title: Click synthesis of quaternized poly(dimethylaminoethyl methacrylate) functionalized graphene oxide with improved antibacterial and antifouling ability.
abstract: A quaternized poly(dimethylaminoethyl methacrylate) functionalized graphene oxide (GO-QPDMAEMA) was successfully prepared in this study via click chemistry. Alkyne-functionalized graphene oxide (GO-alkyne) was first synthesized through a two-step amidation reaction of GO-COOH. Meanwhile, azide-terminated poly(dimethylaminoethyl methacrylate) (PDMAEMA-N3) was prepared via the atom-transfer radical-polymerization of dimethylaminoethyl methacrylate (DMAEMA). Subsequently, PDMAEMA-N3 was grafted onto the GO-alkyne through click chemistry to obtain PDMAEMA modified graphene oxide (GO-PDMAEMA). Finally, the tertiary amino groups of GO-PDMAEMA were quaternized by ethyl bromide to provide a quaternized poly(dimethylaminoethyl methacrylate) functionalized graphene oxide (GO-QPDMAEMA). Various characterization techniques, including Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, UV-vis spectrometry, ζ potential, Raman, contact angle analyses and field emission scanning electron microscope were used to ascertain the successful preparation of the quaternized GO-QPDMAEMA. Furthermore, antibacterial and antifouling activities of GO-QPDMAEMA were investigated via protein adsorption, as well as bacterial and cell adhesion studies. The results suggest that the GO-QPDMAEMA surface exhibited significant antibacterial and antifouling properties, compared with the GO-COOH and GO-PDMAEMA surfaces.
authors: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Qin
authors: Q
authors: Tu
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Chang
authors: C
authors: Tian
authors: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Tongtong
authors: T
authors: Ma
authors: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Long
authors: L
authors: Pang
authors: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: jywang@nwsuaf.edu.cn.
authors: Jinyi
authors: J
authors: Wang
mesh_terms: D000072283:A549 Cells; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D057219:Biofouling / Q000517:prevention & control; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D057930:Click Chemistry; D004337:Drug Carriers / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D006108:Graphite / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009757:Nylons / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry* / Q000494:pharmacology; D056951:Photoelectron Spectroscopy; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008689:Methacrylates; D009757:Nylons; D010087:Oxides; C407037:poly(2-(dimethylamino)ethyl methacrylate); D006108:Graphite
keywords: Antibacterial; Antifouling; Click chemistry; Graphene oxide; Quaternary ammonium