title: Alveolar recruitment of ficolin-3 in response to acute pulmonary inflammation in humans.
abstract: BACKGROUND
  Ficolins serve as soluble recognition molecules in the lectin pathway of complement. They are known to participate in the systemic host-response to infection but their role in local pulmonary defence is still incompletely understood. The purpose of this study was to clarify whether acute lung and systemic inflammation induce recruitment of lectins in humans.
  
  
  METHODS
  Fifteen healthy volunteers received LPS intravenously (IV) or in a lung subsegment on two different occasions. Volunteers were evaluated by consecutive blood samples and by bronchoalveolar lavage 2, 4, 6, 8, or 24h after LPS (n=3 in all groups), and gene expression patterns and protein levels of mannose-binding lectin (MBL) and ficolins were determined.
  
  
  RESULTS
  Endobronchial LPS was associated with an increase in alveolar ficolin-3 and MBL levels (p<0.04 and p<0.001, respectively). IV LPS elicited a pronounced acute phase response with an increase in CRP (p<0.001) and plasma ficolin-1 protein levels (p<0.001), whereas no changes were observed in ficolin-1 gene expression patterns (p=0.11) or plasma protein levels of MBL, ficolin-2, or ficolin-3.
  
  
  CONCLUSIONS
  LPS induces a tissue-specific recruitment of ficolin-3 and ficolin-1 in the lung and systemic compartment, respectively, suggesting an important role of distinct lectin complement pathway initiators in the local pulmonary and systemic host defence.
authors: Department of Intensive Care, University Hospital Rigshospitalet, Copenhagen Ø, Denmark. Electronic address: drplovsing@gmail.com.
authors: Ronni R
authors: RR
authors: Plovsing
authors: Department of Clinical Physiology, Nuclear Medicine & PET, University Hospital Rigshospitalet, Copenhagen Ø, Denmark; Centre of Inflammation and Metabolism, University Hospital Rigshospitalet, Copenhagen Ø, Denmark.
authors: Ronan M G
authors: RM
authors: Berg
authors: Department of Clinical Immunology, Laboratory of Molecular Medicine, University Hospital Rigshospitalet, Copenhagen Ø, Denmark.
authors: Lea
authors: L
authors: Munthe-Fog
authors: Centre for Clinical Education, University of Copenhagen and the Capital Region of Denmark, Copenhagen, Denmark.
authors: Lars
authors: L
authors: Konge
authors: Department of Lung Transplantation, University Hospital Rigshospitalet, Copenhagen Ø, Denmark.
authors: Martin
authors: M
authors: Iversen
authors: Department of Neuroanaesthesiology, Neurointensive Care Unit, University Hospital Rigshospitalet, Copenhagen Ø, Denmark; Centre of Inflammation and Metabolism, University Hospital Rigshospitalet, Copenhagen Ø, Denmark.
authors: Kirsten
authors: K
authors: Møller
authors: Department of Clinical Immunology, Laboratory of Molecular Medicine, University Hospital Rigshospitalet, Copenhagen Ø, Denmark.
authors: Peter
authors: P
authors: Garred
mesh_terms: D000328:Adult; D015415:Biomarkers; D002097:C-Reactive Protein; D037582:Complement Pathway, Mannose-Binding Lectin / Q000276:immunology; D006801:Humans; D037102:Lectins / Q000276:immunology* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D037601:Mannose-Binding Lectin / Q000378:metabolism; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D011014:Pneumonia / Q000276:immunology* / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000276:immunology* / Q000378:metabolism*; D055815:Young Adult; D000097604:Ficolins
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D037102:Lectins; D008070:Lipopolysaccharides; D037601:Mannose-Binding Lectin; D000097604:Ficolins
keywords: Complement; Ficolin-1; Ficolin-3; Inflammation; Lectin pathway; Lipopolysaccharide; Mbl