title: Detection of creatinine in exhaled breath of humans with chronic kidney disease by extractive electrospray ionization mass spectrometry.
abstract: Exhaled breath contains chemicals that have a diagnostic value in human pathologies. Here in vivo breath analysis of creatinine has been demonstrated by constructing a novel platform based on extractive electrospray ionization mass spectrometry (EESI-MS) without sample pretreatment. Under optimized experimental conditions, the limit of creatinine detection in breath was 30.57 ng L(-1), and the linear range of detection was from 0.3 μg L(-1) to 100 μg L(-1). The concentration range of creatinine in the exhaled breath of 50 volunteers with chronic kidney disease was from 42 pptv to 924 pptv, and the range of the relative standard deviations was from 9.3% to 19.2%. The method provides high sensitivity, high specificity and high speed for semi-quantitative analysis of creatinine in exhaled human breath.
authors: Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, People's Republic of China.
authors: Qian
authors: Q
authors: Zeng
authors: Penghui
authors: P
authors: Li
authors: Yunfeng
authors: Y
authors: Cai
authors: Wei
authors: W
authors: Zhou
authors: Haidong
authors: H
authors: Wang
authors: Jiao
authors: J
authors: Luo
authors: Jianhua
authors: J
authors: Ding
authors: Huanwen
authors: H
authors: Chen
mesh_terms: D000328:Adult; D000368:Aged; D001944:Breath Tests / Q000379:methods*; D003404:Creatinine / Q000032:analysis*; D045853:Exhalation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D051436:Renal Insufficiency, Chronic / Q000378:metabolism*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D055815:Young Adult
chemical_list: D003404:Creatinine