title: Hepatoprotective Activity of Water Extracts from Chaga Medicinal Mushroom, Inonotus obliquus (Higher Basidiomycetes) Against Tert-Butyl Hydroperoxide-Induced Oxidative Liver Injury in Primary Cultured Rat Hepatocytes.
abstract: We examined the hepatoprotective activity of Inonotus obliquus water extract (IO-W) against tert-butyl hydroperoxide (t-BHP)-induced oxidative liver injury in the primary cultured rat hepatocyte. The 50% radical scavenging concentrations (SC50s) of IO-W for radical-scavenging activity against 2,2'-azino-bis-(3-ethylbenzothi- azoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) were 5.19 mg/mL and 0.39 mg/mL, respectively. IO-W pretreatment to the primary cultured hepatocytes significantly (p<0.05) protected the cells from t-BHP-induced cytotoxic injury even at a low concentration of IO-W (10 µg/mL). The cellular leakage of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), as well as malondialdehyde (MDA) formation caused by t-BHP were significantly (p<0.05) suppressed by IO-W pretreatment (>100 µg/ mL). In conclusion, this study demonstrates that IO-W exhibited hepatoprotective activity against t-BHP-induced oxidative liver injury in the primary cultured hepatocyte probably via its abilities of quenching free radicals, inhibiting the leakage of ALT, AST, and LDH, and decreasing MDA formation.
authors: Department of Public Health Science, Graduate School, Korea University, Seoul, Republic of Korea.
authors: Ki Bae
authors: KB
authors: Hong
authors: Department of Hotel Culinary Arts and Nutrition, Kaya University, Gyeongnam, Republic of Korea.
authors: Dong Ouk
authors: DO
authors: Noh
authors: Department of Food and Nutrition, Korea University, Seoul, Republic of Korea.
authors: Yooheon
authors: Y
authors: Park
authors: Department of Food and Nutrition, Korea University, Seoul, Republic of Korea.
authors: Hyung Joo
authors: HJ
authors: Suh
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D000410:Alanine Transaminase; D005978:Glutathione