title: Morin suppresses inflammatory cytokine expression by downregulation of nuclear factor-κB and mitogen-activated protein kinase (MAPK) signaling pathways in lipopolysaccharide-stimulated primary bovine mammary epithelial cells.
abstract: Morin, a flavonoid isolated from Chinese herbs of the Moraceae family, has been reported to possess antiinflammatory activity. However, the effects of morin on mastitis have not been investigated. The present study was conducted to elucidate the antiinflammatory properties of morin on lipopolysaccharide (LPS)-stimulated primary bovine mammary epithelial cells (bMEC). The viability of bMEC was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] assay. Subsequently, bMEC were stimulated with LPS in the presence or absence of morin. Gene expression of proinflammatory cytokines was determined by quantitative real-time PCR (qRT-PCR). Nuclear factor-κB (NF-κB), inhibitory kappa B (IκBα) protein, extracellular signal-regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) were detected by Western blotting. The results showed that cell viability was not affected by morin. Moreover, morin inhibited the gene expression of tumor necrosis factor-α (TNF-α), IL-6, and IL-1β in LPS-stimulated bMEC in a dose-dependent manner. Western blot analysis showed that morin suppressed the phosphorylation of IκBα, NF-κB unit p65, ERK, p38, and JNK in LPS-stimulated bMEC. In conclusion, the protective effects of morin on LPS-induced inflammatory response in bMEC may be due to its ability to suppress NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways. These findings suggest that morin may be used as antiinflammatory drug for mastitis.
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China.
authors: Jingjing
authors: J
authors: Wang
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China.
authors: Changming
authors: C
authors: Guo
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China.
authors: Zhengkai
authors: Z
authors: Wei
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China.
authors: Xuexiu
authors: X
authors: He
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China.
authors: Jinhua
authors: J
authors: Kou
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China.
authors: Ershun
authors: E
authors: Zhou
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China. Electronic address: yangzhengtao01@sina.com.
authors: Zhengtao
authors: Z
authors: Yang
authors: College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, P. R. China. Electronic address: fuyunhesky@sina.com.
authors: Yunhe
authors: Y
authors: Fu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle; D016207:Cytokines / Q000235:genetics*; D015536:Down-Regulation; D004847:Epithelial Cells / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007249:Inflammation / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008414:Mastitis, Bovine / Q000188:drug therapy; D016328:NF-kappa B / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D047309:Flavones
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D005419:Flavonoids; D053583:Interleukin-1beta; D015850:Interleukin-6; D048031:JNK Mitogen-Activated Protein Kinases; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C008548:morin; D047309:Flavones
keywords: cytokine; inflammation; mammary epithelial cells; morin