title: Membrane tubule formation by banana-shaped proteins with or without transient network structure.
abstract: In living cells, membrane morphology is regulated by various proteins. Many membrane reshaping proteins contain a Bin/Amphiphysin/Rvs (BAR) domain, which consists of a banana-shaped rod. The BAR domain bends the biomembrane along the rod axis and the features of this anisotropic bending have recently been studied. Here, we report on the role of the BAR protein rods in inducing membrane tubulation, using large-scale coarse-grained simulations. We reveal that a small spontaneous side curvature perpendicular to the rod can drastically alter the tubulation dynamics at high protein density, whereas no significant difference is obtained at low density. A percolated network is intermediately formed depending on the side curvature. This network suppresses tubule protrusion, leading to the slow formation of fewer tubules. Thus, the side curvature, which is generated by protein-protein and membrane-protein interactions, plays a significant role in tubulation dynamics. We also find that positive surface tensions and the vesicle membrane curvature can stabilize this network structure by suppressing the tubulation.
authors: Institute for Solid State Physics, University of Tokyo, Kashiwa, Chiba 277-8581, Japan.
authors: Hiroshi
authors: H
authors: Noguchi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry*; D003598:Cytoskeletal Proteins / Q000737:chemistry*; D050556:Fatty Acid-Binding Proteins; D006801:Humans; D008560:Membrane Fluidity; D008869:Microtubule-Associated Proteins / Q000737:chemistry*; D015778:Minor Histocompatibility Antigens / Q000737:chemistry*; D008954:Models, Biological; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C433770:FNBP1 protein, human; D050556:Fatty Acid-Binding Proteins; D008869:Microtubule-Associated Proteins; D015778:Minor Histocompatibility Antigens; C120714:PACSIN2 protein, human; C486382:PSTPIP1 protein, human; C406285:TRIP10 protein, human