title: Comparison of the Effect of Two Purification Methods on the Immunogenicity of Recombinant Outer Membrane Protein H of Pasteurella multocida Serovar A:1.
abstract: Recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain X-73 can be purified using affinity chromatography but this adversely affects its immunogenicity. The current study presents the results from an intervention study comparing the immunogenicity of rOmpH purified using electroelution with rOmpH purified using affinity chromatography and native OmpH purified using electroelution and a nonimmunized control group. Chickens immunized with rOmpH purified using electroelution produced the highest ELISA antibody levels against P. multocida strains. Chickens in each of the 5 treatment groups were split into two subgroups for challenge with two different P. multocida strains. The average number of adhesions to CEF cells was statistically significantly lower in sera from chickens immunized with rOmpH or native OmpH purified using electroelution than in those of the three other treatment groups. The survival amongst chickens immunized with rOmpH or native OmpH purified using electroelution indicated high levels of protection. In contrast, survival probability was zero or low in the groups immunized with rOmpH purified using affinity chromatography and in the nonimmunized group. These findings show that the rOmpH purified using electroelution retains its immunogenicity and stimulates high levels of protection in chickens against P. multocida infection.
authors.affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
authors.forename: Arunee
authors.initials: A
authors.lastname: Thanasarasakulpong
authors.affiliation: Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
authors.forename: Pichayanut
authors.initials: P
authors.lastname: Poolperm
authors.affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
authors.forename: Weerapongse
authors.initials: W
authors.lastname: Tangjitjaroen
authors.affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
authors.forename: Thanya
authors.initials: T
authors.lastname: Varinrak
authors.affiliation: Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
authors.forename: Takuo
authors.initials: T
authors.lastname: Sawada
authors.affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand; Veterinary Epidemiology, Economics and Public Health Group, Royal Veterinary College, London AL9 7TA, UK.
authors.forename: Dirk
authors.initials: D
authors.lastname: Pfeiffer
authors.affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
authors.forename: Nattawooti
authors.initials: N
authors.lastname: Sthitmatee