title: In vivosubcellular resolution optical imaging in the lung reveals early metastatic proliferation and motility.
abstract: To better understand breast cancer metastatic cell seeding, we have employed multiphoton microscopy and a vacuum stabilized window which eliminates the need for complex registration software, video rate microscopy or specialized gating electronics to observe the initial steps of tumor cell seeding within the living, breathing lung. We observe that upon arrival to the lung, tumor cells are found exclusively in capillary vessels, completely fill their volume and display an initial high level of protrusive activity that dramatically reduces over time. Further, we observe a concomitant increase in positional stability during this same period. We employ several techniques accessible to most imaging labs for optimizing signal to noise and resolution which enable us to report the first direct observation, with subcellular resolution, of the arrival, proliferation, and motility of metastatic tumor cells within the lung.
authors: Department of Anatomy and Structural Biology; Albert Einstein College of Medicine; Bronx, NY USA; Gruss Lipper Biophotonics Center; Albert Einstein College of Medicine; Bronx, NY USA; Integrated Imaging Program; Albert Einstein College of Medicine; Bronx, NY USA.
authors: David
authors: D
authors: Entenberg
authors: Department of Developmental and Molecular Biology and the Department of Obstetrics/Gynecology and Woman's Health; Albert Einstein College of Medicine; Bronx, NY, USA.
authors: Carolina
authors: C
authors: Rodriguez-Tirado
authors: Department of Developmental and Molecular Biology and the Department of Obstetrics/Gynecology and Woman's Health; Albert Einstein College of Medicine; Bronx, NY, USA.
authors: Yu
authors: Y
authors: Kato
authors: Department of Developmental and Molecular Biology and the Department of Obstetrics/Gynecology and Woman's Health; Albert Einstein College of Medicine; Bronx, NY, USA; Medical Research Council Centre for Reproductive Health; Queen's Medical Research Institute; University of Edinburgh; Edinburgh, UK.
authors: Takanori
authors: T
authors: Kitamura
authors: Department of Developmental and Molecular Biology and the Department of Obstetrics/Gynecology and Woman's Health; Albert Einstein College of Medicine; Bronx, NY, USA; Medical Research Council Centre for Reproductive Health; Queen's Medical Research Institute; University of Edinburgh; Edinburgh, UK.
authors: Jeffrey W
authors: JW
authors: Pollard
authors: Department of Anatomy and Structural Biology; Albert Einstein College of Medicine; Bronx, NY USA; Gruss Lipper Biophotonics Center; Albert Einstein College of Medicine; Bronx, NY USA; Integrated Imaging Program; Albert Einstein College of Medicine; Bronx, NY USA.
authors: John
authors: J
authors: Condeelis
keywords: Intravital imaging; cancer; macrophage; metastasis; multiphoton; vacuum window