title: High-Resolution Melt Curve Analysis in Cancer Mutation Screen.
abstract: High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles.
authors: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Unit 72, 1515 Holcombe Blvd, Houston, TX, 77030, USA.
authors: Meenakshi
authors: M
authors: Mehrotra
authors: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Unit 72, 1515 Holcombe Blvd, Houston, TX, 77030, USA. kppatel@mdanderson.org.
authors: Keyur P
authors: KP
authors: Patel
mesh_terms: D005091:Exons; D006801:Humans; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D044366:Transition Temperature
chemical_list: D019009:Proto-Oncogene Proteins c-kit
keywords: Double-stranded DNA binding fluorescent dyes; High-resolution melt curve analysis; PCR