title: A novel strategy for the determination of polycyclic aromatic hydrocarbon monohydroxylated metabolites in urine using ultra-high-performance liquid chromatography with tandem mass spectrometry.
abstract: In this study, a novel analytical approach for the determination of 11 monohydroxylated polycyclic aromatic hydrocarbon metabolites (OH-PAHs) in urine was developed and validated. The rapid, simple and high-throughput sample preparation procedure based on ethyl acetate extraction and subsequent purification by dispersive solid-phase extraction (d-SPE) employing a Z-Sep sorbent is used for the first time. For the identification/quantification of target compounds, ultra-high-performance liquid chromatography (U-HPLC) interfaced with tandem mass spectrometry (MS/MS) was applied. The results of validation experiments performed on the Standard Reference Material (SRM) 3673 (organic contaminants in non-smokers' urine) were in accordance with the certified values. The method recoveries ranged from 77 to 114 % with the relative standard deviation lower than 20 % and the quantification limits in the range of 0.010-0.025 ng mL(-1) (except for benzo[a]pyren-3-ol with 0.9 ng mL(-1)). Within the pilot study, the new method was used for the analysis of OH-PAHs in 50 urine samples. The concentrations of ΣOH-PAHs were in the range of 0.87-63 ng mL(-1) (1600-33,000 ng g(-1) creatinine), with naphthalen-2-ol (2-OH-NAP) and phenanthren-1-ol (1-OH-PHEN) being the most abundant exposure biomarkers detected in all samples.
authors: Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague 6, Czech Republic.
authors: Darina
authors: D
authors: Lankova
authors: Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague 6, Czech Republic.
authors: Katerina
authors: K
authors: Urbancova
authors: Institute of Experimental Medicine AS CR, Videnska 1083, 142 20, Prague 4, Czech Republic.
authors: Radim J
authors: RJ
authors: Sram
authors: Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague 6, Czech Republic.
authors: Jana
authors: J
authors: Hajslova
authors: Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Technicka 3, 166 28, Prague 6, Czech Republic. jana.pulkrabova@vscht.cz.
authors: Jana
authors: J
authors: Pulkrabova
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006900:Hydroxylation; D011084:Polycyclic Aromatic Hydrocarbons / Q000652:urine*; D012015:Reference Standards; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons
keywords: Monohydroxylated metabolites of polycyclic aromatic hydrocarbons; SRM 3673; Tandem mass spectrometry; Ultra-high-performance liquid chromatography; Urine