title: Puerarin accelerate scardiac angiogenesis and improves cardiac function of myocardial infarction by upregulating VEGFA, Ang-1 and Ang-2 in rats.
abstract: OBJECTIVE
  The traditional Chinese medicinal puerarin, has long been used to treat cardiovascular diseases, however, the mechanism underlying its effects remain unclear. Here, this study would to investigate the role of puerarin on cardiac angiogenesis and myocardial function induced by myocardial infarction.
  
  
  METHODS
  Puerarin was treated in rats after left anterior descending coronary artery (LAD) ligation and maintained for 4 weeks (diets containing about 50 mg/kg/day or 100 mg/kg/day). After treatment, cardiac function was evaluated by echocardiography and markers of heart failure. Paraffin sections of the heart tissues were used for isolect in GS-IB4 staining. The Mrna and protein expression levels of VEGFA, Ang-1 and Ang-2 were detected by real-time polymerase chain reaction and western blot.
  
  
  RESULTS
  Significantly damaged angiogenesis and slightly increase of VEGFA, Ang-1 and Ang-2 were showed after LAD ligation. Impaired angiogenesis and cardiac function were remarkably improved in puerarin treatment rats with great increase of VEGFA, Ang-1 and Ang-2.
  
  
  CONCLUSIONS
  The above results demonstrated that puerarin could accelerate cardiac angiogenesis and improve cardiac function of myocardial infarction rats by upregulating VEGFA, Ang-1 and Ang-2.
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Ai
authors.affiliation: Department of Cardiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Manhua
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Guizhong
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gui
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Xiangyan
authors.initials: X
authors.lastname: Bai
authors.affiliation: Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
keywords: Puerarin; VEGFA; angiopoietin; cardiac angiogenesis; cardiac function