title: Genomic characterization and assessment of the virulence and antibiotic resistance of the novel species Paenibacillus sp. strain VT-400, a potentially pathogenic bacterium in the oral cavity of patients with hematological malignancies.
abstract: BACKGROUND
  Paenibacillus sp. strain VT-400, a novel spore-forming bacterium, was isolated from patients with hematological malignancies.
  
  
  METHODS
  Paenibacillus sp. strain VT-400 was isolated from the saliva of four children with acute lymphoblastic leukemia. The genome was annotated using RAST and the NCBI Prokaryotic Genome Annotation Pipeline to characterize features of antibiotic resistance and virulence factors. Susceptibility to antibiotics was determined by the Kirby-Bauer disc diffusion method. We used a mouse model of pneumonia to study virulence in vivo. Mice were challenged with 7.5 log10-9.5 log10 CFU, and survival was monitored over 7 days. Bacterial load was measured in the lungs and spleen of surviving mice 48 h post-infection to reveal bacterial invasion and dissemination.
  
  
  RESULTS
  Whole-genome sequencing revealed a large number of virulence factors such as hemolysin D and CD4+ T cell-stimulating antigen. Furthermore, the strain harbors numerous antibiotic resistance genes, including small multidrug resistance proteins, which have never been previously found in the Paenibacillus genus. We then compared the presence of antibiotic resistance genes against results from antibiotic susceptibility testing. Paenibacillus sp. strain VT-400 was found to be resistant to macrolides such as erythromycin and azithromycin, as well as to chloramphenicol and trimethoprim-sulphamethoxazole. Finally, the isolate caused mortality in mice infected with ≥8.5 log10 CFU.
  
  
  CONCLUSIONS
  Based on our results and on the available literature, there is yet no strong evidence that shows Paenibacillus species as an opportunistic pathogen in immunocompromised patients. However, the presence of spore-forming bacteria with virulence and antibiotic resistance genes in such patients warrants special attention because infections caused by spore-forming bacteria are poorly treatable.
authors.affiliation: Institute of Human Microbiology, LLC, 303 5th Avenue, Suite 2012, New York, NY 10016 USA.
authors.forename: George
authors.identifier: 0000-0003-3205-9018
authors.initials: G
authors.lastname: Tetz
authors.affiliation: First State I. P. Pavlov Medical University, Lev Tolstoy Str. 6/8, Saint Petersburg, Russia 197022.
authors.forename: Victor
authors.initials: V
authors.lastname: Tetz
authors.affiliation: First State I. P. Pavlov Medical University, Lev Tolstoy Str. 6/8, Saint Petersburg, Russia 197022.
authors.forename: Maria
authors.initials: M
authors.lastname: Vecherkovskaya
keywords: Antibiotic resistance; Hematological malignancies; Immunocompromised; Nosocomial; Paenibacillus sp.; Pathogen; Pneumonia