title: Permissive growth of human adenovirus type 4 vaccine strain-based vector in porcine cell lines.
abstract: In recent years, there has been considerable interest in using adenoviruses as live vectors to develop recombinant vaccines. Previous studies have demonstrated the safety and effectiveness of HIV/SIV and influenza vaccine candidates based on human adenovirus type 4 (Ad4) replication-competent vectors in rhesus macaque and human model. To explore the possibility of human Ad4 vaccine strain used as a vector in developing porcine vaccines, the growth properties of replication-competent human Ad4 vaccine strain recombinant encoding EGFP in different porcine cell lines were investigated. All tested cell lines are permissive for Ad4 vaccine strain vector with varied replication efficiency. Thus, human Ad4 based vectors would be promising supplement to adenovirus vectors as a delivery vehicle for recombinant vaccines in swine industry.
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Dong-sheng
authors: DS
authors: Gao
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Xiao-jing
authors: XJ
authors: Li
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Wen-yan
authors: WY
authors: Wan
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Hong-jie
authors: HJ
authors: Li
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Xiao-xue
authors: XX
authors: Wang
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Xia
authors: X
authors: Yang
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Yong-tao
authors: YT
authors: Li
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Hong-tao
authors: HT
authors: Chang
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Lu
authors: L
authors: Chen
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China.
authors: Chuan-qing
authors: CQ
authors: Wang
authors: Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Rd., Zhengzhou, Henan 450002, China. Electronic address: zhaoj@henau.edu.cn.
authors: Jun
authors: J
authors: Zhao
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D062705:Adenovirus Vaccines / Q000276:immunology*; D000260:Adenoviruses, Human / Q000254:growth & development* / Q000276:immunology*; D000818:Animals; D002460:Cell Line; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D062705:Adenovirus Vaccines; D014614:Vaccines, Synthetic
keywords: Adenovirus type 4; Live vector; Porcine cell lines; Recombinant vaccine; Replication