title: Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes.
abstract: A mild, fast, and sequence-independent method for controlled enzyme immobilization is presented. This novel approach involves the encapsulation of single-enzyme molecules and the covalent attachment of these nanobiocatalysts onto surfaces. Fast and mild immobilization conditions, combined with low nonspecific adsorption on hydrophobic substrates, enables well-defined surface patterns via microcontact printing. The biohybrid materials show enhanced activity in organic solvents.
authors: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.|Preparative Macromolecular Chemistry, Institute for Technical Chemistry and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 15, 76131, Karlsruhe, Germany.
authors: Ana
authors: A
authors: Beloqui
authors: Zoological Institute, Department of Cell and Neurobiology, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany.
authors: Sarah
authors: S
authors: Baur
authors: Institute for Applied Materials (IAM), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.|Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors: Vanessa
authors: V
authors: Trouillet
authors: Preparative Macromolecular Chemistry, Institute for Technical Chemistry and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 15, 76131, Karlsruhe, Germany.|Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.|Institute for Biological Interfaces (IBG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors: Alexander
authors: A
authors: Welle
authors: Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK.
authors: Jeppe
authors: J
authors: Madsen
authors: Zoological Institute, Department of Cell and Neurobiology, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany.|Institute for Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors: Martin
authors: M
authors: Bastmeyer
authors: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.|Preparative Macromolecular Chemistry, Institute for Technical Chemistry and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 15, 76131, Karlsruhe, Germany.
authors: Guillaume
authors: G
authors: Delaittre
mesh_terms: D000327:Adsorption; D055162:Biocatalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011327:Printing / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D006735:Horseradish Peroxidase
keywords: biocatalysis; encapsulation; immobilization; printable enzymes; thiol-ene