title: Repair of Avascular Meniscus Tears with Electrospun Collagen Scaffolds Seeded with Human Cells.
abstract: The self-healing capacity of an injured meniscus is limited to the vascularized regions and is especially challenging in the inner avascular regions. As such, we investigated the use of human meniscus cell-seeded electrospun (ES) collagen type I scaffolds to produce meniscal tissue and explored whether these cell-seeded scaffolds can be implanted to repair defects created in meniscal avascular tissue explants. Human meniscal cells (derived from vascular and avascular meniscal tissue) were seeded on ES scaffolds and cultured. Constructs were evaluated for cell viability, gene expression, and mechanical properties. To determine potential for repair of meniscal defects, human meniscus avascular cells were seeded and cultured on aligned ES collagen scaffolds for 4 weeks before implantation. Surgical defects resembling "longitudinal tears" were created in the avascular zone of bovine meniscus and implanted with cell-seeded collagen scaffolds and cultured for 3 weeks. Tissue regeneration and integration were evaluated by histology, immunohistochemistry, mechanical testing, and magentic resonance imaging. Ex vivo implantation with cell-seeded collagen scaffolds resulted in neotissue that was significantly better integrated with the native tissue than acellular collagen scaffolds or untreated defects. Human meniscal cell-seeded ES collagen scaffolds may therefore be useful in facilitating meniscal repair of avascular meniscus tears.
authors: 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La Jolla, California.|2 Department of Material Science and Engineering, University of California , La Jolla, California.
authors: Jihye
authors: J
authors: Baek
authors: 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La Jolla, California.
authors: Sujata
authors: S
authors: Sovani
authors: 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La Jolla, California.
authors: Nicholas E
authors: NE
authors: Glembotski
authors: 3 Department of Radiology, School of Medicine, University of California , San Diego, San Diego, California.
authors: Jiang
authors: J
authors: Du
authors: 2 Department of Material Science and Engineering, University of California , La Jolla, California.
authors: Sungho
authors: S
authors: Jin
authors: 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La Jolla, California.
authors: Shawn P
authors: SP
authors: Grogan
authors: 1 Shiley Center for Orthopaedic Research and Education at Scripps Clinic , La Jolla, California.
authors: Darryl D
authors: DD
authors: D'Lima
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D055119:Elastic Modulus / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008279:Magnetic Resonance Imaging; D000072600:Meniscus / Q000473:pathology*; D010641:Phenotype; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D003094:Collagen