title: Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine.
abstract: Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida.
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil. Electronic address: thales.furian@ufrgs.br.
authors.forename: Thales Quedi
authors.initials: TQ
authors.lastname: Furian
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil.
authors.forename: Karen Apellanis
authors.initials: KA
authors.lastname: Borges
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Laviniki
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil.
authors.forename: Silvio Luis da Silveira
authors.initials: SL
authors.lastname: Rocha
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil.
authors.forename: Camila Neves
authors.initials: CN
authors.lastname: de Almeida
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil.
authors.forename: Vladimir Pinheiro
authors.initials: VP
authors.lastname: do Nascimento
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil.
authors.forename: Carlos Tadeu Pippi
authors.initials: CT
authors.lastname: Salle
authors.affiliation: Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS CEP: 91540-000, Brazil.
authors.forename: Hamilton Luiz de Souza
authors.initials: HL
authors.lastname: Moraes
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005838:Genotype; D008826:Microbial Sensitivity Tests; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D011200:Poultry; D011201:Poultry Diseases / Q000382:microbiology*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Antimicrobial susceptibility; Multiplex-PCR; Pasteurellosis; Virulence factors