title: Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts.
abstract: A lipophilic oxygen radical absorbance capacity (L-ORAC) assay is an evaluation of the antioxidant capacity of solutions of lipophilic compounds. The concentrations of fluorescein, radical generator, and Trolox standard solutions were optimized to improve the precision of the assay. An interlaboratory study using two antioxidant solutions and three food extracts as test samples conducted in accordance with harmonized protocol demonstrated satisfactory L-ORAC measurements; the intermediate precision relative standard deviations (RSD(int)) ranged from 7.0 to 16.7%, the reproducibility relative standard deviations (RSD(R)) ranged from 14.8 to 19.4%, and the HorRat values ranged from 1.35 to 1.78.
authors: National Food Research Institute, National Agriculture and Food Research Organization.
authors: Jun
authors: J
authors: Watanabe
authors: Tomoyuki
authors: T
authors: Oki
authors: Jun
authors: J
authors: Takebayashi
authors: Hiroshi
authors: H
authors: Yada
authors: Manabu
authors: M
authors: Wagaki
authors: Yuko
authors: Y
authors: Takano-Ishikawa
authors: Akemi
authors: A
authors: Yasui
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002839:Chromans / Q000737:chemistry; D002935:Cinnamomum zeylanicum / Q000737:chemistry; D019793:Fluorescein / Q000737:chemistry; D005502:Food; D005504:Food Analysis / Q000379:methods*; D005609:Free Radicals / Q000737:chemistry; D020939:Zingiber officinale / Q000737:chemistry; D012275:Oryza / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D010666:Phenylpropionates / Q000737:chemistry; D011208:Powders; D017382:Reactive Oxygen Species / Q000737:chemistry*; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards; D024502:alpha-Tocopherol / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002839:Chromans; D005609:Free Radicals; D010666:Phenylpropionates; D011208:Powders; D017382:Reactive Oxygen Species; D012996:Solutions; D024502:alpha-Tocopherol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D010100:Oxygen; C013172:gamma-oryzanol; D019793:Fluorescein