title: Genomic Organization of Human Transcription Initiation Complexes.
abstract: A repertoire of transcription initiation factors engage the core promoter of mRNA genes to recruit RNA polymerase (Pol) II to initiate transcription, yet their precise spatial organization remains unclear. Using ChIP-exo, here we detail the interactions and genomic organization of initiation factors TBP, TFIIB, and Pol II at mRNA genes and within CpG islands. We find that when Pol II moves into a transcriptionally paused state, TBP/TFIIB remain at the promoter. We show that TBP and TFIIB bound to the core promoter at two separate, resolvable locations that coincided with sites of divergent transcription initiation. We also examine the precise binding of TBP at Pol III transcribed tRNA genes. We find that TBP crosslinked to tRNA genes in a similar manner as at Pol II transcribed genes. This comprehensive and high resolution genome-wide detection of the initiation machinery produces a consolidated view of transcription initiation events humans at Pol II coding and Pol III transcribed tRNA genes.
authors: Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America.
authors: B Franklin
authors: BF
authors: Pugh
authors: Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America.
authors: Bryan J
authors: BJ
authors: Venters
mesh_terms: D000906:Antibodies / Q000737:chemistry; D045744:Cell Line, Tumor; D018899:CpG Islands; D004268:DNA-Binding Proteins / Q000378:metabolism; D015894:Genome, Human; D023281:Genomics; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020014:K562 Cells; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics; D016415:Sequence Alignment; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism; D035581:Transcription Factor TFIIB / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000906:Antibodies; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D035181:TATA-Box Binding Protein; C509817:TBP protein, human; D035581:Transcription Factor TFIIB; D012343:RNA, Transfer; D012319:RNA Polymerase II