title: Study of the Association of Mutant HBsAg Gene and Hodgkin and Non-Hodgkin Lymphoma.
abstract: BACKGROUND
  Hepatitis B Virus (HBV) is responsible for chronic, acute, and fulminant hepatitis, which are prevalent worldwide. Chronic HBV may lead to cirrhosis and hepatocellular carcinoma. Several epidemiological studies have indicated that hepatitis B virus is involved in B-cell Hodgkin and Non-Hodgkin Lymphoma (NHL).
  
  
  OBJECTIVE
  The aim of this study was to evaluate the association between hepatitis B infection and Hodgkin and non-Hodgkin Lymphoma.
  
  
  METHODS
  Paraffin embedded of 41 block samples including 12 (29.26%) Hodgkin and 29 (70.73%) non-Hodgkin patients were collected. Next, DNA extraction was carried out for all the samples followed by HBV DNA detection by the nested polymerase chain reaction (PCR). The positive HBV DNA samples were sequenced, and HBV genotypes and HBV subtypes were determined.
  
  
  RESULTS
  Three out of 12 (25%) Hodgkin samples and seven out of 29 (24.13%) non-Hodgkin showed positive HBV DNA results. The results of sequencing revealed that the D genotype was predominant among the positive HBV patients. Interestingly an unpredictable amino acid proline was detected in position 88 of the HBs gene, which indicates a new mutation in the "S" region of HBV DNA in patients with Hodgkin and non-Hodgkin lymphoma.
  
  
  CONCLUSIONS
  A high rate of 25% and 24.13% of HBV DNA was detected among patients with Hodgkin and non-Hodgkin lymphoma, respectively.
authors: Infectious and Tropical Disease Research Center, Health Research Institute, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Kamyar
authors: K
authors: Makvandi
authors: Department of Pathology, Imam Khomeini hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Nastaran
authors: N
authors: Ranjbari
authors: Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Manoochehr
authors: M
authors: Makvandi
authors: Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Ali
authors: A
authors: Ashraf Teimori
authors: Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Niloofar
authors: N
authors: Neisi
authors: Infectious and Tropical Disease Research Center, Health Research Institute, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Mojtaba
authors: M
authors: Rasti
authors: Infectious and Tropical Disease Research Center, Health Research Institute, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Vida
authors: V
authors: Alipour
authors: Infectious and Tropical Disease Research Center, Health Research Institute, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Mostafa
authors: M
authors: Albokord
authors: Department of Pathology, Imam Khomeini hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Malek
authors: M
authors: Kanani
authors: Department of Nuclear Medicine, Golestan Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Ramezan
authors: R
authors: Ahadi
authors: Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Ala
authors: A
authors: Habibian
keywords: Hepatitis B Virus; Hodgkin Disease; Polymerase Chain Reaction