title: Effect of Human Serum and 2 Different Types of Platelet Concentrates on Human Meniscus Cell Migration, Proliferation, and Matrix Formation.
abstract: To evaluate the effect of 10% human serum (HS), 5% platelet-rich plasma (PRP), and 5% autologous conditioned plasma (ACP) on migration, proliferation, and extracellular matrix (ECM) synthesis of human meniscus cells.
  
  
  
  Cell migration and proliferation on stimulation with HS, PRP, and ACP were assessed by chemotaxis assays and measurement of genomic DNA content. Meniscus cells were cultivated in pellets stimulated with 10% HS, 5% PRP, or 5% ACP. Meniscal ECM formation was evaluated by histochemical staining of collagen type I, type II, and proteoglycans and by analysis of fibrochondrocyte marker gene expression.
  
  
  
  Human meniscus cells were significantly attracted by all 3 blood-derived products (10% HS and 5% ACP: P = .0001, 5% PRP: P = .0002). Cell proliferation at day 9 was significantly increased on stimulation with 10% HS (P = .0001) and 5% PRP (P = .0002) compared with 5% ACP and controls. Meniscus cell pellet cultures showed the formation of a well-structured meniscal ECM with deposition of collagen type I, type II, and proteoglycans on stimulation with 10% HS, whereas 5% PRP or 5% ACP resulted in the formation of an inhomogeneous and more fibrous ECM. Stimulation with 10% HS and 5% ACP showed a significant induction of fibrochondrocyte marker genes such as aggrecan (HS: P = .0002, ACP: P = .0147), cartilage oligomeric matrix protein (HS: P = .0002, ACP: P = .0005), and biglycan (HS: P = .0002, ACP: P = .0003), whereas PRP showed no inducing effect.
  
  
  
  Among all tested blood-derived products, only stimulation with HS showed the formation of a meniscal ECM as well as positive cell proliferating and migrating effects in vitro. Regarding a potential biological repair of nonvascular meniscus lesions, our results may point toward the use of HS as a beneficial augment in regenerative meniscus repair approaches.
  
  
  
  Our findings may suggest that HS might be a beneficial augment for meniscus repair.
authors.affiliation: TransTissue Technologies GmbH, Department of Research & Development, Berlin, Germany. Electronic address: undine.freymann@transtissue.com.
authors.forename: Undine
authors.initials: U
authors.lastname: Freymann
authors.affiliation: Clinic for Traumatic Surgery and Orthopedics, Martin-Luther-Hospital, Berlin, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Metzlaff
authors.affiliation: TransTissue Technologies GmbH, Department of Research & Development, Berlin, Germany.
authors.forename: Jan-Philipp
authors.initials: JP
authors.lastname: Krüger
authors.affiliation: TransTissue Technologies GmbH, Department of Research & Development, Berlin, Germany; DeSimone Laboratory, Department of Cell Biology, University of Virginia, Charlottesville, Virginia, U.S.A.
authors.forename: Glen
authors.initials: G
authors.lastname: Hirsh
authors.affiliation: TransTissue Technologies GmbH, Department of Research & Development, Berlin, Germany; Tissue Engineering Laboratory, Department of Rheumatology and Immunology, Charité - University Hospital Berlin, Berlin, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Endres
authors.affiliation: Clinic for Traumatic Surgery and Orthopedics, Martin-Luther-Hospital, Berlin, Germany.
authors.forename: Wolf
authors.initials: W
authors.lastname: Petersen
authors.affiliation: TransTissue Technologies GmbH, Department of Research & Development, Berlin, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Kaps
mesh_terms: D000368:Aged; D001792:Blood Platelets / Q000502:physiology*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002633:Chemotaxis; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008592:Menisci, Tibial / Q000166:cytology* / Q000378:metabolism; D000072600:Meniscus; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism; D044967:Serum / Q000502:physiology*
chemical_list: D024042:Collagen Type I; D024043:Collagen Type II; D011509:Proteoglycans