title: Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling.
abstract: Osteoporosis is a bone pathology leading to increased fracture risk and challenging the quality of life. The aim of this study was to evaluate the effect of an anthraquinone glycoside, aloin, on osteogenic induction of MC3T3-E1 cells. Aloin increased alkaline phosphatase (ALP) activity, an early differentiation marker of osteoblasts. Aloin also increased the ALP activity in adult human adipose-derived stem cells (hADSC), indicating that the action of aloin was not cell-type specific.Alizarin red S staining revealed a signifiant amount of calcium deposition in cells treated with aloin. Aloin enhanced the expression of osteoblast differentiation genes, Bmp-2, Runx2 and collagen 1a, in a dose-dependent manner. Western blot analysis revealed that noggin and inhibitors of p38 MAPK and SAPK/JNK signals attenuated aloin-promoted expressions of Bmp-2 and Runx2 proteins. siRNA mediated blocking of Wnt-5a signaling pathway also annulled the influenceof aloin, indicating Wnt-5a dependent activity. Inhibition of the different signal pathways abrogated the influenceof aloin on ALP activity, confirmingthat aloin induced MC3T3-E1 cells into osteoblasts through MAPK mediated Wnt and Bmp signaling pathway.
authors: Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.|Faculty of Medical Technology, Prince of Songkla University, HatYai, Songkhla 90110, Thailand.
authors: Yutthana
authors: Y
authors: Pengjam
authors: Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
authors: Harishkumar
authors: H
authors: Madhyastha
authors: Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
authors: Radha
authors: R
authors: Madhyastha
authors: Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
authors: Yuya
authors: Y
authors: Yamaguchi
authors: Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
authors: Yuichi
authors: Y
authors: Nakajima
authors: Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
authors: Masugi
authors: M
authors: Maruyama
keywords: Aloin; Bmp signaling; MAPK; MC3T3-E1 cell; Wnt