title: Short and long time effects of low temperature Plasma Activated Media on 3D multicellular tumor spheroids.
abstract: This work investigates the regionalized antiproliferative effects of plasma-activated medium (PAM) on colon adenocarcinoma multicellular tumor spheroid (MCTS), a model that mimics 3D organization and regionalization of a microtumor region. PAM was generated by dielectric barrier plasma jet setup crossed by helium carrier gas. MCTS were transferred in PAM at various times after plasma exposure up to 48 hours and effect on MCTS growth and DNA damage were evaluated. We report the impact of plasma exposure duration and delay before transfer on MCTS growth and DNA damage. Local accumulation of DNA damage revealed by histone H2AX phosphorylation is observed on outermost layers and is dependent on plasma exposure. DNA damage is completely reverted by catalase addition indicating that H2O2 plays major role in observed genotoxic effect while growth inhibitory effect is maintained suggesting that it is due to others reactive species. SOD and D-mannitol scavengers also reduced DNA damage by 30% indicating that O(2)(-)* and OH* are involved in H2O2 formation. Finally, PAM is able to retain its cytotoxic and genotoxic activity upon storage at +4 °C or -80 °C. These results suggest that plasma activated media may be a promising new antitumor strategy for colorectal cancer tumors.
authors.affiliation: Université de Toulouse ; UPS, INP ; LAPLACE; 118 route de Narbonne, F-31062 Toulouse, France.|CNRS ; LAPLACE; F-31062 Toulouse, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Judée
authors.affiliation: Université de Toulouse; ITAV-USR3505, F-31106 Toulouse, France.|CNRS; ITAV-USR3505, F-31106 Toulouse, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Fongia
authors.affiliation: Université de Toulouse; ITAV-USR3505, F-31106 Toulouse, France.|CNRS; ITAV-USR3505, F-31106 Toulouse, France.|CHU de Toulouse; F-31059 Toulouse, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ducommun
authors.affiliation: Université de Toulouse ; UPS, INP ; LAPLACE; 118 route de Narbonne, F-31062 Toulouse, France.|CNRS ; LAPLACE; F-31062 Toulouse, France.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Yousfi
authors.affiliation: Université de Toulouse; ITAV-USR3505, F-31106 Toulouse, France.|CNRS; ITAV-USR3505, F-31106 Toulouse, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Lobjois
authors.affiliation: Université de Toulouse ; UPS, INP ; LAPLACE; 118 route de Narbonne, F-31062 Toulouse, France.|CNRS ; LAPLACE; F-31062 Toulouse, France.
authors.forename: Nofel
authors.initials: N
authors.lastname: Merbahi
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D017209:Apoptosis / Q000528:radiation effects; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000528:radiation effects*; D003110:Colonic Neoplasms / Q000473:pathology; D004249:DNA Damage / Q000528:radiation effects; D006801:Humans; D058626:Plasma Gases; D018874:Spheroids, Cellular / Q000528:radiation effects*; D014407:Tumor Cells, Cultured
chemical_list: D058626:Plasma Gases