title: MC3T3-E1 Cell Response to Ti1-xAgx and Ag-TiNx Electrodes Deposited on Piezoelectric Poly(vinylidene fluoride) Substrates for Sensor Applications.
abstract: In the sensors field, titanium based coatings are being used for the acquisition/application of electrical signals from/to piezoelectric materials. In this particular case, sensors are used to detect dynamic mechanical loads at early stages after intervention of problems associated with prostheses implantation. The aim of this work is to select an adequate electrode for sensor applications capable, in an initial stage to avoid bone cell adhesion, but at a long stage, permit osteointegration and osteoinduction. This work reports on the evaluation of osteoblast MC3T3-E1 cells behavior in terms of proliferation, adhesion and long-term differentiation of two different systems used as sensor electrodes: Ti1-xAgx and Ag-TiNx deposited by d.c. and pulsed magnetron sputtering at room temperature on poly(vinylidene fluoride) (PVDF). The results indicated an improved effect of Ag-TiNx electrodes compared with Ti1-xAgx and TiN, in terms of diminished cell adhesion and proliferation at an initial cell culture stage. Nevertheless, when cell culture time is longer, cells grown onto Ag-TiNx electrodes are capable to proliferate and also differentiate at proper rates, indicating the suitability of this coating for sensor application in prostheses devices. Thus, the Ag-TiNx system was considered the most promising electrode for tissue engineering applications in the design of sensors for prostheses to detect dynamic mechanical loads.
authors: GRF-CFUM, Physics Department, University of Minho , 4800-058 Guimarães, Portugal.
authors: S M
authors: SM
authors: Marques
authors: Center for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València , València, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , València, Spain.
authors: P
authors: P
authors: Rico
authors: GRF-CFUM, Physics Department, University of Minho , 4800-058 Guimarães, Portugal.
authors: I
authors: I
authors: Carvalho
authors: Center for Biomaterials and Tissue Engineering, CBIT, Universitat Politècnica de València , València, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) , València, Spain.
authors: J L
authors: JL
authors: Gómez Ribelles
authors: GRF-CFUM, Physics Department, University of Minho , 4800-058 Guimarães, Portugal.
authors: L
authors: L
authors: Fialho
authors: BCMaterials, Parque Científico y Tecnológico de Bizkaia , 48160 Derio, Spain.
authors: S
authors: S
authors: Lanceros-Méndez
authors: M
authors: M
authors: Henriques
authors: GRF-CFUM, Physics Department, University of Minho , 4800-058 Guimarães, Portugal.|SEG-CEMUC Mechanical Engineering Department, University of Coimbra , 3030-788 Coimbra, Portugal.
authors: S
authors: S
authors: Carvalho
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004566:Electrodes; D008422:Materials Testing; D051379:Mice; D011145:Polyvinyls / Q000737:chemistry / Q000494:pharmacology; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology; D005465:Fluorocarbon Polymers
chemical_list: D011145:Polyvinyls; D012834:Silver; D014025:Titanium; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
keywords: Ag nanoparticles; MC3T3 osteoblastic cells; piezoelectric polymers; sensors; sputtering