title: Aedes aegypti anti-salivary gland antibody concentration and dengue virus exposure history in healthy individuals living in an endemic area in Colombia.
abstract: BACKGROUND
  Mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. IgG antibodies against dengue virus (DENV-IgG) are indicators of previous exposure. The risk of DENV transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology.
  
  
  OBJECTIVE
  To determine the effect of the presence of Aedes aegypti mosquitos in different stages in households and the history of dengue exposure on vector-human contact determined by the level of anti-salivary protein antibodies in people living in a Colombian endemic area.
  
  
  METHODS
  A pilot study of 58 households and 55 human subjects was conducted in Norte de Santander, Colombia. A questionnaire for socioeconomic factors was administered and houses were examined for the presence of Ae. aegypti specimens in the aquatic stages. The level of DENV-IgG antibodies (DENV-IgG), in addition to IgG and IgM anti- Ae. aegypti salivary gland extract (SGE) antibodies (SGE-IgG, SGE-IgM) were evaluated by ELISA using blood collected in filter paper.
  
  
  RESULTS
  We found a significant higher level of SGE-IgG antibodies in subjects living in houses with Ae. aegypti in aquatic stages. We also found a higher concentration of SGE-IgG antibodies in people exposed to DENV, a positive correlation between IgM-SGE and IgG-DENV and a negative correlation with IgG-SGE.
  
  
  CONCLUSIONS
  Anti-salivary proteins antibodies are consistent with the presence of Ae. aegypti aquatic stages inside houses and DENV-IgG antibodies concentrations.
authors: Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.
authors: Berlín
authors: B
authors: Londoño-Rentería
authors: Hospital Local Los Patios, Norte de Santander, Colombia.
authors: Jenny C
authors: JC
authors: Cárdenas
authors: Pathobiological Sciences, Vector-borne Disease Laboratories, School of Veterinary Medicine, Louisiana State University.
authors: Jennifer E
authors: JE
authors: Giovanni
authors: Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.
authors: Lucio
authors: L
authors: Cárdenas
authors: Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.
authors: Paloma
authors: P
authors: Villamizar
authors: Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.
authors: Jennifer
authors: J
authors: Rolón
authors: Pathobiological Sciences, Vector-borne Disease Laboratories, School of Veterinary Medicine, Louisiana State University.
authors: Daniel M
authors: DM
authors: Chisenhall
authors: Pathobiological Sciences, Vector-borne Disease Laboratories, School of Veterinary Medicine, Louisiana State University.
authors: Rebecca C
authors: RC
authors: Christofferson
authors: Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.
authors: Daisy J
authors: DJ
authors: Carvajal
authors: Grupo de Investigaciones en Enfermedades Parasitarias e Infecciosas, Universidad de Pamplona, Norte de Santander, Colombia.
authors: Omar G
authors: OG
authors: Pérez
authors: School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA.
authors: Dawn M
authors: DM
authors: Wesson
authors: Department of Pathology, Microbiology and Immunology, University of South Carolina, Columbia, SC, USA.
authors: Christopher N
authors: CN
authors: Mores
mesh_terms: D000293:Adolescent; D000328:Adult; D000330:Aedes / Q000254:growth & development / Q000276:immunology* / Q000821:virology; D000368:Aged; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003105:Colombia / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology* / Q000635:transmission; D003716:Dengue Virus / Q000145:classification / Q000276:immunology*; D019353:Endemic Diseases; D004781:Environmental Exposure; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006798:Housing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D019476:Insect Proteins / Q000276:immunology*; D007303:Insect Vectors / Q000276:immunology* / Q000821:virology; D007814:Larva; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D012471:Salivary Proteins and Peptides / Q000276:immunology*; D012959:Socioeconomic Factors; D011795:Surveys and Questionnaires; D014881:Water Supply; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D019476:Insect Proteins; D012471:Salivary Proteins and Peptides