title: MiR-15b mediates liver cancer cells proliferation through targeting BCL-2.
abstract: The incidence and mortality of liver cancer increased year by year. Our country presents high incidence of liver cancer. MicroRNAs have tissue sensitivity as tumor biomarkers that play a role by promoting tumor growth as oncogenes or inhibit malignant cell growth as tumor suppressor genes. Studies showed that miR-15b abnormal expression in the tumor and can be treated as one of the tumor molecular markers. However, miR-15b expression and role in the liver cancer cells have not been elucidated. This study intended to explore the mechanism of miR-15b effect on liver cancer occurrence and development. Liver cancer cell line HepG2 was transfected with miR-15b mimic or inhibitor. Real-time PCR was applied to detect miR-15b expression. MTT was used to test cell proliferation. Transwell assay was performed to determine cell invasive ability. Real-time PCR and Western blot were used to detect BCL2 expression. MiR-15b mimic transfection promoted miR-15b overexpression and inhibited HepG2 cell proliferation significantly (P < 0.05). MiR-15b overexpression downregulated BCL2 mRNA and protein expression obviously (P < 0.05). On the contrary, miR-15b inhibitor transfection markedly reduced miR-15b expression in liver cancer cells (P < 0.05), promoted tumor cell proliferation, and increased BCL2 mRNA and protein expression. MiR-15b expression changes did not affect cell invasion (P > 0.05). MiR-15b can inhibit HepG2 cell proliferation and down-regulate BCL2 mRNA and protein expression.
authors.affiliation: Department of General Surgery, The 3rd Xiangya Hospital of Central South University Changsha 410000, Hunan, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, The 3rd Xiangya Hospital of Central South University Changsha 410000, Hunan, China.
authors.forename: Feizhou
authors.initials: F
authors.lastname: Huang
authors.affiliation: Genetics Research Laboratory of CSU Changsha 410000, Hunan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Genetics Research Laboratory of CSU Changsha 410000, Hunan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Peng
authors.affiliation: Department of General Surgery, The 3rd Xiangya Hospital of Central South University Changsha 410000, Hunan, China.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Luo
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection
chemical_list: C000595707:BCL2 protein, human; D014408:Biomarkers, Tumor; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger
keywords: BCL2; Liver cancer; miR-15b; proliferation