title: Genome wide transcriptome profiling reveals differential gene expression in secondary metabolite pathway of Cymbopogon winterianus.
abstract: Advances in transcriptome sequencing provide fast, cost-effective and reliable approach to generate large expression datasets especially suitable for non-model species to identify putative genes, key pathway and regulatory mechanism. Citronella (Cymbopogon winterianus) is an aromatic medicinal grass used for anti-tumoral, antibacterial, anti-fungal, antiviral, detoxifying and natural insect repellent properties. Despite of having number of utilities, the genes involved in terpenes biosynthetic pathway is not yet clearly elucidated. The present study is a pioneering attempt to generate an exhaustive molecular information of secondary metabolite pathway and to increase genomic resources in Citronella. Using high-throughput RNA-Seq technology, root and leaf transcriptome was analysed at an unprecedented depth (11.7 Gb). Targeted searches identified majority of the genes associated with metabolic pathway and other natural product pathway viz. antibiotics synthesis along with many novel genes. Terpenoid biosynthesis genes comparative expression results were validated for 15 unigenes by RT-PCR and qRT-PCR. Thus the coverage of these transcriptome is comprehensive enough to discover all known genes of major metabolic pathways. This transcriptome dataset can serve as important public information for gene expression, genomics and function genomics studies in Citronella and shall act as a benchmark for future improvement of the crop.
authors.affiliation: Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
authors.forename: Kamalakshi
authors.initials: K
authors.lastname: Devi
authors.affiliation: DBT-AAU Centre, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
authors.forename: Surajit K
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Distributed Information Centre, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
authors.forename: Jagajjit
authors.initials: J
authors.lastname: Sahu
authors.affiliation: Distributed Information Centre, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
authors.forename: Debashis
authors.initials: D
authors.lastname: Panda
authors.affiliation: Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.|Distributed Information Centre, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
authors.forename: Mahendra K
authors.initials: MK
authors.lastname: Modi
authors.affiliation: Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat 785013, Assam, India.
authors.forename: Priyabrata
authors.initials: P
authors.lastname: Sen
mesh_terms: D031743:Cymbopogon / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018745:Genome, Plant; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D013729:Terpenes / Q000378:metabolism
chemical_list: D013729:Terpenes