title: Upregulation of metastasis-associated PRL-3 initiates chordoma in zebrafish.
abstract: The metastasis-associated phosphatase of regenerating liver-3 (PRL-3) plays multiple roles in progression of various human cancers; however, significance of its role during development has not been addressed. Here we cloned and characterized the expression pattern of zebrafish prl-3 transcript and showed that it is ubiquitiously expressed in the first 24 h of development with both maternal and zygotic expressions. The transcripts become progressively restricted to the notochord, vessels and the intestine by 96 h post-fertilization. Notably, overexpression of zebrafish Prl-3 (zPrl-3) and human PRL-3 induces notochord malformation in zebrafish. This phenotype resembles chordoma and is confirmed by associated misexpression of notochord-specific markers. Clinical significance of the PRL-3 in chordoma is strongly suggested by detection of PRL-3 antigen in clinical chordoma specimens. Collectively, our results uncovered that aberrant overexpression of PRL-3 could initiate chordoma in early development and suggest the use of PRL-3 could be used as a predictor and a therapeutic target for chordoma.
authors: Translational Medicine Centre, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Li
authors: L
authors: Li
authors: Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Hongshun
authors: H
authors: Shi
authors: Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Mingming
authors: M
authors: Zhang
authors: Translational Medicine Centre, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Xiaoling
authors: X
authors: Guo
authors: Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Fang
authors: F
authors: Tong
authors: Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Wenliang
authors: W
authors: Zhang
authors: Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Junyi
authors: J
authors: Zhou
authors: Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Haihe
authors: H
authors: Wang
authors: Translational Medicine Centre, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong CPZN 510080, P.R. China.
authors: Shulan
authors: S
authors: Yang
mesh_terms: D000818:Animals; D002817:Chordoma / Q000235:genetics* / Q000473:pathology; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution; D015854:Up-Regulation; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D009363:Neoplasm Proteins; D029961:Zebrafish Proteins; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases; C000613079:ptp4a3a protein, zebrafish