title: Enhancement of Biodegradable Plastic-degrading Enzyme Production from Paraphoma-like Fungus, Strain B47-9.
abstract: To improve the productivity of Paraphoma-like fungal strain B47-9 for biodegradable plastic (BP)-degrading enzyme (PCLE), the optimal concentration of emulsified poly(butylene succinate-co-adipate) (PBSA) in the medium was determined. Emulsified PBSA was consumed as a sole carbon source and an inducer of PCLE production by strain B47-9. Among the various concentrations of emulsified PBSA [0.09-0.9% (w/v)] used in flask cultivation, 0.27% yielded the maximum enzyme activity within a short cultivation period. To evaluate the residual concentration of emulsified PBSA in culture, emulsified PBSA in aliquots of culture supernatant was digested in vitro, and the concentration of released monomerised succinic acid was determined. Regardless of the initial concentration of emulsified PBSA in medium, PCLE activity was detected after residual succinic acid decreased below 0.04 mg/mL in culture broth. Jarfermentation was performed at a 0.27% PBSA concentration. Among the various airflow rates tested, 1 LPM resulted in a PCLE production rate of 1.0 U/mL/day. The enzyme activity in the resulting culture filtrate (4.2 U/2 mL) was shown to degrade commercial BP films (1 × 1 cm, 20 µm thickness) within 8 hours.
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES).
authors.forename: Yuka
authors.initials: Y
authors.lastname: Sameshima-Yamashita
authors.forename: Motoo
authors.initials: M
authors.lastname: Koitabashi
authors.forename: Wataru
authors.initials: W
authors.lastname: Tsuchiya
authors.forename: Ken
authors.initials: K
authors.lastname: Suzuki
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Shinozaki
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamamoto-Tamura
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kitamoto
mesh_terms: D000272:Adipates / Q000378:metabolism*; D001203:Ascomycota / Q000201:enzymology*; D063566:Biodegradable Plastics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D003470:Culture Media; D004655:Emulsions; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis*; D013386:Succinates / Q000378:metabolism*
chemical_list: D000272:Adipates; D063566:Biodegradable Plastics; D003470:Culture Media; D004655:Emulsions; D005656:Fungal Proteins; D013386:Succinates; C437084:poly(tetramethylene succinate-co-tetramethylene adipate); D002265:Carboxylic Ester Hydrolases; C019952:cutinase