title: High Frequency of Human Papillomavirus Genotype 16 Among Patients With Anogenital Warts.
abstract: BACKGROUND
  Human Papillomavirus (HPV) infection is considered the most prevalent sexually transmitted virus infection. Human Papillomavirus 16 and 18 have been documented as high-risk HPV infections and responsible for 70% of all cervical cancers.
  
  
  OBJECTIVE
  The aim of this study was to determine HPV genotypes in patients with anogenital warts.
  
  
  METHODS
  In this study lesion samples were collected from 54 patients with an age ranged of 19 to 44 years. Initially, DNA extraction was carried out for all samples followed by detection of HPV DNA by the polymerase chain reaction. The positive PCR products were sequenced and the results were blasted to determine HPV genotypes.
  
  
  RESULTS
  Out of 54 samples, 46 (85.18%) cases showed positive results for HPV DNA. A total of 26 (56.6%) samples were males and 20 (43.4%) females while eight (14.81%) showed HPV negative results. Overall, 37 (80%) patients had multiple sexual partners, and nine (20%) had one sexual partner. The frequency of anogenital warts was higher in married patients. The results of sequencing revealed that frequency of HPV16, HPV11 and HPV6 was 58.69%, 26.08% and 15.21%, respectively.
  
  
  CONCLUSIONS
  Human Papillomavirus 16 as a high risk HPV was found to have the highest frequency among patients with anogenital warts.
authors: Department of Dermatology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Reza
authors: R
authors: Yaghoobi
authors: Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Manoochehr
authors: M
authors: Makvandi
authors: Department of Dermatology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Nasim
authors: N
authors: Afshar
authors: Department of Dermatology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Nader
authors: N
authors: Pazyar
authors: Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Mojtaba
authors: M
authors: Hamidifard
authors: Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors: Chia
authors: C
authors: Sharifpour
keywords: Condylomata acuminata; Genotype; Human Papillomavirus; Polymerase Chain Reaction