title: A Preliminary Comparative Assessment of the Role of CD8+ T Cells in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis and Multiple Sclerosis.
abstract: Background. CD8+ T cells have putative roles in the regulation of adaptive immune responses during infection. The purpose of this paper is to compare the status of CD8+ T cells in Multiple Sclerosis (MS) and Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). Methods. This preliminary investigation comprised 23 CFS/ME patients, 11 untreated MS patients, and 30 nonfatigued controls. Whole blood samples were collected from participants, stained with monoclonal antibodies, and analysed on the flow cytometer. Using the following CD markers, CD27 and CD45RA (CD45 exon isoform 4), CD8+ T cells were divided into naïve, central memory (CM), effector memory CD45RA- (EM), and effector memory CD45RA+ (EMRA) cells. Results. Surface expressions of BTLA, CD127, and CD49/CD29 were increased on subsets of CD8+ T cells from MS patients. In the CFS/ME patients CD127 was significantly decreased on all subsets of CD8+ T cells in comparison to the nonfatigued controls. PSGL-1 was significantly reduced in the CFS/ME patients in comparison to the nonfatigued controls. Conclusions. The results suggest significant deficits in the expression of receptors and adhesion molecules on subsets of CD8+ T cells in both MS and CFS/ME patients. These deficits reported may contribute to the pathogenesis of these diseases. However, larger sample size is warranted to confirm and support these encouraging preliminary findings.
authors.affiliation: The National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Gold Coast, Australia.
authors.forename: Ekua W
authors.initials: EW
authors.lastname: Brenu
authors.affiliation: School of Medicine, Griffith University, Gold Coast, Australia.
authors.forename: Simon
authors.initials: S
authors.lastname: Broadley
authors.affiliation: The National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Gold Coast, Australia; School of Medical Science, Griffith University, Gold Coast, Australia.
authors.forename: Thao
authors.initials: T
authors.lastname: Nguyen
authors.affiliation: The National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Gold Coast, Australia; School of Medical Science, Griffith University, Gold Coast, Australia.
authors.forename: Samantha
authors.initials: S
authors.lastname: Johnston
authors.affiliation: The National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Gold Coast, Australia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ramos
authors.affiliation: The National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Gold Coast, Australia.
authors.forename: Don
authors.initials: D
authors.lastname: Staines
authors.affiliation: The National Centre for Neuroimmunology and Emerging Diseases, Griffith Health Institute, Griffith University, Gold Coast, Australia; School of Medical Science, Griffith University, Gold Coast, Australia.
authors.forename: Sonya
authors.initials: S
authors.lastname: Marshall-Gradisnik
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D016022:Case-Control Studies; D015673:Fatigue Syndrome, Chronic / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction
chemical_list: D015703:Antigens, CD; C482586:BTLA protein, human; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein; D011971:Receptors, Immunologic