title: Flow cytometric characterization of microglia in the offspring of PolyI:C treated mice.
abstract: The neuropathology of schizophrenia has been reported to be closely associated with microglial activation. In a previous study, using the prenatal PolyI:C schizophrenia animal model, we showed an increase in cell numbers and a reduction in microglial branching in 30-day-old PolyI:C descendants, which suggests that there is microglial activation during adolescence. To provide more information about the activation state, we aimed to examine the expression levels of Iba1, which was reported to be up-regulated in activated microglia. We used a flow cytometric approach and investigated CD11b and CD45, two additional markers for the identification of microglial cells. We demonstrated that intracellular staining against Iba1 can be used as a reliable flow cytometric method for identification of microglial cells. Prenatal PolyI:C treatment had long-term effects on CD11b and CD45 expression. It also resulted in a trend towards increased Iba1 expression. Imbalance in CD11b, CD45, and Iba1 expression might contribute to impaired synaptic surveillance and enhanced activation/inflammatory activity of microglia in adult offspring.
authors.affiliation: Department of Psychiatry, LWL University Hospital, Ruhr-University Bochum, Alexandrinenstr. 1, D-44791 Bochum, Germany. Electronic address: marie-pierre.manitz@rub.de.
authors.forename: Marie Pierre
authors.initials: MP
authors.lastname: Manitz
authors.affiliation: Department of Psychiatry, LWL University Hospital, Ruhr-University Bochum, Alexandrinenstr. 1, D-44791 Bochum, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Plümper
authors.affiliation: Department of Neuroimmunology, Ruhr-University Bochum, Universitätsstr. 150, D-44801 Bochum, Germany. Electronic address: seray.demir@rub.de.
authors.forename: Seray
authors.initials: S
authors.lastname: Demir
authors.affiliation: Medizinisches Proteom-Center, Ruhr-University Bochum, Universitätsstr. 150, D-44801 Bochum, Germany. Electronic address: maike.ahrens@rub.de.
authors.forename: Maike
authors.initials: M
authors.lastname: Ahrens
authors.affiliation: Department of Psychiatry, LWL University Hospital, Ruhr-University Bochum, Alexandrinenstr. 1, D-44791 Bochum, Germany. Electronic address: manuela.esslinger@rub.de.
authors.forename: Manuela
authors.initials: M
authors.lastname: Eßlinger
authors.affiliation: Department of Psychiatry, LWL University Hospital, Ruhr-University Bochum, Alexandrinenstr. 1, D-44791 Bochum, Germany. Electronic address: simone.wachholz@rub.de.
authors.forename: Simone
authors.initials: S
authors.lastname: Wachholz
authors.affiliation: Medizinisches Proteom-Center, Ruhr-University Bochum, Universitätsstr. 150, D-44801 Bochum, Germany. Electronic address: martin.eisenacher@rub.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Eisenacher
authors.affiliation: Department of Psychiatry, LWL University Hospital, Ruhr-University Bochum, Alexandrinenstr. 1, D-44791 Bochum, Germany. Electronic address: georg.juckel@wkp-lwl.org.
authors.forename: Georg
authors.initials: G
authors.lastname: Juckel
authors.affiliation: Department of Psychiatry, LWL University Hospital, Ruhr-University Bochum, Alexandrinenstr. 1, D-44791 Bochum, Germany. Electronic address: astrid.friebe@rub.de.
authors.forename: Astrid
authors.initials: A
authors.lastname: Friebe
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects; D039481:CD11b Antigen / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D007369:Interferon Inducers / Q000633:toxicity*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism; D017628:Microglia / Q000473:pathology*; D011070:Poly I-C / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000473:pathology*; D012727:Sex Characteristics
chemical_list: C491049:Aif1 protein, mouse; D039481:CD11b Antigen; D002135:Calcium-Binding Proteins; D007369:Interferon Inducers; D008840:Microfilament Proteins; D017493:Leukocyte Common Antigens; D011070:Poly I-C
keywords: CD11b; CD45; Cellular activation; Flow cytometry; Iba1; Microglia; Morphology; PolyI:C