title: Characterization and antioxidant activities of extracellular and intracellular polysaccharides from Fomitopsis pinicola.
abstract: Fomitopsis pinicola (F. pinicola) is a kind of medicinal fungi, and few studies has been carried out on F. pinicola polysaccharides from liquid submerged cultivation. The characterization and antioxidant activities of extracellular polysaccharide (EPS) and intracellular polysaccharide (IPS) isolated from F. pinicola were investigated. The results showed that the molecular weight of EPS was 2.30×10(4)Da, and EPS was composed of mannose, rhamnose, xylose and galactose with the molar ratio of 0.1:1.0:0.3:0.5. The molecular weight of IPS was 4.07×10(5)Da, and the monosaccharide compositions included glucose, mannose, rhamnose, xylose and galactose with the molar ratio of 1.0:0.9:0.9:0.8:1.1. Antioxidant activities of both EPS and IPS including in vitro scavenging activities on 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals, cellular protective effects on yeast cells from ultraviolet (UV) radiation and H2O2 oxidative damage were tested. Both EPS and IPS showed antioxidant activities in a dose dependent manner, and IPS had higher antioxidant activity than EPS. So EPS and IPS could be potential novel antioxidants for functional food.
authors.affiliation: The Quartermaster Equipment Institute of General Logistics Department of People's Liberation Army, Beijing 100010, China; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China. Electronic address: hlm2005@163.com.
authors.forename: Limin
authors.initials: L
authors.lastname: Hao
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China.
authors.forename: Zhicun
authors.initials: Z
authors.lastname: Sheng
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China. Electronic address: ljk002004@163.com.
authors.forename: Jike
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China.
authors.forename: Ruyu
authors.initials: R
authors.lastname: Tao
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China.
authors.forename: Shiru
authors.initials: S
authors.lastname: Jia
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D055453:Coriolaceae / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides
keywords: Antioxidant activities; Characteristics; Extracellular polysaccharide; Fomitopsis pinicola; Intracellular polysaccharide