title: Detection and Analysis of Wnt Pathway Related lncRNAs Expression Profile in Lung Adenocarcinoma.
abstract: Studies have shown that the expression profile of Wnt signaling pathway is very important in lung adenocarcinoma (LAD) and some lncRNAs can regulate the expression of key molecules of Wnt pathway. However, Wnt pathway related lncRNAs are not systematically analyzed and detected in lung adenocarcinoma. We used a high-throughput microarray to compare the lncRNA expression profiles in LAD and corresponding normal tissue (NT) samples. Several candidate Wnt pathway related lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. We found that 232 Wnt pathway related lncRNAs were obviously expressed (≥2-fold change) in lung adenocarcinoma samples and 13 Wnt pathway related lncRNAs were aberrantly expressed in lung adenocarcinoma compared with matched histologically normal lung tissues by qPCR. Among these, RP11-181G12.2 and RP11-89 K21.1 were the most aberrantly expressed lncRNAs. Our study ascertained the expression of Wnt pathway related lncRNAs in lung adenocarcinoma. The results revealed that many Wnt pathway related lncRNAs were differentially expressed in lung adenocarcinoma tissues, suggesting that they may play a key role in tumor development.
authors: Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Jie
authors: J
authors: Chen
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
authors: Lijuan
authors: L
authors: Hu
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
authors: Jian
authors: J
authors: Chen
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
authors: Qinshi
authors: Q
authors: Pan
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
authors: Hongyan
authors: H
authors: Ding
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
authors: Gang
authors: G
authors: Xu
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
authors: Peiwu
authors: P
authors: Zhu
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
authors: Xiusu
authors: X
authors: Wen
authors: Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors: Keta
authors: K
authors: Huang
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang Province, People's Republic of China. wym0577@163.com.
authors: Yumin
authors: Y
authors: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D000368:Aged; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics*; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding
keywords: Expression profile; LncRNAs; Lung adenocarcinoma; Wnt pathway