title: Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors.
abstract: Anterior definitive endoderm (ADE), the ventral foregut precursor, is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other organs. Here, a method is described for the differentiation of mouse embryonic stem cells (mESCs) to definitive endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (embryoid body) culture or in a serum-free adherent monolayer culture. ESC-derived ADE cells are committed to endodermal fates and can undergo further differentiation in vitro towards ventral foregut derivatives.
authors: Centre (DanStem), University of Copenhagen, Copenhagen, Denmark.
authors: Michaela
authors: M
authors: Rothová
authors: Centre (DanStem), University of Copenhagen, Copenhagen, Denmark.
authors: Jurriaan J
authors: JJ
authors: Hölzenspies
authors: MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom.
authors: Alessandra
authors: A
authors: Livigni
authors: MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom.|Instituto de Neurociencias, Consejo Superior de Investigaciones Cientificas, Universidad Miguel Hernandez de Elche, Alicante, Spain.
authors: Santiago Nahuel
authors: SN
authors: Villegas
authors: Centre (DanStem), University of Copenhagen, Copenhagen, Denmark.|MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom.
authors: Joshua M
authors: JM
authors: Brickman
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology; D004707:Endoderm / Q000166:cytology / Q000196:embryology; D006801:Humans; D007422:Intestines / Q000196:embryology; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism
keywords: FGF; Hhex; activin; anterior; differentiation; embryonic stem cells; endoderm