title: Microbiological mechanism of the improved nitrogen and phosphorus removal by embedding microbial fuel cell in Anaerobic-Anoxic-Oxic wastewater treatment process.
abstract: Anaerobic-Anoxic-Oxic (AA/O) wastewater treatment process is a widely used wastewater treatment process for simultaneous nitrogen and phosphorus removal. Microbial fuel cell (MFC) can generate electricity and treat the organic wastewater simultaneously. Our previous research showed that embedding MFC in AA/O wastewater treatment process could enhance the pollutants removal efficiency. However, the mechanism was not clear. In this study, a lab-scale corridor-style AA/O reactor with MFC embedded was operated and both the total nitrogen and total phosphorus removal efficiencies were enhanced. DGGE and Illumina Miseq results demonstrated that both the microbial community structures on the surface of the cathode and in the suspensions of cathode chamber have been changed. The percentage of Thauera and Emticicia, identified as denitrifying bacteria, increased significantly in the suspension liquid when the MFC was embedded in the AA/O reactor. Moreover, the genus Rheinheimera were significantly enriched on the cathode surface, which might contribute to both the nitrogen removal enhancement and electricity generation.
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Institution of Environmental Biology and Life Support Technology, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Beizhen
authors.initials: B
authors.lastname: Xie
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Institution of Environmental Biology and Life Support Technology, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Bojie
authors.initials: B
authors.lastname: Liu
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Institution of Environmental Biology and Life Support Technology, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yi
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Institution of Environmental Biology and Life Support Technology, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing 100191, China.
authors.forename: Lige
authors.initials: L
authors.lastname: Yang
authors.affiliation: Beijing Key Laboratory of Bio-inspired Energy Materials and Devices, Beihang University, Beijing 100191, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Liang
authors.affiliation: Key Laboratory of Bio-inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Institution of Environmental Biology and Life Support Technology, Beihang University, Beijing 100191, China; International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing 100191, China. Electronic address: LH64@buaa.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D018441:Biofilms; D019149:Bioreactors; D004247:DNA / Q000737:chemistry; D058440:Denitrification; D004560:Electricity; D004566:Electrodes; D000860:Hypoxia; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen / Q000737:chemistry*; D010758:Phosphorus / Q000737:chemistry*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D020628:Thauera / Q000378:metabolism*; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000737:chemistry; D018508:Water Purification
chemical_list: D004247:DNA; D009584:Nitrogen; D010758:Phosphorus; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: AA/O; DGGE; Illumina Miseq; Microbial fuel cell