title: A modified bioautographic method for antibacterial component screening against anaerobic and microaerophilic bacteria.
abstract: Direct bioautography is a useful method to identify antimicrobial compounds with potential therapeutic importance. Because of technical limitations till now, it has been applied only for aerobic bacteria. In this work we present the modification of the original method by which antimicrobial screening of bacteria requiring modified atmosphere became feasible by direct bioautography. Here we demonstrate its applicability by testing three anaerobic Clostridium perfringens and three microaerophilic Campylobacter jejuni strains against two essential oils, clove and thyme. Antimicrobial component profiles of clove and thyme essential oils against these two medically important pathogenic bacteria were compared and significant differences were revealed in their inhibition capacities. Linalool, a component of thyme essential oil exerted a more expressed antibacterial activity against C. perfringens than against C. jejuni. Our results demonstrate that direct bioautography is not only suitable for testing aerobic bacteria, but by applying the presently described modified version it can also contribute to the quest to find novel antimicrobial agents against multidrug resistant anaerobic and microaerophilic bacteria.
authors: Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary.
authors: Judit K
authors: JK
authors: Kovács
authors: Department of Pharmacognosy, Medical School, University of Pécs, Hungary.
authors: Györgyi
authors: G
authors: Horváth
authors: Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary.
authors: Monika
authors: M
authors: Kerényi
authors: Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary.
authors: Béla
authors: B
authors: Kocsis
authors: Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary; Centre for Agricultural Research, Institute for Veterinary Medical Research, Budapest, Hungary.
authors: Levente
authors: L
authors: Emődy
authors: Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary. Electronic address: gyorgy.schneider@aok.pte.hu.
authors: György
authors: G
authors: Schneider
mesh_terms: D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000187:drug effects* / Q000378:metabolism; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009822:Oils, Volatile / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D027842:Syzygium / Q000737:chemistry; D046930:Thymus Plant / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D010938:Plant Oils; D010100:Oxygen
keywords: Antibacterial effect; Campylobacter jejuni; Clostridium perfringens; Direct bioautography; Essential oils