title: Correlation of hemodynamic and fluorescence signals under resting state conditions in mice's barrel field cortex.
abstract: Both neurons and astrocytes are known to affect local vascular response in the brain following neuronal activity. In order to differentiate the contributions of each cell type to the hemodynamic response during stimulation and resting state, intrinsic optical signal (IOI) was recorded synchronized with fluorescence imaging of calcium concentration sensitive dye Oregon Green BAPTA-1 AM. By changing the stimulation parameters (frequency and duration), it was possible to individually promote neuronal and glial responses and to compare them to levels of oxy (HbO), deoxy (HbR) and total (HbT) hemoglobin concentrations. Finally, resting state recordings were done to investigate the possible correlation between hemoglobin fluctuation and calcium transients, based on different frequency bands associated either with neuronal or glial activity.
authors: Institut de Génie Biomédical, Dpt. de Génie Électrique, École Polytechnique de Montréal, C.P. 6079, succ. Centre-ville, Montréal, QC H3C 3A7, Canada; Montreal Heart Institute, 5000 rue Bélanger, Montréal, QC H1T 1C8, Canada. Electronic address: samuel.belanger@polymtl.ca.
authors: Samuel
authors: S
authors: Bélanger
authors: École d'Optométrie, Université de Montréal, 3744 Jean-Brillant, Montréal, QC H3T 1P1, Canada.
authors: Bruno Oliveira Ferreira
authors: BO
authors: de Souza
authors: École d'Optométrie, Université de Montréal, 3744 Jean-Brillant, Montréal, QC H3T 1P1, Canada.
authors: Christian
authors: C
authors: Casanova
authors: Institut de Génie Biomédical, Dpt. de Génie Électrique, École Polytechnique de Montréal, C.P. 6079, succ. Centre-ville, Montréal, QC H3C 3A7, Canada; Montreal Heart Institute, 5000 rue Bélanger, Montréal, QC H1T 1C8, Canada.
authors: Frédéric
authors: F
authors: Lesage
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism*; D005452:Fluoresceins; D005453:Fluorescence; D005456:Fluorescent Dyes; D006439:Hemodynamics; D006454:Hemoglobins / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D012146:Rest
chemical_list: D000814:Aniline Compounds; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006454:Hemoglobins; C503942:Oregon green 488 BAPTA-1; D010108:Oxyhemoglobins; C100003:deoxyhemoglobin; D002118:Calcium
keywords: Astrocytes; Barrel field cortex; Calcium fluorescence signal imaging; Intrinsic signal imaging; Neurovascular coupling; Resting state