title: [Influence of Microbial Metabolites of Phenolic Nature on the Activity of Mitochondrial Enzymes].
abstract: The aim of this work was to study the effect of microbial metabolites of phenolic nature on the activity of enzymes of the tricarboxylic acid cycle in isolated mitochondria, and determine metabolites of the tricarboxylic acid cycle as potential biomarkers of mitochondrial dysfunction in the blood of patients with sepsis. It is shown that microbial metabolites of phenolic nature have an inhibitory effect on the activity of dehydrogenases, determined by the reduction of dichlorophenolindophenol and nitroblue tetrazolium in liver mitochondria and liver homogenates. This effect is more pronounced in oxidation of the NAD-dependent substrates than succinate oxidation, and at lower concentrations of microbial metabolites than inhibition of respiration. By gas chromatography-mass spectrometry it was found that the content of the tricarboxylic acid cycle metabolites in the blood of patients with sepsis decreased compared to healthy donors. The data obtained show that the microbial phenolic acids can contribute significantly to the dysfunction of mitochondria and suppression of general metabolism, characteristic of these pathologies.
authors: N I
authors: NI
authors: Fedotcheva
authors: E G
authors: EG
authors: Litvinova
authors: A Aa
authors: AA
authors: Osipov
authors: A Yu
authors: AY
authors: Olenin
authors: V V
authors: VV
authors: Moroz
authors: N V
authors: NV
authors: Beloborodova
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002952:Citric Acid Cycle / Q000187:drug effects; D004579:Electron Transport; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010101:Oxygen Consumption; D010636:Phenols / Q000378:metabolism*; D051381:Rats; D012119:Respiration / Q000187:drug effects; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D010636:Phenols; D019802:Succinic Acid; D013385:Succinate Dehydrogenase