title: Voltage-Gated K+ Channel, Kv3.3 Is Involved in Hemin-Induced K562 Differentiation.
abstract: Voltage-gated K+ (Kv) channels are well known to be involved in cell proliferation. However, even though cell proliferation is closely related to cell differentiation, the relationship between Kv channels and cell differentiation remains poorly investigated. This study demonstrates that Kv3.3 is involved in K562 cell erythroid differentiation. Down-regulation of Kv3.3 using siRNA-Kv3.3 increased hemin-induced K562 erythroid differentiation through decreased activation of signal molecules such as p38, cAMP response element-binding protein, and c-fos. Down-regulation of Kv3.3 also enhanced cell adhesion by increasing integrin β3 and this effect was amplified when the cells were cultured with fibronectin. The Kv channels, or at least Kv3.3, appear to be associated with cell differentiation; therefore, understanding the mechanisms of Kv channel regulation of cell differentiation would provide important information regarding vital cellular processes.
authors: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors: Min Seok
authors: MS
authors: Song
authors: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors: Seon Young
authors: SY
authors: Choi
authors: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors: Pan Dong
authors: PD
authors: Ryu
authors: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors: So Yeong
authors: SY
authors: Lee
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects* / Q000235:genetics; D005353:Fibronectins / Q000494:pharmacology; D006427:Hemin / Q000494:pharmacology*; D006801:Humans; D039661:Integrin beta3 / Q000096:biosynthesis; D020014:K562 Cells; D020218:Response Elements / Q000502:physiology*; D051670:Shaw Potassium Channels / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D005353:Fibronectins; C061248:ITGB3 protein, human; D039661:Integrin beta3; C489594:KCNC3 protein, human; D051670:Shaw Potassium Channels; D006427:Hemin