title: A facile and efficient method of enzyme immobilization on silica particles via Michael acceptor film coatings: immobilized catalase in a plug flow reactor.
abstract: A novel method was developed for facile immobilization of enzymes on silica surfaces. Herein, we describe a single-step strategy for generating of reactive double bonds capable of Michael addition on the surfaces of silica particles. This method was based on reactive thin film generation on the surfaces by heating of impregnated self-curable polymer, alpha-morpholine substituted poly(vinyl methyl ketone) p(VMK). The generated double bonds were demonstrated to be an efficient way for rapid incorporation of enzymes via Michael addition. Catalase was used as model enzyme in order to test the effect of immobilization methodology by the reactive film surface through Michael addition reaction. Finally, a plug flow type immobilized enzyme reactor was employed to estimate decomposition rate of hydrogen peroxide. The highly stable enzyme reactor could operate continuously for 120 h at 30 °C with only a loss of about 36 % of its initial activity.
authors.affiliation: Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500, Ankara, Turkey. g_bayramoglu@hotmail.com.|Department of Chemistry, Faculty of Sciences, Gazi University, 06500, Ankara, Turkey. g_bayramoglu@hotmail.com.
authors.forename: Gulay
authors.initials: G
authors.lastname: Bayramoglu
authors.affiliation: Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500, Ankara, Turkey.
authors.forename: M Yakup
authors.initials: MY
authors.lastname: Arica
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University, 06500, Ankara, Turkey.
authors.forename: Aysenur
authors.initials: A
authors.lastname: Genc
authors.affiliation: School of Medicine, Istanbul Kemerburgaz University, 34217, Istanbul, Turkey.
authors.forename: V Cengiz
authors.initials: VC
authors.lastname: Ozalp
authors.affiliation: Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ince
authors.affiliation: Department of Chemistry, Istanbul Technical University, Maslak, 34469, Istanbul, Turkey.
authors.forename: Niyazi
authors.initials: N
authors.lastname: Bicak
mesh_terms: D019149:Bioreactors; D002374:Catalase / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D002374:Catalase
keywords: Catalase; Enzyme bioreactors; Immobilized enzymes; Michael addition; Surface modification