title: Influence of enrofloxacin traces in drinking water to doxycycline tissue pharmacokinetics in healthy and infected by Mycoplasma gallisepticum broiler chickens.
abstract: Most of antibiotics, administrated in the treatment of poultry diseases are dissolved in drinking water, and it can lead to water supply systems contamination, especially when the regular cleaning is not using. This situation can lead to unconscious administration of low doses of antibiotics to untreated animals. The aim of this study was to clarify the impact of the exposure of enrofloxacin traces (500 μg l(-1)) to doxycycline pharmacokinetics in healthy and experimentally Mycoplasma gallisepticum infected broiler chickens., Two experimental groups, received of enrofloxacin in water and all groups, received 20 mg kg(-1) bw of doxycycline. The compounds concentrations in muscles and livers were determined by LC-MS/MS. The maximum drug tissue concentration (Cmax) of doxycycline was highest in liver obtained from infected chickens which, received enrofloxacin traces (ENR + DC/MG). It was about 40% higher than in healthy chickens from group I which received only doxycycline. It was found that the concentration-time curve AUC(0-t) values in group ENR + DC/MG were almost 75% higher than in the group (DC) and 35% higher than in group (ENR + DC) which also received enrofloxacin traces. The constant exposure of broiler chickens on enrofloxacin traces as well as infection, may significantly influenced on doxycycline tissue pharmacokinetic profile.
authors.affiliation: Department of Pharmacology and Toxycology, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland. Electronic address: malgorzata.gbylik@piwet.pulawy.pl.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Gbylik-Sikorska
authors.affiliation: Department of Pharmacology and Toxycology, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Posyniak
authors.affiliation: Department of Pharmacology and Toxycology, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sniegocki
authors.affiliation: Department of Pharmacology and Toxycology, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Sell
authors.affiliation: Department of Pharmacology and Toxycology, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Gajda
authors.affiliation: Department of Poultry Diseases, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sawicka
authors.affiliation: Department of Poultry Diseases, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Olszewska-Tomczyk
authors.affiliation: Department of Pharmacology and Toxycology, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bladek
authors.affiliation: Department of Poultry Diseases, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Tomczyk
authors.affiliation: Department of Pharmacology and Toxycology, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Zmudzki
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use; D002645:Chickens; D004318:Doxycycline / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D060766:Drinking Water; D004347:Drug Interactions; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D009175:Mycoplasma Infections / Q000188:drug therapy / Q000662:veterinary*; D045686:Mycoplasma gallisepticum; D011201:Poultry Diseases / Q000188:drug therapy / Q000382:microbiology*; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D060766:Drinking Water; D024841:Fluoroquinolones; D000077422:Enrofloxacin; D004318:Doxycycline
keywords: Broiler chickens; Doxycycline; Drinking water; Enrofloxacin traces; Mycoplasma gallisepticum; Tissue pharmacokinetic