title: Proteome Analysis of Disease Resistance against Ralstonia solanacearum in Potato Cultivar CT206-10.
abstract: Potato is one of the most important crops worldwide. Its commercial cultivars are highly susceptible to many fungal and bacterial diseases. Among these, bacterial wilt caused by Ralstonia solanacearum causes significant yield loss. In the present study, integrated proteomics and genomics approaches were used in order to identify bacterial wilt resistant genes from Rs resistance potato cultivar CT-206-10. 2-DE and MALDI-TOF/TOF-MS analysis identified eight differentially abundant proteins including glycine-rich RNA binding protein (GRP), tomato stress induced-1 (TSI-1) protein, pathogenesis-related (STH-2) protein and pentatricopeptide repeat containing (PPR) protein in response to Rs infection. Further, semi-quantitative RT-PCR identified up-regulation in transcript levels of all these genes upon Rs infection. Taken together, our results showed the involvement of the identified proteins in the Rs stress tolerance in potato. In the future, it would be interesting to raise the transgenic plants to further validate their involvement in resistance against Rs in potato.
authors.affiliation: Molecular Breeding Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 560-500, Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-707, Korea.
authors.forename: Ravi
authors.initials: R
authors.lastname: Gupta
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-707, Korea.
authors.forename: R
authors.initials: R
authors.lastname: Krishna
authors.affiliation: Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-707, Korea.
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Dong Yeol
authors.initials: DY
authors.lastname: Lee
authors.affiliation: Molecular Breeding Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 560-500, Korea.
authors.forename: Duk-Ju
authors.initials: DJ
authors.lastname: Hwang
authors.affiliation: Molecular Breeding Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 560-500, Korea.
authors.forename: Shin-Chul
authors.initials: SC
authors.lastname: Bae
authors.affiliation: Molecular Breeding Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 560-500, Korea.
authors.forename: Il-Pyung
authors.initials: IP
authors.lastname: Ahn
keywords: MALDI-TOF-MS; Ralstonia solanacearum; genomics; potato; proteomics