title: Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children.
abstract: Immune reactions are among the most serious complications observed after hematopoietic stem cell transplantation (HSCT) in children. Microarray technique allows for simultaneous assessment of expression of nearly all human genes. The objective of the study was to compare the whole genome expression in children before and after HSCT. A total of 33 children referred for HSCT were enrolled in the study. In 70% of the patients HSCT was performed for the treatment of neoplasms. Blood samples were obtained before HSCT and six months after the procedure. Subsequently, the whole genome expression was assessed in leukocytes using GeneChip Human Gene 1.0 ST microarray. The analysis of genomic profiles before and after HSCT revealed altered expression of 124 genes. Pathway enrichment analysis revealed upregulation of five pathways after HSCT: allograft rejection, graft-versus-host disease, type I diabetes mellitus, autoimmune thyroid disease, and viral myocarditis. The activation of those pathways seems to be related to immune reactions commonly observed after HSCT. Our results contribute to better understanding of the genomic background of the immunologic complications of HSCT.
authors.affiliation: Department of Clinical Immunology, Chair of Clinical Immunology and Transplantation, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka Street 265, 30-663 Krakow, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Skoczen
authors.affiliation: Department of Medical Genetics, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Bik-Multanowski
authors.affiliation: Department of Medical Genetics, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Jacek J
authors.initials: JJ
authors.lastname: Pietrzyk
authors.affiliation: Department of Medical Genetics, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grabowska
authors.affiliation: Department of Statistics, Cracow University of Economics, Rakowicka Street 27, 31-510 Krakow, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Fijorek
authors.affiliation: Department of Oncology and Hematology, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Strojny
authors.affiliation: Department of Oncology and Hematology, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Klus-Kwiecinska
authors.affiliation: Department of Oncology and Hematology, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
authors.forename: Walentyna
authors.initials: W
authors.lastname: Balwierz
authors.affiliation: Department of Clinical Immunology, Chair of Clinical Immunology and Transplantation, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka Street 265, 30-663 Krakow, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Siedlar