title: Dynamic changes in protein interaction between AKAP95 and Cx43 during cell cycle progression of A549 cells.
abstract: Here we show that A-kinase anchoring protein 95 (AKAP95) and connexin 43 (Cx43) dynamically interact during cell cycle progression of lung cancer A549 cells. Interaction between AKAP95 and Cx43 at different cell cycle phases was examined by tandem mass spectrometry(MS/MS), confocal immunofluorescence microscopy, Western blot, and co-immunoprecipitation(Co-IP). Over the course of a complete cell cycle, interaction between AKAP95 and Cx43 occurred in two stages: binding stage from late G1 to metaphase, and separating stage from anaphase to late G1. The binding stage was further subdivided into complex binding to DNA in interphase and complex separating from DNA in metaphase. In late G1, Cx43 translocated to the nucleus via AKAP95; in anaphase, Cx43 separated from AKAP95 and aggregated between two daughter nuclei. In telophase, Cx43 aggregated at the membrane of the cleavage furrow. After mitosis, Cx43 was absent from the furrow membrane and was located in the cytoplasm. Binding between AKAP95 and Cx43 was reduced by N-(2-[P-Bromocinnamylamino]-ethyl)-5-isoquinolinesulfonmide (H89) treatment and enhanced by Forskolin. dynamic interaction between AKAP95 and Cx43 varies with cell cycle progression to regulate multiple biological processes.
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning, 116001, PR China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zhuang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Bogang
authors.initials: B
authors.lastname: Teng
authors.affiliation: The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Xiuyi
authors.initials: X
authors.lastname: Yu
authors.affiliation: Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning, 116001, PR China.
authors.forename: Suhang
authors.initials: S
authors.lastname: Hua
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Fengchao
authors.initials: F
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Suhui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zou
authors.affiliation: School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang liaoning, 110016, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Dai
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, 361102, PR China.
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D054758:A Kinase Anchor Proteins / Q000378:metabolism*; D000072283:A549 Cells; D002352:Carrier Proteins; D002467:Cell Nucleus / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D018031:Connexin 43 / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D009685:Nuclear Envelope / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021381:Protein Transport; D053719:Tandem Mass Spectrometry
chemical_list: D054758:A Kinase Anchor Proteins; C485099:AKAP8 protein, human; D002352:Carrier Proteins; D018031:Connexin 43; D005576:Colforsin; D017868:Cyclic AMP-Dependent Protein Kinases