title: Cardiac troponins--Translational biomarkers in cardiology: Theory and practice of cardiac troponin high-sensitivity assays.
abstract: Tn is a unique translational biomarker in cardiology whose potential has not been diminished in the new era of high sensitive assays. cTns can be valuable markers in cardiac diseases as well as in infectious diseases and respiratory diseases. Furthermore, the role of cTns is growing in the routine evaluation of cardioxicity and in determining the efficacy/safety ratio of novel cardioprotective strategies in clinical settings. cTns can detect myocardial injury not only in a wide spectrum of laboratory animals in experimental studies in vivo, but also in isolated heart models or cardiomyocytes in vitro. The crucial issue regarding the cross-species usage of cardiac troponin investigation remains the choice of cardiac troponin testing. This review summarizes the recent proteomic data on aminoacid sequences of cTnT and cTnI in various species, as well as selected analytical characteristics of human cardiac troponin high-sensitivity assays. Due to the highly phylogenetically conserved structure of troponins, the same bioindicator can be investigated using the same method in both clinical and experimental cardiology, thus contributing to a better understanding of the pathogenesis of cardiac diseases as well as to increased effectiveness of troponin use in clinical practice. Measuring cardiac troponins using commercially available human high-sensitivity cardiac troponin tests with convenient antibodies selected on the basis of adequate proteomic knowledge can solve many issues which would otherwise be difficult to address in clinical settings for various ethical and practical reasons. Our survey could help elaborate the practical guidelines for optimizing the choice of cTns assay in cardiology.
authors.affiliation: Department of Physiology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, 500 38, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Adamcova
authors.affiliation: Department of Pharmacology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, 500 38, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Popelova-Lencova
authors.affiliation: Department of Pharmacology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, 500 38, Czech Republic.
authors.forename: Eduard
authors.initials: E
authors.lastname: Jirkovsky
authors.affiliation: Institute of Pathological Physiology, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic - Sasinkova 4, 811 08 Bratislava, Slovak Republic.|3rdDepartment of Internal Medicine, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic - Limbova 5, 833 05, Bratislava, Slovak Republic.|Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic - Vlarska 3, 833 06, Bratislava, Slovak Republic.|Center of Excellence NOREG, Slovak Republic.
authors.forename: Fedor
authors.initials: F
authors.lastname: Simko
authors.affiliation: Department of Pharmacology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, 500 38, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gersl
authors.affiliation: Department of Pharmacology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Kralove, 500 38, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Sterba
mesh_terms: D001681:Biological Assay / Q000379:methods; D015415:Biomarkers / Q000378:metabolism*; D006331:Heart Diseases / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D040901:Proteomics; D019210:Troponin I / Q000302:isolation & purification* / Q000378:metabolism; D020107:Troponin T / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D019210:Troponin I; D020107:Troponin T
keywords: cardiac biomarkers; cardiac troponin; cross-species use of cardiac troponins; high-sensitivity assay; proteomics of troponins; toxicology studies