title: A specialist herbivore pest adaptation to xenobiotics through up-regulation of multiple Cytochrome P450s.
abstract: The adaptation of herbivorous insects to their host plants is hypothesized to be intimately associated with their ubiquitous development of resistance to synthetic pesticides. However, not much is known about the mechanisms underlying the relationship between detoxification of plant toxins and synthetic pesticides. To address this knowledge gap, we used specialist pest Colorado potato beetle (CPB) and its host plant, potato, as a model system. Next-generation sequencing (454 pyrosequencing) was performed to reveal the CPB transcriptome. Differential expression patterns of cytochrome P450 complement (CYPome) were analyzed between the susceptible (S) and imidacloprid resistant (R) beetles. We also evaluated the global transcriptome repertoire of CPB CYPome in response to the challenge by potato leaf allelochemicals and imidacloprid. The results showed that more than half (51.2%) of the CBP cytochrome P450 monooxygenases (P450s) that are up-regulated in the R strain are also induced by both host plant toxins and pesticide in a tissue-specific manner. These data suggest that xenobiotic adaptation in this specialist herbivore is through up-regulation of multiple P450s that are potentially involved in detoxifying both pesticide and plant allelochemicals.
authors: Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.|Department of Entomology, Washington State University, Pullman, WA 99164, USA.
authors: Fang
authors: F
authors: Zhu
authors: Department of Chemistry, Washington State University, Pullman 99164, USA.
authors: Timothy W
authors: TW
authors: Moural
authors: Department of Microbiology, Immunology and Biochemistry, University of Tennessee, Memphis, TN 38163, USA.
authors: David R
authors: DR
authors: Nelson
authors: Department of Entomology, University of Kentucky, Lexington, KY 40546, USA.
authors: Subba R
authors: SR
authors: Palli
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D001517:Coleoptera / Q000502:physiology*; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D060434:Herbivory / Q000187:drug effects* / Q000235:genetics; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D015262:Xenobiotics / Q000494:pharmacology*
chemical_list: D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System