title: Vocal Fold Augmentation with Beta Glucan Hydrogel Cross-Linked by γ Irradiation for Enhanced Duration of Effect: In Vivo Animal Study.
abstract: This study explored a novel strategy to restore the vocal gap by using cross-linked β-glucan hydrogel by γ-irradiation. An aqueous solution of 5 wt% β-glucan was prepared and cross-linked using (60)Co γ irradiation. Ten nude mice were injected with 0.8 mL of irradiated β-glucan on the left back and the same volume of nonirradiated β-glucan on the right back for comparison. The mice were sacrificed at 1 and 2 weeks after injection and histological evaluations were performed. Irradiated β-glucan demonstrated a significantly larger volume than nonirradiated β-glucan in the back of nude mice with less inflammatory reaction. After unilateral recurrent laryngeal nerve section in New Zealand White rabbits, irradiated and nonirradiated β-glucan were injected into paralyzed vocal folds. Irradiated β-glucan remained at the paralyzed vocal fold without definite inflammatory signs on endoscopy. High-speed recordings of vocal fold vibration showed decreased vocal gap in irradiated group compared to nonirradiated group. Histologically, the laryngeal epithelium and lamina propria remained intact, without inflammatory cell infiltration. Our newly developed injection material, irradiated β-glucan, showed excellent biocompatibility and remained longer than nonirradiated β-glucan in vivo, suggesting irradiated hydrogels as a new therapeutic approach that may be useful for the long-term treatment of vocal fold palsy.
authors: Research Division for Industry and Environment, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup 580-185, Republic of Korea.
authors: Youn-Mook
authors: YM
authors: Lim
authors: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, 101 Daehak-ro, Seoul 110-744, Republic of Korea.
authors: Bo Hae
authors: BH
authors: Kim
authors: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, 814 Siksa Dong, Goyang 410-773, Republic of Korea.
authors: Hee-Bok
authors: HB
authors: Kim
authors: QueGen Biotech, Jeongwang 1-dong, Siheung-si 429-931, Republic of Korea.
authors: EunJi
authors: E
authors: Park
authors: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, 814 Siksa Dong, Goyang 410-773, Republic of Korea.
authors: Seok-Won
authors: SW
authors: Park
authors: Research Division for Industry and Environment, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup 580-185, Republic of Korea.
authors: Jong-Seok
authors: JS
authors: Park
authors: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, 814 Siksa Dong, Goyang 410-773, Republic of Korea.
authors: Se In
authors: SI
authors: Choi
authors: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, 101 Daehak-ro, Seoul 110-744, Republic of Korea; Department of Otorhinolaryngology, College of Medicine, Seoul National University, 101 Daehak-ro, Seoul 110-744, Republic of Korea.
authors: Tack-Kyun
authors: TK
authors: Kwon
authors: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, 101 Daehak-ro, Seoul 110-744, Republic of Korea; Cancer Research Institute, 101 Daehak-ro, Seoul 110-744, Republic of Korea; Seoul National University Biomedical Research Institute, 101 Daehak-ro, Seoul 110-744, Republic of Korea.
authors: Seong Keun
authors: SK
authors: Kwon
mesh_terms: D000818:Animals; D005720:Gamma Rays; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011817:Rabbits; D014826:Vocal Cord Paralysis / Q000628:therapy*; D014827:Vocal Cords; D047071:beta-Glucans / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020100:Hydrogels; D047071:beta-Glucans