title: Oleanane-type triterpene saponins from Hydrocotyle nepalensis.
abstract: Five new oleanane-type triterpene saponins including steganogenin 3-O-β-D-glucopyranoside (1), steganogenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (2), steganogenin 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside (3), chichipegenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (4) and chichipegenin 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside (5), along with four known oleanane-type triterpenes and triterpene saponins (6-9) were isolated from EtOH extract of the whole plant of Hydrocotyle nepalensis. To the best of our knowledge, oleanane-type triterpenes possessing skeleton with 17, 22-seco-backbone (1-3) are not common in natural products. Compound 8 was isolated as a new natural product. The structures of new compounds were elucidated by extensive spectroscopic methods and chemical evidence. Moreover, the cytotoxic activity of all the isolates against five selected human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7 and SW480) was evaluated and the results indicated that compounds 6 and 7 show stronger cytotoxic activity.
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China; University of the Chinese Academy of Sciences, Beijing 100039, P. R. China.
authors: Rui-Jing
authors: RJ
authors: Ma
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China; University of the Chinese Academy of Sciences, Beijing 100039, P. R. China.
authors: Zhen-Hua
authors: ZH
authors: Liu
authors: Longrun Pu-er Tea Academy, Yunnan Agricultural University, Kunming 650201, P. R. China.
authors: Cheng-Ting
authors: CT
authors: Zi
authors: College of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230038, P. R. China.
authors: Wei
authors: W
authors: Gao
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China; University of the Chinese Academy of Sciences, Beijing 100039, P. R. China.
authors: Fa-Wu
authors: FW
authors: Dong
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China.
authors: Liu
authors: L
authors: Yang
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China; University of the Chinese Academy of Sciences, Beijing 100039, P. R. China.
authors: Jin-Yu
authors: JY
authors: Li
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China.
authors: Jun
authors: J
authors: Zhou
authors: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, P.R. China. Electronic address: hujiangmiao@mail.kib.ac.cn.
authors: Jiang-Miao
authors: JM
authors: Hu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor / Q000187:drug effects; D028041:Centella / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry* / Q000302:isolation & purification; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D009828:Oleanolic Acid
keywords: Cytotoxic activity; Hydrocotyle nepalensis; Oleanane-type triterpene saponin