title: Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans.
abstract: Mannose-6-phosphate (M-6-P) glycan analysis is important for quality control of therapeutic enzymes for lysosomal storage diseases. Here, we found that the analysis of glycans containing two M-6-Ps was highly affected by the hydrophilicity of the elution solvent used in high-performance liquid chromatography (HPLC). In addition, the performances of three fluorescent tags--2-aminobenzoic acid (2-AA), 2-aminobenzamide (2-AB), and 3-(acetyl-amino)-6-aminoacridine (AA-Ac)--were compared with each other for M-6-P glycan analysis using HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The best performance for analyzing M-6-P glycans was shown by 2-AA labeling in both analyses.
authors.affiliation: Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Kang
authors.affiliation: Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea; Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, Republic of Korea.
authors.forename: Ohsuk
authors.initials: O
authors.lastname: Kwon
authors.affiliation: Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Gil
authors.affiliation: Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea; Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, Republic of Korea. Electronic address: dboh@kribb.re.kr.
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
mesh_terms: D000585:Aminacrine / Q000031:analogs & derivatives; D062365:Aminobenzoates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008360:Mannosephosphates / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D062367:ortho-Aminobenzoates / Q000737:chemistry
chemical_list: D062365:Aminobenzoates; D005456:Fluorescent Dyes; D008360:Mannosephosphates; D011134:Polysaccharides; D062367:ortho-Aminobenzoates; C027693:mannose-6-phosphate; D000585:Aminacrine; C000219:anthranilamide
keywords: Fluorescent labeling tag; Glycan analysis; High-performance liquid chromatography (HPLC); Mannose-6-phosphate (M-6-P); Matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) mass spectrometry