title: Magnetophoretic-based microfluidic device for DNA Concentration.
abstract: Nucleic acids serve as biomarkers of disease and it is highly desirable to develop approaches to extract small number of such genomic extracts from human bodily fluids. Magnetic particles-based nucleic acid extraction is widely used for concentration of small amount of samples and is followed by DNA amplification in specific assays. However, approaches to integrate such magnetic particles based capture with micro and nanofluidic based assays are still lacking. In this report, we demonstrate a magnetophoretic-based approach for target-specific DNA extraction and concentration within a microfluidic device. This device features a large chamber for reducing flow velocity and an array of μ-magnets for enhancing magnetic flux density. With this strategy, the device is able to collect up to 95 % of the magnetic particles from the fluidic flow and to concentrate these magnetic particles in a collection region. Then an enzymatic reaction is used to detach the DNA from the magnetic particles within the microfluidic device, making the DNA available for subsequent analysis. Concentrations of over 1000-fold for 90 bp dsDNA molecules is demonstrated. This strategy can bridge the gap between detection of low concentration analytes from clinical samples and a range of micro and nanofluidic sensors and devices including nanopores, nano-cantilevers, and nanowires.
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.|Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.|Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
authors.forename: Sangjo
authors.initials: S
authors.lastname: Shim
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.|Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
authors.forename: Jiwook
authors.initials: J
authors.lastname: Shim
authors.affiliation: Department of Molecular Medicine, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA.|Mayo-Illinois Alliance for Technology Based Healthcare.
authors.forename: William R
authors.initials: WR
authors.lastname: Taylor
authors.affiliation: Department of Molecular Medicine, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA.|Mayo-Illinois Alliance for Technology Based Healthcare.
authors.forename: Farhad
authors.initials: F
authors.lastname: Kosari
authors.affiliation: Department of Molecular Medicine, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA.|Mayo-Illinois Alliance for Technology Based Healthcare.
authors.forename: George
authors.initials: G
authors.lastname: Vasmatzis
authors.affiliation: Department of Molecular Medicine, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA.|Mayo-Illinois Alliance for Technology Based Healthcare.
authors.forename: David A
authors.initials: DA
authors.lastname: Ahlquist
authors.affiliation: Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. rbashir@illinois.edu.|Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. rbashir@illinois.edu.|Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. rbashir@illinois.edu.|Mayo-Illinois Alliance for Technology Based Healthcare, . rbashir@illinois.edu.
authors.forename: Rashid
authors.initials: R
authors.lastname: Bashir
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D056656:Lab-On-A-Chip Devices; D060328:Magnetic Phenomena
chemical_list: D004247:DNA
keywords: DNA concentration; DNA conjugated magnetic particles; Magnetophoresis; Uracil linker; Uracil-specific excision reagent enzyme