title: Transcriptome Analysis of an Anthracnose-Resistant Tea Plant Cultivar Reveals Genes Associated with Resistance to Colletotrichum camelliae.
abstract: Tea plant breeding is a topic of great economic importance. However, disease remains a major cause of yield and quality losses. In this study, an anthracnose-resistant cultivar, ZC108, was developed. An infection assay revealed different responses to Colletotrichum sp. infection between ZC108 and its parent cultivar LJ43. ZC108 had greater resistance than LJ43 to Colletotrichum camelliae. Additionally, ZC108 exhibited earlier sprouting in the spring, as well as different leaf shape and plant architecture. Microarray data revealed that the genes that are differentially expressed between LJ43 and ZC108 mapped to secondary metabolism-related pathways, including phenylpropanoid biosynthesis, phenylalanine metabolism, and flavonoid biosynthesis pathways. In addition, genes involved in plant hormone biosynthesis and signaling as well as plant-pathogen interaction pathways were also changed. Quantitative real-time PCR was used to examine the expression of 27 selected genes in infected and uninfected tea plant leaves. Genes encoding a MADS-box transcription factor, NBS-LRR disease-resistance protein, and phenylpropanoid metabolism pathway components (CAD, CCR, POD, beta-glucosidase, ALDH and PAL) were among those differentially expressed in ZC108.
authors: National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.|Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, China.
authors: Lu
authors: L
authors: Wang
authors: National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.|College of Horticulture, Northwest Agriculture and Forestry University, Yangling, 712100, Shaanxi, China.
authors: Yuchun
authors: Y
authors: Wang
authors: National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.
authors: Hongli
authors: H
authors: Cao
authors: National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.|Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, China.
authors: Xinyuan
authors: X
authors: Hao
authors: National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.|Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, China.
authors: Jianming
authors: J
authors: Zeng
authors: National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.|Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, China.
authors: Yajun
authors: Y
authors: Yang
authors: National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.|Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, China.
authors: Xinchao
authors: X
authors: Wang
mesh_terms: D028241:Camellia sinensis / Q000235:genetics* / Q000382:microbiology* / Q000502:physiology; D020231:Colletotrichum / Q000472:pathogenicity*; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins