title: Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research.
abstract: Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. This article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, and identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA.
authors.forename: Degao
authors.initials: D
authors.lastname: Liu
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA.
authors.forename: Rongbin
authors.initials: R
authors.lastname: Hu
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA.
authors.forename: Kaitlin J
authors.initials: KJ
authors.lastname: Palla
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA.
authors.forename: Gerald A
authors.initials: GA
authors.lastname: Tuskan
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA. Electronic address: yangx@ornl.gov.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Yang
mesh_terms: D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D018745:Genome, Plant / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D008954:Models, Biological; D030821:Plants, Genetically Modified / Q000235:genetics*