title: Immune Dysfunction Associated with Abnormal Bone Marrow-Derived Mesenchymal Stroma Cells in Senescence Accelerated Mice.
abstract: Senescence accelerated mice (SAM) are a group of mice that show aging-related diseases, and SAM prone 10 (SAMP10) show spontaneous brain atrophy and defects in learning and memory. Our previous report showed that the thymus and the percentage of T lymphocytes are abnormal in the SAMP10, but it was unclear whether the bone marrow-derived mesenchymal stroma cells (BMMSCs) were abnormal, and whether they played an important role in regenerative medicine. We thus compared BMMSCs from SAMP10 and their control, SAM-resistant (SAMR1), in terms of cell cycle, oxidative stress, and the expression of PI3K and mitogen-activated protein kinase (MAPK). Our cell cycle analysis showed that cell cycle arrest occurred in the G0/G1 phase in the SAMP10. We also found increased reactive oxygen stress and decreased PI3K and MAPK on the BMMSCs. These results suggested the BMMSCs were abnormal in SAMP10, and that this might be related to the immune system dysfunction in these mice.
authors: Department of Stem Cell Disorders, Kansai Medical University, Hirakata City, Osaka 573-1010, Japan. liming@hirakata.kmu.ac.jp.
authors: Ming
authors: M
authors: Li
authors: Department of Cardiac Surgery, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100029, China. guokequan@hotmail.com.
authors: Kequan
authors: K
authors: Guo
authors: Department of Stem Cell Disorders, Kansai Medical University, Hirakata City, Osaka 573-1010, Japan. adachiya250@gmail.com.|Division of Surgical Pathology, Toyooka Hospital, Hyogo 668-8501, Japan. adachiya250@gmail.com.
authors: Yasushi
authors: Y
authors: Adachi
authors: Department of Stem Cell Disorders, Kansai Medical University, Hirakata City, Osaka 573-1010, Japan. ikehara@hirakata.kmu.ac.jp.
authors: Susumu
authors: S
authors: Ikehara
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology; D000375:Aging / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018384:Oxidative Stress; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism
chemical_list: D016207:Cytokines; D019869:Phosphatidylinositol 3-Kinases
keywords: MAPK; PI3K; SAMP10; bone marrow-derived MSCs; cell cycle