title: Characterization of Fe (III)-reducing enrichment culture and isolation of Fe (III)-reducing bacterium Enterobacter sp. L6 from marine sediment.
abstract: To enrich the Fe (III)-reducing bacteria, sludge from marine sediment was inoculated into the medium using Fe (OH)3 as the sole electron acceptor. Efficiency of Fe (III) reduction and composition of Fe (III)-reducing enrichment culture were analyzed. The results indicated that the Fe (III)-reducing enrichment culture with the dominant bacteria relating to Clostridium and Enterobacter sp. had high Fe (III) reduction of (2.73 ± 0.13) mmol/L-Fe (II). A new Fe (III)-reducing bacterium was isolated from the Fe (III)-reducing enrichment culture and identified as Enterobacter sp. L6 by 16S rRNA gene sequence analysis. The Fe (III)-reducing ability of strain L6 under different culture conditions was investigated. The results indicated that strain L6 had high Fe (III)-reducing activity using glucose and pyruvate as carbon sources. Strain L6 could reduce Fe (III) at the range of NaCl concentrations tested and had the highest Fe (III) reduction of (4.63 ± 0.27) mmol/L Fe (II) at the NaCl concentration of 4 g/L. This strain L6 could reduce Fe (III) with unique properties in adaptability to salt variation, which indicated that it can be used as a model organism to study Fe (III)-reducing activity isolated from marine environment.
authors.affiliation: Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine and Environmental Sciences, Tianjin University of Science & Technology, Tianjin 300457, PR China. Electronic address: hongyanliu1214@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine and Environmental Sciences, Tianjin University of Science & Technology, Tianjin 300457, PR China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Wang
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004754:Enterobacter / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D005947:Glucose / Q000378:metabolism; D010084:Oxidation-Reduction; D019289:Pyruvic Acid / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D004269:DNA, Bacterial; D005290:Ferric Compounds; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D019289:Pyruvic Acid; D005947:Glucose
keywords: Denaturing gradient gel electrophoresis; Enterobacter sp. L6; Fe (III) reduction; Marine sediment