title: Imbalance of Th17/Tregs in rats with smoke inhalation-induced acute lung injury.
abstract: T helper (Th) 17 cells and CD4(+) CD25(+) regulatory T (Treg) cells are supposed to be critically involved in regulating autoimmune and inflammatory diseases. The aim of this study was to investigate the Th17/Treg pattern in rats with gunpowder smog-induced acute lung injury. Wistar rats were equally randomized to three groups: normal control group, ALI 6 h group (smoke inhalation for 6 h) and ALI 24 h group (smoke inhalation for 24 h). We observed changes in cell counting in bronchoalveolar lavage fluid (BALF), alveolar-capillary membrane permeability and lung tissue pathology. Moreover, rats in ALI 6 h and ALI 24 h group showed increased expression of Th17 cell and related cytokines (IL-17 A, IL-6, TGF-β and IL-23). Meanwhile, Treg prevalence and related cytokines (IL-10, IL-2 and IL-35) were decreased. Consequently, the ratio of Th17/Treg was higher after smoke inhalation. Additionally, Th1 cell decreased while Th2 cell increased at 6 h and 24 h after smoke inhalation. In conclusion, Th17/Treg imbalance exists in rats with smoke inhalation-induced acute lung injury, suggesting its potential role in the pathogenesis of this disease.
authors.affiliation: Department of Clinical Laboratory Medicine, Chinese People's Liberation Army General Hospital &Postgraduate Medical School, Beijing 100853, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory Medicine, Chinese People's Liberation Army General Hospital &Postgraduate Medical School, Beijing 100853, People's Republic of China.
authors.forename: Mian-yang
authors.initials: MY
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Medicine, Chinese People's Liberation Army General Hospital &Postgraduate Medical School, Beijing 100853, People's Republic of China.
authors.forename: Ya-ting
authors.initials: YT
authors.lastname: Lan
authors.affiliation: Department of Clinical Laboratory Medicine, Chinese People's Liberation Army General Hospital &Postgraduate Medical School, Beijing 100853, People's Republic of China.
authors.forename: Cheng-bin
authors.initials: CB
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002199:Capillary Permeability; D016130:Immunophenotyping; D007958:Leukocyte Count; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D012906:Smoke / Q000009:adverse effects / Q000032:analysis; D015208:Smoke Inhalation Injury / Q000150:complications* / Q000276:immunology* / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D012906:Smoke; D009195:Peroxidase