title: One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum.
abstract: B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using one-step chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A545/A280) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits α and β and a low intensity band of 27 kDa assigned to γ subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level.
authors: College of Life Science, Yantai University, 30 Qingquan Street, Yantai 264005, China. Electronic address: zhhtang6@163.com.
authors: Zhihong
authors: Z
authors: Tang
authors: College of Life Science, Yantai University, 30 Qingquan Street, Yantai 264005, China.
authors: Jilu Zhao
authors: College of Life Science, Yantai University, 30 Qingquan Street, Yantai 264005, China.
authors: Bao
authors: B
authors: Ju
authors: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Street, Yantai 264003, China.
authors: Wenjun
authors: W
authors: Li
authors: College of Life Science, Yantai University, 30 Qingquan Street, Yantai 264005, China.
authors: Shaohong
authors: S
authors: Wen
authors: College of Agriculture, Ludong University, 186 Hongqizhong Street, Yantai 264025, China.
authors: Yang
authors: Y
authors: Pu
authors: Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Street, Yantai 264003, China.
authors: Song
authors: S
authors: Qin
mesh_terms: D000837:Anion Exchange Resins / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000191:economics / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D010799:Phycoerythrin / Q000737:chemistry / Q000302:isolation & purification*; D044665:Porphyridium / Q000737:chemistry*; D014461:Ultracentrifugation
chemical_list: D000837:Anion Exchange Resins; D010799:Phycoerythrin
keywords: B-phycoerythrin; Chromatography; Porphyridium cruentum; Purification