title: Overexpression of the NDR1/HIN1-Like Gene NHL6 Modifies Seed Germination in Response to Abscisic Acid and Abiotic Stresses in Arabidopsis.
abstract: NHL (NDR1/HIN1-like) genes play crucial roles in pathogen induced plant responses to biotic stress. Here, we report the possible function of NHL6 in plant response to abscisic acid (ABA) and abiotic stress. NHL6 was highly expressed in non-germinated seeds, and its expression was strongly induced by ABA and multiple abiotic stress signals. Loss-of-function of NHL6 decreased sensitivity to ABA in the early developmental stages including seed germination and post-germination seedling growth of the nhl6 mutants. However, overexpression of NHL6 increased sensitivity to ABA, salt and osmotic stress of the transgenic plants. Further studies indicated that the increased sensitivity in the 35S::NHL6 overexpressing plants could be a result of both ABA hypersensitivity and increased endogenous ABA accumulation under the stress conditions. It was also seen that the ABA-responsive element binding factors AREB1, AREB2 and ABF3 could regulate NHL6 expression at transcriptional level. Our results indicate that NHL6 plays an important role in the abiotic stresses-induced ABA signaling and biosynthesis, particularly during seed germination and early seedling development in Arabidopsis.
authors: College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China.|National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.|Plant Sciences Institute and the Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011, United States of America.
authors: Yan
authors: Y
authors: Bao
authors: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Wei-Meng
authors: WM
authors: Song
authors: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Jing
authors: J
authors: Pan
authors: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Chun-Mei
authors: CM
authors: Jiang
authors: Plant Sciences Institute and the Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011, United States of America.
authors: Renu
authors: R
authors: Srivastava
authors: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Bei
authors: B
authors: Li
authors: College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China.
authors: Lu-Ying
authors: LY
authors: Zhu
authors: College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China.
authors: Hong-Yan
authors: HY
authors: Su
authors: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Xiao-Shu
authors: XS
authors: Gao
authors: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Hua
authors: H
authors: Liu
authors: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Xiang
authors: X
authors: Yu
authors: College of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing, 210093, China.
authors: Lei
authors: L
authors: Yang
authors: College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China.
authors: Xian-Hao
authors: XH
authors: Cheng
authors: College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China.|National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China.
authors: Hong-Xia
authors: HX
authors: Zhang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009997:Osmotic Pressure; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D008565:Membrane Proteins; D012965:Sodium Chloride; D014157:Transcription Factors; C506773:ABF3 protein, Arabidopsis; C508877:At1g45249 protein, Arabidopsis; C576624:AREB2 protein, Arabidopsis; C109369:NDR1 protein, Arabidopsis; C000609142:NHL6 protein, Arabidopsis