title: Estimation of Antifungal Activity of Mevastatin Produced by Aspergillus terreus GCBL-03 on pretreated substrate in solid state fermentation.
abstract: This project was planned to study the production of cholesterol lowering drug mevastatin by Aspergillus terreus GCBL-03 on pretreated substrate bagasse in solid state fermentation. Bagasse was pretreated by potassium hydroxide to delignify the substrate to readily become available to microorganism. It was noticed that pretreated bagasse exhibited 13.69±0.64 mg/100mL yield of mevastatin and 6.63±0.48 g dry cell mass as compared to nontreated substrate that showed 7.04±0.81 mg/100 mL and 6.04±0.24 g dry cell mass. The fermentation profile like pH (5.5), temperature (30 °C), moisture contents (60%) inoculum size (2 mL), and incubation time (72 h) showed the optimum production (30.63±1.24 mg/100 mL) of mevastatin. Furthermore, fermented drug showed noteworthy antifungal activity against battery of selected fungal strains assessed by disc diffusion and measurement of minimum inhibitory concentration by micro dilution method. It was concluded from the study that fermented mevastatin was effective against fungal strains.
authors.affiliation: Department of Applied Chemistry & Biochemistry, Government College University, Faisalabad, Pakistan. drsadiajaved@yahoo.com.
authors.forename: Sadia
authors.initials: S
authors.lastname: Javed
authors.affiliation: Department of Applied Chemistry & Biochemistry, Government College University, Faisalabad, Pakistan. bukhari.shazia@yahoo.com.
authors.forename: Shazia A
authors.initials: SA
authors.lastname: Bukhari
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ali
authors.lastname: Sajjad-ur-Rehman
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D001230:Aspergillus / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D015394:Molecular Structure
chemical_list: D000935:Antifungal Agents; C012258:mevastatin; D002482:Cellulose; C027433:bagasse; D008148:Lovastatin