title: Hypothyroidism Enhanced Ectonucleotidases and Acetylcholinesterase Activities in Rat Synaptosomes can be Prevented by the Naturally Occurring Polyphenol Quercetin.
abstract: Thyroid hormones have an influence on the functioning of the central nervous system. Furthermore, the cholinergic and purinergic systems also are extensively involved in brain function. In this context, quercetin is a polyphenol with antioxidant and neuroprotective properties. This study investigated the effects of (MMI)-induced hypothyroidism on the NTPDase, 5'-nucleotidase, adenosine deaminase (ADA), and acetylcholinesterase (AChE) activities in synaptosomes of rats and whether the quercetin can prevent it. MMI at a concentration of 20 mg/100 mL was administered for 90 days in the drinking water. The animals were divided into six groups: control/water (CT/W), control/quercetin 10 mg/kg, control/quercetin 25 mg/kg, methimazole/water (MMI/W), methimazole/quercetin 10 mg/kg (MMI/Q10), and methimazole/quercetin 25 mg/kg (MMI/Q25). On the 30th day, hormonal dosing was performed to confirm hypothyroidism, and the animals were subsequently treated with 10 or 25 mg/kg quercetin for 60 days. NTPDase activity was not altered in the MMI/W group. However, treatment with quercetin decreased ATP and ADP hydrolysis in the MMI/Q10 and MMI/Q25 groups. 5'-nucleotidase activity increased in the MMI/W group, but treatments with 10 or 25 mg/kg quercetin decreased 5'-nucleotidase activity. ADA activity decreased in the CT/25 and MMI/Q25 groups. Furthermore, AChE activity was reduced in all groups with hypothyroidism. In vitro tests also demonstrated that quercetin per se decreased NTPDase, 5'-nucleotidase, and AChE activities. This study demonstrated changes in the 5'-nucleotidase and AChE activities indicating that purinergic and cholinergic neurotransmission are altered in this condition. In addition, quercetin can alter these parameters and may be a promising natural compound with important neuroprotective actions in hypothyroidism.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil. jucimarabaldissarelli@gmail.com.
authors.forename: Jucimara
authors.initials: J
authors.lastname: Baldissarelli
authors.affiliation: Conselho de Ensino e Pesquisa, Curso de Medicina, Universidade Federal de Mato Grosso, Parque Sagrada Família, Rondonópolis, MT, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: Santi
authors.affiliation: Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, Campus Ibirubá, Ibirubá, RS, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Fátima Husein
authors.initials: FH
authors.lastname: Abdalla
authors.affiliation: Universidade Federal da Fronteira Sul, Campus Chapecó, Chapecó, SC, Brazil.
authors.forename: Andréia Machado
authors.initials: AM
authors.lastname: Cardoso
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Caroline Curry
authors.initials: CC
authors.lastname: Martins
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Cidade Universitária Galeão, Rio de Janeiro, RJ, Brazil.
authors.forename: Glaecir R Mundstock
authors.initials: GR
authors.lastname: Dias
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Nicéia Spanholi
authors.initials: NS
authors.lastname: Calgaroto
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Luana Paula
authors.initials: LP
authors.lastname: Pelinson
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Karine Paula
authors.initials: KP
authors.lastname: Reichert
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Vania Lucia
authors.initials: VL
authors.lastname: Loro
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Vera Maria Melchiors
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário Camobi, Santa Maria, RS, 97105-900, Brazil. mariachitolina@gmail.com.
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D007037:Hypothyroidism / Q000188:drug therapy / Q000201:enzymology*; D008297:Male; D043583:Nucleoside-Triphosphatase / Q000378:metabolism*; D059808:Polyphenols / Q000494:pharmacology / Q000627:therapeutic use; D011794:Quercetin / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology*
chemical_list: D059808:Polyphenols; D011794:Quercetin; D000110:Acetylcholinesterase; D015720:5'-Nucleotidase; D043583:Nucleoside-Triphosphatase
keywords: Acetylcholinesterase; Ectonucleotidases; Hypothyroidism; Quercetin; Synaptosomes