title: Simple and Reproducible Two-Stage Agitation Speed Control Strategy for Enhanced Triterpene Production by Lingzhi or Reishi Medicinal Mushrooms, Ganoderma lucidum ACCC G0119 (Higher Basidiomycetes) Based on Submerged Liquid Fermentation.
abstract: Triterpenes are important anticancer agents produced by batch submerged liquid fermentation, with the medicinal mushroom Ganoderma lucidum ACCC G0119, which was investigated under various dissolved oxygen levels by varying agitation speeds. Three kinetic parameters were analyzed: specific mycelial growth rate (μsmg), specific glucose consumption rate (qsgc), and specific triterpene production rate (qstp). High concentration, yield, and productivity of triterpenes were achieved by developing a simple and reproducible two-stage agitation speed control strategy. At the first 40 h, agitation speed was controlled at 150 rpm to obtain the quickest peak qstp for triterpene production, subsequently agitation speed was controlled at 100 rpm to maintain high qstp for high triterpene accumulation. The maximum concentration of triterpenes reached 0.086 g/l with the yield of 6.072 g/kg and the productivity of 6.532 × 10-4 g/(l·h), which were 39.61%, 36.48%, and 49.22%, respectively, better than the best results controlled by fixed agitation speeds. Conceivably, such a triterpene fermentation production strategy would be useful for industrial large-scale production of triterpenes with G. lucidum.
authors: Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, and Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors: Jie
authors: J
authors: Feng
authors: Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, and Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors: Na
authors: N
authors: Feng
authors: Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, and Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors: Yan
authors: Y
authors: Yang
authors: Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, and Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors: Fang
authors: F
authors: Liu
authors: Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, and Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors: Jingsong
authors: J
authors: Zhang
authors: Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, China. National Engineering Research Center of Edible Fungi, and Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China.
authors: Wei
authors: W
authors: Jia
authors: Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
authors: Chi-Chung
authors: CC
authors: Lin
mesh_terms: D019149:Bioreactors; D005285:Fermentation; D007700:Kinetics; D020940:Reishi / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D014315:Triterpenes