title: Strong and Biostable Hyaluronic Acid-Calcium Phosphate Nanocomposite Hydrogel via in Situ Precipitation Process.
abstract: Hyaluronic acid (HAc) hydrogel exhibits excellent biocompatibility, but it has limited biomedical application due to its poor biomechanical properties as well as too-fast enzymatic degradation. In this study, we have developed an in situ precipitation process for the fabrication of a HAc-calcium phosphate nanocomposite hydrogel, after the formation of the glycidyl methacrylate-conjugated HAc (GMHA) hydrogels via photo-cross-linking, to improve the mechanical and biological properties under physiological conditions. In particular, our process facilitates the rapid incorporation of calcium phosphate (CaP) nanoparticles of uniform size and with minimal agglomeration into a polymer matrix, homogeneously. Compared with pure HAc, the nanocomposite hydrogels exhibit improved mechanical behavior. Specifically, the shear modulus is improved by a factor of 4. The biostability of the nanocomposite hydrogel was also significantly improved compared with that of pure HAc hydrogels under both in vitro and in vivo conditions.
authors.affiliation: Department of Materials Science and Engineering, Seoul National University , Seoul 151-742, Korea.
authors.forename: Seol-Ha
authors.initials: SH
authors.lastname: Jeong
authors.affiliation: School of Biomedical Engineering, Korea University , Seoul 136-703, Korea.
authors.forename: Young-Hag
authors.initials: YH
authors.lastname: Koh
authors.affiliation: Department of Reconstructive and Plastic Surgery, Seoul National University Hospital , Seoul 110-744, Korea.
authors.forename: Suk-Wha
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, Seoul National University Boramae Hospital , Seoul 156-707, Korea.
authors.forename: Ji-Ung
authors.initials: JU
authors.lastname: Park
authors.affiliation: Department of Materials Science and Engineering, Seoul National University , Seoul 151-742, Korea.|Biomedical Implant Convergence Research Center, Advanced Institutes of Convergence Technology , Suwon 443-270, Korea.
authors.forename: Hyoun-Ee
authors.initials: HE
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering, Seoul National University , Seoul 151-742, Korea.|Biomedical Implant Convergence Research Center, Advanced Institutes of Convergence Technology , Suwon 443-270, Korea.
authors.forename: Juha
authors.initials: J
authors.lastname: Song
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000138:chemical synthesis* / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002460:Cell Line; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000009:adverse effects / Q000138:chemical synthesis* / Q000737:chemistry; D051379:Mice; D053761:Nanocomposites / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D020100:Hydrogels; D006820:Hyaluronic Acid; C020243:calcium phosphate