title: Pathogens in Ornamental Waters: A Pilot Study.
abstract: In parks, ornamental waters of easy access and populated with animals are quite attractive to children and yet might hide threats to human health. The present work focuses on the microbiota of the ornamental waters of a Lisboa park, characterized during 2015. The results show a dynamic microbiota integrating human pathogens such as Klebsiella pneumoniae, Aeromonas spp. and Enterobacter spp., and also antibiotic resistant bacteria. K. pneumoniae and Aeromonas spp. were present as planktonic and biofilm organized bacteria. In vitro K. pneumoniae and Aeromonas spp. showed an enhanced ability to assemble biofilm at 25 °C than at 37 °C. Bacteria recovered from biofilm samples showed an increased antibiotic resistance compared to the respective planktonic counterparts.
authors.affiliation: Department of Environmental Health (DSA), National Institute of Health Dr Ricardo Jorge (INSA), Avenida Padre Cruz, Lisboa 1649-016, Portugal. maria_d_n@hotmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Nascimento
authors.affiliation: Department of Infectious Diseases (DDI), INSA, Avenida Padre Cruz, Lisboa 1649-016, Portugal. joao.rodrigues@insa.min-saude.pt.
authors.forename: Joao Carlos
authors.initials: JC
authors.lastname: Rodrigues
authors.affiliation: Department of Infectious Diseases (DDI), INSA, Avenida Padre Cruz, Lisboa 1649-016, Portugal. lucia.reis@insa.min-saude.pt.
authors.forename: Lucia
authors.initials: L
authors.lastname: Reis
authors.affiliation: Departament of Chemical Engineering, Instituto Superior Técnico, University of Lisboa (UL), Av Rovisco Pais, Lisboa 1049-001, Portugal. isabel.nogueira@ist.utl.pt.
authors.forename: Isabel
authors.initials: I
authors.lastname: Nogueira
authors.affiliation: Departament of Chemical Engineering, Instituto Superior Técnico, University of Lisboa (UL), Av Rovisco Pais, Lisboa 1049-001, Portugal. pac@ist.utl.pt.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Carvalho
authors.affiliation: Department of Environmental Health (DSA), National Institute of Health Dr Ricardo Jorge (INSA), Avenida Padre Cruz, Lisboa 1649-016, Portugal. joao.brandao@insa.min-saude.pt.
authors.forename: João
authors.initials: J
authors.lastname: Brandão
authors.affiliation: Faculty of Farmacy, Departament of Microbiology and Imunology; iMed.UL, UL, Av Prof Gama Pinto, Lisboa 1649-003, Portugal. aduarte@ff.ul.pt.
authors.forename: Aida
authors.initials: A
authors.lastname: Duarte
authors.affiliation: Department of Environmental Health (DSA), National Institute of Health Dr Ricardo Jorge (INSA), Avenida Padre Cruz, Lisboa 1649-016, Portugal. maria.jordao@insa.min-saude.pt.
authors.forename: Luisa
authors.initials: L
authors.lastname: Jordao
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms; D024881:Drug Resistance, Bacterial; D004784:Environmental Monitoring; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D064307:Microbiota / Q000187:drug effects; D000068316:Parks, Recreational; D010865:Pilot Projects; D011174:Portugal; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: biofilms; children’s health; environmental exposures; play behavior; waterborne pathogens