title: Biomimetic interconnected porous keratin-fibrin-gelatin 3D sponge for tissue engineering application.
abstract: The medicated wound dressing material with highly interconnected pores, mimicking the function of the extracellular matrix was fabricated for the promotion of cell growth. In this study, keratin (K), fibrin (F) and gelatin (G) composite scaffold (KFG-SPG) was fabricated by freeze drying technique and the mupirocin (D) drug was successfully incorporated with KFG-SPG (KFG-SPG-D) intended for tissue engineering applications. The fabrication of scaffold was performed without the use of any strong chemical solvents, and the solid sponge scaffold was obtained with well interconnected pores. The porous morphology of the scaffold was confirmed by SEM analysis and exhibited competent mechanical properties. KFG-SPG and KFG-SPG-D possess high level of biocompatibility, cell proliferation and cell adhesion of NIH 3T3 fibroblast and human keratinocytes (HaCaT) cell lines thereby indicating the scaffolds potential as a suitable medicated dressing for wound healing.
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamilnadu, India.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Singaravelu
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamilnadu, India.
authors.forename: Giriprasath
authors.initials: G
authors.lastname: Ramanathan
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamilnadu, India.
authors.forename: M D
authors.initials: MD
authors.lastname: Raja
authors.affiliation: Department of Mechanical Engineering, University of Colorado, Boulder, USA.
authors.forename: Naveen
authors.initials: N
authors.lastname: Nagiah
authors.affiliation: Department of Virology, King Institute of Preventive Medicine and Research, Guindy, Chennai 600032, Tamilnadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Padmapriya
authors.affiliation: Department of Virology, King Institute of Preventive Medicine and Research, Guindy, Chennai 600032, Tamilnadu, India.
authors.forename: Krishnasamy
authors.initials: K
authors.lastname: Kaveri
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamilnadu, India. Electronic address: suma67@gmail.com.
authors.forename: Uma Tiruchirapalli
authors.initials: UT
authors.lastname: Sivagnanam
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D065546:Drug Liberation; D004795:Enzyme Stability / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005337:Fibrin / Q000737:chemistry*; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D007633:Keratins / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D016062:Porosity; D013211:Staphylococcus aureus / Q000187:drug effects; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D007633:Keratins; D005780:Gelatin; D005337:Fibrin
keywords: Biocompatibility; Fibrin; Keratin; Tissue engineering; Wound dressing