title: Microbial degradation of N-methyl-2-pyrrolidone in surface water and bacteria responsible for the process.
abstract: Due to widespread utilization in many industrial spheres and agrochemicals, N-methyl-2-pyrrolidone (NMP) is a potential contaminant of different surface water ecosystems. Hence, investigation was made into its aerobic microbial degradability in samples of water from a river, wetland area and spring. The results showed that the compound was degradable in all water types, and that the fastest NMP removal occurred in 4 days in river water, while in the wetland and spring samples the process was relatively slow, requiring several months to complete. Key bacterial degraders were successfully isolated in all cases, and their identification proved that pseudomonads played a major role in NMP degradation in river water, while the genera Rhodococcus and Patulibacter fulfilled a similar task in the wetland sample. Regarding spring water, degrading members of the Mesorhizobium and Rhizobium genera were found.
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 762 72 Zlín, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Růžička
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 762 72 Zlín, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Jana
authors.initials: J
authors.lastname: Fusková
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 762 72 Zlín, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Karel
authors.initials: K
authors.lastname: Křížek
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 762 72 Zlín, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Markéta
authors.initials: M
authors.lastname: Měrková
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 762 72 Zlín, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Alena
authors.initials: A
authors.lastname: Černotová
authors.affiliation: Department of Environmental Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 762 72 Zlín, Czech Republic E-mail: ruzickaj@ft.utb.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Smělík
mesh_terms: D039903:Actinobacteria / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D018153:Czech Republic; D060985:Natural Springs / Q000032:analysis* / Q000382:microbiology; D011548:Pseudomonadaceae / Q000378:metabolism; D011760:Pyrrolidinones / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism; D045483:Rivers / Q000737:chemistry* / Q000382:microbiology; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D053833:Wetlands
chemical_list: D011760:Pyrrolidinones; D014874:Water Pollutants, Chemical; C038678:N-methylpyrrolidone