title: Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale.
abstract: Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis.
authors.affiliation: Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.
authors.forename: Manuel F
authors.identifier: http://orcid.org/0000-0002-8760-8080
authors.initials: MF
authors.lastname: Juette
authors.affiliation: Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.
authors.forename: Daniel S
authors.identifier: http://orcid.org/0000-0001-9021-0084
authors.initials: DS
authors.lastname: Terry
authors.affiliation: Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Wasserman
authors.affiliation: Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.
authors.forename: Roger B
authors.initials: RB
authors.lastname: Altman
authors.affiliation: Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Blanchard
mesh_terms: D000465:Algorithms; D001419:Bacteria / Q000648:ultrastructure; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D012343:RNA, Transfer / Q000648:ultrastructure*; D012270:Ribosomes / Q000648:ultrastructure*; D013997:Time Factors
chemical_list: D012343:RNA, Transfer