title: The Effect of Carbon Dioxide Insufflation Applied at Different Pressures and Periods on Thrombotic Factors.
abstract: The aim of this experimantal study which is applied on rats, is to determine the differences on the clotting factors over the application of low and high intraabdominal pressure (IAP) values in different periods of time in carbon dioxide (CO2) pneumoperitoneum. Thirty rats were randomized into five groups (n = 6): a control group (Group K) and 1 h and 6 mm Hg IAP (Group A), 2 h and 6 mm Hg IAP (Group B), 1 h and 12 mm Hg IAP (Group C) and 2 h and 12 mm Hg IAP were created with CO2 pneumoperitoneum (Group D). At the end of the experiment, plasma samples taken from subjects and fibrinogen, FII (prothrombin), FV, FVII, FVIII, FIX, FX, FXI, FXII, von willebrand's factor (vWF), ristocetin cofactor, protein C, protein S, antithrombin III (AT III) levels are studied. There were statistically significant differences in the mean levels of FII, FV, FVII, FVIII, FIX, FX, FXI, FXII, and protein S between the groups. A hypercoagulable state occurred with the following: increase in the coagulation parameters compared to the control group; increase in FVII in the group only Group C; decrease in AT III in all groups compared to the control group; decrease in protein C in the group only XII Group D compared to control group; decrease in protein S in all groups except group D compared to control group. CO2 insufflation predisposes to thromboembolic events both by inducing coagulation factors and by suppressing the fibrinolytic system contrary to the controversies in the literature.
authors: Department of Pediatric Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Mehmet Celal
authors: MC
authors: Sen
authors: Department of Pediatric Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Zafer
authors: Z
authors: Turkyilmaz
authors: Department of Pediatric Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Kaan
authors: K
authors: Sonmez
authors: Department of Pediatric Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Ramazan
authors: R
authors: Karabulut
authors: Department of Pediatric Hematology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Zuhre
authors: Z
authors: Kaya
authors: Department of Pediatric Hematology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Idil
authors: I
authors: Yenicesu
authors: Department of Pediatric Hematology, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Turkiz
authors: T
authors: Gursel
authors: Department of Pediatric Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey.
authors: Abdullah Can
authors: AC
authors: Basaklar
keywords: Carbon dioxide; Coagulation parameters; Pneumoperitoneum; Rats