title: Comparison of Incubation Solutions Prior to the Purification of Porcine Islet Cells.
abstract: For pancreatic islet transplantation, one of the most important steps of islet isolation is islet purification. The most common method of islet purification is density gradient centrifugation because there are differences in density between islets and acinar tissue. However, the density of islets/acinar tissue depends on several conditions, such as the incubation time before purification and the osmolality of the preincubation solution. In this study, we evaluated the impact of using two different preincubation solutions before purification. We used the University of Wisconsin (UW) solution and a new preservation solution (HN-1), which we recently developed. There were no significant differences between the two solutions in terms of the islet yield, rate of viability, and purity or stimulation index after purification. There were also no differences in the attainability and suitability of posttransplantation normoglycemia. Our study shows that the HN-1 solution is equivalent to the UW solution for preincubation before islet purification.
authors: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Takashi
authors: T
authors: Kawai
authors: † Department of Surgery, Chiba-East National Hospital, National Hospital Organization , Chiba , Japan.
authors: Hirofumi
authors: H
authors: Noguchi
authors: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Takashi
authors: T
authors: Kuise
authors: ‡ Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Atsuko
authors: A
authors: Nakatsuka
authors: ‡ Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Akihiro
authors: A
authors: Katayama
authors: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Noriko
authors: N
authors: Imagawa
authors: § Department of Primary Care and Medical Education, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Hitomi Usui
authors: HU
authors: Kataoka
authors: ¶ Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Niigata , Japan.
authors: Issei
authors: I
authors: Saitoh
authors: # Department of Socio-environmental Design, Hiroshima International University , Hiroshima , Japan.
authors: Yasufumi
authors: Y
authors: Noguchi
authors: * Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Masami
authors: M
authors: Watanabe
authors: Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors: Toshiyoshi
authors: T
authors: Fujiwara
keywords: HN-1 solution; Islet isolation; Islet transplantation; Preincubation; University of Wisconsin (UW) solution