title: Comparative study of muscle proteins in relation to the development of yake in three tropical tuna species yellowfin (Thunnus albacares), big eye (Thunnus obesus) and skipjack (Katsuwonus pelamis).
abstract: Burnt tuna (BT), or yake-niku, is a quality flaw of the muscle characterised by a pale colour and grainy and exudative texture. Cathepsin-L, water soluble and total protein components from normal and BT muscles, from three tropical tuna species - yellowfin (YFT, Thunnus albacares), bigeye (BET, Thunnus obesus) and skipjack (SKJ, Katsuwonus pelamis) - were compared by electrophoretic and western blot analyses to identify biomarkers for BT. As expected, SDS-PAGE patterns were species-specific but differences, due to BT, were observed only between some low ionic strength extracts of BET and YFT. Protein oxidation and cell proliferation analysed by immunoblotting did not show differences between BT and normal muscles. Gelatine zymography revealed different gelatinase activity patterns that, although not linked to BT, may affect the final texture of the muscle. A 43 kDa band, identified as creatine kinase by proteomic analysis, showed the potential to be a good indicator for BT in BET and YFT.
authors: Research Center for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (EHU-UPV), Areatza z/g, E-48620 Plenztia, Bizkaia, Spain.
authors: Oihane
authors: O
authors: Erdaide
authors: Research Center for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (EHU-UPV), Areatza z/g, E-48620 Plenztia, Bizkaia, Spain.
authors: Xabier
authors: X
authors: Lekube
authors: Norwegian College of Fishery Science, UiT - The Arctic University of Norway, N-9037 Tromsø, Norway.
authors: Ragnar L
authors: RL
authors: Olsen
authors: Department of Fisheries Management and Marine Research, Echebastar Fleet S.L.U., Muelle Erroxape s/n (Box 39), 48370 Bermeo, Spain.
authors: Unai
authors: U
authors: Ganzedo
authors: Research Center for Experimental Marine Biology and Biotechnology (PIE), University of the Basque Country (EHU-UPV), Areatza z/g, E-48620 Plenztia, Bizkaia, Spain; Norwegian College of Fishery Science, UiT - The Arctic University of Norway, N-9037 Tromsø, Norway; IKERBASQUE, Basque Foundation for Science, E-48011 Bilbao, Bizkaia, Spain. Electronic address: iciar.martinez@ehu.eus.
authors: Iciar
authors: I
authors: Martinez
mesh_terms: D000818:Animals; D005399:Fishes / Q000254:growth & development* / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D040901:Proteomics; D014413:Tuna / Q000254:growth & development* / Q000378:metabolism
chemical_list: D009124:Muscle Proteins
keywords: Biomarker; Burnt tuna; Creatine kinase; Muscle; Oxidation; Proteases; Tuna fish quality; Yake