title: Comparative In Vitro Binding Studies of TiCl2(dpme)2, Ti(ada)2(bzac)2, and TiCl2(bzac)(bpme) Titanium Complexes with Calf-Thymus DNA.
abstract: The binding of TiCl2(dpme)2 (1), (dpme = 6,6'-dimethyl-2,2'-bipyridine), Ti(ada)2(bzac)2 (2), (ada = adamantylamine; bzac = benzoylacetone), and TiCl2(bzac)(bpme) (3), (bpme = 4,4'-dimethyl-2,2'-bipyrdine) with calf thymus (ct) DNA has been studied by UV-visible spectroscopy, thermal denaturation, and circular dichroism spectroscopy. In UV-visible study complexes 1, 2, and 3 showed red, blue, and red shifts, respectively, upon the addition of ct-DNA along with a significant hyperchromism. The intrinsic binding constants (K b ) calculated from UV-visible absorption studies were 2.3 × 10(3) M(-1), 3.3 × 10(3) M(-1) and, 7.1 × 10(3) M(-1) for complexes 1, 2, and 3, respectively. The change in melting temperature (ΔT m ) was calculated to be 2-3°C for each complex. Circular dichroism (CD) study showed blue shift for complex 2 and red shift for complexes 1 and 3 along with rise in molecular ellipticity upon the addition of complexes. Results suggest a binding mode of complex 2 different than 1 and 3.
authors: Department of Chemistry, National Institute of Technology, Hamirpur, Himachal Pradesh 177005, India.
authors: Pamita
authors: P
authors: Awasthi
authors: Department of Chemistry, National Institute of Technology, Hamirpur, Himachal Pradesh 177005, India.
authors: Nitesh
authors: N
authors: Kumar
authors: Department of Chemistry, National Institute of Technology, Hamirpur, Himachal Pradesh 177005, India.
authors: Raj
authors: R
authors: Kaushal
authors: Department of Chemistry North Campus, University of Delhi, Delhi 110007, India.
authors: Mohan
authors: M
authors: Kumar
authors: Department of Chemistry North Campus, University of Delhi, Delhi 110007, India.
authors: Shrikant
authors: S
authors: Kukreti