title: Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury.
abstract: FGF-10 can prevent or reduce lung specific inflammation due to traumatic or infectious lung injury. However, the exact mechanisms are poorly characterized. Additionally, the effect of FGF-10 on lung-resident mesenchymal stem cells (LR-MSCs) has not been studied. To better characterize the effect of FGF-10 on LR-MSCs, FGF-10 was intratracheally delivered into the lungs of rats. Three days after instillation, bronchoalveolar lavage was performed and plastic-adherent cells were cultured, characterized and then delivered therapeutically to rats after LPS intratracheal instillation. Immunophenotyping analysis of FGF-10 mobilized and cultured cells revealed expression of the MSC markers CD29, CD73, CD90, and CD105, and the absence of the hematopoietic lineage markers CD34 and CD45. Multipotency of these cells was demonstrated by their capacity to differentiate into osteocytes, adipocytes, and chondrocytes. Delivery of LR-MSCs into the lungs after LPS injury reduced the inflammatory response as evidenced by decreased wet-to-dry ratio, reduced neutrophil and leukocyte recruitment and decreased inflammatory cytokines compared to control rats. Lastly, direct delivery of FGF-10 in the lungs of rats led to an increase of LR-MSCs in the treated lungs, suggesting that the protective effect of FGF-10 might be mediated, in part, by the mobilization of LR-MSCs in lungs.
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Lin
authors: L
authors: Tong
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Jian
authors: J
authors: Zhou
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Linyi
authors: L
authors: Rong
authors: Division of Pulmonary and Critical Care Medicine, University of California, San Francisco, California 94143, USA.
authors: Eric J
authors: EJ
authors: Seeley
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Jue
authors: J
authors: Pan
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Xiaodan
authors: X
authors: Zhu
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Jie
authors: J
authors: Liu
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Qin
authors: Q
authors: Wang
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
authors: Xinjun
authors: X
authors: Tang
authors: Department of Pulmonary Medicine, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, P. R. China.
authors: Jieming
authors: J
authors: Qu
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.|State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou, 510120, P. R. China.
authors: Chunxue
authors: C
authors: Bai
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.|Shanghai Public Health Clinical Center, Shanghai, 201508, P. R. China.|Zhongshan Hospital, Qingpu branch, Fudan University, Shanghai, 201700, P. R. China.
authors: Yuanlin
authors: Y
authors: Song
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D051526:Fibroblast Growth Factor 10 / Q000276:immunology*; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D016130:Immunophenotyping; D008070:Lipopolysaccharides; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000276:immunology*; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017468:Receptors, Fibroblast Growth Factor / Q000276:immunology
chemical_list: C496465:Fgf10 protein, rat; D051526:Fibroblast Growth Factor 10; D008070:Lipopolysaccharides; D017468:Receptors, Fibroblast Growth Factor