title: HMGB1 silence could promote MCF-7 cell apoptosis and inhibit invasion and metastasis.
abstract: High mobility group box 1 (HMGB1), a non-histone nuclear protein, was associated with a variety of biological important processes, such as transcription, differentiation, extracellular signaling. As a cytokine or inflammatory mediator, more and more data showed that HMGB1 was involved in inflammatory diseases, cancers or autoimmune disease. However, few data focused on nucleic or cytoplasmic function of HMGB1. Therefore, the present study focused on cancer cells biological characteristics following HMGB1 silence. HMGB1 siRNAs were designed and chemically synthesized, and then transfected into the breast cancer cell line MCF-7 with lipofectamine 2000. The transcription and translation level of HMGB1 expression, proliferation, apoptosis, migration of MCF-7 were determined. The results demonstrated that HMGB1 silence inhibit invasion and migration and promote apoptosis of human breast cells; which indicated that HMGB1 silence might be a potential therapy targets.
authors.affiliation: Department of Immunology, Jiangsu University Zhenjiang 212013, Jiangsu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ni
authors.affiliation: Department of Immunology, Jiangsu University Zhenjiang 212013, Jiangsu, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Affiliated Fourth People's Hospital, Jiangsu University Zhenjiang 212001, China.
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Affiliated Fourth People's Hospital, Jiangsu University Zhenjiang 212001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Immunology, Jiangsu University Zhenjiang 212013, Jiangsu, China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Immunology, Jiangsu University Zhenjiang 212013, Jiangsu, China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Lu
authors.affiliation: The Affiliated Fourth People's Hospital, Jiangsu University Zhenjiang 212001, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Shen
authors.affiliation: The Affiliated Fourth People's Hospital, Jiangsu University Zhenjiang 212001, China.
authors.forename: Wenlin
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Immunology, Jiangsu University Zhenjiang 212013, Jiangsu, China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Immunology, Jiangsu University Zhenjiang 212013, Jiangsu, China.
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D024243:HMGB1 Protein / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D034741:RNA, Small Interfering
keywords: HMGB1; apoptosis; migration; proliferation