title: Low-level laser irradiation effect on endothelial cells under conditions of hyperglycemia.
abstract: Diabetes mellitus is considered to be a very serious lifestyle disease leading to cardiovascular complications and impaired wound healing observed in the diabetic foot syndrome. Chronic hyperglycemia is the source of the endothelial activation. The inflammatory process in diabetes is associated with the secretion of inflammatory cytokines by endothelial cells, e.g., tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6). The method of phototherapy using laser beam of low power (LLLT-low-level laser therapy) effectively supports the conventional treatment of diabetic vascular complications such as diabetic foot syndrome. The aim of our study was to evaluate the effect of low-power laser irradiation at two wavelengths (635 and 830 nm) on the secretion of inflammatory factors (TNF-α and IL-6) by the endothelial cell culture-HUVEC line (human umbilical vein endothelial cell)-under conditions of hyperglycemia. It is considered that adverse effects of hyperglycemia on vascular endothelial cells may be corrected by the action of LLLT, especially with the wavelength of 830 nm. It leads to the reduction of TNF-α concentration in the supernatant and enhancement of cell proliferation. Endothelial cells play an important role in the pathogenesis of diabetes; however, a small number of studies evaluate an impact of LLLT on these cells under conditions of hyperglycemia. Further work on this subject is warranted.
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Skłodowskiej-Curie Street No 9, Bydgoszcz, Poland. krzyg@cm.umk.pl.
authors: Krzysztof
authors: K
authors: Góralczyk
authors: Department of Laserotherapy and Physiotherapy, Faculty of Health Sciences, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
authors: Justyna
authors: J
authors: Szymańska
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Skłodowskiej-Curie Street No 9, Bydgoszcz, Poland.
authors: Katarzyna
authors: K
authors: Szot
authors: Department of Laserotherapy and Physiotherapy, Faculty of Health Sciences, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
authors: Jacek
authors: J
authors: Fisz
authors: Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Skłodowskiej-Curie Street No 9, Bydgoszcz, Poland.
authors: Danuta
authors: D
authors: Rość
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D016207:Cytokines / Q000528:radiation effects; D042783:Endothelial Cells / Q000528:radiation effects*; D006801:Humans; D006943:Hyperglycemia / Q000532:radiotherapy*; D015850:Interleukin-6 / Q000528:radiation effects*; D028022:Low-Level Light Therapy / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000528:radiation effects*; D014471:Umbilical Veins
chemical_list: D016207:Cytokines; C508600:IL6 protein, human; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
keywords: Endothelial cells; IL-6; Low-level laser therapy; TNF-alpha