title: In vitro study on apoptotic cell death by effective magnetic hyperthermia with chitosan-coated MnFe₂O₄.
abstract: Magnetic nanoparticles (MNPs) have been widely investigated as a hyperthermic agent for cancer treatment. In this study, thermally responsive Chitosan-coated MnFe2O4 (Chitosan-MnFe2O4) nanoparticles were developed to conduct localized magnetic hyperthermia for cancer treatment. Hydrophobic MnFe2O4 nanoparticles were synthesized via thermal decomposition and modified with 2,3-dimercaptosuccinic acid (DMSA) for further conjugation of chitosan. Chitosan-MnFe2O4 nanoparticles exhibited high magnetization and excellent biocompatibility along with low cell cytotoxicity. During magnetic hyperthermia treatment (MHT) with Chitosan-MnFe2O4 on MDA-MB 231 cancer cells, the targeted therapeutic temperature was achieved by directly controlling the strength of the external AC magnetic fields. In vitro Chitosan-MnFe2O4-assisted MHT at 42 °C led to drastic and irreversible changes in cell morphology and eventual cellular death in association with the induction of apoptosis through heat dissipation from the excited magnetic nanoparticles. Therefore, the Chitosan-MnFe2O4 nanoparticles with high biocompatibility and thermal capability can be an effective nano-mediated agent for MHT on cancer.
authors.affiliation: Center for Marine-integrated Biotechnology program (BK21 Plus), Pukyong National University, Busan, 48547, Korea.
authors.forename: Yunok
authors.initials: Y
authors.lastname: Oh
authors.forename: Nohyun
authors.initials: N
authors.lastname: Lee
authors.forename: Hyun Wook
authors.initials: HW
authors.lastname: Kang
authors.forename: Junghwan
authors.initials: J
authors.lastname: Oh
mesh_terms: D017209:Apoptosis; D001672:Biocompatible Materials / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000494:pharmacology*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D066298:In Vitro Techniques; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D017895:Manganese Compounds / Q000138:chemical synthesis / Q000494:pharmacology*; D009369:Neoplasms / Q000628:therapy
chemical_list: D001672:Biocompatible Materials; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D017895:Manganese Compounds; C551151:manganese ferrite; D048271:Chitosan