title: Production of Butyrate from Lactate by a Newly Isolated Clostridium sp. BPY5.
abstract: Lactate-utilizing bacteria play important roles in the production of Chinese strong-flavored liquor (CSFL). However, the identity of these bacteria and their lactate-utilizing properties are largely unknown. Here, a lactate-utilizing, butyrate-producing bacterium BPY5 was isolated from an old fermentation pit for CSFL production. The isolate represented a novel species belonging to Clostridium cluster XIVa of family Lachnospiraceae based on phylogenetic analysis using 16S rRNA gene sequences. Strain BPY5 could ferment lactate into butyrate as the major metabolic product. Butyrate was significantly formed at initial lactate concentration from 66 to 104 mM, but substantially declined when initial lactate exceeded 133 mM. At initial lactate concentration of 66 mM, lactate conversion was independent on initial pH from 5.5 to 7.0, but the conversion was completely inhibited when pH dropped below 4.8. Nevertheless, the inhibition on lactate conversion was largely relieved by the addition of acetate, suggesting that exogenous acetate could enhance lactate conversion at low pH condition. Additionally, lactate in CSFL-brewing wastewater was dramatically removed when inoculated with strain BPY5. These results implicate that the isolate may be applied for the industrial production of butyrate or the recovery of butyrate from lactate-containing wastewater.
authors: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu, 610041, China. taoyong@cib.ac.cn.
authors: Yong
authors: Y
authors: Tao
authors: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu, 610041, China.
authors: Xiaohong
authors: X
authors: Hu
authors: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu, 610041, China.
authors: Xiaoyu
authors: X
authors: Zhu
authors: Chengdu Medical College, Chengdu, 610083, China.
authors: Hong
authors: H
authors: Jin
authors: The National-Recognized Enterprise Technology Center, Sichuan Jiannanchun Group Co., Ltd., Mianzhu, Sichuan, 618200, People's Republic of China.
authors: Zhancheng
authors: Z
authors: Xu
authors: The National-Recognized Enterprise Technology Center, Sichuan Jiannanchun Group Co., Ltd., Mianzhu, Sichuan, 618200, People's Republic of China.
authors: Qinglan
authors: Q
authors: Tang
authors: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu, 610041, China. Lixz@cib.ac.cn.
authors: Xiangzhen
authors: X
authors: Li
mesh_terms: D000085:Acetates / Q000737:chemistry; D000434:Alcoholic Beverages / Q000382:microbiology; D020148:Butyric Acid / Q000737:chemistry / Q000378:metabolism*; D003013:Clostridium / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000085:Acetates; D012336:RNA, Ribosomal, 16S; D020148:Butyric Acid; D019344:Lactic Acid
keywords: Butyrate production; CSFL brewing fermentation; Clostridium cluster XIVa; Lactate conversion; Wastewater