title: α-Ketol linolenic acid (KODA) application affects endogenous abscisic acid, jasmonic acid and aromatic volatiles in grapes infected by a pathogen (Glomerella cingulata).
abstract: Effects of α-ketol linolenic acid (KODA) application on endogenous abscisic acid (ABA), jasmonic acid (JA), and aromatic volatiles were investigated in 'Kyoho' grapes (Vitis labrusca×Vitis vinifera) infected by a pathogen (Glomerella cingulata). The expressions of 9-cis-epoxycarotenoid dioxygenase (VvNCED1), ABA 8'-hydroxylase (VvCYP707A1), lipoxygenase (VvLOX), and allene oxide synthase (VvAOS) were also examined. The grape berries were dipped in 0.1mM KODA solution before inoculation with the pathogen and stored at 25°C for 12 days. The development of infection was significantly suppressed upon KODA treatment. Endogenous ABA, JA and phaseic acid (PA) were induced in inoculated berries. KODA application before inoculation increased endogenous ABA, PA and JA through the activation of VvNCED1, VvCYP707A1 and VvAOS genes, respectively. In addition, terpenes, methyl salicylate (Me-SA) and C6-aldehydes such as (E)-2-hexenal and cis-3-hexenal associated with fungal resistance also increased in KODA-treated berries during storage. These results suggest that the synergistic effect of JA, ABA, and some aromatic volatiles induced by KODA application may provide resistance to pathogen infection in grape berries.
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Saito
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Ohkawa
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohara
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shishido
authors.affiliation: Faculty of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ikeura
authors.affiliation: Technology and Engineering Center, Shiseido Co., Kakegawa 436-0047, Japan.
authors.forename: Kazuteru
authors.initials: K
authors.lastname: Takagi
authors.affiliation: Technology and Engineering Center, Shiseido Co., Kakegawa 436-0047, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ogawa
authors.affiliation: Technology and Engineering Center, Shiseido Co., Kakegawa 436-0047, Japan.
authors.forename: Mineyuki
authors.initials: M
authors.lastname: Yokoyama
authors.affiliation: Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan. Electronic address: s-kondo@faculty.chiba-u.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kondo
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000502:physiology; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D020081:Phyllachorales / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D027843:Vitis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000502:physiology; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D000447:Aldehydes; D000975:Antioxidants; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D017962:alpha-Linolenic Acid; C586688:3-hexenal; C051750:2-hexenal; C011006:jasmonic acid; D000040:Abscisic Acid; D003577:Cytochrome P-450 Enzyme System; D049308:Dioxygenases; D008084:Lipoxygenase; C109043:9-cis-epoxy-carotenoid dioxygenase; C107203:abscisic acid 8'-hydroxylase; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase
keywords: Antioxidant activity; C(6)-aldehyde; Ketol linolenic acid; Pathogen; Vitis labrusca×Vitis vinifera