title: The protective effect of lycopene on hypoxia/reoxygenation-induced endoplasmic reticulum stress in H9C2 cardiomyocytes.
abstract: Nowadays, drugs protecting ischemia/reperfusion (I/R) myocardium become more suitable for clinic. It has been confirmed lycopene has various protections, but lacking the observation of its effect on endoplasmic reticulum stress (ERS)-mediated apoptosis caused by hypoxia/reoxygenation (H/R). This study aims to clarify the protective effect of lycopene on ERS induced by H/R in H9C2 cardiomyocytes. Detect the survival rate, lactic dehydrogenase (LDH) activity, apoptosis ratio, glucose-regulated proteins 78 (GRP78), C/EBP homologous protein (CHOP), c-Jun-N-terminal protein Kinase (JNK) and Caspase-12 mRNA and protein expression and phosphorylation of JNK (p-JNK) protein expression. LDH activity, apoptosis ratio and GRP78 protein expression increase in the H/R group, reduced by lycopene. The survival rate reduces in the H/R and thapsigargin (TG) groups; lycopene and 4-phenyl butyric acid (4-PBA) can improve it caused by H/R, lycopene also can improve it caused by TG. The apoptosis ratio, the expression of GRP78, CHOP and Caspase-12 mRNA and protein and p-JNK protein increase in the H/R and TG groups, weaken in the lycopene+H/R, 4-PBA+H/R and lycopene+TG groups. There is no obvious change in the expression of JNK mRNA or protein. Hence, our results provide the evidence that 10 μM lycopene plays an obviously protective effect on H/R H9C2 cardiomyocytes, realized through reducing ERS and apoptosis. The possible mechanism may be related to CHOP, p-JNK and Caspase-12 pathways.
authors: Department of Cardiology, The First Affiliated Hospital Of China Medical University, China. Electronic address: weihai19822008@163.com.
authors: Yang
authors: Y
authors: Gao
authors: Clinical Department of China Medical University, China. Electronic address: 1140651987@qq.com.
authors: Pengyu
authors: P
authors: Jia
authors: Department of Cardiology, The First Affiliated Hospital Of China Medical University, China. Electronic address: 493791589@qq.com.
authors: WenQi
authors: W
authors: Shu
authors: Department of Cardiology, The First Affiliated Hospital Of China Medical University, China. Electronic address: dalinjia2001@sina.com.
authors: Dalin
authors: D
authors: Jia
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002338:Carotenoids / Q000494:pharmacology*; D053454:Caspase 12 / Q000235:genetics; D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics; D048031:JNK Mitogen-Activated Protein Kinases / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D000077276:Lycopene; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051742:Transcription Factor CHOP / Q000235:genetics; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D002338:Carotenoids; D053454:Caspase 12; D006360:Heat-Shock Proteins; D048031:JNK Mitogen-Activated Protein Kinases; D007770:L-Lactate Dehydrogenase; D000077276:Lycopene; D010100:Oxygen; D012333:RNA, Messenger; D051742:Transcription Factor CHOP; C497251:Ddit3 protein, rat; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP
keywords: 2, 4-dinitrobenzene hydrazine (PubChem CID: 3772977); 4-phenyl butyric acid (PubChem CID: 2733848); CHOP; Caspase-12; Endoplasmic reticulum stress; GRP78; Hypoxia/reoxygenation; Lycopene; Lycopene (PubChem CID: 446925); dimethyl sulfoxide (PubChem CID: 679); lactic dehydrogenase (PubChem CID: 135297138); methylthiazolyltetrazolium (PubChem CID: 64965); sodium hydroxide (PubChem CID: 14798); thapsigargin (PubChem CID: 446378)