title: [The Effect of the Chromophore Charge on the Efficiency of Incorporation of Fluorescently-labeled Nucleotides in Matrix Synthesis by Taq DNA Polymerase].
abstract: In order to study the effect of an electrical charge of the chromophore, on the efficiency of incorporation of fluorescently-labeled nucleotides into DNA during PCR, three fluorescently-labeled dUPT, one of which with electroneutral and other two with positively and negatively charged dyes (Cy5 analogs), were synthesized. It is shown that dUPT, labeled with electroneutral Cy 5 analog, is most effectively incorporated into DNA when Tag polymerase is used for PCR.
authors: V E
authors: VE
authors: Shershov
authors: V E
authors: VE
authors: Kuznetsova
authors: Yu P
authors: YP
authors: Lysov
authors: T O
authors: TO
authors: Guseinov
authors: V E
authors: VE
authors: Barsky
authors: M A
authors: MA
authors: Spitsyn
authors: O A
authors: OA
authors: Zasedateleva
authors: V A
authors: VA
authors: Vasiliskov
authors: S A
authors: SA
authors: Surzhikov
authors: A S
authors: AS
authors: Zasedatelev
authors: A V
authors: AV
authors: Chudinov
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA; D019914:Taq Polymerase