title: MicroRNA-132 cause apoptosis of glioma cells through blockade of the SREBP-1c metabolic pathway related to SIRT1.
abstract: BACKGROUND
  The inhibition role of miRNA (microRNA or miR) on cancer signaling pathways has been used to prospective cancer treatment. SIRT1 might promote tumorigenesis in human glioma.
  
  
  METHODS
  Here, we investigated whether miR-132 regulate the expression of SIRT1 and its downstream SREBP (Sterol regulatory element-binding protein)-lipogenesis-cholesterogenesis metabolic pathway in human glioma cells. Furthermore, we studied the effect on biology function of glioma cell induced by miR-132.
  
  
  RESULTS
  MiR-132 inhibited SIRT1 and SREBP-1c expression and downregulated their targeted genes, including HMGCR and FASN. MiR-132 suppressed the cell growth, tumorigenicity, the invasion of glioma cells and migration as well as promoted their apoptosis. The pathways associated with cancer progression and tumorigenicity, and induce glioma cell apoptosis has been inhibited by miR-132 involving in a caspase-dependent apoptotic mechanism.
  
  
  CONCLUSIONS
  The recovery of miR-132 resulted in caspase-dependent apoptotic death in glioma cells. MiR-132 that was newly discovered represents a newly targeting mechanism in treatment for glioma.
authors.affiliation: Medical School of Chinese PLA, Beijing 100853, People's Republic of China; Department of Neurosurgery, Bayi brain hospital affiliated to general hospital of Beijing military of PLA, Beijing 100070, People's Republic of China.
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, the Second People's Hospital of Guangdong province, Southern Medical University, Guangzhou 510515, People's Republic of China. Electronic address: jingwanghou@163.com.
authors.forename: Jinqian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Surgery, the Second People's Hospital of Guangdong province, Southern Medical University, Guangzhou 510515, People's Republic of China.
authors.forename: Jingliang
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Surgery, the Second People's Hospital of Guangdong province, Southern Medical University, Guangzhou 510515, People's Republic of China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Surgery, the Second People's Hospital of Guangdong province, Southern Medical University, Guangzhou 510515, People's Republic of China.
authors.forename: Guoan
authors.initials: G
authors.lastname: Xiang
authors.affiliation: Medical School of Chinese PLA, Beijing 100853, People's Republic of China; Department of Neurosurgery, Bayi brain hospital affiliated to general hospital of Beijing military of PLA, Beijing 100070, People's Republic of China. Electronic address: jianshengliylp@163.com.
authors.forename: Ruxiang
authors.initials: R
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002784:Cholesterol / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D054890:Fatty Acid Synthase, Type I / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; C547077:MIRN132 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D002784:Cholesterol; C553220:HMGCR protein, human; D006903:Hydroxymethylglutaryl CoA Reductases; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I; D056564:Sirtuin 1
keywords: Apoptosis; Glioma; Lipid metabolism; SIRT1; SREBP-1c; microRNA