title: The effect and action mechanism of resveratrol on the vascular endothelial cell by high glucose treatment.
abstract: To investigate the effect and action mechanism of resveratrol on the vascular endothelial cell by high glucose treatment. Primarily cultured human umbilical vein endothelial cells (HUVECs) were pretreated by resveratrol (0.2 μmol/L) and holding for 6 h, and then cultured in Dulbecco Modified Eagle Medium (DMEM) within 0.45 mmol/L of palmimte acid and 32.8 mmol/L of glucose, which is holding for 12 h. The cells were collected to analyze the expression of E-selected element. Supernatant of cultured cells, induced by 100 nmol/L insulin for 30 min, was used to analyze the nitric oxide content. Compared with normal control cells, the secretion of nitric oxide is stimulated by insulin decrease, however, the expression of E-selected element increased in HUVEC. Resveratrol treatment increased the secretion of nitric oxide stimulated by insulin and decreased the expression of E-selected element and partly counteracts the impairment of high glucose and palmitate acid on the function of endothelial cells. Resveratrol can improve and protect the function of high glucose and fatty acid cultured endothelial cell, and therefore may be a promising medicine in the prevention or therapy of diabetic macrovascular diseases.
authors: Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China.
authors: Xun
authors: X
authors: Liu
authors: Department of Ophthalmology, Weifang People's Hospital, Weifang 261041, PR China.
authors: Jie
authors: J
authors: Tian
authors: Department of Ophthalmology, Dalian Friendship Hospital, Dalian 116001, PR China.
authors: Quanhao
authors: Q
authors: Bai
authors: Faculty of Science and Natural Resources, University Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia.
authors: Muhammad Aqeel
authors: MA
authors: Ashraf
authors: Institute of Physiology & Pharmacology, University of Agriculture, 38040 Faisalabad, Pakistan.
authors: Maliha
authors: M
authors: Sarfraz
authors: Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, PR China.
authors: Bojun
authors: B
authors: Zhao
keywords: Effect and action mechanism; High glucose; Resveratrol; Vascular endothelial cell