title: Possibility of Using Mesenchymal Stromal Cells to Restore Lymph Flow in Experimental Phlebothrombosis.
abstract: The possibility of formation of lymphatic vessels after introduction of autologous bone marrow-derived multipotent mesenchymal stromal cells transfected with GFP gene into thrombosed femoral vein was studied by fluorescent microscopy. Vascular thrombosis caused by ligation of the great vein with subsequent injection of thrombin solution was accompanied by blockade of regional lymph flow. The cells injected into thrombosed vein directly participate in the formation of new lymphatic vessels in the paravasal tissue surrounding the vein, its tissue region, and around regional lymph nodes. This is seen from bright specific fluorescence of individual cells in the walls of lymphatic vessels and all vascular layers and valves in UV light.
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. imai@mail.ru.
authors.forename: I V
authors.initials: IV
authors.lastname: Maiborodin
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Morozov
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Matveeva
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Chastikin
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Moshak
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Onoprienko
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Seryapina
authors.affiliation: Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Anikeev
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D008198:Lymph Nodes / Q000166:cytology*; D042583:Lymphangiogenesis / Q000502:physiology*; D042601:Lymphatic Vessels / Q000473:pathology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D051381:Rats; D020246:Venous Thrombosis / Q000473:pathology / Q000628:therapy*
keywords: blockade the lymphatic vessels; lymphangiogenesis; mesenchymal stromal cells; venous thrombosis