title: Microsatellite analysis of genotype distribution patterns of Candida albicans vulvovaginal candidiasis in Nanjing, China and its association with pregnancy, age and clinical presentation.
abstract: To characterize the genotype distribution pattern of Candida albicans associated with vulvovaginal candidiasis (VVC) in Nanjing, China by microsatellite genotyping.
  
  
  
  A questionnaire was completed by each patient diagnosed with VVC. A total of 208 independent C. albicans was isolated from 208 patients. Microsatellite genotyping characterized the genotype distribution by analysis of the CAI locus marker.
  
  
  
  PCR of CAI fragments showed the three major genotypes contained 30:45, 21:21 and 32:46 alleles among the 51 genotypes detected, accounting for 29.3, 13.0 and 12.0 % of 208 clinical isolates. Genotype distributions had a similar pattern among different clinical presentations (P = 0.219). In both groups of the (21-30) and (31-40) years, 30:45 was the most frequent genotype allele detected. In the (21-30) year females, 16.5 % of the isolated strains had the genotype 21:21, while the same genotype in the group of (31-40) years was 6.9 %. Genotype distributions were significant difference between the pregnant and non-pregnant women (P < 0.001). 30:45 was detected only one in the 23 pregnant women.
  
  
  
  The results indicated a unique genotype distribution of C. albicans associated with VVC in Nanjing, eastern China and a different distribution pattern was also detected in pregnant women compared to non-pregnant women.
authors: Institute of Dermatology, Chinese Academy of Medical Science and Peaking Union Medical College and Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No 12 Jiangwangmiao St., Nanjing, 210042, China.
authors: Caixia
authors: C
authors: Li
authors: Institute of Dermatology, Chinese Academy of Medical Science and Peaking Union Medical College and Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No 12 Jiangwangmiao St., Nanjing, 210042, China.
authors: Le
authors: L
authors: Wang
authors: Department of Gynecology, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.
authors: Hua
authors: H
authors: Tong
authors: Institute of Dermatology, Chinese Academy of Medical Science and Peaking Union Medical College and Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No 12 Jiangwangmiao St., Nanjing, 210042, China.
authors: Yiping
authors: Y
authors: Ge
authors: Institute of Dermatology, Chinese Academy of Medical Science and Peaking Union Medical College and Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No 12 Jiangwangmiao St., Nanjing, 210042, China.
authors: Huan
authors: H
authors: Mei
authors: Department of Gynecology, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.
authors: Liangyu
authors: L
authors: Chen
authors: Institute of Dermatology, Chinese Academy of Medical Science and Peaking Union Medical College and Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No 12 Jiangwangmiao St., Nanjing, 210042, China.
authors: Guixia
authors: G
authors: Lv
authors: Institute of Dermatology, Chinese Academy of Medical Science and Peaking Union Medical College and Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, No 12 Jiangwangmiao St., Nanjing, 210042, China. liumyco@hotmail.com.
authors: Weida
authors: W
authors: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000483:Alleles; D002176:Candida albicans / Q000235:genetics* / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000175:diagnosis / Q000208:ethnology* / Q000382:microbiology; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D037841:Pregnant People; D014621:Vagina / Q000382:microbiology*
keywords: Candida albicans; Genotype; Microsatellite analysis; Vulvovaginal candidiasis