title: Nailfold videocapillaroscopy and serum VEGF levels in scleroderma are associated with internal organ involvement.
abstract: OBJECTIVE
  Nailfold videocapillaroscopy (NVC) identifies the microvascular hallmarks of systemic sclerosis (SSc) and vascular endothelial growth factor (VEGF) and may play a pivotal role in the associated vasculopathy. The aim of the present study was to compare NVC alterations with clinical subsets, internal organ involvement, and serum VEGF levels in a cohort of selected SSc cases.
  
  
  METHODS
  We studied 44 patients with SSc who were evaluated within 3 months from enrollment by NVC, skin score, severity index, pulmonary function tests, carbon monoxide diffusing capacity (DLCO), echocardiography, pulmonary high-resolution computed tomography (HRCT), gastroesophageal (GE) endoscopy or manometry or X-ray, and serum autoantibodies. Serum VEGF-A levels were determined by ELISA in 72 SSc patients and 31 healthy controls.
  
  
  RESULTS
  Giant capillaries were inversely correlated with age (p = 0.034, r = -0.34) and to the extent of reticular pattern at HRCT (p = 0.04, r = -0.5). Avascular areas were directly correlated with capillaroscopy skin ulcer risk index (CSURI) (p = 0.006, r = +0.4) and severity index (p = 0.004, r = +0.5). The mean capillary density was directly correlated to the ulcer number (p = 0.02, r = +0.4) and to DLCO/alveolar volume (p = 0.02, r = +0.4) and inversely correlated with severity index (p = 0.01, r = -0.4) and skin score (p = 0.02, r = -0.4). Serum VEGF levels were higher in the SSc population vs controls (p = 0.03) and inversely correlated with DLCO (p = 0.01, r =-0.4) and directly with ground-glass and reticular pattern at HRCT (p = 0.04, r = +0.4 for both).
  
  
  CONCLUSIONS
  Our data suggest the importance of NVC not only for the diagnosis, but also for the global evaluation of SSc patients. Of note, serum VEGF levels may act as a biomarker of interstitial lung involvement.
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: De Santis
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.|BIOMETRA Department, University of Milan, Milan, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Ceribelli
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavaciocchi
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Crotti
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Massarotti
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.|Division of Rheumatology, Niguarda Hospital, Milan, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Belloli
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.
authors.forename: Bianca
authors.initials: B
authors.lastname: Marasini
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.
authors.forename: Natasa
authors.initials: N
authors.lastname: Isailovic
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Generali
authors.affiliation: Rheumatology and Clinical Immunology, Humanitas Research Hospital, Rozzano, Milan, Italy. carlo.selmi@unimi.it.|BIOMETRA Department, University of Milan, Milan, Italy. carlo.selmi@unimi.it.
authors.forename: Carlo
authors.initials: C
authors.lastname: Selmi
keywords: Autoimmunity; Biomarkers; Endothelium; Personalized medicine; Systemic sclerosis