title: Development of a BODIPY-based ratiometric fluorescent probe for hypochlorous acid and its application in living cells.
abstract: A BODIPY-based ratiometric fluorescent probe for HOCl has been designed based on the transduction of thioether to sulfoxide function. This probe features a marked absorption and emission blue-shift upon the HOCl-promoted rapid transduction, enabling the highly selective and ratiometric detection. In addition, the probe works excellently within a wide pH range of 4-10, addressing the existing pH dependency issue. Living cells studies demonstrate that the probe is cell membrane permeable and can be employed successfully to image endogenous HOCl generation in macrophage cells.
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science & Technology, Shanghai 200237, PR China.
authors.forename: Xuzhe
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science & Technology, Shanghai 200237, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science & Technology, Shanghai 200237, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Qiang
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science & Technology, Shanghai 200237, PR China.
authors.forename: Feiyi
authors.initials: F
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science & Technology, Shanghai 200237, PR China. Electronic address: ruiwang@ecust.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory for Advanced Materials and Institute of Fine Chemicals, East China University of Science & Technology, Shanghai 200237, PR China. Electronic address: zhaocchang@ecust.edu.cn.
authors.forename: Chunchang
authors.initials: C
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000032:analysis*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D006997:Hypochlorous Acid
keywords: Fluorescent probe; High selectivity; Hypochlorous acid; Ratiometric detection; pH-independency