title: Loss of Adipose Fatty Acid Oxidation Does Not Potentiate Obesity at Thermoneutrality.
abstract: Ambient temperature affects energy intake and expenditure to maintain homeostasis in a continuously fluctuating environment. Here, mice with an adipose-specific defect in fatty acid oxidation (Cpt2(A-/-)) were subjected to varying temperatures to determine the role of adipose bioenergetics in environmental adaptation and body weight regulation. Microarray analysis of mice acclimatized to thermoneutrality revealed that Cpt2(A-/-) interscapular brown adipose tissue (BAT) failed to induce the expression of thermogenic genes such as Ucp1 and Pgc1α in response to adrenergic stimulation, and increasing ambient temperature exacerbated these defects. Furthermore, thermoneutral housing induced mtDNA stress in Cpt2(A-/-) BAT and ultimately resulted in a loss of interscapular BAT. Although the loss of adipose fatty acid oxidation resulted in clear molecular, cellular, and physiologic deficits in BAT, body weight gain and glucose tolerance were similar in control and Cpt2(A-/-) mice in response to a high-fat diet, even when mice were housed at thermoneutrality.
authors: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Metabolism and Obesity Research, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Jieun
authors: J
authors: Lee
authors: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Metabolism and Obesity Research, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Joseph
authors: J
authors: Choi
authors: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Metabolism and Obesity Research, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Susan
authors: S
authors: Aja
authors: Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors: Susanna
authors: S
authors: Scafidi
authors: Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Metabolism and Obesity Research, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: mwolfga1@jhmi.edu.
authors: Michael J
authors: MJ
authors: Wolfgang
mesh_terms: D042964:Acyl-CoA Dehydrogenase / Q000235:genetics / Q000378:metabolism; D000222:Adaptation, Physiological; D002001:Adipose Tissue, Brown / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D001835:Body Weight; D002334:Carnitine O-Palmitoyltransferase / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D005227:Fatty Acids / Q000378:metabolism*; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000235:genetics; D008661:Metabolism, Inborn Errors / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000201:enzymology / Q000209:etiology / Q000235:genetics / Q000473:pathology; D010084:Oxidation-Reduction; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013696:Temperature; D022722:Thermogenesis / Q000235:genetics*; D000071256:Uncoupling Protein 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D005227:Fatty Acids; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; D042964:Acyl-CoA Dehydrogenase; C558401:CPT1B protein, mouse; D002334:Carnitine O-Palmitoyltransferase