title: Coenzyme Q10 Exerts Anti-Inflammatory Activity and Induces Treg in Graft Versus Host Disease.
abstract: The objective of this study was to determine whether coenzyme Q10 (CoQ10) can reduce the severity of graft versus host disease (GVHD) by inducing regulatory T cells (Tregs). CoQ10 or vehicle was orally administrated once a day for 22 days to mice with GVHD. We measured the alloresponse of the T cells and the GVHD clinical scores. Real-time polymerase chain reaction was used to examine messenger RNA (mRNA) level. Flow cytometry and enzyme-linked immunosorbent assay were used to evaluate protein expression. CoQ10 reduced the T-cell alloresponse and the expression of interferon (IFN)-γ and interleukin (IL)-17. The severity of GVHD and gene expressions of IL-6 and tumor necrosis factor (TNF)-α decreased with CoQ10 treatment. Furthermore, CoQ10 promoted weight gain and survival in GVHD mice. Flow cytometry revealed that CoQ10 dose dependently induced Treg differentiation, but FK506, an immunosuppressive drug, decreased Treg differentiation dose dependently. In conclusion, CoQ10 downregulates the alloreactivity of T cells and reduces GVHD severity, enhancing the differentiation of Tregs.
authors.affiliation: 1 The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea , Seoul, South Korea .
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: 1 The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea , Seoul, South Korea .|2 Impact Biotech , Seoul, South Korea .
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Park
authors.affiliation: 1 The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea , Seoul, South Korea .
authors.forename: Sung-hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: 1 The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea , Seoul, South Korea .|3 Divison of Rheumatology, Department of Internal Medicine, The Catholic University of Korea , Seoul, South Korea .
authors.forename: Mi-La
authors.initials: ML
authors.lastname: Cho
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D005260:Female; D006086:Graft vs Host Disease / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives*
chemical_list: D000893:Anti-Inflammatory Agents; D020381:Interleukin-17; D015850:Interleukin-6; D014451:Ubiquinone; D007371:Interferon-gamma; C024989:coenzyme Q10
keywords: coenzyme Q10; graft versus host disease; regulatory T cells