title: The effect of oxalic and itaconic acids on threo-Ds-isocitric acid production from rapeseed oil by Yarrowia lipolytica.
abstract: The effect of oxalic and itaconic acids, the inhibitors of the isocitrate lyase, on the production of isocitric acid by the wild strain Yarrowia lipolytica VKM Y-2373 grown in the medium containing rapeseed oil was studied. In the presence of oxalic and itaconic acids, strain Y. lipolytica accumulated in the medium isocitric acid (70.0 and 82.7 g/L, respectively) and citric acid (23.0 and 18.4 g/L, respectively). In control experiment, when the inhibitors were not added to the medium, the strain accumulated isocitric and citric acids at concentrations of 62.0 and 28.0 g/L, respectively. Thus, the use of the oxalic and itaconic acids as additives to the medium is a simple and convenient method of isocitric acid production with a minimum content of citric acid.
authors: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia. Electronic address: kamzolova@rambler.ru.
authors: Svetlana V
authors: SV
authors: Kamzolova
authors: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors: Ramil K
authors: RK
authors: Allayarov
authors: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors: Julia N
authors: JN
authors: Lunina
authors: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino 142290, Russia.
authors: Igor G
authors: IG
authors: Morgunov
mesh_terms: D019343:Citric Acid / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated; D007218:Industrial Microbiology / Q000379:methods; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism; D007523:Isocitrates / Q000378:metabolism*; D019815:Oxalic Acid / Q000378:metabolism / Q000494:pharmacology*; D010938:Plant Oils / Q000378:metabolism*; D000074262:Rapeseed Oil; D013386:Succinates / Q000378:metabolism / Q000494:pharmacology*; D025062:Yarrowia / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D005229:Fatty Acids, Monounsaturated; D007523:Isocitrates; D010938:Plant Oils; D000074262:Rapeseed Oil; D013386:Succinates; D019343:Citric Acid; D019815:Oxalic Acid; C034219:isocitric acid; D007522:Isocitrate Lyase; C005229:itaconic acid
keywords: Isocitrate lyase; Itaconic acid; Oxalic acid; Threo-Ds-isocitric acid; Yarrowia lipolytica yeast