title: Effects of Adding Vindoline and MeJA on Production of Vincristine and Vinblastine, and Transcription of their Biosynthetic Genes in the Cultured CMCs of Catharanthus roseus.
abstract: Vincristine and vinblastine were found by Liquid Chromatography-Mass Spectrometry (LC-MS) in Catharanthus roseuscambial meristem cells (CMCs) jointly treated with 0.25 mM vindoline and methyl jasmonate (MeJA), suggesting that C. roseus CMCs contain a complete set of the enzymes which are in response to convert vindoline into vincristine and vinblastine. Based on the facts that the transcript levels of vindoline-biosynthetic genes (STR, SGD and D4H) were up-regulated instead of being down-regulated by adding itself to the culture, and that the transcriptional factor ORCA3 was up-regulated simultaneously, we further confirmed that the transcription of STR, SGD, D4H was manipulated by ORCA3.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Zhang
authors.forename: Jiazeng
authors.initials: J
authors.lastname: Yang
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zi
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhu
authors.forename: Liyan
authors.initials: L
authors.lastname: Song
authors.forename: Rongmin
authors.initials: R
authors.lastname: Yu
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis; D058506:Cambium / Q000166:cytology* / Q000378:metabolism; D030008:Catharanthus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology*; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000494:pharmacology; D014750:Vincristine / Q000096:biosynthesis*
chemical_list: D000085:Acetates; D000972:Antineoplastic Agents, Phytogenic; D003517:Cyclopentanes; D054883:Oxylipins; C009667:vindoline; D014750:Vincristine; D014747:Vinblastine; C072239:methyl jasmonate