title: A fluorescent graphitic carbon nitride nanosheet biosensor for highly sensitive, label-free detection of alkaline phosphatase.
abstract: Graphitic C3N4 (g-C3N4) nanosheets provide an attractive option for bioprobes and bioimaging applications. Utilizing highly fluorescent and water-dispersible ultrathin g-C3N4 nanosheets, a highly sensitive, selective and label-free biosensor has been developed for ALP detection for the first time. The developed approach utilizes a natural substrate of ALP in biological systems and thus affords very high catalytic efficiency. This novel biosensor is demonstrated to enable quantitative analysis of ALP in a wide range from 0.1 to 1000 U L(-1) with a low detection limit of 0.08 U L(-1), which is among the most sensitive assays for ALP. It is expected that the developed method may provide a low-cost, convenient, rapid and highly sensitive platform for ALP-based clinical diagnostics and biomedical applications.
authors: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. haotang@hnu.edu.cn jianhuijiang@hnu.edu.cn.
authors: Mei-Hao
authors: MH
authors: Xiang
authors: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. haotang@hnu.edu.cn jianhuijiang@hnu.edu.cn.
authors: Jin-Wen
authors: JW
authors: Liu
authors: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. haotang@hnu.edu.cn jianhuijiang@hnu.edu.cn.
authors: Na
authors: N
authors: Li
authors: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. haotang@hnu.edu.cn jianhuijiang@hnu.edu.cn.
authors: Hao
authors: H
authors: Tang
authors: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. haotang@hnu.edu.cn jianhuijiang@hnu.edu.cn.
authors: Ru-Qin
authors: RQ
authors: Yu
authors: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. haotang@hnu.edu.cn jianhuijiang@hnu.edu.cn.
authors: Jian-Hui
authors: JH
authors: Jiang
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D015374:Biosensing Techniques; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009570:Nitriles / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D009570:Nitriles; C011206:cyanogen; D006108:Graphite; D000469:Alkaline Phosphatase