title: USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells.
abstract: OBJECTIVE
  This study aimed to explore the influence of USO1 on multiple myeloma (MM) cell proliferation and apoptosis and the related molecular mechanism.
  
  
  METHODS
  The expression of USO1 and MIF in MM tissues and cells, normal bone marrow tissues and cells were determined by qRT-PCR and western blot assay. The cell proliferation and apoptosis of MM cells before and after knockdown of USO1 were determined by MTT assay and flow cytometry, respectively. Before and after knockdown of USO1, the expression of the proliferation-related genes cyclin D1, Mcm2 and PCNA in MM cells was determined by qRT-PCR and western blot assay. The protein level of p-Erk1/2 and MIF was determined by western blot assay and ELISA, respectively.
  
  
  RESULTS
  The expression levels of USO1 and MIF in MM tissues and cells were much higher than those in normal bone marrow tissues and cells. Knockdown of USO1 resulted in the inhibited ability of cell proliferation and induced cell apoptosis. The expression of cyclin D1, Mcm2, PCNA and p-Erk1/2 decreased significantly after knockdown of USO1 as well as the decreased MIF secretion.
  
  
  CONCLUSIONS
  USO1 gene may be a promising target for the therapy of human MM and its diagnosis marker.
authors.affiliation: Department of Pathology, Shanghai University of Medicine and Health Sciences, Shanghai 200237, China.
authors.forename: Yueling
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Hematology, Shanghai Pudong Hospital Affiliated to Fudan University, Shanghai 201399, China. Electronic address: zhenshengdai@126.com.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Dai
mesh_terms: D017209:Apoptosis; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D018450:Disease Progression; D015536:Down-Regulation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D000077614:Golgi Matrix Proteins; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008263:Macrophage Migration-Inhibitory Factors / Q000378:metabolism; D009101:Multiple Myeloma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D000077614:Golgi Matrix Proteins; D008263:Macrophage Migration-Inhibitory Factors; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins; C091580:vesicular transport factor p115; D048049:Extracellular Signal-Regulated MAP Kinases; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human
keywords: Cell proliferation; MIF; Multiple myeloma; USO1; p-Erk1/2