title: Recombinant infectious bronchitis virus (IBV) H120 vaccine strain expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) protects chickens against IBV and NDV challenge.
abstract: Infectious bronchitis (IB) and Newcastle disease (ND) are common viral diseases of chickens, which are caused by infectious bronchitis virus (IBV) and Newcastle disease virus (NDV), respectively. Vaccination with live attenuated strains of IBV-H120 and NDV-LaSota are important for the control of IB and ND. However, conventional live attenuated vaccines are expensive and result in the inability to differentiate between infected and vaccinated chickens. Therefore, there is an urgent need to develop new efficacious vaccines. In this study, using a previously established reverse genetics system, we generated a recombinant IBV virus based on the IBV H120 vaccine strain expressing the haemagglutinin-neuraminidase (HN) protein of NDV. The recombinant virus, R-H120-HN/5a, exhibited growth dynamics, pathogenicity and viral titers that were similar to those of the parental IBV H120, but it had acquired hemagglutination activity from NDV. Vaccination of SPF chickens with the R-H120-HN/5a virus induced a humoral response at a level comparable to that of the LaSota/H120 commercial bivalent vaccine and provided significant protection against challenge with virulent IBV and NDV. In summary, the results of this study indicate that the IBV H120 strain could serve as an effective tool for designing vaccines against IB and other infectious diseases, and the generation of IBV R-H120-HN/5a provides a solid foundation for the development of an effective bivalent vaccine against IBV and NDV.
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Yingshun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Li
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Gaosheng
authors.initials: G
authors.lastname: Ji
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Fanya
authors.initials: F
authors.lastname: Zeng
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Gao
authors.affiliation: Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, School of Life Science, Sichuan University, Chengdu, 610064, China. whongning@163.com.
authors.forename: Hongning
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology; D001351:Infectious bronchitis virus / Q000276:immunology*; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D017778:Vaccines, Combined / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D006388:Hemagglutinins; D017778:Vaccines, Combined; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D009439:Neuraminidase