title: Cytotoxic cochlioquinone derivatives from the endophytic fungus Bipolaris sorokiniana derived from Pogostemon cablin.
abstract: Chemical investigation of the liquid culture of the endophytic fungus Bipolaris sorokiniana A606, which was isolated from the medicinal plant Pogostemon cablin resulted in the isolation of four new cytotoxic compounds, named isocochlioquinones D-E (1-2) and cochlioquinones G-H (3-4), along with five known cochlioquinone analogues (5-9). Their structures were determined on the basis of extensive spectroscopic analysis. Isocochlioquinone D (1) possessed a rare benzothiazin-3-one moiety and cochlioquinone G (3) was the first example of cochlioquinones bearing an indole-4,7-dione fragment. All of the isolates (1-9) were evaluated for their cytotoxic activities against MCF-7, NCI-H460, SF-268 and HepG-2 tumor cell lines by the sulforhodamine B (SRB) assay. Compounds 4 and 6-9, featuring a cochlioquinone core, exhibited potent cytotoxicities in vitro against the four tumor cell lines, and a preliminary structure-activity relationship of these compounds was also discussed.
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China; Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: wmzhang@gdim.cn.
authors.forename: Mo
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China.
authors.forename: Zhang-Hua
authors.initials: ZH
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China.
authors.forename: Yu-Chan
authors.initials: YC
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China.
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China.
authors.forename: Hao-Hua
authors.initials: HH
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China.
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China.
authors.forename: Sai-Ni
authors.initials: SN
authors.lastname: Li
authors.affiliation: Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Xianlie Road 100, Guangzhou 510070, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification; D001203:Ascomycota / Q000737:chemistry*; D016227:Benzoquinones / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor / Q000187:drug effects; D006801:Humans; D045563:Indolequinones / Q000737:chemistry* / Q000302:isolation & purification; D019686:Lamiaceae / Q000382:microbiology*; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D016227:Benzoquinones; D045563:Indolequinones; C024732:indole-4,7-dione
keywords: Bipolaris sorokiniana; Cochlioquinones; Cytotoxic activity; Endophytic fungus; Pogostemon cablin