title: Synthesis, characterization and in vitro evaluation of exquisite targeting SPIONs-PEG-HER in HER2+ human breast cancer cells.
abstract: A stable, biocompatible and exquisite SPIONs-PEG-HER targeting complex was developed. Initially synthesized superparamagnetic iron oxide nanoparticles (SPIONs) were silanized using 3-aminopropyltrimethoxysilane (APS) as the coupling agent in order to allow the covalent bonding of polyethylene glycol (PEG) to the SPIONs to improve the biocompatibility of the SPIONs. SPIONs-PEG were then conjugated with herceptin (HER) to permit the SPIONs-PEG-HER to target the specific receptors expressed over the surface of the HER2+ metastatic breast cancer cells. Each preparation step was physico-chemically analyzed and characterized by a number of analytical methods including AAS, FTIR spectroscopy, XRD, FESEM, TEM, DLS and VSM. The biocompatibility of SPIONs-PEG-HER was evaluated in vitro on HSF-1184 (human skin fibroblast cells), SK-BR-3 (human breast cancer cells, HER+), MDA-MB-231 (human breast cancer cells, HER-) and MDA-MB-468 (human breast cancer cells, HER-) cell lines by performing MTT and trypan blue assays. The hemolysis analysis results of the SPIONs-PEG-HER and SPIONs-PEG did not indicate any sign of lysis while in contact with erythrocytes. Additionally, there were no morphological changes seen in RBCs after incubation with SPIONs-PEG-HER and SPIONs-PEG under a light microscope. The qualitative and quantitative in vitro targeting studies confirmed the high level of SPION-PEG-HER binding to SK-BR-3 (HER2+ metastatic breast cancer cells). Thus, the results reflected that the SPIONs-PEG-HER can be chosen as a favorable biomaterial for biomedical applications, chiefly magnetic hyperthermia, in the future.
authors: Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, Skudai 81310, Johor Bahru, Johor, Malaysia.
authors: Javad Hamzehalipour
authors: JH
authors: Almaki
authors: Rozita
authors: R
authors: Nasiri
authors: Ani
authors: A
authors: Idris
authors: Fadzilah Adibah Abdul
authors: FA
authors: Majid
authors: Mojtaba
authors: M
authors: Salouti
authors: Tet Soon
authors: TS
authors: Wong
authors: Shadab
authors: S
authors: Dabagh
authors: Mohsen
authors: M
authors: Marvibaigi
authors: Neda
authors: N
authors: Amini
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D000067493:Dynamic Light Scattering; D005260:Female; D005290:Ferric Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D006461:Hemolysis / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011092:Polyethylene Glycols; D018719:Erb-b2 Receptor Tyrosine Kinases; C000499:ferric oxide; C508053:ERBB2 protein, human