title: An electrochemical biosensor to simultaneously detect VEGF and PSA for early prostate cancer diagnosis based on graphene oxide/ssDNA/PLLA nanoparticles.
abstract: Early diagnosis of prostate cancer (PCa) is critical for the prevention of metastasis and for early treatment; therefore, a simple and accurate device must be developed for this purpose. In this study, we reported a novel fabrication method for producing a dual-modality biosensor that can simultaneously detect vascular endothelial growth factor (VEGF) and prostate-specific antigen (PSA) in human serum for early diagnosis of PCa. This biosensor was constructed by coating graphene oxide/ssDNA (GO-ssDNA) on an Au-electrode for VEGF detection, and incorporated with poly-L-lactide nanoparticles (PLLA NPs) for signal amplification and PSA detection. The results showed that this biosensor has wide liner detection ranges (0.05-100ng/mL for VEGF and 1-100ng/mL for PSA), as well as high levels of sensitivity and selectivity (i.e., resisting interference from external factors, such as glucose, ascorbic acid human serum protein, immunoglobulin G, and immunoglobulin M), and demonstrated a high correlation with an enzyme-linked immunosorbent assay for sample detection in patients. Therefore, this biosensor could be utilized for early clinical diagnosis of PCa in the future.
authors: Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors: Lung-Hsuan
authors: LH
authors: Pan
authors: Department of Electrical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
authors: Shin-Hung
authors: SH
authors: Kuo
authors: Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors: Tzu-Yang
authors: TY
authors: Lin
authors: Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors: Chih-Wen
authors: CW
authors: Lin
authors: School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
authors: Po-Yu
authors: PY
authors: Fang
authors: Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. Electronic address: howardyang@mail.nsysu.edu.tw.
authors: Hung-Wei
authors: HW
authors: Yang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D004277:DNA, Single-Stranded / Q000737:chemistry*; D055088:Early Detection of Cancer; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D055910:Antibodies, Immobilized; D004277:DNA, Single-Stranded; D010087:Oxides; D011091:Polyesters; D042461:Vascular Endothelial Growth Factor A; C033616:poly(lactide); D006108:Graphite; D017430:Prostate-Specific Antigen
keywords: Electrochemical biosensor; Graphene oxide; Prostate cancer (PCa); Prostate-specific antigen (PSA); Single strand DNA; Vascular endothelial growth factor (VEGF)