title: Inhibition of acute lung injury by rubriflordilactone in LPS-induced rat model through suppression of inflammatory factor expression.
abstract: The present study demonstrates the effect of rubriflordilactone on lipopolysaccharide (LPS)-induced acute kidney injury in rats and MLE-15 cells. LPS administration in rats resulted in formation of edema which was inhibited by pretreatment with rubriflordilactone. The pulmonary tissues of LPS administered rats and MLE-15 cells showed a significant increase in the expression of matrix metalloproteinase-9, interleukin-6 and inducible nitric oxide synthase. However, rubriflordilactone treatment prior to LPS administration caused a significant reduction in the expression of these factors at a concentration of 10 nm/kg. Analysis of the Sirtuin 1 (Sirt1) expression revealed significant (P=0.002) reduction on exposure to LPS in MLE-15 cells. However, rubriflordilactone treatment at 10 nm/ml concentration before LPS exposure caused inhibition of LPS induced reduction in Sirt1 expression. Silencing of Sirt1 by siRNA in MLE-15 cells led to inhibition of increased Sirt1 expression by rubriflordilactone in LPS administered rats. These findings suggest that rubriflordilactone inhibits LPS induced acute lung injury in rats and MLE-15 cells through promotion of Sirt1 expression.
authors.affiliation: Department of Surgical, The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000, P.R. China; Zhumadian City Central HospitalZhumadian 463000, P.R. China.
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Surgical, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450000, P.R. China.
authors.forename: Xin-Guang
authors.initials: XG
authors.lastname: Qiu
authors.affiliation: Department of Surgical, Zhumadian City Central Hospital Zhumadian 463000, P.R. China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Ren
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D011654:Pulmonary Edema / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D034622:RNA Interference; D017208:Rats, Wistar; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014315:Triterpenes; C577686:interleukin-6, mouse; C514794:rubriflordilactone A; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
keywords: Edema; inflammation; interleukin; lung injury; silencer RNA