title: Reduced Pancreatic Exocrine Function and Organellar Disarray in a Canine Model of Acute Pancreatitis.
abstract: The aim of the present study was to investigate the pancreatic exocrine function in a canine model and to analyze the changes in organelles of pancreatic acinar cells during the early stage of acute pancreatitis (AP). AP was induced by retrograde injection of 5% sodium taurocholate (0.5 ml/kg) into the main pancreatic duct of dogs. The induction of AP resulted in serum hyperamylasemia and a marked reduction of amylase activity in the pancreatic fluid (PF). The pancreatic exocrine function was markedly decreased in subjects with AP compared with the control group. After the induction of AP, histological examination showed acinar cell edema, cytoplasmic vacuolization, fibroblasts infiltration, and inflammatory cell infiltration in the interstitium. Electron micrographs after the induction of AP revealed that most of the rough endoplasmic reticulum (RER) were dilated and that some of the ribosomes were no longer located on the RER. The mitochondria were swollen, with shortened and broken cristae. The present study demonstrated, in a canine model, a reduced volume of PF secretion with decreased enzyme secretion during the early stage of AP. Injury of mitochondria and dilatation and degranulation of RER may be responsible for the reduced exocrine function in AP. Furthermore, the present model and results may be useful for researching novel therapeutic measures in AP.
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Yongyu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Pravin Avinash
authors.initials: PA
authors.lastname: Bhugul
authors.affiliation: Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Xince
authors.initials: X
authors.lastname: Huang
authors.affiliation: YueQing Affiliated Hospital of Wenzhou Medical University, YueQing People's Hospital, Yueqing, Zhejiang Province, China.
authors.forename: Lewei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Ni
authors.affiliation: Zhejiang Provincial Top Key Discipline in surgery, Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Bicheng
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Ningbo University Medical School, Ningbo, Zhejiang Province, China.
authors.forename: Michael
authors.initials: M
authors.lastname: Hehir
authors.affiliation: Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
authors.forename: Mengtao
authors.initials: M
authors.lastname: Zhou
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000096:biosynthesis / Q000097:blood; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D004195:Disease Models, Animal; D004285:Dogs; D045604:Extracellular Fluid / Q000378:metabolism; D008049:Lipase / Q000096:biosynthesis / Q000097:blood; D015388:Organelles / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D046790:Pancreas, Exocrine / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D010195:Pancreatitis / Q000097:blood / Q000378:metabolism* / Q000473:pathology*
chemical_list: D001639:Bicarbonates; D008049:Lipase; D000681:Amylases