title: Hexanoyl-Chitosan-PEG Copolymer Coated Iron Oxide Nanoparticles for Hydrophobic Drug Delivery.
abstract: Nanoparticle (NP) formulations may be used to improvein vivoefficacy of hydrophobic drugs by circumventing solubility issues and providing targeted delivery. In this study, we developed a hexanoyl-chitosan-PEG (CP6C) copolymer coated, paclitaxel (PTX)-loaded, and chlorotoxin (CTX) conjugated iron oxide NP (CTX-PTX-NP) for targeted delivery of PTX to human glioblastoma (GBM) cells. We modified chitosan with polyethylene glycol (PEG) and hexanoyl groups to obtain the amphiphilic CP6C. The resultant copolymer was then coated onto oleic acid-stabilized iron oxide NPs (OA-IONP)viahydrophobic interactions. PTX, a model hydrophobic drug, was loaded into the hydrophobic region of IONPs. CTX-PTX-NP showed high drug loading efficiency (>30%), slow drug release in PBS and the CTX-conjugated NP was shown to successfully target GBM cells. Importantly, the NPs showed great therapeutic efficacy when evaluated in GBM cell line U-118 MG. Our results indicate that this nanoparticle platform could be used for loading and targeted delivery of hydrophobic drugs.
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195, USA; Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu City 300, Taiwan.
authors.forename: Meng-Hsuan
authors.initials: MH
authors.lastname: Hsiao
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Mu
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Zachary R
authors.initials: ZR
authors.lastname: Stephen
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195, USA; Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Fang
authors.affiliation: Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Miqin
authors.initials: M
authors.lastname: Zhang