title: Assembly of designed protein scaffolds into monolayers for nanoparticle patterning.
abstract: The controlled assembly of building blocks to achieve new nanostructured materials with defined properties at different length scales through rational design is the basis and future of bottom-up nanofabrication. This work describes the assembly of the idealized protein building block, the consensus tetratricopeptide repeat (CTPR), into monolayers by oriented immobilization of the blocks. The selectivity of thiol-gold interaction for an oriented immobilization has been verified by comparing a non-thiolated protein building block. The physical properties of the CTPR protein thin biomolecular films including topography, thickness, and viscoelasticity, are characterized. Finally, the ability of these scaffolds to act as templates for inorganic nanostructures has been demonstrated by the formation of well-packed gold nanoparticles (GNPs) monolayer patterned by the CTPR monolayer.
authors: IMDEA-Nanociencia and Centro Nacional de Biotecnología (CNB-CSIC)-IMDEA Nanociencia Associated Unit, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors: Sara H
authors: SH
authors: Mejias
authors: IMDEA-Nanociencia and Centro Nacional de Biotecnología (CNB-CSIC)-IMDEA Nanociencia Associated Unit, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors: Pierre
authors: P
authors: Couleaud
authors: IMDEA-Nanociencia and Centro Nacional de Biotecnología (CNB-CSIC)-IMDEA Nanociencia Associated Unit, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors: Santiago
authors: S
authors: Casado
authors: IMDEA-Nanociencia and Centro Nacional de Biotecnología (CNB-CSIC)-IMDEA Nanociencia Associated Unit, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
authors: Daniel
authors: D
authors: Granados
authors: Instituto de Cerámica y Vidrio (ICV-CSIC), Cantoblanco, 28049 Madrid, Spain; Instituto de Magnetismo Aplicado "Salvador Velayos", UCM-ADIF, 28230 Madrid, Spain.
authors: Miguel Angel
authors: MA
authors: Garcia
authors: IMDEA-Nanociencia and Centro Nacional de Biotecnología (CNB-CSIC)-IMDEA Nanociencia Associated Unit, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain; Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: josemaria.abad@uam.es.
authors: Jose M
authors: JM
authors: Abad
authors: IMDEA-Nanociencia and Centro Nacional de Biotecnología (CNB-CSIC)-IMDEA Nanociencia Associated Unit, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain; CIC BiomaGUNE, Parque Tecnológico de San Sebastián, Paseo Miramón 182, Donostia-San Sebastián 20009, Spain. Electronic address: aitziber.lopezcortajarena@imdea.org.
authors: Aitziber L
authors: AL
authors: Cortajarena
mesh_terms: D000327:Adsorption; D006046:Gold / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D011506:Proteins / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D055682:Viscoelastic Substances / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D011506:Proteins; D013438:Sulfhydryl Compounds; D055682:Viscoelastic Substances; D006046:Gold
keywords: Biomolecular templating; Building blocks; Gold nanoparticles; Monolayer; Nanoparticle 2D arrays; Nanopatterning; Protein design; Repeat protein; Self-assembly