title: Comparison of E,E-Farnesol Secretion and the Clinical Characteristics of Candida albicans Bloodstream Isolates from Different Multilocus Sequence Typing Clades.
abstract: Using multilocus sequence typing (MLST), Candida albicans can be subdivided into 18 different clades. Farnesol, a quorum-sensing molecule secreted by C. albicans, is thought to play an important role in the development of C. albicans biofilms and is also a virulence factor. This study evaluated whether C. albicans bloodstream infection (BSI) strains belonging to different MLST clades secrete different levels of E,E-farnesol (FOH) and whether they have different clinical characteristics. In total, 149 C. albicans BSI isolates from ten Korean hospitals belonging to clades 18 (n = 28), 4 (n = 23), 1 (n = 22), 12 (n = 17), and other clades (n = 59) were assessed. For each isolate, the FOH level in 24-hour biofilms was determined in filtered (0.45 μm) culture supernatant using high-performance liquid chromatography. Marked differences in FOH secretion from biofilms (0.10-6.99 μM) were observed among the 149 BSI isolates. Clade 18 isolates secreted significantly more FOH than did non-clade 18 isolates (mean ± SEM; 2.66 ± 0.22 vs. 1.69 ± 0.10 μM; P < 0.001). Patients with isolates belonging to clade 18 had a lower mean severity of illness than other patients, as measured using the "acute physiology and chronic health evaluation" (APACHE) III score (14.4 ± 1.1 vs. 18.0 ± 0.7; P < 0.05). This study provides evidence that C. albicans BSI isolates belonging to the most prevalent MLST clade (clade 18) in Korea are characterized by increased levels of FOH secretion and less severe illness.
authors: Department of Internal Medicine, Chonnam National University Medical School, Gwangju, South Korea.
authors: Sook-In
authors: SI
authors: Jung
authors: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea.
authors: Jong Hee
authors: JH
authors: Shin
authors: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea.
authors: Soo Hyun
authors: SH
authors: Kim
authors: Biomedical Research Institute, Chonnam National University Hospital, Gwangju, Korea.
authors: Jin
authors: J
authors: Kim
authors: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea.
authors: Joo Hee
authors: JH
authors: Kim
authors: Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea.
authors: Min Ji
authors: MJ
authors: Choi
authors: Department of Medical Education, Chonnam National University Medical School, Gwangju, South Korea.
authors: Eun-Kyung
authors: EK
authors: Chung
authors: Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.
authors: Kyungwon
authors: K
authors: Lee
authors: Department of Laboratory Medicine, Chungnam National University College of Medicine, Daejeon, South Korea.
authors: Sun Hoe
authors: SH
authors: Koo
authors: Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, South Korea.
authors: Hyun Ha
authors: HH
authors: Chang
authors: Institut Pasteur, Unité Biologie et Pathogenicité Fongiques, F-75015 Paris, France.|INRA, USC2019, F-75015 Paris, France.|Laboratoire de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Necker-Enfants Malades, Université Paris Descartes, Faculté de Médicine, F-75015 Paris, France.
authors: Marie-Elisabeth
authors: ME
authors: Bougnoux
authors: Institut Pasteur, Unité Biologie et Pathogenicité Fongiques, F-75015 Paris, France.|INRA, USC2019, F-75015 Paris, France.
authors: Christophe
authors: C
authors: d'Enfert
mesh_terms: D000328:Adult; D000368:Aged; D018441:Biofilms / Q000254:growth & development; D002176:Candida albicans / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D058387:Candidemia / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology / Q000382:microbiology* / Q000401:mortality; D005204:Farnesol / Q000378:metabolism*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D058885:Multilocus Sequence Typing
chemical_list: D005204:Farnesol