title: [Physicochemical Property of iRGD Peptide Modified Doxorubicin Loaded Liposome and Its Effect on Cancer Cell Growth].
abstract: OBJECTIVE
  To determine the physicochemical properties of iRGD conjugated doxorubicin loaded liposome (iRGD-LP-DOX), and its effect on targeting and inhibiting growth of A549 cells.
  
  
  METHODS
  Liposomes were observed under a transmission electron microscope. Release of doxorubicin from iRGD-LP-DOX was detected by the dialysis bag method. The efficiency of cellular uptake and tumor spheroids penetration on A549 cells in vitro was determined. The anti-proliferation efficiency of iRGD-LP-DOX was evaluated by MTT assay using IC50 (50% inhibition concentration).
  
  
  RESULTS
  iRGD-LP-DOX was spherical in a uniform size. Free DOX was released by 100% in 5 h, while LP-DOX and iRGD-LP-DOX were released by about 40% in 48 h. A higher level of iRGD-LP-DOX uptaken by A549 was found compared with that of LP-DOX (P<0.01). Higher fluorescence intensity was detected with iRGD-LP-DOX than with LP-DOX in tumor spheroid. The MTT assay confirmed strong inhibitory effect of iRGD-LP-DOX (P<0.01).
  
  
  CONCLUSIONS
  iRGD can enhance uptake of liposomes by A549 cells and inhibit the proliferation of tumor cells.
authors: Zhi-qiang
authors: ZQ
authors: Zhang
authors: Chun-lei
authors: CL
authors: Zhong
authors: Xin
authors: X
authors: Zhao
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; C569656:N-end cysteine peptide tumor-homing peptide; D009842:Oligopeptides; D004317:Doxorubicin