title: Ascochyta blight: isolation, characterization, and development of a rapid method to detect inhibitors of the chickpea fungal pathogen Ascochyta rabiei.
abstract: Ascochyta blight is the major disease attacking chickpea (Cicer arietinum) around the world. Since its first time report of isolation in Argentina in 2012, the pathogen has caused severe economic losses and has acquired a great importance. We report here the isolation of Ascochyta rabiei from infected chickpea beans cultivated in Santa Fe, Argentina; its identification by morphological analysis and molecular biology techniques based on internal transcribed spacer (ITS) sequence alignment, its biochemical characterization regarding the capacity to produce proteinase and phospholipase enzymes, and its antifungal susceptibility to common used antifungal agents. In order to detect new inhibitors for A. rabiei from natural sources, a bioautographic method was developed. From the screening method developed, we found that extracts from cultures of Aspergillus parasiticus are active against A. rabiei.
authors.affiliation: Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002 LRK Rosario, Argentina.
authors.forename: Luciana
authors.initials: L
authors.lastname: Bahr
authors.affiliation: Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002 LRK Rosario, Argentina.
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Castelli
authors.affiliation: Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002 LRK Rosario, Argentina.
authors.forename: Melisa Isabel
authors.initials: MI
authors.lastname: Barolo
authors.affiliation: Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002 LRK Rosario, Argentina.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ruiz Mostacero
authors.affiliation: Micología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Tosello
authors.affiliation: Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002 LRK Rosario, Argentina. Electronic address: slopez@fbioyf.unr.edu.ar.
authors.forename: Silvia Noelí
authors.initials: SN
authors.lastname: López
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001118:Argentina; D001203:Ascomycota / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D001230:Aspergillus / Q000737:chemistry; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology; D029863:Cicer / Q000382:microbiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008853:Microscopy; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010935:Plant Diseases / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D001688:Biological Products; D004271:DNA, Fungal; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer
keywords: Bioautography; Cicer arietinum; Disease control; Inhibition; Natural products