title: The Effect of Small Cosolutes that Mimic Molecular Crowding Conditions on the Stability of Triplexes Involving Duplex DNA.
abstract: Triplex stability is studied in crowding conditions using small cosolutes (ethanol, acetonitrile and dimethylsulfoxide) by ultraviolet (UV), circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopies. The results indicate that the triplex is formed preferentially when the triplex forming oligonucleotide (TFO) is RNA. In addition, DNA triplexes (D:D·D) are clearly less stable in cosolute solutions while the stability of the RNA triplexes (R:D·D) is only slightly decreased. The kinetic of triplex formation with RNA-TFO is slower than with DNA-TFO and the thermal stability of the triplex is increased with the salt concentration in EtOH-water solutions. Accordingly, RNA could be considered a potential molecule to form a stable triplex for regulatory purposes in molecular crowding conditions.
authors: Institute for Advanced Chemistry of Catalonia (IQAC), CSIC, Jordi Girona 18-26, Barcelona 08034, Spain. aaagma@cid.csic.es.|Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Jordi Girona 18-26, Barcelona 08034, Spain. aaagma@cid.csic.es.
authors: Anna
authors: A
authors: Aviñó
authors: Department of Food, Environmental and Nutritional Sciences (DEFENS), Section of Chemical and Biomolecular Sciences, University of Milan, Via Celoria 2, Milan 20133, Italy. stefania.mazzini@unimi.it.
authors: Stefania
authors: S
authors: Mazzini
authors: Department of Analytical Chemistry, University of Barcelona, Martí i Franquès, 1-11, Barcelona 08028, Spain. raimon_gargallo@ub.edu.
authors: Raimundo
authors: R
authors: Gargallo
authors: Institute for Advanced Chemistry of Catalonia (IQAC), CSIC, Jordi Girona 18-26, Barcelona 08034, Spain. recgma@cid.csic.es.|Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Jordi Girona 18-26, Barcelona 08034, Spain. recgma@cid.csic.es.
authors: Ramon
authors: R
authors: Eritja
mesh_terms: D002412:Cations / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D013816:Thermodynamics; D044366:Transition Temperature
chemical_list: D002412:Cations; C051802:triplex DNA; D004247:DNA
keywords: DNA; RNA; cosolute; molecular crowding; thermal stability; triplex forming oligonucleotide (TFO)