title: Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis thaliana.
abstract: Phosphoprotein/peptide enrichment is an important technique to elucidate signaling components of defense responses with mass spectrometry. Normally, proteins can be detected easily by shotgun experiments but the low abundance of phosphoproteins hinders their detection. Here, we describe a combination of prefractionation with desalting, phosphoprotein and phosphopeptide enrichment to effectively accumulate phosphorylated proteins from leaf tissue of stressed Arabidopsis plants.
authors: Department of Stress and Developmental Biology, Leibniz-Institute of Plant Biochemistry, Weinberg 3, 06120, Halle/Saale, Germany.
authors: Ines
authors: I
authors: Lassowskat
authors: Department of Stress and Developmental Biology, Leibniz-Institute of Plant Biochemistry, Weinberg 3, 06120, Halle/Saale, Germany.
authors: Wolfgang
authors: W
authors: Hoehenwarter
authors: Department of Stress and Developmental Biology, Leibniz-Institute of Plant Biochemistry, Weinberg 3, 06120, Halle/Saale, Germany.
authors: Justin
authors: J
authors: Lee
authors: Department of Stress and Developmental Biology, Leibniz-Institute of Plant Biochemistry, Weinberg 3, 06120, Halle/Saale, Germany. dierk.scheel@ipb-halle.de.
authors: Dierk
authors: D
authors: Scheel
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D010748:Phosphopeptides / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D010748:Phosphopeptides; D010750:Phosphoproteins
keywords: Arabidopsis thaliana; Defense response; Enrichment; MAPK; MOAC; PAPE; Phosphopeptides; Phosphoproteins