title: Mutielemental concentration and physiological responses of Lavandula pedunculata growing in soils developed on different mine wastes.
abstract: This study aimed to: i) evaluate the accumulation and translocation patterns of potentially hazardous elements into the Lavandula pedunculata and their influence in the concentrations of nutrients; and ii) compare some physiological responses associated with oxidative stress (concentration of chlorophylls (Chla, Chlb and total), carotenoids, and total protein) and several components involved in tolerance mechanisms (concentrations of proline and acid-soluble thiols and total/specific activity of catalase (CAT) and superoxide dismutase (SOD)), in plants growing in soils with a multielemental contamination and non-contaminated. Composite samples of soils, developed on mine wastes and/or host rocks, and L. pedunculata (roots and shoots) were collected in São Domingos mine (SE of Portugal) and in a reference area with non-contaminated soils, Corte do Pinto, with the same climatic conditions. São Domingos soils had high total concentrations of several hazardous elements (e.g. As and Pb) but their available fractions were small (mainly <5.8 % of the total). Translocation behaviour of elements was not clear according to the physiological importance of the elements. In general, plant shoots from São Domingos had the highest elements concentrations, but only As, Mn and Zn reached phytotoxic concentrations. Concentration of Chlb in shoots from São Domingos was higher than those from Corte do Pinto. No significant differences were obtained between concentrations of Chla, total protein, proline and acid-soluble thiols in shoots collected in both areas, as well as SOD activity (total and specific) and specific CAT activity. Total CAT activity varied with population being lower in the shoots of the plants from São Domingos, but no correlation was obtained between this enzymatic activity and the concentrations of the studied elements in shoots. Lavandula pedunculata plants are able to survive in soils developed on different mine wastes with multielemental contamination and low fertility showing no symptoms (visible and physiological) of phytotoxicity or deficiency.
authors.affiliation: Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017, Lisboa, Portugal; Centro de Investigação em Ciências do Ambiente e Empresariais (CICAE), Instituto Superior Dom Afonso III, Convento Espírito Santo, 8100-641, Loulé, Portugal. Electronic address: erika.santos@inuaf-studia.pt.
authors.forename: Erika S
authors.initials: ES
authors.lastname: Santos
authors.affiliation: Linking Landscape, Environment, Agriculture and Food (LEAF), Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017, Lisboa, Portugal. Electronic address: manuelaabreu@isa.ulisboa.pt.
authors.forename: Maria Manuela
authors.initials: MM
authors.lastname: Abreu
authors.affiliation: Research Unit of Organic Chemistry, Natural and Agro-food Products (QOPNA), Chemistry Department, Aveiro University, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal. Electronic address: jorgesaraiva@ua.pt.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Saraiva
mesh_terms: D027523:Lavandula / Q000254:growth & development* / Q000502:physiology; D008670:Metals / Q000032:analysis*; D008906:Mining; D018384:Oxidative Stress; D011174:Portugal; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis*; D013482:Superoxide Dismutase
chemical_list: D008670:Metals; D012987:Soil; D012989:Soil Pollutants; D013482:Superoxide Dismutase
keywords: Iberian Pyrite Belt; Mutielemental contamination; Physiological responses; São Domingos mine