title: Molecular Detection of Tick-Borne Rickettsiales in Goats and Sheep from Southeastern China.
abstract: Members from Rickettsiales such as Anaplasma phagocytophilum, Ehrlichia chaffeensis, and some spotted fever group (SFG) Rickettsiae are important tick-borne pathogens. One hundred goats and sheep from southeastern China were examined for the presence of Anaplasma, E. chaffeensis, and SFG Rickettsiae by PCR. A. phagocytophilum, Anaplasma bovis, and Anaplasma centrale were detected in 15, 49, and 16 samples, respectively. The A. phagocytophilum and A. centrale were highly homologous to strains from Japanese sika deer and Japanese cattle, respectively, whereas a diversity of A. bovis sequences were detected. New genetic variants of Anaplasma close to A. centrale have been revealed. No Ehrlichia was detected in this study. The presence of SFG Rickettsiae was determined in 26 samples. The coinfection with more than two pathogens tested in this study was as high as 29%. This study has molecularly characterized the circulation of Anaplasma and Rickettsiae in goats and sheep in southeastern China, which highlights the risk of contracting the pathogens upon tick exposure.
authors.affiliation: 1 Department of Immunology and Pathogen Biology, Tongji University School of Medicine , Shanghai, China .
authors.forename: Yan
authors.initials: Y
authors.lastname: Ge
authors.affiliation: 1 Department of Immunology and Pathogen Biology, Tongji University School of Medicine , Shanghai, China .
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yin
authors.affiliation: 2 Department of Veterinary Biosciences, The Ohio State University , Columbus, Ohio.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Rikihisa
authors.affiliation: 1 Department of Immunology and Pathogen Biology, Tongji University School of Medicine , Shanghai, China .
authors.forename: Weiqing
authors.initials: W
authors.lastname: Pan
authors.affiliation: 3 The State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Gansu, China .
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
mesh_terms: D041081:Anaplasma phagocytophilum; D000712:Anaplasmosis / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D002681:China / Q000453:epidemiology; D018469:Ehrlichia chaffeensis; D016873:Ehrlichiosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats; D012281:Rickettsia; D012282:Rickettsia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology*; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*
keywords: Anaplasma; China; Domestic animals; Rickettsia