title: Multi-input and -output logic circuits based on bioelectrocatalysis with horseradish peroxidase and glucose oxidase immobilized in multi-responsive copolymer films on electrodes.
abstract: Herein, poly(N-isopropylacrylamide-co-N,N'-dimethylaminoethylmethacrylate) copolymer films were polymerized on electrode surface with a simple one-step method, and the enzyme horseradish peroxidase (HRP) was embedded in the films simultaneously, which were designated as P(NiPAAm-co-DMEM)-HRP. The films exhibited a reversible structure change with the external stimuli, such as pH, CO2, temperature and SO4(2-), causing the cyclic voltammetric (CV) response of electroactive K3Fe(CN)6 at the film electrodes to display the corresponding multi-stimuli sensitive ON-OFF behavior. Based on the switchable CV property of the system and the electrochemical reduction of H2O2 catalyzed by HRP in the films and mediated by Fe(CN)6(3-) in solution, a 5-input/3-output logic gate was established. To further increase the complexity of the logic system, another enzyme glucose oxidase (GOD) was added into the films, designated as P(NiPAAm-co-DMEM)-HRP-GOD. In the presence of oxygen, the oxidation of glucose in the solution was catalyzed by GOD in the films, and the produced H2O2 in situ was recognized and electrocatalytically reduced by HRP and mediated by Fe(CN)6(3-). Based on the bienzyme films, a cascaded or concatenated 4-input/3-output logic gate system was proposed. The present work combined the multi-responsive interface with bioelectrocatalysis to construct cascaded logic circuits, which might open a new avenue to develop biocomputing elements with more sophisticated functions and design novel glucose biosensors.
authors.affiliation: College of Chemistry, Beijing Normal University, 19, Xinjiekouwai Street, Haidian District, Beijing 100875, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Chemistry, Beijing Normal University, 19, Xinjiekouwai Street, Haidian District, Beijing 100875, People's Republic of China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Lian
authors.affiliation: Beijing No. 55 High School, Beijing 100027, People's Republic of China.
authors.forename: Jiannan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Beijing Normal University, 19, Xinjiekouwai Street, Haidian District, Beijing 100875, People's Republic of China; Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, People's Republic of China. Electronic address: liuhongyun@bnu.edu.cn.
authors.forename: Hongyun
authors.initials: H
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000302:isolation & purification*; D005949:Glucose Oxidase / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D011108:Polymers; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose; D010100:Oxygen
keywords: Bioelectrocatalysis; Glucose oxidase; Horseradish peroxidase; Logic gate; Multi-responsive interface