title: Heterozygosity of stromelysin-1 (rs3025058) promoter polymorphism is associated with gastric cancer.
abstract: BACKGROUND
  Gastric cancer (GC) is the third most common cancer in India and is mediated by multiple genetic, epigenetic and environmental risk factors. A single nucleotide polymorphism rs3025058 at -1171 of the stromelysin-1 (matrix metalloproteinase [MMP]-3) promoter is resulting due to insertion/deletion of adenine thought to have an impact on increasing the risk for tumor formation.
  
  
  OBJECTIVE
  This study is aimed to understand the role of stromelysin-1 rs3025058 (-1171, 5A/6A) promoter polymorphism in the etiology of GC in Indian population.
  
  
  METHODS
  Genomic DNA was isolated from blood samples of the GC patients and controls. The genotyping of stromelysin-1 rs3025058 (-1171, 5A/6A) promoter polymorphism was carried out by amplification refractory mutation system-polymerase chain reaction method followed by agarose gel electrophoresis.
  
  
  RESULTS
  The frequency of 5A/5A, 5A/6A, and 6A/6A genotypes in GC patients were 7.69%, 76.92%, and 15.38%, while in controls were 5.31%, 86.73%, and 7.96%, respectively. There was a significant difference in the distribution of 5A/6A genotype in patients compared to the controls (P < 0.05).
  
  
  CONCLUSIONS
  This study showed an increased frequency of heterozygotes for stromelysin-1 rs3025058 and thought to be involved in the etiology of GC.
authors: D
authors: D
authors: Krishnaveni
authors: A C
authors: AC
authors: Bhayal
authors: K P
authors: KP
authors: Shravan
authors: A
authors: A
authors: Jyothy
authors: N
authors: N
authors: Pratibha
authors: Department of Cell Biology, Institute of Genetics and Hospital for Genetic Diseases, Hyderabad, India.
authors: A
authors: A
authors: Venkateshwari
mesh_terms: D000328:Adult; D000368:Aged; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D007194:India; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D019278:Matrix Metalloproteinase 3