title: Survival of breast cancer patients according to changes in expression of selected markers between primary tumor and lymph node metastases.
abstract: BACKGROUND
  The differences between primary and metastatic tumor cells might be important for treatment selection and prognostication.
  
  
  METHODS
  Expression of ER, PR, HER2, CK5/6, EGFR, Ki-67, Ep-CAM, P-cadherin, CD24, CD44, ALDH was assessed immunohistochemically in primary tumor (T) and corresponding synchronous nodal metastases (LNM) in 156 invasive ductal breast cancer patients (T ≥1, N ≥1, M0).
  
  
  RESULTS
  Independent negative prognostic factors for disease-free survival were pN3, ALDH immunopositivity in LNM, nonluminal A subtype in LNM, reduction of Ep-CAM expression in LNM, lack of changes or enhancement of CK5/6 and ALDH expression in LNM.
  
  
  CONCLUSIONS
  Our results suggest that in some cases expression of markers in lymph node metastases might bring additional prognostic information to that obtained from primary tumor.
authors: Department of Applied Radiobiology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Agnieszka
authors: A
authors: Adamczyk
authors: Department of Applied Radiobiology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Joanna
authors: J
authors: Niemiec
authors: Department of Tumor Pathology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Aleksandra
authors: A
authors: Ambicka
authors: Clinic of Oncology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Anna
authors: A
authors: Mucha-Małecka
authors: Department of Tumor Pathology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Janusz
authors: J
authors: Ryś
authors: Department of Surgical Oncology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Jerzy
authors: J
authors: Mituś
authors: Department of Surgical Oncology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Wojciech M
authors: WM
authors: Wysocki
authors: Department of Applied Radiobiology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Anna
authors: A
authors: Cichocka
authors: Clinic of Oncology, Maria Sklodowska-Curie Memorial Cancer Centre & Institute of Oncology, Cracow Branch, 31-115 Cracow, Garncarska 11, Poland.
authors: Jerzy
authors: J
authors: Jakubowicz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D016016:Proportional Hazards Models; D000071036:Sentinel Lymph Node / Q000378:metabolism*; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: breast carcinoma; changes in proteins expression; lymph node metastases; primary tumor