title: Diagnosis of Periodontal Disease from Saliva Samples Using Fourier Transform Infrared Microscopy Coupled with Partial Least Squares Discriminant Analysis.
abstract: Diagnosis of periodontal disease by Fourier transform infrared (FT-IR) microscopic technique was achieved for saliva samples. Twenty-two saliva samples, collected from 10 patients with periodontal disease and 12 normal volunteers, were pre-processed and analyzed by FT-IR microscopy. We found that the periodontal samples showed a larger raw IR spectrum than the control samples. In addition, the shape of the second derivative spectrum was clearly different between the periodontal and control samples. Furthermore, the amount of saliva content and the mixture ratio were different between the two samples. Partial least squares discriminant analysis was used for the discrimination of periodontal samples based on the second derivative spectrum. The leave-one-out cross-validation discrimination accuracy was 94.3%. Thus, these results show that periodontal disease may be diagnosed by analyzing saliva samples with FT-IR microscopy.
authors: Department of Bioscience and Bioinformatics, Kyushu Institute of Technology.
authors: Satoshi
authors: S
authors: Fujii
authors: Shinobu
authors: S
authors: Sato
authors: Keisuke
authors: K
authors: Fukuda
authors: Toshinori
authors: T
authors: Okinaga
authors: Wataru
authors: W
authors: Ariyoshi
authors: Michihiko
authors: M
authors: Usui
authors: Keisuke
authors: K
authors: Nakashima
authors: Tatsuji
authors: T
authors: Nishihara
authors: Shigeori
authors: S
authors: Takenaka
mesh_terms: D000465:Algorithms; D000704:Analysis of Variance; D016002:Discriminant Analysis; D005583:Fourier Analysis; D064368:Healthy Volunteers; D006801:Humans; D016018:Least-Squares Analysis; D010510:Periodontal Diseases / Q000175:diagnosis*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*