title: miR-92b regulates glioma cells proliferation, migration, invasion, and apoptosis via PTEN/Akt signaling pathway.
abstract: Glioblastoma (GBM) is a highly invasive malignant primary brain tumor with neoplastic growth. Despite the progresses made in surgery, chemotherapy, and radiation in recent decade, the prognosis of patients with gliomas remains poor and the average survival time of patients suffering from glioblastoma is still short. As a potential therapy strategy, microRNAs have been considered as new targets for possible cancer treatment. In this study, we found that the miR-92b inhibitors (miR-92b-I) could inhibit the proliferation, migration, invasion, and promote the apoptosis of glioma cells. As a predicted target of miR-92b, phosphatase and tensin homolog (PTEN), also elevated at both mRNA and protein levels. Moreover, the Akt phosphorylation was consistently inhibited. The rescue experiment with miR-92b and PTEN double knockdown resulted in partial reversion of miR-92b-I-induced phenotypes. Taken together, our findings indicated that miR-92b-I could restrain the proliferation, invasion, migration, and stimulate apoptosis of glioma cells by targeting PTEN/Akt signaling pathway. Further investigations will focus on antitumor effect of miR‑92b-I in glioma treatment.
authors.affiliation: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, #2 Sipailou Road, Nanjing, 210096, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Song
authors.affiliation: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, #2 Sipailou Road, Nanjing, 210096, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, #2 Sipailou Road, Nanjing, 210096, China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.affiliation: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, #2 Sipailou Road, Nanjing, 210096, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wan
authors.affiliation: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, #2 Sipailou Road, Nanjing, 210096, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical School of Southeast University, #87 Dingjiaqiao Road, Nanjing, 210009, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical School of Southeast University, #87 Dingjiaqiao Road, Nanjing, 210009, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Kong
authors.affiliation: State Education Ministry's Key Laboratory of Developmental Genes and Human Diseases, Southeast University, #2 Sipailou Road, Nanjing, 210096, China. yld@seu.edu.cn.|Department of Biochemistry and Molecular Biology, Medical School of Southeast University, #87 Dingjiaqiao Road, Nanjing, 210009, China. yld@seu.edu.cn.
authors.forename: Liudi
authors.initials: L
authors.lastname: Yuan
mesh_terms: D020413:3' Untranslated Regions / Q000187:drug effects; D000070416:Antagomirs / Q000494:pharmacology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019295:Computational Biology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D017930:Genes, Reporter / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009154:Mutation; D051059:PTEN Phosphohydrolase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D012334:RNA, Neoplasm / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D020413:3' Untranslated Regions; D000070416:Antagomirs; D000964:Antimetabolites, Antineoplastic; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: Akt; Carcinogenesis; Glioblastomas; PTEN; miR-92b