title: Extraction of high-quality RNA from pancreatic tissues for gene expression studies.
abstract: Extracting RNA from pancreatic tissue is notoriously challenging because of the organ's high RNase content. Standard methods using TriPure or TRIzol classically yield RNA of sufficient quality for routine gene expression analysis but not for microarray or deep sequencing analysis. Here we developed a simple method to extract high-quality RNA from mouse pancreas. Our method uses an RNase inhibitor and combines different protocols using guanidium thiocyanate-phenol extraction. It enables reproducible isolation of RNA with an RNA integrity number around 9.
authors.affiliation: de Duve Institute, Université catholique de Louvain, B-1200 Brussels, Belgium.
authors.forename: Cécile
authors.initials: C
authors.lastname: Augereau
authors.affiliation: de Duve Institute, Université catholique de Louvain, B-1200 Brussels, Belgium.
authors.forename: Frédéric P
authors.initials: FP
authors.lastname: Lemaigre
authors.affiliation: de Duve Institute, Université catholique de Louvain, B-1200 Brussels, Belgium. Electronic address: patrick.jacquemin@uclouvain.be.
authors.forename: Patrick
authors.initials: P
authors.lastname: Jacquemin
mesh_terms: D000818:Animals; D006801:Humans; D051379:Mice; D010179:Pancreas / Q000737:chemistry*; D012334:RNA, Neoplasm / Q000302:isolation & purification*
chemical_list: D012334:RNA, Neoplasm
keywords: Mouse; Pancreas; RNA; RNA integrity number; RNA purification