title: Effects of different amounts and types of dietary fatty acids on the body weight, fat accumulation, and lipid metabolism in hamsters.
abstract: OBJECTIVE
  The aim of this study was to explore the effects of different amounts of dietary fatty acids on body weight, fat accumulation, and lipid metabolism of hamsters.
  
  
  METHODS
  Sixty male golden Syrian hamsters were randomly divided into six groups. Three of the groups (the S groups) were fed experimental diets containing 5%, 15%, and 20% (w/w) fat of soybean oil (S5, S15, and S20, respectively), and the other three groups (the M groups) were fed the same proportions of an experimental oil mixture (M5, M15, and M20, respectively). The experimental oil mixture consisted of 60% monounsaturated fatty acids (MUFAs) and a polyunsaturated-to-saturated fatty acid ratio of 5 with a mixture of soybean and canola oils. Food consumption was measured daily, and body weights were measured weekly. Serum insulin and leptin concentrations were measured and hepatic fatty acid metabolic enzymes and adipose differentiation markers were determined using an enzyme activity analysis and quantitative polymerase chain reaction.
  
  
  RESULTS
  Results showed that the weight and weight gain of the S20 group were significantly greater than those of the other five groups. When the total fat consumption increased, the body weight, weight gain, and adipose tissue weight of the S groups significantly increased, but there were no significant differences in these parameters among the M groups. Serum low-density lipoprotein cholesterol concentrations were significantly lower in the M15 and S15 groups. The S20 group had significantly higher leptin and insulin concentrations and lipoprotein lipase was promoted, but the acetyl-coenzyme A oxidase and carnitine palmitoyltransferase-1, were significantly lower.
  
  
  CONCLUSIONS
  The study demonstrated that a special experimental oil mixture (with 60% MUFAs and a ratio of 5) with high fat can prevent body weight gain and body fat accumulation by lowering insulin concentrations and increasing hepatic lipolytic enzyme activities.
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Yang
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China.
authors.forename: Jung-Su
authors.initials: JS
authors.lastname: Chang
authors.affiliation: Department of Pathology, Taipei Medical University and Wan Fang Hospital, Taipei, Taiwan, Republic of China.
authors.forename: Chi-Long
authors.initials: CL
authors.lastname: Chen
authors.affiliation: Department of Food and Nutrition, Chinese Culture University, Taipei, Taiwan, Republic of China.
authors.forename: Chiu-Li
authors.initials: CL
authors.lastname: Yeh
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China. Electronic address: ychien@tmu.edu.tw.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chien
mesh_terms: D050156:Adipogenesis; D050154:Adiposity; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D008078:Cholesterol, LDL / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000009:adverse effects* / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism / Q000627:therapeutic use*; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism / Q000627:therapeutic use; D015971:Gene Expression Regulation, Enzymologic; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D050177:Overweight / Q000097:blood / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D011897:Random Allocation; D000074262:Rapeseed Oil; D013024:Soybean Oil / Q000008:administration & dosage / Q000009:adverse effects / Q000378:metabolism; D015430:Weight Gain
chemical_list: D015415:Biomarkers; D008078:Cholesterol, LDL; D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D007328:Insulin; D020738:Leptin; D000074262:Rapeseed Oil; D013024:Soybean Oil
keywords: Body fat accumulation; Monounsaturated fatty acids; Polyunsaturated fatty acids; Saturated fatty acids; Total fat consumption