title: Insertion and deletion polymorphism in the alpha-2B adrenoceptor gene in pregnant women ripens gestational diabetes mellitus.
abstract: There are no earlier studies that reported the association of the 12Glu9 polymorphism in the alpha-2B adrenoceptor (ADRA2B) gene with gestational diabetes mellitus (GDM). We examined the potential association between the ADRA2B gene insertion/deletion (I/D) polymorphism in the Saudi population with GDM. Pregnant women with GDM have been reported to exhibit the same susceptibility as that observed in type 2 diabetes mellitus (T2DM). We have selected I/D polymorphism of the ADRA2B gene located in chromosome 2q11.1 that has been extensively related to T2DM and cardiovascular diseases. This case-control study was conducted with 200 GDM and 300 non-GDM pregnant women. Genotyping of I/D polymorphism was performed by conventional PCR method. Biochemical analyses were found to be significantly different between GDM and non-GDM subjects (p < 0.05). Genotype (ID + DD vs II, p = 0.0002) and allele (D vs I, p = 0.0002) frequencies of the 12Glu9 polymorphism were found to be statistically significant. However, a significant difference was found between allele and genotypes of I/D polymorphism of the ADRA2B gene or the clinical characteristics of the subjects. Our results obtained in this study indicate the ADRA2B gene in the Saudi women was associated with the development of GDM.
authors: Department of Obstetrics and Gynecology, King Khalid University Hospital, College of Medicine, King Saud University, P.O. Box 60826, Riyadh 11555, Saudi Arabia.
authors: Malak Mohammed
authors: MM
authors: Al-Hakeem
authors: Department of Obstetrics and Gynecology, King Khalid University Hospital, College of Medicine, King Saud University, P.O. Box 60826, Riyadh 11555, Saudi Arabia.
authors: Zeinab
authors: Z
authors: Abotalib
authors: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
authors: Khalid K
authors: KK
authors: Alharbi
authors: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
authors: Imran Ali
authors: IA
authors: Khan
authors: Centre of excellence in Biotechnology Research, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors: Arif A
authors: AA
authors: Mohammed
keywords: 12Glu9; ADRA2B gene; GDM; Insertion/deletion polymorphism; Saudi women; T2DM