title: φXANES: In vivo imaging of metal-protein coordination environments.
abstract: We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.
authors: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, 3052, Australia.|Australian Synchrotron, Clayton, Victoria, 3168, Australia.
authors: Simon A
authors: SA
authors: James
authors: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, 3052, Australia.|Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales, 2007, Australia.
authors: Dominic J
authors: DJ
authors: Hare
authors: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, 3052, Australia.
authors: Nicole L
authors: NL
authors: Jenkins
authors: Australian Synchrotron, Clayton, Victoria, 3168, Australia.
authors: Martin D
authors: MD
authors: de Jonge
authors: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, 3052, Australia.
authors: Ashley I
authors: AI
authors: Bush
authors: The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, 3052, Australia.
authors: Gawain
authors: G
authors: McColl
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D056831:Coordination Complexes / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007501:Iron / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry*; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D056928:X-Ray Absorption Spectroscopy / Q000379:methods*
chemical_list: D056831:Coordination Complexes; D008667:Metalloproteins; D009942:Organometallic Compounds; D007501:Iron