title: Isolated neonatal rat papillary muscles: a new model to translate neonatal rat myocyte signaling into contractile mechanics.
abstract: Isolated cardiac tissue allows investigators to study mechanisms underlying normal and pathological conditions, which would otherwise be difficult or impossible to perform in vivo. Cultured neonatal rat ventricular cardiac myocytes (NRVM) are widely used to study signaling and growth mechanisms in the heart, primarily due to the versatility, economy, and convenience of this in vitro model. However, the lack of a well-defined longitudinal cellular axis greatly hampers the ability to measure contractile function in these cells, and therefore to associate signaling with mechanical function. In these methods, we demonstrate that this limitation can be overcome by using papillary muscles isolated from neonatal rat hearts. In the methods we describe procedures for isolation of right ventricular papillary muscles from 3-day-old neonatal rats and effects of mechanical and humoral stimuli on contraction and relaxation properties of these tissues.
authors: Department of Medical Physiology, College of Medicine Texas A&M University System Health Science Center, Temple, Texas.
authors: Damir
authors: D
authors: Nizamutdinov
authors: Department of Medical Physiology, College of Medicine Texas A&M University System Health Science Center, Temple, Texas.
authors: Hao
authors: H
authors: Feng
authors: Department of Ophthalmology, UT Health Science Center San Antonio, San Antonio, Texas.
authors: Fnu
authors: F
authors: Gerilechaogetu
authors: Department of Medical Physiology, College of Medicine Texas A&M University System Health Science Center, Temple, Texas.
authors: Joseph A
authors: JA
authors: Dostal
authors: Central Texas Veterans Health Care System, Temple, Texas.
authors: Donald M
authors: DM
authors: Foster
authors: Central Texas Veterans Health Care System, Temple, Texas Division of Gastroenterology, Department of Internal Medicine, Baylor Scott & White Health Care System, Temple, Texas.
authors: Shannon S
authors: SS
authors: Glaser
authors: Department of Medical Physiology, College of Medicine Texas A&M University System Health Science Center, Temple, Texas Central Texas Veterans Health Care System, Temple, Texas ddostal@medicine.tamhsc.edu.
authors: David E
authors: DE
authors: Dostal
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004594:Electrophysiology / Q000379:methods; D009200:Myocardial Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods*; D010210:Papillary Muscles / Q000502:physiology*; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*
keywords: Cardiac contractility; isolated papillary muscle; neonatal cardiac tissue