title: [Effect of Peimine on ERCC1 mRNA and LRP Expressions of A549/DDP Multidrug Resistance Cell Line].
abstract: OBJECTIVE
  To explore the effect of peimine on excision repair cross-complementation 1 (ERCC1) mRNA and lung resistant protein (LRP) expressions in A549/cisplatin (DDP) multidrug resistance (MDR) cell line.
  
  
  METHODS
  Lung cancer A549/DDP cells were cultured in vitro.Cells at logarithmic growth phase were divided into 4 groups, i.e., the blank control group, the DDP group, the ligustrazine group (DDP+ligustrazine), the peimine group (DDP + peimine). After 48-h drug action, ERCC1 mRNA expression was detected by RT-PCR and LRP expression detected by cell immunofluorescence.
  
  
  RESULTS
  There was no statistical difference in expression levels of ERCC1 mRNA and LRP between the DDP group and the blank control group (P > 0.05). Compared with the DDP group, expression levels of ERCC1 mRNA and LRP obviously decreased in the ligustrazine group and the peimine group (P < 0.05). They were obviously lower in the peimine group than in the ligustrazine group (P < 0.05).
  
  
  CONCLUSIONS
  Peimine could reverse MDR of A549/DDP cell line. Its mechanism might be associated with down-regulating ERCC1 mRNA and LRP expression levels.
authors.forename: Xiao-yong
authors.initials: XY
authors.lastname: Tang
authors.forename: Ying-xue
authors.initials: YX
authors.lastname: Tang
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Han
mesh_terms: D045744:Cell Line, Tumor; D002595:Cevanes / Q000494:pharmacology*; D002945:Cisplatin; D004268:DNA-Binding Proteins / Q000235:genetics*; D015536:Down-Regulation; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004720:Endonucleases / Q000235:genetics*; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics*; D008175:Lung Neoplasms; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002595:Cevanes; D004268:DNA-Binding Proteins; C475476:LRP1 protein, human; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D012333:RNA, Messenger; C014242:verticine; C071447:ERCC1 protein, human; D004720:Endonucleases; D002945:Cisplatin