title: Association between p21 Ser31Arg polymorphism and the development of cervical lesion in women infected with high risk HPV.
abstract: Infection by high-risk human papillomavirus (HR-HPV) and single nucleotide polymorphism (SNP) in genes involved in cell cycle control, as p21 and p27, are important factors in the development of different types of human cancers. This study aims at investigating whether both the p21 Ser31Arg and p27 V109G polymorphisms are associated with susceptibility to the development of cervical lesions in women HR-HPV positive. We analyzed 132 women HPV positive and with cervical lesions or CC and 154 healthy control (HPV negative and without cervical lesions). p21 Ser31Arg and p27 V109G polymorphisms were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and sequencing. The p21 31Arg allele was associated with susceptibility for the development of cervical lesions (P* = 0.0009), while p27 V109G polymorphism showed no significant differences for this association (P* = 0.89). However, the combined effect of the polymorphisms showed that the presence of the CC genotype (SNP p21 Ser31Arg) conferred protection for the development of cervical lesions (OR = 0.39). p21 Ser31Arg and p27 V109G polymorphisms were not associated with the grade of cervical lesions (CINI, CINII, and CINIII) or CC (P* > 0.05). The HR-HPV more frequent in this study were of 16 (57.6 %) and 18 (37.1 %) types; however, no association was observed when both polymorphisms and risk factors analyzed were compared (P* > 0.05). Our findings suggest a possible association between p21 Ser31tabArg polymorphism and susceptibility to the development of cervical lesions in women from Pernambuco. Brazil.
authors.affiliation: Post-Graduate Program in Cellular and Molecular Biology Applied, University of Pernambuco (UPE), Recife, PE, Brazil.
authors.forename: Géssica
authors.initials: G
authors.lastname: Lima
authors.affiliation: Post-Graduate Program in Tropical Animal Science, Rural Federal University of Pernambuco (UFRPE), Recife, PE, Brazil.
authors.forename: Erinaldo
authors.initials: E
authors.lastname: Santos
authors.affiliation: Post-Graduate Program in Genetic, Federal University of Pernambuco (UFPE), Recife, PE, Brazil.|Federal Institute of Pernambuco - Campus Garanhuns (IFPE/Garanhuns), Garanhuns, PE, Brazil.
authors.forename: Hildson
authors.initials: H
authors.lastname: Angelo
authors.affiliation: Post-Graduate Program in Biological Science, Federal University of Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Micheline
authors.initials: M
authors.lastname: Oliveira
authors.affiliation: Departament of Lower Genital Tract Pathology, Women's Healthcare Center, Instituto de Medicina Integral Prof. Fernando Figueira (IMIP), Recife, PE, Brazil.
authors.forename: Sandra
authors.initials: S
authors.lastname: Heráclio
authors.affiliation: Department of Biology, Rural Federal University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, PE, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Leite
authors.affiliation: Departament of Physics, Federal University of Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Celso
authors.initials: C
authors.lastname: de Melo
authors.affiliation: Post-Graduate Program in Genetic, Federal University of Pernambuco (UFPE), Recife, PE, Brazil.|Departament of Genetics, Federal University of Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Crovella
authors.affiliation: Post-Graduate Program in Tropical Animal Science, Rural Federal University of Pernambuco (UFRPE), Recife, PE, Brazil.|Department of Biology, Rural Federal University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, PE, Brazil.
authors.forename: Maria
authors.initials: M
authors.lastname: Maia
authors.affiliation: Post-Graduate Program in Cellular and Molecular Biology Applied, University of Pernambuco (UPE), Recife, PE, Brazil. prsouza30@gmail.com.|Post-Graduate Program in Tropical Animal Science, Rural Federal University of Pernambuco (UFRPE), Recife, PE, Brazil. prsouza30@gmail.com.|Department of Biology, Rural Federal University of Pernambuco (UFRPE), Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, PE, Brazil. prsouza30@gmail.com.
authors.forename: Paulo
authors.initials: P
authors.lastname: Souza
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000821:virology
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D050760:Cyclin-Dependent Kinase Inhibitor p27
keywords: Cell cycle; Cervical cancer; HPV; SNPs