title: A rapid and sensitive LC-MS/MS method for quantification of quercetin-3-O-β-d-glucopyranosyl-7-O-β-d-gentiobioside in plasma and its application to a pharmacokinetic study.
abstract: A rapid and sensitive LC-MS/MS method with good accuracy and precision was developed and validated for the pharmacokinetic study of quercetin-3-O-β-d-glucopyranosyl-7-O-β-d-gentiobioside (QGG) in Sprague-Dawley rats. Plasma samples were simply precipitated by methanol and then analyzed by LC-MS/MS. A Venusil® ASB C18 column (2.1 × 50 mm, i.d. 5 μm) was used for separation, with methanol-water (50:50, v/v) as the mobile phase at a flow rate of 300 μL/min. The optimized mass transition ion-pairs (m/z) for quantitation were 787.3/301.3 for QGG, and 725.3/293.3 for internal standard. The linear range was 7.32-1830 ng/mL with an average correlation coefficient of 0.9992, and the limit of quantification was 7.32 ng/mL. The intra- and inter-day precision and accuracy were less than ±15%. At low, medium and high quality control concentrations, the recovery and matrix effect of the analyte and IS were in the range of 89.06-92.43 and 88.58-97.62%, respectively. The method was applied for the pharmacokinetic study of QGG in Sprague-Dawley rats. Copyright © 2016 John Wiley & Sons, Ltd.
authors: Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renmin Street, Jinzhou, 121001, China.
authors: Xin
authors: X
authors: He
authors: Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renmin Street, Jinzhou, 121001, China.
authors: Guizhou
authors: G
authors: Tao
authors: Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renmin Street, Jinzhou, 121001, China.
authors: Hang
authors: H
authors: Gao
authors: Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renmin Street, Jinzhou, 121001, China.
authors: Keyan
authors: K
authors: Li
authors: Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renmin Street, Jinzhou, 121001, China.
authors: Yazhuo
authors: Y
authors: Zhang
authors: Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renmin Street, Jinzhou, 121001, China.
authors: Limin
authors: L
authors: Sun
authors: Department of Cardiology, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renmin Street, Jinzhou, 121001, China.
authors: Yingjie
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005960:Glucosides; C500372:quercetin-3-O-glucopyranosyl-7-O-gentiobioside; D011794:Quercetin
keywords: LC-MS/MS; pharmacokinetic study; quercetin-3-O-β-d-glucopyranosyl-7-O-β-d- gentiobioside; rat plasma