title: Isolation and identification of berberine and berberrubine metabolites by berberine-utilizing bacterium Rhodococcus sp. strain BD7100.
abstract: Based on the finding of a novel berberine (BBR)-utilizing bacterium, Rhodococcus sp. strain BD7100, we investigated the degradation of BBR and its analog berberrubine (BRU). Resting cells of BD7100 demethylenated BBR and BRU, yielding benzeneacetic acid analogs. Isolation of benzeneacetic acid analogs suggested that BD7100 degraded the isoquinoline ring of the protoberberine skeleton. This work represents the first report of cleavage of protoberberine skeleton by a microorganism.
authors.affiliation: a Department of Organic Chemistry , Hoshi University , Tokyo , Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Ishikawa
authors.affiliation: a Department of Organic Chemistry , Hoshi University , Tokyo , Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Takeda
authors.affiliation: a Department of Organic Chemistry , Hoshi University , Tokyo , Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Wakana
authors.affiliation: b Division of Integrated Life Science, Graduate School of Biostudies , Kyoto University , Kyoto , Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Sato
authors.affiliation: a Department of Organic Chemistry , Hoshi University , Tokyo , Japan.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Hosoe
mesh_terms: D001599:Berberine / Q000031:analogs & derivatives / Q000378:metabolism*; D001600:Berberine Alkaloids / Q000378:metabolism; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D010648:Phenylacetates / Q000378:metabolism; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001600:Berberine Alkaloids; D003470:Culture Media; D010648:Phenylacetates; D001599:Berberine; C115958:berberrubine; C009090:protoberberine
keywords: Rhodococcus sp; benzeneacetic acid analog; berberine; berberrubine; degradation