title: Abnormal galactosylation of immunoglobulin G in cerebrospinal fluid of multiple sclerosis patients.
abstract: Glycosylation alterations have been associated with the development of several human diseases and their animal models, including multiple sclerosis.
  
  
  
  We aimed to determine whether immunoglobulin G galactosylation might be changed in multiple sclerosis.
  
  
  
  Immunoglobulin G was isolated from serum and cerebrospinal fluid of patients with multiple sclerosis or viral meningitis and control patients without history of inflammatory or autoimmune disease. A lectin-based assay was used to investigate potential galactosylation modifications of immunoglobulin G.
  
  
  
  Galactosylation of immunoglobulin G isolated from cerebrospinal fluid of control patients was found to be age- and gender-dependent. In addition, immunoglobulin G galactosylation was significantly altered in cerebrospinal fluid but not in serum of multiple sclerosis patients. Furthermore, this modification was correlated with an active progression of multiple sclerosis. Finally, the loss of galactosyl moieties was not simply associated with inflammation as no such change was detected in viral meningitis patients characterized by brain inflammation.
authors.affiliation: Department of Neurology, Saarland University, Homburg/Saar, Germany Yann.Decker@uniklinikum-saarland.de.
authors.forename: Yann
authors.initials: Y
authors.lastname: Decker
authors.affiliation: Department of Neurology, Saarland University, Homburg/Saar, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Schomburg
authors.affiliation: Department of Neurology, Saarland University, Homburg/Saar, Germany.
authors.forename: Eszter
authors.initials: E
authors.lastname: Németh
authors.affiliation: Department of Neurology, Saarland University, Homburg/Saar, Germany.
authors.forename: Artem
authors.initials: A
authors.lastname: Vitkin
authors.affiliation: Department of Neurology, Saarland University, Homburg/Saar, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Fousse
authors.affiliation: Department of Neurology, Saarland University, Homburg/Saar, Germany.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurology, Saarland University, Homburg/Saar, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Fassbender
mesh_terms: D000328:Adult; D000367:Age Factors; D005260:Female; D005690:Galactose / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid / Q000378:metabolism*; D008297:Male; D008587:Meningitis, Viral / Q000097:blood / Q000134:cerebrospinal fluid*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid* / Q000503:physiopathology; D012737:Sex Factors
chemical_list: D007074:Immunoglobulin G; D005690:Galactose
keywords: Multiple sclerosis; immunology