title: Valorization of Palm Oil Industrial Waste as Feedstock for Lipase Production.
abstract: The use of residues from the industrial processing of palm oil as carbon source and inducer for microbial lipase production can be a way to add value to such residues and to contribute to reduced enzyme costs. The aim of this work was to investigate the feasibility of using palm oil industrial waste as feedstock for lipase production in different cultivation systems. Evaluation was made of lipase production by a selected strain of Aspergillus niger cultivated under solid-state (SSF) and submerged fermentation (SmF). Lipase activity levels up to 15.41 IU/mL were achieved under SSF. The effects of pH and temperature on the lipase activity of the SSF extract were evaluated using statistical design methodology, and maximum activities were obtained between pH 4.0 and 6.5 and at temperatures between 37 and 55 °C. This lipase presented good thermal stability up to 60 °C and higher specificity towards long carbon chain substrates. The results demonstrate the potential application of palm oil industrial residues for lipase production and contribute to the technological advances needed to develop processes for industrial enzymes production.
authors.affiliation: Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970, São Carlos, SP, Brazil.|Graduate Program of Biotechnology, Federal University of Sao Carlos, 13565-905, São Carlos, SP, Brazil.
authors.forename: Erick A
authors.initials: EA
authors.lastname: Silveira
authors.affiliation: Graduate Program of Chemical Engineering, Federal University of Sao Carlos, 13565-905, Sao Carlos, SP, Brazil.
authors.forename: Paulo W
authors.initials: PW
authors.lastname: Tardioli
authors.affiliation: Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970, São Carlos, SP, Brazil. cristiane.farinas@embrapa.br.|Graduate Program of Biotechnology, Federal University of Sao Carlos, 13565-905, São Carlos, SP, Brazil. cristiane.farinas@embrapa.br.|Graduate Program of Chemical Engineering, Federal University of Sao Carlos, 13565-905, Sao Carlos, SP, Brazil. cristiane.farinas@embrapa.br.
authors.forename: Cristiane S
authors.initials: CS
authors.lastname: Farinas
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D013696:Temperature
chemical_list: D007220:Industrial Waste; D010938:Plant Oils; D000073878:Palm Oil; D008049:Lipase
keywords: Agro-industrial wastes; Biorefinery; Lipase; Palm oil residues; Solid-state fermentation; Submerged fermentation