title: Inhibitory activities against rice pathogens of 8-hydroxy-2,4,6-octatriynamide from Agrocybe sp.
abstract: 8-Hydroxy-2,4,6-octatriynamide, a natural polyacetylene with inhibitory activities against rice pathogens, was isolated from the liquid fermentation broth of strain Agrocybe sp. YB2005 during screening for new natural chemical agents to control rice pathogens. 8-hydroxy-2,4,6-octatriynamide was purified by consecutive chromatography over a Cl8 reversed phase silica gel, sephadex LH-20 and silica gel. The chemical structure of 8-hydroxy-2,4,6-octatriynamide was elucidated through spectroscopic analyses, including 1D- and 2D-NMR, ESI mass spectrometry and X-ray single crystal diffraction. Bioassays showed that 8-hydroxy-2,4,6-octatriynamide could significantly inhibit growth of Xanthomonas oryzae with an MIC of 53.1 μM in a 96-well plate and the growth of Rhizoctonia solani at 1.02 mM in a 24-well plate. When rice leaves were inoculated with Magnaporthe grisea and cultured in artificial nutrition liquid containing 0.34 mM 8-hydroxy-2,4,6-octatriynamide, no rice blast was observed. The present study implied that 8-hydroxy-2,4,6-octatriynamide could be a candidate agent against rice pathogens.
authors: Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou City, People's Republic of China. yongbiaozheng@fjnu.edu.cn.|College of Life Sciences, Qishan Campus, Fujian Normal University, Room 104, Building No. 12, Shangjie Town, Minhou County, Fuzhou City, 350117, Fujian Province, People's Republic of China. yongbiaozheng@fjnu.edu.cn.
authors: Yongbiao
authors: Y
authors: Zheng
authors: Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou City, People's Republic of China.|College of Life Sciences, Qishan Campus, Fujian Normal University, Room 104, Building No. 12, Shangjie Town, Minhou County, Fuzhou City, 350117, Fujian Province, People's Republic of China.
authors: Xiaoping
authors: X
authors: Xu
authors: Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou City, People's Republic of China.|College of Life Sciences, Qishan Campus, Fujian Normal University, Room 104, Building No. 12, Shangjie Town, Minhou County, Fuzhou City, 350117, Fujian Province, People's Republic of China.
authors: Yabin
authors: Y
authors: Wu
mesh_terms: D055362:Agrocybe / Q000254:growth & development / Q000378:metabolism*; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry*; D020082:Magnaporthe / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D053279:Polyynes / Q000302:isolation & purification* / Q000494:pharmacology*; D012232:Rhizoctonia / Q000187:drug effects; D013057:Spectrum Analysis; D014974:Xanthomonas / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D053279:Polyynes
keywords: 8-Hydroxy-2,4,6-octatriynamide; Agrocybe sp.; Crystal structure; Rice pathogens