title: Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
abstract: Plant terpenoids are high-value compounds broadly applied as food additives or fragrances in perfumes and cosmetics. Their biotechnological production in yeast offers an attractive alternative to extraction from plants. Here, we provide two optimized protocols for the production of the plant terpenoid trans-nootkatol with recombinant S. cerevisiae by either (I) converting externally added (+)-valencene with resting cells or (II) cultivating engineered self-sufficient production strains. By synthesis of the hydrophobic compounds in self-sufficient production cells, phase transfer issues can be avoided and the highly volatile products can be enriched in and easily purified from n-dodecane, which is added to the cell broth as second phase.
authors: ACIB - Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria. anita.emmerstorfer@acib.at.
authors: Anita
authors: A
authors: Emmerstorfer-Augustin
authors: ACIB - Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria.|Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology, Petersgasse 14/2, 8010, Graz, Austria.
authors: Harald
authors: H
authors: Pichler
mesh_terms: D001711:Biotransformation; D060847:Metabolic Engineering; D010944:Plants / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000378:metabolism
chemical_list: D013729:Terpenes; D055549:Volatile Organic Compounds
keywords: (+)-Valencene synthase; Biotransformation; Cytochrome P450 enzymes; Metabolic engineering; Plant terpenoids; S. cerevisiae; Yeast; tHMG1