title: Focal Adhesion Kinase Signaling Mediated the Enhancement of Osteogenesis of Human Mesenchymal Stem Cells Induced by Extracorporeal Shockwave.
abstract: Extracorporeal shockwave (ESW) has been shown of great potential in promoting the osteogenesis of bone marrow mesenchymal stem cells (BMSCs), but it is unknown whether this osteogenic promotion effect can also be achieved in other MSCs (i.e., tendon-derived stem cells (TDSCs) and adipose-derived stem cells (ADSCs)). In the current study, we aimed not only to compare the osteogenic effects of BMSCs induced by ESW to those of TDSCs and ADSCs; but also to investigate the underlying mechanisms. We show here that ESW (0.16 mj/mm(2)) significantly promoted the osteogenic differentiation in all the tested types of MSCs, accompanied with the downregulation of miR-138, but the activation of FAK, ERK1/2, and RUNX2. The enhancement of osteogenesis in these MSCs was consistently abolished when the cells were pretreated with one of the following conditions: overexpression of miR-138, FAK knockdown using specific siRNA, and U0126, implying that all of these elements are indispensable for mediating the effect of ESW. Moreover, our study provides converging genetic and molecular evidence that the miR-138-FAK-ERK1/2-RUNX2 machinery can be generally activated in ESW-preconditioned MSCs, suggesting that ESW may be a promising therapeutic strategy for the enhancement of osteogenesis of MSCs, regardless of their origins.
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.
authors: Jun
authors: J
authors: Hu
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.
authors: Haojie
authors: H
authors: Liao
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.
authors: Zebin
authors: Z
authors: Ma
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.
authors: Hongjiang
authors: H
authors: Chen
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.
authors: Zhonglian
authors: Z
authors: Huang
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.
authors: Yuantao
authors: Y
authors: Zhang
authors: The Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangdong Province, China.
authors: Menglei
authors: M
authors: Yu
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.
authors: Youbin
authors: Y
authors: Chen
authors: Department of Orthopaedics, the First Affiliated Hospital, Shantou University Medical College, Guangdong Province, China.|Department of Orthopaedics and Traumatology, Prince of Wales Hospital, Faculty of Medicine, the Chinese University of Hong Kong, Hong Kong SAR, China.
authors: Jiankun
authors: J
authors: Xu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D002070:Butadienes / Q000494:pharmacology; D002454:Cell Differentiation / Q000528:radiation effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D019220:High-Energy Shock Waves / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D010012:Osteogenesis / Q000235:genetics / Q000528:radiation effects*; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013710:Tendons / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects
chemical_list: D015703:Antigens, CD; D002070:Butadienes; D050659:Core Binding Factor Alpha 1 Subunit; C546110:MIRN138 microRNA, human; D035683:MicroRNAs; D009570:Nitriles; D034741:RNA, Small Interfering; C493709:RUNX2 protein, human; C113580:U 0126; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3