title: Phenotypic and genotypic approach to characterize Arcanobacterium pluranimalium isolated from bovine milk samples.
abstract: In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Schubertstr. 81, 35392, Giessen, Germany.
authors.forename: Jörn-Peter
authors.initials: JP
authors.lastname: Wickhorst
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
authors.forename: Abdulwahed Ahmed
authors.initials: AA
authors.lastname: Hassan
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Schubertstr. 81, 35392, Giessen, Germany.
authors.forename: Osama
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Tiergesundheitsdienst Bayern e.V. Eutergesundheitsdienst, Senator-Gerauer-Straße 23, 85586, Grub, Germany.
authors.forename: Reglindis
authors.initials: R
authors.lastname: Huber-Schlenstedt
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, Schubertstr. 81, 35392, Giessen, Germany. christoph.laemmler@vetmed.uni-giessen.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, 35392, Giessen, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: Entwicklung Bioanalyse, Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359, Bremen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Timke
authors.affiliation: Institut für Lebensmittelqualität und-sicherheit, Stiftung Tierärztliche Hochschule Hannover, 30173, Hannover, Germany.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdulmawjood
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D055667:Arcanobacterium / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002417:Cattle; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S