title: [Structural and Functional Organization of the Signal Peptide of ProEnterotoxin B from Staphylococcus aureus].
abstract: A series of genes of proenterotoxin B from Staphylococcus aureus containing signal peptide mutant forms was constructed in order to study the functional roles of the introduced mutations. It was shown that a continuous mutation in the n-region of the signal peptide does not affect the secretion efficiency of proenterotoxin B, in contrast to the analogous mutation in the h-region. Point mutations of the proprotein signal peptide, including the N-terminal amino-acid residue of the mature protein, were obtained. It was shown that the introduced structural. changes cause a decrease in secretion efficiency and a redistribution of the protein in various compartments of Escherichia coli cells.
authors: N N
authors: NN
authors: Mordkovich
authors: N A
authors: NA
authors: Okorokova
authors: V P
authors: VP
authors: Veiko
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D004768:Enterotoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D019897:Periplasm / Q000737:chemistry / Q000378:metabolism; D017354:Point Mutation; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D004768:Enterotoxins; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C031818:enterotoxin B, staphylococcal