title: Optimization of cellulase-assisted extraction process and antioxidant activities of polysaccharides from Tricholoma mongolicum Imai.
abstract: BACKGROUND
  Tricholoma mongolicum Imai is a well-known edible and medicinal mushroom which in recent years has attracted increasing attention because of its bioactivities. In this study, water-soluble polysaccharides were extracted from T. mongolicum Imai by cellulase-assisted extraction and their antioxidant activities were investigated.
  
  
  RESULTS
  In order to improve the yield of polysaccharides, four variables, cellulase amount (X1 ), pH (X2 ), temperature (X3 ) and extraction time (X4 ), were investigated with a Box-Behnken design. The optimal conditions were predicted to be cellulase amount of 20 g kg(-1) , pH of 4.0, temperature of 50 °C and extraction time of 127 min, with a predicted polysaccharide yield of 190.1 g kg(-1) . The actual yield of polysaccharides under these conditions was 189.6 g kg(-1) , which matched the predicted value well. The crude polysaccharides were purified to obtain four fractions, and characterization of each was carried out. In addition, antioxidant properties of four polysaccharides assessed by 1,1-diphenyl-2-picryldydrazyl (DPPH) and hydroxyl radical-scavenging assays indicated that polysaccharides from T. mongolicum Imai (TMIPs) possessed antioxidant activity in a dose-dependent manner.
  
  
  CONCLUSIONS
  TMIPs show moderate antioxidant activities in vitro. Therefore it is suggested that TMIPs are potential natural antioxidants for use in functional foods. © 2016 Society of Chemical Industry.
authors: Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China.
authors: Yong-Ming
authors: YM
authors: Zhao
authors: College of Agriculture and Forestry Science and Technology, Hebei North University, Zhangjiakou, 075000, China.
authors: Jin-Hui
authors: JH
authors: Song
authors: Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China.|Hebei Medical University, Shijiazhuang, 050017, China.
authors: Jin
authors: J
authors: Wang
authors: Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China.
authors: Jian-Ming
authors: JM
authors: Yang
authors: Department of Pharmacy, Hebei North University, Zhangjiakou, 075000, China.
authors: Zhi-Bao
authors: ZB
authors: Wang
authors: College of Agriculture and Forestry Science and Technology, Hebei North University, Zhangjiakou, 075000, China.
authors: Ying-Hui
authors: YH
authors: Liu
mesh_terms: D000465:Algorithms; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002240:Carbohydrate Sequence; D002480:Cellulase / Q000378:metabolism*; D002681:China; D045424:Complex Mixtures / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D005503:Food Additives / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D057141:Food, Preserved / Q000032:analysis / Q000191:economics; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D048690:Fruiting Bodies, Fungal / Q000737:chemistry* / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D008970:Molecular Weight; D013223:Statistics as Topic; D013997:Time Factors; D055439:Tricholoma / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D045424:Complex Mixtures; D005503:Food Additives; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D002480:Cellulase
keywords: antioxidant; cellulase-assisted extraction; polysaccharides; response surface methodology