title: [Study of common cytotoxicity of aminoglycoside and fluoroquinolone antibiotics in cell cultures].
abstract: The aim of the study was to identify the overall cytotoxicity of aminoglycosides netilmicin and tobramycin, and fluoroquinolone ciprofloxacin.
  
  
  METHODS
  We used three types of the cells: constantly transformed cell line CHO-K1, normal human skin fibroblasts and cells of normal human conjunctiva Clone 1-5C-4. Antibiotics activity was detected by their influence on cell viability. Quantitative and qualitative methods of evaluation have used to determine viability of the cells (quantitative assessment: a method of cloning cells and colorimetric method for assessing cell proliferation; qualitative assessment: lifetime visual observation under an inverted microscope for morphological status of cells in culture with photofixing).
  
  
  RESULTS
  The most toxic effect for all types of the cells was shown in tobramycin. The least degree of toxicity for all types of the cells was determined in netilmicin.
  
  
  CONCLUSIONS
  Studied antibiotics exert a cytotoxic effect in vitro and differ in their cytotoxic potential.
authors: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064.
authors: O I
authors: OI
authors: Alexandrova
authors: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064.
authors: Y I
authors: YI
authors: Khorolskaya
authors: MNTK 'Eye Microsurgery' named after Acad. SN Fedorov, Beskudnikovsky Boulevard, 5a, Moscow, Russian Federation 127486.
authors: D Y
authors: DY
authors: Maychuk
authors: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064.
authors: M I
authors: MI
authors: Blinova
authors: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064.
authors: O I
authors: OI
authors: Alexandrova
authors: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064.
authors: Y I
authors: YI
authors: Khorolskaya
authors: MNTK 'Eye Microsurgery' named after Acad. SN Fedorov, Beskudnikovsky Boulevard, 59a, Moscow, Russian Federation 127486.
authors: D Y
authors: DY
authors: Maychuk
authors: Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr., 4, St. Petersburg, Russia, 194064.
authors: M I
authors: MI
authors: Blinova
mesh_terms: D000617:Aminoglycosides / Q000009:adverse effects*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology* / Q000187:drug effects; D060527:Corneal Keratocytes / Q000166:cytology* / Q000187:drug effects; D006224:Cricetinae; D024841:Fluoroquinolones / Q000009:adverse effects*; D006801:Humans
chemical_list: D000617:Aminoglycosides; D024841:Fluoroquinolones