title: Disease duration and age influence CARD15 expression in Crohn's disease.
abstract: One of the susceptibility genes in Crohn's disease (CD) is CARD15. Our study examined the relationship between peripheral CARD15 expression and phenotype and duration of CD, treatment methods and inflammatory indices. Sixty patients with CD and 30 healthy volunteers as controls were enrolled in the study. Total RNA was isolated from peripheral blood mononuclear cells (PBMCs) with E.Z.N.A. Total RNA Kit (Omega Bio-tek) then quantitative real-time PCR was performed on the ABI Prism 7900 HT Real-Time PCR System. CARD15 gene expression in PBMCs in CD was significantly higher than in the control group. The highest level of gene expression was found in CD patients in the fourth decade of life. The mRNA level of the CARD15 gene was higher in patients with disease duration between 12 and 60 months. A positive correlation was found between erythrocyte sedimentation rate (ESR) and gene expression level. Gene expression increased with increasing level of C-reactive protein and ESR, but it was not statistically significant. CARD15 expression significantly decreased in CD patients treated with anti-TNFα agents compared to azathioprine or steroid treatment groups. Expression of the CARD15 gene in Crohn›s disease is higher than in healthy individuals. Disease duration and age of patients seem to be the most important factors influencing CARD15 expression.
authors: Department of Gastroenterology and Hepatology, Wroclaw Medical University, Poland, 50-556 Wroclaw, Borowska 213; Department of Gastroenterology and Hepatology. Dietetics Unit, Wroclaw Medical University.
authors: Elżbieta
authors: E
authors: Poniewierka
authors: Department of Gastroenterology and Hepatology, Wroclaw Medical University, Poland, 50-556 Wroclaw, Borowska 213.
authors: Katarzyna
authors: K
authors: Neubauer
authors: Department of Gastroenterology and Hepatology, Wroclaw Medical University, Poland, 50-556 Wroclaw, Borowska 213.
authors: Radosław
authors: R
authors: Kempiński
authors: Department of Forensic Science, Wroclaw Medical University, Poland.
authors: Anna
authors: A
authors: Sadakierska-Chudy
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D003424:Crohn Disease / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D053473:Nod2 Signaling Adaptor Protein / Q000096:biosynthesis / Q000235:genetics*; D010641:Phenotype; D014176:Protein Biosynthesis; D013997:Time Factors; D055815:Young Adult
chemical_list: C429018:NOD2 protein, human; D053473:Nod2 Signaling Adaptor Protein