title: Contribution of carboxyl modified chiral mesoporous silica nanoparticles in delivering doxorubicin hydrochloride in vitro: pH-response controlled release, enhanced drug cellular uptake and cytotoxicity.
abstract: In this study, dual functionalized mesoporous silica nanoparticle (Dual-MSN) with functions of carboxyl modification and chirality was successfully developed and its special contribution in delivering doxorubicin hydrochloride (DOX) in vitro was mainly studied. Characteristics of Dual-MSN and its application as DOX carrier were intensively explored by comparing with naked non-functionalized MSN (Naked MSN). The results indicated that both Naked MSN and Dual-MSN significantly controlled DOX release due to the release hindrance caused by mesopores. As expected, Dual-MSN exhibited obvious enhanced pH-response because of its negative charges of carboxyl groups. DOX loaded Naked MSN and DOX loaded Dual-MSN presented better cytotoxicity than DOX due to carrier-mediated endocytosis and the favorable intercalation of DOX into DNA in the nuclei. The cytotoxicity of DOX loaded Dual-MSN was better than DOX loaded Naked MSN owing to its enhanced cellular uptake induced by chirality of Dual-MSN, demonstrating that double functions of Dual-MSN had unique advantages in improving antitumor effect of DOX towards MCF-7 cells and thus confirming its special contribution in DOX delivery.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Life Science & Biopharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Du
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Life Science & Biopharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, China. Electronic address: jinghuaxu2002@tom.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China. Electronic address: li_sanming2013@163.com.
authors.forename: Sanming
authors.initials: S
authors.lastname: Li
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004317:Doxorubicin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D012822:Silicon Dioxide; D004317:Doxorubicin
keywords: Carboxyl modification; Chiral mesoporous silica nanoparticles; Doxorubicin hydrochloride; pH-response