title: Ferulic Acid against Cyclophosphamide-Induced Heart Toxicity in Mice by Inhibiting NF-κB Pathway.
abstract: The purpose of the present study was to elucidate the protective effects of ferulic acid (FA) against cyclophosphamide- (CTX-) induced changes in mice. Forty-eight male ICR mice were divided into four groups. Control group was intraperitoneally (i.p.) injected with 200 μL of phosphate buffer saline (PBS). Model group was intraperitoneally injected with a single dose of CTX (200 mg/kg). FA (50 mg/kg) and FA (100 mg/kg) groups were intraperitoneally injected with a single dose of CTX (200 mg/kg) followed by the intragastric treatment with FA (50, 100 mg/kg) for 7 consecutive days. After 12 d, the mice were sacrificed to analyze the hematological, biochemical, histological parameters and mechanism research. The results indicated that FA significantly decreased the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) in CTX-injected mice. In addition, FA effectively reduced the total numbers of white blood cells (WBCs), red blood cells, platelets, and hemoglobin content. FA also obviously attenuated the histological changes of the heart tissues caused by CTX. Moreover, Western blot demonstrated that FA inhibited the phosphorylations of NF-κB signaling pathway in CTX-stimulated cardiac tissues. In conclusion, FA might be considered as an effective agent in the amelioration of the heart toxicity resulting from CTX treatment.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Yafan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Congxia
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Oncology, The Central Hospital of Xi'an, Xi'an 710003, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Oncology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Ma