title: Genetic mapping and molecular marker development for Pi65(t), a novel broad-spectrum resistance gene to rice blast using next-generation sequencing.
abstract: A novel R gene was mapped to a locus on chromosome 11 from 30.42 to 30.85 Mb, which was proven to be efficient in the improvement of rice blast resistance. Rice blast is a devastating fungal disease worldwide. The use of blast resistance (R) genes is the most important approach to control the disease in rice breeding. In the present study, we finely mapped a novel resistance gene Pi65(t), conferring a broad-spectrum resistance to the fungus Magnaporthe oryzae, using bulked segregant analysis in combination with next-generation sequencing technology. Segregation in a doubled haploid (DH) population and a BC1F2 population suggested that resistance to blast in Gangyu129 was likely conferred by a single dominant gene, designated Pi65(t); it was located on chromosome 11 from 30.20 to 31.20 Mb using next-generation sequencing. After screening recombinants with newly developed molecular markers, the region was narrowed down to 0.43 Mb, flanked by SNP-2 and SNP-8 at the physical location from 30.42 to 30.85 Mb based on the Nipponbare reference database in build 5. Using the software QTL IciMapping, Pi65(t) was further mapped to a locus between InDel-1 and SNP-4 with genetic distances of 0.11 and 0.98 cM, respectively. Within this region, 4 predicted R genes were found with nucleotide binding site and leucine-rich repeat (NBS-LRR) domains. We developed molecular markers to genotype 305 DH lines and found that InDel-1 was closely linked with Pi65(t). Using InDel-1, a new rice variety Chuangxin1 containing Pi65(t) was developed, and it is highly resistant to rice blast and produces a high yield in Liaoning province of China. This indicated that Pi65(t) could play a key role in the improvement of rice blast resistance.
authors.affiliation: Crops Molecular Improvement Laboratory, Liaoning Innovation Center of the Academy of Agricultural Sciences, 84 Dongling Road, Shenyang, 110161, China. zwj27@126.com.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Plant Protection College, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110161, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Shenyang Agricultural University, Shenyang, 110161, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Rice Research Institute, Shenyang Agricultural University, Shenyang, 110161, China.
authors.forename: Zuobin
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Crops Molecular Improvement Laboratory, Liaoning Innovation Center of the Academy of Agricultural Sciences, 84 Dongling Road, Shenyang, 110161, China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Crops Molecular Improvement Laboratory, Liaoning Innovation Center of the Academy of Agricultural Sciences, 84 Dongling Road, Shenyang, 110161, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Crops Molecular Improvement Laboratory, Liaoning Innovation Center of the Academy of Agricultural Sciences, 84 Dongling Road, Shenyang, 110161, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Plant Protection College, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110161, China.
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Crops Molecular Improvement Laboratory, Liaoning Innovation Center of the Academy of Agricultural Sciences, 84 Dongling Road, Shenyang, 110161, China. luxiaochun2000@126.com.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Lu
mesh_terms: D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing; D020082:Magnaporthe; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D010641:Phenotype; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers