title: Potential Therapeutic Applications of Mucuna pruriens Peptide Fractions Purified by High-Performance Liquid Chromatography as Angiotensin-Converting Enzyme Inhibitors, Antioxidants, Antithrombotic and Hypocholesterolemic Agents.
abstract: A Mucuna pruriens protein concentrate was hydrolyzed with a digestive (pepsin-pancreatin) enzymatic system. The soluble portion of the hydrolysate was fractionated by ultrafiltration and the ultrafiltered peptide fraction (PF) with lower molecular weight was purified by reversed-phase high-performance liquid chromatography. The PF obtained were evaluated by testing the biological activity in vitro. Fractions showed that the ability to inhibit the angiotensin-converting enzyme had IC50 values that ranged from 2.7 to 6.2 μg/mL. Trolox equivalent antioxidant capacity values ranged from 132.20 to 507.43 mM/mg. The inhibition of human platelet aggregation ranged from 1.59% to 11.11%, and the inhibition of cholesterol micellar solubility ranged from 0.24% to 0.47%. Hydrophobicity, size, and amino acid sequence could be factors in determining the biological activity of peptides contained in fractions. This is the first report that M. pruriens peptides act as antihypertensives, antioxidants, and inhibitors for human platelet aggregation and cholesterol micellar solubility in vitro.
authors: 1 Faculty of Chemical Engineering, Autonomous University of Yucatan, Merida, Mexico .
authors: Francisco
authors: F
authors: Herrera-Chalé
authors: 2 Department of Chemical-Biochemical Engineering, Institute of Technology of Merida, Merida, Yucatan, Mexico .
authors: Jorge Carlos
authors: JC
authors: Ruiz-Ruiz
authors: 1 Faculty of Chemical Engineering, Autonomous University of Yucatan, Merida, Mexico .
authors: David
authors: D
authors: Betancur-Ancona
authors: 1 Faculty of Chemical Engineering, Autonomous University of Yucatan, Merida, Mexico .
authors: Maira Rubi
authors: MR
authors: Segura-Campos
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D008970:Molecular Weight; D031299:Mucuna / Q000737:chemistry*; D007703:Peptidyl-Dipeptidase A / Q000097:blood; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D005343:Fibrinolytic Agents; D010940:Plant Proteins; D011492:Protein Hydrolysates; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A
keywords: Mucuna pruriens; biological activity; peptide fractions; protein hydrolysate; purification