title: SERCA2a upregulation ameliorates cellular alternans induced by metabolic inhibition.
abstract: Cardiac alternans has been associated with the incidence of ventricular tachyarrhythmias and sudden cardiac death. The aim of this study was to investigate the effect of impaired mitochondrial function in the genesis of cellular alternans and to examine whether modulating the sarcoplasmic reticulum (SR) Ca(2+)ameliorates the level of alternans. Cardiomyocytes isolated from control and doxycyline-induced sarco(endo)plasmic reticulum Ca(2+)-ATPase 2a (SERCA2a)-upregulated mice were loaded with two different Ca(2+)indicators to selectively measure mitochondrial and cytosolic Ca(2+)using a custom-made fluorescence photometry system. The degree of alternans was defined as the alternans ratio (AR) [1 - (small Ca(2+)intensity)/(large Ca(2+)intensity)]. Blocking of complex I and II, cytochrome-coxidase, F0F1synthase, α-ketoglutarate dehydrogenase of the electron transport chain, increased alternans in both control and SERCA2a mice (P< 0.01). Changes in AR in SERCA2a-upregulated mice were significantly less pronounced than those observed in control in seven of nine tested conditions (P< 0.04).N-acetyl-l-cysteine (NAC), rescued alternans in myocytes that were previously exposed to an oxidizing agent (P< 0.001). CGP, an antagonist of the mitochondrial Na(+)-Ca(2+)exchanger, had the most severe effect on AR. Exposure to cyclosporin A, a blocker of the mitochondrial permeability transition pore reduced CGP-induced alternans (P< 0.0001). The major findings of this study are that impairment of mitochondrial Ca(2+)cycling and energy production leads to a higher amplitude of alternans in both control and SERCA2a-upregulated mice, but changes in SERCA2a-upregulated mice are less severe, indicating that SERCA2a mice are more capable of sustaining electrical stability during stress. This suggests a relationship between sarcoplasmic Ca(2+)content and mitochondrial dysfunction during alternans, which may potentially help to understand changes in Ca(2+)signaling in myocytes from diseased hearts, leading to new therapeutic targets.
authors: Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts; Department of Cardiology and Pulmonology, Charité-Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany; and.
authors: Victoria
authors: V
authors: Stary
authors: Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts;
authors: Dheeraj
authors: D
authors: Puppala
authors: Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts;
authors: Marielle
authors: M
authors: Scherrer-Crosbie
authors: Department of Medicine, University of California San Diego, La Jolla, California.
authors: Wolfgang H
authors: WH
authors: Dillmann
authors: Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts; aarmoundas@partners.org.
authors: Antonis A
authors: AA
authors: Armoundas
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010539:Permeability / Q000187:drug effects; D012519:Sarcoplasmic Reticulum / Q000378:metabolism* / Q000502:physiology*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D019831:Sodium-Calcium Exchanger / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D019831:Sodium-Calcium Exchanger; D016572:Cyclosporine; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium
keywords: EC coupling; SERCA; arrhythmias; calcium; cardiac alternans; electrophysiological; heart failure; mitochondria; molecular