title: Cisplatin-Induced Non-Oliguric Acute Kidney Injury in a Pediatric Experimental Animal Model in Piglets.
abstract: OBJECTIVE
  To design an experimental pediatric animal model of acute kidney injury induced by cisplatin.
  
  
  METHODS
  Prospective comparative observational animal study in two different phases. Acute kidney injury was induced using three different doses of cisplatin (2, 3 and 5 mg/kg). The development of nephrotoxicity was assessed 2 to 4 days after cisplatin administration by estimating biochemical parameters, diuresis and renal morphology. Analytical values and renal morphology were compared between 15 piglets treated with cisplatin 3 mg/kg and 15 control piglets in the second phase of the study.
  
  
  RESULTS
  41 piglets were studied. The dose of 3 mg/kg administered 48 hours before the experience induced a significant increase in serum creatinine and urea without an increase in potassium levels. Piglets treated with cisplatin 3 mg/kg had significantly higher values of creatinine, urea, phosphate and amylase, less diuresis and lower values of potassium, sodium and bicarbonate than control piglets. Histological findings showed evidence of a dose-dependent increase in renal damage.
  
  
  CONCLUSIONS
  a dose of 3 mg/kg of cisplatin induces a significant alteration in renal function 48 hours after its administration, so it can be used as a pediatric animal model of non-oliguric acute kidney injury.
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Santiago
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Sarah Nicole
authors.initials: SN
authors.lastname: Fernández
authors.affiliation: Laboratory of Renal Physiopathology, Department of Nephrology, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Lázaro
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: González
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Urbano
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: López
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Solana
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Toledo
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Jimena
authors.initials: J
authors.lastname: Del Castillo
authors.affiliation: Complutense University of Madrid, Madrid, Spain.|Laboratory of Renal Physiopathology, Department of Nephrology, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Tejedor
authors.affiliation: Paediatric Intensive Care Department. Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Hospital General Universitario Gregorio Marañón, Madrid, Spain.|Spanish Health Institute Carlos III Maternal, Child Health and Development Network, Madrid, Spain.|Complutense University of Madrid, Madrid, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: López-Herce
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000628:therapy; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D004195:Disease Models, Animal; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011446:Prospective Studies; D017582:Renal Replacement Therapy; D034421:Sus scrofa
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin