title: Large-scale biofilm cultivation of Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 for physiologic studies and drug discovery.
abstract: Microbial biofilms are mainly studied due to detrimental effects on human health but they are also well established in industrial biotechnology for the production of chemicals. Moreover, biofilm can be considered as a source of novel drugs since the conditions prevailing within biofilm can allow the production of specific metabolites. Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 when grown in biofilm condition produces an anti-biofilm molecule able to inhibit the biofilm of the opportunistic pathogen Staphylococcus epidermidis. In this paper we set up a P. haloplanktis TAC125 biofilm cultivation methodology in automatic bioreactor. The biofilm cultivation was designated to obtain two goals: (1) the scale up of cell-free supernatant production in an amount necessary for the anti-biofilm molecule/s purification; (2) the recovery of P. haloplanktis TAC125 cells grown in biofilm for physiological studies. We set up a fluidized-bed reactor fermentation in which floating polystyrene supports were homogeneously mixed, exposing an optimal air-liquid interface to let bacterium biofilm formation. The proposed methodology allowed a large-scale production of anti-biofilm molecule and paved the way to study differences between P. haloplanktis TAC125 cells grown in biofilm and in planktonic conditions. In particular, the modifications occurring in the lipopolysaccharide of cells grown in biofilm were investigated.
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126, Naples, Italy. erparril@unina.it.
authors: Ermenegilda
authors: E
authors: Parrilli
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126, Naples, Italy.
authors: Annarita
authors: A
authors: Ricciardelli
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126, Naples, Italy.
authors: Angela
authors: A
authors: Casillo
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126, Naples, Italy.
authors: Filomena
authors: F
authors: Sannino
authors: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185, Rome, Italy.
authors: Rosanna
authors: R
authors: Papa
authors: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185, Rome, Italy.
authors: Marco
authors: M
authors: Tilotta
authors: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185, Rome, Italy.
authors: Marco
authors: M
authors: Artini
authors: Department of Public Health and Infectious Diseases, Sapienza University, Piazzale Aldo Moro 5, 00185, Rome, Italy.
authors: Laura
authors: L
authors: Selan
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126, Naples, Italy.
authors: Maria Michela
authors: MM
authors: Corsaro
authors: Department of Chemical Sciences, Federico II University, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126, Naples, Italy.
authors: Maria Luisa
authors: ML
authors: Tutino
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D019149:Bioreactors; D055808:Drug Discovery / Q000295:instrumentation / Q000379:methods*; D005285:Fermentation; D031161:Pseudoalteromonas / Q000254:growth & development / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: Anti-biofilm; Bacterial biofilm phenotype; Biofilm cultivation; Pseudoalteromonas haloplanktis TAC125