title: Rapid determination of chemical composition and classification of bamboo fractions using visible-near infrared spectroscopy coupled with multivariate data analysis.
abstract: BACKGROUND
  During conversion of bamboo into biofuels and chemicals, it is necessary to efficiently predict the chemical composition and digestibility of biomass. However, traditional methods for determination of lignocellulosic biomass composition are expensive and time consuming. In this work, a novel and fast method for quantitative and qualitative analysis of chemical composition and enzymatic digestibilities of juvenile bamboo and mature bamboo fractions (bamboo green, bamboo timber, bamboo yellow, bamboo node, and bamboo branch) using visible-near infrared spectra was evaluated.
  
  
  RESULTS
  The developed partial least squares models yielded coefficients of determination in calibration of 0.88, 0.94, and 0.96, for cellulose, xylan, and lignin of bamboo fractions in raw spectra, respectively. After visible-near infrared spectra being pretreated, the corresponding coefficients of determination in calibration yielded by the developed partial least squares models are 0.994, 0.990, and 0.996, respectively. The score plots of principal component analysis of mature bamboo, juvenile bamboo, and different fractions of mature bamboo were obviously distinguished in raw spectra. Based on partial least squares discriminant analysis, the classification accuracies of mature bamboo, juvenile bamboo, and different fractions of bamboo (bamboo green, bamboo timber, bamboo yellow, and bamboo branch) all reached 100 %. In addition, high accuracies of evaluation of the enzymatic digestibilities of bamboo fractions after pretreatment with aqueous ammonia were also observed.
  
  
  CONCLUSIONS
  The results showed the potential of visible-near infrared spectroscopy in combination with multivariate analysis in efficiently analyzing the chemical composition and hydrolysabilities of lignocellulosic biomass, such as bamboo fractions.
authors: Research Institute of Forestry New Technology, Chinese Academy of Forestry, 100091 Beijing, China ; Research Institute of Wood Industry, Chinese Academy of Forestry, 100091 Beijing, China.
authors: Zhong
authors: Z
authors: Yang
authors: Research Institute of Wood Industry, Chinese Academy of Forestry, 100091 Beijing, China.
authors: Kang
authors: K
authors: Li
authors: Research Institute of Wood Industry, Chinese Academy of Forestry, 100091 Beijing, China.
authors: Maomao
authors: M
authors: Zhang
authors: College of Forestry, Northwest A&F University, 3 Taicheng Road, 712100 Yangling, China.
authors: Donglin
authors: D
authors: Xin
authors: College of Forestry, Northwest A&F University, 3 Taicheng Road, 712100 Yangling, China.
authors: Junhua
authors: J
authors: Zhang
keywords: Bamboo; Botanical fractions; Multivariate analysis; Qualitative classification; Quantitative analysis