title: In vitro studies on human periodontal ligament stem cell sheets enhanced by enamel matrix derivative.
abstract: Numerous preclinical and clinical studies have focused on the periodontal regenerative functions of enamel matrix derivative (EMD), a heat-treated preparation derived from enamel matrix proteins (EMPs) of developing porcine teeth. In this study, periodontal ligament (PDL) stem cells (PDLSCs) were isolated, and the effects of EMD on the extracorporeal induction process and the characteristics of PDLSC sheets were investigated for their potential as a more effective stem-cell therapy. EMD-enhanced cell sheets could be induced by complete medium supplemented with 50 μg/mL vitamin C and 100 μg/mL EMD. The EMD-enhanced cell sheets appeared thicker and more compact than the normal PDLSC sheets, demonstrated more layers of cells (3-7 layers), secreted richer extracellular matrix (ECM), showed varying degrees of increases in mRNA expression of periodontal tissue-specific genes (COL I, POSTN), calcification-related genes (RUNX2, OPN, OCN) and a cementum tissue-specific gene (CAP), and possessed a better mineralization ability in terms of osteogenic differentiation in vitro. These EMD-enhanced cell sheets may represent a potential option for stem-cell therapy for PDL regeneration.
authors: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors: Zhongshan
authors: Z
authors: Wang
authors: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors: Zhihong
authors: Z
authors: Feng
authors: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors: Guofeng
authors: G
authors: Wu
authors: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors: Shizhu
authors: S
authors: Bai
authors: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
authors: Yan
authors: Y
authors: Dong
authors: State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. Electronic address: zhaoymdentist@163.com.
authors: Yimin
authors: Y
authors: Zhao
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000328:Adult; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013552:Swine; D014815:Vitamins / Q000494:pharmacology; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D003746:Dental Enamel Proteins; C512835:POSTN protein, human; D014815:Vitamins; C109069:enamel matrix proteins; D001205:Ascorbic Acid
keywords: Differentiation; EMD; Periodontal ligament; Proliferation; Stem cells