title: Selecting Optimal Peptides for Targeted Proteomic Experiments in Human Plasma Using In Vitro Synthesized Proteins as Analytical Standards.
abstract: In targeted proteomics, the development of robust methodologies is dependent upon the selection of a set of optimal peptides for each protein-of-interest. Unfortunately, predicting which peptides and respective product ion transitions provide the greatest signal-to-noise ratio in a particular assay matrix is complicated. Using in vitro synthesized proteins as analytical standards, we report here an empirically driven method for the selection of said peptides in a human plasma assay matrix.
authors: Department of Genome Sciences, University of Washington, Foege Building S-113B, 3720 15th Avenue, NE, 355065, Seattle, WA, 98195-5065, USA.
authors: James G
authors: JG
authors: Bollinger
authors: Department of Genome Sciences, University of Washington, Foege Building S-113B, 3720 15th Avenue, NE, 355065, Seattle, WA, 98195-5065, USA.
authors: Andrew B
authors: AB
authors: Stergachis
authors: Department of Genome Sciences, University of Washington, Foege Building S-113B, 3720 15th Avenue, NE, 355065, Seattle, WA, 98195-5065, USA.
authors: Richard S
authors: RS
authors: Johnson
authors: Department of Genome Sciences, University of Washington, Foege Building S-113B, 3720 15th Avenue, NE, 355065, Seattle, WA, 98195-5065, USA.
authors: Jarrett D
authors: JD
authors: Egertson
authors: Department of Genome Sciences, University of Washington, Foege Building S-113B, 3720 15th Avenue, NE, 355065, Seattle, WA, 98195-5065, USA. maccoss@u.washington.edu.
authors: Michael J
authors: MJ
authors: MacCoss
mesh_terms: D001798:Blood Proteins / Q000032:analysis; D006801:Humans; D010455:Peptides / Q000032:analysis; D040901:Proteomics / Q000379:methods*
chemical_list: D001798:Blood Proteins; D010455:Peptides
keywords: Human plasma proteome; In vitro translation; Proteotypic peptides; Selected reaction monitoring; Targeted proteomics