title: Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers.
abstract: BACKGROUND
  There is insufficient knowledge about the chondrocyte membranome and its molecular composition.
  
  
  OBJECTIVE
  To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins.
  
  
  METHODS
  Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic fractions; trypsin-digested proteins were analysed by nanoLC-MS/MS.
  
  
  RESULTS
  A total of 315 proteins were identified. The phase extraction method yielded a high proportion of membrane proteins (56%) including CD276, S100-A6 and three VDAC isoforms.
  
  
  CONCLUSIONS
  Defining the chondrocyte membranome is likely to reveal new biomarker targets for conventional and biological drug discovery.
authors: a Department of Veterinary Preclinical Sciences , School of Veterinary Medicine, Faculty of Health and Medical Sciences, University of Surrey , Guildford , Surrey , UK .|b Department of Anatomy , Histology and Embryology, Faculty of Medicine, University of Debrecen , Debrecen , Hungary .
authors: Csaba
authors: C
authors: Matta
authors: c Proteomics Laboratory, School of Biosciences, University of Nottingham , Sutton Bonington , UK .
authors: Xiaofei
authors: X
authors: Zhang
authors: c Proteomics Laboratory, School of Biosciences, University of Nottingham , Sutton Bonington , UK .
authors: Susan
authors: S
authors: Liddell
authors: d Bruker UK Limited , Coventry , UK .
authors: Julia R
authors: JR
authors: Smith
authors: a Department of Veterinary Preclinical Sciences , School of Veterinary Medicine, Faculty of Health and Medical Sciences, University of Surrey , Guildford , Surrey , UK .|e Arthritis Research UK Centre for Sport, Exercise and Osteoarthritis, Arthritis Research UK Pain Centre, Medical Research Council and Arthritis Research UK Centre for Musculoskeletal Ageing Research, University of Nottingham, Queen's Medical Centre , Nottingham , UK , and.|f Center of Excellence in Genomic Medicine Research (CEGMR), King Fahd Medical Research Centre (KFMRC), Faculty of Applied Medical Sciences, King AbdulAziz University , Jeddah , Kingdom of Saudi Arabia.
authors: Ali
authors: A
authors: Mobasheri
mesh_terms: D000818:Animals; D060887:B7 Antigens / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D019295:Computational Biology; D003902:Detergents / Q000737:chemistry; D006736:Horses; D057927:Hydrophobic and Hydrophilic Interactions; D059625:Liquid-Liquid Extraction; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008662:Metacarpophalangeal Joint / Q000166:cytology / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D009418:S100 Proteins / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism
chemical_list: D060887:B7 Antigens; D015415:Biomarkers; D003902:Detergents; D008565:Membrane Proteins; D009418:S100 Proteins; D050994:Voltage-Dependent Anion Channels; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40
keywords: Articular cartilage; Triton X-114; chondrocyte; equine; mass spectrometry; membrane protein extraction; membranome; proteomics