title: Suppression of c‑FLIPL promotes JNK activation in malignant melanoma cells.
abstract: The up‑regulation of cellular Fas‑associated death domain‑like interleukin‑1β‑converting enzyme (FLICE)‑like inhibitory protein (c‑FLIP) has been reported in various tumor types, and has been previously shown to be associated with the clinicopathological features of melanoma. To assess its potential role in cancer therapy, the present study evaluated the effects of short hairpin (sh)RNAs of different c‑FLIP isoforms on cellular proliferation and c‑Jun N‑terminal kinase (JNK) signaling. Human c‑FLIP shRNA plasmids were constructed and transfected into the A875 melanoma cell line. It was observed that c‑FLIP shRNA exhibited strong inhibitory effects against the levels of phosphorylated‑JNK and inhibited cellular proliferation in A875 cells. Thus, this indicated that c‑FLIP long form shRNA serves a specific inhibitory role in cellular proliferation through inducing the activation of the JNK pathway in A875 cells. The present study provided insight into the development of RNAi based therapies for melanoma.
authors: Department of Dermatology, Sixth People's Hospital of Zhengzhou, Zhengzhou, Henan 450015, P.R. China.
authors: Fen
authors: F
authors: Tian
authors: Department of Dermatology, Sixth People's Hospital of Zhengzhou, Zhengzhou, Henan 450015, P.R. China.
authors: Yange
authors: Y
authors: Hu
authors: Department of Dermatology, Sixth People's Hospital of Zhengzhou, Zhengzhou, Henan 450015, P.R. China.
authors: Xixi
authors: X
authors: Sun
authors: Department of Dermatology, Sixth People's Hospital of Zhengzhou, Zhengzhou, Henan 450015, P.R. China.
authors: Gaihui
authors: G
authors: Lu
authors: Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
authors: Yan
authors: Y
authors: Li
authors: Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
authors: Jing
authors: J
authors: Yang
authors: Department of Dermatology, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
authors: Juan
authors: J
authors: Tao
mesh_terms: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004789:Enzyme Activation; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D048670:MAP Kinase Kinase 4; D008545:Melanoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; C506386:CFLAR protein, human; D020033:Protein Isoforms; D034741:RNA, Small Interfering; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D048670:MAP Kinase Kinase 4