title: A three gene-based risk score predicts prognosis of resected non-small-cell lung cancer.
abstract: BACKGROUND
  To study the prognosis-predicting value of a risk score based on phosphorylated At (p-Akt), vascular endothelial growth factor (VEGF), and Nin one binding (NOB1) expression in patients with resected non-small-cell lung cancer (NSCLC).
  
  
  METHODS
  A prospective cohort among 98 consecutive patients with resected NSCLC was conducted in 2009 to 2010. Immunohistochemistry was used in the detection of p-Akt, VEGF, and NOB1 expression. Any of three genes with positive expression was allocated a score of 1, otherwise scored 0. The risk score ranged from 0-3. Prognosis outcomes included overall survival (OS) and progression-free survival (PFS). Log-rank test and Cox hazard model were used to investigate the prognosis predicting value for the risk score.
  
  
  RESULTS
  In the 98 NSCLC tissue specimens, p-Akt, VEGF and NOB1 positive Expression rates were 42.9%, 66.3%, and 60.2%, respectively. The median for OS was 44 month, with 95% CI 35-51 months, and the median for PFS was 36 months, with 95% CI 25-49 months. Log-rank test showed OS and PFS related with TMN stage, lymph node metastasis, p-Akt expression, VEGF expression, NOB1 expression, and gene-based risk score (P<0.05). Multivariate COX analysis showed pTMN stage, lymph node metastasis, p-Akt expression, VEGF expression, and gene-based risk score were independent prognosis factors for OS and PFS. The adjusted HR for gene-based risk score with every one score increase was 1.21 [1.04-1.56] for OS and 1.19 [1.02-1.79] for PFS.
  
  
  CONCLUSIONS
  Our results suggest the risk scores based on p-Akt, VEGF, NOB1 expression can predict postoperative survival in patients with resected NSCLC.
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, P. R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Nantong University Nantong, Jiangsu Province, P. R. China.
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Chen
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, P. R. China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Gu
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, P. R. China.
authors.forename: Qing-Sheng
authors.initials: QS
authors.lastname: You
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000235:genetics / Q000556:secondary / Q000601:surgery*; D003661:Decision Support Techniques; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology / Q000601:surgery*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics; D010766:Phosphorylation; D011013:Pneumonectomy; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D011446:Prospective Studies; D051057:Proto-Oncogene Proteins c-akt / Q000032:analysis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000235:genetics; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C506664:NOB1 protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051057:Proto-Oncogene Proteins c-akt
keywords: NOB1; Non-small-cell lung cancer; VEGF; p-Akt; prognosis