title: [The forensic chemical investigation of acetylsalicylic acid].
abstract: The objective of the present study was to develop the universal approach to the quantitative determination of acetylsalicylic acid in biological tissues and fluids to be applied in the practice of forensic chemical expertise with the use of thin-layer chromatography, gas chromatography and mass spectrometry, low-pressure column chromatography, and spectrophotometry. A system of solvents consisting of acetone and ethyl acetate (7:3) was proposed as a universal agent for extracting acetylsalicylic acid from the cadaveric tissues and blood. It was shown that acetylsalicylic acid and its principal metabolite, salicylic acid, can be purified from the endogenous admixtures present in the biological materials by column chromatography on silica gel L 40/100 mcm. Salicylic acid in extracts from biological materials was identified and quantified with the use of thin-layer chromatography, gas chromatography/mass spectrometry, and electronic spectrophotometry. The method for forensic chemical investigation of acetylsalicylic acid has been developed and applied in the analysis of the material provided for expertise.
authors: Kursk State University, Kursk, Russia, 305041.
authors: V K
authors: VK
authors: Shormanov
authors: Orel State University, Orel, Russia, 302028.
authors: V V
authors: VV
authors: Chupak
authors: Kursk State University, Kursk, Russia, 305041.
authors: M N
authors: MN
authors: Pobedonstseva
authors: Kursk Regional Bureau of Forensic Medical Expertise, Kursk, Russia, 305000.
authors: S V
authors: SV
authors: Maslov
authors: Kursk State University, Kursk, Russia, 305041.
authors: N A
authors: NA
authors: Kibets
authors: Kursk Regional Bureau of Forensic Medical Expertise, Kursk, Russia, 305000.
authors: N N
authors: NN
authors: Tikhopoeva
mesh_terms: D000096:Acetone / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology; D001241:Aspirin / Q000737:chemistry / Q000494:pharmacology; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000473:pathology; D008099:Liver / Q000737:chemistry / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods; D009928:Organ Specificity; D015203:Reproducibility of Results; D012997:Solvents / Q000494:pharmacology; D013053:Spectrophotometry / Q000379:methods; D013270:Stomach / Q000737:chemistry / Q000473:pathology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D012997:Solvents; D000096:Acetone; D001241:Aspirin