title: Detection of C-reactive protein using nanoparticle-enhanced surface plasmon resonance using an aptamer-antibody sandwich assay.
abstract: High affinity DNA aptamers against C-reactive protein (CRP) were obtained using a microfluidic chip. The aptamers were then used for the construction of an Au nanoparticle enhanced surface plasmon resonance biosensor, which was introduced for the detection of CRP at concentrations ranging from 10 pM to 100 nM in diluted human serum.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha, 410082, China. yangxiaohai@hnu.edu.cn kmwang@hnu.edu.cn.
authors: Bin
authors: B
authors: Wu
authors: Rui
authors: R
authors: Jiang
authors: Qing
authors: Q
authors: Wang
authors: Jin
authors: J
authors: Huang
authors: Xiaohai
authors: X
authors: Yang
authors: Kemin
authors: K
authors: Wang
authors: Wenshan
authors: W
authors: Li
authors: Nandi
authors: N
authors: Chen
authors: Qing
authors: Q
authors: Li
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002097:C-Reactive Protein / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000906:Antibodies; D052157:Aptamers, Nucleotide; D006046:Gold; D002097:C-Reactive Protein