title: Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering.
abstract: The N protein of coronaviruses is a multifunctional protein that is organized into several domains. The N-terminal part is composed of an intrinsically disordered region (IDR) followed by a structured domain called the N-terminal domain (NTD). In this study, the structure determination of the N-terminal region of the MERS-CoV N protein via X-ray diffraction measurements is reported at a resolution of 2.4 Å. Since the first 30 amino acids were not resolved by X-ray diffraction, the structural study was completed by a SAXS experiment to propose a structural model including the IDR. This model presents the N-terminal region of the MERS-CoV as a monomer that displays structural features in common with other coronavirus NTDs.
authors.affiliation: CNRS, AFMB UMR 7257, 13288 Marseille, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papageorgiou
authors.affiliation: CNRS, AFMB UMR 7257, 13288 Marseille, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Lichière
authors.affiliation: CNRS, AFMB UMR 7257, 13288 Marseille, France.
authors.forename: Amal
authors.initials: A
authors.lastname: Baklouti
authors.affiliation: CNRS, AFMB UMR 7257, 13288 Marseille, France.
authors.forename: François
authors.initials: F
authors.lastname: Ferron
authors.affiliation: CNRS, AFMB UMR 7257, 13288 Marseille, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Sévajol
authors.affiliation: CNRS, AFMB UMR 7257, 13288 Marseille, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Canard
authors.affiliation: CNRS, AFMB UMR 7257, 13288 Marseille, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Coutard
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D065207:Middle East Respiratory Syndrome Coronavirus / Q000737:chemistry; D008958:Models, Molecular; D019590:Nucleocapsid Proteins / Q000737:chemistry*; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D053838:Scattering, Small Angle
chemical_list: D019590:Nucleocapsid Proteins
keywords: MERS-CoV; RNA-binding domain; SAXS; nucleocapsid; structure