformed CXCL2, which in turn caused matrix metalloproteinases (MMP-9) secretion by neutrophils to enable transepithelial migration.
authors: Institute of Experimental Immunology and Imaging, Department Immunodynamics, University Hospital Essen, University Duisburg-Essen, Essen 45141, Germany. kristina.zec@uk-essen.de.
authors: Kristina
authors: K
authors: Zec
authors: Institute of Experimental Immunology and Imaging, Department Immunodynamics, University Hospital Essen, University Duisburg-Essen, Essen 45141, Germany. julia.volke@stud.uni-due.de.
authors: Julia
authors: J
authors: Volke
authors: Institute of Experimental Immunology and Imaging, Department Immunodynamics, University Hospital Essen, University Duisburg-Essen, Essen 45141, Germany. nirojah.vijitha@uk-essen.de.
authors: Nirojah
authors: N
authors: Vijitha
authors: Institute of Experimental Immunology and Imaging, Department Immunodynamics, University Hospital Essen, University Duisburg-Essen, Essen 45141, Germany. thiebes@immunodynamics.de.
authors: Stephanie
authors: S
authors: Thiebes
authors: Institute of Experimental Immunology and Imaging, Department Immunodynamics, University Hospital Essen, University Duisburg-Essen, Essen 45141, Germany. Matthias.Gunzer@uni-due.de.
authors: Matthias
authors: M
authors: Gunzer
authors: Institute of Experimental Immunology, University Hospital Bonn, Bonn 53127, Germany. ckurts@uni-bonn.de.
authors: Christian
authors: C
authors: Kurts
authors: Institute of Experimental Immunology and Imaging, Department Immunodynamics, University Hospital Essen, University Duisburg-Essen, Essen 45141, Germany. engel@immunodynamics.de.
authors: Daniel Robert
authors: DR
authors: Engel
keywords: Ly6C+ and Ly6C− macrophages; crosstalk; neutrophils; transepithelial migration; uropathogenic E. coli (UPEC)