title: Immunological evaluation of an rsmD-like rRNA methyltransferase from Wolbachia endosymbiont of Brugia malayi.
abstract: Wolbachia is a wonderful anti-filarial target with many of its enzymes and surface proteins (WSPs) representing potential drug targets and vaccine candidates. Here we report on the immunologic response of a drug target, rsmD-like rRNA methyltransferase from Wolbachia endosymbiont of Brugia malayi. The recombinant protein generated both humoral and cell-mediated response in BALB/c mice but compromised its immunity. The humoral response was transient and endured barely for six months in mice with or without B. Malayi challenge. In splenocytes of mice, the key humoral immunity mediating cytokine IL4 was lowered (IL4↓) while IFNγ, the major cytokine mediating cellular immunity was decreased along with upregulation of IL10 cytokine (IFNγ↓, IL10↑). The finding here indicates that the enzyme has low immunogenicity and triggers lowering of cytokine level in BALB/c mice. Interestingly the overall immune profile can be summed up with equivalent response generated by WSP or whole Wolbachia.
authors: Division of Parasitology, CSIR - Central Drug Research Institute, Lucknow, India.
authors: Ajay Kumar
authors: AK
authors: Rana
authors: Division of Parasitology, CSIR - Central Drug Research Institute, Lucknow, India.
authors: Susheela
authors: S
authors: Kushwaha
authors: Division of Parasitology, CSIR - Central Drug Research Institute, Lucknow, India.
authors: Prashant Kumar
authors: PK
authors: Singh
authors: Division of Parasitology, CSIR - Central Drug Research Institute, Lucknow, India. Electronic address: shailja_bhattacharya@cdri.res.in.
authors: Shailja
authors: S
authors: Misra-Bhattacharya
mesh_terms: D000818:Animals; D017178:Brugia malayi / Q000502:physiology; D016207:Cytokines / Q000235:genetics; D005368:Filariasis / Q000517:prevention & control; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D013559:Symbiosis; D020577:Wolbachia / Q000201:enzymology* / Q000276:immunology*
chemical_list: D016207:Cytokines; D011994:Recombinant Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; D008780:Methyltransferases
keywords: BALB/c; Brugia malayi; Immune response; Wolbachia; rsmD-like rRNA-methyltransferase