title: Baicalin ameliorates isoproterenol-induced acute myocardial infarction through iNOS, inflammation, oxidative stress and P38MAPK pathway in rat.
abstract: Baicalin is one of the active ingredients in the skullcap, with a variety of pharmacological effects, such as blood pressure reduction, sedation, liver-protection, gallbladder-protection, anti-bacteria, anti-inflammation, etc. The aim of this study was to investigate the potential cardioprotective effects of baicalin ameliorates isoproterenol-induced acute myocardial infarction (AMI) through inducible nitric oxide synthase (iNOS), inflammation, oxidative stress and P38MAPK passageway in rat. Rat model of AMI was induced by isoproterenol (100 mg/kg) and then treated baicalin (various does of baicalin: 1 mg/kg, 10 mg/kg and 100 mg/kg, respectively) for 24 h. Infarct size, the heart weight to body weight ratio and creatine kinase (CK), the MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) of rats with AMI induced by isoproterenol were used to evaluate curative effect of baicalin on AMI. Meanwhile, iNOS and phosphorylation-p38 MAPK (p-p38) protein expressions, inflammatory factor and oxidative stress were inspected using western blot and commercial kits, respectively. In the present study, pre-treatment with baicalin (10 or 100 mg/kg) significantly ameliorated infarct size, the heart weight to body weight ratio and CK, CK-MB, LDH and cTnT levels in rats with AMI induced by isoproterenol. iNOS protein expression, the serum TNF-α, IL-6, MDA and SOD levels and p-38 protein expressions were significantly suppressed by treatment with baicalin (10 or 100 mg/kg). These results suggest that acute treatment with baicalin ameliorates AMI, iNOS, inflammation, oxidative stress and P38MAPK pathway in rat with AMI induced by isoproterenol.
authors.affiliation: Department of Cardiology, The Second Hospital Affiliated to Zhengzhou University Zhengzhou 450014, Henan, China.
authors.forename: Shen-Jie
authors.initials: SJ
authors.lastname: Sun
authors.affiliation: Department of Cardiology, The Second Hospital Affiliated to Zhengzhou University Zhengzhou 450014, Henan, China.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Wu
authors.affiliation: Department of Cardiology, The Second Hospital Affiliated to Zhengzhou University Zhengzhou 450014, Henan, China.
authors.forename: Heng-Liang
authors.initials: HL
authors.lastname: Song
authors.affiliation: Department of Cardiology, The Second Hospital Affiliated to Zhengzhou University Zhengzhou 450014, Henan, China.
authors.forename: Gui-Qi
authors.initials: GQ
authors.lastname: Li
keywords: Baicalin; acute myocardial infarction; iNOS; inflammation; isoproterenol; oxidative stress