title: Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids.
abstract: Human biofluids, especially blood plasma or serum, hold great potential as the sources of candidate biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge for detecting promising low-abundance proteins. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely applied for separating low-abundance proteins from the high- and moderate-abundance proteins, thus enabling much more effective detection of low-abundance proteins. Herein, we review the advances of immunoaffinity separation methods and their contributions to the proteomic applications in human biofluids. The limitations and future perspectives of immunoaffinity separation methods are also discussed.
authors: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States.
authors: Chaochao
authors: C
authors: Wu
authors: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States.
authors: Jicheng
authors: J
authors: Duan
authors: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States.
authors: Tao
authors: T
authors: Liu
authors: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States.
authors: Richard D
authors: RD
authors: Smith
authors: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, United States. Electronic address: weijun.qian@pnnl.gov.
authors: Wei-Jun
authors: WJ
authors: Qian
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002846:Chromatography, Affinity; D006801:Humans; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: Affinity proteomics; Biofluid; Biomarker discovery; Immunoaffinity chromatography; Immunodepletion; Immunoenrichment; Plasma/Serum; Proteomics