title: Inhibition of the FACT Complex Reduces Transcription from the Human Cytomegalovirus Major Immediate Early Promoter in Models of Lytic and Latent Replication.
abstract: The successful colonization of the majority of the population by human cytomegalovirus is a direct result of the virus's ability to establish and, more specifically, reactivate from latency. The underlying cellular factors involved in viral reactivation remain unknown. Here, we show that the host complexfacilitateschromatintranscription (FACT) binds to the major immediate early promoter (MIEP) and that inhibition of this complex reduces MIEP transactivation, thus inhibiting viral reactivation.
authors: Department of Microbiology and Immunology, University at Buffalo-SUNY, Buffalo, New York, USA.
authors: Christine M
authors: CM
authors: O'Connor
authors: FORGE Life Science, LLC, Baruch S. Blumberg Research Institute, Doylestown, Pennsylvania, USA.
authors: Masatoshi
authors: M
authors: Nukui
authors: Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York, USA.
authors: Katerina V
authors: KV
authors: Gurova
authors: FORGE Life Science, LLC, Baruch S. Blumberg Research Institute, Doylestown, Pennsylvania, USA eain.murphy@bblumberg.org.
authors: Eain A
authors: EA
authors: Murphy
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early; D006801:Humans; D008954:Models, Biological; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017735:Virus Latency; D057074:Virus Release; D014779:Virus Replication
chemical_list: D014764:Viral Proteins