title: Adenosine Triphosphate Regresses Endometrial Explants in a Rat Model of Endometriosis.
abstract: The aim of this study was to determine the effects of adenosine triphosphate (ATP) in a rat endometriosis model. After surgical induction of endometriosis, 3 rats were killed, and explants were measured in the remaining 19 rats, which were then randomly assigned to 4 groups. Group 1 (n = 4) received normal saline (2 mL/d intragastric [IG]), group 2 (n = 4) gestrinone (0.5 mg/kg/d IG), group 3 (n = 5) ATP (3.4 mg/kg/d IG), and group 4 (n = 6) ATP (1.0 mg/kg/d; intramuscularly), respectively. Four weeks after medication, they were euthanized to evaluate histological features of explants and eutopic uterine tissues. To test the effect of ATP on the growth of eutopic endometrium stromal cells, proliferation rates of hEM15A cells at 24, 48, and 72 hours after treatment with different concentrations of ATP and vehicle control were detected with the Cell Counting Kit-8 (CCK-8) method. There was a significant difference between pretreatment and posttreatment volumes within group 2 (positive control; P = .048) and group 4 (P = .044). On condition that pretreatment implant size was similar in both groups (P = .516), regression of explants in group 4 was significantly higher than that in group 1 (negative control; P = .035). Epithelial cells were significantly better preserved in group 1 than in group 3 (P = .008) and group 4 (P = .037). The CCK-8 assay showed no significant difference in proliferation among hEM15A cells treated with ATP and controls. These results suggest that ATP regresses endometriotic tissues in a rat endometriosis model but has no impact on the growth of eutopic endometrium stromal cells.
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Yi
authors.affiliation: Reproductive Medicine Center, Peking University People's Hospital, Beijing, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Han
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ye
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Ruiqiong
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Pathology, Peking University People's Hospital, Beijing, China.
authors.forename: Kunkun
authors.initials: K
authors.lastname: Sun
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Cui
authors.affiliation: Gynecological Oncology Center, Peking University People's Hospital, Beijing, China changxiaohong@pkuph.edu.cn.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chang
mesh_terms: D000255:Adenosine Triphosphate / Q000008:administration & dosage*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D004195:Disease Models, Animal; D004715:Endometriosis / Q000473:pathology* / Q000517:prevention & control; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D000255:Adenosine Triphosphate
keywords: adenosine triphosphate (ATP); endometriosis; rat model