title: CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) Cells: Rare Populations in the Human Decidua with Characteristics of MDSC.
abstract: OBJECTIVE
  Human pregnancy needs a remarkable local immune tolerance toward the conceptus. Myeloid-derived suppressor cells (MDSC) are important players promoting cancer initiation and progression by suppressing T-cell functions and thus inducing immune tolerance. Therefore, MDSC were expected within decidua.
  
  
  METHODS
  Subpopulations of CD33(+) immune cells were isolated from human early pregnancy decidua and characterized phenotypically and functionally by microscopy, FACS analysis, RT-PCR, Western blotting and in the coculture with T cells.
  
  
  RESULTS
  Decidua harbors CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) cells which both express arginase, iNOS and IDO and a typical cytokine profile. Both subtypes potently suppress T-cell proliferation and therefore fulfill the criteria of MDSC.
  
  
  CONCLUSIONS
  We characterized a new population of CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) cells in human early pregnancy decidua with properties of classical MDSC and thus potentially being an important player in immune tolerance in pregnancy.
authors: Department of OB/Gyn, University Hospital of Würzburg, Würzburg, Germany.
authors: Catharina
authors: C
authors: Bartmann
authors: Department of OB/Gyn, University Hospital of Würzburg, Würzburg, Germany.
authors: Markus
authors: M
authors: Junker
authors: MVZ Endokrinologikum Hamburg, Hamburg, Germany.
authors: Sabine E
authors: SE
authors: Segerer
authors: Department of OB/Gyn, University Hospital of Würzburg, Würzburg, Germany.
authors: Sebastian F
authors: SF
authors: Häusler
authors: Department of OB/Gyn, University Hospital of Würzburg, Würzburg, Germany.
authors: Mathias
authors: M
authors: Krockenberger
authors: Department of OB/Gyn, University Hospital of Würzburg, Würzburg, Germany.
authors: Ulrike
authors: U
authors: Kämmerer
mesh_terms: D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D060833:Cellular Microenvironment / Q000276:immunology; D018920:Coculture Techniques; D003656:Decidua / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D000072737:Myeloid-Derived Suppressor Cells / Q000502:physiology*; D011247:Pregnancy / Q000276:immunology*; D063268:Sialic Acid Binding Ig-like Lectin 3 / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens; D063268:Sialic Acid Binding Ig-like Lectin 3
keywords: CD33; MDSC; decidua; human pregnancy; immune tolerance