title: Effects of 36.6 GHz and static magnetic field on degree of endoreduplication in Drosophila melanogaster polytene chromosomes.
abstract: Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (frequency - 36.64 GHz, power density - 1 W/m(2), exposure time - 30 sec) and static magnetic field (intensity - 25 mT, exposure time - 5 min) applied at the egg stage after a 2-h oviposition. Giant chromosomes were investigated in squashed preparations of the salivary glands stained by acetoorcein by the cytomorphometric method. Preparations were obtained from Drosophila larvae at the 0 h prepupae stage. Results Exposure to microwaves increased the degree of polyteny in chromosomes (DPC) by 7.5%, and the statistical power of the impact was: h(2) = 35.3%. A similar effect occurred after the sequential action of microwaves and static magnetic field: The polyteny level of chromosomes increased by 7.4%, statistical power was: h(2) = 30.6%. Conclusions Exposure to microwaves on the stage of embryogenesis has a stimulating effect on endoreduplication in Drosophila development. The effect of microwaves was not modified by the action of the static magnetic field.
authors.affiliation: a Institute of Biology , V. N. Karazin Kharkiv National University , Kharkiv , Ukraine.
authors.forename: Liliia D
authors.initials: LD
authors.lastname: Dyka
authors.affiliation: a Institute of Biology , V. N. Karazin Kharkiv National University , Kharkiv , Ukraine.
authors.forename: Lyubov A
authors.initials: LA
authors.lastname: Shakina
authors.affiliation: a Institute of Biology , V. N. Karazin Kharkiv National University , Kharkiv , Ukraine.
authors.forename: Volodymyr Yu
authors.initials: VY
authors.lastname: Strashnyuk
authors.affiliation: a Institute of Biology , V. N. Karazin Kharkiv National University , Kharkiv , Ukraine.
authors.forename: Yuriy G
authors.initials: YG
authors.lastname: Shckorbatov
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004331:Drosophila melanogaster / Q000235:genetics* / Q000528:radiation effects*; D062951:Endoreduplication / Q000235:genetics* / Q000528:radiation effects; D060526:Magnetic Fields; D008872:Microwaves; D059007:Polytene Chromosomes / Q000235:genetics* / Q000528:radiation effects; D011829:Radiation Dosage
keywords: Chromatin; cell cycle effects; degree of polyteny; giant chromosomes; non-ionizing radiations