title: Design of pH-Responsive Biomaterials to Enable the Oral Route of Hematological Factor IX.
abstract: The oral administration of hematological factor IX (FIX) can offer a convenient prophylactic treatment for hemophilia B patients. pH-Responsive hydrogels based on poly(methacrylic acid)-grafted-poly(ethylene glycol) (P(MAA-g-EG)) have been engineered as delivery vehicles for FIX. In oral delivery, such hydrogel carriers protected FIX from the gastric environment and released it under intestinal conditions as demonstrated by evaluation of the loading and release of FIX. Tailoring of the hydrogel networks improved the loading of FIX within the microcarriers, which is critical for minimizing protein degradation. Optimizing the loading conditions by increasing the incubation time and using a reduced ionic strength buffer further improved the delivery potential of the microcarriers. The presence of the microcarriers significantly enhanced the oral absorption of FIX in vitro. As shown in this work, P(MAA-g-EG) microcarriers are promising candidates for the oral delivery of FIX.
authors.affiliation: McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.|Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, Austin, TX, USA.
authors.forename: Sarena D
authors.initials: SD
authors.lastname: Horava
authors.affiliation: McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.|Institute for Biomaterials, Drug Delivery, and Regenerative Medicine, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.|Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.|Department of Surgery and Perioperative Care, Dell Medical School, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.|Division of Pharmaceutics, College of Pharmacy, The University of Texas at Austin, Austin, TX, USA. peppas@che.utexas.edu.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Peppas
mesh_terms: D000284:Administration, Oral; D016503:Drug Delivery Systems / Q000379:methods*; D005164:Factor IX / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011109:Polymethacrylic Acids / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020100:Hydrogels; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D011092:Polyethylene Glycols; D005164:Factor IX; C000595209:polyethylene glycol 1000
keywords: Biomaterials; Drug delivery; Factor IX; Hydrogels