title: Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
abstract: Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). Their immunoregulation activities were further compared in vitro. The results showed that they displayed different structural features and immuno-enhancement activities. They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes. Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3(+)CD56(+) cells to some extent. These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
authors.affiliation: Laboratory Animal Center, Zhengzhou University, Zhengzhou, Henan Province 450052, PR China; The Third Affiliated Hospital of Zhengzhou University (Maternity and Child Care Service Hospital of Henan Province), Zhengzhou, Henan 450052, PR China. Electronic address: wangjunmin@zzu.edu.cn.
authors.forename: Junmin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory Animal Center, Zhengzhou University, Zhengzhou, Henan Province 450052, PR China.
authors.forename: Beilei
authors.initials: B
authors.lastname: Ge
authors.affiliation: Biotechnology Developing Center of Henan Academy of Sciences, Zhengzhou, Henan 450002, PR China.
authors.forename: Zihong
authors.initials: Z
authors.lastname: Li
authors.affiliation: The Third Affiliated Hospital of Zhengzhou University (Maternity and Child Care Service Hospital of Henan Province), Zhengzhou, Henan 450052, PR China; The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, PR China; School of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Fangxia
authors.initials: F
authors.lastname: Guan
authors.affiliation: Biotechnology Developing Center of Henan Academy of Sciences, Zhengzhou, Henan 450002, PR China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Li
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D018417:Th1 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D009005:Monosaccharides; D011134:Polysaccharides
keywords: Angelica sinensis polysaccharides; Immunoregulation; Structural analysis