title: Whole Transcriptome Analysis of Hypertension Induced Cardiac Injury Using Deep Sequencing.
abstract: OBJECTIVE
  Hypertension plays a critical role in the cardiac inflammation and injury. However, the mechanism of how hypertension causes the cardiac injury at a molecular level remains to be elucidated.
  
  
  METHODS
  RNA-Seq has been demonstrated to be an effective approach for transcriptome analysis, which is essential to reveal the molecular constituents of cells and tissues. In this study, we investigated the global molecular events associated with the mechanism of hypertension induced cardiac injury using RNA-Seq analysis.
  
  
  RESULTS
  Our results showed that totally 1,801 genes with different expression variations were identified after Ang II infusion at 1, 3 and 7 days. Go analysis showed that the top 5 high enrichment Go terms were response to stress, response to wounding, cellular component organization, cell activation and defense response. KEGG pathway analysis revealed the top 5 significantly overrepresented pathways were associated with ECM-receptor interaction, focal adhesion, protein digestion and absorption, phagosome and asthma. Moreover, protein-protein interaction network analysis indicated that ubiquitin C may play a key role in the processes of hypertension-induced cardiac injury.
  
  
  CONCLUSIONS
  Our study provides a comprehensive understanding of the transcriptome events in hypertension-induced cardiac pathology.
authors: Beijing Anzhen Hospital, Capital Medical University; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Collaborative Innovation Center for Cardiovascular Disorders; Beijing Institute of Heart, Lung & Blood Vessel Disease, Beijing, China.
authors: Tao-Tao
authors: TT
authors: Li
authors: Xiao-Yan
authors: XY
authors: Li
authors: Li-Xin
authors: LX
authors: Jia
authors: Jing
authors: J
authors: Zhang
authors: Wen-Mei
authors: WM
authors: Zhang
authors: Yu-Lin
authors: YL
authors: Li
authors: Yong-Fen
authors: YF
authors: Qi
authors: Jie
authors: J
authors: Du
mesh_terms: D000818:Animals; D005355:Fibrosis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006335:Heart Injuries / Q000150:complications / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D059014:High-Throughput Nucleotide Sequencing; D006973:Hypertension / Q000150:complications / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007249:Inflammation / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D060066:Protein Interaction Maps; D059467:Transcriptome