title: Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy.
abstract: The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells' before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.
authors: Quantitative Biology Center (QBiC), RIKEN.
authors: Ahmed
authors: A
authors: Ali
authors: Yasmine
authors: Y
authors: Abouleila
authors: Sara
authors: S
authors: Amer
authors: Rie
authors: R
authors: Furushima
authors: Samy
authors: S
authors: Emara
authors: Sebastien
authors: S
authors: Equis
authors: Yann
authors: Y
authors: Cotte
authors: Tsutomu
authors: T
authors: Masujima
mesh_terms: D003600:Cytosol / Q000378:metabolism; D056945:Hep G2 Cells; D006696:Holography / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D012031:Refractometry; D059010:Single-Cell Analysis / Q000379:methods*; D014054:Tomography / Q000379:methods*