title: The Mechanism of Adaptation of Breast Cancer Cells to Hypoxia: Role of AMPK/mTOR Signaling Pathway.
abstract: We studied the mechanisms of adaptation of human breast cancer cells MCF-7 to hypoxia and analyzed the role of AMPK/mTOR signaling pathway in the maintenance of cell proliferation under hypoxic conditions. It was found that long-term culturing (30 days or more) of MCF-7 cells under hypoxic conditions induced their partial adaptation to hypoxia. Cell adaptation to hypoxia was associated with attenuation of hypoxia-dependent AMPK induction with simultaneous constitutive activation of mTOR and Akt. These findings suggest that these proteins can be promising targets for targeted therapy of tumors developing under hypoxic conditions.
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Sorokin
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia. alex.scherbakov@gmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Scherbakov
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Yakushina
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: S E
authors.initials: SE
authors.lastname: Semina
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Gudkova
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Krasil'nikov
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000222:Adaptation, Physiological / Q000502:physiology*; D001943:Breast Neoplasms / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases
keywords: AMPK; adaptation to hypoxia; breast cancer; hypoxia; mTOR