title: MALDI MSI analysis of lipid changes in living skin equivalents in response to emollient creams containing palmitoylethanolamide.
abstract: Mass spectrometry imaging (MSI) is a powerful tool for the study of intact tissue sections. The use of matrix-assisted laser desorption/ionisation (MALDI) MSI for the study of the distribution and effect of emollient treatment on sections of reconstructed living skin equivalents during their development and maturation is described. Living skin equivalent (LSE) samples were obtained at 14days development, re-suspended in maintenance medium and incubated for 24h after delivery. The medium was changed, the LSE treated with either Physiogel A.I.® or Oilatum Junior® emollients and then re-incubated and samples taken at 4, 6 and 24h time points. Mass spectra and mass spectral images were recorded from 12μm sections of the LSE taken at each time point for comparison using MALDI mass spectrometry (MS). It was possible to detect ions characteristic of each emollient in the LSE. In addition a number of lipid species previously reported as being significant in the maturation of the LSE were observable. At the 24h time point, the images revealed what appeared to be differences in the organisation of the skin cells observed across the Physiogel A.I.® treatment group tissue sections when directly compared to the untreated tissue group.
authors: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom. Electronic address: christopher-mitchell@live.co.uk.
authors: Christopher A
authors: CA
authors: Mitchell
authors: Stiefel A GSK Company, GlaxoSmithKline, Stockley Park West, Uxbridge, Middlesex UB1 1BT, United Kingdom. Electronic address: michael.x.donaldson@stiefel.com.
authors: Michael
authors: M
authors: Donaldson
authors: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom. Electronic address: s.francese@shu.ac.uk.
authors: Simona
authors: S
authors: Francese
authors: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom. Electronic address: m.r.clench@shu.ac.uk.
authors: Malcolm R
authors: MR
authors: Clench
mesh_terms: D000577:Amides; D004643:Emollients / Q000009:adverse effects / Q000494:pharmacology; D004983:Ethanolamines / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D010169:Palmitic Acids / Q000737:chemistry* / Q000378:metabolism; D012867:Skin / Q000737:chemistry* / Q000187:drug effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000577:Amides; D004643:Emollients; D004983:Ethanolamines; D008055:Lipids; D010169:Palmitic Acids; C005958:palmidrol
keywords: Emollients; Lipids; Living skin equivalents; MALDI; MALDI MSI; MS imaging; Skin