title: High resolution Physio-chemical Tissue Analysis: Towards Non-invasive In Vivo Biopsy.
abstract: Conventional gold standard histopathologic diagnosis requires information of both high resolution structural and chemical changes in tissue. Providing optical information at ultrasonic resolution, photoacoustic (PA) technique could provide highly sensitive and highly accurate tissue characterization noninvasively in the authentic in vivo environment, offering a replacement for histopathology. A two-dimensional (2D) physio-chemical spectrogram (PCS) combining micrometer to centimeter morphology and chemical composition simultaneously can be generated for each biological sample with PA measurements at multiple optical wavelengths. This spectrogram presents a unique 2D "physio-chemical signature" for any specific type of tissue. Comprehensive analysis of PCS, termed PA physio-chemical analysis (PAPCA), can lead to very rich diagnostic information, including the contents of all relevant molecular and chemical components along with their corresponding histological microfeatures, comparable to those accessible by conventional histology. PAPCA could contribute to the diagnosis of many diseases involving diffusive patterns such as fatty liver.
authors: Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
authors: Guan
authors: G
authors: Xu
authors: Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
authors: Zhuo-Xian
authors: ZX
authors: Meng
authors: Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
authors: Jian-Die
authors: JD
authors: Lin
authors: Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors: Cheri X
authors: CX
authors: Deng
authors: Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
authors: Paul L
authors: PL
authors: Carson
authors: Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
authors: J Brian
authors: JB
authors: Fowlkes
authors: Key Laboratory of Modern Acoustics, Nanjing University, Nanjing, 210093, China.
authors: Chao
authors: C
authors: Tao
authors: Key Laboratory of Modern Acoustics, Nanjing University, Nanjing, 210093, China.
authors: Xiaojun
authors: X
authors: Liu
authors: Department of Radiology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.|Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors: Xueding
authors: X
authors: Wang
mesh_terms: D000818:Animals; D001706:Biopsy; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D005355:Fibrosis; D051379:Mice; D008810:Mice, Inbred C57BL; D061088:Photoacoustic Techniques; D060388:Support Vector Machine