title: Isatin inhibits the proliferation and invasion of SH-SY5Y neuroblastoma cells.
abstract: Isatin has been shown to initiate apoptotic processes in SH‑SY5Y neuroblastoma cells. The aim of the present study was to investigate whether isatin is also able to alter the proliferation and migratory ability of SH‑SY5Y cells. The results demonstrated that the proportion of SH‑SY5Y cells in G1 phase was significantly increased following treatment with isatin for 48 h with simultaneous downregulation of cyclin D1 expression. In addition, isatin significantly inhibited cell migration and invasion, along with decreases in matrix metalloproteinase (MMP)2 and MMP9 expression. In addition, isatin reduced the levels of phosphorylated signal transducer and activator of transcription 3 (p‑STAT3) in a concentration-dependent manner. These results demonstrated that isatin induces G1‑phase arrest in SH‑SY5Y cells, possibly by decreasing cyclin D1 expression as well as inhibiting their migration and invasiveness, probably by reducing MMP2 and MMP9. These effects may be exerted by isatin via a downregulating the levels of pSTAT3.
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Pingping
authors: P
authors: Xu
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Lin
authors: L
authors: Hou
authors: Department of Pharmacology, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Chuanxia
authors: C
authors: Ju
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Zheng
authors: Z
authors: Zhang
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Wenyan
authors: W
authors: Sun
authors: Department of Pharmacology, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Li
authors: L
authors: Zhang
authors: Department of Laboratory, Women and Children's Hospital of Qingdao, Qingdao, Shandong 266011, P.R. China.
authors: Jinlian
authors: J
authors: Song
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Yuqiang
authors: Y
authors: Lv
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Lu
authors: L
authors: Liu
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Zhixiang
authors: Z
authors: Chen
authors: Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China.
authors: Yanhui
authors: Y
authors: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D007510:Isatin / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D050796:STAT3 Transcription Factor / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D007510:Isatin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9