title: RNA interference screening of interferon-stimulated genes with antiviral activities against classical swine fever virus using a reporter virus.
abstract: Classical swine fever (CSF) caused by classical swine fever virus (CSFV) is a highly contagious and often fatal disease of pigs, which leads to significant economic losses in many countries. Viral infection can induce the production of interferons (IFNs), giving rise to the transcription of hundreds of IFN-stimulated genes (ISGs) to exert antiviral effects. Although numerous ISGs have been identified to possess antiviral activities against different viruses, rare anti-CSFV ISGs have been reported to date. In this study, to screen anti-CSFV ISGs, twenty-one ISGs reported previously were individually knocked down using small interfering RNAs (siRNAs) followed by infection with a reporter CSFV expressing Renilla luciferase (Rluc). As a result, four novel anti-CSFV ISGs were identified, including natural-resistance-associated macrophage protein 1 (NRAMP1), cytosolic 5'-nucleotidase III A (NT5C3A), chemokine C-X-C motif ligand 10 (CXCL10), and 2'-5'-oligoadenylate synthetase 1 (OAS1), which were further verified to exhibit antiviral activities against wild-type CSFV. We conclude that the reporter virus is a useful tool for efficient screening anti-CSFV ISGs.
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Xiao
authors: X
authors: Wang
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Yongfeng
authors: Y
authors: Li
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Lian-Feng
authors: LF
authors: Li
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Liang
authors: L
authors: Shen
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Lingkai
authors: L
authors: Zhang
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Jiahui
authors: J
authors: Yu
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Yuzi
authors: Y
authors: Luo
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Yuan
authors: Y
authors: Sun
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China.
authors: Su
authors: S
authors: Li
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Harbin 150001, PR China. Electronic address: huajiqiu@hvri.ac.cn.
authors: Hua-Ji
authors: HJ
authors: Qiu
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000032:analysis / Q000235:genetics / Q000276:immunology; D000818:Animals; D027682:Cation Transport Proteins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D054357:Chemokine CXCL10 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006691:Classical Swine Fever / Q000235:genetics* / Q000276:immunology*; D006692:Classical Swine Fever Virus / Q000502:physiology*; D005786:Gene Expression Regulation; D007372:Interferons / Q000276:immunology*; D049410:Luciferases, Renilla / Q000032:analysis / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D013552:Swine; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D015088:2',5'-Oligoadenylate Synthetase; D027682:Cation Transport Proteins; D054357:Chemokine CXCL10; D007372:Interferons; D049410:Luciferases, Renilla; D034741:RNA, Small Interfering; D000099173:Natural Resistance-associated Macrophage Protein 1
keywords: Antiviral activities; Classical swine fever virus; Interferon stimulated genes; Reporter virus; Small interfering RNAs