title: [FLUORESCENCE DISTRIBUTION IN BONE AND CARTILAGE TISSUE BY SECTIONING OF FROZEN UNDECALCIFIED BONE].
abstract: OBJECTIVE
  To introduce a technique of frozen sections for undecalcified bone and discuss its feasiblity by observing the fluorescence distribution of the bone and cartilage.
  
  
  METHODS
  The male Sprague Dawley transgenic rats at the age of 8 weeks, which express green fluoreschent protein were selected to isolate the whole knee sectioned by teh undecalicified bone fronze section techynique. Under the fluorescence and light microscopy, the fluorescence and structure were observed within the organization of slice. Immunohistochemical staining (collagen type I and II, He staining, toluidine blue staining, and Alizarin red staining were performed to observe the distribution of fluorescent substance and cartilage and bone structure.
  
  
  RESULTS
  The thickness of sections prepared by this technology was 6 µm. General observation showed that the structure of secioned joint was complete. Under the light microscope, the morphology of cartilage cells, the arrangement of subchondral bone, and trabecular bone traveling could be clearly distinguished. Under fluorescence microscope, green fluorescence was shonw in the joint soft tissue, cartilage tissue, and bone tissue; collagen type I expressed in the bone tissue, collage type II in cartilage tissue. HE staining and toluidine blue staining could clearly distinguish the morphology of the cartilage layer. Alizarin red staining showed the structural integrity of subchondral bone plate and the organization within the meniscus, and proximal tibia cortical bone continuity.
  
  
  CONCLUSIONS
  The fluorescence distribution can be directly observe in the bone and cartilage by sectioning of frozen undecalcified bone. This new technology can shorten the cycle of preparing sections.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.forename: Heyong
authors.initials: H
authors.lastname: Yin
authors.forename: Xiang
authors.initials: X
authors.lastname: Sui
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yu
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Aiyuan
authors.initials: A
authors.lastname: Wang
authors.forename: Quanyi
authors.initials: Q
authors.lastname: Guo
authors.lastname: Liu Shuyun
authors.forename: Haoye
authors.initials: H
authors.lastname: Meng
authors.forename: Shibi
authors.initials: S
authors.lastname: Lu
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002358:Cartilage, Articular; D024042:Collagen Type I; D005453:Fluorescence; D007719:Knee Joint; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia
chemical_list: D024042:Collagen Type I