title: Association of RUNX3 Methylation with Clinical Outcome and Cell Migration/Invasion in Laryngeal Squamous Cell Carcinoma.
abstract: This study identifies promoter methylation status of RUNX3 in 77 LSCC patient tissues and their paired adjacent healthy tissues. Hypermethylation percentage RUNX3 occurred in LSCC samples was significantly higher than that in normal tissues, as well as associated with suppression of RUNX3 expression, TNM classification of malignant tumors stage, lymph node metastasis and poor overall survival rate. Reduced methylation and increased expression of RUNX3 genes in vitro was observed and decreased cell migration was further confirmed following 5-azacytidine treatment. RUNX 3 promoter hypermethylation can lead to down-regulation of RUNX3 in LSCC cancerous tissue.
authors: a Department of Otolaryngology, Head, and Neck Surgery , the Second Xiangya Hospital of Central South University , Hunan , China.|b Department of Otolaryngology, Head, and Neck Surgery , the Affiliated Hospital of Zunyi Medical College , Guizhou , China.
authors: Zhao-Hui
authors: ZH
authors: Liu
authors: a Department of Otolaryngology, Head, and Neck Surgery , the Second Xiangya Hospital of Central South University , Hunan , China.
authors: Jia-Jia
authors: JJ
authors: Liu
authors: a Department of Otolaryngology, Head, and Neck Surgery , the Second Xiangya Hospital of Central South University , Hunan , China.
authors: Shi-Sheng
authors: SS
authors: Li
authors: a Department of Otolaryngology, Head, and Neck Surgery , the Second Xiangya Hospital of Central South University , Hunan , China.
authors: Xin-Ming
authors: XM
authors: Yang
mesh_terms: D000368:Aged; D001374:Azacitidine / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000187:drug effects; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck; D013997:Time Factors
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; C493729:Runx3 protein, human; D015254:DNA Modification Methylases; D001374:Azacitidine
keywords: Cell migration; DNA methylation; Expression; Laryngeal squamous cell carcinoma; Runt-related transcription factor