title: Analysis of the Rab GTPase Interactome in Dendritic Cells Reveals Anti-microbial Functions of the Rab32 Complex in Bacterial Containment.
abstract: Dendritic cells (DCs) orchestrate complex membrane trafficking through an interconnected transportation network linked together by Rab GTPases. Through a tandem affinity purification strategy and mass spectrometry, we depicted an interactomic landscape of major members of the mammalian Rab GTPase family. When complemented with imaging tools, this proteomic analysis provided a global view of intracellular membrane organization. Driven by this analysis, we investigated dynamic changes to the Rab32 subnetwork in DCs induced by L. monocytogenes infection and uncovered an essential role of this subnetwork in controlling the intracellular proliferation of L. monocytogenes. Mechanistically, Rab32 formed a persistent complex with two interacting proteins, PHB and PHB2, to encompass bacteria both during early phagosome formation and after L. monocytogenes escaped the original containment vacuole. Collectively, we have provided a functional compartmentalization overview and an organizational framework of intracellular Rab-mediated vesicle trafficking that can serve as a resource for future investigations.
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Zou
authors.affiliation: Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin 300000 China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Tang
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Qingzhu
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Ni
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Lizhang
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Regina
authors.initials: R
authors.lastname: Lin
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Wong
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Liting
authors.initials: L
authors.lastname: Wang
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Quanfang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Ran
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Hengning
authors.initials: H
authors.lastname: Lian
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Immunology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Chongqing Key Laboratory of Cytomics, Chongqing 400038, China; Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA. Electronic address: qi-jing.li@duke.edu.
authors.forename: Qi-Jing
authors.initials: QJ
authors.lastname: Li
authors.affiliation: Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Cytomics, Chongqing 400038, China. Electronic address: wanying.cn@gmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wan
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use; D002460:Cell Line; D019295:Computational Biology; D003264:Containment of Biohazards; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D008089:Listeria monocytogenes / Q000254:growth & development / Q000276:immunology*; D008088:Listeriosis / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D046912:Multiprotein Complexes / Q000378:metabolism*; D000091402:Prohibitins; D021381:Protein Transport; D012097:Repressor Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D046912:Multiprotein Complexes; C000717333:Phb2 protein, mouse; D000091402:Prohibitins; D012097:Repressor Proteins; D000217:Acyltransferases; C060970:poly-beta-hydroxybutyrate polymerase; C478766:Rab32 protein, mouse; D020691:rab GTP-Binding Proteins