title: Enhancement of dopaminergic activity and region-specific activation of Nrf2-ARE pathway by intranasal supplements of testosterone propionate in aged male rats.
abstract: The potential influence of intranasal testosterone propionate (InTP) supplements on mesodopaminergic system in aged male rats was investigated by analyzing the exploratory and motor behaviors as well as dopamine neurobiochemical indices. Meanwhile, oxidative stress parameters and pathway of nuclear factor erythroid 2-related factor 2 (Nrf2)-binding antioxidant response elements (Nrf2-ARE) were examined to check whether the Nrf2-ARE pathway was involved in the InTP-induced alteration of mesodopaminergic system in aged male rats. The exploratory and motor behavioral deficits, as well as the reduced expression of dopamine, tyrosine hydroxylase, and dopamine transporter, which indicated the declined activity of mesodopaminergic system, were ameliorated in rats administered with 12-week InTP. The results indicated that chronic InTP supplements could effectively influence the brain function activity in a way opposite to the effect of aging on the mesodopaminergic system of rats. The increased levels of Nrf2, heme oxygenase-1, and
  quinone oxidoreductase-1 in the substantia nigra and ventral tegmental area, but not in the hippocampus of InTP-administered aged male rats, indicated that the ameliorative effect of InTP supplements on mesodopaminergic system might be related to the region-specific activation of the Nrf2-ARE pathway.
authors.affiliation: Department of Neurobiology, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China; Department of Human Anatomy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Human Anatomy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors.forename: Shuangcheng
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurobiology, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors.forename: Yunxiao
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Department of Neurobiology, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China; Department of Neurology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Che
authors.affiliation: Department of Human Anatomy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Cui
authors.affiliation: Institute of Basic Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: Department of Human Anatomy, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China.
authors.forename: Huixian
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Neurobiology, Hebei Medical University, Shijiazhuang, Hebei 050017, PR China. Electronic address: shigeming@163.com.
authors.forename: Geming
authors.initials: G
authors.lastname: Shi
mesh_terms: D000818:Animals; D008297:Male; D051381:Rats; D000281:Administration, Intranasal; D000375:Aging / Q000187:drug effects; D061745:Antioxidant Response Elements; D001921:Brain / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000187:drug effects; D005106:Exploratory Behavior / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D009043:Motor Activity / Q000187:drug effects; D051267:NF-E2-Related Factor 2 / Q000494:pharmacology; D013378:Substantia Nigra / Q000187:drug effects; D043343:Testosterone Propionate / Q000494:pharmacology; D017557:Ventral Tegmental Area / Q000187:drug effects
chemical_list: D004298:Dopamine; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D043343:Testosterone Propionate
keywords: Aged male rats; Mesolimbic dopaminergic system; Nigrostriatal dopaminergic system; Nrf2-ARE; Oxidative stress; Testosterone propionate