title: Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging.
abstract: In this paper, the synthesis of alloyed CuInZnxS2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS2 core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells.
authors: Laboratoire Réactions et Génie de Procédés (LRGP), Université de Lorraine, CNRS, UMR 7274, 1 rue Grandville, BP 20451, 54001 Nancy Cedex, France; NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.
authors: Martyna
authors: M
authors: Michalska
authors: NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan 61-866, Poland.
authors: Anna
authors: A
authors: Florczak
authors: Chair of Medical Biotechnology, Poznan University of Medical Sciences, Poznan 61-866, Poland; Department of Diagnostics and Cancer Immunology, Greater Poland Cancer Centre, Poznan 61-866, Poland.
authors: Hanna
authors: H
authors: Dams-Kozlowska
authors: NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Molecular Biophysics Division, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.
authors: Jacek
authors: J
authors: Gapinski
authors: NanoBioMedical Centre, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland; Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.
authors: Stefan
authors: S
authors: Jurga
authors: Laboratoire Réactions et Génie de Procédés (LRGP), Université de Lorraine, CNRS, UMR 7274, 1 rue Grandville, BP 20451, 54001 Nancy Cedex, France. Electronic address: raphael.schneider@univ-lorraine.fr.
authors: Raphaël
authors: R
authors: Schneider
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D016923:Cell Death; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D008299:Maleic Anhydrides / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D013440:Sulfides
chemical_list: D008299:Maleic Anhydrides; D010455:Peptides; D011108:Polymers; D018719:Erb-b2 Receptor Tyrosine Kinases; D013440:Sulfides; C016683:polymaleic anhydride
keywords: Breast cancer; Cancer diagnosis; CuInZn(x)S(2+x) quantum dots; Fluorescence imaging; HER2 targeting; LTVSPWY peptide