title: Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR.
abstract: Methylation of cytosines plays an important role in epigenetic regulation of gene expression. Several methods exist to determine the methylation status of DNA. Here, we describe a rapid and cost-effective method called Chop-qPCR to determine dynamic changes in the DNA methylation patterns, as they occur for instance in response to environmental stresses.
authors: Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany.|Chemical Genomics Centre (CGC) of the Max Planck Society, 44227, Dortmund, Germany.|MPI for Developmental Biology, 72076, Tübingen, Germany.
authors: Stephanie
authors: S
authors: Rausch
authors: Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany. sascha.laubinger@zmbp.uni-tuebingen.de.|Chemical Genomics Centre (CGC) of the Max Planck Society, 44227, Dortmund, Germany. sascha.laubinger@zmbp.uni-tuebingen.de.|MPI for Developmental Biology, 72076, Tübingen, Germany. sascha.laubinger@zmbp.uni-tuebingen.de.
authors: Sascha
authors: S
authors: Laubinger
mesh_terms: D019175:DNA Methylation / Q000187:drug effects* / Q000235:genetics*; D044127:Epigenesis, Genetic / Q000187:drug effects / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology*
chemical_list: D020156:Salicylic Acid
keywords: Arabidopsis thaliana; Biotic stress; Chop-qPCR; DNA methylation; Epigenetics; McrBC; Methylation-dependent restriction enzyme