title: MicroRNA-26a induces osteosarcoma cell growth and metastasis via the Wnt/β-catenin pathway.
abstract: MicroRNAs (miRNAs/miRs) are a type of highly conserved, small non-coding RNA that are vital to the post-transcriptional regulation of gene expression via base pairing with target mRNA 3'-untranslated regions (3'-UTRs). Several studies have indicated that the abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS). In the present study, the role of miR-26a in the progression and metastasis of OS was investigated using reverse transcription-quantitative polymerase chain reaction, a luciferase activity assay, cell viability assay,in vitromigration and invasion assays, transfection and western blot analysis. miR-26a was upregulated in OS tissues and cell lines, and the expression of miR-26a was indicated to affect the proliferation, migration and invasion of OS Saos-2 cells. At the molecular level, the results showed that glycogen synthase kinase-3β (GSK-3β) was identified as a target of miR-26a, and the ectopic expression of miR-26a inhibited GSK-3β by directly binding to the 3'-UTR. Therefore, the expression of miR-26a was negatively correlated with GSK-3β in the OS tissues. These data suggest that miR-26a is significant in the proliferation of human OS cells due to the direct regulation of Wnt/β-catenin signaling.
authors.affiliation: Department of Orthopedics, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China; Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Chun-Bao
authors.initials: CB
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Bang-Tuo
authors.initials: BT
authors.lastname: Yuan
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Xue-Zhen
authors.initials: XZ
authors.lastname: Shen
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Liu
keywords: cell proliferation; glycogen synthase kinase-3β; microRNA-26a; microRNAs; osteosarcoma