title: Comparison of gas chromatography-mass spectrometry and gas chromatography-combustion-isotope ratio mass spectrometry analysis for in vivo estimates of metabolic fluxes.
abstract: Gas chromatography-mass spectrometry (GC-MS) was compared with gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) for measurements of cholesterol (13)C enrichment after infusion of labeled precursor ([(13)C1,2]acetate). Paired results were significantly correlated, although GC-MS was less accurate than GC-C-IRMS for higher enrichments. Nevertheless, only GC-MS was able to provide information on isotopologue distribution, bringing new insights to lipid metabolism. Therefore, we assessed the isotopologue distribution of cholesterol in humans and dogs known to present contrasted cholesterol metabolic pathways. The labeled tracer incorporation was different in both species, highlighting the subsidiarity of GC-MS and GC-C-IRMS to analyze in vivo stable isotope studies.
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France.
authors.forename: Mikaël
authors.initials: M
authors.lastname: Croyal
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France.
authors.forename: Raphaëlle
authors.initials: R
authors.lastname: Bourgeois
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France.
authors.forename: Khadija
authors.initials: K
authors.lastname: Ouguerram
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Billon-Crossouard
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Aguesse
authors.affiliation: CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France; Oniris, National College of Veterinary Medicine, Food Science, and Engineering, Nutrition and Endocrinology Unit, F-44307 Nantes, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Nguyen
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France; Endocrinology, G and R Laennec Hospital, F-44093 Nantes, France. Electronic address: michel.krempf@univ-nantes.fr.
authors.forename: Michel
authors.initials: M
authors.lastname: Krempf
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Ferchaud-Roucher
authors.affiliation: INRA, UMR 1280, Physiologie des Adaptations Nutritionnelles, CHU Hôtel-Dieu, F-44000 Nantes, France; CRNH, Human Nutrition Research Center, CHU Hôtel-Dieu, F-44093 Nantes, France; Endocrinology, G and R Laennec Hospital, F-44093 Nantes, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Nobécourt
mesh_terms: D002247:Carbon Isotopes / Q000032:analysis; D002784:Cholesterol / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*
chemical_list: D002247:Carbon Isotopes; D002784:Cholesterol
keywords: Cholesterol enrichment; GC–C–IRMS; GC–MS; Isotopologue; Metabolic flux analysis; [(13)C(1,2)]acetate