title: High PTPRQ Expression and Its Relationship to Expression of PTPRZ1 and the Presence of KRAS Mutations in Colorectal Cancer Tissues.
abstract: BACKGROUND
  The risk of sporadic colorectal cancer (CRC) is strongly influenced by Iifestyle, environmental and genetic factors. Protein tyrosine phosphatases belong to a group of enzymes whose role in CRC has not yet been intensively studied. They play an important role in activation/de-activation of many enzymes, influencing cell biology by catalyzing reactions opposing those catalyzed by kinases. Protein tyrosine phosphatase receptor-like type Q (PTPRQ) and protein tyrosine phosphatase receptor-like type Z polypeptide 1 (PTPRZ1) have both been shown to be important in development of many cancer types including CRC.
  
  
  METHODS
  The expression level of PTPRQ and PTPRZ1 was determined by real-time polymerase chain reaction in 16 CRC tissues obtained from patients diagnosed with adenocarcinoma coli.
  
  
  RESULTS
  We revealed a high level of PTPRQ expression (p=0.0080), as well as an association between expression levels of PTPRQ and PTPRZ1 (p<0.0001). Moreover PTPRQ expression was higher in tissues presenting with Kirsten rat sarcoma viral oncogene homolog (KRAS) mutation (p=0.0293).
  
  
  CONCLUSIONS
  We confirmed the contribution of PTPRZ1 and especially PTPRQ in CRC development, supporting the hypothesis that PTPRQ is a candidate oncogene, playing a crucial role in phosphorylation/dephosphorylation signaling pathways.
authors: Genetics Department, Wroclaw Medical University, Wroclaw, Poland izabela.laczmanska@umed.wroc.pl.
authors: Izabela
authors: I
authors: Laczmanska
authors: Genetics Department, Wroclaw Medical University, Wroclaw, Poland.
authors: Pawel
authors: P
authors: Karpinski
authors: Genetics Department, Wroclaw Medical University, Wroclaw, Poland.
authors: Justyna
authors: J
authors: Gil
authors: Department of Endocrinology and Diabetology, Wroclaw Medical University, Wroclaw, Poland.
authors: Lukasz
authors: L
authors: Laczmanski
authors: First Department of Surgical Oncology, Lower Silesian Oncology Center, Wroclaw, Poland.
authors: Marek
authors: M
authors: Bebenek
authors: Genetics Department, Wroclaw Medical University, Wroclaw, Poland.
authors: Maria M
authors: MM
authors: Sasiadek
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010641:Phenotype; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3 / Q000235:genetics*; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D014408:Biomarkers, Tumor; C117307:KRAS protein, human; C516917:PTPRQ protein, human; C516895:PTPRZ1 protein, human; D054631:Receptor-Like Protein Tyrosine Phosphatases, Class 3; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D016283:Proto-Oncogene Proteins p21(ras)
keywords: PTPRQ; PTPRZ1; Protein tyrosine phosphatase; colorectal cancer