title: Islet Culture/Preservation Before Islet Transplantation.
abstract: Although islet culture prior to transplantation provides flexibility for the evaluation of isolated islets and the pretreatment of patients, it is well known that isolated islets deteriorate rapidly in culture. Human serum albumin (HSA) is used for medium supplementation instead of fetal bovine serum (FBS), which is typically used for islet culture research, to avoid the introduction of xenogeneic materials. However, FBS contains several factors that are beneficial to islet viability and which also neutralize the endogenous pancreatic enzymes or exogenous enzymes left over from the isolation process. Several groups have reported the comparison of cultures at 22°C and 37°C. Recent studies have demonstrated the superiority of 4°C preservation to 22°C and 37°C cultures. We herein review the current research on islet culture/preservation for clinical islet transplantation.
authors: Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
authors: Hirofumi
authors: H
authors: Noguchi
authors: Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
authors: Chika
authors: C
authors: Miyagi-Shiohira
authors: Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
authors: Kiyoto
authors: K
authors: Kurima
authors: † Okayama Saidaiji Hospital , Okayama , Japan.
authors: Naoya
authors: N
authors: Kobayashi
authors: ‡ Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Niigata , Japan.
authors: Issei
authors: I
authors: Saitoh
authors: § Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Masami
authors: M
authors: Watanabe
authors: ¶ Department of Socio-environmental Design, Hiroshima International University , Hiroshima , Japan.
authors: Yasufumi
authors: Y
authors: Noguchi
authors: # Department of Molecular and Cellular Physiology, Graduate School of Medicine, University of the Ryukyus , Okinawa , Japan.
authors: Masayuki
authors: M
authors: Matsushita
keywords: Culture condition; Islet transplantation; Low temperature; Preservation solution; University of Wisconsin (UW) solution