title: Characterization of the Near Full-Length Genome of a Novel HIV-1 CRF01_AE/CRF07_BC Recombinant in an Injection Drug User from Southern Taiwan.
abstract: HIV-1 CRF07_BC became prevalent in Taiwan after the epidemic among injection drug users (IDUs). We describe a unique recombinant form (URF) consisting of CRF01_AE and CRF07_BC (named URF_0107-H8) genes detected from an IDU. The 8.8 kb near full-length genome of URF_0107-H8 had a CRF01_AE backbone with two CRF07_BC fragments in the reverse transcriptase and integrase region [RT-Int; HXB2 nucleotide (nt) positions 2942-4709] and within the envelop (nt 8467-8722) gene. Phylogenetic analyses revealed that its 1.8 kb RT-Int sequence clustered with those of CRF07_BC strains from Taiwan, while sequences of CRF01_AE portions were more similar to those of Central African origin than contemporaneous CRF01_AE isolates in Taiwan or prevalent in East or Southeast Asia. Recombination breakpoints and phylogenetic relationships of URF_0107-H8 were different from those of CRF01_AE/CRF07_BC URFs previously reported from China. This highlighted the importance of continual monitoring of genetic evolution of HIV strains and the emergence of new recombinants.
authors.affiliation: 1 Department of Medicine, College of Medicine, National Cheng Kung University , Tainan, Taiwan .|2 Department of Internal Medicine, National Cheng Kung University Hospital , Tainan, Taiwan .
authors.forename: Nan-Yao
authors.initials: NY
authors.lastname: Lee
authors.affiliation: 3 National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Tainan, Taiwan .
authors.forename: Jui-Ching
authors.initials: JC
authors.lastname: Wang
authors.affiliation: 3 National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Tainan, Taiwan .
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Lin
authors.affiliation: 1 Department of Medicine, College of Medicine, National Cheng Kung University , Tainan, Taiwan .|2 Department of Internal Medicine, National Cheng Kung University Hospital , Tainan, Taiwan .|3 National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Tainan, Taiwan .
authors.forename: Chi-Jung
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: 2 Department of Internal Medicine, National Cheng Kung University Hospital , Tainan, Taiwan .
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Li
authors.affiliation: 2 Department of Internal Medicine, National Cheng Kung University Hospital , Tainan, Taiwan .
authors.forename: Ming-Chi
authors.initials: MC
authors.lastname: Li
authors.affiliation: 4 Institute of Molecular Medicine, College of Medicine, National Cheng Kung University , Tainan, Taiwan .
authors.forename: Shainn-Wei
authors.initials: SW
authors.lastname: Wang
authors.affiliation: 3 National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes , Tainan, Taiwan .|5 Graduate Institute of Basic Medical Science, China Medical University , Taichung, Taiwan .|6 Department of Nursing, National Taipei University of Nursing and Health Sciences , Taipei, Taiwan .
authors.forename: Fan-Chen
authors.initials: FC
authors.lastname: Tseng
authors.affiliation: 1 Department of Medicine, College of Medicine, National Cheng Kung University , Tainan, Taiwan .|2 Department of Internal Medicine, National Cheng Kung University Hospital , Tainan, Taiwan .
authors.forename: Wen-Chien
authors.initials: WC
authors.lastname: Ko
mesh_terms: D000328:Adult; D055030:Drug Users; D016679:Genome, Viral; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D015819:Substance Abuse, Intravenous / Q000150:complications*; D013624:Taiwan