title: Sox2 contributes to tooth development via Wnt signaling.
abstract: The transcription factor Sox2 is a stem cell marker that dictates cell lineage. It has been shown to mark the epithelial stem cells of the continuously growing mouse incisors. Sox2 also interferes with Wnt signaling by binding to β-catenin, a central mediator of the Wnt pathway. We show that these functions of Sox2 are essential for mouse molar development. Sox2 has previously been shown to play a role in the formation of new teeth from the existing dental epithelium. To assess Sox2 function related to cell migration within a tooth, we monitored cell movement by using a DiI system and observed that DiI moves from molar 1 to molar 2 during tooth development. However, upon temporal knockdown of Sox2, DiI remains in the molar 1 region. This study also provides novel insights into the role of Sox2 and the important validation of Sox2 as a potent target in Wnt signaling during tooth development. Our data reveal that the degradation of Wnt signaling caused by the knockdown of Sox2 results in a lack of cell migration during tooth development.
authors: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemoon-Gu, Seoul, 03722, South Korea.
authors: Min-Jung
authors: MJ
authors: Lee
authors: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemoon-Gu, Seoul, 03722, South Korea.
authors: Eun-Jung
authors: EJ
authors: Kim
authors: Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University, Yahaba, Japan.
authors: Keishi
authors: K
authors: Otsu
authors: Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University, Yahaba, Japan.
authors: Hidemitsu
authors: H
authors: Harada
authors: Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemoon-Gu, Seoul, 03722, South Korea. hsjung@yuhs.ac.|Oral Biosciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, People's Republic of China. hsjung@yuhs.ac.
authors: Han-Sung
authors: HS
authors: Jung
mesh_terms: D000818:Animals; D002465:Cell Movement; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D051379:Mice; D034741:RNA, Small Interfering / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D013997:Time Factors; D014070:Tooth / Q000196:embryology* / Q000378:metabolism*; D060449:Wnt Signaling Pathway
chemical_list: D034741:RNA, Small Interfering; D055748:SOXB1 Transcription Factors
keywords: Cell migration; DiI system; Sox2; Tooth; Wnt signaling