title: Digestion and absorption of an egg white ACE-inhibitory peptide in human intestinal Caco-2 cell monolayers.
abstract: The objective of this study was to investigate the digestion and absorption of egg white-derived angiotensin I-converting enzyme (ACE)-inhibitory peptide TNGIIR in human intestinal Caco-2 cell monolayers. Results showed that the digestion of TNGIIR to simulated gastrointestinal enzymes and brush border membrane peptidases were 5.87% ± 1.92% and 17.17% ± 0.64%, respectively (p < 0.05). The apparent permeability coefficients (P(app)) of TNGIIR from the apical to basolateral side in Caco-2 cell monolayers was determined to be (4.92 ± 0.40) × 10(-6) cm/s, indicating that TNGIIR can transport across Caco-2 cell monolayers in intact form. In addition, only cytochalasin D, a disruptor of tight junctions (TJs), changed TNGIIR transport rate significantly (p < 0.05), suggesting that the main transport route for TNGIIR across Caco-2 cell monolayers was paracellular pathway via TJs.
authors.affiliation: a Laboratory of Nutrition and Functional Food , Jilin University , Changchun , P.R. China ;
authors.forename: Long
authors.initials: L
authors.lastname: Ding
authors.affiliation: a Laboratory of Nutrition and Functional Food , Jilin University , Changchun , P.R. China ;
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.affiliation: b College of Food Science and Engineering, Bohai University , Jinzhou , P.R. China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Yu
authors.affiliation: a Laboratory of Nutrition and Functional Food , Jilin University , Changchun , P.R. China ;
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: a Laboratory of Nutrition and Functional Food , Jilin University , Changchun , P.R. China ;
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D001152:Arsenicals / Q000494:pharmacology; D001692:Biological Transport; D018938:Caco-2 Cells; D002352:Carrier Proteins; D015638:Cytochalasin D / Q000494:pharmacology; D004527:Egg Proteins / Q000378:metabolism; D004529:Egg White / Q000737:chemistry*; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D019810:Sodium Azide / Q000494:pharmacology; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D000806:Angiotensin-Converting Enzyme Inhibitors; D001152:Arsenicals; D002352:Carrier Proteins; D004527:Egg Proteins; D010446:Peptide Fragments; D010455:Peptides; C000589715:arginyl-valyl-prolyl-seryl-leucine; C029341:oxophenylarsine; D015638:Cytochalasin D; D019810:Sodium Azide; D000077191:Wortmannin
keywords: ACE-inhibitory peptide; Caco-2 cell monolayer; digestion; egg; transport