title: Bacterial treatment of alkaline cement kiln dust using Bacillus halodurans strain KG1.
abstract: This study was conducted to isolate an acid-producing, alkaliphilic bacterium to reduce the alkalinity of cement industry waste (cement kiln dust). Gram-positive isolate KG1 grew well at pH values of 6-12, temperatures of 28-50°C, and NaCl concentrations of 0-16% and thus was further screened for its potential to reduce the pH of an alkaline medium. Phenotypic characteristics of the KG1 isolate were consistent with those of the genus Bacillus, and the highest level of 16S rRNA gene sequence similarity was found with Bacillus halodurans strain DSM 497 (94.7%). On the basis of its phenotypic characteristics and genotypic distinctiveness from other phylogenetic neighbors belonging to alkaliphilic Bacillus species, the isolated strain was designated B. halodurans strain KG1, with GenBank accession number JQ307184 (= NCIM 5439). Isolate KG1 reduced the alkalinity (by 83.64%) and the chloride content (by 86.96%) of cement kiln dust and showed a potential to be used in the cement industry for a variety of applications.
authors.affiliation: Department of Biotechnology, Thapar University, Patiala, Punjab 147004, India.
authors.lastname: Kunal
authors.affiliation: School of Energy and Environment, Thapar University, Patiala, Punjab 147004, India. Electronic address: anitarajor@yahoo.com.
authors.forename: Anita
authors.initials: A
authors.lastname: Rajor
authors.affiliation: Department of Civil Engineering, Thapar University, Patiala, Punjab 147004, India.
authors.forename: Rafat
authors.initials: R
authors.lastname: Siddique
mesh_terms: D001407:Bacillus / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001709:Biotechnology / Q000379:methods*; D016000:Cluster Analysis; D003252:Construction Materials; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D018505:Waste Management / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: Alkalinity reduction; Alkaliphiles; Bacillus sp.; Cement kiln dust