title: GRHL2 genetic polymorphisms may confer a protective effect against sudden sensorineural hearing loss.
abstract: Genetic polymorphisms in grainyhead‑like 2 (GRHL2) variants were examined for their suspected association with sudden sensorineural hearing loss (SSHL). Between January 2009 and April 2014, 190 patients with SSHL, who were diagnosed at the Departments of Otorhinolaryngology Head and Neck Surgery at Kaihua People's Hospital and Hangzhou First People's Hospital, were selected for the present study and defined as the SSHL group. A group of 210 healthy individuals were defined as the control group. Polymerase chain reaction (PCR)‑restriction fragment length polymorphism was used to detect GRHL2 genotypes, using genomic DNA isolated from peripheral blood as PCR templates. GRHL2 rs611419 genetic polymorphisms conferred a protective effect against SSHL (AT+TT vs. AA: OR=0.63, 95% CI=0.41‑0.98, P=0.038). In addition, rs10955255 polymorphisms were associated with a reduced risk of SSHL (AA vs. GG: OR=0.54, 95% CI=0.31‑0.95, P=0.032; GA+AA vs. GG: OR=0.58, 95% CI=0.38‑0.89, P=0.012). Combined genotypes of rs611419, rs10955255 and rs6989650 in the GRHL2 gene are also associated with a reduced risk of SSHL (P=0.035). In subjects who consumed alcohol, co‑occurrence of 3‑8 variant alleles conferred increased resistance to SSHL, compared with the occurrence of 0‑2 variant alleles (OR=0.40, 95% CI=0.21‑0.76, P=0.004). GRHL2 genetic polymorphisms, rs611419 and rs10955255, have a protective role against SSHL and reduce the risk of SSHL. However, rs6989650 is not associated with SSHL.
authors: Department of Otorhinolaryngology Head and Neck Surgery, Kaihua People's Hospital, Quzhou, Zhejiang 324300, P.R. China.
authors: Xiaojiang
authors: X
authors: Lin
authors: Department of Otorhinolaryngology Head and Neck Surgery, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, P.R. China.
authors: Yaoshu
authors: Y
authors: Teng
authors: Department of Otorhinolaryngology, Quzhou People's Hospital, Quzhou, Zhejiang 324300, P.R. China.
authors: Jinshan
authors: J
authors: Lan
authors: Department of Otorhinolaryngology Head and Neck Surgery, Kaihua People's Hospital, Quzhou, Zhejiang 324300, P.R. China.
authors: Benjun
authors: B
authors: He
authors: Department of Otorhinolaryngology Head and Neck Surgery, Kaihua People's Hospital, Quzhou, Zhejiang 324300, P.R. China.
authors: Huijuan
authors: H
authors: Sun
authors: Department of Otorhinolaryngology Head and Neck Surgery, Kaihua People's Hospital, Quzhou, Zhejiang 324300, P.R. China.
authors: Fenglin
authors: F
authors: Xu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D060467:Disease Resistance; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006319:Hearing Loss, Sensorineural / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D020641:Polymorphism, Single Nucleotide; D065840:Protective Factors; D012306:Risk; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C470830:GRHL2 protein, human; D014157:Transcription Factors