title: Characterization of the nuclear import mechanisms of HSV-1 UL31.
abstract: As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the present study, by utilizing live cells fluorescent microscopy and co-immunoprecipitation assays, the nuclear import of UL31 was characterized to be dependent on Ran-, importin α1- and transportin-1-mediated pathway. Therefore, these results will promote the understanding of UL31-mediated biological functions in HSV-1 infection cycle.
authors: Mingsheng
authors: M
authors: Cai
authors: Jiang
authors: J
authors: Si
authors: Xiaowei
authors: X
authors: Li
authors: Zhancheng
authors: Z
authors: Zeng
authors: Meili
authors: M
authors: Li
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D057809:HEK293 Cells; D018259:Herpesvirus 1, Human / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D028901:alpha Karyopherins / Q000378:metabolism; D028961:beta Karyopherins / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; C108121:TNPO1 protein, human; C108721:UL31 protein, Human herpesvirus 1; D014764:Viral Proteins; D028901:alpha Karyopherins; D028961:beta Karyopherins; C087978:karyopherin alpha 2