title: A novel CHH gene from the Pacific white shrimp Litopenaeus vannamei was characterized and found highly expressed in gut and less in eyestalk and other extra-eyestalk tissues.
abstract: The crustacean hyperglycemic hormone (CHH) family is an important group of neuropeptides involved in controlling growth, reproduction, and stress response in decapod species. In this study, a new gene containing 4 exons-3 introns flanked by canonical 5'-GT-AG-3' intron splice-site junctions was isolated from Litopenaeus vannamei. Two full length transcripts of this CHH were isolated from eyestalk and pericardial tissue of males and females using rapid amplification of cDNA ends (RACE). Transcripts sequences were 1578bp in length in males pericardial tissues and in males and females eyestalk with 100% identity, but the transcript isolated from females pericardial tissues was shorter (974bp). The differences in transcripts length is a result of two polyadenylation sites present in the 3'UTR resulting in two transcription termination signals. Transcript sequences encoded one unique protein that can be classified as type I CHH subfamily because of the 4 exons and 3 introns structure, although the CPRP region is not-well conserved and there is no amidation in the C-terminal of the deduced amino acid sequence. Furthermore, there is a glycine inserted in the mature peptide not at position 12 as in type II CHHs but after amino acid 31 and the phylogenetic analysis did not group the peptide within type I, but closer to type II CHHs. We demonstrated by endpoint-PCR, qPCR, and in situ hybridization (ISH), that this gene is expressed in neuroendocrine organs known to express CHHs in penaeid shrimp, including X-organ and optic nerve in eyestalk, supraesophageal ganglion (SoG), but it is also expressed in other organs as gill, gut, pericardial cavity, as well as in terminal ampoule or spermatophore and vas deferens of males.
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, C.P. 23096, La Paz, Baja California Sur, Mexico. Electronic address: cventura@cibnor.mx.
authors: Claudia
authors: C
authors: Ventura-López
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, C.P. 23096, La Paz, Baja California Sur, Mexico. Electronic address: ggomez@cibnor.mx.
authors: Gracia
authors: G
authors: Gómez-Anduro
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, C.P. 23096, La Paz, Baja California Sur, Mexico. Electronic address: farcos04@cibnor.mx.
authors: Fabiola G
authors: FG
authors: Arcos
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, C.P. 23096, La Paz, Baja California Sur, Mexico. Electronic address: raul.llera@gmail.com.
authors: Raúl
authors: R
authors: Llera-Herrera
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, C.P. 23096, La Paz, Baja California Sur, Mexico. Electronic address: iracotta@cibnor.mx.
authors: Ilie S
authors: IS
authors: Racotta
authors: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, C.P. 23096, La Paz, Baja California Sur, Mexico. Electronic address: aibarra@cibnor.mx.
authors: Ana M
authors: AM
authors: Ibarra
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004064:Digestive System / Q000378:metabolism*; D005123:Eye / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007447:Invertebrate Hormones / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics*; D033561:Penaeidae / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C015947:hyperglycemic hormone, crustacean
keywords: Crustacean-hyperglycemic-hormone; Differential expression; Ductus-deferens; Extra-eyestalk-expression; Foregut; ISH; Pericardial-organ; Spermatophore