title: Expression of the voltage-sensing phosphatase gene in the chick embryonic tissues and in the adult cerebellum.
abstract: Voltage-sensing phosphatase (VSP) consists of a transmembrane voltage sensor domain (VSD) and the cytoplasmic domain with phosphoinositide-phosphatase activities. It operates as the voltage sensor and directly translates membrane potential into phosphoinositide turnover by coupling VSD to the cytoplasmic domain. VSPs are evolutionarily conserved from marine invertebrate up to humans. Recently, we demonstrated that ectopic expression of the chick ortholog of VSP, Gg-VSP, in a fibroblast cell line caused characteristic cell process outgrowths. Co-expression of chick PTEN suppressed such morphological change, suggesting that VSP regulates cell shape by increasing PI(3,4)P2. However, the in vivo function of Gg-VSP remains unclear. Here, we showed that in chick embryos Gg-VSP is expressed in the stomach, mesonephros, pharyngeal arch, limb bud, somites, floor plate of neural tube, and notochord. In addition, both Gg-VSP transcripts and the protein were found in the cerebellar Purkinje neurons. These findings provide an insight into the physiological functions of VSP.
authors: Faculty of Pharmaceutical Sciences; Department of Life and Health Sciences ; Teikyo University ; Tokyo, Japan.
authors: Shinji
authors: S
authors: Yamaguchi
authors: Faculty of Pharmaceutical Sciences; Department of Life and Health Sciences ; Teikyo University ; Tokyo, Japan.
authors: Naoya
authors: N
authors: Aoki
authors: Faculty of Pharmaceutical Sciences; Department of Life and Health Sciences ; Teikyo University ; Tokyo, Japan.
authors: Takaaki
authors: T
authors: Kitajima
authors: Laboratory of Integrative Physiology; Graduate School of Medicine; Osaka University; Osaka, Japan; Graduate School of Frontier Bioscience; Osaka University; Osaka, Japan.
authors: Yasushi
authors: Y
authors: Okamura
authors: Faculty of Pharmaceutical Sciences; Department of Life and Health Sciences ; Teikyo University ; Tokyo, Japan.
authors: Koichi J
authors: KJ
authors: Homma
keywords: Gallus gallus domesticus; PTEN; Purkinje neuron; floor plate; mesonephros; notochord; phosphoinositide; somite; voltage-sensing phosphatase