title: Cell-substrate impedance fluctuations of single amoeboid cells encode cell-shape and adhesion dynamics.
abstract: We show systematic electrical impedance measurements of single motile cells on microelectrodes. Wild-type cells and mutant strains were studied that differ in their cell-substrate adhesion strength. We recorded the projected cell area by time-lapse microscopy and observed irregular oscillations of the cell shape. These oscillations were correlated with long-term variations in the impedance signal. Superposed to these long-term trends, we observed fluctuations in the impedance signal. Their magnitude clearly correlated with the adhesion strength, suggesting that strongly adherent cells display more dynamic cell-substrate interactions.
authors: Institute of Physics and Astronomy, University of Potsdam, Karl-Liebknecht Strasse 24/25, 14476 Potsdam, Germany.
authors: Helmar
authors: H
authors: Leonhardt
authors: Institute of Physics and Astronomy, University of Potsdam, Karl-Liebknecht Strasse 24/25, 14476 Potsdam, Germany.
authors: Matthias
authors: M
authors: Gerhardt
authors: Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany.
authors: Nadine
authors: N
authors: Höppner
authors: Institute of Physics and Astronomy, University of Potsdam, Karl-Liebknecht Strasse 24/25, 14476 Potsdam, Germany.
authors: Kirsten
authors: K
authors: Krüger
authors: Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany.
authors: Marco
authors: M
authors: Tarantola
authors: Institute of Physics and Astronomy, University of Potsdam, Karl-Liebknecht Strasse 24/25, 14476 Potsdam, Germany.|Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077 Göttingen, Germany.
authors: Carsten
authors: C
authors: Beta
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D004023:Dictyostelium / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D017097:Electric Impedance; D008839:Microelectrodes; D008853:Microscopy; D018625:Microscopy, Atomic Force; D009154:Mutation; D010507:Periodicity; D013057:Spectrum Analysis; D013995:Time