title: Ultrasound triggered image-guided drug delivery to inhibit vascular reconstruction via paclitaxel-loaded microbubbles.
abstract: Paclitaxel (PTX) has been recognized as a promising drug for intervention of vascular reconstructions. However, it is still difficult to achieve local drug delivery in a spatio-temporally controllable manner under real-time image guidance. Here, we introduce an ultrasound (US) triggered image-guided drug delivery approach to inhibit vascular reconstruction via paclitaxel (PTX)-loaded microbubbles (PLM) in a rabbit iliac balloon injury model. PLM was prepared through encapsulating PTX in the shell of lipid microbubbles via film hydration and mechanical vibration technique. Our results showed PLM could effectively deliver PTX when exposed to US irradiation and result in significantly lower viability of vascular smooth muscle cells. Ultrasonographic examinations revealed the US signals from PLM in the iliac artery were greatly increased after intravenous administration of PLM, making it possible to identify the restenosis regions of iliac artery. The in vivo anti-restenosis experiments with PLM and US greatly inhibited neointimal hyperplasia at the injured site, showing an increased lumen area and reduced the ratio of intima area and the media area (I/M ratio). No obvious functional damages to liver and kidney were observed for those animals. Our study provided a promising approach to realize US triggered image-guided PTX delivery for therapeutic applications against iliac restenosis.
authors.affiliation: Department of Cardiology, Children's Hospital of Chongqing Medical University, Chongqing, China.|Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, Children's Hospital of Chongqing Medical University, Chongqing, China.|Department of Pediatrics, Mianyang Central Hospital, Mianyang, Sichuan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Cardiology, Children's Hospital of Chongqing Medical University, Chongqing, China.|Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
authors.forename: Cancan
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Cardiology, Children's Hospital of Chongqing Medical University, Chongqing, China.|Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
authors.forename: Huaxiao
authors.initials: H
authors.lastname: Geng
authors.affiliation: Department of Cardiology, Children's Hospital of Chongqing Medical University, Chongqing, China.|Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
authors.forename: Gengsheng
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Radiology, 324 Hospital of the PLA, Chongqing, China.
authors.forename: Jinqing
authors.initials: J
authors.lastname: Li
authors.affiliation: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.|Shenzhen Key Laboratory of Nanobiomechanics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Cardiology, Children's Hospital of Chongqing Medical University, Chongqing, China.|Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Ji
authors.affiliation: Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D003251:Constriction, Pathologic / Q000473:pathology / Q000628:therapy*; D004339:Drug Compounding; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D007083:Iliac Artery / Q000187:drug effects* / Q000473:pathology; D045423:Microbubbles; D009131:Muscle, Smooth, Vascular / Q000528:radiation effects; D032389:Myocytes, Smooth Muscle / Q000528:radiation effects; D017239:Paclitaxel / Q000494:pharmacology*; D011817:Rabbits; D000069453:Ultrasonic Waves
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel