title: High-throughput-sequencing-based identification of a grapevine fanleaf virus satellite RNA in Vitis vinifera.
abstract: A new satellite RNA (satRNA) of grapevine fanleaf virus (GFLV) was identified by high-throughput sequencing of high-definition (HD) adapter libraries from grapevine plants of the cultivar Panse precoce (PPE) affected by enation disease. The complete nucleotide sequence was obtained by automatic sequencing using primers designed based on next-generation sequencing (NGS) data. The full-length sequence, named satGFLV-PPE, consisted of 1119 nucleotides with a single open reading frame from position 15 to 1034. This satRNA showed maximum nucleotide sequence identity of 87 % to satArMV-86 and satGFLV-R6. Symptomatic grapevines were surveyed for the presence of the satRNA, and no correlation was found between detection of the satRNA and enation symptom expression.
authors.affiliation: CNR Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, 70126, Bari, Italy. michela.chiumenti@ipsp.cnr.it.
authors.forename: Michela
authors.initials: M
authors.lastname: Chiumenti
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR47TJ, United Kingdom.
authors.forename: Irina
authors.initials: I
authors.lastname: Mohorianu
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti (Di.S.S.P.A.), Università degli Studi di Bari Aldo Moro, 70126, Bari, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Roseti
authors.affiliation: CNR Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, 70126, Bari, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Saldarelli
authors.affiliation: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR47TJ, United Kingdom.
authors.forename: Tamas
authors.initials: T
authors.lastname: Dalmay
authors.affiliation: CNR Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, 70126, Bari, Italy.
authors.forename: Angelantonio
authors.initials: A
authors.lastname: Minafra
mesh_terms: D001483:Base Sequence; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D017860:Nepovirus / Q000235:genetics*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D019244:RNA, Satellite / Q000235:genetics*; D027843:Vitis / Q000821:virology*
chemical_list: D019244:RNA, Satellite