title: The Rise of CRISPR/Cas for Genome Editing in Stem Cells.
abstract: Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models. Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology.
authors.affiliation: Department of Biology, Carleton College, Northfield, MN 55057, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Shui
authors.affiliation: Department of Biology, University of Puerto Rico, Rio Piedras, San Juan, PR 00931, USA.
authors.forename: Liz
authors.initials: L
authors.lastname: Hernandez Matias
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA; Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng