title: [Effect of Electroacupuncture Stimulation of "Neiguan" (PC 6) on Expression of Myocardial Chloride Channel-related Genes, PKC and PKG Proteins in Myocardial Ischemia Rats].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture (EA) stimulation of "Neiguan" (PC 6), etc. on expression levels of myocardial chloride (CL-) channel-related genes and intracellular protein kinase C (PKC) protein in myocardial ischemia (M) rats.
  
  
  METHODS
  Seventy SD rats were randomly divided into control group (n = 10), model group (n = 15) , Neiguan (PC 6) group (n = 15), Lieque (LU 7) group (n = 15) and non-acupoint group (n = 15). The MI model was established by i. p. of isoproterenol (ISO, a sympathomimetic beta adrenergic agonist). Electroacupuncture stimulation was applied to bilateral "Neiguan" (PC 6), "Lieque" (LU 7), or non-acupoint [the mid-point between "Tianshu" (ST 25) and "Shenque" (CV 8)] for 15 min, once a day for 7 days. Quantitative RT-PCR was employed to detect the expression levels of cystic fibrosis transmembrane conductance regulator (CFTR, a CL-channel) mRNA and chloride channel calcium-activated 1 (CLCa 1, a member of the family of calcium-activated chloride channels, CLCa) mRNA in the left cardiac ventricle tissue, and Western blot was used to detect the expression level of myocardial PKC protein of the left ventricle.
  
  
  RESULTS
  Compared with the control group, the expression levels of myocardial PKC protein, and CLCa 1 and CFTR genes were significantly increased in the model group (P<0.05). In comparison with the model group, the expression levels of myocardial PKC protein, and CFTR mRNA and CLCa 1 mRNA in the Neiguan group, and PKC protein and CLCa 1 mRNA in the Lieque and non-acupoint groups, as well as CFTR mRNA in the Lieque group were notably down-regulated (P<0.05). No significant change was found in the expression of CFTR mRNA in the non-acupoint group (P>0.05), and no significant differences were found between Neiguan and Lieque groups in the expression levels of PKC protein (P>0.05). The effects of "Neiguan" (PC 6) were obviously superior to those of non-acupoint in down-regulating myocardial PKC protein, CLCa 1 mRNA and CFTR mRNA (P<0.05).
  
  
  CONCLUSIONS
  EA stimulation of "Neiguan" (PC 6) can down-regulate the expression of myocardial PKC protein, CFTR and CLCa 1 genes in Ml rats, which may contribute to its effect in protecting rnyocardium from ischemic injury.
authors.forename: Zeng-hua
authors.initials: ZH
authors.lastname: Bai
authors.forename: Zhao-li
authors.initials: ZL
authors.lastname: Wu
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Su
authors.forename: Pei-wei
authors.initials: PW
authors.lastname: Cong
authors.forename: Yi-guo
authors.initials: YG
authors.lastname: Chen
authors.forename: Chun-ri
authors.initials: CR
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D017869:Cyclic GMP-Dependent Protein Kinases / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D015671:Electroacupuncture; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D011493:Protein Kinase C / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018118:Chloride Channels; D017869:Cyclic GMP-Dependent Protein Kinases; D011493:Protein Kinase C