title: Divergent responses of methanogenic archaeal communities in two rice cultivars to elevated ground-level O3.
abstract: Inhibitive effect of elevated ground-level ozone (O3) on paddy methane (CH4) emission varies with rice cultivars. However, little information is available on its microbial mechanism. For this purpose, the responses of methane-metabolizing microorganisms, methanogenic archaea and methanotrophic bacteria to O3 pollution were investigated in the O3-tolerant (YD6) and the O3-sensitive (IIY084) cultivars at two rice growth stages in Free Air Concentration Elevation of O3 (O3-FACE) system of China. It was found that O3 pollution didn't change the abundances of Type I and Type II methanotrophic bacteria at two rice stages. For methanogenic archaea, their abundances in both cultivars were decreased by O3 pollution at the tillering stage. Furthermore, a greater negative influence on methanogenic archaeal community was observed on IIY084 than on YD6: at tillering stage, the alpha diversity indices of methanogenic archaeal community in IIY084 was decreased to a greater extent than in YD6; IIY084 shifted methanogenic archaeal community composition and decreased the abundances and the diversities of Methanosarcinaceae and Methanosaetaceae as well as the abundance of Methanomicrobiales, while the diversity of Methanocellaceae were increased in YD6. These findings indicate that the variations in the responses of paddy CH4 emission to O3 pollution between cultivars could result from the divergent responses of their methanogenic archaea.
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China.
authors.forename: Haoye
authors.initials: H
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China.
authors.forename: Xiangui
authors.initials: X
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China. Electronic address: yzfeng@issas.ac.cn.
authors.forename: Youzhi
authors.initials: Y
authors.lastname: Feng
mesh_terms: D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D002681:China; D014644:Genetic Variation; D008697:Methane / Q000378:metabolism*; D012275:Oryza / Q000235:genetics* / Q000382:microbiology*; D010126:Ozone / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D010126:Ozone; D008697:Methane
keywords: Elevated ground-level ozone; Methanogenic archaea; Methanotrophic bacteria; Rice cultivar