title: Development of a Targeted Gene-Delivery System Using Escherichia coli.
abstract: A gene-delivery system based on microbes is useful for development of targeted gene therapy of non-phagocytic cancer cells. Here, the feasibility of the delivery system is illustrated by targeted delivery of a transgene (i.e., eukaryotic GFP) by Escherichia coli to HER2/neu-positive cancer cells. An E. coli strain was engineered with surface display of the anti-HER2/neu affibody. To release the gene cargo, a programmed lysis system based on phage ϕX174 gene E was introduced into the E. coli strain. As a result, 3 % of HER2/neu-positive cells that were infected with engineered E. coli were able to express the GFP.
authors: Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road, 40402, Taichung, Taiwan. cjchiang@mail.cmu.edu.tw.
authors: Chung-Jen
authors: CJ
authors: Chiang
authors: Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, 40724, Taichung, Taiwan.
authors: Chih-Hsiang
authors: CH
authors: Chang
authors: Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, 40724, Taichung, Taiwan. ypchao@fcu.edu.tw.
authors: Yun-Peng
authors: YP
authors: Chao
authors: Department of Biological Science and Technology, China Medical University, No. 91, Hsueh-Shih Road, 40402, Taichung, Taiwan.
authors: Ming-Ching
authors: MC
authors: Kao
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006801:Humans; D018613:Microscopy, Confocal; D010957:Plasmids / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics; D019076:Transgenes
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases
keywords: Bacterial carrier; Bactofection; HER2/neu; Targeted delivery