title: Mammalian interspecies substitution of immune modulatory alleles by genome editing.
abstract: We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele introgression in one generation opens unprecedented opportunities for agriculture and basic research.
authors.affiliation: The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: Simon G
authors.initials: SG
authors.lastname: Lillico
authors.affiliation: The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: Chris
authors.initials: C
authors.lastname: Proudfoot
authors.affiliation: The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: Tim J
authors.initials: TJ
authors.lastname: King
authors.affiliation: The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Tan
authors.affiliation: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA.
authors.forename: Rachel
authors.initials: R
authors.lastname: Mardjuki
authors.affiliation: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA.
authors.forename: David E
authors.initials: DE
authors.lastname: Paschon
authors.affiliation: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Rebar
authors.affiliation: Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA.
authors.forename: Fyodor D
authors.initials: FD
authors.lastname: Urnov
authors.affiliation: Genus plc, 1525 River Rd., DeForest, WI 53532, USA.
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Mileham
authors.affiliation: Genus plc, 1525 River Rd., DeForest, WI 53532, USA.
authors.forename: David G
authors.initials: DG
authors.lastname: McLaren
authors.affiliation: The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.
authors.forename: C Bruce A
authors.initials: CB
authors.lastname: Whitelaw
mesh_terms: D000357:African Swine Fever / Q000235:genetics / Q000821:virology; D000358:African Swine Fever Virus / Q000472:pathogenicity; D000483:Alleles; D000818:Animals; D060467:Disease Resistance / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D005818:Genetic Engineering; D016678:Genome; D006239:Haplotypes; D008025:Ligases / Q000235:genetics*; D013552:Swine
chemical_list: D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases