title: Molecular Genotyping of HIV-1 Strains from Newly Infected Men Who Have Sex with Men in Harbin, China.
abstract: In this study, blood samples from newly HIV-1 infected men who have sex with men (MSM) were collected, and HIV-1 genotypes were identified based on gag p17-p24 and nef gene regions. We found that participants aged from 20 to 40 years old were the major infection group in Harbin. CRF01_AE was the predominant genotype, contributing to 84.7% of HIV-1 infections, followed by subtype B (4.7%), CRF07_BC (3.5%), and subtype B' (Thai B, 1.2%). Moreover, five unique recombinant forms (5.9%) were also identified, including genotypes 01B, 01C, and 01/02. The recombinant CRF01_AE/CRF02_AG was first reported in China. These results suggested that current HIV-1 genotype epidemic among MSM in Harbin is more complicated and that intersubtype recombinants have emerged. Therefore, timely regional epidemiological surveillance of HIV-1 genotype and development of prevention measures for new HIV-1 infections among MSM are quite important.
authors.affiliation: 1 Genomics Research Center (One of the State-Province Key Laboratory of Biopharmaceutical Engineering), Harbin Medical University , Harbin, China .
authors.forename: Qing-Hai
authors.initials: QH
authors.lastname: Li
authors.affiliation: 2 Department of Infectious Diseases, the Fourth Affiliated Hospital of Harbin Medical University , Harbin, China .
authors.forename: Fu-Xiang
authors.initials: FX
authors.lastname: Wang
authors.affiliation: 1 Genomics Research Center (One of the State-Province Key Laboratory of Biopharmaceutical Engineering), Harbin Medical University , Harbin, China .
authors.forename: Chao
authors.initials: C
authors.lastname: Yue
authors.affiliation: 3 Department of Microbiology, Harbin Medical University , Harbin, China .
authors.forename: Jia-Ye
authors.initials: JY
authors.lastname: Wang
authors.affiliation: 1 Genomics Research Center (One of the State-Province Key Laboratory of Biopharmaceutical Engineering), Harbin Medical University , Harbin, China .
authors.forename: Gang
authors.initials: G
authors.lastname: Jin
authors.affiliation: 4 Harbin Chest Hospital , Harbin, China .
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: 2 Department of Infectious Diseases, the Fourth Affiliated Hospital of Harbin Medical University , Harbin, China .
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.affiliation: 2 Department of Infectious Diseases, the Fourth Affiliated Hospital of Harbin Medical University , Harbin, China .
authors.forename: Yuan-Long
authors.initials: YL
authors.lastname: Lin
authors.affiliation: 1 Genomics Research Center (One of the State-Province Key Laboratory of Biopharmaceutical Engineering), Harbin Medical University , Harbin, China .
authors.forename: Hai-Ning
authors.initials: HN
authors.lastname: Li
authors.affiliation: 2 Department of Infectious Diseases, the Fourth Affiliated Hospital of Harbin Medical University , Harbin, China .
authors.forename: Shi-Yan
authors.initials: SY
authors.lastname: Feng
authors.affiliation: 1 Genomics Research Center (One of the State-Province Key Laboratory of Biopharmaceutical Engineering), Harbin Medical University , Harbin, China .
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Liu
mesh_terms: D000328:Adult; D000367:Age Factors; D002681:China / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D011995:Recombination, Genetic; D055815:Young Adult; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D054301:gag Gene Products, Human Immunodeficiency Virus; D054311:nef Gene Products, Human Immunodeficiency Virus; C514837:nef protein, Human immunodeficiency virus 1