title: [Expression and prognostic value of CARD11 in diffuse large B cell lymphoma].
abstract: OBJECTIVE
  To determine the CARD11 expression and its prognostic value in diffuse large B cell lymphoma (DLBCL).
  
  
  METHODS
  This retrospective study included previously untreated patients diagnosed with DLBCL from January 2007 to December 2012. Formalin-fixed, paraffin-embedded blocks of these patients were collected. Tissue microarray was built and expression of CARD11 was examined immunohistochemically. Subtype of DLBCL was determined by Hans algorithm (CD10, BCL6, MUM1). The pattern of CARD11 was further studied and their correlation with outcome was analyzed.
  
  
  RESULTS
  79 patients with DLBCL were enrolled and two reactive lymph nodes were used as control. The positive rate of high CARD11 expression in DLBCL was 65.33%, which showed no significant associations with patients' characteristics. Positive CARD11 expression was associated with an inferior event free survival (EFS)(2- year EFS: 52.03%vs 86.12%,P=0.036). Even in patients with a high international prognostic index (IPI, 3-5 points), this difference still remained significant (Median EFS not reached vs 557 days,P=0.033).
  
  
  CONCLUSIONS
  DLBCL patients with high CARD11 expression had a shorter EFS compared with low level of CARD11. This difference remained significant when patients were in high IPI (3-5 points), which might indicate the value of CARD11 in stratification of high-risk DLBCL patients.
authors.affiliation: Department of Hematology, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Danqing
authors.initials: D
authors.lastname: Zhao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.forename: Dingrong
authors.initials: D
authors.lastname: Zhong
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D053468:CARD Signaling Adaptor Proteins / Q000235:genetics / Q000378:metabolism*; D018572:Disease-Free Survival; D006162:Guanylate Cyclase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D053468:CARD Signaling Adaptor Proteins; C426242:CARD11 protein, human; D006162:Guanylate Cyclase