title: Use of Mesenchymal Stem Cell-Conditioned Medium to Activate Islets in Preservation Solution.
abstract: Pancreatic islet transplantation has received widespread attention as a promising treatment for type 1 diabetes. However, islets for transplantation are subject to damage from a number of sources, including ischemic injury during removal and delivery of the donor pancreas, enzymatic digestion during islet isolation, and reperfusion injury after transplantation in the recipient. Here we found that protein fractions secreted by mesenchymal stem cells (MSCs) were capable of activating preserved islets. A conditioned medium from the supernatant obtained by culturing adipose tissue MSCs (derived from wild-type Lewis rats) was prepared for 2 days in serum-free medium. Luc-Tg rat islets to which an organ preservation solution was added were then incubated at 4°C with fractions of various molecular weights prepared from the conditioned medium. Under the treatment with some of the fractions, by 4 days the relative luminescence intensities (representative of the ATP levels of the cold-preserved islets) had increased to over 150% of their initial values. Our novel system may be able to restore isolated islets to the condition they were in before transport, culture, and transplantation.
authors: Division of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsukeshi, Tochigi, Japan; †Department of Surgery, Jichi Medical University, Shimotsukeshi, Tochigi, Japan.
authors: Naoya
authors: N
authors: Kasahara
authors: Division of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
authors: Takumi
authors: T
authors: Teratani
authors: Division of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
authors: Junshi
authors: J
authors: Doi
authors: Division of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
authors: Yuki
authors: Y
authors: Iijima
authors: Division of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
authors: Masashi
authors: M
authors: Maeda
authors: ‡ Division of Hepato-Pancreato-Biliary Surgery and Transplantation, Department of Surgery, Kyoto University Graduate School of Medicine , Syougoin, Sakyoku, Kyotoshi, Kyoto , Japan.
authors: Shinji
authors: S
authors: Uemoto
authors: Division of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsukeshi, Tochigi, Japan; ‡Division of Hepato-Pancreato-Biliary Surgery and Transplantation, Department of Surgery, Kyoto University Graduate School of Medicine, Syougoin, Sakyoku, Kyotoshi, Kyoto, Japan.
authors: Yasuhiro
authors: Y
authors: Fujimoto
authors: † Department of Surgery, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
authors: Naohiro
authors: N
authors: Sata
authors: † Department of Surgery, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
authors: Yoshikazu
authors: Y
authors: Yasuda
authors: Division of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
authors: Eiji
authors: E
authors: Kobayashi
keywords: Living image; Mesenchymal stem cells (MSCs); Preservation of islets; Transgenic rat