title: Preventive Role of Endothelin Antagonist on Kidney Ischemia: Reperfusion Injury in Male and Female Rats.
abstract: BACKGROUND
  Renal ischemia/reperfusion injury (RIRI) is the most common cause of acute kidney injury. We tested the protective role of endothelin-1 receptor blocker; bosentan (BOS) in animal model of RIRI in two different genders.
  
  
  METHODS
  Male and female Wistar rats were assigned as sham operated (sham), control group (ischemia), and case group (ischemia + BOS) treated with BOS (50 mg/kg) 2 h before bilateral kidney ischemia induced by clamping renal vessels for 45 min followed by 24 h of renal reperfusion.
  
  
  RESULTS
  The RIRI significantly increased the serum levels of blood urea nitrogen and creatinine in both genders (P < 0.05). These values were significantly decreased by BOS in both genders. In male rats, the serum levels of malondialdehyde in the ischemia + BOS group were decreased significantly when compared with ischemia group (P < 0.05).
  
  
  CONCLUSIONS
  BOS can be used in both genders to attenuate kidney ischemia injury possibly due to its effect in the renal vascular system.
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Nazgol Esmalian
authors.initials: NE
authors.lastname: Afyouni
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hanieh
authors.initials: H
authors.lastname: Halili
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Moslemi
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Physiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nematbakhsh
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Talebi
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Shirdavani
authors.affiliation: Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Maleki
keywords: Bosentan; endothelin; gender; ischemia-reperfusion; kidney