title: The genome of the Antarctic polyextremophile Nesterenkonia sp. AN1 reveals adaptive strategies for survival under multiple stress conditions.
abstract: Nesterenkonia sp. AN1 is a polyextremophile isolated from Antarctic desert soil. Genomic analyses and genome comparisons with three mesophilic Nesterenkonia strains indicated that the unique genome fraction of Nesterenkonia sp. AN1 contains adaptive features implicated in the response to cold stress including modulation of membrane fluidity as well as response to cold-associated osmotic and oxidative stress. The core genome also encodes a number of putative cold stress response proteins. RNA-Seq-based transcriptome analyses of Nesterenkonia sp. AN1 grown at 5ºC and 21°C showed that there was significant induction of transcripts that code for antioxidants at 5ºC, demonstrated by the upregulation of sodA, bcp and bpoA2. There was also overexpression of universal stress protein genes related to uspA, along with genes encoding other characterized cold stress features. Genes encoding the two key enzymes of the glyoxylate cycle, isocitrate lyase (ICL) and malate synthase (AceB) were induced at 5ºC, suggesting possible adaptation strategies for energy metabolism in cold habitats. These genomic features may contribute to the survival of Nesterenkonia sp. AN1 in arid Antarctic soils.
authors.affiliation: Centre for Microbial Ecology and Genomics, University of Pretoria, Pretoria 0028, South Africa Department of Genetics, University of Pretoria, Pretoria 0028, South Africa.
authors.forename: Habibu
authors.initials: H
authors.lastname: Aliyu
authors.affiliation: Centre for Microbial Ecology and Genomics, University of Pretoria, Pretoria 0028, South Africa Department of Microbiology, University of Pretoria, Pretoria 0028, South Africa.
authors.forename: Pieter
authors.initials: P
authors.lastname: De Maayer
authors.affiliation: Centre for Microbial Ecology and Genomics, University of Pretoria, Pretoria 0028, South Africa Department of Genetics, University of Pretoria, Pretoria 0028, South Africa don.cowan@up.ac.za.
authors.forename: Don
authors.initials: D
authors.lastname: Cowan
mesh_terms: D000864:Antarctic Regions; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D057773:Cold Shock Proteins and Peptides / Q000235:genetics*; D058639:Cold-Shock Response / Q000235:genetics*; D004734:Energy Metabolism / Q000235:genetics* / Q000502:physiology; D020869:Gene Expression Profiling; D016680:Genome, Bacterial / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D007522:Isocitrate Lyase / Q000096:biosynthesis / Q000235:genetics; D008292:Malate Synthase / Q000096:biosynthesis / Q000235:genetics; D008835:Micrococcaceae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009997:Osmotic Pressure / Q000502:physiology; D018384:Oxidative Stress / Q000235:genetics*; D012988:Soil Microbiology; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D057773:Cold Shock Proteins and Peptides; D006360:Heat-Shock Proteins; C078564:universal stress protein A, Bacteria; C068491:bacterioferritin comigratory protein, Bacteria; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase; D008292:Malate Synthase; D007522:Isocitrate Lyase
keywords: Antarctic soil; Nesterenkonia; genomics; polyextremophile; psychrotolerant; transcriptomics