title: Role of ADAMTS1 and ADAMTS5 in male infertility.
abstract: The aim of this study was to investigate the relationship of infertility with metalloproteinases ADAMTS1 and ADAMTS5, which are known to be responsible for the degradation of extracellular matrix (ECM) proteins associated with many diseases. ECM is the noncellular component that provides structural and biochemical support to the surrounding cells required for tissue morphogenesis, differentiation and homoeostasis. Sixty infertile individuals and 10 healthy semen donors were included in this study. The infertile individuals were classified as normozoospermia (NS; n = 20), oligozoospermia (OS; n = 20), azoospermia (AS; n = 20) groups. ADAMTS1 and ADAMTS5 protein levels in semen were analysed by Western blot. ADAMTS1 protein level was 3.0-, 3.3- and 1.6-fold lower in the OS, AS and NS groups, respectively, than in the control group (P < 0.001). ADAMTS5 protein level was 3.2-, 2.7- and 1.4-fold lower in the OS, AS and NS groups, respectively, than in the control group (P < 0.001). Sperm count and sperm motility showed a negative correlation with the levels of ADAMTS1 and ADAMTS5 protein expression: r = -0.477, r = -0.470; and r = -0.332, r = -0.275 respectively (P < 0.001). In conclusion, ADAMTS1 and ADAMTS5 protein expressions in semen are significantly related with sperm production. It is very important to understand molecular function and organisation of ADAMTSs which will be significant in enlightening the process of spermatogenesis in male infertility.
authors.affiliation: Department of Medical Biology, School of Medicine, Ankara University, Ankara, Turkey.
authors.forename: S E
authors.initials: SE
authors.lastname: Aydos
authors.affiliation: Department of Medical Biology, School of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Yukselten
authors.affiliation: Department of Medical Biology, School of Medicine, Ankara University, Ankara, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Sunguroglu
authors.affiliation: Department of Medical Biology, School of Medicine, Turgut Ozal University, Ankara, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Demircan
authors.affiliation: Department of Urology, School of Medicine, Ankara University, Ankara, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Aydos
mesh_terms: D000071097:ADAMTS1 Protein / Q000235:genetics / Q000378:metabolism*; D000071116:ADAMTS5 Protein / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D053713:Azoospermia / Q000235:genetics / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007248:Infertility, Male / Q000235:genetics / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009845:Oligospermia / Q000235:genetics / Q000378:metabolism; D012661:Semen / Q000378:metabolism*; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility; D013091:Spermatogenesis / Q000235:genetics*; D013739:Testosterone / Q000097:blood; D055815:Young Adult
chemical_list: D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human
keywords: Extracellular matrix; male infertility; metalloproteinases