title: Association Between Gasdermin A and Gasdermin B Polymorphisms and Susceptibility to Adult and Childhood Asthma Among Jordanians.
abstract: BACKGROUND
  Gasdermin A (GSDMA) and gasdermin B (GSDMB) have been associated with childhood, and to a lesser extent with adult, asthma in many populations.
  
  
  OBJECTIVE
  In this study, we investigated the association between GSDMA and GSDMB variants and the incidence of adult and childhood asthma among Jordanians.
  
  
  METHODS
  Subjects were divided into two groups: adults and children. Within the adult group there were 129 asthma patients and 111 healthy controls. In the pediatric group there were 98 asthma patients and 112 healthy children. Gasdermin A (GSDMA) (rs7212938, T/G) and Gasdermin B (rs7216389, T/C) polymorphisms were genotyped using the PCR-RFLP method. Three analysis models were applied to the genotype data: co-dominant, dominant and recessive.
  
  
  RESULTS
  An association between the GSDMB T/C single nucleotide polymorphism (SNP) genotype and the incidence of childhood asthma was found (< 0.05). GSDMB T/C SNP in children also showed a very high tendency toward significance with p = 0.0532 in the single locus analysis. In adults, no significant differences in the allelic frequencies of any of the SNPs analyzed were found between the case and control populations. At the haplotype level, GC haplotype was found to be associated with the risk of asthma in children while none of the tested haplotypes were found to be associated with asthma risk in adults.
  
  
  CONCLUSIONS
  The findings of this study confirm the previously reported association between the GSDMB gene and the risk of childhood asthma.
authors.affiliation: 1 Department of Pharmacology, Faculty of Medicine, The University of Jordan , Amman, Jordan .
authors.forename: Malek
authors.initials: M
authors.lastname: Zihlif
authors.affiliation: 2 Department of Internal Medicine, University of Jordan Hospital, The University of Jordan , Amman, Jordan .
authors.forename: Nathir M
authors.initials: NM
authors.lastname: Obeidat
authors.affiliation: 3 Department of Pediatric, Jordan Hospital , Amman, Jordan .
authors.forename: Nadwa
authors.initials: N
authors.lastname: Zihlif
authors.affiliation: 4 Department of Special Surgery, University of Jordan Hospital, The University of Jordan , Amman, Jordan .
authors.forename: Tareq
authors.initials: T
authors.lastname: Mahafza
authors.affiliation: 5 Department of Biotechnology and Genetic Engineering, Faculty of Science, Philadelphia University , Amman, Jordan .
authors.forename: Tawfiq
authors.initials: T
authors.lastname: Froukh
authors.affiliation: 6 Faculty of Medicine, The University of Jordan , Amman, Jordan .
authors.forename: Marcel T
authors.initials: MT
authors.lastname: Ghanim
authors.affiliation: 6 Faculty of Medicine, The University of Jordan , Amman, Jordan .
authors.forename: Hamza
authors.initials: H
authors.lastname: Beano
authors.affiliation: 7 Department of Physiology and Biochemistry, Faculty of Medicine, The University of Jordan , Amman, Jordan .
authors.forename: Fatima M
authors.initials: FM
authors.lastname: Al-Akhras
authors.affiliation: 7 Department of Physiology and Biochemistry, Faculty of Medicine, The University of Jordan , Amman, Jordan .
authors.forename: Randa
authors.initials: R
authors.lastname: Naffa
mesh_terms: D000328:Adult; D000367:Age Factors; D001249:Asthma / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D000094524:Gasdermins
chemical_list: D009363:Neoplasm Proteins; C485887:GSDMA protein, human; D000094524:Gasdermins; C527978:GSDMB protein, human