title: Real-time PCR melting analysis with fiber optic SPR enables multiplex DNA identification of bacteria.
abstract: A fiber optic surface plasmon resonance (FO-SPR) technology was developed that enables simultaneous quantification and identification of multiple DNA targets on the same platform. The bioassay was based on the hybridization/melting of DNA-coated Au nanoparticles on the FO-SPR sensor when targets are present. The multiplex concept was successfully demonstrated on two related bacteria and for detection of multiple mutations in sequences. In conclusion, FO-SPR technology shows a great potential as a next generation in vitro diagnostics tool.
authors.affiliation: KU Leuven - University of Leuven, BIOSYST-MeBioS, Willem de Croylaan 42, B-3001 Leuven, Belgium. Jeroen.lammertyn@biw.kuleuven.be.
authors.forename: D
authors.initials: D
authors.lastname: Daems
authors.affiliation: KU Leuven - University of Leuven, BIOSYST-MeBioS, Willem de Croylaan 42, B-3001 Leuven, Belgium. Jeroen.lammertyn@biw.kuleuven.be.
authors.forename: K
authors.initials: K
authors.lastname: Knez
authors.affiliation: KU Leuven - University of Leuven, BIOSYST-MeBioS, Willem de Croylaan 42, B-3001 Leuven, Belgium. Jeroen.lammertyn@biw.kuleuven.be.
authors.forename: F
authors.initials: F
authors.lastname: Delport
authors.affiliation: KU Leuven - University of Leuven, BIOSYST-MeBioS, Willem de Croylaan 42, B-3001 Leuven, Belgium. Jeroen.lammertyn@biw.kuleuven.be.
authors.forename: D
authors.initials: D
authors.lastname: Spasic
authors.affiliation: KU Leuven - University of Leuven, BIOSYST-MeBioS, Willem de Croylaan 42, B-3001 Leuven, Belgium. Jeroen.lammertyn@biw.kuleuven.be.
authors.forename: J
authors.initials: J
authors.lastname: Lammertyn
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D009691:Nucleic Acid Denaturation; D055100:Optical Fibers; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D044366:Transition Temperature
chemical_list: D004269:DNA, Bacterial