title: Genotypic characterization of psittacid herpesvirus isolates from Brazil.
abstract: Thirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419bp fragment of the UL16 gene, displaying high similarity to the PsHV-1 sequences available in GenBank. Comparison of the results with the literature data suggests that the 36 Brazilian isolates from this study belong to genotype 1 and serotype 1.
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270-901, Brazil. Electronic address: mmluppi@yahoo.com.br.
authors.forename: Marcela Miranda
authors.initials: MM
authors.lastname: Luppi
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Ana Paula Moreira Franco
authors.initials: AP
authors.lastname: Luiz
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Fabiana Magalhães
authors.initials: FM
authors.lastname: Coelho
authors.affiliation: Laboratório de Patologia, Departamento de Clínica e Cirurgia, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Roselene
authors.initials: R
authors.lastname: Ecco
authors.affiliation: Laboratório de Virologia Básica e Aplicada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Flávio Guimarães
authors.initials: FG
authors.lastname: da Fonseca
authors.affiliation: Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270-901, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Resende
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D001938:Brazil; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D006564:Herpesviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D010311:Parrots; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral
keywords: PCR; Psittacid herpesvirus; PstI; RFLP