title: Analysis of IL-10 and IL-6 Gene Polymorphisms and Their Serum Levels in Patients with Brucellosis: A Case Control Study.
abstract: BACKGROUND
  It seems that polymorphism in the regulatory areas of cytokine genes affects the cytokine production capacity and may play a role in the development of infectious diseases. Interleukin-10 (IL-10) and Interleukin-6 (IL-6), which are cytokines of Th2, cause the macrophage become inactive and patient conditions get worse.
  
  
  METHODS
  In this case-control study, 60 patients with brucellosis and 60 healthy participants were recruited. IL-10 genotyping at positions -1082 (G/A), -819 (C/T), and -592 (C/A) and IL-6 genotyping at position -174 (G/C) were analyzed by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) methods. The levels of IL-10 and IL-6 were determined by a sandwich enzyme-linked immunosorbent assay in sera of study population.
  
  
  RESULTS
  The AA and CC genotypes of the IL-10 gene at positions -1082 G/A and -819 C/T were significantly more frequent in patients in comparison to controls, respectively. The AG genotype of the IL-10 gene at positions -1082 G/A was significantly more frequent in control groups than the patients. Serum levels of IL-10 and IL-6 were significantly more frequent in the patients than in the control groups.
  
  
  CONCLUSIONS
  Our study showed that the AA and CC genotypes at positions -1082 and -819 are very important, respectively. These results suggest that IL-10 (-1082 G/A) GG genotype may be considered as a risk factor for brucellosis, while the AG genotype might be a protective factor against the disease.
authors: a Department of Microbiology, Faculty of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
authors: Sima
authors: S
authors: Kazemi
authors: b Research Center for Molecular Medicine, School of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
authors: Massoud
authors: M
authors: Saidijam
authors: c Brucellosis Research Center , Hamadan University of Medical Sciences , Hamadan , Iran.
authors: Seyed Hamid
authors: SH
authors: Hashemi
authors: d Department of Biostatistics and Epidemiology , School of Public Health, Hamadan University of Medical Sciences , Hamadan , Iran.
authors: Manoochehr
authors: M
authors: Karami
authors: e Department of Clinical Biochemistry , Kermanshah University of Medical Sciences , Kermanshah , Iran.
authors: Asad
authors: A
authors: Vaisi-Raygani
authors: a Department of Microbiology, Faculty of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.|b Research Center for Molecular Medicine, School of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
authors: Mohammad Yousef
authors: MY
authors: Alikhani
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002006:Brucellosis / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D016753:Interleukin-10 / Q000097:blood* / Q000235:genetics*; D015850:Interleukin-6 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10
keywords: Brucellosis; IL-10; IL-6; case control; polymorphism