title: Comparative study of CXC chemokines modulation in brown trout (Salmo trutta) following infection with a bacterial or viral pathogen.
abstract: Chemokine modulation in response to pathogens still needs to be fully characterised in fish, in view of the recently described novel chemokines present. This paper reports the first comparative study of CXC chemokine genes transcription in salmonids (brown trout), with a particular focus on the fish specific CXC chemokines (CXCL_F). Adopting new primer sets, optimised to specifically target mRNA, a RT-qPCR gene screening was carried out. Constitutive gene expression was assessed first in six tissues from SPF brown trout. Transcription modulation was next investigated in kidney and spleen during septicaemic infection induced by a RNA virus (Viral Haemorrhagic Septicaemia virus, genotype Ia) or by a Gram negative bacterium (Yersinia ruckeri, ser. O1/biot. 2). From each target organ specific pathogen burden, measured detecting VHSV-glycoprotein or Y. ruckeri 16S rRNA, and IFN-γ gene expression were analysed for their correlation to chemokine transcription. Both pathogens modulated CXC chemokine gene transcript levels, with marked up-regulation seen in some cases, and with both temporal and tissue specific effects apparent. For example, Y. ruckeri strongly induced chemokine transcription in spleen within 24h, whilst VHS generally induced the largest increases at 3d.p.i. in both tissues. This study gives clues to the role of the novel CXC chemokines, in comparison to the other known CXC chemokines in salmonids.
authors: Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Scotland, UK; CEFAS Weymouth Laboratory, The Nothe, Weymouth, Dorset, England, UK.
authors: Bartolomeo
authors: B
authors: Gorgoglione
authors: Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Egypt.
authors: Eman
authors: E
authors: Zahran
authors: CEFAS Weymouth Laboratory, The Nothe, Weymouth, Dorset, England, UK.
authors: Nick G H
authors: NGH
authors: Taylor
authors: CEFAS Weymouth Laboratory, The Nothe, Weymouth, Dorset, England, UK.
authors: Stephen W
authors: SW
authors: Feist
authors: Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Scotland, UK. Electronic address: j.j.zou@abdn.ac.uk.
authors: Jun
authors: J
authors: Zou
authors: Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen, Scotland, UK. Electronic address: c.secombes@abdn.ac.uk.
authors: Christopher J
authors: CJ
authors: Secombes
mesh_terms: D000818:Animals; D019743:Chemokines, CXC / Q000235:genetics*; D005260:Female; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology*; D029141:Novirhabdovirus / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome; D014337:Trout; D015009:Yersinia Infections / Q000235:genetics / Q000276:immunology*; D044124:Yersinia ruckeri
chemical_list: D019743:Chemokines, CXC
keywords: Brown trout; Chemokines; Enteric Red Mouth disease; Gene expression; Innate immunity; Viral Haemorrhagic Septicaemia