title: Influence of scaffold morphology on co-cultures of human endothelial and adipose tissue-derived stem cells.
abstract: The interior of tissue engineering scaffolds must be vascularizable and allow adequate nutrients perfusion in order to ensure the viability of the cells colonizing them. The promotion of rapid vascularization of scaffolds is critical for thick artificial constructs. In the present study co-cultures of human endothelial and adipose tissue-derived stem cells have been performed in poly(ethyl acrylate) scaffolds with two different pore structures: grid-like (PEA-o) or sponge-like (PEA-s), in combination with a self-assembling peptide gel filling the pores, which aims to mimic the physiological niche. After 2 and 7 culture days, cell adhesion, proliferation and migration, the expression of cell surface markers like CD31 and CD90 and the release of VEGF were assessed by means of immunocytochemistry, scanning electronic microscopy, flow cytometry and ELISA analyses. The study demonstrated that PEA-s scaffolds promoted greater cell organization into tubular-like structures than PEA-o scaffolds, and this was enhanced by the presence of the peptide gel. Paracrine signaling from adipose cells significantly improved endothelial cell viability, proving the advantageous combination of this system for obtaining easily vascularizable tissue engineered grafts. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1523-1533, 2016.
authors: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, C. de Vera s/n, Valencia, 46022, Spain.
authors: M
authors: M
authors: Arnal-Pastor
authors: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, C. de Vera s/n, Valencia, 46022, Spain.
authors: C
authors: C
authors: Martínez-Ramos
authors: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, C. de Vera s/n, Valencia, 46022, Spain.
authors: A
authors: A
authors: Vallés-Lluch
authors: Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, C. de Vera s/n, Valencia, 46022, Spain.|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, Valencia, Spain.
authors: M Monleón
authors: MM
authors: Pradas
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000273:Adipose Tissue / Q000166:cytology*; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002452:Cell Count; D002460:Cell Line; D018920:Coculture Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis; D065546:Drug Liberation; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D015703:Antigens, CD; D015415:Biomarkers; D042461:Vascular Endothelial Growth Factor A; C478961:poly(ethylacrylate); D004247:DNA
keywords: HUVECs; endothelial markers; hADSCs; scaffolds; self-assembling peptide