title: Design of Synthetic Polymer Nanoparticles That Facilitate Resolubilization and Refolding of Aggregated Positively Charged Lysozyme.
abstract: Designed polymer hydrogel nanoparticles (NPs) capable of facilitating resolubilization and refolding of an aggregated protein, positively charged lysozyme, are prepared. NPs designed to interact strongly with denatured lysozyme and relatively weakly with native lysozyme, facilitated resolubilization and refolding of aggregated lysozyme. Such NPs could be prepared by copolymerizing optimized combinations and populations of functional monomers. The refolded lysozyme showed native conformation and enzymatic activity. Eleven grams of aggregated protein was refolded by 1 g of NPs. However, NPs having low affinity to denatured lysozyme and NPs having high affinity to both denatured and native lysozyme showed relatively low facilitation activity. Our results suggest a potential strategy for the design of artificial chaperones with high facilitating activity.
authors.affiliation: Department of Chemical Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nakamoto
authors.affiliation: Department of Chemical Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nonaka
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697, United States.
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Shea
authors.affiliation: Department of Chemical Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Miura
authors.affiliation: Department of Chemical Engineering, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hoshino
mesh_terms: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D018832:Molecular Chaperones / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011489:Protein Denaturation; D017510:Protein Folding
chemical_list: D018832:Molecular Chaperones; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D009113:Muramidase