title: Patterned Thermoresponsive Microgel Coatings for Noninvasive Processing of Adherent Cells.
abstract: Cultivation of adherently growing cells in artificial environments is of utmost importance in medicine and biotechnology to accomplish in vitro drug screening or to investigate disease mechanisms. Precise cell manipulation, like localized control over adhesion, is required to expand cells, to establish cell models for novel therapies and to perform noninvasive cell experiments. To this end, we developed a method of gentle, local lift-off of mammalian cells using polymer surfaces, which are reversibly and repeatedly switchable between a cell-attractive and a cell-repellent state. This property was introduced through micropatterned thermoresponsive polymer coatings formed from colloidal microgels. Patterning was obtained through automated nanodispensing or microcontact printing, making use of unspecific electrostatic interactions between microgels and substrates. This process is much more robust against ambient conditions than covalent coupling, thus lending itself to up-scaling. As an example, wound healing assays were accomplished at 37 °C with highly increased precision in microfluidic environments.
authors.affiliation: Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology , 14476 Potsdam, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Uhlig
authors.affiliation: GeSiM mbH , 01454 Grosserkmannsdorf, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Wegener
authors.affiliation: GeSiM mbH , 01454 Grosserkmannsdorf, Germany.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Chemistry Physical and Biophysical Chemistry (PC III), Bielefeld University , 33615 Bielefeld, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Zeiser
authors.affiliation: Department of Chemistry Physical and Biophysical Chemistry (PC III), Bielefeld University , 33615 Bielefeld, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Bookhold
authors.affiliation: Physical Chemistry II, Bayreuth University , 95440 Bayreuth, Germany.
authors.forename: Inna
authors.initials: I
authors.lastname: Dewald
authors.affiliation: Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology , 14476 Potsdam, Germany.
authors.forename: Neus
authors.initials: N
authors.lastname: Godino
authors.affiliation: Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology , 14476 Potsdam, Germany.
authors.forename: Magnus
authors.initials: M
authors.lastname: Jaeger
authors.affiliation: Department of Chemistry Physical and Biophysical Chemistry (PC III), Bielefeld University , 33615 Bielefeld, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hellweg
authors.affiliation: Institute of Physical Chemistry and Polymer Physics, Leibniz Institute for Polymer Research Dresden , 01069 Dresden, Germany.|Chair of Physical Chemistry of Polymeric Materials, Technical University Dresden , 01069 Dresden, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Fery
authors.affiliation: Branch Bioanalytics and Bioprocesses (IZI-BB), Fraunhofer Institute for Cell Therapy and Immunology , 14476 Potsdam, Germany.
authors.forename: Claus
authors.initials: C
authors.lastname: Duschl
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D044085:Microfluidics / Q000379:methods; D013696:Temperature
chemical_list: D020099:Coated Materials, Biocompatible; D020100:Hydrogels