title: The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongates α1-2-fucosyltransferase.
abstract: A novel α1-2-fucosyltransferase from Thermosynechococcus elongatus BP-1 (Te2FT) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized. It was used in an efficient one-pot multienzyme (OPME) fucosylation system for the high-yield synthesis of human blood group H antigens containing β1-3-linked galactosides and an important human milk oligosaccharide (HMOS) lacto-N-fucopentaose I (LNFP I) on preparative and gram scales. LNFP I was shown to be selectively consumed by Bifidobacterium longum subsp. infantis but not Bifidobacterium animalis subsp. lactis and is a potential prebiotic.
authors: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors: Chao
authors: C
authors: Zhao
authors: Yijing
authors: Y
authors: Wu
authors: Hai
authors: H
authors: Yu
authors: Ishita M
authors: IM
authors: Shah
authors: Yanhong
authors: Y
authors: Li
authors: Jie
authors: J
authors: Zeng
authors: Bin
authors: B
authors: Liu
authors: David A
authors: DA
authors: Mills
authors: Xi
authors: X
authors: Chen
mesh_terms: D001789:Blood Group Antigens / Q000096:biosynthesis* / Q000737:chemistry; D002236:Carbohydrate Conformation; D000458:Cyanobacteria / Q000201:enzymology*; D004789:Enzyme Activation; D005647:Fucosyltransferases / Q000378:metabolism*; D006801:Humans; D008895:Milk, Human / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D001789:Blood Group Antigens; D005647:Fucosyltransferases; D009844:Oligosaccharides; D000097631:Galactoside 2-alpha-L-fucosyltransferase