title: Recognition and detection of 8-oxo-rG in RNA using the DNA/OMeRNA chimera probes containing fluorescent adenosine-diazaphenoxazine analog.
abstract: Recent studies indicate that oxidative damage to RNA results in dysfunction of translation and eventual pathogenesis. A representative oxidized base in RNA is 8-hydroxyguanosine (8-oxo-rG), however, unlike its DNA counterpart (8-oxo-dG), its role in pathogenesis has not attracted much attention until recently. The 2'-deoxyadenosine derivative with a diazaphenoxazine skeleton at the 6-amino group (Adap) was shown to be selective for 8-oxo-dG in DNA. In this study, the 2'-O-methoxy derivative of Adap (2'-OMeAdap) was designed as a selective molecule for 8-oxo-rG in RNA. 8-Oxo-rG in the homopurine RNA was selectively recognized by the ODN probe incorporating Adap. In contrast, although it was not possible by the Adap-containing ODN prove due to the instability of the corresponding duplex, 8-oxo-rG in homopyrimidine RNA was selectively detected by the 2'-OMeRNA probe incorporating 2'-OMeAdap.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Koga
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: taniguch@phar.kyushu-u.ac.jp.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Taniguchi
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Kikukawa
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: sasaki@phar.kyushu-u.ac.jp.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D000241:Adenosine / Q000032:analysis / Q000737:chemistry*; D001372:Aza Compounds / Q000032:analysis / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry*; D010078:Oxazines / Q000032:analysis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
chemical_list: C000601117:1,3-diazaphenoxazine; D001372:Aza Compounds; D005456:Fluorescent Dyes; D015335:Molecular Probes; D010078:Oxazines; D006151:Guanosine; C046215:8-hydroxyguanosine; D012313:RNA; D004247:DNA; D000241:Adenosine
keywords: 8-Oxo-guanosine; Chimera probe; Fluorescence quenching; Fluorescent nucleoside analog; Oxidative damage