title: Screening Stress Tolerance Traits in Arabidopsis Cell Cultures.
abstract: Screening for tolerance traits in plant cell cultures can combine the efficiency of microbial selection and plant genetics. Agrobacterium-mediated transformation can efficiently introduce cDNA library to cell suspension cultures generating population of randomly transformed microcolonies. Transformed cultures can subsequently be screened for tolerance to different stress conditions such as salinity, high osmotic, or oxidative stress conditions. cDNA inserts in tolerant cell lines can be easily identified by PCR amplification and homology search of the determined nucleotide sequences. The described methods have been tested and used to identify regulatory genes controlling salt tolerance in Arabidopsis. As cDNA libraries can be prepared from any plants, natural diversity can be explored by using extremophile plants as gene source.
authors: Institute of Plant Biology, Biological Research Centre, Temesvari krt. 62, 6726, Szeged, Hungary.
authors: Imma
authors: I
authors: Pérez-Salamó
authors: Institute of Plant Biology, Biological Research Centre, Temesvari krt. 62, 6726, Szeged, Hungary.
authors: Bogáta
authors: B
authors: Boros
authors: Institute of Plant Biology, Biological Research Centre, Temesvari krt. 62, 6726, Szeged, Hungary. szabados.laszlo@brc.mta.hu.
authors: László
authors: L
authors: Szabados
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics / Q000502:physiology; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D012965:Sodium Chloride
keywords: Agrobacterium; Gene isolation; In vitro selection; Transformation; cDNA library