title: Differentiation of human gingival mesenchymal stem cells into neuronal lineages in 3D bioconjugated injectable protein hydrogel construct for the management of neuronal disorder.
abstract: The success of regeneration attempt is based on an ideal combination of stem cells, scaffolding and growth factors. Tissue constructs help to maintain stem cells in a required area for a desired time. There is a need for easily obtainable cells, potentially autologous stem cells and a biologically acceptable scaffold for use in humans in different difficult situations. This study aims to address these issues utilizing a unique combination of stem cells from gingiva and a hydrogel scaffold, based on a natural product for regenerative application. Human gingival mesenchymal stem cells (HGMSCs) were, with due induction, differentiated to neuronal lineages to overcome the problems associated with birth tissue-related stem cells. The differentiation potential of neuronal lineages was confirmed with suitable specific markers. The properties of mesenchymal stem cells in encapsulated form were observed to be similar to free cells. The encapsulated cells (3D) were then subjected to differentiation into neuronal lineages with suitable inducers, and the morphology and gene expression of transient cells were analyzed. HGMSCs was differentiated into neuronal lineages as both free and encapsulated forms without any significant differences. The presence of Nissl bodies and the neurite outgrowth confirm the differentiation. The advantages of this new combination appear to make it a promising tissue construct for translational application.
authors: Department of Periodontology and Implantology, Faculty of Dental Sciences, Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra University, Chennai, India.
authors: Suresh Ranga
authors: SR
authors: Rao
authors: Centre for Regenerative Medicine and Stem Cell Research, Central Research Facility, Sri Ramachandra University, Chennai, India.
authors: Rajasekaran
authors: R
authors: Subbarayan
authors: Centres for Indian Systems of Medicine Quality Assurance and Standardization, Sri Ramachandra University, Chennai, India.
authors: Murugan Girija
authors: MG
authors: Dinesh
authors: Microbiology Division, Central Leather Research Institute Adyar, Chennai, India.
authors: Gnanamani
authors: G
authors: Arumugam
authors: Microbiology Division, Central Leather Research Institute Adyar, Chennai, India.
authors: Selvaraj Thirupathi Kumara
authors: ST
authors: Raja
mesh_terms: D000328:Adult; D015415:Biomarkers; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D012038:Regeneration; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D055815:Young Adult
chemical_list: D015415:Biomarkers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate