title: Cyclophilin A is a new M cell marker of bovine intestinal epithelium.
abstract: Microfold (M) cells in the follicle-associated epithelium (FAE) of Peyer's patches contribute to the mucosal immune response by the transcytosis of microorganisms. The mechanism by which M cells take up microorganisms, and the functional proteins by which they do this, are not clear. In order to explore one such protein, we developed a 2H5-F3 monoclonal antibody (2H5-F3 mAb) through its binding to bovine M cells, and identified the antibody reactive molecule as cyclophilin A (Cyp-A). The localization patterns of Cyp-A were very similar to the localization pattern of cytokeratin (CK) 18-positive M cells. Cyp-A was identified at the luminal surface of CK18-positive M cells in bovine jejunal and ileal FAE. The membranous localization of Cyp-A in the bovine intestinal cell line (BIE cells) increased as cells differentiated toward M cells, as determined by flow cytometry analysis. Additionally, BIE cells released Cyp-A to the extracellular space and the differentiation of BIE cells to M cells increased the secretion of Cyp-A, as determined by western blotting. Accordingly, Cyp-A may be localized in M cells in the small intestinal epithelium of cattle. The rise of the membranous localization and secretion of Cyp-A by differentiation toward M cells indicates that Cyp-A has an important role in the function of M cells. While Cyp-A of the M cell membrane may contribute to the uptake of viruses with peptidyl-prolyl cis-trans isomerase activity, in the extracellular space Cyp-A may work as a chemokine and contribute to the distribution of immuno-competent cells.
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hondo
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Someya
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Nagasawa
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Terada
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.|Department of Physiology and Metabolism, Brain/Liver Interface Medicine Research Center, Kanazawa University, Kanazawa, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Xiangning
authors.initials: X
authors.lastname: Chen
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Shyuichi
authors.initials: S
authors.lastname: Ohwada
authors.affiliation: Food Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, UK.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Rose
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Nochi
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan. asosan@bios.tohoku.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D002853:Chromatography, Liquid; D003106:Colon / Q000166:cytology; D021984:Cyclophilin A / Q000378:metabolism*; D004386:Duodenum / Q000166:cytology; D007082:Ileum / Q000166:cytology; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism*; D007583:Jejunum / Q000166:cytology; D008297:Male; D008807:Mice, Inbred BALB C; D008871:Microvilli / Q000378:metabolism; D009305:Nasopharynx / Q000166:cytology; D010455:Peptides / Q000032:analysis; D010581:Peyer's Patches / Q000166:cytology / Q000648:ultrastructure; D053719:Tandem Mass Spectrometry
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D010455:Peptides; D021984:Cyclophilin A
keywords: Bovine intestinal epithelium; Cyclophilin A; Follicle associated epithelium; M cell; Peyer’s patch