title: The zebrafish fast myosin light chain mylpfa:H2B-GFP transgene is a useful tool for in vivo imaging of myocyte fusion in the vertebrate embryo.
abstract: Skeletal muscle fibers are multinucleated syncytia that arise from the fusion of mononucleated precursors, the myocytes, during embryonic development, muscle hypertrophy in post-embryonic growth and muscle regeneration after injury. Even though myocyte fusion is central to skeletal muscle differentiation, our current knowledge of the molecular mechanism of myocyte fusion in the vertebrates is rather limited. Previous work, from our group and others, has shown that the zebrafish embryo is a very useful model for investigating the cell biology and genetics of vertebrate myocyte fusion in vivo.
  
  
  
  Here, we report the generation of a stable transgenic zebrafish strain that expresses the Histone 2B-GFP (H2B-GFP) fusion protein in the nuclei of all fast-twitch muscle fibers under the control of the fast-twitch muscle-specific myosin light chain, phosphorylatable, fast skeletal muscle a (mylpfa) gene promoter. By introducing this transgene into a mutant for junctional adhesion molecule 3b (jam3b), which encodes a cell adhesion protein previously implicated in myocyte fusion, we demonstrate the feasibility of using this transgene for the analysis of myocyte fusion during the differentiation of the trunk musculature of the zebrafish embryo.
  
  
  
  Since we know so little about the molecules regulating vertebrate myocyte fusion, we propose that the mylpfa:H2B-GFP transgene will be a very useful reporter for conducting forward and reverse genetic screens to identify new components regulating vertebrate myocyte fusion.
authors.affiliation: Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, 138673, Singapore.
authors.forename: Weibin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, 138673, Singapore; Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, 119288, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore. Electronic address: sudipto@imcb.a-star.edu.sg.
authors.forename: Sudipto
authors.initials: S
authors.lastname: Roy
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics; D006657:Histones / Q000235:genetics; D024510:Muscle Development / Q000235:genetics*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018994:Myosin Light Chains / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D006657:Histones; C571344:Jam3b protein, zebrafish; D018994:Myosin Light Chains; D011956:Receptors, Cell Surface; D029961:Zebrafish Proteins; D049452:Green Fluorescent Proteins
keywords: Genetic screen; In vivo imaging; Zebrafish myocyte fusion; mylpfa:H2B-GFP transgene