title: Effect of oxygen pressure during incubation with a (10)B-carrier on (10)B uptake capacity of cultured p53 wild-type and mutated tumor cells: dependency on p53 status of tumor cells and types of (10)B-carriers.
abstract: Purpose To evaluate the effect of oxygen pressure during incubation with a (10)B-carrier on (10)B uptake capacity of cultured p53 wild-type and mutated tumor cells. Materials and methods Cultured human head and neck squamous cell carcinoma cell line transfected with mutant TP53 (SAS/mp53), or with a neo vector as a control (SAS/neo) was incubated with L-para-boronophenylalanine-(10)B (BPA) or sodium mercaptoundecahydrododecaborate-(10)B (BSH) as a (10)B-carrier at the (10)B concentration of 60 ppm for 24 h under aerobic (20.7% of oxygen) or hypoxic (0.28% of oxygen) conditions. Immediately after incubation, cultured tumor cells received reactor thermal neutron beams, and a cell survival assay was performed. (10)B concentration of cultured SAS/neo or SAS/mp53 cells incubated under aerobic or hypoxic conditions was determined with a thermal neutron guide tube. Results Hypoxic incubation significantly decreased (10)B concentration of cultured cells with a clearer tendency observed following BPA than BSH treatment in both SAS/neo and SAS/mp53 cells. Following neutron beam irradiation, SAS/mp53 cells showed significantly higher relative biological effectiveness values than SAS/neo cells because of the significantly lower radiosensitivity of SAS/mp53 to γ-rays than SAS/neo cells. Conclusion Oxygen pressure during incubation with a (10)B-carrier had a critical impact on (10)B uptake of cultured tumor cells.
authors.affiliation: a Particle Radiation Biology, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan ;
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Masunaga
authors.affiliation: b Department of Radiation Oncology , Kinki University School of Medicine , Ohno-Higashi, Osaka-Sayama , Osaka , Japan ;
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tatebe
authors.affiliation: b Department of Radiation Oncology , Kinki University School of Medicine , Ohno-Higashi, Osaka-Sayama , Osaka , Japan ;
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Nishimura
authors.affiliation: a Particle Radiation Biology, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan ;
authors.forename: Keizo
authors.initials: K
authors.lastname: Tano
authors.affiliation: a Particle Radiation Biology, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan ;
authors.forename: Yu
authors.initials: Y
authors.lastname: Sanada
authors.affiliation: a Particle Radiation Biology, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan ;
authors.forename: Takahiro
authors.initials: T
authors.lastname: Moriwaki
authors.affiliation: c Radiation Medical Physics, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan ;
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sakurai
authors.affiliation: c Radiation Medical Physics, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan ;
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: d Particle Radiation Oncology, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: d Particle Radiation Oncology, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kondo
authors.affiliation: c Radiation Medical Physics, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan ;
authors.forename: Akira
authors.initials: A
authors.lastname: Maruhashi
authors.affiliation: d Particle Radiation Oncology, Research Reactor Institute , Kyoto University , Kumatori, Osaka , Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ono
mesh_terms: D001895:Boron / Q000493:pharmacokinetics* / Q000627:therapeutic use*; D016754:Boron Neutron Capture Therapy / Q000379:methods; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000532:radiotherapy*; D002470:Cell Survival / Q000528:radiation effects; D004337:Drug Carriers / Q000737:chemistry; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000532:radiotherapy*; D006801:Humans; D007554:Isotopes / Q000493:pharmacokinetics / Q000627:therapeutic use; D009154:Mutation; D010100:Oxygen / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics / Q000627:therapeutic use; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D007554:Isotopes; D019275:Radiopharmaceuticals; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D001895:Boron; D010100:Oxygen
keywords: 10B-carrier; Oxygen pressure; boron neutron capture reaction; p53 status