title: Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer.
abstract: Given the role of microRNA in colorectal cancer (CRC) progression, we explored the association between microRNA (miRNA) expression and CRC-related prognosis.
  
  
  
  Three types of tissue samples (primary CRC lesions without liver metastasis, primary lesions with liver metastasis, and liver metastatic tissues) were used for miRNA profiling to identify differentially expressed miRNA. Quantitative real-time PCR was used to examine miRNA expression in CRC cells and in tumor tissues.
  
  
  
  MiR-132 was significantly down-regulated in primary CRC tissues with liver metastasis and liver metastatic lesions compared to primary lesions without liver metastasis. Multivariate analysis for overall survival indicated that low miR-132 expression was an independent prognostic factor for CRC patients (overall survival P = 0.040, disease-free survival P = 0.015). Ectopic expression of miR-132 significantly inhibited cell proliferation and cell invasion. The luciferase reporter assay revealed that anoctamin 1 (ANO1) was a direct target of miR-132. Kaplan-Meier survival curves showed that high ANO1 expression was a significant prognostic factor for overall survival of patients with CRC (P = 0.0344).
  
  
  
  Down-regulation of miR-132 is associated with poor prognosis in CRC. ANO1 could be one of the crucial targets of miR-132 in CRC.
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Yukako
authors: Y
authors: Mokutani
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Mamoru
authors: M
authors: Uemura
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Koji
authors: K
authors: Munakata
authors: DNA-chip Development Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita City, Osaka, Japan.
authors: Daisuke
authors: D
authors: Okuzaki
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Naotsugu
authors: N
authors: Haraguchi
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Hidekazu
authors: H
authors: Takahashi
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Junichi
authors: J
authors: Nishimura
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Taishi
authors: T
authors: Hata
authors: Department of Surgery, Suita Municipal Hospital, Suita City, Osaka, Japan.
authors: Kohei
authors: K
authors: Murata
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Ichiro
authors: I
authors: Takemasa
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Tsunekazu
authors: T
authors: Mizushima
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Yuichiro
authors: Y
authors: Doki
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
authors: Masaki
authors: M
authors: Mori
authors: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan. hyamamoto@gesurg.med.osaka-u.ac.jp.|Department of Molecular Pathology, Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan. hyamamoto@gesurg.med.osaka-u.ac.jp.
authors: Hirofumi
authors: H
authors: Yamamoto
mesh_terms: D020413:3' Untranslated Regions; D000075369:Anoctamin-1 / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018572:Disease-Free Survival; D015536:Down-Regulation; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000556:secondary*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D015996:Survival Rate; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D000075369:Anoctamin-1; C547077:MIRN132 microRNA, human; D035683:MicroRNAs