title: Experimental evidence for proteins constituting virion components and particle morphogenesis of bacteriophage ZF40.
abstract: Bacteriophage ZF40 is the only currently available, temperate Myoviridae phage infecting the potato pathogen Pectobacterium carotovorum subsp. carotovorum. Despite its unusual tail morphology, its major tail sheath and tube proteins remained uncharacterized after the initial genome annotation. Using ESI tandem mass-spectrometry, 24 structural proteins of the ZF40 virion were identified, with a sequence coverage ranging between 15.8% and 87.8%. The putative function of 16 proteins could be elucidated based on secondary structure analysis and conservative domain searches. The experimental annotation of 35% of the encoded gene products within the structural region of the genome represents a complete view of the virion structure, which can serve as the basis for future structural analysis as a model phage.
authors.affiliation: Department of Bacteriophage Molecular Genetics, D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, D03680 Kyiv, Ukraine natalia@korol.biz.
authors.forename: Natalia
authors.initials: N
authors.lastname: Korol
authors.affiliation: Laboratory of Gene Technology, Department of Biosystems, KU Leuven, 3001 Leuven, Belgium Division of Bacterial Diseases, Scientific Institute of Public Health (WIV-ISP), 1050 Brussels, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Van den Bossche
authors.affiliation: Department of Bacteriophage Molecular Genetics, D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, D03680 Kyiv, Ukraine.
authors.forename: Liudmyla
authors.initials: L
authors.lastname: Romaniuk
authors.affiliation: Biomedical Research Institute and Transnationale Universiteit Limburg, School of Life Sciences, Hasselt University, 3590 Diepenbeek, Belgium.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.affiliation: Laboratory of Gene Technology, Department of Biosystems, KU Leuven, 3001 Leuven, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Lavigne
authors.affiliation: Department of Bacteriophage Molecular Genetics, D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, D03680 Kyiv, Ukraine.
authors.forename: Fedor
authors.initials: F
authors.lastname: Tovkach
mesh_terms: D001435:Bacteriophages / Q000502:physiology* / Q000648:ultrastructure*; D036022:Capsid Proteins / Q000378:metabolism; D023061:Gene Order; D016679:Genome, Viral; D016366:Open Reading Frames; D020543:Proteome; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion
chemical_list: D036022:Capsid Proteins; D020543:Proteome; D014764:Viral Proteins
keywords: ESI-MS/MS; Pectobacterium carotovorum; bacteriophage ZF40; proteome analysis; virion morphogenesis