title: miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE.
abstract: In this study, we investigate whether miR-128 is capable of regulating the apoptosis and proliferation of human U251 glioma cells by downregulating RhoE. The expression of miR-128 was assessed by quantitative polymerase chain reaction in normal brain tissue and glioma samples. A significant downregulation of the expression of miR-128 was detected in glioma in contrast to normal brain tissue. Following the transfection of pre-miR-128 and anti-miR-128 into U251 cells, the high expression of miR-128 could inhibit proliferation and induce apoptosis in U251 cells, and those effects could be restored by miR-128 knockdown. To analyze the regulation mechanism of miR-128, TargetScan, miRanda and PicTar were used to ascertain whether RhoE was a potential target gene. Next, luciferase activity assay and western blot analysis confirmed that RhoE was a direct and specific target gene of miR-128. The advanced effects of pre-miR-128 on the apoptosis and proliferation of U251 cells were reversed by the upregulation of RhoE expression. In summary, aberrantly expressed miR-128 regulates apoptosis and proliferation in human glioma U251 cells partly by directly targeting RhoE. This finding may offer a new potential therapeutic strategy for the treatment of glioma.
authors: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Chao
authors: C
authors: Shang
authors: Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors: Yang
authors: Y
authors: Hong
authors: Department of Central Laboratory, School of Stomatology, China Medical University, Shenyang, Liaoning 110007, P.R. China.
authors: Yan
authors: Y
authors: Guo
authors: Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.
authors: Yun-Hui
authors: YH
authors: Liu
authors: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Yi-Xue
authors: YX
authors: Xue
keywords: RhoE; U251 cell; glioma; miR-128