title: SETD7 Regulates the Differentiation of Human Embryonic Stem Cells.
abstract: The successful use of specialized cells in regenerative medicine requires an optimization in the differentiation protocols that are currently used. Understanding the molecular events that take place during the differentiation of human pluripotent cells is essential for the improvement of these protocols and the generation of high quality differentiated cells. In an effort to understand the molecular mechanisms that govern differentiation we identify the methyltransferase SETD7 as highly induced during the differentiation of human embryonic stem cells and differentially expressed between induced pluripotent cells and somatic cells. Knock-down of SETD7 causes differentiation defects in human embryonic stem cell including delay in both the silencing of pluripotency-related genes and the induction of differentiation genes. We show that SETD7 methylates linker histone H1 in vitro causing conformational changes in H1. These effects correlate with a decrease in the recruitment of H1 to the pluripotency genes OCT4 and NANOG during differentiation in the SETD7 knock down that might affect the proper silencing of these genes during differentiation.
authors.affiliation: Center for Regenerative Medicine in Barcelona, (CMRB), Barcelona, 08003, Spain.
authors.forename: Julio
authors.initials: J
authors.lastname: Castaño
authors.affiliation: Center for Regenerative Medicine in Barcelona, (CMRB), Barcelona, 08003, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Morera
authors.affiliation: Center for Regenerative Medicine in Barcelona, (CMRB), Barcelona, 08003, Spain.
authors.forename: Borja
authors.initials: B
authors.lastname: Sesé
authors.affiliation: Center for Regenerative Medicine in Barcelona, (CMRB), Barcelona, 08003, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, 28029, Spain.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Boue
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Barcelona, 08028, Spain.
authors.forename: Carles
authors.initials: C
authors.lastname: Bonet-Costa
authors.affiliation: Center for Regenerative Medicine in Barcelona, (CMRB), Barcelona, 08003, Spain.
authors.forename: Merce
authors.initials: M
authors.lastname: Martí
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Barcelona, 08028, Spain.|Departamento de Bioquímica y Biología Molecular, Facultad de Biociencias, Universidad Autónoma de Barcelona, Cerdanyola, 08193, Barcelona, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Roque
authors.affiliation: Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Barcelona, 08028, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Jordan
authors.affiliation: Center for Regenerative Medicine in Barcelona, (CMRB), Barcelona, 08003, Spain.|Spanish National Cancer Research Center (CNIO), Madrid, 28029, Spain.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Barrero
mesh_terms: D002352:Carrier Proteins; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics* / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D011485:Protein Binding; D025941:Protein Interaction Mapping
chemical_list: D002352:Carrier Proteins; D002843:Chromatin; D006657:Histones; D011495:Histone-Lysine N-Methyltransferase; C540312:SETD7 protein, human