title: TBP/TFIID-dependent activation of MyoD target genes in skeletal muscle cells.
abstract: Change in the identity of the components of the transcription pre-initiation complex is proposed to control cell type-specific gene expression. Replacement of the canonical TFIID-TBP complex with TRF3/TBP2 was reported to be required for activation of muscle-gene expression. The lack of a developmental phenotype in TBP2 null mice prompted further analysis to determine whether TBP2 deficiency can compromise adult myogenesis. We show here that TBP2 null mice have an intact regeneration potential upon injury and that TBP2 is not expressed in established C2C12 muscle cell or in primary mouse MuSCs. While TFIID subunits and TBP are downregulated during myoblast differentiation, reduced amounts of these proteins form a complex that is detectable on promoters of muscle genes and is essential for their expression. This evidence demonstrates that TBP2 does not replace TBP during muscle differentiation, as previously proposed, with limiting amounts of TFIID-TBP being required to promote muscle-specific gene expression.
authors.affiliation: Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
authors.forename: Barbora
authors.initials: B
authors.lastname: Malecova
authors.affiliation: Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Dall'Agnese
authors.affiliation: Fondazione Santa Lucia - Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Madaro
authors.affiliation: Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
authors.forename: Sole
authors.initials: S
authors.lastname: Gatto
authors.affiliation: Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
authors.forename: Paula
authors.initials: P
authors.lastname: Coutinho Toto
authors.affiliation: Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
authors.forename: Sonia
authors.initials: S
authors.lastname: Albini
authors.affiliation: Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
authors.forename: Tammy
authors.initials: T
authors.lastname: Ryan
authors.affiliation: Cellular Signaling and Nuclear Dynamics Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire, CU de Strasbourg, France.
authors.forename: Làszlò
authors.identifier: http://orcid.org/0000-0001-7398-2250
authors.initials: L
authors.lastname: Tora
authors.affiliation: Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.|Fondazione Santa Lucia - Istituto di Ricovero e Cura a Carattere Scientifico, Rome, Italy.
authors.forename: Pier Lorenzo
authors.identifier: http://orcid.org/0000-0003-4964-0095
authors.initials: PL
authors.lastname: Puri
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D051379:Mice; D018345:Mice, Knockout; D032342:Muscle Cells / Q000502:physiology*; D017570:MyoD Protein / Q000378:metabolism*; D035181:TATA-Box Binding Protein / Q000378:metabolism*
chemical_list: D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D035181:TATA-Box Binding Protein
keywords: TBP; TFIID; cell biology; chromosomes; genes; human; mouse; muscle differentiation; muscle regeneration; myoD; transcription