title: Mass spectrometry imaging of rat brain lipid profile changes over time following traumatic brain injury.
abstract: Mild traumatic brain injury (TBI) is a common public health issue that may contribute to chronic degenerative disorders. Membrane lipids play a key role in tissue responses to injury, both as cell signals and as components of membrane structure and cell signaling. This study demonstrates the ability of high resolution mass spectrometry imaging (MSI) to assess sequences of responses of lipid species in a rat controlled cortical impact model for concussion.
  
  
  
  A matrix of implanted silver nanoparticles was implanted superficially in brain sections for matrix-assisted laser desorption (MALDI) imaging of 50μm diameter microdomains across unfixed cryostat sections of rat brain. Ion-mobility time-of-flight MS was used to analyze and map changes over time in brain lipid composition in a rats after Controlled Cortical Impact (CCI) TBI.
  
  
  
  Brain MS images showed changes in sphingolipids near the CCI site, including increased ceramides and decreased sphingomyelins, accompanied by changes in glycerophospholipids and cholesterol derivatives. The kinetics differed for each lipid class; for example ceramides increased as early as 1 day after the injury whereas other lipids changes occurred between 3 and 7 days post injury.
  
  
  
  Silver nanoparticles MALDI matrix is a sensitive new tool for revealing previously undetectable cellular injury response and remodeling in neural, glial and vascular structure of the brain.
  
  
  
  Lipid biochemical and structural changes after TBI could help highlighting molecules that can be used to determine the severity of such injuries as well as to evaluate the efficacy of potential treatments.
authors.affiliation: Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
authors.forename: Aurelie
authors.initials: A
authors.lastname: Roux
authors.affiliation: Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Muller
authors.affiliation: Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
authors.forename: Shelley N
authors.initials: SN
authors.lastname: Jackson
authors.affiliation: Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Post
authors.affiliation: Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States.
authors.forename: Katherine
authors.initials: K
authors.lastname: Baldwin
authors.affiliation: University Hospitals of Cleveland, Cleveland, OH 44106, United States.
authors.forename: Barry
authors.initials: B
authors.lastname: Hoffer
authors.affiliation: Departments of Otolaryngology, Neurobiology, Communication Sciences & Disorders, and Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, United States.
authors.forename: Carey D
authors.initials: CD
authors.lastname: Balaban
authors.affiliation: Ionwerks, Inc, Houston, TX 77002, United States.
authors.forename: Damon
authors.initials: D
authors.lastname: Barbacci
authors.affiliation: Ionwerks, Inc, Houston, TX 77002, United States.
authors.forename: J Albert
authors.initials: JA
authors.lastname: Schultz
authors.affiliation: Center for Neuroscience and Regenerative Medicine, Department of Pharmacology, Uniformed Services University, Bethesda, MD 20814, United States.
authors.forename: Shawn
authors.initials: S
authors.lastname: Gouty
authors.affiliation: Center for Neuroscience and Regenerative Medicine, Department of Pharmacology, Uniformed Services University, Bethesda, MD 20814, United States.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Cox
authors.affiliation: Structural Biology Unit, Integrative Neuroscience Branch, NIH/NIDA-IRP, Baltimore, MD 21224, United States. Electronic address: awoods@intra.nida.nih.gov.
authors.forename: Amina S
authors.initials: AS
authors.lastname: Woods
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism*; D000070642:Brain Injuries, Traumatic / Q000000981:diagnostic imaging* / Q000378:metabolism*; D004195:Disease Models, Animal; D018450:Disease Progression; D057918:Fiducial Markers; D008055:Lipids; D008297:Male; D013058:Mass Spectrometry; D053768:Metal Nanoparticles; D017207:Rats, Sprague-Dawley; D018030:Silver Compounds; D013997:Time Factors
chemical_list: D015415:Biomarkers; D008055:Lipids; D018030:Silver Compounds
keywords: Controlled cortical impact; Lipids; Mass spectrometry imaging; Silver nanoparticles implantation; Traumatic brain injury