title: In Vivo Feasibility of Electrostatic Precipitation as an Adjunct to Pressurized Intraperitoneal Aerosol Chemotherapy (ePIPAC).
abstract: Intraperitoneal chemotherapy is limited by tissue penetration. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) has been shown to improve drug uptake by utilizing the physical properties of gas and pressure. This study investigated the effect of adding electrostatic precipitation to further enhance the pharmacologic properties of this technique.
  
  
  
  A comparative study was performed using an in vivo porcine model. There were 3 cases in each group, PIPAC and electrostatic precipitation pressurized intraperitoneal aerosol chemotherapy (ePIPAC), plus 1 negative control comparing intraperitoneal distribution and tissue uptake of 2 tracer substances (toluidine blue and DT01). Tracer uptake was determined by measuring DT01 in tissue and peritoneal fluid at the end of each procedure.
  
  
  
  Electrostatic precipitation of the aerosol was technically feasible in all ePIPAC animals. The aerosol was cleared completely from the visual field within 15 s in the ePIPAC group versus 30 min in the PIPAC group. The peritoneal surface was homogeneously stained in both groups. After 30 min, 1.5 % remaining DT01 was measured in samples of ePIPAC-treated peritoneal fluid versus 15 % in PIPAC animals (p = 0.01). Tissue concentration was increased after ePIPAC versus PIPAC (p = 0.06).
  
  
  
  ePIPAC is technically feasible and improves tissue uptake of 2 tracer substances compared to PIPAC by up to tenfold. Intraperitoneal distribution was homogeneous in both groups. ePIPAC has the potential to allow more efficient drug uptake, further dose reduction, a significant shortening of the time required for PIPAC application, and improved health and safety measures.
authors: Department of Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, Germany.
authors: Tinatin
authors: T
authors: Kakchekeeva
authors: Department of Surgery, Marien Hospital Herne, Ruhr University Bochum, Herne, Germany.
authors: Cedric
authors: C
authors: Demtröder
authors: DNA Therapeutics, Évry, France.
authors: Nirmitha I
authors: NI
authors: Herath
authors: Alesi Surgical Ltd., Cardiff, Wales, UK.
authors: Dominic
authors: D
authors: Griffiths
authors: Welsh Institute for Minimal Access Therapy (WIMAT), Cardiff, Wales, UK.
authors: Jared
authors: J
authors: Torkington
authors: Institute of Pathology, Medical School Hanover, Hannover, Germany.
authors: Wiebke
authors: W
authors: Solaß
authors: Institut Curie, Orsay, France.
authors: Marie
authors: M
authors: Dutreix
authors: Department of Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, Germany. marc.reymond@yahoo.de.|Department of Surgery, Marien Hospital Herne, Ruhr University Bochum, Herne, Germany. marc.reymond@yahoo.de.
authors: Marc A
authors: MA
authors: Reymond
mesh_terms: D000336:Aerosols / Q000008:administration & dosage*; D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry; D011232:Chemical Precipitation; D002784:Cholesterol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000032:analysis; D004396:Coloring Agents / Q000008:administration & dosage; D004247:DNA / Q000008:administration & dosage / Q000032:analysis; D005240:Feasibility Studies; D005260:Female; D008297:Male; D065629:Peritoneal Absorption; D010537:Peritoneum / Q000737:chemistry; D011312:Pressure; D055672:Static Electricity; D013552:Swine; D014048:Tolonium Chloride / Q000008:administration & dosage
chemical_list: D000336:Aerosols; D004396:Coloring Agents; C000611563:DT01 compound; D014048:Tolonium Chloride; D004247:DNA; D002784:Cholesterol