title: [Expressions of Notch3, Notch4, Frizzled2 and Tead1 in rats with focal cerebral ischemia-reperfusion].
abstract: OBJECTIVE
  To investigate the expressions of Notch3, Notch4, Frizzled2 and Tead1 at protein levels in the cortex of frontal and parietal lobes after focal cerebral ischemia-reperfusion (I/R) in rats.
  
  
  METHODS
  The focal cerebral I/R model was established by intraluminal thread occlusion of the middle cerebral artery (MCAO). The animals were divided into sham operation group (sham group) and I/R group. The infarct area of the brain was measured by 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining technique after 1 week. Protein expression levels of Notch3 and Frizzled2 in the cortex of frontal and parietal lobes were detected by immunohistochemical staining, and protein expression levels of Notch4 and Tead1 were detected by Western blot analysis.
  
  
  RESULTS
  The infarct area of rats in I/R group was very obvious. The protein expressions of Notch3 and Frizzled2 in I/R group were higher than those in sham group (P<0.05, P<0.01). The protein expression of Notch4 in I/R group was significantly higher than that in sham group (P<0.01), but the protein expression level of Tead1 in I/R group was significantly lower than that in sham group(P<0.01).
  
  
  CONCLUSIONS
  Notch signal transduction pathway was activated after focal cerebral ischemia/reperfusion in MCAO rats.
authors: Clinical College of Traditional Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230038, China; Email: zhhm050518@126.com.
authors: Hongmei
authors: H
authors: Zhang
authors: Jianpeng
authors: J
authors: Hu
authors: Jian
authors: J
authors: Wang
authors: Xuncui
authors: X
authors: Wang
authors: Wei
authors: W
authors: Xu
authors: Hui
authors: H
authors: Tan
authors: Lina
authors: L
authors: Wang
authors: Lei
authors: L
authors: Lü
authors: Anhui
authors: A
authors: Wen
mesh_terms: D000818:Animals; D001921:Brain; D002545:Brain Ischemia; D002544:Cerebral Infarction; D004268:DNA-Binding Proteins; D051157:Frizzled Receptors; D051381:Rats; D000071656:Receptor, Notch3; D000074562:Receptor, Notch4; D051880:Receptors, Notch; D015424:Reperfusion; D015427:Reperfusion Injury; D015398:Signal Transduction; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D051157:Frizzled Receptors; C498556:Notch3 protein, rat; C560417:Notch4 protein, rat; D000071656:Receptor, Notch3; D000074562:Receptor, Notch4; D051880:Receptors, Notch; D014157:Transcription Factors