title: The microRNA-dependent cell fate of multipotent stromal cells differentiating to endothelial cells.
abstract: In the endothelial recovery process, bone marrow-derived MSCs are a potential source of cells for both research and therapy, and their capacities to self-renew and to differentiate into all the cell types in the human body make them a promising therapeutic agent for remodeling cellular differentiation and a valuable resource for the treatment of many diseases. Based on the results provided in a miRNA database, we selected miRNAs with unique targets in cell fate-related signaling pathways. The tested miRNAs targeting GSK-3β (miR-26a), platelet-derived growth factor receptor, and CD133 (miR-26a and miR-29b) induced MSC differentiation into functional ECs, whereas miRNAs targeting VEGF receptor (miR-15, miR-144, miR-145, and miR-329) inhibited MSC differentiation into ECs through VEGF stimulation. In addition, the expression levels of these miRNAs were correlated with in vivo physiological endothelial recovery processes. These findings indicate that the miRNA expression profile is distinct for cells in different stages of differentiation from MSCs to ECs and that specific miRNAs can function as regulators of endothelialization.
authors: Institute for Integrative Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea; Comprehensive Care Hospital for Cancer Patients, Catholic Kwandong University International St. Mary's Hospital, Incheon 22711, Republic of Korea; Catholic Kwandong University International St. Mary's Hospital, Incheon 22711, Republic of Korea.
authors: Min-Ji
authors: MJ
authors: Cha
authors: Catholic Kwandong University International St. Mary's Hospital, Incheon 22711, Republic of Korea; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea.
authors: Eunhyun
authors: E
authors: Choi
authors: Catholic Kwandong University International St. Mary's Hospital, Incheon 22711, Republic of Korea; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea.
authors: Seahyoung
authors: S
authors: Lee
authors: Institute for Integrative Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea; Catholic Kwandong University International St. Mary's Hospital, Incheon 22711, Republic of Korea.
authors: Byeong-Wook
authors: BW
authors: Song
authors: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea; Department of Cardiovascular & Thoracic Surgery, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea.
authors: Cheesoon
authors: C
authors: Yoon
authors: Catholic Kwandong University International St. Mary's Hospital, Incheon 22711, Republic of Korea; Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Republic of Korea. Electronic address: kchwang@cku.ac.kr.
authors: Ki-Chul
authors: KC
authors: Hwang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000235:genetics* / Q000502:physiology; D019070:Cell Lineage; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D035683:MicroRNAs / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D035683:MicroRNAs
keywords: Endothelial cells; MicroRNAs; Multipotent stromal cells; Stem cell fate