title: The immunotoxicity of aluminum trichloride on rat peritoneal macrophages via β2-adrenoceptors/cAMP pathway acted by norepinephrine.
abstract: The previous research found that norepinephrine (NE) enhanced the immunotoxicity of aluminum trichloride (AlCl3) on rat peritoneal macrophages in vitro through activating the β2-adrenoceptors (β2-AR)/cAMP pathway. On that basis, the experiment in vivo was conducted in this experiment. Eighty Wistar rats were orally exposed to 0 (control group); 0.4 mg/mL (low-dose group); 0.8 mg/mL (mid-dose group) and 1.6 mg/mL (high-dose group) AlCl3 for 120 days, respectively. Aluminum (Al), NE, macrophage migration inhibitory factor (MIF) and tumor necrosis factor-α (TNF-α) contents in serum, cAMP content, β2-AR density, mRNA expressions of TNF-α, MIF and β2-AR in rat peritoneal macrophages were examined. These results showed that AlCl3 increased serum Al and NE contents, peritoneal macrophages cAMP content, the density and mRNA expression of the β2-AR, and decreased serum MIF and TNF-α contents, peritoneal macrophages mRNA expressions of MIF and TNF-α. Serum NE content was negatively correlated with serum TNF-α and MIF contents and peritoneal macrophages mRNA expressions of TNF-α and MIF, but positively correlated with cAMP content, density of β2-AR and mRNA expression of β2-AR of peritoneal macrophages. It indicated that AlCl3 suppresses peritoneal macrophages function of rats through β2-AR/cAMP pathway acted by NE.
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Cuicui
authors: C
authors: Zhuang
authors: Heilongjiang Province Hospital, Harbin 150036, China; School Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors: Dawei
authors: D
authors: Liu
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Xu
authors: X
authors: Yang
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors: Haoran
authors: H
authors: Wang
authors: ICareVet Pet Hospital, Shenyang 110014, China.
authors: Lulu
authors: L
authors: Han
authors: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: yanfeili_200@126.com.
authors: Yanfei
authors: Y
authors: Li
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides / Q000633:toxicity*; D019746:Intramolecular Oxidoreductases; D008263:Macrophage Migration-Inhibitory Factors; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011941:Receptors, Adrenergic; D022702:Receptors, Adrenergic, beta-3 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D008263:Macrophage Migration-Inhibitory Factors; D011941:Receptors, Adrenergic; D022702:Receptors, Adrenergic, beta-3; D014409:Tumor Necrosis Factor-alpha; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000077410:Aluminum Chloride; C044258:8-chloro-cyclic adenosine monophosphate; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human; D009638:Norepinephrine
keywords: Aluminum trichloride; Noradrenaline; Peritoneal macrophage; Rat; β(2)-AR/cAMP pathway