title: Facile fabrication and selective detection for cysteine of xylan/Au nanoparticles composite.
abstract: This work reported a facile and green method to prepare highly stable and uniformly distributed Au nanoparticles (AuNPs), using biopolymer xylan as stabilizing and reducing agent. Full characterizations were performed and the results revealed that AuNPs were well dispersed with the diameters of 10-30nm. The optimal condition was as follows: the ratio of xylan to HAuCl4 was 150mg:15mg, reaction temperature was 80°C and reaction time was 40min. The xylan/AuNPs composite exhibited highly selective and sensitive sensing of cysteine in aqueous solution, it could distinguish cysteine among dozens kinds of amino acids, and the limit of detection (LOD) for cysteine was calculated as 0.57μM. Besides, the xylan/AuNPs composite was applied for Cys detection in human serum. This study provides a new way for high-value utilization of the rich biomass resource and a cheap, rapid and simple method for Cys detection in real biological samples.
authors.affiliation: State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou 510640, People's Republic of China.
authors.forename: Yuqiong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou 510640, People's Republic of China.
authors.forename: Zuguang
authors.initials: Z
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou 510640, People's Republic of China; School of Life Science, Beijing Institute of Technology, No. 5 Yard, Zhong Guan Cun South Street, Haidian District, Beijing 100083, People's Republic of China.
authors.forename: Pai
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou 510640, People's Republic of China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou 510640, People's Republic of China. Electronic address: xyw@scut.edu.cn.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
mesh_terms: D003545:Cysteine / Q000032:analysis* / Q000097:blood; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D019163:Reducing Agents / Q000737:chemistry; D014990:Xylans / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D019163:Reducing Agents; D014990:Xylans; D006046:Gold; C007916:hemicellulose; D003545:Cysteine
keywords: Au nanoparticle; Cysteine; Detection; Green synthesis; Xylan