title: Improving permeability and chromatographic performance of poly(pentaerythritol diacrylate monostearate) monolithic column via photo-induced thiol-acrylate polymerization.
abstract: A simple approach was developed for rapid preparation of polymeric monolithic columns in UV-transparent fused-silica capillaries via photoinitiated thiol-acrylate polymerization of pentaerythritol diacrylate monostearate (PEDAS) and trimethylolpropane tris(3-mercaptopropionate) (TPTM) within 10min, in which the acrylate homopolymerized and copolymerized with the thiol simultaneously. The morphology, permeability and chromatographic performance of the resulting poly(PEDAS-co-TPTM) monoliths were studied. It could be observed from SEM that the morphology of poly(PEDAS-co-TPTM) monolith was rather different from that of poly(PEDAS) monolith, which was fabricated via photo-induced free radical polymerization using PEDAS as the sole monomer. Compared with poly(PEDAS) monolith, poly(PEDAS-co-TPTM) monolith possessed better permeability when they were fabricated under the same preparation conditions. By adjusting the composition of porogenic solvents, poly(PEDAS-co-TPTM) monolith exhibited lower plate heights (15.7-17.7μm) than poly(PEDAS) monolith (19.1-37.9μm) in μLC. In addition, 66 unique peptides were positively identified on poly(PEDAS-co-TPTM) monolith when tryptic digest of four proteins was separated by μLC-MS/MS, demonstrating its potential in proteome analysis.
authors: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China; Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Hongwei
authors: H
authors: Wang
authors: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: junjieou@dicp.ac.cn.
authors: Junjie
authors: J
authors: Ou
authors: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Jingyao
authors: J
authors: Bai
authors: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Zhongshan
authors: Z
authors: Liu
authors: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Yating
authors: Y
authors: Yao
authors: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Lianfang
authors: L
authors: Chen
authors: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
authors: Xiaojun
authors: X
authors: Peng
authors: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: hanfazou@dicp.ac.cn.
authors: Hanfa
authors: H
authors: Zou
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000031:analogs & derivatives* / Q000737:chemistry*; D000179:Acrylates / Q000737:chemistry*; D002853:Chromatography, Liquid; D010455:Peptides / Q000032:analysis; D010539:Permeability; D055668:Photochemical Processes; D058105:Polymerization; D016062:Porosity; D011409:Propylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D013228:Stearates / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D000179:Acrylates; D010455:Peptides; D011409:Propylene Glycols; D011506:Proteins; D013228:Stearates; C531048:pentaerythritol diacrylate monostearate; C000611772:trimethylolpropane tris(3-mercaptopropionate; D015097:3-Mercaptopropionic Acid
keywords: 2,2-Dimethoxy-2-phenylacetophenone; Pentaerythritol diacrylate monostearate; Photoinitiated; Polymeric monoliths; Thiol-acrylate polymerization; Trimethylolpropane tris(3-mercaptopropionate)