title: Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus.
abstract: Glyphosate (GLY) is the most used herbicide worldwide and its effects on anurans are well known. Pollutants can cause physiological and morphological effects. Therefore, this study evaluated the effects of GLY on hepatic melanomacrophages as a response to environmental stressors. Three treatments were exposed to different concentrations of pure GLY (100, 1000, and 10,000 μg g(-1), respectively), and there was also a control group. After the experimental time, liver and blood were analyzed. Melanomacrophages (MMCs) were located between the hepatocyte cordons, close to sinusoids. GLY increased the melanin area in MMCs of Leptodactylus latinasus exposed since lowest concentration until highest concentration. GLY also changed the occurrence of hepatic catabolism pigments into melanomacrophages and erythrocyte nuclear abnormalities; therefore, it can interfere with the hepatic metabolism. In conclusion, GLY promotes alterations in the hepatic tissue and erythrocyte nuclear abnormalities. Furthermore, MMCs may be useful as morphological responses of GLY effects.
authors: PROICO 9401, Área Zoología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, San Luis, 5700, Argentina.|Centro de Investigaciones del Medio Ambiente, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Argentina.
authors: Juan Manuel
authors: JM
authors: Pérez-Iglesias
authors: Department of Biology, São Paulo State University (UNESP), IBILCE, Rua Cristóvão Colombo, 2265, Jardim Nazareth, São José do Rio Preto, 15054-000, São Paulo, Brazil.
authors: Lilian
authors: L
authors: Franco-Belussi
authors: PROICO 9401, Área Zoología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, San Luis, 5700, Argentina.
authors: Liliana
authors: L
authors: Moreno
authors: PROICO 9401, Área Zoología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, San Luis, 5700, Argentina.
authors: Susana
authors: S
authors: Tripole
authors: Department of Biology, São Paulo State University (UNESP), IBILCE, Rua Cristóvão Colombo, 2265, Jardim Nazareth, São José do Rio Preto, 15054-000, São Paulo, Brazil. classius@ibilce.unesp.br.
authors: Classius
authors: C
authors: de Oliveira
authors: PROICO 9401, Área Zoología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, San Luis, 5700, Argentina.|Centro de Investigaciones del Medio Ambiente, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Argentina.
authors: Guillermo Sebastián
authors: GS
authors: Natale
mesh_terms: D000818:Animals; D001001:Anura; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008543:Melanins / Q000378:metabolism*; D014329:Tropical Climate; D000097797:Glyphosate
chemical_list: D004785:Environmental Pollutants; D005998:Glycine; D008543:Melanins; D000097797:Glyphosate
keywords: Genotoxic effects; Glyphosate; Hemosiderin; Melanin; Melanomacrophages; Morphological responses