title: Isotope effect analyses provide evidence for an altered transition state for RNA 2'-O-transphosphorylation catalyzed by Zn(2+).
abstract: Solvent D2O and (18)O kinetic isotope effects on RNA 2'-O-transphosphorylation catalyzed by Zn(2+) demonstrate an altered transition state relative to specific base catalysis. A recent model from DFT calculations involving inner sphere coordination to the non-bridging and leaving group oxygens is consistent with the data.
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Shuming
authors: S
authors: Zhang
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Hong
authors: H
authors: Gu
authors: Center for Integrative Proteomics Research, BioMaPS Institute for Quantitative Biology and Department of Chemistry and Chemical Biology, Rutgers University Piscataway, NJ 08854, USA.
authors: Haoyuan
authors: H
authors: Chen
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Emily
authors: E
authors: Strong
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Edward W
authors: EW
authors: Ollie
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Daniel
authors: D
authors: Kellerman
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Danni
authors: D
authors: Liang
authors: Case Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH 44106, USA.
authors: Masaru
authors: M
authors: Miyagi
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Vernon E
authors: VE
authors: Anderson
authors: Department of Chemistry and Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
authors: Joseph A
authors: JA
authors: Piccirilli
authors: Center for Integrative Proteomics Research, BioMaPS Institute for Quantitative Biology and Department of Chemistry and Chemical Biology, Rutgers University Piscataway, NJ 08854, USA.
authors: Darrin M
authors: DM
authors: York
authors: Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. meh2@cwru.edu.
authors: Michael E
authors: ME
authors: Harris
mesh_terms: D002384:Catalysis; D007554:Isotopes; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D007554:Isotopes; D012313:RNA; D015032:Zinc