title: 'Pre-prosthetic use of poly(lactic-co-glycolic acid) membranes treated with oxygen plasma and TiO2 nanocomposite particles for guided bone regeneration processes'.
abstract: OBJECTIVE
  Guided bone regeneration (GBR) processes are frequently necessary to achieve appropriate substrates before the restoration of edentulous areas. This study aimed to evaluate the bone regeneration reliability of a new poly-lactic-co-glycolic acid (PLGA) membrane after treatment with oxygen plasma (PO2) and titanium dioxide (TiO2) composite nanoparticles.
  
  
  METHODS
  Circumferential bone defects (diameter: 10mm; depth: 3mm) were created on the parietal bones of eight experimentation rabbits and were randomly covered with control membranes (Group 1: PLGA) or experimental membranes (Group 2: PLGA/PO2/TiO2). The animals were euthanized two months afterwards, and a morphologic study was then performed under microscope using ROI (region of interest) colour analysis. Percentage of new bone formation, length of mineralised bone formed in the grown defects, concentration of osteoclasts, and intensity of osteosynthetic activity were assessed. Comparisons among the groups and with the original bone tissue were made using the Kruskal-Wallis test. The level of significance was set in advance at a=0.05.
  
  
  RESULTS
  The experimental group recorded higher values for new bone formation, mineralised bone length, and osteoclast concentration; this group also registered the highest osteosynthetic activity. Bone layers in advanced formation stages and low proportions of immature tissue were observed in the study group.
  
  
  CONCLUSIONS
  The functionalised membranes showed the best efficacy for bone regeneration.
  
  
  CONCLUSIONS
  The addition of TiO2 nanoparticles onto PLGA/PO2 membranes for GBR processes may be a promising technique to restore bone dimensions and anatomic contours as a prerequisite to well-supported and natural-appearing prosthetic rehabilitations.
authors: Department of Stomatology, Faculty of Dentistry, University of Seville (US), C/Avicena, s/n, 41009 Seville, Spain.
authors: Gabriel
authors: G
authors: Castillo-Dalí
authors: Department of Buccofacial Prostheses, Faculty of Dentistry, Complutense University of Madrid (UCM), Pza. Ramón y Cajal, s/n, 28040 Madrid, Spain. Electronic address: raquel.castillo@ucm.es.
authors: Raquel
authors: R
authors: Castillo-Oyagüe
authors: Institute of Materials Sciences, Advanced Center of Scientific Research (CSIC), Avda. Américo Vespuccio, no. 49, Isla de la Cartuja, 41092 Seville, Spain.
authors: Antonia
authors: A
authors: Terriza
authors: Faculté de Chirurgie Dentaire, Université Paris V- Descartes, rue Maurice Arnoux, no. 1, 92120 Montrouge, Paris, France.
authors: Jean-Louis
authors: JL
authors: Saffar
authors: Faculté de Chirurgie Dentaire, Université Paris V- Descartes, rue Maurice Arnoux, no. 1, 92120 Montrouge, Paris, France.
authors: Antonio
authors: A
authors: Batista-Cruzado
authors: School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, CF14 4XY, Cardiff, Wales, UK.
authors: Christopher D
authors: CD
authors: Lynch
authors: School of Dentistry, College of Biomedical and Life Sciences, Cardiff University, CF14 4XY, Cardiff, Wales, UK.
authors: Alastair J
authors: AJ
authors: Sloan
authors: Department of Stomatology, Faculty of Dentistry, University of Seville (US), C/Avicena, s/n, 41009 Seville, Spain.
authors: José-Luis
authors: JL
authors: Gutiérrez-Pérez
authors: Department of Stomatology, Faculty of Dentistry, University of Seville (US), C/Avicena, s/n, 41009 Seville, Spain.
authors: Daniel
authors: D
authors: Torres-Lagares
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000601:surgery; D001861:Bone Regeneration / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods*; D019344:Lactic Acid / Q000494:pharmacology*; D008567:Membranes, Artificial; D053761:Nanocomposites / Q000737:chemistry*; D010010:Osteoclasts / Q000187:drug effects / Q000473:pathology; D010100:Oxygen / Q000737:chemistry*; D058626:Plasma Gases / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D015203:Reproducibility of Results; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D058626:Plasma Gases; C009495:titanium dioxide; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014025:Titanium; D010100:Oxygen
keywords: Guided bone regeneration (GBR); Nanocomposites; Oxygen plasma (PO(2)); PLGA barriers; Poly(lactic-co-glycolic acid) membranes; TiO(2) particles