title: High mycobacterial diversity in recreational lakes.
abstract: Although nontuberculous mycobacteria (NTM) are natural inhabitants of freshwater ecosystems, few studies have focused on their distribution in these habitats. Thus, the knowledge about the abundance as well as the composition of NTM remains limited and patchy in these environments. In this context, a prospective study was performed to identify favourable habitats for mycobacteria in two recreational lakes. Mycobacterial density and diversity were measured using quantitative real-time PCR and the MiSeq Illumina platform. For both lakes, five compartments were investigated, i.e. water column, air-water interface, sediment, epilithon and epiphyton biofilms. Nontuberculous mycobacteria were detected in all compartments in large densities and displayed a remarkable diversity. NTM were dominated by fast-growing species. Lakes and compartments appeared to shape mycobacteria assemblage composition as well as their densities. In both lakes, some OTUs assigned to the species level were identified as related to known opportunistic pathogens.
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (LEESU UMR MA 102), Faculté des Sciences et Technologie, Université Paris-Est, AgroParisTech, 61 avenue du Général de Gaulle, 94000, Créteil, France. adelaide.roguet@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Roguet
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (LEESU UMR MA 102), Faculté des Sciences et Technologie, Université Paris-Est, AgroParisTech, 61 avenue du Général de Gaulle, 94000, Créteil, France.
authors.forename: C
authors.initials: C
authors.lastname: Therial
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (LEESU UMR MA 102), Faculté des Sciences et Technologie, Université Paris-Est, AgroParisTech, 61 avenue du Général de Gaulle, 94000, Créteil, France.
authors.forename: M
authors.initials: M
authors.lastname: Saad
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (LEESU UMR MA 102), Faculté des Sciences et Technologie, Université Paris-Est, AgroParisTech, 61 avenue du Général de Gaulle, 94000, Créteil, France.
authors.forename: L
authors.initials: L
authors.lastname: Boudahmane
authors.affiliation: Eau de Paris, Direction Recherche et Développement Qualité de l'Eau (DRDQE), 33 avenue Jean Jaurès, 94200, Ivry-Sur-Seine, France.
authors.forename: L
authors.initials: L
authors.lastname: Moulin
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (LEESU UMR MA 102), Faculté des Sciences et Technologie, Université Paris-Est, AgroParisTech, 61 avenue du Général de Gaulle, 94000, Créteil, France.
authors.forename: F S
authors.initials: FS
authors.lastname: Lucas
mesh_terms: D044822:Biodiversity; D018441:Biofilms; D017753:Ecosystem; D005602:France; D019015:Geologic Sediments / Q000382:microbiology; D060106:Lakes / Q000382:microbiology*; D009161:Mycobacterium / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D009170:Nontuberculous Mycobacteria / Q000737:chemistry / Q000235:genetics / Q000254:growth & development; D011446:Prospective Studies; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: High-throughput sequencing; Lake; Nontuberculous mycobacteria; Quantitative real-time PCR