title: Lidocaine Concentration in Oral Tissue by the Addition of Epinephrine.
abstract: The vasoconstrictive effect due to the addition of epinephrine to local anesthetic has been clearly shown by measuring blood-flow volume or blood anesthetic concentration in oral mucosal tissue. However, there are no reports on the measurement of anesthetic concentration using samples directly taken from the jawbone and oral mucosal tissue. Consequently, in this study, the effect of lidocaine concentration in the jawbone and oral mucosal tissue by the addition of epinephrine to the local anesthetic lidocaine was considered by quantitatively measuring lidocaine concentration within the tissue. Japanese white male rabbits (n = 96) were used as test animals. General anesthesia was induced by sevoflurane and oxygen, and then cannulation to the femoral artery was performed while arterial pressure was constantly recorded. Infiltration anesthesia was achieved by 0.5 mL of 2% lidocaine containing 1 : 80,000 epinephrine in the upper jawbone (E(+)) and 0.5 mL of 2% of epinephrine additive-free lidocaine (E(0)) under the periosteum. At specified time increments (10, 20, 30, 40, 50, and 60 minutes), samples from the jawbone, oral mucosa, and blood were collected, and lidocaine concentration was directly measured by high-performance liquid chromatography. No significant differences in the change in blood pressure were observed either in E(+) or E(0). In both E(+) and E(0) groups, the serum lidocaine concentration peaked 10 minutes after local anesthesia and decreased thereafter. At all time increments, serum lidocaine concentration in E(+) was significantly lower than that in E(0). There were no significant differences in measured lidocaine concentration between jawbone and mucosa within either the E(+) or the E(0) groups at all time points, although the E(0) group had significantly lower jawbone and mucosa concentrations than the E(+) group at all time points when comparing the 2 groups to each other. Addition of epinephrine to the local anesthetic inhibited systemic absorption of local anesthetic into the blood such that a high concentration could be maintained in the tissue. Epinephrine-induced vasoconstrictive effect was observed not only in the oral mucosa but also in the jawbone.
authors: Postgraduate Student (Dentist).
authors: Eri
authors: E
authors: Tanaka
authors: Postgraduate Student (Dentist).
authors: Kenji
authors: K
authors: Yoshida
authors: Associate Professor, and.
authors: Hiroyoshi
authors: H
authors: Kawaai
authors: Professor, Department of Dental Anesthesiology, School of Dentistry, Ohu University, Fukushima, Japan.
authors: Shinya
authors: S
authors: Yamazaki
mesh_terms: D065567:Absorption, Physiological / Q000187:drug effects; D000779:Anesthetics, Local / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D000818:Animals; D062186:Arterial Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004837:Epinephrine / Q000008:administration & dosage* / Q000097:blood; D008012:Lidocaine / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D008297:Male; D008437:Maxilla / Q000737:chemistry*; D009061:Mouth Mucosa / Q000737:chemistry*; D011817:Rabbits; D013997:Time Factors; D014018:Tissue Distribution / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000008:administration & dosage* / Q000097:blood
chemical_list: D000779:Anesthetics, Local; D014662:Vasoconstrictor Agents; D008012:Lidocaine; D004837:Epinephrine
keywords: Epinephrine; Infiltration anesthesia; Jawbone; Lidocaine concentration; Rabbit