title: Applications of calcium electroporation to effective apoptosis induction in fibrosarcoma cells and stimulation of normal muscle cells.
abstract: The electroporation (EP) supports various types of anticancer therapies by the selective transport of cytostatics. Increase in intracellular calcium level by EP may be a new approach to fibrosarcoma treatment. Calcium is one of the most important factors of cell proliferation, differentiation and cell death (apoptosis or necrosis). Calcium level balanced by electroporation can cause different effects on normal and pathological cells. The efficiency and safety of electroporation combined with Ca(2+) ions were examined in our study. The two muscle cell lines were used: normal rat skeletal muscle cells - L6 and cancer muscle cells - Wehi-164 (fibrosarcoma). Two CaCl2 concentrations were tested: 0.5 mM and 5 mM combined with EP parameters: 1000 V/cm, 1200 V/cm, and 1500 V/cm. The results show that EP supported by Ca(2+) is cytotoxic for Wehi-164 cells and simultaneously safe for normal muscle cells. The main type of cell death - apoptosis - was confirmed by Tunnel and Annexin V/PI assay. Additionally, sPLA2 pro-tumorigenic influence was proved by immunocytochemistry. Moreover, EP with 0.5 mM of Ca(2+) slightly stimulates the normal muscle cells - L6 to increase proliferation.
authors.affiliation: Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21 St., 50-335 Wroclaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Zielichowska
authors.affiliation: Department of Animal Developmental Biology, Institute of Experimental Biology, University of Wroclaw, Sienkiewicza 21 St., 50-335 Wroclaw, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Daczewska
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10 St., 50-368 Wroclaw, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Saczko
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10 St., 50-368 Wroclaw, Poland.
authors.forename: Olga
authors.initials: O
authors.lastname: Michel
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10 St., 50-368 Wroclaw, Poland. Electronic address: julita.kulbacka@umed.wroc.pl.
authors.forename: Julita
authors.initials: J
authors.lastname: Kulbacka
mesh_terms: D000818:Animals; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism / Q000627:therapeutic use*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018274:Electroporation / Q000379:methods*; D005354:Fibrosarcoma / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D032342:Muscle Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D019042:Muscle Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats
chemical_list: D002118:Calcium
keywords: Apoptosis; Calcium; Electroporation; Fibrosarcoma; Muscle cells; Proliferation