title: Rotational imaging optical coherence tomography for full-body mouse embryonic imaging.
abstract: Optical coherence tomography (OCT) has been widely used to study mammalian embryonic development with the advantages of high spatial and temporal resolutions and without the need for any contrast enhancement probes. However, the limited imaging depth of traditional OCT might prohibit visualization of the full embryonic body. To overcome this limitation, we have developed a new methodology to enhance the imaging range of OCT in embryonic day (E) 9.5 and 10.5 mouse embryos using rotational imaging. Rotational imaging OCT (RI-OCT) enables full-body imaging of mouse embryos by performing multiangle imaging. A series of postprocessing procedures was performed on each cross-section image, resulting in the final composited image. The results demonstrate that RI-OCT is able to improve the visualization of internal mouse embryo structures as compared to conventional OCT.
authors: University of Houston, Department of Biomedical Engineering, Houston, 3605 Cullen Boulevard, Texas 77204, United States.
authors: Chen
authors: C
authors: Wu
authors: University of Houston, Department of Biomedical Engineering, Houston, 3605 Cullen Boulevard, Texas 77204, United States.
authors: Narendran
authors: N
authors: Sudheendran
authors: University of Houston, Department of Biomedical Engineering, Houston, 3605 Cullen Boulevard, Texas 77204, United States.
authors: Manmohan
authors: M
authors: Singh
authors: Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77584, United States.
authors: Irina V
authors: IV
authors: Larina
authors: Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77584, United States.
authors: Mary E
authors: ME
authors: Dickinson
authors: University of Houston, Department of Biomedical Engineering, Houston, 3605 Cullen Boulevard, Texas 77204, United StatesbBaylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77584, United StatescTo.
authors: Kirill V
authors: KV
authors: Larin
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D012399:Rotation; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*; D051598:Whole Body Imaging / Q000295:instrumentation / Q000379:methods*