title: Cell differentiation and the multiple drug resistance phenotype in human erythroleukemic cells.
abstract: The gene expression of Oct-4, a transcription factor and hematopoietic stem cell marker, is higher in Lucena lines, which is MDR, and the gene Alox-5 has also been implicated in the differentiation of some cell lines. The aim of this study was to compare the response to PMA-induced differentiation in MDR and non-MDR cells. We observed the differentiation to megakaryocytes in the K562 cell line, which is non-MDR. The expression of Alox-5 and Nanog genes was downregulated and that of Mdr-1 was upregulated in K562 cells. The Lucena cell line contained a higher number of megakaryocytes than the non-MDR, but this number was not altered by PMA, as well as Mdr-1 gene expression. However, Alox-5 expression was downregulated. Alox-5, Mdr-1, Nanog, Oct-4 and Sox-2 basal expression was also evaluated in the K562, Lucena and FEPS (also MDR) cell lines. The transcription factors gene expression was similar in MDR cell lines. The expression of Alox-5 was higher in the non-MDR cell line, while FEPS had the lowest expression of this gene. The opposite pattern was observed for Mdr-1 gene expression. These results suggest that the Alox-5 gene might play a role in the differentiation of these cell lines.
authors: Programa de Pós-graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, Universidade Federal do Rio Grande-FURG, Rio Grande, RS, Brazil; Laboratório de Cultura Celular, Instituto de Ciências Biológicas, FURG, Rio Grande, RS, Brazil.
authors: Michele
authors: M
authors: Carrett-Dias
authors: Laboratório de Cultura Celular, Instituto de Ciências Biológicas, FURG, Rio Grande, RS, Brazil.
authors: Leda Karine
authors: LK
authors: Almeida
authors: Laboratório de Patologia, Faculdade de Medicina (FaMed), FURG, Rio Grande, RS, Brazil.
authors: Juliano Lacava
authors: JL
authors: Pereira
authors: Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, FURG, Rio Grande, RS, Brazil.
authors: Daniela Volcan
authors: DV
authors: Almeida
authors: Laboratório de Cultura Celular, Instituto de Ciências Biológicas, FURG, Rio Grande, RS, Brazil.
authors: Daza Moraes Vaz Batista
authors: DM
authors: Filgueira
authors: Programa de Pós-graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, Universidade Federal do Rio Grande-FURG, Rio Grande, RS, Brazil; Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, FURG, Rio Grande, RS, Brazil.
authors: Luis Fernando
authors: LF
authors: Marins
authors: Programa de Pós-graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, Universidade Federal do Rio Grande-FURG, Rio Grande, RS, Brazil; Laboratório de Cultura Celular, Instituto de Ciências Biológicas, FURG, Rio Grande, RS, Brazil. Electronic address: anavotto@furg.br.
authors: Ana Paula de Souza
authors: AP
authors: Votto
authors: Programa de Pós-graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, Universidade Federal do Rio Grande-FURG, Rio Grande, RS, Brazil; Laboratório de Cultura Celular, Instituto de Ciências Biológicas, FURG, Rio Grande, RS, Brazil.
authors: Gilma Santos
authors: GS
authors: Trindade
mesh_terms: D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D001094:Arachidonate 5-Lipoxygenase; C583212:ALOX5 protein, human
keywords: Alox-5; Differentiation; Leukemia; MDR; PMA; Tumoral stem cell