title: Proteomic analysis of a compatible interaction between sugarcane and Sporisorium scitamineum.
abstract: Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. scitamineum infected susceptible sugarcane cultivar at whip emergence stage. Differentially abundant proteins were identified by 2DE coupled with MALDI-TOF/TOF-MS. Comprehensively, 53 sugarcane proteins identified were related to defence, stress, metabolism, protein folding, energy, and cell division; in addition, a putative effector of S. scitamineum, chorismate mutase, was identified. Transcript expression vis-à-vis the activity of phenylalanine ammonia lyase was relatively higher in the infected meristem. Abundance of seven candidate proteins in 2D gel profiles was in correlation with its corresponding transcript expression levels as validated by qRT-PCR. Furthermore, this study has opened up new perspectives on the interaction between sugarcane and S. scitamineum.
authors: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India.
authors: Leonard
authors: L
authors: Barnabas
authors: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India.
authors: N M R
authors: NM
authors: Ashwin
authors: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India.
authors: K
authors: K
authors: Kaverinathan
authors: Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy.
authors: Anna Rita
authors: AR
authors: Trentin
authors: Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy.
authors: Micaela
authors: M
authors: Pivato
authors: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India.
authors: A Ramesh
authors: AR
authors: Sundar
authors: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India.
authors: P
authors: P
authors: Malathi
authors: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India.
authors: R
authors: R
authors: Viswanathan
authors: Bioinformatics Center, ICAR-Indian Institute of Spices Research, Kozhikode, India.
authors: O B
authors: OB
authors: Rosana
authors: Bioinformatics Center, ICAR-Indian Institute of Spices Research, Kozhikode, India.
authors: K
authors: K
authors: Neethukrishna
authors: Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy.
authors: Paolo
authors: P
authors: Carletti
authors: Proteomics Center of Padova University, Padova, Italy.|Department of Biomedical Sciences, University of Padova, Padova, Italy.
authors: Giorgio
authors: G
authors: Arrigoni
authors: Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy.
authors: Antonio
authors: A
authors: Masi
authors: Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal.|GRADE (Global Research Arch for Developing Education) Academy Private Limited, Birgunj, Nepal.
authors: Ganesh Kumar
authors: GK
authors: Agrawal
authors: Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal.|GRADE (Global Research Arch for Developing Education) Academy Private Limited, Birgunj, Nepal.|Tsukuba International Academy for Sport Studies (TIAS) and Faculty of Health and Sport Sciences, University of Tsukuba, Ibaraki, Japan.
authors: Randeep
authors: R
authors: Rakwal
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D054884:Host-Pathogen Interactions; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D031786:Saccharum / Q000378:metabolism* / Q000382:microbiology*; D014587:Ustilaginales / Q000472:pathogenicity*
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Compatible interaction; Plant proteomics; Smut; Sporisorium scitamineum; Sugarcane