title: Detection and confirmation of serum lipid biomarkers for preeclampsia using direct infusion mass spectrometry.
abstract: Despite substantial research, the early diagnosis of preeclampsia remains elusive. Lipids are now recognized to be involved in regulation and pathophysiology of some disease. Shotgun lipidomic studies were undertaken to determine whether serum lipid biomarkers exist that predict preeclampsia later in the same in pregnancy. A discovery study was performed using sera collected at 12-14 weeks pregnancy from 27 controls with uncomplicated pregnancies and 29 cases that later developed preeclampsia. Lipids were extracted and analyzed by direct infusion into a TOF mass spectrometer. MS signals, demonstrating apparent differences were selected, their abundances determined, and statistical differences tested. Statistically significant lipid markers were reevaluated in a second confirmatory study having 43 controls and 37 preeclampsia cases. Multi-marker combinations were developed using those lipid biomarkers confirmed in the second study. The initial study detected 45 potential preeclampsia markers. Of these, 23 markers continued to be statistically significant in the second confirmatory set. Most of these markers, representing several lipid classes, were chemically characterized, typically providing lipid class and potential molecular components using MS(2) Several multi-marker panels with areas under the curve >0.85 and high predictive values were developed. Developed panels of serum lipidomic biomarkers appear to be able to identify most women at risk for preeclampsia in a given pregnancy at 12-14 weeks gestation.
authors.affiliation: Departments of Chemistry and Biochemistry, Brigham Young University, Provo, UT.
authors.forename: Swati
authors.initials: S
authors.lastname: Anand
authors.affiliation: Departments of Chemistry and Biochemistry, Brigham Young University, Provo, UT.
authors.forename: SydneyA
authors.initials: S
authors.lastname: Young
authors.affiliation: Maternal Fetal Medicine, Intermountain Healthcare, Salt Lake City, UT Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT.
authors.forename: M Sean
authors.initials: MS
authors.lastname: Esplin
authors.affiliation: Statistics, Brigham Young University, Provo, UT.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Peaden
authors.affiliation: Statistics, Brigham Young University, Provo, UT.
authors.forename: H Dennis
authors.initials: HD
authors.lastname: Tolley
authors.affiliation: Maternal Fetal Medicine, Intermountain Healthcare, Salt Lake City, UT Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT.
authors.forename: T Flint
authors.initials: TF
authors.lastname: Porter
authors.affiliation: Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT Maternal Fetal Medicine, Intermountain Healthcare, Salt Lake City, UT.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Varner
authors.affiliation: Department of Obstetrics and Gynecology, Columbia University School of Medicine, New York, NY.
authors.forename: Mary E
authors.initials: ME
authors.lastname: D'Alton
authors.affiliation: Departments of Chemistry and Biochemistry, Brigham Young University, Provo, UT.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Jackson
authors.affiliation: Departments of Chemistry and Biochemistry, Brigham Young University, Provo, UT swgraves@chem.byu.edu.
authors.forename: Steven W
authors.initials: SW
authors.lastname: Graves
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D008055:Lipids
keywords: diagnosis; lipidomics; lipids; mass spectrometer; pregnancy