title: Robust Nanoparticle-DNA Conjugates Based on Mussel-Inspired Polydopamine Coating for Cell Imaging and Tailored Self-Assembly.
abstract: We have demonstrated that mussel-inspired polydopamine can serve as an intermediate coating layer for covalently attaching oligonucleotides on nanostructures of diverse chemical nature, which are made possible by the universal adhesion and spontaneous reactivity of polydopamine. Our results have shown that polydopamine can strongly bond to representative nanoparticles (i.e., Au nanoparticles and magnetic polymer nanobeads) and form a thin layer of coating that allows for attachment of commercially available DNA with thiol or amine end functionality. The resulting DNA-nanoparticle conjugates not only show excellent chemical and thermal stability and high loading density of DNA, but the linked DNA also maintain their biological functions in directing cancer cell targeting and undergo DNA hybridization to form multifunctional magnetic core-plasmonic satellite assemblies. The generally applicable strategy opens new opportunities for easy adoption of DNA-nanoparticle conjugates for broad applications in biosensors and nanomedicine.
authors: School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457.
authors: Chenxu
authors: C
authors: Wang
authors: School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457.
authors: Jiajing
authors: J
authors: Zhou
authors: School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457.|Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University , 1 Cleantech Loop, Singapore 637141.
authors: Peng
authors: P
authors: Wang
authors: Union Hospital, Tongji Medical College, Huazhong University of Science & Technology , Wuhan 430022, P. R. China.
authors: Wenshan
authors: W
authors: He
authors: School of Chemical and Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457.
authors: Hongwei
authors: H
authors: Duan
mesh_terms: D004247:DNA / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007211:Indoles; D011108:Polymers; C568283:polydopamine; D004247:DNA