title: In situ monitoring of Shewanella oneidensis MR-1 biofilm growth on gold electrodes by using a Pt microelectrode.
abstract: Much attention has been focused on electrochemically active bacteria (EAB) in the application of bioelectrochemical systems (BESs). Studying the EAB biofilm growth mechanism as well as electron transfer mechanism provides a route to upgrade BES performance. But an effective bacterial growth monitoring method on the biofilm scale is still absent in this field. In this work, electrode-attached bacterial biofilms formed by Shewanella oneidensis MR-1 were dynamically monitored through a microelectrode method. For S. oneidensis MR-1, a respiratory electron transport chain is associated with the secretion of riboflavin, severing as the cofactor to the outer membrane c-type cytochromes. The biofilm growth was monitored through adopting riboflavin as an electrochemical probe during the approach of the microelectrode to the biofilm external surface. This method allows in vivo and in situ biofilm monitoring at different growth stages without destructive manipulation. Furthermore, the biofilm growth monitoring results have been proved to be relatively accurate through observation under confocal laser scanning microscopy. We further applied this method to investigate the effects of four environmental factors (the concentrations of dissolved oxygen, sodium lactate, riboflavin as well as the electrode potential) on S. oneidensis MR-1 biofilm development.
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
authors: Han
authors: H
authors: Bao
authors: School of Environmental and Resource Science, Zhejiang A & F University, Hangzhou, China.
authors: Zhanwang
authors: Z
authors: Zheng
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
authors: Bin
authors: B
authors: Yang
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
authors: Ding
authors: D
authors: Liu
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
authors: Feifang
authors: F
authors: Li
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
authors: Xingwang
authors: X
authors: Zhang
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China. Electronic address: zdlizj@zju.edu.cn.
authors: Zhongjian
authors: Z
authors: Li
authors: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
authors: Lecheng
authors: L
authors: Lei
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms / Q000254:growth & development*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D008839:Microelectrodes; D010984:Platinum / Q000737:chemistry; D020592:Shewanella / Q000502:physiology*
chemical_list: D010984:Platinum; D006046:Gold
keywords: Bioelectrochemical system; Biofilm growth monitoring; Electrochemically active bacteria; Microelectrode