title: Effect of vitamin D3 on maturation and antigen-presenting function of dendritic cells treated with Mycobacterium tuberculosis.
abstract: OBJECTIVE
  To investigate the phenotypic characteristics and functional capability differences of mouse bone marrow-derived dendritic cells after stimulation with Mycobacterium tuberculosis in the presence or absence of vitamin D3.
  
  
  METHODS
  Mouse bone marrow-derived cells were cultured with GM-CSF (20 ng/mL). Then, one was added with 100 nmol/L of 25(OH)D3, while the other did not. On day 6, 5 μg/mL of BCG was added to stimulate the cells for 24 h. On day 7, suspension cells were harvested for phenotypic and functional analyses.
  
  
  RESULTS
  The percentages of CD86 dendritic cells (DCs) in the control group and 25(OH)D3 group were 66.97% ± 8.29% and 52.18% ± 8.52%, respectively; the mean fluorescence intensities of MHC-II in the control group and 25(OH)D3 group were 1 102.16 ± 371.02 and 681.62 ± 292.71. The expression levels of MHC- II and CD86 on the surface of the DCs in 25(OH)D3 group were significantly lower than those of the control group. The ability of the DCs to stimulate proliferation of T-lymphocytes was also significantly lower than that of the control group.
  
  
  CONCLUSIONS
  These findings suggest that 25(OH)D3 modulates the immune response by affecting the maturation and function of DCs in Mycobacterium tuberculosis period.
authors: No 202 Hospital of People's Liberation Army, Liaoning 110003, China.
authors: Chun-Yu
authors: CY
authors: Liu
authors: Department of Orthopedics, Southwest Hospital, Third Military Medical, Chongqing 400038, China.
authors: Ze-Hua
authors: ZH
authors: Zhang
authors: Department of Orthopedics, General Hospital of Shenyang Military, Liaoning 110016, China.
authors: Hui-Feng
authors: HF
authors: Yang
authors: No 202 Hospital of People's Liberation Army, Liaoning 110003, China.
authors: Hui
authors: H
authors: Xu
authors: Department of Orthopedics, Southwest Hospital, Third Military Medical, Chongqing 400038, China.
authors: Fei-Fan
authors: FF
authors: Cheng
authors: Department of Orthopedics, Southwest Hospital, Third Military Medical, Chongqing 400038, China. Electronic address: xjzslw@163.com.
authors: Jian-Zhong
authors: JZ
authors: Xu
keywords: 25(OH)D3; Dendritic cells; Mycobacterium tuberculosis