title: [Bioactive glass 45S5-silk fibroin membrane supports proliferation and differentiation of human dental pulp stem cells].
abstract: OBJECTIVE
  To investigate the effect of bioactivity glass 45S5- silk fibroin(BG45S5- SF) membrane on growth, proliferation and differentiation of human dental pulp stem cells(hDPSC), and to provide new ideas and method for the regeneration of pulp-dentine complex.
  
  
  METHODS
  hDPSC seed on pure silk fibroin membrane (protein membrane group) and BG45S5-SF membrane with different concentrations(1 000, 5 000 mg/L, composite membrane group A and B, respectively) were prepared, and the materials were incubated in cell culture fluid for 24 h. No material membrane orifice plate was used as blank control group. Contact angle meter was used to measure surface contact angle of protein membrane and composite membrane group(each group had three repeated holes). Cell proliferation was assessed by cell counting kit- 8 on the 4, 7, 14, and 21 days. The state of adhesion and growth of hDPSC on the materials surface was evaluated by scanning electron microscopy and cytoskeleton staining; and alkaline phosphatase (ALP) activity was measured to evaluate the cell differentiation potential. The expression of odontoblastic differentiation-related genes was measured by real-time PCR.
  
  
  RESULTS
  Surface contact angle of the protein membrane group and composite membrane group A and group B were 89.51° ± 0.12°, 70.32° ± 0.07° and 71.31° ± 0.09° respectively. hDPSC adhered well on each materials surface on the 7, 14, 21 days, ALP activity and differentiation genes of composite membrane group A and B rised more significantly than the blank control group and protein membrane group did (P<0.05). Dentin matrix protein1(DMP- 1), dentin sialoprotein(DSP), ALP, osteocalcin(OC) mRNA expression reached peak on the 14 days in group A, and in group B on the 21 days. Bone sialoprotein(BSP) mRNA expression in both group A and B reached peak on the 21 days.
  
  
  CONCLUSIONS
  BG45S5- SF membrane is able to support the proliferation and showed the potential of odontoblastic differentiation for hDPSC. This finding suggests that BG45S5-SF membrane was a kind of tissue engineering film material with the regeneration potential for pulp-dentine complex.
authors.affiliation: Department of Digital Dental Center, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
authors.forename: Xiaoshuai
authors.initials: X
authors.lastname: Lyu
authors.affiliation: Department of Digital Dental Center, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
authors.forename: Zhengmao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Digital Dental Center, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Digital Dental Center, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China; Email: xyang.gmu@gmail.com.
authors.forename: Xuechao
authors.initials: X
authors.lastname: Yang
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002516:Ceramics / Q000627:therapeutic use*; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005349:Fibroins / Q000627:therapeutic use*; D005898:Glass; D006801:Humans; D008567:Membranes, Artificial; D009804:Odontoblasts / Q000166:cytology; D015675:Osteocalcin / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D005349:Fibroins; D008567:Membranes, Artificial; D015675:Osteocalcin; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human; C425330:bioactive glass 45S5; C482288:fibroin, silkworm