title: Circulating miR-122-5p as a potential novel biomarker for diagnosis of acute myocardial infarction.
abstract: BACKGROUND
  MicroRNAs (miRNAs) play key roles in cardiac development, and the expression of miRNAs is altered in the diseased heart. The aim of this study was to explore the value of circulating microRNA-122-5p (miR-122-5p) as a potential biomarker for acute myocardial infarction (AMI).
  
  
  METHODS
  Plasma samples from 50 patients with AMI and 39 healthy adults (non-AMI controls) were collected. The abundance of circulating miR-122-5p was measured using quantitative real-time PCR (qRT-PCR). The cTnI concentrations of these samples were analyzed by ELISA.
  
  
  RESULTS
  Our findings revealed that circulating miR-122-5p expression were increased in AMI patients at 4 h, 8 h, 12 h, and 24 h by contrast to those non-AMI controls and displayed similar trends to that of cTnI concentrations in AMI patients. Further study showed that there is a high correlation between circulating miR-122-5p and cTnI concentrations. At last, the receiver operating characteristic (ROC) curve was performed and showed that circulating miR-122-5p had considerable diagnostic accuracy for AMI with an area under curve (AUC) of 0.855.
  
  
  CONCLUSIONS
  Our results implied that circulating miR-122-5p could be a potential biomarker for AMI.
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Xin-Liang
authors.initials: XL
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Xue-Li
authors.initials: XL
authors.lastname: Lu
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Cheng-Yun
authors.initials: CY
authors.lastname: Yan
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Qi-Lin
authors.initials: QL
authors.lastname: Wan
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Guan-Chang
authors.initials: GC
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, Huaihe Hospital of Henan University Kaifeng 475000, Henan Province, China.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Li
mesh_terms: D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D011237:Predictive Value of Tests; D011379:Prognosis; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D013997:Time Factors; D019210:Troponin I / Q000097:blood; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D019210:Troponin I
keywords: Acute myocardial infarction; biomarker; diagnosis; miR-122-5p