title: Application of SELDI-TOF in N-glycopeptides profiling of the urine from patients with endometrial, ovarian and cervical cancer.
abstract: OBJECTIVE
  Endometrial (ECa), ovarian (OCa) and cervical (CCa) cancers are among 10 of the most common cancers affecting women worldwide. Cancers are known to cause some proteins to be differentially glycosylated or aberrantly excreted in the urine, which can be used as biomarkers. Since ECa, OCa and CCa are difficult to diagnose at the early stage, the aim of the present study was to identify a panel of new biomarkers for early detection of the cancers using surface-enhanced laser desorption/ionization-time-of-flight (SELDI-TOF) technology. Identification of early biomarkers that are specific and efficient can increase the survival rate of the patients.
  
  
  METHODS
  Digested urinary proteins from patients with ECa, OCa and CCa were incubated on the champedak mannose-binding (CMB) lectin-immobilized PS10 chip. The lectin-captured glycopeptides were detected with SELDI-TOF mass spectrometry and followed by biomarker wizard analysis.
  
  
  RESULTS
  Peaks m/z 1201 and 1449 were detected as potential group discriminators. The peak m/z 1201 could distinguish OCa from CCa and ECa and its sensitivity and specificity were 100%. For m/z 1449, it was able to differentiate ECa from the other two types of cancer.
  
  
  CONCLUSIONS
  The findings of this study suggest urinary glycopeptides m/z 1201 and 1449 may serve as potential biomarkers for the early detection of ECa, OCa and CCa, although this requires further extensive validation on clinically representative populations.
authors: a Department of Cell and Molecular Biology , University Putra Malaysia , Serdang , Selangor , Malaysia .
authors: Alan Kang-Wai
authors: AK
authors: Mu
authors: b Department of Obstetrics and Gynaecology .
authors: Boon-Kiong
authors: BK
authors: Lim
authors: a Department of Cell and Molecular Biology , University Putra Malaysia , Serdang , Selangor , Malaysia .|d University of Malaya Centre for Proteomics Research, University of Malaya , Kuala Lumpur , Malaysia.
authors: Norhaniza
authors: N
authors: Aminudin
authors: c Department of Molecular Medicine , and.|d University of Malaya Centre for Proteomics Research, University of Malaya , Kuala Lumpur , Malaysia.
authors: Onn Haji
authors: OH
authors: Hashim
authors: a Department of Cell and Molecular Biology , University Putra Malaysia , Serdang , Selangor , Malaysia .|d University of Malaya Centre for Proteomics Research, University of Malaya , Kuala Lumpur , Malaysia.
authors: Adawiyah Suriza
authors: AS
authors: Shuib
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D016022:Case-Control Studies; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000652:urine; D005260:Female; D005500:Follow-Up Studies; D006020:Glycopeptides / Q000652:urine*; D006023:Glycoproteins / Q000652:urine*; D006031:Glycosylation; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000652:urine; D011379:Prognosis; D040081:Protein Array Analysis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000652:urine
chemical_list: D014408:Biomarkers, Tumor; D006020:Glycopeptides; D006023:Glycoproteins
keywords: Cervical cancer; N-glycopeptide; SELDI-TOF; endometrial cancer; ovarian cancer