title: NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption.
abstract: BACKGROUND
  Previous studies have reported that follicle-stimulating hormone (FSH) is often added to culture media to induce oocyte meiotic resumption and maturation and to improve subsequent embryonic development during in vitro maturation (IVM). However, the underlying mechanisms remain unclear.
  
  
  METHODS
  Cumulus-oocyte complexes (COCs) were collected from ovaries 46-48 h after the female mice were intraperitoneally injected with 8 IU equine chorionic gonadotropin (eCG) and then the COCs were cultured in different medium. qRT-PCR analysis was used to assess mRNA expression of EGF-like factors and natriuretic peptide receptor 2 (NPR2). Western Blot analysis was used to assess phosphorylation of mitogen-activated protein kinase 3/1 (MAPK3/1). The oocytes were morphologically assessed for meiotic resumption.
  
  
  RESULTS
  FSH stimulated the expression of EGF-like factors, the activation of MAPK3/1, a decrease in NPR2 mRNA and oocyte meiotic resumption. Moreover, the FSH-induced decrease in NPR2 and oocyte meiotic resumption occurred via the MAPK3/1 singling pathway, which was activated by the epidermal growth factor receptor (EGFR) pathway.
  
  
  CONCLUSIONS
  NPR2 is involved in FSH-mediated oocyte meiotic resumption, and this process is associated with the EGFR and MAPK3/1 signaling pathways.
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China. 530934101@qq.com.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. 530934101@qq.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China. 79493532@qq.com.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. 79493532@qq.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China. 1273314775@qq.com.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. 1273314775@qq.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China. 1289240911@qq.com.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. 1289240911@qq.com.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Xi
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China. 515586982@qq.com.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. 515586982@qq.com.
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China. baohuama@163.com.|College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. baohuama@163.com.
authors.forename: Baohua
authors.initials: B
authors.lastname: Ma
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D002478:Cells, Cultured; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology*; D015870:Gene Expression; D020935:MAP Kinase Signaling System; D008540:Meiosis; D051379:Mice; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009865:Oocytes / Q000502:physiology*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000502:physiology*; D015533:Transcriptional Activation; D000099242:Atrial Natriuretic Factor Receptor B
chemical_list: D066246:ErbB Receptors; D005640:Follicle Stimulating Hormone; D048052:Mitogen-Activated Protein Kinase 3; D012333:RNA, Messenger; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B; C541485:EGFR protein, mouse