title: Pathogenicity of Candida albicans isolates from bloodstream and mucosal candidiasis assessed in mice and Galleria mellonella.
abstract: The working hypothesis of this study was to elucidate a possible association between the pathogenic potential of Candida albicans strains with a clinical entity, systemic versus superficial candidiasis. Specifically, we assessed the pathogenicity of two groups of clinical C. albicans isolates: isolates from bloodstream infection (S) versus isolates from vaginitis patients (M), in two experimental in vivo systems - mice and Galleria melonella, in comparison to a control strain (CBS 562). Mice and G. mellonella larvae were inoculated with CBS 562 and the different S and M isolates, and followed up for survival rate and survival time during 30 and 7 days, respectively. Candida kidney colonization of mice was assessed by histopathology and colony-forming units' enumeration. The results revealed: (1) S and M isolates had different behavior patterns in the two models and varied in different parameters; (2) no statistically significant difference in pathogenicity between S and M isolates as whole groups was noted; (3) S14 was the most virulent isolate and close to the standard strain CBS 562 in both models. This study is distinctive in its outline combining two different groups of C. albicans clinical isolates originating from two different clinical entities that were assessed in vivo concurrently in two models.
authors: Department of clinical microbiology and immunology, Sackler school of medicine, Tel-Aviv university, 69978 Tel-Aviv, Israel.
authors: M
authors: M
authors: Frenkel
authors: Department of clinical microbiology and immunology, Sackler school of medicine, Tel-Aviv university, 69978 Tel-Aviv, Israel.
authors: M
authors: M
authors: Mandelblat
authors: Mycology department, Spanish national center for microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
authors: A
authors: A
authors: Alastruey-Izquierdo
authors: Institute of pathology, Assaf Harofeh medical center, Zerifin, Israel.
authors: S
authors: S
authors: Mendlovic
authors: Department of clinical microbiology and immunology, Sackler school of medicine, Tel-Aviv university, 69978 Tel-Aviv, Israel; City of Hope, Beckman research institute, department of immunology, Duarte, CA, USA.
authors: R
authors: R
authors: Semis
authors: Department of clinical microbiology and immunology, Sackler school of medicine, Tel-Aviv university, 69978 Tel-Aviv, Israel. Electronic address: segale@post.tau.ac.il.
authors: E
authors: E
authors: Segal
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D053208:Kaplan-Meier Estimate; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D007814:Larva / Q000382:microbiology; D051379:Mice; D008813:Mice, Inbred ICR; D009036:Moths / Q000382:microbiology*; D014774:Virulence
keywords: Candida albicans; Galleria mellonella model; Galleria mellonella modèle; Modèle souris; Mouse model