title: Hydrolyzed polyacrylamide biodegradation and mechanism in sequencing batch biofilm reactor.
abstract: An investigation was performed to study the performance of a sequencing batch biofilm reactor (SBBR) to treat hydrolyzed polyacrylamides (HPAMs) and to determine the mechanisms of HPAM biodegradation. The mechanisms for the optimized parameters that significantly improved the degradation efficiency of the HPAMs were investigated by a synergistic effect of the co-metabolism in the sludge and the enzyme activities. The HPAM and TOC removal ratio reached 54.69% and 70.14%. A significant decrease in the total nitrogen concentration was measured. The carbon backbone of the HPAMs could be degraded after the separation of the amide group according to the data analysis. The HPLC results indicated that the HPAMs could be converted to polymer fragments without the generation of the acrylamide monomer intermediate. The results from high-throughput sequencing analysis revealed proteobacterias, bacteroidetes and planctomycetes were the key microorganisms involved in the degradation.
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yan
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Lanmei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mtbao@ouc.edu.cn.
authors.forename: Mutai
authors.initials: M
authors.lastname: Bao
authors.affiliation: College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Jinren
authors.initials: J
authors.lastname: Lu
mesh_terms: D000180:Acrylic Resins / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D018441:Biofilms; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D006868:Hydrolysis; D061328:Planctomycetales / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012722:Sewage / Q000737:chemistry / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D002244:Carbon; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical; D000099166:Polyacrylamides
keywords: Enzyme activities; Hydrolyzed polyacrylamide; Key microorganisms; Sequencing batch biofilm reactor