title: Evaluation of anti-inflammatory and analgesic activities of extracts from herb of Chelidonium majus L.
abstract: The aim of the study was to evaluate analgesic activity ("hot plate" test), anti-inflammatory activity (carrageenan-induced paw edema) and locomotor activity in rats under the influence of three fractions of Chelidonium majus herb extract: full water extract (FWE), protein enriched fraction (PEF), and non-protein fraction (NPF). Effects of the fractions on the level of chosen cytokines and their mRNA levels were also assessed using lipopolysaccharide (LPS) administration as a proinflammatory cue. All fractions and diclofenac did not affect the locomotor activity of rats in comparison with the control group. FWE and PEF three hours after administration showed statistically significant analgesic activities comparable to morphine (p < 0.05). A slight reduction in rat paw edema was observed after three (comparable with diclofenac) and six hours in the NPF group. FWE revealed a statistically significant pro-inflammatory effect after three hours in comparison with the control group. Peripheral IL-1 and IL-4 cytokine concentrations were reduced under FWE and NPF, PEF fractions. The combination of FWE, PEF and NPF together with LPS showed only the effects of LPS. We suggest that protein enriched fraction (PEF) produced centrally mediated (morphine-like) analgesic action, whereas the anti-inflammatory potential was shown only after LPS-induced inflammation. The precise mechanisms involved in the production of anti-nociceptive and anti-inflammatory responses of studied fractions are not completely understood, but they may be caused rather by the presence of protein more than alkaloids-enriched fraction. This fraction of the extract could be used as an alternative therapy for the prevention of inflammatory-related diseases in the future, but further studies are needed.
authors: Department of Pharmacology, Poznan University of Medical Sciences, Poznan, Poland; Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland.
authors: Przemysław Łukasz
authors: PŁ
authors: Mikołajczak
authors: Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland.
authors: Bogdan
authors: B
authors: Kędzia
authors: Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland; Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Poznan, Poland.
authors: Marcin
authors: M
authors: Ożarowski
authors: Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland.
authors: Radosław
authors: R
authors: Kujawski
authors: Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Poznan, Poland.
authors: Anna
authors: A
authors: Bogacz
authors: Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Poznan, Poland.
authors: Joanna
authors: J
authors: Bartkowiak-Wieczorek
authors: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poznan, Poland.
authors: Wojciech
authors: W
authors: Białas
authors: Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland.
authors: Agnieszka
authors: A
authors: Gryszczyńska
authors: Department of Botany, Breeding and Agricultural Technology for Medicinal Plants, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland.
authors: Waldemar
authors: W
authors: Buchwald
authors: Department of Pharmacology, Poznan University of Medical Sciences, Poznan, Poland.
authors: Michał
authors: M
authors: Szulc
authors: Department of Pharmacology, Poznan University of Medical Sciences, Poznan, Poland.
authors: Natalia
authors: N
authors: Wasiak
authors: Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Poznan, Poland.
authors: Małgorzata
authors: M
authors: Górska-Paukszta
authors: Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland.
authors: Justyna
authors: J
authors: Baraniak
authors: Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Poznan, Poland; Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Szczecin, Poland.
authors: Bogusław
authors: B
authors: Czerny
authors: Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland; Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences, Poznan, Poland; Laboratory of Molecular Biology, Poznan University of Medical Sciences, Poznan, Poland.
authors: Agnieszka
authors: A
authors: Seremak-Mrozikiewicz
keywords: Chelidonium majus aqueous extract; LPS–induced rats; analgesic; anti-inflammatory; mRNA; non-protein fraction; proinflammatory cytokines; protein enriched fraction