title: Comparative Detection and Quantification of Arcobacter butzleri in Stools from Diarrheic and Nondiarrheic People in Southwestern Alberta, Canada.
abstract: Arcobacter butzleri has been linked to enteric disease in humans, but its pathogenicity and epidemiology remain poorly understood. The lack of suitable detection methods is a major limitation. Using comparative genome analysis, we developed PCR primers for direct detection and quantification ofA. butzleri DNA in microbiologically complex matrices. These primers, along with existing molecular and culture-based methods, were used to detectA. butzleri and enteric pathogens in stools of diarrheic and nondiarrheic people (n= 1,596) living in southwestern Alberta, Canada, from May to November 2008. In addition, quantitative PCR was used to compare A. butzleridensities in diarrheic and nondiarrheic stools.Arcobacter butzleriwas detected more often by PCR (59.6%) than by isolation methods (0.8%). Comparison by PCR-based detection found no difference in the prevalence ofA. butzleri between diarrheic (56.7%) and nondiarrheic (45.5%) individuals. Rates of detection in diarrheic stools peaked in June (71.1%) and October (68.7%), but there was no statistically significant correlation between the presence ofA. butzleri and patient age, sex, or place of habitation. Densities ofA. butzleriDNA in diarrheic stools (1.6 ± 0.59 log10 copies mg(-1)) were higher (P= 0.007) than in nondiarrheic stools (1.3 ± 0.63 log10copies mg(-1)). Of the 892 diarrheic samples that were positive for A. butzleri, 74.1% were not positive for other bacterial and/or viral pathogens. The current study supports previous work suggesting that A. butzleri pathogenicity is strain specific and/or dependent on other factors, such as the level of host resistance.
authors: Agriculture and Agri-Food Canada, Lethbridge, AB, Canada Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.
authors: Andrew L
authors: AL
authors: Webb
authors: Department of Laboratory Medicine, Chinook Regional Hospital, Lethbridge, AB, Canada.
authors: Valerie F
authors: VF
authors: Boras
authors: Public Health Agency of Canada, Lethbridge, AB, Canada.
authors: Peter
authors: P
authors: Kruczkiewicz
authors: Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.
authors: L Brent
authors: LB
authors: Selinger
authors: Public Health Agency of Canada, Lethbridge, AB, Canada douglas.inglis@agr.gc.ca eduardo.taboada@phac-aspc.gc.ca.
authors: Eduardo N
authors: EN
authors: Taboada
authors: Agriculture and Agri-Food Canada, Lethbridge, AB, Canada douglas.inglis@agr.gc.ca eduardo.taboada@phac-aspc.gc.ca.
authors: G Douglas
authors: GD
authors: Inglis
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000416:Alberta; D020640:Arcobacter / Q000302:isolation & purification*; D058491:Bacterial Load; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000209:etiology* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D055815:Young Adult