title: Clostridium butyricum in combination with specific immunotherapy converts antigen-specific B cells to regulatory B cells in asthmatic patients.
abstract: The effect of antigen specific immunotherapy (SIT) on asthma is supposed to be improved. Published data indicate that administration of probiotics alleviates allergic diseases. B cells play important roles in the pathogenesis of allergic diseases. This study aims to modulate antigen specific B cell property by the administration of Clostridium butyrate (CB) in combination with SIT. The results showed that after a 3-month treatment, the total asthma clinical score and serum specific IgE were improved in the patients treated with SIT, which was further improved in those treated with both SIT and CB, but not in those treated with CB alone. Treatment with SIT and CB increased p300 and STAT3 activation, up regulated the IL-10 gene transcription and increased the frequency of peripheral antigen specific B cells. In conclusion, administration with SIT in combination with CB converts Der p 1 specific B cells to regulatory B cells in asthma patients allergic to Der p 1. The data suggest a potential therapeutic remedy in the treatment of allergic diseases.
authors: Department of Thoracic Surgery, Caner Center of Guangzhou Medical University, Guangzhou, 510092, China.
authors: Hong-Ying
authors: HY
authors: Liao
authors: Department of Neonate Laboratory, Guangzhou Woman &Children's Medical Center, Guangzhou, 510623, China.
authors: Li
authors: L
authors: Tao
authors: Department of Thoracic Surgery, Caner Center of Guangzhou Medical University, Guangzhou, 510092, China.
authors: Jian
authors: J
authors: Zhao
authors: Department of Radiology, Third Affiliated Hospital, SUN Yat-sen University, Guangzhou 510630, China.
authors: Jie
authors: J
authors: Qin
authors: Department of Microbiology, Zhongshan School of Medicine, Key Laboratory for Tropical Diseases Control of the Ministry of Education, Sun Yat-sen University, Guangzhou, 510080, China.
authors: Gu-Cheng
authors: GC
authors: Zeng
authors: Department of Thoracic Surgery, Caner Center of Guangzhou Medical University, Guangzhou, 510092, China.
authors: Song-Wang
authors: SW
authors: Cai
authors: Department of Thoracic Surgery, Caner Center of Guangzhou Medical University, Guangzhou, 510092, China.
authors: Yun
authors: Y
authors: Li
authors: Department of Thoracic Surgery, Caner Center of Guangzhou Medical University, Guangzhou, 510092, China.
authors: Jian
authors: J
authors: Zhang
authors: Department of Thoracic Surgery, Caner Center of Guangzhou Medical University, Guangzhou, 510092, China.
authors: Hui-Guo
authors: HG
authors: Chen
mesh_terms: D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins / Q000276:immunology*; D001249:Asthma / Q000097:blood / Q000276:immunology / Q000628:therapy; D060151:B-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism; D046972:Clostridium butyricum; D003546:Cysteine Endopeptidases / Q000276:immunology*; D050881:E1A-Associated p300 Protein / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology; D008297:Male; D050796:STAT3 Transcription Factor / Q000097:blood / Q000276:immunology
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C508609:IL10 protein, human; D050796:STAT3 Transcription Factor; D016753:Interleukin-10; D007073:Immunoglobulin E; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1