title: miR-155 acts as an anti-inflammatory factor in atherosclerosis-associated foam cell formation by repressing calcium-regulated heat stable protein 1.
abstract: Atherosclerosis (AS) is chronic inflammation in response to lipid accumulation. MicroRNA-155 (miR-155) is being increasingly studied to evaluate its potential as diagnostic biomarkers and therapeutic targets in many diseases. However, delineating the role of miR-155 in AS remains difficult. Here, we detected constitutive expression of several microRNAs (miRNAs) possibly associated with cardiovascular disease in foam cells and clinical specimens from patients with AS. Among them, we found that the level of miR-155 in foam cells was the most significantly elevated in a dose- and time-dependent manner. In addition, the expression of miR-155 was elevated in the plasma and plaque of patients with AS. We also reported for the first time that miR-155 targets calcium-regulated heat stable protein 1 (CARHSP1), which regulates the stability of tumor necrosis factor alpha (TNF-α) mRNA. Furthermore, we investigated the mechanism by which the miR-155 level is elevated. miR-155 upregulation is due to transcriptional regulation by nuclear factor (NF)-κB, which is activated by the inflammatory factor TNF-α. In summary, increased miR-155 relieves chronic inflammation by a negative feedback loop and plays a protective role during atherosclerosis-associated foam cell formation by signaling through the miR-155-CARHSP1-TNF-α pathway.
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.|School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Heming
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.|Cancer Research Institute of Wuhan, Wuhan 430014, China.
authors.forename: Shuiyi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Tangwei
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.|Cancer Research Institute of Wuhan, Wuhan 430014, China.|Department of Central Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.|Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Medical Laboratory, Central Hospital of Wuhan, Wuhan 430014, China.|School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China.|Cancer Research Institute of Wuhan, Wuhan 430014, China.
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Lu
mesh_terms: D050197:Atherosclerosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001483:Base Sequence; D001665:Binding Sites; D016022:Case-Control Studies; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D025461:Feedback, Physiological; D005487:Foam Cells / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D058226:Plaque, Atherosclerotic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D061251:Primary Cell Culture; D055550:Protein Stability; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: C114342:CARHSP1 protein, human; D004268:DNA-Binding Proteins; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D010750:Phosphoproteins; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; D008156:Luciferases