title: Imparting commercial antimicrobial dressings with low-adherence to burn wounds.
abstract: The objective of our study was to decrease the wound adherence of commercial silver based wound dressings by depositing a non-adherent layer. Our hypothesis was that this non-adherent layer will lower the dressing's adherence to burn wounds without compromising the antimicrobial activity or increasing the cytotoxicity. A polyacrylamide (PAM) hydrogel layer was grafted on two commercial silver antimicrobial dressings (silver nanocrystal dressing (NC) and silver plated dressing (SP)) using a proprietary technique. The grafted PAM served as the non-adherent layer. Dressing adherence was measured with a previously published in vitro gelatin model using an Instron mechanical force testing instrument. The dressings were challenged with two clinically retrieved bacterial strains (Methicillin-resistant Staphylococcus aureus (MRSA) and multidrug resistant (MDR) Pseudomonas aeruginosa) with both a disk diffusion test, and a suspension antibacterial test. The cytotoxicity of samples to human neonatal fibroblast cells was evaluated with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. Both untreated dressings showed high peeling energy: 2070±453J/m(2) (NC) and 669±68J/m(2) (SP), that decreased to 158±119J/m(2) (NC) and 155±138J/m(2) (SP) with the PAM deposition. Addition of the PAM caused no significant difference in zone of inhibition (ZOI) (disk diffusion test) or antibacterial kinetics (suspension test) against both bacteria (p>0.05, n=6) in either dressing. Survival of fibroblasts was improved by the PAM grafting from 48±5% to 60±3% viable cells in the case of NC and from 55±8% to 61±4% viable cells in SP (p<0.05, n=12). It was concluded that PAM as a non-adherent layer significantly decreases the adherence of these two commercial antimicrobial dressings in an in vitro gelatin model while preserving their antimicrobial efficacy, and reducing their cytotoxicity.
authors: Department of Biosystems Engineering, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, Canada.
authors: Sogol
authors: S
authors: Asghari
authors: Manitoba Firefighters Burn Unit, Department of Surgery, Faculty of Medicine, University of Manitoba, Winnipeg, MB, Canada.
authors: Sarvesh
authors: S
authors: Logsetty
authors: Department of Biosystems Engineering, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB, Canada; Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada; Department of Chemistry, University of Manitoba, Winnipeg, MB, Canada. Electronic address: Song.Liu@umanitoba.ca.
authors: Song
authors: S
authors: Liu
mesh_terms: D000180:Acrylic Resins / Q000627:therapeutic use; D000890:Anti-Infective Agents / Q000032:analysis / Q000494:pharmacology; D047070:Bandages, Hydrocolloid; D002056:Burns / Q000628:therapy*; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000032:analysis / Q000494:pharmacology; D014946:Wound Infection / Q000517:prevention & control*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000890:Anti-Infective Agents; D012834:Silver; D000099166:Polyacrylamides
keywords: Burn wound dressing; Hydrogel; Non-adherent dressing; Polyacrylamide; Silver dressing