title: Enhanced in vitro angiogenic behaviour of human umbilical vein endothelial cells on thermally oxidized TiO2 nanofibrous surfaces.
abstract: One of the major challenges in bone grafting is the lack of sufficient bone vascularization. A rapid and stable bone vascularization at an early stage of implantation is essential for optimal functioning of the bone graft. To address this, the ability of in situ TiO2 nanofibrous surfaces fabricated via thermal oxidation method to enhance the angiogenic potential of human umbilical vein endothelial cells (HUVECs) was investigated. The cellular responses of HUVECs on TiO2 nanofibrous surfaces were studied through cell adhesion, cell proliferation, capillary-like tube formation, growth factors secretion (VEGF and BFGF), and angiogenic-endogenic-associated gene (VEGF, VEGFR2, BFGF, PGF, HGF, Ang-1, VWF, PECAM-1 and ENOS) expression analysis after 2 weeks of cell seeding. Our results show that TiO2 nanofibrous surfaces significantly enhanced adhesion, proliferation, formation of capillary-like tube networks and growth factors secretion of HUVECs, as well as leading to higher expression level of all angiogenic-endogenic-associated genes, in comparison to unmodified control surfaces. These beneficial effects suggest the potential use of such surface nanostructures to be utilized as an advantageous interface for bone grafts as they can promote angiogenesis, which improves bone vascularization.
authors.affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Ai Wen
authors.initials: AW
authors.lastname: Tan
authors.affiliation: Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia.
authors.forename: Ling Ling
authors.initials: LL
authors.lastname: Liau
authors.affiliation: Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia.
authors.forename: Kien Hui
authors.initials: KH
authors.lastname: Chua
authors.affiliation: Department of Mechanical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Roslina
authors.initials: R
authors.lastname: Ahmad
authors.affiliation: Department of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Sheikh Ali
authors.initials: SA
authors.lastname: Akbar
authors.affiliation: Department of Biomedical Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Pingguan-Murphy
mesh_terms: D042501:Angiogenic Proteins / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D015870:Gene Expression; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D018919:Neovascularization, Physiologic; D010084:Oxidation-Reduction; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry*
chemical_list: D042501:Angiogenic Proteins; D003470:Culture Media; C009495:titanium dioxide; D014025:Titanium