title: An ultra-high pressure liquid chromatography-tandem mass spectrometry method for the quantification of teicoplanin in plasma of neonates.
abstract: The development and validation of an ultra-high pressure liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) method was performed with the aim to be applied for the quantification of plasma teicoplanin concentrations in neonates. Pharmacokinetic data of teicoplanin in the neonatal population is very limited, therefore, a sensitive and reliable method for the determination of all isoforms of teicoplanin applied in a low volume of sample is of real importance. Teicoplanin main components were extracted by a simple acetonitrile precipitation step and analysed on a C18 chromatographic column by a triple quadrupole MS with electrospray ionization. The method provides quantitative data over a linear range of 25-6400ng/mL with LOD 8.5ng/mL and LOQ 25ng/mL for total teicoplanin. The method was applied in plasma samples from neonates to support pharmacokinetic data and proved to be a reliable and fast method for the quantification of teicoplanin concentration levels in plasma of infants during therapy in Intensive Care Unit.
authors.affiliation: Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: O
authors.initials: O
authors.lastname: Begou
authors.affiliation: 1st Department of Neonatology, Aristotle University of Thessaloniki, Hippokration Hospital, 54642 Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Kontou
authors.affiliation: Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Raikos
authors.affiliation: 1st Department of Neonatology, Aristotle University of Thessaloniki, Hippokration Hospital, 54642 Thessaloniki, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Sarafidis
authors.affiliation: Infectious Diseases Unit, 3rd Department of Pediatrics, Aristotle University of Thessaloniki, Hippokration Hospital, 54642 Thessaloniki, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Roilides
authors.affiliation: Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: I N
authors.initials: IN
authors.lastname: Papadoyannis
authors.affiliation: Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Electronic address: gkikae@auth.gr.
authors.forename: H G
authors.initials: HG
authors.lastname: Gika
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D057230:Limit of Detection; D053719:Tandem Mass Spectrometry / Q000379:methods*; D017334:Teicoplanin / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D017334:Teicoplanin
keywords: Antibiotic determination; Liquid chromatography–mass spectrometry; Plasma; Teicoplanin