title: Bacteriophage can lyse antibiotic-resistant Pseudomonas aeruginosa isolated from canine diseases.
abstract: Pseudomonas aeruginosa is a pathogen frequently identified as the cause of diverse infections or chronic disease. This microbe has natural resistance to several kinds of antibiotics, because of the species' outer membrane, efflux pumps and growth as a biofilm. This bacterium can acquire increased resistance with specific point mutations. Bacteriophage (phage), however, can lyse these bacteria. Therefore, in the present study, we assessed the host range of phages isolates and their ability to lyse antibiotic-resistant P. aeruginosa. Present phages could lyse many strains of P. aeruginosa (28/39), including strains with high resistance to fluoroquinolones (4/6). In conclusion, application of phages for antibiotic-resistant bacteria is greatly effective. To avoid pervasive antibiotic-resistant bacteria, further development of phage usage for disease treatment is required.
authors.affiliation: Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Furusawa
authors.forename: Hidetomo
authors.initials: H
authors.lastname: Iwano
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Higuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokota
authors.forename: Masaru
authors.initials: M
authors.lastname: Usui
authors.forename: Tomohito
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamura
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000502:physiology* / Q000648:ultrastructure; D004283:Dog Diseases / Q000382:microbiology* / Q000821:virology; D004285:Dogs; D024881:Drug Resistance, Bacterial; D008854:Microscopy, Electron; D011552:Pseudomonas Infections / Q000382:microbiology / Q000662:veterinary* / Q000821:virology; D011550:Pseudomonas aeruginosa / Q000821:virology*