title: Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis.
abstract: Major urinary proteins (MUPs) are the most abundant protein species in mouse urine, accounting for more than 90% of total protein content. Twenty-one Mup genes and 21 pseudogenes are clustered in a region of around 2 megabase pairs (Mbp) on chromosome 4. A Mup-knockout mouse model would greatly facilitate researches in the field of proteomic analysis of mouse urine. Here, we report the successful knockout of the Mup gene cluster of 2.2 Mbp using the CRISPR/Cas9 system. Homozygous Mup-knockout mice survived to adulthood and exhibited no obvious defects. The patterns of the proteomes of non-MUP urinary proteins in homozygous Mup-knockout mice were similar to those of wild-type mice judged by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The sensitivity of enzyme-linked immunosorbent assay to detect non-MUP urinary protein was significantly enhanced in Mup-knockout mice. In short, we have developed a Mup-knockout mouse model. This mouse model will be useful for the research of urinary biomarker testing that may have relevance for humans.
authors: College of Life Sciences, Beijing Normal University, Beijing 100875, China National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Haixia
authors: H
authors: Yang
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Wei
authors: W
authors: Zhang
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Shan
authors: S
authors: Lu
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Guangqing
authors: G
authors: Lu
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Hongjuan
authors: H
authors: Zhang
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Yinghua
authors: Y
authors: Zhuang
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Yue
authors: Y
authors: Wang
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Mengqiu
authors: M
authors: Dong
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China.
authors: Yu
authors: Y
authors: Zhang
authors: Beijing Ditan Hospital, Beijing 100015, China.
authors: Xingang
authors: X
authors: Zhou
authors: Beijing Ditan Hospital, Beijing 100015, China.
authors: Peng
authors: P
authors: Wang
authors: Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China chenliang@nibs.ac.cn yulei@sohu.com wangfengchao@nibs.ac.cn.
authors: Lei
authors: L
authors: Yu
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China chenliang@nibs.ac.cn yulei@sohu.com wangfengchao@nibs.ac.cn.
authors: Fengchao
authors: F
authors: Wang
authors: National Institute of Biological Sciences, Beijing, Beijing 102206, China National Institute of Biological Sciences, Collaborative Innovation Center for Cancer Medicine, Beijing 102206, China chenliang@nibs.ac.cn yulei@sohu.com wangfengchao@nibs.ac.cn.
authors: Liang
authors: L
authors: Chen
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D005810:Multigene Family; D010641:Phenotype; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics
chemical_list: D011506:Proteins; C035737:major urinary proteins
keywords: CRISPR/Cas9; genetically engineered mouse model; knockout; major urinary protein; urine