title: Genome-Wide Identification of Epigenetic Hotspots Potentially Related to Cardiovascular Risk in Adult Women after a Complicated Pregnancy.
abstract: BACKGROUND
  The physiological demands of pregnancy on the maternal cardiovascular system can catapult women into a metabolic syndrome that predisposes to atherosclerosis in later life. We sought to identify the nature of the epigenomic changes associated with the increased cardiovascular disease (CVD) risk in adult women following pre-eclampsia.
  
  
  RESULTS
  We assessed the genome wide epigenetic profile by methyl-C sequencing of monozygotic parous twin sister pairs discordant for a severe variant of pre-eclampsia. In the adult twin sisters at risk for CVD as a consequence of a complicated pregnancy, a set of 12 differentially methylated regions with at least 50% difference in methylation percentage and the same directional change was found to be shared between the affected twin sisters and significantly different compared to their unaffected monozygous sisters.
  
  
  CONCLUSIONS
  The current epigenetic marker set will permit targeted analysis of differentially methylated regions potentially related to CVD risk in large cohorts of adult women following complicated pregnancies.
authors: Department of Clinical Chemistry and Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Cees
authors: C
authors: Oudejans
authors: Department of Clinical Chemistry and Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Ankie
authors: A
authors: Poutsma
authors: Department of Clinical Chemistry and Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Omar
authors: O
authors: Michel
authors: Department of Clinical Chemistry and Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Joyce
authors: J
authors: Mulders
authors: Department of Clinical Chemistry and Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Allerdien
authors: A
authors: Visser
authors: Department of Clinical Chemistry and Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Marie
authors: M
authors: van Dijk
authors: Department of Physiology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.|A-Skin BV, Amsterdam, De Boelelaan 1118, 1081 HZ, Amsterdam, The Netherlands.
authors: Tessa
authors: T
authors: Nauta
authors: Department of Obstetrics/Gynaecology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Anouk
authors: A
authors: Bokslag
authors: Department of Physiology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Walter
authors: W
authors: Paulus
authors: Department of Physiology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Andreas
authors: A
authors: de Haas
authors: Department of Physiology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Pieter
authors: P
authors: Koolwijk
authors: Department of Obstetrics/Gynaecology, VU University Medical Center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.
authors: Christianne J M
authors: CJ
authors: de Groot
mesh_terms: D000328:Adult; D002318:Cardiovascular Diseases / Q000209:etiology / Q000235:genetics* / Q000503:physiopathology; D002319:Cardiovascular System / Q000378:metabolism / Q000503:physiopathology; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D005819:Genetic Markers; D015894:Genome, Human; D055106:Genome-Wide Association Study; D006801:Humans; D008137:Longitudinal Studies; D011225:Pre-Eclampsia / Q000235:genetics* / Q000503:physiopathology; D011247:Pregnancy; D012307:Risk Factors; D012720:Severity of Illness Index; D035781:Siblings; D013997:Time Factors; D014430:Twins, Monozygotic / Q000235:genetics
chemical_list: D005819:Genetic Markers