title: Water-Soluble Compounds from Lentinula edodes Influencing the HMG-CoA Reductase Activity and the Expression of Genes Involved in the Cholesterol Metabolism.
abstract: A water extract from Lentinula edodes (LWE) showed HMG-CoA reductase inhibitory activity but contained no statins. NMR indicated the presence of water-soluble α- and β-glucans and fucomannogalactans. Fractions containing derivatives of these polysaccharides with molecular weight down to approximately 1 kDa still retained their inhibitory activity. Once digested LWE was applied to Caco2 in transport experiments, no significant effect was noticed on the modulation of cholesterol-related gene expression. But, when the lower compartment of the Caco2 monolayer was applied to HepG2, some genes were modulated (after 24 h). LWE was also administrated to normo- and hypercholesterolemic mice, and no significant lowering of serum cholesterol levels was observed; but reduction of triglycerides in liver was observed. However, LWE supplementation modulated the transcriptional profile of some genes involved in the cholesterol metabolism similarly to simvastatin, suggesting that it could hold potential as a hypolipidemic/hypocholesterolemic extract, although further dose-dependent studies should be carried out.
authors.affiliation: Department of Production and Characterization of Novel Foods, CIAL-Research Institute in Food Science (UAM+CSIC), C/Nicolás Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid , 28049 Madrid, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Gil-Ramírez
authors.affiliation: Department of Experimental Surgery, Resarch Institute Hospital La Paz (IdiPAZ) , Paseo de la Castellana 261, 28046 Madrid, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Caz
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Parana , CP 19046 Curitiba, Parana, Brazil.
authors.forename: Fhernanda R
authors.initials: FR
authors.lastname: Smiderle
authors.affiliation: Imdea-Food Institute, CEI UAM+CSIC, Pabellón Central del Antiguo Hospital de Cantoblanco (Edificio n° 7), Crta. de Cantoblanco n° 8, 28049 Madrid, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Martin-Hernandez
authors.affiliation: Department of Experimental Surgery, Resarch Institute Hospital La Paz (IdiPAZ) , Paseo de la Castellana 261, 28046 Madrid, Spain.
authors.forename: Carlota
authors.initials: C
authors.lastname: Largo
authors.affiliation: Department of Experimental Surgery, Resarch Institute Hospital La Paz (IdiPAZ) , Paseo de la Castellana 261, 28046 Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Tabernero
authors.affiliation: Department of Production and Characterization of Novel Foods, CIAL-Research Institute in Food Science (UAM+CSIC), C/Nicolás Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid , 28049 Madrid, Spain.
authors.forename: Francisco R
authors.initials: FR
authors.lastname: Marín
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Parana , CP 19046 Curitiba, Parana, Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Department of Production and Characterization of Novel Foods, CIAL-Research Institute in Food Science (UAM+CSIC), C/Nicolás Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid , 28049 Madrid, Spain.|Imdea-Food Institute, CEI UAM+CSIC, Pabellón Central del Antiguo Hospital de Cantoblanco (Edificio n° 7), Crta. de Cantoblanco n° 8, 28049 Madrid, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Reglero
authors.affiliation: Department of Production and Characterization of Novel Foods, CIAL-Research Institute in Food Science (UAM+CSIC), C/Nicolás Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid , 28049 Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Soler-Rivas
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002784:Cholesterol / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D005643:Fucose / Q000032:analysis; D005685:Galactans / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects*; D005936:Glucans / Q000032:analysis / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000032:analysis / Q000494:pharmacology*; D006937:Hypercholesterolemia / Q000097:blood / Q000188:drug therapy; D008099:Liver / Q000737:chemistry; D008297:Male; D008358:Mannose / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D020942:Shiitake Mushrooms / Q000737:chemistry*; D012995:Solubility; D014280:Triglycerides / Q000032:analysis; D014867:Water
chemical_list: D005685:Galactans; D005936:Glucans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D014280:Triglycerides; D014867:Water; D005643:Fucose; D002784:Cholesterol; D008358:Mannose
keywords: HMG-CoA reductase; cholesterol; eritadenine; low-density array; polysaccharides; shiitake