title: Exploring chromatin organization mechanisms through its dynamic properties.
abstract: The organization of the genome in the nucleus is believed to be crucial for different cellular functions. It is known that chromosomes fold into distinct territories, but little is known about the mechanisms that maintain these territories. To explore these mechanisms, we used various live-cell imaging methods, including single particle tracking to characterize the diffusion properties of different genomic regions in live cells. Chromatin diffusion is found to be slow and anomalous; in vast contrast, depletion of lamin A protein significantly increases chromatin motion, and the diffusion pattern of chromatin transforms from slow anomalous to fast normal. More than this, depletion of lamin A protein also affects the dynamics of nuclear bodies. Our findings indicate that chromatin motion is mediated by lamin A and we suggest that constrained chromatin mobility allows to maintain chromosome territories. Thus, the discovery of this function of nucleoplasmic lamin A proteins sheds light on the maintenance mechanism of chromosome territories in the interphase nucleus, which ensures the proper function of the genome.
authors: a Physics Department and Nanotechnology Institute , Bar Ilan University , Ramat Gan , Israel.|b The Mina & Everard Goodman Faculty of Life Sciences and Nanotechnology Institute, Bar Ilan University , Ramat Gan , Israel.
authors: Irena
authors: I
authors: Bronshtein
authors: c Faculty of Engineering and Nanotechnology Institute, Bar Ilan University , Ramat Gan , Israel.
authors: Itamar
authors: I
authors: Kanter
authors: d Institut Curie, PSL Research University , CNRS, UMR 3125, INSERM, U 934, Paris , France.
authors: Eldad
authors: E
authors: Kepten
authors: a Physics Department and Nanotechnology Institute , Bar Ilan University , Ramat Gan , Israel.
authors: Moshe
authors: M
authors: Lindner
authors: a Physics Department and Nanotechnology Institute , Bar Ilan University , Ramat Gan , Israel.
authors: Shirly
authors: S
authors: Berezin
authors: b The Mina & Everard Goodman Faculty of Life Sciences and Nanotechnology Institute, Bar Ilan University , Ramat Gan , Israel.
authors: Yaron
authors: Y
authors: Shav-Tal
authors: a Physics Department and Nanotechnology Institute , Bar Ilan University , Ramat Gan , Israel.
authors: Yuval
authors: Y
authors: Garini
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D006801:Humans; D034904:Lamin Type A / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D034904:Lamin Type A
keywords: chromatin organization; diffusion; lamin A; nuclear structure; single particle tracking