title: Role of a Structurally Equivalent Phenylalanine Residue in Catalysis and Thermal Stability of Formate Dehydrogenases from Different Sources.
abstract: Comparison of amino acid sequences of NAD+-dependent formate dehydrogenases (FDH, EC 1.2.1.2) from different sources and analysis of structures of holo-forms of FDH from bacterium Pseudomonas sp. 101 (PseFDH) and soya Glycine max (SoyFDH) as well as of structure of apo-form of FDH from yeast Candida boidinii (CboFDH) revealed the presence on the surface of protein globule of hydrophobic Phe residue in structurally equivalent position (SEP). The residue is placed in the coenzyme-binding domain and protects bound NAD+ from solvent. The effects of amino acid changes of the SEP on catalytic properties and thermal stability of PseFDH, SoyFDH, and CboFDH were compared. The strongest effect was observed for SoyFDH. All eight amino acid replacements resulted in increase in thermal stability, and in seven cases, increase in catalytic constant was achieved. Thermal stability of SoyFDH after mutations Phe290Asp and Phe290Glu increased 66- and 55-fold, respectively. KM values of the enzyme for substrate and coenzyme in different cases slightly increased or decreased. In case of one CboFDH, the mutein catalytic constant increased and thermal stability did not changed. In case of the second CboFDH mutant, results were the opposite. In one PseFDH mutant, amino acid change did not influence the catalytic constant, but in three others, the parameter was reduced. Two PseFDH mutants had higher and two mutants lower thermal stability in comparison with initial enzyme. Analysis of results of SEP mutagenesis in FDHs from bacterium, yeast, and plant shows that protein structure plays a key role for effect of the same amino acid changes in equivalent position in protein globule of formate dehydrogenases from different sources.
authors.affiliation: Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119991, Russia. vitishkov@gmail.com.
authors.forename: V I
authors.initials: VI
authors.lastname: Tishkov
authors.forename: K V
authors.initials: KV
authors.lastname: Goncharenko
authors.forename: A A
authors.initials: AA
authors.lastname: Alekseeva
authors.forename: S Yu
authors.initials: SY
authors.lastname: Kleymenov
authors.forename: S S
authors.initials: SS
authors.lastname: Savin
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology; D055162:Biocatalysis; D004795:Enzyme Stability; D056890:Eukaryota / Q000201:enzymology; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010649:Phenylalanine; D017434:Protein Structure, Tertiary
chemical_list: D009243:NAD; D010649:Phenylalanine; D005560:Formate Dehydrogenases