title: A novel neutral, halophile Stachybotrys microspora-based endoglucanase active impact on β-glucan.
abstract: The production of cellulases from Stachybotrys microspora strain (A19) has been improved by fed-batch fermentation on Avicel cellulose 10 mg/ml. An endoglucanase EG2 was purified to homogeneity. This cellulase has a molecular mass estimated to 50 kDa when analyzed by a denaturant gel electrophoresis. It exhibited an optimal activity at 50 °C, pH 7.0 and 0.85 M NaCl. Specifically, these results show the thermo-active, alkali-tolerant and halo-tolerant properties of EG2. In addition, this endoglucanase showed its highest activity on barley-β-glucan, compared to the CMC. Moreover, it was less active on Avicel cellulose. Furthermore, the EG2 activity was stimulated in the presence of EDTA, urea and β-mercaptoethanol whereas it was reduced in the presence of SDS. This cellulase was highly stable in the presence of organic solvents such as acetone and n-hexane. TLC showed that the main hydrolysis products from EG2 were cellobiose and glucose. This fungal endoglucanase could be potentially important in the conversion of grass-derived biomass into fermentable sugars.
authors: Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P 1177, 3018, Sfax, Tunisia.
authors: Ines
authors: I
authors: Benhmad
authors: Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P 1177, 3018, Sfax, Tunisia.
authors: Manel
authors: M
authors: Boudabbous
authors: Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P 1177, 3018, Sfax, Tunisia.
authors: Asma
authors: A
authors: Yaîch
authors: Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P 1177, 3018, Sfax, Tunisia.
authors: Maryem
authors: M
authors: Rebai
authors: Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P 1177, 3018, Sfax, Tunisia. faouzi.gargouri@cbs.rnrt.tn.
authors: Ali
authors: A
authors: Gargouri
mesh_terms: D000096:Acetone / Q000737:chemistry*; D002480:Cellulase / Q000737:chemistry*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry*; D006586:Hexanes / Q000737:chemistry*; D013191:Stachybotrys / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D006586:Hexanes; D000096:Acetone; C026385:n-hexane; D002480:Cellulase
keywords: Barley-β-glucan; Halophile; Neutral endoglucanase; Organic solvents; Stachybotrys microspora