title: Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide from Lepidium meyenii.
abstract: A novel polysaccharide named as MC-1 was isolated from the roots of Lepidium meyenii using a water extraction method. Structural characterization revealed that MC-1 had an average molecular weight of 11.3 kDa and consisted of arabinose (26.21%), mannose (11.81%), glucose (53.66%), and galactose (8.32%). The main linkage types of MC-1 were proven to be (1 → 5)-α-L-Ara, (1 → 3)-α-L-Man, (1 → 2,6)-α-L-Man, (1 → )-α-D-Glc, (1 → 4)-α-D-Glc, (1 → 6)-α-D-Glc and (1 → 6)-β-D-Gal by methylation analysis, periodate oxidation-Smith degradation and NMR analysis. The immunostimulating assay indicated that MC-1 could significantly enhance the pinocytic and phagocytic capacity and promote the NO, TNF-α, and IL-6 secretion of RAW 264.7 cells, involving toll-like receptor 2, complement receptor 3, and mannose receptor mainly. These results suggested the potential utilization of MC-1 as an attractive functional food supplement candidate for hypoimmunity population.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou, Guangdong 510640, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou, Guangdong 510640, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou, Guangdong 510640, China.|Guangdong Provincial Key Laboratory of Green Agricultural Products Processing, Guangzhou, Guangdong 510640, China.
authors.forename: Furao
authors.initials: F
authors.lastname: Lai
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou, Guangdong 510640, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D001089:Arabinose / Q000032:analysis; D002236:Carbohydrate Conformation; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D007155:Immunologic Factors / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D029686:Lepidium / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008358:Mannose / Q000032:analysis; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010873:Pinocytosis / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D007155:Immunologic Factors; D015850:Interleukin-6; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D005690:Galactose
keywords: Lepidium meyenii; immunomodulatory activity; polysaccharide; structural characterization