title: TOR (target of rapamycin) is a key regulator of triacylglycerol accumulation in microalgae.
abstract: Most microalgae abundantly accumulate lipid droplets (LDs) containing triacylglycerols (TAGs) under several stress conditions, but the underlying molecular mechanism of this accumulation remains unclear. In a recent study, we found that inhibition of TOR (target of rapamycin), a highly conserved protein kinase of eukaryotes, by rapamycin resulted in TAG accumulation in microalgae, indicating that TOR negatively regulates TAG accumulation. Here, we show that formation of intracellular LDs and TAG accumulation were also induced in the unicellular green alga Chlamydomonas reinhardtii after exposure to Torin1 or AZD8055, which are novel TOR inhibitors that inhibit TOR activity in a manner different from rapamycin. These results supported quite well our previous conclusion that TOR is a central regulator of TAG accumulation in microalgae.
authors: a Chemical Resources Laboratory, Tokyo Institute of Technology , Yokohama , Japan.|b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Saitama , Japan.
authors: Sousuke
authors: S
authors: Imamura
authors: a Chemical Resources Laboratory, Tokyo Institute of Technology , Yokohama , Japan.
authors: Yasuko
authors: Y
authors: Kawase
authors: a Chemical Resources Laboratory, Tokyo Institute of Technology , Yokohama , Japan.
authors: Ikki
authors: I
authors: Kobayashi
authors: c Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , Yokohama , Japan.
authors: Mie
authors: M
authors: Shimojima
authors: b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Saitama , Japan.|c Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology , Yokohama , Japan.|d Earth-Life Science Institute, Tokyo Institute of Technology , Tokyo , Japan.
authors: Hiroyuki
authors: H
authors: Ohta
authors: a Chemical Resources Laboratory, Tokyo Institute of Technology , Yokohama , Japan.|b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST) , Saitama , Japan.
authors: Kan
authors: K
authors: Tanaka
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D058086:Microalgae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D009025:Morpholines / Q000494:pharmacology; D009287:Naphthyridines / Q000494:pharmacology; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: C553294:1-(4-(4-propionylpiperazin-1-yl)-3-(trifluoromethyl)phenyl)-9-(quinolin-3-yl)benzo(h)(1,6)naphthyridin-2(1H)-one; D009025:Morpholines; D009287:Naphthyridines; D014280:Triglycerides; C546624:(5-(2,4-bis((3S)-3-methylmorpholin-4-yl)pyrido(2,3-d)pyrimidin-7-yl)-2-methoxyphenyl)methanol; D058570:TOR Serine-Threonine Kinases
keywords: AZD8055; Chlamydomonas reinhardtii; Cyanidioschyzon merolae; TOR inhibitor; Torin1; lipid droplet; microalga; target of rapamycin; triacylglycerol