title: Identification and antimicrobial susceptibility testing of positive blood culture isolates from briefly incubated solid medium cultures.
abstract: BACKGROUND
  Mass spectrometry Matrix-Assisted Laser Desorption-Ionization Time-of-Flight (MALDI-TOF) helps in the rapid identification of microorganisms causing blood stream infection. Rapid and reliable methods are required to decrease the turnaround time for reporting antimicrobial susceptibility results from blood culture isolates.
  
  
  METHODS
  An evaluation was performed on the reliability of a method for antimicrobial susceptibility testing of positive blood culture isolates from briefly incubated solid medium cultures.
  
  
  RESULTS
  The agreement between the evaluated and standard methods was 99.3%. The major and minor error rates were 0.4% and 0.3%, respectively, and no very major errors were observed.
  
  
  CONCLUSIONS
  The inoculation of briefly incubated solid medium cultures into antimicrobial susceptibility testing panels is an easy and reliable technique, and helps to decrease the turnaround time for reporting antimicrobial susceptibility results of positive blood cultures.
authors.affiliation: Unidad Intercentros de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Virgen Macarena-Virgen del Rocío, Sevilla, España. Electronic address: m.ballestero.t@gmail.com.
authors.forename: Mónica
authors.initials: M
authors.lastname: Ballestero-Téllez
authors.affiliation: Unidad Intercentros de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Virgen Macarena-Virgen del Rocío, Sevilla, España.
authors.forename: Esther
authors.initials: E
authors.lastname: Recacha
authors.affiliation: Unidad Intercentros de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Virgen Macarena-Virgen del Rocío, Sevilla, España; Departamento de Microbiología, Facultad de Medicina, Universidad de Sevilla, Sevilla, España.
authors.forename: Marina
authors.initials: M
authors.lastname: de Cueto
authors.affiliation: Unidad Intercentros de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Virgen Macarena-Virgen del Rocío, Sevilla, España; Departamento de Microbiología, Facultad de Medicina, Universidad de Sevilla, Sevilla, España.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: Pascual
mesh_terms: D016470:Bacteremia / Q000097:blood* / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods; D000071997:Blood Culture; D003470:Culture Media; D006801:Humans; D008826:Microbial Sensitivity Tests; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D003470:Culture Media
keywords: Antimicrobial susceptibility; Bacteremia; Bacteriemia; Bloodculture; Hemocultivo; Matrix-Assisted Laser Desorption-Ionization Time-of-Flight; Sensibilidad a antibióticos