title: Identification of novel CAP superfamily protein members of Echinococcus granulosus protoscoleces.
abstract: Echinoccocus granulosus is the causative agent of Cyst Echinococcosis, a zoonotic infection affecting humans and livestock representing a public health and an economic burden for several countries. Despite decades of investigation an effective vaccine still remains to be found. Parasitic cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 proteins (CAPs) have been proposed as vaccine candidates against helmith's infection. In this work we have identified two novel proteins of this superfamily expressed at the protoescoleces larval stage named EgVAL1 and EgVAL2. The open reading frame sequences were deduced. The aminoacidic sequence was analyzed and confronted against already known vertebrate' and helminth's proteins sequences in order to infer putative functions. Immunolocalization studies were also performed. The obtained data supported by immunolocalization studies and homology models suggest that these proteins could be involved in protease activity inhibition.
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: ceciliasilvarrey@gmail.com.
authors.forename: María Cecilia
authors.initials: MC
authors.lastname: Silvarrey
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: pabloeche00@hotmail.com.
authors.forename: Soledad
authors.initials: S
authors.lastname: Echeverría
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: aliscc@gmail.com.
authors.forename: Alicia
authors.initials: A
authors.lastname: Costábile
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: castillo@fcien.edu.uy.
authors.forename: Estela
authors.initials: E
authors.lastname: Castillo
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: margot.paulino@gmail.com.
authors.forename: Margot
authors.initials: M
authors.lastname: Paulino
authors.affiliation: Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: aesteves@fcien.edu.uy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Esteves
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D048209:Echinococcus granulosus / Q000737:chemistry*; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D007814:Larva / Q000737:chemistry*; D010802:Phylogeny
chemical_list: D015801:Helminth Proteins
keywords: CAP superfamily; Echinococcus granulosus; Proteins