title: Blood Genome-Wide Transcriptional Profiles of HER2 Negative Breast Cancers Patients.
abstract: Tumors act systemically to sustain cancer progression, affecting the physiological processes in the host and triggering responses in the blood circulating cells. In this study, we explored blood transcriptional patterns of patients with two subtypes of HER2 negative breast cancers, with different prognosis and therapeutic outcome. Peripheral blood samples from seven healthy female donors and 29 women with breast cancer including 14 triple-negative breast cancers and 15 hormone-dependent breast cancers were evaluated by microarray. We also evaluated the stroma in primary tumors. Transcriptional analysis revealed distinct molecular signatures in the blood of HER2- breast cancer patients according to ER/PR status. Our data showed the implication of immune signaling in both breast cancer subtypes with an enrichment of these processes in the blood of TNBC patients. We observed a significant alteration of "chemokine signaling," "IL-8 signaling," and "communication between innate and adaptive immune cells" pathways in the blood of TNBC patients correlated with an increased inflammation and necrosis in their primary tumors. Overall, our data indicate that the presence of triple-negative breast cancer is associated with an enrichment of altered systemic immune-related pathways, suggesting that immunotherapy could possibly be synergistic to the chemotherapy, to improve the clinical outcome of these patients.
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania; Research Center for Functional Genomics, Biomedicine and Translational Medicine, University of Medicine and Pharmacy "I. Hatieganu", 23 Marinescu Street, 400337 Cluj-Napoca, Romania.
authors.forename: Ovidiu
authors.initials: O
authors.lastname: Balacescu
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania; Research Center for Functional Genomics, Biomedicine and Translational Medicine, University of Medicine and Pharmacy "I. Hatieganu", 23 Marinescu Street, 400337 Cluj-Napoca, Romania.
authors.forename: Loredana
authors.initials: L
authors.lastname: Balacescu
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, University of Medicine and Pharmacy "I. Hatieganu", 23 Marinescu Street, 400337 Cluj-Napoca, Romania.
authors.forename: Oana
authors.identifier: 0000-0002-0970-9521
authors.initials: O
authors.lastname: Virtic
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Simona
authors.identifier: 0000-0001-7936-121X
authors.initials: S
authors.lastname: Visan
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Claudia
authors.initials: C
authors.lastname: Gherman
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Flaviu
authors.initials: F
authors.lastname: Drigla
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, University of Medicine and Pharmacy "I. Hatieganu", 23 Marinescu Street, 400337 Cluj-Napoca, Romania.
authors.forename: Laura
authors.initials: L
authors.lastname: Pop
authors.affiliation: Department of Senology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bolba-Morar
authors.affiliation: Department of Imagistics, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Carmen
authors.identifier: 0000-0002-5771-3584
authors.initials: C
authors.lastname: Lisencu
authors.affiliation: Department of Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Bogdan
authors.identifier: 0000-0001-8640-7546
authors.initials: B
authors.lastname: Fetica
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania.
authors.forename: Oana
authors.initials: O
authors.lastname: Tudoran
authors.affiliation: Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", 34-36 Republicii Street, 400015 Cluj-Napoca, Romania; Research Center for Functional Genomics, Biomedicine and Translational Medicine, University of Medicine and Pharmacy "I. Hatieganu", 23 Marinescu Street, 400337 Cluj-Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Berindan-Neagoe
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000172:deficiency / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases