title: Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto's thyroiditis and hypothyroidism.
abstract: The essential trace element selenium (Se) is required for thyroid hormone synthesis and metabolism. Selenoproteins contain selenocysteine and are responsible for biological functions of selenium. Glutathione peroxidase (GPx) is one of the major selenoproteins which protects the thyroid cells from oxidative damage. Selenoprotein P (SePP) is considered as the plasma selenium transporter to tissues. The aim of this study was to evaluate serum Se and SePP levels, and GPx activity in erythrocytes of children and adolescents with treated Hashimoto's thyroiditis, hypothyroidism, and normal subjects. Blood samples were collected from 32 patients with Hashimoto's thyroiditis, 20 with hypothyroidism, and 25 matched normal subjects. All the patients were under treatment with levothyroxine and at the time of analysis all of the thyroid function tests were normal. GPx enzyme activity was measured by spectrophotometry at 340 nm. Serum selenium levels were measured by high-resolution continuum source graphite furnace atomic absorption. SePP, TPOAb (anti-thyroid peroxidase antibody), and TgAb (anti-thyroglobulin antibody) were determined by ELISA kits. T4, T3, T3 uptake and TSH were also measured. Neither GPx activity nor SePP levels were significantly different in patients with Hashimoto's thyroiditis or hypothyroidism compared to normal subjects. Although GPx and SePP were both lower in patients with hypothyroidism compared to those with Hashimoto's thyroiditis and normal subjects but the difference was not significant. Serum Se levels also did not differ significantly in patients and normal subjects. We did not find any correlation between GPx or SePP with TPOAb or TgAb but SePP was significantly correlated with Se. Results show that in patients with Hashimoto's thyroiditis or hypothyroidism who have been under treatment with levothyroxine and have normal thyroid function tests, the GPx, SePP and Se levels are not significantly different.
authors: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Metabolic Disorders Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: nourbakhsh.m@iums.ac.ir.
authors: Mitra
authors: M
authors: Nourbakhsh
authors: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors: Fatemeh
authors: F
authors: Ahmadpour
authors: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors: Behnam
authors: B
authors: Chahardoli
authors: Department of Biochemistry, School of Medicine, Yazd University of Medical Sciences, Yazd, Iran.
authors: Zahra
authors: Z
authors: Malekpour-Dehkordi
authors: H.Aliasghar Hospital, Iran University of Medical Sciences, Tehran, Iran.
authors: Mona
authors: M
authors: Nourbakhsh
authors: Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors: Seyed Reza
authors: SR
authors: Hosseini-Fard
authors: Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Amirhossein
authors: A
authors: Doustimotlagh
authors: Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Abolfazl
authors: A
authors: Golestani
authors: Metabolic Disorders Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran; H.Aliasghar Hospital, Iran University of Medical Sciences, Tehran, Iran.
authors: Maryam
authors: M
authors: Razzaghy-Azar
mesh_terms: D000293:Adolescent; D002648:Child; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D050031:Hashimoto Disease / Q000097:blood*; D006801:Humans; D007037:Hypothyroidism / Q000097:blood*; D008297:Male; D012643:Selenium / Q000097:blood*; D051149:Selenoprotein P / Q000097:blood*; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology
chemical_list: D051149:Selenoprotein P; D005979:Glutathione Peroxidase; D012643:Selenium
keywords: Glutathione peroxidase; Hashimoto’s thyroiditis; Hypothyroidism; Selenium; Selenoprotein P; Thyroid