title: The Expression of MicroRNA-155 in Plasma and Tissue Is Matched in Human Laryngeal Squamous Cell Carcinoma.
abstract: OBJECTIVE
  Tumor-associated microRNAs have been detected in cancer, though whether plasma microRNA-155 (miR-155) could be a potential biomarker for laryngeal squamous cell carcinoma (LSCC) prognosis is unclear. We aimed to determine how miR-155 can be used to predict the clinical characteristics of patients with LSCC and correctly diagnose them.
  
  
  METHODS
  We collected tissue samples and peripheral blood samples before and after treatment from 280 LSCC cases and 560 controls. Real-time quantitative reverse transcription PCR was employed in this study to compare the relative expression of miR-155.
  
  
  RESULTS
  A total of 280 LSCC patients and 560 age- and sex-matched controls were included in the study. The miR-155 level was more up-regulated in LSCC tissue than in the non-tumor tissues (13.6 ± 2.4 vs. 3.1 ± 0.80, p<0.001). Additionally, a significantly higher miR-155 level in plasma samples from LSCC patients than in those of the controls (8.9 ± 1.25 vs. 1.8 ± 0.8, p<0.001) was reported. Tissue miR-155 showed an area under the curve (AUC) of 0.933, with a sensitivity of 82.6% and a specificity of 89.2%. The AUC for plasma miR-155 was 0.757, with a sensitivity of 58.4% and a specificity of 69.5%. When early LSCC in TNM I stage was considered, tissue miR-155 showed an area under the curve of 0.804, with a sensitivity of 85.2% and a specificity of 87.3%.
  
  
  CONCLUSIONS
  The expression of tissue and plasma miR-155 were significantly up-regulated in patients with LSCC. Our work will serve as a basis for further investigation, preferably large-scale validation in clinical trials.
authors: Department of Otolaryngol Head Neck Surgery, General Hospital of Tianjin Medical University, Tianjin, China.
authors: Jian Ling
authors: JL
authors: Wang
authors: Department of Otolaryngol Head Neck Surgery, General Hospital of Tianjin Medical University, Tianjin, China.
authors: Xin
authors: X
authors: Wang
authors: Department of Otolaryngol Head Neck Surgery, General Hospital of Tianjin Medical University, Tianjin, China.
authors: Dong
authors: D
authors: Yang
authors: Department of Otolaryngol Head Neck Surgery, Tianjin First Central Hospital, Tianjin, China. dr_shiwenjie@hotmail.com.
authors: Wen Jie
authors: WJ
authors: Shi
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D000077195:Squamous Cell Carcinoma of Head and Neck; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: MiR-155; biomarker; diagnosis; laryngeal squamous cell carcinoma