title: Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro.
abstract: In this work we present biological effects of silver nanoparticles (AgNPs) synthesized by picosecond laser ablation of silver in deionized water. We examined induction of chromosomal aberrations, lymphocyte micronuclei, appearance and recovery of double strand breaks (DSBs) of DNA, cell proliferation potential, concentration of lipid peroxidation products and insulin-like growth factor 1 (ILGF-1). We found that AgNPs sized from 3 nm to 8 nm induce cell cytostasis, which is accompanied with its clastogenic action on DNA, while AgNPs, sized 2 nm behaves contrary stimulating cell proliferation by enhancing ILGF-1 concentration.
authors.affiliation: Institute of Nuclear Sciences "Vinča", University of Belgrade, Mihajla Petrovića Alasa 12-14, P.O. Box 522, 11001 Belgrade, Serbia. Electronic address: gjoksic@vinca.rs.
authors.forename: Gordana
authors.initials: G
authors.lastname: Joksić
authors.affiliation: Institute of Nuclear Sciences "Vinča", University of Belgrade, Mihajla Petrovića Alasa 12-14, P.O. Box 522, 11001 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Stašić
authors.affiliation: Institute of Nuclear Sciences "Vinča", University of Belgrade, Mihajla Petrovića Alasa 12-14, P.O. Box 522, 11001 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Filipović
authors.affiliation: Institute of Nuclear Sciences "Vinča", University of Belgrade, Mihajla Petrovića Alasa 12-14, P.O. Box 522, 11001 Belgrade, Serbia.
authors.forename: Ana Valenta
authors.initials: AV
authors.lastname: Šobot
authors.affiliation: Institute of Nuclear Sciences "Vinča", University of Belgrade, Mihajla Petrovića Alasa 12-14, P.O. Box 522, 11001 Belgrade, Serbia.
authors.forename: Milan
authors.initials: M
authors.lastname: Trtica
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D002869:Chromosome Aberrations / Q000187:drug effects; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity; D010316:Particle Size; D012834:Silver / Q000633:toxicity*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D009153:Mutagens; D017392:Thiobarbituric Acid Reactive Substances; D012834:Silver
keywords: AgNPs; Cell stasis; DNA damages; Human primary cells; Size