title: Determination of valnemulin in swine and bovine tissues by ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: A sensitive and reliable method has been developed and validated for the determination of valnemulin in swine and bovine muscle, liver, and kidney using solid-phase extraction (SPE) and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The tissue samples were extracted with mixture solution of acetonitrile and 0.01mol/L hydrochloric acid, defatted by n-hexane, and further cleaned up using SPE cartridges with polymeric sorbent. Gradient UHPLC separation was performed using an Acquity BEH C18 column with water and acetonitrile as the mobile phase. Multiple reaction monitoring mode of two precursor-product ion transitions for valnemulin was used. Mean recoveries from fortified samples ranged from 93.4 to 104.3% with 3.3-10.7% relative standard deviation. The limit of detection and quantification was 0.2 and 1μg/kg for the analyte, respectively.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Shan
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Tianhe
authors.initials: T
authors.lastname: Liu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: xxia@cau.edu.cn.
authors.forename: Xi
authors.initials: X
authors.lastname: Xia
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis; D004350:Drug Residues / Q000032:analysis*; D059022:Food Safety; D057230:Limit of Detection; D000069466:Red Meat / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D004224:Diterpenes; C117845:valnemulin
keywords: Tandem mass spectrometry; Tissues; Ultra-high performance liquid chromatography; Valnemulin