title: Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics.
abstract: Tissues are heterogeneous in their components. If cells of interest are a minor population of collected tissue, it would be difficult to obtain genetic or genomic information of the interested cell population with conventional genomic DNA extraction from the collected tissue. Single-cell DNA analysis is important in the analysis of genetics of cell clonality, genetic anticipation, and single-cell DNA polymorphisms. Single-cell PCR using Single Cell Ampligrid/GeXP platform is described in this chapter.
authors: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, 8515 Fannin Street, Houston, TX, 77054, USA. cok@mdanderson.org.
authors: Chi Young
authors: CY
authors: Ok
authors: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, 8515 Fannin Street, Houston, TX, 77054, USA. rsingh@mdanderson.org.
authors: Rajesh R
authors: RR
authors: Singh
authors: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, 8515 Fannin Street, Houston, TX, 77054, USA. AAAlmohammedsali@mdanderson.org.
authors: Alaa A
authors: AA
authors: Salim
mesh_terms: D006801:Humans; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D059010:Single-Cell Analysis / Q000379:methods*
keywords: Cancer; Diagnostics; Quantitative; RT-PCR; Single-cell analysis