title: Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein.
abstract: Influenza viruses that express reporter proteins are useful tools, but are often attenuated. Recently, we found that an influenza virus encoding the Venus fluorescent protein acquired two mutations in its PB2 and HA proteins upon mouse adaptation. Here, we demonstrate that the enhanced viral replication and virulence in mice of this Venus-expressing influenza virus are primarily conferred by the PB2-E712D mutation, with only a minor contribution by the HA-T380A mutation.
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Hiroaki
authors: H
authors: Katsura
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.|Exploratory Research for Advanced Technology, Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan.
authors: Satoshi
authors: S
authors: Fukuyama
authors: Exploratory Research for Advanced Technology, Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan.|Laboratory of Veterinary Microbiology, Department of Veterinary Sciences, University of Miyazaki, Miyazaki, Japan.
authors: Shinji
authors: S
authors: Watanabe
authors: Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.|Transboundary Animal Distance Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan.
authors: Makoto
authors: M
authors: Ozawa
authors: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
authors: Gabriele
authors: G
authors: Neumann
authors: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.|Exploratory Research for Advanced Technology, Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan.|Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.|Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors: Yoshihiro
authors: Y
authors: Kawaoka
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D004285:Dogs; D005260:Female; D017930:Genes, Reporter; D057809:HEK293 Cells; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D008561:Membrane Fusion; D051379:Mice; D008810:Mice, Inbred C57BL; D009975:Orthomyxoviridae / Q000254:growth & development / Q000378:metabolism*; D009976:Orthomyxoviridae Infections / Q000401:mortality / Q000473:pathology / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
chemical_list: D006389:Hemagglutinins, Viral; D008164:Luminescent Proteins; C502893:PB2 protein, influenza virus; D014764:Viral Proteins