title: Cytotoxic activity and chemical constituents of Anthemis mirheydari.
abstract: Context The genus Anthemis L. (Asteraceae) comprises about 195 species which are widely used in the pharmaceutical, cosmetic and food industries. Objective Anthemis mirheydari Iranshar, an endemic plant from Iran, was investigated for its cytotoxic properties and chemical constituents. Materials and methods The whole parts of the plant (320 g) were extracted by dichloromethane and methanol for four days, successively. The cytotoxic activity of both dichloromethane and methanol extracts were assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric methods against three human cancer cell lines including LS180, MCF-7 and MOLT-4. Different concentrations (10-100 μg/mL) of the plant extracts were tested to obtain IC50 values. The dichloromethane extract of A. mirheydari was subjected to silica gel-column and thin layer chromatography for purification of its chemical constituents and the isolated compounds were further tested against MOLT-4 cells. The structures of the pure compounds were elucidated using different spectral data including nuclear magnetic resonance and electron impact mass spectra. Results The IC50 values of the dichloromethane extract were 30.8 ± 6.7, 25.2 ± 6.5 and 8.6 ± 1.1 μg/mL (means ± standard error) for the above-mentioned cell lines, respectively. Two triterpenoids, taraxasterol (1) and pseudotaraxasterol (2), one sterol, β-sitosterol (3) and one coumarin, 7-methoxycoumarin (4) were isolated from the extract. The IC50 of the mixture of compounds 1 and 2 as well as compounds 3 and 4 were higher (>100 μM) than that reported for the dichloromethane extract against MOLT-4 cells. Conclusion The dichloromethane extract was the most active one among the tested material.
authors: a Medicinal and Natural Products Chemistry Research Center , Shiraz University of Medical Sciences , Shiraz , Iran ;
authors: Amir Reza
authors: AR
authors: Jassbi
authors: a Medicinal and Natural Products Chemistry Research Center , Shiraz University of Medical Sciences , Shiraz , Iran ;
authors: Omidreza
authors: O
authors: Firuzi
authors: a Medicinal and Natural Products Chemistry Research Center , Shiraz University of Medical Sciences , Shiraz , Iran ;
authors: Ramin
authors: R
authors: Miri
authors: a Medicinal and Natural Products Chemistry Research Center , Shiraz University of Medical Sciences , Shiraz , Iran ;|b Students Research Committee , Shiraz University of Medical Sciences , Shiraz , Iran ;
authors: Sajad
authors: S
authors: Salhei
authors: a Medicinal and Natural Products Chemistry Research Center , Shiraz University of Medical Sciences , Shiraz , Iran ;|b Students Research Committee , Shiraz University of Medical Sciences , Shiraz , Iran ;
authors: Somayeh
authors: S
authors: Zare
authors: a Medicinal and Natural Products Chemistry Research Center , Shiraz University of Medical Sciences , Shiraz , Iran ;
authors: Mehdi
authors: M
authors: Zare
authors: a Medicinal and Natural Products Chemistry Research Center , Shiraz University of Medical Sciences , Shiraz , Iran ;
authors: Mehdi
authors: M
authors: Masroorbabanari
authors: c Department of Biosynthesis/NMR , Max Planck Institute for Chemical Ecology , Jena , Germany ;
authors: Jima N
authors: JN
authors: Chandran
authors: c Department of Biosynthesis/NMR , Max Planck Institute for Chemical Ecology , Jena , Germany ;
authors: Bernd
authors: B
authors: Schneider
authors: d Department of Molecular Ecology , Max Planck Institute for Chemical Ecology , Jena , Germany.
authors: Ian T
authors: IT
authors: Baldwin
mesh_terms: D031166:Anthemis / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002855:Chromatography, Thin Layer; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D008752:Methylene Chloride / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012997:Solvents; D008752:Methylene Chloride; D002945:Cisplatin; D000432:Methanol
keywords: Asteraceae; cancer; coumarin; triterpenoids