title: Electrochemical Visualization of Intracellular Hydrogen Peroxide at Single Cells.
abstract: In this Letter, the electrochemical visualization of hydrogen peroxide inside one cell was achieved first using a comprehensive Au-luminol-microelectrode and electrochemiluminescence. The capillary with a tip opening of 1-2 μm was filled with the mixture of chitosan and luminol, which was coated with the thin layers of polyvinyl chloride/nitrophenyloctyl ether (PVC/NPOE) and gold as the microelectrode. Upon contact with the aqueous hydrogen peroxide, hydrogen peroxide and luminol in contact with the gold layer were oxidized under the positive potential resulting in luminescence for the imaging. Due to the small diameter of the electrode, the microelectrode tip was inserted into one cell and the bright luminescence observed at the tip confirmed the visualization of intracellular hydrogen peroxide. The further coupling of oxidase on the electrode surface could open the field in the electrochemical imaging of intracellular biomolecules at single cells, which benefited the single cell electrochemical detection.
authors.affiliation: The State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210093, China.
authors.forename: Ruiqin
authors.initials: R
authors.lastname: He
authors.affiliation: The State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210093, China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Tang
authors.affiliation: The State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210093, China.
authors.forename: Dechen
authors.initials: D
authors.lastname: Jiang
authors.affiliation: The State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing, Jiangsu 210093, China.
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D055664:Electrochemical Techniques; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D011143:Polyvinyl Chloride / Q000737:chemistry; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D008165:Luminol; D006046:Gold; D011143:Polyvinyl Chloride; D006861:Hydrogen Peroxide