title: The effect of copper nanoparticles supplementation on freshwater prawn Macrobrachium rosenbergii post larvae.
abstract: The present study was performed to assess the effects of dietary supplementation of copper nanoparticles (Cu-NPs) on growth, biochemical constituents, digestive enzyme activities, antioxidant, metabolic enzyme levels, and non specific immune response of the freshwater prawn, Macrobrachium rosenbergii post larvae (PL). The Cu-NPs (200 nm) were synthesized by facile and environmental friendly hydrothermal method. Cu-NPs were supplemented at 0, 10, 20, 40, 60, and 80 mg kg(-1) with the basal diets. These Cu-NPs supplemented diets were fed to M. rosenbergii PL for 90 days. Results showed significant (P<0.05) improvements were observed in survival, growth, digestive enzyme activities, concentrations of biochemical constituents and total and differential haemocytes count of prawns fed with 20 mg Cu-NPs kg(-1) supplemented feed. Prawns fed with 40-80 mg Cu-NPs kg(-1) supplemented feed showed negative performance. Activity of antioxidants and metabolic enzymes in the muscle and hepatopancreas of prawns showed no significant alterations (P>0.05) prawns fed with up to 20 mg Cu-NPs kg(-1) supplemented feeds. Whereas, prawns fed with 40-80 mg Cu-NPs kg(-1) supplemented feed showed significant (P<0.05) elevations in antioxidant and metabolic enzymes activities. Hence, 40-80 mg Cu-NPs kg(-1) diets may have toxic effect to M. rosenbergii. Hence, present study suggests that 20 mg Cu-NPs kg(-1) can be supplemented for regulating better survival, growth and immune response of M. rosenbergii PL.
authors: Crustacean Biology Laboratory, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. Electronic address: tsmuralisankar@gmail.com.
authors: Thirunavukkarasu
authors: T
authors: Muralisankar
authors: Crustacean Biology Laboratory, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors: Periyakali
authors: P
authors: Saravana Bhavan
authors: Crustacean Biology Laboratory, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors: Subramanian
authors: S
authors: Radhakrishnan
authors: Crustacean Biology Laboratory, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors: Chandirasekar
authors: C
authors: Seenivasan
authors: Crustacean Biology Laboratory, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors: Veeran
authors: V
authors: Srinivasan
mesh_terms: D000818:Animals; D003300:Copper / Q000494:pharmacology*; D005618:Fresh Water; D007814:Larva / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry*; D033541:Palaemonidae / Q000187:drug effects*
chemical_list: D003300:Copper
keywords: Antioxidants; Biochemical constituents; Cu-NPs; Growth; Immune responses; Macrobrachium rosenbergii; Metabolic enzymes