title: Development and evaluation of real-time PCR assays for bloodmeal identification in Culicoides midges.
abstract: Culicoides (Diptera: Ceratopogonidae) midges are the biological vectors of a number of arboviruses of veterinary importance. However, knowledge relating to the basic biology of some species, including their host-feeding preferences, is limited. Identification of host-feeding preferences in haematophagous insects can help to elucidate the transmission dynamics of the arboviruses they may transmit. In this study, a series of semi-quantitative real-time polymerase chain reaction (qPCR) assays to identify the vertebrate host sources of bloodmeals of Culicoides midges was developed. Two pan-reactive species group and seven species-specific qPCR assays were developed and evaluated. The assays are quick to perform and less expensive than nucleic acid sequencing of bloodmeals. Using these assays, it was possible to rapidly test nearly 700 blood-fed midges of various species from several geographic locations in Australia.
authors: Virology Laboratory, Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.|Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia.
authors: M R
authors: MR
authors: VAN DER Saag
authors: Virology Laboratory, Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.
authors: X
authors: X
authors: Gu
authors: Farm Animal and Veterinary Public Health, Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia.
authors: M P
authors: MP
authors: Ward
authors: Virology Laboratory, Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.
authors: P D
authors: PD
authors: Kirkland
mesh_terms: D000818:Animals; D001717:Birds; D002519:Ceratopogonidae / Q000502:physiology*; D004247:DNA / Q000032:analysis*; D004032:Diet; D005247:Feeding Behavior; D020387:Food Chain; D006801:Humans; D058751:Livestock; D009517:New South Wales; D015876:Northern Territory; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards; D012104:Reptiles; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014904:Western Australia
chemical_list: D004247:DNA
keywords: Culicoides; bloodmeal; host identification; midge; real-time PCR