title: An integrated microfluidic chip for immunomagnetic detection and isolation of rare prostate cancer cells from blood.
abstract: The quantitative and qualitative analysis of circulating tumor cells (CTCs) has the potential to improve the clinical management of several cancers, including prostate cancer. As such, there is much interest in the isolation of CTCs from the peripheral blood of cancer patients. We report the design, fabrication, and proof-of-principle testing of an integrated permalloy-based microfluidic chip for immunomagnetic isolation of blood-borne prostate cancer cells using an antibody targeting prostate surface membrane antigen (PSMA). The preliminary results using spiked blood samples indicate that the proposed device is consistently capable of isolating prostate cancer cells with high sensitivity (up to 98 %) at clinically relevant low concentrations (down to 20 cells/mL) and an acceptable throughput (100 μL/min).
authors.affiliation: School of Mechatronic Systems Engineering, Simon Fraser University, 250-13450 102nd Avenue, Surrey, BC, V3T 0A3, Canada.|Faculty of Health Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada.
authors.forename: Hadi
authors.initials: H
authors.lastname: Esmaeilsabzali
authors.affiliation: Faculty of Health Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada.
authors.forename: Timothy V
authors.initials: TV
authors.lastname: Beischlag
authors.affiliation: The Vancouver Prostate Centre, Vancouver Coastal Health Research Institute, Jack Bell Research Centre, 2660 Oak Street, Vancouver, BC, V6H 3Z6, Canada.|Department of Urological Sciences, University of British Columbia, Jack Bell Research Centre, 2660 Oak Street, Vancouver, BC, V6H 3Z6, Canada.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Cox
authors.affiliation: Department of Mechanical Engineering, University of Victoria, 3800 Finnerty Rd, Victoria, BC, V8W 3P6, Canada.
authors.forename: Nikolai
authors.initials: N
authors.lastname: Dechev
authors.affiliation: School of Engineering Science, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada.
authors.forename: Ash M
authors.initials: AM
authors.lastname: Parameswaran
authors.affiliation: School of Mechatronic Systems Engineering, Simon Fraser University, 250-13450 102nd Avenue, Surrey, BC, V3T 0A3, Canada. ed_park@sfu.ca.|Faculty of Health Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, V5A 1S6, Canada. ed_park@sfu.ca.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Park
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods; D056656:Lab-On-A-Chip Devices; D008297:Male; D009360:Neoplastic Cells, Circulating; D011471:Prostatic Neoplasms / Q000097:blood*; D051381:Rats
keywords: Circulating tumor cells; Immunomagnetic; Microfluidic; PSMA; Permalloy; Prostate cancer