title: Investigation of three lignin complexes with antioxidant and immunological capacities from Inonotus obliquus.
abstract: Mushroom Inonotus obliquus (I. obliquus), a folk medicine, has been widely used to treat several human malicious tumors since 16th century. In this study, three homogenous biomolecules (designated IOA1, IOA2 and IOA3) were prepared from the alkali extract of I. obliquus. Their molecular weights were measured to be 6.1 × 10(4), 2.9 × 10(4) and 3.5 × 10(4) g/mol respectively and all of them were characterized as lignin-carbohydrate complexes mainly comprised lignin as well as -25% carbohydrates. Antioxidant assays indicated that all of them exhibited pronounced reductive power and strong scavenging activities on DPPH and hydroxyl radicals in vitro. Immunological tests showed that they could also significantly stimulate nitric oxide production and phagocytic activity in RAW 264.7 macrophages. These results implied that the lignin-carbohydrate complexes extracted from I. obliquus might be used as novel natural antioxidants or immunostimulants in functional foods or pharmaceutical candidates.
authors: College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Hong
authors: H
authors: Niu
authors: College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Dan
authors: D
authors: Song
authors: College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Haibo
authors: H
authors: Mu
authors: College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Wuxia
authors: W
authors: Zhang
authors: College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors: Feifei
authors: F
authors: Sun
authors: College of Science, Northwest A & F University, Yangling 712100, Shaanxi, China. Electronic address: jduan@nwsuaf.edu.cn.
authors: Jinyou
authors: J
authors: Duan
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D050260:Carbohydrate Metabolism; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D007501:Iron / Q000737:chemistry; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D000067996:RAW 264.7 Cells; D012995:Solubility
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D007155:Immunologic Factors; D010851:Picrates; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D008031:Lignin; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron
keywords: Antioxidant activity; Immunological activity; Lignin-carbohydrate complexes