title: The activity of three glycosidases (β-Ν-acetyloglucosaminidase, α-mannosidase, and β-galactosidase) in the follicular fluid and in the maturation medium affects bovine oocyte maturation.
abstract: We studied the role of follicular fluid's (FF) glycosidase (α-mannosidase [α-ΜΑΝ], β-Ν-acetyloglucosaminidase [NAGASE], β-galactosidase [β-GAL]) activity during IVM of bovine oocytes. Oocytes were allocated into two groups according to the follicular size (small follicle [SF]: 2-5 mm, large follicle [LF]: >5-8 mm). In experiment 1, cumulus-oocyte complexes (COCs) quality was evaluated according to morphologic criteria (grades A, B-C, D); oocyte (n = 801) nuclear maturation was assessed after 24 hours of incubation. Bovine embryos were produced in vitro in groups (experiment 2, n = 1503 oocytes) or individually (experiment 3, n = 50 oocytes). More grade-A and -BC COCs were collected from SF and LF groups, respectively (P < 0.05). Maturation rate (experiment 1) and cleavage rate (experiments 2 and 3) were similar in SF and LF groups. Activity of all glycosidases in FF was higher (P < 0.05) in SF group than in LF group, whereas in maturation medium of SF group it was, overall, significantly lower than in that of LF (experiments 2 and 3). In FF of SF group, NAGASE positively associated with grade-A oocytes and negatively with BC oocytes; increased β-GAL was associated with degenerated oocytes. Cleavage rate in LF group, related negatively to NAGASE and positively to α-MAN in maturation medium. These results indicate that during maturation, COCs release NAGASE and consume β-GAL, but differences probably exist between individual and group maturation.
authors: Department of Obstetrics & Reproduction, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
authors: E
authors: E
authors: Dovolou
authors: Hellenic Agricultural Organization - "DEMETER" (former NAGREF), Veterinary Research Institute of Thessaloniki, Thessaloniki, Greece.
authors: F
authors: F
authors: Samartzi
authors: Institut National de Recherche Agronomique (INRA), UMR7247, Physiologie de la Reproduction et des Comportements, Nouzilly, France.
authors: C
authors: C
authors: Perreau
authors: Department of Obstetrics & Reproduction, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
authors: F
authors: F
authors: Krania
authors: Institut National de Recherche Agronomique (INRA), UMR7247, Physiologie de la Reproduction et des Comportements, Nouzilly, France.
authors: A
authors: A
authors: Cordova
authors: Hellenic Agricultural Organization - "DEMETER" (former NAGREF), Veterinary Research Institute of Thessaloniki, Thessaloniki, Greece.
authors: E
authors: E
authors: Vainas
authors: Department of Obstetrics & Reproduction, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
authors: G S
authors: GS
authors: Amiridis
authors: Institut National de Recherche Agronomique (INRA), UMR7247, Physiologie de la Reproduction et des Comportements, Nouzilly, France.
authors: P
authors: P
authors: Mermillod
authors: Hellenic Agricultural Organization - "DEMETER" (former NAGREF), Veterinary Research Institute of Thessaloniki, Thessaloniki, Greece. Electronic address: tsiligianni@vri.gr.
authors: Th
authors: T
authors: Tsiligianni
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000662:veterinary; D003470:Culture Media; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods / Q000662:veterinary*; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism; D043323:alpha-Mannosidase / Q000378:metabolism* / Q000502:physiology; D001616:beta-Galactosidase / Q000378:metabolism* / Q000502:physiology
chemical_list: D003470:Culture Media; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D000118:Acetylglucosaminidase
keywords: Cumulus–oocyte complex; Follicle; Glycosidase; Maturation