title: Estrogen Deficiency Leads to Further Bone Loss in the Mandible of CKD Mice.
abstract: BACKGROUND
  Chronic kidney disease (CKD) has been regarded as a grave public health problem. Estrogen is a critical factor for both renal protection and bone remodeling. Our previous study demonstrated that CKD impairs the healing of titanium implants. The aim of this study was to investigate the effects of estrogen deficiency on the mandibular bone in CKD mice.
  
  
  METHODS
  Forty eleven-week-old female C57BL mice were used in this study. Uremia and estrogen deficiency were induced by 5/6 nephrectomy and ovariectomy (OVX), respectively. After 8 weeks, the mice were sacrificed, and their mandibles were collected for micro-CT analysis and histological examination.
  
  
  RESULTS
  All the mice survived the experimental period. Serum measurements confirmed a significant increase in BUN in the CKD group that was further increased by OVX. OVX led to significant decreases in both the BV/TV and cortical thickness of the mandibular bone in CKD mice.
  
  
  CONCLUSIONS
  In summary, our findings indicate that estrogen deficiency leads to further mandibular bone loss in CKD mice.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Ningyuan
authors.initials: N
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Duan
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
authors.forename: Dutmanee
authors.initials: D
authors.lastname: Seriwatanachai
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yongyue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000473:pathology*; D004967:Estrogens / Q000172:deficiency* / Q000378:metabolism; D005260:Female; D008334:Mandible / Q000000981:diagnostic imaging / Q000473:pathology*; D008810:Mice, Inbred C57BL; D010513:Periodontal Ligament / Q000000981:diagnostic imaging / Q000473:pathology; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000000981:diagnostic imaging; D013194:Staining and Labeling; D055114:X-Ray Microtomography
chemical_list: D004967:Estrogens