title: Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles and an alternative pathway with DMSO.
abstract: Proton caged compounds exhibit a characteristic behavior when directly dosed into cells or being coupled to gold nanoparticles prior to the dosing. When irradiated in the near ultraviolet region, they release protons that interact with intracellular HCO3 (-) to yield H2CO3. The dissociation of carbonic acid, then, releases CO2 that can be distinctively singled out in infrared spectra. In the process of searching a pathway to augment the intracellular uptake of proton caged compounds, we probed the association of 1-(2-nitrophenyl)-ethylhexadecyl sulfonate (HDNS) with DMSO, an agent to enhance the membrane permeability. We found out a different UV-induced protonation mechanism that opens up to new conduits of employing of proton caged compounds. Here, we report the infrared data we collected in this set of experiments.
authors: Dept. of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1-00133 Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi, Viale Medaglie d'Oro 305, 00136 Rome, Italy.
authors: Marilena
authors: M
authors: Carbone
authors: Dept. of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1-00133 Rome, Italy; Dept. of Chemistry, University of Rome La Sapienza, P.le A. Moro, 00185 Rome, Italy.
authors: Gianfranco
authors: G
authors: Sabbatella
authors: Dept. of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1-00133 Rome, Italy.
authors: Simonetta
authors: S
authors: Antonaroli
authors: Laboratoire de Chimie Physique, UMR 8000-CNRS, Bât. 349, Université Paris-Sud, 91405 Orsay, France.
authors: Hynd
authors: H
authors: Remita
authors: Dept. of Biology and Biotechnology "Charles Darwin", University of Rome La Sapienza, P.le A. Moro, 00185 Rome, Italy.
authors: Viviana
authors: V
authors: Orlando
authors: Dept. of Biology and Biotechnology "Charles Darwin", University of Rome La Sapienza, P.le A. Moro, 00185 Rome, Italy.
authors: Stefano
authors: S
authors: Biagioni
authors: Dept. of Physics, University of Rome La Sapienza, P.le A. Moro, 00185 Rome, Italy; Center for Life Nano Science, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161 Rome, Italy.
authors: Alessandro
authors: A
authors: Nucara
keywords: DMSO; Intracellular proton release; Proton caged compounds