title: Testicular Busulfan Injection in Mice to Prepare Recipients for Spermatogonial Stem Cell Transplantation Is Safe and Non-Toxic.
abstract: Current methods of administering busulfan to remove the endogenous germ cells cause hematopoietic toxicity, require special instruments and a narrow transplantation time. We use a direct testicular injection of busulfan method for preparing recipients for SSC transplantation. Male ICR mice (recipients) were divided into four groups, and two experimental groups were treated with a bilateral testicular injection of 4 or 6 mg/kg/side busulfan (n = 60 per concentration group). Mice received an intraperitoneal injection (i.p.) of 40 mg/kg busulfan (n = 60, positive control) and bilateral testicular injections of 50% DMSO (n = 60, negative control). Donor SSCs from RFP-transgenic C57BL/6J mice were introduced into the seminiferous tubules of each recipient testis via efferent duct injection on day 16-17 after busulfan treatment. Recipient mice mated with mature female ICR mice and the number of progeny was recorded. The index detected at day 14, 21, 28, 35 and 70 after busulfan treatment. Blood analysis shows that the toxicity of busulfan treated groups was much lower than i.p. injection groups. Fertility was restored in mice treated with busulfan and donor-derived offspring were obtained after SSC transplantation. Our study indicated that intratesticular injection busulfan for the preparation of recipients in mice is safe and feasible.
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: YuSheng
authors.initials: Y
authors.lastname: Qin
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: YaNan
authors.initials: Y
authors.lastname: He
authors.affiliation: Guangxi University, Nanning, 530004, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Jilin Agriculture University, Changchun, 130118, China.
authors.forename: MingYuan
authors.initials: M
authors.lastname: Liang
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: XiaoLi
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: HaiSheng
authors.initials: H
authors.lastname: Hao
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Qin
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: XueMing
authors.initials: X
authors.lastname: Zhao
authors.affiliation: The Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing, 100193, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D002066:Busulfan / Q000008:administration & dosage* / Q000627:therapeutic use; D005260:Female; D005298:Fertility; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D007248:Infertility, Male / Q000628:therapy*; D007267:Injections; D008297:Male; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D012671:Seminiferous Tubules / Q000166:cytology / Q000648:ultrastructure; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology / Q000637:transplantation*; D033581:Stem Cell Transplantation / Q000379:methods*; D013737:Testis / Q000187:drug effects / Q000502:physiology
chemical_list: D007166:Immunosuppressive Agents; D002066:Busulfan