title: Two nonspecific lipid transfer proteins (nsLTPs) from tomato seeds are associated to severe symptoms of tomato-allergic patients.
abstract: Tomato has become one of the most consumed vegetables worldwide, but its intake is often accompanied by an increasing risk of inducing allergic symptoms. The aim of the work was the identification of new seed-specific allergens associated with severe symptoms in patients allergic to this edible vegetable.
  
  
  
  We used 22 sera from well-defined tomato-allergic patients. Tomato seed proteins were purified and analyzed for biochemical and immunological characterization. A mixture of two associated IgE-binding nsLTPs was purified from tomato seeds. Both allergens, Sola l 7 and Sola l 6, displayed primary structure differences with respect to their counterpart, Sola l 3, from tomato pulp/peel. They retained the ability to bind IgE from 71.4% of patients with severe symptoms. The purified proteins induced positive basophil activation test and skin prick test, and displayed cross-reactivity with homologous allergens from peanut and sunflower seeds, among others.
  
  
  
  We herein described two novel allergens from tomato seeds that belong to the nonspecific lipid transfer protein family classes 1 and 2, respectively. This is the first work associating IgE reactivity to these proteins with severe symptoms of certain tomato-allergic patients. Therefore, they are optimal candidates for clarifying the diagnosis of the tomato allergy.
authors: Faculty of Chemistry, Department of Biochemistry and Molecular Biology I, Complutense University of Madrid, Madrid, Spain.
authors: Laura
authors: L
authors: Martín-Pedraza
authors: Allergy Research Laboratory, IBIMA, Regional University Hospital of Málaga, UMA, Málaga, Spain.
authors: Miguel
authors: M
authors: González
authors: U.G.C. Allergy, IBIMA, Regional University Hospital of Málaga, UMA, Málaga, Spain.
authors: Francisca
authors: F
authors: Gómez
authors: Allergy Unit, Infanta Leonor Hospital, Madrid, Spain.
authors: Natalia
authors: N
authors: Blanca-López
authors: Center for Plant Biotechnology and Genomic (UPM-INIA), Madrid, Spain.
authors: María
authors: M
authors: Garrido-Arandia
authors: Faculty of Chemistry, Department of Biochemistry and Molecular Biology I, Complutense University of Madrid, Madrid, Spain.
authors: Rosalía
authors: R
authors: Rodríguez
authors: U.G.C. Allergy, IBIMA, Regional University Hospital of Málaga, UMA, Málaga, Spain.
authors: María J
authors: MJ
authors: Torres
authors: U.G.C. Allergy, IBIMA, Regional University Hospital of Málaga, UMA, Málaga, Spain.
authors: Miguel
authors: M
authors: Blanca
authors: Faculty of Chemistry, Department of Biochemistry and Molecular Biology I, Complutense University of Madrid, Madrid, Spain.
authors: Mayte
authors: M
authors: Villalba
authors: Allergy Research Laboratory, IBIMA, Regional University Hospital of Málaga, UMA, Málaga, Spain.|U.G.C. Allergy, IBIMA, Regional University Hospital of Málaga, UMA, Málaga, Spain.
authors: Cristobalina
authors: C
authors: Mayorga
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D018551:Solanum lycopersicum / Q000737:chemistry / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology; D012639:Seeds / Q000737:chemistry / Q000276:immunology*; D017422:Sequence Analysis, DNA; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000485:Allergens; D002352:Carrier Proteins; D010940:Plant Proteins; C072087:sterol carrier proteins; D007073:Immunoglobulin E
keywords: Cross-reactivity; Tomato allergy; Tomato seeds; nsLTP class 1 Sola l 7; nsLTP class 2 Sola l 6