title: Octylphenol induced gene expression in testes of Frog, Rana chensinensis.
abstract: Octylphenol (OP) is an endocrine-disrupting chemical (EDC), which can disrupt the reproductive system. To understand the effect of OP, a subtractive cDNA library was constructed using suppression subtractive hybridization (SSH) to identify alterations of gene transcription in the testes of the frog Rana chensinensis after OP exposure. Two hundred positive clones were selected and 134 sequences of gene fragments were produced from the subtractive library randomly. These genes were identified to be involved in metabolic process, cellular process, biological regulation, stimulus, immune system and female pregnancy process. In order to verify the efficiency of the subtractive cDNA library, PSG9 and PAPP-A were analyzed further as two representatives of differentially expressed transcription genes using semi-quantitative RT-PCR. Our result was the first successful construction of the subtractive cDNA library in frog testes after OP treatment. Based on this cDNA library, OP was shown to affect multiple physiological processes including inducing immune response, disrupting the steroid hormone synthesis and influencing spermatogenesis in the testis by up-regulation of specific genes.
authors.affiliation: Co-Innovation Center for Qinba Regions'Sustainable Development, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Li
authors.affiliation: Co-Innovation Center for Qinba Regions'Sustainable Development, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Co-Innovation Center for Qinba Regions'Sustainable Development, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, China. Electronic address: yu-huizhang@snnu.edu.cn.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D008297:Male; D009693:Nucleic Acid Hybridization; D010636:Phenols / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D011898:Ranidae / Q000235:genetics*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D010636:Phenols; C474055:octylphenol
keywords: Different gene expression; Gene transcription; Octylphenol; Rana chensinensis; Suppression Subtractive Hybridization (SSH); Testis