title: Association of human papillomavirus and Chlamydia trachomatis with intraepithelial alterations in cervix samples.
abstract: The influence of different infectious agents and their association with human papillomavirus (HPV) in cervical carcinogenesis have not been completely elucidated. This study describes the association between cytological changes in cervical epithelium and the detection of the most relevant aetiological agents of sexually transmitted diseases. Samples collected from 169 patients were evaluated by conventional cytology followed by molecular analysis to detect HPV DNA, Chlamydia trachomatis, herpes simplex virus 1 and 2,Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, andTreponema pallidum, besides genotyping for most common high-risk HPV. An association between cytological lesions and different behavioural habits such as smoking and sedentariness was observed. Intraepithelial lesions were also associated with HPV and C. trachomatis detection. An association was also found between both simple and multiple genotype infection and cytological changes. The investigation of HPV and C. trachomatisproved its importance and may be considered in the future for including in screening programs, since these factors are linked to the early diagnosis of patients with precursor lesions of cervical cancer.
authors: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors: Denise
authors: D
authors: Wohlmeister
authors: Departamento de Análises, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors: Débora Renz Barreto
authors: DR
authors: Vianna
authors: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors: Virgínia Etges
authors: VE
authors: Helfer
authors: Laboratório de Citologia Clínica, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors: Fabrícia
authors: F
authors: Gimenes
authors: Laboratório de Citologia Clínica, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors: Marcia Edilaine Lopes
authors: ME
authors: Consolaro
authors: Centro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.
authors: Regina Bones
authors: RB
authors: Barcellos
authors: Centro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.
authors: Maria Lucia
authors: ML
authors: Rossetti
authors: Departamento de Análises, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors: Luciane Noal
authors: LN
authors: Calil
authors: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors: Andréia
authors: A
authors: Buffon
authors: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors: Diogo André
authors: DA
authors: Pilger
mesh_terms: D000293:Adolescent; D000328:Adult; D063646:Carcinogenesis; D002584:Cervix Uteri / Q000382:microbiology* / Q000473:pathology; D002690:Chlamydia Infections / Q000150:complications / Q000453:epidemiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D060085:Coinfection; D003430:Cross-Sectional Studies; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000302:isolation & purification*; D055088:Early Detection of Cancer / Q000379:methods; D004848:Epithelium / Q000821:virology; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D018259:Herpesvirus 1, Human / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D058889:Molecular Typing; D045704:Mycoplasma genitalium / Q000302:isolation & purification; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology; D012307:Risk Factors; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000382:microbiology*; D014210:Treponema pallidum / Q000302:isolation & purification; D014246:Trichomonas vaginalis / Q000302:isolation & purification; D002583:Uterine Cervical Neoplasms / Q000382:microbiology / Q000517:prevention & control*; D055815:Young Adult
chemical_list: D004279:DNA, Viral