title: Usefulness of oocyte accumulation in low ovarian response for PGS.
abstract: This is an observational study of the response to ovarian stimulation and preimplantational genetic screening (PGS) cycles of 188 patients with a foreseen high aneuploid rate, undergoing two or three stimulation cycles (2SC and 3SC) and oocyte vitrification to accumulate oocytes (Accumulation group = 112 patients) compared to patients undergoing one stimulation cycle (1SC Group= 76 patients) and fresh embryo transfer, between January 2011 and July 2014. Accumulation was performed when <10 MII oocytes were retrieved. Oocytes were vitrified for later warming and IVF, when the planned number of oocytes was achieved. After PGS, euploid embryos were transferred. Comparing 2SC Group with 3SC Group, AMH, AFC, number of oocytes retrieved per pick-up and total number of biopsied embryos were significantly higher in the 2SC Group. After chromosome analysis, 18.5% of biopsied embryos were euploid and 58.9% patients reached embryo transfer. There were no differences in pregnancy rates per patient between the 1SC, 2SC and 3SC Groups (36.8%, 34.9% and 31.0%, respectively) or per embryo transfer (59.6, 56.8 and 60%, respectively). In patients with <10 MII oocytes after ovarian stimulation undergoing PGS, accumulating oocytes can render a pregnancy rate per patient and per embryo transfer comparable to those of fresh PGS cycles.
authors.affiliation: a Servicio De Medicina De La Reproducción, Department of Obstetrics, Gynaecology and Reproduction, Hospital Universitario Quirón Dexeus , Barcelona , Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Martínez
authors.affiliation: a Servicio De Medicina De La Reproducción, Department of Obstetrics, Gynaecology and Reproduction, Hospital Universitario Quirón Dexeus , Barcelona , Spain.
authors.forename: Cayetana
authors.initials: C
authors.lastname: Barbed
authors.affiliation: a Servicio De Medicina De La Reproducción, Department of Obstetrics, Gynaecology and Reproduction, Hospital Universitario Quirón Dexeus , Barcelona , Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Parriego
authors.affiliation: a Servicio De Medicina De La Reproducción, Department of Obstetrics, Gynaecology and Reproduction, Hospital Universitario Quirón Dexeus , Barcelona , Spain.
authors.forename: Miquel
authors.initials: M
authors.lastname: Solé
authors.affiliation: a Servicio De Medicina De La Reproducción, Department of Obstetrics, Gynaecology and Reproduction, Hospital Universitario Quirón Dexeus , Barcelona , Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Rodríguez
authors.affiliation: a Servicio De Medicina De La Reproducción, Department of Obstetrics, Gynaecology and Reproduction, Hospital Universitario Quirón Dexeus , Barcelona , Spain.
authors.forename: Buenaventura
authors.initials: B
authors.lastname: Coroleu
mesh_terms: D000328:Adult; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D054315:Oocyte Retrieval / Q000379:methods*; D009865:Oocytes; D017063:Outcome Assessment, Health Care; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D058989:Vitrification
keywords: Low ovarian response; Preimplantation Genetic Screening; oocyte accumulation; oocyte vitrification; pregnancy rate