title: Effect of immobilization stress on the appetite and stomach ghrelin expression in maternal mice.
abstract: Maternal stress exerts long-lasting postnatal growth on offspring, which persist into adulthood. However, the effect of maternal stress on appetizing system has not been widely reported. In this study, we found that maternal immobilization stress (IS) during lactation resulted in low body weight and food intake. Immunohistochemistry showed an increase in stomach ghrelin protein expression. The central regulation of body weight and food intake occurs in the hypothalamus, which contains multiple neuronal systems that play important roles in the regulation of energy homeostasis. These systems including multiple neuropeptides involve in the ghrelin pathway of appetite regulation. Therefore, real time reverse transcription polymerase chain reaction (RT-PCR) was used to measure the change of mRNA expression of ghrelin pathway related hormones in order to explore the mechanisms involved in the appetite regulation. Expression levels of the hypothalamic 5-hydroxytryptamine 2c receptor (5-HT2cR) and 5-HT2bR, which are essential for the development and function of ghrelin and leptin, were decreased, as well as those of corticotrophin releasing factor (CRF) and pro-opiomelanocortin (POMC). While the expression of growth hormone secretagogue receptor (GHSR), neuropeptide-Y (NPY) and agouti-related protein (AgRP) showed an increase with significant difference. These results suggest that stress in a postpartum mother has persistent effects on the body weight of their offspring. Increased ghrelin and decreased leptin expression in the stomach may play a role in these effects.
authors.affiliation: Department of Central Laboratory, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of TCM, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Yuemei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of TCM, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Danqing
authors.initials: D
authors.lastname: Pan
authors.affiliation: Department of TCM, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Department of TCM, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of TCM, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Animal Experiments, Shanghai Public Health Clinical Center China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of TCM, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of TCM, Jinshan Hospital of Fudan University Shanghai, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Gao
mesh_terms: D054366:Agouti Signaling Protein / Q000378:metabolism; D000818:Animals; D001069:Appetite Regulation; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D004195:Disease Models, Animal; D004435:Eating; D005247:Feeding Behavior; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D054439:Ghrelin / Q000235:genetics / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism / Q000503:physiopathology; D007774:Lactation / Q000235:genetics / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism; D018811:Maternal Exposure; D008813:Mice, Inbred ICR; D009478:Neuropeptide Y / Q000378:metabolism; D049590:Postpartum Period; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D044403:Receptor, Serotonin, 5-HT2B / Q000378:metabolism; D044405:Receptor, Serotonin, 5-HT2C / Q000378:metabolism; D054440:Receptors, Ghrelin / Q000378:metabolism; D012149:Restraint, Physical / Q000523:psychology*; D013315:Stress, Psychological / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology / Q000523:psychology; D013997:Time Factors; D015430:Weight Gain
chemical_list: C000599853:5-hydroxytryptamine2C receptor, mouse; D054366:Agouti Signaling Protein; D054439:Ghrelin; D020738:Leptin; D009478:Neuropeptide Y; D044403:Receptor, Serotonin, 5-HT2B; D044405:Receptor, Serotonin, 5-HT2C; D054440:Receptors, Ghrelin; C515183:a protein, mouse; D011333:Pro-Opiomelanocortin; D003346:Corticotropin-Releasing Hormone
keywords: Maternal stress; appetizing system; ghrelin; stomach