title: Analysis of HIV-1c-Specific CTL Responses with HIV-1 Reservoir Size and Forms.
abstract: Cytotoxic T lymphocytes (CTL) are critical in cellular immune responses; therefore the study of CTL responses is profound in HIV-1 eradication. We aim to dissect the relationship between HIV-1 reservoir size and the magnitude and recognition of viral-specific CTL responses. An IFN-γ ELISpot assay with peptides spanning the HIV-1 clade C consensus sequences were designed to analyze HIV-1c-specific CTL responses. HIV-1 DNA, integrated HIV-1 DNA, and 2-LTR HIV-1 DNA were quantitated by real-time PCR. We observed significant increases in total HIV-1 DNA and integrated HIV-1 DNA after highly active antiretroviral treatment (HAART) compared with naive patients. Total HIV-1 DNA had a significant negative correlation with HIV-1c-specific CTL response magnitude. Baseline CD4(+) T lymphocyte counts and antiretroviral treatment affected the size of the HIV-1 reservoirs. Taken together, HIV-1-specific CTL responses correlated with the size of HIV-1 reservoir. In addition, HIV-1-specific CTL response against p17 was associated with low integral efficiency of HIV-1, which might be a biomarker to evaluate the efficacy of HAART.
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Wenzhen
authors: W
authors: Kang
authors: 2 AIDS Research Center, MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology , Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China .
authors: Weijun
authors: W
authors: Zhu
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Yuan
authors: Y
authors: Li
authors: 3 Center for Infectious Diseases, Beijing You-An Hospital, Capital Medical University , Beijing, People's Republic of China .
authors: Yanmei
authors: Y
authors: Jiao
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Yan
authors: Y
authors: Zhuang
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Yumei
authors: Y
authors: Xie
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Ke
authors: K
authors: Zhao
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Bianli
authors: B
authors: Dang
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Qingquan
authors: Q
authors: Liu
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Ye
authors: Y
authors: Zhang
authors: 1 Department of Infectious Diseases, Tangdu Hospital Affiliated to the Fourth Military Medical University , Xi'an, People's Republic of China .
authors: Yongtao
authors: Y
authors: Sun
mesh_terms: D000328:Adult; D019380:Anti-HIV Agents / Q000627:therapeutic use*; D023241:Antiretroviral Therapy, Highly Active; D015415:Biomarkers; D018791:CD4 Lymphocyte Count; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D015415:Biomarkers; D004279:DNA, Viral