title: Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment.
abstract: A fast, economical, and reproducible method for nanoparticle synthesis has been developed in our laboratory. The reaction is performed in an aqueous environment and utilizes light emitted by commercially available 1 W light-emitting diodes (λ =420 nm) as the catalyst. This method does not require nanoparticle seeds or toxic chemicals. The irradiation process is carried out for a period of up to 10 minutes, significantly reducing the time required for synthesis as well as environmental impact. By modulating various reaction parameters silver nanoparticles were obtained, which were predominantly either spherical or cubic. The produced nanoparticles demonstrated strong antimicrobial activity toward the examined bacterial strains. Additionally, testing the effect of silver nanoparticles on the human keratinocyte cell line and human peripheral blood mononuclear cells revealed that their cytotoxicity may be limited by modulating the employed concentrations of nanoparticles.
authors: Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
authors: Rafał
authors: R
authors: Banasiuk
authors: Department of Pathophysiology, Medical University of Gdańsk, Gdańsk, Poland.
authors: Joanna E
authors: JE
authors: Frackowiak
authors: Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
authors: Marta
authors: M
authors: Krychowiak
authors: Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
authors: Marta
authors: M
authors: Matuszewska
authors: Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
authors: Anna
authors: A
authors: Kawiak
authors: Faculty of Materials Science and Ceramics, Department of Ceramics and Refractories, AGH-University of Science and Technology, Kraków, Poland.
authors: Magdalena
authors: M
authors: Ziabka
authors: Institute of Chemical and Environment Engineering, West Pomeranian University of Technology, Szczecin, Poland.
authors: Zofia
authors: Z
authors: Lendzion-Bielun
authors: Faculty of Biology, Laboratory of Electron Microscopy, University of Gdańsk, Gdańsk, Poland.
authors: Magdalena
authors: M
authors: Narajczyk
authors: Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
authors: Aleksandra
authors: A
authors: Krolicka
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D064368:Healthy Volunteers; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D008027:Light; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000528:radiation effects; D014867:Water / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D014867:Water; D012834:Silver
keywords: antimicrobial activity; green synthesis; nanocubes; nanospheres