title: Molecular Detection of Human Cytomegalovirus (HCMV) Among Infants with Congenital Anomalies in Khartoum State, Sudan.
abstract: Human Cytomegalovirus (HCMV) infection still represents the most common potentially serious viral complication in humans and is a major cause of congenital anomalies in infants. This study is aimed to detect HCMV in infants with congenital anomalies. Study subjects consisted of infants born with neural tube defect, hydrocephalus and microcephaly. Fifty serum specimens (20 males, 30 females) were collected from different hospitals in Khartoum State. The sera were investigated for cytomegalovirus specific immunoglobin M (IgM) antibodies using enzyme-linked immunosorbent assay (ELISA), and for Cytomegalovirus DNA using polymerase chain reaction (PCR). Out of the 50 sera tested, one patient's (2%) sample showed HCMV IgM, but with no detectable DNA, other 4(8.2 %) sera were positive for HCMV DNA but with no detectable IgM. Various diagnostic techniques should be considered to evaluate HCMV disease and routine screening for HCMV should be introduced for pregnant women in this setting. It is vital to initiate further research work with many samples from different area to assess prevalence and characterize HCMV and evaluate its maternal health implications.
authors: Department of Microbiology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
authors: Maha G
authors: MG
authors: Ebrahim
authors: Department of Microbiology, Faculty of Medical Laboratory Sciences, National University, Sudan.
authors: Aisha S
authors: AS
authors: Ali
authors: Departmemt of Virology, Central Laboratory, Ministry of Science and Communication, Khartoum, Sudan.
authors: Mohamed O
authors: MO
authors: Mustafa
authors: Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University Science and Technology, Sudan.
authors: Dalal F
authors: DF
authors: Musa
authors: Departmemt of Virology, Central Laboratory, Ministry of Science and Communication, Khartoum, Sudan.
authors: Abdel Rahim M
authors: AR
authors: El Hussein
authors: Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan.
authors: Isam M
authors: IM
authors: Elkhidir
authors: Departmemt of Virology, Central Laboratory, Ministry of Science and Communication, Khartoum, Sudan.
authors: Khalid A
authors: KA
authors: Enan
keywords: Congenital anomalies; ELISA; HCMV; PCR; Sudan