title: VKORC1 and CD-14 genetic polymorphisms associate with susceptibility to cardiovascular and cerebrovascular diseases.
abstract: OBJECTIVE
  To investigate the associations of VKORC1 rs2359612 and rs9923231 and CD-14 rs2569190 with susceptibility to cardiovascular and cerebrovascular diseases (CCVD).
  
  
  METHODS
  A case-control study was conducted with 614 cases of CCVD patients selected at our hospital between January 2011 and June 2012 as case group and 590 healthy individuals participating physical examination during the same period as control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to detect genotypes of VKORC1 and CD-14 genetic polymorphisms. SHEsis software was used to conduct haplotype analysis and logistic regression analysis was used to identify risk factors for CCVD.
  
  
  RESULTS
  The genotype and allele frequencies of VKORC1 rs2359612 and rs9923231 and CD-14 rs2569190 between the case and control groups were statistically different (all P<0.05). Haplotype analysis showed that the frequencies of CAT and TAT haplotypes were significantly higher while the frequencies of TAC and TGC haplotypes were significantly lower in the case group than those in the control group (P = 0.013, 0.029, 0.019 and 0.042, respectively). Logistic regression analysis showed that age, systolic pressure, smoking history and VKORC1 rs2359612 maybe risk factors for CCVD; and body mass index (BMI), diastolic pressure and VKORC1 rs9923231 may be protective factors for CCVD (all P<0.05).
  
  
  CONCLUSIONS
  VKORC1 rs2359612 and rs9923231, and CD-14 rs2569190 might associate with susceptibility to CCVD. CAT and TAT haplotypes may be risk factors while TAC and TGC haplotype may be protective factors for CCVD.
authors.affiliation: Department of Geriatrics, The First Hospital of Jilin University Changchun 130021, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Geriatrics, The First Hospital of Jilin University Changchun 130021, P. R. China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Geriatrics, The First Hospital of Jilin University Changchun 130021, P. R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Geriatrics, The First Hospital of Jilin University Changchun 130021, P. R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhen
authors.affiliation: Department of Geriatrics, The First Hospital of Jilin University Changchun 130021, P. R. China.
authors.forename: Jiyan
authors.initials: J
authors.lastname: Leng
authors.affiliation: Department of Geriatrics, The First Hospital of Jilin University Changchun 130021, P. R. China.
authors.forename: Jianmeng
authors.initials: J
authors.lastname: Wang
keywords: CD-14 gene polymorphism; Logistic regression analysis; VKORC1 gene polymorphism; cardiovascular and cerebrovascular diseases; haplotype analysis; rs2359612; rs2569190; rs9923231