title: Effect of miR-143-3p on C2C12 myoblast differentiation.
abstract: MicroRNAs are a class of 18-22 nucleotide non-coding RNAs that modulate gene expression by associating with the 3' untranslated regions of mRNAs. A large number of microRNAs are involved in the regulation of myoblast differentiation, many of which remain undiscovered. In this study, we found that miR-143-3p was upregulated during C2C12 myoblast differentiation and over-expression of miR-143-3p significantly inhibited the relative expression levels of MyoD, MyoG, myf5, and MyHC genes, especially in the later stages of differentiation. In addition, miR-143-3p inhibited expression of genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicate that miR-143-3p represents a new myogenic differentiation-associated microRNA that can inhibit C2C12 myoblast differentiation, especially in the later stages of differentiation.
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Jingjing
authors: J
authors: Du
authors: b Department of Animal Science , Xichang College , Xichang , China.
authors: Yi
authors: Y
authors: Zhang
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Linyuan
authors: L
authors: Shen
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Jia
authors: J
authors: Luo
authors: c Department of Agricultural, Food and Nutritional Science , University of Alberta , Edmonton , Canada.
authors: Huaigang
authors: H
authors: Lei
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Peiwen
authors: P
authors: Zhang
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Qiang
authors: Q
authors: Pu
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Yihui
authors: Y
authors: Liu
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Surong
authors: S
authors: Shuai
authors: d Sichuan Provincial Agricultural Department, Sichuan Province General Station of Animal Husbandry , Chengdu , China.
authors: Qiang
authors: Q
authors: Li
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Xuewi
authors: X
authors: Li
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Shunhua
authors: S
authors: Zhang
authors: a College of Animal Science and Technology , Sichuan Agricultural University , Chengdu , China.
authors: Li
authors: L
authors: Zhu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology*
chemical_list: D035683:MicroRNAs; C532884:MIRN143 microRNA, mouse
keywords: C2C12; Wnt pathway; miR-143-3p; myoblast differentiation