title: A label-free electrochemiluminescence immunosensor based on EuPO4 nanowire for the ultrasensitive detection of Prostate specific antigen.
abstract: EuPO4 nanowire, which exhibited strong and stable cathodic electrochemiluminescence (ECL) activity, was used for the first time to fabricate an immunosensor for the detection of prostate specific antigen (PSA). EuPO4 has some inherent excellent properties such as long luminescence lifetime, narrow emission band, high quantum yield and low toxicity. Based on these properties, a novel label-free ECL immunosensor was developed using EuPO4 as a sensing matrix. Chitosan solution was used to disperse EuPO4 nanowires and the amino groups on chitosan enabled the covalent attachment of capture antibodies. After the modification of the electrode surface with EuPO4 nanowires, anti-PSA was then immobilized on them, forming a label-free immunosensing interface. The specific binding of PSA on the electrode inhibited the ECL reaction of EuPO4 nanowires with the coreactant due to the steric hindrance effect (Deng et al., 2013). Under the optimum conditions, a good linear relationship between ECL intensity and the logarithm of PSA concentration was obtained in the range of 0.0005-80 ng/mL with a detection limit of 177.33 fg/mL. The proposed ECL immunosensor showed good stability, acceptable selectivity and reproducibility.
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China.
authors: Hongmin
authors: H
authors: Ma
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China.
authors: Jing
authors: J
authors: Zhou
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China.
authors: Yan
authors: Y
authors: Li
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China.
authors: Tongqian
authors: T
authors: Han
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China.
authors: Yong
authors: Y
authors: Zhang
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China.
authors: Lihua
authors: L
authors: Hu
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China. Electronic address: dubin61@gmail.com.
authors: Bin
authors: B
authors: Du
authors: School of Resources and Environment, University of Jinan, Jinan 250022, P. R. China.
authors: Qin
authors: Q
authors: Wei
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005063:Europium / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D010710:Phosphates / Q000737:chemistry; D017430:Prostate-Specific Antigen / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D010710:Phosphates; D005063:Europium; D048271:Chitosan; D017430:Prostate-Specific Antigen
keywords: Electrochemiluminescence immunosensor; EuPO(4) nanowire; Prostate specific antigen