title: Structure-activity correlation in transfection promoted by pyridinium cationic lipids.
abstract: The efficiency of the transfection of a plasmid DNA encoding a galactosidase promoted by a series of pyridinium lipids in mixtures with other cationic lipids and neutral lipids was assessed in CHO-K1 cells. We identify key molecular parameters of the lipids in the mixture - clog P, lipid length, partial molar volume - to predict the morphology of the lipid-DNA lipoplex and then correlate these same parameters with transfection efficiency in an in vitro assay. We define a Transfection Index that provides a linear correlation with normalized transfection efficiency over a series of 90 different lipoplex compositions. We also explore the influence of the same set of molecular parameters on the cytotoxicity of the formulations.
authors.affiliation: Department of Chemistry, University of Victoria, P.O. Box 3065, Stn CSC, Victoria, BC V8W 3V6, Canada. tmf@uvic.ca.
authors.forename: P
authors.initials: P
authors.lastname: Parvizi-Bahktar
authors.forename: J
authors.initials: J
authors.lastname: Mendez-Campos
authors.forename: L
authors.initials: L
authors.lastname: Raju
authors.forename: N A
authors.initials: NA
authors.lastname: Khalique
authors.forename: E
authors.initials: E
authors.lastname: Jubeli
authors.forename: H
authors.initials: H
authors.lastname: Larsen
authors.forename: D
authors.initials: D
authors.lastname: Nicholson
authors.forename: M D
authors.initials: MD
authors.lastname: Pungente
authors.forename: T M
authors.initials: TM
authors.lastname: Fyles
mesh_terms: D000818:Animals; D016466:CHO Cells; D002412:Cations / Q000737:chemistry / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D003412:Cricetulus; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D005696:Galactosidases / Q000235:genetics*; D008055:Lipids / Q000737:chemistry* / Q000633:toxicity; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D011726:Pyridinium Compounds / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection / Q000379:methods
chemical_list: D002412:Cations; D008055:Lipids; D011726:Pyridinium Compounds; D004247:DNA; D005696:Galactosidases