title: Effects of down-regulation of clusterin by small interference RNA on human acute myeloid leukemia cells.
abstract: OBJECTIVE
  Up-regulation of clusterin is associated with the survival and progression of various malignancies, and down-regulation of clusterin promotes apoptosis and inhibits invasion. The aim of this study was to explore the effect of clusterin small interference RNA (siRNA) on the proliferation, apoptosis and invasion of HL-60 acute myeloid leukemia (AML) cells.
  
  
  METHODS
  siRNA transfection was performed using Lipofectamine™2000 reagent. Relative protein expressions were quantified by Western blot. Trypan blue assay was performed to assess tumor cell proliferation after siRNA transfection. The cytotoxic effect of clusterin siRNA on leukemic cells was measured using MTT assay. Apoptosis was detected using fluorescence microscopy assay. Migration and invasion was detected after clusterin was silenced.
  
  
  RESULTS
  Clusterin siRNA clearly lowered clusterin protein levels in a time- dependent manner, leading to marked inhibition of cell survival, proliferation and invasion. Furthermore, clusterin down-regulation significantly enhanced the extent of HL-60 apoptotic cells.
  
  
  CONCLUSIONS
  Our results suggest that the down-regulation of clusterin by siRNA can effectively trigger apoptosis and inhibit the proliferation and invasion of leukemic cells. Therefore, clusterin siRNA may be a potent adjuvant in AML therapy.
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Laiwu Laiwu, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, People's Hospital of Laiwu Laiwu, China.
authors.forename: Ruidong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Cardiology, People's Hospital of Zhangqiu Jinan, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology, People's Hospital of Zhangqiu Jinan, China.
authors.forename: Hengjuan
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Clinical Laboratory, Qingdao Women and Children's Hospital Qingdao, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
keywords: Acute myeloid leukemia; apoptosis; clusterin; invasion; small interference RNA