title: Haspin has Multiple Functions in the Plant Cell Division Regulatory Network.
abstract: Progression of cell division is controlled by various mitotic kinases. In animal cells, phosphorylation of histone H3 at Thr3 by the kinase Haspin (haploid germ cell-specific nuclear protein kinase) promotes centromeric Aurora B localization to regulate chromosome segregation. However, less is known about the function of Haspin in regulatory networks in plant cells. Here, we show that inhibition of Haspin with 5-iodotubercidin (5-ITu) in Bright Yellow-2 (BY-2) cells delayed chromosome alignment. Haspin inhibition also prevented the centromeric localization of Aurora3 kinase (AUR3) and disrupted its function. This suggested that Haspin plays a role in the specific positioning of AUR3 on chromosomes in plant cells, a function conserved in animals. The results also indicated that Haspin and AUR3 are involved in the same pathway, which regulates chromosome alignment during prometaphase/metaphase. Remarkably, Haspin inhibition by 5-ITu also led to a severe cytokinesis defect, resulting in binuclear cells with a partially formed cell plate. The 5-ITu treatment did not affect microtubules, AUR1/2 or the NACK-PQR pathway; however, it did alter the distribution of actin filaments on the cell plate. Together, these results suggested that Haspin has several functions in regulating cell division in plant cells: in the localization of AUR3 on centromeres and in regulating late cell plate expansion during cytokinesis.
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
authors.forename: Elena
authors.initials: E
authors.lastname: Kozgunova
authors.affiliation: College of Bioscience and Biotechnology, Chubu University, Matsumoto-cho, Kasugai, Aichi, 478-8501 Japan Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Division of Biological Science, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan JST, CREST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ito
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higashiyama
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602 Japan kuri@bio.nagoya-u.ac.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kurihara
mesh_terms: D064127:Aurora Kinases / Q000378:metabolism; D002455:Cell Division; D002503:Centromere / Q000378:metabolism; D032461:Chromosomes, Plant; D053858:Metabolic Networks and Pathways; D008677:Metaphase; D059828:Plant Cells / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects; D014372:Tubercidin / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D010940:Plant Proteins; C034214:5-iodotubercidin; D011494:Protein Kinases; D064127:Aurora Kinases; D014372:Tubercidin
keywords: Aurora3 kinase; BY-2; Chromosome alignment; Cytokinesis; Haspin