title: Pesticide analysis using nanoceria-coated paper-based devices as a detection platform.
abstract: We report the first use of a paper-based device coated with nanoceria as a simple, low-cost and rapid detection platform for the analysis of organophosphate (OP) pesticides using an enzyme inhibition assay with acetylcholinesterase (AChE) and choline oxidase (ChOX). In the presence of acetylcholine, AChE and ChOX catalyze the formation of H2O2, which is detected colorimetrically by a nanoceria-coated device resulting in the formation of a yellow color. After incubation with OP pesticides, the AChE activity was inhibited, producing less H2O2, and a reduction in the yellow intensity. The assay is able to analyze OP pesticides without the use of sophisticated instruments and gives detection limits of 18 ng mL(-1) and 5.3 ng mL(-1) for methyl-paraoxon and chlorpyrifos-oxon, respectively. The developed method was successfully applied to detect methyl-paraoxon in spiked vegetables (cabbage) and a dried seafood product (dried green mussel), obtaining ∼95% recovery values for both sample types. The spiked samples were also analyzed using LC-MS/MS as a comparison to the developed method and similar values were obtained, indicating that the developed method gives accurate results and is suitable for OP analysis in real samples.
authors: Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand. yupaporn@buu.ac.th and Savannakhet Teacher Training College, Savannakhet, Lao PDR.
authors: Souksanh
authors: S
authors: Nouanthavong
authors: Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
authors: Duangjai
authors: D
authors: Nacapricha
authors: Department of Chemistry, Colorado State University, Fort Collins, 80523-1872, USA.
authors: Charles S
authors: CS
authors: Henry
authors: Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Burapha University, Chon Buri, 20131, Thailand. yupaporn@buu.ac.th.
authors: Yupaporn
authors: Y
authors: Sameenoi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002563:Cerium / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000494:pharmacology; D003116:Color; D003124:Colorimetry; D004785:Environmental Pollutants / Q000032:analysis* / Q000494:pharmacology; D005504:Food Analysis; D006861:Hydrogen Peroxide / Q000378:metabolism; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D010209:Paper; D010575:Pesticides / Q000032:analysis* / Q000494:pharmacology
chemical_list: D002800:Cholinesterase Inhibitors; D004785:Environmental Pollutants; D010575:Pesticides; D002563:Cerium; C030583:ceric oxide; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase