title: Highly selective capture of nucleosides with boronic acid functionalized polymer brushes prepared by atom transfer radical polymerization.
abstract: The nucleoside or modified nucleoside level in biological fluids reflects the pathological or physiological state of the body. Boronate affinity absorbents are widely used to selectively extract nucleosides from complex samples. In this work, a novel functionalized absorbent was synthesized by attaching 4-mercaptophenylboronic acid to gold nanoparticles on modified attapulgite. The surface of the attapulgite was modified by poly(acryloyloxyethyltrimethyl ammonium chloride) by atom transfer radical polymerization, creating many polymer brushes on the surface. The resultant material exhibited superior binding capacity (30.83 mg/g) for adenosine and was able to capture cis-diol nucleosides from 1000-fold interferences. Finally, to demonstrate its potential for biomolecule extraction, this boronate affinity material was used to preconcentrate nucleosides from human urine and plasma.
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China.
authors: Ting
authors: T
authors: Cheng
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China.
authors: Shuqiang
authors: S
authors: Zhu
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China.
authors: Bin
authors: B
authors: Zhu
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China.
authors: Xiaoyan
authors: X
authors: Liu
authors: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, China.
authors: Haixia
authors: H
authors: Zhang
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry*; D001897:Boronic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D064368:Healthy Volunteers; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D009705:Nucleosides / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification* / Q000652:urine; D058105:Polymerization; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: C510906:4-mercaptophenylboronic acid; D000180:Acrylic Resins; D001897:Boronic Acids; D009705:Nucleosides; D000644:Quaternary Ammonium Compounds; D013438:Sulfhydryl Compounds; D006046:Gold
keywords: Attapulgite; Boronic acid; Gold nanoparticles; Nucleosides