title: Protein regulation of induced pluripotent stem cells by transplanting in a Huntington's animal model.
abstract: The purpose of this study was to determine the functional recovery and protein regulation by transplanted induced pluripotent stem cells in a rat model of Huntington's disease (HD).
  
  
  
  In a quinolinic acid-induced rat model of striatal degeneration, induced pluripotent stem cells were transplanted into the ipsilateral lateral ventricle 10 days after the quinolinic acid injection. At 8 weeks after transplantation, fluorodeoxyglucose-PET/CT scan and balance-beam test were performed to evaluate the functional recovery of experimental rats. In addition, immunofluorescence and protein array analysis were used to investigate the regulation of stimulated protein expression in the striatum.
  
  
  
  At 8 weeks after induced pluripotent stem cell transplantation, motor function was improved in comparison with the quinolinic acid-treated rats. High fluorodeoxyglucose accumulation in the injured striatum was also observed by PET/CT scans. In addition, immunofluorescence analysis demonstrated that implanted cells migrated from the lateral ventricle into the lesioned striatum and differentiated into striatal projection neurons. Array analysis showed a significant upregulation of GFR (Glial cell line-derived neurotrophic factor receptor) alpha-1, Adiponectin/Acrp30, basic-fibroblast growth factors, MIP-1 (Macrophage-inflammatory protein) alpha and leptin, as well as downregulation of cytokine-induced neutrophil chemoattractant-3 in striatum after transplantatation of induced pluripotent stem cells in comparison with the quinolinic acid -treated rats.
  
  
  
  The findings in this work indicate that transplantation of induced pluripotent stem cells is a promising therapeutic candidate for HD.
authors: Psychology & Social College of Shenzhen University, Shenzhen, China.
authors: S
authors: S
authors: Mu
authors: School of Medicine, Shenzhen University, Shenzhen, China.
authors: L
authors: L
authors: Han
authors: School of Medicine, Shenzhen University, Shenzhen, China.
authors: G
authors: G
authors: Zhou
authors: School of Medicine, Shenzhen University, Shenzhen, China.
authors: C
authors: C
authors: Mo
authors: School of Medicine, Shenzhen University, Shenzhen, China.
authors: J
authors: J
authors: Duan
authors: School of Medicine, Shenzhen University, Shenzhen, China.
authors: Z
authors: Z
authors: He
authors: Department of Neurology, Shenzhen Shekou People's Hospital, Shenzhen, China.
authors: Z
authors: Z
authors: Wang
authors: Department of Neurology, Shenzhen Second People's Hospital (First Affiliated Hospital of Shenzhen University), Shenzhen, China.
authors: L
authors: L
authors: Ren
authors: School of Medicine, Shenzhen University, Shenzhen, China. jzhanghappy@163.com.
authors: J
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D004195:Disease Models, Animal; D064593:Heterografts; D006816:Huntington Disease; D057026:Induced Pluripotent Stem Cells / Q000637:transplantation*; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D011506:Proteins
keywords: Huntington's disease; induced pluripotent stem cells; projection neurons; quinolinic acid