title: Automodification of PARP and fatty acid-based membrane lipidome as a promising integrated biomarker panel in molecular medicine.
abstract: OBJECTIVE
  Establishing by statistical analyses whether the analyses of auto-modified poly(ADP-ribose)polymerase and erythrocyte membrane fatty acid composition (Fat Profile(®)), separately or in tandem, help monitoring the physio-pathology of the cell, and correlate with diseases, if present.
  
  
  METHODS
  Ninety five subjects were interviewed and analyzed blindly. Blood lymphocytes and erythrocytes were prepared to assay poly(ADP-ribose)polymerase automodification and fatty acid based membrane lipidome, respectively.
  
  
  RESULTS
  Poly(ADP-ribose)polymerase automodification levels confirmed their correlation with DNA damage extent, and allowed monitoring disease activity, upon surgical/therapeutic treatment. Membrane lipidome profiles showed lipid unbalance mainly linked to inflammatory states. Statistically both tests were separately significant, and correlated each other within some pathologies.
  
  
  CONCLUSIONS
  In the laboratory routine, both tests, separately or in tandem, might be a preliminary and helpful step to investigate the occurrence of a given disease. Their combination represents a promising integrated panel for sensible, noninvasive and routine health monitoring.
authors: Department of Biology, University of Naples "Federico II", 80126 Naples, Italy.
authors: Anna Rita
authors: AR
authors: Bianchi
authors: National Research Council (CNR), Institute of Organic Synthesis & Photoreactivity (ISOF), 40129 Bologna, Italy.
authors: Carla
authors: C
authors: Ferreri
authors: Department of Clinical Medicine & Surgery, University of Naples "Federico II", 80135 Naples, Italy.
authors: Simona
authors: S
authors: Ruggiero
authors: Lipinutragen srl, Lipidomic Laboratory, 40129 Bologna, Italy.
authors: Simone
authors: S
authors: Deplano
authors: Lipinutragen srl, Lipidomic Laboratory, 40129 Bologna, Italy.
authors: Valentina
authors: V
authors: Sunda
authors: Department of Clinical Medicine & Surgery, University of Naples "Federico II", 80135 Naples, Italy.
authors: Giuseppe
authors: G
authors: Galloro
authors: Department of Clinical Medicine & Surgery, University of Naples "Federico II", 80135 Naples, Italy.
authors: Cesare
authors: C
authors: Formisano
authors: Department of Biology, University of Naples "Federico II", 80126 Naples, Italy.|National Institute of Biostructures & Biosystems, 00136 Rome, Italy.
authors: Maria Rosaria Faraone
authors: MR
authors: Mennella
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055162:Biocatalysis; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D002462:Cell Membrane / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D050356:Lipid Metabolism; D008214:Lymphocytes / Q000378:metabolism; D055442:Metabolome; D008875:Middle Aged; D062346:Molecular Medicine; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D012372:ROC Curve; D055815:Young Adult
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D011064:Poly Adenosine Diphosphate Ribose; D011065:Poly(ADP-ribose) Polymerases
keywords: DNA damage; epigenetics; lipidomics; membrane phospholipids; poly(ADPribosyl)ation