title: Adaptation of the inflammatory immune response across pregnancy and postpartum in Black and White women.
abstract: Pregnancy is a period of considerable physiological adaption in neuroendocrine, cardiovascular, as well as immune function. Understanding of typical changes in inflammatory immune responses during healthy pregnancy is incomplete. In addition, despite considerable racial difference in adverse pregnancy outcomes, data are lacking on potential racial differences in such adaptation. This repeated measures prospective cohort study included 37 Black and 39 White women who provided blood samples during early, mid-, and late pregnancy and 8-10 weeks postpartum. Peripheral blood mononuclear cells were incubated with lipopolysaccharide (LPS) for 24h and supernatants assayed by electrochemiluminescence to quantify interleukin(IL)-6, tumor necrosis factor(TNF)-α, IL-1β, and IL-8 production. While no changes were observed in IL-8 production over time, significant increases in IL-6, TNF-α, and IL-1β production were observed from early to late pregnancy, with subsequent declines approaching early pregnancy values at postpartum (ps<0.05). Overall, inflammatory response patterns were highly similar among Black versus White women. However, Black women had greater TNF-α production during mid-pregnancy (p=0.002) and marginally lower IL-1β production at postpartum (p=0.054). These data show a clear trajectory of change in the inflammatory immune response across pregnancy and postpartum. In this cohort of generally healthy women, Black and White women exhibited minimal differences in LPS-stimulated cytokine production across the perinatal period. Future prospective studies in Black and White women with healthy versus adverse outcomes (e.g., preeclampsia, preterm birth) would inform our understanding of the potential role of immune dysregulation in pregnant women and in relation to racial disparities in perinatal health.
authors.affiliation: College of Nursing, The Ohio State University, Columbus, OH, United States.
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Gillespie
authors.affiliation: Center for Biostatistics, The Ohio State University, Columbus, OH, United States.
authors.forename: Kyle
authors.initials: K
authors.lastname: Porter
authors.affiliation: Department of Psychiatry, The Ohio State University Wexner Medical Center, Columbus, OH, United States; The Institute for Behavioral Medicine Research, The Ohio State University Wexner Medical Center, Columbus, OH, United States; Department of Obstetrics and Gynecology, The Ohio State University Wexner Medical Center, Columbus, OH, United States. Electronic address: Lisa.Christian@osumc.edu.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Christian
mesh_terms: D000328:Adult; D001741:Black or African American; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood / Q000276:immunology*; D049590:Postpartum Period / Q000097:blood / Q000276:immunology*; D011247:Pregnancy / Q000097:blood / Q000276:immunology*; D044465:White People
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators
keywords: Cytokines; Immunology; Inflammation; Lipopolysaccharide; Postpartum; Pregnancy; Race; Racial disparities; Stimulated cytokine production