title: Vascular Endothelial Growth Factor Gene Polymorphism (rs2010963) and Its Receptor, Kinase Insert Domain-Containing Receptor Gene Polymorphism (rs2071559), and Markers of Carotid Atherosclerosis in Patients with Type 2 Diabetes Mellitus.
abstract: Background. The current study was designed to reveal possible associations between the polymorphisms of the vascular endothelial growth factor (VEGF) gene (rs2010963) and its receptor, kinase insert domain-containing receptor (KDR) gene polymorphism (rs2071559), and markers of carotid atherosclerosis in patients with type 2 diabetes mellitus (T2DM). Patients and Methods. 595 T2DM subjects and 200 control subjects were enrolled. The carotid intima-media thickness (CIMT) and plaque characteristics (presence and structure) were assessed ultrasonographically. Biochemical analyses were performed using standard biochemical methods. Genotyping of VEGF/KDR polymorphisms (rs2010963, rs2071559) was performed using KASPar assays. Results. Genotype distributions and allele frequencies of the VEGF/KDR polymorphisms (rs2010963, rs2071559) were not statistically significantly different between diabetic patients and controls. In our study, we demonstrated an association between the rs2071559 of KDR and either CIMT or the sum of plaque thickness in subjects with T2DM. We did not, however, demonstrate any association between the tested polymorphism of VEGF (rs2010963) and either CIMT, the sum of plaque thickness, the number of involved segments, hsCRP, the presence of carotid plaques, or the presence of unstable carotid plaques. Conclusions. In the present study, we demonstrated minor effect of the rs2071559 of KDR on markers of carotid atherosclerosis in subjects with T2DM.
authors.affiliation: Institute of Oncology Ljubljana, Zaloška 2, Sl-1000 Ljubljana, Slovenia.
authors.forename: Sebastjan
authors.initials: S
authors.lastname: Merlo
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine, University in Ljubljana, Vrazov trg 2, Sl-1000 Ljubljana, Slovenia.
authors.forename: Jovana Nikolajević
authors.initials: JN
authors.lastname: Starčević
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine, University in Ljubljana, Vrazov trg 2, Sl-1000 Ljubljana, Slovenia.
authors.forename: Sara
authors.initials: S
authors.lastname: Mankoč
authors.affiliation: General Hospital Rakičan, Ulica dr. Vrbnjaka 6, Sl-9000 Murska Sobota, Slovenia.
authors.forename: Marija
authors.initials: M
authors.lastname: Šantl Letonja
authors.affiliation: General Hospital Slovenj Gradec, Gosposvetska Cesta 1, Sl-2380 Slovenj Gradec, Slovenia.
authors.forename: Andreja
authors.initials: A
authors.lastname: Cokan Vujkovac
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine, University in Ljubljana, Vrazov trg 2, Sl-1000 Ljubljana, Slovenia.
authors.forename: Marjeta
authors.initials: M
authors.lastname: Zorc
authors.affiliation: Institute of Histology and Embryology, Faculty of Medicine, University in Ljubljana, Vrazov trg 2, Sl-1000 Ljubljana, Slovenia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Petrovič
mesh_terms: D000368:Aged; D002340:Carotid Artery Diseases / Q000235:genetics* / Q000473:pathology; D059168:Carotid Intima-Media Thickness; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000473:pathology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000235:genetics*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C000588866:KDR protein, human; D040301:Vascular Endothelial Growth Factor Receptor-2