title: Effect of nano-sized, elemental selenium supplement on the proteome of chicken liver.
abstract: The nano-sized (100-500 nm) selenium has higher bioavailability and relatively lower toxicity compared to other selenium forms. The objective of the present study was to compare liver proteome profiles of broiler chicken fed with control diet without Se supplementation and diet supplemented with nano-Se with 4.25 mg/kg DM. Differential proteome analyses were performed by two-dimensional gel electrophoresis (2D-PAGE) followed by tryptic digestion and protein identification by liquid chromatography-mass spectrometry (LC-MS). Seven hundred and eight spots were detected, and 18 protein spots showed significant difference in their intensity (p < 0.05) between the two groups. In response to nano-Se supplementation, the expression of 8 proteins was higher, and 5 proteins were lower in nano-Se supplemented group compared to control group. The functions of the differentially expressed proteins indicate that the high dose of selenium supplementation induced a dietary stress. Selenium supplementation may influence the metabolism of fatty acids and carbohydrates and antioxidant system, and increase the quantity of cytoskeletal actin and the expression of actin regulatory protein as well.
authors.affiliation: Department of Animal Science, University of Debrecen, Debrecen, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Gulyas
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Csosz
authors.affiliation: Department of Animal Science, University of Debrecen, Debrecen, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Prokisch
authors.affiliation: Department of Animal Science, University of Debrecen, Debrecen, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Javor
authors.affiliation: Department of Nutrition, Szent Istvan University, Godollo, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Mezes
authors.affiliation: Department of Nutrition, Szent Istvan University, Godollo, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Erdelyi
authors.affiliation: Department of Nutrition, Szent Istvan University, Godollo, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Balogh
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors.forename: T
authors.initials: T
authors.lastname: Janaky
authors.affiliation: Department of Medical Chemistry, Faculty of Medicine, University of Szeged, Szeged, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Szabo
authors.affiliation: Department of Animal Science, University of Debrecen, Debrecen, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Simon
authors.affiliation: Department of Animal Science, University of Debrecen, Debrecen, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Czegledi
mesh_terms: D000818:Animals; D002645:Chickens; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D053758:Nanoparticles / Q000008:administration & dosage*; D020543:Proteome; D012643:Selenium / Q000008:administration & dosage* / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D020543:Proteome; D012643:Selenium
keywords: Two-Dimensional Polyacrylamide Gel Electrophoresis; chicken; liver; protein expression; selenium