title: Genome-guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15-2(T) from Lop Nor region, China.
abstract: Microbial secondary metabolites are valuable resources for novel drug discovery. In particular, actinomycetes expressed a range of antibiotics against a spectrum of bacteria. In genus level, strain Allosalinactinospora lopnorensis CA15-2(T) is the first new actinomycete isolated from the Lop Nor region, China. Antimicrobial assays revealed that the strain could inhibit the growth of certain types of bacteria, including Acinetobacter baumannii and Staphylococcus aureus, highlighting its clinical significance. Here we report the 5,894,259 base pairs genome of the strain, containing 5,662 predicted genes, and 832 of them cannot be detected by sequence similarity-based methods, suggesting the new species may carry a novel gene pool. Furthermore, our genome-mining investigation reveals that A. lopnorensis CA15-2(T) contains 17 gene clusters coding for known or novel secondary metabolites. Meanwhile, at least six secondary metabolites were disclosed from ethyl acetate (EA) extract of the fermentation broth of the strain by high-resolution UPLC-MS. Compared with reported clusters of other species, many new genes were found in clusters, and the physical chromosomal location and order of genes in the clusters are distinct. This study presents evidence in support of A. lopnorensis CA15-2(T) as a potent natural products source for drug discovery.
authors: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors: Chen
authors: C
authors: Huang
authors: Stanley HoCentre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.|School of Public Health, The University of Hong Kong, Hong Kong.
authors: Ross Ka-Kit
authors: RK
authors: Leung
authors: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors: Min
authors: M
authors: Guo
authors: Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors: Li
authors: L
authors: Tuo
authors: Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors: Lin
authors: L
authors: Guo
authors: Stanley HoCentre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.
authors: Wing Wai
authors: WW
authors: Yew
authors: Faculty of Science and Technology, Department of Civil and Environmental Engineering, University of Macau, Macao, China.
authors: Inchio
authors: I
authors: Lou
authors: State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors: Simon Ming Yuen
authors: SMY
authors: Lee
authors: Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors: Chenghang
authors: C
authors: Sun
mesh_terms: D000085:Acetates; D040981:Acinetobacter baumannii / Q000254:growth & development; D039903:Actinobacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D000898:Antibiosis; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification*; D002681:China; D002874:Chromosome Mapping; D052978:Disk Diffusion Antimicrobial Tests; D055808:Drug Discovery; D059646:Genome Size; D016680:Genome, Bacterial; D005810:Multigene Family; D010802:Phylogeny; D064210:Secondary Metabolism; D017422:Sequence Analysis, DNA; D012997:Solvents; D013211:Staphylococcus aureus / Q000254:growth & development
chemical_list: D000085:Acetates; D000900:Anti-Bacterial Agents; D001688:Biological Products; D012997:Solvents; C007650:ethyl acetate