title: An insertion/deletion polymorphism in the interleukin-1A 3'untranslated region confers risk for gastric cancer.
abstract: OBJECTIVE
  The aim of the study was to evaluate whether the insertion/deletion polymorphism (rs3783553) locating in the 3' untranslated region (3'UTRs) of IL-1A was related to the risk of gastric cancer (GC) in a Chinese population and explore the possible molecular mechanism.
  
  
  METHODS
  Genomic DNA was extracted from peripheral venous blood of 519 GC patients and 536 healthy control individuals. The IL-1A rs3783553 polymorphism was genotyped by using a polymerase chain reaction assay. The vectors containing the insertion or deletion allele were constructed, and luciferase assay was used to detect the effect of the polymorphism on the transcriptional activity of IL-1A.
  
  
  RESULTS
  Strong evidence of association was observed between the IL-1A rs3783553 polymorphism and susceptibility to GC in the study. In addition, the `TTCA' insertion allele of rs3783553 disrupts the binding site for miR-122 and miR-378, thereby increasing transcription of IL-1α in vitro.
  
  
  CONCLUSIONS
  These findings suggest that functional polymorphism rs3783553 in IL-1A could contribute to GC susceptibility, possibly or at least partially through affecting the transcriptional activity of IL-1A.
authors.forename: Jinkun
authors.initials: J
authors.lastname: Zhang
authors.forename: Huihai
authors.initials: H
authors.lastname: Shi
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Shudan
authors.initials: S
authors.lastname: Zheng
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Zhou
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D044466:Asian People / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D053582:Interleukin-1alpha / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; C508584:IL1A protein, human; D053582:Interleukin-1alpha; C531907:MIRN122 microRNA, human; C526012:MIRN378 microRNA, human; D035683:MicroRNAs
keywords: Insertion/deletion polymorphism; gastric cancer; interleukin-1A; rs3783553