title: Use of mulberry-soybean intercropping in salt-alkali soil impacts the diversity of the soil bacterial community.
abstract: Diverse intercropping system has been used to control disease and improve productivity in the field. In this research, the bacterial communities in salt-alkali soils of monoculture and intercropping mulberry and soybean were studied using 454-pyrosequencing of the 16S rDNA gene. The dominant taxonomic groups were Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Bacteroidetes, Planctomycetes and Gemmatimonadetes and these were present across all samples. However, the diversity and composition of bacterial communities varied between monoculture and intercropping samples. The estimated bacterial diversity (H') was higher with intercropping soybean than in monoculture soybean, whereas H' showed an opposite pattern in monoculture and intercropping mulberry. Populations of Actinobacteria, Acidobacteria, and Proteobacteria were variable, depending on growth of plants as monoculture or intercropped. Most of Actinobacteria and Chloroflexi were found in intercropping samples, while Acidobacteria and Proteobacteria were present at a higher percentage in monoculture samples. The plant diversity of aboveground and microbial diversity of belowground was linked and soil pH seemed to influence the bacterial community. Finally, the specific plant species was the major factor that determined the bacterial community in the salt-alkali soils.
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Forestry Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Minglong
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Resources and Environment, Northeast Agricultural University, Harbin, Heilongjiang Province, 150030, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Natural Resources and Ecology Institute, Heilongjiang Academy of Sciences, Harbin, 150040, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Xu
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Sun
mesh_terms: D000468:Alkalies / Q000032:analysis*; D058448:Biota; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D031625:Morus / Q000254:growth & development*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000032:analysis*; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D013025:Glycine max / Q000254:growth & development*
chemical_list: D000468:Alkalies; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012492:Salts; D012987:Soil