title: First structure of archaeal branched-chain amino acid aminotransferase from Thermoproteus uzoniensis specific for L-amino acids and R-amines.
abstract: The gene TUZN1299 from the genome of the hyperthermophilic archaeon Thermoproteus uzoniensis encoding a new 32.8 kDa branched-chain amino acid aminotransferase (BCAT) was expressed in Escherichia coli. The recombinant protein TUZN1299 was purified to homogeneity in the PLP-bound form. TUZN1299 was active towards branched-chain amino acids (L-Val, L-Leu, L-Ile) and showed low but detectable activity toward (R)-alpha-methylbenzylamine. The enzyme exhibits high-temperature optimum, thermal stability, and tolerance to organic solvents. The structure of an archaeal BCAT called TUZN1299 was solved for the first time (at 2.0 Å resolution). TUZN1299 has a typical BCAT type IV fold, and the organization of its active site is similar to that of bacterial BCATs. However, there are some differences in the amino acid composition of the active site.
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation. kmb@inbi.ras.ru.|NBICS Centre, National Research Centre "Kurchatov Institute", Akad. Kurchatova Sqr, 1, Moscow, 123182, Russian Federation. kmb@inbi.ras.ru.
authors.forename: Konstantin M
authors.initials: KM
authors.lastname: Boyko
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation.
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Stekhanova
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation.|NBICS Centre, National Research Centre "Kurchatov Institute", Akad. Kurchatova Sqr, 1, Moscow, 123182, Russian Federation.
authors.forename: Alena Yu
authors.initials: AY
authors.lastname: Nikolaeva
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Mardanov
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation.
authors.forename: Andrey L
authors.initials: AL
authors.lastname: Rakitin
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation.
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Ravin
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation.
authors.forename: Ekaterina Yu
authors.initials: EY
authors.lastname: Bezsudnova
authors.affiliation: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld. 2, Moscow, 119071, Russian Federation.|NBICS Centre, National Research Centre "Kurchatov Institute", Akad. Kurchatova Sqr, 1, Moscow, 123182, Russian Federation.
authors.forename: Vladimir O
authors.initials: VO
authors.lastname: Popov
mesh_terms: D000588:Amines / Q000737:chemistry / Q000378:metabolism; D000597:Amino Acids, Branched-Chain / Q000737:chemistry / Q000378:metabolism; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004795:Enzyme Stability; D013379:Substrate Specificity; D041542:Thermoproteus / Q000201:enzymology*; D000637:Transaminases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000588:Amines; D000597:Amino Acids, Branched-Chain; D019843:Archaeal Proteins; D000637:Transaminases; C019798:branched-chain-amino-acid transaminase
keywords: 3D structure; Archaea; Branched-chain amino acid aminotransferase; Selectivity; Thermostability