title: Nanotubes-Embedded Indocyanine Green-Hyaluronic Acid Nanoparticles for Photoacoustic-Imaging-Guided Phototherapy.
abstract: Phototherapy is a light-triggered treatment for tumor ablation and growth inhibition via photodynamic therapy (PDT) and photothermal therapy (PTT). Despite extensive studies in this area, a major challenge is the lack of selective and effective phototherapy agents that can specifically accumulate in tumors to reach a therapeutic concentration. Although recent attempts have produced photosensitizers complexed with photothermal nanomaterials, the tedious preparation steps and poor tumor efficiency of therapy still hampers the broad utilization of these nanocarriers. Herein, we developed a CD44 targeted photoacoustic (PA) nanophototherapy agent by conjugating Indocyanine Green (ICG) to hyaluronic acid nanoparticles (HANPs) encapsulated with single-walled carbon nanotubes (SWCNTs), resulting in a theranostic nanocomplex of ICG-HANP/SWCNTs (IHANPT). We fully characterized its physical features as well as PA imaging and photothermal and photodynamic therapy properties in vitro and in vivo. Systemic delivery of IHANPT theranostic nanoparticles led to the accumulation of the targeted nanoparticles in tumors in a human cancer xenograft model in nude mice. PA imaging confirmed targeted delivery of the IHANPT nanoparticles into tumors (T/M ratio = 5.19 ± 0.3). The effect of phototherapy was demonstrated by low-power laser irradiation (808 nm, 0.8 W/cm(2)) to induce efficient photodynamic effect from ICG dye. The photothermal effect from the ICG and SWCNTs rapidly raised the tumor temperature to 55.4 ± 1.8 °C. As the result, significant tumor growth inhibition and marked induction of tumor cell death and necrosis were observed in the tumors in the tumors. There were no apparent systemic and local toxic effects found in the mice. The dynamic thermal stability of IHANPT was studied to ensure that PTT does not affect ICG-dependent PDT in phototherapy. Therefore, our results highlight imaging property and therapeutic effect of the novel IHANPT theranostic nanoparticle for CD44 targeted and PA image-guided dual PTT and PDT cancer therapy.
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University , Xiamen, Fujian 361005, China.
authors: Guohao
authors: G
authors: Wang
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University , Xiamen, Fujian 361005, China.
authors: Fan
authors: F
authors: Zhang
authors: Department of Ophthalmology Second Hospital, Jilin University , Changchun, Jilin 130033, China.
authors: Rui
authors: R
authors: Tian
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University , Xiamen, Fujian 361005, China.
authors: Liwen
authors: L
authors: Zhang
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University , Xiamen, Fujian 361005, China.
authors: Guifeng
authors: G
authors: Fu
authors: Departments of Surgery and Radiology and Imaging Sciences, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
authors: Lily
authors: L
authors: Yang
authors: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University , Xiamen, Fujian 361005, China.|Departments of Surgery and Radiology and Imaging Sciences, Emory University School of Medicine , Atlanta, Georgia 30322, United States.
authors: Lei
authors: L
authors: Zhu
mesh_terms: D000818:Animals; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry / Q000627:therapeutic use; D007208:Indocyanine Green / Q000737:chemistry / Q000627:therapeutic use; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D061088:Photoacoustic Techniques; D010789:Phototherapy / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
chemical_list: D018960:Hyaluronan Receptors; D037742:Nanotubes, Carbon; D006820:Hyaluronic Acid; D007208:Indocyanine Green
keywords: hyaluronic acid; indocyanine green; photodynamic therapy; photothermal therapy; single wall carbon nanotube