title: Hypoxic conditioned medium of placenta-derived mesenchymal stem cells protects against scar formation.
abstract: OBJECTIVE
  Scar formation after wound repair affects people's daily life. Mesenchymal stem cells were reported to have a beneficial role in attenuating the scar formation. In the present study, placenta-derived mesenchymal stem cells (PMSCs) were isolated and the effects of hypoxic conditioned medium of PMSCs on scar formation were explored.
  
  
  METHODS
  To evaluate the effect of hypoxia on PMSCs, proliferation of PMSCs was detected by trypan blue staining and the HIF-1α level was detected by western blot. Then in vivo scar formation assay was performed and the histopathologic changes were evaluated by HE staining and levels of TGF-β1 and collagen I were detected by quantitative real-time PCR. The IL-10 level was detected by ELISA and then migration of HFF-1 cells was detected by wound healing assay after treatment with IL-10 or IL-10 antibody.
  
  
  RESULTS
  Our study showed that hypoxic conditioned medium of PMSCs reduced scar formation in vivo and inhibited the proliferation and migration of skin fibroblasts in vitro. Further mechanism study showed that, the level of IL-10 was affected by hypoxia, treatment with IL-10 mimicked the function of hypoxic conditioned medium of PMSCs and inhibition of IL-10 reversed the protective role of hypoxic conditioned medium of PMSCs. Thus, hypoxic conditioned medium of PMSCs may perform the protective role against scar formation through IL-10.
  
  
  CONCLUSIONS
  Our study reveals a possible mechanism of the protective effect of PMSCs against scar formation and provides evidence for the hypothesis that PMSCs may be a promising therapy for the treatment of wounds.
authors.affiliation: Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang 110122, People's Republic of China. Electronic address: dllbinglishengli@126.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Du
authors.affiliation: Department of Spinal Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Runxiao
authors.initials: R
authors.lastname: Lv
authors.affiliation: Seven-year System, Department of Clinical Medicine, China Medical University, Shenyang 110122, People's Republic of China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Shaohang
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang 110122, People's Republic of China.
authors.forename: Tingxian
authors.initials: T
authors.lastname: Ma
authors.affiliation: Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D015687:Cell Hypoxia / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002921:Cicatrix / Q000378:metabolism* / Q000517:prevention & control*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010920:Placenta / Q000166:cytology* / Q000502:physiology*; D011247:Pregnancy
chemical_list: D017077:Culture Media, Conditioned
keywords: Hypoxia; IL-10; Migration; Placenta-derived mesenchymal stem cells; Proliferation; Scar formation; Skin fibroblasts