title: The role of in-vivo and in-vitro maturation time on ooplasmic dysmaturity.
abstract: This study investigates whether the timing of in-vivo and in-vitro maturation influences ooplasmic dysmaturity. This is a retrospective comparison of intracytoplasmic sperm injection (ICSI) cycles (index cycles) complicated by complete fertilization failure (CFF) to cycles with successful fertilization in the same patient. The cycle following the index cycle was modified intentionally to increase fertilization. The times between human chorionic gonadotrophin (HCG) trigger and oocyte retrieval, HCG trigger and removal of cumulus cells, and HCG trigger and sperm injection were recorded. Fifteen patients were included. Compared with successful fertilization cycles, index (CFF) cycles showed a shorter time interval between HCG trigger and oocyte retrieval (2029.0 ± 16 versus 2195.0 ± 10 min; P < 0.001), HCG trigger and removal of cumulus cells (2201.4 ± 15 versus 2309.0 ± 23 min; P < 0.001) and oocyte retrieval and removal of cumulus cells (114.0 ± 13 versus 171.8 ± 15 min; P < 0.001). The interval between HCG trigger and ICSI was comparable between groups. Findings reveal novel patterns in time intervals between HCG trigger, oocyte retrieval, removal of cumulus cells and ICSI. Thus, modulating time intervals between HCG trigger, oocyte retrieval, removal of cumulus cells and ICSI to grant fertilization seems feasible.
authors.affiliation: Ronald O Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Nigel
authors.initials: N
authors.lastname: Pereira
authors.affiliation: Ronald O Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Queenie V
authors.initials: QV
authors.lastname: Neri
authors.affiliation: Ronald O Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Jovana P
authors.initials: JP
authors.lastname: Lekovich
authors.affiliation: Ronald O Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York, USA. Electronic address: gdpalerm@med.cornell.edu.
authors.forename: Gianpiero D
authors.initials: GD
authors.lastname: Palermo
authors.affiliation: Ronald O Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Zev
authors.initials: Z
authors.lastname: Rosenwaks
mesh_terms: D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005306:Fertilization; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000254:growth & development*; D010062:Ovulation Induction; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin
keywords: Fertilization failure; ICSI; nuclear/cytoplasmic asynchrony; oocyte maturity; ooplasmic maturity