title: Implantation of muscle satellite cells overexpressing myogenin improves denervated muscle atrophy in rats.
abstract: This study evaluated the effect of muscle satellite cells (MSCs) overexpressing myogenin (MyoG) on denervated muscle atrophy. Rat MSCs were isolated and transfected with the MyoG-EGFP plasmid vector GV143. MyoG-transfected MSCs (MTMs) were transplanted into rat gastrocnemius muscles at 1 week after surgical denervation. Controls included injections of untransfected MSCs or the vehicle only. Muscles were harvested and analyzed at 2, 4, and 24 weeks post-transplantation. Immunofluorescence confirmed MyoG overexpression in MTMs. The muscle wet weight ratio was significantly reduced at 2 weeks after MTM injection (67.17±6.79) compared with muscles injected with MSCs (58.83±5.31) or the vehicle (53.00±7.67; t=2.37, P=0.04 and t=3.39, P=0.007, respectively). The muscle fiber cross-sectional area was also larger at 2 weeks after MTM injection (2.63×10³±0.39×10³) compared with MSC injection (1.99×10³±0.58×10³) or the vehicle only (1.57×10³±0.47×10³; t=2.24, P=0.049 and t=4.22, P=0.002, respectively). At 4 and 24 weeks post-injection, the muscle mass and fiber cross-sectional area were similar across all three experimental groups. Immunohistochemistry showed that the MTM group had larger MyoG-positive fibers. The MTM group (3.18±1.13) also had higher expression of MyoG mRNA than other groups (1.41±0.65 and 1.03±0.19) at 2 weeks after injection (t=2.72, P=0.04). Transplanted MTMs delayed short-term atrophy of denervated muscles. This approach can be optimized as a novel stand-alone therapy or as a bridge to surgical re-innervation of damaged muscles.
authors.affiliation: The First Affiliated Hospital, College of Medicine, Department of Hand Surgery and Microsurgery Center, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Shen
authors.affiliation: The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lv
authors.affiliation: The First Affiliated Hospital, College of Medicine, Department of Hand Surgery and Microsurgery Center, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Shen
authors.affiliation: The First Affiliated Hospital, College of Medicine, Department of Plastic Surgery, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Xu
authors.affiliation: The First Affiliated Hospital, College of Medicine, Department of Hand Surgery and Microsurgery Center, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: H
authors.initials: H
authors.lastname: Lu
authors.affiliation: The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
authors.forename: L C
authors.initials: LC
authors.lastname: Fu
authors.affiliation: College of Medicine, Toxicology Laboratory, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: T
authors.initials: T
authors.lastname: Duan
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D008297:Male; D009121:Muscle Denervation / Q000534:rehabilitation*; D018482:Muscle, Skeletal / Q000294:innervation*; D009133:Muscular Atrophy / Q000209:etiology / Q000534:rehabilitation*; D018008:Myogenin / Q000235:genetics / Q000378:metabolism*; D009929:Organ Size / Q000235:genetics; D010957:Plasmids; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D020426:Sciatic Neuropathy / Q000534:rehabilitation; D014162:Transfection
chemical_list: D018008:Myogenin