title: Blended Polyurethane and Tropoelastin as a Novel Class of Biologically Interactive Elastomer.
abstract: Polyurethanes are versatile elastomers but suffer from biological limitations such as poor control over cell attachment and the associated disadvantages of increased fibrosis. We address this problem by presenting a novel strategy that retains elasticity while modulating biological performance. We describe a new biomaterial that comprises a blend of synthetic and natural elastomers: the biostable polyurethane Elast-Eon and the recombinant human tropoelastin protein. We demonstrate that the hybrid constructs yield a class of coblended elastomers with unique physical properties. Hybrid constructs displayed higher elasticity and linear stress-strain responses over more than threefold strain. The hybrid materials showed increased overall porosity and swelling in comparison to polyurethane alone, facilitating enhanced cellular interactions. In vitro, human dermal fibroblasts showed enhanced proliferation, while in vivo, following subcutaneous implantation in mice, hybrid scaffolds displayed a reduced fibrotic response and tunable degradation rate. To our knowledge, this is the first example of a blend of synthetic and natural elastomers and is a promising approach for generating tailored bioactive scaffolds for tissue repair.
authors: 1 The Heart Research Institute , Sydney, Australia .|2 Sydney Medical School, University of Sydney , Sydney, Australia .|3 School of Molecular Bioscience, University of Sydney , Sydney, Australia .
authors: Steven G
authors: SG
authors: Wise
authors: 2 Sydney Medical School, University of Sydney , Sydney, Australia .|4 Discipline of Pathology and School of Medical Science, University of Sydney , Sydney, Australia .|5 Charles Perkins Centre, University of Sydney , Sydney, Australia .|6 Bosch Institute, University of Sydney , Sydney, Australia .
authors: Hongjuan
authors: H
authors: Liu
authors: 3 School of Molecular Bioscience, University of Sydney , Sydney, Australia .|5 Charles Perkins Centre, University of Sydney , Sydney, Australia .
authors: Giselle C
authors: GC
authors: Yeo
authors: 1 The Heart Research Institute , Sydney, Australia .|2 Sydney Medical School, University of Sydney , Sydney, Australia .
authors: Praveesuda L
authors: PL
authors: Michael
authors: 1 The Heart Research Institute , Sydney, Australia .|2 Sydney Medical School, University of Sydney , Sydney, Australia .
authors: Alex H P
authors: AH
authors: Chan
authors: 3 School of Molecular Bioscience, University of Sydney , Sydney, Australia .
authors: Alan K Y
authors: AK
authors: Ngo
authors: 7 School of Physics, University of Sydney , Sydney, Australia .
authors: Marcela M M
authors: MM
authors: Bilek
authors: 2 Sydney Medical School, University of Sydney , Sydney, Australia .|4 Discipline of Pathology and School of Medical Science, University of Sydney , Sydney, Australia .|5 Charles Perkins Centre, University of Sydney , Sydney, Australia .|6 Bosch Institute, University of Sydney , Sydney, Australia .
authors: Shisan
authors: S
authors: Bao
authors: 3 School of Molecular Bioscience, University of Sydney , Sydney, Australia .|5 Charles Perkins Centre, University of Sydney , Sydney, Australia .|6 Bosch Institute, University of Sydney , Sydney, Australia .
authors: Anthony S
authors: AS
authors: Weiss
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004548:Elasticity / Q000187:drug effects; D020724:Elastomers / Q000494:pharmacology*; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D008810:Mice, Inbred C57BL; D011140:Polyurethanes / Q000494:pharmacology*; D016062:Porosity; D019919:Prosthesis Implantation; D012212:Rheology / Q000187:drug effects; D013314:Stress, Mechanical; D040521:Subcutaneous Tissue / Q000187:drug effects; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D014333:Tropoelastin / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D020724:Elastomers; D011140:Polyurethanes; D014333:Tropoelastin