title: Creating Completely Both Male and Female Sterile Plants by Specifically Ablating Microspore and Megaspore Mother Cells.
abstract: Although genetically modified (GM) plants have improved commercially important traits, such as biomass and biofuel production, digestibility, bioremediation, ornamental value, and tolerance to biotic and abiotic stresses, there remain economic, political, or social concerns over potential ecological effects of transgene flow from GM plants. The current solution for preventing transgene flow from GM plants is genetically engineering sterility; however, approaches to generating both male and female sterility are limited. In addition, existing strategies for creating sterility lead to loss or modifications of entire flowers or floral organs. Here, we demonstrate that instead of the 1.5-kb promoter, the entire SOLO DANCERS (SDS) gene is required for its meiocyte-specific expression. We then developed an efficient method to specifically ablate microspore and megaspore mother cells using the SDS and BARNASE fusion gene, which resulted in complete sterility in both male and female reproductive organs in Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum), but did not affect plant growth or development, including the formation of all flower organs. Therefore, our research provides a general and effective tool to prevent transgene flow in GM plants.
authors: Department of Biological Sciences, University of Wisconsin-Milwaukee Milwaukee, WI, USA.
authors: Jian
authors: J
authors: Huang
authors: Department of Biological Sciences, University of Wisconsin-Milwaukee Milwaukee, WI, USA.
authors: Ashley R
authors: AR
authors: Smith
authors: Department of Biological Sciences, University of Wisconsin-Milwaukee Milwaukee, WI, USA.
authors: Tianyu
authors: T
authors: Zhang
authors: Department of Biological Sciences, University of Wisconsin-Milwaukee Milwaukee, WI, USA.
authors: Dazhong
authors: D
authors: Zhao
keywords: SOLO DANCERS; completely both male and female sterile plants; flower structure; gene flow; genetic ablation; microspore and megaspore mother cells