title: Whole genome sequence analysis of BT-474 using complete Genomics' standard and long fragment read technologies.
abstract: BACKGROUND
  The cell line BT-474 is a popular cell line for studying the biology of cancer and developing novel drugs. However, there is no complete, published genome sequence for this highly utilized scientific resource. In this study we sought to provide a comprehensive and useful data set for the scientific community by generating a whole genome sequence for BT-474.
  
  
  RESULTS
  Five μg of genomic DNA, isolated from an early passage of the BT-474 cell line, was used to generate a whole genome sequence (114X coverage) using Complete Genomics' standard sequencing process. To provide additional variant phasing and structural variation data we also processed and analyzed two separate libraries of 5 and 6 individual cells to depths of 99X and 87X, respectively, using Complete Genomics' Long Fragment Read (LFR) technology.
  
  
  CONCLUSIONS
  BT-474 is a highly aneuploid cell line with an extremely complex genome sequence. This ~300X total coverage genome sequence provides a more complete understanding of this highly utilized cell line at the genomic level.
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA.
authors: Serban
authors: S
authors: Ciotlos
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA.
authors: Qing
authors: Q
authors: Mao
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA.
authors: Rebecca Yu
authors: RY
authors: Zhang
authors: BGI-Shenzhen, Shenzhen, 518083 China.
authors: Zhenyu
authors: Z
authors: Li
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA.
authors: Robert
authors: R
authors: Chin
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA.
authors: Natali
authors: N
authors: Gulbahce
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA.
authors: Sophie Jia
authors: SJ
authors: Liu
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA ; BGI-Shenzhen, Shenzhen, 518083 China.
authors: Radoje
authors: R
authors: Drmanac
authors: Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043 USA ; BGI-Shenzhen, Shenzhen, 518083 China.
authors: Brock A
authors: BA
authors: Peters
mesh_terms: D000782:Aneuploidy; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics*; D059472:Exome / Q000235:genetics; D005260:Female; D015894:Genome, Human / Q000235:genetics*; D015698:Genomic Library; D023281:Genomics / Q000379:methods*; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000379:methods*; D014178:Translocation, Genetic
chemical_list: D004273:DNA, Neoplasm
keywords: BT-474; BT474; Breast cancer; Complete Genomics; Long Fragment Read; Whole genome sequencing