title: Near-Full-Length Genome Sequences of a Novel HIV-1 Circulating Recombinant Form, CRF01_AE/B'/C (CRF78_cpx), in Yunnan, China.
abstract: We report a novel HIV circulating recombinant form (CRF78_cpx) composed mainly of CRF01_AE with inserts from subtypes B and C identified from three epidemiologically unlinked individuals in Yunnan province, China. Two of the subjects are heterosexual men and one is a male intravenous drug user. Sequencing and analyzing the near-full-length genome of these three isolates (YNTC88, YNTC19, and YNTC35) revealed identical recombination breakpoints in all three viruses, but considerable genetic diversity between them, across the genomes, indicating that this is not a newly created CRF, only newly detected. CRF78_cpx differs from previously documented CRF01-AE/B'/C forms in its distinct backbone, inserted fragment size, and breakpoints, and is not related to other described recombinants in the region such as CRF07_BC or CRF65_cpx (also composed of CRF01_AE, B', and C). Our present findings further enrich the diversity of the prevalent HIV-1 CRFs in Yunnan, which is considered as an epicenter of HIV-1 infections in China.
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: Yindi
authors.initials: Y
authors.lastname: Song
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Miao
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: Binghui
authors.initials: B
authors.lastname: Wang
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: A-Mei
authors.initials: AM
authors.lastname: Zhang
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Diagnosis, Kunming University of Science and Technology , Kunming, China .
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xia
mesh_terms: D002681:China / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA