title: Nuclear anomalies in exfoliated buccal epithelial cells of petrol station attendants in Udaipur, Rajasthan.
abstract: OBJECTIVE
  The petroleum derivatives consist of a complex mixture of chemical compounds one among which is benzene. Petrol station workers who pump fuel to vehicles absorb the products of fuel fumes and the products of combustion.
  
  
  METHODS
  To study the occupational exposure to petroleum derivatives such as benzene, exfoliated buccal cells from 40 petrol station attendants and 40 age-matched control subjects were examined for micronuclei, binucleation, karyorrhexis and karyolysis frequency by using feulgen and Giemsa stains. Statistical evaluation was performed with ANOVA test.
  
  
  RESULTS
  In the present study, inter-comparison of mean values for micronuclei, binucleation, karyorrhexis and karyolysis using Fuelgen stain between smokers of study and control group, as well as between smokers and non-smokers of study and control group revealed statistically highly significant results with P value 0.00002 and 0.0001 respectively. Whereas inter comparison between non-smokers of study and control group using Feulgen stain and inter comparison between smokers of study and control group, using Giemsa stain revealed statistically significant results with P value 0.0034 and 0.0004, respectively.
  
  
  CONCLUSIONS
  This study revealed that the studied individuals belong to a risk group and should periodically undergo biological monitoring and proper care.
authors.affiliation: Department of Oral Pathology and Microbiology, Pacific Dental College and Hospital, Udaipur, Rajasthan, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Metgud
authors.forename: Nidhi
authors.initials: N
authors.lastname: Khajuria
authors.forename: Shrikant
authors.initials: S
authors.lastname: Patel
authors.forename: Sahul
authors.initials: S
authors.lastname: Lerra
mesh_terms: D016022:Case-Control Studies; D002467:Cell Nucleus / Q000187:drug effects* / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000187:drug effects*; D016273:Occupational Exposure / Q000009:adverse effects*; D010578:Petroleum / Q000009:adverse effects*
chemical_list: D010578:Petroleum