title: Novel arginine deiminase-based method to assay L-arginine in beverages.
abstract: A highly selective and sensitive enzymatic method for the quantitative determination of L-arginine (Arg) has been developed. The method is based on the use of recombinant bacterial arginine deiminase (ADI) isolated from the cells of a recombinant strain Escherichia coli and o-phthalaldehyde (OPA) as a chemical reagent. Ammonia, the product of the enzymatic digestion of Arg by ADI, reacts with OPA and forms in the presence of sulfite a product, which can be detected by spectrophotometry (S) and fluorometry (F). The linear concentration range for Arg assay in the final reaction mixture varies for ADI-OPA-F variant of the method from 0.35 μM to 24 μM with the detection limit of 0.25 μM. For ADI-OPA-S variant of the assay, the linearity varies from 0.7 μM to 50 μM with the detection limit of 0.55 μM. The new method was tested on real samples of wines and juices. A high correlation (R=0.978) was shown for the results obtained with the proposed and the reference enzymatic method.
authors: Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005 Lviv, Ukraine.
authors: N Ye
authors: NY
authors: Stasyuk
authors: Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005 Lviv, Ukraine. Electronic address: galina.gayda@gmail.com.
authors: G Z
authors: GZ
authors: Gayda
authors: Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005 Lviv, Ukraine.
authors: L R
authors: LR
authors: Fayura
authors: Department of Biochemistry and Hygiene, Lviv State University of Physical Culture, Kosciuszko Street 11, 79000 Lviv, Ukraine; Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005 Lviv, Ukraine.
authors: Y R
authors: YR
authors: Boretskyy
authors: Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005 Lviv, Ukraine; Institute of Applied Biotechnology and Basic Sciences, Rzeszow University, Sokolowska Str. 26, 36-100 Kolbuszowa, Poland.
authors: M V
authors: MV
authors: Gonchar
authors: Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Str. 14/16, 79005 Lviv, Ukraine; Department of Biotechnology and Microbiology, Rzeszow University, Cwiklinskiej 2, 35-601 Rzeszow, Poland.
authors: A A
authors: AA
authors: Sibirny
mesh_terms: D001120:Arginine / Q000737:chemistry*; D001628:Beverages; D001681:Biological Assay; D006867:Hydrolases / Q000737:chemistry*; D013053:Spectrophotometry; D014520:Urethane / Q000737:chemistry*
chemical_list: D014520:Urethane; D001120:Arginine; D006867:Hydrolases; C019723:arginine deiminase
keywords: Arginine deiminase; Beverages; Enzymatic assay; Ethyl carbamate; l-Arginine