title: Deciphering the biological effects of acupuncture treatment modulating multiple metabolism pathways.
abstract: Acupuncture is an alternative therapy that is widely used to treat various diseases. However, detailed biological interpretation of the acupuncture stimulations is limited. We here used metabolomics and proteomics technology, thereby identifying the serum small molecular metabolites into the effect and mechanism pathways of standardized acupuncture treatments at 'Zusanli' acupoint which was the most often used acupoint in previous reports. Comprehensive overview of serum metabolic profiles during acupuncture stimulation was investigated. Thirty-four differential metabolites were identified in serum metabolome and associated with ten metabolism pathways. Importantly, we have found that high impact glycerophospholipid metabolism, fatty acid metabolism, ether lipid metabolism were acutely perturbed by acupuncture stimulation. As such, these alterations may be useful to clarify the biological mechanism of acupuncture stimulation. A series of differentially expressed proteins were identified and such effects of acupuncture stimulation were found to play a role in transport, enzymatic activity, signaling pathway or receptor interaction. Pathway analysis further revealed that most of these proteins were found to play a pivotal role in the regulation of multiple metabolism pathways. It demonstrated that the metabolomics coupled with proteomics as a powerful approach for potential applications in understanding the biological effects of acupuncture stimulation.
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhang
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Yan
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Cheng
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Xiangcai
authors.initials: X
authors.lastname: Meng
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xie
authors.affiliation: National TCM Key Laboratory of Serum Pharmacochemistry, Sino-US Technology Cooperation Center of Chinmedomics, Research Center of Chinmedomics (State Administration of TCM), Laboratory of Metabolomics, Key Pharmacometabolomics Platform of Chinese Medicines, Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road, Harbin, China.
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
mesh_terms: D026881:Acupuncture; D015670:Acupuncture Therapy; D015415:Biomarkers; D019295:Computational Biology / Q000379:methods; D006801:Humans; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods
chemical_list: D015415:Biomarkers