title: Enhancement in xylose utilization using Kluyveromyces marxianus NIRE-K1 through evolutionary adaptation approach.
abstract: The evolutionary adaptation was carried out on the thermotolerant yeast Kluyveromyces marxianus NIRE-K1 at 45 °C up to 60 batches to enhance its xylose utilization capability. The adapted strain showed higher specific growth rate and 3-fold xylose uptake rate and short lag phase as compared to the native strain. During aerobic growth adapted yeast showed 2.81-fold higher xylose utilization than that of native. In anaerobic batch fermentation, adapted yeast utilized about 91% of xylose in 72 h and produced 2.88 and 18.75 g l⁻¹ of ethanol and xylitol, respectively, which were 5.11 and 5.71-fold higher than that of native. Ethanol yield, xylitol yield and specific sugar consumption rate obtained by the adapted cells were found to be 1.57, 1.65 and 4.84-fold higher than that of native yeast, respectively. Aforesaid results suggested that the evolutionary adaptation will be a very effective strategy in the near future for economic lignocellulosic ethanol production.
authors.affiliation: Biochemical Conversion Division, Sardar Swaran Singh National Institute of Bio-Energy, Kapurthala, India.|I.K Gujral Punjab Technical University, Kapurthala, India.
authors.forename: Nilesh Kumar
authors.initials: NK
authors.lastname: Sharma
authors.affiliation: Biochemical Conversion Division, Sardar Swaran Singh National Institute of Bio-Energy, Kapurthala, India.
authors.forename: Shuvashish
authors.initials: S
authors.lastname: Behera
authors.affiliation: Biochemical Conversion Division, Sardar Swaran Singh National Institute of Bio-Energy, Kapurthala, India.|I.K Gujral Punjab Technical University, Kapurthala, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Arora
authors.affiliation: Biochemical Conversion Division, Sardar Swaran Singh National Institute of Bio-Energy, Kapurthala, India. sachin.biotech@gmail.com.
authors.forename: Sachin
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D005075:Biological Evolution; D005285:Fermentation; D007700:Kinetics; D007716:Kluyveromyces / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D014994:Xylose
keywords: Ethanol; Evolutionary adaptation; Kluyveromyces marxianus; Lignocelluloses; Xylitol; Xylose