title: Description of the Halophile Euplotes qatarensis nov. spec. (Ciliophora, Spirotrichea, Euplotida) Isolated from the Hypersaline Khor Al-Adaid Lagoon in Qatar.
abstract: The morphology, ontogenesis, and phylogenetic relationships of a halophile euplotid ciliates, Euplotes qatarensis nov. spec., isolated from the Khor Al-Adaid Lagoon in Qatar were investigated based on live observation as well as protargol- and silver nitrate-impregnated methods. The new species is characterised by a combination of features: the halophile habitat, a cell size of 50-65 × 33-40 μm, seven dorsal ridges, 10 commonly sized frontoventral cirri, two widely spaced marginal cirri, 10 dorsolateral kineties, and a double silverline pattern. The morphogenesis is similar to that of its congeners: (i) the oral primordium develops hypoapokinetally and the parental oral apparatus is retained; (ii) the frontoventral-transverse field of five streaks gives rise to the frontal, ventral, and transverse cirri, but not to the cirri I/1 and the marginal cirri; (iii) the dorsal somatic ciliature develops by intrakinetal proliferation of basal bodies in two anlagen per kinety that are just anterior and posterior to the future division furrow; (iv) the caudal cirri are formed by the two rightmost dorsolateral kineties. The SSU rDNA sequence of E. qatarensis branches with full support in the Euplotopsis elegans-Euplotes nobilii-Euplotopsis raikovi clade. The closest related publicly available SSU rDNA sequence is the one of E. nobilii, with which E. qatarensis has 93.4% sequence similarity. Euplotes parawoodruffi Song & Bradbury, 1997 is transferred to the genus Euplotoides based on the absence of frontoventral cirrus VI/3.
authors: Department of Genetic Engineering, Biotechnology Centre, Ministry of Environment, 7634 Doha, Qatar.
authors: Rashmi
authors: R
authors: Fotedar
authors: Department of Ecology, University of Kaiserslautern, D-67633, Kaiserslautern, Germany.
authors: Thorsten
authors: T
authors: Stoeck
authors: Department of Ecology, University of Kaiserslautern, D-67633, Kaiserslautern, Germany.
authors: Sabine
authors: S
authors: Filker
authors: Rosenstiel School of Marine and Atmospheric Science, University of Miami, Key Biscayne, FL, 33149, USA.
authors: Jack W
authors: JW
authors: Fell
authors: Department of Ecology and Evolution, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria.
authors: Sabine
authors: S
authors: Agatha
authors: Department of Genetic Engineering, Biotechnology Centre, Ministry of Environment, 7634 Doha, Qatar.
authors: Masoud
authors: M
authors: Al Marri
authors: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 201306, Shanghai, China.
authors: Jiamei
authors: J
authors: Jiang
mesh_terms: D000818:Animals; D001483:Base Sequence; D016798:Ciliophora / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D002965:Classification; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016812:Euplotes / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D016810:Hypotrichida / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*; D008853:Microscopy; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011780:Qatar; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D054712:Salinity; D012623:Seawater / Q000469:parasitology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D009626:Terminology as Topic
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
keywords: New species; SSU rDNA; ontogenesis; silverline pattern; taxonomy