title: Hepatitis B Virus Infection of a Mouse Hepatic Cell Line Reconstituted with Human Sodium Taurocholate Cotransporting Polypeptide.
abstract: Hepatitis B virus (HBV) enters hepatocytes via its receptor, human sodium taurocholate cotransporting polypeptide (hNTCP). So far, HBV infection has been achieved only in human hepatic cells reconstituted with hNTCP and not in cells of mouse origin. Here, the first mouse liver cell line (AML12) which gains susceptibility to HBV upon hNTCP expression is described. Thus, HBV infection of receptor-expressing mouse hepatocytes does not principally require a human cofactor but can be triggered by endogenous murine determinants.
authors: Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany.
authors: Florian A
authors: FA
authors: Lempp
authors: Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany.
authors: Bingqian
authors: B
authors: Qu
authors: Key Laboratory of Medical Molecular Virology, Institute of Medical Microbiology, Shanghai Medical College, Fudan University, Shanghai, China.
authors: Yong-Xiang
authors: YX
authors: Wang
authors: Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany Stephan.Urban@med.uni-heidelberg.de.|German Center for Infection Research (DZIF), Heidelberg, Germany.
authors: Stephan
authors: S
authors: Urban
mesh_terms: D000818:Animals; D002460:Cell Line; D004198:Disease Susceptibility; D015870:Gene Expression; D006509:Hepatitis B / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D006515:Hepatitis B virus / Q000502:physiology*; D003698:Hepatitis Delta Virus / Q000502:physiology; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D051379:Mice; D029363:Organic Anion Transporters, Sodium-Dependent / Q000235:genetics / Q000378:metabolism*; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D029363:Organic Anion Transporters, Sodium-Dependent; D027981:Symporters; C071908:sodium-bile acid cotransporter