title: Induction of nodD Gene in a Betarhizobium Isolate, Cupriavidus sp. of Mimosa pudica, by Root Nodule Phenolic Acids.
abstract: A range of phenolic acids, viz., p-coumaric acid, 4-hydroxybenzaldehyde, 4-hydroxybenzoic acid, protocatechuic acid, caffeic acid, ferulic acid, and cinnamic acid have been isolated and identified by LC-MS analysis in the roots and root nodules of Mimosa pudica. The effects of identified phenolic acids on the regulation of nodulation (nod) genes have been evaluated in a betarhizobium isolate of M. pudica root nodule. Protocatechuic acid and p-hydroxybenzoic acid were most effective in inducing nod gene, whereas caffeic acid had no significant effect. Phenylalanine ammonia lyase, peroxidase, and polyphenol oxidase activities were estimated, indicating regulation and metabolism of phenolic acids in root nodules. These results showed that nodD gene expression of betarhizobium is regulated by simple phenolic acids such as protocatechuic acid and p-hydroxybenzoic acid present in host root nodule and sustains nodule organogenesis.
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore, WB, 721102, India. mandalsm@gmail.com.
authors.forename: Santi M
authors.initials: SM
authors.lastname: Mandal
authors.affiliation: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, 304 022, India.
authors.forename: Dipjyoti
authors.initials: D
authors.lastname: Chakraborty
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Khargpur, Kharagpur, WB, 721302, India.
authors.forename: Suhrid R
authors.initials: SR
authors.lastname: Dutta
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Khargpur, Kharagpur, WB, 721302, India.
authors.forename: Ananta K
authors.initials: AK
authors.lastname: Ghosh
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore, WB, 721102, India.
authors.forename: Bikas R
authors.initials: BR
authors.lastname: Pati
authors.affiliation: Institute of Microbial Technology, Chandigarh, 160036, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Korpole
authors.affiliation: Amity University, Noida, 201313, India. drdebaratipaul@gmail.com.
authors.forename: Debarati
authors.initials: D
authors.lastname: Paul
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D049921:Cupriavidus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D062385:Hydroxybenzoates / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D031298:Mimosa / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D053204:Root Nodules, Plant / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D062385:Hydroxybenzoates; C060773:NodD protein, Bacteria; C017616:phenolic acid