title: Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation.
abstract: We previously established a spermatogonial transplantation model in fish using triploid recipients. Although triploid salmonids are sterile, they carry a limited number of immature triploid germ cells that potentially compete with the donor-derived germ cells for their niche. We therefore assessed the biological characteristics of germ cell-deficient gonads in rainbow trout for their suitability as recipients for germ cell transplantation in this study. Antisense morpholino oligonucleotides against the dead end gene were microinjected into the fertilized eggs of rainbow trout to eliminate endogenous germ cells, leaving only their supporting cells. Unlike similar approaches performed in zebrafish and medaka, these germ cell-deficient rainbow trout did not show a male-biased sex ratio. Approximately 30,000 spermatogonia were then transplanted into the body cavities of both germ cell-deficient and control recipients. The donor-derived germ cells showed significantly higher proliferation in the gonads of germ cell-deficient recipients than those in the gonads of the control recipients. Finally, the applicability of the germ cell-deficient recipients for xenogeneic transplantation was evaluated by transplanting rainbow trout spermatogonia into germ cell-deficient masu salmon recipients. The resulting recipient salmon matured normally and produced trout gametes, and early survival of the resulting trout offspring was as high as that of the control offspring. Thus, dead end-knockdown salmonids appear to be ideal recipients for the intraperitoneal transplantation of spermatogonia.
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors: Goro
authors: G
authors: Yoshizaki
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors: Kuniko
authors: K
authors: Takashiba
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors: Shotaro
authors: S
authors: Shimamori
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors: Kiyoko
authors: K
authors: Fujinuma
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors: Shinya
authors: S
authors: Shikina
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors: Tomoyuki
authors: T
authors: Okutsu
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors: Sachi
authors: S
authors: Kume
authors: Tokyo University of Marine Science and Technology, Tokyo, Japan.|Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, Ibaraki, Japan.
authors: Makoto
authors: M
authors: Hayashi
mesh_terms: D000818:Animals; D005260:Female; D055785:Gene Knockdown Techniques; D008297:Male; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000601:surgery; D013093:Spermatogonia / Q000166:cytology / Q000637:transplantation*; D014183:Transplantation, Heterologous / Q000662:veterinary