title: Xenobiotic Compounds Degradation by Heterologous Expression of a Trametes sanguineus Laccase in Trichoderma atroviride.
abstract: Fungal laccases are enzymes that have been studied because of their ability to decolorize and detoxify effluents; they are also used in paper bleaching, synthesis of polymers, bioremediation, etc. In this work we were able to express a laccase from Trametes (Pycnoporus) sanguineus in the filamentous fungus Trichoderma atroviride. For this purpose, a transformation vector was designed to integrate the gene of interest in an intergenic locus near the blu17 terminator region. Although monosporic selection was still necessary, stable integration at the desired locus was achieved. The native signal peptide from T. sanguineus laccase was successful to secrete the recombinant protein into the culture medium. The purified, heterologously expressed laccase maintained similar properties to those observed in the native enzyme (Km and kcat and kcat/km values for ABTS, thermostability, substrate range, pH optimum, etc). To determine the bioremediation potential of this modified strain, the laccase-overexpressing Trichoderma strain was used to remove xenobiotic compounds. Phenolic compounds present in industrial wastewater and bisphenol A (an endocrine disruptor) from the culture medium were more efficiently removed by this modified strain than with the wild type. In addition, the heterologously expressed laccase was able to decolorize different dyes as well as remove benzo[α]pyrene and phenanthrene in vitro, showing its potential for xenobiotic compound degradation.
authors: Facultad de Ciencias, Universidad Autónoma del estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Mor, México.|Centro de Investigación en Biotecnología, Universidad Autónoma del estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.
authors: Edgar
authors: E
authors: Balcázar-López
authors: Facultad de Ciencias, Universidad Autónoma del estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Mor, México.|Centro de Investigación en Biotecnología, Universidad Autónoma del estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.
authors: Luz Helena
authors: LH
authors: Méndez-Lorenzo
authors: Facultad de Ciencias, Universidad Autónoma del estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Mor, México.|Centro de Investigación en Biotecnología, Universidad Autónoma del estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.
authors: Ramón Alberto
authors: RA
authors: Batista-García
authors: Unidad de Microbiología Básica y Aplicada, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Anillo vial fray Junípero Serra km 8 s/n C.P. 76000, Santiago de Qro, México.
authors: Ulises
authors: U
authors: Esquivel-Naranjo
authors: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Mor, México.
authors: Marcela
authors: M
authors: Ayala
authors: Department of Civil Engineering, Université de Sherbrooke, 2500 Boulevard de l'Université, Sherbrooke, Québec J1K 2R1, Canada.|Bioprocess laboratory, Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamil Nadu, India.
authors: Vaidyanathan Vinoth
authors: VV
authors: Kumar
authors: Department of Civil Engineering, Université de Sherbrooke, 2500 Boulevard de l'Université, Sherbrooke, Québec J1K 2R1, Canada.
authors: Olivier
authors: O
authors: Savary
authors: Department of Civil Engineering, Université de Sherbrooke, 2500 Boulevard de l'Université, Sherbrooke, Québec J1K 2R1, Canada.
authors: Hubert
authors: H
authors: Cabana
authors: Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Campus Guanajuato, Km. 9.6 Libramiento Norte, Carretera Irapuato-León, 36821 Irapuato, México.
authors: Alfredo
authors: A
authors: Herrera-Estrella
authors: Centro de Investigación en Biotecnología, Universidad Autónoma del estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.
authors: Jorge Luis
authors: JL
authors: Folch-Mallol
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D007700:Kinetics; D042845:Laccase / Q000235:genetics*; D011994:Recombinant Proteins; D013379:Substrate Specificity; D055454:Trametes / Q000201:enzymology / Q000235:genetics*; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*; D015262:Xenobiotics / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; D015262:Xenobiotics; D042845:Laccase