title: Changes in Predominance of Pulsed-Field Gel Electrophoresis Profiles of Bordetella pertussis Isolates, United States, 2000-2012.
abstract: To clarify the characteristics of circulating Bordetella pertussis isolates, we used pulsed-field gel electrophoresis (PFGE) to analyze 5,262 isolates collected in the United States during 2000-2012. We found 199 PFGE profiles; 5 profiles accounted for 72% of isolates. The most common profile, CDC013, accounted for 35%-46% of isolates tested from 2000-2009; however, the proportion of isolates of this profile rapidly decreased in 2010. Profile CDC237, first seen in 2009, increased rapidly and accounted for 29% of 2012 isolates. No location bias was observed among profiles during 2000-2010, but differences were observed among isolates from different states during 2012. Predominant profiles match those observed in recent European PFGE studies. PFGE profile changes are concurrent with other recent molecular changes in B. pertussis and may be contributing to the reemergence of pertussis in the United States. Continued PFGE monitoring is critical for understanding the changing epidemiology of pertussis.
authors.forename: Pamela K
authors.initials: PK
authors.lastname: Cassiday
authors.forename: Tami H
authors.initials: TH
authors.lastname: Skoff
authors.forename: Selina
authors.initials: S
authors.lastname: Jawahir
authors.forename: M Lucia
authors.initials: ML
authors.lastname: Tondella
mesh_terms: D001886:Bordetella pertussis / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D006801:Humans; D014481:United States; D014917:Whooping Cough / Q000382:microbiology
keywords: Bordetella pertussis; United States; bacteria; pertactin; pertussis; pulsed-field gel electrophoresis; respiratory infections; whooping cough