title: Inclusion of Strep-tag II in design of antigen receptors for T-cell immunotherapy.
abstract: Adoptive immunotherapy with genetically engineered T cells has the potential to treat cancer and other diseases. The introduction of Strep-tag II sequences into specific sites in synthetic chimeric antigen receptors or natural T-cell receptors of diverse specificities provides engineered T cells with a marker for identification and rapid purification, a method for tailoring spacer length of chimeric receptors for optimal function, and a functional element for selective antibody-coated, microbead-driven, large-scale expansion. These receptor designs facilitate cGMP manufacturing of pure populations of engineered T cells for adoptive T-cell therapies and enable in vivo tracking and retrieval of transferred cells for downstream research applications.
authors.affiliation: Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sommermeyer
authors.affiliation: Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Cabanov
authors.affiliation: Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Kosasih
authors.affiliation: Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: Tyler
authors.initials: T
authors.lastname: Hill
authors.affiliation: Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.|Department of Medicine, University of Washington, Seattle, Washington, USA.|Institute for Advanced Study, Technical University of Munich, Munich, Germany.
authors.forename: Stanley R
authors.initials: SR
authors.lastname: Riddell
mesh_terms: D000818:Animals; D058948:Cell Tracking; D005260:Female; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry* / Q000166:cytology*
chemical_list: D009842:Oligopeptides; D011948:Receptors, Antigen, T-Cell; C583458:Trp-Ser- His-Pro-Gln-Phe-Glu-Lys