title: Prediction of anticancer peptides against MCF-7 breast cancer cells from the peptidomes of Achatina fulica mucus fractions.
abstract: Several reports have shown antimicrobial and anticancer activities of mucous glycoproteins extracted from the giant African snail Achatina fulica. Anticancer properties of the snail mucous peptides remain incompletely revealed. The aim of this study was to predict anticancer peptides from A. fulica mucus. Two of HPLC-separated mucous fractions (F2 and F5) showed in vitro cytotoxicity against the breast cancer cell line (MCF-7) and normal epithelium cell line (Vero). According to the mass spectrometric analysis, 404 and 424 peptides from the F2 and F5 fractions were identified. Our comprehensive bioinformatics workflow predicted 16 putative cationic and amphipathic anticancer peptides with diverse structures from these two peptidome data. These peptides would be promising molecules for new anti-breast cancer drug development.
authors: Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors: Teerasak
authors: T
authors: E-Kobon
authors: Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors: Pennapa
authors: P
authors: Thongararm
authors: National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathum Thani 12120, Thailand.
authors: Sittiruk
authors: S
authors: Roytrakul
authors: Faculty of Dentistry, Thammasat University, Pathum Thani 12120, Thailand.
authors: Ladda
authors: L
authors: Meesuk
authors: Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
authors: Pramote
authors: P
authors: Chumnanpuen
keywords: Achatina fulica; Bioinformatics prediction; Breast cancer; Cytotoxic peptides; Peptidomics; Snail mucus