title: Two carbohydrate recognizing domains from Cycas revoluta leaf lectin show the distinct sugar-binding specificity-A unique mannooligosaccharide recognition by N-terminal domain.
abstract: Cycas revoluta leaf lectin (CRLL) of mannose-recognizing jacalin-related lectin (mJRL) has two tandem repeated carbohydrate recognition domains, and shows the characteristic sugar-binding specificity toward high mannose-glycans, compared with other mJRLs. We expressed the N-terminal domain and C-terminal domain (CRLL-N and CRLL-C) separately, to determine the fine sugar-binding specificity of each domain, using frontal affinity chromatography, glycan array and equilibrium dialysis. The specificity of CRLL toward high mannose was basically derived from CRLL-N, whereas CRLL-C had affinity for α1-6 extended mono-antennary complex-type glycans. Notably, the affinity of CRLL-N was most potent to one of three Man 8 glycans and Man 9 glycan, whereas the affinity of CRLL-C decreased with the increase in the number of extended α1-2 linked mannose residue. The recognition of the Man 8 glycans by CRLL-N has not been found for other mannose recognizing lectins. Glycan array reflected these specificities of the two domains. Furthermore, it was revealed by equilibrium dialysis method that the each domain had two sugar-binding sites, similar with Banlec, banana mannose-binding Jacalin-related lectin.
authors: Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan and.
authors: Michiko
authors: M
authors: Shimokawa
authors: Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan and.
authors: Tomokazu
authors: T
authors: Haraguchi
authors: Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan and.
authors: Yuji
authors: Y
authors: Minami
authors: Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan and k8021740@kadai.jp.
authors: Fumio
authors: F
authors: Yagi
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan.
authors: Keiko
authors: K
authors: Hiemori
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan.
authors: Hiroaki
authors: H
authors: Tateno
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan.
authors: Jun
authors: J
authors: Hirabayashi
mesh_terms: D029784:Cycas / Q000737:chemistry*; D037241:Mannose-Binding Lectins / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry*
chemical_list: D037241:Mannose-Binding Lectins; D037121:Plant Lectins
keywords: Cycas revoluta; Jacalin-related lectin; frontal affinity chromatography; glycan array; high-mannose glycan