title: Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from Human Endometrium.
abstract: Cell cultures isolated from human endometrium by enzyme digestion consisted of highly viable fibroblast-like mesenchymal cells expressing CD90, CD73, and CD105. During passage 1, the cultures contained a small fraction of cytokeratin-7(+) epithelial cells that disappeared by passage 2. The cultures from the endometrium could be induced to adipogenic, osteogenic and chondrogenic differentiation in vitro. These findings suggest that human endometrium is a convenient source of biomaterial for minimally invasive isolation of cultures that exhibit typical morphology and immunophenotypic profile of resident multipotent mesenchymal stromal cells retain high viability in vitro.
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. bushueva@gmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Savilova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Yushina
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: Y V
authors.initials: YV
authors.lastname: Rudimova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E G
authors.initials: EG
authors.lastname: Khil'kevich
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V D
authors.initials: VD
authors.lastname: Chuprynin
mesh_terms: D015720:5'-Nucleotidase / Q000096:biosynthesis*; D050156:Adipogenesis / Q000502:physiology; D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology; D000071063:Endoglin / Q000096:biosynthesis*; D004717:Endometrium / Q000166:cytology*; D005260:Female; D058851:GPI-Linked Proteins / Q000096:biosynthesis; D006801:Humans; D053552:Keratin-7 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology; D018800:Thy-1 Antigens / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: C064206:ENG protein, human; D000071063:Endoglin; D058851:GPI-Linked Proteins; D053552:Keratin-7; D018800:Thy-1 Antigens; D015720:5'-Nucleotidase; C548241:NT5E protein, human
keywords: endometrium; immunophenotype; multipotent mesenchymal stromal cells; viability