title: Transmission of tuberculosis among people living in the border areas of Poland, the Czech Republic, and Slovakia.
abstract: BACKGROUND
  In 2007, Poland, the Czech Republic, and Slovakia joined the Schengen Agreement, abolishing restrictions on people crossing the borders. Currently, these areas are places of population movements for economic, family, and touristic reasons. This favors the transmission of infectious diseases, including tuberculosis, and requires enhanced control over the spread of the source of infection in the population of patients living in the border areas.
  
  
  OBJECTIVE
  The aim of this study was to investigate the genetic relatedness among Mycobacterium tuberculosis complex strains isolated from patients living in 3 border areas: Poland, the Czech Republic, and Slovakia. PATIENTS AND METHODS The study group consisted of 209 patients with tuberculosis diagnosed and treated between 2007 and 2011 in health care facilities in the Silesia Province in Poland (121 patients [58%]), Žilina in Slovakia (57 [27%]), and the Moravian-Silesian Region in the Czech Republic (31 [15%]). Genotyping of strains was performed using spoligotyping and IS6110-Mtb1-Mtb2 polymerase chain reaction.
  
  
  RESULTS
  Among 209 strains, 23 molecular families (clusters) were identified. Seventeen clusters were identified as national. Six international clusters consisted of 30 strains isolated from patients of various nationalities.
  
  
  CONCLUSIONS
  We identified 6 potential outbreaks of tuberculosis transmission between patients of different nationalities. The circumstances favorable to potential contacts of patients included mainly travelling to the neighboring countries, hospital stays, and addictions. However, there was no evidence of an epidemiological link between these patients, so it may be assumed that if they had come in contact with one another, it was accidental. We observed that the greater incidence of tuberculosis on the Polish territory did not affect the incidence in the Czech Republic or Slovakia over the analysis period.
authors: Monika
authors: M
authors: Kozińska
authors: Jerzy
authors: J
authors: Zientek
authors: Ewa
authors: E
authors: Augustynowicz-Kopeć
authors: Zofia
authors: Z
authors: Zwolska
authors: Jerzy
authors: J
authors: Kozielski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D018153:Czech Republic / Q000453:epidemiology; D004196:Disease Outbreaks; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D011044:Poland / Q000453:epidemiology; D012150:Polymorphism, Restriction Fragment Length; D018154:Slovakia / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000635:transmission*; D055815:Young Adult