title: A Simple, Reproducible, Inexpensive, Yet Old-Fashioned Method for Determining Phagocytic and Bactericidal Activities of Macrophages.
abstract: Macrophages (Mφ) play a pivotal role in the protection system by recognizing and eliminating invading pathogenic bacteria. Phagocytosis and the killing of invading bacteria are major effector functions of Mφ. Although the phagocytic and bactericidal activities of Mφ have been analyzed via several methods using a light microscope, a fluorescence microscope, or a fluorescence-activated cell sorter, expensive materials and equipment are usually required, and the methods are rather complicated. Moreover, it is impossible to determine both the phagocytic and bactericidal activities of Mφ simultaneously using these methods. In this review, we describe a simple, reproducible, inexpensive, yet old-fashioned method (antibiotic protection assay) for determining the phagocytic and bactericidal activities of Mφ.
authors: Laboratory of Immunology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan.
authors: Masakazu
authors: M
authors: Kaneko
authors: Laboratory of Immunology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan.
authors: Yoshiko
authors: Y
authors: Emoto
authors: Laboratory of Immunology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan. masashi.emoto@gmail.com.
authors: Masashi
authors: M
authors: Emoto
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins
keywords: Anti-bacterial agent; gentamicin; macrophage; phagocytosis