title: Synthesis of Pyridone-based Nucleoside Analogues as Substrates or Inhibitors of DNA Polymerases.
abstract: The synthesis and characterization of novel acyclic and cyclic pyridone-based nucleosides and nucleotides is described. In total, seven nucleosides and four nucleotides were synthesized. None of the tested nucleosides showed inhibitory properties against Klenow exo- polymerase and M.MuLV and HIV-1 reverse transcriptases. The nucleotides containing 4-chloro- and 4-bromo-2-pyridone as a nucleobase were accepted by the Klenow fragment, but at the expense of fidelity and extension efficiency.
authors: a Department of Molecular Microbiology and Biotechnology , Institute of Biochemistry, Vilnius University , Mokslininkų 12, Vilnius LT , Lithuania.
authors: Daiva
authors: D
authors: Tauraitė
authors: a Department of Molecular Microbiology and Biotechnology , Institute of Biochemistry, Vilnius University , Mokslininkų 12, Vilnius LT , Lithuania.
authors: Rytis
authors: R
authors: Ražanas
authors: b Faculty of Natural Sciences , Vilnius University , MK Čiurlionio 21/27, Vilnius LT , Lithuania.
authors: Algirdas
authors: A
authors: Mikalkėnas
authors: a Department of Molecular Microbiology and Biotechnology , Institute of Biochemistry, Vilnius University , Mokslininkų 12, Vilnius LT , Lithuania.|b Faculty of Natural Sciences , Vilnius University , MK Čiurlionio 21/27, Vilnius LT , Lithuania.
authors: Saulius
authors: S
authors: Serva
authors: a Department of Molecular Microbiology and Biotechnology , Institute of Biochemistry, Vilnius University , Mokslininkų 12, Vilnius LT , Lithuania.
authors: Rolandas
authors: R
authors: Meškys
mesh_terms: D001419:Bacteria; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors / Q000378:metabolism; D019384:Nucleic Acid Synthesis Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D009705:Nucleosides / Q000138:chemical synthesis* / Q000494:pharmacology; D009711:Nucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D011728:Pyridones / Q000138:chemical synthesis* / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D009705:Nucleosides; D009711:Nucleotides; D011728:Pyridones; D014764:Viral Proteins; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I
keywords: HIV-1 RT; Klenow exo-; M.MuLV; Pyridone nucleobase; modified nucleotides; nucleoside analogues