title: A Live Vector Expressing HPV16 L1 Generates an Adjuvant-Induced Antibody Response In-vivo.
abstract: BACKGROUND
  The association between human papillomavirus (HPV) infections and cervical cancer has suggested the design of prophylactic and therapeutic vaccines against genital warts. The HPV capsid has made of two L1 and L2 coat proteins that have produced late in viral infections. Regarding to the recent studies, two commercial prophylactic vaccines have based on L1 viral like particles (VLPs) could strongly induce antibody responses, and protect human body from HPV infections. However, the use of these HPV vaccines has hindered due to their high cost and some limitations. Currently, among various vaccination strategies, live vector-based vaccines have attracted a great attention.
  
  
  OBJECTIVE
  Herein, a non-pathogenic strain of the protozoan organism known as Leishmania tarentolae has utilized to induce potent humoral immunity in mice model.
  
  
  METHODS
  At first, cloning of HPV16 L1 gene into Leishmania expression vector has performed and confirmed by PCR and digestion with restriction enzymes. The promastigotes of Leishmania tarentolae (L.tar) have transfected with linearized DNA construct by electroporation. Protein expression has analyzed by SDS-PAGE and western blotting. Then, the immunogenicity of leishmania expressing L1 protein (L.tar-L1) has assessed in mice model.
  
  
  RESULTS
  Our data has indicated that subcutaneous immunization of mice with the recombinant L.tar-L1 has led to enhance the levels of IgG1 and lgG2a in comparison with control groups. Furthermore, there was no significant increase in antibody levels between two and three times of immunizations.
  
  
  CONCLUSIONS
  The recombinant live vector was able to induce humoral immunity in mice without need of any adjuvant. However, further studies have required to increase its efficiency.
authors: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, IR Iran; Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
authors: Zeinab
authors: Z
authors: Shirbaghaee
authors: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, IR Iran.
authors: Azam
authors: A
authors: Bolhassani
authors: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.
authors: Abbas
authors: A
authors: Mirshafiey
authors: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, IR Iran.
authors: Fatemeh
authors: F
authors: Motevalli
authors: Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, IR Iran.
authors: Negar
authors: N
authors: Zohrei
keywords: HPV; L1 Protein; Live Vaccine