title: Determination of ractopamine in pork using a magnetic molecularly imprinted polymer as adsorbent followed by HPLC.
abstract: A new magnetic molecularly imprinted polymers (MMIPs) for separation and concentration of ractopamine (RAC) were prepared using surface molecular imprinting technique with methacryloyl chloride as functional monomer and RAC as template. The MMIPs were characterized using transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometer. The results of re-binding experiments indicated that the MMIPs had fast adsorption kinetics and could reach binding equilibrium within 20 min, and the adsorption capacity of the MMIPs was 2.87-fold higher than that of the corresponding non-imprinted polymer. The selectivity of the MMIPs was evaluated according to its recognition to RAC and its analogues. The synthesized MMIPs were successfully applied to extraction, followed by high performance liquid chromatography to determine RAC in real food samples. Spiked recoveries ranged from 73.60% to 94.5%, with relative standard deviations of <11.17%.
authors: College of Food Science and Project Engineering, Bohai University, Jinzhou 121013, China. Electronic address: tangyiwei81@163.com.
authors: Yiwei
authors: Y
authors: Tang
authors: College of Food Science and Project Engineering, Bohai University, Jinzhou 121013, China.
authors: Jingwen
authors: J
authors: Gao
authors: College of Food Science and Project Engineering, Bohai University, Jinzhou 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai University, Jinzhou 121013, China.
authors: Xiuying
authors: X
authors: Liu
authors: College of Food Science and Project Engineering, Bohai University, Jinzhou 121013, China.
authors: Jianxing
authors: J
authors: Lan
authors: Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China. Electronic address: huaxue_gaoxue@126.com.
authors: Xue
authors: X
authors: Gao
authors: Food Safety Key Lab of Liaoning Province, Bohai University, Jinzhou 121013, China.
authors: Yong
authors: Y
authors: Ma
authors: College of Food Science and Project Engineering, Bohai University, Jinzhou 121013, China.
authors: Min
authors: M
authors: Li
authors: National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai University, Jinzhou 121013, China. Electronic address: lijr6491@163.com.
authors: Jianrong
authors: J
authors: Li
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054802:Molecular Imprinting / Q000379:methods*; D010627:Phenethylamines / Q000032:analysis*; D011108:Polymers / Q000737:chemistry*; D000069466:Red Meat / Q000032:analysis*; D013552:Swine
chemical_list: D010627:Phenethylamines; D011108:Polymers; C060897:ractopamine
keywords: Extraction; HPLC; Magnetic molecularly imprinted polymer; Pork; Ractopamine