title: Design and development of PCR-free highly sensitive electrochemical assay for detection of telomerase activity using Nano-based (liposomal) signal amplification platform.
abstract: Telomerase, which has been detected in almost all kinds of cancer tissues, is considered as an important tumor marker for early cancer diagnostics. In the present study, an electrochemical method based on liposomal signal amplification platform is proposed for simple, PCR-free, and highly sensitive detection of human telomerase activity, extracted from A549 cells. In this strategy, telomerase reaction products, which immobilized on streptavidin-coated microplate, hybridized with biotinylated capture probes. Then, dopamine-loaded biotinylated liposomes are attached through streptavidin to biotinylated capture probes. Finally, liposomes are ruptured by methanol and the released-dopamine is subsequently measured using differential pulse voltammetry technique by multi-walled carbon nanotubes modified glassy carbon electrode. Using this strategy, the telomerase activity extracted from 10 cultured cancer cells could be detected. Therefore, this approach affords high sensitivity for telomerase activity detection and it can be regarded as an alternative to telomeric repeat amplification protocol assay, having the advantages of simplicity and less assay time.
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Alizadeh-Ghodsi
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Zavari-Nematabad
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Hamishehkar
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Akbarzadeh
authors.affiliation: Electroanalytical Chemistry Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
authors.forename: Tohid
authors.initials: T
authors.lastname: Mahmoudi-Badiki
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Faraz
authors.initials: F
authors.lastname: Zarghami
authors.affiliation: Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Future Industries Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pourhassan Moghaddam
authors.affiliation: Electroanalytical Chemistry Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran. Electronic address: i-alipour@tabrizu.ac.ir.
authors.forename: Esmaeel
authors.initials: E
authors.lastname: Alipour
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: zarghami@tbzmed.ac.ir.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
mesh_terms: D014408:Biomarkers, Tumor / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics; D019098:Telomerase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D014408:Biomarkers, Tumor; D008081:Liposomes; D037742:Nanotubes, Carbon; D019098:Telomerase
keywords: Carbon nanotubes; Differential pulse voltammetry; Dopamine; Liposome; Signal amplification; Telomerase activity