title: A simple and rapid HPLC-DAD method for simultaneously monitoring the accumulation of alkaloids and precursors in different parts and different developmental stages of Catharanthus roseus plants.
abstract: A rapid and simple reversed phase liquid chromatographic system has been developed for simultaneous analysis of terpenoid indole alkaloids (TIAs) and their precursors. This method allowed separation of 11 compounds consisting of eight TIAs (ajmalicine, serpentine, catharanthine, vindoline, vindolinine, vincristine, vinblastine, and anhydrovinblastine) and three related precursors i.e., tryptophan, tryptamine and loganin. The system has been applied for screening the TIAs and precursors in Catharanthus roseus plant extracts. In this study, different organs i.e., flowers, leaves, stems, and roots of C. roseus were investigated. The results indicate that TIAs and precursor accumulation varies qualitatively and quantitatively in different organs of C. roseus. The precursors showed much lower levels than TIAs in all organs. Leaves and flowers accumulate higher level of vindoline, catharanthine and anhydrovinblastine while roots have higher level of ajmalicine, vindolinine and serpentine. Moreover, the alkaloid profiles of leaves harvested at different ages and different growth stages were studied. The results show that the levels of monoindole alkaloids decreased while bisindole alkaloids increased with leaf aging and upon plant growth. The HPLC method has been successfully applied to detect TIAs and precursors in different types of C. roseus samples to facilitate further study of the TIA pathway and its regulation in C. roseus plants.
authors: Plant Biotechnology Research Center, SJTU-Cornell Institute of Sustainable Agriculture and Biotechnology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, PR China. Electronic address: panqf@sjtu.edu.cn.
authors: Qifang
authors: Q
authors: Pan
authors: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors: Mohd Zuwairi
authors: MZ
authors: Saiman
authors: Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.
authors: Natali Rianika
authors: NR
authors: Mustafa
authors: Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333 BE, The Netherlands.
authors: Robert
authors: R
authors: Verpoorte
authors: Plant Biotechnology Research Center, SJTU-Cornell Institute of Sustainable Agriculture and Biotechnology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, PR China.
authors: Kexuan
authors: K
authors: Tang
mesh_terms: D030008:Catharanthus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D046948:Secologanin Tryptamine Alkaloids / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D046948:Secologanin Tryptamine Alkaloids
keywords: Alkaloids precursors; Catharanthus roseus; HPLC-DAD; Simultaneous; Terpenoid indole alkaloids