title: Multi-platform metabolomics analyses of a broad collection of fragrant and non-fragrant rice varieties reveals the high complexity of grain quality characteristics.
abstract: The quality of rice in terms not only of its nutritional value but also in terms of its aroma and flavour is becoming increasingly important in modern rice breeding where global targets are focused on both yield stabilityandgrain quality. In the present paper we have exploited advanced, multi-platform metabolomics approaches to determine the biochemical differences in 31 rice varieties from a diverse range of genetic backgrounds and origin. All were grown under the specific local conditions for which they have been bred and all aspects of varietal identification and sample purity have been guaranteed by local experts from each country. Metabolomics analyses using 6 platforms have revealed the extent of biochemical differences (and similarities) between the chosen rice genotypes. Comparison of fragrant rice varieties showed a difference in the metabolic profiles of jasmine and basmati varieties. However with no consistent separation of the germplasm class. Storage of grains had a significant effect on the metabolome of both basmati and jasmine rice varieties but changes were different for the two rice types. This shows how metabolic changes may help prove a causal relationship with developing good quality in basmati rice or incurring quality loss in jasmine rice in aged grains. Such metabolomics approaches are leading to hypotheses on the potential links between grain quality attributes, biochemical composition and genotype in the context of breeding for improvement. With this knowledge we shall establish a stronger, evidence-based foundation upon which to build targeted strategies to support breeders in their quest for improved rice varieties.
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.|Centre for BioSystems Genomics, P.O. Box 98, 6700 AB Wageningen, The Netherlands.
authors: R
authors: R
authors: Mumm
authors: Biometris-Applied Statistics, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.
authors: J A
authors: JA
authors: Hageman
authors: Grain Quality, and Nutrition Centre, International Rice Research Institute, DAPO 7777, Metro Manila, Philippines.|Laboratory of Plant Physiology, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.|School of Agriculture and Food Science, University of Queensland, St Lucia, QLD 4072 Australia.
authors: M N
authors: MN
authors: Calingacion
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.|Centre for BioSystems Genomics, P.O. Box 98, 6700 AB Wageningen, The Netherlands.|Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors: R C H
authors: RCH
authors: de Vos
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.|Centre for BioSystems Genomics, P.O. Box 98, 6700 AB Wageningen, The Netherlands.
authors: H H
authors: HH
authors: Jonker
authors: Max-Planck-Institute of Molecular Plant Physiology (MPIMP), Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
authors: A
authors: A
authors: Erban
authors: Max-Planck-Institute of Molecular Plant Physiology (MPIMP), Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
authors: J
authors: J
authors: Kopka
authors: Plant and Soil Science Section, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen (UC), Thorvaldsensvej 40, 1871 Frederiksberg C Copenhagen, Denmark.
authors: T H
authors: TH
authors: Hansen
authors: Plant and Soil Science Section, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen (UC), Thorvaldsensvej 40, 1871 Frederiksberg C Copenhagen, Denmark.
authors: K H
authors: KH
authors: Laursen
authors: Plant and Soil Science Section, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen (UC), Thorvaldsensvej 40, 1871 Frederiksberg C Copenhagen, Denmark.
authors: J K
authors: JK
authors: Schjoerring
authors: The National Centre for Plant and Microbial Metabolomics, Rothamsted Research, West Common, Harpenden, Herts AL52JQ UK.
authors: J L
authors: JL
authors: Ward
authors: The National Centre for Plant and Microbial Metabolomics, Rothamsted Research, West Common, Harpenden, Herts AL52JQ UK.
authors: M H
authors: MH
authors: Beale
authors: Ubonratchathani Rice Research Centre, Ubon Ratchathani, Thailand.
authors: S
authors: S
authors: Jongee
authors: Rice Programme, National Agricultural Research Centre, Islamabad, Pakistan.
authors: A
authors: A
authors: Rauf
authors: Grain Quality Division, Rice Research Institute of Iran (RRII), Km 5 Tehran Rd, 41996-13475 Rasht, Islamic Republic of Iran.
authors: F
authors: F
authors: Habibi
authors: Indonesian Center for Rice Research (ICRR) BB Padi, Jl. Raya 9, Sukamandi, Subang, 41256 Jawa Barat Indonesia.
authors: S D
authors: SD
authors: Indrasari
authors: Cambodian Agricultural Research and Development Institute, CARDI Rd, Phnom Penh, Cambodia.
authors: S
authors: S
authors: Sakhan
authors: Pusat Penyelidikan Padi dan Tanaman Industri, MARDI, Seberang Perai Beg Berkunci 203 Pejabat Pos Kepala Batas, 13200 Seberang Perai Pulau, Penang Malaysia.
authors: A
authors: A
authors: Ramli
authors: Rice Chemistry and Food Science Division, Philippine Rice Research Institute, Maligaya, Science City of Muñoz, 3119 Nueva Ecija Philippines.
authors: M
authors: M
authors: Romero
authors: Graham Centre for Agricultural Innovation, Agricultural Institute (An Alliance Between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga, NSW Australia.|Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO 7777, Metro Manila, Philippines.
authors: R F
authors: RF
authors: Reinke
authors: Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata, Japan.
authors: K
authors: K
authors: Ohtsubo
authors: Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002 Thailand.|Rice and Cash Crops Research Centre, National Agriculture and Forestry Research Institute, PDR, Vientiane, Lao.
authors: C
authors: C
authors: Boualaphanh
authors: Grain Quality, and Nutrition Centre, International Rice Research Institute, DAPO 7777, Metro Manila, Philippines.|School of Agriculture and Food Science, University of Queensland, St Lucia, QLD 4072 Australia.
authors: M A
authors: MA
authors: Fitzgerald
authors: Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.|Centre for BioSystems Genomics, P.O. Box 98, 6700 AB Wageningen, The Netherlands.|Laboratory of Plant Physiology, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen, The Netherlands.|Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors: R D
authors: RD
authors: Hall
keywords: Basmati; Grain quality; Jasmine; Multi-platform analysis; PLS-DA; Rice