title: Effect of infliximab on acute hepatic ischemia/reperfusion injury in rats.
abstract: This study aimed to investigate the hepatoprotective and antioxidant effects of infliximab (IFX) against liver ischemia/reperfusion (I/R) injury in rats. A total of 30 male Wistar albino rats were divided into three groups: sham, I/R, and I/R+IFX. IFX was given at a dose of 3 mg/kg for three days before I/R. Rat livers were subjected to 60 min of ischemia followed by 90 h of reperfusion. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), TNF-α, malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels were measured in the serum. The liver was removed to evaluate the histopathologic changes. The I/R group had a significant increase in AST, ALT, MDA, and TNF-α levels, and a decrease in GSH-Px activity compared with the sham group. The use of IFX significantly reduced the ALT, AST, MDA and TNF-α levels and significantly increased GSH-Px activity. IFX attenuated the histopathologic changes. IFX has a protective effect on liver I/R injury. This liver protective effect may be related to antioxidant and anti-TNF-α effects. We propose that, for the relief of liver injury subsequent to transplantation, liver resection, trauma, and shock, tentative treatments can be incorporated with IFX, which is already approved for clinical use.
authors.affiliation: Department of Surgery, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Ahmet Fikret
authors.initials: AF
authors.lastname: Yucel
authors.affiliation: Department of Surgery, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Pergel
authors.affiliation: Department of Surgery, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Aydin
authors.affiliation: Department of Biochemistry, School of Medicine, Hacettepe University Ankara, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Alacam
authors.affiliation: Department of Surgery, School of Medicine, Ondokuz Mayıs University Samsun, Turkey.
authors.forename: Ilhan
authors.initials: I
authors.lastname: Karabicak
authors.affiliation: Department of Surgery, School of Medicine, Giresun University Giresun, Turkey.
authors.forename: Tugrul
authors.initials: T
authors.lastname: Kesicioglu
authors.affiliation: Department of Histology and Embryology, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Tumkaya
authors.affiliation: Department of Histology and Embryology, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Yildiray
authors.initials: Y
authors.lastname: Kalkan
authors.affiliation: Department of Surgery, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Ender
authors.initials: E
authors.lastname: Ozer
authors.affiliation: Clinics of Anesthesiology, Erzurum Training and Research Hospital Erzurum, Turkey.
authors.forename: Zakir
authors.initials: Z
authors.lastname: Arslan
authors.affiliation: Department of Pathology, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Sehitoglu
authors.affiliation: Department of Surgery, School of Medicine, Recep Tayyip Erdogan University Rize, Turkey.
authors.forename: Dursun Ali
authors.initials: DA
authors.lastname: Sahin
keywords: Liver; infliximab; ischemia/reperfusion injury; oxidative stress; rat