title: Up-regulation of TDAG51 is a dependent factor of LPS-induced RAW264.7 macrophages proliferation and cell cycle progression.
abstract: BACKGROUND
  As a component of the outer membrane in Gram-negative bacteria, lipopolysaccharide (LPS)-induced proliferation and cell cycle progression of monocytes/macrophages. It has been suggested that the proapoptotic T-cell death-associated gene 51 (TDAG51) might be associated with cell proliferation and cell cycle progression; however, its role in the interaction between LPS and macrophages remains unclear.
  
  
  OBJECTIVE
  We attempted to elucidate the role(s) of TDAG51 played in the interaction between LPS and macrophages.
  
  
  METHODS
  We investigated TDAG51 expression in RAW264.7 cells stimulated with LPS and examined the effects of RNA interference-mediated TDAG51 down-regulation. We used CCK-8 assay and flow cytometry analysis to evaluate the interaction between TDAG51 and LPS-induced proliferation and cell cycle progression in RAW264.7 cells.
  
  
  RESULTS
  Our findings indicate that TDAG51 is up-regulated in LPS-stimulated RAW264.7 cells, the TDAG51 siRNA effectively reduced TDAG51 protein up-regulation following LPS stimulation in RAW264.7 cells, the significant changes of the proliferation and cell cycle progression of RAW264.7 cells in TDAG51 Knockdown RAW264.7 cells treated with LPS were observed.
  
  
  CONCLUSIONS
  These findings suggested that TDAG51 up-regulation is a dependent event during LPS-mediated proliferation and cell cycle progression, and which increase our understanding of the interaction mechanism between LPS and macrophages.
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Han-Wei
authors.initials: HW
authors.lastname: Jiao
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Jia
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Tian-Jing
authors.initials: TJ
authors.lastname: Zhao
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Hui
authors.initials: H
authors.lastname: Rong
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Jia-Ning
authors.initials: JN
authors.lastname: Zhang
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Hua-Pei
authors.initials: HP
authors.lastname: Zhu
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Kai-Lian
authors.initials: KL
authors.lastname: Xu
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Shi-Yu
authors.initials: SY
authors.lastname: Guo
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Qiao-Yun
authors.initials: QY
authors.lastname: Shi
authors.affiliation: b College of Animal Science and Technology, Shihezi University , North 4th Road, Shihezi , People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Feng-Yang
authors.initials: FY
authors.lastname: Wang
authors.affiliation: b College of Animal Science and Technology, Shihezi University , North 4th Road, Shihezi , People's Republic of China.
authors.forename: Chuang-Fu
authors.initials: CF
authors.lastname: Chen
authors.affiliation: a College of Agriculture, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou , Haidian Island , Haikou , People's Republic of China and.
authors.forename: Li
authors.initials: L
authors.lastname: Du
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D014157:Transcription Factors / Q000096:biosynthesis*; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D008070:Lipopolysaccharides; C114072:Phlda1 protein, mouse; D014157:Transcription Factors
keywords: Cell cycle; LPS; TDAG51; macrophage; proliferation