title: Purification, Characterization, and Optimum Conditions of Fermencin SD11, a Bacteriocin Produced by Human Orally Lactobacillus fermentum SD11.
abstract: Fermencin SD11, a bacteriocin produced by human orally Lactobacillus fermentum SD11, was purified, characterized, and optimized in conditions for bacterial growth and bacteriocin production. Fermencin SD11 was purified using three steps of ammonium sulfate precipitation, gel filtration chromatography, and reverse-phase high-performance liquid chromatography. The molecular weight was found to be 33,000 Da using SDS-PAGE and confirmed as 33,593.4 Da by liquid chromatography-mass spectrometry. Fermencin SD11 exhibited activity against a wide range of oral pathogens including cariogenic and periodontogenic pathogens and Candida. The active activity was stable between 60 - 80 °C in a pH range of 3.0 to 7.0. It was sensitive to proteolytic enzymes (proteinase K and trypsin), but it was not affected by α-amylase, catalase, lysozyme, and saliva. The optimum conditions for growth and bacteriocin production of L. fermentum SD11 were cultured at acidic with pH of 5.0-6.0 at 37 or 40 °C under aerobic or anaerobic conditions for 12 h. It is promising that L. fermentum SD11 and its bacteriocin may be an alternative approach for promoting oral health or prevention of oral diseases, e.g., dental caries and periodontitis, which would require further clinical trials.
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, 90112, Thailand.
authors.forename: Phirawat
authors.initials: P
authors.lastname: Wannun
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, 90112, Thailand.
authors.forename: Supatcharin
authors.initials: S
authors.lastname: Piwat
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, 90112, Thailand. rawee.t@psu.ac.th.
authors.forename: Rawee
authors.initials: R
authors.lastname: Teanpaisan
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003731:Dental Caries / Q000382:microbiology* / Q000628:therapy; D005285:Fermentation; D006801:Humans; D052197:Limosilactobacillus fermentum / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity; D010518:Periodontitis / Q000382:microbiology* / Q000628:therapy
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C000613737:fermencin SD11 protein, Lactobacillus fermentum; D000645:Ammonium Sulfate
keywords: Bacteriocin; Characterization; Fermencin SD11; Lactobacillus fermentum; Optimum conditions; Oral diseases; Purification