title: Cytomegalovirus-based vaccine expressing Ebola virus glycoprotein protects nonhuman primates from Ebola virus infection.
abstract: Ebolaviruses pose significant public health problems due to their high lethality, unpredictable emergence, and localization to the poorest areas of the world. In addition to implementation of standard public health control procedures, a number of experimental human vaccines are being explored as a further means for outbreak control. Recombinant cytomegalovirus (CMV)-based vectors are a novel vaccine platform that have been shown to induce substantial levels of durable, but primarily T-cell-biased responses against the encoded heterologous target antigen. Herein, we demonstrate the ability of rhesus CMV (RhCMV) expressing Ebola virus (EBOV) glycoprotein (GP) to provide protective immunity to rhesus macaques against lethal EBOV challenge. Surprisingly, vaccination was associated with high levels of GP-specific antibodies, but with no detectable GP-directed cellular immunity.
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Andrea
authors.initials: A
authors.lastname: Marzi
authors.affiliation: School of Biomedical and Healthcare Sciences, University of Plymouth, Devon, United Kingdom.
authors.forename: Aisling A
authors.initials: AA
authors.lastname: Murphy
authors.affiliation: Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Friederike
authors.initials: F
authors.lastname: Feldmann
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health &Sciences University, Portland, Oregon, United States of America.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Parkins
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Elaine
authors.initials: E
authors.lastname: Haddock
authors.affiliation: Rocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Patrick W
authors.initials: PW
authors.lastname: Hanley
authors.affiliation: School of Biological Sciences, University of Plymouth, Devon, United Kingdom.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Emery
authors.affiliation: School of Medicine, University of California, Riverside, California, United States of America.
authors.forename: Flora
authors.initials: F
authors.lastname: Engelmann
authors.affiliation: School of Medicine, University of California, Riverside, California, United States of America.
authors.forename: Ilhem
authors.initials: I
authors.lastname: Messaoudi
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.affiliation: School of Biomedical and Healthcare Sciences, University of Plymouth, Devon, United Kingdom.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Jarvis
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics*; D004195:Disease Models, Animal; D004337:Drug Carriers; D046129:Ebola Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D019142:Hemorrhagic Fever, Ebola / Q000517:prevention & control*; D008253:Macaca mulatta; D008297:Male; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D046129:Ebola Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus