title: Efficient enzymatic production of hydroxy fatty acids by linoleic acid Δ9 hydratase from Lactobacillus plantarum AKU 1009a.
abstract: OBJECTIVE
  This study aims to produce hydroxy fatty acids efficiently.
  
  
  RESULTS
  Escherichia coli overexpressing linoleic acid Δ9 hydratase from Lactobacillus plantarum AKU 1009a was employed to produce hydroxy fatty acids with industrial potential. We found that 280 g l(-1) of linoleic acid (1 mol l(-1)) was converted into (S)-10-hydoxy-cis-12-octadecenoic acid (HYA) with a high conversion rate of 98% (mol/mol) and more than 99·9% enantiomeric excess (e.e.) by recombinant E. coli cells in the presence of FAD and NADH. In the same way, many kinds of C18 unsaturated fatty acids with Δ9 carbon double bond (280 g l(-1)) were converted into corresponding 10-hydroxy fatty acids with the conversion rates over 95% (mol/mol). We also produced HYA at a high rate of accumulation (289 g l(-1) ) with a high yield (97 mol%) in a reaction mixture that contained glucose instead of NADH.
  
  
  CONCLUSIONS
  We developed a process for producing several types of hydroxy fatty acids with high accumulation rates and high yields.
  
  
  CONCLUSIONS
  Hydroxy fatty acids are important materials for the chemical, food, cosmetic and pharmaceutical industries, and thus they have recently attracted much interest in a variety of research fields. However, the mass production of hydroxy fatty acids has been limited. This method of hydroxy fatty acids production will facilitate the widespread application of hydroxy fatty acids in various industries.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors: M
authors: M
authors: Takeuchi
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.|Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors: S
authors: S
authors: Kishino
authors: Laboratory of Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors: S-B
authors: SB
authors: Park
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors: A
authors: A
authors: Hirata
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors: N
authors: N
authors: Kitamura
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
authors: A
authors: A
authors: Saika
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.|Research Unit for Physiological Chemistry, Kyoto University, Kyoto, Japan.
authors: J
authors: J
authors: Ogawa
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D006880:Hydroxy Acids / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000235:genetics; D019787:Linoleic Acid / Q000378:metabolism; D030781:Organisms, Genetically Modified / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D006880:Hydroxy Acids; D019787:Linoleic Acid
keywords: C18 unsaturated fatty acids; hydration; hydroxy fatty acid; lactic acid bacteria; linoleic acid; linoleic acid Δ9 hydratase; production