title: CD10-positive myoepithelial cells are usually prominent around in situ lobular neoplasia of the breast and much less prominent or absent in DCIS.
abstract: OBJECTIVE
  To study the relationship between the neoplastic cells of in situ lobular neoplasia (ILN) and ductal carcinoma in situ (DCIS) and the surrounding CD10-positive myoepithelial cells.
  
  
  METHODS
  Twenty consecutive cases of ILN and 51 of DCIS were stained for CD10 using the immunoperoxidase technique. The presence of CD10-positive cells was assessed semiquantitatively on a scale of 0-3 where 0 indicates their absence and 3 indicates the presence of multiple layers, which can be focal.
  
  
  RESULTS
  Ninety per cent of ILN cases scored 3, compared with none of DCIS (p=0.0001). There was a significant relationship between DCIS grade and CD10 score, with the mean scores being 1.43, 0.82 and 0.5 for low, intermediate and high grade, respectively. CD10-positive cells were always present around low-grade DCIS, but absent in 27% of high-grade cases. CD10-positive cells were more frequent in ER-positive than in ER-negative DCIS, and in HER2-negative than in HER2-positive cases, but the difference was not statistically significant.
  
  
  CONCLUSIONS
  There is a distinct relationship between the type and grade of in situ neoplasia of the breast and the surrounding CD10-positive myoepithelial cells, suggesting the presence of a 'cross talk' between the two elements.
authors: Department of Histopathology, Charing Cross Hospital, Imperial College Healthcare NHS Trust and Imperial College, London, UK.
authors: Sami
authors: S
authors: Shousha
authors: Department of Histopathology, Charing Cross Hospital, Imperial College Healthcare NHS Trust and Imperial College, London, UK.
authors: Georgina
authors: G
authors: Forbes
authors: Department of Histopathology, Charing Cross Hospital, Imperial College Healthcare NHS Trust and Imperial College, London, UK.
authors: Ilona
authors: I
authors: Hopkins
authors: Department of Histopathology, Charing Cross Hospital, Imperial College Healthcare NHS Trust and Imperial College, London, UK.
authors: Josephine Anne
authors: JA
authors: Wright
mesh_terms: D014408:Biomarkers, Tumor; D001940:Breast / Q000378:metabolism / Q000473:pathology*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism / Q000473:pathology*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D015260:Neprilysin / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D015260:Neprilysin
keywords: BREAST; BREAST PATHOLOGY; IMMUNOHISTOCHEMISTRY