title: Contribution of Blastocystishominis subtypes and associated inflammatory factors in development of irritable bowel syndrome.
abstract: Blastocystis hominis with worldwide distribution is a human intestinal protozoa found in all countries. There have been differences in the severity of the pathogenesis of various Blastocystis spp. and a concomitant variation in the plasma concentration of the cytokines in patients with irritable bowel syndrome. In the present study, we aimed to demonstrate the contribution of B. hominis subtypes in the development of irritable bowel syndrome. Stool samples were collected from patients with gastrointestinal disorders. All samples were evaluated through native-lugol method. Total DNA was extracted. A PCR protocol was developed to amplify a specific region of the SSU ribosomal DNA (rDNA) gene. Serum levels of IL-6 and TNF-α were determined by immunoassay methods. The ClustalW algorithm was applied to align and blast the nucleotide sequences of the amplified region of the SSU rDNA gene. To evaluate the phylogenetic and molecular evolutionary of the nucleotide sequences, we used the MEGA software. In this study, we found 26 haplotypes of B. hominis in the studied samples which were collectively belong to five subtypes (ST1, ST2 in patients without irritable bowel syndrome vs. ST3 and two unknown subtypes in patients with irritable bowel syndrome). Result of ELISA showed a high level of IL-6 and TNF-α in the serum of patients with irritable bowel syndrome. The genetic heterogeneity of B. hominis and the existence of different subtypes of the protozoan in patients with IBS may shed light to the fact that some subtypes of parasites may involve in the pathogenesis of IBS.
authors: Department of Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.|Department of Biology, Islamic Azad University of Arak, Arak, Iran.
authors: Marzieh
authors: M
authors: Azizian
authors: Department of Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors: Gholam
authors: G
authors: Basati
authors: Department of Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors: Ghobad
authors: G
authors: Abangah
authors: Department of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
authors: Mohammad Reza
authors: MR
authors: Mahmoudi
authors: Department of Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran. amirzaeii@yahoo.com.
authors: Asad
authors: A
authors: Mirzaei
mesh_terms: D000328:Adult; D016844:Blastocystis / Q000235:genetics*; D016776:Blastocystis Infections / Q000469:parasitology*; D004275:DNA, Ribosomal / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005786:Gene Expression Regulation; D006239:Haplotypes; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D043183:Irritable Bowel Syndrome / Q000378:metabolism / Q000469:parasitology*; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D004275:DNA, Ribosomal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
keywords: Blastocystis hominis; ELISA; Heterogeneity; IBS