title: Efficacy and safety of electrochemotherapy combined with peritumoral IL-12 gene electrotransfer of canine mast cell tumours.
abstract: Electrochemotherapy combined with peritumoral interleukin-12 (IL-12) gene electrotransfer was used for treatment of mast cell tumours in 18 client-owned dogs. Local tumour control, recurrence rate, as well as safety of combined therapy were evaluated. One month after the therapy, no side effects were recorded and good local tumour control was observed with high complete responses rate which even increased during the observation period to 72%. IL-12 gene electrotransfer resulted in 78% of patients with detectable serum IFN-γ and/or IL-12 levels. In the treated tumours vascular changes as well as minimal T-lymphocytes infiltration was observed. After 1 week, the plasmid DNA was not detected intra- or peritumorally and no horizontal gene transfer was observed. In summary, our study demonstrates high antitumour efficacy of electrochemotherapy combined with IL-12 electrotransfer, which also prevented recurrences or distant metastases, as well as its safety and feasibility in treatment of canine mast cell tumours.
authors: Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.|Faculty of Health Sciences, University of Primorska, Isola, Slovenia.
authors: M
authors: M
authors: Cemazar
authors: Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors: J
authors: J
authors: Ambrozic Avgustin
authors: Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors: D
authors: D
authors: Pavlin
authors: Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
authors: G
authors: G
authors: Sersa
authors: Department of Veterinary Science, University of Pisa, Pisa, Italy.
authors: A
authors: A
authors: Poli
authors: Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors: A
authors: A
authors: Krhac Levacic
authors: Faculty of Health Sciences, University of Primorska, Isola, Slovenia.
authors: N
authors: N
authors: Tesic
authors: Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
authors: U
authors: U
authors: Lampreht Tratar
authors: Faculty of Health Sciences, University of Primorska, Isola, Slovenia.
authors: M
authors: M
authors: Rak
authors: Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors: N
authors: N
authors: Tozon
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy / Q000662:veterinary; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D053672:Electrochemotherapy / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D005260:Female; D018014:Gene Transfer Techniques / Q000009:adverse effects / Q000662:veterinary*; D018664:Interleukin-12 / Q000235:genetics*; D008297:Male; D034701:Mastocytosis, Cutaneous / Q000188:drug therapy / Q000662:veterinary*
chemical_list: D018664:Interleukin-12
keywords: dogs; efficacy; electrochemotherapy; interleukin-12; mast cell tumour; safety