title: Enhanced nutrient removal from municipal wastewater assisted by mixotrophic microalgal cultivation using glycerol.
abstract: In a present study, nutrient removal from municipal wastewater by Chlorella vulgaris and Nannochloropsis oculata was investigated by using mixotrophic cultivation with glycerol (0 to 5 g/L). Performance parameters were assessed by estimating the removal of total nitrogen, total phosphorus, chemical oxygen demand (COD), biomass growth, chlorophyll content, lipid yield, and fatty acids. With the addition of 2 g/L glycerol, a maximum biomass productivity of 56 mg/L/day was achieved in the mixotrophic culture of C. vulgaris within 12 days. The mixotrophic culture showed a 30-fold increase in biomass productivity compared to the wastewater without any glycerol. However, the highest total nitrogen removal (80.62 %), total phosphate removal (60.72 %), and COD removal (96.3 %) was observed in the N. oculata culture supplemented with 3, 5, and 1 g/L glycerol, respectively. These results suggest that mixotrophic cultivation using glycerol offers great potential in the production of renewable biomass, waste water treatment, and consequent production of high-value microalgal oil. Graphical Abstract Simultaneous biomass production and nutrient removal using microalgae cultivated in wastewater supplemented with glycerol.
authors: Department of Energy and Environment Convergence, Catholic Kwandong University, Gangneung, 210701, South Korea.
authors: Prabuddha L
authors: PL
authors: Gupta
authors: Department of Energy and Environment Convergence, Catholic Kwandong University, Gangneung, 210701, South Korea.
authors: Hee Jeong
authors: HJ
authors: Choi
authors: Department of Energy and Environment Convergence, Catholic Kwandong University, Gangneung, 210701, South Korea. leesm@cku.ac.kr.
authors: Seung-Mok
authors: SM
authors: Lee
mesh_terms: D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D048408:Chlorella vulgaris / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D058086:Microalgae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D002734:Chlorophyll; D005990:Glycerol; D009584:Nitrogen; D010758:Phosphorus; D010938:Plant Oils; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Biomass; Lipids; Microalgae; Mixotrophy; Wastewater treatment