title: Henneguya paraensis n. sp. (Myxozoa; Myxosporea), a new gill parasite of the Amazonian fish Cichla temensis (Teleostei: Cichlidae): morphological and molecular aspects.
abstract: The present study describes light microscopy, transmission electron microscopy, and molecular analyses of a myxosporid found parasitizing the gill region of the teleost fish Cichla temensis, collected from the Tocantins River, near Cametá, Pará State, Brazil. The prevalence of infection was 60 %. The spore-containing cysts that were located in the gill lamellae were oval and whitish. The spores had a mean length of 42.3 ± 0.65 μm; fusiform body, 12.8 ± 0.42-μm long and 8.6 ± 0.32-μm wide; each of the two valves exhibited a tapering tail of 29.5 ± 0.73 μm length. The spores had two polar capsules, 7.4 ± 0.16-μm long by 2.6 ± 0.08-μm wide, containing a polar filament with 5-7 twists. The spores differ from the species previously described, and phylogenetic analysis based on spore morphology and molecular aspects indicated that the fish parasite Henneguya sp. has a strong trend to form clades mainly based on the environment and host order/family. Thus, we conclude that the species belongs to the family Myxobolidae, genus Henneguya, which comprises a new species: Henneguya paraensis n. sp.
authors: Carlos Azevedo Research Laboratory, Federal Rural University of the Amazon (UFRA), Avenida Presidente Tancredo Neves, 2501, Montese, Belém, 66077-901, Pará, Brazil.
authors: Michele
authors: M
authors: Velasco
authors: Laboratory of Morphophysiology and Animal Health, Amapá State University (UEAP), Macapá, Amapá, Brazil.
authors: Marcela
authors: M
authors: Videira
authors: Laboratory of Biomolecular Technology, Federal University of Pará (UFPA), Belém, Pará, Brazil.
authors: Luciana de Cássia Silva do
authors: Lde C
authors: Nascimento
authors: Edilson Matos Research Laboratory (UFPA), Belém, Pará, Brazil.
authors: Patrícia
authors: P
authors: Matos
authors: Laboratory of Biomolecular Technology, Federal University of Pará (UFPA), Belém, Pará, Brazil.
authors: Evonnildo Costa
authors: EC
authors: Gonçalves
authors: Carlos Azevedo Research Laboratory, Federal Rural University of the Amazon (UFRA), Avenida Presidente Tancredo Neves, 2501, Montese, Belém, 66077-901, Pará, Brazil. edilson.matos9@gmail.com.
authors: Edilson
authors: E
authors: Matos
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D023681:Cichlids / Q000469:parasitology*; D005393:Fish Diseases / Q000453:epidemiology* / Q000469:parasitology*; D005880:Gills / Q000469:parasitology*; D046529:Microscopy, Electron, Transmission; D055764:Myxozoa / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D045483:Rivers / Q000469:parasitology; D013170:Spores / Q000166:cytology
keywords: Amazon; Gills; Myxozoa; New species; Parasite; Ultrastructure