title: Blastocysts derivation from somatic cell fusion with premature oocytes (prematuration somatic cell fusion).
abstract: This study was undertaken to investigate the development of immature oocytes after their fusion with male somatic cells expressing red fluorescence protein (RFP). RFP-expressing cells were fused with immature oocytes, matured in vitro and then parthenogenetically activated. Somatic nuclei showed spindle formation, 1st polar body extrusion after in vitro maturation and protruded the 2nd polar body after parthenogenetic activation. RFP was expressed in the resultant embryos; two-cell stage and blastocysts. Chromosomal analysis showed aneuploidy in 81.82% of the resulting blastocysts while 18.18% of the resulting blastocysts were diploid. Among eight RFP-expressing blastocysts, Xist mRNAs was detected in six while Sry mRNA was detected in only one blastocyst. We propose "prematuration somatic cell fusion" as an approach to generate embryos using somatic cells instead of spermatozoa. The current approach, if improved, would assist production of embryos for couples where the male partner is sterile, however, genetic and chromosomal analysis of the resultant embryos are required before transfer to the mothers.
authors: Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt.
authors: Islam M
authors: http://orcid.org/0000-0002-7633-730X
authors: IM
authors: Saadeldin
authors: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Korea.
authors: Candrani
authors: C
authors: Khoirinaya
authors: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Korea.
authors: Su Jin
authors: SJ
authors: Kim
authors: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Korea.
authors: Joon Ho
authors: JH
authors: Moon
authors: Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.
authors: Essam
authors: E
authors: Almadaly
authors: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and the Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Korea.|Designed Animal and Transplantation, Institute of Green Bio Science Technology, Seoul National University, Seoul, 232-916, Pyeongchang, Korea.
authors: Byeong Chun
authors: BC
authors: Lee
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002459:Cell Fusion; D005260:Female; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D010312:Parthenogenesis; D012333:RNA, Messenger / Q000378:metabolism; D051876:Sex-Determining Region Y Protein / Q000378:metabolism; D012756:Sheep; D013552:Swine
chemical_list: D012333:RNA, Messenger; D051876:Sex-Determining Region Y Protein
keywords: aneuploidy; haploidization; immature oocyte; infertility; sterility