title: [Application of Whole-cell Biosensor ADP1_pWHlux for Acute Toxicity Detection in Water Environment].
abstract: A whole-cell biosensor acinetobacter ADP1_pWHlux was constructed by genetic engineering for detecting acute toxicity, so as to overcome the harsh application conditions when detecting acute toxicity using natural luminescent bacteria or whole-cell biosensor constructed by model microorganisms as the host cell. Detection methods, detection sensitivity and detection range of acinetobacter ADP1_pWHlux were studied. The results showed that the luminescence of ADP1_pWHlux was inhibited by acute poison, poison dose and inhibition of luminescence exhibit dose-response relationship. ADPL_pWHlux was respond to 4 mg x L(-1) HgCl2 within 5 min. The detection limit for HgCl2 was 0.04 mg x L(-1). The detectable effects for indicators of Be2+, Ba2+, Cu2+, Ni2+ in standards for drinking water quality were obvious. The detection range of Be2+, Ba2+, Cu2+ were 0.025-250 mg x L(-1), the detection range of Ni2+, was 0.0025-250 mg x L(-1), the detection limit of Pb2+, BrO3(-) , ClO2(-) were 0.002 5 mg x L(-1), the detection limit of ClO3(-) was 0.025 mg x L(-1). The whole-cell biosensor ADPl_pWHlux detection method has been applied to evaluate acute toxicity in water environment of Qinghe river in Beijing, indicating the established method can be used to detect contaminated water samples.
authors: Hui
authors: H
authors: Tang
authors: Yi-zhi
authors: YZ
authors: Song
authors: Bo
authors: B
authors: Jiang
authors: Guang-yu
authors: GY
authors: Chen
authors: Jian-li
authors: JL
authors: Jia
authors: Xu
authors: X
authors: Zhang
authors: Guang-he
authors: GH
authors: Li
mesh_terms: D000150:Acinetobacter; D000068476:Beijing; D015374:Biosensing Techniques; D057230:Limit of Detection; D049449:Luminescence; D045483:Rivers; D023382:Toxicity Tests, Acute; D014877:Water Pollution, Chemical / Q000032:analysis*; D060753:Water Quality