title: Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast.
abstract: Uptake of poor nitrogen sources such as branched-chain amino acids is repressed in the presence of high-quality nitrogen sources such as NH4(+) and glutamate (Glu), which is called nitrogen catabolite repression. Amino acid auxotrophic mutants of the fission yeast Schizosaccharomyces pombe were unable to grow on minimal medium containing NH4Cl or Glu even when adequate amounts of required amino acids were supplied. However, growth of these mutant cells was recovered in the vicinity of colonies of the prototrophic strain, suggesting that the prototrophic cells secrete some substances that can restore uptake of amino acids by an unknown mechanism. We identified the novel fatty acids, 10(R)-acetoxy-8(Z)-octadecenoic acid and 10(R)-hydroxy-8(Z)-octadecenoic acid, as secreted active substances, referred to as Nitrogen Signaling Factors (NSFs). Synthetic NSFs were also able to shift nitrogen source utilization from high-quality to poor nitrogen sources to allow adaptive growth of the fission yeast amino acid auxotrophic mutants in the presence of high-quality nitrogen sources. Finally, we demonstrated that the Agp3 amino acid transporter was involved in the adaptive growth. The data highlight a novel intra-species communication system for adaptation to environmental nutritional conditions in fission yeast.
authors.affiliation: Chemical Genetics Laboratory, RIKEN, Saitama, Japan.|Department of Life Sciences, Graduate School of Agriculture, Meiji University, Kanagawa, Japan.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Sun
authors.affiliation: Synthetic Organic Chemistry Laboratory, RIKEN, Saitama, Japan.|Catalysis and Integrated Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Hirai
authors.affiliation: Chemical Biology Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ueki
authors.affiliation: Chemical Genomics Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirota
authors.affiliation: Catalysis and Integrated Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Molecular Structure Characterization Unit, Technology Platform Division, RIKEN CSRS, Saitama, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Hongo
authors.affiliation: Molecular Structure Characterization Unit, Technology Platform Division, RIKEN CSRS, Saitama, Japan.
authors.forename: Takemichi
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Chemical Genetics Laboratory, RIKEN, Saitama, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hitora
authors.affiliation: Department of Public Health, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Synthetic Organic Chemistry Laboratory, RIKEN, Saitama, Japan.|Catalysis and Integrated Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Sodeoka
authors.affiliation: Chemical Biology Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.affiliation: Department of Life Sciences, Graduate School of Agriculture, Meiji University, Kanagawa, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Hamamoto
authors.affiliation: Chemical Genetics Laboratory, RIKEN, Saitama, Japan.|Chemical Genomics Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Chemical Genetics Laboratory, RIKEN, Saitama, Japan.|Chemical Genomics Research Group, RIKEN CSRS, Saitama, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yashiroda
mesh_terms: D000220:Adaptation, Biological; D026905:Amino Acid Transport Systems / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001692:Biological Transport; D057465:Catabolite Repression; D005227:Fatty Acids / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; D005227:Fatty Acids; D009584:Nitrogen