title: Determination of marbofloxacin in plasma and synovial fluid by ultrafiltration followed by HPLC-MS/MS.
abstract: A rapid LC-MS/MS method for the determination of marbofloxacin in plasma and synovial fluid is presented in this study. The method uses a rapid sample preparation which only requires an ultrafiltration step with centrifugal filter devices. The optimized procedure allows a minimal need of sample (175 μL), particularly useful for synovial fluid samples which amount is rather limited; it is simple, rapid and easily applicable providing anyhow a satisfactory clean up, demonstrated by post-infusion experiments. On the other hand to maximize the speed of the analysis an ultrafast chromatographic separation has been obtained by selecting a column of 20 mm; the reduced run-time is suitable for processing numerous samples on a daily basis. Linearity was assessed in the range 5-2500 ng mL(-1); ofloxacin was used as internal standard. LOD and LOQ were respectively 1 and 5 ng/mL. The method was successfully applied to a set of samples generated during an experimental veterinary study.
authors: Sapienza University of Rome, Department of Chemistry, 00185 Rome, Italy.
authors: Camilla
authors: C
authors: Montesano
authors: Sapienza University of Rome, Department of Chemistry, 00185 Rome, Italy.
authors: Roberta
authors: R
authors: Curini
authors: University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment, 64023 Mosciano Sant'Angelo, TE, Italy. Electronic address: msergi@unite.it.
authors: Manuel
authors: M
authors: Sergi
authors: University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment, 64023 Mosciano Sant'Angelo, TE, Italy.
authors: Dario
authors: D
authors: Compagnone
authors: Veterinary Teaching Hospital, University of Teramo, 64100 Teramo, Italy.
authors: Gianluca
authors: G
authors: Celani
authors: Veterinary Teaching Hospital, University of Teramo, 64100 Teramo, Italy.
authors: Vincenzo
authors: V
authors: Varasano
authors: Veterinary Teaching Hospital, University of Teramo, 64100 Teramo, Italy.
authors: Lucio
authors: L
authors: Petrizzi
authors: University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment, 64023 Mosciano Sant'Angelo, TE, Italy.
authors: Michele
authors: M
authors: Amorena
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones / Q000097:blood* / Q000737:chemistry*; D015242:Ofloxacin / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D013582:Synovial Fluid / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014462:Ultrafiltration / Q000379:methods
chemical_list: D024841:Fluoroquinolones; C080260:marbofloxacin; D015242:Ofloxacin
keywords: Bovine; LC–MS/MS; Marbofloxacin; Plasma; Synovial fluid; Ultrafiltration