title: Stichopus japonicus Polysaccharide, Fucoidan, or Heparin Enhanced the SDF-1α/CXCR4 Axis and Promoted NSC Migration via Activation of the PI3K/Akt/FOXO3a Signaling Pathway.
abstract: Stichopus japonicus Polysaccharide (SJP) is a sulfated polysaccharide from the body wall of the sea cucumber, Stichopus japonicus. Fucoidan is a heparinoid compound that belongs to a family of sulfated polyfucose polysaccharides. Heparin is a glycosaminoglycan. SJP, fucoidan, and heparin profoundly promoted stromal cell-derived factor 1 alpha (SDF-1α)-induced neural stem cell (NSC) migration in a concentration-dependent manner. In addition, the basal migration capacity of cells was significantly promoted after incubation with SJP, fucoidan, or heparin. Interaction of SJP, fucoidan, or heparin with SDF-1α efficiently showed additive effects on the promotion of cell migration from the neurosphere. SJP, fucoidan, or heparin interaction with SDF-1α treatment could increase Nestin expression. SDF-1α modulated by SJP, fucoidan, or heparin activated the CXCR4 receptor and directed cellular migration via the activation of the PI3K/Akt/FOXO3a signaling pathway. Moreover, interaction of SJP, fucoidan, or heparin with SDF-1α effectively promoted NSC migration and induced SDF-1α and CXCR4 expressions. Results suggested that SJP, fucoidan, and heparin might be good candidates for alleviating injury-initiated signals to which NSCs respond.
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.|School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Cui
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Ningshan
authors.initials: N
authors.lastname: Cui
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Shuliang
authors.initials: S
authors.lastname: Song
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: Marine College, Shandong University, Weihai, Shandong, China. jiaiguo@sdu.edu.cn.|School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, China. jiaiguo@sdu.edu.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Ji
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000378:metabolism*; D000071316:Forkhead Box Protein O3 / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D019718:Receptors, CXCR4 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D047528:Stichopus / Q000737:chemistry
chemical_list: D054377:Chemokine CXCL12; D000071316:Forkhead Box Protein O3; D006493:Heparin; D019869:Phosphatidylinositol 3-Kinases; D011134:Polysaccharides; D019718:Receptors, CXCR4; C575935:CXCL12 protein, rat; C486264:Cxcr4 protein, rat; C499515:FOXO3 protein, rat; C007789:fucoidan
keywords: CXCR4; Fucoidan; Heparin; NSC; SDF-1α; SJP