title: A Non-Synonymous Single Nucleotide Polymorphism in the HJURP Gene Associated with Susceptibility to Hepatocellular Carcinoma among Chinese.
abstract: OBJECTIVE
  HJURP (Holliday Junction-Recognizing Protein) plays dual roles in DNA repair and in accurate chromosome segregation during mitosis. We examined whether the single nucleotide polymorphisms (SNPs) of HJURP were associated with the risk of occurrence of hepatocellular carcinoma (HCC) among chronic hepatitis B virus (HBV) carriers from well-known high-risk regions for HCC in China.
  
  
  METHODS
  Twenty-four haplotype-tagging SNPs across HJURP were selected from HapMap data using the Haploview software. We genotyped these 24 SNPs using the using Sequenom's iPLEX assay in the Fusui population, consisting of 348 patients with HCC and 359 cancer-free controls, and further investigated the significantly associated SNP using the TaqMan assay in the Haimen population, consisting of 100 cases and 103 controls. The genetic associations with the risk of HCC were analyzed by logistic regression.
  
  
  RESULTS
  We observed an increased occurrence of HCC consistently associated with A/C or C/C genotypes of the non-synonymous SNP rs3771333 compared with the A/A genotype in both the Fusui and Haimen populations, with a pooled odds ratio 1.82 (95% confidence interval, 1.33-2.49; P = 1.9 × 10-4). Case-only analysis further indicated that carriers of the at-risk C allele were younger than those carrying the A/A genotype (P = 0.0016). In addition, the expression levels of HJURP in C allele carriers were lower than that in A/A genotype carriers (P = 0.0078 and 0.0010, for mRNA and protein levels, respectively).
  
  
  CONCLUSIONS
  Our findings suggest that rs3771333 in HJURP may play a role in mediating the susceptibility to HCC among Chinese.
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.|Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi, China.|The Third Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors: Wenfeng
authors: W
authors: Huang
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Hongxing
authors: H
authors: Zhang
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Yumin
authors: Y
authors: Hao
authors: Department of Gastroenterology and Hepatology, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, Nanjing, Jiangsu, China.
authors: Xiaobing
authors: X
authors: Xu
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Yun
authors: Y
authors: Zhai
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing, China.
authors: Shaoxia
authors: S
authors: Wang
authors: Department of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing, China.
authors: Yang
authors: Y
authors: Li
authors: The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors: Fuchao
authors: F
authors: Ma
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Yuanfeng
authors: Y
authors: Li
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Zhifu
authors: Z
authors: Wang
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Yang
authors: Y
authors: Zhang
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Xiumei
authors: X
authors: Zhang
authors: Liver Cancer Institute at Fusui County, Guangxi, China.
authors: Renxiang
authors: R
authors: Liang
authors: Liver Cancer Institute at Fusui County, Guangxi, China.
authors: Zhongliang
authors: Z
authors: Wei
authors: Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors: Ying
authors: Y
authors: Cui
authors: Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors: Yongqiang
authors: Y
authors: Li
authors: Disease Prevention and Control Center at Haimen County, Jiangsu, China.
authors: Xinsen
authors: X
authors: Yu
authors: Department of Gastroenterology and Hepatology, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, Nanjing, Jiangsu, China.
authors: Hongzan
authors: H
authors: Ji
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Fuchu
authors: F
authors: He
authors: Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors: Weimin
authors: W
authors: Xie
authors: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, China.|National Engineering Research Center for Protein Drugs, Beijing, China.|National Center for Protein Sciences Beijing, Beijing, China.
authors: Gangqiao
authors: G
authors: Zhou
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D016022:Case-Control Studies; D015897:Comorbidity; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D015810:Linkage Disequilibrium; D008113:Liver Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000821:virology; D020641:Polymorphism, Single Nucleotide
chemical_list: D004268:DNA-Binding Proteins; C524371:HJURP protein, human