title: Increased Nicotinamide Phosphoribosyltransferase in Rhabdomyosarcomas and Leiomyosarcomas Compared to Skeletal and Smooth Muscle Tissue.
abstract: Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step in NAD synthesis and is up-regulated in several human malignancies, including breast, colon, prostate, thyroid, gastric, and several hematopoietic malignancies. In some malignancies, such as gastric, thyroid, and prostate carcinomas, higher NAMPT expression correlates with deeper tumor invasion, increased metastatic potential and chemotherapy resistance. We employed tissue microarray immunohistochemistry to examine NAMPT expression in benign skeletal and smooth muscle, leiomyomas, leiomyosarcomas (graded low-, intermediate-, and high-grade), and spindle, embryonal, pleomorphic, and alveolar rhabdomyosarcomas. We found low to intermediate NAMPT expression in benign tissue, leiomyomas, leiomyosarcomas (low- and intermediate-grades), and spindle cell rhabdomyosarcomas. In contrast, high-grade leiomyosarcomas and embryonal, alveolar, and pleomorphic rhabdomyosarcomas showed high NAMPT expression. Herein we show for the first time that NAMPT is overexpressed in certain sarcoma types and the level of NAMPT expression correlates with tumor behavior.
authors: Department of Pathology, LSU Health Shreveport, Shreveport, LA, U.S.A.
authors: Moiz
authors: M
authors: Vora
authors: Feist Weiller Cancer Center, Shreveport, LA, U.S.A.
authors: Junaid
authors: J
authors: Ansari
authors: Department of Oral & Maxillofacial/Head and Neck Surgery, LSU Health Sciences Center, Shreveport, LA, U.S.A.
authors: Rabie M
authors: RM
authors: Shanti
authors: Department of Pathology, LSU Health Shreveport, Shreveport, LA, U.S.A.
authors: Diana
authors: D
authors: Veillon
authors: Department of Pathology, LSU Health Shreveport, Shreveport, LA, U.S.A.
authors: James
authors: J
authors: Cotelingam
authors: Department of Anatomic Pathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, U.S.A.
authors: Domenico
authors: D
authors: Coppola
authors: Department of Pathology, LSU Health Shreveport, Shreveport, LA, U.S.A.
authors: Rodney E
authors: RE
authors: Shackelford
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007890:Leiomyosarcoma / Q000378:metabolism* / Q000473:pathology; D018482:Muscle, Skeletal / Q000378:metabolism*; D009130:Muscle, Smooth / Q000378:metabolism*; D009367:Neoplasm Staging; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D011379:Prognosis; D012208:Rhabdomyosarcoma / Q000378:metabolism* / Q000473:pathology; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D016207:Cytokines; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human
keywords: NAMPT; leiomyoma; leiomyosarcoma; rhabdomyosarcoma; skeletal muscle; smooth muscle