title: Induction of TDO2 and IDO2 in Liver by High-Fat Feeding in Mice: Discrepancies with Human Obesity.
abstract: Low-grade and chronic inflammation is elicited in white adipose tissue in human obesity. The presence of inflammatory molecules leads to an increased tryptophan catabolism through the induction of indoleamine-2,3-dioxygenase-1 (IDO1). In order to characterize the mechanisms underlying this dysregulation, we have studied 2 mouse models of obesity. Unexpectedly, we did not detect any IDO1 expression in obese or lean mice adipose tissue. In a previous study, we did not find any significant difference in the liver for IDO2 and tryptophan-2,3-dioxygenase (TDO2) gene expression between normal weight and obese patients. IDO2 and TDO2 expression was increased in the liver of high-fat fed mice, but not in ob/ob mice, and was strongly correlated with hydroxysteroid-(11-beta) dehydrogenase-1 (HSD11B1) expression, an enzyme that generates active cortisol within tissues. In conclusion, despite a dysregulation of tryptophan metabolism, obese mice display discrepancies with human obesity metabolism, rendering them inappropriate for further investigations in this animal model.
authors.affiliation: European Genomic Institute for Diabetes (EGID), Lille, France.; University of Lille, Lille, France.; CNRS UMR 8199, Lille, France.; CHRU Lille, Lille, France.; IPL, Lille, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Poulain-Godefroy
authors.affiliation: European Genomic Institute for Diabetes (EGID), Lille, France.; University of Lille, Lille, France.; CNRS UMR 8199, Lille, France.; CHRU Lille, Lille, France.; IPL, Lille, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Eury
authors.affiliation: European Genomic Institute for Diabetes (EGID), Lille, France.; University of Lille, Lille, France.; CNRS UMR 8199, Lille, France.; CHRU Lille, Lille, France.; IPL, Lille, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Leloire
authors.affiliation: University of Lille, Lille, France.; CHRU Lille, Lille, France.; EA4483, Faculty of Medicine, Lille, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hennart
authors.affiliation: MND and Neurodegenerative Diseases Research Group, Australian School of Advanced Medicine, Macquarie University, Australia.
authors.forename: Gilles J
authors.initials: GJ
authors.lastname: Guillemin
authors.affiliation: University of Lille, Lille, France.; CHRU Lille, Lille, France.; EA4483, Faculty of Medicine, Lille, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Allorge
authors.affiliation: European Genomic Institute for Diabetes (EGID), Lille, France.; University of Lille, Lille, France.; CNRS UMR 8199, Lille, France.; CHRU Lille, Lille, France.; IPL, Lille, France.; Department of Genomics of Common Disease, School of Public Health, Imperial College London, United Kingdom.
authors.forename: Philippe
authors.initials: P
authors.lastname: Froguel
keywords: 3-dioxygenase; 3-dioxygenase 2; high fat diet; indoleamine 2; obesity; tryptophan 2