title: The use of real-time polymerase chain reaction with high resolution melting (real-time PCR-HRM) analysis for the detection and discrimination of nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus.
abstract: The real-time PCR-HRM analysis was developed for the detection and discrimination of the quarantine nematode Bursaphelenchus xylophilus and Bursaphelenchus mucronatus. A set of primers was designed to target the ITS region of rDNA. The results have demonstrated that this analysis is a valuable tool for differentiation of these both species.
authors.affiliation: Department of Biological Pest Control, Institute of Plant Protection, National Research Institute, Władysława Węgorka 20, 60-318, Poznan, Poland. Electronic address: A.Filipiak@iorpib.poznan.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Filipiak
authors.affiliation: Department of Virology and Bacteriology, Institute of Plant Protection, National Research Institute, Władysława Węgorka 20, 60-318, Poznan, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Hasiów-Jaroszewska
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D017187:Tylenchida / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D021903:DNA, Ribosomal Spacer
keywords: Bursaphelenchus mucronatus; Bursaphelenchus xylophilus; Detection; Pine wilt disease; Quarantine nematode; Real-time PCR-HRM