title: Simultaneous Dual Encoding of Three-Dimensional Structures by Light-Induced Modular Ligation.
abstract: A highly efficient strategy for the simultaneous dual surface encoding of 2D and 3D microscaffolds is reported. The combination of an oligo(ethylene glycol)-based network with two novel and readily synthesized monomers with photoreactive side chains yields two new photoresists, which can be used for the fabrication of microstructures (by two-photon polymerization) that exhibit a dual-photoreactive surface. By combining both functional photoresists into one scaffold, a dual functionalization pattern can be obtained by a single irradiation step in the presence of adequate reaction partners based on a self-sorting mechanism. The versatility of the approach is shown by the dual patterning of halogenated and fluorescent markers as well as proteins. Furthermore, we introduce a new ToF-SIMS mode ("delayed extraction") for the characterization of the obtained microstructures that combines high mass resolution with improved lateral resolution.
authors.affiliation: Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany.|Institut für Biologische Grenzflächen (IBG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Tanja K
authors.initials: TK
authors.lastname: Claus
authors.affiliation: Cell- and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Richter
authors.affiliation: Institute of Applied Physics and Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1, 76131, Karlsruhe, Germany.
authors.forename: Vincent
authors.initials: V
authors.lastname: Hahn
authors.affiliation: Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany. alexander.welle@kit.edu.|Institut für Biologische Grenzflächen (IBG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. alexander.welle@kit.edu.
authors.forename: Alexander
authors.initials: A
authors.lastname: Welle
authors.affiliation: ION-TOF GmbH, Heisenbergstrasse 15, 48149, Münster, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Kayser
authors.affiliation: Institute of Applied Physics and Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1, 76131, Karlsruhe, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Wegener
authors.affiliation: Cell- and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany.|Institut für Funktionelle Grenzflächen (IFG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Bastmeyer
authors.affiliation: Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany. guillaume.delaittre@kit.edu.|Institute for Toxicology and Genetics (ITG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. guillaume.delaittre@kit.edu.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Delaittre
authors.affiliation: Preparative Macromolecular Chemistry, Institut für Technische Chemie und Polymerchemie, Karlsruhe Institute of Technology (KIT), Engesserstrasse 18, 76131, Karlsruhe, Germany. christopher.barner-kowollik@kit.edu.|Institut für Biologische Grenzflächen (IBG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany. christopher.barner-kowollik@kit.edu.
authors.forename: Christopher
authors.initials: C
authors.lastname: Barner-Kowollik
keywords: cycloaddition; direct laser writing; microstructures; patterning; photochemistry