title: [Experimental Evaluation of Radioprotective Efficacy of Synthetic Genistein on Criteria of Glutathione System and Lipid Peroxidation in Erythrocytes of Peripheral Blood in Irradiated Rats].
abstract: The study was aimed to evaluate experimentally the radioprotective effectiveness of synthetic genistein in terms of the glutathione system and lipid peroxidation in erythrocytes of irradiated rats. The animals were exposed to single acute X-ray irradiation at a dose of 6 Gy. Genistein was administered intraperitoneally at 200 mg/kg 1 hour before radiation exposure. The irradiation caused the initiation of lipid peroxidation in the background depletion of reduced glutathione. Decrease by 25% in the number of malondialdehyde in the rats treated with genistein was registered 5 min after irradiation compared with the control. It is established thatl day after irradiation the level of reduced glutathione in the rats treated with genistein was 26% higher. However, intraperitoneal administration of genistein did not cause statistically significant changes in the activity of glutathione reductase, glutathione-S-transferase, or glucose-6-phosphate dehydrogenase during the whole period of observation. The results suggest that the radioprotective effect of synthetic genistein is implemented, along with other mechanisms, by stimulating the glutathione system and reducing the severity of lipid peroxidation.
authors: A N
authors: AN
authors: Grebenyuk
authors: R A
authors: RA
authors: Tarumov
authors: V A
authors: VA
authors: Basharin
authors: V U
authors: VU
authors: Kovtun
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D005720:Gamma Rays; D019833:Genistein / Q000008:administration & dosage* / Q000138:chemical synthesis; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000138:chemical synthesis; D051381:Rats; D014916:Whole-Body Irradiation
chemical_list: D011837:Radiation-Protective Agents; D019833:Genistein; D005982:Glutathione Transferase; D005978:Glutathione