title: The immune adjuvant response of polysaccharides from Atractylodis macrocephalae Koidz in chickens vaccinated against Newcastle disease (ND).
abstract: Build on our previous research, polysaccharides from the rhizome of Atractylodis macrocephalae Koidz (RAMPS), RAMPStp and RAMPS60c were prepared and the structural characterization and immune response of ND vaccine in chicken were investigated. Immune organ index, Lymphocyte proliferation, antibody titers, cell cycle distribution, and percentages of CD4(+) and CD8(+) cells were determined. GPC analysis showed that the Mn of RAMPS with two peaks were 1.29×10(5) and 1.74×10(3), respectively. GC-MS analysis revealed that RAMPS was composed of glucose, mannose, arabinose, galactose, xylose, d-Ribose and rhamnose, with mass percentages of 66.39%, 21.24%, 5.64%, 2.65%, 2.30%, 1.15% and 0.64%, respectively. NMR spectroscopic analysis demonstrated that a preliminary structure of RAMPS was proposed as 1,3-linked β-d-Galp and 1,6-linked β-d-Galpresidues. In vivo test showed that RAMPStp and RAMPS60c could promote peripheral lymphocytes proliferation and entering into S and G2/M phases, enhance serum HI antibody titer and effectively improve the percentages of CD4(+) and CD8(+) T cells in chickens vaccinated with ND vaccine at most time points. The actions of RAMPStp and RAMPS60c were stronger than that of Lev, and RAMPStp presented the best efficacy. These results indicated that RAMPStp and RAMPS60c characterize of the immune-enhancing activity and RAMPStp possessed the strongest activity. It would be anticipated as a component of new-type immunopotentiator.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Sun
authors.affiliation: Zhengzhou Ninth People's Hospital, Zhengzhou 450053, PR China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Central Hospital of Tai'an City, Tai'an 271018, Shandong, PR China.
authors.forename: Guangju
authors.initials: G
authors.lastname: Meng
authors.affiliation: Central Hospital of Tai'an City, Tai'an 271018, Shandong, PR China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Qi
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Fan
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Yuge
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China. Electronic address: liujz@sdau.edu.cn.
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D049109:Cell Proliferation; D002645:Chickens; D009005:Monosaccharides / Q000032:analysis; D009522:Newcastle disease virus / Q000276:immunology*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D009005:Monosaccharides; D011134:Polysaccharides; D014765:Viral Vaccines
keywords: Atractylodis macrocephalae Koidz; Characterization; Immue response; Immunopotentiator; Polysaccharides