title: Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain.
abstract: Real-time activity measurements from multiple specific cell populations and projections are likely to be important for understanding the brain as a dynamical system. Here we developed frame-projected independent-fiber photometry (FIP), which we used to record fluorescence activity signals from many brain regions simultaneously in freely behaving mice. We explored the versatility of the FIP microscope by quantifying real-time activity relationships among many brain regions during social behavior, simultaneously recording activity along multiple axonal pathways during sensory experience, performing simultaneous two-color activity recording, and applying optical perturbation tuned to elicit dynamics that match naturally occurring patterns observed during behavior.
authors.affiliation: Neurosciences Program, Stanford University, Stanford, California, USA.
authors.forename: Christina K
authors.initials: CK
authors.lastname: Kim
authors.affiliation: Department of Electrical Engineering, Stanford University, Stanford, California, USA.
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Yang
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Nandini
authors.initials: N
authors.lastname: Pichamoorthy
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Noah P
authors.initials: NP
authors.lastname: Young
authors.affiliation: Department of Electrical Engineering, Stanford University, Stanford, California, USA.
authors.forename: Isaac
authors.initials: I
authors.lastname: Kauvar
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Joshua H
authors.initials: JH
authors.lastname: Jennings
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Talia N
authors.initials: TN
authors.lastname: Lerner
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Andre
authors.initials: A
authors.lastname: Berndt
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Soo Yeun
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Charu
authors.initials: C
authors.lastname: Ramakrishnan
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Davidson
authors.affiliation: Department of Neurochemistry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.|Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Department of Neurochemistry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.|Core Research for Evolutional Science and Technology, Japan Agency for Medical Research and Development, Tokyo, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Bito
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA.|Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.|Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, California, USA.
authors.forename: Karl
authors.initials: K
authors.lastname: Deisseroth
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D001931:Brain Mapping / Q000379:methods*; D020013:Calcium Signaling; D051379:Mice; D009434:Neural Pathways; D010783:Photometry / Q000379:methods*; D012919:Social Behavior