title: Bacterial Bioburden Decrease in Orthokeratology Lens Storage Cases After Forewarning: Assessment by the DNA Dot Hybridization Assay.
abstract: BACKGROUND
  The aim of this study was to measure the changes in the bacterial bioburden in orthokeratology (OK) lens storage cases using the DNA dot hybridization assay (DHA) after forewarning patients about their bacterial contamination severity.
  
  
  METHODS
  Thirty-one OK lens wearers were prospectively enrolled in this study. Dot hybridization assay was used for serial measurements of bacterial bioburden in OK storage cases after lenses had been soaked for approximately 6 hr. After the first assessment, the lens wearers were informed of the extent of case contamination and the possible risk of microbial keratitis (MK), and best practices for lens care and lens case hygiene were reviewed and reinforced. A second assessment by the same DHA method was performed after approximately 6 months.
  
  
  RESULTS
  Two universal bacterial probes confirmed a significant decrease in bacterial bioburden at the second assessment (P<0.01 and P<0.001). Genus-specific probes showed significant reductions in Acinetobacter and Klebsiella (P=0.02 and P=0.01), but not in Pseudomonas (P=0.42).
  
  
  CONCLUSIONS
  Making OK lens wearers aware of the bacterial bioburden in their lens cases resulted in improved quality of case care and reduced bioburden. Our results suggest that a strategy of bioburden assessment with forewarning could be a useful method to decrease the incidence of OK-related MK.
authors: Department of Ophthalmology (P.-C.F., J.L., S.-L.T., Y.-H.L., M.-T.K.), Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan; Department of Medical Laboratory Science and Biotechnology (T.C.C.), College of Medicine, National Cheng Kung University, Tainan, Taiwan; Department of Laboratory Medicine (C.-C.C.), Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan; Graduate Institute of Clinical Medical Sciences (C.-C.H.), Chang Gung University and Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
authors: Po-Chiung
authors: PC
authors: Fang
authors: Jung
authors: J
authors: Lo
authors: Tsung C
authors: TC
authors: Chang
authors: Chun-Chih
authors: CC
authors: Chien
authors: Chang-Chun
authors: CC
authors: Hsiao
authors: Shin-Ling
authors: SL
authors: Tseng
authors: Yu-Hsuan
authors: YH
authors: Lai
authors: Ming-Tse
authors: MT
authors: Kuo
mesh_terms: D000293:Adolescent; D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification; D002648:Child; D018639:Contact Lens Solutions / Q000494:pharmacology; D003261:Contact Lenses / Q000382:microbiology*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004866:Equipment Contamination; D015818:Eye Infections, Bacterial / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016249:Optical Storage Devices; D054158:Orthokeratologic Procedures; D011446:Prospective Studies
chemical_list: D018639:Contact Lens Solutions; D015342:DNA Probes; D004269:DNA, Bacterial; D004247:DNA