title: An electrochemical aptasensor electrocatalyst for detection of thrombin.
abstract: This work reports a novel signal amplification strategy based on three-dimensional ordered macroporous C60-poly(3,4-ethylenedioxythiophene)-1-butyl-3-methylimidazolium hexafluorophosphate (3DOM C60-PEDOT-[BMIm][BF6]) for ultrasensitive detection of thrombin by cascade catalysis of Au-PEDOT@SiO2 microspheres and alcohol dehydrogenase (ADH). Au-PEDOT@SiO2 microspheres were constructed not only as nanocarriers to anchor the large amounts of secondary thrombin aptamers but also as nanocatalysts to catalyze the oxidation of ethanol efficiently. Significantly, the electrochemical signal was greatly enhanced based on cascade catalysis: First, ADH catalyzed the oxidation of ethanol to acetaldehyde with the concomitant generation of NADH in the presence of β-nicotinamide adenine dinucleotide hydrate (NAD(+)). Then, gold nanoparticles (AuNPs) as nanocatalysts could effectively catalyze NADH to produce NAD(+) with the help of PEDOT as redox probe. Under the optimal conditions, the proposed aptasensor exhibits a linear range of 2 × 10(-13) to 2 × 10(-8) M with a low detection limit of 2 × 10(-14) M for thrombin detection and shows high sensitivity and good specificity.
authors: College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China; Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, People's Republic of China. Electronic address: t6865233@126.com.
authors: Rong
authors: R
authors: Tian
authors: College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
authors: Xiaojun
authors: X
authors: Chen
authors: Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, People's Republic of China.
authors: Qingwen
authors: Q
authors: Li
authors: College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China. Electronic address: yaocheng@njtech.edu.cn.
authors: Cheng
authors: C
authors: Yao
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002138:Calibration; D002384:Catalysis; D055664:Electrochemical Techniques / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin
keywords: 3DOM; Aptasensor; Au-PEDOT@SiO(2); C(60)-PEDOT-[BMIm][BF(6)]; TB