title: MicroRNA transcriptome profiling of mice brains infected with Japanese encephalitis virus by RNA sequencing.
abstract: Japanese encephalitis (JE) is a mosquito borne viral disease, caused by Japanese encephalitis virus (JEV) infection producing severe neuroinflammation in the central nervous system (CNS) with the associated disruption of the blood brain barrier. MicroRNAs (miRNAs) are a family of 21-24 nt small non-coding RNAs that play important post-transcriptional regulatory roles in gene expression and have critical roles in virus pathogenesis. We examined the potential roles of miRNAs in JEV-infected suckling mice brains and found that JEV infection changed miRNA expression profiles when the suckling mice began showing nervous symptoms. A total of 1062 known and 71 novel miRNAs were detected in JEV-infected group, accompanied with 1088 known and 75 novel miRNAs in mock controls. Among these miRNAs, one novel and 25 known miRNAs were significantly differentially expressed, including 18 up-regulated and 8 down-regulated miRNAs which were further confirmed by real-time PCR. Gene ontology (GO) and signaling pathway analysis of the predicted target mRNAs of the modulated miRNAs showed that they are correlated with the regulation of apoptosis, neuron differentiation, antiviral immunity and infiltration of mouse brain, and the validated targets of 12 differentially expressed miRNAs were enriched for the regulation of cell programmed death, proliferation, transcription, muscle organ development, erythrocyte differentiation, gene expression, plasma membrane and protein domain specific binding. KEGG analysis further reveals that the validated target genes were involved in the Pathways in cancer, Neurotrophin signaling pathway, Toll like receptor signaling pathway, Endometrial cancer and Jak-STAT signaling pathway. We constructed the interaction networks of miRNAs and their target genes according to GO terms and KEGG pathways and the expression levels of several target genes were examined. Our data provides a valuable basis for further studies on the regulatory roles of miRNAs in JE pathogenesis.
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors: Xin-Feng
authors: XF
authors: Li
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors: Rui-Bing
authors: RB
authors: Cao
authors: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors: Jun
authors: J
authors: Luo
authors: The Laboratory of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors: Jian-Ming
authors: JM
authors: Fan
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors: Jing-Man
authors: JM
authors: Wang
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors: Yuan-Peng
authors: YP
authors: Zhang
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors: Jin-Yan
authors: JY
authors: Gu
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors: Xiu-Li
authors: XL
authors: Feng
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors: Bin
authors: B
authors: Zhou
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: puyanchennj@yeah.net.
authors: Pu-Yan
authors: PY
authors: Chen
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D002460:Cell Line; D019295:Computational Biology / Q000379:methods; D004195:Disease Models, Animal; D004664:Encephalitis Virus, Japanese; D004672:Encephalitis, Japanese / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: Brain; Japanese encephalitis virus; RNA sequencing; Suckling mouse; microRNA