title: Structure and antinociceptive effects of β-D-glucans from Cookeina tricholoma.
abstract: Structurally different water-insoluble (1→3),(1→6) β-D-glucans were isolated from aqueous and alkaline extracts of the mushroom-forming ascomycete Cookeina tricholoma, a wild edible mushroom found in Brazilian Amazon forest. The structures showed different substitution patterns, which may influence their extractability and consequently their conformation in solution, and different MW (4.3×10(5)Da, 3.7×10(5)Da and 8.2×10(5)Da, for ICW-Ct, IHW-Ct and IK2-Ct, respectively). The main-chains are composed of (1→3)-linked β-D-Glcp units O-6 substituted by side chains with different lengths of (1→6)-linked β-d-Glcp units (ICW-Ct and IHW-Ct) or by a combination of (1→6)-linked β-D-Glcp units and single units of β-D-Glcp (IK2-Ct). β-D-glucans with similar MW and showing only (1→6)-linked β-D-Glcp units as side chains (ICW-Ct and IHW-Ct) showed significant inhibition of neurogenic pain, 69±11 and 57±11% at the dose of 10mgkg(-1), respectively, in the model of nociception induced by intraplantar injection of formalin.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba CEP 81531-980, PR, Brazil.
authors.forename: Roberta B
authors.initials: RB
authors.lastname: Moreno
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba CEP 81531-980, PR, Brazil; Division of Glycoscience, AlbaNova University Centre, Royal Institute of Technology, 106 91 Stockholm, Sweden.
authors.forename: Andrea C
authors.initials: AC
authors.lastname: Ruthes
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, CP 19031, CEP 81531-980 Curitiba, PR, Brazil.
authors.forename: Cristiane H
authors.initials: CH
authors.lastname: Baggio
authors.affiliation: Division of Glycoscience, AlbaNova University Centre, Royal Institute of Technology, 106 91 Stockholm, Sweden.
authors.forename: Francisco
authors.initials: F
authors.lastname: Vilaplana
authors.affiliation: Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo, 2936, CEP 69060-001 Manaus, AM, Brazil.
authors.forename: Dirce L
authors.initials: DL
authors.lastname: Komura
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba CEP 81531-980, PR, Brazil. Electronic address: iacomini@ufpr.br.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
mesh_terms: D000700:Analgesics / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D005260:Female; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D005936:Glucans / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D059225:Nociception / Q000187:drug effects
chemical_list: D000700:Analgesics; D062610:Fungal Polysaccharides; D005936:Glucans
keywords: (1→3),(1→6)-β-d-Glucans; Antinociceptive effect; Ascomycete; Cookeina tricholoma