title: Development of Silicalite/Glucose Oxidase-Based Biosensor and Its Application for Glucose Determination in Juices and Nectars.
abstract: The application of silicalite for improvement of enzyme adsorption on new stainless steel electrodes is reported. Glucose oxidase (GOx) was immobilized by two methods: cross-linking by glutaraldehyde (GOx-GA) and cross-linking by glutaraldehyde along with GOx adsorption on silicalite-modified electrode (SME) (GOx-SME-GA). The GOx-SME-GA biosensors were characterized by a four- to fivefold higher sensitivity than GOx-GA biosensor. It was concluded that silicalite together with GA sufficiently enhances enzyme adhesion on stainless steel electrodes. The developed GOx-SME-GA biosensors were characterized by good reproducibility of biosensor preparation (relative standard deviation (RSD)-18 %), improved signal reproducibility (RSD of glucose determination was 7 %), and good storage stability (29 % loss of activity after 18-day storage). A series of fruit juices and nectars was analyzed using GOx-SME-GA biosensor for determination of glucose concentration. The obtained results showed good correlation with the data of high-performance liquid chromatography (HPLC) (R = 0.99).
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143, Kyiv, Ukraine. dc182@yandex.ru.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601, Kyiv, Ukraine. dc182@yandex.ru.
authors: Oleksandr Ye
authors: OY
authors: Dudchenko
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143, Kyiv, Ukraine. victoriya.p@gmail.com.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601, Kyiv, Ukraine. victoriya.p@gmail.com.
authors: Viktoriya M
authors: VM
authors: Pyeshkova
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143, Kyiv, Ukraine. alex_sold@yahoo.com.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601, Kyiv, Ukraine. alex_sold@yahoo.com.
authors: Oleksandr O
authors: OO
authors: Soldatkin
authors: Micro and Nanotechnology Department, Central Laboratory, Middle East Technical University, 06530, Ankara, Turkey. akata@metu.edu.tr.
authors: Burcu
authors: B
authors: Akata
authors: Micro and Nanotechnology Department, Central Laboratory, Middle East Technical University, 06530, Ankara, Turkey. bernaozansoy@gmail.com.
authors: Berna O
authors: BO
authors: Kasap
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143, Kyiv, Ukraine. a_soldatkin@yahoo.com.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601, Kyiv, Ukraine. a_soldatkin@yahoo.com.
authors: Alexey P
authors: AP
authors: Soldatkin
authors: Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143, Kyiv, Ukraine. dzyad@yahoo.com.|Institute of High Technologies, Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01601, Kyiv, Ukraine. dzyad@yahoo.com.
authors: Sergei V
authors: SV
authors: Dzyadevych
keywords: Biosensor; Conductometric transducer; Enzyme immobilization; Glucose oxidase; Silicalite