title: Genome-wide survey of Aux/IAA gene family members in potato (Solanum tuberosum): Identification, expression analysis, and evaluation of their roles in tuber development.
abstract: The Auxin/indole-3-acetic acid (Aux/IAA) genes encode short-lived nuclear proteins that are known to be involved in the primary cellular responses to auxin. To date, systematic analysis of the Aux/IAA genes in potato (Solanum tuberosum) has not been conducted. In this study, a total of 26 potato Aux/IAA genes were identified (designated from StIAA1 to StIAA26), and the distribution of four conserved domains shared by the StIAAs were analyzed based on multiple sequence alignment and a motif-based sequence analysis. A phylogenetic analysis of the Aux/IAA gene families of potato and Arabidopsis was also conducted. In order to assess the roles of StIAA genes in tuber development, the results of RNA-seq studies were reformatted to analyze the expression patterns of StIAA genes, and then verified by quantitative real-time PCR. A large number of StIAA genes (12 genes) were highly expressed in stolon organs and in during the tuber initiation and expansion developmental stages, and most of these genes were responsive to indoleacetic acid treatment. Our results suggested that StIAA genes were involved in the process of tuber development and provided insights into functional roles of potato Aux/IAA genes.
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China; Innovation Experimental College, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Junpeng
authors: J
authors: Gao
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Xiaoli
authors: X
authors: Cao
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Shandang
authors: S
authors: Shi
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China; Innovation Experimental College, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Yuling
authors: Y
authors: Ma
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Kai
authors: K
authors: Wang
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Shengjie
authors: S
authors: Liu
authors: School of Life Sciences and Technology, Xidian University, Xi'an, Shaanxi 710071, China.
authors: Dan
authors: D
authors: Chen
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Qin
authors: Q
authors: Chen
authors: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: mahaoli@nwsuaf.edu.cn.
authors: Haoli
authors: H
authors: Ma
mesh_terms: D002874:Chromosome Mapping; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018745:Genome, Plant / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D005810:Multigene Family / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D035281:Plant Tubers / Q000502:physiology*; D020539:Sequence Analysis, Protein; D011198:Solanum tuberosum / Q000502:physiology*; D014018:Tissue Distribution
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators
keywords: Aux/IAA; Auxin; Expression analysis; Potato; Tuber development