title: An in vivo analysis of Miromesh--a novel porcine liver prosthetic created by perfusion decellularization.
abstract: BACKGROUND
  Bioprosthetics derived from human or porcine dermis and intestinal submucosa have dense, homogenous, aporous collagen structures that potentially limit cellular penetration, undermining the theoretical benefit of a "natural" collagen scaffold. We hypothesized that Miromesh-a novel prosthetic derived from porcine liver by perfusion decellularization-provides a more optimal matrix for tissue ingrowth.
  
  
  METHODS
  Thirty rats underwent survival surgery that constituted the creation of a 4 × 1 cm abdominal defect and simultaneous bridged repair. Twenty rats were bridged with Miromesh, and 10 rats were bridged with non-cross-linked porcine dermis (Strattice). Ten Miromesh and all 10 Strattice were rinsed in vancomycin solution and inoculated with 10(4) colony-forming units of green fluorescent protein-labeled Staphylococcus aureus (GFP-SA) after implantation. Ten Miromesh controls were neither soaked nor inoculated. No animals received systemic antibiotics. All animals were euthanized at 90 d and underwent an examination of their gross appearance before being sectioned for quantitative bacterial culture and histologic grading. A pathologist scored specimens (0-4) for cellular infiltration, acute inflammation, chronic inflammation, granulation tissue, foreign body reaction, and fibrous capsule formation.
  
  
  RESULTS
  All but one rat repaired with Strattice survived until the 90-d euthanization. All quantitative bacterial cultures for inoculated specimens were negative for GFP-SA. Of nine Strattice explants, none received a cellular infiltration score >0, consistent with a poor tissue-mesh interface observed grossly. Of 10 Miromesh explants also inoculated with GFP-SA, seven of 10 demonstrated cellular infiltration with an average score of +2.7 ± 0.8, whereas sterile Miromesh implants received an average score of 0.8 ± 1.0. Two inoculated Miromesh implants demonstrated acute inflammation and infection on histology.
  
  
  CONCLUSIONS
  A prosthetic generated from porcine liver by perfusion decellularization provides a matrix for superior cellular infiltration compared with non-cross-linked porcine dermis.
authors: Department of General Surgery, Case Comprehensive Hernia Center, University Hospitals Case Medical Center, Cleveland, Ohio. Electronic address: Clayton.Petro@UHhospitals.org.
authors: Clayton C
authors: CC
authors: Petro
authors: Department of General Surgery, Case Comprehensive Hernia Center, University Hospitals Case Medical Center, Cleveland, Ohio.
authors: Ajita S
authors: AS
authors: Prabhu
authors: Department of General Surgery, Case Comprehensive Hernia Center, University Hospitals Case Medical Center, Cleveland, Ohio.
authors: Lijia
authors: L
authors: Liu
authors: Department of General Surgery, Case Comprehensive Hernia Center, University Hospitals Case Medical Center, Cleveland, Ohio.
authors: Arnab
authors: A
authors: Majumder
authors: Department of Pathology, University Hospitals Case Medical Center, Cleveland, Ohio.
authors: James M
authors: JM
authors: Anderson
authors: Department of General Surgery, Cleveland Clinic Comprehensive Hernia Center, Cleveland Clinic, Cleveland, Ohio.
authors: Michael J
authors: MJ
authors: Rosen
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D008099:Liver; D017207:Rats, Sprague-Dawley; D013526:Surgical Mesh; D013552:Swine
keywords: Biologic; Bioprosthetic; Cellular infiltration; Contamination; Hernia; Incisional; Mesh; Perfusion decellularization; Ventral