title: Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development.
abstract: This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1- and 2-cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4-cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2- to 4-cell arrested embryos and reduce the developmental competence to blastocyst.
authors.affiliation: Kuramoto Women's Clinic, Fukuoka, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Egashira
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yamauchi
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.|Department of Genetics and Animal Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
authors.forename: Md Rashedul
authors.initials: MR
authors.lastname: Islam
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yamagami
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Suyama
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.|Animal Production Department, Kafrelsheikh University, Kafrelsheikh, Egypt.
authors.forename: El-Sharawy Mohamed
authors.initials: el-SM
authors.lastname: El-Sayed
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Tabata
authors.affiliation: Kuramoto Women's Clinic, Fukuoka, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuramoto
mesh_terms: D000818:Animals; D001757:Blastomeres; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D015726:Giant Cells; D016547:Kinesins / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000254:growth & development*; D010312:Parthenogenesis; D012333:RNA, Messenger; D034741:RNA, Small Interfering
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C527520:Kid protein, mouse; D016547:Kinesins
keywords: Kid; multinucleated blastomere; parthenogenetic embryos; siRNA