title: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Gene Polymorphisms and Hepatitis B Virus Infection.
abstract: BACKGROUND
  Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an apoptotic molecule with a key role in the apoptosis of tumors and virus-infected cells. The association of 1525G/A and 1595C/T polymorphisms in the region of 3' UTR on the TRAIL gene has been shown in many cancers and diseases. Polymorphism at the positions of 1525G/A and 1595C/T might influence the clearance of hepatitis B virus (HBV).
  
  
  OBJECTIVE
  This study was carried out to determine the role of the TRAIL gene polymorphisms in clinical outcome of HBV infection.
  
  
  METHODS
  Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) was applied to genotype TRAIL polymorphisms at positions 1525G/A and 1595C/T. To evaluate the TRAIL gene polymorphism in the 3' UTR region at position 1525G/A and 1595C/T, 147 patients with HBV infection were divided into three different groups of chronic hepatitis (n = 52), cirrhosis (n = 33), and carrier (n = 62) and there was a group of 101 healthy controls.
  
  
  RESULTS
  Our data showed that genotypes 1525G/A and 1595C/T were in complete linkage disequilibrium and the genotype frequencies at the two positions were the same. No significant differences in frequencies of genotype and alleles at positions 1525G/A and 1595C/T were observed between all the three groups (P value > 0.05).
  
  
  CONCLUSIONS
  According to our result, 1525G/A and 1595C/T were in strong linkage disequilibrium and the polymorphisms of the TRAIL gene in the 3' UTR region were not associated with the outcome of HBV infection.
authors: Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors: Anvar
authors: A
authors: Soleimani
authors: Inflammation and Inflammatory Diseases Research Centre, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors: Houshang
authors: H
authors: Rafatpanah
authors: Department of Immunogenetic and Cell Culture, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors: Amin Reza
authors: AR
authors: Nikpoor
authors: Department of Basic Medical Science, Neyshabur University of Medical Sciences, Neyshabur, IR Iran.
authors: Mehrdad
authors: M
authors: Kargari
authors: Biochemistry and Nutrition Research Center, Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
authors: Daryoush
authors: D
authors: Hamidi Alamdari
keywords: Hepatitis B Virus; Polymorphism; Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand