title: Influence of SKF38393 on changes of gene profile in rat prefrontal cortex during chronic paradoxical sleep deprivation.
abstract: Chronic paradoxical sleep deprivation (CSD) can induce dramatic physiological and neurofunctional changes in rats, including decreased body weight, reduced learning and memory, and declined locomotor function. SKF38393, a dopamine D1 receptor agonist, can reverse the above damages. However, the mechanism of CSD syndrome and reversal role of SKF38393 remains largely unexplained. To preliminarily elucidate the mechanism of the neural dysfunction caused by CSD, in the present study we use gene chips to examine the expression profile of more than 28,000 transcripts in the prefrontal cortex (PFC). Rats were sleep deprived by modified multi-platform method for 3 weeks. Totally 59 transcripts showed differential expressions in CSD group in contrast to controls; they included transcripts coding for caffeine metabolism, circadian rhythm, drug metabolism and some amino acid metabolism pathway. Among the 59 transcripts, 39 increased their expression and 20 decreased. Two transcripts can be specifically reversed with SKF38393, one of them is Homer1, which is related to 20 functional classifications and coding for Glutamatergic synapse pathway. Our findings in the present study indicate that long-term sleep deprivation may trigger the changes of some certain functions and pathways in the PFC, and lead to the dysfunction of this advanced neuron, and the activation of D1 receptor by SKF38393 might ameliorate these changes via modulation of some transcripts such as Homer1, which is involved in the Ca(2+) pathway and MAPK pathway related to Glutamatergic synapse pathway.
authors: Department of Enviromental Hygiene, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China; Minhang District Center for Disease Control and Prevention, Shanghai 201101, China.
authors: Xiaosa
authors: X
authors: Wen
authors: Department of Enviromental Hygiene, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China.
authors: Xinmin
authors: X
authors: Chen
authors: Department of Enviromental Hygiene, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China.
authors: Si
authors: S
authors: Chen
authors: Surgical Department, Tangshan Gongren Hospital, Tangshan 063000, China.
authors: Yue
authors: Y
authors: Tan
authors: Department of Enviromental Hygiene, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China.
authors: Fei
authors: F
authors: Rong
authors: Department of Enviromental Hygiene, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China. Electronic address: jiangbozhu@aliyun.com.
authors: Jiangbo
authors: J
authors: Zhu
authors: Department of Enviromental Hygiene, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China. Electronic address: wenlingma@163.com.
authors: Wenling
authors: W
authors: Ma
mesh_terms: D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000627:therapeutic use*; D000818:Animals; D018491:Dopamine Agonists / Q000627:therapeutic use*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D000072233:Homer Scaffolding Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012892:Sleep Deprivation / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology
chemical_list: D018491:Dopamine Agonists; D000072233:Homer Scaffolding Proteins; C000606624:Homer1 protein, rat; D017447:Receptors, Dopamine D1; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: Chronic paradoxical sleep deprivation; D1 receptor; Gene chip; Prefrontal cortex; SKF38393; Signaling pathway