title: Dual effects of slightly acidic electrolyzed water (SAEW) treatment on the accumulation of γ-aminobutyric acid (GABA) and rutin in germinated buckwheat.
abstract: In the present study, the dual effects of slightly acidic electrolyzed water (SAEW) treatment on γ-aminobutyric acid (GABA) and rutin accumulation of germinated buckwheat were evaluated during germination. The results showed that SAEW treatment (pH 5.83, ACC of 20.3 mg/L) could promote the accumulation of GABA and rutin in germinated buckwheat. The GABA and rutin contents of SAEW-germinated buckwheat reached 143.20 and 739.9 mg/100 g respectively, which is significantly higher than those of control (P<0.05). Moreover, SAEW treatment could increase the activity of glutamic acid decarboxylase (GAD) and phenylalanine ammonialyase (PAL) and thus result in the GABA and rutin accumulation of germinated buckwheat. The results suggested that SAEW treatment could promote the rutin accumulation of germinated buckwheat by influencing phenylpropanoid secondary metabolic pathway instead of the inhibition of rutin degrading enzyme (RDE) activity. In addition, SAEW treatment had no adverse impact on the sprouts growth and could reduce the microbial populations of germinated buckwheat during germination.
authors: College of Bio Science and Engineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
authors: Jianxiong
authors: J
authors: Hao
authors: College of Bio Science and Engineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
authors: Tongjiao
authors: T
authors: Wu
authors: College of Bio Science and Engineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
authors: Huiying
authors: H
authors: Li
authors: Shijiazhuang Academy of Agricultural and Forestry Science, No. 70 Shenglibeilu, Shijiazhuang, Hebei 050000, PR China.
authors: Wei
authors: W
authors: Wang
authors: College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 40, No. 17 Qinghuadonglu, Haidian, Beijing 100083, PR China. Electronic address: liuhaijie@cau.edu.cn.
authors: Haijie
authors: H
authors: Liu
mesh_terms: D004572:Electrolysis / Q000379:methods*; D019612:Fagopyrum / Q000737:chemistry*; D018525:Germination / Q000187:drug effects*; D005968:Glutamate Decarboxylase / Q000737:chemistry*; D012431:Rutin / Q000737:chemistry* / Q000494:pharmacology; D014867:Water / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid
chemical_list: D014867:Water; D005680:gamma-Aminobutyric Acid; D012431:Rutin; D005968:Glutamate Decarboxylase
keywords: Buckwheat; Electrolyzed water; GABA; Germination; Rutin