title: Glycolipid analyses of light-harvesting chlorosomes from envelope protein mutants of Chlorobaculum tepidum.
abstract: Chlorosomes are large and efficient light-harvesting organelles in green photosynthetic bacteria, and they characteristically contain large numbers of bacteriochlorophyll c, d, or e molecules. Self-aggregated bacteriochlorophyll pigments are surrounded by a monolayer envelope membrane comprised of glycolipids and Csm proteins. Here, we analyzed glycolipid compositions of chlorosomes from the green sulfur bacterium Chlorobaculum tepidum mutants lacking one, two, or three Csm proteins by HPLC equipped with an evaporative light-scattering detector. The ratio of monogalactosyldiacylglyceride (MGDG) to rhamnosylgalactosyldiacylglyceride (RGDG) was smaller in chlorosomes from mutants lacking two or three proteins in CsmC/D/H motif family than in chlorosomes from the wild-type, whereas chlorosomes lacking CsmIJ showed relatively less RGDG than MGDG. The results suggest that the CsmC, CsmD, CsmH, and other chlorosome proteins are involved in organizing MGDG and RGDG and thereby affect the size and shape of the chlorosome.
authors: Earth-Life Science Institute, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro, Tokyo, 152-8550, Japan. tsukatani@elsi.jp.|PRESTO, Japan Science and Technology Agency, Saitama, 332-0012, Japan. tsukatani@elsi.jp.
authors: Yusuke
authors: http://orcid.org/0000-0003-2831-8996
authors: Y
authors: Tsukatani
authors: Graduate School of Life Sciences, Ritsumeikan University, Shiga, 525-8577, Japan.
authors: Tadashi
authors: T
authors: Mizoguchi
authors: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Pennsylvania, 16802, USA.
authors: Jennifer
authors: J
authors: Thweatt
authors: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Pennsylvania, 16802, USA.
authors: Marcus
authors: M
authors: Tank
authors: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Pennsylvania, 16802, USA.|Department of Chemistry and Biochemistry, Montana State University, Montana, 59717, USA.
authors: Donald A
authors: DA
authors: Bryant
authors: Graduate School of Life Sciences, Ritsumeikan University, Shiga, 525-8577, Japan.
authors: Hitoshi
authors: H
authors: Tamiaki
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000378:metabolism; D019414:Chlorobi / Q000235:genetics / Q000378:metabolism*; D038983:Galactolipids / Q000737:chemistry / Q000378:metabolism*; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D008027:Light; D008961:Models, Structural; D009154:Mutation; D015388:Organelles / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D038983:Galactolipids; D006017:Glycolipids; C475298:diacylgalactosylglycerol; C009909:monogalactosyldiacylglycerol
keywords: Bacteriochlorophyll; Chlorosome; Glycolipid; Green sulfur bacteria; Photosynthesis