title: New antimicrobial chitosan derivatives for wound dressing applications.
abstract: Chitosan is a non-toxic, biocompatible, biodegradable natural cationic polymer known for its low imunogenicity, antimicrobial, antioxidant effects and wound-healing activity. To improve its therapeutic potential, new chitosan-sulfonamide derivatives have been designed to develop new wound dressing biomaterials. The structural, morphological and physico-chemical properties of synthesized chitosan derivatives were analyzed by FT-IR, (1)H NMR spectroscopy, scanning electron microscopy, swelling ability and porosity. Antimicrobial, in vivo testing and biodegradation behavior have been also performed. The chitosan derivative membranes showed improved swelling and biodegradation rate, which are important characteristics required for the wound healing process. The antimicrobial assay evidenced that chitosan-based sulfadiazine, sulfadimethoxine and sulfamethoxazole derivatives were the most active. The MTT assay showed that some of chitosan derivatives are nontoxic. Furthermore, the in vivo study on burn wound model induced in Wistar rats demonstrated an improved healing effect and enhanced epithelialization of chitosan-sulfonamide derivatives compared to neat chitosan. The obtained results strongly recommend the use of some of the newly developed chitosan derivatives as antimicrobial wound dressing biomaterials.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
authors.forename: Oana Maria
authors.initials: OM
authors.lastname: Dragostin
authors.affiliation: Laboratory of General Biochemistry and Physical Pharmacy, Centre for Nano- and Biophotonics, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: Sangram Keshari
authors.initials: SK
authors.lastname: Samal
authors.affiliation: Polymer Chemistry & Biomaterials Research Group, Ghent University, Krijgslaan 281, S4-Bis, B-9000 Ghent, Belgium.
authors.forename: Mamoni
authors.initials: M
authors.lastname: Dash
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
authors.forename: Florentina
authors.initials: F
authors.lastname: Lupascu
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
authors.forename: Andreea
authors.initials: A
authors.lastname: Pânzariu
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
authors.forename: Cristina
authors.initials: C
authors.lastname: Tuchilus
authors.affiliation: Department of Plastic Surgery, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
authors.forename: Nicolae
authors.initials: N
authors.lastname: Ghetu
authors.affiliation: Department of Morphopathology, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
authors.forename: Mihai
authors.initials: M
authors.lastname: Danciu
authors.affiliation: Polymer Chemistry & Biomaterials Research Group, Ghent University, Krijgslaan 281, S4-Bis, B-9000 Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
authors.affiliation: Department of Plastic Surgery, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania.
authors.forename: Dragos
authors.initials: D
authors.lastname: Pieptu
authors.affiliation: Department of Physical Chemistry of Polymers, "Petru Poni" Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, 700487 Iasi, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Vasile
authors.affiliation: National Institute of Research and Development for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, Romania.
authors.forename: Rodica
authors.initials: R
authors.lastname: Tatia
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy "Grigore T. Popa" Iasi, 16 University Street, 700115 Iasi, Romania. Electronic address: lenuta.profire@umfiasi.ro.
authors.forename: Lenuta
authors.initials: L
authors.lastname: Profire
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001458:Bandages; D048271:Chitosan / Q000031:analogs & derivatives*; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D017155:Wettability; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D048271:Chitosan
keywords: Acetic acid (PubChem CID: 176); Biomaterial; Chitosan (PubChem CID: 21896651); Chitosan derivative; Chloroacetyl chloride (PubChem CID: 6577); Healing activity; Membrane; Sodium hydroxide (PubChem CID: 14798); Sodium tripolyphosphate (PubChem CID: 24455); Sulfadiazine (PubChem CID: 5215); Sulfadimethoxine (PubChem CID: 5323); Sulfamerazine (PubChem CID: 5325); Sulfamethoxazole (PubChem CID:5329); Sulfamethoxydiazine (PubChem CID: 5326); Sulfisoxazole (PubChem CID:5344)