title: Point of care testing of phospholipase A2 group IIA for serological diagnosis of rheumatoid arthritis.
abstract: Secretory phospholipase A2 group IIA (sPLA2-IIA) was examined as a point of care marker for determining disease activity in rheumatoid (RA) and psoriatic (PsA) arthritis. Serum concentration and activity of sPLA2-IIA were measured using in-house antibodies and a novel point of care lateral flow device assay in patients diagnosed with varying severities of RA (n = 30) and PsA (n = 25) and found to correlate strongly with C-reactive protein (CRP). Levels of all markers were elevated in patients with active RA over those with inactive RA as well as both active and inactive PsA, indicating that sPLA2-IIA can be used as an analogue to CRP for RA diagnosis at point of care.
authors: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. sonya.abraham@imperial.nhs.uk m.stevens@imperial.ac.uk and Department of Medicine and Clinical Research Facility, Faculty of Medicine, Imperial College London, London, W12 0NN, UK.
authors: Nathan J
authors: NJ
authors: Liu
authors: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. sonya.abraham@imperial.nhs.uk m.stevens@imperial.ac.uk.
authors: Robert
authors: R
authors: Chapman
authors: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. sonya.abraham@imperial.nhs.uk m.stevens@imperial.ac.uk.
authors: Yiyang
authors: Y
authors: Lin
authors: Department of Medicine and Clinical Research Facility, Faculty of Medicine, Imperial College London, London, W12 0NN, UK.
authors: Jonas
authors: J
authors: Mmesi
authors: Mologic Ltd., Bedford Technology Park, Thurleigh, Bedfordshire MK44 2YP, UK.
authors: Andrew
authors: A
authors: Bentham
authors: Mologic Ltd., Bedford Technology Park, Thurleigh, Bedfordshire MK44 2YP, UK.
authors: Matthew
authors: M
authors: Tyreman
authors: Department of Medicine and Clinical Research Facility, Faculty of Medicine, Imperial College London, London, W12 0NN, UK.
authors: Sonya
authors: S
authors: Abraham
authors: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. sonya.abraham@imperial.nhs.uk m.stevens@imperial.ac.uk.
authors: Molly M
authors: MM
authors: Stevens
mesh_terms: D000328:Adult; D000368:Aged; D015535:Arthritis, Psoriatic / Q000175:diagnosis; D001172:Arthritis, Rheumatoid / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054501:Group II Phospholipases A2 / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D000067716:Point-of-Care Testing
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D054501:Group II Phospholipases A2; C516287:PLA2G2A protein, human