title: Cryopreservation of ovaries from neonatal marmoset monkeys.
abstract: The ovary of neonatal nonhuman primates contains the highest number of immature oocytes, but its cryopreservation has not yet been sufficiently investigated in all life stages. In the current study, we investigated cryodamage after vitrification/warming of neonatal ovaries from a nonhuman primate, the common marmoset (Callithrix jacchus). A Cryotop was used for cryopreservation of whole ovaries. The morphology of the vitrified/warmed ovaries was found to be equivalent to that of fresh ovaries. No significant difference in the number of oocytes retaining normal morphology per unit area in histological sections was found between the two groups. In an analysis of dispersed cells from the ovaries, however, the cell viability of the vitrified/warmed group tended to be decreased. The results of a comet assay showed no significant differences in DNA damage. These results show that cryopreservation of neonatal marmoset ovaries using vitrification may be useful as a storage system for whole ovaries.
authors.affiliation: Department of Neurophysiology, National Institute of Neuroscience (NIN), National Center for Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
authors.forename: Hideyuki H
authors.initials: HH
authors.lastname: Motohashi
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ishibashi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002144:Callithrix; D002452:Cell Count; D020552:Comet Assay; D015925:Cryopreservation / Q000379:methods*; D004249:DNA Damage; D005260:Female; D009865:Oocytes; D010053:Ovary / Q000166:cytology* / Q000473:pathology; D014021:Tissue Preservation / Q000379:methods*; D058989:Vitrification