title: OX130 Monoclonal Antibody Recognizes Human SIRPβ1 but Cross-Reacts on SIRPα from One Allele.
abstract: The SIRP family of myeloid-paired receptors are characterized by having both activating and inhibiting members with extracellular regions that are relatively similar. Making good reagents to these receptors is not straightforward, particularly as they are relatively polymorphic. We describe the production of a monoclonal antibody (MAb) called OX130 that recognizes both common alleles of the human activating SIRPβ1 receptor but also cross-reacts with one of the common alleles of the inhibitory human SIRPα receptor. Thus one might get different outcomes when this MAb is used in assays from different individuals and shows the importance of characterizing SIRP MAb in this way.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford , Oxford, United Kingdom .
authors.forename: Deborah
authors.initials: D
authors.lastname: Hatherley
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford , Oxford, United Kingdom .
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Aknin
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford , Oxford, United Kingdom .
authors.forename: A Neil
authors.initials: AN
authors.lastname: Barclay
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C501921:SIRPB1 protein, human