title: Synthesis of 5α-androstane-3α,17β-diol 17-O-glucuronide histaminyl conjugate for immunoassays.
abstract: Simple method of preparation of 5α-androstane-3α,17β-diol 17-O-glucuronide N-histaminyl amide was developed for the construction of immunoanalytical kit. Improved method of glucuronide derivative synthesis was used, followed by hydroxybenzotriazole-dicyclohexylcarbodiimide coupling with histamine.
authors.affiliation: Department of Chemistry of Natural Products, University of Chemistry and Technology, Technická 5, CZ 166 28 Praha 6, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Vinš
authors.affiliation: Department of Chemistry of Natural Products, University of Chemistry and Technology, Technická 5, CZ 166 28 Praha 6, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Černý
authors.affiliation: Department of Chemistry of Natural Products, University of Chemistry and Technology, Technická 5, CZ 166 28 Praha 6, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Mikšátková
authors.affiliation: Department of Chemistry of Natural Products, University of Chemistry and Technology, Technická 5, CZ 166 28 Praha 6, Czech Republic. Electronic address: Pavel.Drasar@vscht.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Drašar
mesh_terms: D015113:Androstane-3,17-diol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic; D006632:Histamine / Q000737:chemistry*; D007118:Immunoassay
chemical_list: D015113:Androstane-3,17-diol; C028895:androstane-3,17-diol glucuronide; D006632:Histamine
keywords: 5α-Androstane-3α,17β-diol; Conjugate for immunoassays; Glucuronide histaminyl conjugate; Simple efficient synthesis