title: Polysaccharide from Angelica sinensis protects chondrocytes from H2O2-induced apoptosis through its antioxidant effects in vitro.
abstract: This study aimed to explore the protective effects of Angelica sinensis polysaccharide (ASP) on rat chondrocyte injury induced by hydrogen peroxide (H2O2). Rat chondrocytes were cultured and treated with different concentrations of ASP alone or in combination with H2O2, and they were measured with cell viability, apoptosis, release of inflammatory cytokines, such as interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α), activity of superoxide dismutase (SOD), and catalase (CAT), and levels of malondialdehyde (MDA) production, respectively. In addition, quantitative real-time reverse transcription polymerase chain reaction was used to estimate the relative expression levels of osteoarthritis (OA)-associated genes, such as collagen type II (Col2a1), aggrecan, SOX9, matrix metalloproteinase (MMP)-1, -3, and -9, as well as tissue inhibitor of matrix metalloproteinase (TIMP)-1, respectively. Results indicated that ASP protected chondrocytes from H2O2-induced oxidative stress and subsequent cell injury through its antioxidant, antiapoptotic and anti-inflammatory effects in vitro. Our study suggests that ASP could become a therapeutic supplementation for the treatment of OA.
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Changzhou, Nanjing Medical University, Changzhou 213003, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhuang
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Changzhou, Nanjing Medical University, Changzhou 213003, China.
authors.forename: Nan-Wei
authors.initials: NW
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Changzhou, Nanjing Medical University, Changzhou 213003, China.
authors.forename: Gong-Ming
authors.initials: GM
authors.lastname: Gao
authors.affiliation: Laboratory of Clinical Orthopedics, The Second People's Hospital of Changzhou, Nanjing Medical University, Changzhou 213003, China.
authors.forename: Su
authors.initials: S
authors.lastname: Ni
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Changzhou, Nanjing Medical University, Changzhou 213003, China.
authors.forename: Kai-Song
authors.initials: KS
authors.lastname: Miao
authors.affiliation: Laboratory of Clinical Orthopedics, The Second People's Hospital of Changzhou, Nanjing Medical University, Changzhou 213003, China.
authors.forename: Chen-Kai
authors.initials: CK
authors.lastname: Li
authors.affiliation: Department of Orthopedics and Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. Electronic address: limingwang99@hotmail.com.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Wang
authors.affiliation: Division of Clinical Pharmacology, General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China; Department of Pharmacology, Nanjing Medical University School of Pharmacy, Nanjing 210029, China. Electronic address: hongg.xie@gmail.com.
authors.forename: Hong-Guang
authors.initials: HG
authors.lastname: Xie
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D019610:Cytoprotection / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D011134:Polysaccharides; D006861:Hydrogen Peroxide
keywords: Angelica sinensis polysaccharide; Antioxidant; Chondrocytes; Hydrogen peroxide; Osteoarthritis; Rat