title: Enzyme feeding strategies for better fed-batch enzymatic hydrolysis of empty fruit bunch.
abstract: Lignin inhibitory becomes a major obstacle for enzymatic hydrolysis of empty fruit bunch conducted in high solid loading. Since current technology required high enzyme loading, surfactant application could not effectively used since it is only efficient in low enzyme loading. In addition, it will increase final operation cost. Hence, another method namely "proportional enzyme feeding" was investigated in this paper. In this method, enzyme was added to reactor proportionally to substrate addition, different from conventional method ("whole enzyme feeding") where whole enzyme was added prior to hydrolysis process started. Proportional enzyme feeding could increase enzymatic digestibility and glucose concentration up to 26% and 12% respectively, compared to whole enzyme feeding for hydrolysis duration more than 40h. If enzymatic hydrolysis was run less than 40h (25% solid loading), whole enzyme feeding is preferable.
authors.affiliation: PT Rekayasa Industri, Kalibata Timur 1 Street 36 Kalibata, Jakarta 12740, Indonesia. Electronic address: Yohanes.eko.chandra@live.com.
authors.forename: Yohanes Eko Chandra
authors.initials: YE
authors.lastname: Sugiharto
authors.affiliation: Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Ganesha 10, Bandung 40132, Indonesia.
authors.forename: Ardiyan
authors.initials: A
authors.lastname: Harimawan
authors.affiliation: Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Ganesha 10, Bandung 40132, Indonesia.
authors.forename: Made Tri Ari Penia
authors.initials: MT
authors.lastname: Kresnowati
authors.affiliation: Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Ganesha 10, Bandung 40132, Indonesia.
authors.forename: Ronny
authors.initials: R
authors.lastname: Purwadi
authors.affiliation: PT Rekayasa Industri, Kalibata Timur 1 Street 36 Kalibata, Jakarta 12740, Indonesia.
authors.forename: Rina
authors.initials: R
authors.lastname: Mariyana
authors.affiliation: PT Rekayasa Industri, Kalibata Timur 1 Street 36 Kalibata, Jakarta 12740, Indonesia.
authors.lastname: Andry
authors.affiliation: Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Ganesha 10, Bandung 40132, Indonesia.
authors.forename: Hana Nur
authors.initials: HN
authors.lastname: Fitriana
authors.affiliation: PT Rekayasa Industri, Kalibata Timur 1 Street 36 Kalibata, Jakarta 12740, Indonesia.
authors.forename: Hauna Fathmadinda
authors.initials: HF
authors.lastname: Hosen
mesh_terms: D028023:Arecaceae / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000032:analysis; D005638:Fruit / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D013227:Steam
chemical_list: D011134:Polysaccharides; D013227:Steam; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D002480:Cellulase
keywords: Empty fruit bunch; Enzymatic hydrolysis; Enzyme feeding