title: Radix Tetrastigma hemsleyani flavone inhibits proliferation, migration, and invasion of human lung carcinoma A549 cells.
abstract: Radix Tetrastigma hemsleyani flavone (RTHF) is widely used as a traditional herb and has detoxification and anti-inflammatory effects. In this study, we investigated the potential effects of RTHF on the growth and metastasis of human lung adenocarcinoma A549 cells and evaluated its mechanisms. A549 cells were treated with RTHF at various concentrations for different periods. In vitro Cell Counting Kit-8 assay and colony formation methods showed that RTHF had dose- and time-dependent antiproliferation effects on A549 cells. A cell adhesion assay showed that RTHF decreased A549 cell adhesion in a dose-dependent manner. Cell invasion and migration were investigated using the Transwell assay and observed using an inverted microscope; the results showed that cell metastasis was significantly lower in the treatment group than that in the control group (P<0.01). Expression of metastasis-related matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) was detected by real-time polymerase chain reaction and Western blotting. The results showed that the expression of MMP-2, MMP-9, and TIMP-1 decreased, while that of TIMP-2 increased significantly in the RTHF group when compared with the results of the control group. These results show that RTHF exhibits antigrowth and antimetastasis activity in lung cancer A549 cells by decreasing the expression of MMP-2/-9 and TIMP-1 and increasing that of TIMP-2.
authors.affiliation: Department of Oncology, Tongde Hospital of Zhejiang Province, Affiliated to Zhejiang Chinese Medical University, People's Republic of China.
authors.forename: Liangrui
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Chinese Medicine, Zhejiang Academy of Traditional Chinese Medicine, People's Republic of China.
authors.forename: Junxian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Chinese Medicine, Zhejiang Academy of Traditional Chinese Medicine, People's Republic of China.
authors.forename: Kemin
authors.initials: K
authors.lastname: Wei
authors.affiliation: Department of Chinese Medicine, Zhejiang Academy of Traditional Chinese Medicine, People's Republic of China.
authors.forename: Yijuan
authors.initials: Y
authors.lastname: Hu
keywords: flavone; lung cancer; metastasis; radix Tetrastigma hemsleyani