title: Development of Nested PCR-Based Specific Markers for Detection of Peach Rosette Mosaic Virus in Plant Quarantine.
abstract: The Peach rosette mosaic virus (PRMV) is a plant pathogen of the genus Nepovirus, and has been designated as a controlled quarantine virus in Korea. In this study, a specific reverse transcription (RT)-PCR marker set, nested PCR marker set, and modified-plasmid positive control were developed to promptly and accurately diagnose PRMV at plant-quarantine sites. The final selected PRMV-specific RT-PCR marker was PRMV-N10/C70 (967 bp), and the nested PCR product of 419 bp was finally amplified. The modified-plasmid positive control, in which the SalI restriction-enzyme region (GTCGAC) was inserted, verified PRMV contamination in a comparison with the control, enabling a more accurate diagnosis. It is expected that the developed method will continuously contribute to the plant-quarantine process in Korea.
authors: Environmental Infrastructure Research Department, National Institute of Environmental Research, Incheon, 440-170 Korea.
authors: S
authors: 0000-0001-7552-7681
authors: S
authors: Lee
authors: Environmental Infrastructure Research Department, National Institute of Environmental Research, Incheon, 440-170 Korea.
authors: C S
authors: CS
authors: Kim
authors: Incheon International Airport Regional Office, Animal and Plant Quarantine Agency, Incheon, 440-044 Korea.
authors: Y G
authors: YG
authors: Shin
authors: Department of Chemistry, College of Natural Science, Dankook University, Cheonan, 330-714 Korea.
authors: J H
authors: JH
authors: Kim
authors: Yeongsan River Environment Research Center, National Institute of Environmental Research, Gwangju, 500-480 Korea.
authors: Y S
authors: YS
authors: Kim
authors: Environmental Health Research Department, National Institute of Environmental Research, Incheon, 440-170 Korea.
authors: W H
authors: WH
authors: Jheong
keywords: Nested PCR; PRMV; Peach rosette mosaic virus; Quarantine; RT-PCR