title: Targeting ofNHERF1through RNA interference inhibits the proliferation and migration of metastatic prostate cancer cells.
abstract: The present study aimed to investigate the effects of Na+/H+ exchanger regulatory factor 1 (NHERF1) gene knockdown, using short-hairpin RNA (shRNA), on the malignant behaviors of prostate cancer cells. A pSuper.puroNHERF1shRNA vector was transfected into PC-3M prostate cancer cells using Lipofectamine 2000. Stable cell lines were obtained andNHERF1knockdown was verified through western blot analysis. MTT assays were then used to measure PC-3M cell proliferation; in addition, cell migration was assessed using a wound healing assay. Flow cytometry was employed in order to determine the effects ofNHERF1knockdown on apoptosis. Expression levels of apoptotic pathway proteins B cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein were then determined by western blot analysis. The results demonstrated that shRNA knockdown ofNHERF1significantly suppressed the proliferation of PC-3M cells by >50%. In addition, knockdown ofNHERF1significantly inhibited the migration of PC-3M cells. PC-3M cells harboringNHERF1shRNA exhibited significantly increased apoptosis, with an ~4-fold increase compared with that of the parental PC-3M cells and cells transfected with an empty vector. Furthermore, the results revealed that knockdown ofNHERF1reduced the protein expression of Bcl-2, although the expression of Bax was unaltered. In conclusion,NHERF1knockdown using shRNA inhibited the proliferation and migration of PC-3M cells and promoted apoptosis, highlighting the role ofNHERF1in prostate cancer progression.
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing 100081, P.R. China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, P.R. China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of Molecular Biology, Institute of Medical Biology, Peking Union Medical College and Chinese Academy of Medical Science, Kunming, Yunnan 650000, P.R. China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, P.R. China.
authors.forename: Nenan
authors.initials: N
authors.lastname: Lyu
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, P.R. China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Gao
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, P.R. China.
authors.forename: Deguan
authors.initials: D
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Junfang
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy of Medical Science, Tianjin 300192, P.R. China.
authors.forename: Naling
authors.initials: N
authors.lastname: Song
keywords: Na+/H+ exchanger regulatory factor 1; apoptosis; prostate cancer