title: MALAT1 is an oncogenic long non-coding RNA associated with tumor invasion in non-small cell lung cancer regulated by DNA methylation.
abstract: MALAT1 is an important long noncoding RNA in tumor progression. Here we showed that the expression of MALAT1 was upregulated in non-small cell lung cancer cells (NSCLCs) or tissues as compared with the normal lung cell or tissues. Thus, the knockdown of MALAT1 led to decreased cell migration and invasion. Next we also found that CXCL5 as a downstream gene of MALAT1 regulated cell migration and invasion. However the regulation of MALAT1 expression was rarely known. Here we found that the treatment with SAM suppressed of MALAT1 expression. Finally, we showed that the methylated forms of MALAT1 promoter in lung cancer cells or tissues decreased compared with normal lung cells or tissues. These demonstrated that the expression of MALAT1 was dependent on the methylation. Overall, our findings illuminate the oncogenic function of MALAT1 which is regulated by DNA methylation that might provide potential clinical application in NSCLC.
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital Tianjin, P. R. China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Guo
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital Tianjin, P. R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital Tianjin, P. R. China.
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General HospitalTianjin, P. R. China; Department of Lung Cancer Surgery, Tianjin Medical University General HospitalTianjin, P. R. China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General HospitalTianjin, P. R. China; Department of Lung Cancer Surgery, Tianjin Medical University General HospitalTianjin, P. R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Li
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D054365:Chemokine CXCL5 / Q000235:genetics / Q000378:metabolism; D018899:CpG Islands; D019175:DNA Methylation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D012436:S-Adenosylmethionine
keywords: CXCL5; MALAT1; cellular migration and invasion; methylation; non-small cell lung cancer