title: Hamadaea flava sp. nov., isolated from a soil sample and emended description of the genus Hamadaea.
abstract: A Gram-stain-positive, aerobic and non-motile actinobacterial strain, designated YIM C0533T, was isolated from a soil sample collected from Shiling county, Yunnan province, south-west China. The isolate grew at 15-37 °C, pH 6.0-8.0 and in the presence of 0-3% (w/v) NaCl. The whole-cell hydrolysates contained meso-diaminopimelic acid, xylose, galactose, mannose, ribose, arabinose, glucose and rhamnose. The acyl type of muramic acid was glycolyl. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The major cellular fatty acids were iso-C16:0, 10-methyl C17:0 and iso-C15 : 0.MK-9(H6) was the predominant menaquinone. The genomic DNA G+C content was determined to be 69.4 mol%. These chemotaxonomic data and the morphological properties were consistent with those of the genus Hamadaea. The strain showed highest sequence similarities to Hamadaea tsunoensis CGMCC 4.1403T on phylogenetic analysis of 16S rRNA gene sequences and was found to form a coherent cluster in the neighbour-joining tree. The DNA-DNA hybridization experiment indicated that the DNA-DNA relatedness value between strain YIM C0533T and H. tsunoensis CGMCC 4.1403T was 34.4±1.3 %. In addition, the results of physiological and biochemical tests allowed the isolate to be differentiated phenotypically from H. tsunoensis CGMCC 4.1403T. On the basis of data from this polyphasic study, strain YIM C0533T is characterized as a novel species of the genus Hamadaea, for which the name Hamadaea flava sp. nov. is proposed. The type strain is YIM C0533T (=CPCC 204160T=KCTC 39591T=CGMCC 4.7289T). An emended description of the genus Hamadaea is also provided.
authors: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PRChina.
authors: Xiao
authors: X
authors: Chu
authors: College of Fisheries, Henan Normal University, Xinxiang, 453007, PRChina.
authors: Shuai
authors: S
authors: Li
authors: China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, PRChina.
authors: Wei
authors: W
authors: Chen
authors: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PRChina.
authors: Mipeshwaree
authors: M
authors: Devi Asem
authors: China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, PRChina.
authors: Yan-Qing
authors: YQ
authors: Duan
authors: College of Fisheries, Henan Normal University, Xinxiang, 453007, PRChina.
authors: Guo-Xing
authors: GX
authors: Nie
authors: Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia.|Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt.
authors: Wael N
authors: WN
authors: Hozzein
authors: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PRChina.
authors: Xiao-Yang
authors: XY
authors: Zhi
authors: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PRChina.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PRChina.
authors: Wen-Jun
authors: WJ
authors: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid