title: Ultraviolet-A triggers photoaging in model nematode Caenorhabditis elegans in a DAF-16 dependent pathway.
abstract: Ultraviolet radiations (UV) are the primary causative agent for skin aging (photoaging) and cancer, especially UV-A. The mode of action and the molecular mechanism behind the damages caused by UV-A is not well studied, in vivo. The current study was employed to investigate the impact of UV-A exposure using the model organism, Caenorhabditis elegans. Analysis of lifespan, healthspan, and other cognitive behaviors were done which was supported by the molecular mechanism. UV-A exposure on collagen damages the synthesis and functioning which has been monitored kinetically using engineered strain, col-19:: GFP. The study results suggested that UV-A accelerated the aging process in an insulin-like signaling pathway dependent manner. Mutant (daf-2)-based analysis concrete the observations of the current study. The UV-A exposure affected the usual behavior of the worms like pharyngeal movements and brood size. Quantitative PCR profile of the candidate genes during UV-A exposure suggested that continuous exposure has damaged the neural network of the worms, but the mitochondrial signaling and dietary restriction pathway remain unaffected. Western blot analysis of HSF-1 evidenced the alteration in protein homeostasis in UV-A exposed worms. Outcome of the current study supports our view that C. elegans can be used as a model to study photoaging, and the mode of action of UV-A-mediated damages can be elucidated which will pave the way for drug developments against photoaging.
authors.affiliation: Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 004, India.
authors.forename: Mani Iyer
authors.initials: MI
authors.lastname: Prasanth
authors.affiliation: Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 004, India.
authors.forename: Gunasekaran Santhi
authors.initials: GS
authors.lastname: Santoshram
authors.affiliation: ITC - Life Sciences and Technology Centre, ITC Limited, No. 3, 1st Main, Peenya Industrial Area, Bangalore, Karnataka, 560058, India.
authors.forename: James Prabhanand
authors.initials: JP
authors.lastname: Bhaskar
authors.affiliation: Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, -630 004, India. bsuryar@yahoo.com.
authors.forename: Krishnaswamy
authors.initials: K
authors.lastname: Balamurugan
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000235:genetics*; D051858:Forkhead Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects*; D008136:Longevity / Q000235:genetics* / Q000528:radiation effects; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics*; D015398:Signal Transduction / Q000528:radiation effects; D015595:Skin Aging / Q000235:genetics / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; C108831:daf-16 protein, C elegans; D012313:RNA
keywords: C. elegans; IIS pathway; Lifespan; Photoaging; UV-A