title: Measurement of filter paper activities of cellulase with microplate-based assay.
abstract: It is always a challenge to determine the total cellulase activity efficiently without reducing accuracy. The most common total cellulase activity assay is the filter paper assay (FPA) established by the International Union of Pure and Applied Chemistry (IUPAC). A new procedure to measure the FPA with microplate-based assay was studied in this work, which followed the main idea of IUPAC to dilute cellulase preparation to get fixed glucose release. FPAs of six cellulase preparations were determined with the microplate-based assay. It is shown that FPAs of cellulase Youtell, RCconc, R-10, Lerkam, Yishui and Sinopharm were 67.9, 46.0, 46.1, 27.4, 7.6 and 8.0 IU/ml respectively. There was no significant difference at the 95% confidence level between the FPA determined with IUPAC and the microplate-based assay. It could be concluded that the FPA could be determined by the microplate-based assay with the same accuracy and much more efficiency compared with that by IUPAC.
authors: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, China.
authors: Xiaoxiao
authors: X
authors: Yu
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Yan
authors: Y
authors: Liu
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Yuxiao
authors: Y
authors: Cui
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Qiyue
authors: Q
authors: Cheng
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Zaixiao
authors: Z
authors: Zhang
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Jia Hui
authors: JH
authors: Lu
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Qingfan
authors: Q
authors: Meng
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Lirong
authors: L
authors: Teng
authors: School of Life Sciences, Jilin University, Changchun, Jilin 130012, China.
authors: Xiaodong
authors: X
authors: Ren
keywords: Cellulase; Filter paper assay; IUPAC; Microplate-based assay