title: Acute and chronic exposure to Tyrophagus putrescentiae induces allergic pulmonary response in a murine model.
abstract: BACKGROUND
  Tyrophagus putrescentiae (Tp) is a source of aeroallergen that causes allergic diseases.
  
  
  OBJECTIVE
  To describe an acute and chronic murine model of allergic asthma with Tp extract with no systemic sensitization and no use of adjuvant.
  
  
  METHODS
  Mites from dust sample were cultured and a raw extract was produced. Female BALB/c mice (6-8 weeks) were challenged intranasally with Tp extract or Dulbecco's phosphate-buffered saline, for 10 consecutive days (acute protocol) or for 6 weeks (chronic protocol). Twenty-four hours after the last intranasal challenge, bronchoalveolar lavage fluid (BALF) was performed for total and differential cells count, cytokine analysis, and eosinophil peroxidase activity. Lung tissue was also removed for histopathologic analysis.
  
  
  RESULTS
  Tp extract has shown a significant increase in total cells count from BALF as well as an increase in absolute eosinophils count, eosinophil peroxidase activity, interleukin (IL)-5 and IL-13 levels, in both acute and chronic protocols. Peribronchovascular infiltrate, goblet cells hyperplasia and collagen deposition were shown in the airways of acute and chronic Tp-exposed mice.
  
  
  CONCLUSIONS
  Our data suggest that the intranasal exposure to Tp extract, with no systemic sensitization and no use of adjuvants, induces a robust allergic inflammation in the lungs of mice, in both acute and chronic models. Our Tp extract seems to be a potent allergen extract which may be used in asthma model studies.
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Nailê Karine
authors: NK
authors: Nuñez
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Aline Andrea
authors: AA
authors: da Cunha
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Moisés
authors: M
authors: Dos Santos Dutra
authors: Laboratory of Parasitology, Universidade Regional Integrada do Alto Uruguai e das Missões, Santiago 97700-000, Brazil.
authors: Gustavo Leivas
authors: GL
authors: Barbosa
authors: Laboratory of Molecular Parasitology, Institute of Biomedical Research, PUCRS, Porto Alegre 90610-000, Brazil.
authors: Alessandra Loureiro
authors: AL
authors: Morassutti
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Rodrigo Godinho
authors: RG
authors: de Souza
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Mauro Henrique Moraes
authors: MH
authors: Vargas
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Géssica Luana
authors: GL
authors: Antunes
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Josiane Silva
authors: JS
authors: Silveira
authors: Laboratory of Acarology, Natural Science Museum, Centro Universitário UNIVATES, Lajeado 95900-000, Brazil.
authors: Guilherme Liberato
authors: GL
authors: da Silva
authors: Laboratory of Pediatric Respirology, Infant Center, Institute of Biomedical Research, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
authors: Paulo Márcio
authors: PM
authors: Pitrez
keywords: Allergy; Asthma; Mice; Storage Mites; Tyrophagus putrescentiae