title: Impact of Detergent-Based Decellularization Methods on Porcine Tissues for Heart Valve Engineering.
abstract: To date an optimal decellularization protocol of heart valve leaflets (HVL) and pericardia (PER) with an adequate preservation of the extracellular matrix (ECM) is still lacking. This study compares a 4 day Triton X-100-based protocol with faster SDC-based protocols for the decellularization of cardiac tissues. Decellularized and non-treated HVL and PER were processed for histological, biochemical and mechanical analysis to determine the effect of these agents on the structure, ECM components, and biomechanical properties. Tissues treated with SDC-based protocols still showed nuclear material, whereas tissues treated with Triton X-100 1% + ENZ ± TRYP were completely cell free. For both decellularized tissues, an almost complete washout of glycosaminoglycans, a reduction of soluble collagen and an alteration of the surface ultrastructure was observed. Interestingly, only the elastic fibers of pericardial tissue were affected and this tissue had a decreased maximum load. This study showed that both detergents had a similar impact on the ECM. However, Triton X-100 1% +DNase/RNase (ENZ) ± Trypsin (TRYP) is the only protocol that generated completely cell free bioscaffolds. Also, our study clearly demonstrated that the decellularization agents have more impact on pericardial tissues than on heart valve leaflets. Thus, for the purpose of tissue engineering of heart valves, it is advisable to use valvular rather than pericardial matrices.
authors: Department of Basic Medical Sciences (Tissue Engineering Group), Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185 - Building Block B3 - 6th Floor, 9000, Ghent, Belgium. annelies.roosens@ugent.be.
authors: Annelies
authors: A
authors: Roosens
authors: Department of Basic Medical Sciences (Tissue Engineering Group), Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185 - Building Block B3 - 6th Floor, 9000, Ghent, Belgium.
authors: Pamela
authors: P
authors: Somers
authors: Heart Centre, University Hospital Ghent, Ghent, Belgium.
authors: Filip
authors: F
authors: De Somer
authors: Department of Basic Medical Sciences (Tissue Engineering Group), Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185 - Building Block B3 - 6th Floor, 9000, Ghent, Belgium.|Department of Histology, Tissue Engineering Group, University of Granada, Granada, Spain.
authors: Victor
authors: V
authors: Carriel
authors: Heart Centre, University Hospital Ghent, Ghent, Belgium.
authors: Guido
authors: G
authors: Van Nooten
authors: Department of Basic Medical Sciences (Tissue Engineering Group), Faculty of Medicine and Health Sciences, Ghent University, De Pintelaan 185 - Building Block B3 - 6th Floor, 9000, Ghent, Belgium.
authors: Ria
authors: R
authors: Cornelissen
mesh_terms: D000818:Animals; D003902:Detergents / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D006351:Heart Valves; D017830:Octoxynol / Q000737:chemistry*; D010496:Pericardium / Q000737:chemistry*; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003902:Detergents; D017830:Octoxynol
keywords: Acellular scaffold; Extracellular matrix; Heart valve leaflet; Pericardium; Sodium deoxycholate; Triton X-100