title: Circulating 'lncRNA OTTHUMT00000387022' from monocytes as a novel biomarker for coronary artery disease.
abstract: Long non-coding RNAs (lncRNAs) have been found to be involved in the pathogenesis of coronary artery disease (CAD). However, it remains to be established whether or not circulating lncRNAs can serve as biomarkers of CAD.
  
  
  
  Using a microarray-based lncRNA expression profiling, we found 86 lncRNAs that were differentially expressed in circulating peripheral blood monocytes and plasma from 15 CAD patients and 15 control subjects. After choosing a consistent criterion (average normalized intensity ≥7 with significance <0.005) and confirmed by quantitative PCR, only three lncRNAs (CoroMarker, BAT5, and IL21R-AS1) remained as candidate CAD biomarkers. Using the analysis of area under the curve (AUC) of the receiver-operating characteristic in another pilot group and another larger cohort, CoroMarker was found to be the best candidate biomarker for CAD with an AUC of 0.920 and 95% confidence interval of 0.892-0.947. CoroMarker was independent from known CAD risk factors and other cardiovascular diseases. In a prospective study, we found that the sensitivity and specificity of CoroMarker were 76 and 92.5%, respectively. Functional enrichment analysis showed CoroMarker to be clustered with genes positively associated with signal transduction, transmembrane transport, synaptic transmission, and innate immunity and negatively associated with inflammation. These findings were validated in THP-1 cells; CoroMarker siRNA treatment decreased the concentrations of proinflammatory cytokines [interleukin (IL)-1β, IL-6, and tumour necrosis factor α] in the culture medium.
  
  
  
  The present study suggests that CoroMarker is a novel and specific biomarker of CAD.
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Yue
authors: Y
authors: Cai
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.|Department of Neurology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.
authors: Yujia
authors: Y
authors: Yang
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Cardiovascular Research Center and Department of Physiology, Temple University School of Medicine, Philadelphia, PA, USA.
authors: Xiongwen
authors: X
authors: Chen
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Genze
authors: G
authors: Wu
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Xiaoqun
authors: X
authors: Zhang
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Yukai
authors: Y
authors: Liu
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Junyi
authors: J
authors: Yu
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Xinquan
authors: X
authors: Wang
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Jinjuan
authors: J
authors: Fu
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Chuanwei
authors: C
authors: Li
authors: Division of Nephrology, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.|Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA.
authors: Pedro A
authors: PA
authors: Jose
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China linzhou007@126.com chunyuzeng167@163.com.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Chunyu
authors: C
authors: Zeng
authors: Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China linzhou007@126.com chunyuzeng167@163.com.|Chongqing Institute of Cardiology, Chongqing, P.R. China.
authors: Lin
authors: L
authors: Zhou
mesh_terms: D000368:Aged; D019540:Area Under Curve; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D017077:Culture Media, Conditioned / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D056726:Genetic Association Studies; D005819:Genetic Markers; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011446:Prospective Studies; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000097:blood* / Q000235:genetics; D012372:ROC Curve; D015203:Reproducibility of Results; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D005819:Genetic Markers; C508585:IL1B protein, human; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D062085:RNA, Long Noncoding; D014409:Tumor Necrosis Factor-alpha; C000623211:long non-coding RNA OTTHUMT00000387022, human
keywords: Biomarker; Blood monocyte; Coronary artery disease; Long non-coding RNAs