title: β-lactoglobulin-pectin Nanoparticle-based Oral Drug Delivery System for Potential Treatment of Colon Cancer.
abstract: Colon cancer is one of the most common internal malignancies, and conventional chemotherapy is not effective in its treatment. Nanoparticles hold tremendous potential as an effective drug delivery system. The physicochemical properties of β-lactoglobulin, the main whey protein of cow's milk, such as its stability at low pH, its resistance to gastric protease, and its ability to bind hydrophobic ligands, give it potential for transporting drugs specifically for colon cancer. In the present research, β-lactoglobulin-pectin nanoparticles were designed to transfer a newly synthesized, anticancer platinum complex (bipyridine ethyl dithiocarbamate Pt(II) nitrate), to the colon. The effects of multiple factors on the size and the colloidal stability of the nanoparticles were studied using dynamic light scattering and scanning electron microscopy techniques. Results showed that the best particle size and highest colloidal stability were obtained in phosphate buffer, pH 4.5, with 0.5 mg/mL β-lactoglobulin and 0.025-0.05wt% pectin. The drug release profile in simulated gastrointestinal conditions demonstrated that β-lactoglobulin with a secondary coating is stable in acidic conditions but is able to release its cargo at pH 7. Hence, these nanoparticles have potential to serve as novel and effective vehicles for oral drug delivery preparations.
authors.affiliation: Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, P.O. Box 15815/3587, Tehran, Iran.
authors.forename: Zhila
authors.initials: Z
authors.lastname: Izadi
authors.affiliation: Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, P.O. Box 15815/3587, Tehran, Iran.
authors.forename: Adeleh
authors.initials: A
authors.lastname: Divsalar
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, P.O.Box 13145-1384, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
authors.affiliation: School of Biological Sciences, The University of Edinburgh, Roger Land Building, Alexander Crum Brown Road, Edinburgh EH9 3FF, UK.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Sawyer
mesh_terms: D000284:Administration, Oral; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry*; D009994:Osmolar Concentration; D010316:Particle Size; D010368:Pectins / Q000737:chemistry*; D010984:Platinum / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D007782:Lactoglobulins; D010984:Platinum; D010368:Pectins
keywords: Pt(II) complex; dynamic light scattering; scanning electron microscopy; β-lactoglobulin; β-lactoglobulin-pectin nanoparticles