title: Isolation and characterization of wound-induced compounds from the leaves of Citrus hassaku.
abstract: Citrus plants are world widely cultivated as horticultural tree crops, and nowadays their pharmacological activities have been well studied. Since research of defense responses in citrus plants have been mainly focused on the post-harvested fruits because of their commercial importance, defense mechanisms during their developmental stages have not been well understood. In the present study, two wound-induced compounds were isolated from leaves of Citrus hassaku, and their structures were elucidated by high-resolution electron spray ionization mass spectra (HRESIMS) and nuclear magnetic resonance (NMR) analyses. One of these compounds was identified as a known flavanone, hesperetin. The other was characterized as a novel furofuran lignan, and was named 'biscitrusnin-A'. Their antimicrobial activities were also evaluated.
authors: Graduated School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa City, Wakayama 649-6493, Japan.
authors: Tomonori
authors: T
authors: Asai
authors: Graduated School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa City, Wakayama 649-6493, Japan; Experimental Farm, Kinki University, Yuasa, Yuasa-cho, Arida-gun, Wakayama 643-0004, Japan.
authors: Tetsuya
authors: T
authors: Matsukawa
authors: Faculty of Agriculture, Tottori University, Koyama-cho, Tottori City, Tottori 680-8553, Japan.
authors: Atsushi
authors: A
authors: Ishihara
authors: Graduated School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa City, Wakayama 649-6493, Japan. Electronic address: kajiyama@waka.kindai.ac.jp.
authors: Shin'ichiro
authors: S
authors: Kajiyama
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification*; D002957:Citrus / Q000737:chemistry*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification; D006569:Hesperidin / Q000737:chemistry / Q000302:isolation & purification; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D010935:Plant Diseases; D018515:Plant Leaves / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014947:Wounds and Injuries
chemical_list: D000900:Anti-Bacterial Agents; D005663:Furans; D017705:Lignans; D006569:Hesperidin; C013015:hesperetin
keywords: Antibacterial activity; Citrus hassaku; Furofuran lignan; Mechanical wounding; Structure elucidation