title: Investigating tumor perfusion by hyperpolarized13C MRI with comparison to conventional gadolinium contrast-enhanced MRI and pathology in orthotopic human GBM xenografts.
abstract: Dissolution dynamic nuclear polarization (DNP) enables the acquisition of13C magnetic resonance data with a high sensitivity. Recently, metabolically inactive hyperpolarized13C-labeled compounds have shown to be potentially useful for perfusion imaging. The purpose of this study was to validate hyperpolarized perfusion imaging methods by comparing with conventional gadolinium (Gd)-based perfusion MRI techniques and pathology.
  
  
  
  Dynamic13C data using metabolically inactive hyperpolarized bis-1,1-(hydroxymethyl)-[1-13C]cyclopropane-d8(HMCP) were obtained from an orthotopic human glioblastoma (GBM) model for the characterization of tumor perfusion and compared with standard Gd-based dynamic susceptibility contrast (DSC) MRI data and immunohistochemical analysis from resected brains.
  
  
  
  Distinct HMCP perfusion characteristics were observed within the GBM tumors compared with contralateral normal brain tissue. The perfusion parameters obtained from the hyperpolarized HMCP data in tumor were strongly correlated with normalized peak height measured from the DSC images. The results from immunohistochemical analysis supported these findings by showing a high level of vascular staining for tumor that exhibited high levels of hyperpolarized HMCP signal.
  
  
  
  The results from this study have demonstrated that hyperpolarized HMCP data can be used as an indicator of tumor perfusion in an orthotopic xenograft model for GBM. Magn Reson Med 77:841-847, 2017. © 2016 International Society for Magnetic Resonance in Medicine.
authors.affiliation: Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA.
authors.forename: Ilwoo
authors.initials: I
authors.lastname: Park
authors.affiliation: Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA.
authors.forename: Cornelius
authors.initials: C
authors.lastname: von Morze
authors.affiliation: Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA.
authors.forename: Janine M
authors.initials: JM
authors.lastname: Lupo
authors.affiliation: GE Healthcare, Brøndby, Denmark.|Department of Electrical Engineering, Technical University of Denmark, Lyngby, Denmark.
authors.forename: Jan H
authors.initials: JH
authors.lastname: Ardenkjaer-Larsen
authors.affiliation: Media Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Achuta
authors.initials: A
authors.lastname: Kadambi
authors.affiliation: Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Vigneron
authors.affiliation: Surbeck Laboratory of Advanced Imaging, Department of Radiology and Biomedical Imaging, University of California, San Francisco, California, USA.|Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Nelson
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism*; D003287:Contrast Media / Q000378:metabolism*; D005682:Gadolinium / Q000378:metabolism; D005909:Glioblastoma / Q000000981:diagnostic imaging* / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051381:Rats; D011923:Rats, Nude
chemical_list: D002247:Carbon Isotopes; D003287:Contrast Media; D005682:Gadolinium
keywords: brain tumor; dynamic nuclear polarization; gadolinium dynamic susceptibility contrast imaging; hyperpolarized 13C perfusion MRI