title: Highly efficient and selective enrichment of glycopeptides using easily synthesized magG/PDA/Au/l-Cys composites.
abstract: Highly selective and efficient enrichment of glycopeptides from complex biological samples is necessary. In this study, novel zwitterionic hydrophilic polydopamine-coated magnetic graphene composites (magG/PDA/Au/l-Cys) were synthesized and applied to the enrichment of glycopeptides. The size, morphology, and composition of magG/PDA/Au/l-Cys composites were investigated by transmission electron microscopy, scanning electron microscopy, FT-infrared spectroscopy, and X-ray diffraction. The composites possessed a number of desirable characteristics, including good biocompatibility easy separation property and excellent hydrophilicity. By virtue of the features contributed by different ingredients, the prepared composites demonstrated superior performance for glycopeptide enrichment with high sensitivity (0.1 fmol), efficiency, selectivity (1:100), and repeatability (at least ten times). In addition, the composites were successfully applied to the enrichment of glycopeptides from human serum and 40 unique N-glycosylation peptides from 31 different N-linked glycoproteins were identified. The superior hydrophilic material is of great potential for the analysis of glycoproteins.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai, China.
authors.forename: Runqing
authors.initials: R
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Fudan University, Shanghai, China.
authors.forename: Lanting
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, P. R. China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000302:isolation & purification*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D003545:Cysteine / Q000737:chemistry; D006020:Glycopeptides / Q000302:isolation & purification*; D006031:Glycosylation; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry*; D059346:Magnets; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins; D006020:Glycopeptides; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D006046:Gold; D006108:Graphite; D003545:Cysteine
keywords: Enrichment; Glycopeptide; Glycoproteomics; Graphene; Hydrophilicity; Polydopamine