title: Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10.
abstract: Enzyme production by Aspergillus terreus NCFT 4269.10 was studied under liquid static surface and solid-state fermentation using mustard oil cake as a substrate. The maximum lipase biosynthesis was observed after incubation at 30°C for 96h. Among the domestic oils tested, the maximum lipase biosynthesis was achieved using palm oil. The crude lipase was purified 2.56-fold to electrophoretic homogeneity, with a yield of 8.44%, and the protein had a molecular weight of 46.3kDa as determined by SDS-PAGE. Enzyme characterization confirmed that the purified lipase was most active at pH 6.0, temperature of 50°C, and substrate concentration of 1.5%. The enzyme was thermostable at 60°C for 1h, and the optimum enzyme-substrate reaction time was 30min. Sodium dodecyl sulfate and commercial detergents did not significantly affect lipase activity during 30-min incubation at 30°C. Among the metal ions tested, the maximum lipase activity was attained in the presence of Zn(2+), followed by Mg(2+) and Fe(2+). Lipase activity was not significantly affected in the presence of ethylenediaminetetraacetic acid, sodium lauryl sulfate and Triton X-100. Phenylmethylsulfonyl fluoride (1mM) and the reducing, β-mercaptoethanol significantly inhibited lipase activity. The remarkable stability in the presence of detergents, additives, inhibitors and metal ions makes this lipase unique and a potential candidate for significant biotechnological exploitation.
authors.affiliation: Microbiology Research Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar 751004, Odisha, India; MITS School of Biotechnology, 2 (P), Infocity, Patia, Chandaka Industrial Estate, Bhubaneswar 751024, Odisha, India. Electronic address: reach4bijay@gmail.com.
authors.forename: Bijay Kumar
authors.initials: BK
authors.lastname: Sethi
authors.affiliation: Department of Botany, Saila Bala Women's College, Cuttack 753001, Odisha, India.
authors.forename: Prativa Kumari
authors.initials: PK
authors.lastname: Nanda
authors.affiliation: Microbiology Research Laboratory, P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar 751004, Odisha, India.
authors.forename: Santilata
authors.initials: S
authors.lastname: Sahoo
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002413:Cations, Divalent / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D020536:Enzyme Activators / Q000032:analysis; D004791:Enzyme Inhibitors / Q000032:analysis; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008623:Mercaptoethanol / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D008970:Molecular Weight; D013696:Temperature
chemical_list: D002413:Cations, Divalent; D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D008670:Metals; D008623:Mercaptoethanol; D008049:Lipase
keywords: Aspergillus terreus; Lipase; Liquid static surface fermentation; Solid-state fermentation