title: The Interaction between Adult Cardiac Fibroblasts and Embryonic Stem Cell-Derived Cardiomyocytes Leads to Proarrhythmic Changes in In Vitro Cocultures.
abstract: Transplantation of stem cell-derived cardiomyocytes is one of the most promising therapeutic approaches after myocardial infarction, as loss of cardiomyocytes is virtually irreversible by endogenous repair mechanisms. In myocardial scars, transplanted cardiomyocytes will be in immediate contact with cardiac fibroblasts. While it is well documented how the electrophysiology of neonatal cardiomyocytes is modulated by cardiac fibroblasts of the same developmental stage, it is unknown how adult cardiac fibroblasts (aCFs) affect the function of embryonic stem cell-derived cardiomyocytes (ESC-CMs). To investigate the effects of aCFs on ESC-CM electrophysiology, we performed extra- and intracellular recordings of murine aCF-ESC-CM cocultures. We observed that spontaneous beating behaviour was highly irregular in aCF-ESC-CM cocultures compared to cocultures with mesenchymal stem cells (coefficient of variation of the interspike interval: 40.5 ± 15.2% versus 9.3 ± 2.0%, p = 0.008) and that action potential amplitude and maximal upstroke velocity (V max) were reduced (amplitude: 52.3 ± 1.7 mV versus 65.1 ± 1.5 mV, V max: 7.0 ± 1.0 V/s versus 36.5 ± 5.3 V/s), while action potential duration (APD) was prolonged (APD50: 25.6 ± 1.0 ms versus 16.8 ± 1.9 ms, p < 0.001; APD90: 52.2 ± 1.5 ms versus 43.3 ± 3.3 ms, p < 0.01) compared to controls. Similar changes could be induced by aCF-conditioned medium. We conclude that the presence of aCFs changes automaticity and induces potentially proarrhythmic changes of ESC-CM electrophysiology.
authors.affiliation: Department of Paediatric Cardiology, Heart Centre Cologne, University Hospital of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany; Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Trieschmann
authors.affiliation: Department of Paediatrics, University Hospital of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bettin
authors.affiliation: Department of Paediatric Cardiology, Heart Centre Cologne, University Hospital of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany.
authors.forename: Moritz
authors.initials: M
authors.lastname: Haustein
authors.affiliation: Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Köster
authors.affiliation: Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.
authors.forename: Marek
authors.initials: M
authors.lastname: Molcanyi
authors.affiliation: Department of Internal Medicine III, Heart Centre Cologne, University of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Halbach
authors.affiliation: Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany; Physiology Department, Faculty of Medicine (Kasr El-Aini), Cairo University, El-Maniel, Cairo 11451, Egypt.
authors.forename: Mira
authors.initials: M
authors.lastname: Hanna
authors.affiliation: Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.
authors.forename: Mariam
authors.initials: M
authors.lastname: Fouad
authors.affiliation: Department of Paediatric Cardiology, Heart Centre Cologne, University Hospital of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany.
authors.forename: Konrad
authors.initials: K
authors.lastname: Brockmeier
authors.affiliation: Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hescheler
authors.affiliation: Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.
authors.forename: Kurt
authors.initials: K
authors.lastname: Pfannkuche
authors.affiliation: Department of Paediatric Cardiology, Heart Centre Cologne, University Hospital of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany; Institute for Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany; Department of Paediatrics, University Hospital of Cologne, Kerpener Strasse 62, 50937 Cologne, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Hannes