title: Exploring the Leaf Beetle Fauna (Coleoptera: Chrysomelidae) of an Ecuadorian Mountain Forest Using DNA Barcoding.
abstract: BACKGROUND
  Tropical mountain forests are hotspots of biodiversity hosting a huge but little known diversity of insects that is endangered by habitat destruction and climate change. Therefore, rapid assessment approaches of insect diversity are urgently needed to complement slower traditional taxonomic approaches. We empirically compare different DNA-based species delimitation approaches for a rapid biodiversity assessment of hyperdiverse leaf beetle assemblages along an elevational gradient in southern Ecuador and explore their effect on species richness estimates.
  
  
  RESULTS
  Based on a COI barcode data set of 674 leaf beetle specimens (Coleoptera: Chrysomelidae) of 266 morphospecies from three sample sites in the Podocarpus National Park, we employed statistical parsimony analysis, distance-based clustering, GMYC- and PTP-modelling to delimit species-like units and compared them to morphology-based (parataxonomic) species identifications. The four different approaches for DNA-based species delimitation revealed highly similar numbers of molecular operational taxonomic units (MOTUs) (n = 284-289). Estimated total species richness was considerably higher than the sampled amount, 414 for morphospecies (Chao2) and 469-481 for the different MOTU types. Assemblages at different elevational levels (1000 vs. 2000 m) had similar species numbers but a very distinct species composition for all delimitation methods. Most species were found only at one elevation while this turnover pattern was even more pronounced for DNA-based delimitation.
  
  
  CONCLUSIONS
  Given the high congruence of DNA-based delimitation results, probably due to the sampling structure, our study suggests that when applied to species communities on a regionally limited level with high amount of rare species (i.e. ~50% singletons), the choice of species delimitation method can be of minor relevance for assessing species numbers and turnover in tropical insect communities. Therefore, DNA-based species delimitation is confirmed as a valuable tool for evaluating biodiversity of hyperdiverse insect communities, especially when exact taxonomic identifications are missing.
authors: Zoological Research Museum Alexander Koenig, Bonn, Germany.
authors: Birthe
authors: B
authors: Thormann
authors: Zoological Research Museum Alexander Koenig, Bonn, Germany.
authors: Dirk
authors: D
authors: Ahrens
authors: Museo de Colecciones Biológicas MUTPL, Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, Loja, Ecuador.
authors: Diego
authors: D
authors: Marín Armijos
authors: Department of Animal Ecology and Tropical Biology, Biocenter, University of Würzburg, Würzburg, Germany.
authors: Marcell K
authors: MK
authors: Peters
authors: Abteilung Biologie, Institut für Integrierte Naturwissenschaften, Universität Koblenz-Landau, Koblenz, Germany.
authors: Thomas
authors: T
authors: Wagner
authors: Zoological Research Museum Alexander Koenig, Bonn, Germany.
authors: Johann W
authors: JW
authors: Wägele
mesh_terms: D000818:Animals; D044822:Biodiversity; D001517:Coleoptera / Q000145:classification* / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D004484:Ecuador; D065928:Forests