title: Microalgal bioengineering for sustainable energy development: Recent transgenesis and metabolic engineering strategies.
abstract: Exploring the efficiency of algae to produce remarkable products can be directly benefitted by studying its mechanism at systems level. Recent advents in biotechnology like flux balance analysis (FBA), genomics and in silico proteomics minimize the wet lab exertion. It is understood that FBA predicts the metabolic products, metabolic pathways and alternative pathway to maximize the desired product, and these are key components for microalgae bio-engineering. This review encompasses recent transgenesis techniques and metabolic engineering strategies applied to different microalgae for improving different traits. Further it also throws light on RNAi and riboswitch engineering based methods which may be advantageous for high throughput microalgal research. A valid and optimally designed microalga can be developed where every engineering strategies meet each other successfully and will definitely fulfill the market needs. It is also to be noted that Omics (viz. genetic and metabolic manipulation with bioinformatics) should be integrated to develop a strain which could prove to be a futuristic solution for sustainable development for energy.
authors: Department of Environmental Science & Engineering, Indian School of Mines, Dhanbad, Jharkhand, India.
authors: Chiranjib
authors: C
authors: Banerjee
authors: Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, India.
authors: Puneet Kumar
authors: PK
authors: Singh
authors: Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, India. pratyoosh.shukla@gmail.com.
authors: Pratyoosh
authors: P
authors: Shukla
mesh_terms: D057005:Bioengineering / Q000379:methods; D003198:Computer Simulation; D003247:Conservation of Natural Resources / Q000379:methods*; D018014:Gene Transfer Techniques; D023281:Genomics; D060847:Metabolic Engineering / Q000379:methods*; D058086:Microalgae / Q000235:genetics / Q000254:growth & development*; D040901:Proteomics; D058928:Riboswitch / Q000235:genetics
chemical_list: D058928:Riboswitch
keywords: Flux balance analysis (FBA); Riboswitch engineering; Synthetic biology; Transgenic microalgae