title: Identification of 14-3-3zeta associated protein networks in oral cancer.
abstract: Advancements in genomics, proteomics, and bioinformatics have improved our understanding of gene/protein networks involved in intra- and intercellular communication and tumor-host interactions. Using proteomics integrated with bioinformatics, previously we reported overexpression of 14-3-3ζ in premalignant oral lesions and oral squamous cell carcinoma tissues in comparison with normal oral epithelium. 14-3-3ζ emerged as a novel molecular target for therapeutics and a potential prognostic marker in oral squamous cell carcinoma patients. However, the role of 14-3-3ζ in development and progression of oral cancer is not known yet. This study aimed to identify the 14-3-3ζ associated protein networks in oral cancer cell lines using IP-MS/MS and bioinformatics. A total of 287 binding partners of 14-3-3ζ were identified in metastatic (MDA1986) and nonmetastatic (SCC4) oral cancer cell lines including other 14-3-3 isoforms (2%), proteins involved in apoptosis (2%), cytoskeleton (9%), metabolism (16%), and maintenance of redox potential (2%). Our bioinformatics analysis revealed involvement of 14-3-3ζ in protein networks regulating cell cycle, proliferation, apoptosis, cellular trafficking, and endocytosis in oral cancer. In conclusion, our data revealed several novel protein interaction networks involving 14-3-3ζ in oral cancer progression and metastasis.
authors: Department of Chemistry, Centre for Research in Mass Spectrometry, York University, Toronto, ON, Canada.
authors: Ajay
authors: A
authors: Matta
authors: Department of Chemistry, Centre for Research in Mass Spectrometry, York University, Toronto, ON, Canada.
authors: Olena
authors: O
authors: Masui
authors: Department of Chemistry, Centre for Research in Mass Spectrometry, York University, Toronto, ON, Canada.
authors: K W Michael
authors: KW
authors: Siu
authors: Department of Chemistry, Centre for Research in Mass Spectrometry, York University, Toronto, ON, Canada.|Department of Otolaryngology-Head and Neck Surgery, Joseph and Mildred Sonshine Family Centre for Head and Neck Diseases, Mount Sinai Hospital, Toronto, ON, Canada.|Alex and Simona Shnaider Research Laboratory in Molecular Oncology, Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada.|Department of Otolaryngology-Head and Neck Surgery, University of Toronto, Toronto, ON, Canada.
authors: Ranju
authors: R
authors: Ralhan
mesh_terms: D048948:14-3-3 Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D011485:Protein Binding; D060066:Protein Interaction Maps / Q000502:physiology*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D048948:14-3-3 Proteins; D020543:Proteome
keywords: 14-3-3 zeta; Cell biology; Cytoskeleton; Head and neck cancer; Metastasis; Protein networks