title: Differential effects of a high-fat diet on serum lipid parameters and ovarian gene expression in young and aged female mice.
abstract: The aim of this study was to compare serum lipid profiles and ovarian gene expression between aged and younger female mice fed a control or a high-fat diet for 2 months. For this 16 female mice (C57BL/6) of 4 months (Young, n = 8) or 13 months (Old, n = 8) of age were used. The females were divided into four groups: (i) young females fed a normal diet; (ii) young females fed a high-fat diet; (iii) old females fed a normal diet; and (iv) old females fed a high-fat diet. Food intake was reduced (P < 0.05) in mice fed with a high-fat (2.9 ± 0.1 g) diet in comparison with control mice (3.9 ± 0.1 g). Body weight was higher for old females on the high-fat diet (35.1 ± 0.3 g) than for young females on the same diet (23.3 ± 0.4 g; P < 0.05). PON1 activity was lower in the high-fat than control diet group (114.3 ± 5.8 vs. 78.1 ± 6.0 kU/L, respectively) and was higher in older than younger females (85.9 ± 6.4 vs. 106.5 ± 5.3; P < 0.05, respectively). Females fed a high-fat diet had lower expression of Igf1 mRNA (P = 0.04). There was an interaction between age and diet for the expression of Gdf9 and Survivin, with lower expression in older females in both diets and young females that received the high-fat diet (P < 0.05). Concluding, the high-fat diet reduced the expression of ovarian Igf1 mRNA, and Gdf9 and Survivin mRNA in younger females, which can indicate lower fertility rates. High-density lipoprotein concentration and PON1 activity were higher in aged female mice.
authors.affiliation: Faculdade de Nutrição,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Driele Neske
authors.initials: DN
authors.lastname: Garcia
authors.affiliation: Faculdade de Nutrição,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Lígia Antunes
authors.initials: LA
authors.lastname: Prietsch
authors.affiliation: Faculdade de Veterinária,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Joao Alveiro Alvarado
authors.initials: JA
authors.lastname: Rincón
authors.affiliation: Faculdade de Nutrição,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Iraê de Lima
authors.initials: Ide L
authors.lastname: Moreira
authors.affiliation: Faculdade de Nutrição,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Sandra Costa
authors.initials: SC
authors.lastname: Valle
authors.affiliation: Faculdade de Nutrição,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Carlos Castilho
authors.initials: CC
authors.lastname: Barros
authors.affiliation: Faculdade de Nutrição,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Elizabete
authors.initials: E
authors.lastname: Helbig
authors.affiliation: Faculdade de Veterinária,Universidade Federal de Pelotas,Pelotas,RS,Brasil.
authors.forename: Marcio Nunes
authors.initials: MN
authors.lastname: Corrêa
authors.affiliation: Faculdade de Nutrição,Universidade Federal de Pelotas,Rua Gomes Carneiro,1 Sala 239 CEP 96010-610 Pelotas-RS,Brazil.
authors.forename: Augusto
authors.initials: A
authors.lastname: Schneider
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects*; D004435:Eating; D005260:Female; D005786:Gene Expression Regulation; D055429:Growth Differentiation Factor 9 / Q000235:genetics; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008055:Lipids / Q000097:blood*; D008810:Mice, Inbred C57BL; D010053:Ovary / Q000187:drug effects / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics; D000077022:Survivin
chemical_list: C117749:Birc5 protein, mouse; C525898:Gdf9 protein, mouse; D055429:Growth Differentiation Factor 9; D051034:Inhibitor of Apoptosis Proteins; D008055:Lipids; D012097:Repressor Proteins; D000077022:Survivin; C519530:insulin-like growth factor-1, mouse; D007334:Insulin-Like Growth Factor I; D002784:Cholesterol; D043303:Aryldialkylphosphatase; C560867:PON1 protein, mouse
keywords: AMH; FOXO3a; IGF-1; SIRT1