title: Experimental Colitis Is Attenuated by Cardioprotective Diet Supplementation That Reduces Oxidative Stress, Inflammation, and Mucosal Damage.
abstract: Inflammatory bowel diseases (IBD) such as ulcerative colitis (UC) and Crohn's disease (CD) are multifactorial, relapsing disorders of the gastrointestinal tract. However, the etiology is still poorly understood but involves altered immune responses, epithelial dysfunction, environmental factors, and nutrition. Recently, we have shown that the diet supplement corabion has cardioprotective effects due to reduction of oxidative stress and inflammation. Since oxidative stress and inflammation are also prominent risk factors in IBD, we speculated that corabion also has beneficial effects on experimental colitis. Colitis was induced in male mice by administration of 3.5% (w/v) dextran sulfate sodium (DSS) in drinking water for a period of 3 or 7 days with or without daily gavage feeding of corabion consisting of vitamin C, vitamin E, L-arginine, and eicosapentaenoic and docosahexaenoic acid. We found that corabion administration attenuated DSS-induced colon shortening, tissue damage, and disease activity index during the onset of colitis. Mechanistically, these effects could be explained by reduced neutrophil recruitment, oxidative stress, production of proinflammatory cytokines, and internalization of the junctional proteins ZO-1 and E-cadherin leading to less edema formation. Thus, corabion may be a useful diet supplement for the management of chronic inflammatory intestinal disorders such as IBD.
authors.affiliation: Department of Molecular Biomedicine, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Hilda
authors.initials: H
authors.lastname: Vargas Robles
authors.affiliation: Department of Molecular Biomedicine, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Alí Francisco
authors.initials: AF
authors.lastname: Citalán Madrid
authors.affiliation: Department of Molecular Biomedicine, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Alexander
authors.initials: A
authors.lastname: García Ponce
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Angelica
authors.initials: A
authors.lastname: Silva Olivares
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Mineko
authors.initials: M
authors.lastname: Shibayama
authors.affiliation: Department of Infectomics and Molecular Pathogenesis, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Abigail
authors.initials: A
authors.lastname: Betanzos
authors.affiliation: Department of Pharmacology, National Cardiology Institute "Ignacio Chávez", 14080 Mexico City, DF, Mexico.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Del Valle Mondragón
authors.affiliation: Department of Physiology, Biophysics and Neurosciences, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Porfirio
authors.initials: P
authors.lastname: Nava
authors.affiliation: Department of Molecular Biomedicine, CINVESTAV, Avenida IPN 2508, San Pedro Zacatenco, 07360 Mexico City, DF, Mexico.
authors.forename: Michael
authors.initials: M
authors.lastname: Schnoor
mesh_terms: D022005:Adherens Junctions / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D019587:Dietary Supplements; D007249:Inflammation / Q000473:pathology*; D018836:Inflammation Mediators / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism
chemical_list: D002316:Cardiotonic Agents; D016207:Cytokines; D018836:Inflammation Mediators; D016264:Dextran Sulfate