title: Effects of long-term folate supplementation on metabolic status and regression of cervical intraepithelial neoplasia: A randomized, double-blind, placebo-controlled trial.
abstract: OBJECTIVE
  This study was conducted to determine the effects of long-term folate supplementation on regression and metabolic status of patients with cervical intraepithelial neoplasia grade 1 (CIN1).
  
  
  METHODS
  This randomized, double-blind, placebo-controlled trial was performed among 58 women diagnosed with CIN1, ages 18 to 55 y old. Participants were randomly divided into two groups to receive 5 mg/d folate supplements (n = 29) or placebo (n = 29) for 6 mo. Fasting blood samples were taken at baseline and 6 mo after intervention to quantify related markers.
  
  
  RESULTS
  A greater percentage of women in the folate group had regressed CIN1 (83.3 versus 52.0%, P = 0.019) than those in the placebo group. Long-term folate supplementation resulted in a significant decrease in serum insulin levels (-1.6 ± 6.2 versus +2.6 ± 6.9 μIU/mL, P = 0.018) and homeostatic model assessment-beta cell function (HOMA-B) (-13.0 ± 39.0 versus +11.2 ± 42.3, P = 0.028) compared with the placebo. Additionally, plasma glutathione (GSH) levels were significantly increased (+81.5 ± 264.1 versus -220.9 ± 342.5 μmol/L, P < 0.001) and malondialdehyde (MDA) levels were significantly reduced (-1.0 ± 1.1 versus +0.1 ± 1.6 μmol/L, P = 0.004) in the folate group compared to the placebo.
  
  
  CONCLUSIONS
  Taken together, folate supplementation (5 mg/d) for 6 mo among women with CIN1 resulted in its regression as well as led to decreased serum insulin, HOMA-B, plasma MDA and increased plasma GSH levels; however, it did not affect other metabolic profiles.
authors: Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R. Iran.
authors: Zatollah
authors: Z
authors: Asemi
authors: Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran.
authors: Zahra
authors: Z
authors: Vahedpoor
authors: Endocrinology and Metabolism Research Center, Arak University of Medical Sciences, Arak, Iran; Department of Gynecology and Obstetrics, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
authors: Mehri
authors: M
authors: Jamilian
authors: Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R. Iran.
authors: Fereshteh
authors: F
authors: Bahmani
authors: Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: esmaillzadeh@hlth.mui.ac.ir.
authors: Ahmad
authors: A
authors: Esmaillzadeh
mesh_terms: D000328:Adult; D001786:Blood Glucose; D002097:C-Reactive Protein; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D005492:Folic Acid / Q000494:pharmacology*; D005500:Follow-Up Studies; D006801:Humans; D007328:Insulin; D007333:Insulin Resistance / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D013995:Time; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D014803:Vitamin B Complex / Q000494:pharmacology; D002578:Uterine Cervical Dysplasia / Q000188:drug therapy* / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D014803:Vitamin B Complex; D002097:C-Reactive Protein; D005492:Folic Acid
keywords: Cervical intraepithelial neoplasia; Folate supplementation; Metabolic profiles; Regression