title: Rice OsVAMP714, a membrane-trafficking protein localized to the chloroplast and vacuolar membrane, is involved in resistance to rice blast disease.
abstract: Membrane trafficking plays pivotal roles in many cellular processes including plant immunity. Here, we report the characterization of OsVAMP714, an intracellular SNARE protein, focusing on its role in resistance to rice blast disease caused by the fungal pathogen Magnaporthe oryzae. Disease resistance tests using OsVAMP714 knockdown and overexpressing rice plants demonstrated the involvement of OsVAMP714 in blast resistance. The overexpression of OsVAMP7111, whose product is highly homologous to OsVAMP714, did not enhance blast resistance to rice, implying a potential specificity of OsVAMP714 to blast resistance. OsVAMP714 was localized to the chloroplast in mesophyll cells and to the cellular periphery in epidermal cells of transgenic rice plant leaves. We showed that chloroplast localization is critical for the normal OsVAMP714 functioning in blast resistance by analyzing the rice plants overexpressing OsVAMP714 mutants whose products did not localize in the chloroplast. We also found that OsVAMP714 was located in the vacuolar membrane surrounding the invasive hyphae of M. oryzae. Furthermore, we showed that OsVAMP714 overexpression promotes leaf sheath elongation and that the first 19 amino acids, which are highly conserved between animal and plant VAMP7 proteins, are crucial for normal rice plant growths. Our studies imply that the OsVAMP714-mediated trafficking pathway plays an important role in rice blast resistance as well as in the vegetative growth of rice.
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Sugano
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Nagao
authors.initials: N
authors.lastname: Hayashi
authors.affiliation: Functional Plant Research Unit, Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawagoe
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.|Graduate School and Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Mochizuki
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Inoue
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nishizawa
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
authors.forename: Chang-Jie
authors.initials: CJ
authors.lastname: Jiang
authors.affiliation: Center for Sustainable Resource Science, RIKEN, 1-7-22, Suehirocho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Minami
authors.initials: M
authors.lastname: Matsui
authors.affiliation: Disease Resistant Crops Research Unit, GMO Center, National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan. takatsuh@affrc.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takatsuji
mesh_terms: D002462:Cell Membrane; D002736:Chloroplasts / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D020022:Genetic Predisposition to Disease; D012275:Oryza / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology*; D050683:R-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology
chemical_list: D010940:Plant Proteins; D050683:R-SNARE Proteins; D020156:Salicylic Acid
keywords: Blast resistance; Chloroplast; Rice; VAMP; Vacuolar membrane