title: Simultaneous Photoelectrochemical Immunoassay of Dual Cardiac Markers Using Specific Enzyme Tags: A Proof of Principle for Multiplexed Bioanalysis.
abstract: In this Letter, on the basis of the CdS quantum dots functionalized TiO2 nanotubes electrode, we proposed a simultaneous photoelectrochemical (PEC) immunoassay of dual cardiac markers using specific enzyme tags of alkaline phosphatase (ALP) and acetylcholine esterase (AChE). ALP and AChE were integrated into the PEC system through the sandwich immunobinding and could specifically catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) or the acetylthiocholine (ATC) to in situ generate ascorbic acid (AA) or thiocholine (TC) for sacrificial electron donating. These two enzymes were thus used to differentiate the signals of two cardiac targets in connection with the sandwich immunorecognition and PEC responses to the corresponding electron donors. This strategy demonstrates a proof of principle for the successful integration of dual enzyme tags with PEC immunoassay that can potentially provide a general format for multiplexed PEC bioanalysis.
authors: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, China.
authors: Nan
authors: N
authors: Zhang
authors: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, China.
authors: Zheng-Yuan
authors: ZY
authors: Ma
authors: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, China.
authors: Yi-Fan
authors: YF
authors: Ruan
authors: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, China.
authors: Wei-Wei
authors: WW
authors: Zhao
authors: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, China.
authors: Jing-Juan
authors: JJ
authors: Xu
authors: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210023, China.
authors: Hong-Yuan
authors: HY
authors: Chen
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000122:Acetylthiocholine / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D002097:C-Reactive Protein / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D007118:Immunoassay; D043942:Nanotubes / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry; D014025:Titanium / Q000737:chemistry; D019210:Troponin I / Q000032:analysis
chemical_list: D055910:Antibodies, Immobilized; D019210:Troponin I; C009495:titanium dioxide; C011669:ascorbate-2-phosphate; D000122:Acetylthiocholine; D002097:C-Reactive Protein; D014025:Titanium; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid