title: Genetic Manipulation of Nocardia Species.
abstract: Nocardia spp. are aerobic, Gram-positive, catalase positive, and non-motile actinomycetes. They are associated with human infections. However, some species produce important natural products, degrade toxic chemicals, and are involved in biotransformation of valuable products. The lack of robust genetic tools has hindered detailed studies and advanced research. This unit describes the major genetic engineering approaches using Nocardia sp. CS682 as a prototype. These methods will certainly help in understanding the basis of their pathogenicity as well as biosynthetic and biotransforming abilities. It can be expected that knowledge of the biochemistry behind their pathogenicity will be crucial in developing effective treatment strategies. These genetic tools can be utilized to develop rational metabolic engineering approaches for crafting host strains with higher production or biotransformation ability.
authors: Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
authors: Dipesh
authors: D
authors: Dhakal
authors: Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
authors: Amit
authors: A
authors: Kumar Jha
authors: Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
authors: Anaya
authors: A
authors: Pokhrel
authors: Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
authors: Anil
authors: A
authors: Shrestha
authors: Institute of Biomolecule Reconstruction, Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam, Republic of Korea.
authors: Jae Kyung
authors: JK
authors: Sohng
mesh_terms: D001431:Bacteriological Techniques; D005821:Genetic Techniques; D009615:Nocardia / Q000235:genetics*
keywords: Nocardia spp; RNA isolation; genomic DNA; protein extraction; transformation