title: Development of new PCR-based markers specific for chromosome arms of rye (Secale cereale L.).
abstract: PCR-based rye (Secale cereale L.) chromosome-specific markers can contribute to the effective utilization of elite genes of rye in wheat (Triticum aestivum L.) breeding programs. In the present study, 578 new PCR-based rye-specific markers have been developed by using specific length amplified fragment sequencing (SLAF-seq) technology, and 76 markers displayed different polymorphism among rye Kustro, Imperial, and King II. A total of 427 and 387 markers were, respectively, located on individual chromosomes and chromosome arms of Kustro by using a set of wheat-rye monosomic addition lines and 13 monotelosomic addition lines, which were derived from T. aestivum L. 'Mianyang11' × S. cereale L. 'Kustro'. In addition, two sets of wheat-rye disomic addition lines, which were derived from T. aestivum L. var. Chinese Spring × S. cereale L. var. Imperial and T. aestivum L. 'Holdfast' × S. cereale L. var. King II, were used to test the chromosomal specificity of the 427 markers. The chromosomal locations of 281 markers were consistent among the three sets of wheat-rye addition lines. The markers developed in this study can be used to identify a given segment of rye chromosomes in wheat background and accelerate the utilization of elite genes on rye chromosomes in wheat breeding programs.
authors: a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors: Ling
authors: L
authors: Qiu
authors: a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.|b Institute of Ecological Agricultural, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors: Zong-xiang
authors: ZX
authors: Tang
authors: a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors: Meng
authors: M
authors: Li
authors: a Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, People's Republic of China.
authors: Shu-lan
authors: SL
authors: Fu
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D000069600:Plant Breeding; D016133:Polymerase Chain Reaction; D012434:Secale / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: PCR based; blé; chromosome-specific marker; marqueur PCR; marqueur spécifique d’un chromosome; rye; seigle; wheat