title: Proteomic data on the nuclear interactome of human MCM9.
abstract: We present data relating to the interactome of MCM9 from the nuclei of human cells. MCM9 belongs to the AAA+ superfamily, and contains an MCM domain and motifs that may confer DNA helicase activity. MCM9 has been shown to bind MCM8, and has been implicated in DNA replication and homologous recombination. However, the mechanistic basis of MCM9's role in DNA repair is poorly understood, and proteins with which it interacts were hitherto unknown. We performed tandem affinity purification of MCM9 and its interacting proteins from nuclear extracts of human cells, followed by proteomic analysis, thereby generating a set of mass spectrometry data corresponding to the MCM9 interactome [1]. The proteomic data set comprises 29 mass spectrometry RAW files, deposited to the ProteomeXchange Consortium, and freely available from the PRIDE partner repository with the data set identifier PXD000212. A set of 22 interacting proteins identified from the proteomic data was used to create an MCM9-centered interactive network diagram, using the Cytoscape program. These data allow the scientific community to access, mine and explore the human nuclear MCM9 interactome.
authors: Laboratory of DNA Replication and Genome Dynamics, Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors: James R A
authors: JR
authors: Hutchins
authors: Laboratory of DNA Replication and Genome Dynamics, Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors: Sabine
authors: S
authors: Traver
authors: Laboratory of DNA Replication and Genome Dynamics, Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors: Philippe
authors: P
authors: Coulombe
authors: Laboratory of DNA Replication and Genome Dynamics, Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors: Isabelle
authors: I
authors: Peiffer
authors: Laboratory of DNA Replication and Genome Dynamics, Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors: Magali
authors: M
authors: Kitzmann
authors: Laboratory of Gene Regulation, Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors: Daniel
authors: D
authors: Latreille
authors: Laboratory of DNA Replication and Genome Dynamics, Institute of Human Genetics, CNRS, 141 rue de la Cardonille, 34396 Montpellier, France.
authors: Marcel
authors: M
authors: Méchali
keywords: Affinity Purification; DNA Replication And Repair; Mass Spectrometry; Protein-Protein Interactions; Proteomics Data