title: Single-Molecule Stochastic Analysis of Channeling Enzyme Tryptophan Synthase.
abstract: The channeling enzyme tryptophan synthase provides a paradigmatic example of a chemical nanomachine. It possesses two active centers and, as a single molecule, catalyzes 13 different reaction steps with a complex pattern of allosteric regulation and with an intermediate product channeled from one active center to another. Here, the first single-molecule stochastic model of the enzyme is proposed and analyzed. All its transition rate constants were deduced from the experimental data available, and no fitting parameters were thus employed. Numerical simulations reveal strong correlations in the states of the active centers and the emergent synchronization of intramolecular processes in tryptophan synthase.
authors: Department of Physical Chemistry, Fritz Haber Institute of the Max Planck Society , 14195 Berlin, Germany.
authors: Dimitri
authors: D
authors: Loutchko
authors: Unité de Chronobiologie théorique, Faculté des Sciences, Université Libre de Bruxelles , 1050 Brussels, Belgium.
authors: Didier
authors: D
authors: Gonze
authors: Department of Physical Chemistry, Fritz Haber Institute of the Max Planck Society , 14195 Berlin, Germany.|Department of Mathematical and Life Sciences, Hiroshima University , 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan.
authors: Alexander S
authors: AS
authors: Mikhailov
mesh_terms: D002151:Calorimetry; D007700:Kinetics; D008958:Models, Molecular; D013269:Stochastic Processes; D014367:Tryptophan Synthase / Q000737:chemistry*
chemical_list: D014367:Tryptophan Synthase