title: microRNA-363 plays a tumor suppressive role in osteosarcoma by directly targeting MAP2K4.
abstract: Abnormal expression of microRNAs plays important functions in osteosarcoma. The aim of this study was to investigate expression, functions and molecular mechanisms of microRNA-363 in osteosarcoma. Quantitative Real-time PCR was used to detect the expression level of microRNA-363 in osteosarcoma tissue samples and cell lines. After transfection, CCK8 assay, cell migration and invasion assay, western blot and Dual-Luciferase report assay were performed in human osteosarcoma cells. According to the results, we found that microRNA-363 was down-regulated in osteosarcoma tissues and cell lines. In addition, low expression level of microRNA-363 was associated with tumor size, clinical stage and distant metastasis. Moreover, microRNA-363 targeted MAP2K4 to inhibit osteosarcoma cell growth, migration and invasion. In conclusion, microRNA-363 played a tumor suppressive role in osteosarcoma by directly targeting MAP2K4. These findings indicated that microRNA-363 may have therapeutic value in treating osteosarcoma.
authors.affiliation: Department of Orthopaedic Surgery, Yidu Central Hospital of Weifang, Weifang Medical University Qingzhou 262500, Shandong, China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, Yidu Central Hospital of Weifang, Weifang Medical University Qingzhou 262500, Shandong, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, Yidu Central Hospital of Weifang, Weifang Medical University Qingzhou 262500, Shandong, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Orthopaedic Surgery, Yidu Central Hospital of Weifang, Weifang Medical University Qingzhou 262500, Shandong, China.
authors.forename: Huazhuang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, Yidu Central Hospital of Weifang, Weifang Medical University Qingzhou 262500, Shandong, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Chen
keywords: MAP2K4; growth; metastasis; microRNA-363; osteosarcoma