title: Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture.
abstract: Historically, two-dimensional (2D) cell culture has been the preferred method of producing disease models in vitro. Recently, there has been a move away from 2D culture in favor of generating three-dimensional (3D) multicellular structures, which are thought to be more representative of the in vivo environment. This transition has brought with it an influx of technologies capable of producing these structures in various ways. However, it is becoming evident that many of these technologies do not perform well in automated in vitro drug discovery units. We believe that this is a result of their incompatibility with high-throughput screening (HTS). In this study, we review a number of technologies, which are currently available for producing in vitro 3D disease models. We assess their amenability with high-content screening and HTS and highlight our own work in attempting to address many of the practical problems that are hampering the successful deployment of 3D cell systems in mainstream research.
authors: 1 Cancer and Ageing Research Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia .|2 Translational Cell Imaging Queensland, Institute of Health and Biomedical Innovation, Queensland University of Technology , Brisbane, Australia .
authors: Sarah-Louise
authors: SL
authors: Ryan
authors: 1 Cancer and Ageing Research Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia .|2 Translational Cell Imaging Queensland, Institute of Health and Biomedical Innovation, Queensland University of Technology , Brisbane, Australia .|3 Thoracic Oncology Research Group, Institute of Molecular Medicine , Trinity College Dublin, Dublin, Ireland .
authors: Anne-Marie
authors: AM
authors: Baird
authors: 4 Medical Chemistry Research Group, Institute of Molecular Medicine , Trinity College Dublin, Dublin, Ireland .
authors: Gisela
authors: G
authors: Vaz
authors: 1 Cancer and Ageing Research Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia .|2 Translational Cell Imaging Queensland, Institute of Health and Biomedical Innovation, Queensland University of Technology , Brisbane, Australia .
authors: Aaron J
authors: AJ
authors: Urquhart
authors: 4 Medical Chemistry Research Group, Institute of Molecular Medicine , Trinity College Dublin, Dublin, Ireland .
authors: Mathias
authors: M
authors: Senge
authors: 1 Cancer and Ageing Research Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia .
authors: Derek J
authors: DJ
authors: Richard
authors: 1 Cancer and Ageing Research Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia .|3 Thoracic Oncology Research Group, Institute of Molecular Medicine , Trinity College Dublin, Dublin, Ireland .|5 Division of Cancer Services, Princess Alexandra Hospital , Brisbane, Australia .
authors: Kenneth J
authors: KJ
authors: O'Byrne
authors: 2 Translational Cell Imaging Queensland, Institute of Health and Biomedical Innovation, Queensland University of Technology , Brisbane, Australia .|6 Irish National Centre for High Content Screening and Analysis, Institute of Molecular Medicine , Trinity College Dublin, Dublin, Ireland .
authors: Anthony M
authors: AM
authors: Davies
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods* / Q000706:statistics & numerical data; D055808:Drug Discovery / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006801:Humans