title: MicroRNA-410-5p as a potential serum biomarker for the diagnosis of prostate cancer.
abstract: BACKGROUND
  Prostate cancer (PCa) remains to be a diagnostic challenge due to its variable presentation and the lack of reliable diagnosis tool. MicroRNAs (miRNAs) regulate gene in extensive range of pathophysiologic processes. Plasma miRNAs are ideal biomarkers in heart failure, diabetes and other disease. However, using circulating miRNAs as biomarkers for the diagnosis of PCa is still unknown.
  
  
  METHODS
  149 PCa patients, 57 healthy controls, and 121 non-cancer patients (benign prostatic hyperplasia and other urinary diseases) were enrolled in this study. The reverse transcription of miRNA and SYBR-Green-based double standards curve miRNA quantitative polymerase chain reactions (qPCR) were used to evaluate the dysregulated miR-410-5p. Receiver operator characteristic (ROC) curve analysis was used to evaluate the diagnostic accuracy of miR-410-5p identified as the alternative biomarker.
  
  
  RESULTS
  Circulating miRNA-410-5p (miR-410-5p) level was significantly higher in the PCa patients than in healthy controls or non-cancer patients. ROC curve analysis showed that plasma miR-410-5p was a specific diagnostic biomarker of PCa with an area under curve(AUC) of 0.8097 (95 % confidence interval, 0.7371-0.8823; P < 0.001).
  
  
  CONCLUSIONS
  The serum miR-410-5p level is a potential biomarker for the diagnosis of PCa.
authors.affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Urology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Huamao
authors.initials: H
authors.lastname: Ye
authors.affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Xiya
authors.initials: X
authors.lastname: Yu
authors.affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Departments of Nuclear Medicine, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Changjing
authors.initials: C
authors.lastname: Zuo
authors.affiliation: Department of Pathology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Yongwei
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Guixia
authors.initials: G
authors.lastname: Xu
authors.affiliation: Clinical Research Center, Changhai Hospital, Second Military Medical University, 168 Changhai Road, 200433 Shanghai, China.
authors.forename: Shanrong
authors.initials: S
authors.lastname: Liu
keywords: Circulating miRNA; Diagnosis; Prostate cancer; miR-410-5p