title: A comparison of rumen microbial profiles in dairy cows as retrieved by 454 Roche and Ion Torrent (PGM) sequencing platforms.
abstract: Next generation sequencing (NGS) technology is a widely accepted tool used by microbial ecologists to explore complex microbial communities in different ecosystems. As new NGS platforms continue to become available, it becomes imperative to compare data obtained from different platforms and analyze their effect on microbial community structure. In the present study, we compared sequencing data from both the 454 and Ion Torrent (PGM) platforms on the same DNA samples obtained from the rumen of dairy cows during their transition period. Despite the substantial difference in the number of reads, error rate and length of reads among both platforms, we identified similar community composition between the two data sets. Procrustes analysis revealed similar correlations (M (2) = 0.319; P = 0.001) in the microbial community composition between the two platforms. Both platforms revealed the abundance of the same bacterial phyla which were Bacteroidetes and Firmicutes; however, PGM recovered an additional four phyla. Comparisons made at the genus level by each platforms revealed differences in only a few genera such as Prevotella, Ruminococcus, Succiniclasticum and Treponema (p < 0.05; chi square test). Collectively, we conclude that the output generated from PGM and 454 yielded concurrent results, provided stringent bioinformatics pipelines are employed.
authors: Department of Clinical Studies, University of Pennsylvania , Kennett Square, PA , United States of America.
authors: Nagaraju
authors: N
authors: Indugu
authors: Department of Microbiology and Department of Biostatistics and Epidemiology, University of Pennsylvania , Philadelphia, PA , United States of America.
authors: Kyle
authors: K
authors: Bittinger
authors: Department of Clinical Studies, University of Pennsylvania , Kennett Square, PA , United States of America.
authors: Sanjay
authors: S
authors: Kumar
authors: Department of Clinical Studies, University of Pennsylvania , Kennett Square, PA , United States of America.
authors: Bonnie
authors: B
authors: Vecchiarelli
authors: Department of Clinical Studies, University of Pennsylvania , Kennett Square, PA , United States of America.
authors: Dipti
authors: D
authors: Pitta
keywords: Dairy cows; Next generation sequencing; Rumen microbiome; Transition period