title: Neurogenic Effects of Cell-Free Extracts of Adipose Stem Cells.
abstract: Stem-cell-based therapies are regarded as promising treatments for neurological disorders, and adipose-derived stem cells (ASCs) are a feasible source of clinical application of stem cell. Recent studies have shown that stem cells have a therapeutic potential for use in the treatment of various illnesses through paracrine action. To examine the effects of cell components of ASCs on neural stem cells (NSCs), we treated cell-free extracts of ASCs (CFE-ASCs) containing various components with brain-derived NSCs. To elucidate the effects of CFE-ASCs in NSC proliferation, we treated mouse subventricular zone-derived cultured NSCs with various doses of CFE-ASCs. As a result, CFE-ASCs were found to induce the proliferation of NSCs under conditions of growth factor deprivation in a dose-dependent manner (p<0.01). CFE-ASCs increase the expression of neuron and astrocyte differentiation markers including Tuj-1 (p<0.05) and glial fibrillary acidic protein (p<0.01) without altering the cell's fate in differentiating NSCs. In addition, treatment with CFE-ASCs induces an increase in neurite numbers (p<0.01) and lengths of NSCs (p<0.05). Furthermore, CFE-ASCs rescue the hydrogen peroxide-induced reduction of NSCs' viability (p<0.05) and neurite branching (p<0.01). Findings from our study indicate that CFE-ASCs support the survival, proliferation and differentiation of NSCs accompanied with neurite outgrowth, suggesting that CFE-ASCs can modulate neurogenesis in the central nervous system.
authors: Department of Neurology, Seoul National University Hospital, Seoul, South Korea.
authors: Jae-Jun
authors: JJ
authors: Ban
authors: Department of Medicine, University of Chicago, Chicago, IL, United States of America.
authors: Seungwon
authors: S
authors: Yang
authors: Department of Neurology, Seoul National University Hospital, Seoul, South Korea.
authors: Wooseok
authors: W
authors: Im
authors: Department of Neurology, Seoul National University Hospital, Seoul, South Korea.|Protein Metabolism Medical Research Center, College of Medicine, Seoul National University, Seoul, South Korea.
authors: Manho
authors: M
authors: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002474:Cell-Free System; D003470:Culture Media; D006801:Humans; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology
chemical_list: D003470:Culture Media