title: Precocious leaf senescence by functional loss of PROTEIN S-ACYL TRANSFERASE14 involves the NPR1-dependent salicylic acid signaling.
abstract: We report here that Arabidopsis PROTEIN S-ACYL TRANSFERASE14 (PAT14), through its palmitate transferase activity, acts at the vacuolar trafficking route to repress salicylic acid (SA) signaling, thus mediating age-dependent but not carbon starvation-induced leaf senescence. Functional loss of PAT14 resulted in precocious leaf senescence and its transcriptomic analysis revealed that senescence was dependent on salicylic acid. Overexpressing PAT14 suppressed the expression of SA responsive genes. Introducing the SA deficient mutants, npr1-5 and NahG, but not other hormonal mutants, completely suppressed the precocious leaf senescence of PAT14 loss-of-function, further supporting the epistatic relation between PAT14 and the SA pathway. By confocal fluorescence microscopy, we showed that PAT14 is localized at the Golgi, the trans-Golg network/early endosome, and prevacuolar compartments, indicating its roles through vacuolar trafficking. By reporter analysis and real time PCRs, we showed that the expression PAT14, unlike most of the senescence associated genes, is not developmentally regulated, suggesting post-transcriptional regulatory mechanisms on its functionality. We further showed that the maize and wheat homologs of PAT14 fully rescued the precocious leaf senescence of pat14-2, demonstrating that the role of PAT14 in suppressing SA signaling during age-dependent leaf senescence is evolutionarily conserved between dicots and monocots.
authors: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
authors: Xin-Ying
authors: XY
authors: Zhao
authors: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
authors: Jia-Gang
authors: JG
authors: Wang
authors: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
authors: Shi-Jian
authors: SJ
authors: Song
authors: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
authors: Qun
authors: Q
authors: Wang
authors: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
authors: Hui
authors: H
authors: Kang
authors: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
authors: Yan
authors: Y
authors: Zhang
authors: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
authors: Sha
authors: S
authors: Li
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D006056:Golgi Apparatus / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism*; D016415:Sequence Alignment; D015398:Signal Transduction; D014908:Triticum / Q000201:enzymology; D015854:Up-Regulation; D003313:Zea mays / Q000201:enzymology
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D004269:DNA, Bacterial; D007210:Indoleacetic Acids; C105496:NPR1 protein, Arabidopsis; C036483:T-DNA; D002244:Carbon; D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase; D000217:Acyltransferases; C000609136:PAT14 protein, Arabidopsis; D020156:Salicylic Acid