title: Optical characterization of epidermal cells and their relationship to DNA recovery from touch samples.
abstract: The goal of this study was to investigate the relative contributions of different cellular and genetic components to biological samples created by touch or contact with a surface - one of the most challenging forms of forensic evidence. Touch samples were generated by having individuals hold an object for five minutes and analyzed for quantity of intact epidermal cells, extracellular DNA, and DNA from pelleted cell material after elution from the collection swab. Comparisons were made between samples where individuals had washed their hands immediately prior to handling and those where hand washing was not controlled. The vast majority (84-100%) of DNA detected in these touch samples was extracellular and was uncorrelated to the number of epidermal cells detected. Although little to no extracellular or cell pellet-associated DNA was detected when individuals washed their hands prior to substrate handling, we found that a significant number of epidermal cells (between ~5x10 (3) and ~1x10 (5)) could still be recovered from these samples, suggesting that other types of biological information may be present even when no amplifiable nuclear DNA is present. These results help to elucidate the biological context for touch samples and characterize factors that may contribute to patterns of transfer and persistence of genetic material in forensic evidence.
authors: Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, 23284, USA.
authors: Cristina E
authors: CE
authors: Stanciu
authors: Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, 23284, USA.
authors: M Katherine
authors: MK
authors: Philpott
authors: Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, 23284, USA.
authors: Ye Jin
authors: YJ
authors: Kwon
authors: Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, 23284, USA.
authors: Eduardo E
authors: EE
authors: Bustamante
authors: Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, 23284, USA.
authors: Christopher J
authors: CJ
authors: Ehrhardt
keywords: epidermal cell; extracellular DNA; flow cytometry; forensic science; touch DNA