title: X and Y chromosome-bearing spermatozoa are equally able to uptake and internalize exogenous DNA by sperm-mediated gene transfer in swine.
abstract: Since proteomic differences between male X/Y chromosome-bearing gametes have recently been described, a question has been raised: could these differences be responsible for different behavior between X and Y chromosome-bearing spermatozoa during the binding and internalization of exogenous DNA in the swine species? In order to investigate this hypothesis, our group studied the process of the uptake and internalization of exogenous DNA in X and Y chromosome-bearing sperm sub-populations. No significant differences were found between sperm types in both the uptake and internalization of exogenous DNA. The quantity of internalized exogenous DNA was significantly lower than that of the uptaken DNA. In conclusion, our results showed that X and Y chromosomes-bearing spermatozoa have the same binding capacity and internalization of DNA, and the proteomic differences between them do not seem to interfere with these complex processes.
authors: Department of Veterinary Medical Sciences - DIMEVET, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
authors: Andrea
authors: A
authors: Zaniboni
authors: Department of Veterinary Medical Sciences - DIMEVET, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
authors: Marcella
authors: M
authors: Spinaci
authors: Department of Veterinary Medical Sciences - DIMEVET, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
authors: Augusta
authors: A
authors: Zannoni
authors: Department of Veterinary Medical Sciences - DIMEVET, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
authors: Chiara
authors: C
authors: Bernardini
authors: Department of Veterinary Medical Sciences - DIMEVET, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
authors: Monica
authors: M
authors: Forni
authors: Department of Veterinary Medical Sciences - DIMEVET, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy. Electronic address: marialaura.bacci@unibo.it.
authors: Maria Laura
authors: ML
authors: Bacci
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D008297:Male; D020543:Proteome; D013094:Spermatozoa / Q000378:metabolism*; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism*; D014960:X Chromosome / Q000378:metabolism*; D014998:Y Chromosome / Q000378:metabolism*
chemical_list: D020543:Proteome; D004247:DNA
keywords: Differences; SMGT; Sperm sorting; Swine; X/Y chromosome-bearing spermatozoa