title: Tenuigenin Prevents IL-1β-induced Inflammation in Human Osteoarthritis Chondrocytes by Suppressing PI3K/AKT/NF-κB Signaling Pathway.
abstract: Tenuigenin (TEN), the main active component of Polygala tenuifolia, has been reported to have anti-inflammatory effects. However, the effects of TEN on IL-1β-stimulated osteoarthritis chondrocytes have not been reported. The purpose of this study was to investigate the anti-inflammatory effects and mechanism of TEN on IL-1β-stimulated human osteoarthritis chondrocytes. Human osteoarthritis chondrocytes were pretreated with or without TEN for 1 h and then stimulated with IL-1β. The production of NO and PGE2 were detected by the Griess reagent and ELISA. The expression of NF-κB and MAPKs (p38, JNK, ERK) were measured by Western blot analysis. The production of MMP-1, MMP3, and MMP13 were measured by ELISA. The results showed that treatment of TEN significantly inhibited IL-1β-induced NO and PGE2 production. TEN also suppressed IL-1β-induced MMP-1, MMP3, and MMP13 expression. Furthermore, TEN was found to inhibit IL-1β-induced NF-κB activation, PI3K, and AKT phosphorylation. In conclusion, these results suggest that TEN inhibits IL-1β-induced inflammation in human osteoarthritis chondrocytes by inhibiting PI3K/AKT/NF-κB signaling pathway.
authors: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
authors: Chunlei
authors: C
authors: Wang
authors: Harbin Children Hospital, Harbin, 150010, China.
authors: Lihong
authors: L
authors: Zeng
authors: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
authors: Tao
authors: T
authors: Zhang
authors: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China.
authors: Jiakun
authors: J
authors: Liu
authors: Department of Orthopedics, the First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China. wangwenbolb@sina.com.
authors: Wenbo
authors: W
authors: Wang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019645:Arthroplasty, Replacement, Knee; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D015232:Dinoprostone / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000096:biosynthesis; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D053583:Interleukin-1beta / Q000276:immunology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000096:biosynthesis; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis; D053509:Matrix Metalloproteinase 13 / Q000096:biosynthesis; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis; D009569:Nitric Oxide / Q000096:biosynthesis; D010003:Osteoarthritis / Q000188:drug therapy; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; C508585:IL1B protein, human; D053583:Interleukin-1beta; C498143:RELA protein, human; D051996:Transcription Factor RelA; C493220:tenuigenin; D009569:Nitric Oxide; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D015232:Dinoprostone
keywords: IL-1β; NF-κB; PI3K; osteoarthritis chondrocyte; tenuigenin