title: Microbulbifer rhizosphaerae sp. nov., isolated from the rhizosphere of the halophyte Arthrocnemum macrostachyum.
abstract: A novel, salt-dependent, non-motile, rod-shaped, Gram-stain-negative and non-endospore-forming bacterium, designated strain Cs16bT, was isolated from the rhizosphere of Arthrocnemum macrostachyum, a halophytic plant at the Lebrija marshes (Seville, Spain). Strain Cs16bT was catalase- and oxidase-positive, and able to hydrolyse casein. Growth occurred from 15-40 °C, at pH 6.0-10.0 and with 1-6% (w/v) NaCl. Q-8 was identified as the major ubiquinone and the predominant cellular fatty acids were iso-C15:0, iso-C17:1cis8, iso-C11:0 3-OH, iso-C17:0, C17:0 cyclo and iso-C11:0. The polar lipids profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two unknown glycophospholipids, an unknown aminoglycophospholipid, an unknown aminophospholipid and an unknown phospholipid. The 16S rRNA gene of strain Cs16bT showed 98.1%, 97.8%, and 97.6% sequence similarity with Microbulbifer maritimus CIP 108504T, Microbulbifer taiwanensis DSM 24146T and Microbulbifer gwangyangensis JCM 17800T, respectively. Based on the phenotypic and genotypic features, it is concluded that strain Cs16bT represents a novel species of the genus Microbulbifer, for which the name Microbulbifer rhizosphaerae sp. nov. is proposed. The type strain is Cs16bT (=DSM 28920T=CECT 8799T).
authors: IFAPA-Instituto de Investigación y Formación Agraria y Pesquera, Centro Las Torres-Tomejil, Ctra. Sevilla-Cazalla de la Sierra, Km 12.2, 41200 Alcalá del Río, Sevilla, Spain.
authors: Maria
authors: M
authors: Camacho
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.|School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors: Maria
authors: M
authors: Del Carmen Montero-Calasanz
authors: Facultad de Biología, Universidad de Sevilla. Avda. Reina Mercedes s/n, ., Sevilla, Spain.
authors: Susana
authors: S
authors: Redondo-Gómez
authors: Facultad de Farmacia, Universidad de Sevilla. Profesor García-González 2, ., Sevilla, Spain.
authors: Ignacio
authors: I
authors: Rodríguez-Llorente
authors: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors: Peter
authors: P
authors: Schumann
authors: School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne, NE1 7RU, UK.
authors: Hans-Peter
authors: HP
authors: Klenk
mesh_terms: D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D019663:Chenopodiaceae / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D055051:Salt-Tolerant Plants / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013030:Spain; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8