title: Polyacrylamide Gel-Entrapped Maltase: An Excellent Design of Using Maltase in Continuous Industrial Processes.
abstract: Bacterial maltase catalyzes the hydrolysis of maltose and is known as one of the most significant hydrolases. It has several applications in different industrial processes but widely used in food fermentation technology and alcohol production. In the current study, entrapment technique was comprehensively examined using polyacrylamide gel as a matrix support to improve the stability and catalytic efficiency of maltase for continuous use. Maximum entrapment yield of maltase was achieved at 10 % polyacrylamide concentration with 3.0-mm bead size. Optimized conditions indicated an increase in the reaction temperature from 45 to 55 °C after maltase entrapment while no change was observed in the reaction time and pH. An increase in the K m value of entrapped maltase was attained whereas V max value decreased from 8411.0 to 6813.0 U ml(-1) min(-1) with reference to its free counterpart. Entrapped maltase showed remarkable thermal stability and retained 16 % activity at 70 °C even after 120.0 min. Entrapped maltase also exhibited excellent recycling efficiency up to eight consecutive reaction cycles. With respect to economic feasibility, entrapped maltase indicates its high potential to be used in various biotechnological applications.
authors: Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), KPK, Pakistan. asif_biotech33@yahoo.com.|The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan. asif_biotech33@yahoo.com.
authors: Muhammad Asif
authors: MA
authors: Nawaz
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
authors: Afsheen
authors: A
authors: Aman
authors: Department of Chemistry, University of Turbat, Kech, Baluchistan, Pakistan.
authors: Haneef Ur
authors: HU
authors: Rehman
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
authors: Zainab
authors: Z
authors: Bibi
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
authors: Asma
authors: A
authors: Ansari
authors: Department of Animal Science, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), KPK, Pakistan.
authors: Ziaul
authors: Z
authors: Islam
authors: Jamil-ur-Rahman Center for Genome Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
authors: Ishtiaq Ahmad
authors: IA
authors: Khan
authors: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
authors: Shah Ali Ul
authors: SA
authors: Qader
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D008320:Maltose / Q000096:biosynthesis* / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; C016680:polyacrylamide gels; D008320:Maltose; D000520:alpha-Glucosidases
keywords: Entrapment; Maltase; Polyacrylamide; Recycling efficiency; Thermal stability