title: An integrated practical implementation of continuous aqueous two-phase systems for the recovery of human IgG: From the microdevice to a multistage bench-scale mixer-settler device.
abstract: Aqueous two-phase systems (ATPS) are a liquid-liquid extraction technology with clear process benefits; however, its lack of industrial embracement is still a challenge to overcome. Antibodies are a potential product to be recovered by ATPS in a commercial context. The objective of this work is to present a more integral approach of the different isolated strategies that have arisen in order to enable a practical, generic implementation of ATPS, using human immunoglobulin G (IgG) as experimental model. A microfluidic device is used for ATPS parameters preselection for product recovery. ATPS were continuously operated in a mixer-settler device in one stage, multistage and multistage with recirculation configuration. Single-stage pure IgG extraction with a polyethylene glycol (PEG) 3350-phophates ATPS within continuous operation allowed a 65% recovery. Further implementation of a multistage platform promoted a higher particle partitioning reaching a 90% recovery. The processing of IgG from a cell supernatant culture harvest in a multistage system with top phase recirculation resulted in 78% IgG recovery in bottom phase. This work conjugates three not widely spread methodologies for ATPS: microfluidics, continuous and multistage operation.
authors: Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico.
authors: Edith
authors: E
authors: Espitia-Saloma
authors: Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico.
authors: Patricia
authors: P
authors: Vâzquez-Villegas
authors: Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico.
authors: Marco
authors: M
authors: Rito-Palomares
authors: Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Campus Monterrey, Monterrey, NL, Mexico. alex.aguilar@itesm.mx.
authors: Oscar
authors: O
authors: Aguilar
mesh_terms: D004867:Equipment Design; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D059625:Liquid-Liquid Extraction / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D007074:Immunoglobulin G; D011092:Polyethylene Glycols
keywords: Continuous aqueous two-phase system; Immunoglobulin G; Microfluidic screening; Mixer-settler device; Multistage extraction