title: Assessment of biochemical parameters and characterization ofTNFα -308G/A andPTPN22+1858C/T gene polymorphisms in the risk of obesity in adolescents.
abstract: Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα -308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol-chloroform method, and TNFα -308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA-IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA-IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα -308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed.
authors: Research Department, Saltillo Unit Faculty of Medicine, Autonomous University of Coahuila, Saltillo, Coahuila CP 2500, Mexico.
authors: Mauricio Andrés
authors: MA
authors: Salinas-Santander
authors: Department of Basic Sciences, Division of Health Sciences, University of Monterrey, San Pedro Garza García, Nuevo León CP 66238, Mexico.
authors: Rafael Baltazar
authors: RB
authors: León-Cachón
authors: Research Department, Saltillo Unit Faculty of Medicine, Autonomous University of Coahuila, Saltillo, Coahuila CP 2500, Mexico.
authors: Ana Cecilia
authors: AC
authors: Cepeda-Nieto
authors: Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Autonomous University of Nuevo León, Monterrey, Nuevo León CP 64460, Mexico.
authors: Celia Nohemí
authors: CN
authors: Sánchez-Domínguez
authors: School of Chemical Sciences, Autonomous University of Coahuila, Saltillo, Coahuila CP 25280, Mexico.
authors: María Antonia
authors: MA
authors: González-Zavala
authors: Department of Genetics, School of Medicine, Autonomous University of Nuevo León, Monterrey, Nuevo León CP 64460, Mexico.
authors: Hugo Leonid
authors: HL
authors: Gallardo-Blanco
authors: Research Department, Saltillo Unit Faculty of Medicine, Autonomous University of Coahuila, Saltillo, Coahuila CP 2500, Mexico.
authors: Sandra Cecilia
authors: SC
authors: Esparza-González
authors: Research Department, Saltillo Unit Faculty of Medicine, Autonomous University of Coahuila, Saltillo, Coahuila CP 2500, Mexico.
authors: Miguel Ángel
authors: MÁ
authors: González-Madrazo
keywords: Mexican population; PCR-RFLP; PTPN22 +1858C/T; TNFα −308G/A; autoimmune diseases; inflammatory; obesity