title: Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers. I. Design of Experiments-Assisted Development.
abstract: The aim of the present work was the development of sponge-like dressings based on chitosan glutamate (high molecular weight) and sericin for the treatment of chronic skin ulcers. Dressings were prepared by freeze-drying and glycine was added as cryoprotectant agent. Dressing development was assisted by design of experiments, using a simplex centroid design. Mechanical resistance, hydration propensity, viscous, and viscoelasticity properties of dressings were considered as response variables. The superimposition of the contour plots, calculated by the best fit model for each response variable, permitted to individuate a region of the factor space where the dressing of optimized quantitative composition was chosen. Such a dressing was able to absorb high amount of phosphate-buffered saline forming a gel characterized by rheological properties enabling both a lubricant and a protective effect. The optimized formulation was characterized by optimal mechanical properties and by cell proliferation and antioxidant activity on human fibroblast cell line.
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Mori
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy. Electronic address: silvia.rossi@unipv.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bonferoni
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Theodora
authors.initials: T
authors.lastname: Chlapanidas
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Torre
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
mesh_terms: D000818:Animals; D001458:Bandages; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects; D005612:Freeze Drying / Q000379:methods; D018698:Glutamic Acid / Q000737:chemistry / Q000494:pharmacology; D005998:Glycine / Q000737:chemistry; D006801:Humans; D011161:Porifera / Q000737:chemistry*; D012212:Rheology / Q000379:methods; D047030:Sericins / Q000737:chemistry* / Q000494:pharmacology*; D012883:Skin Ulcer / Q000188:drug therapy*; D014783:Viscosity; D014945:Wound Healing / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D047030:Sericins; D018698:Glutamic Acid; D048271:Chitosan; D005998:Glycine
keywords: antioxidants; cell culture; chitosan; polymeric biomaterials; wound healing