title: Novel electrically conducting 2-hydroxyethylcellulose/polyaniline nanocomposite cryogels: Synthesis and application in tissue engineering.
abstract: Novel electrically conducting 2-hydroxyethylcellulose/polyaniline (HEC/PANI) nanocomposite cryogels were fabricated via the combination of cryogenic treatment and photochemical crosslinking. PANI nanofillers (one-dimentional tubes and three-dimentional particles) were synthesized via oxidative polymerization of aniline in aqueous media and, then, embedded in the HEC matrix. The effect of PANI content and morphology on the gel fraction yield and electrical conductivity of material was studied. Nanocomposite cryogels of high gel fraction yield (65-95%) and rather high electrical conductivity (0.02-0.1S/m) were obtained by using a relatively small amount (0.5-3wt.% to HEC) of pre-formed PANI nanofillers. The behavior of L929 cells adhered on HEC/PANI cryogels in the presence of electric field were also investigated. Cytotoxicity test showed very good survival and proliferation of cells on cryogels, while the electrical stimulation triggered changes in cell morphology as well as a specific alignment of cells in parallel to the electrical field.
authors.affiliation: Institute of Polymers, Bulgarian Academy of Sciences, Akad. G. Bonchev st., bl. 103A, 1113 Sofia, Bulgaria. Electronic address: ppetrov@polymer.bas.bg.
authors.forename: Petar
authors.initials: P
authors.lastname: Petrov
authors.affiliation: Institute of Polymers, Bulgarian Academy of Sciences, Akad. G. Bonchev st., bl. 103A, 1113 Sofia, Bulgaria.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Mokreva
authors.affiliation: Institute of Polymers, Bulgarian Academy of Sciences, Akad. G. Bonchev st., bl. 103A, 1113 Sofia, Bulgaria.
authors.forename: Ivan
authors.initials: I
authors.lastname: Kostov
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Akad. G. Bonchev st., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Veselina
authors.initials: V
authors.lastname: Uzunova
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Akad. G. Bonchev st., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Rumiana
authors.initials: R
authors.lastname: Tzoneva
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic; D059985:Cryogels / Q000737:chemistry*; D004553:Electric Conductivity; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D000814:Aniline Compounds; D001672:Biocompatible Materials; D059985:Cryogels; C416807:polyaniline; D002482:Cellulose; C002283:hydroxyethylcellulose
keywords: 2-Hydroxyethylcellulose; Cell rearrangement; Cell viability; Conducting cryogels; Polyaniline; Tissue engineering