title: MiR-98 inhibits cell proliferation and invasion of non-small cell carcinoma lung cancer by targeting PAK1.
abstract: A family of small non-coding RNAs, ~22 nt in length, known as microRNAs (miRNAs), regulating ~30% of all human gene expression, have been reported to be involved in the pathogenesis of a number of types of cancers, including non-small cell carcinoma lung cancer (NSCLC). P21-activated protein kinase 1 (PAK1) is a clinical biomarker of Non-small carcinoma lung cancer. Here, we found that miR-98 is down-regulated, whereas PAK1 is highly expressed in NSCLC tissues and cells. We demonstrated that miR-98 directly targets the 3'UTR of PAK1 and down-regulates its expression at the mRNA and protein level. Also, miR-98 inhibited and PAK1 stimulated proliferation, migration, invasion and apoptosis of NSCLC cells. In agreement, PAK1 over-expression counteracted the inhibitory effect of miR-98. This current study suggests that exogenous miR-98 may serve as novel potential maker for NSCLC therapy.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Jining Medical University Jining City, Shandong Province, 272000, P.R. China.
authors.forename: Guangxia
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Jining Medical University Jining City, Shandong Province, 272000, P.R. China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Critical Care Medicine, Jining NO. 1 People's Hospital Jining City, Shandong Province, 272011, P.R. China.
authors.forename: Jikui
authors.initials: J
authors.lastname: Shi
keywords: PAK1; miR-98; miRNA; non-small cell carcinoma lung cancer