title: Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides.
abstract: Rhodosporidium toruloides AS 2.1389 was tested using different concentrations of acetic acid as a low-cost carbon source for the production of microbial lipids, which are good raw materials for biodiesel production. It grew and had higher lipid contents in media containing 4-20 g/L acetic acid as the sole carbon source, compared with that in glucose-containing media under the same culture conditions. At acetic acid concentrations as high as 20 g/L and the optimal carbon-to-nitrogen ratio (C/N) of 200 in a batch culture, the highest biomass production was 4.35 g/L, with a lipid content of 48.2%. At acetic acid concentrations as low as 4 g/L, a sequencing batch culture (SBC) with a C/N of 100 increased biomass production to 4.21 g/L, with a lipid content of 38.6%. These results provide usable culture strategies for lipid production by R. toruloides AS 2.1389 when using diverse waste-derived volatile fatty acids.
authors: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center of Advanced Technology and Equipment for Water Pollution Control, Tongji University, Shanghai 200092, China.
authors: Xiang-Feng
authors: XF
authors: Huang
authors: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center of Advanced Technology and Equipment for Water Pollution Control, Tongji University, Shanghai 200092, China.
authors: Jia-Nan
authors: JN
authors: Liu
authors: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center of Advanced Technology and Equipment for Water Pollution Control, Tongji University, Shanghai 200092, China.
authors: Li-Jun
authors: LJ
authors: Lu
authors: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center of Advanced Technology and Equipment for Water Pollution Control, Tongji University, Shanghai 200092, China.
authors: Kai-Ming
authors: KM
authors: Peng
authors: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center of Advanced Technology and Equipment for Water Pollution Control, Tongji University, Shanghai 200092, China.
authors: Gao-Xiang
authors: GX
authors: Yang
authors: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center of Advanced Technology and Equipment for Water Pollution Control, Tongji University, Shanghai 200092, China. Electronic address: liujia@tongji.edu.cn.
authors: Jia
authors: J
authors: Liu
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D001487:Basidiomycota / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D008055:Lipids; D002244:Carbon; D009584:Nitrogen; D019342:Acetic Acid
keywords: Acetic acid; Culture strategies; Microbial oils; Rhodosporidium toruloides