title: Anticancer activity of glucomoringin isothiocyanate in human malignant astrocytoma cells.
abstract: Isothiocyanates (ITCs) released from their glucosinolate precursors have been shown to inhibit tumorigenesis and they have received significant attention as potential chemotherapeutic agents against cancer. Astrocytoma grade IV is the most frequent and most malignant primary brain tumor in adults without any curative treatment. New therapeutic drugs are therefore urgently required. In the present study, we investigated the in vitro antitumor activity of the glycosylated isothiocyanate moringin [4-(α-l-rhamnopyranosyloxy)benzyl isothiocyanate] produced from quantitative myrosinase-induced hydrolysis of glucomoringin (GMG) under neutral pH value. We have evaluated the potency of moringin on apoptosis induction and cell death in human astrocytoma grade IV CCF-STTG1 cells. Moringin showed to be effective in inducing apoptosis through p53 and Bax activation and Bcl-2 inhibition. In addition, oxidative stress related Nrf2 transcription factor and its upstream regulator CK2 alpha expressions were modulated at higher doses, which indicated the involvement of oxidative stress-mediated apoptosis induced by moringin. Moreover, significant reduction in 5S rRNA was noticed with moringin treatment. Our in vitro results demonstrated the antitumor efficacy of moringin derived from myrosinase-hydrolysis of GMG in human malignant astrocytoma cells.
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, contrada Casazza, 98124 Messina, Italy.
authors.forename: Thangavelu Soundara
authors.initials: TS
authors.lastname: Rajan
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di Ricerca per le Colture Industriali (CREA-CIN), Via Di Corticella 133, Bologna 40128, Italy.
authors.forename: Gina Rosalinda
authors.initials: GR
authors.lastname: De Nicola
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di Ricerca per le Colture Industriali (CREA-CIN), Via Di Corticella 133, Bologna 40128, Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Iori
authors.affiliation: Université d'Orléans et CNRS, ICOA, UMR 7311, BP 6759, F-45067 Orléans, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Rollin
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, contrada Casazza, 98124 Messina, Italy.
authors.forename: Placido
authors.initials: P
authors.lastname: Bramanti
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, contrada Casazza, 98124 Messina, Italy. Electronic address: emazzon.irccs@gmail.com.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Mazzon
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001254:Astrocytoma / Q000473:pathology*; D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D017879:Isothiocyanates / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D029587:Moringa / Q000737:chemistry*; D018384:Oxidative Stress; D012210:Rhamnose / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C000600309:4-((alpha-L-rhamnosyloxy)benzyl)isothiocyanate; D000972:Antineoplastic Agents, Phytogenic; D017879:Isothiocyanates; D012210:Rhamnose
keywords: Anti-tumor effects; Apoptosis; Glioblastoma multiforme; Glucomoringin; Isothiocyanates; Moringa oleifera; Oxidative stress