title: Apoptosis/Necrosis Induction by Ultraviolet, in ER Positive and ER Negative Breast Cancer Cell Lines.
abstract: BACKGROUND
  Ultraviolet (UV) light exposure has been one of the major inducers of apoptosis. UV exposure has caused pyrimidine dimers and DNA fragmentation which might lead to cell cycle arrest and apoptosis signals activation. UV induced apoptosis has investigated in MDA-MB 468 as an ER negative breast adenocarcinoma and MCF-7 as an ER positive breast cancer cell line. Apoptosis induction rate by UV might be different in these two types of cells due to different biological characteristics of the cell.
  
  
  OBJECTIVE
  In this paper we have evaluated serial dose of UV-B exposure on ER positive and ER negative breast cancer cell lines and its effect on apoptosis or necrosis induction in these cells.
  
  
  METHODS
  MDA-MB468 and MCF-7 cell lines have cultured for 24 hours and UV exposure has carried out at 290 nm at dose of 154 J/m(2) to 18 KJ/m(2) using UV lamp. UV exposed cells have incubated in cell culture condition for 24 or 48 hours following UV exposure and the cells have stained and analyzed by flow cytometry for apoptosis evaluation by Annexin V/PI method.
  
  
  RESULTS
  Apoptosis rate (PI and Annexin V double positive cells) after 24 hours incubation was higher in 24 hours in comparison with 48 hours incubation in both cell lines. The frequency of PI positive MDA-MB 468 cells was higher than PI and Annexin V double positive cells after 48 hours. PI positive MDA-MB 468 cells were significantly higher than MCF-7 cells in 24 hours incubation time.
  
  
  CONCLUSIONS
  The results have shown that MDA-MB 468 cells were more sensitive to UV exposure and DNA fragmentation and necrosis pathway was dominant in these cells.
authors: Cancer Research Center, Department of Immunology, Semnan University of Medical Sciences, Semnan, IR Iran; Student's Research Committee, Semnan University of Medical Sciences, Semnan, IR Iran.
authors: Mahdieh
authors: M
authors: Shokrollahi Barough
authors: Department of Medical Physics, Cancer Research Center, Semnan University of Medical Sciences, Semnan, IR Iran.
authors: Hadi
authors: H
authors: Hasanzadeh
authors: Cancer Research Center, Department of Immunology, Semnan University of Medical Sciences, Semnan, IR Iran; Student's Research Committee, Semnan University of Medical Sciences, Semnan, IR Iran.
authors: Mehdi
authors: M
authors: Barati
authors: Cancer Research Center, Department of Immunology, Semnan University of Medical Sciences, Semnan, IR Iran.
authors: Fatemeh
authors: F
authors: Pak
authors: Cancer Research Center, Department of Immunology, Semnan University of Medical Sciences, Semnan, IR Iran; Immunology Research Center, Iran University of Medical Sciences, Tehran, IR Iran; Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Solna and Karolinska Institute, Immune and Gene Therapy Laboratory, Karolinska University Hospital, Stockholm, Sweden.
authors: Parviz
authors: P
authors: Kokhaei
authors: Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran.
authors: Mostafa
authors: M
authors: Rezaei-Tavirani
keywords: Apoptosis; Cell Line; ER Negative; ER Positive; UV Exposure