title: Genomic Characterization of a Novel HIV-1 Second-Generation Recombinant Form Originated from CRF01_AE and CRF08_BC in Dali Prefecture of Yunnan Province, China.
abstract: Yunnan seems to be a "hot spot" region of HIV-1 recombination. CRF01_AE and subtype CRF08_BC are two main HIV-1 clades circulating in Yunnan. We report here a novel HIV-1 second-generation recombinant form originated from CRF01_AE and CRF08_BC. The strain (12YN10551) was isolated from a HIV-positive male infected through heterosexual contact in Dali prefecture of Yunnan province, China. This is the first report of HIV-1 near full-length genomic sequence in Dali. Recombinant analysis shows that 12YN10551 was composed of two well-established circulating recombinant forms (CRF01_AE and CRF08_BC). Two CRF01_AE recombinant fragments were inserted into the CRF08_BC backbone genome in the pol/vif/vpr/tat/rev and nef gene regions, respectively. The discovery and characterization of this new recombinant indicate that intersubtype recombination is continuously generating new forms of HIV-1. More work is needed to better monitor the genetic diversity of HIV-1 in this region.
authors.affiliation: 1 Yunnan Provincial Hospital of Infectious Disease, AIDS Care Center (YNACC) , Kunming, China .
authors.forename: Jianjian
authors.initials: J
authors.lastname: Li
authors.affiliation: 2 Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology , Beijing, China .
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: 1 Yunnan Provincial Hospital of Infectious Disease, AIDS Care Center (YNACC) , Kunming, China .
authors.forename: Huiqin
authors.initials: H
authors.lastname: Li
authors.affiliation: 2 Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology , Beijing, China .
authors.forename: Jingyun
authors.initials: J
authors.lastname: Li
authors.affiliation: 1 Yunnan Provincial Hospital of Infectious Disease, AIDS Care Center (YNACC) , Kunming, China .
authors.forename: Shaomin
authors.initials: S
authors.lastname: Yang
authors.affiliation: 1 Yunnan Provincial Hospital of Infectious Disease, AIDS Care Center (YNACC) , Kunming, China .
authors.forename: Mi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: 3 First People's Hospital of Yunnan Province, Kunming University of Science and Technology Affiliated Kun Hua Hospital , Kunming, China .
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D002681:China; D005260:Female; D016679:Genome, Viral; D023281:Genomics; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020010:Heterosexuality; D006801:Humans; D008297:Male; D010802:Phylogeny; D011995:Recombination, Genetic