title: Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity.
abstract: Resveratrol has been reported to inhibit monoamine oxidases (MAO). Many substrates or inhibitors of neuronal MAO interact also with other amine oxidases (AO) in peripheral organs, such as semicarbazide-sensitive AO (SSAO), known as primary amine oxidase, absent in neurones, but abundant in adipocytes. We asked whether phenolic compounds (resveratrol, pterostilbene, quercetin, and caffeic acid) behave as MAO and SSAO inhibitors. AO activity was determined in human adipose tissue. Computational docking and glucose uptake assays were performed in 3D models of human AO proteins and in adipocytes, respectively. Phenolic compounds fully inhibited the fluorescent detection of H2O2 generated during MAO and SSAO activation by tyramine and benzylamine. They also quenched H2O2-induced fluorescence in absence of biological material and were unable to abolish the oxidation of radiolabelled tyramine and benzylamine. Thus, phenolic compounds hampered H2O2 detection but did not block AO activity. Only resveratrol and quercetin partially impaired MAO-dependent [(14)C]-tyramine oxidation and behaved as MAO inhibitors. Phenolic compounds counteracted the H2O2-dependent benzylamine-stimulated glucose transport. This indicates that various phenolic compounds block downstream effects of H2O2 produced by biogenic or exogenous amine oxidation without directly inhibiting AO. Phenolic compounds remain of interest regarding their capacity to limit oxidative stress rather than inhibiting AO.
authors.affiliation: Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale (INSERM U1048), I2MC, 31432 Toulouse Cedex 4, France; Université Paul Sabatier, I2MC-UPS, CHU Rangueil, 31432 Toulouse Cedex 4, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Carpéné
authors.affiliation: Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale (INSERM U1048), I2MC, 31432 Toulouse Cedex 4, France; Université Paul Sabatier, I2MC-UPS, CHU Rangueil, 31432 Toulouse Cedex 4, France.
authors.forename: Mounia
authors.initials: M
authors.lastname: Hasnaoui
authors.affiliation: Department of Organic Chemistry, Semmelweiss University, Hőgyes Endre Utca 7, Budapest 1095, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Balogh
authors.affiliation: Department of Organic Chemistry, Semmelweiss University, Hőgyes Endre Utca 7, Budapest 1095, Hungary.
authors.forename: Peter
authors.initials: P
authors.lastname: Matyus
authors.affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Centre, 01006 Vitoria, Spain; CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 28029 Madrid, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fernández-Quintela
authors.affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Centre, 01006 Vitoria, Spain; CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 28029 Madrid, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Rodríguez
authors.affiliation: Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale (INSERM U1048), I2MC, 31432 Toulouse Cedex 4, France.
authors.forename: Josep
authors.initials: J
authors.lastname: Mercader
authors.affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Centre, 01006 Vitoria, Spain; CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 28029 Madrid, Spain.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry; D000893:Anti-Inflammatory Agents / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry; D001596:Benzylamines / Q000737:chemistry; D002109:Caffeic Acids / Q000737:chemistry; D004032:Diet; D005260:Female; D005470:Fluorometry; D006601:Hexoses / Q000493:pharmacokinetics; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007249:Inflammation; D008875:Middle Aged; D062105:Molecular Docking Simulation; D008995:Monoamine Oxidase / Q000737:chemistry*; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry*; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry; D014439:Tyramine / Q000737:chemistry; D055815:Young Adult
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001596:Benzylamines; D002109:Caffeic Acids; D006601:Hexoses; D008996:Monoamine Oxidase Inhibitors; D010636:Phenols; D013267:Stilbenes; C107773:pterostilbene; D011794:Quercetin; C030796:benzylamine; D006861:Hydrogen Peroxide; D006631:Amine Oxidase (Copper-Containing); D008995:Monoamine Oxidase; D000077185:Resveratrol; D010100:Oxygen; C040048:caffeic acid; D014439:Tyramine