title: RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking.
abstract: Glycerol is a key yeast metabolite in winemaking because it contributes to improve the organoleptic properties of wine. It is also a cellular protective molecule that enhances the tolerance of yeasts to osmotic stress and promotes longevity. Thus, its production increases by genetic manipulation, which is of biotechnological and basic interest. Glycerol is produced by diverting glycolytic glyceraldehyde-3-phosphate through the action of glycerol-3-phosphate dehydrogenase (coded by genes GPD1 and GPD2). Here, we demonstrate that RNA-binding protein Pub1p regulates glycerol production by controlling Gpd1p activity. Its deletion does not alter GPD1 mRNA levels, but protein levels and enzymatic activity increase, which explains the higher intracellular glycerol concentration and greater tolerance to osmotic stress of the pub1∆ mutant. PUB1 deletion also enhances the activity of nicotinamidase, a longevity-promoting enzyme. Both enzymatic activities are partially located in peroxisomes, and we detected peroxisome formation during wine fermentation. The role of Pub1p in life span control depends on nutrient conditions and is related with the TOR pathway, and a major connection between RNA metabolism and the nutrient signaling response is established.
authors: Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain. helena.orozco@uv.es.|Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain. helena.orozco@uv.es.
authors: Helena
authors: H
authors: Orozco
authors: Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain.|Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.
authors: Ana
authors: A
authors: Sepúlveda
authors: Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain.|Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.
authors: Cecilia
authors: C
authors: Picazo
authors: Departament de Bioquímica i Biologia Molecular, Universitat de València, Valencia, Spain.|Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.
authors: Emilia
authors: E
authors: Matallana
authors: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, IATA-CSIC, Avda. Agustín Escardino, 7, 46980, Paterna, Spain.
authors: Agustín
authors: A
authors: Aranda
mesh_terms: D005285:Fermentation; D005511:Food Handling; D000067030:Fruit and Vegetable Juices / Q000382:microbiology; D015966:Gene Expression Regulation, Fungal; D005990:Glycerol / Q000378:metabolism*; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009997:Osmotic Pressure; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D039102:Poly(A)-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological; D027843:Vitis / Q000382:microbiology; D014920:Wine / Q000032:analysis* / Q000382:microbiology
chemical_list: D005990:Glycerol; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D005993:Glycerolphosphate Dehydrogenase; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D019869:Phosphatidylinositol 3-Kinases; D039102:Poly(A)-Binding Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C093263:FPS1 protein, S cerevisiae; C083327:PUB1 protein, S cerevisiae; C470555:STL1 protein, S cerevisiae; C568531:GPD1 protein, S cerevisiae; C568532:GPD2 protein, S cerevisiae; C083324:TOR1 protein, S cerevisiae; C530964:SCH9 protein, S cerevisiae
keywords: Glycerol; Gpd1p; Peroxisomes; Pub1p; Saccharomyces cerevisiae; TOR1/Sch9 nutrient signaling pathway; Winemaking