title: Experimental infection with Brazilian Newcastle disease virus strain in pigeons and chickens.
abstract: This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after infection with a highly pathogenic Newcastle disease virus strain (São Joao do Meriti) and that, in chickens, prevent clinical signs. Infected pigeons shed the pathogenic strain, which was not detected in sentinel chickens or control birds. When the presence of Newcastle disease virus was analyzed in tissue samples by RT-PCR, in both species, the virus was most frequently found in the spleen. The vaccination regimen can prevent clinical disease in chickens and reduce viral shedding by chickens or pigeons. Biosecurity measures associated with vaccination programs are crucial to maintain a virulent Newcastle disease virus-free status in industrial poultry in Brazil.
authors.affiliation: Departamento de Medicina Veterinária, Universidade Estadual do Centro-Oeste (UNICENTRO), Guarapuava, PR, Brazil. Electronic address: adriano.carrasco@gmail.com.
authors.forename: Adriano de Oliveira Torres
authors.initials: Ade O
authors.lastname: Carrasco
authors.affiliation: Departamento de Medicina Veterinária, Universidade Estadual do Centro-Oeste (UNICENTRO), Guarapuava, PR, Brazil.
authors.forename: Meire Christina
authors.initials: MC
authors.lastname: Seki
authors.affiliation: Departamento de Medicina Veterinária, Universidade Estadual do Centro-Oeste (UNICENTRO), Guarapuava, PR, Brazil.
authors.forename: Jyan Lucas
authors.initials: JL
authors.lastname: Benevenute
authors.affiliation: Departamento de Medicina Veterinária, Universidade Estadual do Centro-Oeste (UNICENTRO), Guarapuava, PR, Brazil.
authors.forename: Priscila
authors.initials: P
authors.lastname: Ikeda
authors.affiliation: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil.
authors.forename: Aramis Augusto
authors.initials: AA
authors.lastname: Pinto
mesh_terms: D000825:Animal Structures / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001938:Brazil; D002645:Chickens; D010856:Columbidae; D004195:Disease Models, Animal; D018562:Disease Transmission, Infectious; D006385:Hemagglutination Inhibition Tests; D054884:Host-Pathogen Interactions; D009521:Newcastle Disease / Q000276:immunology / Q000473:pathology* / Q000635:transmission / Q000821:virology*; D009522:Newcastle disease virus / Q000254:growth & development* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral
keywords: Columba livia; Experimental infection; Gallus gallus; RT-PCR; Serology