title: Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease.
abstract: OBJECTIVE
  To better characterize posterior uveitis, vitreous samples from 15 patients were subjected to antibody arrays, and the expression levels of 200 human cytokines were evaluated. Expression was analyzed by 1-way analysis of variance (significance at P < .01), unsupervised cluster algorithm, and pathway analysis.
  
  
  METHODS
  Unbiased clustering of patients, based on their cytokine expression profile, suggested that particular protein networks and molecular pathways are altered in various forms of uveitis. Expression of interleukin 23 (IL-23), IL-1 receptor I (IL-1RI), IL-17R, tissue inhibitors of metalloproteinase 1 and 2 (TIMP-1 and TIMP-2), insulinlike growth factor-binding protein 2 (IGFBP-2), nerve growth factor (b-NGF), platelet-derived growth factor receptor β polypeptide (PDGFRb), bone morphogenic protein 4 (BMP-4), and stem cell factor (SCF) constituted a common cytokine signature in the vitreous of patients with uveitis. In 1 patient with progressive, idiopathic visual loss, this last-line analysis implicated retinal autoimmunity, a diagnosis that was validated when her serum sample was found to contain antibodies to S-arrestin, a retinal protein and potent cause of autoimmune retinal degeneration.
  
  
  CONCLUSIONS
  The analysis identifies a common cytokine signature for posterior uveitis and guides the diagnosis of a patient with idiopathic uveitis. Personalized treatment reversed the visual loss, illustrating how proteomic tools may individualize therapy.
authors: Omics Laboratory, University of Iowa Carver College of Medicine, Iowa City2Department of Ophthalmology and Visual Sciences, University of Iowa Carver College of Medicine, Iowa City3Medical Scientist Training Program, University of Iowa Carver College of M.
authors: Gabriel
authors: G
authors: Velez
authors: Omics Laboratory, University of Iowa Carver College of Medicine, Iowa City2Department of Ophthalmology and Visual Sciences, University of Iowa Carver College of Medicine, Iowa City.
authors: C Nathaniel
authors: CN
authors: Roybal
authors: Omics Laboratory, University of Iowa Carver College of Medicine, Iowa City2Department of Ophthalmology and Visual Sciences, University of Iowa Carver College of Medicine, Iowa City.
authors: Diana
authors: D
authors: Colgan
authors: Barbara and Donald Jonas Laboratory of Stem Cells and Regenerative Medicine and Bernard and Shirlee Brown Glaucoma Laboratory, Edward S. Harkness Eye Institute, Columbia University, New York, New York5Department of Pathology and Cell Biology, College of P.
authors: Stephen H
authors: SH
authors: Tsang
authors: Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City.
authors: Alexander G
authors: AG
authors: Bassuk
authors: Omics Laboratory, University of Iowa Carver College of Medicine, Iowa City2Department of Ophthalmology and Visual Sciences, University of Iowa Carver College of Medicine, Iowa City.
authors: Vinit B
authors: VB
authors: Mahajan
mesh_terms: D000305:Adrenal Cortex Hormones / Q000008:administration & dosage*; D000328:Adult; D000704:Analysis of Variance; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism*; D005500:Follow-Up Studies; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D008297:Male; D008875:Middle Aged; D057285:Precision Medicine / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012016:Reference Values; D018570:Risk Assessment; D012720:Severity of Illness Index; D016896:Treatment Outcome; D014605:Uveitis / Q000175:diagnosis / Q000188:drug therapy*; D014792:Visual Acuity / Q000187:drug effects
chemical_list: D000305:Adrenal Cortex Hormones; D016207:Cytokines; D007166:Immunosuppressive Agents