title: Occurrence and antimicrobial resistance of emergent Arcobacter spp. isolated from cattle and sheep in Iran.
abstract: This study is conducted to determine the occurrence and antimicrobial resistance of Arcobacter spp. isolated from clinically healthy food animals. A total of 308 samples from cattle (200) and sheep (108) were collected from Shiraz slaughterhouse, southern Iran to investigate the presence of the important Arcobacter spp. using cultivation and Polymerase Chain Reaction (PCR) methods. Antimicrobial susceptibility of Arcobacter isolates was determined for 18 antibiotics using disk diffusion method. Among 308 samples, 27 (8.7%) and 44 (14.28%) were positive for the presence of Arcobacter species with cultivation and PCR procedures, respectively. The predominant species was A. butzleri in both cattle (58.33%) and sheep (55%). In addition, concurrent incidence of the species was observed in 25% of the positive samples. All Arcobacter isolates were resistant to rifampicin, vancomycin, ceftriaxone, trimethoprim and cephalothin. The isolates showed high susceptibility to tetracycline, oxytetracycline, erythromycin, ciprofloxacin, kanamycin, amikacin, gentamicin and enrofloxacin. No significant difference among cattle and sheep isolates in resistance pattern was observed. The results indicate that cattle and sheep are significant intestinal carriers for Arcobacter spp. Moreover, tetracycline and aminoglycosides showed great effects on Arcobacter species in antibiogram test and can be used for treatment of human Arcobacter infections.
authors: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors: Hesamaddin
authors: H
authors: Shirzad Aski
authors: Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors: Mohammad
authors: M
authors: Tabatabaei
authors: Department of Food hygiene and Public Health, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
authors: Rahem
authors: R
authors: Khoshbakht
authors: Cereal Health Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Public Health, Golestan University of Medical Sciences, Gorgan, Iran. Electronic address: drmraeisi@goums.ac.ir.
authors: Mojtaba
authors: M
authors: Raeisi
mesh_terms: D000003:Abattoirs; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D020640:Arcobacter / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002353:Carrier State / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002939:Ciprofloxacin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D007492:Iran / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D034561:Sheep, Domestic / Q000382:microbiology; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D013752:Tetracycline
keywords: Antimicrobial susceptibility; Arcobacter; Cattle; Sheep