title: Engineering Plants for Geminivirus Resistance with CRISPR/Cas9 System.
abstract: The CRISPR/Cas9 system is an efficient genome-editing platform for diverse eukaryotic species, including plants. Recent work harnessed CRISPR/Cas9 technology to engineer resistance to geminiviruses. Here, we discuss opportunities, emerging developments, and potential pitfalls for using this technology to engineer resistance against single and multiple geminivirus infections in plants.
authors.affiliation: Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia; National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
authors.forename: Syed Shan-E-Ali
authors.initials: SS
authors.lastname: Zaidi
authors.affiliation: National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
authors.forename: Shahid
authors.initials: S
authors.lastname: Mansoor
authors.affiliation: Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
authors.forename: Zahir
authors.initials: Z
authors.lastname: Ali
authors.affiliation: Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
authors.forename: Manal
authors.initials: M
authors.lastname: Tashkandi
authors.affiliation: Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. Electronic address: magdy.mahfouz@kaust.edu.sa.
authors.forename: Magdy M
authors.initials: MM
authors.lastname: Mahfouz
mesh_terms: D001709:Biotechnology / Q000379:methods*; D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D017791:Geminiviridae / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology; D010944:Plants / Q000235:genetics* / Q000276:immunology / Q000821:virology; D030821:Plants, Genetically Modified
keywords: CRISPR/Cas9; geminiviruses; genome editing; virus resistance