title: Non-farnesylated B-type lamin can tether chromatin inside the nucleus and its chromatin interaction requires the Ig-fold region.
abstract: Lamins are thought to direct heterochromatin to the nuclear lamina (NL); however, this function of lamin has not been clearly demonstrated in vivo. To address this, we analyzed polytene chromosome morphology when artificial lamin variants were expressed in Drosophila endoreplicating cells. We found that the CaaX-motif-deleted B-type lamin Dm0, but not A-type lamin C, was able to form a nuclear envelope-independent layer that was closely associated with chromatin. Other nuclear envelope proteins were not detected in this "ectopic lamina," and the associated chromatin showed a repressive histone modification maker but not a permissive histone modification marker nor RNA polymerase II proteins. Furthermore, deletion of the C-terminal lamin-Ig-fold domain prevents chromatin association with this ectopic lamina. Thus, non-farnesylated B-type lamin Dm0can form an ectopic lamina and induce changes to chromatin structure and status inside the interphase nucleus.
authors.affiliation: Department of Chemistry, Faculty of Science, Niigata University, Niigata, 950-2181, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Uchino
authors.affiliation: Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Sugiyama
authors.affiliation: Department of Chemistry, Faculty of Science, Niigata University, Niigata, 950-2181, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Katagiri
authors.affiliation: Department of Chemistry, Faculty of Science, Niigata University, Niigata, 950-2181, Japan.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Chuman
authors.affiliation: Department of Chemistry, Faculty of Science, Niigata University, Niigata, 950-2181, Japan. furukawa@chem.sc.niigata-u.ac.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila; D034921:Lamin Type B / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism; D034881:Nuclear Lamina; D059372:Nucleotide Motifs; D059007:Polytene Chromosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D017384:Sequence Deletion
chemical_list: D002843:Chromatin; D034921:Lamin Type B
keywords: B-type lamin; Chromatin; Ectopic lamina; Ig-fold domain; Nuclear lamina