title: A Continuous Procedure Based on Column Chromatography to Purify Anthocyanins from Schisandra chinensis by a Macroporous Resin plus Gel Filtration Chromatography.
abstract: In our previous study, as natural food colorants and antioxidants, the color and content stabilities of Schisandra chinensis (S. chinensis) anthocyanins were investigated. In this work, the purification process parameters of S. chinensis anthocyanins using a macroporous resin and gel filtration chromatography were evaluated. The optimized parameters of static adsorption and desorption were as follows. The selected resin is HPD-300 (nonpolar copolymer styrene type resin), and the anthocyanins adsorption saturation capacity of HPD-300 resin was 0.475 mg/g dry resin. Adsorption time was 4 h, and 0.517 mg/mL of S. chinensis anthocyanins was adsorbed on the resin column with a flow rate of 39 mL/h (3 BV/h). After adsorption, the anthocyanins were completely desorpted with 2.5 BV of 90% (v/v) ethanol solution, and the desorption flow rate was 13 mL/h (1 BV/h). After purification by dynamic adsorption and desorption, the anthocyanins content in the effluent increased from 47.6 mg/g to 128.4 mg/g, the purity of anthocyanins increased six-fold from 5.08% to 30.43%, and the anthocyanins recovery was 96.5%. The major constituent of S. chinensis anthocyanins was isolated with Bio-Gel P2 gel filtration chromatography, and it was detected by liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS) as cyanidin-3-O-xylosylrutinoside. Moreover, the antioxidant activities of S. chinensis anthocyanins were investigated. After purification using the HPD-300 resin, the antioxidant activities of anthocyanins were increased 1.2-fold (FRAP) and 1.7-fold (ABTS).
authors: College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China. yuedaran@163.com.
authors: Daran
authors: D
authors: Yue
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. ylmanefu@163.com.
authors: Lei
authors: L
authors: Yang
authors: College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China. liushouxin@126.com.
authors: Shouxin
authors: S
authors: Liu
authors: College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China. nefulijian@163.com.
authors: Jian
authors: J
authors: Li
authors: College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China. liwei19820927@126.com.|Key Laboratory of Wood Science and Technology of Zhejiang Province, Zhejiang Agriculture and Forestry University, Hangzhou, Lin'an 311300, China. liwei19820927@126.com.
authors: Wei
authors: W
authors: Li
authors: College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China. mchmchmchmch@163.com.
authors: Chunhui
authors: C
authors: Ma
mesh_terms: D000327:Adsorption; D000872:Anthocyanins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002850:Chromatography, Gel / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D012116:Resins, Plant / Q000032:analysis; D012117:Resins, Synthetic / Q000032:analysis*; D032141:Schisandra / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010936:Plant Extracts; D012116:Resins, Plant; D012117:Resins, Synthetic
keywords: LC-ESI-MS; Schisandra chinensis; anthocyanins; antioxidant activities; gel filtration chromatography; macroporous resin