title: Introducing Discrete Frequency Infrared Technology for High-Throughput Biofluid Screening.
abstract: Accurate early diagnosis is critical to patient survival, management and quality of life. Biofluids are key to early diagnosis due to their ease of collection and intimate involvement in human function. Large-scale mid-IR imaging of dried fluid deposits offers a high-throughput molecular analysis paradigm for the biomedical laboratory. The exciting advent of tuneable quantum cascade lasers allows for the collection of discrete frequency infrared data enabling clinically relevant timescales. By scanning targeted frequencies spectral quality, reproducibility and diagnostic potential can be maintained while significantly reducing acquisition time and processing requirements, sampling 16 serum spots with 0.6, 5.1 and 15% relative standard deviation (RSD) for 199, 14 and 9 discrete frequencies respectively. We use this reproducible methodology to show proof of concept rapid diagnostics; 40 unique dried liquid biopsies from brain, breast, lung and skin cancer patients were classified in 2.4 cumulative seconds against 10 non-cancer controls with accuracies of up to 90%.
authors: University of Manchester, School of Chemical Engineering and Analytical Science, Manchester, M13 9PL, United Kingdom.|WestCHEM, Technology and Innovation Centre, 99 George St, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, G1 1RD, UK.
authors: Caryn
authors: C
authors: Hughes
authors: WestCHEM, Technology and Innovation Centre, 99 George St, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, G1 1RD, UK.
authors: Graeme
authors: G
authors: Clemens
authors: Daylight Solutions Inc., San Diego, CA 92128, USA.
authors: Benjamin
authors: B
authors: Bird
authors: Lancashire Teaching Hospitals NHS Trust, Royal Preston Hospital, Department of Pathology, Preston, PR2 9HT, UK.
authors: Timothy
authors: T
authors: Dawson
authors: Lancashire Teaching Hospitals NHS Trust, Royal Preston Hospital, Department of Pathology, Preston, PR2 9HT, UK.
authors: Katherine M
authors: KM
authors: Ashton
authors: The Walton Centre NHS Foundation Trust, The Walton Centre for Neurology and Neurosurgery, Liverpool, L9 7LJ, UK.
authors: Michael D
authors: MD
authors: Jenkinson
authors: The Walton Centre NHS Foundation Trust, The Walton Centre for Neurology and Neurosurgery, Liverpool, L9 7LJ, UK.
authors: Andrew
authors: A
authors: Brodbelt
authors: Daylight Solutions Inc., San Diego, CA 92128, USA.
authors: Miles
authors: M
authors: Weida
authors: Daylight Solutions Inc., San Diego, CA 92128, USA.
authors: Edeline
authors: E
authors: Fotheringham
authors: Daylight Solutions Inc., San Diego, CA 92128, USA.
authors: Matthew
authors: M
authors: Barre
authors: Daylight Solutions Inc., San Diego, CA 92128, USA.
authors: Jeremy
authors: J
authors: Rowlette
authors: WestCHEM, Technology and Innovation Centre, 99 George St, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, G1 1RD, UK.
authors: Matthew J
authors: MJ
authors: Baker
mesh_terms: D001331:Automation; D001706:Biopsy; D001826:Body Fluids / Q000737:chemistry*; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D059788:Dried Blood Spot Testing / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D054023:Lasers, Semiconductor; D018613:Microscopy, Confocal; D015203:Reproducibility of Results; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods*