title: Newcastle Disease Virus V Protein Targets Phosphorylated STAT1 to Block IFN-I Signaling.
abstract: Newcastle disease virus (NDV) V protein is considered as an effector for IFN antagonism, however, the mechanism remains unknown. In this study, the expression of STAT1 and phospho-STAT1 in cells infected with NDV or transfected with V protein-expressing plasmids were analyzed. Our results showed that NDV V protein targets phospho-STAT1 reduction in the cells depends on the stimulation of IFN-α. In addition, a V-deficient genotype VII recombinant NDV strain rZJ1-VS was constructed using reverse genetic technique to confirm the results. The rZJ1-VS lost the ability to reduce phospho-STAT1 and induced higher expression of IFN-responsive genes in infected cells. Furthermore, treatment with an ubiquitin E1 inhibitor PYR-41 demonstrated that phospho-STAT1 reduction was caused by degradation, but not de-phosphorylation. We conclude that NDV V protein targets phospho-STAT1 degradation to block IFN-α signaling, which adds novel knowledge to the strategies used by paramyxoviruses to evade IFN.
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Xusheng
authors: X
authors: Qiu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.|Key Laboratory of Animal Infectious Diseases, Yangzhou University, Yangzhou, Jiangsu, China.
authors: Qiang
authors: Q
authors: Fu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Chunchun
authors: C
authors: Meng
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Shengqing
authors: S
authors: Yu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Yuan
authors: Y
authors: Zhan
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Luna
authors: L
authors: Dong
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Cuiping
authors: C
authors: Song
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Yingjie
authors: Y
authors: Sun
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.
authors: Lei
authors: L
authors: Tan
authors: Key Laboratory of Animal Infectious Diseases, Yangzhou University, Yangzhou, Jiangsu, China.
authors: Shunlin
authors: S
authors: Hu
authors: Key Laboratory of Animal Infectious Diseases, Yangzhou University, Yangzhou, Jiangsu, China.
authors: Xiaoquan
authors: X
authors: Wang
authors: Key Laboratory of Animal Infectious Diseases, Yangzhou University, Yangzhou, Jiangsu, China.
authors: Xiaowen
authors: X
authors: Liu
authors: Key Laboratory of Animal Infectious Diseases, Yangzhou University, Yangzhou, Jiangsu, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors: Daxin
authors: D
authors: Peng
authors: Key Laboratory of Animal Infectious Diseases, Yangzhou University, Yangzhou, Jiangsu, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors: Xiufan
authors: X
authors: Liu
authors: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
authors: Chan
authors: C
authors: Ding
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D005814:Genes, Viral; D006801:Humans; D016898:Interferon-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009522:Newcastle disease virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D011995:Recombination, Genetic; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*
chemical_list: C070065:36 kDa V protein, Newcastle disease virus; D016898:Interferon-alpha; D050794:STAT1 Transcription Factor; D014764:Viral Proteins