title: Phage-displayed macrocyclic glycopeptide libraries.
abstract: In this report, we describe an efficient way to generate libraries of macrocyclic glycopeptides in one step by reacting phage-displayed libraries of peptides with dichloro-oxime derivatives. We showed that the reactions do not interfere with the ability of phage to replicate in bacteria. The reactions are site-selective for phage-displayed peptides and they do not modify any other proteins of phage. The technology described in this report will be instrumental for genetic selection of macrocyclic glycopeptides for diverse glycan-binding proteins.
authors.affiliation: Alberta Glycomics Centre and Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada. ratmir@ualberta.ca.
authors.forename: Simon
authors.initials: S
authors.lastname: Ng
authors.affiliation: Alberta Glycomics Centre and Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada. ratmir@ualberta.ca.
authors.forename: Ratmir
authors.initials: R
authors.lastname: Derda
mesh_terms: D006020:Glycopeptides / Q000737:chemistry*; D047028:Macrocyclic Compounds / Q000737:chemistry*; D019151:Peptide Library
chemical_list: D006020:Glycopeptides; D047028:Macrocyclic Compounds; D019151:Peptide Library