title: pH sensitive coiled coils: a strategy for enhanced liposomal drug delivery.
abstract: Stimuli responsive controlled release from liposome based vesicles is a promising strategy for the site specific delivery of drugs. Herein, we report the design of pH sensitive coiled coils and their incorporation into the liposome as triggers for the controlled release of encapsulated drugs. The designed coiled coil peptides with the incorporation of environment sensitive fluorescent amino acids were found to be stable at physiological pH and unstructured while changing the pH of the environment to either acidic or basic. This pH dependent conformational switch of the coiled-coil polypeptides was exploited as triggers for the enhanced release of the encapsulated drug molecules from liposomes. The SEM, DLS and TEM analysis revealed the uniform morphology of the peptide liposome hybrid vesicles. Further, the drug encapsulated liposome internalization experiments with cancer cells revealed the enhanced release and accumulation of drugs in the acidic lysosomal compartments in comparison with liposomes without coiled coils.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research, Dr Homi Bhabha Road, Pune-411021, India. hn.gopi@iiserpune.ac.in.
authors.forename: Rahi M
authors.initials: RM
authors.lastname: Reja
authors.affiliation: National Center for Cell Sciences, Pune University Campus, Pune-411 007, India. anjali@nccs.res.in.
authors.forename: Mohsina
authors.initials: M
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research, Dr Homi Bhabha Road, Pune-411021, India. hn.gopi@iiserpune.ac.in.
authors.forename: Sumeet K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research, Dr Homi Bhabha Road, Pune-411021, India. hn.gopi@iiserpune.ac.in.
authors.forename: Rajkumar
authors.initials: R
authors.lastname: Misra
authors.affiliation: National Center for Cell Sciences, Pune University Campus, Pune-411 007, India. anjali@nccs.res.in.
authors.forename: Anjali
authors.initials: A
authors.lastname: Shiras
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research, Dr Homi Bhabha Road, Pune-411021, India. hn.gopi@iiserpune.ac.in.
authors.forename: Hosahudya N
authors.initials: HN
authors.lastname: Gopi
mesh_terms: D000818:Animals; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D051379:Mice; D010455:Peptides / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D008081:Liposomes; D010455:Peptides