title: LIN7A is a major determinant of cell-polarity defects in breast carcinomas.
abstract: BACKGROUND
  Polarity defects are a hallmark of most carcinomas. Cells from invasive micropapillary carcinomas (IMPCs) of the breast are characterized by a striking cell polarity inversion and represent an interesting model for the analysis of polarity abnormalities.
  
  
  METHODS
  In-depth investigation of polarity proteins in 24 IMPCs and a gene expression profiling, comparing IMPC (n = 73) with invasive carcinomas of no special type (ICNST) (n = 51) have been performed.
  
  
  RESULTS
  IMPCs showed a profound disorganization of the investigated polarity proteins and revealed major abnormalities in their subcellular localization. Gene expression profiling experiments highlighted a number of deregulated genes in the IMPCs that have a role in apico-basal polarity, adhesion and migration. LIN7A, a Crumbs-complex polarity gene, was one of the most differentially over-expressed genes in the IMPCs. Upon LIN7A over-expression, we observed hyperproliferation, invasion and a complete absence of lumen formation, revealing strong polarity defects.
  
  
  CONCLUSIONS
  This study therefore shows that LIN7A has a crucial role in the polarity abnormalities associated with breast carcinogenesis.
authors.affiliation: Institut Curie, PSL Research University, INSERM U830, 26 rue d'Ulm, 75248, Paris cédex 05, France. Nadege.gruel@curie.fr.|Département de Recherche Translationnelle, Institut Curie, PSL Research University, 26 rue d'Ulm, 75248, Paris cédex 05, France. Nadege.gruel@curie.fr.
authors.forename: Nadège
authors.initials: N
authors.lastname: Gruel
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, 26 rue d'Ulm, 75248, Paris cédex 05, France. Laetitia.fuhrmann@curie.fr.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Fuhrmann
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, 26 rue d'Ulm, 75248, Paris cédex 05, France. Catalina.lodillinsky@curie.fr.
authors.forename: Catalina
authors.initials: C
authors.lastname: Lodillinsky
authors.affiliation: Institut Curie, PSL Research University, INSERM U830, 26 rue d'Ulm, 75248, Paris cédex 05, France. Vanessa.benhamo@curie.fr.|Département de Recherche Translationnelle, Institut Curie, PSL Research University, 26 rue d'Ulm, 75248, Paris cédex 05, France. Vanessa.benhamo@curie.fr.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Benhamo
authors.affiliation: Department of Pathology, Institut Curie, 26 rue d'Ulm, 75248, Paris cédex 05, France. Odette.mariani@curie.fr.
authors.forename: Odette
authors.initials: O
authors.lastname: Mariani
authors.affiliation: Department of Pathology, Institut Curie, 26 rue d'Ulm, 75248, Paris cédex 05, France. Aurelie.cedenot@curie.fr.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Cédenot
authors.affiliation: Département de Pathologie and Centre de Ressources Biologiques Ferdinand Cabanne, Centre Georges François Leclerc, 1 rue Professeur Marion, BP 77980, 21079, Dijon cédex, France. LArnould@cgfl.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Arnould
authors.affiliation: Institut Bergonié, Service de Biopathologie, 229 cours de l'Argonne, 33076, Bordeaux, France. G.MacGrogan@bordeaux.unicancer.fr.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Macgrogan
authors.affiliation: Department of Pathology, Institut Curie, 26 rue d'Ulm, 75248, Paris cédex 05, France. Xavier.sastre@curie.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Sastre-Garau
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, 26 rue d'Ulm, 75248, Paris cédex 05, France. Philippe.chavrier@curie.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Chavrier
authors.affiliation: Institut Curie, PSL Research University, INSERM U830, 26 rue d'Ulm, 75248, Paris cédex 05, France. Olivier.delattre@curie.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Delattre
authors.affiliation: Institut Curie, PSL Research University, INSERM U830, 26 rue d'Ulm, 75248, Paris cédex 05, France. Anne.salomon@curie.fr.|Department of Pathology, Institut Curie, 26 rue d'Ulm, 75248, Paris cédex 05, France. Anne.salomon@curie.fr.
authors.forename: Anne
authors.initials: A
authors.lastname: Vincent-Salomon
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D016764:Cell Polarity / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D033921:Vesicular Transport Proteins
chemical_list: C486532:LIN7A protein, human; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins