title: A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines.
abstract: RNA extraction resulting in good yields and quality is a fundamental step for the analyses of transcriptomes through high-throughput sequencing technologies, microarray, and also northern blots, RT-PCR, and RTqPCR. Even though many specific protocols designed for plants with high content of secondary metabolites have been developed, these are often expensive, time consuming, and not suitable for a wide range of tissues. Here we present a modification of the method previously described using the commercially available Concert™ Plant RNA Reagent (Invitrogen) buffer for field-grown adult pine trees with high oleoresin content.
authors: Molecular Genetics Laboratory, University of Passo Fundo, Passo Fundo, RS, Brazil.
authors: Júlio César
authors: JC
authors: de Lima
authors: Plant Physiology Laboratory, Center for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, 91501-970, RS, Brazil.|Department of Botany, UFRGS, Porto Alegre, RS, Brazil.
authors: Thanise Nogueira
authors: TN
authors: Füller
authors: Plant Physiology Laboratory, Department of Botany, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, 91501-970, RS, Brazil.|Center for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors: Fernanda
authors: F
authors: de Costa
authors: Regional Integrated University of Alto Uruguai and Missões (URI-FW), Frederico Westphalen, RS, Brazil.
authors: Kelly C S
authors: KC
authors: Rodrigues-Corrêa
authors: Plant Physiology Laboratory, Center for Biotechnology, Federal University of Rio Grande do Sul (UFRGS), CP 15005, Porto Alegre, 91501-970, RS, Brazil. fettneto@cbiot.ufrgs.br.|Department of Botany, UFRGS, Porto Alegre, RS, Brazil. fettneto@cbiot.ufrgs.br.
authors: Arthur G
authors: AG
authors: Fett-Neto
mesh_terms: D002021:Buffers; D028223:Pinus / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000096:biosynthesis / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D002021:Buffers; D010936:Plant Extracts; D018749:RNA, Plant; C018620:oleoresins
keywords: Concert plant RNA reagent; Conifers; Oleoresin; Pines; RNA; Stem RNA extraction