title: Silencing of FRAT1 by siRNA inhibits the proliferation of SGC7901 human gastric adenocarcinoma cells.
abstract: Frequently rearranged in advanced T cell lymphomas-1 (FRAT1) positively regulates the Wnt/β-catenin signaling pathway by inhibiting glycogen synthase kinase-3 mediated phosphorylation of β-catenin. FRAT1 is a proto-oncogene, implicated in tumorigenesis. The present study aimed to investigate the effects of FRAT1 silencing on the proliferation and apoptosis of SGC7901 cells. FRAT1 in SGC7901 cells was silenced by RNA interference. Reverse transcription-quantitative polymerase chain reaction was used for the analysis of FRAT1 mRNA and western blotting was used to evaluate FRAT1 and β-catenin protein levels. Cell proliferation was analyzed by the MTT assay. Cell cycle distribution and apoptosis were analyzed by flow cytometry. The expression of FRAT1 mRNA, FRAT1 and β-catenin protein in FRAT1-silenced SGC7901 cells were reduced significantly compared to untreated cells. The proliferation of FRAT1 silenced SGC7901 cells decreased significantly The FRAT1 silenced SGC7901 cells were arrested at G0/G1 stage to a greater degree, and apoptosis was increased. In summary, silencing of FRAT1 inhibits SGC7901 cell proliferation and induces apoptosis, possible through a reduction in β-catenin expression. FRAT1 may serve as a prognostic biomarker and therapeutic target for gastric cancer.
authors.affiliation: Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Qinggong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: L U
authors.initials: LU
authors.lastname: Shang
authors.affiliation: Department of Infectious Diseases, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Zirong
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China.
authors.forename: Dekui
authors.initials: D
authors.lastname: Xu
keywords: RNA interference; SGC7901 cells; apoptosis; frequently rearranged in advanced T cell lymphomas-1; proliferation