title: Tectonic 1 Is a Key Regulator of Cell Proliferation in Pancreatic Cancer.
abstract: Pancreatic cancer is notoriously becoming one of the most devastating human cancers leading to death. However, clinical challenges still remain in diagnosis and treatment of this ticklish cancer. In the present study, the authors identified a new gene, Tectonic 1 (TCTN1), as a key regulator of cell proliferation in pancreatic cancer. Lentivirus-mediated short hairpin RNA (shRNA) was employed to knock down endogenous TCTN1 expression in PANC-1 pancreatic cancer cells. Knockdown of TCTN1 expression potently inhibited cell viability and proliferation, as determined by MTT and colony formation assays. Western blotting analysis also showed that knockdown of TCTN1 suppressed the expression of cdc2, while it induced that of p21 and p27. Flow cytometry analysis showed that depletion of TCTN1 in PANC-1 cells led to cell cycle arrest in the G2/M phase as well as apoptosis. Besides, depletion of TCTN1 led to the increase of Bax and cleavage of PARP-1, but the decrease of bcl2 by western blotting. The data indicate that TCTN1 is indispensable for pancreatic cancer cell proliferation, which provides a novel alternative to targeted therapy of pancreatic cancer and deserves further investigation.
authors: 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .
authors: Shiyong
authors: S
authors: Zhao
authors: 2 Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University , Harbin, China .
authors: Xuedong
authors: X
authors: Chen
authors: 3 Harbin Medical University , Harbin, China .
authors: Ming
authors: M
authors: Wan
authors: 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .
authors: Xingming
authors: X
authors: Jiang
authors: 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .
authors: Chunlong
authors: C
authors: Li
authors: 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .
authors: Yunfu
authors: Y
authors: Cui
authors: 1 Department of General Surgery, the Second Affiliated Hospital of Harbin Medical University , Harbin, China .
authors: Pengcheng
authors: P
authors: Kang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D016086:Lentivirus / Q000235:genetics; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C561486:Tctn1 protein, human
keywords: cell cycle; pancreatic cancer; proliferation; shRNA; tectonic 1