title: Enhanced dark hydrogen fermentation by addition of ferric oxide nanoparticles using Enterobacter aerogenes.
abstract: Ferric oxide nanoparticles (FONPs) were used to facilitate dark hydrogen fermentation using Enterobacter aerogenes. The hydrogen yield of glucose increased from 164.5±2.29 to 192.4±1.14mL/g when FONPs concentration increased from 0 to 200mg/L. SEM images of E. aerogenes demonstrated the existence of bacterial nanowire among cells, suggesting FONPs served as electron conduits to enhance electron transfer. TEM showed cellular internalization of FONPs, indicating hydrogenase synthesis and activity was potentially promoted due to the released iron element. When further increasing FONPs concentration to 400mg/L, the hydrogen yield of glucose decreased to 147.2±2.54mL/g. Soluble metabolic products revealed FONPs enhanced acetate pathway of hydrogen production, but weakened ethanol pathway. This shift of metabolic pathways allowed more nicotinamide adenine dinucleotide for reducing proton to hydrogen.
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Richen
authors.initials: R
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Lingkan
authors.initials: L
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China; Department of Life Science and Engineering, Jining University, Jining 273155, China.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Junhu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D003624:Darkness; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects*; D005290:Ferric Compounds / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D012995:Solubility; D013213:Starch / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D006859:Hydrogen; D013213:Starch; D005947:Glucose
keywords: Enterobacter aerogenes; Ferric oxide nanoparticles; Hydrogen fermentation