title: Real-Time Polymerase Chain Reaction in Stool Detects Transmission of Strongyloides stercoralis from an Infected Donor to Solid Organ Transplant Recipients.
abstract: Solid organ transplant recipients can acquire Strongyloides stercoralis from an infected donor. The diagnosis of S. stercoralis in immunocompromised individuals may be challenging due to a lower sensitivity of available parasitological and serological methods, compared with immunocompetent individuals. Recently, a real-time polymerase chain reaction (RT-PCR) in stool has been developed for S. stercoralis diagnosis. We report two cases of S. stercoralis infection transmitted by a donor to two solid organ transplant recipients, who were diagnosed with RT-PCR in stool. This test could play an important role inS. stercoralis diagnosis in immunosuppressed patients, facilitating rapid treatment initiation and reducing the risk of severe strongyloidiasis. Adherence to current recommendations of screening among donors and recipients from endemic areas is also urgently needed.
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain gjunyent@hotmail.com.
authors.forename: Joan
authors.initials: J
authors.lastname: Gómez-Junyent
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Paredes-Zapata
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Esperanza Rodríguez
authors.initials: ER
authors.lastname: de las Parras
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: José
authors.initials: J
authors.lastname: González-Costello
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Ruiz-Arranz
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Cañizares
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: Saugar
authors.affiliation: ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain; Donation and Transplant Coordination Section, Hospital Clinic-Universitat de Barcelona, Barcelona, Spain; Department of Parasitology, National Centre for Microbiology, Instituto de Salud Carlos III (CNM-ISCIII), Majadahonda, Madrid, Spain; Department of Cardiology, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain; Department of Intensive Care, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Hospital Universitari de Bellvitge, Universitat de Barcelona, Barcelona, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Muñoz
mesh_terms: D000368:Aged; D000818:Animals; D017809:Fatal Outcome; D005243:Feces / Q000469:parasitology*; D005260:Female; D016027:Heart Transplantation / Q000009:adverse effects*; D006801:Humans; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D017171:Strongyloides stercoralis; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000635:transmission; D014019:Tissue Donors