title: Neocortical slices from adult chronic epileptic rats exhibit discharges of higher voltages and broader spread.
abstract: Much of the current understanding of epilepsy mechanisms has been built on data recorded with one or a few electrodes from temporal lobe slices of normal young animals stimulated with convulsants. Mechanisms of adult, extratemporal, neocortical chronic epilepsy have not been characterized as much. A more advanced understanding of epilepsy mechanisms can be obtained by recording epileptiform discharges simultaneously from multiple points of an epileptic focus so as to define their sites of initiation and pathways of spreading. Brain slice recordings can characterize epileptic mechanisms in a simpler, more controlled preparation than in vivo. Yet, the intrinsic hyper-excitability of a chronic epileptic focus may not be entirely preserved in slices following the severing of connections in slice preparation. This study utilizes recordings of multiple electrode arrays to characterize which features of epileptic hyper-excitability present in in vivo chronic adult neocortical epileptic foci are preserved in brain slices. After tetanus toxin somatosensory cortex injections, adult rats manifest chronic spontaneous epileptic discharges both in the injection site (primary focus) and in the contralateral side (secondary focus). We prepared neocortical slices from these epileptic animals. When perfused with 4-Aminopyridine in a magnesium free medium, epileptic rat slices exhibit higher voltage discharges and broader spreading than control rat slices. Rates of discharges are similar in slices of epileptic and normal rats, however. Ictal and interictal discharges are distributed over most cortical layers, though with significant differences between primary and secondary foci. A chronic neocortical epileptic focus in slices does not show increased spontaneous pacemakers initiating epileptic discharges but shows discharges with higher voltages and broader spread, consistent with an enhanced synchrony of cellular and synaptic generators over wider surfaces.
authors.affiliation: Department of Neurology, University of Utah, Clinical Neuroscience Center, George E. Wahlen VA Medical Center, Salt Lake City, UT, United States. Electronic address: ruggero.serafini@yahoo.com.
authors.forename: R
authors.initials: R
authors.lastname: Serafini
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, United States.
authors.forename: S
authors.initials: S
authors.lastname: Dettloff
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, United States; Department of Neurology and Rehabilitation, University of Illinois at Chicago, Chicago, IL, United States.
authors.forename: J A
authors.initials: JA
authors.lastname: Loeb
mesh_terms: D015761:4-Aminopyridine; D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D000069280:Electrocorticography; D004827:Epilepsy / Q000503:physiopathology*; D066298:In Vitro Techniques; D008274:Magnesium; D008297:Male; D019579:Neocortex / Q000503:physiopathology*; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000503:physiopathology; D013744:Tetanus Toxin; D046509:Tissue Culture Techniques
chemical_list: D013744:Tetanus Toxin; D015761:4-Aminopyridine; D008274:Magnesium
keywords: brain slices; chronic epilepsy; neocortical epilepsy