title: Alterations in innate immunity reactants and carbohydrate and lipid metabolism precede occurrence of metritis in transition dairy cows.
abstract: The overall purpose of the present study was to search for early screening biomarkers of disease state. Therefore the objectives of this study were to evaluate metabolites related to carbohydrate metabolism, acute phase proteins, and proinflammatory cytokines in the blood of transition dairy cows starting at -8 weeks before calving. Blood samples were collected from 100 multiparous Holstein dairy cows during -8, -4, disease diagnosis, +4 and +8 weeks relative to parturition. Six healthy cows and 6 cows that showed clinical signs of metritis were selected for serum analysis. Overall the results showed that cows with metritis had greater concentration of lactate, interleukin-6 (IL-6), tumor necrosis factor (TNF), and serum amyloid A (SAA) versus healthy cows throughout the experiment. The disease was associated with decrease in milk production and fat: protein ratio. Cows with metritis showed alteration in metabolites related to carbohydrate metabolism, acute phase proteins, and proinflammatory cytokines starting at -8 weeks prior to parturition and appearance of clinical signs of the disease. This study suggests a possible use of cytokines as early markers of disease in dairy cows.
authors: Department of Agricultural Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
authors: Elda
authors: E
authors: Dervishi
authors: Department of Agricultural Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
authors: Guanshi
authors: G
authors: Zhang
authors: Department of Agricultural Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
authors: Dagnachew
authors: D
authors: Hailemariam
authors: Department of Agricultural Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
authors: Seyed Ali
authors: SA
authors: Goldansaz
authors: Department of Agricultural Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
authors: Qilan
authors: Q
authors: Deng
authors: Department of Agricultural Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
authors: Suzanna M
authors: SM
authors: Dunn
authors: Department of Agricultural Food, and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada. Electronic address: burim.ametaj@ualberta.ca.
authors: Burim N
authors: BN
authors: Ametaj
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D050260:Carbohydrate Metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D016207:Cytokines / Q000097:blood*; D005260:Female; D007113:Immunity, Innate; D007249:Inflammation / Q000276:immunology / Q000662:veterinary*; D036801:Parturition; D049590:Postpartum Period / Q000276:immunology; D014591:Uterine Diseases / Q000276:immunology / Q000662:veterinary*
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; D016207:Cytokines
keywords: Acute phase protein; Blood metabolites; Cytokines; Dairy cows; Metritis