title: Transcriptome-Wide Identification and Expression Profiling Analysis of Chrysanthemum Trihelix Transcription Factors.
abstract: Trihelix transcription factors are thought to feature a typical DNA-binding trihelix (helix-loop-helix-loop-helix) domain that binds specifically to the GT motif, a light-responsive DNA element. Members of the trihelix family are known to function in a number of processes in plants. Here, we characterize 20 trihelix family genes in the important ornamental plant chrysanthemum (Chrysanthemum morifolium). Based on transcriptomic data, 20 distinct sequences distributed across four of five groups revealed by a phylogenetic tree were isolated and amplified. The phylogenetic analysis also identified four pairs of orthologous proteins shared by Arabidopsis and chrysanthemum and five pairs of paralogous proteins in chrysanthemum. Conserved motifs in the trihelix proteins shared by Arabidopsis and chrysanthemum were analyzed using MEME, and further bioinformatic analysis revealed that 16 CmTHs can be targeted by 20 miRNA families and that miR414 can target 9 CmTHs. qPCR results displayed that most chrysanthemum trihelix genes were highly expressed in inflorescences, while 20 CmTH genes were in response to phytohormone treatments and abiotic stresses. This work improves our understanding of the various functions of trihelix gene family members in response to hormonal stimuli and stress.
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. aiping_song@aliyun.com.
authors: Aiping
authors: A
authors: Song
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2013104120@njau.edu.cn.
authors: Dan
authors: D
authors: Wu
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2013104100@njau.edu.cn.
authors: Qingqing
authors: Q
authors: Fan
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2015204031@njau.edu.cn.
authors: Chang
authors: C
authors: Tian
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chensm@njau.edu.cn.
authors: Sumei
authors: S
authors: Chen
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. guanzhy@njau.edu.cn.
authors: Zhiyong
authors: Z
authors: Guan
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2014104098@njau.edu.cn.
authors: Jingjing
authors: J
authors: Xin
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2014104099@njau.edu.cn.
authors: Kunkun
authors: K
authors: Zhao
authors: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chenfd@njau.edu.cn.
authors: Fadi
authors: F
authors: Chen
mesh_terms: D031202:Chrysanthemum / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Chrysanthemum morifolium; phylogenetic analysis; stress response; transcription pattern; trihelix