title: Methods for SWATH™: Data Independent Acquisition on TripleTOF Mass Spectrometers.
abstract: Data independent acquisition (DIA also termed SWATH) is an emerging technology in the field of mass spectrometry based proteomics. Although the concept of DIA has been around for over a decade, the recent advancements, in particular the speed of acquisition, of mass analyzers have pushed the technique into the spotlight and allowed for high-quality DIA data to be routinely acquired by proteomics labs. In this chapter we will discuss the protocols used for DIA acquisition using the Sciex TripleTOF mass spectrometers and data analysis using the Sciex processing software.
authors: Advanced Clinical Biosystems Research Institute, The Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA. ronald.holewinski@cshs.org.
authors: Ronald J
authors: RJ
authors: Holewinski
authors: Advanced Clinical Biosystems Research Institute, The Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors: Sarah J
authors: SJ
authors: Parker
authors: Advanced Clinical Biosystems Research Institute, The Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors: Andrea D
authors: AD
authors: Matlock
authors: Advanced Clinical Biosystems Research Institute, The Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors: Vidya
authors: V
authors: Venkatraman
authors: Advanced Clinical Biosystems Research Institute, The Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors: Jennifer E
authors: JE
authors: Van Eyk
mesh_terms: D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012984:Software
keywords: Data independent acquisition (DIA); Mass spectrometry; Quantitative proteomics; SWATH; Spectral ion library