title: Investigation of a suspected nosocomial transmission of blaKPC3-mediated carbapenem-resistant Klebsiella pneumoniae by whole genome sequencing.
abstract: Whole genome sequencing (WGS) was compared to pulse-field gel electrophoresis (PFGE) of XbaI-digested genomic DNA, as methods by which to evaluate a potential transmission of carbapenem-resistant Klebsiella pneumoniae between 2 hospital inpatients. PFGE result demonstrated only 1-band difference between the isolates, suggesting probable relatedness. In contrast, while WGS data demonstrated the same sequence type and very similar chromosomal sequences, over 20 single nucleotide variants were identified between the isolates, bringing into question whether there was a transmission event. WGS also identified an additional plasmid, with an XbaI restriction site in the isolates of the second patient that was not identified by PFGE. While WGS provided additional information that was not available by PFGE, in this study, neither method could definitively conclude the relatedness between the isolates.
authors: Pathology & Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors: Shangxin
authors: S
authors: Yang
authors: Pathology & Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors: Peera
authors: P
authors: Hemarajata
authors: Pathology & Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors: Janet
authors: J
authors: Hindler
authors: Pathology & Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors: Kevin
authors: K
authors: Ward
authors: Mi Next Generation Science (MiNGS) Core Laboratory, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors: Helty
authors: H
authors: Adisetiyo
authors: Mi Next Generation Science (MiNGS) Core Laboratory, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors: Fan
authors: F
authors: Li
authors: Mi Next Generation Science (MiNGS) Core Laboratory, Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors: Grace M
authors: GM
authors: Aldrovandi
authors: Los Angeles County Public Health Laboratory, Downey, CA, USA.
authors: Nicole M
authors: NM
authors: Green
authors: Division of Infectious Diseases, UCLA, Los Angeles, CA, USA.
authors: Dana
authors: D
authors: Russell
authors: Division of Infectious Diseases, UCLA, Los Angeles, CA, USA.
authors: Zachary
authors: Z
authors: Rubin
authors: Pathology & Laboratory Medicine, UCLA, Los Angeles, CA, USA.
authors: Romney M
authors: RM
authors: Humphries
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D003428:Cross Infection / Q000453:epidemiology / Q000635:transmission*; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000635:transmission*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D017720:Molecular Epidemiology; D058889:Molecular Typing / Q000379:methods; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C495220:beta-lactamase KPC-3, Klebsiella pneumoniae
keywords: CRE; CRKP; Carbapenem resistance; Klebsiella pneumoniae; Mutation; NGS; Nosocomial transmission; PFGE; SNV; Single nucleotide variant; Tn4401; WGS; Whole genome sequencing