title: Molecular investigations of flaxseed mucilage polysaccharides.
abstract: The molecular properties of flaxseed mucilage were determined using a multi-angle laser light scattering (MALLS) detector coupled on-line to size exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4). Water and salt solution were tested as mobile phases. The SEC-MALLS method gave partial information and enabled molecular characterization of disaggregated mucilage molecules. Regardless of the eluent used, the observed Mw ranged from about 1.6 × 10(6) to more than 10 × 10(6) g/mol for mucilage polysaccharides. The AF4-MALLS system enabled a complete analysis of mucilage carbohydrate aggregates in water, in which two populations were satisfactorily separated. The molecular weight distribution (MWD) of molecules ranged from 1.5 × 10(6) to more than 4 × 10(8) g/mol. Experiments showed that the conformational structure of mucilage molecules was strongly influenced by ionic strength. Mucilage carbohydrates exhibited a spherical and compact structure in NaCl solution while they displayed a random-coil conformation in water.
authors: EA 3900, BIOPI, UFR des Sciences, UPJV, 33 Rue Saint Leu, F-80039 Amiens Cedex, France.
authors: Romain
authors: R
authors: Roulard
authors: EA 3900, BIOPI, UFR des Sciences, UPJV, 33 Rue Saint Leu, F-80039 Amiens Cedex, France.
authors: Emmanuel
authors: E
authors: Petit
authors: EA 3900, BIOPI, UFR des Sciences, UPJV, 33 Rue Saint Leu, F-80039 Amiens Cedex, France.
authors: François
authors: F
authors: Mesnard
authors: Institut Polytechnique LaSalle Beauvais, 19 rue Pierre Waguet, 60026 Beauvais, France. Electronic address: larbi.rhazi@lasalle-beauvais.fr.
authors: Larbi
authors: L
authors: Rhazi
mesh_terms: D002850:Chromatography, Gel; D019597:Flax / Q000737:chemistry*; D031521:Fractionation, Field Flow; D008027:Light; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012542:Scattering, Radiation; D012965:Sodium Chloride / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D014867:Water; D012965:Sodium Chloride
keywords: Flaxseed; Mucilage; Multi-angle light scattering