title: Glycopeptide-functionalized gold nanoparticles for antibody induction against the tumor associated mucin-1 glycoprotein.
abstract: We report the preparation of gold nanoparticle (AuNP)-based vaccine candidates against the tumor-associated form of the mucin-1 (MUC1) glycoprotein. Chimeric peptides, consisting of a glycopeptide sequence derived from MUC1 and the T-cell epitope P30 sequence were immobilized on PEGylated AuNPs and the ability to induce selective antibodies in vivo was investigated. After immunization, mice showed significant MHC-II mediated immune responses and their antisera recognized human MCF-7 breast cancer cells. Nanoparticles designed according to this report may become key players in the development of anticancer vaccines.
authors: Gesellschaft zur Förderung der Analytischen Wissenschaften e.V. ISAS-Leibniz Institute for Analytical Sciences, Otto-Hahn-Str. 6b, 44227 Dortmund, Germany.
authors: Hui
authors: H
authors: Cai
authors: Center for Biomolecular Nanotechnologies@UniLe-Istituto Italiano di Tecnologia (IIT), Via Barsanti, 73010 Arnesano, Lecce, Italy.
authors: Federica
authors: F
authors: Degliangeli
authors: Institute of Organic Chemistry, Johannes Gutenberg University of Mainz, Duesbergweg 10-14, 55128 Mainz, Germany.
authors: Björn
authors: B
authors: Palitzsch
authors: University Medical Center, Institute of Immunology, Johannes Gutenberg University of Mainz, Langenbeckstr. 1, Geb. 708, 55101 Mainz, Germany.
authors: Bastian
authors: B
authors: Gerlitzki
authors: Institute of Organic Chemistry, Johannes Gutenberg University of Mainz, Duesbergweg 10-14, 55128 Mainz, Germany.
authors: Horst
authors: H
authors: Kunz
authors: University Medical Center, Institute of Immunology, Johannes Gutenberg University of Mainz, Langenbeckstr. 1, Geb. 708, 55101 Mainz, Germany. Electronic address: eschmitt@uni-mainz.de.
authors: Edgar
authors: E
authors: Schmitt
authors: Center for Biomolecular Nanotechnologies@UniLe-Istituto Italiano di Tecnologia (IIT), Via Barsanti, 73010 Arnesano, Lecce, Italy. Electronic address: roberto.fiammengo@iit.it.
authors: Roberto
authors: R
authors: Fiammengo
authors: Gesellschaft zur Förderung der Analytischen Wissenschaften e.V. ISAS-Leibniz Institute for Analytical Sciences, Otto-Hahn-Str. 6b, 44227 Dortmund, Germany. Electronic address: ulrika.westerlind@isas.de.
authors: Ulrika
authors: U
authors: Westerlind
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019496:Cancer Vaccines / Q000737:chemistry / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D005802:Genes, MHC Class II; D006020:Glycopeptides / Q000737:chemistry / Q000276:immunology*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007114:Immunization; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000737:chemistry / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology / Q000517:prevention & control
chemical_list: D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D006020:Glycopeptides; D018396:Mucin-1; D006046:Gold
keywords: Antibodies; Cancer; Glycopeptides; Nanoparticles; Vaccines