title: NF45 and NF90 Bind HIV-1 RNA and Modulate HIV Gene Expression.
abstract: A previous proteomic screen in our laboratory identified nuclear factor 45 (NF45) and nuclear factor 90 (NF90) as potential cellular factors involved in human immunodeficiency virus type 1 (HIV-1) replication. Both are RNA binding proteins that regulate gene expression; and NF90 has been shown to regulate the expression of cyclin T1 which is required for Tat-dependent trans-activation of viral gene expression. In this study the roles of NF45 and NF90 in HIV replication were investigated through overexpression studies. Ectopic expression of either factor potentiated HIV infection, gene expression, and virus production. Deletion of the RNA binding domains of NF45 and NF90 diminished the enhancement of HIV infection and gene expression. Both proteins were found to interact with the HIV RNA. RNA decay assays demonstrated that NF90, but not NF45, increased the half-life of the HIV RNA. Overall, these studies indicate that both NF45 and NF90 potentiate HIV infection through their RNA binding domains.
authors.affiliation: Department of Medical Microbiology and Immunology, Creighton University, Omaha, NE 68178, USA. yanli@creighton.edu.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Medical Microbiology and Immunology, Creighton University, Omaha, NE 68178, USA. michaelbelshan@creighton.edu.|The Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583, USA. michaelbelshan@creighton.edu.
authors.forename: Michael
authors.initials: M
authors.lastname: Belshan
mesh_terms: D015967:Gene Expression Regulation, Viral; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D051903:Nuclear Factor 45 Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D050779:Nuclear Factor 90 Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C494013:ILF2 protein, human; C429643:ILF3 protein, human; D051903:Nuclear Factor 45 Protein; D050779:Nuclear Factor 90 Proteins; D012367:RNA, Viral; D014764:Viral Proteins
keywords: HIV; NF45; NF90; RNA regulation; transcription