title: Gene Expression Profiling Supports the Neural Crest Origin of Adult Rodent Carotid Body Stem Cells and Identifies CD10 as a Marker for Mesectoderm-Committed Progenitors.
abstract: Neural stem cells (NSCs) are promising tools for understanding nervous system plasticity and repair, but their use is hampered by the lack of markers suitable for their prospective isolation and characterization. The carotid body (CB) contains a population of peripheral NSCs, which support organ growth during acclimatization to hypoxia. We have set up CB neurosphere (NS) cultures enriched in differentiated neuronal (glomus) cells versus undifferentiated progenitors to investigate molecular hallmarks of cell classes within the CB stem cell (CBSC) niche. Microarray gene expression analysis in NS is compatible with CBSCs being neural crest derived-multipotent progenitor cells able to sustain CB growth upon exposure to hypoxia. Moreover, we have identified CD10 as a marker suitable for isolation of a population of CB mesectoderm-committed progenitor cells. CD10 + cells are resting in normoxia, and during hypoxia they are activated to proliferate and to eventually complete maturation into mesectodermal cells, thus participating in the angiogenesis necessary for CB growth. Our results shed light into the molecular and cellular mechanisms involved in CBSC fate choice, favoring a potential use of these cells for cell therapy. Stem Cells 2016;34:1637-1650.
authors: Departamento de Fisiología Médica y Biofísica, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Spain.
authors: Elena
authors: E
authors: Navarro-Guerrero
authors: Departamento de Fisiología Médica y Biofísica, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Spain.
authors: Aida
authors: A
authors: Platero-Luengo
authors: Departamento de Fisiología Médica y Biofísica, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Spain.
authors: Pedro
authors: P
authors: Linares-Clemente
authors: Bioinformatics and Computational Biology Group. Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Spain.
authors: Ildefonso
authors: I
authors: Cases
authors: Departamento de Fisiología Médica y Biofísica, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Spain.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Spain.
authors: José
authors: J
authors: López-Barneo
authors: Departamento de Fisiología Médica y Biofísica, Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Spain.
authors: Ricardo
authors: R
authors: Pardal
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002344:Carotid Body / Q000166:cytology*; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics; D015687:Cell Hypoxia / Q000235:genetics; D019070:Cell Lineage; D004475:Ectoderm / Q000166:cytology*; D019332:Endothelin-1 / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008648:Mesoderm / Q000166:cytology*; D008822:Mice, Transgenic; D015260:Neprilysin / Q000378:metabolism*; D009432:Neural Crest / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017208:Rats, Wistar; D018874:Spheroids, Cellular / Q000166:cytology; D055153:Stem Cell Niche / Q000235:genetics
chemical_list: D015415:Biomarkers; D019332:Endothelin-1; D015260:Neprilysin
keywords: Adult neural stem cells; Angiogenesis; CD10; Carotid body; Hypoxia; Neural crest