title: DNA methyltransferase 1, 3a, and 3b expression in hepatitis C associated human hepatocellular carcinoma and their clinicopathological association.
abstract: Identification of biomarker will obligate a substantial influence on various cancer management and treatment. We hypothesize that genetic/proteomic and epigenetic studies should be uncovering modifications which may be independently or jointly affect the expression of the genes that are involved in the progression of liver cancer (LC). For this purpose, we examined the effect of expressional changes of DNMTs on HCV infected LC of different genotypes. We found that both mRNA and protein expression levels of DNMT1, 3a, and 3b were upregulated in genotype 1b and 3a HCV infected patients as compared to control. However, DNMT3b mRNA levels did not change in genotypes 2a, 3, and 4, but were upregulated at the protein level by genotype 1b, 2a, and 3a. Furthermore, no significant changes were observed for DNMTs investigated in sample expressing the genotypes 5 and 6. Our findings suggest that HCV at least in part by altering DNMTs expression may play a significant role in HCC progression.
authors: The Karachi Institute of Biotechnology and Genetic Engineering, (KIBGE), University of Karachi, Karachi, Pakistan.
authors: Nadir Naveed
authors: NN
authors: Siddiqui
authors: Department of Biochemistry, University of Karachi-Pakistan, Karachi, Pakistan.|Department of Molecular Genetics, Dr. Ziauddin Hospital, North Nazimabad, Karachi, 74700, Pakistan.
authors: Ahtesham
authors: A
authors: Ul Haq
authors: Department of Molecular Genetics, Dr. Ziauddin Hospital, North Nazimabad, Karachi, 74700, Pakistan.
authors: Owais Ali
authors: OA
authors: Siddiqui
authors: Department of Molecular Genetics, Dr. Ziauddin Hospital, North Nazimabad, Karachi, 74700, Pakistan. rizmakhan@hotmail.com.|Department of Biochemistry, Ziauddin University, Clifton, Karachi, 75600, Pakistan. rizmakhan@hotmail.com.
authors: Rizma
authors: R
authors: Khan
mesh_terms: D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000821:virology; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D019175:DNA Methylation; D000090205:DNA Methyltransferase 3A; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D004790:Enzyme Induction; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000150:complications* / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000821:virology; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D014412:Tumor Virus Infections / Q000821:virology*; D015854:Up-Regulation; D000097584:DNA Methyltransferase 3B
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000097584:DNA Methyltransferase 3B; C000717967:DNMT3A protein, human; C000619380:DNMT1 protein, human
keywords: 3a and 3b; DNA methyltransferase 1; Hepatocellular carcinoma; Real time PCR; Two dimensional gel electrophoresis; Western blotting