title: First airborne pathogen direct analysis system.
abstract: We report a portable "sample to answer" system for the rapid detection of airborne pathogens for the first time. The system contains a key microfluidic chip which fulfills both pathogen enrichment and biological identification functions. The system realizes simple operation and less human intervention as well as minimum reagent contamination. The operation is user-friendly and suitable for field and point-of-care applications. The system is capable of handling detection of different pathogens by changing the primers.
authors: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, 220 Handan Road, Shanghai, 200433, China. gsui@fudan.edu.cn.
authors: Qi
authors: Q
authors: Liu
authors: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, 220 Handan Road, Shanghai, 200433, China. gsui@fudan.edu.cn.
authors: Yuxiao
authors: Y
authors: Zhang
authors: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, 220 Handan Road, Shanghai, 200433, China. gsui@fudan.edu.cn and Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, 220 Handan Road, Shanghai, 200032, China.
authors: Wenwen
authors: W
authors: Jing
authors: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, 220 Handan Road, Shanghai, 200433, China. gsui@fudan.edu.cn.
authors: Sixiu
authors: S
authors: Liu
authors: Engineering Research Center of Optical Instrument and System, Ministry of Education and Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, 516 JunGong Road, Shanghai, 200093, China. usstoe@vip.163.com.
authors: Dawei
authors: D
authors: Zhang
authors: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science & Engineering, Fudan University, 220 Handan Road, Shanghai, 200433, China. gsui@fudan.edu.cn and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai, 200032, China.
authors: Guodong
authors: G
authors: Sui
mesh_terms: D000391:Air Microbiology; D056656:Lab-On-A-Chip Devices; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification; D013997:Time Factors