title: B Cells Negatively Regulate the Establishment of CD49b(+)T-bet(+) Resting Memory T Helper Cells in the Bone Marrow.
abstract: During an immune reaction, some antigen-experienced CD4 T cells relocate from secondary lymphoid organs (SLOs) to the bone marrow (BM) in a CD49b-dependent manner and reside and rest there as professional memory CD4 T cells. However, it remains unclear how the precursors of BM memory CD4 T cells are generated in the SLOs. While several studies have so far shown that B cell depletion reduces the persistence of memory CD4 T cells in the spleen, we here show that B cell depletion enhances the establishment of memory CD4 T cells in the BM and that B cell transfer conversely suppresses it. Interestingly, the number of antigen-experienced CD4 T cells in the BM synchronizes the number of CD49b(+)T-bet(+) antigen-experienced CD4 T cells in the spleen. CD49b(+)T-bet(+) antigen-experienced CD4 T cells preferentially localize in the red pulp area of the spleen and the BM in a T-bet-independent manner. We suggest that B cells negatively control the generation of CD49b(+)T-bet(+) precursors of resting memory CD4 T cells in the spleen and may play a role in bifurcation of activated effector and resting memory CD4 T cell lineages.
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute , Berlin , Germany.
authors: Shintaro
authors: S
authors: Hojyo
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute , Berlin , Germany.
authors: Jana
authors: J
authors: Sarkander
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute , Berlin , Germany.
authors: Christian
authors: C
authors: Männe
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute , Berlin , Germany.
authors: Mathias
authors: M
authors: Mursell
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute , Berlin , Germany.
authors: Asami
authors: A
authors: Hanazawa
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute, Berlin, Germany; Experimental Immunology and Osteoarthritis Research, Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors: David
authors: D
authors: Zimmel
authors: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD , USA.
authors: Jinfang
authors: J
authors: Zhu
authors: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD , USA.
authors: William E
authors: WE
authors: Paul
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute, Berlin, Germany; INSERM U1151-CNRS UMR 8253, Institut Necker-Enfants Malades, Paris, France; Faculté de Médecine, Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpital Necker Enfants Malades, Paris, France.
authors: Simon
authors: S
authors: Fillatreau
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute, Berlin, Germany; Experimental Immunology and Osteoarthritis Research, Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors: Max
authors: M
authors: Löhning
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute , Berlin , Germany.
authors: Andreas
authors: A
authors: Radbruch
authors: Deutsches Rheuma-Forschungszentrum Berlin, Leibniz Institute , Berlin , Germany.
authors: Koji
authors: K
authors: Tokoyoda
keywords: B cells; CD4 T helper cells; T-bet; bone marrow; resting memory