title: Preliminary Results of the Influence of Duodenojejunal Bypass in a Porcine Model of Streptozotocin-Induced Diabetes Mellitus.
abstract: BACKGROUND
  Type 2 diabetic obese patients present with a normalization of plasma glucose levels shortly after most bariatric procedures, before any significant weight loss takes place. There is only scarce literature in the new field of metabolic surgery, with most experiments being performed on small animal models.
  
  
  OBJECTIVE
  Our aim is to develop a reliable large animal model for assessment of surgical correction of diabetes.
  
  
  METHODS
  Titrated doses of streptozotocin (STZ) were used for induction of diabetes mellitus. After standardization of the surgical technique to avoid any restrictive component, three groups were created, a duodenojejunal bypass (DJB; n = 4), a gastroileal conduit (GIC; n = 3) near the ileocecal valve, and a sham (control; n = 5) group. Preoperative and postoperative glycemic curves were recorded by means of intravenous glucose tolerance tests. Body weight fluctuations were recorded as well.
  
  
  RESULTS
  Diabetes was successfully induced with the use of STZ in all cases. Animals in the sham group remained diabetic for 3 weeks after operation. There was normalization of blood glucose levels in the operative groups during the 3-week postoperative follow-up, without significant body weight changes. The duodenojejunal group resulted in stronger positive response of glycemia.
  
  
  CONCLUSIONS
  STZ-induced diabetes in swine leads to a reliable large animal model for assessment of metabolic surgical procedures. STZ is an effective but highly toxic means for inducing stable diabetes in the sensitive porcine model. Duodenojejunal bypass, although less invasive, seems to exert better antidiabetic effects than gastroileal conduit.
authors: Athens Medical Center, Karneadou 18, 10675, Athens, Greece. savashiridis@gmail.com.
authors: S
authors: S
authors: Hiridis
authors: Athens Medical Center, Karneadou 18, 10675, Athens, Greece.
authors: K
authors: K
authors: Konstantinidis
authors: 1st Propedeutic Surgical Clinic, "Hippokrateion" Hospital, Athens, Greece.
authors: E
authors: E
authors: Menenakos
authors: 1st Surgical Clinic, "Laiko" Hospital, Athina, Greece.
authors: Th
authors: T
authors: Diamantis
authors: Experimental-Research Center of ELPEN Pharmaceuticals, Athens, Greece.
authors: A
authors: A
authors: Papalois
authors: 1st Propedeutic Surgical Clinic, "Hippokrateion" Hospital, Athens, Greece.
authors: G
authors: G
authors: Zografos
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D050110:Bariatric Surgery; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000601:surgery*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000601:surgery*; D013505:Digestive System Surgical Procedures; D004195:Disease Models, Animal; D004386:Duodenum / Q000601:surgery; D005260:Female; D005763:Gastroenterostomy; D007082:Ileum / Q000601:surgery; D007583:Jejunum / Q000601:surgery; D013270:Stomach / Q000601:surgery; D013552:Swine
chemical_list: D001786:Blood Glucose
keywords: Bariatric procedures; Bypass; Streptozotocin-induced diabetes