title: Effects of Bariatric Surgery on Serum Bile Acid Composition and Conjugation in a Diabetic Rat Model.
abstract: Serum bile acids (BAs) are elevated following bariatric surgery and have emerged as a potential glucose-lowering beneficial factor. The change of BA components and its underlying mechanisms may be of great significance during bariatric surgery. The aim of this study is to investigate the effects of different bariatric procedures on serum BA composition and explore the potential mechanisms using a diabetic rat model.
  
  
  
  Duodenal-jejunal bypass (DJB), sleeve gastrectomy (SG), and sham operation were performed in diabetic rats induced by high-fat diet (HFD) and streptozotocin (STZ). Body weight, food intake, oral glucose tolerance test (OGTT), and insulin tolerance test (ITT) were measured at indicated time points. Serum BAs composition and the expression of cholesterol 7α hydroxylase (CYP7A1), bile acid: CoA synthase (BACS) and bile acid-CoA: amino acid N-acyltransferase (BAAT) at both transcriptional and protein levels in the liver were evaluated at 12 weeks postoperatively.
  
  
  
  Compared with sham group, DJB and SG both achieved rapid and sustained improvements in glucose tolerance and insulin sensitivity. They also resulted in increased serum BAs, especially the taurine-conjugated BAs by elevated conjugation. No obvious difference was detected between DJB and SG except that SG achieved decreased weight gain and food intake.
  
  
  
  The preferentially elevated serum taurine-conjugated BAs were similar after different bariatric surgeries, and the enhanced conjugation of BAs in the liver might account for the changed serum BAs profiles.
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Qunzheng
authors: Q
authors: Wu
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Xiang
authors: X
authors: Zhang
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Mingwei
authors: M
authors: Zhong
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Haifeng
authors: H
authors: Han
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Shaozhuang
authors: S
authors: Liu
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Teng
authors: T
authors: Liu
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Meng
authors: M
authors: Wei
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Wei
authors: W
authors: Guo
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Haibin
authors: H
authors: Xie
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
authors: Sanyuan
authors: S
authors: Hu
authors: Department of General Surgery, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China. guangyongzhang@hotmail.com.
authors: Guangyong
authors: 0000-0001-5308-0129
authors: G
authors: Zhang
mesh_terms: D000818:Animals; D050110:Bariatric Surgery; D001647:Bile Acids and Salts / Q000097:blood* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000175:diagnosis / Q000378:metabolism / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000175:diagnosis / Q000378:metabolism / Q000601:surgery*; D004195:Disease Models, Animal; D005951:Glucose Tolerance Test; D007333:Insulin Resistance; D008297:Male; D009767:Obesity, Morbid / Q000097:blood* / Q000378:metabolism / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001647:Bile Acids and Salts
keywords: Bariatric surgery; Bile acids; Duodenal-jejunal bypass; Sleeve gastrectomy; Type 2 diabetes mellitus