title: Visualization of Anticancer Salmonella typhimurium Engineered for Remote Control of Therapeutic Proteins.
abstract: Tumor-targeting bacteria are studied for their ability to carry therapeutic molecules to tumors or, when designed as imaging probes, to visualize the infection pathway. The present protocol describes a method to achieve remote control of therapeutic gene expression in bacteria which are also engineered to visualize the therapeutic process. This strategy may increase the safety of bacteria used to deliver therapeutic genes to tumors in vivo.
authors: Laboratory of In Vivo Molecular Imaging, Department of Nuclear Medicine, Chonnam National University Medical School, 5, Hak 1 dong, Dong-gu, Gwangju, 501-746, South Korea.
authors: Vu H
authors: VH
authors: Nguyen
authors: Laboratory of In Vivo Molecular Imaging, Department of Nuclear Medicine, Chonnam National University Medical School, 5, Hak 1 dong, Dong-gu, Gwangju, 501-746, South Korea. jjmin@jnu.ac.kr.
authors: Jung-Joon
authors: JJ
authors: Min
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005818:Genetic Engineering; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism*; D019076:Transgenes; D051598:Whole Body Imaging / Q000379:methods
chemical_list: D003603:Cytotoxins
keywords: Bacteria; Cancer; Cytolysin A; Imaging; pBAD