title: Trace analysis of multi-class pesticide residues in Chinese medicinal health wines using gas chromatography with electron capture detection.
abstract: A method is described for multi-residue, high-throughput determination of trace levels of 22 organochlorine pesticides (OCPs) and 5 pyrethroid pesticides (PYPs) in Chinese medicinal (CM) health wines using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) based extraction method and gas chromatography-electron capture detection (GC-ECD). Several parameters were optimized to improve preparation and separation time while still maintaining high sensitivity. Validation tests of spiked samples showed good linearities for 27 pesticides (R = 0.9909-0.9996) over wide concentration ranges. Limits of detection (LODs) and quantification (LOQs) were measured at ng/L levels, 0.06-2 ng/L and 0.2-6 ng/L for OCPs and 0.02-3 ng/L and 0.06-7 ng/L for PYPs, respectively. Inter- and intra-day precision tests showed variations of 0.65-9.89% for OCPs and 0.98-13.99% for PYPs, respectively. Average recoveries were in the range of 47.74-120.31%, with relative standard deviations below 20%. The developed method was then applied to analyze 80 CM wine samples. Beta-BHC (Benzene hexachloride) was the most frequently detected pesticide at concentration levels of 5.67-31.55 mg/L, followed by delta-BHC, trans-chlordane, gamma-BHC, and alpha-BHC. The validated method is simple and economical, with adequate sensitivity for trace levels of multi-class pesticides. It could be adopted by laboratories for this and other types of complex matrices analysis.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, PR. China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Kong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, PR. China.
authors.forename: Qiu-Tao
authors.initials: QT
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, PR. China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Kong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, PR. China.
authors.forename: Qian-Zhen
authors.initials: QZ
authors.lastname: Liu
authors.affiliation: Department of Traditional Chinese Medicine, Air Force General Hospital, PLA, Beijing, 100142, PR. China.
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Ma
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing, 100193, PR. China.
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Yang
mesh_terms: D002849:Chromatography, Gas; D010573:Pesticide Residues / Q000032:analysis*; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
chemical_list: D010573:Pesticide Residues