title: AN ODORANT-BINDING PROTEIN INVOLVED IN PERCEPTION OF HOST PLANT ODORANTS IN LOCUST Locusta migratoria.
abstract: Locusts, Locusta migratoria (Orthoptera: Acrididae), are extremely destructive agricultural pests, but very little is known of their molecular aspects of perception to host plant odorants including related odorant-binding proteins (OBPs), though several OBPs have been identified in locust. To elucidate the function of LmigOBP1, the first OBP identified from locust, RNA interference was employed in this study to silence LmigOBP1, which was achieved by injection of dsRNA targeting LmigOBP1 into the hemolymph of male nymphs. Compared with LmigOBP1 normal nymphs, LmigOBP1 knockdown nymphs significantly decreased food (maize leaf, Zea mays) consumption and electro-antennography responses to five maize leaf volatiles, ((Z)-3-hexenol, linalool, nonanal, decanal, and (Z)-3-hexenyl acetate). These suggest that LmigOBP1 is involved in perception of host plant odorants.
authors: Plant Protection College, Shenyang Agricultural University, Shenyang, China.
authors: Jia
authors: J
authors: Li
authors: Key Lab for Biological Control, China Agricultural University, Beijing, China.
authors: Long
authors: L
authors: Zhang
authors: Plant Protection College, Shenyang Agricultural University, Shenyang, China.
authors: Xiaoqi
authors: X
authors: Wang
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology*; D006458:Hemolymph / Q000378:metabolism; D060434:Herbivory; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D048188:Locusta migratoria / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008297:Male; D009758:Nymph / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D009812:Odorants; D018515:Plant Leaves / Q000502:physiology; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D003313:Zea mays / Q000502:physiology
chemical_list: D019476:Insect Proteins; D012330:RNA, Double-Stranded; D018035:Receptors, Odorant; C045648:odorant-binding protein
keywords: EAG; Locusta migratoria; RNAi; chemoreception; host odorants; odorant-binding protein