title: A Rapid Study of Botanical Drug-Drug Interaction with Protein by Re-ligand Fishing using Human Serum Albumin-Functionalized Magnetic Nanoparticles.
abstract: A great many active constituents of botanical drugs bind to human serum albumin (HSA) reversibly with a dynamic balance between the free- and bound-forms in blood. The curative or side effect of a drug depends on its free-form level, which is always influenced by other drugs, combined dosed or multi-constituents of botanical drugs. This paper presented a rapid and convenient methodology to investigate the drug-drug interactions with HSA. The interaction of two steroidal saponins, dioscin and pseudo-protodioscin, from a botanical drug was studied for their equilibrium time and equilibrium amount by re-ligand fishing using HSA functionalized magnetic nanoparticles. A clear competitive situation was obtained by this method. The equilibrium was reached soon about 15 s at a ratio of 0.44: 1. Furthermore, the interaction of pseudo-protodioscin to total steroidal saponins from DAXXK was also studied. The operation procedures of this method were faster and more convenient compared with other methods reported.
authors.forename: Lin-Sen
authors.initials: LS
authors.lastname: Qing
authors.forename: Ying
authors.initials: Y
authors.lastname: Xue
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Ding
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Liang
authors.forename: Xun
authors.initials: X
authors.lastname: Liao
mesh_terms: D004144:Diosgenin / Q000031:analogs & derivatives / Q000737:chemistry; D004347:Drug Interactions; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*
chemical_list: D012709:Serum Albumin; C000606215:pseudo-protodioscin; C019357:dioscin; D004144:Diosgenin