title: TRPV1 channel as a target for cancer therapy using CNT-based drug delivery systems.
abstract: Carbon nanotubes are being considered for the design of drug delivery systems (DDSs) due to their capacity to internalize molecules and control their release. However, for cellular uptake of drugs, this approach requires an active translocation pathway or a channel to transport the drug into the cell. To address this issue, it is suggested to use TRPV1 ion channels as a potential target for drug release by nano-DDSs since these channels are overexpressed in cancer cells and allow the permeation of large cationic molecules. Considering these facts, this work presents three studies using molecular dynamics simulations of a human TRPV1 (hTRPV1) channel built here. The purpose of these simulations is to study the interaction between a single-wall carbon nanotube (SWCNT) and hTRPV1, and the diffusion of doxorubicin (DOX) across hTRPV1 and across a POPC lipid membrane. The first study shows an attractive potential between the SWCNT surface and hTRPV1, tilting the adsorbed SWCNT. The second study shows low diffusion probability of DOX across the open hTRPV1 due to a high free energy barrier. Although, the potential energy between DOX and hTRPV1 reveals an attractive interaction while DOX is inside hTRPV1. These results suggest that if the channel is dilated, then DOX diffusion could occur. The third study shows a lower free energy barrier for DOX across the lipid membrane than for DOX across hTRPV1. Taking into account the results obtained, it is feasible to design novel nano-DDSs based on SWCNTs to accomplish controlled drug release into cells using as translocation pathway, the hTRPV1 ion channel.
authors.affiliation: School of Electrical and Electronics Engineering, Bionanoelectronics Research Group, Universidad del Valle, Cali, Colombia.
authors.forename: Andres
authors.initials: A
authors.lastname: Ortega-Guerrero
authors.affiliation: Department of Chemistry, Center for Theoretical and Computational Nanoscience, Indiana University, Bloomington, IN, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Espinosa-Duran
authors.affiliation: School of Electrical and Electronics Engineering, Bionanoelectronics Research Group, Universidad del Valle, Cali, Colombia. jaime.velasco@correounivalle.edu.co.
authors.forename: Jaime
authors.initials: J
authors.lastname: Velasco-Medina
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004317:Doxorubicin / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D058990:Molecular Targeted Therapy; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010539:Permeability; D010713:Phosphatidylcholines / Q000378:metabolism; D011487:Protein Conformation; D051381:Rats; D050916:TRPV Cation Channels / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008051:Lipid Bilayers; D037742:Nanotubes, Carbon; D010713:Phosphatidylcholines; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D004317:Doxorubicin; C028694:1-palmitoyl-2-oleoylphosphatidylcholine
keywords: Carbon nanotubes; Doxorubicin; Drug delivery system; Molecular dynamics; TRPV1 channel