title: [Development of human embryonic stem cell platforms for human health-safety evaluation].
abstract: The human embryonic stem cells (hESCs) serve as a self-renewable, genetically-healthy, pluripotent and single source of all body cells, tissues and organs. Therefore, it is considered as the good standard for all human stem cells by US, Europe and international authorities. In this study, the standard and healthy human mesenchymal progenitors, ligament tissues, cardiomyocytes, keratinocytes, primary neurons, fibroblasts, and salivary serous cells were differentiated from hESCs. The human cellular health-safety of NaF, retinoic acid, 5-fluorouracil, dexamethasone, penicillin G, adriamycin, lead acetate PbAc, bisphenol A-biglycidyl methacrylate (Bis-GMA) were evaluated selectively on the standardized platforms of hESCs, hESCs-derived cardiomyocytes, keratinocytes, primary neurons, and fibroblasts. The evaluations were compared with those on the currently most adopted cellular platforms. Particularly, the sensitivity difference of PM2.5 toxicity on standardized and healthy hESCs derived fibroblasts, currently adopted immortalized human bronchial epithelial cells Beas-2B and human umbilical vein endothelial cells (HUVECs) were evaluated. The RESULTS showed that the standardized hESCs cellular platforms provided more sensitivity and accuracy for human cellular health-safety evaluation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Guang-yan
authors.initials: GY
authors.lastname: Yu
authors.affiliation: Faculty of Dentistry Research Laboratories,National University of Singapore, Singapore 119083,Singapore.
authors.forename: Tong
authors.initials: T
authors.lastname: Cao
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Zou
authors.affiliation: Department of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xue-hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100144, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Shuang-qing
authors.initials: SQ
authors.lastname: Peng
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xu-liang
authors.initials: XL
authors.lastname: Deng
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Sheng-lin
authors.initials: SL
authors.lastname: Li
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: He
authors.initials: H
authors.lastname: Liu
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Science, Beijing 100144, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Ouyang
authors.affiliation: Third Clinical Division, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Centre for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Zeng-ming
authors.initials: ZM
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Evaluation and Research Center for Toxicology, Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Hai-qin
authors.initials: HQ
authors.lastname: Fang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Yu-lan
authors.initials: YL
authors.lastname: Ren
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Ming-ming
authors.initials: MM
authors.lastname: Xu
mesh_terms: D002454:Cell Differentiation; D005347:Fibroblasts / Q000166:cytology; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D032383:Myocytes, Cardiac / Q000166:cytology; D009474:Neurons / Q000166:cytology; D018675:Toxicity Tests