title: Facile endothelial cell micropatterning induced by reactive oxygen species on polydimethylsiloxane substrates.
abstract: Sophisticated cell pattern provides unique cellular assay platform for studying cell to cell interaction, cellular differentiation and signaling, high-throughput cell response to chemicals. In this study, we demonstrated reactive oxygen species (ROS) mediated endothelial cell micropatterning on a polydimethylsiloxane (PDMS) substrate. The exposure of UV/O radiation led to the formation of ROS on the surface of PDMS, which could selectively prevent adhesion of endothelial cells. The degree of ROS amount was monitored according to the UV/O irradiation time, and at least 36 μM of ROS resulted in the precise cellular micropattern on the PDMS. The presence of ROS affected not only cellular detachment from the substrate, but also endothelial cell morphology such as cell spreading area, confluence, nuclear area and nuclear inverse aspect ratio. In addition, we could observe that the actin cytoskeleton of cells was also constricted due to ROS, thereby minimizing the focal adhesion area of vinculin. Compared with previously reported methods which use chemical treatment or nano/microstructure on the substrate, the proposed methodology is quite simple, accurate, and harmless to the patterned endothelial cells.
authors: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, South Korea.
authors: Jong Seob
authors: JS
authors: Choi
authors: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, South Korea.
authors: Do Hyun
authors: DH
authors: Kim
authors: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, South Korea. Electronic address: seots@kaist.ac.kr.
authors: Tae Seok
authors: TS
authors: Seo
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D048430:Cell Shape / Q000187:drug effects / Q000528:radiation effects; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D055616:Microtechnology / Q000379:methods*; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013997:Time Factors; D014466:Ultraviolet Rays; D016596:Vinculin / Q000378:metabolism
chemical_list: D004129:Dimethylpolysiloxanes; D017382:Reactive Oxygen Species; D016596:Vinculin; C013830:baysilon
keywords: Cell adhesion; Cell micropatterning; Cell morphology; Endothelial cell; Reactive oxygen species (ROS); Ultraviolet irradiation