title: Tinman Regulates NetrinB in the Cardioblasts of the Drosophila Dorsal Vessel.
abstract: Morphogenesis of the Drosophila dorsal vessel (DV) shares similarities with that of the vertebrate heart. Precursors line up at both sides of the embryo, migrate towards the midline and fuse to form a tubular structure. Guidance receptors and their ligands have been implicated in this process in vertebrates and invertebrates, as have been a series of evolutionarily conserved cardiogenic transcriptional regulators including Tinman, the Drosophila homolog of the transcription factor Nkx-2.5. NetrinB (NetB), a repulsive ligand for the Unc-5 receptor is required to preserve the dorsal vessel hollow. It localizes to the luminal space of the dorsal vessel but its source and its regulation is unknown. Here, using genetics together with in situ hybridization with single cell resolution, we show how tin is required for NetrinB expression in cardioblasts during DV tubulogenesis and sufficient to promote NetB transcription ectopically. We further identify a dorsal vessel-specific NetB enhancer and show that it is also regulated by tin in a similar fashion to NetB.
authors: Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland.|Institute of Neuroscience, Trinity College Dublin, Dublin, Ireland.
authors: Jamshid
authors: J
authors: Asadzadeh
authors: Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland.
authors: Niamh
authors: N
authors: Neligan
authors: Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, Rutgers The State University of New Jersey, Piscataway, New Jersey, United States of America.
authors: Sunita G
authors: SG
authors: Kramer
authors: Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland.|Institute of Neuroscience, Trinity College Dublin, Dublin, Ireland.
authors: Juan-Pablo
authors: JP
authors: Labrador
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000196:embryology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian / Q000098:blood supply* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D014157:Transcription Factors