title: Cooperation of Aspergillus nidulans enzymes increases plant polysaccharide saccharification.
abstract: Efficient polysaccharide degradation depends on interaction between enzymes acting on the main chain and the side chains. Previous studies demonstrated cooperation between several enzymes, but not all enzyme combinations have been explored. A better understanding of enzyme cooperation would enable the design of better enzyme mixtures, optimally profiting from synergistic effects. In this study, we analyzed the cooperation of several enzymes involved in the degradation of xylan, glucan, xyloglucan and crude plant biomass from Aspergillus nidulans by single and combined incubations with their polymeric substrate. Positive effects were observed between most enzymes, although not always to the same extent. Moreover, the tailor made cocktails formulated in this study resulted in efficient release of glucose from plant biomass. This study also serves as an example for the complex cooperation that occurs between enzymes in plant biomass saccharification and how expression in easily-accessible hosts, such as Pichia pastoris, can help in revealing these effects.
authors: State University of West of Paraná (UNIOESTE), Cascavel, Brazil.
authors: Robson
authors: R
authors: Tramontina
authors: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors: Diogo
authors: D
authors: Robl
authors: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors: Gabriela Piccolo
authors: GP
authors: Maitan-Alfenas
authors: Fungal Physiology, CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands. r.devries@cbs.knaw.nl.|Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands. r.devries@cbs.knaw.nl.
authors: Ronald P
authors: RP
authors: de Vries
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D018533:Biomass; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry; D006868:Hydrolysis; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D031786:Saccharum / Q000737:chemistry; D014990:Xylans / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan
keywords: Aspergillus nidulans; Enzymatic cocktails; Polysaccharide hydrolysis; Recombinant enzymes; Saccharification