title: Usefulness of a bioengineered oral mucosa model for preventing palate bone alterations in rabbits with a mucoperiostial defect.
abstract: The use of mucoperiostial flaps during cleft palate surgery is associated with altered palatal bone growth and development. We analyzed the potential usefulness of a bioengineered oral mucosa in an in vivo model of cleft palate. First, a 4 mm palate defect was created in one side of the palate oral mucosa of 3 week-old New Zealand rabbits, and a complete autologous bioengineered oral mucosa (BOM) or acellular fibrin-agarose scaffold (AS) was implanted. No material was implanted in the negative controls (NC), and positive controls were not subjected to palatal defect (PC). Animals were allowed to grow for 6 months and the results were analyzed morphologically (palate mucosa and bone size) and histologically. Results show that palatal mucosa and bone growth and development were significantly altered in NC and AS animals, whereas BOM animals had similar results to PC and the bioengineered oral mucosa was properly integrated in the host palate. The amount and compaction of collagen fibers was similar between BOM and PC, and both groups of animals had comparable contents of proteoglycans and glycoproteins at the palate bone. No differences were found for decorin, osteocalcin and BMP2. The use of bioengineered oral mucosa substitutes is able to improve palate growth and maturation by preventing the alterations found in animals with denuded palate bone. These results support the potential clinical usefulness of BOM substitutes for the treatment of patients with cleft palate and other conditions in which palate mucosa grafts are necessary with consequent bone denudation.
authors.affiliation: Division of Oral and Maxillofacial Surgery, University General Hospital Gregorio Marañón, Madrid, Spain. PhD programme in Biomedicine, University of Granada, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernández-Valadés-Gámez
authors.forename: Ingrid
authors.initials: I
authors.lastname: Garzón
authors.forename: Esther
authors.initials: E
authors.lastname: Liceras-Liceras
authors.forename: Antonio
authors.initials: A
authors.lastname: España-López
authors.forename: Víctor
authors.initials: V
authors.lastname: Carriel
authors.forename: Miguel-Ángel
authors.initials: MÁ
authors.lastname: Martin-Piedra
authors.forename: María-Ángeles
authors.initials: MÁ
authors.lastname: Muñoz-Miguelsanz
authors.forename: Maria-Carmen
authors.initials: MC
authors.lastname: Sánchez-Quevedo
authors.forename: Miguel
authors.initials: M
authors.lastname: Alaminos
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernández-Valadés
mesh_terms: D000818:Animals; D021522:Bioartificial Organs; D040761:Biomimetic Materials / Q000627:therapeutic use*; D002972:Cleft Palate / Q000473:pathology / Q000628:therapy*; D005337:Fibrin / Q000627:therapeutic use*; D008422:Materials Testing; D009061:Mouth Mucosa / Q000737:chemistry* / Q000637:transplantation; D021362:Palate, Hard / Q000473:pathology; D011817:Rabbits; D012685:Sepharose / Q000627:therapeutic use*; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D005337:Fibrin; D012685:Sepharose