title: Studies on prevalence of biofilm associated genes and primary observation on sasX gene in clinical isolates of coagulase negative staphylococci (CoNS).
abstract: Coagulase negative staphylococci (CoNS) are nosocomial pathogens that cause indwelling medical device associated infections due to its biofilm forming potential and multiple antibiotic resistance. The current study focused on species identification, antibiotic resistance profile and molecular basis of biofilm formation and attachment of CoNS isolated from clinical samples. Along with this, molecular screening for mecA and newly identified surface colonization protein encoded by sasX gene was also conducted. S. epidermidis (n = 19, 47%) was identified as the most prevalent CoNS species and very interestingly two biofilm forming, mecA positive S. epidermidis isolates were found to carry all the biofilm associated genes screened in this study, which indicates its potential to form the strong biofilm. Another novel observation of the study is the detection of sasX gene in one biofilm positive S. epidermidis isolate. The study also identified one doxycycline resistant mecA positive, multidrug resistant S. haemolyticus isolate. In conclusion, the study signifies the existence of multiple biofilm related genes, multidrug resistance and the presence of sasX gene among clinical isolates of CoNS.
authors: School of Biosciences, Mahatma Gandhi University, Kottayam, India.
authors: Kandammuriyil Radhakrishnan
authors: KR
authors: Soumya
authors: School of Biosciences, Mahatma Gandhi University, Kottayam, India.
authors: Shiji
authors: S
authors: Mathew
authors: MOSC Medical College, Kolencherry, India.
authors: Sheela
authors: S
authors: Sugathan
authors: School of Biosciences, Mahatma Gandhi University, Kottayam, India.
authors: Jyothis
authors: J
authors: Mathew
authors: School of Biosciences, Mahatma Gandhi University, Kottayam, India.
authors: Edayileveettil Krishnankutty
authors: EK
authors: Radhakrishnan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D055499:Catheter-Related Infections / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D003030:Coagulase / Q000172:deficiency / Q000235:genetics; D004318:Doxycycline / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D011446:Prospective Studies; D012867:Skin / Q000382:microbiology; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D041162:Staphylococcus haemolyticus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003030:Coagulase; C487495:MecA protein, Staphylococcus epidermidis; D004318:Doxycycline
keywords: Catheter related sepsis; Coagulase negative staphylococci; methicillin resistance; nosocomial infection; sasX