title: Human C5a Protein Participates in the Mosquito Immune Response Against Dengue Virus.
abstract: Dengue virus (DENV) is transmitted by Aedes spp mosquitoes during a bloodmeal uptake. The bloodmeal consists of host cells, immune factors, and possibly blood-borne pathogens, such as arboviruses. Human cells and immune-related factors, like the complement system, can remain active in the bloodmeal and may be able to interact with pathogens in the mosquito. Previous studies have shown that active complement proteins impact Plasmodium parasite viability in the Anopheles midgut. Thus, we investigated the effects of the human complement on DENV infection in the midgut of Aedes aegypti. Our findings indicate that mosquitoes receiving DENV mixed with normal non-inactivated human serum showed significantly lower viremia than those fed with heat-inactivated serum. This implies that human complement may act to limit DENV infection in the mosquito midgut. In addition, we found that human complement C5a protein was able to directly communicate with mosquito cells, affecting the cell antiviral response against DENV. Our results also show that human C5a protein is able to interact with several membrane-bound mosquito proteins. Together these results suggest an important role of human complement protein in DENV transmission.
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, 29209 (berlinlondo@yahoo.com; Andrea.Troupin@uscmed.sc.edu), berlinlondo@yahoo.com.
authors: Berlin
authors: B
authors: Londono-Renteria
authors: Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA, 70130 (cgrippin@tulane.edu), and.
authors: Crystal
authors: C
authors: Grippin
authors: Microbiology and Clinical Laboratory, Hospital San Juan de Dios, Los Patios - Norte de Santander, Colombia (carocardenasg@hotmail.com).
authors: Jenny C
authors: JC
authors: Cardenas
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, 29209 (berlinlondo@yahoo.com; Andrea.Troupin@uscmed.sc.edu).
authors: Andrea
authors: A
authors: Troupin
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, 29209 (berlinlondo@yahoo.com; Andrea.Troupin@uscmed.sc.edu).
authors: Tonya M
authors: TM
authors: Colpitts
mesh_terms: D000330:Aedes / Q000235:genetics / Q000276:immunology* / Q000821:virology; D000818:Animals; D015936:Complement C5a / Q000276:immunology*; D003715:Dengue / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007303:Insect Vectors / Q000235:genetics / Q000276:immunology* / Q000821:virology
chemical_list: D015936:Complement C5a
keywords: Aedes; dengue; human complement; mosquito