title: Binding and detoxification of chlorpyrifos by lactic acid bacteria on rice straw silage fermentation.
abstract: This investigation examined the reduction of pesticide residues on straw inoculated with lactic acid bacteria (LAB) during ensiling. Lactobacillus casei WYS3 was isolated from rice straw that contained pesticide residues. Non-sterilized rice straw, which was inoculated with L. casei WYS3, showed increased removal of chlorpyrifos after ensiling, compared with rice straw that was not inoculated with L. casei WYS3 or sterilized rice straw. In pure culture, these strains can bind chlorpyrifos as indicated by high-performance liquid chromatography analysis. Viable L. casei WYS3 was shown to bind 33.3-42% of exogenously added chlorpyrifos. These results are similar to those of acid-treated cells but less than those of heat-treated cells, which were found to bind 32.0% and 77.2% of the added chlorpyrifos respectively. Furthermore, gas chromatography-mass spectrometry analysis determined that L. casei WYS3 detoxified chlorpyrifos via P-O-C cleavage. Real-time polymerized chain reaction analysis determined that organophosphorus hydrolase gene expression tripled after the addition of chlorpyrifos to LAB cultures, compared with the control group (without chlorpyrifos). This paper highlights the potential use of LAB starter cultures for the detoxification and removal of chlorpyrifos residues in the environment.
authors: a Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University , Nanjing , China.
authors: Yan-Su
authors: YS
authors: Wang
authors: a Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University , Nanjing , China.
authors: Tian-Hao
authors: TH
authors: Wu
authors: a Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University , Nanjing , China.
authors: Yao
authors: Y
authors: Yang
authors: a Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University , Nanjing , China.
authors: Cen-Ling
authors: CL
authors: Zhu
authors: b Institute of Livestock Science, Jiangsu Academy of Agricultural Sciences , Nanjing , China.
authors: Cheng-Long
authors: CL
authors: Ding
authors: a Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University , Nanjing , China.
authors: Chuan-Chao
authors: CC
authors: Dai
mesh_terms: D002681:China; D004390:Chlorpyrifos / Q000378:metabolism*; D005285:Fermentation; D019344:Lactic Acid / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D012275:Oryza / Q000737:chemistry* / Q000382:microbiology*; D010573:Pesticide Residues / Q000378:metabolism*; D012820:Silage / Q000382:microbiology*
chemical_list: D010573:Pesticide Residues; D019344:Lactic Acid; D004390:Chlorpyrifos
keywords: Silage; bind; chlorpyrifos; detoxification; lactic acid bacteria