title: Therapeutic effects of OP-1 on metal wear particle induced osteoblasts injury in vitro.
abstract: Aseptic lossening is a main reason for the revision of total joint arthroplasty. Metal-wear particles induced deregulation of bone resorption or formation has been considered as the major process of aseptic lossening. Osteogenic protein-1 (OP-1) can be used to improve bone formation. However, such effect is not clearly understood after the metal-wear particles injury. Here, we investigated the molecular mechanisms by which OP-1 regulates the activity of bone formation and anti-inflammatory after injury. Results showed that OP-1 increased cell viability and bone formation ability of impaired osteoblast cells at 72 hours after being injured by cobalt particles. Pathway analyses revealed that both mRNA and protein levels of Smad1 and Smad5 were significantly increased upon the treatment of OP-1 in the cell injury model. Similarly, runt-related transcription factor 2 (Runx2) was also significantly upregulated in the OP-1 treated cells. Moreover, treatment with OP-1 inhibited the secretion of interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), and IL-18 in cobalt impaired cells. Collectively, these results suggest that OP-1 could inhibit cobalt particles induced cell injury by activating Smad1, Smad5, and Runx2, and such procedure is accompanied by anti-inflammatory reaction.
authors.affiliation: Department of Orthopedics, Jinling Hospital Nanjing 210002, Jiangsu, China.
authors.forename: Guojing
authors.initials: G
authors.lastname: Sun
authors.affiliation: Department of Orthopedics, The 81st Hospital of PLA Nanjing 210002, Jiangsu, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The 81st Hospital of PLA Nanjing 210002, Jiangsu, China.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Yang
authors.affiliation: DLPartner Immunotherapeutic Research Lab MD 20742, United States.
authors.forename: Thomas Mn
authors.initials: TM
authors.lastname: Parker
authors.affiliation: DLPartner Immunotherapeutic Research Lab MD 20742, United States.
authors.forename: Gary Mp
authors.initials: GM
authors.lastname: Goodman
authors.affiliation: DLPartner Immunotherapeutic Research Lab MD 20742, United States.
authors.forename: Jack M
authors.initials: JM
authors.lastname: Hasama
authors.affiliation: Department of Orthopedics, Jinling Hospital Nanjing 210002, Jiangsu, China.
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhao
keywords: Bone morphogenetic pritein-7; cobalt wear particles; osteoblast formation