title: Screening Rice Cultivars for Resistance to Bacterial Leaf Blight.
abstract: Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious threats to rice production. In this study, screening of rice for resistance to BLB was carried out at two different times and locations; that is, in a greenhouse during winter and in an open field during summer. The pathogenicity of Xoo race K1 was tested on 32 Korean rice cultivars. Inoculation was conducted at the maximum tillering stage, and the lesion length was measured after 14 days of inoculation. Five cultivars, Hanareum, Namcheon, Samgdeok, Samgang, and Yangjo, were found to be resistant in both the greenhouse and open-field screenings. Expression of the plant defense-related genes JAmyb, OsNPR1, OsPR1a, OsWRKY45, and OsPR10b was observed in resistant and susceptible cultivars by qRT-PCR. Among the five genes tested, only OsPR10b showed coherent expression with the phenotypes. Screening of resistance to Xoo in rice was more accurate when conducted in open fields in the summer cultivation period than in greenhouses in winter. The expression of plant defenserelated genes after bacterial inoculation could give another perspective in elucidating defense mechanisms by using both resistant and susceptible individuals.
authors: Department of Food Security and Agricultural Development, College of Agriculture and Life Science, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors: Agaba Kayihura
authors: AK
authors: Fred
authors: Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors: Gilang
authors: G
authors: Kiswara
authors: Department of Farm Management, College of Agriculture and Life Science, Kyungpook National University, Gyeongbuk 39061, Republic of Korea.
authors: Gihwan
authors: G
authors: Yi
authors: Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors: Kyung-Min
authors: KM
authors: Kim
mesh_terms: D060467:Disease Resistance; D020869:Gene Expression Profiling; D017343:Genes, Plant; D005838:Genotype; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D014974:Xanthomonas / Q000472:pathogenicity*
chemical_list: D010940:Plant Proteins
keywords: Bacterial leaf blight; pathogenicity; resistance; rice; susceptibility