title: Low pressure steam expansion pretreatment as a competitive approach to improve diosgenin yield and the production of fermentable sugar from Dioscorea zingiberensis C.H. Wright.
abstract: Development of efficient pretreatment methods which can disrupt the peripheral lignocellulose and even the parenchyma cells is of great importance for production of diosgenin from turmeric rhizomes. It was found that low pressure steam expansion pretreatment (LSEP) could improve the diosgenin yield by more than 40% compared with the case without pretreatment, while simultaneously increasing the production of fermentable sugar by 27.37%. Furthermore, little inhibitory compounds were produced in LSEP process which was extremely favorable for the subsequent biotransformation of fermentable sugar to other valuable products such as ethanol. Preliminary study showed that the ethanol yield when using the fermentable sugar as carbon source was comparable to that using glucose. The liquid residue of LSEP treated turmeric tuber after diosgenin production can be utilized as a quality fermentable carbon source. Therefore, LSEP has great potential in industrial application in diosgenin clean production and comprehensive utilization of turmeric tuber.
authors: Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, China; Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Molecular Biophysics Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Mi
authors: M
authors: Wei
authors: Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Molecular Biophysics Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
authors: Yao
authors: Y
authors: Tong
authors: School of Life Sciences, Jianghan University, Hubei Province Engineering Research Center for Legume Plants, Wuhan 430056, China.
authors: Hongbo
authors: H
authors: Wang
authors: Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, China.
authors: Lihua
authors: L
authors: Wang
authors: Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Molecular Biophysics Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China; Wuhan Huashite Industrial Biotechnology Development Co., Ltd., Wuhan Institute of Biotechnology, Wuhan 430075, China. Electronic address: yulongjiang@hust.edu.cn.
authors: Longjiang
authors: L
authors: Yu
mesh_terms: D056804:Biofuels; D019149:Bioreactors; D001711:Biotransformation; D030024:Curcuma / Q000737:chemistry / Q000528:radiation effects; D027742:Dioscorea / Q000737:chemistry* / Q000528:radiation effects; D004144:Diosgenin / Q000302:isolation & purification*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin; D008872:Microwaves; D035281:Plant Tubers / Q000737:chemistry / Q000528:radiation effects; D011312:Pressure; D012503:Saponins / Q000737:chemistry; D013227:Steam; D000069453:Ultrasonic Waves
chemical_list: D056804:Biofuels; D012503:Saponins; D013227:Steam; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D004144:Diosgenin
keywords: Comprehensive utilization; Diosgenin yield; Fermentable sugar production; Low-pressure steam expansion pretreatment