title: Water-soluble poly(2,7-dibenzosilole) as an ultra-bright fluorescent label for antibody-based flow cytometry.
abstract: A series of novel water-soluble PEGylated dibenzosilole-based conjugated polymers were prepared as ultra-bright fluorescent labels for biomolecules. Due to their superior solubility and brightness, antibody conjugates labeled with functionalized polymers showed significantly enhanced signal and sensitivity relative to traditional fluorophores in functional flow cytometry applications.
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Yi-Zhen
authors.initials: YZ
authors.lastname: Hu
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Aimei
authors.initials: A
authors.lastname: Chen
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Yexin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Robert
authors.initials: R
authors.lastname: Aggeler
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Low
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Hee Chol
authors.initials: HC
authors.lastname: Kang
authors.affiliation: Biosciences Division, Thermo Fisher Scientific Inc. 29851 Willow Creek Rd., Eugene, OR 97402, USA. Kyle.Gee@thermofisher.com.
authors.forename: Kyle R
authors.initials: KR
authors.lastname: Gee
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D017646:Organosilicon Compounds; D011108:Polymers; C000610540:poly(2,7-dibenzosilole); D014867:Water; D011092:Polyethylene Glycols