title: Anti-DNase I antibodies in systemic lupus erythematosus: diagnostic value and share in the enzyme inhibition.
abstract: Diagnostic accuracy of anti-DNase I antibodies measurement in a differentiation between SLE and other autoimmune rheumatic diseases was evaluated. The share of anti-DNase I and actin in the DNase I activity decrease in SLE was established. Serum samples were obtained from 54 patients with verified SLE, 52 control patients with other autoimmune rheumatic diseases, and 44 healthy persons. Anti-DNase I concentrations were measured by ELISA. Free and actin inhibited DNase I activities were evaluated in the fresh serum samples. The appraisal of antibodies and actin effects on DNase I activity was made using multiple regression. Anti-DNase I antibodies were positive in 35 SLE and 8 control patients, without significant difference between the mean antibody concentrations. Sensitivity of this test was 64.81 %, and specificity-84.62 %. Mean free DNase I activity in SLE was somewhat lower than in the control group as a result of augmented frequency of extremely low enzyme activities. On the contrary, after the exclusion of the latter cases we have revealed elevated mean free DNase I activity in the other SLE patients comparing to the similar control subgroup. Unlike the controls, low serum DNase I activity in SLE arose not only from actin and antibody action, but also, in half of the cases, from unidentified factor, related to active SLE. The accuracy of the anti-DNase I antibodies measurement is approximate to the present reference standard of SLE diagnostics. We first demonstrated that neither antibodies nor actin caused DNase I activity decrease in SLE.
authors.affiliation: FSBSI Research Institute of Clinical and Experimental Rheumatology, 76, Zemlyachki Str., Volgograd, Russia, 400138. a.s.trofimenko@mail.ru.|Hospital Therapy Department, Volgograd State Medical University, 1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131. a.s.trofimenko@mail.ru.
authors.forename: A S
authors.initials: AS
authors.lastname: Trofimenko
authors.affiliation: FSBSI Research Institute of Clinical and Experimental Rheumatology, 76, Zemlyachki Str., Volgograd, Russia, 400138.|Hospital Therapy Department, Volgograd State Medical University, 1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131.
authors.forename: I P
authors.initials: IP
authors.lastname: Gontar
authors.affiliation: FSBSI Research Institute of Clinical and Experimental Rheumatology, 76, Zemlyachki Str., Volgograd, Russia, 400138.|Hospital Therapy Department, Volgograd State Medical University, 1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131.
authors.forename: A B
authors.initials: AB
authors.lastname: Zborovsky
authors.affiliation: Hospital Therapy Department, Volgograd State Medical University, 1, Pavshikh Bortsov Sq., Volgograd, Russia, 400131.
authors.forename: O V
authors.initials: OV
authors.lastname: Paramonova
mesh_terms: D000199:Actins / Q000097:blood; D019540:Area Under Curve; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000201:enzymology / Q000276:immunology*; D011237:Predictive Value of Tests; D012372:ROC Curve; D012044:Regression Analysis; D015203:Reproducibility of Results
chemical_list: D000199:Actins; D001323:Autoantibodies; D015415:Biomarkers; D003850:Deoxyribonuclease I
keywords: Antibodies; Deoxyribonuclease I; Sensitivity and specificity; Systemic lupus erythematosus