title: THBS1 is induced by TGFB1 in the cancer stroma and promotes invasion of oral squamous cell carcinoma.
abstract: BACKGROUND
  THBS1 (thrombospondin-1) is the extracellular matrix (ECM) protein that affects diverse cellular activities. It constitutes the tumor stroma, but the role of THBS1 in oral squamous cell carcinoma (OSCC) development is unclear. The aim of this study was to clarify the relevance of THBS1 in the pathogenesis of OSCC.
  
  
  METHODS
  The expression of THBS1 was examined in 44 OSCC by immunohistochemical analysis and in 43 OSCC by cDNA microarray analysis. Cell culture experiments were conducted using human OSCC cell lines HSC3 and HO1N1 and mouse fibroblast ST2 cells to examine the effect of TGFB1 on THBS1 expression, and the effect of THBS1 on cellular behaviors.
  
  
  RESULTS
  THBS1 was specifically induced in the tumor microenvironment of OSCC. THBS1 appeared to be produced mainly by the stromal cells, but also by OSCC cells. TGFB1 stimulated THBS1 expression in ST2, primary fibroblasts, and the OSCC cells. THBS1 promoted migration and invasion of HSC3 and HO1N1 in transwell migration assays. THBS1 stimulated the expression of MMP3 (matrix metalloprotease 3), MMP9, MMP11, and MMP13 in ST2 cells and MMP3, MMP11, and MMP13 in HO1N1 cells. The RGD peptide suppressed the THBS1-stimulated migration and upregulation of MMP11 and MMP13.
  
  
  CONCLUSIONS
  THBS1 is a tumor-specific ECM protein that is induced by TGFB1 and promotes migration of cancer cells and stimulates the expression of MMPs partly through the integrin signaling, thereby favoring OSCC invasion.
authors: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.|Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Samir Kumar
authors: SK
authors: Pal
authors: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Chi Thi Kim
authors: CT
authors: Nguyen
authors: Department of Oral Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Kei-Ichi
authors: KI
authors: Morita
authors: Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Tokyo, Japan.|Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Yoshio
authors: Y
authors: Miki
authors: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Kou
authors: K
authors: Kayamori
authors: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.|Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Tokyo, Japan.|Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
authors: Akira
authors: A
authors: Yamaguchi
authors: Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. s-kei.mpa@tmd.ac.jp.
authors: Kei
authors: K
authors: Sakamoto
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018076:DNA, Complementary / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008666:Metalloendopeptidases / Q000096:biosynthesis; D051379:Mice; D009062:Mouth Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D000077195:Squamous Cell Carcinoma of Head and Neck; D017154:Stromal Cells / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D019700:Thrombospondin 1 / Q000096:biosynthesis*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D019700:Thrombospondin 1; D053773:Transforming Growth Factor beta1; C000611726:thrombospondin-1, human; C585483:Thbs1 protein, mouse; D008666:Metalloendopeptidases
keywords: TGFB1; THBS1; TSP-1; integrin; oral cancer; thrombospondin