title: Albumin and apolipoprotein H mRNAs in human plasma as potential clinical biomarkers of liver injury: analyses of plasma liver-specific mRNAs in patients with liver injury.
abstract: BACKGROUND
  Plasma liver-specific mRNAs are useful biomarkers of hepatotoxicity in rats.
  
  
  OBJECTIVE
  To investigate the potential application of liver-specific mRNAs as biomarkers for liver injury in humans.
  
  
  METHODS
  We determined the plasma levels of liver-specific mRNAs by real-time qRT-PCR in healthy donors and patients with liver injury.
  
  
  RESULTS
  Plasma levels of albumin (ALB) and apolipoprotein H (APOH) mRNAs increased in patients with elevated serum alanine aminotransferase. These mRNAs also increased in plasma after transcatheter arterial chemoembolization, which induces specific injury to liver.
  
  
  CONCLUSIONS
  We demonstrated the potential application of plasma ALB and APOH mRNAs as clinical biomarkers for liver injury.
authors.affiliation: a Drug Safety Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited , Fujisawa , Japan and.
authors.forename: Shingo
authors.initials: S
authors.lastname: Okubo
authors.affiliation: a Drug Safety Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited , Fujisawa , Japan and.
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyamoto
authors.affiliation: b Department of Gastroenterology and Hepatology , Osakafu Saiseikai Nakatsu Hospital , Osaka , Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Ito
authors.affiliation: a Drug Safety Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited , Fujisawa , Japan and.
authors.forename: Kenji
authors.initials: K
authors.lastname: Takami
authors.affiliation: b Department of Gastroenterology and Hepatology , Osakafu Saiseikai Nakatsu Hospital , Osaka , Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ashida
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism*; D005260:Female; D006801:Humans; D008099:Liver / Q000293:injuries* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012709:Serum Albumin / Q000378:metabolism*; D014947:Wounds and Injuries / Q000097:blood
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D012709:Serum Albumin
keywords: Circulating mRNAs; TACE; clinical application; liver injury; real-time qRT-PCR