title: Anti-inflammatory and blood stasis activities of essential oil extracted from Artemisia argyi leaf in animals.
abstract: Artemisia argyi leaf is a well-known species in traditional Chinese medicine. However, the anti-inflammatory and activating blood stasis activities of its essential oil (AAEO) have not been explored in vivo. The present study measured the contents of three chemical components by gas chromatography (GC). The anti-acute inflammatory effects of AAEO were investigated in dimethyl benzene, glacial acetic acid and carrageenan-induced animals through skin administration or by oral gavage, respectively. The effects of AAEO on haemorheology were studied in a rat acute blood stasis model. The contents of eucalyptol, camphor and borneol in AAEO were 254.4, 51.6 and 58.7 mg/g, respectively. All dosages of AAEO by skin administration significantly decreased the swelling in dimethyl benzene-induced ear oedema and carrageenan-induced paw oedema, and reduced the permeability in glacial acetic acid-induced abdominal blood capillary (p < 0.01). Meanwhile, haemorheology indexes such as whole blood viscosity and the erythrocyte aggregation index significantly decreased only in the high dosage group. In addition, the effects of AAEO by oral gavage were weaker than skin administration at the medium dose in the experiments. It suggests that AAEO has better absorption bioavailability and pharmacological effects through skin administration due to the better skin permeability of essential oil than gastrointestinal absorption.
authors.affiliation: Institute for Ethical Medicine Research, School of Pharmacy, South-Central University for Nationalities, Minyuan Road 182, Hongshan District, Wuhan, Hubei, People's Republic of China.
authors.forename: Yue-Bin
authors.initials: YB
authors.lastname: Ge
authors.affiliation: Schistosomiasis Treatment Specialized Hospital, Bengzhan Road 22, Hanchuan, Hubei, People's Republic of China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
authors.affiliation: Institute for Ethical Medicine Research, School of Pharmacy, South-Central University for Nationalities, Minyuan Road 182, Hongshan District, Wuhan, Hubei, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Institute for Ethical Medicine Research, School of Pharmacy, South-Central University for Nationalities, Minyuan Road 182, Hongshan District, Wuhan, Hubei, People's Republic of China. 179616670@qq.com.
authors.forename: Xian-Ju
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: Institute for Ethical Medicine Research, School of Pharmacy, South-Central University for Nationalities, Minyuan Road 182, Hongshan District, Wuhan, Hubei, People's Republic of China.
authors.forename: Zhi-Nan
authors.initials: ZN
authors.lastname: Mei
authors.affiliation: Institute for Ethical Medicine Research, School of Pharmacy, South-Central University for Nationalities, Minyuan Road 182, Hongshan District, Wuhan, Hubei, People's Republic of China.
authors.forename: Zong-Guo
authors.initials: ZG
authors.lastname: Hong
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods*; D051379:Mice; D009822:Oils, Volatile / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile
keywords: Acute blood stasis; Anti-inflammatory; Artemisia argyi leaf; Essential oil; GC; Skin administration