title: Ecological distribution of leaf stomata and trichomes among tree species in a Malaysian lowland tropical rain forest.
abstract: The vertical structure of a tropical rain forest is complex and multilayered, with strong variation of micro-environment with height up to the canopy. We investigated the relation between morphological traits of leaf surfaces and tree ecological characteristics in a Malaysian tropical rain forest. The shapes and densities of stomata and trichomes on the abaxial leaf surfaces and their relation with leaf characteristics such as leaf area and leaf mass per area (LMA) were studied in 136 tree species in 35 families with different growth forms in the tropical moist forest. Leaf physiological properties were also measured in 50 canopy and emergent species. Most tree species had flat type (40.4 %) or mound type (39.7 %) stomata. In addition, 84 species (61.76 %) in 22 families had trichomes, including those with glandular (17.65 %) and non-glandular trichomes (44.11 %). Most leaf characteristics significantly varied among the growth form types: species in canopy and emergent layers and canopy gap conditions had higher stomatal density, stomatal pore index (SPI), trichome density and LMA than species in understory and subcanopy layers, though the relation of phylogenetically independent contrasts to each characteristic was not statistically significant, except for leaf stomatal density, SPI and LMA. Intrinsic water use efficiency in canopy and emergent tree species with higher trichome densities was greater than in species with lower trichome densities. These results suggest that tree species in tropical rain forests adapt to a spatial difference in their growth forms, which are considerably affected by phylogenetic context, by having different stomatal and trichome shapes and/or densities.
authors.affiliation: Faculty of Agriculture, Kochi University, B200, Monobe, Nankoku, 783-8502, Japan. ichie@kochi-u.ac.jp.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ichie
authors.affiliation: The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, 790-8566, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Faculty of Agriculture, Kochi University, B200, Monobe, Nankoku, 783-8502, Japan.
authors.forename: Narumi
authors.initials: N
authors.lastname: Takahashi
authors.affiliation: Faculty of Agriculture, Ehime University, Matsuyama, 790-8566, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kamiya
authors.affiliation: Forestry and Forest Products Research Institute, Tsukuba, 305-8687, Japan.
authors.forename: Tanaka
authors.initials: T
authors.lastname: Kenzo
mesh_terms: D000704:Analysis of Variance; D008296:Malaysia; D010788:Photosynthesis; D010802:Phylogeny; D054046:Plant Stomata / Q000502:physiology* / Q000648:ultrastructure; D065947:Rainforest; D013045:Species Specificity; D014197:Trees / Q000033:anatomy & histology / Q000502:physiology*; D064201:Trichomes / Q000502:physiology* / Q000648:ultrastructure
keywords: Growth form type; Intrinsic water use efficiency; Phylogenetic constraints; Stomatal elevation; Trichome; Tropical rain forest