title: The Effect of Lactobacillus crispatus and Lactobacillus rhamnosusCulture Supernatants on Expression of Autophagy Genes and HPV E6 and E7 Oncogenes in The HeLa Cell Line.
abstract: OBJECTIVE
  The aim of this study was to clarify the mechanism by which lactobacilli exert their cytotoxic effects on cervical cancer cells. In addition, we aimed to evalu- ate the effect of lactobacilli on the expression of human papilloma virus (HPV) onco- genes.
  
  
  METHODS
  In this experimental study, using quantitative real-time polymer- ase chain reaction (PCR), we analyzed the expression of CASP3 and three autophagy genes [ATG14, BECN1 and alpha 2 catalytic subunit of AMPK (PRKAA2)] along with HPV18 E6 and E7 genes in HeLa cells before and after treatment with Lactobacillus crispatus and Lactobacillus rhamnosus culture supernatants.
  
  
  RESULTS
  The expression of CASP3 and autophagy genes in HeLa cells was de- creased after treatment with lactobacilli culture supernatants. However, this de- crease was not significant for PRKAA2 when compared with controls. In addition, expression of HPV E6 was significantly decreased after treatment with lactobacilli culture supernatants.
  
  
  CONCLUSIONS
  Lactobacilli culture supernatants can decrease expression of ATG14 and BECN1 as well as the HPV E6 oncogene. It has been demonstrated that the main changes occurring during cervical carcinogenesis in cell machinery can be reversed by suppression of HPV oncogenes. Therefore, downregulation of HPV E6 by lacto- bacilli may have therapeutic potential for cervical cancer. As the role of autophagy in cancer is complicated, further work is required to clarify the link between downregula- tion of autophagy genes and antiproliferative effects exerted by lactobacilli.
authors: Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Elahe
authors: E
authors: Motevaseli
authors: Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors: Rosa
authors: R
authors: Azam
authors: Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors: Seyed Mohammad
authors: SM
authors: Akrami
authors: Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammadali
authors: M
authors: Mazlomy
authors: Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mojtaba
authors: M
authors: Saffari
authors: Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors: Mohammad Hossein
authors: MH
authors: Modarressi
authors: Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
authors: Maryam
authors: M
authors: Daneshvar
authors: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Soudeh
authors: S
authors: Ghafouri-Fard
keywords: Autophagy; HPV; Lactobacillus