title: Antiproliferative effects of triterpenoidal derivatives, obtained from the marine sponge Siphonochalina sp., on human hepatic and colorectal cancer cells.
abstract: Three triterpenoidal derivatives [Sipholenol A (1), sipholenol L (2) and sipholenone A (3)] were isolated from the Red Sea sponge Siphonochalina sp. The structures were determined based on spectroscopic measurements (NMR, UV, IR and MS). The isolated compounds were evaluated for their cytotoxic activity against three cancer cell lines; HepG2, Caco-2 and HT-29. Moreover, the effects of these metabolites on cell cycle progression as well as cell cycle regulating proteins were assessed. Compounds 1, 2 and 3 showed moderate activity against HepG2 cells with IC(50) values of 17.18 ± 1.18, 24.01 ± 0.59 and 35.06 ± 1.10 μM, respectively. Compounds 1 and 2 exerted a considerable antiproliferative effect with IC(50) values of 4.80 ± 0.18 and 26.64 ± 0.30 μM, respectively, against Caco-2 cells. Finally, 1 and 2 exhibited antiproliferative activity against colorectal cancer cells (HT-29) with IC(50) values of 24.65 ± 0.80 and 4.48 ± 0.1 μM, respectively. Cell cycle analysis indicated that these compounds induced cell cycle arrest particularly in G0/G1 and S phases. Furthermore, the triterpenoids increased the expression of cyclin-B1, cyclin-D1 and cleaved caspase-3, as determined by immunofluorescence, indicating an important role of apoptosis in cell death induced by these compounds.
authors: Ahmed
authors: A
authors: Abdel-Lateff
authors: Ahmed M
authors: AM
authors: Al-Abd
authors: Abdulrahman M
authors: AM
authors: Alahdal
authors: Walied M
authors: WM
authors: Alarif
authors: Seif-Eldin N
authors: SE
authors: Ayyad
authors: Sultan S
authors: SS
authors: Al-Lihaibi
authors: Mohamed E
authors: ME
authors: Hegazy
authors: Ameen
authors: A
authors: Al Mohammadi
authors: Tamer M
authors: TM
authors: Abdelghany
authors: Ashraf B
authors: AB
authors: Abdel-Naim
authors: Mohamed A A
authors: MA
authors: Moustafa
authors: Zainy M
authors: ZM
authors: Banjer
authors: Ahmad S
authors: AS
authors: Azhar
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D011161:Porifera / Q000737:chemistry; D013729:Terpenes / Q000008:administration & dosage*
chemical_list: D013729:Terpenes