title: Cardiac-protective effects and the possible mechanisms of alatamine during acute myocardial ischemia.
abstract: Alatamine is a constituent in the extract of a traditional herbal medicine Ramulus euonymi widely used for cardiac protection. However, its precise effects remain unclear. In the present study, we found that alatamine was able to reduce acute myocardial ischemia (AMI)-induced cardiac dysfunction in a rat model, as reflected by significantly restored electrocardiograms, M-mode echocardiograms, and left ventricular hemodynamics. Also, Nagar Olsen staining revealed that alatamine markedly reduced AMI-induced cardiac injury and cardiac myocyte apoptosis. TUNEL and caspase-3 activity assay showed that cardiac myocytes underwent significant apoptosis during AMI, and levels of LDH and CK-MB increased in the serum. However, such changes were significantly inhibited by pre-administration of alatamine. Furthermore, such anti-apoptotic effects of alatamine was also confirmed in a cardiac myocyte model of isoproterenol (ISO)-induced damage. Mechanistically, it was also found that alatamine improved the expression and activity of sarcoplasmic/endoplasmic reticulum Ca(2+) ATPase (SERCA), which were inhibited during AMI, promoting contractility and relaxation. Meanwhile, alatamine decreased Bax and increased Bcl-2 expressions both in vivo and in vitro, therefore inhibiting cardiac myocyte apoptosis and preventing cardiac dysfunction caused by AMI at the cellular level. The present study revealed the beneficial role of alatamine in cardiac protection and highlighted it as a potential therapeutic reagent for reduction of AMI-induced cardiac injury.
authors: Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.|Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.
authors: Chengguo
authors: C
authors: Zhao
authors: Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.|Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.
authors: Fanxin
authors: F
authors: Meng
authors: Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.|Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.
authors: Lulu
authors: L
authors: Geng
authors: Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.|Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.
authors: Xi
authors: X
authors: Zhao
authors: Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.|Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.
authors: Hui
authors: H
authors: Zhou
authors: Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.|Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.
authors: Yao
authors: Y
authors: Zhang
authors: Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.|Department of Chemistry and Pharmacy, Zhuhai College, Jilin University, Zhuhai 519041, P. R. China.
authors: Shuzhi
authors: S
authors: Qin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D052279:Creatine Kinase, MB Form / Q000378:metabolism; D004642:Emodin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006352:Heart Ventricles / Q000187:drug effects* / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism
chemical_list: D020011:Protective Agents; C053054:2-hydroxyemodin; D052279:Creatine Kinase, MB Form; D053148:Caspase 3; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D004642:Emodin; D007545:Isoproterenol
keywords: Ca2+ ATPase du réticulum sarcoplasmique/endoplasmique; Ramulus euonymi; acute myocardial ischemia; alatamine; apoptose; apoptosis; cardiac myocyte; ischémie cardiaque aigüe; myocytes cardiaques; sarcoplasmic/endoplasmic reticulum Ca2+ ATPase