title: Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages.
abstract: Hepatic ischemia-reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia-reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia-reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondrial function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia-reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic IR injury.
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.|The School of Life Sciences and Technology, Tongji University, Shanghai, China.
authors: Weisheng
authors: W
authors: Zheng
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Hewei
authors: H
authors: Men
authors: Department of Gastroenterology, Tianjin First Central Hospital, Tianjin, China.|Tianjin Medical University, Tianjin, China.
authors: Jing
authors: J
authors: Li
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Yu
authors: Y
authors: Xing
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Bin
authors: B
authors: Wu
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Zhenglu
authors: Z
authors: Wang
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Junjie
authors: J
authors: Li
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Dahong
authors: D
authors: Teng
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Yuan
authors: Y
authors: Shi
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Jiang
authors: J
authors: Li
authors: Department of Forensic Medicine, College of Basic Medicine, Chongqing Medical University, Chongqing, China.
authors: Pu
authors: P
authors: Jiang
authors: Department of Transplant Surgery, Tianjin First Central Hospital, Tianjin, China.
authors: Jinzhen
authors: J
authors: Cai
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D007511:Ischemia / Q000235:genetics / Q000473:pathology; D008099:Liver / Q000098:blood supply / Q000293:injuries* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D015427:Reperfusion Injury / Q000235:genetics* / Q000473:pathology
chemical_list: D035683:MicroRNAs