title: Unique immunomodulatory effect of paeoniflorin on type I and II macrophages activities.
abstract: It has been widely accepted that macrophages are divided into M1 "pro-inflammatory" macrophages and M2 "anti-inflammatory" macrophages and an uncontrolled macrophage polarization plays an important role in the pathogenesis of different diseases. As the main substance of total glucosides of peony, paeoniflorin (PF), has been widely used to treat autoimmune and autoinflammatory diseases for years. Mechanistically, PF has been found to alter activities of many immune cells, which could further reduce inflammation and tissue damage. However, whether and how PF affects macrophages activities in vitro remains unknown. In current study, using M1 and M2 cells generated from mouse bone marrow precursors, we explored the role of PF in regulating M1/M2 cells activity in vitro. The results showed that PF inhibited LPS-induced M1 activity by reducing iNOS expression and NO production via decreasing LPS/NF-κB signaling pathway; whereas, PF enhanced IL-4-provoked M2 function by up-regulating Arg-1 production and activity via increasing IL-4/STAT6 signaling pathway. Our new finding indicates that PF can suppress M1 cells activity and enhance M2 cells function simultaneously, which could help to ameliorate autoimmune and autoinflammatory diseases in clinical treatment.
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Tianhang
authors: T
authors: Zhai
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Rheumatology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, China.
authors: Yue
authors: Y
authors: Sun
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Huidan
authors: H
authors: Li
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Jie
authors: J
authors: Zhang
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Rongfen
authors: R
authors: Huo
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Maternity and Child Health Hospital, Changning, Shanghai, China.
authors: Haichuan
authors: H
authors: Li
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Baihua
authors: B
authors: Shen
authors: Shanghai Institute of Immunology & Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: ninglixiaoxue57@163.com.
authors: Ningli
authors: N
authors: Li
mesh_terms: D000818:Animals; D001119:Arginase; D001327:Autoimmune Diseases / Q000188:drug therapy; D016764:Cell Polarity / Q000187:drug effects; D002478:Cells, Cultured; D005960:Glucosides / Q000494:pharmacology* / Q000627:therapeutic use; D056747:Immunomodulation; D015847:Interleukin-4; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000145:classification / Q000276:immunology*; D008297:Male; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D016328:NF-kappa B; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D008517:Phytotherapy; D050800:STAT6 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects; D006027:Glycosides
chemical_list: D001119:Arginase; D005960:Glucosides; D015847:Interleukin-4; D008070:Lipopolysaccharides; D039821:Monoterpenes; D016328:NF-kappa B; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse; C015423:peoniflorin; D006027:Glycosides; C544448:Arg1 protein, mouse
keywords: Autoimmune and autoinflammatory diseases; M1 macrophage; M2 macrophage; Paeoniflorin