title: Metabolic engineering of Klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production.
abstract: OBJECTIVE
  To improve the production of 2,3-butanediol (2,3-BD) in Klebsiella pneumoniae, the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated.
  
  
  RESULTS
  Although the cell growth and 2,3-BD production rates of the K. pneumoniae ΔldhA ΔadhE Δpta-ackA strain were lower than those of the wild-type strain, the mutant produced a higher titer of 2,3-BD and a higher yield in batch fermentation: 91 g 2,3-BD/l with a yield of 0.45 g per g glucose and a productivity of 1.62 g/l.h in fed-batch fermentation. The metabolic characteristics of the mutants were consistent with the results of in silico simulation.
  
  
  CONCLUSIONS
  K. pneumoniae knockout mutants developed with an aid of in silico investigation could produce higher amounts of 2,3-BD with increased titer, yield, and productivity.
authors.affiliation: Research and Development Center, GS Caltex Corporation, Expo-ro 359, Yusung-gu, Daejeon, 305-380, Republic of Korea.
authors.forename: Chelladurai
authors.initials: C
authors.lastname: Rathnasingh
authors.affiliation: Research and Development Center, GS Caltex Corporation, Expo-ro 359, Yusung-gu, Daejeon, 305-380, Republic of Korea.
authors.forename: Jong Myoung
authors.initials: JM
authors.lastname: Park
authors.affiliation: Research and Development Center, GS Caltex Corporation, Expo-ro 359, Yusung-gu, Daejeon, 305-380, Republic of Korea.|BioSystem Engineering Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Yusung-gu, Daejeon, 305-704, Republic of Korea.
authors.forename: Duk-Ki
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Research and Development Center, GS Caltex Corporation, Expo-ro 359, Yusung-gu, Daejeon, 305-380, Republic of Korea. hyohaks@gscaltex.com.
authors.forename: Hyohak
authors.initials: H
authors.lastname: Song
authors.affiliation: BioSystem Engineering Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Yusung-gu, Daejeon, 305-704, Republic of Korea.
authors.forename: Yong Keun
authors.initials: YK
authors.lastname: Chang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D002072:Butylene Glycols / Q000378:metabolism*; D003198:Computer Simulation; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D009154:Mutation
chemical_list: D001426:Bacterial Proteins; D002072:Butylene Glycols; C026978:2,3-butylene glycol
keywords: 2,3-Butanediol; Acetate kinase; Alcohol dehydrogenase; In silico investigation; Klebsiella pneumoniae; Lactate dehydrogenase; Phosphotransacetylase