title: FGFR3 and Cyclin D3 as urine biomarkers of bladder cancer recurrence.
abstract: OBJECTIVE
  To assess the diagnostic performance of FGFR3 and Cyclin D3 urinary protein levels in detecting bladder cancer recurrence.
  
  
  METHODS
  Urine of 321 patients in follow-up for bladder cancer and 150 non-neoplastic urine controls was included. Cytology, cystoscopy and FGFR3 and Cyclin D3 expression by western blot were performed.
  
  
  RESULTS
  One hundred ten (34.3%) patients had evidence of tumor recurrence. The sensitivity and specificity of cytology/cystoscopy was 80 and 84%, and for FGFR3/Cyclin D3 was of 73 and 90%.
  
  
  CONCLUSIONS
  Combined urinary FGFR3/Cyclin D3 expression shows improved detection rates for bladder cancer recurrence with high specificity and sensitivity, and within the same range of detection shown by cystoscopy, therefore supporting its potential use as noninvasive diagnostic biomarker for bladder cancer recurrence.
authors: Urology Department, Maimonides Institute of Biomedical Research (IMIBIC), Reina Sofia Hospital, University of Cordoba, 14004 Cordoba, Spain.
authors: Ana
authors: A
authors: Blanca
authors: Urology Department, Maimonides Institute of Biomedical Research (IMIBIC), Reina Sofia Hospital, University of Cordoba, 14004 Cordoba, Spain.
authors: Maria J
authors: MJ
authors: Requena
authors: Urology Department, Maimonides Institute of Biomedical Research (IMIBIC), Reina Sofia Hospital, University of Cordoba, 14004 Cordoba, Spain.
authors: Jose
authors: J
authors: Alvarez
authors: Departments of Pathology & Laboratory Medicine, and Urology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors: Liang
authors: L
authors: Cheng
authors: Institute of Pathological Anatomy & Histopathology, Polytechnic University of the Marche Region, Ancona, Italy.
authors: Rodolfo
authors: R
authors: Montironi
authors: Histopathology & Molecular Diagnostics, Careggi University Hospital, 50134, Florence, Italy.
authors: Maria R
authors: MR
authors: Raspollini
authors: Department of Pathology & Surgery, Cordoba University Medical School, 14004 Cordoba, Spain.
authors: Carlos
authors: C
authors: Reymundo
authors: Department of Pathology & Surgery, Cordoba University Medical School, 14004 Cordoba, Spain.
authors: Antonio
authors: A
authors: Lopez-Beltran
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine*; D015153:Blotting, Western; D056743:Cyclin D3 / Q000652:urine*; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000652:urine*; D012372:ROC Curve; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000652:urine*; D001749:Urinary Bladder Neoplasms / Q000652:urine*
chemical_list: D014408:Biomarkers, Tumor; D056743:Cyclin D3; C496365:FGFR3 protein, human; D051498:Receptor, Fibroblast Growth Factor, Type 3
keywords: FGFR3; bladder cancer; cyclin D3; diagnosis; urine; western blot