title: Data for the crystal structure of APRIL-BAFF-BAFF heterotrimer.
abstract: The TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) modulate B cell function by forming homotrimers and heterotrimers. To determine the structure of a heterotrimer of BAFF and APRIL, these ligands were expressed as a single chain protein in HEK 293 cells, purified by affinity and size exclusion chromatographies, and crystallized. Crystals belonging to the orthorhombic crystal system with a space group of C2221 diffracted to 2.43 Å. Initial structural solution was obtained by the molecular replacement method, and the structure was further refined to an R factor of 0.179 and free R factor of 0.234. The atomic coordinates and structure factors have been deposited into the Protein Data Bank (accession code 4ZCH).
authors: Proteros Biostructures GmbH, D-82152 Planegg, Germany.
authors: Klaus
authors: K
authors: Maskos
authors: Proteros Biostructures GmbH, D-82152 Planegg, Germany.
authors: Alfred
authors: A
authors: Lammens
authors: EMD Serono Research & Development Institute, Billerica, MA 01821, U.S.A.
authors: Seng-Lai
authors: SL
authors: Tan
authors: Merck KGaA, D-64293 Darmstadt, Germany.
authors: Henry
authors: H
authors: Hess
authors: Merck Serono S.A., Aubonne CH-1170, Switzerland.
authors: Wolf
authors: W
authors: Palinsky
authors: Department of Biochemistry, University of Lausanne, CH-1066 Epalinges, Switzerland.
authors: Pascal
authors: P
authors: Schneider
authors: EMD Serono Research & Development Institute, Billerica, MA 01821, U.S.A.
authors: Xuliang
authors: X
authors: Jiang