title: Whole genome sequence of Klebsiella pneumoniae U25, a hypermucoviscous, multidrug resistant, biofilm producing isolate from India.
abstract: Klebsiella pneumoniae U25 is a multidrug resistant strain isolated from a tertiary care hospital in Chennai, India. Here, we report the complete annotated genome sequence of strain U25 obtained using PacBio RSII. This is the first report of the whole genome of K. pneumoniaespecies from Chennai. It consists of a single circular chromosome of size 5,491,870-bp and two plasmids of size 211,813 and 172,619-bp. The genes associated with multidrug resistance were identified. The chromosome of U25 was found to have eight antibiotic resistant genes [blaOXA-1,blaSHV-28, aac(6')1b-cr,catB3, oqxAB, dfrA1]. The plasmid pMGRU25-001 was found to have only one resistant gene (catA1) while plasmid pMGRU25-002 had 20 resistant genes [strAB, aadA1,aac(6')-Ib, aac(3)-IId,sul1,2, blaTEM-1A,1B,blaOXA-9, blaCTX-M-15,blaSHV-11, cmlA1, erm(B),mph(A)]. A mutation in the porin OmpK36 was identified which is likely to be associated with the intermediate resistance to carbapenems in the absence of carbapenemase genes. U25 is one of the few K. pneumoniaestrains to harbour clustered regularly interspaced short palindromic repeats (CRISPR) systems. Two CRISPR arrays corresponding to Cas3 family helicase were identified in the genome. When compared to K. pneumoniaeNTUHK2044, a transposase gene InsH of IS5-13 was found inserted.
authors.affiliation: Department of Biotechnology, Dr MGR Educational and Research Institute, Chennai, Tamil Nadu, India.
authors.forename: Zumaana
authors.initials: Z
authors.lastname: Rafiq
authors.affiliation: Department of Biotechnology, Dr MGR Educational and Research Institute, Chennai, Tamil Nadu, India.
authors.forename: Nithin
authors.initials: N
authors.lastname: Sam
authors.affiliation: Department of Biotechnology, Dr MGR Educational and Research Institute, Chennai, Tamil Nadu, India.
authors.forename: Rama
authors.initials: R
authors.lastname: Vaidyanathan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D030541:Databases, Genetic; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D007194:India; D007710:Klebsiella Infections / Q000652:urine*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000235:genetics* / Q000502:physiology; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase