title: Role of the 52 KDa thioredoxin protein disulfide isomerase of Toxoplasma gondii during infection to human cells.
abstract: Toxoplasma protein disulfide isomerase (PDI) is a 52 KDa thioredoxin of interest because have a great immunogenicity for humans. We cloned and produced a recombinant protein (recTgPDI) used to test its effect during infection to different human cell lines (epithelial and retinal). We also determine if there were differences in gen expression during in vitro infection. Expression of the gen was lower after entry into the host cells. PDI's inhibitors bacitracin and nitroblue tetrazolium reduced the percent of infected cells and small amounts of recTgPDI proteins interfered with the invasion step. All these results support a role of Toxoplasma PDI during the first steps of infection (adhesion and invasion). Toxoplasma PDI is a protein linked to early steps of invasion, it would be of importance to identify the host proteins substrates during invasion steps.
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia.
authors.forename: Diego
authors.initials: D
authors.lastname: Moncada
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia.
authors.forename: Aylan
authors.initials: A
authors.lastname: Arenas
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Acosta
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia.
authors.forename: Diego
authors.initials: D
authors.lastname: Molina
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Hernández
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia.
authors.forename: Néstor
authors.initials: N
authors.lastname: Cardona
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia.
authors.forename: Mónica
authors.initials: M
authors.lastname: Gomez-Yepes
authors.affiliation: Grupo GEPAMOL, Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia, Colombia. Electronic address: gepamol2@uniquindio.edu.co.
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Gomez-Marin
mesh_terms: D002460:Cell Line; D003001:Cloning, Molecular; D063928:Ependymoglial Cells / Q000469:parasitology; D005347:Fibroblasts / Q000469:parasitology; D015971:Gene Expression Regulation, Enzymologic; D006367:HeLa Cells / Q000469:parasitology; D006801:Humans; D008961:Models, Structural; D011487:Protein Conformation; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D013879:Thioredoxins / Q000378:metabolism*; D014122:Toxoplasma / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*
chemical_list: D011994:Recombinant Proteins; D013879:Thioredoxins; D019704:Protein Disulfide-Isomerases
keywords: Protein disulfide isomerase; Thioredoxins; Toxoplasma