title: The effect of diabetes on the wound healing potential of adipose-tissue derived stem cells.
abstract: To investigate whether diabetes mellitus affects the wound-healing-promoting potential of adipose tissue-derived stem cells, we designed a wound-healing model using diabetic mice. We compared the degree of wound healing between wounds treated with normal adipose tissue-derived stem cells and wounds treated with diabetic adipose tissue-derived stem cells. We evaluated the wound-healing rate, the epithelial tongue distance, the area of granulation tissue, the number of capillary and the number of Ki-67-stained cells. The wound-healing rate was significantly higher in the normal adipose tissue-derived stem cells group than in the diabetic adipose tissue-derived stem cells group; it was also significantly higher in the normal adipose tissue-derived stem cells group than in the control group. Although the diabetic adipose tissue-derived stem cells group showed a better wound-healing rate than the control group, the difference was not statistically significant. Similar trends were observed for the other parameters examined: re-epithelisation and keratinocyte proliferation; granulation tissue formation; and dermal regeneration. However, with regard to the number of capillary, diabetic adipose tissue-derived stem cells retained their ability to promote neovasculisation and angiogenesis. These results reflect the general impairment of the therapeutic potential of diabetic adipose tissue-derived stem cells in vivo.
authors: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Yeouido St. Mary's Hospital, Seoul, Korea.
authors: Sue Min
authors: SM
authors: Kim
authors: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Yeouido St. Mary's Hospital, Seoul, Korea.
authors: Yun Ho
authors: YH
authors: Kim
authors: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Bucheon St. Mary's Hospital, Seoul, Korea.
authors: Young Joon
authors: YJ
authors: Jun
authors: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Yeouido St. Mary's Hospital, Seoul, Korea.
authors: Gyeol
authors: G
authors: Yoo
authors: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul St. Mary's Hospital, Seoul, Korea.
authors: Jong Won
authors: JW
authors: Rhie
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002196:Capillaries / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D006097:Granulation Tissue / Q000473:pathology; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000473:pathology
keywords: adipose tissue; adult stem cell; cell therapy; diabetes mellitus; wound healing