title: Stem Cell Factor and Interleukin-31 Expression: Association with IgE among Egyptian Patients with Atopic and Nonatopic Bronchial Asthma.
abstract: Bronchial asthma is a chronic inflammatory disorder which remains a significant cause of morbidity. Recently, it has been reported that the stem cell factor (SCF) and interleukin-31 (IL-31) may play a major role in bronchial asthma. The aim of the current study was to study the association of the stem cell factor and interleukin-31 expression with serum immunoglobulin E among Egyptian patients with atopic and nonatopic bronchial asthma. After measuring serum IgE using total enzyme linked immunosorbent assay (ELISA), Ribonucleic acid (RNA) was isolated to determine gene expression of SCF and IL-31 by real-time polymerase chain reaction (PCR). The levels of SCF mRNAs in atopic asthmatic patients' PBMCs were significantly higher than those in controls (p = 0.0001**) and nonatopic asthmatics (p = 0.0001**). There was a high statistical significant difference also with regard to IL-31 between atopic asthmatics and controls (p = 0.0001**) and between them and nonatopic patients (p = 0.014*). There was a strong significant direct correlation between SCF, IL-31 (r = 0.827 and p = 0.0001**) and between both of them and IgE in asthmatics (r = 0.543 and p = 0.0001**) (r = 0.443 and p = 0.0001**), respectively. A direct correlation between SCF, IL-31 and FEV-1/ FVC %, CRP and wheezing existed. These findings suggest that both SCF and IL-31 play an important role in mediating inflammation and enhancing severity of atopic asthma. Augmented inhaled glucocorticoid therapy was associated with significant reductions in SCF and IL-31 mRNA expression as well as improvements in lung function, symptom scores and bronchial hyperresponsiveness to methacholine (PD20) in atopic and nonatopic asthmatics.
authors: a Department of Immunology and Allergy , Medical Research Institute, University of Alexandria , Alexandria , Egypt.
authors: M
authors: M
authors: Moaaz
authors: b Medical Research Institute, University of Alexandria , Alexandria , Egypt.
authors: S
authors: S
authors: Abo El-Nazar
authors: c Alexandria Armed Forces Hospital , Alexandria , Egypt.
authors: M
authors: M
authors: Abd El-Rahman
authors: d Department of Pharmaceutical Science , El-Maamora Hospital , Alexandria , Egypt.
authors: E
authors: E
authors: Soliman
mesh_terms: D000328:Adult; D000368:Aged; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D001249:Asthma / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005938:Glucocorticoids / Q000008:administration & dosage / Q000627:therapeutic use; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007378:Interleukins / Q000097:blood / Q000235:genetics*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D012129:Respiratory Function Tests; D012720:Severity of Illness Index; D019089:Stem Cell Factor / Q000097:blood / Q000235:genetics*
chemical_list: D018927:Anti-Asthmatic Agents; D005938:Glucocorticoids; C502187:IL31 protein, human; D007378:Interleukins; D019089:Stem Cell Factor; D007073:Immunoglobulin E
keywords: Bronchial asthma; IgE; PCR; diagnostic; interleukin- 31; stem cell factor