title: The effects of plasma electrolytically oxidized NiTi on in vitro endothelialization.
abstract: The role of biomaterials surface in controlling the interfacial biological events leading to implant integration is of key importance. In this study, the effects of NiTi surfaces treated by plasma electrolytic oxidation (PEO) on human umbilical vein endothelial cells (HUVECs) have been investigated. The changes in NiTi surface morphology and chemistry were assessed by SEM, XPS and cross-section TEM/EDX analyzes whereas the effects of the resultant surfaces on in vitro endothelialization and cell junction proteins have been evaluated by life/dead staining, SEM, cells counting, qPCR and immunofluorescence. The findings indicated that the PEO-treated NiTi, with a microporous morphology and oxide dominated surface chemistry, supports viability and proliferation of HUVECs. Numerous thin filopodia probing the microporous surface assisted cells attachment. In addition, claudin-5 and occludin have been upregulated and expression of vascular endothelial-cadherin was not suppressed on PEO-treated NiTi relative to the reference electropolished surfaces. The results of this study suggest that novel NiTi surfaces may be developed using the PEO process, which can be of benefit to atherosclerosis treatment.
authors.affiliation: Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Huan
authors.affiliation: Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.affiliation: Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China. Electronic address: haiyan.li@sjtu.edu.cn.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: M S
authors.initials: MS
authors.lastname: Ruiter
authors.affiliation: Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China; Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
authors.forename: J
authors.initials: J
authors.lastname: Chang
authors.affiliation: Department of BioMechanical Engineering, Delft University of Technology, Mekelweg 2, 2628 CD Delft, The Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: Apachitei
authors.affiliation: Department of BioMechanical Engineering, Delft University of Technology, Mekelweg 2, 2628 CD Delft, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Duszczyk
authors.affiliation: Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands.
authors.forename: C J M
authors.initials: CJM
authors.lastname: de Vries
authors.affiliation: Department of BioMechanical Engineering, Delft University of Technology, Mekelweg 2, 2628 CD Delft, The Netherlands. Electronic address: e.l.fratila-apachitei@tudelft.nl.
authors.forename: L E
authors.initials: LE
authors.lastname: Fratila-Apachitei
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000494:pharmacology; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D062507:Claudin-5 / Q000235:genetics / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D004572:Electrolysis; D004573:Electrolytes / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009532:Nickel / Q000737:chemistry / Q000494:pharmacology*; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D056951:Photoelectron Spectroscopy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*; D000099152:Cadherin 5
chemical_list: D000497:Alloys; D015703:Antigens, CD; D015820:Cadherins; D062507:Claudin-5; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D009532:Nickel; D062793:Occludin; D014025:Titanium; D000099152:Cadherin 5; C040654:titanium nickelide
keywords: Endothelial cells; Junction proteins; Nitinol; Plasma electrolytic oxidation; Stents