title: Synergistic effect of calcium and zinc on glucose/xylose utilization and butanol tolerance of Clostridium acetobutylicum.
abstract: Biobutanol outperforms bioethanol as an advanced biofuel, but is not economically competitive in terms of its titer, yield and productivity associated with feedstocks and energy cost. In this work, the synergistic effect of calcium and zinc was investigated in the acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum using glucose, xylose and glucose/xylose mixtures as carbon source(s). Significant improvements associated with enhanced glucose/xylose utilization, cell growth, acids re-assimilation and butanol biosynthesis were achieved. Especially, the maximum butanol and ABE production of 16.1 and 25.9 g L(-1) were achieved from 69.3 g L(-1) glucose with butanol/ABE productivities of 0.40 and 0.65 g L(-1) h(-1) compared to those of 11.7 and 19.4 g/L with 0.18 and 0.30 g L(-1) h(-1) obtained in the control respectively without any supplement. More importantly, zinc was significantly involved in the butanol tolerance based on the improved xylose utilization under various butanol-shock conditions (2, 4, 6, 8 and 10 g L(-1) butanol). Under the same conditions, calcium and zinc co-supplementation led to the best xylose utilization and butanol production. These results suggested that calcium and zinc could play synergistic roles improving ABE fermentation by C. acetobutylicum.
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Youduo
authors: Y
authors: Wu
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Chuang
authors: C
authors: Xue
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China ljchen@dlut.edu.cn.
authors: Lijie
authors: L
authors: Chen
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors: Wenjie
authors: W
authors: Yuan
authors: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors: Fengwu
authors: F
authors: Bai
mesh_terms: D000096:Acetone / Q000378:metabolism; D056804:Biofuels; D000440:Butanols / Q000378:metabolism* / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D004357:Drug Synergism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D015032:Zinc / Q000494:pharmacology*
chemical_list: D056804:Biofuels; D000440:Butanols; D004187:Disaccharides; C061908:xylose-glucose; D000096:Acetone; D000431:Ethanol; D015032:Zinc; D002118:Calcium
keywords: Clostridium acetobutylicum; butanol tolerance; calcium; synergistic effect; xylose; zinc