title: Sequestering survivin to functionalized nanoparticles: a strategy to enhance apoptosis in cancer cells.
abstract: Survivin belongs to the family of inhibitor of apoptosis proteins (IAP) and is present in most cancers while being below detection limits in most terminally differentiated adult tissues, making it an attractive protein to target for diagnostic and, potentially, therapeutic roles. Sub-100 nm poly(propargyl acrylate) (PA) particles were surface modified through the copper-catalyzed azide/alkyne cycloaddition of an azide-terminated survivin ligand derivative (azTM) originally proposed by Abbott Laboratories and speculated to bind directly to survivin (protein) at its dimer interface. Using affinity pull-down studies, it was determined that the PA/azTM nanoparticles selectively bind survivin and the particles can enhance apoptotic cell death in glioblastoma cell lines and other survivin over-expressing cell lines such as A549 and MCF7 relative to cells incubated with the original Abbott-derived small molecule inhibitor.
authors: Center for Optical Materials Science and Engineering Technologies, Department of Materials Science & Engineering, Clemson University, Clemson, SC 29634, USA. foulger@clemson.edu.
authors: Ragini
authors: R
authors: Jenkins
authors: Yuriy P
authors: YP
authors: Bandera
authors: Michael A
authors: MA
authors: Daniele
authors: LeAnna L
authors: LL
authors: Ledford
authors: Ashlee
authors: A
authors: Tietje
authors: Andrew A
authors: AA
authors: Kelso
authors: Michael G
authors: MG
authors: Sehorn
authors: Yanzhang
authors: Y
authors: Wei
authors: Mrinmay
authors: M
authors: Chakrabarti
authors: Swapan K
authors: SK
authors: Ray
authors: Stephen H
authors: SH
authors: Foulger
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D017209:Apoptosis / Q000502:physiology; D001386:Azides / Q000737:chemistry* / Q000494:pharmacology; D002384:Catalysis; D045744:Cell Line, Tumor; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D008024:Ligands; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*
chemical_list: D000179:Acrylates; D001386:Azides; D051034:Inhibitor of Apoptosis Proteins; D008024:Ligands; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D011108:Polymers; C501628:poly(propyl acrylic acid); D003300:Copper