title: Low generation polyamine dendrimers bearing flexible tetraethylene glycol as nanocarriers for plasmids and siRNA.
abstract: Low G1 generation polyamine dendrimers built around programmable, flexible, and short tetraethyleneglycol branches were readily prepared in a divergent manner using a combination of orthogonal AB3 or AB5 units and highly efficient chemical transformations based on Cu(i) catalyzed alkyne-azide cycloaddition (CUAAC) and thiol-ene click reactions. The constructs showed that the G1 polyamines with only twelve and eighteen amine surface groups can successfully deliver siRNA in human cells, with transfection efficiency comparable to that of Lipofectamine 2000®. Measurements of cell viability following transfection of plasmid DNA and siRNA showed that the dendritic polyamines are less cytotoxic than Lipofectamine 2000® and are thus preferable for biological applications.
authors: Pharmaqam, Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Succ. Centre-ville, Montréal, Canada H3C 3P8. roy.rene@uqam.ca.
authors: Rishi
authors: R
authors: Sharma
authors: Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir-William-Osler, Montreal, Quebec H3G 1Y6, Canada. dusica.maysinger@mcgill.ca.
authors: Issan
authors: I
authors: Zhang
authors: Pharmaqam, Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Succ. Centre-ville, Montréal, Canada H3C 3P8. roy.rene@uqam.ca.
authors: Tze Chieh
authors: TC
authors: Shiao
authors: Department of Innovative Technologies, University of Applied Sciences and Arts of Southern Switzerland, Galleria 2, 6928 Manno, Switzerland.
authors: Giovanni M
authors: GM
authors: Pavan
authors: Department of Pharmacology and Therapeutics, McGill University, 3655 Promenade Sir-William-Osler, Montreal, Quebec H3G 1Y6, Canada. dusica.maysinger@mcgill.ca.
authors: Dusica
authors: D
authors: Maysinger
authors: Pharmaqam, Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Succ. Centre-ville, Montréal, Canada H3C 3P8. roy.rene@uqam.ca.
authors: René
authors: R
authors: Roy
mesh_terms: D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D011073:Polyamines; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols