title: [A Simple and Efficient Method of Inducing Targeted Deletions in the Drosophila Genome].
abstract: Deletion mutagenesis is one of the most efficient approaches to studying gene function. However, conventional methods of inducing targeted mutations in the drosophila genome are time- and labor-consuming. This work proposes a new, simple, and effective method of producing drosophila mutants with gene deletions. The method involves the insertion of I-Scel and I-CreI recognition sites and a fragment homologous to the target sequence into the chromosome region of interest by means of an attB-containing construct, the induction of double-strand DNA breaks by the appropriate meganuclease, and their repair by homologous recombination. The procedure results in a deletion extending from the attP-site to the target locus. A cassette was designed to enable single-step construct production for the deletion of any given genomic region. A set of markers facilitates the selection of recombination events. The efficacy of the proposed technique was confirmed by the induction of a 47-kb deletion containing the qtc gene.
authors: O I
authors: OI
authors: Kravchuk
authors: V S
authors: VS
authors: Mikhailov
authors: M Yu
authors: MY
authors: Savitsky
mesh_terms: D000818:Animals; D053960:DNA Breaks; D004331:Drosophila melanogaster; D017353:Gene Deletion; D049750:Genome, Insect; D016296:Mutagenesis; D059767:Recombinational DNA Repair