title: Echinococcus granulosus fatty acid binding proteins subcellular localization.
abstract: Two fatty acid binding proteins, EgFABP1 and EgFABP2, were isolated from the parasitic platyhelminth Echinococcus granulosus. These proteins bind fatty acids and have particular relevance in flatworms since de novo fatty acids synthesis is absent. Therefore platyhelminthes depend on the capture and intracellular distribution of host's lipids and fatty acid binding proteins could participate in lipid distribution. To elucidate EgFABP's roles, we investigated their intracellular distribution in the larval stage by a proteomic approach. Our results demonstrated the presence of EgFABP1 isoforms in cytosolic, nuclear, mitochondrial and microsomal fractions, suggesting that these molecules could be involved in several cellular processes.
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, C.P. 11400 Montevideo, Uruguay. Electronic address: gabial@fcien.edu.uy.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Alvite
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, C.P. 11400 Montevideo, Uruguay.
authors.forename: Adriana
authors.initials: A
authors.lastname: Esteves
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary; D048209:Echinococcus granulosus / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D050556:Fatty Acid-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006790:Host-Parasite Interactions; D007814:Larva / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D050356:Lipid Metabolism; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014583:Uruguay
chemical_list: D050556:Fatty Acid-Binding Proteins; D015801:Helminth Proteins; D020033:Protein Isoforms
keywords: Echinococcus granulosus; Fatty acid binding proteins