title: Yeast-generated virus-like particles as antigens for detection of human bocavirus 1-4 specific antibodies in human serum.
abstract: Human bocaviruses (HBoV) are non-enveloped, single-stranded DNA viruses, classified into the genus Bocavirus in the family Parvoviridae. Self-assembled virus-like particles (VLPs) composed of the major capsid protein VP2 of HBoV1-4 and mosaic VLPs composed of both VP2 and VP1 capsid proteins of HBoV1 were generated in yeast Saccharomyces cerevisiae and used to detect HBoV-specific IgG in human serum. Recombinant HBoV VLPs were similar to native HBoV particles in size and morphology. The prevalence of HBoV infection in a group of Lithuanian patients with clinical symptoms of respiratory tract infection was studied using purified yeast-generated VLPs as antigens in a competitive enzyme immunoassay (EIA). After depletion of cross-reactive antibodies, the seroprevalence of HBoV1 was 44.2 % and the seroprevalence of HBoV2-4 was 35.7 %. Mosaic VLPs consisting of HBoV1 VP1 and VP2 proteins showed a stronger reactivity with HBoV1 IgG-positive human serum specimens, and two equivocal serum specimens were reinterpreted as positive. Thus, mosaic VLPs offer a more sensitive tool for HBoV1 serology than currently available serodiagnostics tests based on VP2 VLPs. In conclusion, yeast S. cerevisiae represents an efficient expression system for generating recombinant HBoV1-4 VLPs of diagnostic relevance.
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania. paulius.tamosiunas@gmail.com.
authors: Paulius Lukas
authors: PL
authors: Tamošiūnas
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania.
authors: Rasa
authors: R
authors: Petraitytė-Burneikienė
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania.
authors: Aistė
authors: A
authors: Bulavaitė
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania.
authors: Kornelija
authors: K
authors: Marcinkevičiūtė
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania.
authors: Karolis
authors: K
authors: Simutis
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania.
authors: Rita
authors: R
authors: Lasickienė
authors: Clinic of Children's Diseases, Faculty of Medicine, Vilnius University, Vilnius, Lithuania.
authors: Regina
authors: R
authors: Firantienė
authors: Clinic of Children's Diseases, Faculty of Medicine, Vilnius University, Vilnius, Lithuania.
authors: Regina
authors: R
authors: Ėmužytė
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania.
authors: Aurelija
authors: A
authors: Žvirblienė
authors: Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241, Vilnius, Lithuania.
authors: Kęstutis
authors: K
authors: Sasnauskas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D056661:Human bocavirus / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008097:Lithuania; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013048:Specimen Handling; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins
keywords: ELISA; Human bocaviruses; Seroepidemiology; Virus-like particles; Yeast