title: Removal of Polysorbate 80 by complexation prior to LC-MS analysis.
abstract: The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.
authors.affiliation: Analytical Chemistry Group, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Copenhagen, Denmark. dkkba@chr-hansen.com.|Chr. Hansen A/S, Innovation & Assays, Hørsholm, Denmark. dkkba@chr-hansen.com.
authors.forename: Kristina B
authors.initials: KB
authors.lastname: Jäpelt
authors.affiliation: Chr. Hansen A/S, Innovation & Assays, Hørsholm, Denmark.|MS-Omics, Thorvaldsensvej 40, T644, Frederiksberg, 1871, Denmark.
authors.forename: Lea Giørtz
authors.initials: LG
authors.lastname: Johnsen
authors.affiliation: Analytical Chemistry Group, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jan H
authors.initials: JH
authors.lastname: Christensen
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D008970:Molecular Weight; D011136:Polysorbates / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D011136:Polysorbates; D013861:Thiocyanates
keywords: Detergent; Liquid chromatography; Mass spectrometry; Metabolite analysis; Tween 80