title: Effects of multipurpose solutions on the adhesion of Acanthamoeba to rigid gas permeable contact lenses.
abstract: OBJECTIVE
  To evaluate the effect of multipurpose contact lens care solutions (MPSs) on the adhesion of Acanthamoeba to rigid gas permeable (RGP) contact lenses.
  
  
  METHODS
  Acanthamoeba castellanii (AC) trophozoites were inoculated onto untreated RGP contact lenses (FP, Extra, or Menicon Z), and numbers of trophozoites adhering to lenses were counted under a phase contrast microscope at 18 h post-inoculation (controls). Similarly, adhering trophozoites were counted at 6 h post-inoculation on each of the three RGP lens types with one of three MPSs (Boston Simplus, Menicare Plus, and O2 Care). Scanning electron microscopic examinations were performed to compare lens surfaces.
  
  
  RESULTS
  Adhesion of AC trophozoites to untreated FP was greater than to untreated Extra or Menicon Z. Surfaces of Extra and Menicon Z lenses were waxier, smoother, and more homogeneous than those of FP lenses. After treatment with Boston Simplus, adhesion of AC trophozoites was significantly reduced for all lens types as compared with controls (p < 0.0001). Treatments with Menicare Plus or O2 Care reduced the number of adherent AC trophozoites significantly on FP lenses only as compared with controls (p < 0.0001).
  
  
  CONCLUSIONS
  The adhesion rates of AC trophozoites to RGP lenses depended on lens surfaces. Boston Simplus reduced the adhesion rate of AC trophozoites more than Menicare Plus or O2 Care. Appropriate RGP lens and MPS selection could decrease the prevalence of Acanthamoeba keratitis.
authors.affiliation: Department of Cellular and Molecular Biology, California State University, Chico, CA, USA.
authors.forename: Ga-Hyun
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Department of Parasitology, Pusan National University College of Medicine, Yangsan, Korea.
authors.forename: Hak-Sun
authors.initials: HS
authors.lastname: Yu
authors.affiliation: Department of Ophthalmology, Pusan National University College of Medicine, Yangsan, Korea.|Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Lee
mesh_terms: D048248:Acanthamoeba castellanii / Q000187:drug effects*; D000704:Analysis of Variance; D001422:Bacterial Adhesion / Q000187:drug effects*; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003261:Contact Lenses / Q000382:microbiology*; D004202:Disinfectants / Q000494:pharmacology*; D006801:Humans; D008854:Microscopy, Electron
chemical_list: D018639:Contact Lens Solutions; D004202:Disinfectants
keywords: Acanthamoeba castellanii; chlorhexidine; multipurpose solution; rigid gas permeable contact lens