title: Urinary gamma-glutamyl transferase (GGT) as a marker of tubular proteinuria in dogs with canine leishmaniasis, using sodium dodecylsulphate (SDS) electrophoresis as a reference method.
abstract: In order to assess if urinary γ- glutamyl transferase (GGT) identify tubular proteinuria in leishmaniotic dogs, the GGT/urinary creatinine (UC) ratio was calculated in 39 leishmaniotic dogs. According to sodium dodecylsulphate-agarose gel electrophoresis, the dogs had albuminuria (A, n = 10), glomerular (G, n = 3), tubular (T, n = 4) or mixed proteinuria (M, n = 22). The median GGT/UC ratio was 0.3, 0.3, 2.2, and 7.5, in groups G, A, M, and T, respectively. Statistically significant differences were found between groups G and M (P = 0.002), G and T (P <0.001), A and M (P <0.001), and A and T (P <0.001). Median values were higher in dogs with tubular components of proteinuria (M/T, 2.5) than in dogs without tubular components of proteinuria (A/G, 0.3), and in dogs with tubular proteinuria (T, 7.5) than in dogs with non-tubular proteinuria (NT, 1.0). GGT/UC values >0.81 or >2.64 could identify dogs in the M/T or T groups, respectively. Therefore, GGT/UC might be useful for the management of leishmaniotic dogs.
authors.affiliation: Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy; Private laboratory 'Poggio dei Pini', Centro Commerciale Strada 40, 09012 Capoterra (CA), Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ibba
authors.affiliation: Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mangiagalli
authors.affiliation: Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy. Electronic address: saverio.paltrinieri@unimi.it.
authors.forename: S
authors.initials: S
authors.lastname: Paltrinieri
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D007674:Kidney Diseases / Q000150:complications / Q000652:urine / Q000662:veterinary; D007684:Kidney Tubules / Q000473:pathology; D007896:Leishmaniasis / Q000150:complications / Q000473:pathology / Q000652:urine / Q000662:veterinary*; D008297:Male; D011507:Proteinuria / Q000652:urine / Q000662:veterinary*; D012015:Reference Standards; D005723:gamma-Glutamyltransferase / Q000652:urine*
chemical_list: D015415:Biomarkers; D005723:gamma-Glutamyltransferase
keywords: Canine leishmaniasis; Proteinuria; Renal tubular damage; Sodium dodecylsulphate electrophoresis (SDS); Urinary GGT