title: Protective Role of Adipose-Derived Stem Cells in Staphylococcus aureus-Induced Lung Injury is Mediated by RegIIIγ Secretion.
abstract: Effective and specific therapeutic approaches are still needed for treating acute lung injury caused by severe pneumonia. Adipose-derived stem cells (ADSCs) are well-characterized adult stem cells that have antibacterial and anti-inflammatory effects. In this study, we evaluated the therapeutic effect of ADSCs on Staphylococcus aureus-induced acute lung injury in mice. Our results showed that intratracheal injection of ADSCs could attenuate the severity of lung inflammation, and reduce the bacterial load as well as mortality among infected mice. Our experiments also revealed that the secretion of regenerating islet-derived IIIγ (RegIIIγ) is responsible for the protective effect of ADSCs. Moreover, the expression of RegIIIγ requires TLR2, MyD88, and JAK2/STAT3 activation. In conclusion, ADSCs exhibit a direct antimicrobial activity that is mediated primarily by the TLR2-MyD88-JAK2/STAT3-dependent secretion of RegIIIγ. Stem Cells 2016;34:1947-1956.
authors: Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
authors: Jing
authors: J
authors: Qian
authors: Department of Respiratory Medicine, Zhejiang University School of Medicine, Hangzhou, China.|Department of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, China.
authors: Yue
authors: Y
authors: Hu
authors: Department of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, China.
authors: Lifang
authors: L
authors: Zhao
authors: Department of Radiation Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors: Jingyan
authors: J
authors: Xia
authors: Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, China.
authors: Changwei
authors: C
authors: Li
authors: Division of Immunology, Hangzhou Normal University, Hangzhou, China.
authors: Liyun
authors: L
authors: Shi
authors: Department of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, China.
authors: Feng
authors: F
authors: Xu
mesh_terms: D055371:Acute Lung Injury / Q000382:microbiology* / Q000628:therapy*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D000073718:Pancreatitis-Associated Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D013203:Staphylococcal Infections / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D000890:Anti-Infective Agents; D000073718:Pancreatitis-Associated Proteins; D011994:Recombinant Proteins; C490255:Reg3g protein, mouse
keywords: Acute lung injury; Adipose-derived stem cells; Regenerating islet-derived IIIγ; Signaling pathways; Staphylococcus aureus