title: In vitro cytotoxic screening of 31 crude extracts of Thai herbs on a chondrosarcoma cell line and primary chondrocytes and apoptotic effects of selected extracts.
abstract: Thirty-one dichloromethane and methanol crude extracts of 16 herb species used in Thai traditional folk medicine were studied for their cytotoxic activities on the SW 1353 chondrosarcoma cell line and primary chondrocytes. Methyl thiazolyl tetrazolium (MTT) cell viability assay and flow cytometric method were used as screening tools for cytotoxicity testing. The half maximal inhibitory concentration (IC50) was measured and reported for each crude extract. Apoptosis, necrosis, and cell viability were measured by flow cytometry at IC50. Two out of 31 herbal extracts, methanol extracts of Paris polyphylla var. chinensis and Ficus thailandica C.C. Berg & S. Gardner, showed potent anticancer activity. They demonstrated high apoptosis induction activity in SW 1353 cells but had less effect on percentage of viability and necrosis of normal chondrocyte cells. Cytotoxic screening and apoptosis assays suggest the potential anticancer activity of some plants used in Thai traditional medicine and provide information concerning their direct effects.
authors: Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors: Napat
authors: N
authors: Ruamrungsri
authors: Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors: Puntita
authors: P
authors: Siengdee
authors: Department of Animal and Aquatic Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors: Korawan
authors: K
authors: Sringarm
authors: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors: Siriwadee
authors: S
authors: Chomdej
authors: Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry and Center of Excellence for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors: Siriwan
authors: S
authors: Ongchai
authors: Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. korakot.n@cmu.ac.th.|Excellence Center in Osteology Research and Training Center, Chiang Mai University, Chiang Mai, 50200, Thailand. korakot.n@cmu.ac.th.
authors: Korakot
authors: K
authors: Nganvongpanit
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002452:Cell Count; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects; D002813:Chondrosarcoma / Q000473:pathology*; D004285:Dogs; D006801:Humans; D020128:Inhibitory Concentration 50; D008752:Methylene Chloride; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D008752:Methylene Chloride
keywords: Apoptosis; Chondrocyte; Chondrosarcoma; Cytotoxic screening; Medicinal plants