title: Isolation and Characterization of a Novel Dicistrovirus Associated with Moralities of the Great Freshwater Prawn, Macrobrachium rosenbergii.
abstract: The giant freshwater prawn, Macrobrachium rosenbergii, is an economically important crustacean and is farmed in many countries. Since 2009, a larval mortality syndrome of M. rosenbergii has broken out and spread widely in the main breeding area, including Zhejiang, Jiangsu, Guangxi, and Guangdong Provinces in mainland China. A novel virus, named Macrobrachium rosenbergii Taihu virus (MrTV), was isolated from the moribund larvae and was determined to be the causative agent of the M. rosenbergii larval mortality syndrome by experimental infection. Further genomic sequencing suggested that the MrTV genome is monopartite, 10,303 nt in length, and dicistronic with two non-overlapping open reading frames (ORFs) separated by an intergenic region (IGR) and flanked by untranslated regions (UTRs). Phylogenetic analysis using the full-length genomic sequence and the putative amino acid sequences of the capsid protein revealed that MrTV was more closely related to the taura syndrome virus (TSV) than to any other viruses. According to these molecular features, we proposed that MrTV is a new species in the genus Aparavirus, family Dicistroviridae. These results may shed light on controlling larval mortality syndrome in M. rosenbergii.
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. panxiaoyi@163.com.
authors: Xiaoyi
authors: X
authors: Pan
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. cz0305@163.com.
authors: Zheng
authors: Z
authors: Cao
authors: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. jfyuan@mail.hzau.edu.cn.
authors: Junfa
authors: J
authors: Yuan
authors: State key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan 430071, China. zlshi@wh.iov.cn.
authors: Zhengli
authors: Z
authors: Shi
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. yuanxuemei_2008@163.com.
authors: Xuemei
authors: X
authors: Yuan
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. linly0531@163.com.
authors: Lingyun
authors: L
authors: Lin
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. fionxu126@163.com.
authors: Yang
authors: Y
authors: Xu
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. yjy0913lxl@126.com.
authors: Jiayun
authors: J
authors: Yao
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. 13867253205@163.com.
authors: Guijie
authors: G
authors: Hao
authors: Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. sjinyu@126.com.
authors: Jinyu
authors: J
authors: Shen
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D021901:DNA, Intergenic; D016679:Genome, Viral; D016366:Open Reading Frames; D033541:Palaemonidae / Q000821:virology*; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D036022:Capsid Proteins; D021901:DNA, Intergenic
keywords: Aparavirus; Macrobrachium rosenbergii; dicistrovirus; larval mortality syndrome; taihu virus