title: Glycomics and glycoproteomics of membrane proteins and cell-surface receptors: Present trends and future opportunities.
abstract: Membrane proteins mediate cell-cell interactions and adhesion, the transfer of ions and metabolites, and the transmission of signals from the extracellular environment to the cell interior. The extracellular domains of most cell membrane proteins are glycosylated, often at multiple sites. There is a growing awareness that glycosylation impacts the structure, interaction, and function of membrane proteins. The application of glycoproteomics and glycomics methods to membrane proteins has great potential. However, challenges also arise from the unique physical properties of membrane proteins. Successful analytical workflows must be developed and disseminated to advance functional glycoproteomics and glycomics studies of membrane proteins. This review explores the opportunities and challenges related to glycomic and glycoproteomic analysis of membrane proteins, including discussion of sample preparation, enrichment, and MS/MS analyses, with a focus on recent successful workflows for analysis of N- and O-linked glycosylation of mammalian membrane proteins.
authors.affiliation: Center for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Kevin Brown
authors.initials: KB
authors.lastname: Chandler
authors.affiliation: Center for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
mesh_terms: D000818:Animals; D054794:Glycomics / Q000639:trends*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis; D040901:Proteomics / Q000639:trends*; D011956:Receptors, Cell Surface / Q000032:analysis; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins; D011956:Receptors, Cell Surface
keywords: Glycomics; Glycopeptides; Glycoproteomics; Membrane proteins; Receptors