title: Linear antigenic mapping of flagellin (FliC) from Salmonella enterica serovar Enteritidis with yeast surface expression system.
abstract: Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major cause of food-borne illness around the world and can have significant health implications in humans, poultry and other animals. Flagellin (FliC) is the primary component of bacterial flagella. It has been shown that the FliC of S. Enteritidis is a significant antigenic structure and can elicit strong humoral responses against S. Enteritidis infection in chickens. Here, we constructed a FliC antigen library using a yeast surface expression system. Yeast cells expressing FliC peptide antigens were labeled with chicken sera against S. Enteritidis and sorted using FACS. The analyses of FliC peptides revealed that the FliC linear antigenicity in chickens resided on three domains which were able to elicit strong humoral responses in vivo. Animal experiments further revealed that the antibodies elicited by these antigenic domains were able to significantly inhibit the invasion of S. Enteritidis into the liver and spleen of chickens. These findings will facilitate our better understanding of the humoral responses elicited by FliC in chickens upon infection by S. Enteritidis.
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors: Gaoling
authors: G
authors: Wang
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors: Bingtian
authors: B
authors: Shi
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors: Tao
authors: T
authors: Li
authors: Comprehensive AIDS Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.
authors: Teng
authors: T
authors: Zuo
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China; College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors: Bin
authors: B
authors: Wang
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors: Wei
authors: W
authors: Si
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
authors: Jiuqing
authors: J
authors: Xin
authors: Neurosurgery Department of the First Affiliated Hospital, Harbin Medical University, China.
authors: Kongbin
authors: K
authors: Yang
authors: Comprehensive AIDS Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.
authors: Xuanlin
authors: X
authors: Shi
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China. Electronic address: siguo_liu@hvri.ac.cn.
authors: Siguo
authors: S
authors: Liu
authors: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China. Electronic address: lhg@hvri.ac.cn.
authors: Henggui
authors: H
authors: Liu
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D002645:Chickens / Q000276:immunology* / Q000382:microbiology; D018604:Epitope Mapping; D005408:Flagellin / Q000235:genetics* / Q000276:immunology*; D015723:Gene Library; D056724:Immunity, Humoral; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology; D012477:Salmonella enteritidis / Q000235:genetics* / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D005408:Flagellin
keywords: Antigenic domain; FliC; Salmonella enterica serovar Enteritidis; Yeast surface expression system