title: Binding of two bacterial biofilms to dialkyl carbamoyl chloride (DACC)-coated dressings in vitro.
abstract: OBJECTIVE
  To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing in vitro.
  
  
  METHODS
  Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm.
  
  
  RESULTS
  Pseudomonas aeruginosa biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples.
  
  
  CONCLUSIONS
  Biofilms of two different test bacteria bound to dressings in vitro with the DACC-coating on the dressings enhancing the binding of MRSA biofilm.
  
  
  BACKGROUND
  This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.
authors.affiliation: At Centre for Biomedical Sciences, Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB.
authors.forename: R
authors.initials: R
authors.lastname: Cooper
authors.affiliation: At Centre for Biomedical Sciences, Cardiff School of Health Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff CF5 2YB.
authors.forename: L
authors.initials: L
authors.lastname: Jenkins
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001458:Bandages / Q000382:microbiology*; D018441:Biofilms; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D014945:Wound Healing / Q000187:drug effects*
keywords: Cutimed Sorbact; DACC; MRSA; Pseudomonas aeruginosa; biofilm; irreversible binding