title: The features of mucosa-associated microbiota in primary sclerosing cholangitis.
abstract: BACKGROUND
  Little is known about the role of the microbiome in primary sclerosing cholangitis.
  
  
  OBJECTIVE
  To explore the mucosa-associated microbiota in primary sclerosing cholangitis (PSC) patients across different locations in the gut, and to compare it with inflammatory bowel disease (IBD)-only patients and healthy controls.
  
  
  METHODS
  Biopsies from the terminal ileum, right colon, and left colon were collected from patients and healthy controls undergoing colonoscopy. Microbiota profiling using bacterial 16S rRNA sequencing was performed on all biopsies.
  
  
  RESULTS
  Forty-four patients were recruited: 20 with PSC (19 with PSC-IBD and one with PSC-only), 15 with IBD-only and nine healthy controls. The overall microbiome profile was similar throughout different locations in the gut. No differences in the global microbiome profile were found. However, we observed significant PSC-associated enrichment in Barnesiellaceae at the family level, and in Blautia and an unidentified Barnesiellaceae at the genus level. At the operational taxa unit level, most shifts in PSC were observed in Clostridiales and Bacteroidales orders, with approximately 86% of shifts occurring within the former order.
  
  
  CONCLUSIONS
  The overall microbiota profile was similar across multiple locations in the gut from the same individual regardless of disease status. In this study, the mucosa associated-microbiota of patients with primary sclerosing cholangitis was characterised by enrichment of Blautia and Barnesiellaceae and by major shifts in operational taxa units within Clostridiales order.
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: J
authors: J
authors: Torres
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.|Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: X
authors: X
authors: Bao
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: A
authors: A
authors: Goel
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: J-F
authors: JF
authors: Colombel
authors: Section of Gastroenterology, Hepatology, and Nutrition, University of Chicago, Chicago, IL, USA.
authors: J
authors: J
authors: Pekow
authors: Section of Gastroenterology, Hepatology, and Nutrition, University of Chicago, Chicago, IL, USA.
authors: B
authors: B
authors: Jabri
authors: Section of Gastroenterology, Hepatology, and Nutrition, University of Chicago, Chicago, IL, USA.
authors: K M
authors: KM
authors: Williams
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: A
authors: A
authors: Castillo
authors: Division of Liver Diseases, Department of Medicine, Recanati/Miller Transplantation Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: J A
authors: JA
authors: Odin
authors: Section of Gastroenterology, Hepatology, and Nutrition, University of Chicago, Chicago, IL, USA.
authors: K
authors: K
authors: Meckel
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: F
authors: F
authors: Fasihuddin
authors: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: I
authors: I
authors: Peter
authors: Division of Gastroenterology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: S
authors: S
authors: Itzkowitz
authors: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
authors: J
authors: J
authors: Hu
mesh_terms: D000328:Adult; D000368:Aged; D015209:Cholangitis, Sclerosing / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology*; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology; D003113:Colonoscopy / Q000379:methods; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000473:pathology*; D008297:Male; D064307:Microbiota / Q000235:genetics; D008875:Middle Aged; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S