title: Potential role of autophagy in smokeless tobacco extract-induced cytotoxicity and in morin-induced protection in oral epithelial cells.
abstract: Toxic components of STE induced serious, adverse human oral health outcomes. In the present study, we observed that STE was involved in oral toxicity by reducing the viability of human squamous epithelial cells, SCC-25, along with the simultaneous induction of both apoptosis and autophagic signaling. STE was also found to induce significant amount ROS generation in SCC-25 cells. The dietary flavonoid morin, found abundantly in a variety of herbs, fruits and wine, has been reported to attenuate ROS-induced pathogenesis including autophagy. In this study we designed three different treatment regimes of morin treatment, such as pre, co, and post - treatment of STE challenged SCC-25 cells. In all cases morin provided cytoprotection to STE challenged SCC-25 cells by augmenting STE induced ROS-dependent cytotoxic autophagy. Hence, morin is a potential option for antioxidant therapy in treatment of STE induced toxicity.
authors.affiliation: Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB, 700019, India.
authors.forename: Arnab
authors.initials: A
authors.lastname: Ganguli
authors.affiliation: Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB, 700019, India.
authors.forename: Amlan
authors.initials: A
authors.lastname: Das
authors.affiliation: Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB, 700019, India.
authors.forename: Debasish
authors.initials: D
authors.lastname: Nag
authors.affiliation: Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB, 700019, India.
authors.forename: Surela
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB, 700019, India. Electronic address: gcbcg@caluniv.ac.in.
authors.forename: Gopal
authors.initials: G
authors.lastname: Chakrabarti
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000187:drug effects*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D009062:Mouth Neoplasms; D014030:Tobacco, Smokeless / Q000633:toxicity*; D047309:Flavones
chemical_list: D005419:Flavonoids; C008548:morin; D047309:Flavones
keywords: Autophagy; Morin; Reactive oxygen species; Smokeless tobacco