title: The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana.
abstract: Nucleolar proteins play important roles in plant cytology, growth, and development. Fibrillarin2 is a nucleolar protein of Nicotiana benthamiana (N. benthamiana). Its cDNA was amplified by RT-PCR and inserted into expression vector pEarley101 labeled with yellow fluorescent protein (YFP). The fusion protein was localized in the nucleolus and Cajal body of leaf epidermal cells of N. benthamiana. The N. benthamiana fibrillarin2 (NbFib2) protein has three functional domains (i.e., glycine and arginine rich domain, RNA-binding domain, and α-helical domain) and a nuclear localization signal (NLS) in C-terminal. The protein 3D structure analysis predicted that NbFib2 is an α/β protein. In addition, the virus induced gene silencing (VIGS) approach was used to determine the function of NbFib2. Our results showed that symptoms including growth retardation, organ deformation, chlorosis, and necrosis appeared in NbFib2-silenced N. benthamiana.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Institute of Plant Protection, Fujian Provincial Academy of Agricultural Sciences, Fuzhou 350002, China.
authors.forename: Jinai
authors.initials: J
authors.lastname: Yao
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Fangluan
authors.initials: F
authors.lastname: Gao
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Lian
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Xie
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Plant Virology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
mesh_terms: D000595:Amino Acid Sequence; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D008780:Methyltransferases