title: A long-term high-fat, high-sucrose diet in Bama minipigs promotes lipid deposition and amyotrophy by up-regulating the myostatin pathway.
abstract: Skeletal muscle is as an important regulator of blood glucose and glycolipid metabolism and is closely related to motor ability. The underlying mechanisms by which dietary ectopic lipids in skeletal muscle prevents muscle growth remain elusive. We utilized miniature Bama swine as a model to mimic human obesity using prolonged dietary induction. After 23 months on a high-fat, high-sucrose diet, metabolic disorders were induced in the animals, which exhibited increased body weight, extensive lipid deposition in the skeletal muscle and amyotrophy. Microarray profiles demonstrated the up-regulation of genes related to fat deposition and muscle growth inhibition. We outline a clear potential pathway that in combination with increased 11β-hydroxysteroid dehydrogenase type 1, promotes expression of a major inhibitor, myostatin, by converting corticosterone to cortisol, which leads to the growth inhibition of skeletal muscle. This research provides new insights into the treatment of muscle diseases induced by obesity.
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun, 130012, PR China.
authors: Jinxue
authors: J
authors: Ruan
authors: College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
authors: Yuanyuan
authors: Y
authors: Zhang
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China; College of Animal Science, Yangtz University, Jinzhou, 434023, Hubei, PR China.
authors: Jing
authors: J
authors: Yuan
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
authors: Leilei
authors: L
authors: Xin
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
authors: Jihan
authors: J
authors: Xia
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China; Agricutural Genomes Institute at Shenzhen, CAAS, Shenzhen, 518120, PR China.
authors: Nan
authors: N
authors: Liu
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
authors: Yulian
authors: Y
authors: Mu
authors: College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
authors: Yaoxing
authors: Y
authors: Chen
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China. Electronic address: yangshulin@caas.cn.
authors: Shulin
authors: S
authors: Yang
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Beijing, 100193, PR China; Agricutural Genomes Institute at Shenzhen, CAAS, Shenzhen, 518120, PR China.
authors: Kui
authors: K
authors: Li
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D050356:Lipid Metabolism; D024821:Metabolic Syndrome / Q000139:chemically induced / Q000150:complications* / Q000235:genetics; D009133:Muscular Atrophy / Q000209:etiology* / Q000235:genetics; D055435:Myostatin / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D013395:Sucrose / Q000008:administration & dosage / Q000009:adverse effects*; D013552:Swine; D013556:Swine, Miniature; D015854:Up-Regulation
chemical_list: D055435:Myostatin; D013395:Sucrose
keywords: Muscle mass; Obesity; Skeletal muscle