title: Fish condition factor, peroxisome proliferator activated receptors and biotransformation responses in Sarotherodon melanotheron from a contaminated freshwater dam (Awba Dam) in Ibadan, Nigeria.
abstract: The relationship between condition factor (CF), peroxisome proliferator-activated receptors (PPARs), phase 1 biotransformation (CYP1A isoforms) and contaminant burden has been studied in Sarotherodon melanotheron from a contaminated tropical freshwater dam (Awba Dam) and compared to a reference site (Modete Dam) in Southwest, Nigeria. A total of 89 fish (57 males and 32 females) was collected from Awba Dam and 95 fish (48 males and 47 females) from the reference site. In general, fish sampled from Awba Dam were bigger than reference site. Sediment samples were also collected from both sites for contaminant analysis. Expression of ppar and cyp1 isoforms was analyzed using validated real-time PCR, while CYP1A and PPAR protein levels were analyzed using immunochemical method with specific antibodies. CYP-mediated catalytic responses (EROD, MROD and BROD) were performed by biochemical methods. We observed significant increases in ppar and cyp1 isoforms mRNA in both male and female fish from Awba Dam, compared to the reference site. Catalytic activities of EROD, MROD and BROD paralleled cyp1 transcript levels. Sex-related differences in PPAR and CYP1A protein levels were also observed, showing higher CYP1A proteins in males, compared with females, and higher PPAR proteins in females compared with males. Principal component analysis (PCA) biplot showed positive relationships between biological responses (ppar isoforms), condition factor (CF) and sediment PCBs, PAHs, OCPs and heavy metal concentrations. The present study shows that S. melanotheron inhabiting Awba Dam are severely affected by different classes of environmental contaminants that target metabolic processes (PPAR) and biotransformation pathways (CYP1A) in male and female fish, compared to a reference site. Interestingly, fish from Awba Dam were exhibiting good growth (evidence by high CF values) that paralleled increases in the transcriptional activation of ppar and cyp1 isoforms, despite the high contaminant burdens, suggesting a possible contaminant-induced obesogenic effects.
authors.affiliation: Department of Zoology, University of Ibadan, Ibadan, Nigeria.
authors.forename: Aina O
authors.initials: AO
authors.lastname: Adeogun
authors.affiliation: Department of Zoology, University of Ibadan, Ibadan, Nigeria.
authors.forename: Oju R
authors.initials: OR
authors.lastname: Ibor
authors.affiliation: Department of Virology, University of Ibadan, Ibadan, Nigeria.
authors.forename: Anyebe B
authors.initials: AB
authors.lastname: Onoja
authors.affiliation: Department of Biology, Norwegian University of Science and Technology (NTNU), Høgskoleringen 5, N-7491 Trondheim, Norway. Electronic address: arukwe@bio.ntnu.no.
authors.forename: Augustine
authors.initials: A
authors.lastname: Arukwe
mesh_terms: D000818:Animals; D001711:Biotransformation / Q000502:physiology*; D023681:Cichlids / Q000502:physiology*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D005618:Fresh Water; D008297:Male; D019216:Metals, Heavy / Q000378:metabolism; D009549:Nigeria; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity
chemical_list: D019216:Metals, Heavy; D047492:Peroxisome Proliferator-Activated Receptors; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1
keywords: Aquatic environment; CYP1A; Condition factor; Developing country; Environmental obesogens; PPARs; Tilapia