title: Guidance of Axons by Local Coupling of Retrograde Flow to Point Contact Adhesions.
abstract: Growth cones interact with the extracellular matrix (ECM) through integrin receptors at adhesion sites termed point contacts. Point contact adhesions link ECM proteins to the actin cytoskeleton through numerous adaptor and signaling proteins. One presumed function of growth cone point contacts is to restrain or "clutch" myosin-II-based filamentous actin (F-actin) retrograde flow (RF) to promote leading edge membrane protrusion. In motile non-neuronal cells, myosin-II binds and exerts force upon actin filaments at the leading edge, where clutching forces occur. However, in growth cones, it is unclear whether similar F-actin-clutching forces affect axon outgrowth and guidance. Here, we show in Xenopus spinal neurons that RF is reduced in rapidly migrating growth cones on laminin (LN) compared with non-integrin-binding poly-d-lysine (PDL). Moreover, acute stimulation with LN accelerates axon outgrowth over a time course that correlates with point contact formation and reduced RF. These results suggest that RF is restricted by the assembly of point contacts, which we show occurs locally by two-channel imaging of RF and paxillin. Further, using micropatterns of PDL and LN, we demonstrate that individual growth cones have differential RF rates while interacting with two distinct substrata. Opposing effects on RF rates were also observed in growth cones treated with chemoattractive and chemorepulsive axon guidance cues that influence point contact adhesions. Finally, we show that RF is significantly attenuated in vivo, suggesting that it is restrained by molecular clutching forces within the spinal cord. Together, our results suggest that local clutching of RF can control axon guidance on ECM proteins downstream of axon guidance cues.
  
  
  
  Here, we correlate point contact adhesions directly with clutching of filamentous actin retrograde flow (RF), which our findings strongly suggest guides developing axons. Acute assembly of new point contact adhesions is temporally and spatially linked to attenuation of RF at sites of forward membrane protrusion. Importantly, clutching of RF is modulated by extracellular matrix (ECM) proteins and soluble axon guidance cues, suggesting that it may regulate axon guidance in vivo. Consistent with this notion, we found that RF rates of spinal neuron growth cones were slower in vivo than what was observed in vitro. Together, our study provides the best evidence that growth cone-ECM adhesions clutch RF locally to guide axons in vivo.
authors.affiliation: Neuroscience Training Program and.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Nichol
authors.affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Kate M
authors.initials: KM
authors.lastname: Hagen
authors.affiliation: Department of Neuroscience, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Derek C
authors.initials: DC
authors.lastname: Lumbard
authors.affiliation: Neuroscience Training Program and Department of Neuroscience, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Erik W
authors.initials: EW
authors.lastname: Dent
authors.affiliation: Neuroscience Training Program and Department of Neuroscience, University of Wisconsin, Madison, Wisconsin 53706 tmgomez@wisc.edu.
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Gómez
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D001369:Axons / Q000502:physiology*; D002448:Cell Adhesion; D020439:Growth Cones / Q000502:physiology; D007797:Laminin / Q000494:pharmacology; D009474:Neurons / Q000502:physiology; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D013116:Spinal Cord / Q000166:cytology; D014982:Xenopus laevis
chemical_list: D000199:Actins; D007797:Laminin; D011107:Polylysine
keywords: actin retrograde flow; axon guidance; growth cone; kabiramide C; molecular clutch; point contact adhesion