title: Bioanalytical method development for quantification of ulifloxacin, fenbufen and felbinac in rat plasma by solid-phase extraction (SPE) and HPLC with PDA detection.
abstract: A procedure based on solid-phase extraction (SPE) followed by high performance liquid chromatography (HPLC) with PDA detection has been developed for the analysis of multiple drugs in rat plasma. The analytes evaluated were ulifloxacin, fenbufen and felbinac. Eight different solid phase extraction cartridges were tested to evaluate their applicability for the isolation of drugs from rat plasma. Comparison were recovery of different drugs and reproducibility. The samples were analyzed by HPLC using a Kinetex C18 EVO column and acetonitrile-10mM ammonium acetate-methanol as the mobile phase under gradient elution conditions. SPE combined with HPLC-PDA allowed the determination of drugs over a linear range of 0.05-15 μg/mL for ulifloxacin while 0.5-50 μg/mL for felbinac and fenbufen, with limit of detection at 0.05 for ulifloxacin and 0.5 for felbinac and fenbufen. Bond Elut Plexa sorbent was found to provide the most effective clean-up, removing the greatest amount of interfering substance and simultaneously ensuring analyte recoveries higher than 93.54% with relative standard deviation (RSD) <10%. The method was applied with good accuracy and precision in the determination of ulifloxacin, fenbufen and felbinac in rat plasma obtained from rats treated with selected drugs. This method permits its application to pharmacokinetic and pharmacodynamic studies of these analytes and will facilitate detailed investigations on the interactions between new fluoroquinolones and fenbufen.
authors.affiliation: Dipartimento di Farmacia, Università degli Studi "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Ferrone
authors.affiliation: Dipartimento di Farmacia, Università degli Studi "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Carlucci
authors.affiliation: Dipartimento di Medicina Clinica, Sanità Pubblica, Scienze della Vita e dell'Ambiente, Università degli Studi di L'Aquila, 67100 L'Aquila, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Palumbo
authors.affiliation: Dipartimento di Farmacia, Università degli Studi "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy. Electronic address: giuseppe.carlucci@unich.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Carlucci
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones / Q000097:blood* / Q000737:chemistry; D057230:Limit of Detection; D010648:Phenylacetates / Q000097:blood* / Q000737:chemistry; D010654:Phenylbutyrates / Q000097:blood* / Q000737:chemistry; D010879:Piperazines / Q000097:blood* / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D024841:Fluoroquinolones; D010648:Phenylacetates; D010654:Phenylbutyrates; D010879:Piperazines; C012776:biphenylylacetic acid; C010725:fenbufen; C074190:ulifloxacin
keywords: Felbinac solid-phase extraction; Fenbufen; HPLC; Rat plasma; Ulifloxacin