title: Tumor cell-derived secretory factor downregulates Semaphorin-3a in osteoblasts by activating mammalian target of rapamycin pathway.
abstract: We found that conditioned medium derived from Lewis Lung Carcinoma cells down-regulated Semaphorin3a (Sema3a) mRNA expression and increased the activity of mammalian target of rapamycin complex 1 (mTORC1) in osteoblast-like MC3T3-E1 cells. Furthermore, mTORC1 inhibition with rapamycin counteracted the effect of conditioned media on Sema3a mRNA expression. These results suggest that tumor cells decrease Sema3a mRNA expression in osteoblast in an mTORC1-dependent manner.
authors: a Faculty of Pharmacy, Department of Pharmacology , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Daisuke
authors: D
authors: Yamada
authors: a Faculty of Pharmacy, Department of Pharmacology , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Kohichi
authors: K
authors: Kawahara
authors: b Faculty of Pharmacy, Department of Toxicology , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Masanobu
authors: M
authors: Ozaki
authors: a Faculty of Pharmacy, Department of Pharmacology , Niigata University of Pharmacy and Applied Life Sciences , Niigata , Japan.
authors: Takehiko
authors: T
authors: Maeda
mesh_terms: D000818:Animals; D018827:Carcinoma, Lewis Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D000076222:Mechanistic Target of Rapamycin Complex 1; D051379:Mice; D046912:Multiprotein Complexes / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D040121:Semaphorin-3A / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D046912:Multiprotein Complexes; D012333:RNA, Messenger; C108639:SEMA3A protein, human; D040121:Semaphorin-3A; D000076222:Mechanistic Target of Rapamycin Complex 1; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus
keywords: Semaphorin-3a; mTORC1; osteoblast