title: The Hydroxyl Radical is a Critical Intermediate in the Voltammetric Detection of Hydrogen Peroxide.
abstract: Cyclic voltammetry is a widely used and powerful tool for sensitively and selectively measuring hydrogen peroxide (H2O2). Herein, voltammetry was combined with electron paramagnetic resonance spectroscopy to identify and define the role of an oxygen-centered radical liberated during the oxidation of H2O2. The spin-trap reagents, 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide (EMPO), were employed. Spectra exhibit distinct hyperfine patterns that clearly identify the DMPO(•)-OH and EMPO(•)-OH adducts. Multiple linear regression analysis of voltammograms demonstrated that the hydroxyl radical is a principal contributor to the voltammetry of H2O2, as signal is attenuated when this species is trapped. These data incorporate a missing, fundamental element to our knowledge of the mechanisms that underlie H2O2 electrochemistry.
authors: Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.
authors: James G
authors: JG
authors: Roberts
authors: Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.
authors: Maxim A
authors: MA
authors: Voinov
authors: Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.
authors: Andreas C
authors: AC
authors: Schmidt
authors: Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.
authors: Tatyana I
authors: TI
authors: Smirnova
authors: Department of Chemistry, North Carolina State University , Raleigh, North Carolina 27695, United States.
authors: Leslie A
authors: LA
authors: Sombers
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000032:analysis / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry; D013113:Spin Labels
chemical_list: C406273:2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide; D011758:Pyrroles; D013113:Spin Labels; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide