title: Generation of mouse and human dendritic cells in vitro.
abstract: Dendritic cells (DC) that can orchestrate immune responses and maintain host homeostasis, are indispensable components of the immune system. Although distributed widely in many lymphoid and non-lymphoid tissues, their rarity in number has become a limiting factor for DC related research and therapies. Therefore, methods for efficiently generating large numbers of DC resembling their in vivo counterparts are urgently needed for DC related research and therapies. Herein we summarize the current methods for generating mouse and human DC in vitro and hope that these will facilitate both studies of DC biology and their clinical applications.
authors.affiliation: Institute for Immunology, Tsinghua-Peking Centre for Life Sciences, Tsinghua University School of Medicine, Beijing 100084, China.
authors.forename: Xueheng
authors.initials: X
authors.lastname: Guo
authors.affiliation: Institute for Immunology, Tsinghua-Peking Centre for Life Sciences, Tsinghua University School of Medicine, Beijing 100084, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute for Immunology, Tsinghua-Peking Centre for Life Sciences, Tsinghua University School of Medicine, Beijing 100084, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Institute for Immunology, Tsinghua-Peking Centre for Life Sciences, Tsinghua University School of Medicine, Beijing 100084, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Institute for Immunology, Tsinghua-Peking Centre for Life Sciences, Tsinghua University School of Medicine, Beijing 100084, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Lai
authors.affiliation: Institute for Immunology, Tsinghua-Peking Centre for Life Sciences, Tsinghua University School of Medicine, Beijing 100084, China. Electronic address: wuli@tsinghua.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010641:Phenotype; D013045:Species Specificity
chemical_list: D015415:Biomarkers
keywords: Dendritic cells; Flt3L; GM-CSF; in vitro culture