title: Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies.
abstract: Rust fungal pathogens of wheat (Triticum spp.) affect crop yields worldwide. The molecular mechanisms underlying the virulence of these pathogens remain elusive, due to the limited availability of suitable molecular genetic research tools. Notably, the inability to perform high-throughput analyses of candidate virulence proteins (also known as effectors) impairs progress. We previously established a pipeline for the fast-forward screens of rust fungal candidate effectors in the model plant Nicotiana benthamiana. This pipeline involves selecting candidate effectors in silico and performing cell biology and protein-protein interaction assays in planta to gain insight into the putative functions of candidate effectors. In this study, we used this pipeline to identify and characterize sixteen candidate effectors from the wheat yellow rust fungal pathogen Puccinia striiformis f sp tritici. Nine candidate effectors targeted a specific plant subcellular compartment or protein complex, providing valuable information on their putative functions in plant cells. One candidate effector, PST02549, accumulated in processing bodies (P-bodies), protein complexes involved in mRNA decapping, degradation, and storage. PST02549 also associates with the P-body-resident ENHANCER OF mRNA DECAPPING PROTEIN 4 (EDC4) from N. benthamiana and wheat. We propose that P-bodies are a novel plant cell compartment targeted by pathogen effectors.
authors: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.|INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.|Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.
authors: Benjamin
authors: B
authors: Petre
authors: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.|The Genome Analysis Centre, Norwich Research Park, Norwich, United Kingdom.|The John Innes Centre, Norwich Research Park, Norwich, United Kingdom.
authors: Diane G O
authors: DG
authors: Saunders
authors: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors: Jan
authors: J
authors: Sklenar
authors: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.|INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.|Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.
authors: Cécile
authors: C
authors: Lorrain
authors: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.|The Genome Analysis Centre, Norwich Research Park, Norwich, United Kingdom.
authors: Ksenia V
authors: KV
authors: Krasileva
authors: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors: Joe
authors: J
authors: Win
authors: INRA, UMR 1136 Interactions Arbres/Microorganismes, Centre INRA Nancy Lorraine, Champenoux, France.|Université de Lorraine, UMR 1136 Interactions Arbres/Microorganismes, Faculté des Sciences et Technologies, Vandoeuvre-lès-Nancy, France.
authors: Sébastien
authors: S
authors: Duplessis
authors: The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
authors: Sophien
authors: S
authors: Kamoun
mesh_terms: D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D002853:Chromatography, Liquid; D003198:Computer Simulation; D005656:Fungal Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D016366:Open Reading Frames; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D025941:Protein Interaction Mapping; D012333:RNA, Messenger / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000382:microbiology*; D014908:Triticum / Q000382:microbiology; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins