title: Covalent Immobilization of Penicillin G Acylase onto Fe3O4@Chitosan Magnetic Nanoparticles.
abstract: Penicillin G acylase (PGA) was immobilized on magnetic Fe3O4@chitosan nanoparticles through the Schiff base reaction. The immobilization conditions were optimized as follows: enzyme/support 8.8 mg/g, pH 6.0, time 40 min, and temperature 25°C. Under these conditions, a high immobilization efficiency of 75% and a protein loading of 6.2 mg/g-support were obtained. Broader working pH and higher thermostability were achieved by the immobilization. In addition, the immobilized PGA retained 75% initial activity after ten cycles. Kinetic parameters Vmax and Km of the free and immobilized PGAs were determined as 0.91 mmol/min and 0.53 mmol/min, and 0.68 mM and 1.19 mM, respectively. Synthesis of amoxicillin with the immobilized PGA was carried out in 40% ethylene glycol at 25°C and a conversion of 72% was obtained. These results showed that the immobilization of PGA onto magnetic chitosan nanoparticles is an efficient and simple way for preparation of stable PGA.
authors: School of Pharmacy, Jiangsu University, Zhenjiang 212013, P.R. China.
authors: Xiao-Min
authors: XM
authors: Ling
authors: School of Pharmacy, Jiangsu University, Zhenjiang 212013, P.R. China.
authors: Xiang-Yu
authors: XY
authors: Wang
authors: School of Pharmacy, Jiangsu University, Zhenjiang 212013, P.R. China.
authors: Ping
authors: P
authors: Ma
authors: School of Pharmacy, Jiangsu University, Zhenjiang 212013, P.R. China.
authors: Yi
authors: Y
authors: Yang
authors: School of Pharmacy, Jiangsu University, Zhenjiang 212013, P.R. China.
authors: Jie-Mei
authors: JM
authors: Qin
authors: United Pharmaceutical Institute of Jiangsu University and Shandong Tianzhilvye Biotechnology Co. Ltd., Jiangsu University, Zhenjiang 212013, P.R. China.
authors: Xue-Jun
authors: XJ
authors: Zhang
authors: School of Pharmacy, Jiangsu University, Zhenjiang 212013, P.R. China.|United Pharmaceutical Institute of Jiangsu University and Shandong Tianzhilvye Biotechnology Co. Ltd., Jiangsu University, Zhenjiang 212013, P.R. China.
authors: Ye-Wang
authors: YW
authors: Zhang
mesh_terms: D000658:Amoxicillin / Q000138:chemical synthesis; D048271:Chitosan / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D019855:Ethylene Glycol / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D010399:Penicillin Amidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D000658:Amoxicillin; D048271:Chitosan; D010399:Penicillin Amidase; D019855:Ethylene Glycol
keywords: Amoxicillin; Chitosan; Immobilization; Magnetic nanoparticles; penicillin G acylase