title: Quantitative proteomic study of human prostate cancer cells with different metastatic potentials.
abstract: Metastatic dissemination is a feature of most cancers including prostate cancer (PCa), and is the main cause of treatment failure and mortality. The aim of the study is to explore the mechanisms of PCa metastasis and to search for potential prognostic markers using proteomics. Two-dimensional fluorescent differential gel electrophoresis (2D-DIGE) was used to quantify proteins in normal prostate epithelial cells, bone metastasis-derived PC-3 cells, and visceral metastasis-derived PC-3M cells. Metastatic potential was confirmed by flow cytometry, electron microscopy, proliferating cell nuclear antigen assay, and wound healing assay. Differential protein expression was compared between PCa cells with different metastatic potentials (LNcap, DU145, PC-3 and PC-3M) and normal prostate epithelial cells (RWPE-1). Selected candidate proteins in human prostate tissues were analyzed using GOA, UniProt and GeneCards analyses. Eighty-six proteins were differentially expressed between cell lines (>1.5-fold, P<0.05). Among them, twelve proteins were identified by MALDI-TOF-MS. One protein was upregulated in normal prostate epithelial cells, nine proteins were upregulated in PC-3, and two proteins were upregulated in PC-3M. Proteins were divided into five groups according to their functions. The SETDB1 protein was closely associated with the prognosis of PCa. Bioinformatics suggested that SETDB1 might promote PCa bone metastasis through the WNT pathway. In conclusion, SETDB1 might be associated with the development of bone metastases from PCa. Further study is necessary to assess its exact role in PCa.
authors: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors: Qun
authors: Q
authors: Li
authors: The Key Laboratory of Pathobiology, Ministry of Education, Bethune Medical School, Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Yilei
authors: Y
authors: Li
authors: Department of Endoscopy in Special Clinic Room, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors: Yanying
authors: Y
authors: Wang
authors: Department of Ultrasound, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors: Zheng
authors: Z
authors: Cui
authors: The Hepatobiliary Pancreatic Surgery, Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China.
authors: Lulu
authors: L
authors: Gong
authors: The Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Zhigang
authors: Z
authors: Qu
authors: Department of Pathology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Yanping
authors: Y
authors: Zhong
authors: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors: Jun
authors: J
authors: Zhou
authors: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors: Ying
authors: Y
authors: Zhou
authors: Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
authors: Yong
authors: Y
authors: Gao
authors: The Key Laboratory of Pathobiology, Ministry of Education, Bethune Medical School, Jilin University, Changchun, Jilin 130021, P.R. China.
authors: Yulin
authors: Y
authors: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011496:Protein Methyltransferases / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C454062:SETDB1 protein, human