title: RGD and polyhistidine tumor homing peptides potentiates the action of human Maspin as an antineoplastic candidate.
abstract: Maspin, a non-inhibitory member of serine protease family, acts as an effective tumor suppressor by inhibiting cell inhesion and mobility. We found that exogenous wild-type rMaspin had a low effect on tumor growth in vivo. However, when the peptide Arg-Gly-Asp-hexahistidine (RGD-6His) was introduced into rMaspin, the modified rMaspin showed significant inhibitory activity in angiogenic assays and tumor-bearing animal models. Overall, our data suggested that both the RGD and hexahistidine fragments contributed to improve the fusion protein activity and polyhistidine peptide could be considered as flexible linker to separate RGD and Maspin moieties to avoid function interference. Besides, it is an efficient tag to achieve purified recombinant proteins. Furthermore, rMaspin fusing with RGD and hexahistidine could be a viable anticancer candidate.
authors: Nantong University, School of Pharmacy, Qixiu Road 19, Nantong, 226001, Jiangsu, People's Republic of China. yinrunting@126.com.
authors: Runting
authors: R
authors: Yin
authors: Ningxia Medical University, School of Laboratory Medicine, Yinchuan, 750004, Ningxia, People's Republic of China.
authors: Le
authors: L
authors: Guo
authors: Nantong University, School of Pharmacy, Qixiu Road 19, Nantong, 226001, Jiangsu, People's Republic of China.
authors: Jie
authors: J
authors: Zhang
authors: Nantong University, School of Pharmacy, Qixiu Road 19, Nantong, 226001, Jiangsu, People's Republic of China.
authors: Guangzhao
authors: G
authors: Liu
authors: Nantong University, School of Pharmacy, Qixiu Road 19, Nantong, 226001, Jiangsu, People's Republic of China.
authors: Wenjuan
authors: W
authors: Yao
authors: Nantong University, School of Pharmacy, Qixiu Road 19, Nantong, 226001, Jiangsu, People's Republic of China.
authors: Hongyan
authors: H
authors: Zhu
authors: Nantong University, School of Pharmacy, Qixiu Road 19, Nantong, 226001, Jiangsu, People's Republic of China.
authors: Xiaole
authors: X
authors: Xu
authors: Nantong University, School of Pharmacy, Qixiu Road 19, Nantong, 226001, Jiangsu, People's Republic of China. zhang_wei605@163.com.
authors: Wei
authors: W
authors: Zhang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006639:Histidine / Q000737:chemistry / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D015843:Serpins / Q000737:chemistry / Q000494:pharmacology*; D023041:Xenograft Model Antitumor Assays
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D011994:Recombinant Proteins; C085344:SERPIN-B5; D015843:Serpins; C033223:polyhistidine; D006639:Histidine; C047981:arginyl-glycyl-aspartic acid
keywords: Antiangiogenesis; Anticancer; Hexahistidine; RMaspin