title: Sensitive determination of norepinephrine, epinephrine, dopamine and 5-hydroxytryptamine by coupling HPLC with [Ag(HIO6 )2 ](5-) -luminol chemiluminescence detection.
abstract: Based on the enhancing effects of norepinephrine (NE), epinephrine (EP), dopamine (DA) and 5-hydroxytryptamine (5-HT) on the chemiluminescence (CL) reaction between [Ag(HIO6 )2 ](5-) and luminol in alkaline solution, a high-performance liquid chromatography (HPLC) method with CL detection was explored for the sensitive determination of monoamine neurotransmitters for the first time. The UV-visible absorption spectra were recorded to study the enhancement mechanism of monoamine neurotransmitters on the CL of [Ag(HIO6 )2 ](5-) and luminol reaction. The HPLC separation of NE, EP, DA and 5-HT was achieved with isocratic elution using a mixture of aqueous 0.2% phosphoric acid and methanol (5:95, v/v) within 11.0 min. Under the optimized conditions, the detection limits of NE, EP, DA, and 5-HT were 4.8, 0.9, 1.9 and 2.3 ng/mL, respectively, corresponding to 17.6-96.0 pg for 20 μL sample injection. The recoveries of monoamine neurotransmitters in rat brain were >95.6% with the precisions expressed by RSD <5.0%. The validated HPLC-CL method was successfully applied for the quantification of NE, EP, DA and 5-HT in rat brain. This method has promising potential for some biological and clinical investigations focusing on the levels of monoamine neurotransmitters. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, 230032, People's Republic of China.|Department of pharmacy, Fuyang People's Hospital, Fuyang, 236000, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, 230032, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Xie
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, 230032, People's Republic of China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Antibiotics, Anhui Institute for Food and Drug Control, Hefei, 230051, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, 230032, People's Republic of China.
authors.forename: Qunlin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D008297:Male; D051381:Rats; D015203:Reproducibility of Results; D018030:Silver Compounds / Q000737:chemistry*
chemical_list: D015306:Biogenic Monoamines; D018030:Silver Compounds; D008165:Luminol
keywords: Ag(III); catecholamine; chemiluminescence; high-performance liquid chromatography; monoamine neurotransmitter; serotonin