title: Identification of beer spoilage microorganisms using the MALDI Biotyper platform.
abstract: Beer spoilage microorganisms present a major risk for the brewing industry and can lead to cost-intensive recall of contaminated products and damage to brand reputation. The applicability of molecular profiling using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) in combination with Biotyper software was investigated for the identification of beer spoilage microorganisms from routine brewery quality control samples. Reference mass spectrum profiles for three of the most common bacterial beer spoilage microorganisms (Lactobacillus lindneri, Lactobacillus brevis and Pediococcus damnosus), four commercially available brewing yeast strains (top- and bottom-fermenting) and Dekkera/Brettanomyces bruxellensis wild yeast were established, incorporated into the Biotyper reference library and validated by successful identification after inoculation into beer. Each bacterial species could be accurately identified and distinguished from one another and from over 5600 other microorganisms present in the Biotyper database. In addition, wild yeast contaminations were rapidly detected and distinguished from top- and bottom-fermenting brewing strains. The applicability and integration of mass spectrometry profiling using the Biotyper platform into existing brewery quality assurance practices within industry were assessed by analysing routine microbiology control samples from a local brewery, where contaminating microorganisms could be reliably identified. Brewery-isolated microorganisms not present in the Biotyper database were further analysed for identification using LC-MS/MS methods. This renders the Biotyper platform a promising candidate for biological quality control testing within the brewing industry as a more rapid, high-throughput and cost-effective technology that can be tailored for the detection of brewery-specific spoilage organisms from the local environment.
authors: Adelaide Proteomics Centre, The University of Adelaide, Adelaide, Australia.|Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia.
authors: Michelle Elizabeth
authors: ME
authors: Turvey
authors: Adelaide Proteomics Centre, The University of Adelaide, Adelaide, Australia.|Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia.
authors: Florian
authors: F
authors: Weiland
authors: Coopers Brewery Ltd, Adelaide, Australia.
authors: Jon
authors: J
authors: Meneses
authors: Coopers Brewery Ltd, Adelaide, Australia.
authors: Nick
authors: N
authors: Sterenberg
authors: Adelaide Proteomics Centre, The University of Adelaide, Adelaide, Australia. peter.hoffmann@adelaide.edu.au.|Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, Australia. peter.hoffmann@adelaide.edu.au.
authors: Peter
authors: P
authors: Hoffmann
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D001515:Beer / Q000382:microbiology*; D002853:Chromatography, Liquid; D005516:Food Microbiology / Q000379:methods*; D005658:Fungi / Q000737:chemistry* / Q000145:classification* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
keywords: Beer spoilage microorganisms; Biotyper; MALDI; Mass spectrometry; Quality control