title: Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety.
abstract: Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour.
authors.affiliation: National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea. jy0820@korea.kr.
authors.forename: Jong-Yeol
authors.initials: JY
authors.lastname: Lee
authors.affiliation: National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea.
authors.forename: Hye-Rang
authors.initials: HR
authors.lastname: Beom
authors.affiliation: USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA, 94710, USA.
authors.forename: Susan B
authors.initials: SB
authors.lastname: Altenbach
authors.affiliation: National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea.
authors.forename: Sun-Hyung
authors.initials: SH
authors.lastname: Lim
authors.affiliation: Department of Bioresource Sciences, Andong National University, Andong, 760-749, Korea.
authors.forename: Yeong-Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: National Institute of Crop Science, RDA, Jeonju, 565-851, Korea.
authors.forename: Chon-Sik
authors.initials: CS
authors.lastname: Kang
authors.affiliation: National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea.
authors.forename: Ung-Han
authors.initials: UH
authors.lastname: Yoon
authors.affiliation: Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Korea.
authors.forename: Sun-Tae
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Department of Agronomy, Chungnam National University, Daejeon, 357-764, Korea.
authors.forename: Sang-Nag
authors.initials: SN
authors.lastname: Ahn
authors.affiliation: National Academy of Agricultural Science, RDA, Jeonju, 560-500, Korea. ymikim@korea.kr.
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Kim
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence / Q000235:genetics*; D001939:Bread; D015180:Electrophoresis, Gel, Two-Dimensional; D005983:Glutens / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D008970:Molecular Weight; D011544:Pseudogenes / Q000235:genetics; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000235:genetics*
chemical_list: D005983:Glutens; C037302:glutenin
keywords: LMW-GS genes; Proteomics; Triticum aestivum L.; Wheat quality improvement