title: Comparative Experimental Infection Study in Dogs with Ehrlichia canis, E. chaffeensis, Anaplasma platys and A. phagocytophilum.
abstract: Dogs acquire infections with the Anaplasmataceae family pathogens, E. canis, E. chaffeensis, E. ewingii, A. platys and A. phagocytophilum mostly during summer months when ticks are actively feeding on animals. These pathogens are also identified as causing diseases in people. Despite the long history of tick-borne diseases in dogs, much remains to be defined pertaining to the clinical and pathological outcomes of infections with these pathogens. In the current study, we performed experimental infections in dogs with E. canis, E. chaffeensis, A. platys and A. phagocytophilum. Animals were monitored for 42 days to evaluate infection-specific clinical, hematological and pathological differences. All four pathogens caused systemic persistent infections detectible throughout the 6 weeks of infection assessment. Fever was frequently detected in animals infected with E. canis, E. chaffeensis, and A. platys, but not in dogs infected with A. phagocytophilum. Hematological differences were evident in all four infected groups, although significant overlap existed between the groups. A marked reduction in packed cell volume that correlated with reduced erythrocytes and hemoglobin was observed only in E. canis infected animals. A decline in platelet numbers was common with E. canis, A. platys and A. phagocytophilum infections. Histopathological lesions in lung, liver and spleen were observed in all four groups of infected dogs; infection with E. canis had the highest pathological scores, followed by E. chaffeensis, then A. platys and A. phagocytophilum. All four pathogens induced IgG responses starting on day 7 post infection, which was predominantly comprised of IgG2 subclass antibodies. This is the first detailed investigation comparing the infection progression and host responses in dogs after inoculation with four pathogens belonging to the Anaplasmataceae family. The study revealed a significant overlap in clinical, hematological and pathological changes resulting from the infections.
authors: Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America.
authors: Arathy D S
authors: AD
authors: Nair
authors: Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America.
authors: Chuanmin
authors: C
authors: Cheng
authors: Kansas State Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America.
authors: Chanran K
authors: CK
authors: Ganta
authors: Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America.
authors: Michael W
authors: MW
authors: Sanderson
authors: Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, United States of America.
authors: Arthur R
authors: AR
authors: Alleman
authors: Department of Entomology, University of Minnesota, St. Paul, Minnesota, United States of America.
authors: Ulrike G
authors: UG
authors: Munderloh
authors: Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America.
authors: Roman R
authors: RR
authors: Ganta
mesh_terms: D000709:Anaplasma / Q000276:immunology* / Q000472:pathogenicity; D000712:Anaplasmosis / Q000382:microbiology*; D000818:Animals; D001792:Blood Platelets / Q000276:immunology; D004283:Dog Diseases / Q000276:immunology* / Q000382:microbiology; D004285:Dogs; D004536:Ehrlichia / Q000276:immunology* / Q000472:pathogenicity; D016873:Ehrlichiosis / Q000382:microbiology* / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D010976:Platelet Count; D013154:Spleen / Q000382:microbiology; D017282:Tick-Borne Diseases / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013987:Ticks / Q000382:microbiology
chemical_list: D007074:Immunoglobulin G