title: Anionic clay as the drug delivery vehicle: tumor targeting function of layered double hydroxide-methotrexate nanohybrid in C33A orthotopic cervical cancer model.
abstract: Methotrexate (MTX), an anticancer agent, was successfully intercalated into the anionic clay, layered double hydroxides to form a new nanohybrid drug. The coprecipitation and subsequent hydrothermal method were used to prepare chemically, structurally, and morphologically well-defined two-dimensional drug-clay nanohybrid. The resulting two-dimensional drug-clay nanohybrid showed excellent colloidal stability not only in deionized water but also in an electrolyte solution of Dulbecco's Modified Eagle's Medium with 10% fetal bovine serum, in which the average particle size in colloid and the polydispersity index were determined to be around 100 and 0.250 nm, respectively. The targeting property of the nanohybrid drug was confirmed by evaluating the tumor-to-blood and tumor-to-liver ratios of the MTX with anionic clay carrier, and these ratios were compared to those of free MTX in the C33A orthotopic cervical cancer model. The biodistribution studies indicated that the mice treated with the former showed 3.5-fold higher tumor-to-liver ratio and fivefold higher tumor-to-blood ratio of MTX than those treated with the latter at 30 minutes postinjection.
authors: Center for Intelligent Nano-Bio Materials, Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Korea.
authors: Goeun
authors: G
authors: Choi
authors: Center for Intelligent Nano-Bio Materials, Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Korea.
authors: Huiyan
authors: H
authors: Piao
authors: Advanced Materials Research Chair, Chemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors: Zeid A
authors: ZA
authors: Alothman
authors: Future Industries Institute, University of South Australia, Mawson Lakes, SA, Australia.
authors: Ajayan
authors: A
authors: Vinu
authors: Department of Bioengineering, College of Engineering, Hanyang University, Seoul, Korea.
authors: Chae-Ok
authors: CO
authors: Yun
authors: Center for Intelligent Nano-Bio Materials, Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Korea.
authors: Jin-Ho
authors: JH
authors: Choy
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D000838:Anions; D000964:Antimetabolites, Antineoplastic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000077215:Clay; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D006878:Hydroxides / Q000737:chemistry*; D008727:Methotrexate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000538:Aluminum Silicates; D000838:Anions; D000964:Antimetabolites, Antineoplastic; D006878:Hydroxides; C031356:hydroxide ion; D000077215:Clay; D008727:Methotrexate
keywords: anionic clay; biodistribution; cervical cancer; colloidal stability; layered double hydroxide; methotrexate