title: Long-term heat stress induces the inflammatory response in dairy cows revealed by plasma proteome analysis.
abstract: In this work we employed a comparative proteomic approach to evaluate seasonal heat stress and investigate proteomic alterations in plasma of dairy cows. Twelve lactating Holstein dairy cows were used and the treatments were: heat stress (n = 6) in hot summer (at the beginning of the moderate heat stress) and no heat stress (n = 6) in spring natural ambient environment, respectively. Subsequently, heat stress treatment lasted 23 days (at the end of the moderate heat stress) to investigate the alterations of plasma proteins, which might be employed as long-term moderate heat stress response in dairy cows. Changes in plasma proteins were analyzed by two-dimensional electrophoresis (2-DE) combined with mass spectrometry. Analysis of the properties of the identified proteins revealed that the alterations of plasma proteins were related to inflammation in long-term moderate heat stress. Furthermore, the increase in plasma tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) directly demonstrated that long-term moderate heat stress caused an inflammatory response in dairy cows.
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China.
authors: Li
authors: L
authors: Min
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
authors: Nan
authors: N
authors: Zheng
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
authors: Shengguo
authors: S
authors: Zhao
authors: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
authors: Jianbo
authors: J
authors: Cheng
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
authors: Yongxin
authors: Y
authors: Yang
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
authors: Yangdong
authors: Y
authors: Zhang
authors: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China.
authors: Hongjian
authors: H
authors: Yang
authors: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: jiaqiwang@vip.163.com.
authors: Jiaqi
authors: J
authors: Wang
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood* / Q000276:immunology*; D002418:Cattle Diseases / Q000097:blood / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D005260:Female; D018882:Heat Stress Disorders / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D020543:Proteome / Q000276:immunology; D018746:Systemic Inflammatory Response Syndrome / Q000097:blood / Q000276:immunology / Q000662:veterinary*
chemical_list: D016207:Cytokines; D020543:Proteome
keywords: Dairy cows; Heat stress; Inflammatory response; Plasma proteome; Two-dimensional electrophoresis