title: Intestinal Microbiota Distinguish Gout Patients from Healthy Humans.
abstract: Current blood-based approach for gout diagnosis can be of low sensitivity and hysteretic. Here via a 68-member cohort of 33 healthy and 35 diseased individuals, we reported that the intestinal microbiota of gout patients are highly distinct from healthy individuals in both organismal and functional structures. In gout, Bacteroides caccae and Bacteroides xylanisolvens are enriched yet Faecalibacterium prausnitzii and Bifidobacterium pseudocatenulatum depleted. The established reference microbial gene catalogue for gout revealed disorder in purine degradation and butyric acid biosynthesis in gout patients. In an additional 15-member validation-group, a diagnosis model via 17 gout-associated bacteria reached 88.9% accuracy, higher than the blood-uric-acid based approach. Intestinal microbiota of gout are more similar to those of type-2 diabetes than to liver cirrhosis, whereas depletion of Faecalibacterium prausnitzii and reduced butyrate biosynthesis are shared in each of the metabolic syndromes. Thus the Microbial Index of Gout was proposed as a novel, sensitive and non-invasive strategy for diagnosing gout via fecal microbiota.
authors: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Huhhot, Inner Mongolia, 010018, China.
authors: Zhuang
authors: Z
authors: Guo
authors: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Huhhot, Inner Mongolia, 010018, China.
authors: Jiachao
authors: J
authors: Zhang
authors: The First Affiliated Hospital, Baotou Medical College, Baotou, Inner Mongolia, 014010, China.
authors: Zhanli
authors: Z
authors: Wang
authors: Department of Microbiology, Yong Loo Li School of Medicine, National University of Singapore, 5 Science Drive 2, 117597, Singapore.
authors: Kay Ying
authors: KY
authors: Ang
authors: Single-Cell Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
authors: Shi
authors: S
authors: Huang
authors: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Huhhot, Inner Mongolia, 010018, China.
authors: Qiangchuan
authors: Q
authors: Hou
authors: Single-Cell Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
authors: Xiaoquan
authors: X
authors: Su
authors: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Huhhot, Inner Mongolia, 010018, China.
authors: Jianmin
authors: J
authors: Qiao
authors: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Huhhot, Inner Mongolia, 010018, China.
authors: Yi
authors: Y
authors: Zheng
authors: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Huhhot, Inner Mongolia, 010018, China.
authors: Lifeng
authors: L
authors: Wang
authors: Department of Microbiology, Yong Loo Li School of Medicine, National University of Singapore, 5 Science Drive 2, 117597, Singapore.
authors: Eileen
authors: E
authors: Koh
authors: Department of Microbiology, Yong Loo Li School of Medicine, National University of Singapore, 5 Science Drive 2, 117597, Singapore.
authors: Ho
authors: H
authors: Danliang
authors: Single-Cell Center, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, 266101, China.
authors: Jian
authors: J
authors: Xu
authors: Department of Microbiology, Yong Loo Li School of Medicine, National University of Singapore, 5 Science Drive 2, 117597, Singapore.
authors: Yuan Kun
authors: YK
authors: Lee
authors: Key Laboratory of Dairy Biotechnology and Bioengineering, Education Ministry of P. R. China, Huhhot, Inner Mongolia, 010018, China.
authors: Heping
authors: H
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001439:Bacteroides / Q000235:genetics / Q000302:isolation & purification; D000070257:Bifidobacterium pseudocatenulatum / Q000235:genetics / Q000302:isolation & purification; D015415:Biomarkers / Q000032:analysis; D002087:Butyrates / Q000378:metabolism; D016022:Case-Control Studies; D015331:Cohort Studies; D000070037:Faecalibacterium prausnitzii / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome; D006073:Gout / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012372:ROC Curve; D017422:Sequence Analysis, DNA; D014527:Uric Acid / Q000097:blood
chemical_list: D015415:Biomarkers; D002087:Butyrates; D012336:RNA, Ribosomal, 16S; D014527:Uric Acid