title: Production of new 3D scaffolds for bone tissue regeneration by rapid prototyping.
abstract: The incidence of bone disorders, whether due to trauma or pathology, has been trending upward with the aging of the worldwide population. The currently available treatments for bone injuries are rather limited, involving mainly bone grafts and implants. A particularly promising approach for bone regeneration uses rapid prototyping (RP) technologies to produce 3D scaffolds with highly controlled structure and orientation, based on computer-aided design models or medical data. Herein, tricalcium phosphate (TCP)/alginate scaffolds were produced using RP and subsequently their physicochemical, mechanical and biological properties were characterized. The results showed that 60/40 of TCP and alginate formulation was able to match the compression and present a similar Young modulus to that of trabecular bone while presenting an adequate biocompatibility. Moreover, the biomineralization ability, roughness and macro and microporosity of scaffolds allowed cell anchoring and proliferation at their surface, as well as cell migration to its interior, processes that are fundamental for osteointegration and bone regeneration.
authors.affiliation: CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Fradique
authors.affiliation: CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
authors.forename: T R
authors.initials: TR
authors.lastname: Correia
authors.affiliation: CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
authors.forename: S P
authors.initials: SP
authors.lastname: Miguel
authors.affiliation: CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
authors.forename: K D
authors.initials: KD
authors.lastname: de Sá
authors.affiliation: CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
authors.forename: D R
authors.initials: DR
authors.lastname: Figueira
authors.affiliation: CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.|Department of Chemistry, University of Beira Interior, R. Marquês d'Ávila e Bolama, 6201-001, Covilhã, Portugal.
authors.forename: A G
authors.initials: AG
authors.lastname: Mendonça
authors.affiliation: CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal. icorreia@fcsaude.ubi.pt.
authors.forename: I J
authors.initials: IJ
authors.lastname: Correia
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology; D001861:Bone Regeneration / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D010758:Phosphorus / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D010758:Phosphorus; D002118:Calcium