title: Prognostic significance of tissue miR-345 downregulation in non-small cell lung cancer.
abstract: BACKGROUND
  MiRNAs might function as oncogenes or tumor suppressor genes in the tumorigenesis process. Dysregulation of miR-345 is a frequent event in many types of human cancers. However, the tissue miR-345 expression level in non-small cell lung cancer (NSCLC) and its potential clinical significance remains unknown.
  
  
  METHODS
  Real-time PCR was conducted to evaluate the expression level of miR-345 in NSCLC tissues as well as cell lines. Then the association between tissue miR-345 expression level and clinical outcome was investigated.
  
  
  RESULTS
  The expression level of miR-345 was significantly decreased in NSCLC tissues and cell lines compared with the controls (P<0.05; P<0.01). Tissue miR-345 expression level was associated with various clinicopathological parameters including LN metastasis (P=0.012), distant metastasis (P=0.007), TNM stage (P=0.008) and grade (P=0.030). In addition, the NSCLC patients in thelow tissue miR-345 expression group had significantly shorter 5-year overall survival time than those in the high tissue miR-345expression group (P=0.016). Multivariate analysis showed that tissue miR-345 was an independent risk factor for NSCLC (HR=3.921, 95% CI: 2.285-10.540; P=0.008).
  
  
  CONCLUSIONS
  The expression level of miR-345 was reduced in NSCLC tissues and cell lines. Low tissue miR-345 expression was associated with progression and poor prognosis of NSCLC, indicating that tissue miR-345 may serve as a novel prognostic marker in NSCLC.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Shantou University Medical College Shantou, Guangdong, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Chen
keywords: Biomarker; MiR-345; non-small cell lung cancer; prognosis