title: Long 5' untranslated regions regulate the RNA stability of the deep-sea filamentous phage SW1.
abstract: Virus production in the deep-sea environment has been found to be high, and viruses have been suggested to play significant roles in the overall functioning of this ecosystem. Nevertheless, little is known about these viruses, including the mechanisms that control their production, which makes them one of the least understood biological entities on Earth. Previously, we isolated the filamentous phage SW1, whose virus production and gene transcription were found to be active at low temperatures, from a deep-sea bacterium, Shewanella piezotolerans WP3. In this study, the operon structure of phage SW1 is presented, which shows two operons with exceptionally long 5' and 3' untranslated regions (UTRs). In addition, the 5'UTR was confirmed to significantly influence the RNA stability of the SW1 transcripts. Our study revealed novel regulation of the operon and led us to propose a unique regulatory mechanism for Inoviruses. This type of RNA-based regulation may represent a mechanism for significant viral production in the cold deep biosphere.
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Huahua
authors.initials: H
authors.lastname: Jian
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Guanpeng
authors.initials: G
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.|State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.|State Key Laboratory of Ocean Engineering, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Wang
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D017912:Inovirus / Q000235:genetics*; D009876:Operon; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020871:RNA Stability; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D020592:Shewanella / Q000235:genetics / Q000821:virology*; D014764:Viral Proteins / Q000502:physiology
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins