title: The expression of MMP-14 and microRNA-410 in FFPE tissues of human endometrial adenocarcinoma.
abstract: Endometrial cancer (EC) is the most common gynecological malignancy in Europe and North America. It is classified into two types exhibiting different characteristics and prognosis. Type I is an estrogen-dependent tumor, histologically classified as low grade and low stage, usually with an excellent prognosis. Type II EC is unrelated to estrogen stimulation and is characterized by a poor prognosis. MicroRNAs (miRNAs, miRs) are small non-coding RNA polynucleotides that regulate gene expression post-transcriptionally. Various dysregulations in microRNA expression are often considered to have an impact on the diagnosis, prognosis and overall survival in patients diagnosed with different types of cancers. Recent data suggest that microRNAs play an important role in the pathogenesis of EC. The aim of the study was to evaluate the involvement of matrix metaloprotease 14 (MMP-14) and microRNA-410 in formation of the EC tumor. To this end expression of MMP-14 and microRNA-410 was assessed within the cancer, transient and healthy zones in the histological sections of tumours using immunohistochemical staining and laser capture microdissection (LCM) followed by a quantitative real-time PCR. The results revealed significantly higher expression of MMP-14 in the cancer tissue zone in comparison to the healthy tissue zone, as well as a lower expression of microRNA-410 in the cancer zone compared with the healthy zone. This reverse correlation may suggest a regulatory role of miRNA-410 in modulating levels of MMP-14 in EC. This is the first report on such regulation in human endometrial cancer.
authors: Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw and Postgraduate School of Molecular Medicine, Warsaw, Poland.
authors: Beata
authors: B
authors: Rak
authors: Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.
authors: Filip
authors: F
authors: Garbicz
authors: Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.
authors: Wiktor
authors: W
authors: Paskal
authors: Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.
authors: Kacper
authors: K
authors: Pełka
authors: Department of Pathology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.
authors: Janina Maja
authors: JM
authors: Marczewska
authors: Department of Pathology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.
authors: Dominika
authors: D
authors: Wołosz
authors: Department of Histology and Embryology, Center for Biostructure Research, Medical University of Warsaw, Warsaw, Poland. pawel.wlodarski@wum.edu.pl.
authors: Paweł
authors: P
authors: Włodarski
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D061346:Laser Capture Microdissection; D053511:Matrix Metalloproteinase 14 / Q000096:biosynthesis*; D035683:MicroRNAs / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C568527:MIRN410 microRNA, human; D035683:MicroRNAs; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14