title: Chaperonin GroEL/GroES Over-Expression Promotes Aminoglycoside Resistance and Reduces Drug Susceptibilities in Escherichia coli Following Exposure to Sublethal Aminoglycoside Doses.
abstract: Antibiotic resistance is an increasing challenge to modern healthcare. Aminoglycoside antibiotics cause translation corruption and protein misfolding and aggregation in Escherichia coli. We previously showed that chaperonin GroEL/GroES depletion and over-expression sensitize and promote short-term tolerance, respectively, to this drug class. Here, we show that chaperonin GroEL/GroES over-expression accelerates acquisition of streptomycin resistance and reduces susceptibility to several other antibiotics following sub-lethal streptomycin antibiotic exposure. Chaperonin buffering could provide a novel mechanism for emergence of antibiotic resistance.
authors: Department of Cellular and Molecular Medicine, University of Copenhagen Copenhagen, Denmark.
authors: Lise
authors: L
authors: Goltermann
authors: Department of Cellular and Molecular Medicine, University of Copenhagen Copenhagen, Denmark.
authors: Menachem V
authors: MV
authors: Sarusie
authors: Department of Cellular and Molecular Medicine, University of Copenhagen Copenhagen, Denmark.
authors: Thomas
authors: T
authors: Bentin
keywords: aminoglycosides; antibiotic resistance; chaperonin; mRNA translation; protein misfolding