title: Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates.
abstract: The conversion of acetyl-CoA to malonyl-CoA by acetyl-CoA carboxylase (ACC) is the rate-limiting step in fatty acid biosynthesis. In this study, a gene coding for ACC was isolated and characterized from an oleaginous yeast, Lipomyces starkeyi. Real-time quantitative PCR (qPCR) analysis of L. starkeyi acetyl-CoA carboxylase gene (LsACC1) showed that the expression levels were upregulated with the fast accumulation of lipids. The LsACC1 was co-overexpressed with the glycerol 3-phosphate dehydrogenase gene (GPD1), which regulates lipids biosynthesis by supplying another substrates glycerol 3-phosphate for storage lipid assembly, in the non-oleaginous yeast Saccharomyces cerevisiae. Further, the S. cerevisiae acetyl-CoA carboxylase (ScACC1) was transferred with GPD1 and its function was analyzed in comparison with LsACC1. The results showed that overexpressed LsACC1 and GPD1 resulted in a 63% increase in S. cerevisiae. This study gives new data in understanding of the molecular mechanisms underlying the regulation of fatty acids and lipid biosynthesis in yeasts.
authors: a Key Laboratory of Tropical Plant Resources and Sustainable Use , Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences , Kunming , China.|b School of Life Sciences , University of Science and Technology of China , Hefei , China.
authors: Jiancai
authors: J
authors: Wang
authors: c College of Life Sciences , Anhui Science and Technology University , Fengyang , China.
authors: Ronghua
authors: R
authors: Xu
authors: a Key Laboratory of Tropical Plant Resources and Sustainable Use , Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences , Kunming , China.
authors: Ruling
authors: R
authors: Wang
authors: d Key Laboratory for Economic Plants and Biotechnology , Kunming Institute of Botany, Chinese Academy of Sciences , Kunming , China.
authors: Mohammad Enamul
authors: ME
authors: Haque
authors: a Key Laboratory of Tropical Plant Resources and Sustainable Use , Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences , Kunming , China.|d Key Laboratory for Economic Plants and Biotechnology , Kunming Institute of Botany, Chinese Academy of Sciences , Kunming , China.
authors: Aizhong
authors: A
authors: Liu
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D005227:Fatty Acids / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics* / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D055160:Lipomyces / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D008316:Malonyl Coenzyme A / Q000378:metabolism; D060847:Metabolic Engineering; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D019076:Transgenes
chemical_list: D005227:Fatty Acids; D005656:Fungal Proteins; D005994:Glycerophosphates; D008316:Malonyl Coenzyme A; D000105:Acetyl Coenzyme A; C029620:alpha-glycerophosphoric acid; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D000103:Acetyl-CoA Carboxylase
keywords: Lipomyces starkeyi; Saccharomyces cerevisiae; acetyl-CoA carboxylase; fatty acid biosynthesis; glycerol 3-phosphate dehydrogenase