title: In Situ Investigation of Peptide-Lipid Interaction Between PAP248-286 and Model Cell Membranes.
abstract: Sum frequency generation vibrational spectroscopy (SFG) was utilized to investigate the interaction between PAP248-286 and the two lipid bilayer systems. The present study also provides spectroscopic evidence to confirm that, although PAP248-286 is unable to penetrate into the hydrophobic core of the lipid bilayers, it is capable of interacting more intimately with the fluid-phase POPG/POPC than with the gel-phase DPPG/DPPC lipid bilayer. The helical structure content of lipid-bound PAP248-286 was also observed to be high, in contrast to the results previously reported using nuclear magnetic resonance (NMR). Collectively, our SFG data suggest that lipid-bound PAP248-286 actually resembles its structure in 50 % 2,2,2-trifluoroethanol better than the structure when the peptide binds to SDS micelles. This present study questions the use of SDS micelles as the model membrane for NMR studies of PAP248-286 due to its protein denaturing activity.
authors.affiliation: School of Chemical Engineering, The University of Queensland, Brisbane, QLD, 4072, Australia. k.nguyen9@uq.edu.au.|School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, Vietnam. k.nguyen9@uq.edu.au.
authors.forename: Khoi Tan
authors.identifier: 0000-0003-3934-0371
authors.initials: KT
authors.lastname: Nguyen
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008958:Models, Molecular; D008968:Molecular Conformation; D010455:Peptides / Q000737:chemistry*; D010715:Phosphatidylglycerols
chemical_list: D008051:Lipid Bilayers; D008055:Lipids; D008823:Micelles; D010455:Peptides; D010715:Phosphatidylglycerols; C030345:1,2-dipalmitoylphosphatidylglycerol
keywords: Gel-phase and fluid-phase model lipid bilayers; PAP248–286; Peptide conformation; SDS micelles