title: Differentiation of UK endemic strains of Salmonella enterica serovar Newport from epidemic North American strains by PCR detection of a truncated bapA chromosomal gene.
abstract: The aim of this study was to develop a PCR test to detect chromosomal differences between epidemic multidrug resistant (epi-MDR) strains of Salmonella enterica serovar Newport (S. Newport) and non-epi-MDR strains of S. Newport that are endemic to the United Kingdom (UK). Sequence analysis of the biofilm-associated protein A gene (bapA) showed that epi-MDR strains of S. Newport from the United States of America (USA) had a deletion of 309 bp, which was not present in non-epi-MDR strains of S. Newport from the UK. A PCR test was developed using primers designed to target this difference and was applied to a panel of S. Newport isolates comprising of strains from the UK (n=20, non-epi-MDR), from the USA (n=10, epi-MDR) and from Canada (n=7). A second panel of isolates (n=73) was used to assess the test specificity, and these isolates consisted of non-Newport Salmonella serovars (n=25), and other epidemic serovars (n=48). Epi-MDR S. Newport isolates produced a characteristic 505 bp amplicon, whereas non-epi-MDR S. Newport isolates produced an 814 bp amplicon. The bapA PCR has potential to discriminate between these S. Newport strains irrespective of their carrying resistance genes.
authors: Animal and Plant Health Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom. Electronic address: robert.horton@apha.gsi.gov.uk.
authors: R A
authors: RA
authors: Horton
authors: Animal and Plant Health Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors: R
authors: R
authors: Card
authors: Animal and Plant Health Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
authors: L P
authors: LP
authors: Randall
authors: Animal and Plant Health Agency (Shrewsbury), Kendal Road, Harlescott, Shrewsbury SY1 4HD, United Kingdom.
authors: C J
authors: CJ
authors: Teale
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002170:Canada; D024901:Drug Resistance, Multiple, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics*; D017422:Sequence Analysis, DNA; D006113:United Kingdom; D014481:United States
chemical_list: D000900:Anti-Bacterial Agents
keywords: S. Newport; Strain differentiation; bapA gene