title: Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests.
abstract: Human mesenchymal stem cells have previously been isolated and characterized from the gingiva, and gingiva-derived stem cells have been applied for tissue engineering purposes. The present study was performed to generate size-controllable stem cell spheroids using concave microwells. Gingiva-derived stem cells were isolated, and the stem cells of 1×105(group A) or 2×105(group B) cells were seeded in polydimethylsiloxane-based, concave micromolds with 600 µm diameters. The morphology of the microspheres was viewed under an inverted microscope, and the changes in the diameter and cell viability were analyzed. The gingiva-derived stem cells formed spheroids in the concave microwells. The diameters of the spheroids were larger in group A compared to group B. No significant changes in shape or diameter were noted with increases in incubation time. Cell viability was higher in group B at each time point when compared with group A. Within the limits of the study, the size-controllable stem cell spheroids could be generated from gingival cells using microwells. The shape of the spheroids and their viability were clearly maintained during the experimental periods.
authors: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
authors: Sung-Il
authors: SI
authors: Lee
authors: Prodizen Inc., Seoul 06100, Republic of Korea.
authors: Seong-Il
authors: SI
authors: Yeo
authors: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
authors: Bo-Bae
authors: BB
authors: Kim
authors: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
authors: Youngkyung
authors: Y
authors: Ko
authors: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
authors: Jun-Beom
authors: JB
authors: Park
keywords: cell culture techniques; cellular spheroids; dimethylpolysiloxanes; stem cells