title: Identification of Lactobacillus proteins with different recognition patterns between immune rabbit sera and nonimmune mice or human sera.
abstract: BACKGROUND
  The genus Lactobacillus belongs to a large heterogeneous group of low G + C Gram-positive anaerobic bacteria, which are frequently used as probiotics. The health-beneficial effects, in particular the immunomodulation effect, of probiotics depend on the strain and dose used. Strain variations may be related to diversity of the cell surface architecture of bacteria and the ability to express specific antigens or secrete compounds. The use of Lactobacillus as probiotic requires a comprehensive understanding of its effect on host immune system. To evaluate the potential immunoreactive properties of proteins isolated from four Lactobacillus strains: L. johnsonii 142 and L. johnsonii 151, L. rhamnosus LOCK 0900 and L. casei LOCK 0919, the polyclonal sera obtained from mouse and human have been tested as well as with sera from rabbits immunized with whole lactobacilli cells.
  
  
  RESULTS
  The reactivity of isolated proteins detected by SDS-PAGE and Western blotting was heterogeneous and varied between different serum samples. The proteins with the highest immunoreactivity were isolated, purified and sequenced, in particular the fractions were identified as phosphoglycerate kinase (L. johnsonii 142), glyceraldehyde 3-phosphate dehydrogenase (L. johnosnii 142, L. rhamnosus LOCK 0900), hypothetic protein JDM1_1307 (L. johnsonii 151) and fructose/tagatose-bisphosphate-aldolase (L. casei LOCK 0919).
  
  
  CONCLUSIONS
  The different prevalence of reactions against tested antigens in rabbit, mouse and human sera may indicate significant differences in immune system and commensal cross-talk in these groups. The identification of immunoreactive lactobacilli proteins opens the possibility to use them as an antigens for development of vaccines.
authors: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, Wroclaw, Poland. sabina.gorska@iitd.pan.wroc.pl.
authors: Sabina
authors: S
authors: Górska
authors: Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, Faculty of Food Science, Wroclaw, Poland.
authors: Barbara
authors: B
authors: Buda
authors: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, Wroclaw, Poland.
authors: Ewa
authors: E
authors: Brzozowska
authors: Institute of Microbiology, Laboratory of Gnotobiology, Academy of Sciences of the Czech Republic v. v. i., 549 22, Novy Hradek, Czech Republic.
authors: Martin
authors: M
authors: Schwarzer
authors: Institute of Microbiology, Laboratory of Gnotobiology, Academy of Sciences of the Czech Republic v. v. i., 549 22, Novy Hradek, Czech Republic.
authors: Dagmar
authors: D
authors: Srutkova
authors: Institute of Microbiology, Laboratory of Gnotobiology, Academy of Sciences of the Czech Republic v. v. i., 549 22, Novy Hradek, Czech Republic.
authors: Hana
authors: H
authors: Kozakova
authors: Department of Medical Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, Wroclaw, Poland.
authors: Andrzej
authors: A
authors: Gamian
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000032:analysis* / Q000276:immunology; D007778:Lactobacillus / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D051379:Mice; D011817:Rabbits
chemical_list: D001426:Bacterial Proteins; D007106:Immune Sera