title: The agar diffusion scratch assay--A novel method to assess the bioactive and cytotoxic potential of new materials and compounds.
abstract: A profound in vitro evaluation not only of the cytotoxic but also of bioactive potential of a given compound or material is crucial for predicting potential effects in the in vivo situation. However, most of the current methods have weaknesses in either the quantitative or qualitative assessment of cytotoxicity and/or bioactivity of the test compound. Here we describe a novel assay combining the ISO 10993-5 agar diffusion test and the scratch also termed wound healing assay. In contrast to these original tests this assay is able to detect and distinguish between cytotoxic, cell migration modifying and cytotoxic plus cell migration modifying compounds, and this at higher sensitivity and in a quantitative way.
authors: Institute of Life Technologies, HES-SO Valais-Wallis, CH-1950 Sion, Switzerland.
authors: Mascha
authors: M
authors: Pusnik
authors: Institute of Life Technologies, HES-SO Valais-Wallis, CH-1950 Sion, Switzerland.
authors: Minire
authors: M
authors: Imeri
authors: Institute of Life Technologies, HES-SO Valais-Wallis, CH-1950 Sion, Switzerland.
authors: Grégoire
authors: G
authors: Deppierraz
authors: Laboratory for Biointerfaces, Swiss Federal Laboratories for Materials Science and Technology - Empa, CH-9014 St. Gallen, Switzerland.
authors: Arie
authors: A
authors: Bruinink
authors: Institute of Life Technologies, HES-SO Valais-Wallis, CH-1950 Sion, Switzerland.
authors: Manfred
authors: M
authors: Zinn
mesh_terms: D000362:Agar; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D051379:Mice
chemical_list: D000362:Agar