title: Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers. II. Loading of the Hemoderivative Platelet Lysate.
abstract: Platelet lysate (PL) was loaded into dressings based on chitosan glutamate (CSG) low and high molecular weight, sericin (Ser), and glycine (Gly). A synergic effect of Ser and PL on fibroblast proliferation was proved in vitro. Two different PL loading approaches were considered: the first provided to prepare dressings by freeze-drying a mixture of PL and CSG/Gly/Ser solution, the second approach consisted in the extemporarily loading of PL in the CSG/Gly/Ser freeze-dried dressings. As for the first approach, PL loading did not produce any variation in dressing mechanical properties. Such dressings absorbed a high amount (about 8-fold of dry weight) of phosphate-buffered saline (fluid mimicking wound exudate), forming a gel with pseudoplastic and elastic properties. Platelet-derived growth factor AB assay indicated that neither freeze-drying nor the excipients alter PL growth factor content. As for the second approach, mechanical and rheological properties of the gel formed upon PL absorption enabled to choose a PL loading of about 90 μL/cm(2). Upon contact with fibroblasts, all PL loaded formulations increased the number not only of viable cells but also of those in the proliferative phase. Histological studies effected on human skin strips pointed out the positive effect of PL loaded dressings on dermal matrix reconstruction.
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Mori
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy. Electronic address: silvia.rossi@unipv.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Bonferoni
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, Histology and Embryology Unit, University of Pavia, Pavia, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Marika
authors.initials: M
authors.lastname: Tenci
authors.affiliation: Immunohaematology and Transfusion Service and Cell Therapy Unit of Fondazione IRCCS, S. Matteo, Pavia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Del Fante
authors.affiliation: Plastic and Reconstructive Surgery Unit, Department of Clinical Surgical Diagnostic and Pediatric Sciences, University of Pavia, Salvatore Maugeri Research and Care Institute, Pavia, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Nicoletti
authors.affiliation: Department of Drug Science, University of Pavia, Pavia, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001458:Bandages; D001792:Blood Platelets / Q000737:chemistry* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005612:Freeze Drying / Q000379:methods; D018698:Glutamic Acid / Q000737:chemistry / Q000494:pharmacology; D005998:Glycine / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D047030:Sericins / Q000737:chemistry* / Q000494:pharmacology; D012883:Skin Ulcer / Q000178:diet therapy* / Q000378:metabolism; D012996:Solutions / Q000737:chemistry / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D010982:Platelet-Derived Growth Factor; D047030:Sericins; D012996:Solutions; C095477:platelet-derived growth factor AB; D018698:Glutamic Acid; D048271:Chitosan; D005998:Glycine
keywords: biomaterials; cell culture; chitosan; skin; wound healing