title: Involvement of the nervous system following experimental infection with Pasteurella multocida B:2 in buffalo (Bubalus bubalis): A clinicopathological study.
abstract: Haemorrhagic septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of Pasteurella multocida B:2 and E:2 in Asian and African, respectively. It is well known that HS affect mainly the respiratory and digestive tracts. However, involvement of the nervous system in pathogenesis of HS has been reported in previous studies without details. In this study, nine buffalo calves of 8 months old were distributed into three groups. Animals of Group 1 and 2 were inoculated orally and subcutaneously with 10 ml of 1 × 10(12) cfu/ml of P. multocida B:2, respectively, while animals of Group 3 were inoculated orally with 10 ml of phosphate buffer saline as a control. All calves in Group 1 and Group 3 were euthanised after 504 h (21 day) post-infection, while calves in Group 2 had to euthanise after 12 h post-infection as they develop sever clinical signs of HS. Significant differences were found in Group 2 in the mean scores of clinical signs, gross and histopathological changes which mainly affect different anatomic regions of the nervous system. In addition, successful bacterial isolation of P. multocida B:2 were obtained from different sites of the nervous system. On the other hand, less sever, clinical, gross and histopathological changes were found in Group 1. These results provide for the first time strong evidence of involving of the nervous system in pathogenesis of HS, especially in the peracute stage of the disease.
authors: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia; Department of Veterinary Internal Medicine, College of Veterinary Medicine, Al-Qasim Green University, Iraq.
authors: Ali Dhiaa
authors: AD
authors: Marza
authors: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia; Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia. Electronic address: jesseariasamy@gmail.com.
authors: Faez Firdaus Abdullah
authors: FF
authors: Jesse
authors: Department of Microbiology, College of Veterinary Medicine, University of Mosul, Iraq.
authors: Ihsan Muneer
authors: IM
authors: Ahmed
authors: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia.
authors: Eric Lim Teik
authors: EL
authors: Chung
authors: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia; Babil Technical Institute, Al Furat Al-Awsat Technical University, Iraq.
authors: Hayder Hamzah
authors: HH
authors: Ibrahim
authors: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia.
authors: Mohd
authors: M
authors: Zamri-Saad
authors: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Malaysia.
authors: Abdul Rahman
authors: AR
authors: Omar
authors: Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Malaysia.
authors: Md Zuki
authors: MZ
authors: Abu Bakar
authors: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia.
authors: Abdul Aziz
authors: AA
authors: Saharee
authors: Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia.
authors: Abdul Wahid
authors: AW
authors: Haron
authors: Department of Veterinary Pathology, College of Veterinary Medicine, University of Baghdad, Iraq.
authors: Mohammed Jwaid
authors: MJ
authors: Alwan
authors: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Malaysia.
authors: Mohd Azmi Mohd
authors: MA
authors: Lila
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle; D005260:Female; D006483:Hemorrhagic Septicemia / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D008297:Male; D009420:Nervous System / Q000469:parasitology* / Q000473:pathology; D016979:Pasteurella multocida / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
keywords: Buffaloes; Clinicopathological; Haemorrhagic septicaemia; Nervous system; Pasteurella multocida B:2