title: Over-expression of survivin is a factor responsible for differential responses of ovarian cancer cells to S-allylmercaptocysteine (SAMC).
abstract: While investigating the inhibitory effect of S-allylmercaptocysteine (SAMC), a garlic derivative, on ovarian cancer, we subjected three ovarian cancer cell lines, HO8910, HO8910PM, and SKOV3, to SAMC treatment. In vivo and in vitro experiments showed that only HO8910 and SKOV3 cells were highly sensitive to SAMC, whereas HO8910PM cells were resistant to SAMC. Subsequently, we examined the apoptosis-related genes in the three cell lines. We found that survivin gene was highly expressed in HO8910PM cells. Down regulation of survivin gene in HO8910PM cells with small interference RNA (siRNA), resulted in increased sensitivity to SAMC together with a decrease in invasiveness of tumor cells. We therefore concluded that the S-allylmercaptocysteine suppresses both the proliferation and distant metastasis of epithelial ovarian cancer cells, insensitivity of HO8910PM cells to SAMC was closely related to the high level of survivin expression and that combination of SAMC treatment together with survivin knockdown might be a potential strategy for treatment of certain variants of ovarian cancers.
authors.affiliation: Department of Thoracic Surgery, the Third Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of General surgery, the First Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Qu
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, China.
authors.forename: Shuwan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, China.
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Cancer Biology Group, Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong.
authors.forename: Yongchuan
authors.initials: Y
authors.lastname: Wong
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, China; Department of Forensic, Harbin Medical University, Harbin, China. Electronic address: chenhe_2008@aliyun.com.
authors.forename: He
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005737:Garlic / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D019012:Integrin beta1 / Q000378:metabolism; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D014410:Tumor Stem Cell Assay; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C107489:BIRC5 protein, human; D015820:Cadherins; D051034:Inhibitor of Apoptosis Proteins; D019012:Integrin beta1; C086300:S-allylmercaptocysteine; D000077022:Survivin; D053148:Caspase 3; D003545:Cysteine
keywords: Drug resistance; E-cadherin; Integrin β1; Ovarian cancer; S-allylmercaptocysteine; Surviving