title: Interactome Mapping Reveals the Evolutionary History of the Nuclear Pore Complex.
abstract: The nuclear pore complex (NPC) is responsible for nucleocytoplasmic transport and constitutes a hub for control of gene expression. The components of NPCs from several eukaryotic lineages have been determined, but only the yeast and vertebrate NPCs have been extensively characterized at the quaternary level. Significantly, recent evidence indicates that compositional similarity does not necessarily correspond to homologous architecture between NPCs from different taxa. To address this, we describe the interactome of the trypanosome NPC, a representative, highly divergent eukaryote. We identify numerous new NPC components and report an exhaustive interactome, allowing assignment of trypanosome nucleoporins to discrete NPC substructures. Remarkably, despite retaining similar protein composition, there are exceptional architectural dissimilarities between opisthokont (yeast and vertebrates) and excavate (trypanosomes) NPCs. Whilst elements of the inner core are conserved, numerous peripheral structures are highly divergent, perhaps reflecting requirements to interface with divergent nuclear and cytoplasmic functions. Moreover, the trypanosome NPC has almost complete nucleocytoplasmic symmetry, in contrast to the opisthokont NPC; this may reflect divergence in RNA export processes at the NPC cytoplasmic face, as we find evidence supporting Ran-dependent mRNA export in trypanosomes, similar to protein transport. We propose a model of stepwise acquisition of nucleocytoplasmic mechanistic complexity and demonstrate that detailed dissection of macromolecular complexes provides fuller understanding of evolutionary processes.
authors.affiliation: The Rockefeller University, New York, New York, United States of America.
authors.forename: Samson O
authors.initials: SO
authors.lastname: Obado
authors.affiliation: The Rockefeller University, New York, New York, United States of America.
authors.forename: Marc
authors.initials: M
authors.lastname: Brillantes
authors.affiliation: The Rockefeller University, New York, New York, United States of America.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Uryu
authors.affiliation: The Rockefeller University, New York, New York, United States of America.
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The Rockefeller University, New York, New York, United States of America.
authors.forename: Natalia E
authors.initials: NE
authors.lastname: Ketaren
authors.affiliation: The Rockefeller University, New York, New York, United States of America.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Chait
authors.affiliation: School of Life Sciences, University of Dundee, Dundee, Scotland, United Kingdom.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Field
authors.affiliation: The Rockefeller University, New York, New York, United States of America.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Rout
mesh_terms: D019143:Evolution, Molecular; D016253:Microscopy, Immunoelectron; D022022:Nuclear Pore / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism*; D020836:Protein Structure, Quaternary; D014346:Trypanosoma brucei brucei
chemical_list: D028861:Nuclear Pore Complex Proteins