title: Proliferation of Human Primary Myoblasts Is Associated with Altered Energy Metabolism in Dependence on Ageing In Vivo and In Vitro.
abstract: BACKGROUND. Ageing is associated with suppressed regenerative potential of muscle precursor cells due to decrease of satellite cells and suppressive intramuscular milieu on their activation, associated with ageing-related low-grade inflammation. The aim of the study was to characterize the function of oxidative phosphorylation (OXPHOS), glycolysis, adenylate kinase (AK), and creatine kinase (CK) mediated systems in young and older individuals. MATERIALS AND METHODS. Myoblasts were cultivated from biopsies taken by transcutaneous conchotomy from vastus lateralis muscle in young (20-29 yrs, n = 7) and older (70-79 yrs, n = 7) subjects. Energy metabolism was assessed in passages 2 to 6 by oxygraphy and enzyme analysis. RESULTS. In myoblasts of young and older subjects the rate of OXPHOS decreased during proliferation from passages 2 to 6. The total activities of CK and AK decreased. Myoblasts of passage 2 cultivated from young muscle showed higher rate of OXPHOS and activities of CK and AK compared to myoblasts from older subjects while hexokinase and pyruvate kinase were not affected by ageing. CONCLUSIONS. Proliferation of myoblasts in vitro is associated with downregulation of OXPHOS and energy storage and transfer systems. Ageing in vivo exerts an impact on satellite cells which results in altered metabolic profile in favour of the prevalence of glycolytic pathways over mitochondrial OXPHOS of myoblasts.
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia; Department of Traumatology and Orthopaedics, Tartu University Hospital, L. Puusepa 8, 51014 Tartu, Estonia; Department of Traumatology and Orthopaedics, University of Tartu, L. Puusepa 8, 51014 Tartu, Estonia.
authors.forename: Reedik
authors.identifier: 0000-0002-0360-7727
authors.initials: R
authors.lastname: Pääsuke
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Margus
authors.identifier: 0000-0002-0656-9295
authors.initials: M
authors.lastname: Eimre
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Andres
authors.initials: A
authors.lastname: Piirsoo
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Nadežda
authors.initials: N
authors.lastname: Peet
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Liidia
authors.initials: L
authors.lastname: Laada
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Lumme
authors.initials: L
authors.lastname: Kadaja
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Mart
authors.initials: M
authors.lastname: Roosimaa
authors.affiliation: Institute of Exercise Biology and Physiotherapy, University of Tartu, Ravila 14a, 50411 Tartu, Estonia.
authors.forename: Mati
authors.identifier: 0000-0001-9465-2605
authors.initials: M
authors.lastname: Pääsuke
authors.affiliation: Department of Traumatology and Orthopaedics, Tartu University Hospital, L. Puusepa 8, 51014 Tartu, Estonia; Department of Traumatology and Orthopaedics, University of Tartu, L. Puusepa 8, 51014 Tartu, Estonia.
authors.forename: Aare
authors.initials: A
authors.lastname: Märtson
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Enn
authors.initials: E
authors.lastname: Seppet
authors.affiliation: Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia.
authors.forename: Kalju
authors.initials: K
authors.lastname: Paju
mesh_terms: D000263:Adenylate Kinase / Q000378:metabolism; D000328:Adult; D000367:Age Factors; D000368:Aged; D000375:Aging; D000818:Animals; D001706:Biopsy; D049109:Cell Proliferation; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D004734:Energy Metabolism; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D007249:Inflammation; D018482:Muscle, Skeletal / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010100:Oxygen / Q000737:chemistry; D011770:Pyruvate Kinase / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology; D055815:Young Adult
chemical_list: D006593:Hexokinase; D011770:Pyruvate Kinase; D003402:Creatine Kinase; D000263:Adenylate Kinase; D010100:Oxygen