title: Polypropylene Sulfide Nanoparticle p24 Vaccine Promotes Dendritic Cell-Mediated Specific Immune Responses against HIV-1.
abstract: Delivery of vaccine formulations into the dermis using antigen-coated microneedle patches is a promising and safe approach because of efficient antigen delivery and safety. We evaluated an intradermal vaccine using HIV-1 p24 Gag peptide-conjugated polypropylene sulfide nanoparticles to induce immunity against HIV-1. This peptide-conjugated polypropylene sulfide nanoparticle formulation did not accelerate the maturation of blood- or skin-derived subsets of dendritic cells, either generated in vitro or purified ex vivo, despite efficient uptake in the absence of adjuvant. Moreover, dendritic cell-mediated capture of particulate antigen in this form induced potent HIV-1-specific CD4(+) T-cell responses, as well as B-cell-mediated antibody production. Nanoparticle-based intradermal antigen delivery may therefore provide a new option in the global effort to develop an effective vaccine against HIV-1.
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Caucheteux
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: John P
authors.initials: JP
authors.lastname: Mitchell
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK; School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK.
authors.forename: Matthew O
authors.initials: MO
authors.lastname: Ivory
authors.affiliation: Institute of Bioengineering, School of Life Sciences and School of Engineering, Lausanne, Switzerland.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hirosue
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Hakobyan
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: Garry
authors.initials: G
authors.lastname: Dolton
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: Kristin
authors.initials: K
authors.lastname: Ladell
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: Kelly
authors.initials: K
authors.lastname: Miners
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Price
authors.affiliation: Department of Biological Sciences, University of Texas at El Paso, El Paso, Texas, USA.
authors.forename: June
authors.initials: J
authors.lastname: Kan-Mitchell
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Sewell
authors.affiliation: St. John's Institute of Dermatology, King's College London, London, UK.
authors.forename: Frank
authors.initials: F
authors.lastname: Nestle
authors.affiliation: Sorbonne Universités, UPMC Paris 06, INSERM U1135, CNRS ERL 8255, Center for Immunology and Microbial Infections, F-75013, Paris, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Moris
authors.affiliation: Spire Cardiff Hospital, Cardiff, UK.
authors.forename: Richard O
authors.initials: RO
authors.lastname: Karoo
authors.affiliation: School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK.
authors.forename: James C
authors.initials: JC
authors.lastname: Birchall
authors.affiliation: Institute of Bioengineering, School of Life Sciences and School of Engineering, Lausanne, Switzerland; Institute for Molecular Engineering, University of Chicago, Chicago, Illinois, USA.
authors.forename: Melody A
authors.initials: MA
authors.lastname: Swartz
authors.affiliation: Institute of Bioengineering, School of Life Sciences and School of Engineering, Lausanne, Switzerland; Institute for Molecular Engineering, University of Chicago, Chicago, Illinois, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Hubbel
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: Fabien P
authors.initials: FP
authors.lastname: Blanchet
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK. Electronic address: piguetv@cardiff.ac.uk.
authors.forename: Vincent
authors.initials: V
authors.lastname: Piguet
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D016503:Drug Delivery Systems / Q000379:methods*; D015658:HIV Infections / Q000517:prevention & control; D015497:HIV-1 / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D053758:Nanoparticles / Q000008:administration & dosage; D011126:Polypropylenes / Q000494:pharmacology; D013440:Sulfides / Q000494:pharmacology; D014612:Vaccines / Q000008:administration & dosage*
chemical_list: D011126:Polypropylenes; D013440:Sulfides; D014612:Vaccines