title: [Anti-oncogene of opioid binding protein/cell adhesion molecule-like methylation status in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate].
abstract: OBJECTIVE
  To analyze the opioid binding protein/cell adhesion molecule-like (OPCML) methylation status at different stages of malignant transformation of human bronchial epithelial (16HBE) cells induced by Glycidyl Methacrylate (GMA) and to explore the effect of OPCML methylation in the process of malignant transformation.
  
  
  METHODS
  Cells were harvested at different stages (the 10th generation, the 20th generation and the 30th generation). To verify the Methylation chip result of OPCML methylation status in the process of malignant transformation, we detected it by methylation-specific-PCR (MSP); Real-time fluorescence Quantitative PCR (qPCR) were applied to measure the gene expression levels of OPCML at different transformed stage, and compared with the control groups (treated with DMSO).
  
  
  RESULTS
  Based on the result of methylation chip, the gene of OPCML methylation occurred in all stages, which was consistent to the result of MSP; qPCR showed that the levels of gene expression decreased in the 20th generation and 30th generation.
  
  
  CONCLUSIONS
  Methylation status of OPCML gene promoter could be considered as a stable and specific biomarker in the transformation process.
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Liu
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Quankai
authors.initials: Q
authors.lastname: Wang
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Guangyun
authors.initials: G
authors.lastname: Xie
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Li
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Anna
authors.initials: A
authors.lastname: Wang
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wen
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China; E-mail: jnx999@263.net.
authors.forename: Jianning
authors.initials: J
authors.lastname: Xu
mesh_terms: D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D019175:DNA Methylation; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D004852:Epoxy Compounds / Q000009:adverse effects*; D058851:GPI-Linked Proteins / Q000378:metabolism; D016147:Genes, Tumor Suppressor; D006801:Humans; D008689:Methacrylates / Q000009:adverse effects*; D011401:Promoter Regions, Genetic
chemical_list: D015815:Cell Adhesion Molecules; D004852:Epoxy Compounds; D058851:GPI-Linked Proteins; D008689:Methacrylates; C059785:OPCML protein, human; C007870:glycidyl methacrylate