title: Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags.
abstract: Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies.
authors: Centre for Personalized NanoMedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Roads (Bldg 75), Brisbane, QLD 4072, Australia. y.wang27@uq.edu.au m.trau@uq.edu.au.
authors: Yuling
authors: Y
authors: Wang
authors: Eugene J H
authors: EJ
authors: Wee
authors: Matt
authors: M
authors: Trau
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019175:DNA Methylation; D023281:Genomics; D057230:Limit of Detection; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology