title: Hexavalent chromium removal and bioelectricity generation by Ochrobactrum sp. YC211 under different oxygen conditions.
abstract: Bioremediation is an environmentally friendly method of reducing heavy metal concentration and toxicity. A chromium-reducing bacterial strain, isolated from the vicinity of an electroplate factory, was identified as Ochrobactrum sp. YC211. The efficiency and capacity per time of Ochrobactrum sp. YC211 for hexavalent chromium (Cr(VI)) removal under anaerobic conditions were superior to those under aerobic conditions. An acceptable removal efficiency (96.5 ± 0.6%) corresponding to 30.2 ± 0.8 mg-Cr (g-dry cell weight-h)(-1) was achieved by Ochrobactrum sp. YC211 at 300 mg L(-1) Cr(VI). A temperature of 30°C and pH 7 were the optimal parameters for Cr(VI) removal. By examining reactivated cells, permeabilized cells, and cell-free extract, we determined that Cr(VI) removal by Ochrobactrum sp. YC211 under anaerobic conditions mainly occurred in the soluble fraction of the cell and can be regarded as an enzymatic reaction. The results also indicated that an Ochrobactrum sp. YC211 microbial fuel cell (MFC) with an anaerobic anode was considerably superior to that with an aerobic anode in bioelectricity generation and Cr(VI) removal. The maximum power density and Cr(VI) removal efficiency of the MFC were 445 ± 3.2 mW m(-2) and 97.2 ± 0.3%, respectively. Additionally, the effects of coexisting ions (Cu(2+), Zn(2+), Ni(2+), SO4(2-), and Cl(-)) in the anolyte on the MFC performance and Cr(VI) removal were nonsignificant (P > 0.05). To our knowledge, this is the first report to compare Cr(VI) removal by different cells and MFC types under aerobic and anaerobic conditions.
authors.affiliation: a Department of Tourism and Leisure , Hsing Wu University , Taipei , Taiwan.
authors.forename: Chih-Yu
authors.initials: CY
authors.lastname: Chen
authors.affiliation: b Department of Biological Science and Technology , China University of Science and Technology , Taipei , Taiwan.
authors.forename: Chiu-Yu
authors.initials: CY
authors.lastname: Cheng
authors.affiliation: c Department of Chemical Engineering and Biotechnology , National Taipei University of Technology , Taipei , Taiwan.
authors.forename: Ching-Kuo
authors.initials: CK
authors.lastname: Chen
authors.affiliation: b Department of Biological Science and Technology , China University of Science and Technology , Taipei , Taiwan.
authors.forename: Min-Chi
authors.initials: MC
authors.lastname: Hsieh
authors.affiliation: b Department of Biological Science and Technology , China University of Science and Technology , Taipei , Taiwan.
authors.forename: Ssu-Ting
authors.initials: ST
authors.lastname: Lin
authors.affiliation: b Department of Biological Science and Technology , China University of Science and Technology , Taipei , Taiwan.
authors.forename: Kuo-Ying
authors.initials: KY
authors.lastname: Ho
authors.affiliation: b Department of Biological Science and Technology , China University of Science and Technology , Taipei , Taiwan.
authors.forename: Jo-Wei
authors.initials: JW
authors.lastname: Li
authors.affiliation: b Department of Biological Science and Technology , China University of Science and Technology , Taipei , Taiwan.
authors.forename: Chia-Pei
authors.initials: CP
authors.lastname: Lin
authors.affiliation: b Department of Biological Science and Technology , China University of Science and Technology , Taipei , Taiwan.
authors.forename: Ying-Chien
authors.initials: YC
authors.lastname: Chung
mesh_terms: D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002274:Carcinogens, Environmental / Q000378:metabolism*; D002857:Chromium / Q000378:metabolism*; D004595:Electroplating; D007220:Industrial Waste / Q000032:analysis; D019216:Metals, Heavy / Q000378:metabolism*; D042041:Ochrobactrum / Q000378:metabolism*; D012722:Sewage / Q000032:analysis / Q000737:chemistry; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism; D013624:Taiwan
chemical_list: D002274:Carcinogens, Environmental; D007220:Industrial Waste; D019216:Metals, Heavy; D012722:Sewage; D012989:Soil Pollutants; D002857:Chromium
keywords: Bioremediation; chromium; microbial fuel cell; permeabilized cell