title: Differential expression network analysis for diabetes mellitus type 2 based on expressed level of islet cells.
abstract: OBJECTIVE
  Diabetes mellitus type 2 (T2DM) is a metabolic disease that has become a pressing issue, with potential adverse impact on mental health. We aimed to explore the potential molecular mechanism of T2DM.
  
  
  METHODS
  GSE38642 microarray data downloaded from gene expression omnibus was used to identify the differentially expressed genes (DEGs). Profiling of complex functionality (ProfCom) was used to analyze the complex function and mine T2DM signature genes. Finally, the differential expression network (DEN) was constructed.
  
  
  RESULTS
  We identified 147 DEGs including 59 up- and 88 down-regulated genes. With increasing of degree, the specificity of functional description of DEGs was higher. GO term of "integral to membrane and immune response (not receptor activity) not regulation of immune response" in degree 4 was enriched by 6 DEGs, while the GO term of "immune response" in degree 1 was enriched by 12 DEGs. Two complex functions of integral to membrane an immune response and response to glucose stimulus were enriched by 11 T2DM signature genes including ARG2, GLP1R, PFKFB2, PTPRN, ACSL5, CCR7, IL2RA, IL7R, IL1R2, IL1RL1 and CHST4. Finally, DEN including 11 signature genes and 491 edges was obtained.
  
  
  CONCLUSIONS
  The identified DEGs especially 11 signature genes such as PTPRN, GLP1R, CCR7 and IL2RA may play important roles in the pathogenesis of T2DM.
authors.affiliation: Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013 Shandong Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Neurology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013 Shandong Province, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013 Shandong Province, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Chi
authors.affiliation: Department of Cardiology, Jinan Central Hospital Affiliated to Shandong University, Jinan, No. 105, Jiefang Road, Jinan City, 250013 Shandong Province, China. Electronic address: leiwang1091@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism*
keywords: Diabetes mellitus type 2; Diabète de type 2; Differential expression network; Profilage des fonctionnalités complexes; Profiling of complex functionality; Réseau d’expression différentielle