title: Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.
abstract: We developed a scanning hard x-ray microscope using a new class of x-ray nano-focusing optic called a multilayer Laue lens and imaged a chromosome with nanoscale spatial resolution. The combination of the hard x-ray's superior penetration power, high sensitivity to elemental composition, high spatial-resolution and quantitative analysis creates a unique tool with capabilities that other microscopy techniques cannot provide. Using this microscope, we simultaneously obtained absorption-, phase-, and fluorescence-contrast images of Pt-stained human chromosome samples. The high spatial-resolution of the microscope and its multi-modality imaging capabilities enabled us to observe the internal ultra-structures of a thick chromosome without sectioning it.
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Hanfei
authors: H
authors: Yan
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Evgeny
authors: E
authors: Nazaretski
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Kenneth
authors: K
authors: Lauer
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Xiaojing
authors: X
authors: Huang
authors: Diamond Light Source Ltd, Didcot, Oxfordshire, OX11 0DE, UK.
authors: Ulrich
authors: U
authors: Wagner
authors: Diamond Light Source Ltd, Didcot, Oxfordshire, OX11 0DE, UK.
authors: Christoph
authors: C
authors: Rau
authors: London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK.|Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, OX11 0FA, UK.
authors: Mohammed
authors: M
authors: Yusuf
authors: London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK.|Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, OX11 0FA, UK.
authors: Ian
authors: I
authors: Robinson
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Sebastian
authors: S
authors: Kalbfleisch
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Li
authors: L
authors: Li
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Nathalie
authors: N
authors: Bouet
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Juan
authors: J
authors: Zhou
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.|Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois 60439, USA.
authors: Ray
authors: R
authors: Conley
authors: National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors: Yong S
authors: YS
authors: Chu
mesh_terms: D002460:Cell Line; D002875:Chromosomes / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D064847:Multimodal Imaging / Q000379:methods*; D036103:Nanotechnology; D010984:Platinum / Q000737:chemistry; D013194:Staining and Labeling
chemical_list: D010984:Platinum