title: Epigenetic Activation of Antibacterial Property of an Endophytic Streptomyces coelicolor Strain AZRA 37 and Identification of the Induced Protein Using MALDI TOF MS/MS.
abstract: The endophytic Streptomyces coelicolor strain AZRA 37 was isolated from the surface sterilized root of Azadirachta indica A. Juss., commonly known as neem plant in India. Since only a few reports are available regarding epigenetic modulations of microbial entities, S. coelicolor was treated with different concentrations of 5-azacytidine for this purpose and evaluated for its antibacterial potential against five human pathogenic bacteria (Aeromonas hydrophila IMS/GN11, Enterococcus faecalis IMS/GN7, Salmonella typhi MTCC 3216, Shigella flexneri ATCC 12022 and Staphylococcus aureus ATCC 25923). The crude extract obtained from cultures treated with 25 μM concentration of 5-azacytidine, was found effective against all five pathogenic bacteria tested while the untreated control was only active against 3 pathogenic bacteria. HPLC analysis of crude compounds from treated cultures showed a greater number of compounds than that of the control. Extraction of whole cell protein and its SDS PAGE analysis showed an additional major protein band in 25 μM 5-azacytidine treated culture and MALDI TOF MS/MS analysis revealed that this protein belongs to the porin family.
authors: Mycopathology and Microbial Technology Laboratory, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
authors: Jitendra
authors: J
authors: Kumar
authors: Mycopathology and Microbial Technology Laboratory, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
authors: Vijay K
authors: VK
authors: Sharma
authors: Mycopathology and Microbial Technology Laboratory, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
authors: Dheeraj K
authors: DK
authors: Singh
authors: Mycopathology and Microbial Technology Laboratory, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
authors: Ashish
authors: A
authors: Mishra
authors: Dept of Botany, Visva-Bharati, Shantiniketan, Bolpur, India.
authors: Surendra K
authors: SK
authors: Gond
authors: Mycopathology and Microbial Technology Laboratory, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
authors: Satish K
authors: SK
authors: Verma
authors: Dept of Botany, Budhha PG College, Kushinagar, India.
authors: Anuj
authors: A
authors: Kumar
authors: Mycopathology and Microbial Technology Laboratory, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.
authors: Ravindra Nath
authors: RN
authors: Kharwar
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D001374:Azacitidine / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D044127:Epigenesis, Genetic; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D064210:Secondary Metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D048372:Streptomyces coelicolor / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D001374:Azacitidine