title: Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insult.
abstract: Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we examined the transcriptomic profiles of hepatic tissue in hybrid striped bass exposed to chronic hypoxia (90days at 25% oxygen saturation) and acute hypoxia (6h at 25% oxygen saturation). Using high-throughput RNA-seq, we found that over 1400 genes were differentially expressed under disparate oxygen conditions, with the vast majority of transcriptional changes occurring in the acute hypoxia treatment. Gene pathway and bioenergetics analyses revealed hypoxia-mediated perturbation of genes and gene networks related to lipid metabolism, cell death, and changes in hepatic mitochondrial content and cellular respiration. This study offers a more comprehensive view of the temporal and tissue-specific transcriptional changes that occur during hypoxia, and reveals new and shared mechanisms of hypoxia tolerance in teleosts.
authors: United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, Auburn, AL 36832, USA. Electronic address: Benjamin.Beck@ars.usda.gov.
authors: Benjamin H
authors: BH
authors: Beck
authors: United States Department of Agriculture, Agricultural Research Service, Stuttgart National Aquaculture Research Center, Stuttgart, AR 72160, USA.
authors: S Adam
authors: SA
authors: Fuller
authors: School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors: Chao
authors: C
authors: Li
authors: United States Department of Agriculture, Agricultural Research Service, Stuttgart National Aquaculture Research Center, Stuttgart, AR 72160, USA.
authors: Bartholomew W
authors: BW
authors: Green
authors: School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors: Honggang
authors: H
authors: Zhao
authors: United States Department of Agriculture, Agricultural Research Service, Stuttgart National Aquaculture Research Center, Stuttgart, AR 72160, USA.
authors: Steven D
authors: SD
authors: Rawles
authors: United States Department of Agriculture, Agricultural Research Service, Stuttgart National Aquaculture Research Center, Stuttgart, AR 72160, USA.
authors: Carl D
authors: CD
authors: Webster
authors: School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors: Eric
authors: E
authors: Peatman
mesh_terms: D000208:Acute Disease; D000818:Animals; D001492:Bass / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D002908:Chronic Disease; D004734:Energy Metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D000860:Hypoxia / Q000503:physiopathology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D058977:Molecular Sequence Annotation; D010100:Oxygen / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D059467:Transcriptome / Q000235:genetics*
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger; D010100:Oxygen
keywords: Bass; Hypoxia; Mitochondria; Morone; RNA-seq; Transcriptome