title: Hyperglycemia-Induced Hypovolemia Is Involved in Early Cardiac Magnetic Resonance Alterations in Streptozotocin-Induced Diabetic Mice: A Comparison with Furosemide-Induced Hypovolemia.
abstract: OBJECTIVE
  The aim of the study was to assess the early features of diabetic cardiomyopathy using cardiac magnetic resonance within the first week after streptozotocin injection in mice. We focused on the relationship between left ventricular function and hypovolemia markers in diabetic animals compared to a hypovolemic rodent model.
  
  
  RESULTS
  Swiss mice were randomized into control (group C), streptozotocin-induced diabetes (group D) and furosemide-induced hypovolemia (group F) groups. Cardiac magnetic resonance, non-invasive blood pressure, urine volume, plasma markers of dehydration and cardiac histology were assessed in all groups. Mean blood glucose was higher in diabetic animals than in groups C and F (30.5±5.8 compared to 10.4±2.1 and 11.1±2.8 mmol/L, respectively; p<0.01). Diuresis was increased in animals from group D and F compared to C (14650±11499 and 1533±540 compared to 192±111 μL/24 h; p<0.05). End diastolic and end systolic volumes were lower in group D than in group C at week 1 (1.52±0.36 vs. 1.93±0.35 and 0.54±0.22 vs. 0.75±0.18 mL/kg, p<0.05). These left ventricular volume values in group D were comparable to those observed in the acute hypovolemia model (group F). Increased dehydration plasma markers and an absence of obvious intrinsic myocardial damage (evaluated by cardiac magnetic resonance and histology) suggest that a hemodynamic mechanism underlies the very early drop in left ventricular volumes in group D and provides a potential link to hyperglycemic osmotic diuresis.
  
  
  CONCLUSIONS
  Researchers using cardiac magnetic resonance in hyperglycemic rodent models should be aware of this hemodynamic mechanism, which may partially explain modifications in cardiac parameters in addition to diabetic myocardial damage.
authors.affiliation: Diabetes care unit, Caen University Hospital, Caen, France.|EA4650 Université Caen Normandie, GIP Cyceron, Caen, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Joubert
authors.affiliation: Nuclear Medicine department, Caen University Hospital, Caen, France.|EA4650 Université Caen Normandie, GIP Cyceron, Caen, France.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Bellevre
authors.affiliation: Cardiology unit, Caen University Hospital, Caen, France.|EA4650 Université Caen Normandie, GIP Cyceron, Caen, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Legallois
authors.affiliation: CMABIO-HIQ facility, SF4206 ICORE, IBFA, Université Caen Normandie, Caen, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Elie
authors.affiliation: Biochemical unit, Caen University Hospital, Caen, France.|EA4650 Université Caen Normandie, GIP Cyceron, Caen, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Coulbault
authors.affiliation: Biochemical unit, Caen University Hospital, Caen, France.|EA4650 Université Caen Normandie, GIP Cyceron, Caen, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Allouche
authors.affiliation: Nuclear Medicine department, Caen University Hospital, Caen, France.|EA4650 Université Caen Normandie, GIP Cyceron, Caen, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Manrique
mesh_terms: D000818:Animals; D057791:Cardiac Imaging Techniques; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000503:physiopathology*; D005665:Furosemide; D006943:Hyperglycemia / Q000150:complications* / Q000473:pathology / Q000503:physiopathology*; D020896:Hypovolemia / Q000139:chemically induced* / Q000150:complications* / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice
chemical_list: D005665:Furosemide