title: Hsa-miR-326 targets CCND1 and inhibits non-small cell lung cancer development.
abstract: Hsa-miRNA-326 (miR-326) has recently been discovered having anticancer efficacy in different organs. However, the role of miR-326 on non-small cell lung cancer (NSCLC) is still ambiguous. In this study, we investigated the role of miR-326 on the development of NSCLC. The results indicated that miR-326 was significantly down-regulated in primary tumor tissues and very low levels were found in NSCLC cell lines. Ectopic expression of miR-326 in NSCLC cell lines significantly suppressed cell growth as evidenced by cell viability assay, colony formation assay and BrdU staining, through inhibition of cyclin D1, cyclin D2, CDK4 and up-regulation of p57(Kip2) and p21(Waf1/Cip1). In addition, miR-326 induced apoptosis, as indicated by concomitantly with up-regulation of key apoptosis protein cleaved caspase-3, and down-regulation of anti-apoptosis protein Bcl2. Moreover, miR-326 inhibited cellular migration and invasiveness through inhibition of matrix metalloproteinases (MMP)-7 and MMP-9. Further, oncogene CCND1 was revealed to be a putative target of miR-326, which was inversely correlated with miR-326 expression in NSCLC. Taken together, our results demonstrated that miR-326 played a pivotal role on NSCLC through inhibiting cell proliferation, migration, invasion, and promoting apoptosis by targeting oncogenic CCND1.
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.
authors: Chengcao
authors: C
authors: Sun
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.|Department of Pharmacology, Basic Medical School, Nanyang Medical College, 473003 Nanyang, P.R.China.
authors: Chuanfeng
authors: C
authors: Huang
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.|Wuhan Hospital for The Prevention and Treatment of Occupational Diseases, 430071 Wuhan, P.R.China.
authors: Shujun
authors: S
authors: Li
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.
authors: Cuili
authors: C
authors: Yang
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.
authors: Yongyong
authors: Y
authors: Xi
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.
authors: Liang
authors: L
authors: Wang
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.
authors: Feng
authors: F
authors: Zhang
authors: The Third Xiang-Ya Hospital, Central South University, 410013 Changsha, P.R.China.
authors: Yunfeng
authors: Y
authors: Fu
authors: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, 430071 Wuhan, P.R.China.
authors: Dejia
authors: D
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C530104:CCND1 protein, human; C545322:MIRN326 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D019938:Cyclin D1
keywords: Hsa-miRNA-326 (miR-326); apoptosis; cyclin D1; non-small cell lung cancer (NSCLC); proliferation