title: Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation.
abstract: To improve the biomass yield and lipid productivity, two desert microalgae, Desmodesmus sp. S81 and G41 were induced mutagenesis by Ethylmethane sulfonate (EMS), and obtained two potential mutants, Desmodesmus sp. S5 and G3 from the mutagenic clones for their greatly promoted biomass and lipid production. The results showed that the biomass yield, lipid content and lipid productivity of the mutant strains S5 and G3 were 778.10mg·L(-1), 48.41% and 19.83mg·L(-1)·d(-1), 739.52mg·L(-1), 46.01%, and 17.92mg·L(-1)·d(-1), respectively, which presented the increment of 45.50%, 8.00% and 74.24%, 20.67%, 10.35% and 55.77% than those of S81 and G41. Comparing with the wild strains, the mutants showed reduced PUFAs and glycol lipids, elevated MUFAs and neutral lipids contents, which were appropriate for biodiesel production.
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Meilin
authors.initials: M
authors.lastname: He
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shanmei
authors.initials: S
authors.lastname: Zou
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Fei
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Aftab Ahmed
authors.initials: AA
authors.lastname: Rajper
authors.affiliation: Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: chwang@njau.edu.cn.
authors.forename: Changhai
authors.initials: C
authors.lastname: Wang
mesh_terms: D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D002734:Chlorophyll / Q000378:metabolism; D000460:Chlorophyta / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005453:Fluorescence; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics*; D010078:Oxazines / Q000378:metabolism; D013997:Time Factors
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D010078:Oxazines; D002734:Chlorophyll; D005020:Ethyl Methanesulfonate; C044808:nile red
keywords: Biomass; Desmodesmus; Ethylmethane sulfonate; Lipid; Mutation