title: In vitro selection of DNA aptamers binding pesticide fluoroacetamide.
abstract: Fluoroacetamide (Mw = 77.06) is a lethal rodenticide to humans and animals which is still frequently abused in food storage somewhere in China. The production of antibodies for fluoroacetamide is difficult due to its high toxicity to animals, which limits the application of immunoassay method in poison detection. In this work, aptamers targeting N-fluoroacetyl glycine as an analog of fluoroacetamide were selected by a specific systematic evolution of ligands by exponential enrichment (SELEX) strategy. The binding ability of the selected aptamers to fluoroacetamide was identified using surface plasmon resonance (SPR)-based assay. The estimated KD values in the low micromolar range showed a good affinity of these aptamers to the target. Our work verified that the SELEX strategy has the potential for developing aptamers targeted to small molecular toxicants and aptamers can be employed as new recognition elements instead of antibodies for poison detection.
authors.affiliation: a Shanghai Key Laboratory of Crime Scene Evidence , Shanghai Research Institute of Criminal Science and Technology , Shanghai , China.
authors.forename: Fangqi
authors.initials: F
authors.lastname: Cao
authors.affiliation: b Shanghai Key Laboratory of Crime Scene Evidence , Shanghai Institute of Forensic Science , Shanghai , China.|c State Key Laboratory of New Drug and Pharmaceutical Process , Shanghai Institute of Pharmaceutical Industry , Shanghai , China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Lu
authors.affiliation: b Shanghai Key Laboratory of Crime Scene Evidence , Shanghai Institute of Forensic Science , Shanghai , China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Hu
authors.affiliation: b Shanghai Key Laboratory of Crime Scene Evidence , Shanghai Institute of Forensic Science , Shanghai , China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: b Shanghai Key Laboratory of Crime Scene Evidence , Shanghai Institute of Forensic Science , Shanghai , China.
authors.forename: Libo
authors.initials: L
authors.lastname: Zeng
authors.affiliation: b Shanghai Key Laboratory of Crime Scene Evidence , Shanghai Institute of Forensic Science , Shanghai , China.
authors.forename: Liankang
authors.initials: L
authors.lastname: Chen
authors.affiliation: c State Key Laboratory of New Drug and Pharmaceutical Process , Shanghai Institute of Pharmaceutical Industry , Shanghai , China.
authors.forename: Meiqi
authors.initials: M
authors.lastname: Sun
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry*; D001483:Base Sequence; D001681:Biological Assay; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry*; D005463:Fluoroacetates / Q000302:isolation & purification*; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008024:Ligands; D018716:Molecular Mimicry; D009690:Nucleic Acid Conformation; D012378:Rodenticides / Q000302:isolation & purification*; D052156:SELEX Aptamer Technique; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012710:Serum Albumin, Bovine / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D005463:Fluoroacetates; D008024:Ligands; D012378:Rodenticides; D012710:Serum Albumin, Bovine; C025271:monofluoroacetylglycine; C007741:fluoroacetamide; D005998:Glycine
keywords: SELEX; SPR; aptamer; fluoroacetamide; small molecular toxicant