title: Krüppel-like factor 2 promotes cell proliferation in hepatocellular carcinoma through up-regulation of c-myc.
abstract: Members of krüppel-like factor family have been shown to play critical roles in the development, metabolism and tumorigenesis. Our previous study demonstrated that up-regulationupregulation of KLF2 in livers from obese mice could promote hepatosteatosis. However, little is known about its effects in hepatocellular carcinogenesis. In the present study, we found that mRNA and protein expression of KLF2 was increased in primary HCC, when compared with that in adjacent normal liver. In vitro studies further showed that enforced overexpression of KLF2 expression enhanced, while its knockdown inhibited cell proliferation and invasion. At the molecular level, c-myc was a direct transcriptional target of KLF2 and a KLF2-binding site in the c-myc promoter bound specifically to KLF2 protein. Indeed, ablation of c-myc largely attenuated the proliferative roles of KLF2 in HCC cells. Therefore, our data highlight an important role for KLF2/c-myc pathway in HCC development and progression.
authors: a Department of Gastroenterology , Shanghai East Hospital, Tongji University School of Medicine , Shanghai , 200120 , P.R. China.
authors: Kailin
authors: K
authors: Zou
authors: a Department of Gastroenterology , Shanghai East Hospital, Tongji University School of Medicine , Shanghai , 200120 , P.R. China.
authors: Xiaojie
authors: X
authors: Lu
authors: a Department of Gastroenterology , Shanghai East Hospital, Tongji University School of Medicine , Shanghai , 200120 , P.R. China.
authors: Kun
authors: K
authors: Ye
authors: a Department of Gastroenterology , Shanghai East Hospital, Tongji University School of Medicine , Shanghai , 200120 , P.R. China.
authors: Chunmei
authors: C
authors: Wang
authors: a Department of Gastroenterology , Shanghai East Hospital, Tongji University School of Medicine , Shanghai , 200120 , P.R. China.
authors: Tiangeng
authors: T
authors: You
authors: b Department of Gastroenterology , Fengxian Hospital, Southern Medical University , Shanghai , 200000 , P.R. China.|c Department of Gastroenterology , Shanghai Sixth People's Hospital (South), Shanghai Jiaotong University School of Medicine, Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine , Shanghai , 200120 , P.R. China.|d Department of Gastroenterology , Shanghai East Hospital, Tongji University School of Medicine , Shanghai , 200120 , P.R. China.
authors: Jinlian
authors: J
authors: Chen
mesh_terms: D000818:Animals; D001665:Binding Sites; D063646:Carcinogenesis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction
chemical_list: C096161:KLF2 protein, human; D051741:Kruppel-Like Transcription Factors; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger
keywords: Cell proliferation; KLF2; c-myc; hepatocellular carcinoma; transcription