title: Predicative Factors for Corneal Endothelial Cell Migration.
abstract: OBJECTIVE
  To characterize the effects of Descemet's stripping, Rho-associated protein kinase inhibitor Y-27632, and donor age on endothelial migration in human corneas maintained in ex vivo culture.
  
  
  METHODS
  Twenty-eight cadaveric human corneas underwent ex vivo culture in either standard or Y-27632-supplemented culture medium for 14 days. The posterior surface of each cornea was manipulated to create two types of wounds: scratched wound--corneal endothelial cells (CECs) were denuded from the Descemet's membrane (DM) to leave behind a bare but intact DM; and peeled wound--both the DM and overlying CECs were stripped to leave behind bare corneal stroma. Endothelial migration was assessed via Trypan blue staining. Morphologic traits of CECs were assessed via Alizarin red microscopy and scanning electron microscopy.
  
  
  RESULTS
  The CECs migrated preferentially over scratched wounds compared with peeled wounds. Y-27632 supplementation accelerated endothelial migration over scratched wounds. Endothelial migration decreased with advanced donor age for both wound types, regardless of exposure to Y-27632. Y-27632 supplementation resulted in a less rapid decline in endothelial migration for donors older than 50 years of age for scratched surfaces. Greater cell density and hexagonality was observed over scratched wounds compared with peeled wounds, regardless of Y-27632 supplementation.
  
  
  CONCLUSIONS
  The presence of an intact DM, Y-27632 supplementation, and young donor age are factors that promote endothelial migration in an ex vivo human cornea culture model. The negative effect of age on endothelial migration can be mitigated by the presence of an intact DM and Y-27632 supplementation.
authors: Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore 2Singapore National Eye Centre, Singapore.
authors: Yu Qiang
authors: YQ
authors: Soh
authors: Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore 3Ophthalmology Academic Clinical Program, Duke-National University of Singapore Graduate Medical School, Singapore.
authors: Gary
authors: G
authors: Peh
authors: Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.
authors: Benjamin Lawrence
authors: BL
authors: George
authors: Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.
authors: Xin Yi
authors: XY
authors: Seah
authors: Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.
authors: Nishal Kishinchand
authors: NK
authors: Primalani
authors: Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore.
authors: Khadijah
authors: K
authors: Adnan
authors: Tissue Engineering and Stem Cell Group Singapore Eye Research Institute, Singapore 2Singapore National Eye Centre, Singapore 3Ophthalmology Academic Clinical Program, Duke-National University of Singapore Graduate Medical School, Singapore 4Department of.
authors: Jodhbir Singh
authors: JS
authors: Mehta
mesh_terms: D000328:Adult; D000367:Age Factors; D000577:Amides / Q000494:pharmacology; D000880:Anthraquinones / Q000008:administration & dosage; D002452:Cell Count; D002465:Cell Movement / Q000502:physiology*; D048430:Cell Shape; D004396:Coloring Agents / Q000008:administration & dosage; D003470:Culture Media; D003886:Descemet Membrane / Q000601:surgery; D004728:Endothelium, Corneal / Q000166:cytology* / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D009924:Organ Culture Techniques; D011725:Pyridines / Q000494:pharmacology; D013194:Staining and Labeling; D014019:Tissue Donors; D014343:Trypan Blue; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors
chemical_list: D000577:Amides; D000880:Anthraquinones; D004396:Coloring Agents; D003470:Culture Media; D004791:Enzyme Inhibitors; D011725:Pyridines; C108830:Y 27632; C010078:alizarin; D054460:rho-Associated Kinases; D014343:Trypan Blue