title: Fluorescent Peptoids as Selective Live Cell Imaging Probes.
abstract: This paper describes the synthesis of fluorescent peptoids using the Ugi multicomponent reaction (4CR). The four synthesized structures had their photophysical properties evaluated and their potential as biomarkers established. The peptidomimetics were used at very low concentrations (10 nM) to follow their internalization in breast cancer cells and had their localization precisely determined. One of the new peptoids displayed mitochondrial affinity and stained this important organelle selectively. Co-staining experiments using MitoTracker Red confirmed the localization inside live cells.
authors.affiliation: Laboratory of Bioactive Compounds Synthesis N.T.S., University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, Brasília, DF, Brazil.
authors.forename: Saulo T A
authors.initials: ST
authors.lastname: Passos
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasília, Chemistry Institute (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP 70904-970, P.O. Box 4478, Brasília, DF, Brazil.
authors.forename: José R
authors.initials: JR
authors.lastname: Correa
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasília, Chemistry Institute (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP 70904-970, P.O. Box 4478, Brasília, DF, Brazil.
authors.forename: Samira L M
authors.initials: SL
authors.lastname: Soares
authors.affiliation: Laboratory of Bioactive Compounds Synthesis N.T.S., University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, Brasília, DF, Brazil.
authors.forename: Wender A
authors.initials: WA
authors.lastname: da Silva
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasília, Chemistry Institute (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP 70904-970, P.O. Box 4478, Brasília, DF, Brazil.
authors.forename: Brenno A D
authors.initials: BA
authors.lastname: Neto
mesh_terms: D014408:Biomarkers, Tumor / Q000737:chemistry*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry; D057786:Peptidomimetics / Q000737:chemistry*; D034444:Peptoids / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D057786:Peptidomimetics; D034444:Peptoids