title: Exosomes mediated pentose phosphate pathway in ovarian cancer metastasis: a proteomics analysis.
abstract: Epithelial ovarian cancer is the most lethal gynecological malignancies for readily metastasis. Exosomes have played an influential role in carcinogenicity and cancer progression. Our aim is to discover exosome-related mechanisms in ovarian cancer progress and explore potential diagnostic biomarkers and therapeutic targets of ovarian cancer. We initially presented the proteomic profiles of exosomes derived from two late-stage ovarian cell lines, OVCA429 and HO8910PM. A total of 2940 exosomal proteins were recorded by MS. FunRich appropriately processed these exosomal proteins, manifesting some superiority in contrast to Blast2go. Moreover, we demonstrated the pentose phosphate pathway was a dominant mechanism in exosome mediated intracellular communication. Glucose-6-phosphate dehydrogenase, transketolase and transaldolase 1, three key enzymes regulated pentose phosphate pathway, were all marked in the same exosomal parts of proteins between two ovarian cell lines. Moreover, these key proteins might become diagnostic, prognostic biomarkers and therapeutic targets of ovarian cancer.
authors.affiliation: Gynecological Oncology, Fujian Maternity and Children Health Hospital Fujian Medical University Teaching Hospital Fuzhou 350005, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yi
authors.affiliation: Gynecological Oncology, Fujian Maternity and Children Health Hospital Fujian Medical University Teaching Hospital Fuzhou 350005, China.
authors.forename: Xiangqin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Gynecological Oncology, Fujian Maternity and Children Health Hospital Fujian Medical University Teaching Hospital Fuzhou 350005, China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Song
authors.affiliation: The First Affiliated Hospital of Fujian Medical University Fuzhou 350005, China.
authors.forename: Rongkai
authors.initials: R
authors.lastname: Shen
authors.affiliation: Gynecological Oncology, Fujian Maternity and Children Health Hospital Fujian Medical University Teaching Hospital Fuzhou 350005, China.
authors.forename: Yanzhao
authors.initials: Y
authors.lastname: Su
authors.affiliation: Gynecological Oncology, Fujian Maternity and Children Health Hospital Fujian Medical University Teaching Hospital Fuzhou 350005, China.
authors.forename: Danmei
authors.initials: D
authors.lastname: Lin
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D004798:Enzymes / Q000378:metabolism*; D055354:Exosomes / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000201:enzymology* / Q000556:secondary; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000473:pathology; D010427:Pentose Phosphate Pathway; D040901:Proteomics / Q000379:methods*; D014153:Transaldolase / Q000378:metabolism; D014174:Transketolase / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D004798:Enzymes; D005954:Glucosephosphate Dehydrogenase; D014174:Transketolase; D014153:Transaldolase
keywords: Glucose-6-phosphate dehydrogenase (G6PD); Ovarian cancer; exosome; proteomics