title: Formation mechanism and biological activity of novel thiolated human-like collagen iron complex.
abstract: To develop an iron supplement that is effectively absorbed and utilized, thiolated human-like collagen was created to improve the iron binding capacity of human-like collagen. A thiolated human-like collagen-iron complex was prepared in a phosphate buffer, and one mole of thiolated human-like collagen-iron possessed approximately 28.83 moles of iron. The characteristics of thiolated human-like collagen-iron were investigated by ultraviolet-visible absorption spectroscopy, Fourier transform infrared spectroscopy, circular dichroism, and differential scanning calorimetry. The results showed that the thiolated human-like collagen-iron complex retained the secondary structure of human-like collagen and had greater thermodynamic stability than human-like collagen, although interactions between iron ions and human-like collagen occurred during the formation of the complex. In addition, to evaluate the bioavailability of thiolated human-like collagen-iron, an in vitro Caco-2 cell model and an in vivo iron deficiency anemia mouse model were employed. The data demonstrated that the thiolated human-like collagen-iron complex exhibited greater bioavailability and was more easily utilized than FeSO4, ferric ammonium citrate, or ferrous glycinate. These results indicated that the thiolated human-like collagen-iron complex is a potential iron supplement in the biomedical field.
authors: Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, China Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Xi'an, China.
authors: Chenhui
authors: C
authors: Zhu
authors: Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, China Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Xi'an, China.
authors: Lingyun
authors: L
authors: Liu
authors: Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, China Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Xi'an, China.
authors: Jianjun
authors: J
authors: Deng
authors: Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, China Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Xi'an, China.
authors: Xiaoxuan
authors: X
authors: Ma
authors: Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, China Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Xi'an, China.
authors: Junfeng
authors: J
authors: Hui
authors: Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, China Shaanxi R&D Center of Biomaterials and Fermentation Engineering, Northwest University, Xi'an, China fandaidi@nwu.edu.cn.
authors: Daidi
authors: D
authors: Fan
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D001682:Biological Availability; D018938:Caco-2 Cells; D003094:Collagen / Q000737:chemistry* / Q000493:pharmacokinetics / Q000627:therapeutic use*; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D007501:Iron / Q000737:chemistry* / Q000493:pharmacokinetics / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D013438:Sulfhydryl Compounds / Q000737:chemistry* / Q000493:pharmacokinetics / Q000627:therapeutic use*
chemical_list: D056831:Coordination Complexes; D013438:Sulfhydryl Compounds; D003094:Collagen; D007501:Iron
keywords: Caco-2 cell; Human-like collagen; bioavailability; iron; mouse model