title: MAZ mediates the cross-talk between CT-1 and NOTCH1 signaling during gliogenesis.
abstract: Neurons and glia cells are differentiated from neural stem/progenitor cells (NSCs/NPCs) during brain development. Concomitant activation of JAK/STAT and NOTCH1 signaling is required for gliogenesis, a process to generate glia cells to ensure proper brain functions. NOTCH1 signaling is down-regulated during neurogenesis and up-regulated during gliogenesis. However, the underlying mechanism remains elusive. We report here that cardiotrophin-1 (CT-1) activates NOTCH1 signaling through the up-regulation of ADAM10, a rate-limiting factor of NOTCH1 signaling activation. We found that a transcriptional factor, Myc-associated zinc finger protein (MAZ), plays an important role in ADAM10 transcription in response to CT-1 in NPCs. MAZ knockdown inhibits CT-1 stimulated gliogenesis and it can be rescued by over-expressing human NICD. Our results provide a link between NOTCH1 activation and neuronal secreted CT-1, suggesting that CT-1 plays an important role in ensuring the coordinated activation of NOTCH1 signaling during gliogenesis.
authors: State Key Laboratory of Genetic Engineering and Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University.
authors: Bin
authors: B
authors: Liu
authors: State Key Laboratory of Genetic Engineering and Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University.|The Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, China, 200433.
authors: Anyun
authors: A
authors: Ma
authors: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China 100101.
authors: Feng
authors: F
authors: Zhang
authors: State Key Laboratory of Genetic Engineering and Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University.|The Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, China, 200433.
authors: Yumeng
authors: Y
authors: Wang
authors: State Key Laboratory of Genetic Engineering and Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University.|The Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, China, 200433.
authors: Zengmin
authors: Z
authors: Li
authors: State Key Laboratory of Genetic Engineering and Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University.
authors: Qingyu
authors: Q
authors: Li
authors: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China 100101.|Innovation Center for International Cooperation of Genetics and Development, Fudan University, Shanghai, China, 200433.
authors: Zhiheng
authors: Z
authors: Xu
authors: State Key Laboratory of Genetic Engineering and Ministry of Education (MOE) Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University.|The Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, China, 200433.|Innovation Center for International Cooperation of Genetics and Development, Fudan University, Shanghai, China, 200433.
authors: Yufang
authors: Y
authors: Zheng
mesh_terms: D000072197:ADAM10 Protein / Q000096:biosynthesis / Q000235:genetics; D053829:Amyloid Precursor Protein Secretases / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; D014157:Transcription Factors; C076035:c-MYC-associated zinc finger protein; C092073:cardiotrophin 1; D053829:Amyloid Precursor Protein Secretases; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human