title: Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry.
abstract: OBJECTIVE
  The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA).
  
  
  METHODS
  Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP).
  
  
  RESULTS
  On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP.
  
  
  CONCLUSIONS
  Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
authors.forename: Jia
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Song
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Jeonju, Korea.
authors.forename: Kyung-San
authors.initials: KS
authors.lastname: Min
authors.affiliation: Research Center for Biomineralization Disorders, Chonnam National University, Gwangju, Korea.
authors.forename: Sun-Hun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Research Center for Biomineralization Disorders, Chonnam National University, Gwangju, Korea.
authors.forename: Jeong-Tae
authors.initials: JT
authors.lastname: Koh
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
authors.forename: Bin-Na
authors.identifier: 0000-0001-8017-1835
authors.initials: BN
authors.lastname: Lee
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
authors.forename: Hoon-Sang
authors.identifier: 0000-0002-3019-1528
authors.initials: HS
authors.lastname: Chang
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
authors.forename: In-Nam
authors.identifier: 0000-0002-5388-1919
authors.initials: IN
authors.lastname: Hwang
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
authors.forename: Won-Mann
authors.identifier: 0000-0001-6480-6191
authors.initials: WM
authors.lastname: Oh
authors.affiliation: Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.; Research Center for Biomineralization Disorders, Chonnam National University, Gwangju, Korea.
authors.forename: Yun-Chan
authors.initials: YC
authors.lastname: Hwang
keywords: BioAggregate; Biodentine; Dentin sialoprotein; MTA; Micro-CT