title: Loss of miR-182 affects B-cell extrafollicular antibody response.
abstract: MicroRNAs have been shown to play a role in B-cell differentiation and activation. Here, we found miR-182 to be highly induced in activated B cells. However, mice lacking miR-182 have normal B-cell and T-cell development. Interestingly, mutant mice exhibited a defective antibody response at early time-points in the immunization regimen when challenged with a T-cell-dependent antigen. Germinal centres were formed but the generation of extrafollicular plasma cells was defective in the spleens of immunized miR-182-deficient mice. Mutant mice were also not able to respond to a T-cell-independent type 2 antigen, which typically elicited an extrafollicular B-cell response. Taken together, the data indicated that miR-182 plays a critical role in driving extrafollicular B-cell antibody responses.
authors: Immunology Group, Bioprocessing Technology Institute, Agency for Science Technology and Research, Singapore, Singapore.
authors: Yan-Feng
authors: YF
authors: Li
authors: Immunology Group, Bioprocessing Technology Institute, Agency for Science Technology and Research, Singapore, Singapore.
authors: Xijun
authors: X
authors: Ou
authors: Immunology Group, Bioprocessing Technology Institute, Agency for Science Technology and Research, Singapore, Singapore.|Department of Physiology, National University of Singapore, Singapore, Singapore.
authors: Shengli
authors: S
authors: Xu
authors: Lab for Stem Cell & Retinal Regeneration, Division of Ophthalmic Genetics, The Eye Hospital of Wenzhou Medical University, Suita, Japan.
authors: Zi-Bing
authors: ZB
authors: Jin
authors: Department of Epidemiology, National Cardiovascular Centre, Suita, Japan.
authors: Naoharu
authors: N
authors: Iwai
authors: Immunology Group, Bioprocessing Technology Institute, Agency for Science Technology and Research, Singapore, Singapore.|Department of Physiology, National University of Singapore, Singapore, Singapore.|Department of Microbiology, National University of Singapore, Singapore, Singapore.|Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.|School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
authors: Kong-Peng
authors: KP
authors: Lam
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000235:genetics; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D018858:Germinal Center / Q000502:physiology*; D007114:Immunization; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D010950:Plasma Cells / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D035683:MicroRNAs; C540524:Mirn182 microRNA, mouse
keywords: antibody response; extrafollicular B cells; germinal centres; miR-182