title: Prospective Evaluation of a New Aspergillus IgG Enzyme Immunoassay Kit for Diagnosis of Chronic and Allergic Pulmonary Aspergillosis.
abstract: Anti-Aspergillus IgG antibodies are important biomarkers for the diagnosis of chronic pulmonary aspergillosis (CPA) and allergic bronchopulmonary aspergillosis (ABPA). We compared the performance of a new commercial enzyme immunoassay (EIA) (Bordier Affinity Products) with that of the Bio-Rad and Virion\Serion EIAs. This assay is novel in its association of two recombinant antigens with somatic and metabolic antigens of Aspergillus fumigatus In a prospective multicenter study, 436 serum samples from 147 patients diagnosed with CPA (136 samples/104 patients) or ABPA (94 samples/43 patients) and from 205 controls (206 samples) were tested. We obtained sensitivities of 97%, 91.7%, and 86.1%, and specificities of 90.3%, 91.3%, and 81.5% for the Bordier, Bio-Rad, and Virion\Serion tests, respectively. The Bordier kit was more sensitive than the Bio-Rad kit (P < 0.01), which was itself more sensitive than the Virion\Serion kit (P = 0.04). The Bordier and Bio-Rad kits had similar specificity (P = 0.8), both higher than that of the Virion\Serion kit (P = 0.02). The area under the receiver operating characteristic (ROC) curves confirmed the superiority of the Bordier kit over the Bio-Rad and the Virion\Serion kits (0.977, 0.951, and 0.897, respectively; P < 0.01 for each comparison). In a subset analysis of 279 serum samples tested with the Bordier and Bio-Rad kits and an in-house immunoprecipitin assay (IPD), the Bordier kit had the highest sensitivity (97.7%), but the IPD tended to be more specific (71.2 and 84.7%, respectively; P = 0.10). The use of recombinant, somatic, and metabolic antigens in a single EIA improved the balance of sensitivity and specificity, resulting in an assay highly suitable for use in the diagnosis of chronic and allergic aspergillosis.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: C
authors.initials: C
authors.lastname: Dumollard
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, CHU de Grenoble, Grenoble, France U823, Université Grenoble Alpes, Grenoble, France UMR 1137-IAME Team 5-DeSCID, Inserm/Paris Diderot, Université de la Sorbonne Paris Cité, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Bailly
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: S
authors.initials: S
authors.lastname: Perriot
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, CHU de Grenoble, Grenoble, France UMR 5163 CNRS, Université Grenoble Alpes, Grenoble, France.
authors.forename: M P
authors.initials: MP
authors.lastname: Brenier-Pinchart
authors.affiliation: Clinique Universitaire de Pneumologie, CHU Grenoble, Grenoble, France.
authors.forename: C
authors.initials: C
authors.lastname: Saint-Raymond
authors.affiliation: Clinique Universitaire de Pneumologie, CHU Grenoble, Grenoble, France.
authors.forename: B
authors.initials: B
authors.lastname: Camara
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHU de Rennes, Rennes, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Gangneux
authors.affiliation: Hospices Civils de Lyon, Laboratoire de Parasitologie-Mycologie, Hôpital de la Croix-Rousse, Lyon, France.
authors.forename: F
authors.initials: F
authors.lastname: Persat
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHU de Dijon, Dijon, France.
authors.forename: S
authors.initials: S
authors.lastname: Valot
authors.affiliation: Laboratoire de Parasitologie-Mycologie, CHU de Besançon, Besançon, France.
authors.forename: F
authors.initials: F
authors.lastname: Grenouillet
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, CHU de Grenoble, Grenoble, France UMR 5163 CNRS, Université Grenoble Alpes, Grenoble, France.
authors.forename: H
authors.initials: H
authors.lastname: Pelloux
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, CHU de Grenoble, Grenoble, France.
authors.forename: C
authors.initials: C
authors.lastname: Pinel
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, CHU de Grenoble, Grenoble, France Laboratoire TIMC-IMAG-TheREx, UMR 5525 CNRS, Université Grenoble Alpes, Grenoble, France mcornet@chu-grenoble.fr.
authors.forename: M
authors.identifier: 0000-0003-4155-3675
authors.initials: M
authors.lastname: Cornet
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000908:Antibodies, Fungal / Q000097:blood*; D001232:Aspergillus fumigatus / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D007223:Infant; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D055732:Pulmonary Aspergillosis / Q000175:diagnosis*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000908:Antibodies, Fungal; D007074:Immunoglobulin G