title: Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum.
abstract: RNA helicases are indispensable for all organisms in each domain of life and have implications in numerous cellular processes. The DEAH-box RNA helicase Prp43 is involved in pre-mRNA splicing as well as rRNA maturation. Here, the crystal structure of Chaetomium thermophilum Prp43 at 2.9 Å resolution is revealed. Furthermore, it is demonstrated that Prp43 from C. thermophilum is capable of functionally replacing its orthologue from Saccharomyces cerevisiae in spliceosomal disassembly assays.
authors: Department of Molecular Structural Biology, Institute for Microbiology and Genetics, GZMB, Georg-August-Universität Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
authors: Marcel J
authors: MJ
authors: Tauchert
authors: Department of Cellular Biochemistry, Max Planck Institute of Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors: Jean-Baptiste
authors: JB
authors: Fourmann
authors: Department of Molecular Structural Biology, Institute for Microbiology and Genetics, GZMB, Georg-August-Universität Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
authors: Henning
authors: H
authors: Christian
authors: Department of Cellular Biochemistry, Max Planck Institute of Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors: Reinhard
authors: R
authors: Lührmann
authors: Department of Molecular Structural Biology, Institute for Microbiology and Genetics, GZMB, Georg-August-Universität Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
authors: Ralf
authors: R
authors: Ficner
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D002597:Chaetomium / Q000201:enzymology*; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods*; D053487:DEAD-box RNA Helicases / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D055550:Protein Stability; D020365:RNA Helicases / Q000737:chemistry* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D017381:Spliceosomes
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C109034:PRP43 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases
keywords: DEAH-box protein; DHX15; RNA helicase; spliceosome