title: Performance of an Human Papillomavirus Test in Samples From Women With Histolopathologically Confirmed Invasive Cervical Cancer.
abstract: BACKGROUND
  High-risk human papillomavirus (hrHPV) is now recognized as a single, necessary cause of cancer of the cervix. Although Pap tests have been central to cervical cancer screening programs for more than 50 years, tests that detect infection with these hrHPV genotypes are now being used increasingly in cervical cancer screening programs.
  
  
  OBJECTIVE
  The aim of the study was to determine the sensitivity of an HPV test to detect cervical cancer.
  
  
  METHODS
  Fifty successive cervical samples from women diagnosed with invasive cervical cancer were tested using a molecular HPV DNA test. Thereafter, the residual sample was further tested with a different HPV genotyping test (capable of detecting 27 low- and high-risk types of HPV) and a nucleic acid hybridization test (capable of detecting 13 high-risk types of HPV).
  
  
  RESULTS
  Of the 50 women tested, the first HPV test was positive in 47 cases. Of the 3 negative cases, all were negative by second polymerase chain reaction-based test and 2 were negative by the nucleic acid hybridization test. Human immunodeficiency virus status was positive in 14 women, the majority of whom were positive for HPV 16 (n = 8) and 1 was HPV negative. Most women were diagnosed with having stage II cervical cancer or higher.
  
  
  CONCLUSIONS
  With a sensitivity of 94% of the first HPV test, 6 of 100 cervical cancers will be missed if this was the only test used in a population screening program. The missed cancers were however all clinically detectable.
authors: Departments of 1Obstetrics and Gynaecology and 2Radiation Oncology, University of Cape Town, Cape Town, South Africa; 3Roche Molecular Systems, Pleasanton, CA; 4Department of Pathology and Cell Biology, Columbia University, New York, NY; and 5Department of Pathology, University of Virginia Health System, Charlottesville, VA.
authors: Nomonde
authors: N
authors: Mbatani
authors: Tracey
authors: T
authors: Adams
authors: Leon van
authors: Lv
authors: Wijk
authors: Catherine
authors: C
authors: Behrens
authors: Thanh
authors: T
authors: Tam
authors: Thomas
authors: T
authors: Wright
authors: Mark
authors: M
authors: Stoler
authors: Lynette
authors: L
authors: Denny
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D060005:Genotyping Techniques / Q000379:methods; D061809:Human Papillomavirus DNA Tests / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis*