title: Simultaneous inhibition of sulfate-reducing bacteria, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl: Applications for microbial enhanced oil recovery.
abstract: Sulfate-reducing bacteria (SRB) are widely existed in oil production system, and its H2S product inhibits rhamnolipid producing bacteria. In-situ production of rhamnolipid is promising for microbial enhanced oil recovery. Inhibition of SRB, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl were investigated. Strain Rhl can simultaneously remove S(2-) (>92%) and produce rhamnolipid (>136mg/l) under S(2-) stress below 33.3mg/l. Rhl reduced the SRB numbers from 10(9) to 10(5)cells/ml, and the production of H2S was delayed and decreased to below 2mg/l. Rhl also produced rhamnolipid and removed S(2-) under laboratory simulated oil reservoir conditions. High-throughput sequencing data demonstrated that addition of strain Rhl significantly changed the original microbial communities of oilfield production water and decreased the species and abundance of SRB. Bioaugmentation of strain Rhl in oilfield is promising for simultaneous control of SRB, removal of S(2-) and enhance oil recovery.
authors: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning Province 110016, China; State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors: Feng
authors: F
authors: Zhao
authors: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning Province 110016, China.
authors: Ji-Dong
authors: JD
authors: Zhou
authors: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors: Fang
authors: F
authors: Ma
authors: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning Province 110016, China.
authors: Rong-Jiu
authors: RJ
authors: Shi
authors: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning Province 110016, China.
authors: Si-Qin
authors: SQ
authors: Han
authors: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors: Jie
authors: J
authors: Zhang
authors: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning Province 110016, China. Electronic address: yzhang207@126.com.
authors: Ying
authors: Y
authors: Zhang
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D044822:Biodiversity; D006017:Glycolipids / Q000096:biosynthesis*; D006862:Hydrogen Sulfide / Q000302:isolation & purification*; D061329:Oil and Gas Fields / Q000382:microbiology*; D010084:Oxidation-Reduction; D044223:Pseudomonas stutzeri / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics; D013431:Sulfates / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D013431:Sulfates; C418382:rhamnolipid; D006862:Hydrogen Sulfide
keywords: Bio-competitive exclusion technology; Engineered strain; High-throughput sequencing; Microbial communities; Simulated reservoir conditions