title: Composition and anti-inflammatory effect of polysaccharides from Sargassum horneri in RAW264.7 macrophages.
abstract: Sulfated polysaccharides extracted from brown marine algae have been shown to possess a variety of biological activities. We assessed the potential activity of the sulfated polysaccharide from Sargassum horneri (SP) and its isolated two major components (fraction-1 (F1) and fraction-2 (F2)), on anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. In the present study, analysis of polysaccharide chemical composition found that the constituent ratios of sulfate ester and fucose in SP and F1 were 4.95% vs 7.6%, and 4.48% vs 55.9%, respectively, suggesting that F1 may be a major sulfated polysaccharide containing fucose. Meanwhile, our findings demonstrated that TNF-α secretion levels were significantly (P<0.05) decreased by SP and F1 treatments in LPS-stimulated RAW264.7 cells in a dose-dependent manner under the preventive and repair experimental models. Pro-/anti-inflammatory (TNF-α/IL-10) cytokines secretion ratios by LPS-stimulated RAW264.7 macrophages were significantly (P<0.05) inhibited by SP and F1 treatments, particularly by F1 (at high dose, 200μg/ml). Moreover, NO release and iNOS activity were significantly (P<0.05) inhibited by F1. Collectively, the present study suggested that purified component, F1 from SP, had strong anti-inflammatory effects on LPS-stimulated RAW264.7 macrophages in the preventive and repair manner through inhibiting TNF-α secretion levels and NO release.
authors.affiliation: Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, China. Electronic address: zswenmr@163.com.
authors.forename: Zheng-Shun
authors.initials: ZS
authors.lastname: Wen
authors.affiliation: Zhejiang Marine Development Research Institute, Zhoushan, Zhejiang 316000, China.
authors.forename: Xing-Wei
authors.initials: XW
authors.lastname: Xiang
authors.affiliation: Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, China.
authors.forename: Huo-Xi
authors.initials: HX
authors.lastname: Jin
authors.affiliation: Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Guo
authors.affiliation: Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, China.
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
authors.forename: Yan-Na
authors.initials: YN
authors.lastname: Huang
authors.affiliation: Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, China.
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: OuYang
authors.affiliation: Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products, Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, Zhejiang 316000, China. Electronic address: youle1960@163.com.
authors.forename: You-Le
authors.initials: YL
authors.lastname: Qu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D016753:Interleukin-10 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells / Q000187:drug effects; D044647:Sargassum / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide
keywords: Anti-inflammatory; RAW264.7 macrophages; Sargassum horneri polysaccharides