title: A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium.
abstract: The process of mammary epithelial morphogenesis is influenced by hormones. The study of hormone action on the breast epithelium using 2D cultures is limited to cell proliferation and gene expression endpoints. However, in the organism, mammary morphogenesis occurs in a 3D environment. 3D culture systems help bridge the gap between monolayer cell culture (2D) and the complexity of the organism. Herein, we describe a 3D culture model of the human breast epithelium that is suitable to study hormone action. It uses the commercially available hormone-responsive human breast epithelial cell line, T47D, and rat tail collagen type 1 as a matrix. This 3D culture model responds to the main mammotropic hormones: estradiol, progestins and prolactin. The influence of these hormones on epithelial morphogenesis can be observed after 1- or 2-week treatment according to the endpoint. The 3D cultures can be harvested for analysis of epithelial morphogenesis, cell proliferation and gene expression.
authors.affiliation: Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine.
authors.forename: Lucia
authors.initials: L
authors.lastname: Speroni
authors.affiliation: Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Sweeney
authors.affiliation: Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sonnenschein
authors.affiliation: Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine; ana.soto@tufts.edu.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Soto
mesh_terms: D018929:Cell Culture Techniques; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology*