title: Histone acetyltransferease p300 modulates TIM4 expression in dendritic cells.
abstract: TIM4 (T cell immunoglobulin mucin domain molecule-4) plays a critical role in the initiation of skewed T helper (Th) 2 polarization. The factors regulating TIM4 expression are unclear. This study tests a hypothesis that p300 and STAT6 (signal transducer and activator transcription-6) regulates TIM4 expression in dendritic cells (DC). In this study, a food allergy mouse model was developed with ovalbumin (a specific antigen) and cholera toxin (CT; an adjuvant). The chromatin immunoprecipitation assay was performed to evaluate the chromatin changes at TIM4 and STAT6 promoters. The TIM4 expression was evaluated by real time RT-PCR and Western blotting. The results showed that high levels of p300 and TIM4 were detected in the intestinal DCs of mice with intestinal allergy. p300 is involved in the CT-induced TIM4 expression in DCs. p300 interacts with the chromatin at the TIM4 promoter locus in DCs isolated from allergic mice. CT increases p300 expression to regulate STAT6 levels in DCs. STAT6 mediates the CT-induced TIM4 expression in DCs. In conclusion, p300 and STAT6 mediate the microbial product CT-induced TIM4 expression in DCs.
authors.affiliation: The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.|Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pathology &Molecular Medicine, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Lin-Jing
authors.initials: LJ
authors.lastname: Li
authors.affiliation: The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Xu
authors.affiliation: Department of Pathology &Molecular Medicine, McMaster University, Hamilton, ON, Canada L8N 4A6.|ENT Institute, Longgang Central Hospital, Shenzhen 518116, China.
authors.forename: Jiang-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Department of Pathology &Molecular Medicine, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Huan-Ping
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: ENT Institute, Longgang Central Hospital, Shenzhen 518116, China.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Geng
authors.affiliation: The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: The Center of Allergy &Immunology, Shenzhen University School of Medicine, Shenzhen, 518060, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000818:Animals; D016764:Cell Polarity / Q000276:immunology; D002772:Cholera Toxin / Q000009:adverse effects / Q000276:immunology / Q000494:pharmacology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000276:immunology; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D010047:Ovalbumin / Q000009:adverse effects / Q000276:immunology / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D050800:STAT6 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology; D050880:p300-CBP Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D000099224:p300-CBP-Associated Factor
chemical_list: D002772:Cholera Toxin; D008565:Membrane Proteins; D010047:Ovalbumin; D050800:STAT6 Transcription Factor; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C494100:Stat6 protein, mouse; C500399:TIM-4 protein, mouse