title: Exercise-induced oxidatively damaged DNA in humans: evaluation in plasma or urine?
abstract: Physical exercise can induce oxidative damage in humans. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a widely known biomarker of DNA oxidation, which can be determined in blood and urine. The aim of the present study was to compare these two biological fluids in terms of which is more suitable for the estimation of the oxidative damage of DNA by measuring the concentration of 8-OHdG one hour after maximal exercise by enzyme immunoassay. The concentration of 8-OHdG increased with exercise only in plasma (p < 0.001), and values differed between exercise tests in both plasma and urine (p < 0.05). In conclusion, plasma appears to be more sensitive to exercise-induced 8-OHdG changes than urine and, hence, a more appropriate medium for assessing oxidative damage of DNA, although the poor repeatability of the measurement needs to be addressed in future studies.
authors: a School of Physical Education and Sport Science, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors: Christina
authors: C
authors: Karpouzi
authors: a School of Physical Education and Sport Science, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors: Stefanos
authors: S
authors: Nikolaidis
authors: a School of Physical Education and Sport Science, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors: Athanasios
authors: A
authors: Kabasakalis
authors: a School of Physical Education and Sport Science, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors: George
authors: G
authors: Tsalis
authors: a School of Physical Education and Sport Science, Aristotle University of Thessaloniki , Thessaloniki , Greece.
authors: Vassilis
authors: V
authors: Mougios
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D015415:Biomarkers / Q000097:blood* / Q000652:urine; D004247:DNA / Q000097:blood / Q000652:urine; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood / Q000652:urine; D015444:Exercise / Q000502:physiology; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D013550:Swimming
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine
keywords: 8-Hydroxy-2′-deoxyguanosine; maximal exercise; oxidative stress; swimming