title: Coding region variant 186H/R in Exon 4 of APOBEC3G among individuals of Western India.
abstract: The allelic variations in the AIDS restriction genes have been associated with the acquisition of HIV-1 and its progression. The distribution of antiviral gene variants significantly differs between populations. Therefore, we aimed to evaluate the distribution of variant allele of 186H/R in exon4 of APOBEC3G between HIV infected individuals and healthy controls among western Indian.In the present cross-sectional study, we enrolled a total of 153 HIV-infected patients confirmed and 156 unrelated healthy individuals. Polymorphism for 186H/R in exon4 of APOBEC3G gene was genotyped by PCR-RFLP. With the frequency of 186HR heterozygous genotype of APOBEC3G was found to be 13% in healthy controls and none in HIV infected cases. The frequency of 186HH common genotype of APOBEC3G was observed higher in HIV infected individuals compared with healthy controls (100% vs 91.7%). The variant genotype 186RR in APOBEC3G was not found in both the groups. The frequency of 186R allele of APOBEC3G was found 4.16% in healthy controls and nil in HIV-infected cases. The frequency of 186H allele of APOBEC3G was found to be higher in HIV-infected cases compared with healthy controls (100% vs 95.83%). The frequency of 186R allele in exon4 of APOBEC3G was found to be 4.16% in healthy controls. This observation differs from the previous report published from North India stating the absence of 186R allele of APOBEC3G in the North Indian individuals. The variant 186H/R in exon4 of APOBEC3G was neither associated with risk of acquisition of HIV-1 nor its progression.
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.
authors: Hariom
authors: H
authors: Singh
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.
authors: Shruti
authors: S
authors: Marathe
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.|Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Sumitra
authors: S
authors: Nain
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.
authors: Vijay
authors: V
authors: Nema
authors: Department of Molecular Biology, National AIDS Research Institute, Pune, India.|Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Mansa
authors: M
authors: Angadi
authors: Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Shradha
authors: S
authors: Bapat
authors: Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Jyoti
authors: J
authors: Pawar
authors: Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Manisha
authors: M
authors: Ghate
authors: Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Seema
authors: S
authors: Sahay
authors: Department of Clinical Sciences, National AIDS Research Institute, Pune, India.
authors: Raman R
authors: RR
authors: Gangakhedkar
mesh_terms: D000071480:APOBEC-3G Deaminase; D000293:Adolescent; D000328:Adult; D019943:Amino Acid Substitution; D003430:Cross-Sectional Studies; D003564:Cytidine Deaminase / Q000235:genetics*; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D015658:HIV Infections / Q000235:genetics* / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007194:India; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D055815:Young Adult
chemical_list: D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase
keywords: AIDS restriction factor; APOBEC3G; acquisition of HIV-1; coding region polymorphism; genetic susceptibility