title: Detection of circulating tumor cells using GeneScan analysis for antigen receptor gene rearrangements in canine lymphoma patients.
abstract: The presence of circulating tumor cells (CTCs) serves as a prognostic marker and indicator of disease relapse, as well as a means of evaluating treatment efficacy in human and canine lymphoma patients. As an extension of our previous study for the construction of clinically useful GeneScan system, we utilized the GeneScan system for detecting CTCs in canine lymphoma patients. Samples from the primary lesion and peripheral blood mononuclear cells (PBMCs) were obtained from 32 dogs with lymphoma at initial diagnosis. All samples were subjected to polymerase chain reaction (PCR) for antigen receptor gene rearrangements (PARR) followed by GeneScan analysis. Common clonal rearrangements with identical amplified fragments were detected in both the primary lesion and PBMCs in 19 of the 32 dogs (59.4%). However, the detection rate of CTCs varied among the anatomical classification of lymphoma studied. GeneScan analysis following PARR would facilitate studies on determining the clinical significance of CTCs in canine lymphoma patients.
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Saaya
authors.initials: S
authors.lastname: Hiyoshi-Kanemoto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Goto-Koshino
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Fukushima
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kanemoto
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Uchida
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Fujino
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohno
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000235:genetics; D004285:Dogs; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008223:Lymphoma / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D008297:Male; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D007143:Immunoglobulin Heavy Chains