title: Spectrofluorometric Determination of Putrescine Using Orthophthaldehyde as a Fluorophore: A Novel Method.
abstract: This paper concerns spectrofluorometric analysis of putrescine using orthophthaladehyde as a fluorophore in aqueous alkaline medium. Wavelengths of excitation and emission in acid, neutral, and alkaline media were different. There is a maximum intensity of fluorescence in alkaline medium 24 h after complexation compared with other media. Putrescine and orthophthaladehyde are used at an equimolar ratio, and the product is kinetically stable in alkaline medium. Calibration curves obtained gave limits of detection and quantification of 39 and 65 ng/mL, respectively. The correlation coefficient obtained in alkaline medium was 0.992 at pH 12. Results obtained largely showed a good reproducibility of our method.
authors: University of The Gambia, School of Arts and Sciences, Division of Physical and Natural Sciences, Gambia.
authors: Oladele
authors: O
authors: Oyelakin
authors: Moumouny
authors: M
authors: Traoré
authors: El Hadji Babacar
authors: el HB
authors: Mbaye
authors: Abdourahmane
authors: A
authors: Konté
authors: Lamine
authors: L
authors: Cissé
authors: Abdoulaye N
authors: AN
authors: Faye
authors: Ousman
authors: O
authors: Faye
authors: Moussa
authors: M
authors: Mbaye
authors: Khemesse
authors: K
authors: Kital
authors: Gaye
authors: G
authors: Seye
authors: Atanasse
authors: A
authors: Coly
authors: Alphonse
authors: A
authors: Tine
authors: François
authors: F
authors: Delattre
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D011700:Putrescine / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D009764:o-Phthalaldehyde / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009764:o-Phthalaldehyde; D011700:Putrescine