title: D-Lactic acid production by Sporolactobacillus inulinus YBS1-5 with simultaneous utilization of cottonseed meal and corncob residue.
abstract: d-Lactic acid, is an important organic acid produced from agro-industrial wastes by Sporolactobacillus inulinus YBS1-5 was investigated to reduce the raw material cost of fermentation. The YBS1-5 strain could produce d-lactic acid by using cottonseed meal as the sole nitrogen source. For efficient utilization, the cottonseed meal was enzymatically hydrolyzed and simultaneously utilized during d-lactic acid fermentation. Corncob residues are rich in cellulose and can be enzymatically hydrolyzed without pretreatment. The hydrolysate of this lignocellulosic waste could be utilized by strain YBS1-5 as a carbon source for d-lactic acid production. Under optimal conditions, a high d-lactic acid concentration (107.2g/L) was obtained in 7-L fed-batch fermenter, with an average productivity of 1.19g/L/h and a yield of 0.85g/g glucose. The optical purity of d-lactic acid in the broth was 99.2%. This study presented a new approach for low-cost production of d-lactic acid for an industrial application.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
authors.forename: Zhongzhong
authors.initials: Z
authors.lastname: Bai
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China. Electronic address: wubin1977@njtech.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
authors.forename: Bingfang
authors.initials: B
authors.lastname: He
mesh_terms: D056449:Bacillales / Q000378:metabolism*; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D003368:Gossypium / Q000378:metabolism*; D006868:Hydrolysis; D007220:Industrial Waste; D019344:Lactic Acid / Q000096:biosynthesis*; D009584:Nitrogen / Q000737:chemistry; D018505:Waste Management / Q000379:methods; D003313:Zea mays / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D019344:Lactic Acid; D002244:Carbon; D002482:Cellulose; D005947:Glucose; D009584:Nitrogen
keywords: Corncob residue; Cottonseed meal; Sporolactobacillus inulinus; d-Lactic acid