title: Bisphenol a influences blastocyst implantation via regulating integrin β3 and trophinin expression levels.
abstract: OBJECTIVE
  This study is to investigate effects of bisphenol A (BPA) on the blastocyst implantation in endometrium.
  
  
  METHODS
  Pregnant mice were orally administered with BPA. Implantation sites were examined, and serum estrogen level was assayed with ELISA. Protein expression levels were detected with immunohistochemistry and immunofluorescence staining.
  
  
  RESULTS
  High doses (400 and 600 mg/kg/day) of BPA remarkably reduced the implantation sites in the pregnant mice. No significant differences were observed in the serum estrogen level across the groups. Moreover, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression level of endometrial estrogen receptor α (ERα) in the pregnant mice. In addition, high doses (400 and 600 mg/kg/day) of BPA significantly declined the expression levels of integrin β3 and trophinin in the endometrium and blastocysts.
  
  
  CONCLUSIONS
  BPA declines ERα expression in endometrium, and inhibits adhesion protein expression in endometrium and blastocysts, causing the adhesion failure of blastocyst implantation.
authors.affiliation: Department of Histology and Embryology, Jilin Medical University Jilin 132013, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Histology and Embryology, Jilin Medical University Jilin 132013, China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Provincial Key Laboratory for Developmental Biology and Neuroscience, College of Life Science, Fujian Normal University Fuzhou 350108, Fujian, China.
authors.forename: Zhengchao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Reproductive Medicine Center, Affiliated Hospital of Jining Medical College Jining 272029, Shandong, China.
authors.forename: Xuenan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Jilin Medical University Jilin 132013, China.
authors.forename: Zhaohua
authors.initials: Z
authors.lastname: Dou
authors.affiliation: Department of Histology and Embryology, Jilin Medical University Jilin 132013, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Li
keywords: Blastocyst implantation; bisphenol A (BPA); estrogen receptor; integrin β3; trophinin