title: Submicron-surface structured tricalcium phosphate ceramic enhances the bone regeneration in canine spine environment.
abstract: Calcium phosphate ceramics with submicron-scaled surface structure can trigger bone formation in non-osseous sites and are expected to enhance bone formation in spine environment. In this study, two tricalcium phosphate ceramics having either a submicron-scaled surface structure (TCP-S) or a micron-scaled one (TCP-B) were prepared and characterized regarding their physicochemical properties. Granules (size 1-2 mm) of both materials were implanted on either left or right side of spinous process, between the two lumbar vertebrae (L3-L4), and in paraspinal muscle of eight beagles. After 12 weeks of implantation, ectopic bone was observed in muscle in TCP-S explants (7.7 ± 3.7%), confirming their ability to inductively form bone in non-osseous sites. In contrast, TCP-B implants did not lead to bone formation in muscle. Abundant bone (34.1 ± 6.6%) was formed within TCP-S implants beside the two spinous processes, while limited bone (5.1 ± 4.5%) was seen in TCP-B. Furthermore, the material resorption of TCP-S was more pronounced than that of TCP-B in both the muscle and spine environments. The results herein indicate that the submicron-scaled surface structured tricalcium phosphate ceramic could enhance bone regeneration as compared to the micron-scaled one in spine environment. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1865-1873, 2016.
authors.affiliation: Biomaterial Science and Technology, MIRA Institute, University of Twente, Enschede, The Netherlands.|Xpand Biotechnology BV, Bilthoven, The Netherlands.|Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
authors.forename: Rongquan
authors.identifier: 0000-0003-0728-8337
authors.initials: R
authors.lastname: Duan
authors.affiliation: Xpand Biotechnology BV, Bilthoven, The Netherlands.
authors.forename: Davide
authors.initials: D
authors.lastname: Barbieri
authors.affiliation: Biomaterial Science and Technology, MIRA Institute, University of Twente, Enschede, The Netherlands.|Xpand Biotechnology BV, Bilthoven, The Netherlands.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Luo
authors.affiliation: Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Weng
authors.affiliation: Biomaterial Science and Technology, MIRA Institute, University of Twente, Enschede, The Netherlands.|Xpand Biotechnology BV, Bilthoven, The Netherlands.|Department of Materials Science, Queen Marry University of London, London, United Kingdom.
authors.forename: Joost D
authors.initials: JD
authors.lastname: de Bruijn
authors.affiliation: Xpand Biotechnology BV, Bilthoven, The Netherlands.|College of Physical Science and Technology, Sichuan University, Chengdu, China.|Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, Maastricht, 6229 ER, The Netherlands.
authors.forename: Huipin
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000494:pharmacology / Q000627:therapeutic use*; D002516:Ceramics / Q000737:chemistry*; D004285:Dogs; D008297:Male; D019736:Prostheses and Implants; D013122:Spinal Diseases / Q000628:therapy*
chemical_list: D002130:Calcium Phosphates; C018392:tricalcium phosphate
keywords: Spine; bone regeneration; bone substitutes; calcium phosphate ceramics; submicron-scaled surface structure