title: An Early-Stage Atherosclerosis Research Model Based on Microfluidics.
abstract: The arterial microenvironment plays a vital role in the pathology of atherosclerosis (AS). However, the interplay between the arterial microenvironment and atherogenesis remains unclear, partially due to the gap between cell culture and animal experiments. Addressing this problem, the present study reports a microfluidic AS model reconstituting early-stage AS. Physiological or AS-prone hemodynamic conditions are recapitulated on the model. The on-chip model recaptures the atherogenic responses of endothelial cells (ECs) in ways that the Petri dish could not. Significant cytotoxicity of a clinical anti-atherosclerotic drug probucol is discovered on the model, which does not appear on Petri dish but is supported by previous clinical evidence. Moreover, the anti-AS efficiency of platinum-nanoparticles (Pt-NPs) on the model shows excellent consistency with animal experiments. The early-stage AS model shows an excellent connection between Petri dish and animal experiments and highlights its promising role in bridging fundamental AS research, drug screening, and clinical trials.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No.11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, P. R. China.
authors.forename: Wenfu
authors.initials: W
authors.lastname: Zheng
authors.affiliation: College of Physics and Collaborative Innovation Center for Marine Biomass Fibers, Materials and Textiles of Shandong Province, Qingdao University, No. 308 Ningxia Road, Qingdao, 266071, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No.11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No.11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, P. R. China.
authors.forename: Yuyun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No.11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No.11 BeiYiTiao, ZhongGuanCun, Beijing, 100190, P. R. China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000473:pathology*; D035843:Biomedical Research; D060833:Cellular Microenvironment; D006439:Hemodynamics; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D057446:Hydrodynamics; D006943:Hyperglycemia / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D051379:Mice; D044085:Microfluidics / Q000379:methods*; D008954:Models, Biological; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species
keywords: atherosclerosis; blood vessels; drug screening; mechanics; microfluidic chips