title: Transcriptome and Expression Patterns of Chemosensory Genes in Antennae of the Parasitoid Wasp Chouioia cunea.
abstract: Chouioia cunea Yang is an endoparasitic wasp that attacks pupae of Hyphantria cunea (Drury), an invasive moth species that severely damages forests in China. Chemosensory systems of insects are used to detect volatile chemical odors such as female sex pheromones and host plant volatiles. The antennae of parasite wasps are important for host detection and other sensory-mediated behaviors. We identified and documented differential expression profiles of chemoreception genes in C. cunea antennae. A total of 25 OBPs, 80 ORs, 10 IRs, 11 CSP, 1 SNMPs, and 17 GRs were annotated from adult male and female C. cunea antennal transcriptomes. The expression profiles of 25 OBPs, 16 ORs, and 17 GRs, 5 CSP, 5 IRs and 1 SNMP were determined by RT-PCR and RT-qPCR for the antenna, head, thorax, and abdomen of male and female C. cunea. A total of 8 OBPs, 14 ORs, and 8 GRs, 1 CSP, 4 IRs and 1 SNMPs were exclusively or primarily expressed in female antennae. These female antennal-specific or dominant expression profiles may assist in locating suitable host and oviposition sites. These genes will provide useful targets for advanced study of their biological functions.
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, 300387, Tianjin, China.
authors: Yanni
authors: Y
authors: Zhao
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, 300387, Tianjin, China.
authors: Fengzhu
authors: F
authors: Wang
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, 300387, Tianjin, China.
authors: Xinyue
authors: X
authors: Zhang
authors: Natural Enemy Breeding Center of Luohe Central South Forestry, 462000, Henan, China.
authors: Suhua
authors: S
authors: Zhang
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, 300387, Tianjin, China.
authors: Shilong
authors: S
authors: Guo
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, 300387, Tianjin, China.
authors: Gengping
authors: G
authors: Zhu
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, 300387, Tianjin, China.
authors: Qiang
authors: Q
authors: Liu
authors: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, 300387, Tianjin, China.
authors: Min
authors: M
authors: Li
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D019476:Insect Proteins / Q000096:biosynthesis*; D008297:Male; D012903:Smell / Q000502:physiology; D059467:Transcriptome / Q000502:physiology*; D014863:Wasps / Q000378:metabolism*
chemical_list: D019476:Insect Proteins