title: Raman spectroscopy identifies radiation response in human non-small cell lung cancer xenografts.
abstract: External beam radiation therapy is a standard form of treatment for numerous cancers. Despite this, there are no approved methods to account for patient specific radiation sensitivity. In this report, Raman spectroscopy (RS) was used to identify radiation-induced biochemical changes in human non-small cell lung cancer xenografts. Chemometric analysis revealed unique radiation-related Raman signatures that were specific to nucleic acid, lipid, protein and carbohydrate spectral features. Among these changes was a dramatic shift in the accumulation of glycogen spectral bands for doses of 5 or 15 Gy when compared to unirradiated tumours. When spatial mapping was applied in this analysis there was considerable variability as we found substantial intra- and inter-tumour heterogeneity in the distribution of glycogen and other RS spectral features. Collectively, these data provide unique insight into the biochemical response of tumours, irradiated in vivo, and demonstrate the utility of RS for detecting distinct radiobiological responses in human tumour xenografts.
authors.affiliation: University of Victoria, Department of Physics and Astronomy, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada.
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Harder
authors.affiliation: University of Victoria, Department of Physics and Astronomy, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Isabelle
authors.affiliation: BC Cancer Agency-Vancouver Island Centre, Trev and Joyce Deeley Research Centre, 2410 Lee Ave., Victoria, British Columbia, V8R 6V5, Canada.
authors.forename: Lindsay
authors.initials: L
authors.lastname: DeVorkin
authors.affiliation: BC Cancer Agency-Vancouver Island Centre, Trev and Joyce Deeley Research Centre, 2410 Lee Ave., Victoria, British Columbia, V8R 6V5, Canada.
authors.forename: Julian
authors.initials: J
authors.lastname: Smazynski
authors.affiliation: University of Victoria, Department of Physics and Astronomy, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada.|BC Cancer Agency-Vancouver Island Centre, Medical Physics, 2410 Lee Ave., Victoria, British Columbia, V8R 6V5, Canada.
authors.forename: Wayne
authors.initials: W
authors.lastname: Beckham
authors.affiliation: University of Victoria, Department of Chemistry, PO Box 3065, Victoria, British Columbia, V8W 3V6, Canada.
authors.forename: Alexandre G
authors.initials: AG
authors.lastname: Brolo
authors.affiliation: BC Cancer Agency-Vancouver Island Centre, Trev and Joyce Deeley Research Centre, 2410 Lee Ave., Victoria, British Columbia, V8R 6V5, Canada.|University of Victoria, Department of Biochemistry and Microbiology, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada.
authors.forename: Julian J
authors.initials: JJ
authors.lastname: Lum
authors.affiliation: Mathematics, Statistics, Physics, and Computer Science, University of British Columbia Okanagan, 3333 University Way, Kelowna, British Columbia, V1V 1V7, Canada.
authors.forename: Andrew
authors.initials: A
authors.lastname: Jirasek
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000528:radiation effects; D005260:Female; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D055432:Metabolomics / Q000379:methods; D009928:Organ Specificity; D025341:Principal Component Analysis; D011836:Radiation Tolerance; D013059:Spectrum Analysis, Raman / Q000379:methods; D023041:Xenograft Model Antitumor Assays
chemical_list: D006003:Glycogen