title: Integrated chemical and multi-scale structural analyses for the processes of acid pretreatment and enzymatic hydrolysis of corn stover.
abstract: Corn stover was pretreated with acid under moderate conditions (1.5%, w/w, 121°C, 60min), and kinetic enzymolysis experiments were performed on the pretreated substrate using a mixture of Celluclast 1.5L (20FPU/g dry substrate) and Novozyme 188 (40CBU/g dry substrate). Integrated chemical and multi-scale structural methods were then used to characterize both processes. Chemical analysis showed that acid pretreatment removed considerable hemicellulose (from 19.7% in native substrate to 9.28% in acid-pretreated substrate) and achieved a reasonably high conversion efficiency (58.63% of glucose yield) in the subsequent enzymatic hydrolysis. Multi-scale structural analysis indicated that acid pretreatment caused structural changes via cleaving acetyl linkages, solubilizing hemicellulose, relocating cell wall surfaces and enlarging substrate porosity (pore volume increased from 0.0067cm(3)/g in native substrate to 0.019cm(3)/g in acid-pretreated substrate), thereby improving the polysaccharide digestibility.
authors.affiliation: College of Engineering, China Agricultural University (East Campus), 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing 100083, PR China. Electronic address: clj1020@cau.edu.cn.
authors.forename: Longjian
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Engineering, China Agricultural University (East Campus), 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing 100083, PR China. Electronic address: lijunbao0702@cau.edu.cn.
authors.forename: Junbao
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Engineering, China Agricultural University (East Campus), 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing 100083, PR China. Electronic address: bibilailms@cau.edu.cn.
authors.forename: Minsheng
authors.initials: M
authors.lastname: Lu
authors.affiliation: College of Engineering, China Agricultural University (East Campus), 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing 100083, PR China. Electronic address: xmguo_914@cau.edu.cn.
authors.forename: Xiaomiao
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Engineering, China Agricultural University (East Campus), 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing 100083, PR China. Electronic address: zhanghaiyan@cau.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Engineering, China Agricultural University (East Campus), 17 Qing-Hua-Dong-Lu, Hai-Dian District, Beijing 100083, PR China. Electronic address: clj1020@googlemail.com.
authors.forename: Lujia
authors.initials: L
authors.lastname: Han
mesh_terms: D000143:Acids / Q000737:chemistry*; D000821:Animal Feed; D055162:Biocatalysis; D005947:Glucose / Q000737:chemistry; D006358:Hot Temperature; D006868:Hydrolysis; D011134:Polysaccharides / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000143:Acids; D011134:Polysaccharides; C007916:hemicellulose; D005947:Glucose
keywords: Acid pretreatment; Chemical analysis; Corn stover; Enzymatic hydrolysis; Structural analysis