title: Antitumor effects of galectin-3 inhibition in human renal carcinoma cells.
abstract: Galectins are thought to be prognosticators for survival in renal cell cancer. However, the biological activity of galectin-3 (Gal-3) in renal carcinoma cells is still debated. In this study, immunohistochemical staining confirmed a high expression of Gal-3 in tumor tissue from renal cell carcinoma. Critically, Gal-3 expression was related to tumor cell differentiation. Consistent with Gal-3 expression in renal cell cancer, strong expression of Gal-3 was also observed in several renal tumor cell lines but not in normal renal cells. A Gal-3 high-expression cell line Caki-1 was chosen to study the biological activity of Gal-3. Using short hairpin RNA method, Gal-3 expression in Caki-1 cells was knocked down. We evidenced that Gal-3 knockdown inhibited cell proliferation and invasion, induced Caspase-3-dependent apoptosis and arrested cell cycle at G1 phase. Mechanically, Cyclin D1 expression decreased, but p27 increased after Gal-3 knockdown. Taken together, these results suggest that Gal-3 is related to the development of renal cell cancer and could serve as a target to therapy renal cell cancer.
authors: Cancer Hospital of Harbin Medical University, Harbin, 150000, China.
authors: Yangyang
authors: Y
authors: Xu
authors: Cancer Hospital of Harbin Medical University, Harbin, 150000, China.
authors: Changfu
authors: C
authors: Li
authors: The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.
authors: Jiahang
authors: J
authors: Sun
authors: The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.
authors: Jingshu
authors: J
authors: Li
authors: Cancer Hospital of Harbin Medical University, Harbin, 150000, China xin_gu123@sina.com.
authors: Xin
authors: X
authors: Gu
authors: The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150081, China xuwanhai_789@outlook.com.
authors: Wanhai
authors: W
authors: Xu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D019938:Cyclin D1 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics; D037502:Galectin 3 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D037502:Galectin 3; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D053148:Caspase 3
keywords: Galectin 3; cell cycle; p27; renal carcinoma cells