title: A novel type of self-assembled nanoparticles as targeted gene carriers: an application for plasmid DNA and antimicroRNA oligonucleotide delivery.
abstract: In this study, a new type of amphiphilic cetylated polyethyleneimine (PEI) was synthesized, and then polylactic-co-glycolic acid (PLGA)/cetylated PEI/hyaluronic acid nanoparticles (PCPH NPs) were developed by self-assembly as a novel type of gene-delivering vehicle. The PCPH NPs showed good DNA-condensation ability by forming polyplexes with small particle size and positive zeta potential. The transfection efficiency and cytotoxicity of PCPH NPs were evaluated as plasmid DNA vectors to transfect HepG2 in vitro. PCPH NPs exhibited much lower cytotoxicity and higher gene-transfection efficiency than PEI (25,000) and commercial transfection reagents. Furthermore, PCPH NPs were used as an anti-miR-221 vector for transfecting HepG2 cells, and anti-miR-221 was effectively transfected into cells and produced a greater inhibitory effect on cancer-cell growth by PCPH NPs. These results demonstrate that PCPH NPs can be a promising nonviral vector for gene-delivery systems.
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.
authors: Yanliang
authors: Y
authors: Zhu
authors: School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, People's Republic of China.
authors: Gaofeng
authors: G
authors: Liang
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.
authors: Bo
authors: B
authors: Sun
authors: Department of Neurobiology, Nanjing Medical University, Nanjing, People's Republic of China.
authors: Tian
authors: T
authors: Tian
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.
authors: Feihu
authors: F
authors: Hu
authors: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, People's Republic of China.
authors: Zhongdang
authors: Z
authors: Xiao
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004337:Drug Carriers; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D056945:Hep G2 Cells; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D004247:DNA; D004337:Drug Carriers; D019344:Lactic Acid; D035683:MicroRNAs; D009841:Oligonucleotides; D011094:Polyethyleneimine; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C525709:MIR221, human
keywords: PLGA; anti-miR-221; gene delivery; hyaluronic acid; polyethyleneimine