title: Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil.
abstract: OBJECTIVE
  In the present work the acidophilic actinobacteria strain was used as a novel reducing agent for the cheap, green and single-step synthesis of nanostructure silver particles. Structural, morphological and optical properties of the synthesized nanoparticles have been characterized by spectroscopy, dynamic light scattering and electron microscopy approach. The antimicrobial activity of silver nanoparticles against clinical strains such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis and Salmonella infantis alone and in combination with antibiotics were studied.
  
  
  RESULTS
  The crystalline and stable biosynthesized silver nanoparticles ranged in size from 4 to 45 nm and were mostly spherical in shape being characterized evolving several analytical techniques. The bioAgNPs inhibited growth of most bacterial strains. The highest antimicrobial activity was observed against Ps. aeruginosa (10 mm), followed by Staph. aureus, B. subtilis and Pr. mirabilis (all 8 mm). The lower activity was noticed for E. coli and Kl. pneumoniae (6 and 2 mm, respectively). Moreover, the synergistic effect of bio(AgNPs) with various commercially available antibiotics was also evaluated. The most significant results were observed for bio(AgNPs) combined with tetracycline, kanamycin, ampicillin and neomycin, followed by streptomycin and gentamycin against E. coli, Salm. infantis and Kl. pneumoniae. The most resistant bacteria to commercial antibiotics was Pr. mirabilis.
  
  
  CONCLUSIONS
  The Streptacidiphilus sp. strain CGG11n isolated from acidic soil can be used to efficiently synthesize the bioactive nanoparticles using inexpensive substances in an eco-friendly and nontoxic manner. The present work provides helpful insight into the development of new antimicrobial agents with the synergistic enhancement of the antibacterial mechanism against pathogenic micro-organisms.
  
  
  CONCLUSIONS
  The synthesized silver bionanoparticles from Streptacidiphilus sp. strain CGG11n possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations.
authors: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors: V
authors: V
authors: Railean-Plugaru
authors: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors: P
authors: P
authors: Pomastowski
authors: Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
authors: M
authors: M
authors: Wypij
authors: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors: M
authors: M
authors: Szultka-Mlynska
authors: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors: K
authors: K
authors: Rafinska
authors: Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
authors: P
authors: P
authors: Golinska
authors: Department of Microbiology, Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland.
authors: H
authors: H
authors: Dahm
authors: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.|Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
authors: B
authors: B
authors: Buszewski
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D007711:Klebsiella pneumoniae / Q000187:drug effects; D053768:Metal Nanoparticles / Q000633:toxicity*; D008826:Microbial Sensitivity Tests; D028222:Picea / Q000382:microbiology*; D011513:Proteus mirabilis / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000378:metabolism* / Q000494:pharmacology; D012988:Soil Microbiology; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: Actinomycetes; antibacterial activity; antibiotics; bioproducts; soil