title: Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity.
abstract: Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.
authors: 1Institute for Multidisciplinary Research,University of Belgrade,Kneza Višeslava 1,11000 Belgrade,Serbia.
authors: Aleksandar
authors: A
authors: Savić
authors: 1Institute for Multidisciplinary Research,University of Belgrade,Kneza Višeslava 1,11000 Belgrade,Serbia.
authors: Aleksandra
authors: A
authors: Mitrović
authors: 3Scion,Private Bag 3020,Rotorua 3010,New Zealand.
authors: Lloyd
authors: L
authors: Donaldson
authors: 1Institute for Multidisciplinary Research,University of Belgrade,Kneza Višeslava 1,11000 Belgrade,Serbia.
authors: Jasna
authors: J
authors: Simonović Radosavljević
authors: 1Institute for Multidisciplinary Research,University of Belgrade,Kneza Višeslava 1,11000 Belgrade,Serbia.
authors: Jelena
authors: J
authors: Bogdanović Pristov
authors: 4Institute of Plant Biology,Biological Research Center,H-6701 Szeged,Hungary.
authors: Gabor
authors: G
authors: Steinbach
authors: 4Institute of Plant Biology,Biological Research Center,H-6701 Szeged,Hungary.
authors: Győző
authors: G
authors: Garab
authors: 1Institute for Multidisciplinary Research,University of Belgrade,Kneza Višeslava 1,11000 Belgrade,Serbia.
authors: Ksenija
authors: K
authors: Radotić
mesh_terms: D055592:Biophysical Phenomena; D002473:Cell Wall / Q000737:chemistry* / Q000648:ultrastructure*; D002482:Cellulose / Q000032:analysis*; D008856:Microscopy, Fluorescence; D028222:Picea / Q000166:cytology*; D059828:Plant Cells / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D002482:Cellulose
keywords: Picea omorika (Pančić) Purkynĕ; cell wall; cellulose fibrils; compression wood severity; fluorescence-detected linear dichroism microscopy