title: Effect and localization of phenanthrene in maize roots.
abstract: Polycyclic aromatic hydrocarbons (PAHs) have a toxic effect on plants, which limits the efficiency of phytomanagement of contaminated soils. The mechanisms underlying their toxicity are not fully understood. A cultivation experiment was carried out with maize, used as model plant, exposed to sand spiked with phenanthrene (50 or 150 mg kg(-1) dw). Epi-fluorescence microscopic observation of root sections was used to assess suberization of exodermis and endodermis and phenanthrene localization along the primary root length. For 10 days of cultivation, exodermis and endodermis suberization of exposed maize was more extensive. However, after 20 days of exposure, exodermis and endodermis of non-exposed roots were totally suberized, whilst PHE-exposed roots where less suberized. Early extensive suberization may act as barrier against PHE penetration, however longer exposure inhibits root maturation. Phenanthrene patches were located only near suberized exodermis and endodermis, which may therefore act as retention zones, where the hydrophobic phenanthrene accumulates during its radial transport.
authors: Université de Lorraine, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France; INRA, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France.
authors: Joan
authors: J
authors: Dupuy
authors: Université de Lorraine, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France; INRA, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France. Electronic address: pierre.leglize@univ-lorraine.fr.
authors: Pierre
authors: P
authors: Leglize
authors: Université de Lorraine, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France; INRA, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France.
authors: Quentin
authors: Q
authors: Vincent
authors: Université de Lorraine, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France; INRA, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France; Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, SK-845 38, Slovak Republic.
authors: Ivan
authors: I
authors: Zelko
authors: Université de Lorraine, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandœuvre-lès-Nancy, F-54506, France; CNRS, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Vandœuvre-lès-Nancy, F-54506, France.
authors: Christian
authors: C
authors: Mustin
authors: Université de Lorraine, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France; INRA, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France.
authors: Stéphanie
authors: S
authors: Ouvrard
authors: Université de Lorraine, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France; INRA, Laboratoire Sols et Environnement, UMR 1120, Vandœuvre-lès-Nancy, F-54518, France.
authors: Thibault
authors: T
authors: Sterckeman
mesh_terms: D004784:Environmental Monitoring; D008055:Lipids; D010616:Phenanthrenes / Q000378:metabolism / Q000633:toxicity*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism*; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
chemical_list: D008055:Lipids; D010616:Phenanthrenes; C031181:phenanthrene; C065875:suberin
keywords: Endodermis; Exodermis; Phenanthrene; Suberin lamellae