title: Degradation kinetics and metabolites in continuous biodegradation of isoprene.
abstract: The kinetic parameters of isoprene biodegradation were studied in a bioreactor, comprising of bioscrubber and polyurethane foam packed biofilter in series and inoculated with Pseudomonas sp., using a Michaelis-Menten type model. The maximum elimination capacity, ECmax; substrate constant, Ks and ECmax/Ks values for bioscrubber were found to be 666.7 g m(-3) h(-1), 9.86 g m(-3) and 67.56 h(-1), respectively while those for biofilter were 3333 g m(-3) h(-1), 13.96 g m(-3) and 238.7 h(-1), respectively. The biofilter section exhibited better degradation efficiency compared to the bioscrubber unit. Around 62-75% of the feed isoprene got converted to carbon dioxide, indicating the efficient capability of bacteria to mineralize isoprene. The FTIR and GC-MS analyses of degradation products indicated oxidative cleavage of unsaturated bond of isoprene. These results were used for proposing a plausible degradation pathway for isoprene.
authors.affiliation: Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Navnita
authors.initials: N
authors.lastname: Srivastva
authors.affiliation: Department of Chemical Engineering and Technology, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ram S
authors.initials: RS
authors.lastname: Singh
authors.affiliation: Department of Chemical Engineering and Technology, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Siddh N
authors.initials: SN
authors.lastname: Upadhyay
authors.affiliation: Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: skdubey@bhu.ac.in.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Dubey
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors; D002070:Butadienes / Q000378:metabolism*; D002244:Carbon / Q000032:analysis; D005374:Filtration / Q000295:instrumentation; D008401:Gas Chromatography-Mass Spectrometry; D045782:Hemiterpenes / Q000378:metabolism*; D007700:Kinetics; D055442:Metabolome; D010420:Pentanes / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D002244:Carbon
keywords: Biofilter; Bioscrubber; GC–MS; Isoprene; Kinetics