title: Predominant Tubular Interleukin-18 Expression in Polyomavirus-Associated Nephropathy.
abstract: Polyomavirus-associated nephropathy (PVAN) occurs in a significant percentage of renal transplant recipients, with BK virus reactivation as the main causative agent. PVAN leads to tubular damage and may result in allograft loss. In this study, we analyzed the antiviral immune response in PVAN. Transcription of the proinflammatory cytokine interleukin-18 (IL-18) was significantly higher in PVAN biopsies compared with T cell-mediated rejection (TCMR) (1.42 ± 0.20 and 0.69 ± 0.10, respectively; *P = 0.0021). Tubular expression of IL-18 was significantly increased in PVAN compared with TCMR (2.00 ± 0.24 and 1.333 ± 0.13, respectively; *P = 0.028). In contrast, in TCMR, IL-18 was expressed predominantly by CD163-positive macrophages. These data suggest that the antiviral immune response in PVAN is partly coordinated by the tubular epithelium, whereas in TCMR, this may be controlled by inflammatory cells.
authors: 1 Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands. 2 Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands. 3 Department of Nephrology, Radboud University Medical Center, Nijmegen, The Netherlands. 4 Department of Nephrology, Academic Medical Center, Amsterdam, The Netherlands.
authors: Geurt
authors: G
authors: Stokman
authors: Jesper
authors: J
authors: Kers
authors: Ünsal
authors: Ü
authors: Yapici
authors: Joris J
authors: JJ
authors: Hoelbeek
authors: Nike
authors: N
authors: Claessen
authors: Onno J
authors: OJ
authors: de Boer
authors: Mihai G
authors: MG
authors: Netea
authors: Luuk
authors: L
authors: Hilbrands
authors: Fréderike J
authors: FJ
authors: Bemelman
authors: Ineke J M
authors: IJ
authors: Ten Berge
authors: Sandrine
authors: S
authors: Florquin
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001739:BK Virus; D005260:Female; D006084:Graft Rejection; D006801:Humans; D020382:Interleukin-18 / Q000032:analysis / Q000235:genetics*; D007674:Kidney Diseases / Q000276:immunology*; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D027601:Polyomavirus Infections / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000032:analysis; D014158:Transcription, Genetic; D014412:Tumor Virus Infections / Q000276:immunology*; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D020382:Interleukin-18; D011956:Receptors, Cell Surface