title: Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy.
abstract: Adhesion of cancer cells with different metastatic potential and anticancer drug resistance has been quantitatively evaluated by using self-assembled monolayer (SAM)-patterned substrates and reflection interference contrast microscopy (RICM). Cell-adhesive SAM spots with optimized diameter could prevent cell-cell adhesion and thus allowed the systematic evaluation of statistically reliable numbers of contact area between single cancer cells and substrates by RICM. The statistical image analysis revealed that highly metastatic mouse melanoma cells showed larger contact area than lowly metastatic cells. We also found that both cancer cell types exhibited distinct transition from the "strong" to "weak" adhesion states with increase in the concentration of (-)-epigallocatechin gallate (EGCG), which is known to exhibit cancer preventive activity. Mathematical analysis of the adhesion transition revealed that adhesion of the highly metastatic mouse melanoma cells showed more EGCG tolerance than that of lowly metastatic cells. Moreover, time-lapse RICM observation revealed that EGCG weakened cancer cell adhesion in a stepwise manner, probably via focal adhesion complex. These results clearly indicate that contact area can be used as a quantitative measure for the determination of cancer phenotypes and their drug resistance, which will provide physical insights into the mechanism of cancer metastasis and cancer prevention.
authors: Takahisa
authors: T
authors: Matsuzaki
authors: Kosaku
authors: K
authors: Ito
authors: School of Science and Engineering, Waseda University , Okubo 3-4-1, Shinjuku, Tokyo 169-855, Japan.
authors: Kentaro
authors: K
authors: Masuda
authors: School of Science and Engineering, Waseda University , Okubo 3-4-1, Shinjuku, Tokyo 169-855, Japan.
authors: Eisuke
authors: E
authors: Kakinuma
authors: School of Science and Engineering, Waseda University , Okubo 3-4-1, Shinjuku, Tokyo 169-855, Japan.
authors: Rumi
authors: R
authors: Sakamoto
authors: Kentaro
authors: K
authors: Iketaki
authors: Frontier Research Institute for Interdisciplinary Sciences, Tohoku University , 6-3 Aramakiazaaoba, Aoba, Sendai, Miyagi 980-8578, Japan.
authors: Hideaki
authors: H
authors: Yamamoto
authors: Masami
authors: M
authors: Suganuma
authors: Naritaka
authors: N
authors: Kobayashi
authors: Seiichiro
authors: S
authors: Nakabayashi
authors: School of Science and Engineering, Waseda University , Okubo 3-4-1, Shinjuku, Tokyo 169-855, Japan.
authors: Takashi
authors: T
authors: Tanii
authors: Hiroshi Y
authors: HY
authors: Yoshikawa
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D006801:Humans; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008857:Microscopy, Interference / Q000379:methods*; D009362:Neoplasm Metastasis / Q000188:drug therapy / Q000473:pathology; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology*
chemical_list: D016588:Anticarcinogenic Agents; D002392:Catechin; C045651:epigallocatechin gallate