title: Non-coding RNA LINC00857 is predictive of poor patient survival and promotes tumor progression via cell cycle regulation in lung cancer.
abstract: We employed next generation RNA sequencing analysis to reveal dysregulated long non-coding RNAs (lncRNAs) in lung cancer utilizing 461 lung adenocarcinomas (LUAD) and 156 normal lung tissues from 3 separate institutions. We identified 281 lncRNAs with significant differential-expression between LUAD and normal lung tissue. LINC00857, a top deregulated lncRNAs, was overexpressed in tumors and significantly associated with poor survival in LUAD. knockdown of LINC00857 with siRNAs decreased tumor cell proliferation, colony formation, migration and invasion in vitro, as well as tumor growth in vivo. Overexpression of LINC00857 increased cancer cell proliferation, colony formation and invasion. Mechanistic analyses indicated that LINC00857 mediates tumor progression via cell cycle regulation. Our study highlights the diagnostic/prognostic potential of LINC00857 in LUAD besides delineating the functional and mechanistic aspects of its aberrant disease specific expression and potentially using as a new therapeutic target.
authors: Guangxi Medical University, Nanning, China.|Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Lihui
authors: L
authors: Wang
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.|Guangzhou University of Chinese Medicine, Guangzhou, China.
authors: Yanli
authors: Y
authors: He
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.|The First People's Hospital of Yunnan Province, Kunming, China.
authors: Weijun
authors: W
authors: Liu
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.|Xinjiang Medical University, Xinjiang, China.
authors: Shengbin
authors: S
authors: Bai
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.|Xinjiang Medical University, Xinjiang, China.
authors: Lei
authors: L
authors: Xiao
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.|Xi'an Jiaotong University, Xi'an, China.
authors: Jie
authors: J
authors: Zhang
authors: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Saravana M
authors: SM
authors: Dhanasekaran
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Zhuwen
authors: Z
authors: Wang
authors: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Shanker
authors: S
authors: Kalyana-Sundaram
authors: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: O Alejandro
authors: OA
authors: Balbin
authors: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Sudhanshu
authors: S
authors: Shukla
authors: Guangxi Medical University, Nanning, China.
authors: Yi
authors: Y
authors: Lu
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Jules
authors: J
authors: Lin
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Rishindra M
authors: RM
authors: Reddy
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Philip W
authors: PW
authors: Carrott
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: William R
authors: WR
authors: Lynch
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Andrew C
authors: AC
authors: Chang
authors: Department of Pathology, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Arul M
authors: AM
authors: Chinnaiyan
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: David G
authors: DG
authors: Beer
authors: Guangxi Medical University, Nanning, China.
authors: Jian
authors: J
authors: Zhang
authors: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Michigan, United States of America.
authors: Guoan
authors: G
authors: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D016019:Survival Analysis; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C000625128:LINC00857 lnc, human; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm
keywords: LINC00857; diagnosis; lung adenocarcinoma; non-coding RNA; prognosis