title: A Colloidal Stability Assay Suitable for High-Throughput Screening.
abstract: A library of 32 polystyrene copolymer latexes, with diameters ranging between 53 and 387 nm, was used to develop and demonstrate a high-throughput assay using a 96-well microplate platform to measure critical coagulation concentrations, a measure of colloidal stability. The most robust assay involved an automated centrifugation-decantation step to remove latex aggregates before absorbance measurements, eliminating aggregate interference with optical measurements made through the base of the multiwell plates. For smaller nanoparticles (diameter <150 nm), the centrifugation-decantation step was not required as the interference was less than with larger particles. Parallel measurements with a ChemiDoc MP plate scanner gave indications of aggregation; however, the results were less sensitive than the absorbance measurements.
authors: Department of Chemical Engineering, McMaster University , Hamilton, Ontario Canada L8S4L8.
authors: Carla
authors: C
authors: Abarca
authors: Department of Chemical Engineering, McMaster University , Hamilton, Ontario Canada L8S4L8.
authors: M Monsur
authors: MM
authors: Ali
authors: Department of Chemical Engineering, McMaster University , Hamilton, Ontario Canada L8S4L8.
authors: Songtao
authors: S
authors: Yang
authors: Department of Chemical Engineering, McMaster University , Hamilton, Ontario Canada L8S4L8.
authors: Xiaofei
authors: X
authors: Dong
authors: Department of Chemical Engineering, McMaster University , Hamilton, Ontario Canada L8S4L8.
authors: Robert H
authors: RH
authors: Pelton