title: Pharmaceutical formulation of HSA hybrid coated iron oxide nanoparticles for magnetic drug targeting.
abstract: In this work we present a new formulation of superparamagnetic iron oxide nanoparticles (SPIONs) for magnetic drug targeting. The particles were reproducibly synthesized from current good manufacturing practice (cGMP) - grade substances. They were surface coated using fatty acids as anchoring molecules for human serum albumin. We comprehensively characterized the physicochemical core-shell structure of the particles using sophisticated methods. We investigated biocompatibility and cellular uptake of the particles using an established flow cytometric method in combination with microwave-plasma assisted atomic emission spectroscopy (MP-AES). The cytotoxic drug mitoxantrone was adsorbed on the protein shell and we showed that even in complex media it is slowly released with a close to zero order kinetics. We also describe an in vitro proof-of-concept assay in which we clearly showed that local enrichment of this SPION-drug conjugate with a magnet allows site-specific therapeutic effects.
authors: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany.
authors: Jan
authors: J
authors: Zaloga
authors: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany.
authors: Marina
authors: M
authors: Pöttler
authors: Institute of Cell Biology, Histology, and Embryology, Medical University of Graz, Graz, Austria; Center for Medical Research, Medical University of Graz, Graz, Austria.
authors: Gerd
authors: G
authors: Leitinger
authors: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany.
authors: Ralf P
authors: RP
authors: Friedrich
authors: Center for Medical Research, Medical University of Graz, Graz, Austria; Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
authors: Gunter
authors: G
authors: Almer
authors: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany.
authors: Stefan
authors: S
authors: Lyer
authors: Pharmacy Department, Erlangen University Hospital, Erlangen, Germany.
authors: Eva
authors: E
authors: Baum
authors: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany.
authors: Rainer
authors: R
authors: Tietze
authors: Pharmacy Department, Erlangen University Hospital, Erlangen, Germany.
authors: Ralph
authors: R
authors: Heimke-Brinck
authors: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
authors: Harald
authors: H
authors: Mangge
authors: Pharmacy Department, Erlangen University Hospital, Erlangen, Germany.
authors: Frank
authors: F
authors: Dörje
authors: Division of Pharmaceutics, Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany.
authors: Geoffrey
authors: G
authors: Lee
authors: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Erlangen, Germany. Electronic address: c.alexiou@web.de.
authors: Christoph
authors: C
authors: Alexiou
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D016503:Drug Delivery Systems; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D008280:Magnetics / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods; D008942:Mitoxantrone / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D012709:Serum Albumin; C000499:ferric oxide; D008942:Mitoxantrone
keywords: Good manufacturing practice; Human serum albumin; Magnetic drug targeting; Superparamagnetic iron oxide nanoparticles