title: Discrepancy in Expression of β-Secretase and Amyloid-β Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia.
abstract: Brain ischemia may be causally related with Alzheimer's disease. Presumably, β-secretase and amyloid-β protein precursor gene expression changes may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both β-secretase and amyloid-β protein precursor genes in the medial temporal lobe cortex with the use of quantitative rtPCR analysis following 10-min global brain ischemia in rats with survival of 2, 7, and 30 days. The greatest significant overexpression of β-secretase gene was noted on the 2nd day, while on days 7-30 the expression of this gene was only modestly downregulated. Amyloid-β protein precursor gene was downregulated on the 2nd day, but on days 7-30 postischemia, there was a significant reverse tendency. Thus, the demonstrated alterations indicate that the considerable changes of expression of β-secretase and amyloid-β protein precursor genes may be connected with a response of neurons in medial temporal lobe cortex to transient global brain ischemia. Finally, the ischemia-induced gene changes may play a key role in a late and slow onset of Alzheimer-type pathology.
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Pluta
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocki
authors.forename: Marzena
authors.initials: M
authors.lastname: Ułamek-Kozioł
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Petniak
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Gil-Kulik
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Januszewski
authors.affiliation: Warsaw Higher Humanistic School, Warsaw, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bogucki
authors.affiliation: Department of Rehabilitation and Orthopaedics, Medical University of Lublin, Lublin, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Jabłoński
authors.affiliation: Department of Clinical Psychology, Medical University of Lublin, Lublin, Poland.
authors.forename: Judyta
authors.initials: J
authors.lastname: Brzozowska
authors.affiliation: Department of Neonate and Infant Pathology, Medical University of Lublin, Lublin, Poland.
authors.forename: Wanda
authors.initials: W
authors.lastname: Furmaga-Jabłońska
authors.affiliation: Department of Pharmaceutical Botany, Medical University of Lublin, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Bogucka-Kocka
authors.affiliation: Department of Pathophysiology, Medical University of Lublin, Lublin, Poland.
authors.forename: Stanisław J
authors.initials: SJ
authors.lastname: Czuczwar
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000235:genetics / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002545:Brain Ischemia / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology*; D005260:Female; D051381:Rats; D017208:Rats, Wistar; D013702:Temporal Lobe / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016564:Amyloid beta-Protein Precursor; D053829:Amyloid Precursor Protein Secretases
keywords: Alzheimer’s disease; amyloid-β protein precursor; brain ischemia; dementia; temporal cortex; β-secretase