title: Ochratoxin A and T-2 Toxin Induce Clonogenicity and Cell Migration in Human Colon Carcinoma and Fetal Lung Fibroblast Cell Lines.
abstract: T-2 toxin and Ochratoxin A (OTA) are toxic secondary metabolites produced by various fungi, and together they contaminate feedstuffs worldwide. T-2 toxin and OTA may exert carcinogenic action in rodent. Despite the various in vivo experiments, carcinogenicity of these two mycotoxins has not yet been proven for human. In this current study, we proposed to investigate, in Human colon carcinoma cells and fetal lung fibroblast-like cells transfected with MYC, the effect of T-2 toxin and OTA on cell clonogenicity and cell migration. Results of the present investigation showed that T2-toxin as well as OTA has an important clonogenic effect in all cell lines, suggesting that these mycotoxins could promote the transcription of c-myc gene. Furthermore, T-2 toxin and OTA enhanced the migration effect of HCT116 cells at very low concentrations, proposing that these mycotoxins may exhibit carcinogenesis-like properties in the studied cells.
authors: Laboratoire de Recherche sur les Substances Biologiquement Compatibles (LRSBC), Faculté de Médecine Dentaire, 5019, Monastir, Tunisia.
authors: Haila
authors: H
authors: Abassi
authors: Laboratoire de Recherche sur les Substances Biologiquement Compatibles (LRSBC), Faculté de Médecine Dentaire, 5019, Monastir, Tunisia.
authors: Imen
authors: I
authors: Ayed-Boussema
authors: INSERM, U866, Université de Bourgogne, Dijon, F-21000, France.
authors: Sarah
authors: S
authors: Shirley
authors: Laboratoire de Recherche sur les Substances Biologiquement Compatibles (LRSBC), Faculté de Médecine Dentaire, 5019, Monastir, Tunisia.
authors: Salwa
authors: S
authors: Abid
authors: Laboratoire de Recherche sur les Substances Biologiquement Compatibles (LRSBC), Faculté de Médecine Dentaire, 5019, Monastir, Tunisia. hassen.bacha@fmdm.rnu.tn.
authors: Hassen
authors: H
authors: Bacha
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D009183:Mycotoxins / Q000633:toxicity*; D009793:Ochratoxins / Q000633:toxicity*; D013605:T-2 Toxin / Q000633:toxicity*; D014945:Wound Healing
chemical_list: D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D016604:Aflatoxin B1; D013605:T-2 Toxin
keywords: Aflatoxin B1; Cell Migration; Clonogenicity; Ochratoxin A; T2-Toxin