title: Chelating Properties of Peptides from Red Seaweed Pyropia columbina and Its Effect on Iron Bio-Accessibility.
abstract: The aim of this work was to evaluate copper-chelating, iron-chelating and anticariogenic activity of peptides obtained by enzymatic hydrolysis of P. columbina protein concentrate and to study the effects of chelating peptides on iron bio-accessibility. Two hydrolyzates were obtained from P. columbina protein concentrate (PC) using two hydrolysis systems: alkaline protease (A) and alkaline protease + Flavourzyme (AF). FPLC gel filtration profile of PC shows a peak having molecular weight (MW) higher than 7000 Da (proteins). A and AF hydrolyzates had peptides with medium and low MW (1013 and 270 Da), respectively. Additionally, AF presented free amino acids with MW around 82 Da and higher content of His and Ser. Peptides from AF showed the highest chelating properties measured as copper-chelating activity (the lowest β-carotene oxidation rate: Ro; 0.7 min(-1)), iron-chelating activity (33%), and phosphorous and Ca(2+) release inhibition (87 and 81%, respectively). These properties could indicate antioxidant properties, promotion of iron absorption and anticariogenic activity, respectively. In fact, hydrolyzates promoted iron dialyzability (≈ 16%), values being higher than that found for P. columbina seaweed. Chelating peptides from both hydrolyzates can maintain the iron in a soluble and bio-accessible form after gastrointestinal digestion.
authors: Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, 1° de Mayo 3250, (3000), Santa Fe, República Argentina. rec_704@yahoo.com.ar.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (C1425FQB), Ciudad Autónoma de Buenos Aires, República Argentina. rec_704@yahoo.com.ar.
authors: Raúl E
authors: RE
authors: Cian
authors: Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, 1° de Mayo 3250, (3000), Santa Fe, República Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (C1425FQB), Ciudad Autónoma de Buenos Aires, República Argentina.
authors: Antonela G
authors: AG
authors: Garzón
authors: Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Norte, Km 33.5, Tablaje Catastral 13615, Colonia Chuburná de Hidalgo Inn, 97203, Mérida, YUC, Mexico.
authors: David Betancur
authors: DB
authors: Ancona
authors: Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Norte, Km 33.5, Tablaje Catastral 13615, Colonia Chuburná de Hidalgo Inn, 97203, Mérida, YUC, Mexico.
authors: Luis Chel
authors: LC
authors: Guerrero
authors: Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, 1° de Mayo 3250, (3000), Santa Fe, República Argentina.|Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (C1425FQB), Ciudad Autónoma de Buenos Aires, República Argentina.
authors: Silvina R
authors: SR
authors: Drago
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry* / Q000302:isolation & purification; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D006868:Hydrolysis; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D000461:Rhodophyta / Q000737:chemistry*; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D001426:Bacterial Proteins; D002614:Chelating Agents; D010455:Peptides; D010940:Plant Proteins; D019207:beta Carotene; D010758:Phosphorus; D003300:Copper; D007501:Iron; D010450:Endopeptidases; C412470:flavourzyme; C087822:alkaline protease; D002118:Calcium
keywords: Anticariogenic properties; Chelating peptides; Iron bio-accessibility; Red seaweeds