title: Superovulation in wood bison (Bison bison athabascae): Effects of progesterone, treatment protocol and gonadotropin preparations for the induction of ovulation.
abstract: Experiments were done to determine the ovarian response and embryo production following superstimulation of wood bison. In Experiment 1 (Anovulatory season), the efficacy of pLH vs. hCG for inducing ovulation was compared in wood bison superstimulated with a single dose of pFSH in 0.5% hyaluronan and the effect of exogenous progesterone (PRID) on superovulatory response and embryo quality was examined. In Experiment 2 (Ovulatory season), the efficacy of pLH vs. hCG for the induction of ovulation was compared in wood bison superstimulated with pFSH in a single intramuscular dose vs. a two-dose regimen 48 h apart (split dose) in 0.5% hyaluronan. In Experiment 1, the number of CL was greater (P < 0.05) in bison treated with hCG than pLH (6.6 ± 1.8 vs. 2.8 ± 0.8) and in those that were not given PRID (6.0 ± 1.5 vs. 2.7 ± 1.0). There was no effect of progesterone treatment on embryo quality. In Experiment 2, the number of CL was greater (P < 0.05) in bison treated with hCG than with pLH (6.3 ± 0.8 vs. 3.8 ± 1.2) and in bison superstimulated with split dose vs. single dose of FSH (7.1 ± 0.9 vs. 3.0 ± 0.8). The number of ova/embryos and freezable embryos did not differ among groups in either experiment. In conclusion, hCG induced a greater ovulatory response than pLH in both seasons. Two doses of FSH induced the greatest superovulatory response during the ovulatory season. Exogenous progesterone did not improve embryo quality during the anovulatory season.
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada. Electronic address: jmanuel.palomino@usask.ca.
authors.forename: J Manuel
authors.initials: JM
authors.lastname: Palomino
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada. Electronic address: miriam.cervantes@usask.ca.
authors.forename: Miriam P
authors.initials: MP
authors.lastname: Cervantes
authors.affiliation: Faculty of Veterinary Medicine, University of Calgary, Alberta, Canada. Electronic address: rbmccork@ucalgary.ca.
authors.forename: Robert B
authors.initials: RB
authors.lastname: McCorkell
authors.affiliation: Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada. Electronic address: reuben.mapletoft@usask.ca.
authors.forename: Reuben J
authors.initials: RJ
authors.lastname: Mapletoft
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada. Electronic address: gregg.adams@usask.ca.
authors.forename: Gregg P
authors.initials: GP
authors.lastname: Adams
mesh_terms: D000818:Animals; D016164:Bison / Q000502:physiology*; D004359:Drug Therapy, Combination; D056727:Endangered Species; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006062:Gonadotropins / Q000008:administration & dosage / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D011374:Progesterone / Q000494:pharmacology*; D012621:Seasons
chemical_list: D006062:Gonadotropins; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: FSH; Hyaluronan; Progesterone; Superovulation; Wood bison; hCG; pLH