title: Catalytic chemiluminescent detection of cholesterol in serum with Cu2-xSe semiconductor nanoparticles.
abstract: It is very important to be able to accurately and rapidly measure the cholesterol level in the human body, as cholesterol is associated with various diseases, such as Alzheimer's disease. In this work, a novel method of detecting cholesterol using chemiluminescence (CL) based on a newly prepared semiconductor catalyst, Cu2-xSe nanoparticles (Cu2-xSe NPs), was developed. It was found that the Cu2-xSe NPs strongly enhanced the CL signal by producing a large number of reactive oxygen species (ROS) in the luminol-Cu2-xSe NPs system. Based on the UV-vis-NIR absorption spectra, zeta potential, CL spectrum, and an investigation of the ROS, a possible mechanism for the CL was proposed. This CL-based method was successfully applied to determine cholesterol. It was found that the enhanced CL was proportional to the concentration of cholesterol over the range of 82 nM to 1.96 μM with a detection limit of 0.062 nM, and that added cholesterol was successfully detected in human serum with a mean recovery of 97 %.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
authors.forename: Fang Fang
authors.initials: FF
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China.
authors.forename: Hong Yan
authors.initials: HY
authors.lastname: Zou
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lan
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China. chengzhi@swu.edu.cn.|College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China. chengzhi@swu.edu.cn.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
mesh_terms: D000085:Acetates / Q000737:chemistry; D001681:Biological Assay; D002384:Catalysis; D002784:Cholesterol / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000737:chemistry; D018036:Selenium Compounds / Q000737:chemistry*
chemical_list: D000085:Acetates; D017382:Reactive Oxygen Species; D018036:Selenium Compounds; D008165:Luminol; C477347:cupric selenide; C007650:ethyl acetate; D002784:Cholesterol
keywords: Catalyst; Chemiluminescence; Cholesterol; Cu2−x Se NPs