title: A Rotifer-Based Technique to Rear Zebrafish Larvae in Small Academic Settings.
abstract: Raising zebrafish from larvae to juveniles can be laborious, requiring frequent water exchanges and continuous culturing of live feed. This task becomes even more difficult for small institutions that do not have access to the necessary funding, equipment, or personnel to maintain large-scale systems usually employed in zebrafish husbandry. To open this opportunity to smaller institutions, a cost-efficient protocol was developed to culture Nannochloropsis to feed the halophilic, planktonic rotifer Brachionus plicatilis; the rotifers were then used to raise larval zebrafish to juveniles. By using these methods, small institutions can easily raise zebrafish embryos in a cost-efficient manner without the need to establish an extensive fish-raising facility. In addition, culturing rotifers provides a micrometazoan that serves as a model organism for teaching and undergraduate research studies for a variety of topics, including aging, toxicology, and predator-prey dynamics.
authors.affiliation: 1 Department of Biology, Ripon College , Ripon, Wisconsin.|2 Developmental and Stem Cell Biology Graduate Program, Duke University , Durham, North Carolina.
authors.forename: Raymond L
authors.initials: RL
authors.lastname: Allen
authors.affiliation: 1 Department of Biology, Ripon College , Ripon, Wisconsin.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Wallace
authors.affiliation: 1 Department of Biology, Ripon College , Ripon, Wisconsin.
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Sisson
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D012403:Rotifera / Q000254:growth & development; D015027:Zebrafish / Q000254:growth & development*