title: MicroRNA-183 functions as the tumor suppressor via inhibiting cellular invasion and metastasis by targeting MMP-9 in cervical cancer.
abstract: OBJECTIVE
  MicroRNAs have been reported to play an important role in the invasion and metastasis of cervical cancer. miR-183 was found to inhibit or promote the invasion and metastasis of multiple solid tumors. However, the roles of miR-183 in cervical cancer are unclear.
  
  
  METHODS
  In this study, miR-183 expression levels were measured in 53 cervical cancer and 13 normal cervical tissues by qRT-PCR. The effects of forced expression of miR-183 on cervical cancer cells invasion and metastasis were investigated using Transwell uncoated or coated with growth factor-reduced Matrigel for migration or invasion assays, respectively.
  
  
  RESULTS
  We found that miR-183 expression levels were significantly down-regulated in cervical cancer tissues compared with normal tissues (0.15±0.011 to 0.86±0.049). Ectopic expression of miR-183 resulted in the suppression of invasion and migration of cervical cancer cell lines, siha and Hela cells (p<0.0001). Bioinformatics analysis revealed that MMP-9 was the potential target of miR-183 and it was found that MMP-9 was remarkably up-regulated in cervical cancer. Furthermore, a dual-luciferase reporter assay showed that MMP-9 as a target of miR-183 (p<0.0001). The invasion and metastasis ability of siha and Hela was suppressed when MMP-9 was down-regulated in vitro (p<0.0001).
  
  
  CONCLUSIONS
  In conclusion, our study revealed that miR-183 might be a tumor suppressor via inhibiting the invasion and metastasis of cervical cancer cells through targeting MMP-9, indicating that miR-183 may be a novel potential therapeutic target for cervical cancer.
authors.affiliation: Gynaecology and Obstetrics Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Fan
authors.affiliation: Oncology Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China. Electronic address: yingw_215@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Gynaecology and Obstetrics Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Qi
authors.affiliation: Gynaecology and Obstetrics Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Urology Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China.
authors.forename: Po
authors.initials: P
authors.lastname: Xu
authors.affiliation: Gynaecology and Obstetrics Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China.
authors.forename: Xianan
authors.initials: X
authors.lastname: Yang
authors.affiliation: Gynaecology and Obstetrics Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China.
authors.forename: Ximeng
authors.initials: X
authors.lastname: Jin
authors.affiliation: Gynaecology and Obstetrics Department of the First Affiliated Hospital of Henan University of Science and Technology, No. 24 Jinghua Road, Luoyang, Henan 471003, China. Electronic address: 949898789@qq.com.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Tian
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D019295:Computational Biology; D005260:Female; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000235:genetics*; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D002583:Uterine Cervical Neoplasms / Q000401:mortality / Q000473:pathology*
chemical_list: C533449:MIRN183 microRNA, human; D035683:MicroRNAs; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Cervical cancer; Invasion; MMP-9; Metastasis; miR-183