title: Allogeneic Transplantation of Periodontal Ligament-Derived Multipotent Mesenchymal Stromal Cell Sheets in Canine Critical-Size Supra-Alveolar Periodontal Defect Model.
abstract: Periodontitis is a chronic inflammatory disease that induces the destruction of tooth-supporting tissues, followed by tooth loss. Although several approaches have been applied to periodontal regeneration, complete periodontal regeneration has not been accomplished. Tissue engineering using a combination of cells and scaffolds is considered to be a viable alternative strategy. We have shown that autologous transplantation of periodontal ligament-derived multipotent mesenchymal stromal cell (PDL-MSC) sheets regenerates periodontal tissue in canine models. However, the indications for autologous cell transplantation in clinical situations are limited. Therefore, this study evaluated the safety and efficacy of allogeneic transplantation of PDL-MSC sheets using a canine horizontal periodontal defect model. Canine PDL-MSCs were labeled with enhanced green fluorescent protein (EGFP) and were cultured on temperature-responsive dishes. Three-layered cell sheets were transplanted around denuded root surfaces either autologously or allogeneically. A mixture of β-tricalcium phosphate and collagen gel was placed on the bone defects. Eight weeks after transplantation, dogs were euthanized and subjected to microcomputed tomography and histological analyses. RNA and DNA were extracted from the paraffin sections to verify the presence of EGFP at the transplantation site. Inflammatory markers from peripheral blood sera were quantified using an enzyme-linked immunosorbent assay. Periodontal regeneration was observed in both the autologous and the allogeneic transplantation groups. The allogeneic transplantation group showed particularly significant regeneration of newly formed cementum, which is critical for the periodontal regeneration. Serum levels of inflammatory markers from peripheral blood sera showed little difference between the autologous and allogeneic groups. EGFP amplicons were detectable in the paraffin sections of the allogeneic group. These results suggest that allogeneic PDL-MSC sheets promoted periodontal tissue regeneration without side effects. Therefore, allogeneic transplantation of PDL-MSC sheets has a potential to become an alternative strategy for periodontal regeneration.
authors: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
authors: Yuka
authors: Y
authors: Tsumanuma
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University , Tokyo, Japan .
authors: Takanori
authors: T
authors: Iwata
authors: Department of Educational Media Development, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
authors: Atsuhiro
authors: A
authors: Kinoshita
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University , Tokyo, Japan .
authors: Kaoru
authors: K
authors: Washio
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University , Tokyo, Japan .
authors: Toshiyuki
authors: T
authors: Yoshida
authors: Department of Behavioral Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
authors: Azusa
authors: A
authors: Yamada
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University , Tokyo, Japan .
authors: Ryo
authors: R
authors: Takagi
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University , Tokyo, Japan .
authors: Masayuki
authors: M
authors: Yamato
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University , Tokyo, Japan .
authors: Teruo
authors: T
authors: Okano
authors: Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , Tokyo, Japan .
authors: Yuichi
authors: Y
authors: Izumi
keywords: regeneration; stem cells; tissue engineering