title: Realization of a label-free electrochemical immunosensor for detection of low density lipoprotein using NiO thin film.
abstract: A label-free electrochemical immunosensor, based on nickel oxide (NiO) thin film, for the detection of low density lipoprotein (LDL) has been proposed. P-type semiconducting NiO thin film was deposited by RF sputtering technique and its properties were investigated by X-ray diffraction and Fourier transform infrared spectroscopy. The NiO thin film was utilized as an efficient matrix for the covalent immobilization of apolipoprotein B-100 antibody using EDC/NHS chemistry. The immunoelectrode, thus prepared, was studied using differential pulse voltammetry, cyclic voltammetry and electrochemical impedance spectroscopy. The impedimetric response of the immunosensor exhibited a high sensitivity of 12 kΩ μM(-1) over a wide linear range (0.018-0.5 μM) of LDL. The long shelf life (18 weeks) and enhanced performance characteristics of the immunosensor demonstrate the excellent ability of the NiO matrix for quantification of LDL at commercial level.
authors: Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India.
authors: Gurpreet
authors: G
authors: Kaur
authors: Physics Department, Miranda House, University of Delhi, Delhi 110007, India.
authors: Monika
authors: M
authors: Tomar
authors: Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India. Electronic address: drguptavinay@gmail.com.
authors: Vinay
authors: V
authors: Gupta
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D053299:Apolipoprotein B-100 / Q000032:analysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D058266:Dielectric Spectroscopy / Q000295:instrumentation; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008077:Lipoproteins, LDL / Q000032:analysis*; D009532:Nickel / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D055910:Antibodies, Immobilized; D053299:Apolipoprotein B-100; D008077:Lipoproteins, LDL; D009532:Nickel; C028007:nickel monoxide
keywords: Electrochemical biosensor; Low density lipoprotein; Nickel oxide; Sputtering