title: Protective effects of adipose-derived stem cells secretome on human dermal fibroblasts from ageing damages.
abstract: BACKGROUND
  Combined effects of intrinsic and extrinsic ageing factors on skin tissue and the therapies have been rarely studied before. ADSCs have gained popularity in anti-ageing field, which may provide promising methods to fight against skin ageing.
  
  
  OBJECTIVE
  To find out the fate of HDFs exposed to intrinsic or extrinsic ageing factors or both of them and further examine the impacts of ADSC-CM on the damaged HDFs.
  
  
  METHODS
  We irradiated HDFs with UVB at different senescent levels, and then treated them with ADSC-CM. After 48 h, we detected cellular proliferative activity, morphology, SA-β-Gal expression, apoptosis, mRNA expression of collagen I, collagen III and elastin.
  
  
  RESULTS
  Intrinsic ageing factors inhibited cellular proliferation, increased senescent ratio and reduced mRNA expression of collagen I, collagen III and elastin, so did UVB, except for its induction of elastin mRNA expression. Furthermore, ADSC-CM treatment can slightly or significantly improve cellular proliferative activity and restore functions both in irradiated and non-irradiated HDFs. Besides, ADSC-CM treatment decreased cellular apoptosis and senescence induced by UVB but had no obvious effect on cellular senescence induced by intrinsic ageing factors. The results were similar in three generations of HDFs, yet in different degrees.
  
  
  CONCLUSIONS
  The results suggest that ADSCs secretome protect HDFs from ageing damages but with some limitations.
authors.affiliation: Department of Plastic Surgery, The First Hospital of China Medical University Shenyang, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Plastic Surgery, The First Hospital of China Medical University Shenyang, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Hand and Foot Microsurgery, Second Hospital of Dalian Medical University Dalian, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Plastic Surgery, The First Hospital of China Medical University Shenyang, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Xv
authors.affiliation: Department of Plastic Surgery, The First Hospital of China Medical University Shenyang, China.
authors.forename: Shuangyi
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000367:Age Factors; D017209:Apoptosis; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000528:radiation effects; D018920:Coculture Techniques; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism; D004549:Elastin / Q000235:genetics / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D005786:Gene Expression Regulation; D006801:Humans; D019899:Paracrine Communication; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D015595:Skin Aging / Q000473:pathology* / Q000528:radiation effects; D013234:Stem Cells / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000166:cytology*; D013997:Time Factors; D014466:Ultraviolet Rays; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D012333:RNA, Messenger; D004549:Elastin
keywords: Adipose-derived stem cells; anti-ageing; human dermal fibroblasts; secretory factors