title: Structural Basis for the Unique Multivalent Readout of Unmodified H3 Tail by Arabidopsis ORC1b BAH-PHD Cassette.
abstract: DNA replication initiation relies on the formation of the origin recognition complex (ORC). The plant ORC subunit 1 (ORC1) protein possesses a conserved N-terminal BAH domain with an embedded plant-specific PHD finger, whose function may be potentially regulated by an epigenetic mechanism. Here, we report structural and biochemical studies on the Arabidopsis thaliana ORC1b BAH-PHD cassette which specifically recognizes the unmodified H3 tail. The crystal structure of ORC1b BAH-PHD cassette in complex with an H3(1-15) peptide reveals a strict requirement for the unmodified state of R2, T3, and K4 on the H3 tail and a novel multivalent BAH and PHD readout mode for H3 peptide recognition. Such recognition may contribute to epigenetic regulation of the initiation of DNA replication.
authors.affiliation: Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Sisi
authors.initials: S
authors.lastname: Li
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Du
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Alex W
authors.initials: AW
authors.lastname: Wilkinson
authors.affiliation: Department of Biology, Stanford University, Stanford, CA 94305, USA.
authors.forename: Or
authors.initials: O
authors.lastname: Gozani
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Jacobsen
authors.affiliation: Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Dinshaw J
authors.initials: DJ
authors.lastname: Patel
authors.affiliation: Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China. Electronic address: jmdu@sibs.ac.cn.
authors.forename: Jiamu
authors.initials: J
authors.lastname: Du
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D004261:DNA Replication; D044127:Epigenesis, Genetic; D006657:Histones / Q000378:metabolism; D008958:Models, Molecular; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D000072417:Protein Domains
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D006657:Histones; C504721:ORC1b protein, Arabidopsis; D010455:Peptides