title: Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive.
abstract: Enantiomeric separation of citalopram (CIT) was developed using a reversed phase HPLC (RP-HPLC) with sulfobutylether-β-cyclodextrin (SBE-β-CD) as a chiral mobile phase additive. The effects of the pH value of aqueous buffer, concentration of chiral additive, composition of mobile phase, and column temperature on the enantioseparation of CIT were investigated on the Hedera ODS-2 C18 column (250 mm × 4.6 mm × 5.0 um). A satisfactory resolution was achieved at 25°C using a mobile phase consisting of a mixture of aqueous buffer (pH of 2.5, 5 mM sodium dihydrogen phosphate, and 12 mM SBE-β-CD), methanol, and acetonitrile with a volumetric ratio of 21 : 3 : 1 and flow rate of 1.0 mL/min. This analytical method was evaluated by examining the precision (lower than 3.0%), linearity (regression coefficients close to 1), limit of detection (0.070 µg/mL for (R)-CIT and 0.076 µg/mL for (S)-CIT), and limit of quantitation (0.235 µg/mL for (R)-CIT and 0.254 µg/mL for (S)-CIT).
authors.affiliation: School of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yangfeng
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Engineering, Faculty of Agriculture, Dalhousie University, Truro, NS, Canada B2N 5E3.
authors.forename: Quan Sophia
authors.initials: QS
authors.lastname: He
authors.affiliation: School of Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Cai