title: Effects of sucrose amendment on ammonia assimilation during sewage sludge composting.
abstract: The aim of this study was to evaluate the laboratory-scale composting of sewage sludge and pumice mixtures that were amended with sucrose. The variation in temperature, pH, NH4(+)-N, ammonia emission, bacterial community, ammonia assimilating bacteria (AAB) populations and enzymatic activity related to ammonia assimilation were detected. The addition of sucrose increased the AAB population by 2.5-3.5 times, reduced ammonia emission by 24.7-31.1% compared with the control treatment, and promoted the growth of Bacillus and Wautersiella. The activities of glutamate dehydrogenase (GDH), glutamate synthase (GS) and glutamine synthetase (GOGAT), were enhanced by the addition of sucrose. GDH made a substantial contribution to ammonia assimilation when the ammonia concentration was high (⩾1.5g/kg) in the thermophilic phase. The GS/GOGAT cycle played an important role at low ammonia concentrations (⩽1.1g/kg) in the cooling phase. These results suggested that adding sucrose to sludge compost could promote ammonia assimilation and reduce ammonia emission.
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin, China; Institute of Microbiology, Heilongjiang Academy of Sciences, 150010 Harbin, China; Institute of Advanced Technology, Heilongjiang Academy of Sciences, 150020 Harbin, China.
authors: Liqiang
authors: L
authors: Meng
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin, China. Electronic address: hitlwg@126.com.
authors: Weiguang
authors: W
authors: Li
authors: Institute of Microbiology, Heilongjiang Academy of Sciences, 150010 Harbin, China; Institute of Advanced Technology, Heilongjiang Academy of Sciences, 150020 Harbin, China.
authors: Shumei
authors: S
authors: Zhang
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin, China.
authors: Chuandong
authors: C
authors: Wu
authors: School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090 Harbin, China.
authors: Ke
authors: K
authors: Wang
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000378:metabolism*; D064751:Ammonium Compounds / Q000302:isolation & purification; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase; D006863:Hydrogen-Ion Concentration; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000737:chemistry*; D012987:Soil; D013395:Sucrose / Q000494:pharmacology*; D013696:Temperature; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012987:Soil; D013395:Sucrose; D000641:Ammonia; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase
keywords: Ammonia assimilation; Ammonia emission; Composting; Sewage sludge; Sucrose