title: Cloning and characterization of a water deficit stress responsive transcription factor gene from Oryza sativa L.
abstract: Understanding the biochemical and molecular basis of drought mechanism in rice is important as drought is one of the major causes affecting rice crop adversely. A 1017 bp gene sequence encoding AP2/ERF family TF was isolated from Oryza sativa sp. Indica cv N22 encoding a protein of 338 amino acid residues, with a molecular weight of 36.58 kDa, and no intron in the ORF. The gene was named as AP2/ERF-N22(2) different from the drought responsive gene AP2/ERF-N22 that we reported earlier. AP2/ERF-N22(2) has entirely different characteristics from that of AP2/ERF-N22. It has a single AP2 domain of 55 amino acid residues and a cluster of acidic amino acid residues at the C-terminal region, which could function as a trans-activation domain. Presence of NLS indicates that it is a nuclear localized transcription factor encoding gene. It falls in group VI L, sharing characteristic similarities. Arabidopsis members of group VI L have been shown to be involved in response to cytokinin under drought stress.
authors.forename: Ibandalin
authors.initials: I
authors.lastname: Mawlong
authors.forename: Kishwar
authors.initials: K
authors.lastname: Ali
authors.forename: Aruna
authors.initials: A
authors.lastname: Tyagi
mesh_terms: D000595:Amino Acid Sequence; D003681:Dehydration; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins; D014157:Transcription Factors
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors