title: CRMPs colocalize and interact with cytoskeleton in hippocampal neurons.
abstract: CRMP family proteins (CRMPs) are widely expressed in the developing neurons, mediating a variety of fundamental functions such as growth cone guidance, neuronal polarity and axon elongation. However, whether all the CRMP proteins interact with cytoskeleton remains unknown. In this study, we found that in cultured hippocampal neurons, CRMPs mainly colocalized with tubulin and actin network in neurites. In growth cones, CRMPs colocalized with tubulinmainly in the central (C-) domain and transition zone (T-zone), less in the peripheral (P-) domain and colocalized with actin in all the C-domain, T-zone and P-domain. The correlation efficiency of CRMPs between actin was significantly higher than that between tubulin, especially in growth cones. We successfully constructed GST-CRMPs plasmids, expressed and purified the GST-CRMP proteins. By GST-pulldown assay, all the CRMP family proteins were found to beinteracted with cytoskeleton proteins. Taken together, we revealed that CRMPs were colocalized with cytoskeleton in hippocampal neurons, especially in growth cones. CRMPs can interact with both tubulin and actin, thus mediating neuronal development.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Jinan UniversityGuangzhou 510630, China; Department of Anatomy, Medical College of Jinan UniversityGuangzhou 510630, China.
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Anatomy, Medical College of Jinan University Guangzhou 510630, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Jinan UniversityGuangzhou 510630, China; Department of Anatomy, Medical College of Jinan UniversityGuangzhou 510630, China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Medical College of Jinan University Guangzhou 510630, China.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Medical College of Jinan University Guangzhou 510630, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Anatomy, Medical College of Jinan University Guangzhou 510630, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Jinan University Guangzhou 510630, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Lin
keywords: CRMP-5; actin; cytoskeleton; growth cone; hippocampal neuron; neurite growth; tubulin