title: MicroRNA-1228(*) inhibit apoptosis in A549 cells exposed to fine particulate matter.
abstract: Studies have reported associations between fine particulate matter (PM2.5) and respiratory disorders; however, the underlying mechanism is not completely clear owing to the complex components of PM2.5. microRNAs (miRNAs) demonstrate tremendous regulation to target genes, which are sensitive to exogenous stimulation, and facilitate the integrative understood of biological responses. Here, significantly modulated miRNA were profiled by miRNA microarray, coupled with bioinformatic analysis; the potential biological function of modulated miRNA were predicted and subsequently validated by cell-based assays. Downregulation of miR-1228-5p (miR-1228(*)) expression in human A549 cells were associated with PM2.5-induced cellular apoptosis through a mitochondria-dependent pathway. Further, overexpression of miR-1228(*) rescued the cellular damages induced by PM2.5. Thus, our results demonstrate that PM2.5-induced A549 apoptosis is initiated by mitochondrial dysfunction and miR-1228(*) could protect A549 cells against apoptosis. The involved pathways and target genes might be used for future mechanistic studies.
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Xiaobo
authors: X
authors: Li
authors: Department of Environmental Health and Endemic Disease Control, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing, 210009, China.
authors: Zhen
authors: Z
authors: Ding
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Chengcheng
authors: C
authors: Zhang
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Xin
authors: X
authors: Zhang
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Qingtao
authors: Q
authors: Meng
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Shenshen
authors: S
authors: Wu
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Shizhi
authors: S
authors: Wang
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Lihong
authors: L
authors: Yin
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China.
authors: Yuepu
authors: Y
authors: Pu
authors: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Dingjiaqiao 87, Nanjing, 210009, China. 101011816@seu.edu.cn.
authors: Rui
authors: R
authors: Chen
mesh_terms: D000072283:A549 Cells; D000393:Air Pollutants / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism; D052638:Particulate Matter / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; C576906:MIRN1228 microRNA, human; D035683:MicroRNAs; D052638:Particulate Matter
keywords: Air pollution; Apoptosis; Environmental toxicology; Fine particulate matter; In vitro toxicology; MicroRNA