title: Chemical composition, pretreatments and saccharification of Senna siamea (Lam.) H.S. Irwin & Barneby: An efficient biomass producing tree legume.
abstract: Protocols were developed for efficient release of glucose from the biomass of Senna siamea, one of the highly efficient biomass producing tree legumes. Composition of mature, 1year and 2years coppice biomass were analysed. For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45min incubation at 50°C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β-glucosidase per gram of glucan. Of the biomass of different particle sizes (40-200mesh) used for saccharification, 40-60mesh shown the maximum glucose release. COSLIF pretreated mature, 1year and 2years coppice biomass showed equivalent glucose release profiles.
authors.affiliation: Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751015, Odisha, India.
authors.forename: Nitesh K
authors.initials: NK
authors.lastname: Mund
authors.affiliation: Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751015, Odisha, India.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Dash
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology, Guwahati 781039, Assam, India.
authors.forename: Chitta R
authors.initials: CR
authors.lastname: Barik
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology, Guwahati 781039, Assam, India.
authors.forename: Vaibhav V
authors.initials: VV
authors.lastname: Goud
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati 781039, Assam, India; Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati 781039, Assam, India.
authors.forename: Lingaraj
authors.initials: L
authors.lastname: Sahoo
authors.affiliation: AICRP on Agroforestry, College of Forestry, Orissa University of Agriculture and Technology, Bhubaneswar 751016, Odisha, India.
authors.forename: Prasannajit
authors.initials: P
authors.lastname: Mishra
authors.affiliation: Regional Plant Resource Centre, Nayapalli, Bhubaneswar 751015, Odisha, India. Electronic address: niharnayak@yahoo.com.
authors.forename: Nihar R
authors.initials: NR
authors.lastname: Nayak
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods; D007887:Fabaceae / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D010316:Particle Size; D010756:Phosphoric Acids / Q000494:pharmacology; D012997:Solvents; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005936:Glucans; D010756:Phosphoric Acids; D012997:Solvents; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C030242:phosphoric acid; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose
keywords: Bioethanol; Biomass composition; Pretreatments; Saccharification; Senna siamea