title: Antibiotic binding of STY3178, a yfdX protein from Salmonella Typhi.
abstract: The yfdX family proteins are known for long time to occur in various virulent bacteria including their multidrug resistant (MDR) strains, without any direct assigned function for them. However, yfdX protein along with other proteins involved in acid tolerance response is reported to be up regulated by the multidrug response regulatory system in E. coli. Hence, molecular and functional characterization of this protein is important for understanding of key cellular processes in bacterial cells. Here we study STY3178, a yfdX protein from a MDR strain of typhoid fever causing Salmonella Typhi. Our experimental results indicate that STY3178 is a helical protein existing in a trimeric oligomerization state in solution. We also observe many small antibiotics, like ciprofloxacin, rifampin and ampicillin viably interact with this protein. The dissociation constants from the quenching of steady state fluorescence and isothermal titration calorimetry show that ciprofloxacin binding is stronger than rifampin followed by ampicillin.
authors.affiliation: Department of Chemical, Biological And Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt lake, Kolkata 700098, India.
authors.forename: Paramita
authors.initials: P
authors.lastname: Saha
authors.affiliation: Department of Chemical, Biological And Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt lake, Kolkata 700098, India.
authors.forename: Camelia
authors.initials: C
authors.lastname: Manna
authors.affiliation: Division of Clinical Medicine, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM Beleghata, Kolkata 700 010, India.
authors.forename: Santasabuj
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Chemical, Biological And Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt lake, Kolkata 700098, India.
authors.forename: Mahua
authors.initials: M
authors.lastname: Ghosh
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D019906:Nuclear Magnetic Resonance, Biomolecular; D066329:Protein Aggregates; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012485:Salmonella typhi / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D066329:Protein Aggregates; D011994:Recombinant Proteins