title: Pathogens and fecal indicators in waste stabilization pond systems with direct reuse for irrigation: Fate and transport in water, soil and crops.
abstract: Wastewater use for irrigation is expanding globally, and information about the fate and transport of pathogens in wastewater systems is needed to complete microbial risk assessments and develop policies to protect public health. The lack of maintenance for wastewater treatment facilities in low-income areas and developing countries results in sludge accumulation and compromised performance over time, creating uncertainty about the contamination of soil and crops. The fate and transport of pathogens and fecal indicators was evaluated in waste stabilization ponds with direct reuse for irrigation, using two systems in Bolivia as case studies. Results were compared with models from the literature that have been recommended for design. The removal of Escherichia coli in both systems was adequately predicted by a previously-published dispersed flow model, despite more than 10years of sludge accumulation. However, a design equation for helminth egg removal overestimated the observed removal, suggesting that this equation may not be appropriate for systems with accumulated sludge. To assess the contamination of soil and crops, ratios were calculated of the pathogen and fecal indicator concentrations in soil or on crops to their respective concentrations in irrigation water (termed soil-water and crop-water ratios). Ratios were similar within each group of microorganisms but differed between microorganism groups, and were generally below 0.1mLg(-1) for coliphage, between 1 and 100mLg(-1) for Giardia and Cryptosporidium, and between 100 and 1000mLg(-1) for helminth eggs. This information can be used for microbial risk assessments to develop safe water reuse policies in support of the United Nations' 2030 Sustainable Development Agenda.
authors.affiliation: Department of Civil and Environmental Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, FL, United States. Electronic address: verbylam@mail.usf.edu.
authors.forename: M E
authors.initials: ME
authors.lastname: Verbyla
authors.affiliation: Centro de Aguas y Saneamiento Ambiental, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: M M
authors.initials: MM
authors.lastname: Iriarte
authors.affiliation: Centro de Aguas y Saneamiento Ambiental, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: A
authors.initials: A
authors.lastname: Mercado Guzmán
authors.affiliation: Centro de Aguas y Saneamiento Ambiental, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: O
authors.initials: O
authors.lastname: Coronado
authors.affiliation: Centro de Aguas y Saneamiento Ambiental, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: M
authors.initials: M
authors.lastname: Almanza
authors.affiliation: Department of Civil and Environmental Engineering, University of South Florida, 4202 E. Fowler Avenue, Tampa, FL, United States.
authors.forename: J R
authors.initials: JR
authors.lastname: Mihelcic
mesh_terms: D057914:Agricultural Irrigation; D001838:Bolivia; D003247:Conservation of Natural Resources; D018556:Crops, Agricultural; D004784:Environmental Monitoring; D061005:Ponds; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D062065:Wastewater
keywords: Microorganisms; Parasites; Reclaimed water; Sanitation; WHO Guidelines; Wastewater use