title: The characterization of hematopoietic tissue in adult Chinese mitten crab Eriocheir sinensis.
abstract: Invertebrates rely on the efficient innate immune mechanisms against invaders, in which the continuous production of hemocytes (hematopoiesis) is indispensable. In the present study, the hematopoietic tissue (HPT) from Chinese mitten crab Eriocheir sinensis was identified and characterized. It was a thin and non-transparent sheet located at the dorsolateral side of the stomach, which was composed of a series of ovoid lobules. Each lobule was surrounded by connective tissue containing a large amount of spherical cells with big nucleus. In HPT, the cells were full of mitochondria and granules, and DNA replication was detected in some cells by EdU labeling technique. Cell proliferation was observed in HPT by transmission electron microscope (TEM). The distribution of two transcription factors, GATA1 and RUNX1, were examined by human GATA1 and RUNX1 antibodies, respectively. Three homologues of RUNX1 were detected in the HPT while no signal of RUNX1 was observed in hemocytes, and GATA1 was detected in both HPT and some hemocytes. The mRNA transcript of a novel hematopoiesis related cytokine EsAst was detected in hepatopancreas and hemocytes, but it was no detectable in HPT. The mRNA expression level of EsAst in hepatopancreas was 1.38-fold higher than that in hemocytes. Total hemocytes counts were related to the mRNA expression level of EsAst post Aeromonas hydrophila challenge. The results suggested that the stem cells in the hematopoietic tissue of Chinese mitten crab E. sinensis were regulated by transcriptional and humoral factors to generate hemocytes.
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Zhihao
authors: Z
authors: Jia
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Sharath
authors: S
authors: Kavungal
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors: Shuai
authors: S
authors: Jiang
authors: Dalian Polytechnic University, Dalian 116034, China.
authors: Depeng
authors: D
authors: Zhao
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors: Mingzhe
authors: M
authors: Sun
authors: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors: Lingling
authors: L
authors: Wang
authors: Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China. Electronic address: lshsong@dlou.edu.cn.
authors: Linsheng
authors: L
authors: Song
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000818:Animals; D060829:Arthropod Proteins / Q000378:metabolism; D003386:Brachyura / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D002452:Cell Count; D050676:Core Binding Factor Alpha 2 Subunit / Q000378:metabolism; D004261:DNA Replication; D050982:GATA1 Transcription Factor / Q000378:metabolism; D015870:Gene Expression; D006410:Hematopoiesis; D006434:Hemocytes / Q000502:physiology / Q000648:ultrastructure; D043143:Hepatopancreas / Q000166:cytology / Q000378:metabolism; D007113:Immunity, Innate
chemical_list: D060829:Arthropod Proteins; D050676:Core Binding Factor Alpha 2 Subunit; D050982:GATA1 Transcription Factor
keywords: EdU; Eriocheir sinensis; Hematopoietic tissue; Histology; Immunohistochemistry; Morphology