title: Loss of UBE3A from TH-expressing neurons suppresses GABA co-release and enhances VTA-NAc optical self-stimulation.
abstract: Motivated reward-seeking behaviours are governed by dopaminergic ventral tegmental area projections to the nucleus accumbens. In addition to dopamine, these mesoaccumbal terminals co-release other neurotransmitters including glutamate and GABA, whose roles in regulating motivated behaviours are currently being investigated. Here we demonstrate that loss of the E3-ubiquitin ligase, UBE3A, from tyrosine hydroxylase-expressing neurons impairs mesoaccumbal, non-canonical GABA co-release and enhances reward-seeking behaviour measured by optical self-stimulation.
authors: Curriculum in Neurobiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Janet
authors: J
authors: Berrios
authors: Curriculum in Neurobiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Department of Psychiatry, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Inscopix Inc, Palo Alto, 94303 California, USA.
authors: Alice M
authors: AM
authors: Stamatakis
authors: Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Department of Psychiatry, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Pranish A
authors: PA
authors: Kantak
authors: Department of Psychiatry, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Zoe A
authors: ZA
authors: McElligott
authors: Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Matthew C
authors: MC
authors: Judson
authors: Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Megumi
authors: M
authors: Aita
authors: Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Marie
authors: M
authors: Rougie
authors: Curriculum in Neurobiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Department of Psychiatry, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Garret D
authors: GD
authors: Stuber
authors: Curriculum in Neurobiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Neuroscience Center, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.|Carolina Institute for Developmental Disabilities, University of North Carolina, Chapel Hill, 27599 North Carolina, USA.
authors: Benjamin D
authors: BD
authors: Philpot
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D004298:Dopamine / Q000378:metabolism*; D059290:Dopaminergic Neurons / Q000378:metabolism*; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009042:Motivation / Q000235:genetics*; D009434:Neural Pathways; D009474:Neurons / Q000378:metabolism; D009714:Nucleus Accumbens / Q000378:metabolism*; D062308:Optogenetics; D018408:Patch-Clamp Techniques; D012054:Reinforcement, Psychology; D012201:Reward; D012653:Self Stimulation; D013238:Stereotaxic Techniques; D009435:Synaptic Transmission / Q000235:genetics; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D017557:Ventral Tegmental Area / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid; D014446:Tyrosine 3-Monooxygenase; C502429:Ube3a protein, mouse; D044767:Ubiquitin-Protein Ligases; D004298:Dopamine