title: Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57.
abstract: There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.
authors: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.|Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.
authors: Mengjie
authors: M
authors: Wang
authors: Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.|Stanley Ho Center for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.|Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
authors: Qi
authors: Q
authors: Dong
authors: Stanley Ho Center for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.
authors: Hua
authors: H
authors: Wang
authors: Shenzhen Center for Disease Control and Prevention (Shenzhen CDC), Shenzhen, China.
authors: Yaqing
authors: Y
authors: He
authors: Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
authors: Ying
authors: Y
authors: Chen
authors: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: Hong
authors: H
authors: Zhang
authors: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: Rong
authors: R
authors: Wu
authors: Institute of Infectious Diseases, The 3rd Peoples' Hospital of Shenzhen, Shenzhen, China.
authors: Xinchun
authors: X
authors: Chen
authors: Institute of Infectious Diseases, The 3rd Peoples' Hospital of Shenzhen, Shenzhen, China.
authors: Boping
authors: B
authors: Zhou
authors: College of Letter and Sciences, University of California at Berkeley, Berkeley, CA, USA.
authors: Jason
authors: J
authors: He
authors: Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.|Stanley Ho Center for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.
authors: Hsiang-Fu
authors: HF
authors: Kung
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
authors: Canhua
authors: C
authors: Huang
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
authors: Yuquan
authors: Y
authors: Wei
authors: School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China.
authors: Jian-dong
authors: JD
authors: Huang
authors: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors: Hongxi
authors: H
authors: Xu
authors: Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
authors: Ming-Liang
authors: ML
authors: He
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D029821:Enterovirus A, Human / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D029760:Garcinia / Q000737:chemistry*; D016679:Genome, Viral; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D019704:Protein Disulfide-Isomerases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012208:Rhabdomyosarcoma / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013729:Terpenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012367:RNA, Viral; D013729:Terpenes; D014764:Viral Proteins; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human
keywords: ERp57; Oblongifolin M; antiviral; enterovirus 71