title: Quantitative Real-Time PCR: Recent Advances.
abstract: Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or second sequence is typically adequate. The quantification process employs melting curve analysis and/or fluorescent detection systems and can provide amplification and genotyping in a relatively short time. Here we describe the properties and uses of various fluorescent detection systems used for quantification.
authors: Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors: Charanjeet
authors: C
authors: Singh
authors: Department of Pathology, Cytopathology and Molecular Pathology, MD Anderson Cancer Center, The University ofTexas, Houston, TX, USA. sroy2@mdanderson.org.
authors: Sinchita
authors: S
authors: Roy-Chowdhuri
mesh_terms: D000818:Animals; D017931:DNA Primers; D015342:DNA Probes; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D015342:DNA Probes
keywords: Fluorescent genotyping; Hybridization probe; Intercalation probe; Minimal residual disease; Molecular beacon; Nucleic acid quantification; Polymerase chain reaction (PCR); Quantitative PCR; Real-time PCR; q-PCR