title: High-salt diets during pregnancy increases renal vascular reactivity due to altered soluble guanylyl cyclase-related pathways in rat offspring.
abstract: Adverse prenatal factors such as overtake of salt or fat food are potential risks for cardiovascular diseases in offspring. This study tested the hypothesis that prenatal high-salt (HS) diets may influence renal vascular tone and attenuates signaling pathways related to soluble guanylyl cyclase (sGC) or/and large-conductance Ca(2+)-activated K(+) (BKCa) channels in the offspring. Pregnant rats were fed either normal salt (NS) (1% NaCl) or HS (8% NaCl) diet for the whole gestation. Offspring were maintained on NS diets. Renal interlobar arteries in offspring were tested for vascular responses to phenylephrine (Phe), K(+) channels and signal pathways related to sGC. Phe induced higher vessel tension in interlobar arteries of the HS offspring. Following pretreatment with BKCa channel inhibitor iberiotoxin, Phe-mediated vasoconstrictions were decreased in HS offspring compared to NS. Phe-mediated constrictions following pretreatment with NO synthase inhibitor N(G)-nitro-l-arginine methyl ester or sGC inhibitor 1H-1,2,4-oxadiazolo-4,3-quinoxalin-1-one in the HS offspring were less sensitive than NS. The whole-cell K(+) currents and the component of BKCa channels were not changed in smooth muscle cells from interlobar arteries, whereas the K(+) currents stimulated by sGC activator BAY41-2272 were reduced in the HS offspring. The protein expressions of sGC β1 and β2 in the interlobar arteries of HS offspring were reduced. The results showed that chronic overintake of salt during pregnancy could increase renal vascular tone in the offspring. The affected signal pathways included down-regulation of sGC function and expression.
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yin
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Axin
authors.initials: A
authors.lastname: He
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Le
authors.initials: L
authors.lastname: Bo
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Tang
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Gu
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Juanxiu
authors.initials: J
authors.lastname: Lv
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Mao
authors.affiliation: Institute for Fetology and Reproductive Medicine Center, First Hospital of Soochow University, Suzhou 215006, China; Center for Prenatal Biology, Loma Linda University, CA 92350, USA. Electronic address: xuzhice@suda.edu.cn.
authors.forename: Zhice
authors.initials: Z
authors.lastname: Xu
mesh_terms: D000818:Animals; D005260:Female; D006162:Guanylate Cyclase / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply*; D011247:Pregnancy; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage*; D000071756:Soluble Guanylyl Cyclase
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear; D017673:Sodium Chloride, Dietary; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase
keywords: BK(Ca) channels; PKG; Prenatal HS; Renal interlobar arteries; sGC