title: Data in support of FSH induction of IRS-2 in human granulosa cells: Mapping the transcription factor binding sites in human IRS-2 promoter.
abstract: Insulin receptor substrate-2 (IRS-2) plays critical role in the regulation of various metabolic processes by insulin and IGF-1. The defects in its expression and/or function are linked to diseases like polycystic ovary syndrome (PCOS), insulin resistance and cancer. To predict the transcription factors (TFs) responsible for the regulation of human IRS-2 gene expression, the transcription factor binding sites (TFBS) and the corresponding TFs were investigated by analysis of IRS-2 promoter sequence using MatInspector Genomatix software (Cartharius et al., 2005 [1]). The ibid data is part of author׳s publication (Anjali et al., 2015 [2]) that explains Follicle stimulating hormone (FSH) mediated IRS-2 promoter activation in human granulosa cells and its importance in the pathophysiology of PCOS. Further analysis was carried out for binary interactions of TF regulatory genes in IRS-2 network using Cytoscape software tool and R-code. In this manuscript, we describe the methodology used for the identification of TFBSs in human IRS-2 promoter region and provide details on experimental procedures, analysis method, validation of data and also the raw files. The purpose of this article is to provide the data on all TFBSs in the promoter region of human IRS-2 gene as it has the potential for prediction of the regulation of IRS-2 gene in normal or diseased cells from patients with metabolic disorders and cancer.
authors: Division of Molecular Endocrinology and Reproduction, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Surleen
authors: S
authors: Kaur
authors: Division of Molecular Endocrinology and Reproduction, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: G
authors: G
authors: Anjali
authors: Division of Molecular Endocrinology and Reproduction, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Priya
authors: P
authors: Bhardwaj
authors: Division of Molecular Endocrinology and Reproduction, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Jyoti
authors: J
authors: Taneja
authors: Division of Molecular Endocrinology and Reproduction, Department of Zoology, University of Delhi, Delhi 110007, India.
authors: Rita
authors: R
authors: Singh
keywords: ChIP; FSH; IRS-2; SP1; TFBS