title: Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation.
abstract: B-cell specific moloney leukemia virus insertion site 1 (Bmi-1) gene plays important roles in gastric cancer, but the epigenetic regulatory mechanism by microRNA (miRNA) and the functional significance of Bmi-1 inhibition in gastric cancer remains elusive. In this study, we systematically investigated the functional roles of miRNA mediated Bmi-1 suppression in gastric cancer. Our results show that the expression of miR-15a is significantly reduced in gastric cancer and the protein expression levels of Bmi-1 are inversely correlated with miR-15a (P = 0.034) in gastric cancer patient samples. Functional studies revealed that ectopic expression of miR-15a decreased Bmi-1 in gastric cancer cell lines with reduced proliferation and tumor invasion. High levels of Bmi-1 in gastric cancer patients are significantly associated with better overall survival (P = 0.024) based on the Kaplan-Meier survival analysis.
authors.affiliation: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.
authors.forename: Changping
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.|Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.|Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.affiliation: Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Fesler
authors.affiliation: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: BioGenex Inc., Fremont, CA, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Tong
authors.affiliation: Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Burke
authors.affiliation: Translational Research Laboratory, Department of Pathology, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Ju
authors.affiliation: Department of Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, China.|Jiangsu Engineering Research Center for Tumor Immunotherapy, Changzhou, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D063150:Polycomb Repressive Complex 1 / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D014176:Protein Biosynthesis; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: C069077:BMI1 protein, human; D014408:Biomarkers, Tumor; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1
keywords: Bmi-1; gastric cancer; miR-15a; survival