title: Comparative assay of Vipera ammodytes antivenom potency.
abstract: The finding of the most appropriate way to assess precisely the antivenom efficacy represents one of the major issues for antivenom standardization and success increasing of antivenom therapy. The efficacy of experimental Vipera ammodytes antivenom raised in sheep was determined using in vivo mouse lethality test, respectively, L-aminoacid oxidase, total proteinase and phospholipase A2 antienzymatic effectiveness. The values gained for the antivenom potency depend on the method of measure. So, some of the most toxic venom proteins own phospholipase A2 activity and provide the highest antivenom potency (lowest effective dose) values by antienzymatic assay method. This value is similar with total antiproteolytic antivenom potency value, but almost three times higher than value obtained by L-aminoacid oxidase (low toxic viper venom protein) antienzymatic assay method.
authors.affiliation: a S.C. Ecodet Activ S. R. L , Bucharest , Romania .|b National Environmental Protection Agency , Bucharest , Romania .
authors.forename: Cristian
authors.initials: C
authors.lastname: Capitanescu
authors.affiliation: c Carol Davila University of Medicine and Pharmacy , Bucharest , Romania .
authors.forename: Anca Monica
authors.initials: AM
authors.lastname: Macovei Oprescu
authors.affiliation: d Romanian National Sanitary Veterinary and Food Safety Authority , Bucharest , Romania .
authors.forename: Alexandru
authors.initials: A
authors.lastname: Supeanu
authors.affiliation: e University "Titu Maiorescu" , Bucharest , Romania , and.
authors.forename: Bogdan Ioan
authors.initials: BI
authors.lastname: Coculescu
authors.affiliation: c Carol Davila University of Medicine and Pharmacy , Bucharest , Romania .
authors.forename: Victor
authors.initials: V
authors.lastname: Strambu
authors.affiliation: c Carol Davila University of Medicine and Pharmacy , Bucharest , Romania .
authors.forename: Radu Alexandru
authors.initials: RA
authors.lastname: Macovei
authors.affiliation: e University "Titu Maiorescu" , Bucharest , Romania , and.|f Clinical Hospital Colentina , Bucharest , Romania.
authors.forename: Gheorghe
authors.initials: G
authors.lastname: Manole
mesh_terms: D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D051379:Mice; D054467:Phospholipases A2 / Q000378:metabolism; D012756:Sheep; D014757:Viper Venoms / Q000037:antagonists & inhibitors* / Q000201:enzymology; D017819:Viperidae
chemical_list: D000997:Antivenins; D014757:Viper Venoms; D054467:Phospholipases A2
keywords: L-aminoacid oxidase; Median effective dose; phospholipase A2