title: Surface bound VEGF mimicking peptide maintains endothelial cell proliferation in the absence of soluble VEGF in vitro.
abstract: Continuous glucose monitoring is an efficient method for the management of diabetes and in limiting the complications induced by large fluctuations in glucose levels. For this, intravascular systems may assist in producing more reliable and accurate devices. However, neovascularization is a key factor to be addressed in improving their biocompatibility. In this scope, the perennial modification of the surface of an implant with the proangiogenic Vascular Endothelial Growth Factor mimic peptide (SVVYGLR peptide sequence) holds great promise. Herein, we report on the preparation of gold substrates presenting the covalently grafted SVVYGLR peptide sequence and their effect on HUVEC behavior. Effective coupling was demonstrated using XPS and PM-IRRAS. The produced surfaces were shown to be beneficial for HUVEC adhesion. Importantly, surface bound SVVYGLR is able to maintain HUVEC proliferation even in the absence of soluble VEGF. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1425-1436, 2016.
authors: University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600, France.
authors: Guillaume
authors: G
authors: Le Saux
authors: University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600, France.
authors: Laurent
authors: L
authors: Plawinski
authors: Equipe Labellisée Contre Le Cancer, F-33607, Pessac, France.|INSERM, U869, ARNA Laboratory, Equipe Labellisée Contre Le Cancer, Bordeaux, F-33076, France.
authors: Camila
authors: C
authors: Parrot
authors: University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600, France.
authors: Sylvain
authors: S
authors: Nlate
authors: University of Bordeaux, ISM, UMR 5255, Talence, F-33400, France.
authors: Laurent
authors: L
authors: Servant
authors: Equipe Labellisée Contre Le Cancer, F-33607, Pessac, France.|INSERM, U869, ARNA Laboratory, Equipe Labellisée Contre Le Cancer, Bordeaux, F-33076, France.
authors: Martin
authors: M
authors: Teichmann
authors: University of Bordeaux, ISM, UMR 5255, Talence, F-33400, France.
authors: Thierry
authors: T
authors: Buffeteau
authors: University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600, France.
authors: Marie-Christine
authors: MC
authors: Durrieu
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D056951:Photoelectron Spectroscopy; D012995:Solubility; D013499:Surface Properties; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D010455:Peptides; D042461:Vascular Endothelial Growth Factor A; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: SVVYGLR peptide; bioactivity; endothelial cell; growth factors; surface modification