title: Aluminum trichloride inhibits osteoblastic differentiation through inactivation of Wnt/β-catenin signaling pathway in rat osteoblasts.
abstract: Exposure to aluminum (Al) suppresses bone formation. Osteoblastic differentiation plays a key role in the process of bone formation. However, the effect of Al on osteoblastic differentiation is still controversial, and the mechanism remains unclear. To investigate the effect of Al on osteoblastic differentiation and whether Wnt signaling pathway was involved in it, the primary rat osteoblasts were exposed to 1/40 IC50, 1/20 IC50 and 1/10 IC50 of aluminum trichloride (AlCl3) for 24h, respectively. The activity analysis of alkaline phosphate, qRT-PCR analysis of type I collagen, alkaline phosphate, Wnt3a and Dkk-1, Western blot analysis of p-GSK3β, GSK3β and β-catenin protein and Immunofluorescence staining for β-catenin suggested that AlCl3 inhibited osteoblastic differentiation and Wnt/β-catenin pathway. Moreover, we found exogenous Wnt3a application reversed the inhibitory effect of AlCl3 on osteoblastic differentiation, accompanied by activating the Wnt/β-catenin pathway. Taken together, these findings suggest that AlCl3 inhibites osteoblastic differentiation through inactivation of Wnt/β-catenin pathway in osteoblasts.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai Street, Xiangfang District, Harbin 150030, China.
authors.forename: Feibo
authors.initials: F
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, NO. 59 Mucai Street, Xiangfang District, Harbin 150030, China. Electronic address: liyanfei@neau.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Li
mesh_terms: D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002712:Chlorides / Q000633:toxicity*; D024042:Collagen Type I / Q000378:metabolism; D015386:Hazardous Substances / Q000633:toxicity*; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D024042:Collagen Type I; D015386:Hazardous Substances; D000077410:Aluminum Chloride
keywords: Aluminum trichloride; Osteoblastic differentiation; Rat osteoblasts; Wnt/β-catenin pathway