title: Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis.
abstract: OBJECTIVE
  Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium.
  
  
  METHODS
  Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium.
  
  
  RESULTS
  TNFα significantly increased C1M up to seven-fold (p = 0.0002), C3M up to 24-fold (p = 0.0011), and acMMP3 up to 14-fold (p < 0.0001) in SMEs. IL-1β also significantly increased C1M up to five-fold (p = 0.00094), C3M four-fold (p = 0.007), and acMMP3 18-fold (p < 0.0001) in SMEs.
  
  
  CONCLUSIONS
  The biomarkers C1M, C3M, and acMMP-3 were synovitis biomarkers ex vivo and provide a translational tool together with the SME model.
authors: a Department of Rheumatology , Biomarkers and Research , Nordic Bioscience , Herlev , Denmark and.
authors: Cecilie
authors: C
authors: Kjelgaard-Petersen
authors: a Department of Rheumatology , Biomarkers and Research , Nordic Bioscience , Herlev , Denmark and.
authors: Anne Sofie
authors: AS
authors: Siebuhr
authors: b Orthopaedic Surgery Unit, Gentofte University Hospital , Gentofte , Denmark.
authors: Thorbjørn
authors: T
authors: Christiansen
authors: a Department of Rheumatology , Biomarkers and Research , Nordic Bioscience , Herlev , Denmark and.
authors: Christoph
authors: C
authors: Ladel
authors: a Department of Rheumatology , Biomarkers and Research , Nordic Bioscience , Herlev , Denmark and.
authors: Morten
authors: M
authors: Karsdal
authors: a Department of Rheumatology , Biomarkers and Research , Nordic Bioscience , Herlev , Denmark and.
authors: Anne-Christine
authors: AC
authors: Bay-Jensen
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology; D004789:Enzyme Activation; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D006801:Humans; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism*; D020370:Osteoarthritis, Knee / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D010446:Peptide Fragments / Q000378:metabolism*; D013583:Synovial Membrane / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D013585:Synovitis / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D013997:Time Factors; D046509:Tissue Culture Techniques; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D024061:Collagen Type III; D016207:Cytokines; D010446:Peptide Fragments; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3
keywords: Neo-epitope biomarkers; personalized medicine; synovial membrane