title: Porcine aminopeptidase N binds to F4+ enterotoxigenic Escherichia coli fimbriae.
abstract: F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+) E. coli to intestinal epithelial cells.
authors: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China. pengpengxia@hotmail.com.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. pengpengxia@hotmail.com.
authors: Pengpeng
authors: P
authors: Xia
authors: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China. 296730702@qq.com.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. 296730702@qq.com.
authors: Yiting
authors: Y
authors: Wang
authors: College of Animal Medicine, Nanjing Agriculture University, Nanjing, 210095, China. yzcongruizhu@outlook.com.
authors: Congrui
authors: C
authors: Zhu
authors: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China. 674652493@qq.com.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. 674652493@qq.com.
authors: Yajie
authors: Y
authors: Zou
authors: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China. yang_ying510@163.com.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. yang_ying510@163.com.
authors: Ying
authors: Y
authors: Yang
authors: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China. Lizza0711@sina.com.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. Lizza0711@sina.com.
authors: Wei
authors: W
authors: Liu
authors: College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. hardwidg@gmail.com.
authors: Philip R
authors: PR
authors: Hardwidge
authors: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China. yzgqzhu@yzu.edu.cn.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. yzgqzhu@yzu.edu.cn.
authors: Guoqiang
authors: G
authors: Zhu
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D018826:CD13 Antigens / Q000235:genetics / Q000378:metabolism*; D054307:Enterotoxigenic Escherichia coli / Q000502:physiology*; D004847:Epithelial Cells / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000502:physiology*; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology
chemical_list: D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; C494388:FaeG protein, E coli; D018826:CD13 Antigens