title: Preparation of phenylboronate affinity rigid monolith with macromolecular porogen.
abstract: Boronate-affinity monolithic column was first prepared via polystyrene (PS) as porogen in this work. The monolithic polymer was synthetized using 4-vinylphenylboronic acid (4-VPBA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as crosslinker monomer, and a mixture of PS solution in tetrahydrofuran, the linear macromolecular porogen, and toluene as porogen. Isoquercitrin (ISO) and hyperoside (HYP), isomer diol flavonoid glycosides, can be baseline separated on the poly(VPBA-co-EDMA) monolith. The effect of polymerization variables on the selectivity factor, e.g., the ratio of monomer to crosslinker (M/C), the amount of PS and the molecular weight of macromolecular porogen was investigated. The surface properties of the monolithic polymer were characterized by scanning electron microscopy and nitrogen adsorption. The best polymerization condition was the M/C ratio of 7:3, and the PS concentration of 40 mg/ml. The poly(VPBA-co-EDMA) polymer was also applied to extract cis-diol flavonoid glycosides from the crude extraction of cotton flower. After treated by poly(VPBA-co-EDMA) for solid phase extraction, high purity ISO and HYP (>99.96%) can be obtained with recovery of 83.7% and 78.6%, respectively.
authors.affiliation: Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China; State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Xiang-Jie
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China; State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Man
authors.initials: M
authors.lastname: Jia
authors.affiliation: Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhao
authors.affiliation: Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China; State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. Electronic address: zhaoshengliu@sohu.com.
authors.forename: Zhao-Sheng
authors.initials: ZS
authors.lastname: Liu
authors.affiliation: Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China; State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. Electronic address: haji@ms.xjb.ac.cn.
authors.forename: Haji
authors.initials: H
authors.lastname: Akber Aisa
mesh_terms: D000327:Adsorption; D001897:Boronic Acids / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D008970:Molecular Weight; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D001897:Boronic Acids; D046911:Macromolecular Substances; D008689:Methacrylates; D011108:Polymers; D011137:Polystyrenes; C004919:ethylene dimethacrylate; C010686:benzeneboronic acid
keywords: Flavonol glycosides; HPLC; Macromolecular porogen; Monolithic column; Phenylboronate affinity monolith; Solid phase extraction