title: Discovery of active components in herbs using chromatographic separation coupled with online bioassay.
abstract: Discovery of bioactive compounds from complex mixtures is a challenge. In past decades, several strategies were developed and implemented for rapid and effective screening and characterization of bioactive components in complex matrices. This review mainly focused on the online strategies, which integrated the separation science, mass spectrometry, and bioactivity screening in a single platform, allowing simultaneous screening and characterization of active compounds from complex matrices, especially from the herbs. The online screening methodologies, including pre-column affinity-based screening and post-column bioassay, were discussed and their applied examples were also presented to illustrate the strengths and limitations of these approaches.
authors.affiliation: The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Li
authors.initials: L
authors.lastname: De-Qiang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: China Science and Technology Exchange Center, Beijing 100045, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address: lishaoping@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Shao-Ping
mesh_terms: D001681:Biological Assay; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D028321:Plant Preparations / Q000032:analysis / Q000737:chemistry; D014462:Ultrafiltration
chemical_list: D028321:Plant Preparations
keywords: Active components; Chromatographic separation; Complex matrices; Herbs; Online screening