title: Sugar-carrying Polystyrenes Facilitate Harvesting of APCs from MLRs: Possible Application of Sugar-carrying Polystyrenes to Immunotherapy.
abstract: Antigen-presenting cells (APCs) play a pivotal role in cancer immunotherapy. APCs in conventionally used flasks are harvested by enzymatic digestion or cell scraping for application to cancer immunotherapy. However, these methods may impair functional molecules expressed on the APC surface and reduce their effects in cancer immunotherapy. Recently, we found that APCs could be harvested by shaking at 4°C in flasks coated with poly[N-p-vinylbenzyl-O-2-acetoamide-2-deoxy-β-D-glucopyranosyl-(1→4)-2-acetoamide-2-deoxy-β-D-gluconamide] (PVGlcNAc) or a copolymer consisting of sulfonylurea (SU) linked to poly[N-p-vinyl-benzyl-4-O-β-D-galactopyranosyl-D-gluconamide] [P(VLA-co-SU)]. In the present study, we compared the functions of cytotoxic T-lymphocytes (CTLs) induced by APCs generated in PVGlcNAc- or P(VLA-co-SU)-coated flasks and conventional flasks. APCs from PVGlcNAc- or P(VLA-co-SU)-coated flasks showed higher expression of cluster of differentiation (CD)80/86, CD11c, and major histocompatibility complex class II alloantigen I-A(d), and higher cytotoxicity than APCs from conventional flasks. These results suggest that the use of PVGlcNAc- or P(VLA-co-SU)-coated flasks is optimal for harvesting APCs. The generated APCs also have a higher antigen-presenting ability compared to those generated in conventional flasks. Our results may contribute to the development of effective cancer immunotherapies.
authors: Shukoukai Inc., Tokyo, Japan Department of Cancer Therapy and Research Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors: Akira
authors: A
authors: Imaizumi
authors: Department of Cancer Therapy and Research Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan ohnishi@surg1.med.kyushu-u.ac.jp.
authors: Hideya
authors: H
authors: Onishi
authors: Department of Cancer Therapy and Research Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors: Akio
authors: A
authors: Yamasaki
authors: Department of Cancer Therapy and Research Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors: Makoto
authors: M
authors: Kawamoto
authors: Department of Cancer Therapy and Research Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan Fukuoka General Cancer Clinic, Fukuoka, Japan.
authors: Takashi
authors: T
authors: Morisaki
authors: Celagix, Res, Ltd., Kanagawa, Japan.
authors: Mitsuaki
authors: M
authors: Goto
authors: Celagix, Res, Ltd., Kanagawa, Japan.
authors: Masamichi
authors: M
authors: Iwama
authors: Frontier Research Center, Tokyo, Japan.
authors: Toshihiro
authors: T
authors: Akaike
authors: Shukoukai Inc., Tokyo, Japan.
authors: Kenichiro
authors: K
authors: Hasumi
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D039521:CD11c Antigen / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D004187:Disaccharides / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007785:Lactose / Q000031:analogs & derivatives* / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011137:Polystyrenes / Q000378:metabolism*; D011145:Polyvinyls / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; D039521:CD11c Antigen; C497965:Cd86 protein, mouse; D004187:Disaccharides; D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; D011137:Polystyrenes; D011145:Polyvinyls; C000608950:poly(N-p-vinylbenzyl-O-2-acetoamide-2-deoxyglucopyranosyl-(1-4)-2-acetoamide-2-deoxygluconamide); C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose
keywords: CTL; P(VLA-co-SU); PVGlcNAc; antigen-presenting cells; cancer immunotherapy