title: Effect of magnesium supplementation on blood rheology in NOS inhibition-induced hypertension model.
abstract: This study investigated the effects of magnesium on blood rheological properties and blood pressure in nitric oxide synthase (NOS) inhibition-induced hypertension model. Hypertension was induced by oral administration of the nonselective NOS inhibitor N-nitro-L-arginine methyl ester (L-NAME, 25 mg/kg/day) for 6 weeks and systolic blood pressure was measured by the tail-cuff method. The groups receiving magnesium supplementation were fed with rat chow containing 0.8% magnesium oxide during the experiment. At the end of experiment, blood samples were obtained from abdominal aorta, using ether anesthesia. Plasma and erythrocyte magnesium levels were determined by the atomic absorption spectrometer. RBC deformability and aggregation were determined by rotational ektacytometry. Plasma fibrinogen concentration was evaluated by ELISA. Whole blood and plasma viscosities were determined by viscometer and intracellular free Ca++ level was measured by using spectroflurometric method. Blood pressure was elevated in hypertensive groups and suppressed by magnesium therapy. Plasma viscosity and RBC aggregation were found to be higher in hypertensive rats than control animals and these parameters significantly decreased in magnesium supplemented hypertensive animals. Other measurements were not different between experimental groups. These results confirm that blood pressure, plasma viscosity and RBC aggregation increased in NOS inhibition-induced hypertension model and oral magnesium supplementation improved these parameters.
authors.affiliation: Akdeniz University, Faculty of Medicine, Department of Anaesthesiology and Reanimation, Antalya, Turkey.
authors.forename: Melike
authors.initials: M
authors.lastname: Cengiz
authors.affiliation: Akdeniz University, Faculty of Medicine, Department of Physiology, Antalya, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Ülker
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Physiology, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Üyüklü
authors.affiliation: Akdeniz University, Faculty of Medicine, Department of Biophysics, Antalya, Turkey.
authors.forename: Nazmi
authors.initials: N
authors.lastname: Yaraş
authors.affiliation: Akdeniz University, Faculty of Medicine, Department of Physiology, Antalya, Turkey.
authors.forename: Nur
authors.initials: N
authors.lastname: Özen
authors.affiliation: Akdeniz University, Faculty of Medicine, Department of Medical Biochemistry, Antalya, Turkey.
authors.forename: Mutay
authors.initials: M
authors.lastname: Aslan
authors.affiliation: Istanbul Technical University, Department of Chemistry, Faculty of Sciences and Letters, Istanbul, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Özyurt
authors.affiliation: Akdeniz University, Faculty of Medicine, Department of Physiology, Antalya, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Basralı
mesh_terms: D000818:Animals; D006973:Hypertension / Q000097:blood / Q000139:chemically induced*; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012212:Rheology
chemical_list: D019001:Nitric Oxide Synthase; D008274:Magnesium
keywords: Hypertension; blood rheology; magnesium supplementation