title: PCR-Based Detection of DNA Copy Number Variation.
abstract: Copy number variations are important polymorphisms that can influence gene expression within and close to the rearranged region, and results in phenotypic variation. Techniques that detect abnormalities in DNA copy number are therefore useful for studying the associations between DNA aberrations and disease phenotype and for locating critical genes. PCR-based detection of copy number of target gene using TaqMan copy number assay offers a reliable method to measure copy number variation in human genome.
authors: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Unit 72, 1515 Holcombe Blvd, Houston, TX, 77030, USA. mmehrotra@mdanderson.org.
authors: Meenakshi
authors: M
authors: Mehrotra
mesh_terms: D019295:Computational Biology / Q000379:methods; D056915:DNA Copy Number Variations; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
keywords: Array complete genomic hybridization (aCGH); Copy number variation (CNV); Copycaller software; PCR; TaqMan copy number assay