title: Gastric microbiota and predicted gene functions are altered after subtotal gastrectomy in patients with gastric cancer.
abstract: Subtotal gastrectomy (i.e., partial removal of the stomach), a surgical treatment for early-stage distal gastric cancer, is usually accompanied by highly selective vagotomy and Billroth II reconstruction, leading to dramatic changes in the gastric environment. Based on accumulating evidence of a strong link between human gut microbiota and host health, a 2-year follow-up study was conducted to characterize the effects of subtotal gastrectomy. Gastric microbiota and predicted gene functions inferred from 16S rRNA gene sequencing were analyzed before and after surgery. The results demonstrated that gastric microbiota is significantly more diverse after surgery. Ralstonia and Helicobacter were the top two genera of discriminant abundance in the cancerous stomach before surgery, while Streptococcus and Prevotella were the two most abundant genera after tumor excision. Furthermore, N-nitrosation genes were prevalent before surgery, whereas bile salt hydrolase, NO and N2O reductase were prevalent afterward. To our knowledge, this is the first report to document changes in gastric microbiota before and after surgical treatment of stomach cancer.
authors: Bioinformatics Program, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan.|Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan.|Institute of Biomedical Informatics, National Yang-Ming University, Taipei 11221, Taiwan.
authors: Ching-Hung
authors: CH
authors: Tseng
authors: School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan.|Institute of Population Health Sciences, National Health Research Institutes, Miaoli 35053, Taiwan.
authors: Jaw-Town
authors: JT
authors: Lin
authors: Division of Gastroenterology, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
authors: Hsiu J
authors: HJ
authors: Ho
authors: Division of Gastroenterology, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
authors: Zi-Lun
authors: ZL
authors: Lai
authors: Department of Public Health, China Medical University, Taichung 40402, Taiwan.
authors: Chang-Bi
authors: CB
authors: Wang
authors: Bioinformatics Program, Taiwan International Graduate Program, Academia Sinica, Taipei 11529, Taiwan.|Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan.
authors: Sen-Lin
authors: SL
authors: Tang
authors: Division of Gastroenterology, Taichung Veterans General Hospital, Taichung 40705, Taiwan.|Department of Public Health, China Medical University, Taichung 40402, Taiwan.|Graduate Institute of Clinical Medical Sciences, China Medical University, Taichung 40402, Taiwan.|Faculty of Medicine and Graduate Institute of Clinical Medicine, National Yang-Ming University, Taipei 11221, Taiwan.|National Institute of Cancer Research, National Health Research Institutes, Miaoli 35053, Taiwan.|Department of Life Sciences and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, Taichung 40227, Taiwan.
authors: Chun-Ying
authors: CY
authors: Wu
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D005500:Follow-Up Studies; D005743:Gastrectomy; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D064307:Microbiota; D010088:Oxidoreductases / Q000378:metabolism; D016133:Polymerase Chain Reaction; D018720:Prevotella / Q000235:genetics / Q000302:isolation & purification; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D043367:Ralstonia / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D013270:Stomach / Q000382:microbiology*; D013274:Stomach Neoplasms / Q000473:pathology / Q000601:surgery*; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C034747:nitric-oxide reductase; D000581:Amidohydrolases; C023154:choloylglycine hydrolase