title: The anti-inflammatory effects of baicalin through suppression of NLRP3 inflammasome pathway in LPS-challenged piglet mononuclear phagocytes.
abstract: In this study, the anti-inflammatory effects and mechanisms of baicalin on LPS-induced NLRP3 inflammatory pathway were investigated in piglet mononuclear phagocytes (control, LPS stimulation, LPS stimulation + 12.5 µg/ml baicalin, LPS stimulation + 25 µg/ml baicalin, LPS stimulation + 50 µg/ml baicalin and LPS stimulation + 100 µg/ml baicalin). The levels of reactive oxygen species (ROS), the secretion levels of IL-1β, IL-18 and TNF-α, mRNA expression levels of IL-1β, IL-18, TNF-α and NLRP3, as well as the protein levels of cleaved caspase-1 p20 were significantly increased after LPS-challengein vitro However, LPS stimulation did not influence apoptosis-associated speck-like protein and caspase-1 mRNA levels, which are also components of the NLRP3 inflammasome. Baicalin at 50 µg/ml and 100 µg/ml could inhibit the production of ROS, TNF-α, IL-1β and IL-18, and down-regulate mRNA expression of IL-1β, IL-18, TNF-α and NLRP3, as well as expression of cleaved caspase-1 p20. These results showed that the anti-inflammatory effects of baicalin occurred via the regulation of the release of ROS and mRNA expression of NLRP3. The anti-inflammatory activity of baicalin could be related to the suppression of NLRP3 inflammasome pathway under LPS stimulation.
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan 430023, PR China.
authors: Chun
authors: C
authors: Ye
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China.
authors: Sali
authors: S
authors: Li
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China.
authors: Wenxu
authors: W
authors: Yao
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China.
authors: Lei
authors: L
authors: Xu
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan 430023, PR China qiuyinsheng6405@aliyun.com.
authors: Yinsheng
authors: Y
authors: Qiu
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan 430023, PR China.
authors: Yu
authors: Y
authors: Liu
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan 430023, PR China.
authors: Zhongyuan
authors: Z
authors: Wu
authors: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, PR China Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan 430023, PR China.
authors: Yongqing
authors: Y
authors: Hou
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D020170:Caspase 1 / Q000378:metabolism; D002478:Cells, Cultured; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D058847:Inflammasomes / Q000187:drug effects* / Q000502:physiology; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology; D020382:Interleukin-18 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D012157:Mononuclear Phagocyte System / Q000187:drug effects*; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein / Q000235:genetics / Q000378:metabolism*; D010586:Phagocytes / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D058847:Inflammasomes; D020382:Interleukin-18; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C038044:baicalin; D020170:Caspase 1
keywords: Baicalin; NLRP3 inflammasome; anti-inflammation; piglet peripheral blood mononuclear cell; reactive oxygen species