title: Water-Transfer Slows Aging in Saccharomyces cerevisiae.
abstract: Transferring Saccharomyces cerevisiae cells to water is known to extend their lifespan. However, it is unclear whether this lifespan extension is due to slowing the aging process or merely keeping old yeast alive. Here we show that in water-transferred yeast, the toxicity of polyQ proteins is decreased and the aging biomarker 47Q aggregates at a reduced rate and to a lesser extent. These beneficial effects of water-transfer could not be reproduced by diluting the growth medium and depended on de novo protein synthesis and proteasomes levels. Interestingly, we found that upon water-transfer 27 proteins are downregulated, 4 proteins are upregulated and 81 proteins change their intracellular localization, hinting at an active genetic program enabling the lifespan extension. Furthermore, the aging-related deterioration of the heat shock response (HSR), the unfolded protein response (UPR) and the endoplasmic reticulum-associated protein degradation (ERAD), was largely prevented in water-transferred yeast, as the activities of these proteostatic network pathways remained nearly as robust as in young yeast. The characteristics of young yeast that are actively maintained upon water-transfer indicate that the extended lifespan is the outcome of slowing the rate of the aging process.
authors: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors: Aviv
authors: A
authors: Cohen
authors: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors: Esther
authors: E
authors: Weindling
authors: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors: Efrat
authors: E
authors: Rabinovich
authors: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors: Iftach
authors: I
authors: Nachman
authors: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors: Shai
authors: S
authors: Fuchs
authors: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors: Silvia
authors: S
authors: Chuartzman
authors: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors: Lihi
authors: L
authors: Gal
authors: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors: Maya
authors: M
authors: Schuldiner
authors: Department of Biochemistry and Molecular Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors: Shoshana
authors: S
authors: Bar-Nun
mesh_terms: D002455:Cell Division; D003470:Culture Media / Q000494:pharmacology; D060746:Endoplasmic Reticulum-Associated Degradation; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000378:metabolism; D009172:Mycology / Q000379:methods; D010455:Peptides / Q000032:analysis; D066329:Protein Aggregates; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D056811:Unfolded Protein Response; D014867:Water
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D010455:Peptides; D066329:Protein Aggregates; D014867:Water; C097188:polyglutamine