title: Structural, thermal, functional, antioxidant & antimicrobial properties of β-d-glucan extracted from baker's yeast (Saccharomyces cereviseae)-Effect of γ-irradiation.
abstract: This study was carried out to evaluate the effect of γ-irradiation (0, 5, 10, 20, 30 & 50kGy) on the structural, functional, antioxidant and antimicrobial properties of yeast β-d-glucan. The samples were characterized by ATR-FTIR, gel permeation chromatography (GPC) and the thermal properties were studied using DSC. There was a decrease in the average molecular weight of β-d-glucan as the irradiation dose increased. The functional properties of irradiated yeast β-d-glucan were largely influenced by the action of gamma radiation like swelling power and viscosity decreases with increase in the irradiation dose while as fat binding capacity, emulsifying properties, foaming properties and bile acid binding capacity shows an increasing trend. All the antioxidant properties carried out using six different assays increased significantly (p≤0.05) in a dose dependent manner. The antibacterial activity of yeast β-d-glucan also showed an increasing trend with increase in the irradiation dose from 5 to 50kDa.
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 19006, India.
authors.forename: Asma Ashraf
authors.initials: AA
authors.lastname: Khan
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 19006, India. Electronic address: adil.gani@gmail.com.
authors.forename: Adil
authors.initials: A
authors.lastname: Gani
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 19006, India.
authors.forename: F A
authors.initials: FA
authors.lastname: Masoodi
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 19006, India.
authors.forename: Furheen
authors.initials: F
authors.lastname: Amin
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 19006, India.
authors.forename: Idrees Ahmed
authors.initials: IA
authors.lastname: Wani
authors.affiliation: Department of Biotechnology, University of Kashmir, Hazratbal, Srinagar 19006, India.
authors.forename: Firdous Ahmad
authors.initials: FA
authors.lastname: Khanday
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand.
authors.forename: Asir
authors.initials: A
authors.lastname: Gani
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001647:Bile Acids and Salts / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D004249:DNA Damage; D005720:Gamma Rays; D007501:Iron / Q000737:chemistry; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013696:Temperature; D014783:Viscosity; D047071:beta-Glucans / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001647:Bile Acids and Salts; D001713:Biphenyl Compounds; D007502:Iron Chelating Agents; D010851:Picrates; D047071:beta-Glucans; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron
keywords: Antimicrobial; Antioxidant; Degradation; Irradiation; Structural analysis; β-d-Glucan