title: Remote limb ischemic post-conditioning attenuates ischemia-reperfusion injury in rat skin flapby limiting oxidative stress.
abstract: OBJECTIVE
  To investigate the effect of remote ischemic post-conditioning (RIPoC) against ischemia-reperfusion (I/R) injury on flaps of rats.
  
  
  METHODS
  Sprague-Dawley rats were randomized into the Sham, Control, RIPoC1 and RIPoC2 groups. All the animals were submitted to a 5×4 cm superficial inferior epigastric artery flap. Eight hours of flap ischemia was induced and two protocols of limb RIPoC were applied. Tissue MDA level and SOD activity in 24-h reperfusion were assessed. Flap survival was assessed 7 days postoperatively.
  
  
  RESULTS
  Compared to the Control group, the RIPoC1 group showed statistically decreased MDA level at 6-, 12-, and 24-h reperfusion (P = 0.01, P < 0.01 and P < 0.01, respectively), and statistically increased SOD activity at 12- and 24-h reperfusion (P < 0.05 and P < 0.01, respectively). Flap survival rate on the 7th day was significantly higher in the RIPoC1 group than the control group (47.9 ± 6.4 vs . 29.4 ± 7.1 %, P < 0.01).
  
  
  CONCLUSIONS
  Three cycles of 5-min Limb remote ischemic post-conditioning rather than a single cycle of 15-min limb RIPoC has protective effect on flaps against ischemia-reperfusion injury by attenuating oxidative stress.
authors: School of Medicine, Shanghai Jiao Tong University, China.
authors: Yi
authors: Y
authors: Zhang
authors: School of Medicine, Shanghai Jiao Tong University, China.
authors: Hua
authors: H
authors: Xu
authors: School of Medicine, Shanghai Jiao Tong University, China.
authors: Tao
authors: T
authors: Wang
authors: School of Medicine, Shanghai Jiao Tong University, China.
authors: Jinguang
authors: J
authors: He
authors: School of Medicine, Shanghai Jiao Tong University, China.
authors: Jiao
authors: J
authors: Wei
authors: School of Medicine, Shanghai Jiao Tong University, China.
authors: Tingliang
authors: T
authors: Wang
authors: School of Medicine, Shanghai Jiao Tong University, China.
authors: Jiasheng
authors: J
authors: Dong
mesh_terms: D000818:Animals; D062109:Dermatologic Surgical Procedures / Q000379:methods; D019074:Epigastric Arteries / Q000601:surgery; D005121:Extremities / Q000293:injuries; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009336:Necrosis; D018384:Oxidative Stress; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000628:therapy*; D013482:Superoxide Dismutase / Q000032:analysis; D013524:Surgical Flaps / Q000098:blood supply* / Q000473:pathology; D013997:Time Factors
chemical_list: D008315:Malondialdehyde; D013482:Superoxide Dismutase