title: The Critical Role of SRPK1 in EMT of Human Glioblastoma in the Spinal Cord.
abstract: Up to now, the serine-arginine protein kinase 1 (SRPK1) has been suggested as an important signal mediator, which is implicated in the development of cancers. Unfortunately, some molecular pathways in SRPK1-mediated epithelial-mesenchymal transition (EMT) in human spinal glioblastoma have been not elucidated. In this work, we detected the expression of SRPK1 in human spinal glioblastoma tissues and GBM cell lines and analyzed the relevant molecular proteins using in vitro experiments, including RT-PCR, gene silencing, and Western blot. In this study, RT-PCR and Western blot revealed that the expression of SRPK1 mRNA and protein became higher in all six spinal glioblastoma specimens; however, its expression was low in matched normal specimens. We also demonstrated SRPK1 expression facilitated the proliferation of U87 and U251 cells and inhibited the apoptosis in U87 and U251 cells. Also, SRPK1 promoted the expression of EMT-regulating markers, involving N-cadherin, Snail, and MMP9 and decreased the expression of mesenchymal marker E-cadherin. Moreover, knockdown of SRPK1 significantly inhibited the expression levels of p-Akt rather than t-Akt. In conclusion, knockdown of SRPK1 inhibited glioblastoma cell proliferation, invasion, and EMT process via suppressing p-Akt signaling pathway. This study also lays a new foundation for the clinically biological treatment.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, ChongQing, 400016, China. liaozhengbu163@163.com.
authors.forename: Zhengbu
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Intensive Care Unit, The Second Affiliated Hospital of Chongqing Medical University, ChongQing, 400010, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Molecular Biology, Chongqing Medical University, ChongQing, 400016, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, ChongQing, 400016, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, ChongQing, 400016, China.
authors.forename: Haiquan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, ChongQing, 400016, China.
authors.forename: Chongjie
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, ChongQing, 400016, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Tang
keywords: EMT; Glioblastoma; SRPK1