title: Comparison of Central Corneal Thickness Between Fourier-Domain OCT, Very High-Frequency Digital Ultrasound, and Scheimpflug Imaging Systems.
abstract: OBJECTIVE
  To compare corneal thickness measurements between three imaging systems.
  
  
  METHODS
  In this retrospective study of 81 virgin and 58 post-laser refractive surgery corneas, central and minimum corneal thickness were measured using optical coherence tomography (OCT), very high-frequency digital ultrasound (VHF digital ultrasound), and a Scheimpflug imaging system. Agreement between methods was analyzed using mean differences (bias) (OCT - VHF digital ultrasound, OCT - Scheimpflug, VHF digital ultrasound - Scheimpflug) and Bland-Altman analysis with 95% limits of agreement (LoA).
  
  
  RESULTS
  Virgin cornea mean central corneal thickness was 508.3 ± 33.2 µm (range: 434 to 588 µm) for OCT, 512.7 ± 32.2 µm (range: 440 to 587 µm) for VHF digital ultrasound, and 530.2 ± 32.6 µm (range: 463 to 612 µm) for Scheimpflug imaging. OCT and VHF digital ultrasound showed the closest agreement with a bias of -4.37 µm, 95% LoA ±12.6 µm. Least agreement was between OCT and Scheimpflug imaging with a bias of -21.9 µm, 95% LoA ±20.7 µm. Bias between VHF digital ultrasound and Scheimpflug imaging was -17.5 µm, 95% LoA ±19.0 µm. In post-laser refractive surgery corneas, mean central corneal thickness was 417.9 ± 47.1 µm (range: 342 to 557 µm) for OCT, 426.3 ± 47.1 µm (range: 363 to 563 µm) for VHF digital ultrasound, and 437.0 ± 48.5 µm (range: 359 to 571 µm) for Scheimpflug imaging. Closest agreement was between OCT and VHF digital ultrasound with a bias of -8.45 µm, 95% LoA ±13.2 µm. Least agreement was between OCT and Scheimpflug imaging with a bias of -19.2 µm, 95% LoA ±19.2 µm. Bias between VHF digital ultrasound and Scheimpflug imaging was -10.7 µm, 95% LoA ±20.0 µm. No relationship was observed between difference in central corneal thickness measurements and mean central corneal thickness. Results were similar for minimum corneal thickness.
  
  
  CONCLUSIONS
  Central and minimum corneal thickness was measured thinnest by OCT and thickest by Scheimpflug imaging in both groups. A clinically significant bias existed between Scheimpflug imaging and the other two modalities.
authors: Timothy E
authors: TE
authors: Yap
authors: Timothy J
authors: TJ
authors: Archer
authors: Marine
authors: M
authors: Gobbe
authors: Dan Z
authors: DZ
authors: Reinstein
mesh_terms: D000328:Adult; D000368:Aged; D003315:Cornea / Q000033:anatomy & histology / Q000473:pathology*; D048988:Corneal Surgery, Laser; D003952:Diagnostic Imaging / Q000379:methods*; D003941:Diagnostic Techniques, Ophthalmological / Q000295:instrumentation*; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009929:Organ Size; D015203:Reproducibility of Results; D012189:Retrospective Studies; D041623:Tomography, Optical Coherence / Q000379:methods; D014465:Ultrasonics / Q000379:methods; D055815:Young Adult