title: The potential of SNP-based PCR-RFLP capillary electrophoresis analysis to authenticate and detect admixtures of Mediterranean olive oils.
abstract: Authentication and traceability of extra virgin olive oil is a challenging research task due to the complexity of fraudulent practices. In this context, the monovarietal olive oils of Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) require new tests and cutting edge analytical technologies to detect mislabeling and misleading origin. Toward this direction, DNA-based technologies could serve as a complementary to the analytical techniques assay. Single nucleotide polymorphisms are ideal molecular markers since they require short PCR analytical targets which are a prerequisite for forensic applications in olive oil sector. In the present study, a small number of polymorphic SNPs were used with an SNP-based PCR-RFLP capillary electrophoresis platform to discriminate six out of 13 monovarietal olive oils of Mediterranean origin from three different countries, Greece, Tunisia, and Lebanon. Moreover, the high sensitivity of capillary electrophoresis in combination with the DNA extraction protocol lowered the limit of detection to 10% in an admixture of Tsounati in a Koroneiki olive oil matrix.
authors: Department of Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania, Chania, Crete, Greece.
authors: Christos
authors: C
authors: Bazakos
authors: Department of Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania, Chania, Crete, Greece.
authors: Emna
authors: E
authors: Khanfir
authors: Department of Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania, Chania, Crete, Greece.
authors: Mariem
authors: M
authors: Aoun
authors: Department of Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania, Chania, Crete, Greece.
authors: Thodhoraq
authors: T
authors: Spano
authors: Department of Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania, Chania, Crete, Greece.
authors: Zeina El
authors: ZE
authors: Zein
authors: Faculty of Agronomy, Lebanese University, Dekwaneh, Beirut, Lebanon.
authors: Lamis
authors: L
authors: Chalak
authors: Department of Olive and Olive Oil, Lebanese Agricultural Research Institute, Tal Amara, Zahle.
authors: Milad El
authors: ME
authors: Riachy
authors: Department of Laboratory Science and Technology, American University of Science and Technology, Ashrafieh, Lebanon.
authors: Gretta
authors: G
authors: Abou-Sleymane
authors: Olive Tree Institute, Sfax, Tunisia.
authors: Sihem Ben
authors: SB
authors: Ali
authors: Olive Tree Institute, Sfax, Tunisia.
authors: Naziha
authors: N
authors: Grati Kammoun
authors: Department of Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania, Chania, Crete, Greece.
authors: Panagiotis
authors: P
authors: Kalaitzis
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D019083:Mediterranean Region; D000069463:Olive Oil / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D000069463:Olive Oil
keywords: Admixture detection; Capillary electrophoresis; Olive oil; Single Nucleotide Polymorphism