title: Effects of Poloxamer 188 on red blood cell membrane properties in sickle cell anaemia.
abstract: Vaso-occlusive crisis (VOC) is the main acute complication in sickle cell anaemia (SS) and several clinical trials are investigating different drugs to improve the clinical severity of SS patients. A phase III study is currently exploring the profit of Velopoloxamer in SS during VOCs. We analysed, in-vitro, the effect of poloxamer (P188) on red blood cell (RBC) properties by investigating haemorheology, mechanical and adhesion functions using ektacytometry, microfluidics and dynamic adhesion approaches, respectively. We show that poloxamer significantly reduces blood viscosity, RBC aggregation and adhesion to endothelial cells, supporting the beneficial use of this molecule in SS therapy.
authors: University of Pecs Medical School, Pecs, Hungary.
authors: Barbara
authors: B
authors: Sandor
authors: Inserm UMR_S1134, Paris, France.|Institut National de la Transfusion Sanguine, Paris, France.|Laboratoire d'Excellence GR-Ex, Paris, France.
authors: Mickaël
authors: M
authors: Marin
authors: Inserm UMR_S1134, Paris, France.|Institut National de la Transfusion Sanguine, Paris, France.|Laboratoire d'Excellence GR-Ex, Paris, France.
authors: Claudine
authors: C
authors: Lapoumeroulie
authors: University of Pecs Medical School, Pecs, Hungary.
authors: Miklos
authors: M
authors: Rabaï
authors: Inserm UMR_S1134, Paris, France.|Institut National de la Transfusion Sanguine, Paris, France.|Laboratoire d'Excellence GR-Ex, Paris, France.|Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors: Sophie D
authors: SD
authors: Lefevre
authors: CHU de Pointe-à-Pitre, Pointe-à-Pitre, Guadeloupe.
authors: Nathalie
authors: N
authors: Lemonne
authors: Inserm UMR_S1134, Paris, France.|Institut National de la Transfusion Sanguine, Paris, France.|Laboratoire d'Excellence GR-Ex, Paris, France.
authors: Wassim
authors: W
authors: El Nemer
authors: Laboratoire d'Excellence GR-Ex, Paris, France.|Inserm, Université des Antilles et de la Guyane, 97159, Pointe-à-Pitre, Guadeloupe.
authors: Anaïs
authors: A
authors: Mozar
authors: Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan, France.
authors: Olivier
authors: O
authors: Français
authors: Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan, France.
authors: Bruno
authors: B
authors: Le Pioufle
authors: Laboratoire d'Excellence GR-Ex, Paris, France.|Inserm, Université des Antilles et de la Guyane, 97159, Pointe-à-Pitre, Guadeloupe.|Ecole Normale Supérieure de Cachan, CNRS, BIOMIS-SATIE, UMR 8029, Cachan, France.|Laboratoire CRIS EA647, Section "Vascular Biology and Red Blood Cell", Université Claude Bernard Lyon 1, Lyon, France.|Institut Universitaire de France, Paris, France.
authors: Philippe
authors: P
authors: Connes
authors: Inserm UMR_S1134, Paris, France.|Institut National de la Transfusion Sanguine, Paris, France.|Laboratoire d'Excellence GR-Ex, Paris, France.|Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors: Caroline
authors: C
authors: Le Van Kim
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood* / Q000188:drug therapy / Q000473:pathology; D001809:Blood Viscosity / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D020442:Poloxamer / Q000494:pharmacology*
chemical_list: D020442:Poloxamer
keywords: poloxamer; red blood cell; sickle cell anaemia