title: Differential Effects of Colchicine on Cardiac Cell Viability in an in vitro Model Simulating Myocardial Infarction.
abstract: OBJECTIVE
  We aimed to examine the effects of colchicine, currently in clinical trials for acute myocardial infarction (AMI), on the viability of cardiac cells using a cell line model of AMI.
  
  
  METHODS
  HL-1, a murine cardiomyocyte cell line, and H9C2, a rat cardiomyoblast cell line, were incubated with TNFα or sera derived from rats that underwent AMI or sham operation followed by addition of colchicine. In another experiment, HL-1/H9C2 cells were exposed to anoxia with or without subsequent addition of colchicine. Cell morphology and viability were assessed by light microscopy, flow cytometry and Western blot analyses for apoptotic markers.
  
  
  RESULTS
  Cellular viability was similar in both sera; however, exposing both cell lines to anoxia reduced their viability. Adding colchicine to anoxic H9C2, but not to anoxic HL-1, further increased their mortality, at least in part via enhanced apoptosis. Under any condition, colchicine induced detachment of H9C2 cells from their culture plates. This phenomenon did not apply to HL-1 cells.
  
  
  CONCLUSIONS
  Colchicine enhanced cardiomyoblast mortality under in vitro conditions mimicking AMI and reduced their adherence capability. HL-1 was not affected by colchicine; nevertheless, no salvage effect was observed. We thus conclude that colchicine may not inhibit myocardial apoptosis following AMI.
authors.affiliation: Department of Internal Medicine H, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.
authors.forename: Gilad
authors.initials: G
authors.lastname: Margolis
authors.forename: Einat
authors.initials: E
authors.lastname: Hertzberg-Bigelman
authors.forename: Ran
authors.initials: R
authors.lastname: Levy
authors.forename: Jeremy
authors.initials: J
authors.lastname: Ben-Shoshan
authors.forename: Gad
authors.initials: G
authors.lastname: Keren
authors.forename: Michal
authors.initials: M
authors.lastname: Entin-Meer
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology*; D051379:Mice; D032386:Myoblasts, Cardiac / Q000187:drug effects / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D050257:Tubulin Modulators / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D050257:Tubulin Modulators; D014409:Tumor Necrosis Factor-alpha; D003078:Colchicine