title: Large genomic differences between Moraxella bovoculi isolates acquired from the eyes of cattle with infectious bovine keratoconjunctivitis versus the deep nasopharynx of asymptomatic cattle.
abstract: Moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (IBK) or "pinkeye" in cattle. In this study, closed circularized genomes were generated for seven M. bovoculi isolates: three that originated from the eyes of clinical IBK bovine cases and four from the deep nasopharynx of asymptomatic cattle. Isolates that originated from the eyes of IBK cases profoundly differed from those that originated from the nasopharynx of asymptomatic cattle in genome structure, gene content and polymorphism diversity and consequently placed into two distinct phylogenetic groups. These results suggest that there are genetically distinct strains of M. bovoculi that may not associate with IBK.
authors.affiliation: United States Department of Agriculture (USDA), Agricultural Research Service (ARS), U.S. Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, NE, 68933, USA. aaron.dickey@ars.usda.gov.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Dickey
authors.affiliation: School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583-0907, USA. jdloy@unl.edu.
authors.forename: John D
authors.initials: JD
authors.lastname: Loy
authors.affiliation: United States Department of Agriculture (USDA), Agricultural Research Service (ARS), U.S. Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, NE, 68933, USA. jim.bono@ars.usda.gov.
authors.forename: James L
authors.initials: JL
authors.lastname: Bono
authors.affiliation: United States Department of Agriculture (USDA), Agricultural Research Service (ARS), U.S. Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, NE, 68933, USA. tim.smith@ars.usda.gov.
authors.forename: Timothy P L
authors.initials: TP
authors.lastname: Smith
authors.affiliation: College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. mapley@vet.k-state.edu.
authors.forename: Mike D
authors.initials: MD
authors.lastname: Apley
authors.affiliation: Kansas State Diagnostic Laboratory, Kansas State University, Manhattan, KS, 66506, USA. blubbers@k-state.edu.
authors.forename: Brian V
authors.initials: BV
authors.lastname: Lubbers
authors.affiliation: College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. kdd5257@vet.k-state.edu.
authors.forename: Keith D
authors.initials: KD
authors.lastname: DeDonder
authors.affiliation: College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. scapik@vet.k-state.edu.
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Capik
authors.affiliation: College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. rlarson@vet.k-state.edu.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Larson
authors.affiliation: College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66506, USA. bwhite@vet.k-state.edu.
authors.forename: Brad J
authors.initials: BJ
authors.lastname: White
authors.affiliation: Bioinformatics and Systems Biology, Justus-Liebig-University Giessen, 35392, Giessen, Germany. Jochen.Blom@computational.bio.uni-giessen.de.
authors.forename: Jochen
authors.initials: J
authors.lastname: Blom
authors.affiliation: United States Department of Agriculture (USDA), Agricultural Research Service (ARS), U.S. Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, NE, 68933, USA. carol.chitkomckown@ars.usda.gov.
authors.forename: Carol G
authors.initials: CG
authors.lastname: Chitko-McKown
authors.affiliation: United States Department of Agriculture (USDA), Agricultural Research Service (ARS), U.S. Meat Animal Research Center (USMARC), State Spur 18D, Clay Center, NE, 68933, USA. mike.clawson@ars.usda.gov.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Clawson
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005123:Eye / Q000382:microbiology; D007637:Keratoconjunctivitis / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009016:Moraxella / Q000235:genetics*; D045828:Moraxellaceae Infections / Q000382:microbiology / Q000662:veterinary*; D009305:Nasopharynx / Q000382:microbiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D001426:Bacterial Proteins