title: TDM1 Regulation Determines the Number of Meiotic Divisions.
abstract: Cell cycle control must be modified at meiosis to allow two divisions to follow a single round of DNA replication, resulting in ploidy reduction. The mechanisms that ensure meiosis termination at the end of the second and not at the end of first division are poorly understood. We show here that Arabidopsis thaliana TDM1, which has been previously shown to be essential for meiotic termination, interacts directly with the Anaphase-Promoting Complex. Further, mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17) dominantly provoked premature meiosis termination after the first division, and the production of diploid spores and gametes. The CDKA;1-CYCA1.2/TAM complex, which is required to prevent premature meiotic exit, phosphorylated TDM1 at T16 in vitro. Finally, while CYCA1;2/TAM was previously shown to be expressed only at meiosis I, TDM1 is present throughout meiosis. These data, together with epistasis analysis, lead us to propose that TDM1 is an APC/C component whose function is to ensure meiosis termination at the end of meiosis II, and whose activity is inhibited at meiosis I by CDKA;1-TAM-mediated phosphorylation to prevent premature meiotic exit. This provides a molecular mechanism for the differential decision of performing an additional round of division, or not, at the end of meiosis I and II, respectively.
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Marta
authors.initials: M
authors.lastname: Cifuentes
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Jolivet
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Cromer
authors.affiliation: RIKEN Center for Sustainable Resource Science, Suehiro, Tsurumi, Yokohama, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Harashima
authors.affiliation: Gregor Mendel Institute, Austrian Academy of Sciences, Vienna, Austria.
authors.forename: Petra
authors.initials: P
authors.lastname: Bulankova
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Renne
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Wayne
authors.initials: W
authors.lastname: Crismani
authors.affiliation: RIKEN Center for Sustainable Resource Science, Suehiro, Tsurumi, Yokohama, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nomura
authors.affiliation: RIKEN Center for Sustainable Resource Science, Suehiro, Tsurumi, Yokohama, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nakagami
authors.affiliation: RIKEN Center for Sustainable Resource Science, Suehiro, Tsurumi, Yokohama, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: University of Hamburg, Biozentrum Klein Flottbek, Department of Developmental Biology, Hamburg, Germany.
authors.forename: Arp
authors.initials: A
authors.lastname: Schnittger
authors.affiliation: Gregor Mendel Institute, Austrian Academy of Sciences, Vienna, Austria.|Central European Institute of Technology (CEITEC), Masaryk University, Kamenice, Brno, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Riha
authors.affiliation: Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Raphael
authors.initials: R
authors.lastname: Mercier
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D032461:Chromosomes, Plant / Q000235:genetics; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D004843:Epistasis, Genetic; D005799:Genes, Dominant; D005820:Genetic Testing; D008540:Meiosis; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D010769:Phosphothreonine / Q000378:metabolism; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D057891:Tetraploidy; D014404:Tubulin / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D016213:Cyclins; D021122:Protein Subunits; C557479:TDM1 protein, Arabidopsis; D014404:Tubulin; D010769:Phosphothreonine; D064173:Anaphase-Promoting Complex-Cyclosome