title: Genetic association of G-607C Located at wnt10b promoter with bi-sup type among Korean cerebral infarction patients.
abstract: Obesity is a disease threatening health and is known one of risk factors causing chronic disease. In Traditional Korean Medicine, bi-sup is casus of obesity. Wnt10b has been indicated as a potential regulator of adipogenesis in vivo and in vitro models of obesity. To analyze the distribution of wnt10b polymorphism between bi-sup group and non-bi-sup group in Korean elder subjects with cerebral infarction (CI). The study group was composed of patients with CIwho were admitted to one of the thirteen Korean oriental medical hospitals participating in this study from 2009 to 2010. A total of 670 CI patients, including 416 with bi-sup group and 254 with non-bi-sup group, participated in this study. Genotype of G-607C was conducted by primer extension using TaqManprobe and five percent of subjects were re-genotyped by direct sequencing to confirm the accuracy of the genotyping. The association of the SNP with the bi-sup group versus non-bi-sup group was performed by multiple logistic regression. Frequency of C allele in bi-sup was 45.75% which was significantly lower than 56.69% in non-bi-sup (P=0.0043, OR=0.628 [0.453-0.864]). Subjects with GC or CC type in bi-sup was also 72.36%, that was also small compared with 78.35% in non-bi-sup (P=0.0467, OR=0.675 [0.458-0.994]). These results suggest that G-607C might be used as a diagnostic genetic marker for bi-sup in stroke patients and in the development of personalized medical care.
authors.affiliation: KM Fundamental Research Division, Korea Institute of Oriental Medicine 1672 Yuseongdaero, Yuseong-gu, Daejeon, South Korea.
authors.forename: Mi Mi
authors.initials: MM
authors.lastname: Ko
authors.affiliation: Clinical Research Division, Korea Institute of Oriental Medicine 1672 Yuseongdaero, Yuseong-gu, Daejeon, South Korea.
authors.forename: Myeong Soo
authors.initials: MS
authors.lastname: Lee
authors.affiliation: KM Fundamental Research Division, Korea Institute of Oriental Medicine 1672 Yuseongdaero, Yuseong-gu, Daejeon, South Korea.
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Cha
keywords: G-607C; bi-sup; cerebral infarction; obesity; stroke; traditional Korean medicine; wnt10b