title: Effects of the Antibiotics Growth Promoter Tylosin on Swine Gut Microbiota.
abstract: Tylosin has been used as a livestock feed additive and antibiotic growth promoter for many years. However, the mode of action by which tylosin enhances animal growth is unclear. We used high-throughput sequencing of 16S rRNA genes to investigate the effects of tylosin as a feed additive on swine gut microbiota. No significant difference in the rate of weight increase was observed between control and tylosin-treated pigs during a 10-week feeding trial. However, tylosin-treated pigs showed rapid increases in the relative abundance of the phylum Firmicutes. Increases in Firmicutes species are associated with (so-called) obese-type gut microbiota. The abundance of species of four families of the phylum Firmicutes (Streptococcaceae, Peptococcaceae, Peptostreptococcaceae, and Clostridiaceae) correlated positively with host weight gain. The abundance of Streptococcaceae family bacteria was least affected by tylosin treatment. Distribution analysis of operational taxonomic units (OTUs) showed that both control and tylosin-treated pigs exhibited similar OTU alterations during growth. However, the tylosin-treated group showed distinctive alterations in gut microbiota when the host weighed approximately 60 kg, whereas similar alterations occurred at around 80 kg in the control group. Our results suggest that use of tylosin accelerates maturation of swine gut microbiota rather than altering its composition.
authors: Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University, Jeju 63243, Republic of Korea.
authors: Jungman
authors: J
authors: Kim
authors: Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University, Jeju 63243, Republic of Korea.
authors: Robin Becina
authors: RB
authors: Guevarra
authors: Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University, Jeju 63243, Republic of Korea.|Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Cau Giay, Hanoi, Vietnam.
authors: Son Giang
authors: SG
authors: Nguyen
authors: Department of Bioenvironmental Chemistry, Chonbuk National University, Jeonju 54896, Republic of Korea.
authors: Ji-Hoon
authors: JH
authors: Lee
authors: Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University, Jeju 63243, Republic of Korea.
authors: Dong Kee
authors: DK
authors: Jeong
authors: Faculty of Biotechnology, College of Applied Life Science, SARI, Jeju National University, Jeju 63243, Republic of Korea.
authors: Tatsuya
authors: T
authors: Unno
mesh_terms: D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification* / Q000187:drug effects*; D004269:DNA, Bacterial / Q000235:genetics; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology; D054892:Metagenome; D023421:Models, Animal; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine / Q000254:growth & development / Q000382:microbiology*; D015645:Tylosin / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D006133:Growth Substances; D012336:RNA, Ribosomal, 16S; D015645:Tylosin
keywords: 16S rRNA gene; antibiotics; growth promoter; swine gut microbiota; tylosin