title: A novel transcription factor gene FHS1 is involved in the DNA damage response in Fusarium graminearum.
abstract: Cell cycle regulation and the maintenance of genome integrity are crucial for the development and virulence of the pathogenic plant fungus Fusarium graminearum. To identify transcription factors (TFs) related to these processes, four DNA-damaging agents were applied to screen a F. graminearum TF mutant library. Sixteen TFs were identified to be likely involved in DNA damage responses. Fhs1 is a fungal specific Zn(II)2Cys6 TF that localises exclusively to nuclei. fhs1 deletion mutants were hypersensitive to hydroxyurea and defective in mitotic cell division. Moreover, deletion of FHS1 resulted in defects in perithecia production and virulence and led to the accumulation of DNA damage. Our genetic evidence demonstrated that the FHS1-associated signalling pathway for DNA damage response is independent of the ATM or ATR pathways. This study identified sixteen genes involved in the DNA damage response and is the first to characterise the novel transcription factor gene FHS1, which is involved in the DNA damage response. The results provide new insights into mechanisms underlying DNA damage responses in fungi, including F. graminearum.
authors.affiliation: Department of Agricultural Biotechnology and Centre for Fungal Pathogenesis, Seoul National University, Seoul 08826, Republic of Korea.|Centre for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Hokyoung
authors.initials: H
authors.lastname: Son
authors.affiliation: Department of Agricultural Biotechnology and Centre for Fungal Pathogenesis, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Minmin
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Agricultural Biotechnology and Centre for Fungal Pathogenesis, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Yoonji
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology and Centre for Fungal Pathogenesis, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Jae Yun
authors.initials: JY
authors.lastname: Lim
authors.affiliation: Department of Agricultural Biotechnology and Centre for Fungal Pathogenesis, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Kyunghun
authors.initials: K
authors.lastname: Min
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Jin-Cheol
authors.initials: JC
authors.lastname: Kim
authors.affiliation: Eco-friendly New Materials Research Group, Research Centre for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
authors.forename: Gyung Ja
authors.initials: GJ
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology and Centre for Fungal Pathogenesis, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Yin-Won
authors.initials: YW
authors.lastname: Lee
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D004249:DNA Damage; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006918:Hydroxyurea / Q000009:adverse effects*; D009154:Mutation; D013172:Spores, Fungal / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D014157:Transcription Factors; D006918:Hydroxyurea