title: Tyrosine kinase-mediated axial motility of basal cells revealed by intravital imaging.
abstract: Epithelial cells are generally considered to be static relative to their neighbours. Basal cells in pseudostratified epithelia display a single long cytoplasmic process that can cross the tight junction barrier to reach the lumen. Using in vivo microscopy to visualize the epididymis, a model system for the study of pseudostratified epithelia, we report here the surprising discovery that these basal cell projections--which we call axiopodia--periodically extend and retract over time. We found that axiopodia extensions and retractions follow an oscillatory pattern. This movement, which we refer to as periodic axial motility (PAM), is controlled by c-Src and MEK1/2-ERK1/2. Therapeutic inhibition of tyrosine kinase activity induces a retraction of these projections. Such unexpected cell motility may reflect a novel mechanism by which specialized epithelial cells sample the luminal environment.
authors: Program in Membrane Biology, Nephrology Division and Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.
authors: Jeremy
authors: J
authors: Roy
authors: Program in Membrane Biology, Nephrology Division and Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.
authors: Bongki
authors: B
authors: Kim
authors: Program in Membrane Biology, Nephrology Division and Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.
authors: Eric
authors: E
authors: Hill
authors: Department of Veterinary and Animal Science, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors: Pablo
authors: P
authors: Visconti
authors: Department of Veterinary and Animal Science, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors: Dario
authors: D
authors: Krapf
authors: Program in Membrane Biology, Nephrology Division and Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.
authors: Claudio
authors: C
authors: Vinegoni
authors: Program in Membrane Biology, Nephrology Division and Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.
authors: Ralph
authors: R
authors: Weissleder
authors: Program in Membrane Biology, Nephrology Division and Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.|Department of Veterinary and Animal Science, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors: Dennis
authors: D
authors: Brown
authors: Program in Membrane Biology, Nephrology Division and Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02114, USA.
authors: Sylvie
authors: S
authors: Breton
mesh_terms: D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D022081:Cell Surface Extensions / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D000069416:Intravital Microscopy; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008854:Microscopy, Electron; D009570:Nitriles / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D019108:Tight Junctions / Q000648:ultrastructure*; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000814:Aniline Compounds; D001549:Benzamides; D009570:Nitriles; D011804:Quinolines; C471992:bosutinib; C506614:mirdametinib; D004159:Diphenylamine; D019061:src-Family Kinases