title: Blockade of cell adhesion molecules enhances cell engraftment in a murine model of liver cell transplantation.
abstract: OBJECTIVE
  OLT is the best alternative for patients with end-stage liver diseases. However, as the need for organs surpasses donor availability, alternatives to OLT are required. LCT could be a useful option versus OLT in several patients even though its low cell-engraftment hampers its efficiency. Endothelial cell barrier is the main obstacle for the implantation of cells into the parenchyma. Our study has focused on the modification of the endothelial barrier with monoclonal antibodies against adhesion molecules in order to increase cell engraftment in a mouse model of liver cell transplantation.
  
  
  METHODS
  Anti-mouse CD54 and anti-mouse CD61 antibodies were administered intrasplenically to healthy mice within 60 min prior to stem cell transplantation. Animals were sacrificed either short term at 2h or middle term seven days after transplantation. Immunohistochemical techniques to detect alkaline phosphatase activity were used to identify the transplanted cells within the liver parenchyma.
  
  
  RESULTS
  Anti-CD54 and anti-CD61 administration increases vascular patency and cell engraftment. This represents a 32% and 45% increase, respectively, of engrafted cells compared to the control (p<0.05).
  
  
  CONCLUSIONS
  Modification of the vascular wall with monoclonal antibodies against endothelial adhesion molecules before cell transplantation enhances cell engraftment into the mouse liver.
authors.affiliation: Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain. Electronic address: alfarogodayfj@gmail.com.
authors.forename: Javier
authors.initials: J
authors.lastname: Alfaro
authors.affiliation: Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Pérez
authors.affiliation: Department of General Surgery and Liver Transplantation, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Jiménez
authors.affiliation: Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Serrano
authors.affiliation: Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: José Ángel
authors.initials: JÁ
authors.lastname: Martínez-Flores
authors.affiliation: Department of General Surgery and Liver Transplantation, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Grau
authors.affiliation: Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Sánchez-Zapardiel
authors.affiliation: Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain; Section of Immunology, Universidad San Pablo-CEU. Madrid, Spain.
authors.forename: Estela
authors.initials: E
authors.lastname: Paz-Artal
authors.affiliation: Department of Immunology, Hospital Universitario 12 de Octubre, Madrid, Spain; Section of Immunology, Universidad San Pablo-CEU. Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Serrano
mesh_terms: D064591:Allografts; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D004195:Disease Models, Animal; D058625:End Stage Liver Disease / Q000276:immunology / Q000628:therapy*; D006085:Graft Survival / Q000187:drug effects* / Q000276:immunology; D061307:Human Umbilical Vein Endothelial Cells / Q000276:immunology; D006801:Humans; D039661:Integrin beta3 / Q000276:immunology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D008297:Male; D051379:Mice; D033581:Stem Cell Transplantation
chemical_list: D000906:Antibodies; D039661:Integrin beta3; D018799:Intercellular Adhesion Molecule-1
keywords: Adhesion molecules; Engraftment; Liver cell transplantation; Vascular patency