title: Coupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays.
abstract: Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process. Fine mapping of epitope specific antibodies is difficult when they are elicited at relatively low levels. In our recent study focused at developing an HIV-1 vaccine, we immunized rabbits with hyperglycosylated stable core immunogens, which were designed using high resolution structural information to elicit antibodies against the primary receptor-binding, CD4-binding site on HIV-1 gp120. Using a solid phase adsorption assay, we could map the serum antibodies to the conserved CD4-binding site, a known broadly neutralizing determinant on exterior envelope glycoprotein, gp120.
authors: Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, USA.
authors: Jidnyasa
authors: J
authors: Ingale
authors: Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, USA; IAVI Neutralizing Antibody Center at TSRI, La Jolla, USA.
authors: Richard T
authors: RT
authors: Wyatt