title: Comparative effects of two different doses of low-level laser therapy on wound healing third-degree burns in rats.
abstract: Burns are injuries caused by direct or indirect contact to chemical, physical, or biological agents. Low-level laser therapy (LLLT) is a promising treatment since it is low-cost, non-invasive, and induces cell proliferation. This study aimed to investigate the effects of LLLT (660 nm) at two different fluences (12.5 J/cm(2) and 25 J/cm(2) ) per point of application on third-degree burns in rats. Thirty rats (Wistar) divided into GC, GL12.5, and GL25 were used in the study, and submitted to burn injury through a soldering iron at 150°C, pressed on their back for 10 s. LLLT was applied immediately, and 2, 4, 6, and 8 days after wound induction. Histological analysis revealed a decreased inflammatory infiltrate in the group treated with 25 J/cm(2) , and intense inflammatory infiltrate in the control group and in the group treated with 12.5 J/cm(2) . The immunostaining of COX-2 was more intense in the control groups and in the group treated with 12.5 J/cm(2) than in the group treated with 25 J/cm(2) . Conversely, VEGF immunomarking was more expressive in the group treated with 25 J/cm(2) than it was in the other two groups. Therefore, our findings suggest that the use of 25 J/cm(2) and 1 J of energy was more effective in stimulating the cellular processes involved in tissue repair on third-degree burns in rats by reducing the inflammatory phase, and stimulating angiogenesis, thus restoring the local microcirculation which is essential for cell migration.
authors: Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
authors: Patricia
authors: P
authors: Brassolatti
authors: Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
authors: Paulo Sérgio
authors: PS
authors: Bossini
authors: Department of Physiotherapy, Graduate of Physiotherapy, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
authors: Maria Carolina Derêncio
authors: MC
authors: Oliveira
authors: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil.
authors: Hueliton Wilian
authors: HW
authors: Kido
authors: Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil.
authors: Carla Roberta
authors: CR
authors: Tim
authors: DMC Equipment Import and Export- Co. Ltda, São Carlos, SP, Brazil.
authors: Luciana
authors: L
authors: Almeida-Lopes
authors: Department of Medicine, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
authors: Lucimar Retto Da Silva
authors: LR
authors: De Avó
authors: Department of Physics, Post-Graduate Program of Biotechnology Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
authors: Fernando M
authors: FM
authors: Araújo-Moreira
authors: Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
authors: Nivaldo Antonio
authors: NA
authors: Parizotto
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D004195:Disease Models, Animal; D006801:Humans; D028022:Low-Level Light Therapy / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000528:radiation effects
keywords: burns; low-level laser therapy; wound healing