title: SMART Digest™ compared with pellet digestion for analysis of human immunoglobulin G1 in rat serum by liquid chromatography tandem mass spectrometry.
abstract: The newly developed SMART Digest™ kit was applied for the sample preparation of human immunoglobulin G1 (hIgG1) in rat serum prior to qualitative and quantitative analyses by liquid chromatography tandem mass spectrometry (LC-MS/MS). The sequence coverages obtained for the light and heavy chains of hIgG1A were 50 and 76%, respectively. The calibration curve was linear from 1.00 to 1000 μg/ml for three of four generic peptides. Overall, the SMART Digest™ kit resulted in similar quantitative data (linearity, sensitivity, accuracy, and precision) compared with the pellet digestion protocol. However, the SMART Digest™ required only 2 h of sample preparation with fewer reagents.
authors.affiliation: Drug Metabolism and Pharmacokinetics, Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland; Laboratoire de Spectrométrie de Masse BioOrganique, Institut Pluridisciplinaire Hubert Curien, CNRS-Université de Strasbourg, UMR 7178, 67087 Strasbourg, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Lanshoeft
authors.affiliation: Drug Metabolism and Pharmacokinetics, Novartis Institutes for Biomedical Research, 4056 Basel, Switzerland. Electronic address: olivier.heudi@novartis.com.
authors.forename: Olivier
authors.initials: O
authors.lastname: Heudi
authors.affiliation: Laboratoire de Spectrométrie de Masse BioOrganique, Institut Pluridisciplinaire Hubert Curien, CNRS-Université de Strasbourg, UMR 7178, 67087 Strasbourg, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Cianférani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood*; D057230:Limit of Detection; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D007074:Immunoglobulin G
keywords: Generic peptide; Human immunoglobulin G1; Liquid chromatography tandem mass spectrometry; Pellet digestion; SMART Digest™