title: The importance of pyridoxine for the impact of the dietary selenium sources on redox balance, embryo development, and reproductive performance in gilts.
abstract: This study aimed to determine the effects of dietary pyridoxine and selenium (Se) on embryo development, reproductive performance and redox system in gilts. Eighty-four gilts were fed one of five diets: CONT) basal diet; MSeB60) CONT+0.3mg/kg of Na-selenite; MSeB610) diet 2+10mg/kg of HCl-pyridoxine; OSeB60) CONT+0.3mg/kg of Se-enriched yeast; and OSeB610) diet 4+10mg/kg of HCl-pyridoxine. Blood samples were collected for long-term (each estrus and slaughter) and peri-estrus (fourth estrus d -4 to d +3) profiles. At slaughter (gestation d 30), organs and embryos were collected. For long-term and peri-estrus profiles, Se level and source affected (P<0.01) blood Se concentration whereas B6 level increased (P<0.01) erythrocyte pyridoxal-5-phosphate concentration. A B6 level (P<0.05) effect was observed on long-term plasma Se-dependent glutathione peroxidase (Se-GPX) activity whereas peri-estrus Se-GPX was minimum on d -1 (P<0.01). Selenium level increased sows' organs and embryo Se concentration (P<0.01). Selenium source tended to enhance embryo Se content (P=0.06). Within-litter embryo Se content was increased by B6 level (P<0.01). Selenium level tended to affect Se-GPX and total GPX activities in organs mitochondria (P=0.09 and 0.07, respectively). Selenium source affected kidney ATP synthesis (P=0.05). In conclusion, B6 level affected the Se-GPX activity on a long-term basis, whereas the basal level of Se was adequate during the peri-estrus period. Embryo quality was not improved by dietary Se, and B6 impaired within-litter homogeneity.
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada; Department of Animal Science, Universidade Estadual de Londrina, Londrina, Paraná 86057-970, Brazil. Electronic address: danyel.buenodalto@agr.gc.ca.
authors: Danyel Bueno
authors: DB
authors: Dalto
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada. Electronic address: isabelle.audet@agr.gc.ca.
authors: Isabelle
authors: I
authors: Audet
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada. Electronic address: jerome.lapointe@agr.gc.ca.
authors: Jérôme
authors: J
authors: Lapointe
authors: Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada. Electronic address: jacques.matte@agr.gc.ca.
authors: J Jacques
authors: JJ
authors: Matte
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D011247:Pregnancy; D011736:Pyridoxine / Q000627:therapeutic use*; D012098:Reproduction / Q000187:drug effects*; D012643:Selenium / Q000627:therapeutic use*; D013552:Swine
chemical_list: D012643:Selenium; D011736:Pyridoxine
keywords: embryo; gestation; glutathione peroxidase; pig; pyridoxine; selenium