title: Comparative prognostic relevance of breast intra-tumoral microvessel density evaluated by CD105 and CD146: A pilot study of 42 cases.
abstract: Angiogenesis is a key process for metastatic progression. While it has been established that the evaluation of breast tumoral microvessel density by CD105 marker is a potential prognostic parameter, its evaluation by CD146 marker has been poorly studied.
  
  
  OBJECTIVE
  The purpose of this study was to compare the prognostic value of intra-tumoral microvessel density assayed by CD105 and CD146 in early breast cancer patients.
  
  
  METHODS
  42 women with breast infiltrative ductal carcinoma (I and II-stages) were retrospectively reviewed. Intra-tumoral microvessel density was immunohistochemically examined using antibodies anti-CD105 and CD146 in paraffin-embedded tissues, and their association with classical prognostic-markers, metastatic recurrence, metastasis-free survival and overall survival was analyzed.
  
  
  RESULTS
  High microvessel density assessed by CD146 was significantly associated with a higher risk of developing metastasis (p=0.0310) and a shorter metastasis-free survival (p=0.0197). In contrast, when we used the CD105-antibody, we did not find any significant association. Finally, CD146 showed to be an independent predictive indicator for metastasis-free survival (p=0.0055).
  
  
  CONCLUSIONS
  Our data suggest that the intra-tumoral microvessel density evaluated by CD146 may be a more suitable predictor of metastatic development than that evaluated by CD105 in early breast cancer.
authors.affiliation: Laboratorio de Inmunohematología, Instituto de Biología y Medicina Experimental (IBYME)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Leandro Marcelo
authors.initials: LM
authors.lastname: Martinez
authors.affiliation: Laboratorio de Inmunohematología, Instituto de Biología y Medicina Experimental (IBYME)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Vivian
authors.initials: V
authors.lastname: Labovsky
authors.affiliation: Departamento de Bioestadística, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: María de Luján
authors.initials: Mde L
authors.lastname: Calcagno
authors.affiliation: Departamento de Anatomía Patológica, Hospital Italiano, Juan Domingo Perón 4190, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Kevin Mauro
authors.initials: KM
authors.lastname: Davies
authors.affiliation: Departamento de Anatomía Patológica, Hospital Italiano, Juan Domingo Perón 4190, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Hernán Garcia
authors.initials: HG
authors.lastname: Rivello
authors.affiliation: Departamento de Anatomía Patológica, Hospital Italiano, Juan Domingo Perón 4190, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Wernicke
authors.affiliation: Laboratorio de Química de Proteoglicanos y Matriz Extracelular, Instituto de Biología y Medicina Experimental (IBYME)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Calvo
authors.affiliation: Laboratorio de Inmunohematología, Instituto de Biología y Medicina Experimental (IBYME)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: alejachase@gmail.com.
authors.forename: Norma Alejandra
authors.initials: NA
authors.lastname: Chasseing
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000098:blood supply* / Q000401:mortality / Q000473:pathology; D051929:CD146 Antigen / Q000032:analysis; D018270:Carcinoma, Ductal, Breast / Q000098:blood supply* / Q000401:mortality / Q000473:pathology; D000071063:Endoglin / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology; D010865:Pilot Projects; D011379:Prognosis; D016016:Proportional Hazards Models; D012189:Retrospective Studies
chemical_list: D014408:Biomarkers, Tumor; D051929:CD146 Antigen; C064206:ENG protein, human; D000071063:Endoglin; C497902:MCAM protein, human
keywords: Breast cancer; CD105; CD146; Intra-tumoral microvessel density; Prognostic significance