title: Elevated Toll-Like Receptor-Induced CXCL8 Secretion in Human Blood Basophils from Allergic Donors Is Independent of Toll-Like Receptor Expression Levels.
abstract: Human blood basophils have recently gained interest in addition to their function as allergic effector cells. Previous work suggests the involvement of innate immune mechanisms in the development and exacerbation of allergic responses, which might be mediated by basophils. We assayed the expression levels of Toll-like receptor (TLR) 1, 2, 4 and 6 on purified basophils from birch pollen-, house dust mite-, and non-allergic individuals. Additionally, we compared cytokine and chemokine secretion upon TLR stimulation in these basophil donor groups. Expression of TLR4 on the basophils of the allergic donor groups was decreased and CXCL8 secretion was elevated upon stimulation of TLR1/2 and TLR2/6 compared to the non-allergic donors. Decreased TLR expression and elevated CXCL8 secretion may represent possible mechanisms for aggravation of allergic symptoms in case of parasitic infection.
authors: Department of Molecular Biology, University of Salzburg, Salzburg, Austria.|Laboratory for Immunological and Molecular Cancer Research, Paracelsus Medical University, Salzburg, Austria.
authors: Markus
authors: M
authors: Steiner
authors: Department of Dermatology, Paracelsus Medical University, Salzburg, Austria.
authors: Thomas
authors: T
authors: Hawranek
authors: Bühlmann Laboratories AG, Schönenbuch, Switzerland.
authors: Michael
authors: M
authors: Schneider
authors: Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
authors: Fatima
authors: F
authors: Ferreira
authors: Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
authors: Jutta
authors: J
authors: Horejs-Hoeck
authors: Department of Molecular Biology, University of Salzburg, Salzburg, Austria.|Department of Neurology, Paracelsus Medical University, Salzburg, Austria.
authors: Andrea
authors: A
authors: Harrer
authors: Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
authors: Martin
authors: M
authors: Himly
mesh_terms: D000293:Adolescent; D000328:Adult; D001491:Basophils / Q000276:immunology* / Q000473:pathology; D002648:Child; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000473:pathology; D016209:Interleukin-8 / Q000032:analysis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D051194:Toll-Like Receptor 1 / Q000032:analysis / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000032:analysis / Q000276:immunology; D051197:Toll-Like Receptor 4 / Q000032:analysis / Q000276:immunology; D051216:Toll-Like Receptor 6 / Q000032:analysis / Q000276:immunology; D051193:Toll-Like Receptors / Q000032:analysis / Q000276:immunology*; D055815:Young Adult
chemical_list: D016209:Interleukin-8; D051194:Toll-Like Receptor 1; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051216:Toll-Like Receptor 6; D051193:Toll-Like Receptors