title: Sphingobacterium griseoflavum sp. nov., isolated from the insect Teleogryllus occipitalis living in deserted cropland.
abstract: A Gram-stain-negative, aerobic, rod-shaped, non-motile bacterial strain, designated SCU-B140T, was isolated from the insect Teleogryllus occipitalis. Phylogenetic analysis on the basis of 16S rRNA gene sequence showed that strain SCU-B140T belonged to the genus Sphingobacterium. Sphingobacterium bambusae KCTC 22814T (97.87 %) was identified as the most closely related phylogenetic neighbour of strain SCU-B140T. The novel strain was able to grow at salt concentrations of 0-4 % (w/v), at temperatures of 10-40 °C, and at a pH of 6.0-9.0. The major cellular fatty acids were iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), C16 : 0, C16 : 0 3-OH, C18 : 0 and C14 : 0. The major polar lipids consisted of phosphatidylethanolamine, three unknown aminophospholipids, an unknown glycolipid and three unknown polar lipids. MK-7 was the major isoprenoid quinone. The DNA G+C content was 41.2 mol%. The DNA-DNA relatedness value between SCU-B140T and S. bambusae KCTC 22814T was found to be 30.15 %. According to these results, strain SCU-B140T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium griseoflavum sp. nov. is proposed. The type strain is SCU-B140T ( = KCTC 42158T = CGMCC 1.12966T).
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Long
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.|Department of Pharmaceutical and Biological Engineering, College of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu University, No. 168 Zhonghe Rd, Longtan Urban Industry Concentrate Zone, 2nd Section, East 3rd Rd, Chengdu, Sichuan 610052, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Key laboratory of Leather Chemistry and engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D018556:Crops, Agricultural; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006135:Gryllidae / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7