title: Exopolysaccharides of Lactobacillus reuteri: Their influence on adherence of E. coli to epithelial cells and inflammatory response.
abstract: The aim of the study was to characterize exopolysaccharides (EPS) originated from Lactobacillus reuteri strain DSM 17938 (EPS-DSM17938) and L. reuteri strain L26 Biocenol™ (EPS-L26) and evaluate their influence on adherence of enterotoxigenic Escherichia coli (ETEC) to IPEC-1 cells and proinflammatory gene expression. Both EPS were d-glucan polysaccharides with higher molecular weight (Mw), but differing in spatial conformation and elicited variable cytokine profile. EPS-DSM17938, relatively linear polysaccharide with (1→4) and (1→6) glycosidic linkages, increased IL-1β gene expression (0.1mg/mL; P<0.05), while EPS-L26, more branched polysaccharide with (1→3) and (1→6) glycosidic linkages, exerted slight but statistically significant up-regulation of NF-κB, TNF-α and IL-6 mRNA (P<0.05). The most significant finding is that preincubation of IPEC-1 cells with both EPS followed by ETEC infection inhibit ETEC adhesion on IPEC-1 cells (P<0.01) and ETEC-induced gene expression of proinflammatory cytokine IL-1β and IL-6 (P<0.01).
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: Petra
authors.initials: P
authors.lastname: Kšonžeková
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Bystrický
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: Silvia
authors.initials: S
authors.lastname: Vlčková
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Pätoprstý
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: Lucia
authors.initials: L
authors.lastname: Pulzová
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Mudroňová
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: Terézia
authors.initials: T
authors.lastname: Kubašková
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Csank
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia. Electronic address: ludmila.tkacikova@uvlf.sk.
authors.forename: Ľudmila
authors.initials: Ľ
authors.lastname: Tkáčiková
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D005936:Glucans / Q000032:analysis; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D005936:Glucans; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D011135:Polysaccharides, Bacterial; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha
keywords: Adherence; ETEC; Exopolysaccharides; Immunomodulation; Lactobacillus reuteri