title: Rabbit olfactory stem cells. Isolation protocol and characterization.
abstract: OBJECTIVE
  To describe a new technique for isolation of a mesenchymal stem cells (MSCs) population from the olfactory mucosa in rabbits.
  
  
  METHODS
  Olfactory stem cells (OSCs) were retrieved from under the cribriform plate of the Ethmoid bone. Several assays were accomplished to characterize the cell population and attest its viability in vitro. The cells were submitted to flow cytometry with the antibodies CD34, CD45, CD73, CD79, CD90 and CD105 and also they were induced to differentiate in three lineages. Functional evaluation involved analysis of in vitro growth behavior, colony forming unit like fibroblasts (CFU-f) and cryopreservation response. Further transduction with Green Fluorescent Protein (GFP) was also performed.
  
  
  RESULTS
  The OSCs showed mesenchymal features, as positive response to CD34, CD73 and CD90 antibodies and plasticity. Additionally, these cells have high proliferated rate, and they could be cultured through many passages and kept the ability to proliferate and differentiate after cryopreservation. The positive response to the transduction signalizes the possibility of cellular tracking in vivo. This is a desirable feature in case those cells are used for pre-clinical trials.
  
  
  CONCLUSIONS
  The cells harvested were mesenchymal stem cells and the technique described is therefore efficient for rabbit olfactory stem cells isolation.
authors: Veterinary Medicine Department, Faculty of Animal Sciences and Food Engineering, Universidade de Sao Paulo, Pirassununga, SP, Brazil.
authors: Anna Carolina Mazeto
authors: AC
authors: Ercolin
authors: Faculty of Animal Sciences and Food Engineering, USP, Pirassununga, SP, Brazil.
authors: Kelly Cristine Santos
authors: KC
authors: Roballo
authors: Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, SP, Brazil.
authors: Juliana Barbosa
authors: JB
authors: Casals
authors: Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, SP, Brazil.
authors: Naira Caroline Godoy
authors: NC
authors: Pieri
authors: Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, SP, Brazil.
authors: Aline Fernanda
authors: AF
authors: Souza
authors: Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, SP, Brazil.
authors: Rodrigo da Silva Nunes
authors: Rda S
authors: Barreto
authors: Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, SP, Brazil.
authors: Fabiana Fernandes
authors: FF
authors: Bressan
authors: Universidade Federal do Piauí, Teresina, PI, Brazil.
authors: Matheus Levi Tajra
authors: ML
authors: Feitosa
authors: Faculty of Veterinary Medicine and Animal Science, USP, Sao Paulo, SP, Brazil.
authors: Maria Angélica
authors: MA
authors: Miglino
authors: Faculty of Animal Sciences and Food Engineering, USP, Pirassununga, SP, Brazil.
authors: Flávio Vieira
authors: FV
authors: Meirelles
authors: Faculty of Animal Sciences and Food Engineering, USP, Pirassununga, SP, Brazil.
authors: Carlos Eduardo
authors: CE
authors: Ambrósio
mesh_terms: D015720:5'-Nucleotidase / Q000502:physiology; D000818:Animals; D018952:Antigens, CD34 / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D000066670:Cell Plasticity / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D005004:Ethmoid Bone / Q000166:cytology; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009831:Olfactory Mucosa / Q000166:cytology* / Q000254:growth & development; D011817:Rabbits; D018800:Thy-1 Antigens / Q000502:physiology
chemical_list: D018952:Antigens, CD34; D018800:Thy-1 Antigens; D049452:Green Fluorescent Proteins; D015720:5'-Nucleotidase