title: The Global AIMs Nano set: A 31-plex SNaPshot assay of ancestry-informative SNPs.
abstract: A 31-plex SNaPshot assay, named 'Global AIMs Nano', has been developed by reassembling the most differentiated markers of the EUROFORGEN Global AIM-SNP set. The SNPs include three tri-allelic loci and were selected with the goal of maintaining a balanced differentiation of: Africans, Europeans, East Asians, Oceanians and Native Americans. The Global AIMs Nano SNP set provides higher divergence between each of the five continental population groups than previous small-scale AIM sets developed for forensic ancestry analysis with SNaPshot. Both of these characteristics minimise potential bias when estimating co-ancestry proportions in individuals with admixed ancestry; more likely to be observed when using markers disproportionately informative for only certain population group comparisons. The optimised multiplex is designed to be easily implemented using standard capillary electrophoresis regimes and has been used to successfully genotype challenging forensic samples from highly degraded material with low level DNA. The ancestry predictive performance of the Global AIMs Nano set has been evaluated by the analysis of samples previously characterised with larger AIM sets.
authors.affiliation: Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain.
authors.forename: M
authors.initials: M
authors.lastname: de la Puente
authors.affiliation: Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Santos
authors.affiliation: Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Fondevila
authors.affiliation: Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Manzo
authors.affiliation: Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain; Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Á
authors.initials: Á
authors.lastname: Carracedo
authors.affiliation: Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain.
authors.forename: M V
authors.initials: MV
authors.lastname: Lareu
authors.affiliation: Forensic Genetics Unit, Institute of Forensic Sciences, University of Santiago de Compostela, Spain. Electronic address: c.phillips@mac.com.
authors.forename: C
authors.initials: C
authors.lastname: Phillips
mesh_terms: D004247:DNA / Q000235:genetics; D053803:Forensic Genetics / Q000379:methods* / Q000592:standards; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population / Q000379:methods; D005838:Genotype; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D020641:Polymorphism, Single Nucleotide; D044469:Racial Groups / Q000235:genetics*
chemical_list: D004247:DNA
keywords: AIMs; Biogeographical ancestry; Population-specific Divergence; SNPs; SNaPshot