title: Simultaneous determination of urinary parabens, bisphenol A, triclosan, and 8-hydroxy-2'-deoxyguanosine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
abstract: A simple and fast method was developed for the simultaneous determination of five parabens, bisphenol A (BPA), triclosan (TCS), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in human urine using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The solid-phase extraction (SPE) procedure, chromatographic conditions, and MS/MS parameters were optimized to achieve maximum sensitivity and accuracy for the analytes. The validation results showed that the correlation coefficients (R (2)) and recoveries ranged from 0.999 to 1 and 83.9 to 109.9 %, respectively, and the intra-day and inter-day precisions (relative standard deviation, RSD) were within the range of 1.3-8.5 % and 1.3-9.0 %, respectively. The limits of detection for the analytes ranged from 0.001 to 0.05 μg/L. The method was successfully employed to determine parabens, BPA, TCS, and 8-OHdG in urine samples from school students in Guangzhou, China. The results showed that methyl, ethyl, n-propyl parabens, BPA, TCS, and 8-OHdG were frequently detected in urine samples. n-Butyl and benzyl parabens were only detected in a part of the samples due to their low concentrations in urine.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou, Guangdong, 510006, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Ren
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou, Guangdong, 510006, China.
authors.forename: Jianzhang
authors.initials: J
authors.lastname: Fang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China.
authors.forename: Guihua
authors.initials: G
authors.lastname: Liu
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China.
authors.forename: Honghe
authors.initials: H
authors.lastname: Liu
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lin
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong, 518055, China. lushaoyou2005@163.com.
authors.forename: Shaoyou
authors.initials: S
authors.lastname: Lu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D001559:Benzhydryl Compounds / Q000652:urine*; D002853:Chromatography, Liquid / Q000379:methods*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D010226:Parabens / Q000032:analysis*; D010636:Phenols / Q000652:urine*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D001559:Benzhydryl Compounds; D003849:Deoxyguanosine; D010226:Parabens; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: 8-Hydroxy-2′-deoxyguanosine; Bisphenol A; Parabens; Triclosan; Urine