title: Elevation of AQP4 and selective cytokines in experimental autoimmune encephalitis mice provides some potential biomarkers in optic neuritis and demyelinating diseases.
abstract: Idiopathic optic neuritis (ION) is an inflammation of the optic nerve that may result in a complete or partial loss of vision. ION is usually due to the immune attack of the myelin sheath covering the optic nerve. ION acts frequently as the first symptoms of multiple sclerosis (MS) and neuromyelitis optica (NMO), or other inflammatory demyelinating disorders. The pathogenic progression of ION remains unclear. Experimental autoimmune encephalitis (EAE) is a commonly used model of idiopathic inflammatory demyelinating disorders (IIDDs); the optic nerve is affected in EAE as well. The specific mediators of demyelination in optic neuritis are unknown. Recent studies have indicated what T-cell activation in peripheral blood is associated with optic neuritis pathogenesis. The object of the present study was to determine whether certain cytokines (IL-6, IL-17A, and IL-23) and AQP4 contribute to the demyelinating process using EAE model. We have found that IL-6R, AQP4 and IL-23R are significantly increased in mRNA and protein levels in optic nerves in EAE mice compared to control mice; serum AQP4, IL-6, IL-17A, IL-23 are increased whereas transforming growth factor beta (TGF-β) is decreased in EAE mice. These results suggest that AQP4 and selective cytokines in serum are associated with ION pathogenesis in the animal model, and these results shine light for future clinical diagnosis as potential biomarkers in ION patients.
authors.affiliation: Department of Ophthalmology, Huashan Hospital, Fudan University Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Ophthalmology, Huashan Hospital, Fudan University Shanghai, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Weng
authors.affiliation: Department of Neurology, Huashan Hospital, Fudan University Shanghai, China.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D003711:Demyelinating Diseases / Q000097:blood / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000097:blood / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D005074:Evoked Potentials, Visual; D005260:Female; D020381:Interleukin-17 / Q000378:metabolism; D053759:Interleukin-23 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008810:Mice, Inbred C57BL; D009900:Optic Nerve / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009902:Optic Neuritis / Q000097:blood / Q000175:diagnosis / Q000378:metabolism* / Q000503:physiopathology; D011237:Predictive Value of Tests; D011379:Prognosis; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C496031:Aqp4 protein, mouse; D051401:Aquaporin 4; D015415:Biomarkers; D016207:Cytokines; D020381:Interleukin-17; D053759:Interleukin-23; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; C577686:interleukin-6, mouse
keywords: AQP4; cytokines; experimental autoimmune encephalitis; idiopathic optic neuritis