title: Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity.
abstract: In the absence of the natural acceptor inorganic phosphate wild-type sucrose phosphorylase from Bifidobacterium adolescentis (BaSP) produces maltose (4-O-α-d-glucopyranosyl-d-glucose) and kojibiose (2-O-α-d-glucopyranosyl-d-glucose) as sole transfer products. A Q345F exchange switches the enzyme's regioselectivity from 2 to 3 exclusively, yielding the rare sugar nigerose (3-O-α-d-glucopyranosyl-d-glucose, sakebiose).
authors.affiliation: Institute for Organic Chemistry, Julius-Maximilians University Würzburg, Am Hubland C1, Würzburg, Germany. seibel@chemie.uni-wuerzburg.de.
authors.forename: M
authors.initials: M
authors.lastname: Kraus
authors.affiliation: Institute for Organic Chemistry, Julius-Maximilians University Würzburg, Am Hubland C1, Würzburg, Germany. seibel@chemie.uni-wuerzburg.de.
authors.forename: J
authors.initials: J
authors.lastname: Görl
authors.affiliation: Institute for Organic Chemistry, Julius-Maximilians University Würzburg, Am Hubland C1, Würzburg, Germany. seibel@chemie.uni-wuerzburg.de.
authors.forename: M
authors.initials: M
authors.lastname: Timm
authors.affiliation: Institute for Organic Chemistry, Julius-Maximilians University Würzburg, Am Hubland C1, Würzburg, Germany. seibel@chemie.uni-wuerzburg.de.
authors.forename: J
authors.initials: J
authors.lastname: Seibel
mesh_terms: D018360:Crystallography, X-Ray; D004187:Disaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D009154:Mutation; D006005:Phosphorylases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D004187:Disaccharides; C014637:laminaribiose; C008237:kojibiose; D006005:Phosphorylases