title: A novel role for Crp in controlling magnetosome biosynthesis in Magnetospirillum gryphiswaldense MSR-1.
abstract: Magnetotactic bacteria (MTB) are specialized microorganisms that synthesize intracellular magnetite particles called magnetosomes. Although many studies have focused on the mechanism of magnetosome synthesis, it remains unclear how these structures are formed. Recent reports have suggested that magnetosome formation is energy dependent. To investigate the relationship between magnetosome formation and energy metabolism, a global regulator, named Crp, which mainly controls energy and carbon metabolism in most microorganisms, was genetically disrupted in Magnetospirillum gryphiswaldense MSR-1. Compared with the wild-type or complemented strains, the growth, ferromagnetism and intracellular iron content of crp-deficient mutant cells were dramatically decreased. Transmission electron microscopy (TEM) showed that magnetosome synthesis was strongly impaired by the disruption of crp. Further gene expression profile analysis showed that the disruption of crp not only influenced genes related to energy and carbon metabolism, but a series of crucial magnetosome island (MAI) genes were also down regulated. These results indicate that Crp is essential for magnetosome formation in MSR-1. This is the first time to demonstrate that Crp plays an important role in controlling magnetosome biomineralization and provides reliable expression profile data that elucidate the mechanism of Crp regulation of magnetosome formation in MSR-1.
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.|France-China Bio-Mineralization and Nano-Structures Laboratory, Beijing 100193, P. R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.|France-China Bio-Mineralization and Nano-Structures Laboratory, Beijing 100193, P. R. China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.|France-China Bio-Mineralization and Nano-Structures Laboratory, Beijing 100193, P. R. China.
authors.forename: Jiesheng
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.|France-China Bio-Mineralization and Nano-Structures Laboratory, Beijing 100193, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.|France-China Bio-Mineralization and Nano-Structures Laboratory, Beijing 100193, P. R. China.
authors.forename: Jilun
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.|France-China Bio-Mineralization and Nano-Structures Laboratory, Beijing 100193, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods; D004734:Energy Metabolism; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D007501:Iron / Q000378:metabolism; D055944:Magnetosomes / Q000378:metabolism*; D042201:Magnetospirillum / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D007501:Iron