title: Cannabinoid receptor 2 augments eosinophil responsiveness and aggravates allergen-induced pulmonary inflammation in mice.
abstract: Accumulation of activated eosinophils in tissue is a hallmark of allergic inflammation. The endocannabinoid 2-arachidonoylglycerol (2-AG) has been proposed to elicit eosinophil migration in a CB2 receptor/Gi/o -dependent manner. However, it has been claimed recently that this process may also involve other mechanisms such as cytokine priming and the metabolism of 2-AG into eicosanoids. Here, we explored the direct contribution of specific CB2 receptor activation to human and mouse eosinophil effector function in vitro and in vivo.
  
  
  
  In vitro studies including CB2 expression, adhesion and migratory responsiveness, respiratory burst, degranulation, and calcium mobilization were conducted in human peripheral blood eosinophils and mouse bone marrow-derived eosinophils. Allergic airway inflammation was assessed in mouse models of acute OVA-induced asthma and directed eosinophil migration.
  
  
  
  CB2 expression was significantly higher in eosinophils from symptomatic allergic donors. The selective CB2 receptor agonist JWH-133 induced a moderate migratory response in eosinophils. However, short-term exposure to JWH-133 potently enhanced chemoattractant-induced eosinophil shape change, chemotaxis, CD11b surface expression, and adhesion as well as production of reactive oxygen species. Receptor specificity of the observed effects was confirmed in eosinophils from CB2 knockout mice and by using the selective CB2 antagonist SR144528. Of note, systemic application of JWH-133 clearly primed eosinophil-directed migration in vivo and aggravated both AHR and eosinophil influx into the airways in a CB2 -specific manner. This effect was completely absent in eosinophil-deficient ∆dblGATA mice.
  
  
  
  Our data indicate that CB2 may directly contribute to the pathogenesis of eosinophil-driven diseases. Moreover, we provide new insights into the molecular mechanisms underlying the CB2 -mediated priming of eosinophils. Hence, antagonism of CB2 receptors may represent a novel pharmacological approach for the treatment of allergic inflammation and other eosinophilic disorders.
authors: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors: R B
authors: RB
authors: Frei
authors: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors: P
authors: P
authors: Luschnig
authors: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors: G P
authors: GP
authors: Parzmair
authors: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors: M
authors: M
authors: Peinhaupt
authors: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors: S
authors: S
authors: Schranz
authors: Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: A
authors: A
authors: Fauland
authors: Division of Physiological Chemistry II, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: C E
authors: CE
authors: Wheelock
authors: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors: A
authors: A
authors: Heinemann
authors: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors: E M
authors: EM
authors: Sturm
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D015415:Biomarkers; D002118:Calcium / Q000378:metabolism; D002186:Cannabinoids / Q000494:pharmacology; D015550:Cell Degranulation / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D004804:Eosinophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D037342:Pertussis Toxin / Q000276:immunology; D011014:Pneumonia / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism
chemical_list: D000485:Allergens; D015415:Biomarkers; D002186:Cannabinoids; D043885:Receptor, Cannabinoid, CB2; D037342:Pertussis Toxin; D054460:rho-Associated Kinases; D002118:Calcium; C432747:1,1-dimethylbutyl-1-deoxy-Delta(9)-THC
keywords: Cannabinoid receptor 2; Eosinophils; Ovalbumin-induced asthma; Priming; airway hyperresponsiveness