title: Expression of TRAP1 in gastric cancer tissue and its correlation with malignant biology.
abstract: OBJECTIVE
  To study the expression of tumor necrosis factor receptor-associated protein 1 (TRAP1) in gastric cancer tissue and its correlation with malignant biology.
  
  
  METHODS
  Gastric cancer tissue and adjacent normal tissue were collected, and mRNA content and protein content of TRAP1 were detected; gastric cancer cell lines SGC7901, BGC823, AGS and MGC803 were cultured, and mRNA contents and protein contents of TRAP1, CyclinB1, CyclinD1, CyclinE, MMP-2 and VEGF were detected.
  
  
  RESULTS
  mRNA and protein expression levels of TRAP1 in gastric cancer tissue were significantly higher than those in adjacent normal tissue, and mRNA and protein expression levels of TRAP1 in gastric cancer tissue with muscularis and serosa infiltration, lymph node metastasis, distant organ metastasis and TNM Ⅲ/Ⅳ stage were significantly higher than those in gastric cancer tissue with mucosa and submucosa infiltration, non-lymph node metastasis, non-distant organ metastasis and TNM Ⅰ/Ⅱ stage. mRNA and protein expression levels of TRAP1, CyclinB1, CyclinD1, CyclinE, MMP-2 and VEGF in MGC803 were the highest, and mRNA and protein expression levels of TRAP1, CyclinB1, CyclinD1, CyclinE, MMP-2 and VEGF in SGC7901 were the lowest. mRNA and protein expression levels of TRAP1 in gastric cancer cell lines were positively correlated with mRNA and protein expression of CyclinB1, CyclinD1, CyclinE, MMP-2 and VEGF.
  
  
  CONCLUSIONS
  The expression of TRAP1 significantly increases in gastric cancer tissue; TRAP1 may regulate the malignant biology of cells by increasing the expression of CyclinB1, CyclinD1, CyclinE, MMP-2 and VEGF, thereby resulting in the occurrence and development of gastric cancer.
authors: Department of Gastrointestinal Surgery, Linyi People's Hospital, Linyi, Shandong 276000, China.
authors: Ping
authors: P
authors: Han
authors: Department of Gastrointestinal Surgery, Linyi People's Hospital, Linyi, Shandong 276000, China.
authors: Qing-Ling
authors: QL
authors: Wang
authors: Department of Respiratory Medicine, Linyi People's Hospital, Linyi, Shandong 276000, China. Electronic address: 37671997@qq.com.
authors: Xia
authors: X
authors: Zhang
keywords: Gastric cancer; Invasion; Malignant biology; Proliferation; TNF receptor-associated protein 1