title: Quality of histone modification antibodies undermines chromatin biology research.
abstract: Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.
authors: Institute of Biochemistry, Stuttgart University, Stuttgart, 70569, Germany.
authors: Goran
authors: G
authors: Kungulovski
authors: Institute of Biochemistry, Stuttgart University, Stuttgart, 70569, Germany.
authors: Albert
authors: A
authors: Jeltsch
keywords: antibodies; histone modifications; quality control; recombinant proteins