title: Microfluidic Device to Quantify the Behavior of Therapeutic Bacteria in Three-Dimensional Tumor Tissue.
abstract: Microfluidic devices enable precise quantification of the interactions between anti-cancer bacteria and tumor tissue. Direct observation of bacterial movement and gene expression in tissue is difficult with either monolayers of cells or tumor-bearing mice. Quantification of these interactions is necessary to understand the inherent mechanisms of bacterial targeting and to develop modified organisms with enhanced therapeutic properties. Here we describe the procedures for designing, printing, and assembling microfluidic tumor-on-a-chip devices. We also describe the procedures for inserting three-dimensional tumor-cell masses, exposure to bacteria, and analyzing the resultant images.
authors: Department of Chemical Engineering, University of Massachusetts, Amherst, 159 Goessmann Laboratory, 686 North Pleasant Street, Amherst, MA, 01003-9303, USA.
authors: Emily L
authors: EL
authors: Brackett
authors: Department of Chemical Engineering, University of Massachusetts, Amherst, 159 Goessmann Laboratory, 686 North Pleasant Street, Amherst, MA, 01003-9303, USA.
authors: Charles A
authors: CA
authors: Swofford
authors: Department of Chemical Engineering, University of Massachusetts, Amherst, 159 Goessmann Laboratory, 686 North Pleasant Street, Amherst, MA, 01003-9303, USA. forbes@ecs.umass.edu.
authors: Neil S
authors: NS
authors: Forbes
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D056656:Lab-On-A-Chip Devices; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D018874:Spheroids, Cellular; D059008:Time-Lapse Imaging; D046509:Tissue Culture Techniques; D014407:Tumor Cells, Cultured
keywords: Accumulation; Cancer; Lithography; Microfluidic device; Motility; PDMS; Penetration; Tumor-on-a-chip; Tumor-targeting bacteria