title: Microfluidic devices with templated regular macroporous structures for HIV viral capture.
abstract: There is a need to develop inexpensive, portable and easy-to-use devices for viral sample processing for resource-limited settings. Here we offer a solution to efficient virus capture by incorporating macroporous materials with regular structures into microfluidic devices for affinity chromatography. Two-dimensional simulations were first conducted to investigate the effects of two structures, a nanopost array and a spherical pore network, on nanoparticle capture. Then, the two structures were created in polymers by templating anodic aluminum oxide films and 3D close-packed silica particles, respectively. When the microdevices containing functionalized porous materials were tested for human immunodeficiency virus (HIV) isolation, capture efficiencies of 80-99% were achieved under a continuous flow. Comparatively, functionalized flatbed microchannels captured around 10% of HIV particles. As the characteristic dimensions of the nanostructures are tunable, such devices can be adapted for the capture of different submicron bioparticles. The high capture efficiency and easy-to-operate nature suit the needs of resource-limited settings and may find applications in point-of-care diagnostics.
authors.affiliation: Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA 18015, USA.
authors.forename: Krissada
authors.initials: K
authors.lastname: Surawathanawises
authors.affiliation: Bioengineering Program, Lehigh University, Bethlehem, PA 18015, USA. xuc207@lehigh.edu.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Kundrod
authors.affiliation: Department of Materials Science and Engineering, Lehigh University, Bethlehem, PA 18015, USA and Bioengineering Program, Lehigh University, Bethlehem, PA 18015, USA. xuc207@lehigh.edu.
authors.forename: Xuanhong
authors.initials: X
authors.lastname: Cheng
mesh_terms: D057809:HEK293 Cells; D006678:HIV / Q000302:isolation & purification*; D006801:Humans; D056656:Lab-On-A-Chip Devices; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D016062:Porosity; D014771:Virion / Q000302:isolation & purification
chemical_list: D011137:Polystyrenes; D019904:Polymethyl Methacrylate