title: Acceleration of diabetic wound healing by a cryopreserved living dermal substitute created by micronized amnion seeded with fibroblasts.
abstract: Bioengineered dermal substitutes have been used for the treatment of diabetic ulcers in clinics and achieved satisfactory results. However, constructing traditional tissue engineered dermal substitutes with two-step method is high-cost, time-consuming and greatly decreases fibroblast proliferative activity because of repeated trypsinization. Inthisstudy, we created a 3D micronized amniotic membrane (mAM) and used it as a natural microcarrier for ex vivo culture and amplification of human dermal fibroblasts (HDF) combined with the rotary cell culture system (RCCS). This one-step mAM-RCCS method couldamplify HDF quickly and construct a dermal substitute HDF-mAM simultaneously. To facilitate the clinical application of mAM-RCCS, anoptimized storage method was used.Post-thawing HDF-mAM retained high cell viability, intact cell morphology and active peptide secretion. When transplanted to the wounds of db/db mice, cryopreserved HDF-mAM promoted vascularization and diabetic wound healing significantly. These results demonstrate the potential application of cryopreserved HDF-mAM as a living dermal substitutefor treating diabetic ulcers and other chronic wounds in clinics.
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Shizhao
authors.initials: S
authors.lastname: Ji
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wu
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Song
authors.initials: S
authors.lastname: Tian
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Xingtong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Luo
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: He
authors.initials: H
authors.lastname: Fang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Shichu
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Burns Center of Changhai Hospital, The Second Military Medical University Shanghai, China.
authors.forename: Zhaofan
authors.initials: Z
authors.lastname: Xia
keywords: Amniotic membrane; cryopreservation; dermal substitute; diabetes; fibroblasts; microcarrier; wound healing