title: On the Interaction Between Human IQGAP1 and Actin.
abstract: IQGAPs are eukaryotic proteins which integrate signals from various sources and pass these on the cytoskeleton. Understanding how they do this requires information on the interfaces between the proteins. Here, it is shown that the calponin homology domain of human IQGAP1 (CHD1) can be crosslinked with α-actin. The stoichiometry of the interaction was 1:1. A molecular model was built of the complex and associated bioinformatics analyses predicted that the interaction is likely to involve an electrostatic interaction between Lys-240 of α-actin and Glu-30 of CHD1. These residues are predicted to be accessible and are not involved in many intra-protein interactions; they are thus available for interaction with binding partners. They are both located in regions of the proteins which are predicted to be flexible and disordered; interactions between signalling molecules often involve flexible, disordered regions. The predicted binding region in CHD1 is well conserved in many eukaryotic IQGAP-like proteins. In some cases (e.g Dictyostelium discoideum and Saccharomyces cerevisiae) protein sequence conservation is weak, but molecular modelling reveals that a region of charged, polar residues in a flexible N-terminus is structurally well conserved. Therefore we conclude that the calponin homology domains of IQGAP1-like proteins interact initially through the electrostatic interaction identified here and that there may be subsequent conformational changes to form the final complex.
authors: Damian J
authors: DJ
authors: Magill
authors: Elaine
authors: E
authors: Hamilton
authors: Sally L
authors: SL
authors: Shirran
authors: Catherine H
authors: CH
authors: Botting
authors: School of Pharmacy and Biomolecular Sciences, University of Brighton, Huxley Building, Lewes Road, Brighton BN2 4GJ. UK. d.timson@brighton.ac.uk.
authors: David J
authors: DJ
authors: Timson
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D003432:Cross-Linking Reagents; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D000072417:Protein Domains; D020703:ras GTPase-Activating Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000199:Actins; D003432:Cross-Linking Reagents; C101203:IQ motif containing GTPase activating protein 1; D020703:ras GTPase-Activating Proteins; D005973:Glutamine; D008239:Lysine