title: Co-fermentation of acetate and sugars facilitating microbial lipid production on acetate-rich biomass hydrolysates.
abstract: The process of lignocellulosic biomass routinely produces a stream that contains sugars plus various amounts of acetic acid. As acetate is known to inhibit the culture of microorganisms including oleaginous yeasts, little attention has been paid to explore lipid production on mixtures of acetate and sugars. Here we demonstrated that the yeast Cryptococcus curvatus can effectively co-ferment acetate and sugars for lipid production. When mixtures of acetate and glucose were applied, C. curvatus consumed both substrates simultaneously. Similar phenomena were also observed for acetate and xylose mixtures, as well as acetate-rich corn stover hydrolysates. More interestingly, the replacement of sugar with equal amount of acetate as carbon source afforded higher lipid titre and lipid content. The lipid products had fatty acid compositional profiles similar to those of cocoa butter, suggesting their potential for high value-added fats and biodiesel production. This co-fermentation strategy should facilitate lipid production technology from lignocelluloses.
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, PR China. Electronic address: gongzhiwei@wust.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Gong
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, PR China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Dalian National Laboratory for Clean Energy and Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shen
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, PR China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Yang
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, PR China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: College of Chemical Engineering and Technology, Wuhan University of Science and Technology, 947 Heping Road, Wuhan 430081, PR China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Dalian National Laboratory for Clean Energy and Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, PR China.
authors.forename: Zongbao K
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D000085:Acetates / Q000737:chemistry*; D019342:Acetic Acid / Q000737:chemistry; D056804:Biofuels; D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry; D003454:Cryptococcus / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry; D005285:Fermentation; D005947:Glucose; D005990:Glycerol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000737:chemistry; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D012441:Saccharomyces cerevisiae; D014994:Xylose / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D000085:Acetates; D056804:Biofuels; D002241:Carbohydrates; D005227:Fatty Acids; D008055:Lipids; C036909:lignocellulose; D002244:Carbon; D008031:Lignin; D014994:Xylose; D002480:Cellulase; D005947:Glucose; D005990:Glycerol; D019342:Acetic Acid
keywords: Acetic acid; Co-fermentation; Corn stover hydrolysates; Cryptococcus curvatus; Microbial lipid