title: Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene.
abstract: The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which completely rule out the concern of human pathogen contamination. DAS-NG exhibited advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. hPSC cultured on DAS-NG could maintain pluripotency in vitro and in vivo, and especially cell adhesion-related gene expression profile was comparable to those of cultured on feeders, while hPSC cultured without DAS-NG differentiated spontaneously with high expression of somatic cell-enriched adhesion genes. This feeder-free and xeno-free culture method using DAS-NG will facilitate the generation of clinical-grade hPSC.
authors: Hans Schöler Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.
authors: Hyunah
authors: H
authors: Lee
authors: Hans Schöler Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.
authors: Donggyu
authors: D
authors: Nam
authors: SMEs Support Center, Korea Institute of Science and Technology Information, 48058 Busan, South Korea.
authors: Jae-Kyung
authors: JK
authors: Choi
authors: Group of Computational Biology and Bioinformatics, Biodonostia Health Research Institute, 20014 San Sebastián, Spain.|IKERBASQUE, Basque Foundation for Science, Bilbao, Spain.
authors: Marcos J
authors: MJ
authors: Araúzo-Bravo
authors: Hans Schöler Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.|School of Materials Science and Engineering, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.
authors: Soon-Yong
authors: SY
authors: Kwon
authors: Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Münster, Germany.
authors: Holm
authors: H
authors: Zaehres
authors: Hans Schöler Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.
authors: Taehee
authors: T
authors: Lee
authors: Hans Schöler Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.
authors: Chan Young
authors: CY
authors: Park
authors: Hans Schöler Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.
authors: Hyun-Wook
authors: HW
authors: Kang
authors: Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Münster, Germany.
authors: Hans R
authors: HR
authors: Schöler
authors: Hans Schöler Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea.
authors: Jeong Beom
authors: JB
authors: Kim
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D061252:Feeder Cells / Q000166:cytology; D015870:Gene Expression; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D006108:Graphite