title: Gold Nanorods as Colorful Chromogenic Substrates for Semiquantitative Detection of Nucleic Acids, Proteins, and Small Molecules with the Naked Eye.
abstract: Herein, we report for the first time a colorful chromogenic substrate, which displays vivid color responses in the presence of different concentration of analytes. Our investigation reveals that the selective shortening of gold nanorods (AuNRs) could generate a series of distinct colors that covers nearly the whole visible range from 400 to 760 nm. These vivid colors can be easily distinguished by the naked eye; as a result, the accuracy of visual inspection could be greatly improved. Next, we demonstrate the utility of AuNRs as multicolor chromogenic substrate to develop a number of colorimetric immunoassay methods, e.g., multicolor enzyme-linked immunosorbent assay (ELISA), multicolor competitive ELISA, and multicolor magnetic immunoassay (MIA). These methods allow us to visually quantify the concentration of a broad range of target molecules with the naked eye, and the obtained results are highly consistent with those state-of-the-art techniques that are tested by the sophisticated apparatus. These multicolor portable and cost-effective immunoassay approaches could be potentially useful for a number of applications, for example, in-home personal healthcare, on-site environmental monitoring, and food inspection in the field.
authors: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University , Fuzhou, 350116, China.
authors: Xiaoming
authors: X
authors: Ma
authors: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University , Fuzhou, 350116, China.|Fuqing Entry-Exit Inspection & Quarantine Bureau of P. R. China , Fuqing, 350300, China.
authors: Zhitao
authors: Z
authors: Chen
authors: Singapore Centre on Environment Life Sciences Engineering, Nanyang Technological University , 60 Nanyang Drive, SBS-01N-27, 637457, Singapore.
authors: Palanisamy
authors: P
authors: Kannan
authors: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University , Fuzhou, 350116, China.
authors: Zhenyu
authors: Z
authors: Lin
authors: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University , Fuzhou, 350116, China.
authors: Bin
authors: B
authors: Qiu
authors: Institute of Nanomedicine and Nanobiosensing, The Key Lab of Analysis and Detection Technology for Food Safety of the MOE and Fujian Province, College of Chemistry, Fuzhou University , Fuzhou, 350116, China.
authors: Longhua
authors: L
authors: Guo
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D009696:Nucleic Acids / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
chemical_list: D009696:Nucleic Acids; D011506:Proteins; D006046:Gold