title: Microbial colonization and lung function in adolescents with cystic fibrosis.
abstract: With intensified antibiotic therapy and longer survival, patients with cystic fibrosis (CF) are colonized with a more complex pattern of bacteria and fungi. However, the clinical relevance of these emerging pathogens for lung function remains poorly defined. The aim of this study was to assess the association of bacterial and fungal colonization patterns with lung function in adolescent patients with CF. Microbial colonization patterns and lung function parameters were assessed in 770 adolescent European (German/Austrian) CF patients in a retrospective study (median follow-up time: 10years). Colonization with Pseudomonas aeruginosa and MRSA were most strongly associated with loss of lung function, while mainly colonization with Haemophilus influenzae was associated with preserved lung function. Aspergillus fumigatus was the only species that was associated with an increased risk for infection with P. aeruginosa. Microbial interaction analysis revealed three distinct microbial clusters within the longitudinal course of CF lung disease. Collectively, this study identified potentially protective and harmful microbial colonization patterns in adolescent CF patients. Further studies in different patient cohorts are required to evaluate these microbial patterns and to assess their clinical relevance.
authors: Children's Hospital of the University of Tübingen, Tübingen, Germany.
authors: Andreas
authors: A
authors: Hector
authors: Children's Hospital of the University of Tübingen, Tübingen, Germany.
authors: Tobias
authors: T
authors: Kirn
authors: Children's Hospital of the University of Tübingen, Tübingen, Germany.
authors: Anjali
authors: A
authors: Ralhan
authors: Children's Hospital of the University of Tübingen, Tübingen, Germany.
authors: Ute
authors: U
authors: Graepler-Mainka
authors: Children's Hospital of the University of Tübingen, Tübingen, Germany.
authors: Sina
authors: S
authors: Berenbrinker
authors: Children's Hospital of the University of Tübingen, Tübingen, Germany.
authors: Joachim
authors: J
authors: Riethmueller
authors: Institute of Medical Microbiology and Hygiene, University of Frankfurt, Frankfurt, Germany.
authors: Michael
authors: M
authors: Hogardt
authors: Department of Pediatrics and Adolescence Medicine, Medical University of Graz, Graz, Austria.
authors: Marlies
authors: M
authors: Wagner
authors: Department of Pediatrics and Adolescence Medicine, Medical University of Graz, Graz, Austria.
authors: Andreas
authors: A
authors: Pfleger
authors: Institute of Medical Microbiology and Hygiene, University of Tübingen, Tübingen, Germany; German Center for Infection Research (DZIF), partner site Tübingen, Tübingen, Germany.
authors: Ingo
authors: I
authors: Autenrieth
authors: Department of Pediatric Pneumology, Hauner Children's Hospital, Ludwig-Maximilians University, Comprehensive Pneumology Center Munich (CPC-M), German Center for Lung Research (DZL), Munich, Germany.
authors: Matthias
authors: M
authors: Kappler
authors: Department of Pediatric Pneumology, Hauner Children's Hospital, Ludwig-Maximilians University, Comprehensive Pneumology Center Munich (CPC-M), German Center for Lung Research (DZL), Munich, Germany.
authors: Matthias
authors: M
authors: Griese
authors: Department of Pediatrics and Adolescence Medicine, Medical University of Graz, Graz, Austria.
authors: Ernst
authors: E
authors: Eber
authors: Institute of Clinical Epidemiology and Applied Biometry, University of Tübingen, Tübingen, Germany.
authors: Peter
authors: P
authors: Martus
authors: Children's Hospital of the University of Tübingen, Tübingen, Germany. Electronic address: dominik.hartl@med.uni-tuebingen.de.
authors: Dominik
authors: D
authors: Hartl
mesh_terms: D000293:Adolescent; D001232:Aspergillus fumigatus / Q000302:isolation & purification*; D001317:Austria / Q000453:epidemiology; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology; D005260:Female; D005858:Germany / Q000453:epidemiology; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000503:physiopathology; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D056265:Microbial Interactions; D011552:Pseudomonas Infections / Q000175:diagnosis / Q000453:epidemiology / Q000503:physiopathology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D055732:Pulmonary Aspergillosis / Q000175:diagnosis / Q000453:epidemiology / Q000503:physiopathology; D012129:Respiratory Function Tests / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology / Q000503:physiopathology; D013223:Statistics as Topic
keywords: Bacteria; Colonization; Cystic fibrosis; Fungi; Infection; Lung