title: Energy biotechnology in the CRISPR-Cas9 era.
abstract: The production of bioenergy from plant biomass previously relied on using microorganisms that rapidly and efficiently convert simple sugars into fuels and chemicals. However, to exploit the far more abundant carbon fixed in plant cell walls, future industrial production hosts will need to be engineered to leverage the most efficient biochemical pathways and most robust traits that can be found in nature. The CRISPR-Cas9 genome editing technology now enables writing the genome at will, which will allow biotechnology to become an 'information science.' This review covers recent advances in using CRISPR-Cas9 to engineer the genomes of a wide variety of organisms that could be use in the industrial production of biofuels and renewable chemicals.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
authors.forename: Raissa
authors.initials: R
authors.lastname: Estrela
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA; Department of Chemistry, University of California, Berkeley 94720, USA; Molecular Biophysics and Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley 94720, USA. Electronic address: jcate@lbl.gov.
authors.forename: Jamie Harrison Doudna
authors.initials: JH
authors.lastname: Cate
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D064130:CRISPR-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D000072669:Gene Editing / Q000379:methods*; D016678:Genome
chemical_list: D056804:Biofuels; D064130:CRISPR-Associated Proteins