title: The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64.
abstract: The Spitzenkörper is a dynamic and specialized multicomponent cell complex present in the tips of hyphal cells. The amphiphilic styryl dye FM4-64 was found to be ideal for imaging the dynamic changes of the apical vesicle cluster within growing hyphal tips. It is widely used as a marker of endocytosis and to visualize vacuolar membranes. Here we performed uptake experiments using FM4-64 to study the dynamic of the Spitzenkörper in Trichosporon asahii. We observed that Spitzenkörpers were present at the tip of the budding site of the spore, blastospore, and the germ tube of T. asahii. We also found that Spitzenkörpers were present at the tip of the hyphae as well as the subapical regions. Cytochalasin D, an inhibitor of actin polymerization, leads to abnormal Spitzenkörper formation and loss of cell polarity.
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, 5 Nanmencang, Dongcheng District, Beijing 100700, PR China.
authors.forename: Yuntong
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, 5 Nanmencang, Dongcheng District, Beijing 100700, PR China.
authors.forename: Zhikuan
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Department of Dermatology, General Hospital of Beijing Military Command of PLA, 5 Nanmencang, Dongcheng District, Beijing 100700, PR China. Electronic address: wangwl66@163.com.
authors.forename: Wenling
authors.initials: W
authors.lastname: Wang
mesh_terms: D005456:Fluorescent Dyes / Q000032:analysis*; D025301:Hyphae / Q000166:cytology* / Q000254:growth & development; D008856:Microscopy, Fluorescence; D015388:Organelles / Q000378:metabolism*; D011726:Pyridinium Compounds / Q000032:analysis*; D000644:Quaternary Ammonium Compounds / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*; D014250:Trichosporon / Q000166:cytology* / Q000254:growth & development*
chemical_list: C092350:FM 4-64; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds
keywords: Cytochalasin D; Polarized growth; Spitzenkörper; Trichosporon asahii