title: Efficacy of different "in-office" desensitizing treatment methods: An in vitro SEM analysis.
abstract: OBJECTIVE
  To evaluate, in vitro, the obliteration of dentin tubules promoted by different desensitizing methods, before and after pH cycling.
  
  
  METHODS
  Human dentin blocks of 4x4 mm were randomly divided into: control group (n = 20): no treatment; Group GH (n = 20): surface treatment with a solution containing glutaraldehyde (5.1%), HEMA (36.1%), sodium fluoride (NaF), and deionized water; Group NP (n = 20): surface treatment with a calcium phosphate gel containing nanostructured hydroxyapatite crystals, NaF and NK; and Group ARG (n = 20): surface treatment with a paste containing CaCO3 and 8% arginine. After treatment, 10 samples of each group were evaluated in scanning electron microscopy (SEM) while another 10 were included in a pH cycling procedure for 48 hours, and then analyzed in the SEM. All SEM images were evaluated by three calibrated examiners regarding dentin tubular obliteration. Mann-Whitney and Kruskal-Wallis tests were used for data analysis (P < 0.05).
  
  
  RESULTS
  The GH, NP, and ARG groups promoted immediate obliteration higher than the control group (P < 0.05) with NP and ARG groups superior to GH group (P < 0.05). After pH cycling, an increase in tubular obliteration in the GH group was observed, which was similar to the NP and ARG groups (P > 0.05), even though all groups promoted higher obliteration than the control group (P < 0.05). All treatments were effective in a tubular obliteration.
authors: Renata De Cássia Gomes
authors: Rde C
authors: Camacho
authors: Giovana Lecio
authors: GL
authors: Miranda
authors: Fabio Tonasso
authors: FT
authors: Oliveira
authors: Fernanda Vieira
authors: FV
authors: Ribeiro
authors: Suzana Peres
authors: SP
authors: Pimentel
authors: Renato Corrêa Viana
authors: RC
authors: Casarin
mesh_terms: D001120:Arginine / Q000494:pharmacology; D002119:Calcium Carbonate / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D057133:Dentin Desensitizing Agents / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008689:Methacrylates / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010710:Phosphates / Q000494:pharmacology; D011897:Random Allocation; D012969:Sodium Fluoride / Q000494:pharmacology; D013052:Spectrometry, X-Ray Emission; D013997:Time Factors
chemical_list: D002130:Calcium Phosphates; D057133:Dentin Desensitizing Agents; D008689:Methacrylates; D010710:Phosphates; C585805:pro-argin; C005044:hydroxyethyl methacrylate; D012969:Sodium Fluoride; D017886:Durapatite; D001120:Arginine; C020243:calcium phosphate; D002119:Calcium Carbonate; D005459:Fluorides; D005976:Glutaral