title: Paenibacillus periandrae sp. nov., isolated from nodules of Periandra mediterranea.
abstract: A bacterial strain designated PM10T was isolated from root nodules of Periandra mediterranea in Brazil. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Paenibacillus with its closest relatives being Paenibacillus vulneris CCUG 53270T and Paenibacillus yunnanensis YN2T with 95.6 and 95.9% 16S rRNA gene sequence similarity, respectively. The isolate was a Gram-stain-variable, motile, sporulating rod that was catalase-negative and oxidase-positive. Caseinase was positive, amylase was weakly positive and gelatinase was negative. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the only menaquinone detected and anteiso-C15 : 0 was the major fatty acid. Major polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and two unidentified lipids. meso-Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 52.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain PM10T should be considered representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus periandrae sp. nov. is proposed. The type strain is PM10T (=LMG 28691T=CECT 8827T).
authors: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca,Spain.
authors: Esther
authors: E
authors: Menéndez
authors: Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.
authors: Martha-Helena
authors: MH
authors: Ramírez-Bahena
authors: School of Biology, Newcastle University, Newcastle upon Tyne, UK.
authors: Lorena
authors: L
authors: Carro
authors: Instituto de Ciencias Agrarias, ICA-CSIC, Madrid, Spain.
authors: Mercedes
authors: M
authors: Fernández-Pascual
authors: School of Biology, Newcastle University, Newcastle upon Tyne, UK.
authors: Hans
authors: H
authors: Peter Klenk
authors: Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.|Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca,Spain.
authors: Encarna
authors: E
authors: Velázquez
authors: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca,Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.
authors: Pedro F
authors: PF
authors: Mateos
authors: Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.
authors: Alvaro
authors: A
authors: Peix
authors: Departamento de Botânica, Universidade Federal de Minas Gerais, Pampulha, Belo Horizonte, Brazil.
authors: Maria
authors: M
authors: Rita Scotti
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7