title: In vitro stress effect on degradation and drug release behaviors of basic fibroblast growth factor--poly(lactic-co-glycolic-acid) microsphere.
abstract: OBJECTIVE
  To study the degradation and basic fibroblast growth factor (bFGF) release activity of bFGF - poly(lactic-co-glycolic-acid) microsphere (bFGF-PLGA MS) under stress in vitro, including the static pressure and shearing force-simulating mechanical environment of the joint cavity.
  
  
  METHODS
  First, bFGF-PLGA MSs were created. Meanwhile, two self-made experimental instruments (static pressure and shearing force loading instruments) were initially explored to provide stress-simulating mechanical environment of the joint cavity. Then, bFGF-PLGA MSs were loaded into the two instruments respectively, to study microsphere degradation and drug release experiments. In the static pressure loading experiment, normal atmospheric pressure loading (approximately 0.1 MPa), 0.35 MPa, and 4.0 MPa pressure loading and shaking flask oscillation groups were designed to study bFGF-PLGA MS degradation and bFGF release. In the shearing force loading experiment, a pulsating pump was used to give the experimental group an output of 1,000 mL/min and the control group an output of 10 mL/min to carry out bFGF-PLGA MS degradation and drug release experiments. Changes of bFGF-PLGA MSs, including microsphere morphology, quality, weight-average molecular weight of polymer, and microsphere degradation and bFGF release, were analyzed respectively.
  
  
  RESULTS
  In the static pressure loading experiment, bFGF-PLGA MSs at different pressure were stable initially. The trend of molecular weight change, quality loss, and bFGF release was consistent. Meanwhile, microsphere degradation and bFGF release rates in the 4.0 MPa pressure loading group were faster than those in the normal and 0.35 MPa pressure loading groups. It was the fastest in the shaking flask group, showing a statistically significant difference (P<0.0001). In the shearing force loading experiment, there were no distinctive differences in the rates of microsphere degradation and bFGF release between experimental and control group. Meanwhile, microsphere degradation and bFGF release rates by shaking flask oscillation were obviously faster than those by shearing force only (P<0.0001).
  
  
  CONCLUSIONS
  There are significant effects on bFGF-PLGA MS degradation and bFGF release due to the interaction between extraction stress and time. Static pressure has a conspicuous influence on bFGF-PLGA MS degradation and release, especially at a pressure of 4.0 MPa. The shearing force has a slight effect on bFGF-PLGA MS degradation and drug release. On the contrary, shaking flask oscillation has a significantly distinctive effect.
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Yan
authors: Y
authors: Xiong
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Zeping
authors: Z
authors: Yu
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Yun
authors: Y
authors: Lang
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Juanyu
authors: J
authors: Hu
authors: Laboratory of Biomechanical Engineering, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Hong
authors: H
authors: Li
authors: Laboratory of Biomechanical Engineering, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Yonggang
authors: Y
authors: Yan
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Chongqi
authors: C
authors: Tu
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Tianfu
authors: T
authors: Yang
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Yueming
authors: Y
authors: Song
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Hong
authors: H
authors: Duan
authors: Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Fuxing
authors: F
authors: Pei
mesh_terms: D001274:Atmospheric Pressure; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry; D007596:Joints / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry*; D008863:Microspheres; D008970:Molecular Weight; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011312:Pressure; D013997:Time Factors
chemical_list: D004337:Drug Carriers; D016222:Fibroblast Growth Factor 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: PLGA; bFGF; basic fibroblast growth factor; drug release; microsphere; poly(lactic-co-glycolic-acid); stress