title: Type I and II Diabetic Adipose-Derived Stem Cells RespondIn Vitroto Dehydrated Human Amnion/Chorion Membrane Allograft Treatment by Increasing Proliferation, Migration, and Altering Cytokine Secretion.
abstract: Objective:Human amniotic membranes have been shown to be effective for healing diabetic foot ulcers clinically and to regulate stem cell activityin vitroandin vivo; however, diabetic stem cells may be impaired as a sequela of the disease. In this study, dehydrated human amnion/chorion membrane (dHACM) allografts (EpiFix®; MiMedx Group) were evaluated for their ability to regulate diabetic stem cellsin vitro.Approach:Human adipose-derived stem cells (ADSCs) from normal, type I diabetic, and type II diabetic donors were treated with soluble extracts of dHACM and evaluated for proliferation after 3 days by DNA assay, chemotactic migration after 1 day by transwell assay, cytokine secretion after 3 days by multiplex ELISA, and gene expression after 5 days by reverse transcription-polymerase chain reaction.Results:Although diabetic ADSCs demonstrated decreased responses compared to normal ADSCs, dHACM treatment stimulated diabetic ADSCs to proliferate after 3 days and enhanced migration over 24 h, similar to normal ADSCs. dHACM-treated diabetic ADSCs modulated secretion of soluble signals, including regulators of inflammation, angiogenesis, and healing. All ADSCs evaluated also responded to dHACM treatment with altered expression of immunomodulatory genes, including interleukins (IL)-1α, IL-1β, and IL-1RA.Innovation:This is the first reported case demonstrating that diabetic ADSCs respond to novel amniotic membrane therapies, specifically treatment with dHACM.Conclusion:dHACM stimulated diabetic ADSCs to migrate, proliferate, and alter cytokine expression suggesting that, despite their diabetic origin, ADSCs may respond to dHACM to accelerate diabetic wound healing.
authors: MiMedx Group, Inc. , Marietta, Georgia .
authors: Michelle
authors: M
authors: Massee
authors: MiMedx Group, Inc. , Marietta, Georgia .
authors: Kathryn
authors: K
authors: Chinn
authors: MiMedx Group, Inc. , Marietta, Georgia .
authors: Jeremy J
authors: JJ
authors: Lim
authors: MiMedx Group, Inc. , Marietta, Georgia .
authors: Lisa
authors: L
authors: Godwin
authors: MiMedx Group, Inc. , Marietta, Georgia .
authors: Conan S
authors: CS
authors: Young
authors: MiMedx Group, Inc. , Marietta, Georgia .
authors: Thomas J
authors: TJ
authors: Koob