title: Clinical Significance of Viral Genome Persistence in the Myocardium of Patients with Dilated Cardiomyopathy.
abstract: BACKGROUND
  The impact of myocardial viral persistence on the clinical outcome of patients with dilated cardiomyopathy (DCM) is still open to question.
  
  
  METHODS
  Fifty-two patients with DCM were enrolled and followed for a median of 3.8 years with respect to death or heart transplantation. Studied patients were clinically stable for at least 6 months before hospitalization. They underwent coronary angiography and endomyocardial biopsy. Specimens were examined by histo- and immunohistochemistry, and the viral genomes of parvovirus B19, cytomegalovirus (CMV), Coxsackie B virus (CVB), and hepatitis B and C viruses were studied by real-time polymerase chain reaction.
  
  
  RESULTS
  Forty-two out of 52 patients were available for clinical follow-up. The viral genome was detected in the myocardium of 32 out of 42 patients. Among the viruses studied, CMV and CVB were the most frequently found. Nine out of 42 patients achieved the predefined study end point. No statistically significant correlation was found between the presence of a persistent viral genome and study end point. No statistically significant relationship between viral genomes studied and immunohistology results was detected.
  
  
  CONCLUSIONS
  High prevalence of a viral genome in the myocardium of patients with DCM did not have an influence on their long-term clinical outcome.
authors: Department of Cardiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland.
authors: Ewa
authors: E
authors: Nowalany-Kozielska
authors: Monika
authors: M
authors: Kozieł
authors: Dorota
authors: D
authors: Domal-Kwiatkowska
authors: Celina
authors: C
authors: Wojciechowska
authors: Wojciech
authors: W
authors: Jacheć
authors: Damian
authors: D
authors: Kawecki
authors: Andrzej
authors: A
authors: Tomasik
authors: Brygida
authors: B
authors: Przywara-Chowaniec
authors: Ludmiła
authors: L
authors: Węglarz
authors: Edyta
authors: E
authors: Reichman-Warmusz
authors: Romuald
authors: R
authors: Wojnicz
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D002311:Cardiomyopathy, Dilated / Q000175:diagnosis / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D029822:Enterovirus B, Human / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005500:Follow-Up Studies; D016679:Genome, Viral / Q000235:genetics*; D006321:Heart / Q000821:virology*; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification; D014777:Virus Diseases / Q000821:virology*
chemical_list: D004279:DNA, Viral