title: Antibacterial efficacy of rifampin loaded solid lipid nanoparticles against Staphylococcus epidermidis biofilm.
abstract: OBJECTIVE
  The aim of the present study was to assess the in vitro anti-biofilm activities of solid lipid nanoparticles (SLN) loaded with rifampin against biofilm-producing Staphylococcus epidermidis.
  
  
  METHODS
  SLN were prepared and characterized for size, zeta potentials and encapsulation efficacy. The morphological and thermal properties of formulation were evaluated by TEM imagining and DSC analysis. The anti-biofilm activity of different formulations was assessed at different incubation times and concentrations by crystal violet (CV) and viable biofilm count methods.
  
  
  RESULTS
  The zeta potentials, particle sizes and encapsulation efficiencies of final formulations were 17 ± 0.7 mV, 101 ± 4.7 nm and approximately 70%; respectively. Rifampin-SLN was able to reduce the biomass of biofilm at time- and concentration-dependent manner. According to biofilm count results, the Rifampin-SLN was more effective for removal of the bacteria with respect to free rifampin.
  
  
  CONCLUSIONS
  The results of this study highlight the advantages and efficiency of Rifampin-SLN in biofilm eradication.
authors: Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Bibi Sedigheh
authors: BS
authors: Fazly Bazzaz
authors: Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Bahman
authors: B
authors: Khameneh
authors: Students Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Hamed
authors: H
authors: Zarei
authors: Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Golmohamadzadehsh@mums.ac.ir.
authors: Shiva
authors: S
authors: Golmohammadzadeh
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D004337:Drug Carriers / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012293:Rifampin / Q000737:chemistry* / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008055:Lipids; D012293:Rifampin
keywords: Bacterial biofilm; Bacterial count; Crystal violet; Rifampin; Solid lipid nanoparticles; Staphylococcus epidermidis