title: Transcriptome Analysis and Discovery of Genes Relevant to Development in Bradysia odoriphaga at Three Developmental Stages.
abstract: Bradysia odoriphaga (Diptera: Sciaridae) is the most important pest of Chinese chive (Allium tuberosum) in Asia; however, the molecular genetics are poorly understood. To explore the molecular biological mechanism of development, Illumina sequencing and de novo assembly were performed in the third-instar, fourth-instar, and pupal B. odoriphaga. The study resulted in 16.2 Gb of clean data and 47,578 unigenes (≥125 bp) contained in 7,632,430 contigs, 46.21% of which were annotated from non-redundant protein (NR), Gene Ontology (GO), Clusters of Orthologous Groups (COG), Eukaryotic Orthologous Groups (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. It was found that 19.67% of unigenes matched the homologous species mainly, including Aedes aegypti, Culex quinquefasciatus, Ceratitis capitata, and Anopheles gambiae. According to differentially expressed gene (DEG) analysis, 143, 490, and 309 DEGs were annotated as involved in the developmental process in the GO database respectively, in the comparisons of third-instar and fourth-instar larvae, third-instar larvae and pupae, and fourth-instar larvae and pupae. Twenty-five genes were closely related to these processes, including developmental process, reproduction process, and reproductive organs development and programmed cell death (PCD). The information of unigenes assembled in B. odoriphaga through transcriptome and DEG analyses could provide a detailed genetic basis and regulated information for elaborating the developmental mechanism from the larval, pre-pupal to pupal stages of B. odoriphaga.
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Gao
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Institute of Quality Standard & Testing Technology for Agri-products, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Xianhong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Zhuang
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, China.
authors.forename: Rumei
authors.initials: R
authors.lastname: Li
mesh_terms: D000818:Animals; D027782:Chive / Q000469:parasitology; D004175:Diptera / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D016678:Genome; D059014:High-Throughput Nucleotide Sequencing; D019476:Insect Proteins / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000254:growth & development; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000469:parasitology; D011679:Pupa / Q000235:genetics / Q000254:growth & development; D059467:Transcriptome
chemical_list: D019476:Insect Proteins