title: Equilibria Between Cell Membranes and Electrolyte Solution: Effect of Fatal Accidental Hypothermia.
abstract: Equilibria between the membranes of erythrocytes as well as thrombocytes and solution ions in fatal accidental hypothermia were analyzed using a theoretical four-equilibria model. The model was developed to determinate parameters characterizing cell membrane-surrounding ion interactions: the total surface concentrations of both acidic and basic groups C A, C B, and association constants K AH, K BOH. Knowledge of these parameters was necessary to calculate the theoretical values of surface charge density. The model was validated by curve-fitting the experimental data points to simulated data generated by the model. The experimental and theoretical surface charge density values agree at pH 2-8, at higher pH the deviation was observed.
authors: Institute of Chemistry, University in Bialystok, K. Ciolkowskiego 1K, 15-245, Białystok, Poland. aneta@uwb.edu.pl.
authors: Aneta D
authors: AD
authors: Petelska
authors: Institute of Chemistry, University in Bialystok, K. Ciolkowskiego 1K, 15-245, Białystok, Poland.
authors: Joanna
authors: J
authors: Kotyńska
authors: Institute of Chemistry, University in Bialystok, K. Ciolkowskiego 1K, 15-245, Białystok, Poland.
authors: Monika
authors: M
authors: Naumowicz
authors: Institute of Chemistry, University in Bialystok, K. Ciolkowskiego 1K, 15-245, Białystok, Poland.
authors: Zbigniew A
authors: ZA
authors: Figaszewski
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D001344:Autopsy; D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004573:Electrolytes / Q000737:chemistry / Q000378:metabolism*; D055724:Electrophysiological Phenomena; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D007035:Hypothermia / Q000378:metabolism* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D008962:Models, Theoretical; D012996:Solutions; D055815:Young Adult
chemical_list: D004573:Electrolytes; D012996:Solutions
keywords: Acid–base equilibria; Erythrocytes; Fatal accidental hypothermia; Microelectrophoresis; Surface charge density; Thrombocytes