title: Association between premature ovarian failure, polymorphisms in MTHFR and MTRR genes and serum homocysteine concentration.
abstract: This study investigated the association between premature ovarian failure (POF), MTHFR C677T/A1298C and MTRR A66G genotypes and serum homocysteine (Hcy) concentration. A prospective study was conducted in Chinese women, which included POF patients (n = 180) and controls (n = 195). Peripheral blood samples were used to determine MTHFR C677T/A1298C and MTRR A66G genotypes, and serum Hcy and sex hormone concentrations. Results showed that serum Hcy concentrations of POF patients were significantly higher than those of controls (P < 0.0001). In POF patients, serum Hcy concentrations were significantly correlated with oestradiol and FSH concentrations (r = -0.174, P = 0.037 and r = +0.238, P = 0.006, respectively). There were no significant differences in the distributions of MTHFR C677T/A1298C or MTRR A66G genotypes between the two groups. However, these genetic variants influenced serum Hcy concentrations in POF patients, especially for MTRR 66 AA/AG/GG genotypes, which were significantly correlated with the patients' Hcy concentrations (τ = 0.166, P = 0.033). These results suggest that serum Hcy concentrations in Chinese POF patients are increased and correlated with serum oestradiol/FSH concentrations. In conclusion, MTHFR C667T/A1298C and MTRR A66G genotypes are not associated with POF development, but they affect the patients' serum Hcy concentrations.
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou 310006, China; Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Hou
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou 310006, China; International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Songchang
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou 310006, China; Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Institute of Public Administration, Zhejiang Normal University, Hangzhou 310012, China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Jiang
authors.affiliation: International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Junyu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou 310006, China; Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Bai
authors.affiliation: International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou 310006, China; International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China. Electronic address: huanghefg@sjtu.edu.cn.
authors.forename: Hefeng
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Reproductive Genetics, Ministry of Education (Zhejiang University), Hangzhou 310006, China; International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China. Electronic address: chenming_xu2006@163.com.
authors.forename: Chenming
authors.initials: C
authors.lastname: Xu
mesh_terms: D000328:Adult; D004958:Estradiol / Q000097:blood; D005260:Female; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D005640:Follicle Stimulating Hormone / Q000097:blood; D056726:Genetic Association Studies; D005838:Genotype; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D011110:Polymorphism, Genetic; D016649:Primary Ovarian Insufficiency / Q000235:genetics*; D011446:Prospective Studies
chemical_list: D006710:Homocysteine; D004958:Estradiol; D005640:Follicle Stimulating Hormone; C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: MTHFR; MTRR; gene polymorphism; homocysteine; premature ovarian failure