title: In Vivo Evaluation of Blood Based and Reference Tissue Based PET Quantifications of [11C]DASB in the Canine Brain.
abstract: This first-in-dog study evaluates the use of the PET-radioligand [11C]DASB to image the density and availability of the serotonin transporter (SERT) in the canine brain. Imaging the serotonergic system could improve diagnosis and therapy of multiple canine behavioural disorders. Furthermore, as many similarities are reported between several human neuropsychiatric conditions and naturally occurring canine behavioural disorders, making this tracer available for use in dogs also provide researchers an interesting non-primate animal model to investigate human disorders. Five adult beagles underwent a 90 minutes dynamic PET scan and arterial whole blood was sampled throughout the scan. For each ROI, the distribution volume (VT), obtained via the one- and two- tissue compartment model (1-TC, 2-TC) and the Logan Plot, was calculated and the goodness-of-fit was evaluated by the Akaike Information Criterion (AIC). For the preferred compartmental model BPND values were estimated and compared with those derived by four reference tissue models: 4-parameter RTM, SRTM2, MRTM2 and the Logan reference tissue model. The 2-TC model indicated in 61% of the ROIs a better fit compared to the 1-TC model. The Logan plot produced almost identical VT values and can be used as an alternative. Compared with the 2-TC model, all investigated reference tissue models showed high correlations but small underestimations of the BPND-parameter. The highest correlation was achieved with the Logan reference tissue model (Y = 0.9266 x + 0.0257; R2 = 0.9722). Therefore, this model can be put forward as a non-invasive standard model for future PET-experiments with [11C]DASB in dogs.
authors: Department of Radiopharmacy, Ghent University, Ghent, Belgium.
authors: Nick
authors: N
authors: Van Laeken
authors: Department of Medical Imaging and Small Animal Orthopedics, Ghent University, Ghent, Belgium.
authors: Olivia
authors: O
authors: Taylor
authors: Department of Medicine and Clinical Biology of Small Animals, Ghent University, Ghent, Belgium.
authors: Ingeborgh
authors: I
authors: Polis
authors: Department of Radiopharmacy, Ghent University, Ghent, Belgium.
authors: Sara
authors: S
authors: Neyt
authors: Department of Radiology and Nuclear Medicine, Ghent University Hospital, Ghent, Belgium.
authors: Ken
authors: K
authors: Kersemans
authors: Department of Radiology and Nuclear Medicine, Ghent University Hospital, Ghent, Belgium.
authors: Andre
authors: A
authors: Dobbeleir
authors: Department of Medical Imaging and Small Animal Orthopedics, Ghent University, Ghent, Belgium.
authors: Jimmy
authors: J
authors: Saunders
authors: Department of Radiology and Nuclear Medicine, Ghent University Hospital, Ghent, Belgium.
authors: Ingeborg
authors: I
authors: Goethals
authors: Department of Medical Imaging and Small Animal Orthopedics, Ghent University, Ghent, Belgium.
authors: Kathelijne
authors: K
authors: Peremans
authors: Department of Radiopharmacy, Ghent University, Ghent, Belgium.
authors: Filip
authors: F
authors: De Vos
mesh_terms: D000818:Animals; D001596:Benzylamines / Q000032:analysis* / Q000097:blood; D001923:Brain Chemistry; D004285:Dogs; D005260:Female; D008024:Ligands; D008297:Male; D059906:Neuroimaging / Q000662:veterinary; D049268:Positron-Emission Tomography / Q000662:veterinary*; D011869:Radioligand Assay / Q000662:veterinary; D012016:Reference Values; D050486:Serotonin Plasma Membrane Transport Proteins / Q000032:analysis / Q000097:blood; D014018:Tissue Distribution
chemical_list: D001596:Benzylamines; D008024:Ligands; C420885:N,N-dimethyl-2-(2-amino-4-cyanophenylthio)benzylamine; D050486:Serotonin Plasma Membrane Transport Proteins