title: Modified RNAs in CRISPR/Cas9: An Old Trick Works Again.
abstract: Old tricks, new dog: CRISPR/Cas9 is a powerful tool for gene editing that requires an endonuclease (Cas9) and RNA strands. It has been shown that chemical modification of the RNA structures, an approach that has been used to improve the efficiency of RNA interference, can also be applied to enhance the activity of CRISPR/Cas9 and reduce its off-target effects.
authors.affiliation: IMDEA Nanociencia & Nanobiotecnología (IMDEA-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC), Faraday 9, 28049, Madrid, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Latorre
authors.affiliation: IMDEA Nanociencia & Nanobiotecnología (IMDEA-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC), Faraday 9, 28049, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Latorre
authors.affiliation: IMDEA Nanociencia & Nanobiotecnología (IMDEA-Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CSIC), Faraday 9, 28049, Madrid, Spain. alvaro.somoza@imdea.org.
authors.forename: Álvaro
authors.identifier: http://orcid.org/0000-0001-9873-435X
authors.initials: Á
authors.lastname: Somoza
mesh_terms: D000818:Animals; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D000072669:Gene Editing; D006801:Humans; D012313:RNA / Q000235:genetics*
chemical_list: D012313:RNA
keywords: CRISPR/Cas; RNA; gene editing; nucleotide analogues; nucleotides