title: Endothelial differentiation of canine yolk sac cells transduced with VEGF.
abstract: Yolk sac (YS) is the site of blood-cell production where primitive erythroid cells originate and complete their maturation. YS is a source of precursor cells, however its differentiation potential and suitability for cell therapies are not well described. YS can be a cell source when neovascularization is required. This study characterized YS canine cells, transduced with VEGF, to analyze then using Immunocytochemistry, flow cytometry and real time PCR. Immunocytochemistry: positive expression for CD105, PCNA, VEGF and vWF, flow cytometry for CD105, VEGF, PCNA, OCT-4 and RT-qPCR for VEGF, CD31, CD105, PCNA and FLT - 1, indicating that these cells have characteristics of endothelial progenitor and pluripotency. After transduction, the YS cells changed their morphology and showed endothelial-like cells. We suggest, because of their cell surface phenotype as well as their capacity to differentiate into endothelial-like cells, that canine YS represents a source of cells for neovascularization therapies.
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. Electronic address: fratini@usp.br.
authors: Paula
authors: P
authors: Fratini
authors: School of Medicine, NUCEL (Cell and Molecular Therapy Center) and NETCEM (Center for Studies in Cell and Molecular Therapy), Medical Clinics Department, University of São Paulo, Brazil.
authors: Ana Claudia Oliveira
authors: AC
authors: Carreira
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors: Dayane
authors: D
authors: Alcântara
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors: Fernanda Menezes
authors: FM
authors: de Oliveira e Silva
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors: Marcio Nogueira
authors: MN
authors: Rodrigues
authors: Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors: Maria Angelica
authors: MA
authors: Miglino
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004285:Dogs / Q000196:embryology* / Q000378:metabolism; D005434:Flow Cytometry / Q000662:veterinary; D013234:Stem Cells / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D015017:Yolk Sac / Q000196:embryology*
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: Angiogenesis; Cell therapy; VEGF; Vascularization; Yolk sac