title: In vitro antimicrobial susceptibility of Mycoplasma bovis clinical isolates recovered from bison (Bison bison).
abstract: Mycoplasma bovis is a pathogen globally affecting cattle and bison herds, causing pneumonia, arthritis, mastitis, abortions, and other symptoms, leading to huge economic losses. Many studies have been done regarding the antimicrobial susceptibility of M. bovis isolated from cattle, but no such study is available for isolates recovered from bison. For the first time, in vitro susceptibilities of 40 M. bovis clinical isolates collected from bison herds in Canada are reported here. Minimal inhibitory concentration (MIC) values were determined using Sensititre® plates. The most effective MIC50 and MIC90 were for spectinomycin (1 and >64 μg/mL), tiamulin (1 and >32 μg/mL), and tulathromycin (16 and 64 μg/mL), whereas tetracyclines, fluoroquinolones, and florfenicol failed to inhibit growth of M. bovis bison isolates. Isolates were nonsusceptible to tetracyclines (100%), fluoroquinolones (97.5%), and tilmicosin (100%), whereas the highest susceptibility of bison clinical isolates was seen with spectinomycin (95%) and tulathromycin (67.5%). Two lung isolates (Mb283 and 348) were found resistant to both spectinomycin and tulathromycin. These results show a marked difference in antimicrobial susceptibility of bison isolates as compared with previously reported and laboratory reference cattle isolates, emphasizing the necessity of testing antimicrobial susceptibility of M. bovis bison isolates and to generate better therapeutic regime for improved recovery chances for infected bison herds across North America.
authors: a Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada.
authors: Muhammad
authors: M
authors: Suleman
authors: a Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada.
authors: Tracy
authors: T
authors: Prysliak
authors: b Department of Production Animal Health, University of Calgary, Faculty of Veterinary Medicine, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
authors: Claire
authors: C
authors: Windeyer
authors: a Vaccine and Infectious Disease Organization - International Vaccine Centre, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada.
authors: Jose
authors: J
authors: Perez-Casal
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D016164:Bison / Q000382:microbiology*; D002417:Cattle / Q000382:microbiology; D005260:Female; D008826:Microbial Sensitivity Tests; D045682:Mycoplasma bovis / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents
keywords: Mycoplasma bovis; antimicrobial susceptibility; bison; concentration minimale inhibitrice; minimal inhibitory concentration; susceptibilité aux antimicrobiens