title: Effect of Resorbable Collagen Plug on Bone Regeneration in Rat Critical-Size Defect Model.
abstract: OBJECTIVE
  The purpose of this investigation was to examine the effect of resorbable collagen plug (RCP) on bone regeneration in rat calvarial critical-size defects.
  
  
  METHODS
  About 5-mm-diameter calvarial defects were created in forty 12-week-old male Sprague-Dawley rats and implanted with or without RCP. Animals were killed at 1, 2, 4, and 8 weeks postoperatively. After being killed, specimens were collected and subjected to micro-computed tomography (μCT) and histological analysis.
  
  
  RESULTS
  The μCT showed a significant increase of newly formed bone volume/tissue volume in RCP-implanted defect compared with controls at all designated time points. After 8 weeks, the defects implanted with RCP displayed almost complete closure. Hematoxylin and eosin staining of the decalcified sections confirmed these observations and evidenced active bone regeneration in the RCP group. In addition, Masson's trichrome staining demonstrated that RCP implantation accelerated the process of collagen maturation.
  
  
  CONCLUSIONS
  The RCP enhances bone regeneration in rat critical-size cranial defects, which suggest it might be a desired material for bone defect repair.
authors.affiliation: *Graduate Student, State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China. †Lecturer, Dental Implant Center, West China Hospital of Stomatology, Sichuan University, Chengdu, China. ‡Assistant Professor, Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. §Professor, State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Dong
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Guo
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Shiwen
authors.initials: S
authors.lastname: Zhang
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhou
authors.forename: Dutmanee
authors.initials: D
authors.lastname: Seriwatanachai
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D003094:Collagen / Q000502:physiology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000254:growth & development / Q000293:injuries / Q000473:pathology
chemical_list: D003094:Collagen