title: Implementation of an Automated High-Throughput Plasmid DNA Production Pipeline.
abstract: Biologics sample management facilities are often responsible for a diversity of large-molecule reagent types, such as DNA, RNAi, and protein libraries. Historically, the management of large molecules was dispersed into multiple laboratories. As methodologies to support pathway discovery, antibody discovery, and protein production have become high throughput, the implementation of automation and centralized inventory management tools has become important. To this end, to improve sample tracking, throughput, and accuracy, we have implemented a module-based automation system integrated into inventory management software using multiple platforms (Hamilton, Hudson, Dynamic Devices, and Brooks). Here we describe the implementation of these systems with a focus on high-throughput plasmid DNA production management.
authors: Genentech, Biologics Resource Management, South San Francisco, CA, USA billeci.karen@gene.com.
authors: Karen
authors: K
authors: Billeci
authors: PhyNexus, Inc., San Jose, CA, USA.
authors: Christopher
authors: C
authors: Suh
authors: Genentech, Biologics Resource Management, South San Francisco, CA, USA.
authors: Tina
authors: T
authors: Di Ioia
authors: Genentech, Biologics Resource Management, South San Francisco, CA, USA.
authors: Lovejit
authors: L
authors: Singh
authors: Genentech, Biologics Resource Management, South San Francisco, CA, USA.
authors: Ryan
authors: R
authors: Abraham
authors: Genentech, Biologics Resource Management, South San Francisco, CA, USA.
authors: Anne
authors: A
authors: Baldwin
authors: Genentech, Biologics Resource Management, South San Francisco, CA, USA.
authors: Stephen
authors: S
authors: Monteclaro
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D010957:Plasmids / Q000302:isolation & purification*
chemical_list: D004247:DNA
keywords: compound management; laboratory design; robotics and instrumentation; workflow