title: Potential diagnostic value of serum/pleural fluid IL-31 levels for tuberculous pleural effusion.
abstract: The aim of this study was to explore the diagnostic value of IL-31 levels in the pleural fluid and plasma to differentially diagnose tuberculous and malignant pleural effusion. We enrolled 91 cases, including tuberculous pleural effusion (TPE, n = 50), malignant pleural effusion (MPE, n = 41), other cases including pneumonia with pleural fluid, pulmonary tuberculosis and healthy people as controls. Whole blood was stimulated with the M. tuberculosis-specific antigens and plasma was collected. The multiplex bead-based cytokine immunoassay was employed to measure the levels of various cytokines. IL-31 was found to be the most prominent cytokine (P < 0.0001), and with an optimal cut-off value of 67.5 pg/mL, the sensitivity and specificity for the diagnosis of TPE were 86% and 100%, respectively. Furthermore, the tuberculosis-specific IL-31 levels in the plasma of TPE patients were higher than that of MPE patients (P = 0.0002). At an optimal cut-off value of 23.9 pg/mL, the sensitivity and specificity for the diagnosis of TPE were 92.9% and 85.7%, respectively. Ultimately, the combination of pleural fluid with the plasma tuberculosis-specific IL-31 levels improved the sensitivity and specificity to 94.0% and 95.1%, respectively. Thus, we identified a novel biomarker for the diagnosis of TPE for clinical application.
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Yan
authors: Y
authors: Gao
authors: Department of Pulmonary Diseases, Wuxi Infectious Diseases Hospital, Wuxi 214005, China.
authors: Qinfang
authors: Q
authors: Ou
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Jing
authors: J
authors: Wu
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Bingyan
authors: B
authors: Zhang
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Lei
authors: L
authors: Shen
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Shaolong
authors: S
authors: Chen
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Xinhua
authors: X
authors: Weng
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.|Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
authors: Ying
authors: Y
authors: Zhang
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.|Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Shanghai Medical College, and Institutes of Biomedical Science, Fudan University, Shanghai 200032, China.
authors: Wenhong
authors: W
authors: Zhang
authors: Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Lingyun
authors: L
authors: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D002648:Child; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007378:Interleukins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity / Q000502:physiology; D010996:Pleural Effusion / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D014396:Tuberculosis, Pleural / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; C502187:IL31 protein, human; D007378:Interleukins