title: High expression of VEGF and PI3K in glioma stem cells provides new criteria for the grading of gliomas.
abstract: Glioma is a type of tumor derived from glial cells, which is associated with a high level of incidence and mortality. At present, the generation of a fast and efficient method to evaluate the malignancy grade of glioma is required. Cancer stem cells (CSCs) are currently attracting attention in oncological studies; therefore, the present study aimed to investigate novel biomarkers of glioma CSCs, in order to provide new criteria for the grading of glioma. The mRNA expression levels of CD133, (sex determining region Y)-box 2, nestin, vascular endothelial growth factor (VEGF) and phosphoinositide-3-kinase (PI3K) were detected in 15 human samples of high-malignancy glioma and 12 human samples of low-malignancy gliomain vitro. The mRNA expression levels of VEGF and PI3K were higher in the high-malignancy group, as compared with in the low-malignancy group. In conclusion, the mRNA expression levels of VEGF and PI3K in glioma CSCs may be considered a novel criteria for the grading of glioma.
authors.affiliation: Department of Neurosurgery, First Clinical Hospital of Norman Bethune Medical School, Jilin University, Changchun, Jilin 130021, P.R. China; Department of Neurosurgery, The Affiliated Hospital of Beihua University, Jilin, Jilin 132011, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Surgery, First Clinical Hospital of Norman Bethune Medical School, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Luyao
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Beihua University, Jilin, Jilin 132011, P.R. China.
authors.forename: Weigao
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Pathophysiology, Beihua University, Jilin, Jilin 132013, P.R. China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, First Clinical Hospital of Norman Bethune Medical School, Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Luo
keywords: cancer stem cells; disease grading; glioma