title: Apoptosis-mediated antiproliferative activity of friedolanostane triterpenoid isolated from the leaves ofGarcinia celebicaagainst MCF-7 human breast cancer cell lines.
abstract: The leaves of Garcinia celebica strongly inhibit the proliferation of MCF-7 human breast adenocarcinoma cell lines. The present study focused on investigating the active anticancer and antiproliferative compound from the G. celebica leaves and assessing its mechanism of action. Ethanol extracts of G. celebica were fractionated based on their polarity usingn-hexane, ethyl acetate and water. The antiproliferative properties were testedin vitroagainst MCF-7 human breast cancer cell lines using the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. The active compound was subsequently isolated using column chromatography and identified by nuclear magnetic resonance. The characterized compound was also tested for its antiproliferative properties and the mechanism by which it induces apoptosis in MCF-7 cells by western blot analysis of the activated apoptotic proteins. This resulted in the isolation of a friedolanostane triterpenoid, which was determined to be methyl-3α, 23-dihydroxy-17,14-friedolanstan-8,14,24-trien-26-oat. This compound inhibited MCF-7 cell proliferation in a time- and dose-dependent manner with IC50values of 82 and 70 µM for the 24 and 48 h treatments, respectively. Furthermore, the western blot analysis suggested that the compound exerted its anticancer activities by promoting apoptosis through the inhibition of the oncogenic protein Akt, thereby increasing the expression of poly (ADP-ribose) polymerase (PARP) protein. These results suggest that methyl-3α,23-dihydroxy-17,14-friedolanstan-8,14,24-trien-26-oat is the anticancer compound found in G. celebica, providing a basis for its potential use in cancer disease management.
authors: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
authors: Anas
authors: A
authors: Subarnas
authors: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
authors: Ajeng
authors: A
authors: Diantini
authors: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia; Department of Public Health, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan.
authors: Rizky
authors: R
authors: Abdulah
authors: Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
authors: Ade
authors: A
authors: Zuhrotun
authors: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
authors: Patria A
authors: PA
authors: Nugraha
authors: Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan.
authors: Yuni E
authors: YE
authors: Hadisaputri
authors: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia; Department of Public Health, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan.
authors: Irma M
authors: IM
authors: Puspitasari
authors: Department of Public Health, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan.
authors: Chiho
authors: C
authors: Yamazaki
authors: Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan.
authors: Hiroyuki
authors: H
authors: Kuwano
authors: Department of Public Health, Gunma University Graduate School of Medicine, Gunma 371-8511, Japan.
authors: Hiroshi
authors: H
authors: Koyama
keywords: Garcinia celebica; apoptosis; cancer; primates