title: Ruminococcus gnavus E1 modulates mucin expression and intestinal glycosylation.
abstract: OBJECTIVE
  The molecular cross-talk between commensal bacteria and the gut play an important role in the maintenance of the intestinal homeostasis and general health. Here, we studied the impact of a major Gram-positive anaerobic bacterium of the human gut microbiota, that is, Ruminococcus gnavus on the glycosylation pattern and the production of intestinal mucus by the goblet cells.
  
  
  RESULTS
  Our results showed that R. gnavus E1 specifically increases the expression and the glycosylation level of the intestinal glyco-conjugates by goblet cells in the colonic mucosa of mono-associated mice with R. gnavus E1 as well as in human HT29-MTX cells. Such an effect was mediated through induction of the level of mRNA encoding for the major intestinal gel-forming mucin such as MUC2 and various glycosyltransferase enzymes.
  
  
  CONCLUSIONS
  This study demonstrates for the first time that R. gnavus E1 possess the ability to modulate the glycosylation profile of the glyco-conjugate molecules and mucus in goblet cells.
  
  
  CONCLUSIONS
  Furthermore, we demonstrated that R. gnavus E1 modified specifically the glycosylation pattern and MUC2 expression by means of a small soluble factor of peptidic nature (<3 kDa) and heat stable in the HT29-MTX cell.
authors: iSm2 UMR 7313, CNRS, Centrale Marseille, Aix Marseille Université, Marseille, France.
authors: F
authors: F
authors: Graziani
authors: iSm2 UMR 7313, CNRS, Centrale Marseille, Aix Marseille Université, Marseille, France.
authors: A
authors: A
authors: Pujol
authors: iSm2 UMR 7313, CNRS, Centrale Marseille, Aix Marseille Université, Marseille, France.
authors: C
authors: C
authors: Nicoletti
authors: UP 2012.10.120.EGEAL, Institut Polytechnique, La Salle Beauvais, France.
authors: S
authors: S
authors: Dou
authors: iSm2 UMR 7313, CNRS, Centrale Marseille, Aix Marseille Université, Marseille, France.
authors: M
authors: M
authors: Maresca
authors: iSm2 UMR 7313, CNRS, Centrale Marseille, Aix Marseille Université, Marseille, France.
authors: T
authors: T
authors: Giardina
authors: IMM UMR 7283, CNRS, Aix Marseille Université, Marseille, France.
authors: M
authors: M
authors: Fons
authors: iSm2 UMR 7313, CNRS, Centrale Marseille, Aix Marseille Université, Marseille, France.
authors: J
authors: J
authors: Perrier
mesh_terms: D000818:Animals; D003106:Colon / Q000378:metabolism / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D006031:Glycosylation; D020397:Goblet Cells / Q000378:metabolism / Q000382:microbiology; D019073:HT29 Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology; D007422:Intestines / Q000382:microbiology; D051379:Mice; D009077:Mucins / Q000378:metabolism*; D045857:Ruminococcus / Q000502:physiology*
chemical_list: D009077:Mucins
keywords: Ruminococcus gnavus; glycosylation; goblet cells; gut microbiota; host-microbiota interactions; intestinal epithelial cells IEC; mucin