title: [Diagnostic value of combined detection of HNF-1β and Napsin A in the diagnosis of ovarian clear cell carcinoma].
abstract: OBJECTIVE
  To study the diagnostic value of HNF-1β and Napsin A for ovarian clear cell carcinomas, serous carcinomas, endometrioid adenocarcinomas and metastatic Krukenberg tumors.
  
  
  METHODS
  Immunohistochemical EnVision method was used to detect the expression of HNF-1β and Napsin A in 38 cases of ovarian clear cell carcinoma, 30 cases of high-grade serous carcinoma, 22 cases of endometrioid adenocarcinoma and 16 cases of metastatic Krukenberg tumor. Expression of HNF-1β and Napsin A were compared, and sensitivity and specificity of clear cell carcinoma of the ovary were analysed.
  
  
  RESULTS
  The positive rate of HNF-1β in the ovarian clear cell carcinoma was 100%(38/38), higher than those in high-grade serous carcinoma and endometrioid adenocarcinoma (P<0.05), although significant difference was not observed from that of metastatic Krukenberg tumor (P>0.05). Napsin A expressed in 97.4% (37/38) of ovarian clear cell carcinoma, 6.7% (2/30) of high-grade serous carcinoma, 22.7% (5/22) of endometrioid adenocarcinoma. Napsin A expression in clear cell carcinoma was higher than those in high-grade serous carcinoma and endometrioid adenocarcinoma (P<0.01), and no expression of Napsin A was seen in metastatic Krukenberg tumor (P>0.05). The sensitivity and specificity of HNF-1β in the diagnosis of ovarian clear cell carcinoma were 100% and 52.9%, those of Napsin A were 97.4% and 91.2%, those of both HNF-1β and Napsin A were 97.4% and 91.2%, respectively. The sensitivity and specificity of HNF-1β or Napsin A in the diagnosis of ovarian clear cell carcinoma were 100% and 52.9%, respectively.
  
  
  CONCLUSIONS
  HNF-1β is a more sensitive marker for the diagnosis of ovarian clear cell carcinoma, whereas Napsin A is a more specific marker. The combined detection of HNF-1β and Napsin A may be helpful for the diagnosis of clear cell carcinoma of the ovary.
authors.affiliation: Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University, Nanjing 210006, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University, Nanjing 210006, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University, Nanjing 210006, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University, Nanjing 210006, China.
authors.forename: Kemei
authors.initials: K
authors.lastname: Xiong
authors.affiliation: Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University, Nanjing 210006, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Department of Pathology, Nanjing Hospital (Nanjing First Hospital), Nanjing Medical University, Nanjing 210006, China; E-mail: wbhuang348912@126.com.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Huang
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000175:diagnosis*; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D018269:Carcinoma, Endometrioid / Q000175:diagnosis; D018284:Cystadenocarcinoma, Serous / Q000175:diagnosis; D005260:Female; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007725:Krukenberg Tumor / Q000175:diagnosis; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D016282:Aspartic Acid Endopeptidases; C498549:NAPSA protein, human