title: The first report on coagulation and phospholipase A2 activities of Persian Gulf lionfish, Pterois russelli, an Iranian venomous fish.
abstract: Pterois russelli is a venomous fish belonging to scorpionidae family. Regarding to high significance value for tracing potential therapeutic molecules and special agents from venomous marine creatures, the present study was aimed to characterization of the Persian Gulf lionfish venom. Proteolytic, phospholipase, hemolytic, coagulation, edematogenic and dermonecrotic activities were determined for extracted venom. The LD50 of P. russelli venom was determined by intravenous injection in white Balb/c mice. Phospholipase A2 activity was recorded at 20 μg of total venom. Coagulation activity on human plasma was shown by Prothrombin Time (PT) and activated Partial Thromboplastin Time (APTT) assays and coagulation visualized after 7 and 14 s respectively for 60 μg of crude venom. LD50 was calculated as 10.5 mg/kg. SDS-PAGE revealed the presence of major and minor protein bands between 6 and 205 kDa. Different amounts of crude venom ranged from 1.87 to 30 μg showed proteolytic activity on casein. The highest edematic activity was detected at 20 μg. Our findings showed that the edematic activity was dose dependent and persisted for 48 h after injection. The crude venom did not induce dermonecrotic activity on rabbit skin and showed no hemolytic activity on human, mouse and rabbit erythrocytes. This is the first report for phospholipase A2 and coagulation activity in venomous fish and venomous marine animals respectively. Proteolytic activity of P. russelli venom is in accordance with the other genara of scorpionidae family. According to venom activity on intrinsic and extrinsic coagulation pathways, lionfish venom would be contained an interesting pharmaceutical agent. This study is pending to further characterization of phospholipase A2, coagulation, and protease activities and also in vivo activity on animal model of surface and internal bleeding.
authors: Department of Marine Biology, Faculty of Marine Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors: Bahareh
authors: B
authors: Memar
authors: Department of Marine Biology, Faculty of Marine Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran; Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran. Electronic address: shahlajamili45@yahoo.com.
authors: Shahla
authors: S
authors: Jamili
authors: Biotechnology Research Center, Medical Biotechnology Dept., Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran. Electronic address: shahbazzadeh@pasteur.ac.ir.
authors: Delavar
authors: D
authors: Shahbazzadeh
authors: Biotechnology Research Center, Medical Biotechnology Dept., Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran. Electronic address: k_bagheri@pasteur.ac.ir.
authors: Kamran Pooshang
authors: KP
authors: Bagheri
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D004487:Edema; D004591:Electrophoresis, Polyacrylamide Gel; D005397:Fish Venoms / Q000737:chemistry / Q000633:toxicity*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D019156:Indian Ocean; D007492:Iran; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D010473:Perciformes / Q000502:physiology*; D054467:Phospholipases A2 / Q000378:metabolism*; D011817:Rabbits; D012867:Skin / Q000187:drug effects
chemical_list: D005397:Fish Venoms; D054467:Phospholipases A2
keywords: Coagulation; Lionfish; Phospholipase A2; Pterois russelli; The Persian Gulf; Venomous marine fish