title: Effects of Narrow-band IR-A and of Water-Filtered Infrared A on Fibroblasts.
abstract: Exposures of the skin with electromagnetic radiation of wavelengths between 670 nm and 1400 nm are often used as a general treatment to improve wound healing and reduce pain, for example, in chronic diabetic skin lesions. We investigated the effects of water-filtered infrared A (wIRA) and of narrow-band IR-A provided by a light-emitting diode LED (LED-IR-A) irradiation in vitro on 3T3 fibroblast cultures under defined conditions with and without glyoxal administration. Glyoxal triggers the formation of advanced glycation end products, thereby mimicking a diabetic metabolic state. Cell viability and apoptotic changes were determined by flow cytometry after vital staining with Annexin V, YO-PRO-1 and propidium iodide (PI), and by SubG1 assay. Mitochondrial function and oxidative stress were examined by vital staining for radical production, mitochondrial membrane potential (MMP) and the ratio of reduced-to-oxidized glutathione (GSH/GSSG). The metabolic state was monitored by a resazurin conversion assay. The numbers of apoptotic cells were reduced in cultures irradiated with wIRA or LED-IR-A. More mitochondria showed a well-polarized MMP after wIRA irradiation in glyoxal damaged cells. LED-IR-A treatment specifically restored the GSH/GSSG ratio. The immediate positive effects of wIRA and LED-IR-A observed in living cells, particularly on mitochondria, reflect the therapeutic benefits of wIRA and LED-IR-A.
authors.affiliation: Institute of Anatomy, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Lilla
authors.initials: L
authors.lastname: Knels
authors.affiliation: Institute of Anatomy, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Valtink
authors.affiliation: Charité - University Medicine Berlin, Medical Photobiology Group, Berlin, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Piazena
authors.affiliation: Institute of Anatomy, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Jamlec
authors.initials: J
authors.lastname: de la Vega Marin
authors.affiliation: Institute of Anatomy, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Gommel
authors.affiliation: Institute of Pharmacology and Toxicology, Jena University Hospital, Jena, Germany.
authors.forename: Amelie
authors.initials: A
authors.lastname: Lupp
authors.affiliation: Institute of Anatomy, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Cora
authors.initials: C
authors.lastname: Roehlecke
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany.
authors.forename: Mirko
authors.initials: M
authors.lastname: Mehner
authors.affiliation: Institute of Anatomy, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Richard H W
authors.initials: RH
authors.lastname: Funk
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D005347:Fibroblasts / Q000528:radiation effects*; D007259:Infrared Rays; D053078:Membrane Potential, Mitochondrial / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D041681:NIH 3T3 Cells; D062048:Narrow Band Imaging; D018384:Oxidative Stress; D014867:Water
chemical_list: D014867:Water