title: Identification of polymorphism in fatty acid binding protein 3 (FABP3) gene and its association with milk fat traits in riverine buffalo (Bubalus bubalis).
abstract: The fatty acid binding protein 3 (FABP3) gene, known to be associated with fat percentage of milk and meat in bovines, was screened among swamp and riverine buffaloes for polymorphism detection and further association with milk fat contents. An SNP g.307C > T was identified in the intron 2 (+53 exon 2) region of FABP3 gene of Indian buffaloes. The SNP identified was genotyped in 692 animals belonging to 15 riverine, swamp and hybrid (riverine × swamp) buffalo populations of diverse phenotypes and utilities, by PCR-RFLP. A marked contrast was observed between the C and T allele frequencies in three types of buffaloes. The frequency of C allele ranged from 0.67 to 0.96 in pure swamp buffalo populations, with the highest in Mizoram (0.96). Whereas the frequency of T allele was high across all the Indian riverine buffalo breeds, ranging from 0.57 to 0.96. None of the genotypes at FABP3 g.307C > T locus was found to have significant association with milk fat and other production traits in Mehsana dairy buffalo breed. Our study revealed marked differences in the allele frequencies between riverine and swamp buffaloes at FABP3 g.307C > T locus, without any significant association with different milk traits in riverine buffaloes.
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.|Immune regulation, WPI-IFREC, Osaka University, Osaka, Japan.
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Dubey
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.|Omics Application Technology Group, RIKEN Center for Life Science Technologies, Yokohama, Japan.
authors.forename: Shubham
authors.initials: S
authors.lastname: Goyal
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.
authors.forename: Shailendra Kumar
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.
authors.forename: Reena
authors.initials: R
authors.lastname: Arora
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.
authors.forename: Manishi
authors.initials: M
authors.lastname: Mukesh
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.
authors.forename: Saket Kumar
authors.initials: SK
authors.lastname: Niranjan
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India.|Animal Production and Health Section, International Atomic Energy Agency, Vienna, Austria.
authors.forename: Periasamy
authors.initials: P
authors.lastname: Kathiravan
authors.affiliation: National Bureau of Animal Genetic Resources, Karnal, 132001, Haryana, India. katariaranji@yahoo.co.in.|Animal Biotechnology Division, National Bureau of Animal Genetic Resources, P.O. Box 129, GT Road By-Pass, Karnal, 132001, Haryana, India. katariaranji@yahoo.co.in.
authors.forename: Ranjit Singh
authors.initials: RS
authors.lastname: Kataria
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000097:blood / Q000235:genetics* / Q000502:physiology; D003612:Dairying; D004041:Dietary Fats / Q000032:analysis*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D007194:India; D008892:Milk / Q000737:chemistry*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D053833:Wetlands
chemical_list: D004041:Dietary Fats; D050556:Fatty Acid-Binding Proteins
keywords: Association; Buffalo; FABP3; Milk fat trait; Polymorphism