title: Multi-loci diagnosis of acute lymphoblastic leukaemia with high-throughput sequencing and bioinformatics analysis.
abstract: High-throughput sequencing (HTS) is considered a technical revolution that has improved our knowledge of lymphoid and autoimmune diseases, changing our approach to leukaemia both at diagnosis and during follow-up. As part of an immunoglobulin/T cell receptor-based minimal residual disease (MRD) assessment of acute lymphoblastic leukaemia patients, we assessed the performance and feasibility of the replacement of the first steps of the approach based on DNA isolation and Sanger sequencing, using a HTS protocol combined with bioinformatics analysis and visualization using the Vidjil software. We prospectively analysed the diagnostic and relapse samples of 34 paediatric patients, thus identifying 125 leukaemic clones with recombinations on multiple loci (TRG, TRD, IGH and IGK), including Dd2/Dd3 and Intron/KDE rearrangements. Sequencing failures were halved (14% vs. 34%, P = 0.0007), enabling more patients to be monitored. Furthermore, more markers per patient could be monitored, reducing the probability of false negative MRD results. The whole analysis, from sample receipt to clinical validation, was shorter than our current diagnostic protocol, with equal resources. V(D)J recombination was successfully assigned by the software, even for unusual recombinations. This study emphasizes the progress that HTS with adapted bioinformatics tools can bring to the diagnosis of leukaemia patients.
authors.affiliation: Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Ferret
authors.affiliation: Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Caillault
authors.affiliation: Functional and Structural Genomic Platform, Université de Lille, Lille, France.
authors.forename: Shéhérazade
authors.initials: S
authors.lastname: Sebda
authors.affiliation: School of Social and Community Medicine, University of Bristol, Bristol, UK.|SIRIC ONCOLille, CRIStAL, UMR 9189, Université de Lille, Lille, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Duez
authors.affiliation: Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Grardel
authors.affiliation: Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Duployez
authors.affiliation: Functional and Structural Genomic Platform, Université de Lille, Lille, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Villenet
authors.affiliation: Functional and Structural Genomic Platform, Université de Lille, Lille, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Figeac
authors.affiliation: Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire de Lille, Lille, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Preudhomme
authors.affiliation: CRIStAL, UMR 9189, CNRS, Université de Lille, Lille, France.|Inria, Lille, France.
authors.forename: Mikaël
authors.initials: M
authors.lastname: Salson
authors.affiliation: CRIStAL, UMR 9189, CNRS, Université de Lille, Lille, France.|Inria, Lille, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Giraud
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells; D019295:Computational Biology / Q000379:methods*; D003951:Diagnostic Errors / Q000517:prevention & control; D015329:Gene Rearrangement, T-Lymphocyte; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods* / Q000592:standards; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018365:Neoplasm, Residual / Q000175:diagnosis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis*; D011446:Prospective Studies; D012984:Software; D060152:V(D)J Recombination / Q000235:genetics; D055815:Young Adult
keywords: Acute Lymphoblastic Leukaemia (ALL); Bioinformatics; Diagnosis; High-Throughput Sequencing (HTS); Immune Repertoire Sequencing (Rep-Seq)