title: Long non-coding RNA MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation in colorectal cancer cells.
abstract: Our earlier findings indicate that the long non-coding RNA MALAT1 promotes colorectal cancer (CRC) cell proliferation, invasion and metastasis in vitro and in vivo by increasing expression of AKAP-9. In the present study, we investigated the molecular mechanism by which MALAT1 enhances AKAP9 expression in CRC SW480 cells. We found that MALAT1 interacts with both SRPK1 and SRSF1. MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation. Following MALAT1 knockdown, overexpression of SRPK1 was sufficient to restore SRSF1 phosphorylation and AKAP-9 expression to a level that promoted cell proliferation, invasion and migration in vitro. Conversely, SRPK1 knockdown after overexpression of MALAT1 in SW480 cells diminished SRSF1 phosphorylation and AKAP-9 expression and suppressed cell proliferation, invasion and migration in vitro. These findings suggest MALAT1 increases AKAP-9 expression by promoting SRPK1-catalyzed SRSF1 phosphorylation in CRC cells. These results reveal a novel molecular mechanism by which MALAT1 regulates AKAP-9 expression in CRC cells.
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Hu
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.|Department of Urology, The Third Affiliated Hospital of Southern Medical University, Southern Medical University, Guangzhou, China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Guo
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Lin-Ying
authors.initials: LY
authors.lastname: Xie
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Huang
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Liu
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Xiao
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Li
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.|Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.|Guangdong Provincial Key Laboratory of Molecular Tumour Pathology, Southern Medical University, Guangzhou, China.
authors.forename: Zu-Guo
authors.initials: ZG
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Heping
authors.initials: H
authors.lastname: Kan
mesh_terms: D054758:A Kinase Anchor Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D000068103:Serine-Arginine Splicing Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D054758:A Kinase Anchor Proteins; C119719:AKAP9 protein, human; D003598:Cytoskeletal Proteins; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; C000595779:SRSF1 protein, human; D000068103:Serine-Arginine Splicing Factors; C087728:SRPK1 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: AKAP-9; MALAT-1; SRSF1; colorectal cancer; long non-coding RNA