title: Identification and bioactivity evaluation of a novel bradykinin inhibitory peptide from the skin secretion of Chinese large odorous frog, Odorrana livida.
abstract: A novel peptide was isolated from the skin secretion of Chinese large odorous frog, Odorrana livida, and was named as Rana-BI. The cDNA sequencing was obtained by 'shotgun' cloning. The amino acid sequence of the mature peptide was identified as Gly-Leu-Leu-Ser-Gly-Lys-Ser-Val-Lys-Gly-Ser-Ile-OH by automated Edman degradation, and the molecular weight of the peptide was confirmed to be 1144.68 Da by MALDI-TOF and liquid chromatography/MS. Subsequently, the bioactivity of synthetic peptide was evaluated by smooth muscle assay using isolated rat bladder preparation. It was demonstrated that Rana-BI inhibited the contraction of rat bladder induced by bradykinin. Comparing with other peptides by searching from database, the primary structure of Rana-BI showed high similarity with that of an antimicrobial peptide of Rana family (12/12 residues). These data revealed a novel biological function of this peptide.
authors: School of Pharmacy, Nanjing University of Chinese medicine, Nanjing, Jiangsu, China.|School of Pharmacy, Queen's University of Belfast, Belfast, UK.
authors: Kundi
authors: K
authors: Yang
authors: School of Pharmacy, Queen's University of Belfast, Belfast, UK.
authors: Chengbang
authors: C
authors: Ma
authors: School of Pharmacy, Queen's University of Belfast, Belfast, UK.
authors: Mei
authors: M
authors: Zhou
authors: School of Pharmacy, Queen's University of Belfast, Belfast, UK.
authors: Lei
authors: L
authors: Wang
authors: School of Pharmacy, Queen's University of Belfast, Belfast, UK.
authors: Renjie
authors: R
authors: Li
authors: School of Pharmacy, Queen's University of Belfast, Belfast, UK.
authors: Tianbao
authors: T
authors: Chen
authors: School of Pharmacy, Queen's University of Belfast, Belfast, UK.
authors: Chris
authors: C
authors: Shaw
authors: School of Pharmacy, Nanjing University of Chinese medicine, Nanjing, Jiangsu, China.
authors: Wei
authors: W
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D000700:Analgesics / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D001920:Bradykinin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D009812:Odorants; D009842:Oligopeptides / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D011898:Ranidae / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D012867:Skin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D046509:Tissue Culture Techniques; D001743:Urinary Bladder / Q000187:drug effects
chemical_list: D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D018076:DNA, Complementary; D009842:Oligopeptides; D001920:Bradykinin
keywords: Odorrana livida; Rana-BI; bradykinin; sequence; skin secretion