title: Pulsed (13)C2-Acetate Protein-SIP Unveils Epsilonproteobacteria as Dominant Acetate Utilizers in a Sulfate-Reducing Microbial Community Mineralizing Benzene.
abstract: In a benzene-degrading and sulfate-reducing syntrophic consortium, a clostridium affiliated to the genus Pelotomaculum was previously described to ferment benzene while various sulfate-reducing Deltaproteobacteria and a member of the Epsilonproteobacteria were supposed to utilize acetate and hydrogen as key metabolites derived from benzene fermentation. However, the acetate utilization network within this community was not yet unveiled. In this study, we performed a pulsed (13)C2-acetate protein stable isotope probing (protein-SIP) approach continuously spiking low amounts of acetate (10 μM per day) in addition to the ongoing mineralization of unlabeled benzene. Metaproteomics revealed high abundances of Clostridiales followed by Syntrophobacterales, Desulfobacterales, Desulfuromonadales, Desulfovibrionales, Archaeoglobales, and Campylobacterales. Pulsed acetate protein-SIP results indicated that members of the Campylobacterales, the Syntrophobacterales, the Archaeoglobales, the Clostridiales, and the Desulfobacterales were linked to acetate utilization in descending abundance. The Campylobacterales revealed the fastest and highest (13)C incorporation. Previous experiments suggested that the activity of the Campylobacterales was not essential for anaerobic benzene degradation in the investigated community. However, these organisms were consistently detected in various hydrocarbon-degrading and sulfate-reducing consortia enriched from the same aquifer. Here, we demonstrate that this member of the Campylobacterales is the dominant acetate utilizer in the benzene-degrading microbial consortium.
authors: Institute for Animal Science, University of Hohenheim, Emil-Wolff-Str. 6-10, 70599, Stuttgart, Germany.|Department of Proteomics, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.
authors: Robert
authors: R
authors: Starke
authors: Department of Isotope Biogeochemistry, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.|Department of Environmental Microbiology, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.
authors: Andreas
authors: A
authors: Keller
authors: Department of Proteomics, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.
authors: Nico
authors: N
authors: Jehmlich
authors: Department of Isotope Biogeochemistry, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.
authors: Carsten
authors: C
authors: Vogt
authors: Department of Isotope Biogeochemistry, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.
authors: Hans H
authors: HH
authors: Richnow
authors: Department of Environmental Microbiology, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.
authors: Sabine
authors: S
authors: Kleinsteuber
authors: Department of Proteomics, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.|Department of Metabolomics, Helmholtz-Centre for Environmental Research (UFZ), Permoserstr. 15, 04318, Leipzig, Germany.
authors: Martin
authors: M
authors: von Bergen
authors: Institute for Animal Science, University of Hohenheim, Emil-Wolff-Str. 6-10, 70599, Stuttgart, Germany. jseifert@uni-hohenheim.de.
authors: Jana
authors: http://orcid.org/0000-0002-7690-8539
authors: J
authors: Seifert
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000032:analysis; D001554:Benzene / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002247:Carbon Isotopes / Q000032:analysis; D000068016:Eubacteriales / Q000254:growth & development / Q000378:metabolism; D020564:Deltaproteobacteria / Q000378:metabolism; D020565:Epsilonproteobacteria / Q000378:metabolism*; D060587:Groundwater / Q000382:microbiology; D006838:Hydrocarbons / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D059013:Microbial Consortia; D010802:Phylogeny; D040901:Proteomics / Q000379:methods*; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000378:metabolism
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D006838:Hydrocarbons; D013431:Sulfates; D006859:Hydrogen; D001554:Benzene
keywords: Anaerobic benzene degradation; Carbon flow; Metaproteomics