title: Evidence for possible role of toll-like receptor 3 mediating virus-induced progression of pituitary adenomas.
abstract: Tumor-related viruses are known to be involved in initiation and progression of certain tumors. However, the relationship between virus and pituitary adenomas (PAs) remains unknown. Here, we investigated infection status of three types of viruses (HPV16, HHV6B and HSV1) and expression level of toll-like receptor 3 (TLR3) in 60 human PA samples. We also determined the role of TLR3 signaling pathway on a PA cell line (GH3). We firstly found that positive rates of HPV16 and HHV6B infection were significantly higher in invasive PA samples than in noninvasive samples (P < 0.01). Similarly, TLR3 mRNA and protein expression also increased in invasive PA samples (P < 0.01). In vitro analysis indicated that GH3 cell proliferation and survival were enhanced by TLR3 activation, which was accompanied by NF-κB activation. Our data indicate that HPV16 and HHV6B viruses may be involved in promoting the progression of PA by activating the TLR3 signaling pathway.
authors.affiliation: Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.affiliation: Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Zhenle
authors.initials: Z
authors.lastname: Zang
authors.affiliation: Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Hu
authors.affiliation: Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: An
authors.affiliation: Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Pei
authors.affiliation: Innovative Drug Research Centre, University of Chongqing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Biology and Biochemistry, College of Natural Sciences and Mathematics, University of Houston, TX, USA. Electronic address: wzhang13@uh.edu.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Multidisciplinary Center for Pituitary Adenomas of Chongqing, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. Electronic address: yanghui64@hotmail.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D015654:Herpesvirus 6, Human / Q000502:physiology; D052162:Human papillomavirus 16 / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000473:pathology; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D051381:Rats; D019349:Roseolovirus Infections / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D051196:Toll-Like Receptor 3 / Q000378:metabolism*
chemical_list: C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3
keywords: Pituitary adenoma; Progression; Toll-like receptor 3; Viruses