title: Sevoflurane inhibits the antioxidant capacity of erythrocytes.
abstract: The aim of the present study was to observe the effects of sevoflurane on the antioxidant capacity, endothelial nitric oxide synthase (eNOS) content and lifespan of erythrocytes. A 2% erythrocyte suspension was prepared from whole blood collected from healthy volunteers and then treated with sevoflurane at different concentrations (group A, 0%; group S1, 1%; group S3, 3%; and group S5, 5%), in the presence or absence of 200 µmol/l hydrogen peroxide (H2O2, or H in group names). In order to evaluate the effects of sevoflurane on the antioxidant capacity and NO metabolism of erythrocytes, the hemolysis rate, catalase (CAT) content and eNOS content were determined, while the labeled phosphatidylserine rate and forward scatter of erythrocytes were detected using flow cytometry. Group S3 showed the highest hemolysis rate in the absence H2O2, while treatment with H2O2increased the hemolysis rate of groups S1 and S3 (P=0.027). The CAT content in groups treated with sevoflurane was significantly lower compared with that in the control (group A, air group). The CAT content in groups S1+H, S3+H and S5+H remained significantly lower compared with group A+H (P<0.05). The eNOS content of group A was similar to that of group S3, while the content in group S1 was similar to that in group S5. In addition, the eNOS content of groups A and S3 increased, while that of groups S1 and S5 was reduced upon H2O2treatment (P<0.05). The results indicated that sevoflurane reduced the antioxidative activity of erythrocytes, decreasing the resistant ability to H2O2damage and increasing the hemolysis rate. The underlying mechanism may be associated with the inhibitory effect on the CAT activity of erythrocytes. Sevoflurane also inhibited the generation of nitric oxide in erythrocytes and reduced the tolerance of erythrocytes against oxidative stress damage due to H2O2.
authors.affiliation: Department of Anesthesia, Air Force General Hospital, PLA, Beijing 100142, P.R. China.
authors.forename: B O
authors.initials: BO
authors.lastname: Ye
authors.affiliation: Department of Anesthesia, Air Force General Hospital, PLA, Beijing 100142, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Outpatient Department, Shanghai Campus, PLA Nanjing Institute of Politics, Shanghai 200433, P.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Department of Pain, Air Force General Hospital, PLA, Beijing 100142, P.R. China.
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Zhang
authors.affiliation: Department of Anesthesia, Air Force General Hospital, PLA, Beijing 100142, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Anesthesia, Air Force General Hospital, PLA, Beijing 100142, P.R. China.
authors.forename: Guo
authors.initials: G
authors.lastname: Wei
authors.affiliation: Department of Anesthesia, Air Force General Hospital, PLA, Beijing 100142, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Department of Anesthesia, Air Force General Hospital, PLA, Beijing 100142, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
keywords: catalase; decay; erythrocyte; nitric oxide; sevoflurane