title: Cultivation of Scenedesmus obliquus in liquid hydrolysate from flash hydrolysis for nutrient recycling.
abstract: The production of biofuels from microalgae is associated with high demands of nutrients (nitrogen and phosphorus) required for growth. Recycling nutrients from the residual biomass is essential to obtain a sustainable production. In this work, the aqueous phase obtained from flash hydrolysis of Scenedesmus sp. was used as cultivation medium for a microalga of the same genus, to assess the feasibility of this technique for nutrient recycling purposes. Batch and continuous cultivations were carried out, to determine growth performances in this substrate compared to standard media, and verify if a stable biomass production could be obtained. In continuous experiments, the effect of hydrolysate inlet concentration and of residence time were assessed to optimize nutrient supply in relation to productivity. Results obtained show that nutrient recycling is feasible by treating biomass with flash hydrolysis, and Scenedesmus is capable of recycling large amounts of recovered nutrients.
authors: Department of Industrial Engineering DII, University of Padova, Via Marzolo 9, 35131 Padova, Italy. Electronic address: elena.barbera@studenti.unipd.it.
authors: Elena
authors: E
authors: Barbera
authors: Department of Industrial Engineering DII, University of Padova, Via Marzolo 9, 35131 Padova, Italy.
authors: Eleonora
authors: E
authors: Sforza
authors: Department of Civil and Environmental Engineering, Old Dominion University, Norfolk, VA 23529, United States.
authors: Sandeep
authors: S
authors: Kumar
authors: Department of Biology, University of Padova, Via U. Bassi 58/B, 35121 Padova, Italy.
authors: Tomas
authors: T
authors: Morosinotto
authors: Department of Industrial Engineering DII, University of Padova, Via Marzolo 9, 35131 Padova, Italy.
authors: Alberto
authors: A
authors: Bertucco
mesh_terms: D061249:Batch Cell Culture Techniques; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D006868:Hydrolysis; D058086:Microalgae / Q000254:growth & development; D009584:Nitrogen / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D058433:Photobioreactors / Q000382:microbiology; D059027:Recycling; D044444:Scenedesmus / Q000254:growth & development*; D013997:Time Factors
chemical_list: D010758:Phosphorus; D009584:Nitrogen
keywords: Continuous photobioreactor; Flash hydrolysis; Microalgae; Mixotrophy