title: Detection and genome sequence of a new betapartitivirus associated with Cucurbitaria piceae Borthw. fungus causing bud blight of spruce in the Czech Republic.
abstract: A new bisegmented dsRNA virus has been detected in shoots of blue spruce with bud blight disease symptoms and infection by Cucurbitaria piceae (Bortw.) fungus. The virus genome consists of two segments that are 2071 and 2257 nt long, encoding the putative RNA polymerase and capsid protein, respectively. Rosellinia necatrix partitivirus 1 is a closely related virus with 45 % amino acid sequence identity in the polymerase, and crimson clover cryptic virus 2 has 36 % amino acid sequence identity in the capsid protein. Based on taxonomic criteria, the virus should be classified as a member of the genus Betapartitivirus (family Partitiviridae). The name Cucurbitaria piceae partitivirus 1 and acronym CpPV1 are proposed.
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic. petrzik@umbr.cas.cz.
authors.forename: Karel
authors.identifier: http://orcid.org/0000-0001-9668-1223
authors.initials: K
authors.lastname: Petrzik
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic.
authors.forename: Igor
authors.initials: I
authors.lastname: Koloniuk
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Sarkisova
authors.affiliation: Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Hrabáková
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000821:virology*; D001483:Base Sequence; D018153:Czech Republic; D000067148:Fungal Viruses / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D028222:Picea / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology / Q000821:virology*; D018520:Plant Shoots / Q000382:microbiology / Q000821:virology