title: Highly Selective Detection of 5-Methylcytosine in Genomic DNA Based on Asymmetric PCR and Specific DNA Damaging Reagents.
abstract: DNA methylation is a significant epigenetic modification of the genome that is involved in regulating many cellular processes. An increasing number of human diseases have been discovered to be associated with aberrant DNA methylation, and aberrant DNA methylation has been deemed to be a potential biomarker for diseases such as cancers. A safe, nontoxic, and sensitive method for accurate detection of 5-methylcytosine in genomic DNA is extremely useful for early diagnosis and therapy of cancers. In this paper, we established a novel system to detect 5-methylcytosine, which is based on bisulfite treatment, asymmetric PCR, and specific DNA damaging reagents. Our method could be used for identifying the loci of 5mC in genomic DNA and detecting the DNA methylation levels in tissues as well.
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Yafen
authors: Y
authors: Wang
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Chaoxing
authors: C
authors: Liu
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Tianlu
authors: T
authors: Wang
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Tingting
authors: T
authors: Hong
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Haomiao
authors: H
authors: Su
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Shuyi
authors: S
authors: Yu
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Hongwei
authors: H
authors: Song
authors: Zhongnan Hospital, Wuhan University , Wuhan, Hubei 430071, P. R. China.
authors: Songmei
authors: S
authors: Liu
authors: Zhongnan Hospital, Wuhan University , Wuhan, Hubei 430071, P. R. China.
authors: Xin
authors: X
authors: Zhou
authors: School of Chemistry and Environmental Engineering, Wuhan Institute of Technology , Wuhan, Hubei 430073, P. R. China.
authors: Wuxiang
authors: W
authors: Mao
authors: College of Chemistry and Molecular Sciences, Institute of Advanced Studies, Wuhan University , Wuhan, Hubei 430072, P. R. China.
authors: Xiang
authors: X
authors: Zhou
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis*; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D019175:DNA Methylation; D007202:Indicators and Reagents / Q000737:chemistry; D057230:Limit of Detection; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D007202:Indicators and Reagents; D044503:5-Methylcytosine; D004247:DNA