title: Effect of feed starvation on side-stream anammox activity and key microbial populations.
abstract: The anaerobic ammonium oxidation (anammox) process is widely acknowledged to be susceptible to a wide range of environmental factors given the slow growth rate of the anammox bacteria. Surprisingly there is limited experimental data regarding the susceptibility of the anammox process to feed starvations which may be encountered in full-scale applications. Therefore, a study was established to investigate the impact of feed starvations on nitritation and anammox activity in a demonstration-scale sequencing batch reactor. Three starvation periods were trialled, lasting one fortnight (15 d), one month (33 d) and two months (62 d). Regardless of the duration of the starvation period, assessment of the ammonia removal performance demonstrated nitritation and anammox activity were reinstated within one day of recovery operation. Characterisation of the community structure using 16S rRNA and functional genes specific for nitrogen-related microbes showed there was no clear impact or shift in the microbial populations between starvation and recovery phases.
authors: South Australian Water Corporation, Adelaide, 5000, South Australia, Australia. Electronic address: Petra.Reeve@sawater.com.au.
authors: Petra J
authors: PJ
authors: Reeve
authors: Allwater JV, Adelaide, 5000, South Australia, Australia.
authors: Irina
authors: I
authors: Mouilleron
authors: Department of Environmental Engineering and Sustainable Environment Research Centre, National Cheng Kung University, Tainan, 70701, Taiwan.
authors: Hui-Ping
authors: HP
authors: Chuang
authors: South Australian Water Corporation, Adelaide, 5000, South Australia, Australia.
authors: Ben
authors: B
authors: Thwaites
authors: Allwater JV, Adelaide, 5000, South Australia, Australia.
authors: Kylie
authors: K
authors: Hyde
authors: South Australian Water Corporation, Adelaide, 5000, South Australia, Australia.
authors: Nirmala
authors: N
authors: Dinesh
authors: Institute for Water Quality, Resource and Waste Management, Vienna University of Technology, Karlsplatz, 1040, Vienna, Austria.
authors: Joerg
authors: J
authors: Krampe
authors: Department of Environmental Engineering and Sustainable Environment Research Centre, National Cheng Kung University, Tainan, 70701, Taiwan.
authors: Tsair-Fuh
authors: TF
authors: Lin
authors: South Australian Water Corporation, Adelaide, 5000, South Australia, Australia; Health and Environment Group, School of the Environment, Flinders University, Bedford Park, 5042, South Australia, Australia; Centre for Water Management and Reuse, School of Natural and Built Environments, University of South Australia, Mawson Lakes, 5095, South Australia, Australia.
authors: Ben
authors: B
authors: van den Akker
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D005798:Genes, Bacterial; D009584:Nitrogen / Q000032:analysis*; D010084:Oxidation-Reduction; D010865:Pilot Projects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods*
chemical_list: D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D000641:Ammonia; D009584:Nitrogen
keywords: Anaerobic ammonium oxidation; Microbial populations; Nitritation; Starvation