title: Heat-pretreatment and enzymolysis behavior of the lotus seed protein.
abstract: Lotus seed protein (LSP) was heat-pretreated before enzymolysis in order to seek a greater degree of hydrolysis (DH) during enzymatic hydrolysis. The parameters including substrate concentration, temperature, pH, and papain concentration were optimized by response surface methodology in the enzymolysis of the heat-pretreated LSP. The influence of substrate concentration on the non-pretreated LSP enzymolysis was assessed, and the enzymolysis was found to obey the Haldane model with inhibition by LSP substrate. The initial concentration of non-pretreated LSP was inferred theoretically to be 11.07 g/L in order to avoid substrate inhibition. On the other hand, Chrastil model was fitted and the diffusion resistance constant values were in the range of 0.5-0.6 for the diffusion-controlled encounter of enzyme and substrate, implying that diffusion was a rate-limiting step. The heat-pretreatment at 60 °C for 60 min could increase the DH of the LSP, which enhanced the efficiency of the enzymolysis by papain.
authors: Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors: A-Dan
authors: AD
authors: Pan
authors: Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China. Electronic address: hongyanzeng99@hotmail.com.
authors: Hong-Yan
authors: HY
authors: Zeng
authors: Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors: Gohi Bi Foua Claude
authors: GB
authors: Alain
authors: Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, PR China.
authors: Bo
authors: B
authors: Feng
mesh_terms: D006358:Hot Temperature; D000070116:Lotus; D011506:Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry / Q000201:enzymology*
chemical_list: D011506:Proteins
keywords: Disodium hydrogen phosphate (PubChem CID: 24203); Enzymolysis; Heat-pretreatment; Hydrochloric acid (PubChem CID: 313); Kinetic models; Lotus seed protein; Monosodium phosphate (PubChem CID: 23672064); Response surface methodology; Sodium chloride (PubChem CID: 5234); Trichloroacetic acid (PubChem CID: 6421)