title: Overexpression of c-Myb is associated with suppression of distant metastases in colorectal carcinoma.
abstract: The MYB gene codes for the c-Myb transcription factor maintaining proliferation of colon epithelial progenitors, thus controlling colon development and homeostasis. This gene is overexpressed in early phases of colorectal cancer (CRC) tumorigenesis. The aim of this study was to examine the expression of c-Myb in CRC tissue samples both at the messenger RNA (mRNA) and protein levels and to evaluate their associations with clinicopathological characteristics in a group of 108 CRC patients. Statistically significant negative association was found between the frequency of the c-Myb-positive tumor cells assessed by immunohistochemistry and the presence of distant metastases (p < 0.01) but not tumor differentiation, tumor stage, lymph node involvement, vascular invasion, tumor localization, age, and gender of the patients. Although the c-Myb protein level in the tumor tissue correlated with its mRNA level, no significant association between MYB mRNA and any clinicopathological characteristics was observed. We conclude that albeit overexpression of c-Myb is considered as an important factor contributing to early phases of CRC tumorigenesis, it may later have negative effect on tumor cell dissemination as observed recently in breast cancer as well. Further studies are required to explain the role of c-Myb during formation of CRC distant metastases.
authors.affiliation: First Department of Pathological Anatomy, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Tichý
authors.affiliation: Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.|International Clinical Research Center, Center for Biological and Cellular Engineering, St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Lucia
authors.initials: L
authors.lastname: Knopfová
authors.affiliation: Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Jarkovský
authors.affiliation: Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.|International Clinical Research Center, Center for Biological and Cellular Engineering, St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Pekarčíková
authors.affiliation: 1st Department of Surgery, St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Veverková
authors.affiliation: 1st Department of Surgery, St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Vlček
authors.affiliation: Department of Oncology, St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Katolická
authors.affiliation: 1st Department of Surgery, St. Anne's University Hospital, Brno, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Čapov
authors.affiliation: First Department of Pathological Anatomy, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Hermanová
authors.affiliation: Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Šmarda
authors.affiliation: Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. pbenes@sci.muni.cz.|International Clinical Research Center, Center for Biological and Cellular Engineering, St. Anne's University Hospital, Brno, Czech Republic. pbenes@sci.muni.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Beneš
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020570:Genes, myb; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis
chemical_list: D009363:Neoplasm Proteins; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger; D012334:RNA, Neoplasm
keywords: Colorectal cancer; Immunohistochemistry; Metastases; c-Myb; mRNA