title: Organogenesis and Ultrastructural Features of In Vitro Grown Canna indica L.
abstract: An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces. Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sharifah Nurashikin
authors.initials: SN
authors.lastname: Wafa
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Rosna
authors.initials: R
authors.lastname: Mat Taha
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sadegh
authors.initials: S
authors.lastname: Mohajer
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Noraini
authors.initials: N
authors.lastname: Mahmad
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Bakrudeen
authors.initials: B
authors.lastname: Ali Ahmed Abdul
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D038081:Organogenesis; D018515:Plant Leaves / Q000254:growth & development / Q000648:ultrastructure; D018517:Plant Roots / Q000254:growth & development / Q000648:ultrastructure; D018520:Plant Shoots / Q000254:growth & development / Q000648:ultrastructure; D010946:Plants, Medicinal / Q000254:growth & development* / Q000648:ultrastructure; D036226:Seedlings / Q000254:growth & development / Q000648:ultrastructure; D019862:Zingiberales / Q000254:growth & development* / Q000648:ultrastructure