title: Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats.
abstract: OBJECTIVE
  To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model.
  
  
  METHODS
  Ten female Wistar rats were divided randomly in two groups. Group A - ADR; Group B - FA+ADR. Dams from group B received daily, since two weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams from both groups received ADR (6mk/kg) by intraperitoneal injection on gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 and were examined looking for ARMs. The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p≤0.05*.
  
  
  RESULTS
  81 fetuses were harvested. The number of fetuses; number of ARMs; mean (∆%) (± SD) were determined to be, respectively: ADR - 41[29;65%(±37%)] versus FA+ADR - 40[04;16%(±36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in ADR group (p=0.05). The thickness (µm) of ASSE (± SD) and IE (± SD) were measured, respectively: ADR - [25.98(±0.74) and 19.48(±1.68)] versus FA+ADR - [24.74(±0.91) and 24.80(±0.81)] (p<0.005). The thickness of IE was significantly enlarged when FA was given (p<0.005).
  
  
  CONCLUSIONS
  Single dose of adriamycin on D8 was able to induce anorectal malformations. Folic acid reduces the number and enlarged the IE of ARMs ADR-induced.
authors: Universidade Federal de São Paulo, Brazil.
authors: Danilo José Fiorindo
authors: DJ
authors: Faria
authors: Department of Morphology, UNIFESP, Sao Paulo, SP, Brazil.
authors: Manuel de Jesus
authors: Mde J
authors: Simões
authors: UNIFESP, Sao Paulo, SP, Brazil.
authors: Luciana Cristina
authors: LC
authors: Teixeira
authors: Universidade São Judas Tadeu, Sao Paulo, SP, Brazil.
authors: Angela Tucci Pinheiro
authors: AT
authors: Faria
authors: UNIFESP, Sao Paulo, SP, Brazil.
authors: Álvaro Edmundo Simões Ulhoa
authors: ÁE
authors: Cintra
authors: Department of Pediatric Surgery, UNIFESP, Sao Paulo, SP, Brazil.
authors: José Luiz
authors: JL
authors: Martins
mesh_terms: D000818:Animals; D000071056:Anorectal Malformations; D001006:Anus, Imperforate / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004317:Doxorubicin; D004848:Epithelium / Q000002:abnormalities / Q000473:pathology; D005260:Female; D005333:Fetus / Q000002:abnormalities; D005492:Folic Acid / Q000008:administration & dosage*; D011247:Pregnancy; D011897:Random Allocation; D017208:Rats, Wistar; D059005:Topoisomerase II Inhibitors
chemical_list: D059005:Topoisomerase II Inhibitors; D004317:Doxorubicin; D005492:Folic Acid