title: A fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters.
abstract: A fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer-templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA-AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as-prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA-AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran. smhosseini@khayam.ut.ac.ir.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mehrabi
authors.affiliation: Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran.|Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ganjali
authors.affiliation: Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran.|Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Norouzi
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000592:standards; D005453:Fluorescence; D006722:Honey / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010118:Oxytetracycline / Q000032:analysis* / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D014867:Water; D012834:Silver; D010118:Oxytetracycline
keywords: aptamer; fluorescence; nanobiosensor; oxytetracycline; quenching; silver nanoclusters