title: From polyadenylation to splicing: Dual role for mRNA 3' end formation factors.
abstract: Recent genome-wide protein-RNA interaction studies have significantly reshaped our understanding of the role of mRNA 3' end formation factors in RNA biology. Originally thought to function solely in mediating cleavage and polyadenylation of mRNAs during their maturation, 3' end formation factors have now been shown to play a role in alternative splicing, even at internal introns--an unanticipated role for factors thought only to act at the 3' end of the mRNA. Here, we discuss the recent advances in our understanding of the role of 3' end formation factors in promoting global changes in alternative splicing at internal exon-intron junctions and how they act as cofactors for well known splicing regulators. Additionally, we review the mechanism by which these factors affect the recruitment of early intron recognition components to the 5' and 3' splice site. Our understanding of the roles of 3' end formation factors is still evolving, and the final picture might be more complex than originally envisioned.
authors.affiliation: a Howard Hughes Medical Institute and Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School , Worcester , MA USA.
authors.forename: Ashish
authors.initials: A
authors.lastname: Misra
authors.affiliation: a Howard Hughes Medical Institute and Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School , Worcester , MA USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Green
mesh_terms: D039223:Cleavage And Polyadenylation Specificity Factor / Q000378:metabolism; D039241:Cleavage Stimulation Factor / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D026723:Polyadenylation; D022821:RNA Splice Sites; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*
chemical_list: D039223:Cleavage And Polyadenylation Specificity Factor; D039241:Cleavage Stimulation Factor; D009687:Nuclear Proteins; D022821:RNA Splice Sites; D012333:RNA, Messenger; C101043:SYMPK protein, human
keywords: Alternative splicing; CPSF; SYMPK; mRNA 3’ end processing; polyadenylation