title: Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR.
abstract: Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane-embedded ion channels where data analysis is commonly hindered by multi-spin effects.
authors: Centre of Magnetic Resonance, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.|Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.
authors: Silvia
authors: S
authors: Valera
authors: Centre of Magnetic Resonance, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.|Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.
authors: Katrin
authors: K
authors: Ackermann
authors: Centre of Magnetic Resonance, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.|Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.
authors: Christos
authors: http://orcid.org/0000-0002-4309-4858
authors: C
authors: Pliotas
authors: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.
authors: Hexian
authors: H
authors: Huang
authors: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK.
authors: James H
authors: http://orcid.org/0000-0001-6744-5061
authors: JH
authors: Naismith
authors: Centre of Magnetic Resonance, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK. beb2@st-andrews.ac.uk.|Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, KY16 9ST, UK. beb2@st-andrews.ac.uk.
authors: Bela E
authors: http://orcid.org/0000-0002-3384-271X
authors: BE
authors: Bode
keywords: EPR spectroscopy; biomimetic synthesis; deer; ion channels; membrane proteins; structural biology