title: Non-invasive detection of nasopharyngeal carcinoma using saliva surface-enhanced Raman spectroscopy.
abstract: The present study evaluated the use of saliva surface-enhanced Raman spectroscopy (SERS) for the detection of non-invasive nasopharyngeal carcinoma (NPC). SERS measurements were taken from 62 saliva samples, of which 32 were from NPC patients and 30 from healthy volunteers. Notable biochemical Raman bands in the SERS spectra were tentatively assigned to various saliva components. The saliva SERS spectra obtained from the NPC patients and the healthy volunteers were also analyzed by multivariate statistical techniques based on principal component analysis and linear discriminant analysis (PCA-LDA). Significant differences were observed between the saliva SERS spectral intensities for NPC patients and healthy volunteers, particularly at 447, 496, 635, 729, 1134, 1270 and 1448 cm-1, which primarily contained signals associated with proteins, nucleic acids, fatty acids, glycogen and collagen. The classification results based on the PCA-LDA method provided a relatively high diagnostic sensitivity of 86.7%, specificity of 81.3% and diagnostic accuracy of 83.9% for NPC identification. The results from the present study demonstrate that saliva SERS analysis used in conjunction with PCA-LDA diagnostic algorithms possesses a promising clinical application for the non-invasive detection of NPC.
authors: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Sufang
authors: S
authors: Qiu
authors: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Yuanji
authors: Y
authors: Xu
authors: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors: Lingling
authors: L
authors: Huang
authors: Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
authors: Wei
authors: W
authors: Zheng
authors: Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
authors: Chaobin
authors: C
authors: Huang
authors: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.
authors: Shaohua
authors: S
authors: Huang
authors: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.
authors: Jinyong
authors: J
authors: Lin
authors: College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
authors: Duo
authors: D
authors: Lin
authors: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.
authors: Shangyuan
authors: S
authors: Feng
authors: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China.
authors: Rong
authors: R
authors: Chen
authors: The Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China; Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
authors: Jianji
authors: J
authors: Pan
keywords: SERS; nasopharyngeal carcinoma; non-invasive detection; principal component analysis; saliva