title: Expression of fibronectin binding protein A (FnBPA) from Staphylococcus aureus at the cell surface of Lactococcus lactis improves its immunomodulatory properties when used as protein delivery vector.
abstract: A recombinant strain of Lactococcus lactis displaying a cell-surface anchored fibronectin binding protein A (FnBPA) from Staphylococcus aureus (LL-FnBPA) had been shown to be more efficient in delivering plasmid than its wild-type counterpart both in vitro and in vivo, and have the ability to orientate the immune response toward a Th2 profile in a context of a DNA vaccination. The aim of this work was to test whether this LL-FnBPA strain could shape the immune response after mucosal administration in mice. For this, we used a mouse model of human papilloma virus (HPV)-induced cancer and a L. lactis strain displaying at its cell surface both HPV-16-E7 antigen (LL-E7) and FnBPA (LL-E7+FnBPA). Our results revealed a more efficient systemic Th1 immune response with recombinant LL-E7+FnBPA. Furthermore, mice vaccinated with LL-E7+FnBPA were better protected when challenged with HPV-16-induced tumors. Altogether, the results suggest that FnBPA displays adjuvant properties when used in the context of mucosal delivery using L. lactis as a live vector.
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France; UFMG, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors: Juliana F
authors: JF
authors: Almeida
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France; UFMG, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors: Natalia M
authors: NM
authors: Breyner
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France; Laboratory of Food Microbiology, Biology Department, University of Nature, Life and Hearth Sciences, Theniat el Had Street, 44225 Khemis Miliana, Algeria.
authors: Miloud
authors: M
authors: Mahi
authors: Laboratory of Food Microbiology, Biology Department, University of Nature, Life and Hearth Sciences, Theniat el Had Street, 44225 Khemis Miliana, Algeria.
authors: Bensoltane
authors: B
authors: Ahmed
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France; Laboratory of Beneficial Microorganisms, Functional Foods and Health, Department of Biology, University of Mostaganem, Lahcen Street, P.O. Box 300, Mostaganem 27000, Algeria.
authors: Bouasria
authors: B
authors: Benbouziane
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France; UFMG, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors: Priscilla C B Vilas
authors: PC
authors: Boas
authors: UFMG, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors: Anderson
authors: A
authors: Miyoshi
authors: UFMG, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors: Vasco
authors: V
authors: Azevedo
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors: Philippe
authors: P
authors: Langella
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
authors: Luis G
authors: LG
authors: Bermúdez-Humarán
authors: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France. Electronic address: jean-marc.chatel@jouy.inra.fr.
authors: Jean-Marc
authors: JM
authors: Chatel
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D018938:Caco-2 Cells; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D061505:Cell Surface Display Techniques; D005260:Female; D052162:Human papillomavirus 16; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D013294:Lactococcus lactis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050725:Papillomavirus E7 Proteins / Q000276:immunology*; D030361:Papillomavirus Infections / Q000517:prevention & control*; D010957:Plasmids; D013211:Staphylococcus aureus; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D019496:Cancer Vaccines; D050725:Papillomavirus E7 Proteins; C078635:fibronectin-binding proteins, bacterial; C059731:oncogene protein E7, Human papillomavirus type 16
keywords: Fibronectin binding protein A (FnBPA); HPV-16-E7 antigen; Human papillomavirus (HPV); Lactic acid bacteria; Protein vaccination