title: Exploring Nonspecific Protein Adsorption on Lignocellulosic Amphiphilic Bicomponent Films.
abstract: In this contribution, we explore the interaction of lignocellulosics and proteins aiming at a better understanding of their synergistic role in natural systems. In particular, the manufacturing and characterization of amphiphilic bicomponent thin films composed of hydrophilic cellulose and a hydrophobic lignin ester in different ratios is presented which may act as a very simplified model for real systems. Besides detailed characterizations of the films and mechanisms to explain their formation, nonspecific protein adsorption using bovine serum albumin (BSA) onto the films was studied using a quartz crystal microbalance with dissipation (QCM-D). As it turns out, the rather low nonspecific protein adsorption of BSA on cellulose is further reduced when these hydrophobic lignins are incorporated into the films. The lignin ester acts in these blend films as sacrificial component, probably via an emulsification mechanism. Additionally, the amphiphilicity of the films may prevent the adsorption of BSA as well. Although there are some indications, it remains unclear whether any kind of protein interactions in such systems are of specific nature.
authors: Institute for Chemistry and Technology of Materials, Graz University of Technology , Stremayrgasse 9, 8010 Graz, Austria.
authors: Simone
authors: S
authors: Strasser
authors: Institute for Chemistry and Technology of Materials, Graz University of Technology , Stremayrgasse 9, 8010 Graz, Austria.
authors: Katrin
authors: K
authors: Niegelhell
authors: Institute for Chemistry and Technology of Materials, Graz University of Technology , Stremayrgasse 9, 8010 Graz, Austria.
authors: Manuel
authors: M
authors: Kaschowitz
authors: Institute of Engineering and Design of Materials, University of Maribor , Smetanova 17, 2000 Maribor, Slovenia.
authors: Sabina
authors: S
authors: Markus
authors: Institute for Chemistry and Technology of Materials, Graz University of Technology , Stremayrgasse 9, 8010 Graz, Austria.|Institute of Engineering and Design of Materials, University of Maribor , Smetanova 17, 2000 Maribor, Slovenia.
authors: Rupert
authors: R
authors: Kargl
authors: Institute of Engineering and Design of Materials, University of Maribor , Smetanova 17, 2000 Maribor, Slovenia.
authors: Karin
authors: K
authors: Stana-Kleinschek
authors: Institute for Chemistry and Technology of Materials, Graz University of Technology , Stremayrgasse 9, 8010 Graz, Austria.
authors: Christian
authors: C
authors: Slugovc
authors: Institute of Chemistry, University of Graz , Heinrichstrasse 28, 8010 Graz, Austria.
authors: Tamilselvan
authors: T
authors: Mohan
authors: Institute for Chemistry and Technology of Materials, Graz University of Technology , Stremayrgasse 9, 8010 Graz, Austria.|Institute of Engineering and Design of Materials, University of Maribor , Smetanova 17, 2000 Maribor, Slovenia.
authors: Stefan
authors: S
authors: Spirk
mesh_terms: D000327:Adsorption; D008031:Lignin / Q000737:chemistry*; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D014908:Triticum / Q000737:chemistry
chemical_list: D008567:Membranes, Artificial; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D008031:Lignin