title: Bioluminescence Imaging of Colonization and Clearance Dynamics of Brucella Suis Vaccine Strain S2 in Mice and Guinea Pigs.
abstract: The goal of this study was to develop a plasmid-based lux bio-reporter for use to obtain in vivo images of Brucella suis vaccine strain 2 (B.suis S2) infection with high resolution and good definition.
  
  
  
  The pBBR-lux (pBBR1MCS-2-lxCDABE) plasmid that carries the luxCDABE operon was introduced into B. suis S2 by electroporation yielding B. suis S2-lux. The spatial and temporal transit of B. suis S2 in mice and guinea pigs was monitored by bioluminescence imaging.
  
  
  
  The plasmid pBBR-lux is stable in vivo and does not appear to impact the virulence or growth of bacteria. This sensitive luciferase reporter could represent B. suis S2 survival in real time. B. suis S2 mainly colonized the lungs, liver, spleen, and uterus in mice and guinea pigs as demonstrated by bioluminescence imaging.
  
  
  
  The plasmid-based lux bioreporter strategy can be used to obtain high resolution in vivo images of B. suis S2 infection in mice and guinea pigs.
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Xiwen
authors: X
authors: Wang
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Zhiping
authors: Z
authors: Li
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Bo
authors: B
authors: Li
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Hang
authors: H
authors: Chi
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Jiakuan
authors: J
authors: Li
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Hongchao
authors: H
authors: Fan
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Ruizhi
authors: R
authors: Yao
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Qianxue
authors: Q
authors: Li
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Xiaolin
authors: X
authors: Dong
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Man
authors: M
authors: Chen
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Han
authors: H
authors: Qu
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Yuanyuan
authors: Y
authors: Wang
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Weicun
authors: W
authors: Gao
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Yutian
authors: Y
authors: Wang
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Yu
authors: Y
authors: Sun
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China.
authors: Rui
authors: R
authors: Sun
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China. qianj1970@126.com.
authors: Jun
authors: J
authors: Qian
authors: Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China. ammszipxia@163.com.
authors: Zhiping
authors: Z
authors: Xia
mesh_terms: D000818:Animals; D058491:Bacterial Load; D002004:Brucella Vaccine / Q000276:immunology*; D041021:Brucella suis / Q000254:growth & development* / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology* / Q000382:microbiology*; D015169:Colony Count, Microbial; D005260:Female; D006168:Guinea Pigs; D021621:Imaging, Three-Dimensional; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D010537:Peritoneum / Q000382:microbiology / Q000473:pathology
chemical_list: D002004:Brucella Vaccine; D008156:Luciferases
keywords: Animal model; Bioluminescent imaging; Brucella suis S2