title: Bioprospecting Chemical Diversity and Bioactivity in a Marine Derived Aspergillus terreus.
abstract: A comparative metabolomic study of a marine derived fungus (Aspergillus terreus) grown under various culture conditions is presented. The fungus was grown in eleven different culture conditions using solid agar, broth cultures, or grain based media (OSMAC). Multivariate analysis of LC/MS data from the organic extracts revealed drastic differences in the metabolic profiles and guided our subsequent isolation efforts. The compound 7-desmethylcitreoviridin was isolated and identified, and is fully described for the first time. In addition, 16 known fungal metabolites were also isolated and identified. All compounds were elucidated by detailed spectroscopic analysis and tested for antibacterial activities against five human pathogens and tested for cytotoxicity. This study demonstrates that LC/MS based multivariate analysis provides a simple yet powerful tool to analyze the metabolome of a single fungal strain grown under various conditions. This approach allows environmentally-induced changes in metabolite expression to be rapidly visualized, and uses these differences to guide the discovery of new bioactive molecules.
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, Oregon 97331, USA, (phone: +01 541-737-1690).
authors.forename: Donovon A
authors.initials: DA
authors.lastname: Adpressa
authors.affiliation: Department of Chemistry, Oregon State University, Corvallis, Oregon 97331, USA, (phone: +01 541-737-1690). sandra.loesgen@oregonstate.edu.
authors.forename: Sandra
authors.initials: S
authors.lastname: Loesgen
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001230:Aspergillus / Q000737:chemistry* / Q000254:growth & development; D001313:Aurovertins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D001688:Biological Products / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D065132:Bioprospecting; D002853:Chromatography, Liquid; D006801:Humans; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001313:Aurovertins; D001688:Biological Products; D009183:Mycotoxins; C014416:citreoviridin
keywords: Antibiotic activity; Aspergillus terreus; Cytotoxic activity; Endophytic fungi; OSMAC; Principal component analysis; Terrein; α-Pyrone mycotoxins