title: Anti-Pseudomonas aeruginosa IgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils.
abstract: Moderation of polymorphonuclear neutrophils (PMNs) as part of a critical defense against invading pathogens may offer a promising therapeutic approach to supplement the antibiotic eradication of Pseudomonas aeruginosa infection in non-chronically infected cystic fibrosis (CF) patients. We have observed that egg yolk antibodies (IgY) harvested from White leghorn chickens that target P. aeruginosa opsonize the pathogen and enhance the PMN-mediated respiratory burst and subsequent bacterial killing in vitro. The effects on PMN phagocytic activity were observed in different Pseudomonas aeruginosa strains, including clinical isolates from non-chronically infected CF patients. Thus, oral prophylaxis with anti-Pseudomonas aeruginosa IgY may boost the innate immunity against Pseudomonas aeruginosa in the CF setting by facilitating a rapid and prompt bacterial clearance by PMNs.
authors.affiliation: a Department of Clinical Microbiology , Rigshospitalet, Copenhagen University Hospital , Copenhagen , Denmark.
authors.forename: Kim
authors.initials: K
authors.lastname: Thomsen
authors.affiliation: a Department of Clinical Microbiology , Rigshospitalet, Copenhagen University Hospital , Copenhagen , Denmark.
authors.forename: Lars
authors.initials: L
authors.lastname: Christophersen
authors.affiliation: a Department of Clinical Microbiology , Rigshospitalet, Copenhagen University Hospital , Copenhagen , Denmark.
authors.forename: Peter Østrup
authors.initials: PØ
authors.lastname: Jensen
authors.affiliation: a Department of Clinical Microbiology , Rigshospitalet, Copenhagen University Hospital , Copenhagen , Denmark.|b Department of Immunology and Microbiology , Faculty of Health Sciences University of Copenhagen , Copenhagen , Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bjarnsholt
authors.affiliation: a Department of Clinical Microbiology , Rigshospitalet, Copenhagen University Hospital , Copenhagen , Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Moser
authors.affiliation: a Department of Clinical Microbiology , Rigshospitalet, Copenhagen University Hospital , Copenhagen , Denmark.|b Department of Immunology and Microbiology , Faculty of Health Sciences University of Copenhagen , Copenhagen , Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Høiby
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D002645:Chickens; D064368:Healthy Volunteers; D006801:Humans; D007116:Immunization, Passive / Q000379:methods; D007136:Immunoglobulins / Q000008:administration & dosage / Q000276:immunology*; D050296:Microbial Viability / Q000187:drug effects; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins / Q000008:administration & dosage / Q000276:immunology*; D010587:Phagocytosis; D011552:Pseudomonas Infections / Q000628:therapy; D011550:Pseudomonas aeruginosa / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; C016512:IgY; D007136:Immunoglobulins; D009895:Opsonin Proteins
keywords: IgY; cystic fibrosis; phagocytosis; pseudomonas aeruginosa