title: Measurement of haem and total iron in fish, shrimp and prawn using ICP-MS: Implications for dietary iron intake calculations.
abstract: Twenty-five species of fish, shrimp and prawn from local markets in Bangladesh were analysed for concentrations of total Fe, haem Fe and non-haem Fe by ICP-MS. Total Fe and non-haem Fe concentrations were measured in nitric acid-digested samples and haem Fe was extracted using acidified 80% acetone for 60 min. Total Fe concentrations ranged from 0.55-14.43 mg/100 g FW, and haem Fe% ranged from 18%-93% of total Fe. Repeat extractions with 80% acetone recovered additional haem Fe, suggesting that previous measurement by this technique may have underestimated haem Fe content. Calculation of Fe balance (summing Fe in acetone extracts and Fe in the residue after haem Fe extraction) was not significantly different from total Fe, indicating the two processes recovered the different forms of Fe with similar effectiveness.
authors: Flinders University, School of Biological Sciences, GPO Box 2100, Adelaide, South Australia 5001, Australia. Electronic address: matthew.wheal@flinders.edu.au.
authors: Matthew S
authors: MS
authors: Wheal
authors: Flinders University, School of Biological Sciences, GPO Box 2100, Adelaide, South Australia 5001, Australia. Electronic address: emma.decourcyireland@flinders.edu.au.
authors: Emma
authors: E
authors: DeCourcy-Ireland
authors: WorldFish, Bangladesh, House 22 B, Road 7, BlockF, Banani, Dhaka 1213, Bangladesh; The University of Queensland, School of Public Health, Herston Road, Herston, Brisbane, Queensland 4006, Australia. Electronic address: j.bogard@worldfishcenter.org.
authors: Jessica R
authors: JR
authors: Bogard
authors: WorldFish, Bangladesh, House 22 B, Road 7, BlockF, Banani, Dhaka 1213, Bangladesh. Electronic address: s.thilsted@cgiar.org.
authors: Shakuntala H
authors: SH
authors: Thilsted
authors: Flinders University, School of Biological Sciences, GPO Box 2100, Adelaide, South Australia 5001, Australia. Electronic address: james.stangoulis@flinders.edu.au.
authors: James C R
authors: JC
authors: Stangoulis
mesh_terms: D000818:Animals; D003445:Crustacea; D005399:Fishes; D006418:Heme / Q000032:analysis*; D007501:Iron / Q000032:analysis*; D019266:Iron, Dietary / Q000008:administration & dosage*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D017747:Seafood / Q000032:analysis*
chemical_list: D019266:Iron, Dietary; D006418:Heme; D007501:Iron
keywords: Dietary intake; Fe protoporphyrin IX (PubChem Compound Database CID=53477764); Fish; Food analysis; Haem; Heme; Iron; Minerals