title: Human VE-Cadherin Fusion Protein as an Artificial Extracellular Matrix Enhancing the Proliferation and Differentiation Functions of Endothelial Cell.
abstract: In an attempt to enhance endothelial cell capture and promote the vascularization of engineered tissue, we biosynthesized and characterized the recombinant fusion protein consisting of human vascular endothelial-cadherin extracellular domain and immunoglobulin IgG Fc region (hVE-cad-Fc) to serve as a bioartificial extracellular matrix. The hVE-cad-Fc protein naturally formed homodimers and was used to construct hVE-cad-Fc matrix by stably adsorbing on polystyrene plates. Atomic force microscop assay showed uniform hVE-cad-Fc distribution with nanorod topography. The hVE-cad-Fc matrix markedly promoted human umbilical vein endothelial cells (HUVECs) adhesion and proliferation with fibroblastoid morphology. Additionally, the hVE-cad-Fc matrix improved HUVECs migration, vWF expression, and NO release, which are closely related to vascularization. Furthermore, the hVE-cad-Fc matrix activated endogenous VE-cadherin/β-catenin proteins and effectively triggered the intracellular signals such as F-actin stress fiber, p-FAK, AKT, and Bcl-2. Taken together, hVE-cad-Fc could be a promising bioartificial matrix to promote vascularization in tissue engineering.
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Ke
authors: K
authors: Xu
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Qizhi
authors: Q
authors: Shuai
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Xiaoning
authors: X
authors: Li
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Yan
authors: Y
authors: Zhang
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Chao
authors: C
authors: Gao
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Lei
authors: L
authors: Cao
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Feifei
authors: F
authors: Hu
authors: Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science , Tsukuba, Japan.
authors: Toshihiro
authors: T
authors: Akaike
authors: National Engineering Research Center for Biomaterials, Sichuan University , Chengdu, 610064, China.
authors: Jian-xi
authors: JX
authors: Wang
authors: National Engineering Research Center for Biomaterials, Sichuan University , Chengdu, 610064, China.
authors: Zhongwei
authors: Z
authors: Gu
authors: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University , Tianjin, 300071, China.
authors: Jun
authors: J
authors: Yang
mesh_terms: D015820:Cadherins / Q000235:genetics / Q000494:pharmacology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry
chemical_list: D015820:Cadherins; D007141:Immunoglobulin Fc Fragments; D011994:Recombinant Proteins