title: Intrinsic characteristics of Min proteins on the cell division of Helicobacter pylori.
abstract: Helicobacter pylori divides in the human stomach resulting in persistent infections and causing various disorders. Bacterial cell division is precisely coordinated by many molecules, including FtsZ and Min proteins. However, the role of Min proteins in H. pylori division is poorly understood. We investigated the functional characteristics of Min proteins in wild-type HPK5 and five HPK5-derivative mutants using morphological and genetic approaches. All mutants showed a filamentous shape. However, the bacterial cell growth and viability of three single-gene mutants (minC, minD, minE) were similar to that of the wild-type. The coccoid form number was lowest in the minE-disruptant, indicating that MinE contributes to the coccoid form conversion during the stationary phase. Immunofluorescence microscopic observations showed that FtsZ was dispersedly distributed throughout the bacterial cell irrespective of nucleoid position in only minD-disruptants, indicating that MinD is involved in the nucleoid occlusion system. A chase assay demonstrated that MinC loss suppressed FtsZ-degradation, indicating that FtsZ degrades in a MinC-dependent manner. Molecular interactions between FtsZ and Min proteins were confirmed by immunoprecipitation (IP)-western blotting (WB), suggesting the functional cooperation of these molecules during bacterial cell division. This study describes the intrinsic characteristics of Min proteins and provides new insights into H. pylori cell division.
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan.
authors: Yoshie
authors: Y
authors: Nishida
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan htake@kochi-u.ac.jp.
authors: Hiroaki
authors: H
authors: Takeuchi
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan.
authors: Norihito
authors: N
authors: Morimoto
authors: Department of Clinical Laboratory Medicine, Yamaguchi University, 1-1-1 MinamiKogushi, Ube-city, Yamaguchi 755-8505, Japan.
authors: Akiko
authors: A
authors: Umeda
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan.
authors: Yoshu
authors: Y
authors: Kadota
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan.
authors: Mizuki
authors: M
authors: Kira
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan.
authors: Ami
authors: A
authors: Okazaki
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan.
authors: Yoshihisa
authors: Y
authors: Matsumura
authors: Department of Clinical Laboratory Medicine, Kochi Medical School, Kohasu, Oko-cho, Nankoku-city, Kochi 783-8505, Japan.
authors: Tetsuro
authors: T
authors: Sugiura
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002455:Cell Division; D002876:Chromosomes, Bacterial; D003598:Cytoskeletal Proteins / Q000378:metabolism; D016480:Helicobacter pylori / Q000502:physiology* / Q000648:ultrastructure; D050296:Microbial Viability / Q000235:genetics; D008856:Microscopy, Fluorescence; D009154:Mutation; D011485:Protein Binding; D021381:Protein Transport; D059748:Proteolysis
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria
keywords: FtsZ; Helicobacter pylori; MinCDE; cell division