title: Illumina MiSeq sequencing reveals the key microorganisms involved in partial nitritation followed by simultaneous sludge fermentation, denitrification and anammox process.
abstract: A combined process including a partial nitritation SBR (PN-SBR) followed by a simultaneous sludge fermentation, denitrification and anammox reactor (SFDA) was established to treat low C/N domestic wastewater in this study. An average nitrite accumulation rate of 97.8% and total nitrogen of 9.4mg/L in the effluent was achieved during 140days' operation. The underlying mechanisms were investigated by using Illumina MiSeq sequencing to analyze the microbial community structures in the PN-SBR and SFDA. Results showed that the predominant bacterial phylum was Proteobacteria in the external waste activated sludge (WAS, added to the SFDA) and SFDA while Bacteroidetes in the PN-SBR. Further study indicated that in the PN-SBR, the dominant nitrobacteria, Nitrosomonas genus, facilitated nitritation and little nitrate was generated in the PN-SBR effluent. In the SFDA, the co-existence of functional microorganisms Thauera, Candidatus Anammoximicrobium and Pseudomonas were found to contribute to simultaneous sludge fermentation, denitrification and anammox.
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address: pyz@bjut.edu.cn.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Mengyue
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
mesh_terms: D000693:Anaerobiosis; D041963:Bacteroidetes / Q000737:chemistry; D044822:Biodiversity; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D017404:In Situ Hybridization, Fluorescence; D009566:Nitrates / Q000737:chemistry; D009573:Nitrites / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D009606:Nitrosomonas / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000737:chemistry; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000737:chemistry*; D062065:Wastewater / Q000737:chemistry
chemical_list: D009566:Nitrates; D009573:Nitrites; D009584:Nitrogen; D012722:Sewage; D062065:Wastewater
keywords: Anammox; Denitrification; Fermentation; Microbial community; Partial nitritation