title: A mesoporous biomaterial for biomimetic crystallization in dentinal tubules without impairing the bonding of a self-etch resin to dentin.
abstract: OBJECTIVE
  CaCO3@mesoporous silica reacted with phosphoric acid (denoted as CCMS-HP) enables the growth of calcium phosphate crystals in dentinal tubules. This study tested whether CCMS-HP could be used to form a biomimetic barrier on the exposed dentin for prevention of dentin sensitivity without impairing the bonding of Single Bond Universal (SBU) self-etch adhesive to the dentin.
  
  
  METHODS
  Twenty-four dentin disks were prepared and divided into three groups: (1) SBU group (n = 8), in which SBU self-etch adhesive was bonded to the dentin disk directly; (2) CCMS-HP group (n = 8), in which CCMS-HP was applied onto the dentin surface; and (3) CCMS-HP/SBU group (n = 8), in which the dentin surface was first treated with CCMS-HP and then boned by SBU. The permeation depth of crystals into the dentinal tubules was examined and measured with a scanning electron microscope. The shear bonding strength of SBU and CCMS-HP/SBU to dentin was also measured.
  
  
  RESULTS
  The mean crystal permeation depth was 35.8 ± 6.9 μm for the CCMS-HP/SBU group and 33.6 ± 12.2 μm for the CCMS-HP group; no significant difference was found between the two groups. Moreover, the mean shear bonding strength was 22.7 ± 6.7 MPa for the CCMS-HP/SBU group and 23.3 ± 7.0 MPa for the SBU group. There was also no significant difference between the two groups.
  
  
  CONCLUSIONS
  CCMS-HP can be used to form a biomimetic barrier for prevention of dentin sensitivity because it neither impedes the bonding of SBU to dentin nor impairs the shear bonding strength between the SBU and dentin.
authors: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: Yu-Chih
authors: YC
authors: Chiang
authors: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: Yin-Lin
authors: YL
authors: Wang
authors: Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
authors: Po-Yen
authors: PY
authors: Lin
authors: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors: Yen-Yi
authors: YY
authors: Chen
authors: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors: Chih-Yu
authors: CY
authors: Chien
authors: Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.
authors: Hong-Ping
authors: HP
authors: Lin
authors: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan. Electronic address: pinlin@ntu.edu.tw.
authors: Chun-Pin
authors: CP
authors: Lin
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D001672:Biocompatible Materials / Q000737:chemistry*; D032701:Biomimetics; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry*; D003188:Composite Resins / Q000737:chemistry*; D003460:Crystallization; D001840:Dental Bonding; D003799:Dental Stress Analysis; D003804:Dentin / Q000648:ultrastructure*; D003807:Dentin Sensitivity / Q000517:prevention & control*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010756:Phosphoric Acids / Q000737:chemistry; D033081:Shear Strength; D013718:Tensile Strength
chemical_list: D001672:Biocompatible Materials; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D010756:Phosphoric Acids; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C030242:phosphoric acid
keywords: biomimetic; crystal growth; dentinal tubule; mesoporous materials; self-etch adhesive