title: Characterization of quinolone resistance in Salmonella spp. isolates from food products and human samples in Brazil.
abstract: Non-typhoidal salmonellosis is an important zoonotic disease caused by Salmonella enterica. The aim of this study was to investigate the prevalence of plasmid-mediated quinolone resistance in Salmonella spp. and its association with fluoroquinolone susceptibility in Brazil. A total of 129 NTS isolates (samples from human origin, food from animal origin, environmental, and animal) grouped as from animal (n=62) and human (n=67) food were evaluated between 2009 and 2013. These isolates were investigated through serotyping, antimicrobial susceptibility testing, and the presence of plasmid-mediated quinolone resistance (PMQR) genes (qnr, aac(6')-Ib) and associated integron genes (integrase, and conserved integron region). Resistance to quinolones and/or fluoroquinolones, from first to third generations, was observed. Fifteen isolates were positive for the presence of qnr genes (8 qnrS, 6 qnrB, and 1 qnrD) and twenty three of aac(6')-Ib. The conserved integron region was detected in 67 isolates as variable regions, from ±600 to >1000pb. The spread of NTS involving PMQR carriers is of serious concern and should be carefully monitored.
authors.affiliation: Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil; Laboratório de bacteriologia, Departamento de microbiologia e Imunologia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil. Electronic address: bruno.pribul@ioc.fiocruz.br.
authors.forename: Bruno Rocha
authors.initials: BR
authors.lastname: Pribul
authors.affiliation: Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Marcia Lima
authors.initials: ML
authors.lastname: Festivo
authors.affiliation: Laboratório de bacteriologia, Departamento de microbiologia e Imunologia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Miliane Moreira Soares
authors.initials: MM
authors.lastname: de Souza
authors.affiliation: Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Dalia dos Prazeres
authors.initials: Ddos P
authors.lastname: Rodrigues
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D024881:Drug Resistance, Bacterial; D004783:Environmental Microbiology; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D032023:Integrons; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000032:analysis; D015363:Quinolones / Q000494:pharmacology*; D012480:Salmonella Infections / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones
keywords: Foodborne diseases; Quinolone resistance; Salmonella spp.