title: Enhanced production of tetramethylpyrazine in Bacillus licheniformis BL1 by bdhA disruption and 2,3-butanediol supplementation.
abstract: The 2,3-butanediol (2,3-BD) dehydrogenase gene (bdhA) of Bacillus licheniformis BL1 was disrupted to construct the tetramethylpyrazine (TMP)-producing BLA strain. During microaerobic fermentation, the bdhA-disrupted BLA strain produced 46.98 g TMP/l, and this yield was 23.99% higher than that produced by the parent BL1 strain. In addition, the yield of acetoin, which is a TMP precursor, also increased by 28.98% in BLA. The TMP production by BL1 was enhanced by supplementing the fermentation medium with 2,3-BD. The yield of TMP improved from 37.89 to 44.77 g/l as the concentration of 2,3-BD increased from 0 to 2 g/l. The maximum TMP and acetoin yields increased by 18.16 and 17.87%, respectively with the increase in 2,3-BD concentration from 0 to 2 g/l. However, no increase was observed when the concentration of 2,3-BD in the matrix was ≥3 g/l. This study provides a valuable strategy to enhance TMP and acetoin productivity of mutagenic strains by gene manipulation and optimizing fermentation conditions.
authors.affiliation: Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology, Jinan, 250353, Shandong, China.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Meng
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, TEDA, Tianjin, 300457, China. xiaodongguang123@163.com.
authors.forename: Dongguang
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology, Jinan, 250353, Shandong, China. ruiming3k@163.com.
authors.forename: Ruiming
authors.initials: R
authors.lastname: Wang
mesh_terms: D000093:Acetoin / Q000378:metabolism; D000332:Aerobiosis; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001407:Bacillus / Q000235:genetics* / Q000378:metabolism*; D002072:Butylene Glycols / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D017353:Gene Deletion; D060847:Metabolic Engineering / Q000379:methods*; D011719:Pyrazines / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D003470:Culture Media; D011719:Pyrazines; C026978:2,3-butylene glycol; D000093:Acetoin; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; C017953:tetramethylpyrazine
keywords: Bacillus licheniformis; Metabolic engineering; TMP; Yield; bdhA gene