title: Wash-free magnetic immunoassay of the PSA cancer marker using SERS and droplet microfluidics.
abstract: We report a novel wash-free magnetic immunoassay technique for prostate-specific antigen (PSA) that uses a surface-enhanced Raman scattering (SERS)-based microdroplet sensor. The magnetic bar embedded in a droplet-based microfluidic system segregates the free and bound SERS tags by splitting the droplets into two smaller parts. The presence of PSA targets leads more SERS tags to immunocomplex in one droplet so that fewer SERS tags remain in another supernatant solution droplet. Thus, SERS signal measurement enables the quantitative evaluation of PSA markers. This approach can provide a rapid and sensitive assay that is applicable for PSA cancer markers in serum without any washing. Specifically, SERS signals were measured at 174 droplets per minute and averaged for quantitative evaluation of PSA. The limit of detection (LOD) determined by our SERS-based microdroplet sensor was estimated to be below 0.1 ng mL(-1), which is significantly below the clinical cut-off value for the diagnosis of prostate cancer. In addition, because the entire assay can be carried out automatically, only a minimal amount of sample is needed. Accordingly, the approach is expected to be useful as a potential clinical tool for the early diagnosis of prostate cancer.
authors.affiliation: Department of Bionano Technology, Hanyang University, Ansan 426-791, South Korea. jbchoo@hanyang.ac.kr.
authors.forename: Rongke
authors.initials: R
authors.lastname: Gao
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Cheng
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: deMello
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D055088:Early Detection of Cancer; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D060328:Magnetic Phenomena; D059346:Magnets; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D010316:Particle Size; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000276:immunology; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation; D013499:Surface Properties
chemical_list: D014408:Biomarkers, Tumor; D017430:Prostate-Specific Antigen