title: Gold Nanoparticles Doped with (199) Au Atoms and Their Use for Targeted Cancer Imaging by SPECT.
abstract: Gold nanoparticles have been labeled with various radionuclides and extensively explored for single photon emission computed tomography (SPECT) in the context of cancer diagnosis. The stability of most radiolabels, however, still needs to be improved for accurate detection of cancer biomarkers and thereby monitoring of tumor progression and metastasis. Here, the first synthesis of Au nanoparticles doped with (199)Au atoms for targeted SPECT tumor imaging in a mouse triple negative breast cancer (TNBC) model is reported. By directly incorporating (199)Au atoms into the crystal lattice of each Au nanoparticle, the stability of the radiolabel can be ensured. The synthetic procedure also allows for a precise control over both the radiochemistry and particle size. When conjugated with D-Ala1-peptide T-amide, the Au nanoparticles doped with (199)Au atoms can serve as a C-C chemokine receptor 5 (CCR5)-targeted nanoprobe for the sensitive and specific detection of both TNBC and its metastasis in a mouse tumor model.
authors: Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors: Yongfeng
authors: Y
authors: Zhao
authors: The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30332, USA.|Department of Biomedical Engineering, Peking University, Beijing, 100871, P. R. China.
authors: Bo
authors: B
authors: Pang
authors: Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors: Hannah
authors: H
authors: Luehmann
authors: Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors: Lisa
authors: L
authors: Detering
authors: The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30332, USA.
authors: Xuan
authors: X
authors: Yang
authors: Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors: Deborah
authors: D
authors: Sultan
authors: Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors: Scott
authors: S
authors: Harpstrite
authors: Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors: Vijay
authors: V
authors: Sharma
authors: Research Reactor Center, University of Missouri, Columbia, MO, 65211, USA.
authors: Cathy S
authors: CS
authors: Cutler
authors: The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30332, USA.
authors: Younan
authors: Y
authors: Xia
authors: Department of Radiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors: Yongjian
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D001345:Autoradiography; D045744:Cell Line, Tumor; D005260:Female; D006046:Gold / Q000737:chemistry*; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000556:secondary; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry; D014018:Tissue Distribution / Q000187:drug effects; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D014057:Tomography, X-Ray Computed
chemical_list: D010455:Peptides; D011092:Polyethylene Glycols; D006046:Gold
keywords: CCR5 targeting; breast cancer; gold nanoparticles; radiolabeling; single photon emission computed tomography