title: Identification of methylglyoxal as a major mutagen in wood and bamboo pyroligneous acids.
abstract: To identify the major mutagen in pyroligneous acid (PA), 10 wood and 10 bamboo pyroligneous acids were examined using the Ames test in Salmonella typhimurium strains TA100 and TA98. Subsequently, the mutagenic dicarbonyl compounds (DCs), glyoxal, methylglyoxal (MG), and diacetyl in PA were quantified using high-performance liquid chromatography, and the mutagenic contribution ratios for each DC were calculated relative to the mutagenicity of PA. Eighteen samples were positive for mutagens and showed the strongest mutagenicity in TA100 in the absence of S9 mix. MG had the highest mutagenic contribution ratio, and its presence was strongly correlated with the specific mutagenicity of PA. These data indicate that MG is the major mutagen in PA.
authors.affiliation: a Food Department , Nagoya City Public Health Research Institute , Nagoya , Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Onoda
authors.affiliation: a Food Department , Nagoya City Public Health Research Institute , Nagoya , Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Asanoma
authors.affiliation: b Graduate Division of Nutritional and Environmental Sciences , University of Shizuoka , Shizuoka , Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Nukaya
mesh_terms: D000818:Animals; D031723:Bambusa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003931:Diacetyl / Q000302:isolation & purification* / Q000633:toxicity; D006037:Glyoxal / Q000302:isolation & purification* / Q000633:toxicity; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000302:isolation & purification* / Q000633:toxicity; D011765:Pyruvaldehyde / Q000302:isolation & purification* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification; D014934:Wood / Q000737:chemistry*
chemical_list: D009153:Mutagens; D013729:Terpenes; C000600621:pyroligneous acid; D006037:Glyoxal; D011765:Pyruvaldehyde; D003931:Diacetyl
keywords: Ames test; dicarbonyl compound; methylglyoxal; mutagen; pyroligneous acid