title: Retrograde trafficking from the endosome to the trans-Golgi network mediated by the retromer is required for fungal development and pathogenicity in Fusarium graminearum.
abstract: In eukaryotes, the retromer is an endosome-localized complex involved in protein retrograde transport. However, the role of such intracellular trafficking events in pathogenic fungal development and pathogenicity remains unclear. The role of the retromer complex in Fusarium graminearum was investigated using cell biological and genetic methods. We observed the retromer core component FgVps35 (Vacuolar Protein Sorting 35) in the cytoplasm as fast-moving puncta. FgVps35-GFP co-localized with both early and late endosomes, and associated with the trans-Golgi network (TGN), suggesting that FgVps35 functions at the donor endosome membrane to mediate TGN trafficking. Disruption of microtubules with nocodazole significantly restricted the transportation of FgVps35-GFP and resulted in severe germination and growth defects. Mutation of FgVPS35 not only mimicked growth defects induced by pharmacological treatment, but also affected conidiation, ascospore formation and pathogenicity. Using yeast two-hybrid assays, we determined the interactions among FgVps35, FgVps26, FgVps29, FgVps17 and FgVps5 which are analogous to the yeast retromer complex components. Deletion of any one of these genes resulted in similar phenotypic defects to those of the ΔFgvps35 mutant and disrupted the stability of the complex. Overall, our results provide the first clear evidence of linkage between the retrograde transport mediated by the retromer complex and virulence in F. graminearum.
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Huawei
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Lin
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Ahai
authors.initials: A
authors.lastname: Chen
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Yu
authors.affiliation: Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Lu
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843-2132, USA.
authors.forename: Won-Bo
authors.initials: WB
authors.lastname: Shim
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.|Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D011992:Endosomes / Q000378:metabolism; D005670:Fusarium / Q000166:cytology / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D007425:Intracellular Membranes / Q000378:metabolism; D021381:Protein Transport; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: D033921:Vesicular Transport Proteins
keywords: FgVps35; Fusarium graminearum; pathogenicity; retrograde trafficking; retromer