title: Evaluation of 1100 couples with recurrent pregnancy loss using conventional cytogenetic, PGD, and PGS: hype or hope.
abstract: Recurrent pregnancy loss (RPL) is an important clinical problem, mostly resulting from chromosomal or genetic defects, while in 30-60% of cases, it is idiopathic. The aim of this study is to evaluate the frequency and types of chromosomal abnormalities, also pre-implantation genetic diagnosis (PGD) and pre-implantation genetic screening (PGS) outcomes among Iranian couples with RPL. This retrospective study was conducted on 1100 Iranian couples (2200 individuals) with RPL referred to Royan Institute between 2008 and 2014. Karyotyping had been performed using standard cytogenetic techniques. PGD results of RPL patients with abnormal karyotypes and PGS results of RPL patients with normal karyotypes were also analyzed. The frequency of chromosomal abnormalities in these patients was 4.95%. Women demonstrated more abnormalities (6.82%) in comparison to men (3.09%). The successful rate of pregnancy after PGD and PGS was 52 and 18.64%, respectively. The observation of 4.95% chromosomal abnormalities among the patients with RPL could support this hypothesis that there is a direct relationship between chromosomal abnormalities and RPL. More than half of the patients who underwent PGD had successful pregnancy; therefore, this approach can improve the success rate of pregnancy in them. The results of PGS cycles showed that this technique could increase the live birth rate in RPL patients.
authors: a Department of Genetics , Tehran Medical Sciences Branch, Islamic Azad University , Tehran , Iran .|b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.
authors: Kamelia
authors: K
authors: Farahmand
authors: b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.
authors: Hamid
authors: H
authors: Kalantari
authors: b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.
authors: Mostafa
authors: M
authors: Fakhri
authors: b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.|c Iranian Biological Resource Center (IBRC), ACECR , Tehran , Iran.
authors: Abolhasan Shahzadeh
authors: AS
authors: Fazeli
authors: b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.
authors: Shabnam Zari
authors: SZ
authors: Moradi
authors: b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.
authors: Navid
authors: N
authors: Almadani
authors: a Department of Genetics , Tehran Medical Sciences Branch, Islamic Azad University , Tehran , Iran .
authors: Mehrdad
authors: M
authors: Hashemi
authors: b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.
authors: Hamid
authors: H
authors: Gourabi
authors: b Department of Genetics at Reproductive Biomedicine Research Center , Royan Institute for Reproductive Biomedicine, ACECR , Tehran , Iran , and.
authors: Anahita
authors: A
authors: Mohseni-Meybodi
mesh_terms: D000026:Abortion, Habitual / Q000175:diagnosis* / Q000453:epidemiology*; D000328:Adult; D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D005260:Female; D005820:Genetic Testing / Q000706:statistics & numerical data*; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000706:statistics & numerical data*
keywords: Chromosomal abnormalities; cytogenetic; pre-implantation genetic diagnosis; pre-implantation genetic screening; recurrent pregnancy loss