title: Isolation and characterization of bioactive components from Mirabilis jalapa L. radix.
abstract: The present investigation was carried out to isolate and characterize bioactive components from Mirabilis jalapa L. radix ( zǐ mò lì gēn). Thin-layer chromatography was used for the separation of spots from fractions of the crude extract. Separated spots were collected for identification of their activities. Free-radical scavenging activity was evaluated by spraying thin-layer chromatography plates (spotted with fractions) with 0.2% of 2,2-diphenyl-1-picrylhydrazyl solution. Activity against human pathogens such as Staphylococcus aureus and Candida albicans were determined using the agar diffusion method. Potential spots were subjected to infrared (IR) analysis and gas chromatography for characterization. Two spots (5F1 and 1F3) showed free-radical scavenging activity. The 1F3 spot was active against both S. aureus and C. albicans, whereas the 5F1 spot was active against S. aureus only. IR spectral analysis indicated that 5F1 spot to be a triterpenoid. Using IR spectral analysis and an IR library search, the 1F3 spot was identified to be a flavone, which may have a hydroxyl group in ring "A" of the flavone nucleus. Our results indicated that the 1F3 and 5F1 spots are potential free-radical scavengers. Both 1F3 and 5F1 exhibited antimicrobial activity. IR spectral analysis coupled with an IR library search indicated 1F3 and 5F1 to be a flavone and a triterpenoid, respectively.
authors: Pharmaceutical Technology Division, Defence Research Laboratory, Tezpur, Assam, India; Department of Life Sciences, Dibrugarh University, Dibrugarh, Assam, India.
authors: Jyotchna
authors: J
authors: Gogoi
authors: Pharmaceutical Technology Division, Defence Research Laboratory, Tezpur, Assam, India.
authors: Khonamai Sewa
authors: KS
authors: Nakhuru
authors: Pharmaceutical Technology Division, Defence Research Laboratory, Tezpur, Assam, India.
authors: Rudragoud S
authors: RS
authors: Policegoudra
authors: Pharmaceutical Technology Division, Defence Research Laboratory, Tezpur, Assam, India.
authors: Pronobesh
authors: P
authors: Chattopadhyay
authors: Department of Life Sciences, Dibrugarh University, Dibrugarh, Assam, India.
authors: Ashok Kumar
authors: AK
authors: Rai
authors: Pharmaceutical Technology Division, Defence Research Laboratory, Tezpur, Assam, India.
authors: Vijay
authors: V
authors: Veer
keywords: Bioactive components; Mirabilis jalapa,; antimicrobial; free-radical scavenging; thin-layer chromatography