title: Epidermal Growth Factor Receptor-Specific Nanoprobe Biodistribution in Mouse Models.
abstract: Nanotechnology offers a targeted approach to both imaging and treatment of cancer, the leading cause of death worldwide. Previous studies have found that nanoparticles with a wide variety of coatings initiate an immune response leading to sequestration in the liver and spleen. In an effort to find a nanoparticle platform which does not elicit an immune response, we created 43 nm and 44 nm of gold and silver nanoparticles coated with biomolecules normally produced by the body, α-lipoic acid and the epidermal growth factor (EGF), and have used mass spectroscopy to determine their biodistribution in mouse models, 24 h after tail vein injection. Relative to controls, mouse EGF (mEGF)-coated silver and gold nanoprobes are found at background levels in all organs including the liver and spleen. The lack of sequestration of mEGF-coated nanoprobes in the liver and spleen and the corresponding uptake of control nanoprobes at elevated levels in these organs suggest that the former are not recognized by the immune system. Further studies of cytokine and interleukin levels in the blood are required to confirm avoidance of an immune response.
authors: Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.
authors: Christopher L D
authors: CL
authors: Lee
authors: Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.
authors: Samia B
authors: SB
authors: Fashir
authors: Laboratório de Nanossensores, Instituto de Pesquisa e Desenvolvimento-IP&D, Universidade do Vale do Paraíba, São Paulo, 12.244-000, Brasil.
authors: Maiara L
authors: ML
authors: Castilho
authors: Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.
authors: Michael A
authors: MA
authors: Hupman
authors: Laboratório de Nanossensores, Instituto de Pesquisa e Desenvolvimento-IP&D, Universidade do Vale do Paraíba, São Paulo, 12.244-000, Brasil.
authors: Leandro J
authors: LJ
authors: Raniero
authors: Department of Surgery, Dalhousie University, Halifax, Nova Scotia, B3H 2Y9, Canada; Department of Pathology, Dalhousie University, Halifax, Nova Scotia, B3H 2Y9, Canada; Department of Microbiology & Immunology, Dalhousie University, Halifax, Nova Scotia, B3H 2Y9, Canada.
authors: Ian
authors: I
authors: Alwayn
authors: Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada. Electronic address: Kevin.Hewitt@Dal.ca.
authors: Kevin C
authors: KC
authors: Hewitt
mesh_terms: D000818:Animals; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000493:pharmacokinetics; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D006046:Gold / Q000737:chemistry / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D015335:Molecular Probes / Q000737:chemistry / Q000493:pharmacokinetics*; D050997:Nanomedicine / Q000379:methods*; D012834:Silver / Q000737:chemistry / Q000493:pharmacokinetics; D013499:Surface Properties; D008063:Thioctic Acid / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D015335:Molecular Probes; D012834:Silver; D004815:Epidermal Growth Factor; D008063:Thioctic Acid; D006046:Gold; D066246:ErbB Receptors
keywords: biodistribution; epidermal growth factor; gold; mice; nanoparticles; pharmacokinetics; silver; α-lipoic acid