title: Plant extract-mediated biogenic synthesis of silver, manganese dioxide, silver-doped manganese dioxide nanoparticles and their antibacterial activity against food- and water-borne pathogens.
abstract: Silver nanoparticles (AgNPs), manganese dioxide nanoparticles (MnO₂NPs) and silver-doped manganese dioxide nanoparticles (Ag-doped MnO₂NPs) were synthesized by simultaneous green chemistry reduction approach. Aqueous extract from the leaves of medicinally important plant Cucurbita pepo was used as reducing and capping agents. Various characterization techniques were carried out to affirm the formation of nanoparticles. HR-TEM analysis confirmed the size of nanoparticles in the range of 15-70 nm and also metal doping was confirmed through XRD and EDS analyses. FT-IR analysis confirmed that the presence of biomolecules in the aqueous leaves extract was responsible for nanoparticles synthesis. Further, the concentration of metals and their doping in the reaction mixture was achieved by ICP-MS. The growth curve and well diffusion study of synthesized nanoparticles were performed against food- and water-borne Gram-positive and Gram-negative bacterial pathogens. The mode of interaction of nanoparticles on bacterial cells was demonstrated through Bio-TEM analysis. Interestingly, AgNPs and Ag-doped MnO₂NPs showed better antibacterial activity against all the tested bacterial pathogens; however, MnO₂NPs alone did not show any antibacterial properties. Hence, AgNPs and Ag-doped MnO₂NPs synthesized from aqueous plant leaves extract may have important role in controlling various food spoilage caused by bacteria.
authors: Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors: Chandran
authors: C
authors: Krishnaraj
authors: Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors: Byoung-Jun
authors: BJ
authors: Ji
authors: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331, USA.
authors: Stacey L
authors: SL
authors: Harper
authors: Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, 561-756, Republic of Korea. siyun@jbnu.ac.kr.
authors: Soon-Il
authors: SI
authors: Yun
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005516:Food Microbiology; D017895:Manganese Compounds / Q000737:chemistry*; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D033362:Powder Diffraction; D012834:Silver / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D017895:Manganese Compounds; D010087:Oxides; D010936:Plant Extracts; D012834:Silver; C016552:manganese dioxide
keywords: Ag-doped MnO2NPs; AgNPs; Antibacterial activity; Cucurbita pepo leaves extract; MnO2NPs