title: Interfaces with Tunable Mechanical and Radiosensitizing Properties.
abstract: We report the fabrication of a composite containing nanostructured GaOOH and Matrigel with tunable radiosensitizing and stiffness properties. Composite characterization was done with microscopy and rheology. The utility of the interface was tested in vitro using fibroblasts. Cell viability and reactive oxygen species assays quantified the effects of radiation dosages and GaOOH concentrations. Fibroblasts' viability decreased with increasing concentration of GaOOH and composite stiffness. During ionizing radiation experiments the presence of the scintillating GaOOH triggered a different cellular response. Reactive oxygen species data demonstrated that one can reduce the amount of radiation needed to modulate the behavior of cells on interfaces with different stiffness containing a radiosensitizing material.
authors.forename: Nora G
authors.initials: NG
authors.lastname: Berg
authors.forename: Brady L
authors.initials: BL
authors.lastname: Pearce
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Snyder
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Rohrbaugh
authors.affiliation: Department of Clinical Sciences (College of Veterinary Medicine) and Comparative Medicine Institute, North Carolina State University , Raleigh, North Carolina 27606, United States.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Nolan
authors.forename: Prajesh
authors.initials: P
authors.lastname: Adhikari
authors.forename: Saad A
authors.initials: SA
authors.lastname: Khan
authors.forename: Albena
authors.initials: A
authors.lastname: Ivanisevic
mesh_terms: D002470:Cell Survival; D005347:Fibroblasts; D049329:Nanostructures; D017382:Reactive Oxygen Species
chemical_list: D017382:Reactive Oxygen Species
keywords: GaOOH; NIH 3T3 Cells; X-ray radiation; extracellular matrix composite; mechanobiology; nanocomposite