title: A new bioenergetic and thermodynamic approach to batch photoautotrophic growth of Arthrospira (Spirulina) platensis in different photobioreactors and under different light conditions.
abstract: Photobioreactor configuration, mode of operation and light intensity are known to strongly impact on cyanobacteria growth. To shed light on these issues, kinetic, bioenergetic and thermodynamic parameters of batch Arthrospira platensis cultures were estimated along the time at photosynthetic photon flux density (PPFD) of 70μmolm(-2)s(-1) in different photobioreactors with different surface/volume ratio (S/V), namely open pond (0.25cm(-1)), shaken flask (0.48cm(-1)), horizontal photobioreactor (HoP) (1.94cm(-1)) and helicoidal photobioreactor (HeP) (3.88cm(-1)). Maximum biomass concentration and productivity remarkably increased with S/V up to 1.94cm(-1). HoP was shown to be the best-performing system throughout the whole runs, while HeP behaved better only at the start. Runs carried out in HoP increasing PPFD from 40 to 100μmolm(-2)s(-1) revealed a progressive enhancement of bioenergetics and thermodynamics likely because of favorable light distribution. HoP appeared to be a promising configuration to perform high-yield indoor cyanobacterial cultures.
authors.affiliation: Biological Science Center, Federal University of Pernambuco, Av. Prof. Moraes Rego 1235, Cidade Universitária, 50670-420 Recife, PE, Brazil; Department of Civil, Chemical and Environmental Engineering (DICCA), University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy.
authors.forename: Milena Fernandes
authors.initials: MF
authors.lastname: da Silva
authors.affiliation: Department of Civil, Chemical and Environmental Engineering (DICCA), University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy.
authors.forename: Alessandro Alberto
authors.initials: AA
authors.lastname: Casazza
authors.affiliation: Department of Civil, Chemical and Environmental Engineering (DICCA), University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy.
authors.forename: Pier Francesco
authors.initials: PF
authors.lastname: Ferrari
authors.affiliation: Department of Civil, Chemical and Environmental Engineering (DICCA), University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Perego
authors.affiliation: Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900 Recife, PE, Brazil.
authors.forename: Raquel Pedrosa
authors.initials: RP
authors.lastname: Bezerra
authors.affiliation: Department of Civil, Chemical and Environmental Engineering (DICCA), University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy. Electronic address: converti@unige.it.
authors.forename: Attilio
authors.initials: A
authors.lastname: Converti
authors.affiliation: Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s/n, 52171-900 Recife, PE, Brazil.
authors.forename: Ana Lucia Figueiredo
authors.initials: AL
authors.lastname: Porto
mesh_terms: D052818:Autotrophic Processes / Q000528:radiation effects*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D004734:Energy Metabolism / Q000528:radiation effects; D008027:Light; D058433:Photobioreactors / Q000382:microbiology*; D017785:Photons; D053545:Spirulina / Q000254:growth & development* / Q000528:radiation effects*; D013816:Thermodynamics; D013997:Time Factors
keywords: Arthrospira platensis; Bioenergetics; Light intensity; Photobioreactor; Thermodynamics