title: miR-135b inhibits tumour metastasis in prostate cancer by targeting STAT6.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that participate in several cellular functions and tumour progression. A previous microarray study demonstrated that miR-135b is downregulated in prostate cancer (PCa) cells, but the role and molecular mechanism of miR-135b in the regulation of tumour metastasis remain to be elucidated. In the present study, significant downregulation of miR-135b in PCa tissues, compared with noncancerous tissues, was detected by reverse transcription-quantitative polymerase chain reaction. Furthermore, the expression of miR-135b was demonstrated to be associated with the pathological stage and the levels of total and free prostate-specific antigen (PSA) in PCa cells. In addition, signal transducer and activator of transcription 6 (STAT6) was identified as a target of miR-135b in PCa cells by luciferase activity and western blot assays. The upregulation of miR-135b in PCa cells led to reduced expression of STAT6 in the cytoplasm and nucleus of these cells, while the overexpression of miR-135b and knockdown ofSTAT6were able to inhibit the migration and invasion abilities of PCa cellsin vitro. Therefore, the results of the present study indicate that miR-135b suppresses tumour metastasis by targeting STAT6.
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Ning
authors: N
authors: Wang
authors: Department of Urology and Institute of Prostatic Diseases, The Affiliated Wuhu No. 2 People's Hospital of Wannan Medical College, Wuhu, Anhui 241000, P.R. China.
authors: Liangjun
authors: L
authors: Tao
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Huan
authors: H
authors: Zhong
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Sihai
authors: S
authors: Zhao
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Ying
authors: Y
authors: Yu
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Bin
authors: B
authors: Yu
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Xiaonong
authors: X
authors: Chen
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Jianguo
authors: J
authors: Gao
authors: Department of Urology, The First Affiliated Hospital of Huzhou Teachers College, Huzhou, Zhejiang 313000, P.R. China.
authors: Rongjiang
authors: R
authors: Wang
keywords: STAT6; metastasis; miR-135b; prostate cancer