title: Evaluation of the effectiveness of manual and automated dialyzers reprocessing after multiple reuses.
abstract: A cross-sectional study was conducted to evaluate the effectiveness of manual and automated dialyzer reprocessing. Dialyzers were filled with fluid thioglycollate medium from blood and dialysate chambers after being reprocessed and chemically sterilized with 0.2% peracetic acid. They were incubated for 14 days at 35°C ± 2°C, and microbiologic analysis was performed. Microorganisms were identified in 3 of the 11 samples (27.3%) from the blood chambers: Sphingomonas paucimobilis (2/3) and Penicillium spp (1/3) and in 11 of the 11 samples (100%) from the dialysate chambers: S paucimobilis (7/11), Stenotrophomonas maltophilia (4/11), Pseudomonas aeruginosa (3/11), Candida spp (1/11), and Acinetobacter baumannii (1/11). Of the 4 manually reprocessed dialyzers, gram-positive bacillus were identified in 1 sample (25%) from the blood chamber, and Bacillus spp and Burkholderia spp were identified in 1 sample (25%) from the dialysate chamber. The dialyzers reprocessing can pose risks safety because of exposure patient to microorganisms.
authors.affiliation: School of Nursing University of São Paulo, São Paulo, SP, Brazil; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil. Electronic address: alexandra.toniolo@einstein.br.
authors.forename: Alexandra do Rosário
authors.initials: Ado R
authors.lastname: Toniolo
authors.affiliation: School of Nursing, Federal University of Minas Gerais, Belo Horizonte, Brazil; University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Maíra Marques
authors.initials: MM
authors.lastname: Ribeiro
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Ishii
authors.affiliation: Laboratory of Microbiology, Service of Hospital Infection, Irmandade Santa Casa de Misericordiade Sao Paulo, São Paulo, Brazil.
authors.forename: Cely Barreto
authors.initials: CB
authors.lastname: da Silva
authors.affiliation: Department of Hospital Infection Control, Irmandade Santa Casa de Misericordia de São Paulo, São Paulo, SP, Brazil.
authors.forename: Lycia Mara
authors.initials: LM
authors.lastname: Jenné Mimica
authors.affiliation: School of Nursing, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Kazuko Uchikawa
authors.initials: KU
authors.lastname: Graziano
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D002175:Candida / Q000302:isolation & purification*; D003430:Cross-Sectional Studies; D004202:Disinfectants / Q000008:administration & dosage; D004203:Disinfection / Q000379:methods*; D017743:Equipment Reuse; D007688:Kidneys, Artificial / Q000382:microbiology*; D010463:Peracetic Acid / Q000008:administration & dosage
chemical_list: D004202:Disinfectants; D010463:Peracetic Acid
keywords: Hemodialyzer; reuse; sterilization