title: Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus.
abstract: Microsatellite sequences were isolated from enriched genomic libraries of the spotted spiny lobster, Panulirus guttatus using 454 pyrosequencing. Twenty-nine previously developed polymerase chain reaction primer pairs of Panulirus argus microsatellite loci were also tested for cross-species amplification in Panulirus guttatus. In total, eight consistently amplifying, and polymorphic loci were characterized for 57 individuals collected in the Florida Keys and Bermuda. The number of alleles per locus ranged from 8 to 20 and observed heterozygosities ranged from 0.409 to 0.958. Significant deviations from Hardy-Weinberg equilibrium were found in one locus from Florida and three loci from Bermuda. Quality control testing indicated that all loci were easy to score, highly polymorphic and showed no evidence of linkage disequilibrium. Null alleles were detected in three loci with moderate frequencies ranging from (20% to 22%). These eight microsatellites provide novel molecular markers for future conservation genetics research of P. guttatus.
authors: Faculty of Life Sciences, University of Manchester, Manchester, England; Current affiliation: Smithsonian Museum of Natural History, Smithsonian Marine Station, Fort Pierce, FL, United States.
authors: Nathan
authors: N
authors: Truelove
authors: School of Forest Resources and Conservation & Emerging Pathogens Institute, University of Florida , Gainesville, FL , United States.
authors: Donald C
authors: DC
authors: Behringer
authors: Department of Biological Sciences, Old Dominion University , Norfolk, VA , United States.
authors: Mark J
authors: MJ
authors: Butler Iv
authors: Faculty of Life Sciences, University of Manchester , Manchester , England.
authors: Richard F
authors: RF
authors: Preziosi
keywords: Connectivity; Conservation; Neutral marker; Population genetics