title: De novo transcriptome sequencing and gene expression profiling of spinach (Spinacia oleracea L.) leaves under heat stress.
abstract: Spinach (Spinacia oleracea) has cold tolerant but heat sensitive characteristics. The spinach variety 'Island,' is suitable for summer periods. There is lack molecular information available for spinach in response to heat stress. In this study, high throughput de novo transcriptome sequencing and gene expression analyses were carried out at different spinach variety 'Island' leaves (grown at 24 °C (control), exposed to 35 °C for 30 min (S1), and 5 h (S2)). A total of 133,200,898 clean reads were assembled into 59,413 unigenes (average size 1259.55 bp). 33,573 unigenes could match to public databases. The DEG of controls vs S1 was 986, the DEG of control vs S2 was 1741 and the DEG of S1 vs S2 was 1587. Gene Ontology (GO) and pathway enrichment analysis indicated that a great deal of heat-responsive genes and other stress-responsive genes were identified in these DEGs, suggesting that the heat stress may have induced an extensive abiotic stress effect. Comparative transcriptome analysis found 896 unique genes in spinach heat response transcript. The expression patterns of 13 selected genes were verified by RT-qPCR (quantitative real-time PCR). Our study found a series of candidate genes and pathways that may be related to heat resistance in spinach.
authors: Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.
authors: Jun
authors: J
authors: Yan
authors: Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.|Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.
authors: Li
authors: L
authors: Yu
authors: Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing, Jiangsu Province, 210014, China.
authors: Jiping
authors: J
authors: Xuan
authors: Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.|Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.
authors: Ying
authors: Y
authors: Lu
authors: Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.|Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.
authors: Shijun
authors: S
authors: Lu
authors: Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.|Horticulture Research Institute, Shanghai Academy Agricultural Sciences; Key Laboratory of Protected Horticulture Technology, Shanghai, 201403, China.
authors: Weimin
authors: W
authors: Zhu
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006358:Hot Temperature; D058977:Molecular Sequence Annotation; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D018724:Spinacia oleracea / Q000235:genetics* / Q000254:growth & development; D013312:Stress, Physiological