title: Simple solutions to false results with plate/slide agglutination tests in diagnosis of infectious diseases of man and animals.
abstract: We have developed a new Superagglutination test for serodiagnosis of infectious diseases. It differs from conventional plate/slide agglutination tests (PAT/SAT) by three additional steps: prior staining of serum antibody by adding a dye and addition of diluted biotinylated antiglobulin and avidin in sequence after mixing the antigen with the test serum. The new steps circumvent the problems of false positive and false negative results of PAT/SAT. In serodiagnosis of brucellosis, Superagglutination test had higher positive predictive value and specificity than Rose Bengal Plate Test (RBPT) and Standard Tube Agglutination Test (STAT) and higher negative predictive value and sensitivity than RBPT, STAT, ELISA and Complement Fixation Test (CFT).•Superagglutination is a simple, accurate and economic screening test for infections.•More specificity, sensitivity, positive & negative predictive value than RBPT, STAT.•More sensitivity, negative predictive value than ELISA and Complement Fixation Test.
authors: Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev, Veterinary and Animal Sciences University (GADVASU), Ludhiana 141004, India.
authors: Hari Mohan
authors: HM
authors: Saxena
authors: Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev, Veterinary and Animal Sciences University (GADVASU), Ludhiana 141004, India.
authors: Shubhada
authors: S
authors: Chothe
authors: Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev, Veterinary and Animal Sciences University (GADVASU), Ludhiana 141004, India.
authors: Paviter
authors: P
authors: Kaur
keywords: Agglutination test; Coombs’ test; False negative; False positive; Rose Bengal Plate Test; Superagglutination; Superagglutination test