title: Study on the Alkaloids in Tibetan Medicine Aconitum pendulum Busch by HPLC-MSn Combined with Column Chromatography.
abstract: A rapid, convenient and effective identification method of alkaloids was established and an attempt on isolating and analyzing the alkaloids in Aconitum pendulum Busch was conducted successfully. In this article, four high-content components including deoxyaconitine, benzoylaconine, aconine and neoline were isolated by using column chromatography. HPLC-MS(n)was employed to deduce the regulations of fragmentation of diterpenoid alkaloids which displayed a characteristic behavior of loss of CO(28u), CH3COOH(60u), CH3OH(32u), H2O(18u) and C6H5COOH(122u). Then, according to fragmentation regulation of mass spectrometry, 42 alkaloids were found inA. pendulum Among them, 38 compounds were identified and 29 alkaloids were reported for the first time for this herb. Therefore, this means that HPLC-MS(n)combined with column chromatography could work as an effective and reliable tool for rapid identification of the chemical components of herbal medicine.
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Wang
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Dong
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Ji
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Jiarui
authors.initials: J
authors.lastname: Wu
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Tan
authors.affiliation: Beijing University of Chinese Medicine, Beijing 100102, China liuyg0228@163.com.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D038861:Medicine, Tibetan Traditional; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids