title: Preventive and therapeutic effects of blueberry (Vaccinium corymbosum) extract against DSS-induced ulcerative colitis by regulation of antioxidant and inflammatory mediators.
abstract: Inflammatory bowel disease (IBD) is an inflammatory disorder caused by hyperactivation of effector immune cells that produce high levels of proinflammatory cytokines. The aims of our study were to determine whether orally administered blueberry extract (BE) could attenuate or prevent the development of experimental colitis in mice and to elucidate the mechanism of action. Female Balb/C mice (n=7) were randomized into groups differing in treatment conditions (prevention and treatment) and dose of BE (50 mg/kg body weight). Acute ulcerative colitis was induced by oral administration of 3% dextran sodium sulfate for 7 days in drinking water. Colonic mucosal injury was assessed by clinical, macroscopic, biochemical and histopathological examinations. BE significantly decreased disease activity index and improved the macroscopic and histological score of colons when compared to the colitis group (P<.05). BE markedly attenuated myeloperoxidase accumulation (colitis group 54.97±2.78 nmol/mg, treatment group 30.78±1.33 nmol/mg) and malondialdehyde in colon and prostaglandin E2 level in serum while increasing the levels of superoxide dismutase and catalase (colitis group 11.94±1.16 U/ml, BE treatment group 16.49±0.39 U/ml) compared with the colitis group (P<.05). mRNA levels of the cyclooxygenase (COX)-2, interferon-γ, interleukin (IL)-1β and inducible nitric oxide synthase cytokines were determined by reverse transcriptase polymerase chain reaction. Immunohistochemical analysis showed that BE attenuates the expression of COX-2 and IL-1β in colonic tissue. Moreover, BE reduced the nuclear translocation of nuclear transcription factor kappa B (NF-κB) by immunofluorescence analysis. Thus, the anti-inflammatory effect of BE at colorectal sites is a result of a number of mechanisms: antioxidation, down-regulation of the expression of inflammatory mediators and inhibition of the nuclear translocation of NF-κB.
authors.affiliation: College of Biomedical & Health Science, Department of Life Science, Konkuk University, Chungju, 380-701, Republic of Korea.
authors.forename: Mehnaz
authors.initials: M
authors.lastname: Pervin
authors.affiliation: College of Biomedical & Health Science, Department of Life Science, Konkuk University, Chungju, 380-701, Republic of Korea.
authors.forename: Md Abul
authors.initials: MA
authors.lastname: Hasnat
authors.affiliation: College of Biomedical & Health Science, Department of Life Science, Konkuk University, Chungju, 380-701, Republic of Korea.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Lim
authors.affiliation: College of Biomedical & Health Science, Department of Life Science, Konkuk University, Chungju, 380-701, Republic of Korea.
authors.forename: Yoon-Mi
authors.initials: YM
authors.lastname: Lee
authors.affiliation: Functional Food Center, KIST Gangneung Institute, 290, Daejeon-dong, Gangneung, Gangwon, 210-340, Republic of Korea.
authors.forename: Eun Ok
authors.initials: EO
authors.lastname: Kim
authors.affiliation: Functional Food Center, KIST Gangneung Institute, 290, Daejeon-dong, Gangneung, Gangwon, 210-340, Republic of Korea.
authors.forename: Byung-Hun
authors.initials: BH
authors.lastname: Um
authors.affiliation: College of Biomedical & Health Science, Department of Life Science, Konkuk University, Chungju, 380-701, Republic of Korea. Electronic address: beongou@kku.ac.kr.
authors.forename: Beong Ou
authors.initials: BO
authors.lastname: Lim
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D029798:Blueberry Plants / Q000737:chemistry*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D016264:Dextran Sulfate / Q000633:toxicity*; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism*; D051379:Mice; D010936:Plant Extracts / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D018836:Inflammation Mediators; D010936:Plant Extracts; D016264:Dextran Sulfate
keywords: Blueberry extract; Immunofluorescence; Immunohistochemistry; Inflammatory bowel Disease; NF-κB