title: The first determination of DNA sequence of a specific gene.
abstract: How and when the first DNA sequence of a gene was determined? In 1977, F. Sanger came up with an innovative technology to sequence DNA by using chain terminators, and determined the entire DNA sequence of the 5375-base genome of bacteriophage φX 174 (Sanger et al., 1977). While this Sanger's achievement has been recognized as the first DNA sequencing of genes, we had determined DNA sequence of a gene, albeit a partial sequence, 11 years before the Sanger's DNA sequence (Okada et al., 1966).
authors: Department of Biochemistry, Robert Wood Johnson Medical School, Rutgers University, United States. Electronic address: Inouye@cabm.rutgers.edu.
authors: Masayori
authors: M
authors: Inouye
mesh_terms: D004247:DNA / Q000266:history*; D049673:History, 20th Century; D006801:Humans; D017422:Sequence Analysis, DNA / Q000266:history* / Q000379:methods
chemical_list: D004247:DNA
keywords: First DNA sequencing; Frame-shift mutations; Sanger; T4 phage