title: Dominant Tree Species and Soil Type Affect the Fungal Community Structure in a Boreal Peatland Forest.
abstract: Boreal peatlands play a crucial role in global carbon cycling, acting as an important carbon reservoir. However, little information is available on how peatland microbial communities are influenced by natural variability or human-induced disturbances. In this study, we have investigated the fungal diversity and community structure of both the organic soil layer and buried wood in boreal forest soils using high-throughput sequencing of the internal transcribed spacer (ITS) region. We have also compared the fungal communities during the primary colonization of wood with those of the surrounding soils. A permutational multivariate analysis of variance (PERMANOVA) confirmed that the community composition significantly differed between soil types (P< 0.001) and tree species (P< 0.001). The distance-based linear models analysis showed that environmental variables were significantly correlated with community structure (P< 0.04). The availability of soil nutrients (Ca [P= 0.002], Fe [P= 0.003], and P [P= 0.003]) within the site was an important factor in the fungal community composition. The species richness in wood was significantly lower than in the corresponding soil (P< 0.004). The results of the molecular identification were supplemented by fruiting body surveys. Seven of the genera of Agaricomycotina identified in our surveys were among the top 20 genera observed in pyrosequencing data. Our study is the first, to our knowledge, fungal high-throughput next-generation sequencing study performed on peatlands; it further provides a baseline for the investigation of the dynamics of the fungal community in the boreal peatlands.
authors.affiliation: Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China Hui.Sun@helsinki.fi.|Department of Forest Sciences, University of Helsinki, Helsinki, Finland.|Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Forest Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Eeva
authors.initials: E
authors.lastname: Terhonen
authors.affiliation: Department of Forest Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Andriy
authors.initials: A
authors.lastname: Kovalchuk
authors.affiliation: Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Tuovila
authors.affiliation: Department of Forest Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Forest Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Abbot O
authors.initials: AO
authors.lastname: Oghenekaro
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Jussi
authors.initials: J
authors.lastname: Heinonsalo
authors.affiliation: UMR 1136 INRA/Université de Lorraine, Interactions Arbres/Microorganismes, INRA, Institut National de la Recherche Agronomique, Centre INRA de Nancy, Champenoux, France.
authors.forename: Annegret
authors.initials: A
authors.lastname: Kohler
authors.affiliation: Department of Forest Sciences, University of Helsinki, Helsinki, Finland.|Natural Resources Institute Finland (LUKE), Vantaa, Finland.
authors.forename: Risto
authors.initials: R
authors.lastname: Kasanen
authors.affiliation: Department of Forest Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Harri
authors.initials: H
authors.lastname: Vasander
authors.affiliation: Department of Forest Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Fred O
authors.initials: FO
authors.lastname: Asiegbu
mesh_terms: D044822:Biodiversity; D017753:Ecosystem; D005387:Finland; D065928:Forests; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D014197:Trees / Q000382:microbiology*; D014934:Wood / Q000382:microbiology
chemical_list: D012987:Soil