title: Analysis of bacterial community profiles of endodontically infected primary teeth using pyrosequencing.
abstract: OBJECTIVE
  The purpose of this study was to identify the endodontic microbiome in primary teeth with dental caries using high-throughput pyrosequencing and to establish data on the oral microbiome of primary teeth with endodontic infection.
  
  
  METHODS
  Ten primary teeth with dental caries and endodontic infection were included. Samples were collected from root canals using sterilized paper points and analyzed by pyrosequencing, based on the V1-V3 hypervariable region of the 16S rRNA gene. The data were analyzed using the CLcommunity software.
  
  
  RESULTS
  Analysis of the 10 samples yielded a total of 64,291 16S rRNA gene sequences. In total, 1586 OTUs (range, 91-235), six bacterial phyla, including unclassified, and 187 genera were assigned. At the genus level, Neisseria (group A), Acinetobacter (group B), and Fusobacterium (group C) were prominent. These predominant microorganisms were associated with the clinical condition and reflected the progression of endodontic infection in primary teeth.
  
  
  CONCLUSIONS
  This study revealed a relationship between the oral microbiome and endodontic infection in primary teeth. Additionally, anaerobic bacteria such as Fusobacterium species were dominant in the teeth with apical abscesses.
authors.affiliation: Graduate School, Kyung Hee University, Seoul, Republic of Korea.|Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Kwan Hee
authors.initials: KH
authors.lastname: Yun
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hyo-Seol
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Graduate School, Kyung Hee University, Seoul, Republic of Korea.|Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Ok Hyung
authors.identifier: http://orcid.org/0000-0002-6386-803X
authors.initials: OH
authors.lastname: Nam
authors.affiliation: Graduate School, Kyung Hee University, Seoul, Republic of Korea.|Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Chan Yang
authors.initials: CY
authors.lastname: Moon
authors.affiliation: Department of Life and Nanopharmaceutical Sciences and Department of Maxillofacial Biomedical Engineering, school of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Choi
mesh_terms: D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000382:microbiology* / Q000628:therapy; D003786:Dental Pulp Cavity / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D064307:Microbiota; D008963:Molar; D010485:Periapical Periodontitis / Q000382:microbiology* / Q000628:therapy; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D014094:Tooth, Deciduous
chemical_list: D004269:DNA, Bacterial