title: Nanosized Fe3O4 an efficient PCR yield enhancer-Comparative study with Au, Ag nanoparticles.
abstract: Nanomaterials-assisted PCR is a promising field of nanobiotechnology that amalgamates nanomaterials into the conventional PCR system to achieve better amplification of desired product. With literature documenting the variable effects of these nanomaterials on the PCR yield and amplification; it was thought worthwhile to compare the PCR enhancing efficiency of three transition metal nanoparticles in form of stable colloidal suspensions at varying concentrations.The nanoparticles(NPs) of silver, gold and magnetite were chemically synthesized by reducing their respective salts and characterized using UV-vis spectroscopy. Their morphology was assessed using nanoparticle tracking system and AFM. The effect of these nanofluids on amplification of 800 bp prokaryotic DNA template with 30% GC content was studied using conventional thermal cycler. The reaction kinetics for all the three nanofluids yielded a Gaussian curve of amplification with varying concentrations. The ammonium salt of oleic acid coated magnetite (Fe3O4) nanoparticles at a concentration of 0.72 × 10(-2)nM and average size of 33 nm demonstrated highest amplification efficiency of 190% as compared to the citrate stabilized AgNP-25 nm (45%) and AuNP-15.19 nm (134%) using a conventional PCR system. The major reasons that allow Fe3O4 NPs outperform the other 2 transition metal NP's seem to be attributed to its heat conduction property as well as effective adsorption of PCR components onto the ammonium salt of oleic acid coated magnetite nanofluids. The data from our study offers valuable information for the application of ferrofluids as economically, efficient and effective alternative for nanomaterial-assisted PCR yield enhancers.
authors.affiliation: Department of Biotechnology, University of Mumbai, Vidyanagari, Kalina, Santacruz (E), Mumbai-98, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Kambli
authors.affiliation: Department of Biotechnology, University of Mumbai, Vidyanagari, Kalina, Santacruz (E), Mumbai-98, India. Electronic address: drvkelkar@mu.ac.in.
authors.forename: Varsha
authors.initials: V
authors.lastname: Kelkar-Mane
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D005290:Ferric Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010316:Particle Size; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012706:Serratia marcescens / Q000235:genetics; D012834:Silver / Q000737:chemistry*; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D004269:DNA, Bacterial; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D012834:Silver; D006046:Gold
keywords: Gold; Magnetite; PCR efficiency; Silver nanoparticles