title: Evaluation of Periodontal Ligament Cell Viability in Three Different Storage Media: An in Vitro Study.
abstract: OBJECTIVE
  This study was undertaken to evaluate the viability of periodontal ligament (PDL) cells of avulsed teeth in three different storage media.
  
  
  METHODS
  Forty-five premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups based on storage media used [Group I: milk (control); Group II: aloe vera (experimental); Group III: egg white (experimental)]. Following extractions, the teeth were placed in one of the three different storage media for 30 minutes, following which the scrapings of the PDL from these teeth were collected in Falcon tubes containing collagenase enzyme in 2.5 mL of phosphate buffered saline. The tubes were subsequently incubated for 30 minutes and centrifuged for five minutes at 800 rpm. The obtained PDL cells were stained with Trypan Blue and were observed under optical microscope. The percentage of viable cells was calculated.
  
  
  RESULTS
  Aloe vera showed the highest percentage of viable cells (114.3±8.0), followed by egg white (100.9±6.3) and milk (101.1±7.3).
  
  
  CONCLUSIONS
  Within the limitations of this study, it appears that aloe vera maintains PDL cell viability better than egg white or milk.
authors.affiliation: Assistant Professor, Department of Pedodontics and Preventive Dentistry, Government Dental College, Jaipur, Rajasthan, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Medical Officer, Jaipur, Rajasthan, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Professor, Department of Orthodontics and Dentofacial Orthopedics, NIMS Dental College, Jaipur, Rajasthan, India.
authors.forename: Y G
authors.initials: YG
authors.lastname: Reddy
authors.affiliation: Professor, Department of Pedodontics and Preventive Dentistry, NIMS Dental College, Jaipur, Rajasthan, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Mittal
authors.affiliation: Professor, Department of Conservative Dentistry, NIMS Dental College, Jaipur, India.
authors.forename: Vishal
authors.initials: V
authors.lastname: Agarwal
authors.affiliation: Professor, Department of Pedodontics and Preventive Dentistry, NIMS Dental College, Jaipur, Rajasthan, India.
authors.forename: Chanchal
authors.initials: C
authors.lastname: Singh
authors.affiliation: Associate Professor, Department of Public Health Dentistry, NIMS Dental College, Jaipur.
authors.forename: Amandeep
authors.initials: A
authors.lastname: Singh
keywords: Aloe vera gel; Periodontal Ligament; Tooth Avulsion