title: Electroactive biodegradable polyurethane significantly enhanced Schwann cells myelin gene expression and neurotrophin secretion for peripheral nerve tissue engineering.
abstract: Myelination of Schwann cells (SCs) is critical for the success of peripheral nerve regeneration, and biomaterials that can promote SCs' neurotrophin secretion as scaffolds are beneficial for nerve repair. Here we present a biomaterials-approach, specifically, a highly tunable conductive biodegradable flexible polyurethane by polycondensation of poly(glycerol sebacate) and aniline pentamer, to significantly enhance SCs' myelin gene expression and neurotrophin secretion for peripheral nerve tissue engineering. SCs are cultured on these conductive polymer films, and the biocompatibility of these films and their ability to enhance myelin gene expressions and sustained neurotrophin secretion are successfully demonstrated. The mechanism of SCs' neurotrophin secretion on conductive films is demonstrated by investigating the relationship between intracellular Ca(2+) level and SCs' myelination. Furthermore, the neurite growth and elongation of PC12 cells are induced by adding the neurotrophin medium suspension produced from SCs-laden conductive films. These data suggest that these conductive degradable polyurethanes that enhance SCs' myelin gene expressions and sustained neurotrophin secretion perform great potential for nerve regeneration applications.
authors.affiliation: Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Yaobin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China. Electronic address: baoling@mail.xjtu.edu.cn.
authors.forename: Baolin
authors.initials: B
authors.lastname: Guo
authors.affiliation: Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
authors.forename: Yongpin
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Biologic and Materials Sciences, University of Michigan, 1011, North University Ave., Room 2209, Ann Arbor, MI, 48109, USA; Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, MI, 48109, USA; Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: mapx@umich.edu.
authors.forename: Peter X
authors.initials: PX
authors.lastname: Ma
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D004553:Electric Conductivity; D009186:Myelin Sheath / Q000187:drug effects* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009416:Nerve Regeneration / Q000187:drug effects; D016716:PC12 Cells; D010525:Peripheral Nerves / Q000187:drug effects / Q000502:physiology; D011140:Polyurethanes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D023822:Tissue Engineering; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D009414:Nerve Growth Factors; D011140:Polyurethanes
keywords: Biomaterials-approach; Conductive polyurethane; Myelin gene expressions; Neurotrophins secretion; Peripheral nerve tissue engineering; Schwann cells