title: Multidrug Resistance Related to Biofilm Formation in Acinetobacter baumannii and Klebsiella pneumoniae Clinical Strains from Different Pulsotypes.
abstract: The emergence of Acinetobacter baumannii and Klebsiella pneumoniae strains in the hospital environment has been associated with the presence of multiple genetic elements, virulence factors and the ability to form biofilms. This study evaluated the biofilm formation ability of clinical and environmental A. baumannii and K. pneumoniae strains, isolated from various sources and presenting different molecular characteristics, resistance profiles and pulsed-field gel electrophoresis patterns. Fifty-three isolates were recovered from 2009 to 2014 in a Brazilian university hospital. Investigation of biofilm formation was performed for 10 strains of each species assessed by an initial adhesion assay, biofilm cell concentration and biofilm biomass, evaluated by quantitative assays in replicates, in three independent experiments. All strains of A. baumannii were able to attach to polystyrene plates, although two strains adhered to a lesser degree than the control. K. pneumoniae strains showed opposite behaviour, where only three strains adhered significantly when compared to the control. Quantitative evaluation revealed that in five A. baumannii and four K. pneumoniae isolates the biomass production could be characterised as moderate. None of the isolates were strong biofilm producers. Our results demonstrate: (1) biofilm formation is a heterogeneous property amongst A. baumannii and K. pneumoniae clinical strains and it was not associated with certain clonal types; (2) no relationship between multidrug resistance and biofilm production was observed; (3) more virulent K. pneumoniae strains tended to present higher production of biofilm.
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil.
authors: Paola Amaral
authors: PA
authors: de Campos
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil. sabriroyer@yahoo.com.br.
authors: Sabrina
authors: S
authors: Royer
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil.
authors: Deivid William da Fonseca
authors: DW
authors: Batistão
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil.
authors: Bruna Fuga
authors: BF
authors: Araújo
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil.
authors: Lícia Ludendorff
authors: LL
authors: Queiroz
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil.
authors: Cristiane Silveira
authors: CS
authors: de Brito
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil.
authors: Paulo P
authors: PP
authors: Gontijo-Filho
authors: Laboratory of Molecular Microbiology, Biomedical Science Institute, Federal University of Uberlândia, Av. Pará, 1720, Umuarama, Uberlândia, MG, 38400-902, Brazil.
authors: Rosineide Marques
authors: RM
authors: Ribas
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D001938:Brazil; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents