title: Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.
abstract: Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments.
authors: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, DD2 5DA, Scotland, UK. craig.simpson@hutton.ac.uk.
authors: Craig G
authors: CG
authors: Simpson
authors: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, DD2 5DA, Scotland, UK.
authors: John
authors: J
authors: Fuller
authors: Division of Plant Sciences, University of Dundee at The James Hutton Institute, Dundee, DD2 5DA, UK.
authors: Cristiane P G
authors: CP
authors: Calixto
authors: Biomathematics and Statistics Scotland, Invergowrie, DD2 5DA, Scotland, UK.
authors: Jim
authors: J
authors: McNicol
authors: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, DD2 5DA, Scotland, UK.
authors: Clare
authors: C
authors: Booth
authors: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, DD2 5DA, Scotland, UK.|Division of Plant Sciences, University of Dundee at The James Hutton Institute, Dundee, DD2 5DA, UK.
authors: John W S
authors: JW
authors: Brown
authors: Molecular Cell Physiology, Faculty of Biology, Bielefeld University, Universitaetsstrasse 25, Bielefeld, D-33615, Germany.
authors: Dorothee
authors: D
authors: Staiger
mesh_terms: D017398:Alternative Splicing / Q000235:genetics / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D057906:Circadian Clocks / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins
keywords: Alternative splicing; Circadian rhythm; RNA-binding proteins; Splicing factors