title: Genotypic and antimicrobial characterization of pathogenic bacteria at different stages of cattle slaughtering in southern Brazil.
abstract: Meat can be contaminated in different stages of the slaughtering process and the identification of these stages is the starting point to implement adequate control measures. The objectives of this study were to assess the presence of pathogenic microorganisms in cattle carcasses, to identify the most important contamination points of the slaughtering process, and to evaluate the possible risk factors related to them in a cattle slaughterhouse. To this aim, 108 cattle carcasses were sampled at three stages of the slaughtering process: Point 1 (hides after bleeding); Point 2 (carcasses after hide removal); and Point 3 (carcasses immediately after division). Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Livingstone were isolated from the carcasses. Phenotypic and genotypic characterization indicated that there was cross-contamination among animals, since bacteria with identical genotypic and phenotypic profiles were isolated from different animals at the same sampling day. Furthermore, this is the first report about the isolation of E. coli O157:H7 in a bovine slaughterhouse from southern Brazil.
authors.affiliation: Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul - ICTA/UFRGS, Porto Alegre, Rio Grande do Sul, Brazil; Laboratório de Virologia, Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, Rio Grande do Sul, Brazil. Electronic address: marcialoiko@gmail.com.
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Loiko
authors.affiliation: Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul - ICTA/UFRGS, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Cheila M D
authors.initials: CM
authors.lastname: de Paula
authors.affiliation: Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul - ICTA/UFRGS, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Ana C J
authors.initials: AC
authors.lastname: Langone
authors.affiliation: Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul - ICTA/UFRGS, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Rochele Q
authors.initials: RQ
authors.lastname: Rodrigues
authors.affiliation: Laboratório de Virologia, Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, Rio Grande do Sul, Brazil.
authors.forename: Samuel
authors.initials: S
authors.lastname: Cibulski
authors.affiliation: Laboratório de Leptospirose, Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, Rio Grande do Sul, Brazil.
authors.forename: Rogério de O
authors.initials: Rde O
authors.lastname: Rodrigues
authors.affiliation: Universidade Federal de Viçosa, Centro de Ciências Biológicas e da Saúde, Departamento de Veterinária, Brazil.
authors.forename: Anderson C
authors.initials: AC
authors.lastname: Camargo
authors.affiliation: Universidade Federal de Viçosa, Centro de Ciências Biológicas e da Saúde, Departamento de Veterinária, Brazil.
authors.forename: Luís A
authors.initials: LA
authors.lastname: Nero
authors.affiliation: Laboratório de Biologia Molecular, Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, Rio Grande do Sul, Brazil.
authors.forename: Fabiana Q
authors.initials: FQ
authors.lastname: Mayer
authors.affiliation: Laboratório de Microbiologia e Controle de Alimentos, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul - ICTA/UFRGS, Porto Alegre, Rio Grande do Sul, Brazil.
authors.forename: Eduardo C
authors.initials: EC
authors.lastname: Tondo
mesh_terms: D000003:Abattoirs; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D002417:Cattle / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D004866:Equipment Contamination; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D005516:Food Microbiology; D005838:Genotype; D008089:Listeria monocytogenes / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008460:Meat / Q000382:microbiology*; D012475:Salmonella / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Cattle contamination; Escherichia coli O157:H7; Foodborne pathogens; Listeria monocytogenes; Multidrug resistance; Salmonella spp.