title: Infrared-active quadruple contrast FePt nanoparticles for multiple scale molecular imaging.
abstract: A single nanomaterial with multiple imaging contrasts and functions is highly desired for multiscale theragnosis. Herein, we demonstrate single 1-1.9 μm infrared-active FePt alloy nanoparticles (FePt NPs) offering unprecedented four-contrast-in-one molecular imaging - computed tomography (CT), magnetic resonance imaging (MRI), photoacoustic (PA) imaging, and high-order multiphoton luminescence (HOMPL) microscopy. The PA response of FePt NPs outperforms that of infrared-active gold nanorods by 3- to 5.6-fold under identical excitation fluence and particle concentrations. HOMPL (680 nm) of an isolated FePt NP renders spatial full-width-at-half-maximum values of 432 nm and 300 nm beyond the optical diffraction limit for 1230-nm and 920-nm excitation, respectively. The in vivo targeting function was successfully visualized using HOMPL, PA imaging, CT, and MRI, thereby validating FePt as a single nanomaterial system covering up to four types (Optical/PA/CT/MRI) of molecular imaging contrast, ranging from the microscopic level to whole-body scale investigation.
authors: Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors: Shang-Wei
authors: SW
authors: Chou
authors: Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors: Chien-Liang
authors: CL
authors: Liu
authors: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan. Electronic address: tmliu@ntu.edu.tw.
authors: Tzu-Ming
authors: TM
authors: Liu
authors: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan; 3D Printing Medical Research Center, China Medical University Hospital, Taichung City, Taiwan.
authors: Yu-Fang
authors: YF
authors: Shen
authors: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
authors: Lun-Chang
authors: LC
authors: Kuo
authors: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
authors: Cheng-Ham
authors: CH
authors: Wu
authors: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
authors: Tsung-Yuan
authors: TY
authors: Hsieh
authors: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
authors: Pei-Chun
authors: PC
authors: Wu
authors: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
authors: Ming-Rung
authors: MR
authors: Tsai
authors: Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013 Taiwan.
authors: Che-Chang
authors: CC
authors: Yang
authors: Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013 Taiwan.
authors: Kai-Yao
authors: KY
authors: Chang
authors: Institute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013 Taiwan.
authors: Meng-Hua
authors: MH
authors: Lu
authors: Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors: Pai-Chi
authors: PC
authors: Li
authors: Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors: Shi-Ping
authors: SP
authors: Chen
authors: Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors: Yu-Hsin
authors: YH
authors: Wang
authors: Department of Medical Imaging, Taipei TzuChi Hospital, The Buddhist Tzuchi Medical Foundation, New Taipei City 23142, Taiwan.
authors: Chen-Wen
authors: CW
authors: Lu
authors: Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
authors: Yi-An
authors: YA
authors: Chen
authors: Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
authors: Chih-Chia
authors: CC
authors: Huang
authors: Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi County 62102, Taiwan.
authors: Churng-Ren Chris
authors: CR
authors: Wang
authors: Department of Medical Imaging, Taipei TzuChi Hospital, The Buddhist Tzuchi Medical Foundation, New Taipei City 23142, Taiwan. Electronic address: jongkai@tzuchi.com.tw.
authors: Jong-Kai
authors: JK
authors: Hsiao
authors: Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013 Taiwan; Institute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013 Taiwan. Electronic address: mlli@ee.nthu.edu.tw.
authors: Meng-Lin
authors: ML
authors: Li
authors: Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan. Electronic address: chop@ntu.edu.tw.
authors: Pi-Tai
authors: PT
authors: Chou
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D049449:Luminescence; D008279:Magnetic Resonance Imaging; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D057054:Molecular Imaging; D043942:Nanotubes / Q000737:chemistry; D061088:Photoacoustic Techniques; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D014057:Tomography, X-Ray Computed
chemical_list: D003287:Contrast Media; D010984:Platinum; D007501:Iron
keywords: FePt nanoparticle; Infrared-active; Molecular imaging; Multi-modality contrast; Multiphoton luminescence; Photoacoustic responses