title: A characterization of grapevine of GRAS domain transcription factor gene family.
abstract: GRAS domain genes are a group of important plant-specific transcription factors that have been reported to be involved in plant development. In order to know the roles of GRAS genes in grapevine, a widely cultivated fruit crop, the study on grapevine GRAS (VvGRAS) was carried out, and from which, 43 were identified from 12× assemble grapevine genomic sequences. Further, the genomic structures, synteny, phylogeny, expression profiles in different tissues of these genes, and their roles in response to stress were investigated. Among the genes, two potential target genes (VvSCL15 and VvSCL22) for VvmiR171 were experimentally verified by PPM-RACE and RLM-RACE, in that not only the cleavage sites of miR171 on the target mRNA were mapped but also the cleaved fragments and their expressing patterns were detected. Transgenic Arabidopsis plants over expression VvSCL15 showed lower tolerance to drought and salt treatments.
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors: Xin
authors: X
authors: Sun
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.|Department of Agronomy and Horticulture, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, 212400, Jiangsu Province, People's Republic of China.
authors: Zhengqiang
authors: Z
authors: Xie
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors: Cheng
authors: C
authors: Zhang
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors: Qian
authors: Q
authors: Mu
authors: Institute of Horticulture, Jiangsu Academy of Agriculture Sciences, Nanjing, 210014, Jiangsu Province, People's Republic of China.
authors: Weimin
authors: W
authors: Wu
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China.
authors: Baoju
authors: B
authors: Wang
authors: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People's Republic of China. fanggg@njau.edu.cn.
authors: Jinggui
authors: J
authors: Fang
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D005810:Multigene Family / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D027843:Vitis / Q000235:genetics* / Q000254:growth & development
chemical_list: D014157:Transcription Factors
keywords: Expression; Function; GRAS gene family; Grapevine