title: Simultaneous Quantitative Detection of Helicobacter Pylori Based on a Rapid and Sensitive Testing Platform using Quantum Dots-Labeled Immunochromatiographic Test Strips.
abstract: Quantum dots-labeled urea-enzyme antibody-based rapid immunochromatographic test strips have been developed as quantitative fluorescence point-of-care tests (POCTs) to detect helicobacter pylori. Presented in this study is a new test strip reader designed to run on tablet personal computers (PCs), which is portable for outdoor detection even without an alternating current (AC) power supply. A Wi-Fi module was integrated into the reader to improve its portability. Patient information was loaded by a barcode scanner, and an application designed to run on tablet PCs was developed to handle the acquired images. A vision algorithm called Kmeans was used for picture processing. Different concentrations of various human blood samples were tested to evaluate the stability and accuracy of the fabricated device. Results demonstrate that the reader can provide an easy, rapid, simultaneous, quantitative detection for helicobacter pylori. The proposed test strip reader has a lighter weight than existing detection readers, and it can run for long durations without an AC power supply, thus verifying that it possesses advantages for outdoor detection. Given its fast detection speed and high accuracy, the proposed reader combined with quantum dots-labeled test strips is suitable for POCTs and owns great potential in applications such as screening patients with infection of helicobacter pylori, etc. in near future.
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. alvinshmtuzxy@163.com.
authors: Yu
authors: Y
authors: Zheng
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. wk_xa@163.com.
authors: Kan
authors: K
authors: Wang
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. zhangjingjingdlu@sina.cn.
authors: Jingjing
authors: J
authors: Zhang
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. 1602701580@qq.com.
authors: Weijian
authors: W
authors: Qin
authors: The Outpatient Department of Zhujiang Hosptial, South Medical University, 1023 South Shatai Road, Guangzhou, 510515, People's Republic of China. 911492059@qq.com.
authors: Xinyu
authors: X
authors: Yan
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. gxshen@sjtu.edu.cn.
authors: Guangxia
authors: G
authors: Shen
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. guogao@sjtu.edu.cn.
authors: Guo
authors: G
authors: Gao
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. feipan@sjtu.edu.cn.
authors: Fei
authors: F
authors: Pan
authors: Institute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China. dxcui@sjtu.edu.cn.
authors: Daxiang
authors: D
authors: Cui
keywords: CMOS; Kmeans algorithm; QD test strips; Test strip reader; Urea enzymes; Wi-Fi transmission