title: Gal/GalNAc specific multiple lectins in marine bivalve Anadara granosa.
abstract: Complete lectin mapping of molluscs with their diversified recognition pattern and possible role in lectin-carbohydrate interaction based immune response triggering need much attention. In this communication, Gal/GalNAc specific three lectins AGL-IA (Anadara granosa lectin-IA), AGL-IB (A. granosa lectin-IB) and AGL-IV (A. granosa lectin-IV) and a lectin having hemolytic activity AGL-III (A. granosa lectin-III) were purified from the plasma of A. granosa bivalve by a combination of gel filtration and affinity chromatography. AGL-IA and IB were oligomeric lectins whereas, AGL-III and IV were monomeric. The molecular weight of AGL-IA, IB, III and IV were 375, 260, 45 and 33 kDa respectively. AGL-IA and IV agglutinated both rabbit and pronase treated human erythrocytes, whereas AGL-IB agglutinated only rabbit erythrocytes. AGL-III was found to agglutinate rabbit erythrocytes, however, it caused hemolysis of pronase treated human erythrocytes. The activity of all four lectins was calcium dependent and maximum at a pH range 7-8. Apart from Gal/GalNAc specific, the four lectins showed substantial differences in their carbohydrate recognition pattern. Moreover, there was a difference in the carbohydrate specificity between AGL-III and other three lectins (AGL-IA, AGL-IB and AGL-IV) towards polyvalent glycotope. On the one hand, 'cluster glycoside effect' i.e., an enhancement of the activity of a multivalent ligand, was observed for carbohydrate specificities of AGL-IA, AGL-IB, AGL-IV. On the other hand, the effect of multivalent ligands on the carbohydrate specificity of AGL-III was opposite of cluster glycoside effect. The affinity of AGL-IA, AGL-IB and AGL-IV for ligands can be ranked as follows: glycoproteins >> polysaccharide > oligosaccharides and monosaccharides. However, Gal related monosaccharides were the best inhibitors of AGL-III and the inhibitory activity decreased gradually in the following order: monosaccharide > disaccharide > polysaccharide. Thus, the diverse specificity of multiple lectins in A. granosa plasma possibly enables to recognize a wide range of microorganisms.
authors: Department of Chemistry, Faculty of Engineering and Technology, NSHM Knowledge Campus, Durgapur, West Bengal, India. Electronic address: mausumiadhya@gmail.com.
authors: Mausumi
authors: M
authors: Adhya
authors: Department of Chemistry, Bengal Institute of Technology and Management, Bolpur, West Bengal, India.
authors: Biswajit
authors: B
authors: Singha
mesh_terms: D000371:Agglutination; D000818:Animals; D049893:Arcidae / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D037102:Lectins
keywords: Anadara granosa; Carbohydrate-specificity; Hemolysin; Lectin; Marine molluscs