title: Effect of α-Hederin on IL-2 and IL-17 mRNA and miRNA-133a Levels in Lungs of Ovalbumin-Sensitized Male Rats.
abstract: α-hederin, a saponin that is a major constituent of English Ivy (Hedera helix) is effective in the treatment of asthma. In the present study, the effect of α-hederin on lung tissue pathology and the levels of the inflammatory mediators; IL-2 mRNA, IL-17 mRNA, and MicroRNAs (miRNA)-133a was evaluated in a rat ovalbumin (OVA)-sensitized model of asthma. Rats were divided randomly into control (C), OVA-sensitized (S), OVA-sensitized pretreated with the antioxidant, thymoquinone (3 mg/kg, S + TQ) or OVA-sensitized pretreated with α-hederin (0.02 mg/kg, S + AH) groups. Levels of IL-2 and IL-17 mRNA were higher in the OVA-sensitized group than controls while the level of miRNA-133a gene expression was lower. IL-2 mRNA and miRNA-133a gene expression in the S + TQ group was higher than in the control and OVA-sensitized groups while the level of IL-17 mRNA in the S + TQ group was lower than in the OVA-sensitized group. Pretreatment with α-hederin decreased IL-17 mRNA levels and increased miRNA-133a gene expression compared with OVA-sensitized animals. All pathological changes in pretreated groups were lower than the OVA-sensitized group. These results showed a beneficial effect of α-hederin in OVA-sensitized rats, suggesting that α-hederin affects the IL-2 and IL-17 secretion pathways, altering miRNA-133a expression.
authors: Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Hadi
authors: H
authors: Ebrahimi
authors: Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Maryam
authors: M
authors: Fallahi
authors: Department of Molecular Medicine, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Amir Mahdi
authors: AM
authors: Khamaneh
authors: Department of Basic Sciences, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
authors: Mohammad Ali
authors: MA
authors: Ebrahimi Saadatlou
authors: Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.|Department of Physiology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors: Saeideh
authors: S
authors: Saadat
authors: Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors: Rana
authors: R
authors: Keyhanmanesh
mesh_terms: D000485:Allergens; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D001249:Asthma / Q000378:metabolism* / Q000473:pathology; D020381:Interleukin-17 / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010047:Ovalbumin; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*
chemical_list: D000485:Allergens; D018927:Anti-Asthmatic Agents; D020381:Interleukin-17; D007376:Interleukin-2; C549446:MIRN133 microRNA, rat; D035683:MicroRNAs; D012333:RNA, Messenger; D012503:Saponins; C010177:beta-hederin; D009828:Oleanolic Acid; D010047:Ovalbumin
keywords: IL17 mRNA; IL2 mRNA; asthma; lung pathology; miR133a; α -hederin