title: AMPK is involved in the differential neonatal performance of chicks hatching at different time.
abstract: We have recently reported that the hatching time may be in relation to the distinct neonatal performance of female chicks. The present study was aimed to investigate the potential involvement of AMPK, an energy sensor which plays a pivotal role in energy homeostasis, in the distinct performance of the spread of hatching time model. As a result, hypothalamic AMPKα1 isoform gene expression was significantly higher in the late hatcher as compared to that of their early counterparts, whereas the total and phosphorylated levels of AMPKα subunit did not differ between the three hatchers. The hypothalamic orexigenic NPY and AgRP mRNA levels were higher in the late hatchers as compared to the early, and that of the middle hatchers was at an intermediate level. However, the anorexigenic POMC and CRH was also higher expressed in the late hatchers as compared to the early hatchers. In the liver, AMPKα2 mRNA level and the phosphorylation ratio of AMPKα was significantly lower in the late hatchers, as compared to their early counterparts. The hepatic phosphorylated GS levels of the late and middle hatchers were lower than that of their early counterparts. The expression of hepatic FTO gene of the late hatchers was significantly higher than that of their early and middle counterparts. Taken together, AMPK may play a significant role in the different neonatal performance of the spread of hatching time model. The central and peripheral AMPK in late hatchers exhibited a pattern of higher energy intake and lower energy expenditure, which resulted in a faster post-hatch growth.
authors.affiliation: Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven. Kasteelpark Arenberg 30, 3001 Leuven, Belgium.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven. Kasteelpark Arenberg 30, 3001 Leuven, Belgium. Electronic address: johan.buyse@biw.kuleuven.be.
authors.forename: Johan
authors.initials: J
authors.lastname: Buyse
authors.affiliation: Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Song
authors.affiliation: Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven. Kasteelpark Arenberg 30, 3001 Leuven, Belgium.
authors.forename: Eddy
authors.initials: E
authors.lastname: Decuypere
authors.affiliation: Laboratory of Livestock Physiology, Department of Biosystems, KU Leuven. Kasteelpark Arenberg 30, 3001 Leuven, Belgium; Animal Science Unit, University of Liège, Gembloux Agro-Bio Tech, Passage des Déportés 2, 5030 Gembloux, Belgium.
authors.forename: Nadia
authors.initials: N
authors.lastname: Everaert
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D054369:Agouti-Related Protein / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002645:Chickens / Q000254:growth & development* / Q000378:metabolism; D004734:Energy Metabolism; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D054369:Agouti-Related Protein; D009478:Neuropeptide Y; D012333:RNA, Messenger; D055372:AMP-Activated Protein Kinases
keywords: AMPK; Broiler chicks; Glycogen synthase; Hatching time; Neuropeptides