title: Peritoneal Washings After Power Morcellation in Laparoscopic Myomectomy: A Pilot Study.
abstract: To evaluate if peritoneal washings of the abdominopelvic cavity during laparoscopic myomectomy can detect leiomyoma cells after power morcellation.
  
  
  
  Prospective cohort pilot study.
  
  
  
  University of North Carolina Hospitals, an academic, tertiary referral center (Canadian Task Force classification II-2).
  
  
  
  Patients undergoing laparoscopic or robotic myomectomy for suspected benign leiomyoma by members of the Minimally Invasive Gynecologic Surgery division between September 2014 and January 2015.
  
  
  
  Washings of the peritoneal cavity were collected at 3 times during surgery: the beginning of the procedure once the peritoneal cavity was accessed laparoscopically, after the myoma was excised and myometrial incision closed, and after uncontained power morcellation.
  
  
  
  Twenty patients were included in the analysis. The median morcellation time was 16 minutes (range, 2-36). The median specimen weight was 283.5 g (range, 13-935). Cytologic evaluation (ThinPrep with Papanicolaou staining) did not detect any smooth muscle cells. Cell block histology, however, detected spindle cells in 6 postmorcellation samples. Three of these 6 cases also had spindle cells detected on the postmyomectomy closure samples. When performed on the postmorcellation samples, desmin and smooth muscle actin immunostaining were positive, confirming the presence of smooth muscle cells.
  
  
  
  Cell block histology, but not cytology, can detect leiomyoma cells in peritoneal washings after power morcellation. With myomectomy, there is some tissue disruption that seems to cause cell spread even in the absence of morcellation. Further protocol testing might allow peritoneal washings to be used in assessing containment techniques and testing comparative safety of different morcellation methods.
authors: Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina. Electronic address: tarek.toubia@gmail.com.
authors: Tarek
authors: T
authors: Toubia
authors: Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina.
authors: Janelle K
authors: JK
authors: Moulder
authors: Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina.
authors: Lauren D
authors: LD
authors: Schiff
authors: Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina.
authors: Daniel
authors: D
authors: Clarke-Pearson
authors: Department of Pathology, University of North Carolina, Chapel Hill, North Carolina.
authors: Siobhan M
authors: SM
authors: O'Connor
authors: Department of Obstetrics and Gynecology, University of North Carolina, Chapel Hill, North Carolina.
authors: Matthew T
authors: MT
authors: Siedhoff
mesh_terms: D034841:Abdominal Cavity / Q000601:surgery*; D000328:Adult; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D007889:Leiomyoma / Q000473:pathology / Q000601:surgery*; D008875:Middle Aged; D019060:Minimally Invasive Surgical Procedures; D000069577:Morcellation / Q000009:adverse effects; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D010529:Peritoneal Cavity / Q000601:surgery*; D010865:Pilot Projects; D011446:Prospective Studies; D007507:Therapeutic Irrigation; D063186:Uterine Myomectomy / Q000379:methods*; D014594:Uterine Neoplasms / Q000473:pathology / Q000601:surgery*
keywords: Laparoscopic myomectomy; Minimally invasive surgery; Morcellation; Pelvic washings; Peritoneal washings; Power morcellation