title: Role of the Apolipoprotein E Polymorphisms in the Development of Retinal Vein Occlusion in a Tunisian Population: A Case-Control Study.
abstract: Apolipoprotein E ( APOE) is a member of the apolipoprotein gene family. APOE is polymorphic with 3 main allelic types: ∊2, ∊3, and ∊4. Certain of these alleles have been associated with higher vascular risk. However, the association of APOE genotypes with retinal biomarkers and risk of retinal stroke is less clear. This study evaluated the role of APOE polymorphisms in retinal vein occlusion (RVO). In the present study, 2-point mutations coding amino acid residues 112 and 158 were amplified using the polymerase chain reaction (PCR) from DNA extracted from Tunisian participants. APOE genotypes were determined by multiplex PCR followed by molecular hybridization. Eighty-eight patients (26 women and 62 men) and 100 age- and gender-matched healthy participants were enrolled. The statistical study revealed a higher frequency of the ∊4 allele in patients as compared to controls (27.3% vs 9%) with a significant association of the ∊4 allele with the disease ( P < 10-3, Pa< 10-3, odds ratio [OR] = 3.8, 95% confidence interval [CI] = 2.1-6.8). The frequency of the ∊3 allele was significantly lower in the patients with RVO compared to the controls (60.2% vs 82.5%, respectively; P < 10-3, Pa< 10-3, OR = 0.32, 95% CI = 0.19-0.53). The ∊3 allele seems to be protective against the disease. There was no association between the APO ∊2 allele and RVO. The association of APOE allele and genotype with RVO requires further investigation in different populations.
authors: 1 Laboratoire de Biologie Moléculaire, Service d'Hématologie Hôpital Militaire Principal d'Instruction de Tunis, Montfleury, Tunisie.|2 Faculté des Science de Tunis, Université Tunis el Manar, El Manar, Tunisie.
authors: Meriem
authors: M
authors: Mrad
authors: 1 Laboratoire de Biologie Moléculaire, Service d'Hématologie Hôpital Militaire Principal d'Instruction de Tunis, Montfleury, Tunisie.|3 Académie Militaire Fondouk Jédid, Nabeul, Tunisie.
authors: Najiba
authors: N
authors: Fekih-Mrissa
authors: 4 Service d'Ophtalmologie, Hôpital Militaire Principal d'Instruction de Tunis, Montfleury, Tunisie.|5 Faculté de Médecine de Tunis, Université Tunis el Manar, Tunis, Tunisie.
authors: Cheima
authors: C
authors: Wathek
authors: 1 Laboratoire de Biologie Moléculaire, Service d'Hématologie Hôpital Militaire Principal d'Instruction de Tunis, Montfleury, Tunisie.|2 Faculté des Science de Tunis, Université Tunis el Manar, El Manar, Tunisie.
authors: Aicha
authors: A
authors: Sayeh
authors: 4 Service d'Ophtalmologie, Hôpital Militaire Principal d'Instruction de Tunis, Montfleury, Tunisie.|5 Faculté de Médecine de Tunis, Université Tunis el Manar, Tunis, Tunisie.
authors: Afef
authors: A
authors: Maalej
authors: 4 Service d'Ophtalmologie, Hôpital Militaire Principal d'Instruction de Tunis, Montfleury, Tunisie.|5 Faculté de Médecine de Tunis, Université Tunis el Manar, Tunis, Tunisie.
authors: Riadh
authors: R
authors: Rannen
authors: 1 Laboratoire de Biologie Moléculaire, Service d'Hématologie Hôpital Militaire Principal d'Instruction de Tunis, Montfleury, Tunisie.|6 Faculté de Pharmacie, Université de Monastir, Monastir, Tunisie.
authors: Brahim
authors: B
authors: Nsiri
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001057:Apolipoproteins E / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012170:Retinal Vein Occlusion / Q000453:epidemiology / Q000235:genetics*; D014416:Tunisia / Q000453:epidemiology; D055815:Young Adult
chemical_list: D001057:Apolipoproteins E
keywords: apolipoprotein E; genotypes; multiplex PCR; retinal vein occlusion