title: Association between microRNA polymorphisms and chronic pancreatitis.
abstract: BACKGROUND
  MicroRNAs play important roles in the development and progression of many human diseases. mir-146a could significantly suppress the induction of proinflammatory cytokines IL-1β, IL-6, TNF-α, NF-κB and chemokine MCP-1, which might play important roles in chronic pancreatitis. This study was conducted to evaluate the association between mir-146a rs2910164, a functional polymorphism in the pre-mir-146a, and chronic pancreatitis risk.
  
  
  METHODS
  The rs2910164 genotypes were determined in 165 patients with chronic pancreatitis and 200 healthy controls who were frequency matched for age and gender. One single nucleotide polymorphism (rs2910164) was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RLFP).
  
  
  RESULTS
  The frequency of individuals who carried [G] allele was significantly higher in cases (62.7%) than in controls (53.7%, p = 0.015), which resulted in a statistically significant pathogenic effect associated with this variant allele (OR: 1.448, CI: 1.076-1.950; p = 0.015). The GC and GG genotypes showed strong and significant increased risk for complication of chronic pancreatitis (OR = 3.668, 95%CI = 1.233-10.916, p = 0.019; OR = 5.667, 95%CI = 1.852-17.336, p = 0.002). The individuals carrying G allele confer a lower expression level of mature mir-146a.
  
  
  CONCLUSIONS
  These findings suggest that the mir-146a rs2910164 may contribute to genetic susceptibility to chronic pancreatitis, and that mir-146a might be involved in chronic pancreatitis development.
authors: Department of Gastroenterology, The First Affiliated Hospital of He'nan University of Science and Technology, Luoyang 471000, China. Electronic address: janeywzz@163.com.
authors: Zhaozhi
authors: Z
authors: Wang
authors: Department of Nephrology, Chinese PLA General Hospital, Beijing 100853, China.
authors: Diangeng
authors: D
authors: Li
authors: Department of Gastroenterology, The First Affiliated Hospital of He'nan University of Science and Technology, Luoyang 471000, China.
authors: Jianjun
authors: J
authors: Jin
authors: Luoyang Oriental Hospital, Luoyang 471000, China.
authors: Qianshou
authors: Q
authors: Wang
authors: Department of Gastroenterology, The First Affiliated Hospital of He'nan University of Science and Technology, Luoyang 471000, China.
authors: Shuangqin
authors: S
authors: Zhao
authors: Department of Gastroenterology, The First Affiliated Hospital of He'nan University of Science and Technology, Luoyang 471000, China.
authors: Yanli
authors: Y
authors: Bai
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D050500:Pancreatitis, Chronic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D053682:Tendon Entrapment; D055815:Young Adult
chemical_list: D016207:Cytokines; C542542:MIRN146 microRNA, human; D035683:MicroRNAs
keywords: Chronic pancreatitis; Inflammatory disease; Pathophysiology; Polymorphism; Risk factor; mir-146a