title: miR-448 suppressed gastric cancer proliferation and invasion by regulating ADAM10.
abstract: MicroRNAs (miRNAs) are a class of short, noncoding RNAs that act a crucial role in tumor development. Previous studies showed that miR-448 expression was deregulated in many tumors. However, the role of miR-448 in gastric cancer (GC) remains unknown. In our study, we demonstrated that miR-448 expression was downregulated in GC tissues compared with the corresponding nontumor tissues. We also showed that miR-448 expression was downregulated in GC cell lines. Ectopic expression of miR-448 suppressed GC cell proliferation, colony formation, and invasion. Moreover, we identified A Disintegrin And Metalloproteinases 10 (ADAM10) as a direct target gene of miR-448 in GC cell. ADAM10 expression was upregulated in GC tissues and cells. Furthermore, the expression level of miR-448 was negatively correlated with the expression level of ADAM10 in GC tissues. Moreover, ADAM10 overexpression rescued the effect of miR-448-mediated GC cell proliferation, colony formation, and invasion. These results demonstrated that miR-448 might play as a tumor suppressor miRNA partly through targeting ADAM10 expression.
authors: Department of Gastrointenstinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China.
authors: Xuesong
authors: X
authors: Wu
authors: Department of Gastrointenstinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China.
authors: Haoran
authors: H
authors: Tang
authors: Department of Gastroenterology, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China.
authors: Guobin
authors: G
authors: Liu
authors: Department of Gastroenterology, The Affiliated YanAn Hospital of Kunming Medical University, Kunming, 650051, Yunnan, China.
authors: Hui
authors: H
authors: Wang
authors: Department of Gastrointenstinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China.
authors: Jie
authors: J
authors: Shu
authors: Department of Gastrointenstinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan, China. SunFeng1971@163.com.
authors: Feng
authors: F
authors: Sun
mesh_terms: D000072197:ADAM10 Protein / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D053829:Amyloid Precursor Protein Secretases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002455:Cell Division; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012313:RNA / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014410:Tumor Stem Cell Assay
chemical_list: C557400:MIRN448 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C039919:RNA, recombinant; D012313:RNA; D053829:Amyloid Precursor Protein Secretases; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human
keywords: ADAM10; Gastric cancer; miR-448; microRNAs