title: [Antibodies to mutated citrullinated vimentin and some associated autoantibodies in juvenile idiopathic arthritis].
abstract: OBJECTIVE
  To compare the diagnostic value of antibodies to mutated citrullinated vimentin (MCV) and some associated autoantibodies in juvenile idiopathic arthritis and to further analyze the relation between antibodies and inflammatory markers.
  
  
  METHODS
  Antibodies to cyclic citrullinated peptides (CCP) and anti-MCV antibodies were detected by enzyme-linked immunosorbent assay (ELISA), antiperinuclear factor (APF) and antikeratin antibody (AKA) by indirect immunofluorescent assay, as well as rheumatoid factor (RF) by latex agglutination test in serum samples from 113 patients with JIA and 56 children without rheumatoid arthritis.
  
  
  RESULTS
  (1) The positive rate of anti-MCV antibodies, anti-CCP antibodies, and RF was 16.8%, 14.2%, and 21.2% in the JIA. In the other group, the positive rate was 2.2%, 2.2%, and 6.5%. There was a significant difference between the two groups (χ(2)=8.105, 6.337, 7.036, P<0.05). The positive rate of AKA and APF were not significantly different. The area under the ROC curve of anti-MCV antibodies, anti-CCP antibodies, RF, AKA, APF was 0.579, 0.561, 0.578, 0.539, 0.505. (2) The positive rate of anti-MCV antibodies and anti-CCP antibodies were higher than other antibodies. In the RF-positive polyarticular disease patients, they were higher than those in the other subtypes (P<0.05). Antibody levels were not significantly different (P>0.05) from other subtypes. (3) The swollen joint counts and tender joint counts had a low correlation to anti-MCV antibodies, anti-CCP antibodies, RF, AKA and APF. No correlation was found between ESR, CRP and anti-MCV antibodies, anti-CCP antibodies, RF, AKA and APF.
  
  
  CONCLUSIONS
  The diagnostic value of anti-MCV antibodies is low for JIA. The positive rate of anti-MCV antibodies was higher than the other antibodies in the classification of JIA. There was a low correlation between anti-MCV antibodies, anti-CCP antibodies, RF, AKA, APF and swollen joint counts, tender joint counts.
authors.affiliation: Department of Rheumatology, the No. 264 Hospital, Taiyuan, Shanxi Province 030001, China.
authors.forename: Luyue
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Email: rujinli@163.com.
authors.forename: Jinli
authors.initials: J
authors.lastname: Ru
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Jie
authors.forename: Guozhu
authors.initials: G
authors.lastname: Che
authors.forename: Xueqin
authors.initials: X
authors.lastname: Jin
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
mesh_terms: D000974:Antibodies, Antinuclear / Q000097:blood; D001171:Arthritis, Juvenile / Q000097:blood*; D001172:Arthritis, Rheumatoid; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D010456:Peptides, Cyclic / Q000276:immunology; D012372:ROC Curve; D012217:Rheumatoid Factor / Q000097:blood; D014746:Vimentin / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D015415:Biomarkers; D010456:Peptides, Cyclic; D014746:Vimentin; C030902:antiperinuclear factor; C487763:cyclic citrullinated peptide; D012217:Rheumatoid Factor