title: Electrocatalytic Currents from Single Enzyme Molecules.
abstract: Single molecule enzymology provides an opportunity to examine details of enzyme mechanisms that are not distinguishable in biomolecule ensemble studies. Here we report, for the first time, detection of the current produced in an electrocatalytic reaction by a single redox enzyme molecule when it collides with an ultramicroelectrode. The catalytic process provides amplification of the current from electron-transfer events at the catalyst leading to a measurable current. This new methodology monitors turnover of a single enzyme molecule. The methodology might complement existing single molecule techniques, giving further insights into enzymatic mechanisms and filling the gap between fundamental understanding of biocatalytic processes and their potential for bioenergy production.
authors: Department of Physics, Chemistry and Biology, Linköping University , SE-581 83, Linköping, Sweden.
authors: Alina N
authors: AN
authors: Sekretaryova
authors: Department of Physics, Chemistry and Biology, Linköping University , SE-581 83, Linköping, Sweden.|Department of Science and Technology, Linköping University , SE-601 74, Norrköping, Sweden.
authors: Mikhail Yu
authors: MY
authors: Vagin
authors: Department of Physics, Chemistry and Biology, Linköping University , SE-581 83, Linköping, Sweden.
authors: Anthony P F
authors: AP
authors: Turner
authors: Department of Physics, Chemistry and Biology, Linköping University , SE-581 83, Linköping, Sweden.
authors: Mats
authors: M
authors: Eriksson
mesh_terms: D055162:Biocatalysis; D004563:Electrochemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism*; D008956:Models, Chemical; D008958:Models, Molecular; D010084:Oxidation-Reduction; D020072:Polyporales / Q000201:enzymology; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D005656:Fungal Proteins; D042845:Laccase