title: Inter-Individual Differences in RNA Levels in Human Peripheral Blood.
abstract: Relatively little is known about the range of RNA levels in human blood. This report provides assessment of peripheral blood RNA level and its inter-individual differences in a group of 35 healthy humans consisting of 25 females and 10 males ranging in age from 50 to 89 years. In this group, the average total RNA level was 14.59 μg/ml of blood, with no statistically significant difference between females and males. The individual RNA level ranged from 6.7 to 22.7 μg/ml of blood. In healthy subjects, the repeated sampling of an individual's blood showed that RNA level, whether high or low, was stable. The inter-individual differences in RNA level in blood can be attributed to both, differences in cell number and the amount of RNA per cell. The 3.4-fold range of inter-individual differences in total RNA levels, documented herein, should be taken into account when evaluating the results of quantitative RT-PCR and/or RNA sequencing studies of human blood. Based on the presented results, a comprehensive assessment of gene expression in blood should involve determination of both the amount of mRNA per unit of total RNA (U / ng RNA) and the amount of mRNA per unit of blood (U / ml blood) to assure a thorough interpretation of physiological or pathological relevance of study results.
authors: Molecular Research Center, Inc. Cincinnati, OH, United States of America.
authors: Piotr
authors: P
authors: Chomczynski
authors: Molecular Research Center, Inc. Cincinnati, OH, United States of America.
authors: William W
authors: WW
authors: Wilfinger
authors: University of Cincinnati, College of Medicine, Cincinnati, OH, United States of America.
authors: Hamid R
authors: HR
authors: Eghbalnia
authors: Molecular Research Center, Inc. Cincinnati, OH, United States of America.
authors: Amy
authors: A
authors: Kennedy
authors: Molecular Research Center, Inc. Cincinnati, OH, United States of America.
authors: Michal
authors: M
authors: Rymaszewski
authors: Molecular Research Center, Inc. Cincinnati, OH, United States of America.
authors: Karol
authors: K
authors: Mackey
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001772:Blood Cell Count; D004247:DNA / Q000097:blood; D005260:Female; D015870:Gene Expression; D020043:Genes, Essential; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000592:standards*; D012313:RNA / Q000097:blood*; D012333:RNA, Messenger / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D012333:RNA, Messenger; D012313:RNA; D004247:DNA