title: IGF-1 C Domain-Modified Hydrogel Enhances Cell Therapy for AKI.
abstract: Low cell retention and engraftment after transplantation limit the successful application of stem cell therapy for AKI. Engineered microenvironments consisting of a hydrogel matrix and growth factors have been increasingly successful in controlling stem cell fate by mimicking native stem cell niche components. Here, we synthesized a bioactive hydrogel by immobilizing the C domain peptide of IGF-1 (IGF-1C) on chitosan, and we hypothesized that this hydrogel could provide a favorable niche for adipose-derived mesenchymal stem cells (ADSCs) and thereby enhance cell survival in an AKI model. In vitro studies demonstrated that compared with no hydrogel or chitosan hydrogel only, the chitosan-IGF-1C hydrogel increased cell viability through paracrine effects. In vivo, cotransplantation of the chitosan-IGF-1C hydrogel and ADSCs in ischemic kidneys ameliorated renal function, likely by the observed promotion of stem cell survival and angiogenesis, as visualized by bioluminescence imaging and attenuation of fibrosis. In conclusion, IGF-1C immobilized on a chitosan hydrogel provides an artificial microenvironment for ADSCs and may be a promising therapeutic approach for AKI.
authors: School of Medicine and State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China; Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China; and.
authors: Guowei
authors: G
authors: Feng
authors: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China;
authors: Jimin
authors: J
authors: Zhang
authors: School of Medicine and.
authors: Yang
authors: Y
authors: Li
authors: School of Medicine and.
authors: Yan
authors: Y
authors: Nie
authors: School of Medicine and.
authors: Dashuai
authors: D
authors: Zhu
authors: School of Medicine and.
authors: Ran
authors: R
authors: Wang
authors: Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, China.
authors: Jianfeng
authors: J
authors: Liu
authors: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China;
authors: Jie
authors: J
authors: Gao
authors: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China;
authors: Na
authors: N
authors: Liu
authors: School of Medicine and.
authors: Ningning
authors: N
authors: He
authors: School of Medicine and.
authors: Wei
authors: W
authors: Du
authors: School of Medicine and.
authors: Hongyan
authors: H
authors: Tao
authors: School of Medicine and.
authors: Yongzhe
authors: Y
authors: Che
authors: Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China; and zongjinli@nankai.edu.cn qiangzhao@nankai.edu.cn xuyong8816@126.com.
authors: Yong
authors: Y
authors: Xu
authors: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China;
authors: Deling
authors: D
authors: Kong
authors: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China; zongjinli@nankai.edu.cn qiangzhao@nankai.edu.cn xuyong8816@126.com.
authors: Qiang
authors: Q
authors: Zhao
authors: School of Medicine and State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China; zongjinli@nankai.edu.cn qiangzhao@nankai.edu.cn xuyong8816@126.com.
authors: Zongjin
authors: Z
authors: Li
mesh_terms: D058186:Acute Kidney Injury / Q000628:therapy*; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D048271:Chitosan; D003131:Combined Modality Therapy; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007334:Insulin-Like Growth Factor I; D048271:Chitosan
keywords: acute renal failure; apoptosis; cell survival; stem cell; survival; transplantation