title: Systematic characterization of the metabolites of echinacoside and acteoside from Cistanche tubulosa in rat plasma, bile, urine and feces based on UPLC-ESI-Q-TOF-MS.
abstract: Echinacoside (ECH) and acteoside (ACT), as the most and major active components of Cistanche tubulosa, were reported to possess cardioactive, neuroprotective and hepatocyte protective effects, as well as antibacterial, antioxidative effects. Recently, more studies have focused on their pharmacological activities. However, their metabolic profiles in vivo have not been sufficiently investigated. This study proposes an approach for rapidly identifying the complicated and unpredictable metabolites of ECH and ACT in rat plasma, bile, urine and feces, and systematically and comprehensively revealing their major metabolic pathways, based on powerful ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Plasma, bile, urine and feces were collected from rats after a single 200 mg/kg oral dose. A total of 49 metabolites were detected in rat biological samples. Through analyzing metabolites in bile samples, it was found that ECH and ACT were subjected to a marked hepatic first-pass effect in liver. Copyright © 2016 John Wiley & Sons, Ltd.
authors: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
authors: Qingling
authors: Q
authors: Cui
authors: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
authors: Yingni
authors: Y
authors: Pan
authors: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
authors: Xuewei
authors: X
authors: Bai
authors: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
authors: Wei
authors: W
authors: Zhang
authors: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
authors: Lixia
authors: L
authors: Chen
authors: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China.
authors: Xiaoqiu
authors: X
authors: Liu
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000097:blood / Q000652:urine; D005960:Glucosides / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D059808:Polyphenols
chemical_list: D005419:Flavonoids; D005960:Glucosides; D010636:Phenols; C517561:echinoside; C058956:acteoside; D059808:Polyphenols
keywords: UPLC/Q-TOF-MS; acteoside; cistanche tubulosa; echinacoside; metabolic profile; rat