title: Titanium dental implants surface-immobilized with gold nanoparticles as osteoinductive agents for rapid osseointegration.
abstract: Gold nanoparticles (GNPs) are quite attractive materials for use as osteogenic agents due to their potential effects on the stimulation of osteoblast differentiation. In this study, an osseo-integrated titanium (Ti) implant surface coated with GNPs was used for promotion of bone regeneration. We prepared a silanized Ti surface by chemical treatment of (3-Mercaptopropyl) trimethoxysilane (MPTMS) and immobilized the GNP layer (Ti-GNP) on their surfaces via Au-S bonding. The GNP layer is uniformly immobilized on the surface and the layer covers the titanium oxide surface well, as confirmed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The Ti-GNP was used to investigate the effectiveness of this system both in vitro and in vivo. The in vitro results showed that the Ti-GNP significantly enhances the osteogenic differentiation with increased mRNA expression of osteogenic differentiation specific genes in human adipose-derived stem cells (ADSCs). Furthermore, the in vivo results showed that Ti-GNP had a significant influence on the osseous interface formation. Through these in vitro and vivo tests, we found that Ti-GNP can be useful as osseo-integration inducing dental implants for formation of an osseous interface and maintenance of nascent bone formation.
authors.affiliation: Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea; Department of Mechanical and Aerospace Engineering, The George Washington University, DC 20052, USA.
authors.forename: Dong Nyoung
authors.initials: DN
authors.lastname: Heo
authors.affiliation: Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Wan-Kyu
authors.initials: WK
authors.lastname: Ko
authors.affiliation: Department of Chemical and Biomolecular Engineering and KI for Nano Century, Korea Advanced Institute Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Hak Rae
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Donghyun
authors.initials: D
authors.lastname: Lee
authors.affiliation: School of Chemical Engineering & SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, Republic of Korea.
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
authors.affiliation: Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jung Haeng
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Yi-Hyung
authors.initials: YH
authors.lastname: Woo
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, DC 20052, USA.
authors.forename: Lijie Grace
authors.initials: LG
authors.lastname: Zhang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental Hospital at Gang-dong, Seoul 134-727, Republic of Korea. Electronic address: verycutebear@hanmail.net.
authors.forename: Deok-Won
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: kwoni@khu.ac.kr.
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D015921:Dental Implants; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D016348:Osseointegration / Q000187:drug effects*; D010316:Particle Size; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015921:Dental Implants; D006046:Gold; D014025:Titanium
keywords: Bone regeneration; Dental implant; Gold nanoparticles; Osseointegration; Osteogenic differentiation; Titanium