title: Senescence associated secretory phenotype profile from primary lung mice fibroblasts depends on the senescence induction stimuli.
abstract: Cellular senescence is a multifactorial phenomenon of growth arrest and distorted function, which has been recognized as an important feature during tumor suppression mechanisms and a contributor to aging. Senescent cells have an altered secretion pattern called Senescence-Associated Secretory Phenotype (SASP) that comprises a complex mix of factors including cytokines, growth factors, chemokines, and matrix metalloproteinases. SASP has been related with local inflammation that leads to cellular transformation and neurodegenerative diseases. Various pathways for senescence induction have been proposed; the most studied is replicative senescence due to telomere attrition called replicative senescence (RS). However, senescence can be prematurely achieved when cells are exposed to diverse stimuli such as oxidative stress (stress-induced premature senescence, SIPS) or proteasome inhibition (proteasome inhibition-induced premature senescence, PIIPS). SASP has been characterized in RS and SIPS but not in PIIPS. Hence, our aim was to determine SASP components in primary lung fibroblasts obtained from CD-1 mice induced to senescence by PIIPS and compare them to RS and SIPS. Our results showed important variations in the 62 cytokines analyzed, while SIPS and RS showed an increase in the secretion of most cytokines, and in PIIPS only 13 were incremented. Variations in glutathione-redox balance were also observed in SIPS and RS, and not in PIIPS. All senescence types SASP displayed a pro-inflammatory profile and increased proliferation in L929 mice fibroblasts exposed to SASP. However, the behavior observed was not exactly the same, suggesting that the senescence induction pathway might encompass dissimilar responses in adjacent cells and promote different outcomes.
authors: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, AP 55-535, México D.F., 09340, Mexico.|Posgrado en Biología Experimental., México D.F., Mexico.
authors: L A
authors: LA
authors: Maciel-Barón
authors: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, AP 55-535, México D.F., 09340, Mexico.|Posgrado en Biología Experimental., México D.F., Mexico.
authors: S L
authors: SL
authors: Morales-Rosales
authors: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, AP 55-535, México D.F., 09340, Mexico.
authors: A A
authors: AA
authors: Aquino-Cruz
authors: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, AP 55-535, México D.F., 09340, Mexico.
authors: F
authors: F
authors: Triana-Martínez
authors: Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, SSA, México D.F., 14269, Mexico.
authors: S
authors: S
authors: Galván-Arzate
authors: Departamento de Investigación Básica, Instituto Nacional de Geriatría, SSA, México, D.F., 14080, Mexico.
authors: A
authors: A
authors: Luna-López
authors: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, AP 55-535, México D.F., 09340, Mexico.
authors: V Y
authors: VY
authors: González-Puertos
authors: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, AP 55-535, México D.F., 09340, Mexico.
authors: N E
authors: NE
authors: López-Díazguerrero
authors: Department of Pathology and Laboratory Medicine, Drexel University College of Medicine, Philadelphia, PA, 19102, USA.
authors: C
authors: C
authors: Torres
authors: Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana Iztapalapa, AP 55-535, México D.F., 09340, Mexico. mkf@xanum.uam.mx.
authors: Mina
authors: M
authors: Königsberg
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism*; D004249:DNA Damage; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D008168:Lung / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D010641:Phenotype; D016615:Telomere / Q000378:metabolism
chemical_list: D016207:Cytokines
keywords: Aging; Cytokines; Inflammation; Oxidative stress; Proteasome; SASP; Senescence