title: Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses.
abstract: Endogenous retroviruses are repetitive sequences found abundantly in mammalian genomes which are capable of modulating host gene expression. Nevertheless, most endogenous retrovirus copies are under tight epigenetic control via histone-repressive modifications and DNA methylation. Here we describe a common method used in our laboratory to detect, quantify, and compare mammalian endogenous retrovirus DNA methylation. More specifically we describe methylated DNA immunoprecipitation (MeDIP) followed by quantitative PCR.
authors.affiliation: Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, Canada, V5Z 1L3. rrebollo@bccrc.ca.|Department of Medical Genetics, University of British Columbia, Vancouver, Canada, V6H 3N1. rrebollo@bccrc.ca.
authors.forename: Rita
authors.initials: R
authors.lastname: Rebollo
authors.affiliation: Terry Fox Laboratory, British Columbia Cancer Agency, 675 West 10th Avenue, Vancouver, BC, Canada, V5Z 1L3. dmager@bccrc.ca.|Department of Medical Genetics, University of British Columbia, Vancouver, Canada, V6H 3N1. dmager@bccrc.ca.
authors.forename: Dixie L
authors.initials: DL
authors.lastname: Mager
mesh_terms: D000818:Animals; D019175:DNA Methylation; D020077:Endogenous Retroviruses / Q000235:genetics* / Q000378:metabolism*; D044127:Epigenesis, Genetic; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D008322:Mammals; D060888:Real-Time Polymerase Chain Reaction
keywords: DNA methylation; Endogenous retroviruses; In vitro methylation; MeDIP