title: Effect of Radix Sophorae Flavescentis on activity of CYP450 isoforms in rats.
abstract: Kushen (Radix Sophorae Flavescentis) is the dried roots of Sophora Flavescens Ait, alkaloids and flavonoids are the main active constituents of Radix Sophorae Flavescentis. The influence of Radix Sophorae Flavescentis on the activities of CYP450 isoforms CYP2B6, CYP2C19, CYP1A2, CYP2C9, CYP3A4 and CYP2D6 were evaluated by cocktail method. The rats were randomly divided into Radix Sophorae Flavescentis group and control group. The Radix Sophorae Flavescentis group rats were given 5 g/kg Radix Sophorae Flavescentis decoction by intragastric administration. The six probe drugs (bupropion, omeprazole, phenacetin, tolbutamide, midazolam and metroprolol) were given to rats through intragastric administration, and the plasma concentration were determined by UPLC-MS/MS. The result of Radix Sophorae Flavescentis group compared to control group, there were statistical pharmacokinetics difference for omeprazole, phenacetin, tolbutamide and metroprolol. It indicated that the Radix Sophorae Flavescentis may induce the activities of CYP2D6, and inhibit of CYP2C19, CYP1A2 and CYP2C9 of rats. As other drugs are always used after Radix Sophorae Flavescentis, interactions between other drugs and Radix Sophorae Flavescentis undertake the risk of either diminished efficacy or adverse effects. This may give advising for reasonable drug use after Radix Sophorae Flavescentis.
authors.affiliation: Department of Pharmacy, Wenzhou People's Hospital Wenzhou 325000, China.
authors.forename: Lianguo
authors.initials: L
authors.lastname: Chen
authors.affiliation: The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University Wenzhou 325000, China.
authors.forename: Jinzhang
authors.initials: J
authors.lastname: Cai
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui Lishui 323000, China.
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yang
keywords: CYP450; Radix Sophorae Flavescentis; cocktail; rat