title: Chemical characterization of heteropolysaccharides from green and black teas (Camellia sinensis) and their anti-ulcer effect.
abstract: In order to obtain polysaccharides from green and black teas (Camellia sinensis), commercial leaves were submitted to infusion and then to alkaline extraction. The extracts were fractionated by freeze-thawing process, giving insoluble and soluble fractions. Complex arabinogalactan protein from the soluble fractions of both teas (GTPS and BTPS) were determined by methylation analysis and (1)H/(13)C-HSQC spectroscopy, showing a main chain of (1→3)-β-Galp, substituted at O-6 by (1→6)-linked β-Galp with side chains of α-Araf and terminal units of α-Araf, α-Fucp and α-Rhap. A highly branched heteroxylan from the insoluble fractions (GTPI and BTPI) showed in methylation analysis and (1)H/(13)C-HSQC spectroscopy the main chain of (1→4)-β-Xylp, substituted in O-3 by α-Araf, β-Galp and α-Glcp units. Evaluating their gastroprotective activity, the fractions containing the soluble heteropolysaccharides from green (GTPS) and black teas (BTPS) reduced the gastric lesions induced by ethanol. Furthermore, the fraction of insoluble heteropolysaccharides of green (GTPI) and black (BTPI) teas also protected the gastric mucosa. In addition, the maintenance of gastric mucus and reduced glutathione (GSH) levels was involved in the polysaccharides gastroprotection.
authors: Department of Biochemistry and Molecular Biology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil.
authors: Camila T
authors: CT
authors: Scoparo
authors: Instituto de Pesquisa Pelé Pequeno Príncipe, Faculdade Pequeno Príncipe, Curitiba, PR, Brazil.
authors: Lauro M
authors: LM
authors: Souza
authors: Department of Biology, Sector of Biological Sciences, State University of Centro-Oeste, Guarapuava, PR, Brazil.
authors: Nessana
authors: N
authors: Dartora
authors: Department of Biochemistry and Molecular Biology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil.
authors: Guilherme L
authors: GL
authors: Sassaki
authors: Department of Biochemistry and Molecular Biology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil.
authors: Arquimedes P
authors: AP
authors: Santana-Filho
authors: Department of Pharmacology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil.
authors: Maria Fernanda P
authors: MF
authors: Werner
authors: Department of Pharmacology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil.
authors: Débora G
authors: DG
authors: Borato
authors: Department of Pharmacology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil. Electronic address: crisbaggio@gmail.com.
authors: Cristiane H
authors: CH
authors: Baggio
authors: Department of Biochemistry and Molecular Biology, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, Brazil. Electronic address: iacomini@ufpr.br.
authors: Marcello
authors: M
authors: Iacomini
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D000431:Ethanol / Q000009:adverse effects; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013662:Tea / Q000737:chemistry*; D014456:Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D011134:Polysaccharides; D013662:Tea; D000431:Ethanol; D005978:Glutathione; D019342:Acetic Acid
keywords: Black tea; Gastroprotection; Green tea; Heteropolysaccharides