title: Detection of Matrilysin Activity Using Polypeptide Functionalized Reduced Graphene Oxide Field-Effect Transistor Sensor.
abstract: A novel approach for rapid and sensitive detection of matrilysin (MMP-7, a biomarker involved in the degradation of various macromolecules) based on a polypeptide (JR2EC) functionalized reduced graphene oxide (rGO) field effect transistor (FET) is reported. MMP-7 specifically digests negatively charged JR2EC immobilized on rGO, thereby modulating the conductance of rGO-FET. The proposed assay enabled detection of MMP-7 at clinically relevant concentrations with a limit of detection (LOD) of 10 ng/mL (400 pM), attributed to the significant reduction of the net charge of JR2EC upon digestion by MMP-7. Quantitative detection of MMP-7 in human plasma was further demonstrated with a LOD of 40 ng/mL, illustrating the potential for the proposed methodology for tumor detection and carcinoma diagnostic (e.g., lung cancer and salivary gland cancer). Additionally, excellent specificity of the proposed assay was demonstrated using matrix metallopeptidase 1 (MMP-1), a protease of the same family. With appropriate selection and modification of polypeptides, the proposed assay could be extended for detection of other enzymes with polypeptide digestion capability.
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.|Institute for Sports Research, Nanyang Technological University , 50 Nanyang Avenue, Singapore 639798.|Department of Chemistry, Loughborough University , Leicestershire LE11 3TU, U.K.
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.|Centre for Biomimetic Sensor Science , 50 Nanyang Drive, Singapore 637553.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.|Institute for Sports Research, Nanyang Technological University , 50 Nanyang Avenue, Singapore 639798.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Huang
authors.affiliation: Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University , SE-581 83, Sweden.
authors.forename: Robert
authors.initials: R
authors.lastname: Selegård
authors.affiliation: Department of Chemistry, Loughborough University , Leicestershire LE11 3TU, U.K.
authors.forename: Mark
authors.initials: M
authors.lastname: Platt
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.|Centre for Biomimetic Sensor Science , 50 Nanyang Drive, Singapore 637553.
authors.forename: Alagappan
authors.initials: A
authors.lastname: Palaniappan
authors.affiliation: Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University , SE-581 83, Sweden.
authors.forename: Daniel
authors.initials: D
authors.lastname: Aili
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.|Institute for Sports Research, Nanyang Technological University , 50 Nanyang Avenue, Singapore 639798.
authors.forename: Alfred Iing Yoong
authors.initials: AI
authors.lastname: Tok
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University , Singapore 639798.|Centre for Biomimetic Sensor Science , 50 Nanyang Drive, Singapore 637553.
authors.forename: Bo
authors.initials: B
authors.lastname: Liedberg
mesh_terms: D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*
chemical_list: D010087:Oxides; D010455:Peptides; D006108:Graphite; D020783:Matrix Metalloproteinase 7