title: Modeling Growth Kinetic Parameters of Salmonella Enteritidis SE86 on Homemade Mayonnaise Under Isothermal and Nonisothermal Conditions.
abstract: During the last decade, a specific strain of Salmonella Enteritidis (named SE86) has been identified as the major etiological agent responsible for salmonellosis in the State of Rio Grande do Sul, Southern Brazil, and the main food vehicle was homemade mayonnaise (HM). This study aimed to model the growth prediction of SE86 on HM under isothermal and nonisothermal conditions. SE86 was inoculated on HM and stored at 7, 10, 15, 20, 25, 30, and 37°C. Growth curves were built by fitting data to the Baranyi's DMFit, generating r(2) values greater than 0.98 for primary models. Secondary model was fitted with Ratkowsky equation, generating r(2) and root mean square error values of 0.99 and 0.016, respectively. Also, the growth of SE86 under nonisothermal conditions simulating abuse temperature during preparation, storage, and serving of HM was studied. Experimental data showed that SE86 did not grow on HM at 7°C for 30 days. At 10°C, no growth was observed until approximately 18 h, and the infective dose (assumed as 10(6) CFU/g) was reached after 8.1 days. However, the same numbers of SE86 were attained after 6 hours at 37°C. Experimental data demonstrated shorter lag times than those generated by ComBase Predictive Models, suggesting that SE86 is very well adapted for growing on HM. SE86 stored under nonisothermal conditions increased population to reach about 10(6) CFU/g after approximately 30 hours of storage. In conclusion, the developed model can be used to predict the growth of SE86 on HM under various temperatures, and considering this pathogen, HM can be produced if safe eggs are used and HM is stored below 7°C.
authors: 1 Department of Food Sciences, Institute of Food Science and Technology, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre, Rio Grande do Sul, Brazil .
authors: Susana de Oliveira
authors: Sde O
authors: Elias
authors: 2 Department of Food Science, Faculty of Food Engineering, University of Campinas , (UNICAMP) Campinas, São Paulo, Brazil .
authors: Verônica Ortiz
authors: VO
authors: Alvarenga
authors: 3 Federal University of Parana (UFPR) , Campus Jandaia do Sul, Jandaia do Sul, Paraná, Brazil .
authors: Daniel Angelo
authors: DA
authors: Longhi
authors: 2 Department of Food Science, Faculty of Food Engineering, University of Campinas , (UNICAMP) Campinas, São Paulo, Brazil .
authors: Anderson de Souza
authors: Ade S
authors: Sant'Ana
authors: 1 Department of Food Sciences, Institute of Food Science and Technology, Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre, Rio Grande do Sul, Brazil .
authors: Eduardo Cesar
authors: EC
authors: Tondo
mesh_terms: D001938:Brazil; D003212:Condiments / Q000382:microbiology; D003470:Culture Media; D004196:Disease Outbreaks; D004531:Eggs / Q000382:microbiology*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D012478:Salmonella Food Poisoning / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000254:growth & development; D011198:Solanum tuberosum / Q000382:microbiology
chemical_list: D003470:Culture Media