title: Breaking up prolonged sitting time with walking does not affect appetite or gut hormone concentrations but does induce an energy deficit and suppresses postprandial glycaemia in sedentary adults.
abstract: Breaking up periods of prolonged sitting can negate harmful metabolic effects but the influence on appetite and gut hormones is not understood and is investigated in this study. Thirteen sedentary (7 female) participants undertook three 5-h trials in random order: (i) uninterrupted sitting (SIT), (ii) seated with 2-min bouts of light-intensity walking every 20 min (SIT + LA), and (iii) seated with 2-min bouts of moderate-intensity walking every 20 min (SIT + MA). A standardised test drink was provided at the start of each trial and an ad libitum pasta test meal provided at the end of each trial. Subjective appetite ratings and plasma acylated ghrelin, peptide YY, insulin, and glucose were measured at regular intervals. Area under the curve (AUC) was calculated for each variable. AUC values for appetite and gut hormone concentrations were unaffected in the activity breaks conditions compared with uninterrupted sitting (linear mixed modelling: p > 0.05). Glucose AUC was lower in SIT + MA than in SIT + LA (p = 0.004) and SIT (p = 0.055). There was no difference in absolute ad libitum energy intake between conditions (p > 0.05); however, relative energy intake was lower in SIT + LA (39%; p = 0.011) and SIT + MA (120%; p < 0.001) than in SIT. In conclusion, breaking up prolonged sitting does not alter appetite and gut hormone responses to a meal over a 5-h period. Increased energy expenditure from activity breaks could promote an energy deficit that is not compensated for in a subsequent meal.
authors.affiliation: a Institute for Sport and Physical Activity Research, Department of Sport Science and Physical Activity, University of Bedfordshire, Polhill Avenue, Bedford, MK41 9EA, UK.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Bailey
authors.affiliation: b Academy of Sport and Physical Activity, Sheffield Hallam University, Sheffield, S10 2BP, UK.
authors.forename: David R
authors.initials: DR
authors.lastname: Broom
authors.affiliation: a Institute for Sport and Physical Activity Research, Department of Sport Science and Physical Activity, University of Bedfordshire, Polhill Avenue, Bedford, MK41 9EA, UK.
authors.forename: Bryna C R
authors.initials: BC
authors.lastname: Chrismas
authors.affiliation: a Institute for Sport and Physical Activity Research, Department of Sport Science and Physical Activity, University of Bedfordshire, Polhill Avenue, Bedford, MK41 9EA, UK.
authors.forename: Lee
authors.initials: L
authors.lastname: Taylor
authors.affiliation: a Institute for Sport and Physical Activity Research, Department of Sport Science and Physical Activity, University of Bedfordshire, Polhill Avenue, Bedford, MK41 9EA, UK.
authors.forename: Edward
authors.initials: E
authors.lastname: Flynn
authors.affiliation: a Institute for Sport and Physical Activity Research, Department of Sport Science and Physical Activity, University of Bedfordshire, Polhill Avenue, Bedford, MK41 9EA, UK.
authors.forename: John
authors.initials: J
authors.lastname: Hough
mesh_terms: D000293:Adolescent; D000328:Adult; D001069:Appetite Regulation; D019540:Area Under Curve; D015415:Biomarkers; D001786:Blood Glucose / Q000378:metabolism*; D018592:Cross-Over Studies; D002149:Energy Intake; D004734:Energy Metabolism; D004739:England; D005260:Female; D005768:Gastrointestinal Hormones / Q000097:blood*; D006801:Humans; D006943:Hyperglycemia / Q000097:blood / Q000175:diagnosis / Q000517:prevention & control*; D007328:Insulin / Q000097:blood; D008297:Male; D062407:Meals; D019518:Postprandial Period; D057185:Sedentary Behavior; D013997:Time Factors; D016138:Walking; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D005768:Gastrointestinal Hormones; D007328:Insulin
keywords: activity breaks; acylated ghrelin; appetite-regulating hormones; apport énergétique; comportement sédentaire; energy intake; ghréline acylée; hormones régulant l’appétit; pauses actives; position assise prolongée; prolonged sitting; sedentary behaviour