title: Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis.
abstract: Agarwood, a highly valuable resinous and fragrant heartwood of Aquilaria plants, is widely used in traditional medicines, incense and perfume. Only when Aquilaria trees are wounded by external stimuli do they form agarwood sesquiterpene defensive compounds. Therefore, understanding the signaling pathway of wound-induced agarwood formation is important. Jasmonic acid (JA) is a well-characterized molecule that mediates a plant's defense response and secondary metabolism. However, little is known about the function of endogenous JA in agarwood sesquiterpene biosynthesis. Here, we report that heat shock can up-regulate the expression of genes in JA signaling pathway, induce JA production and the accumulation of agarwood sesquiterpene in A. sinensis cell suspension cultures. A specific inhibitor of JA, nordihydroguaiaretic acid (NDGA), could block the JA signaling pathway and reduce the accumulation of sesquiterpene compounds. Additionally, compared to SA and H2O2, exogenously supplied methyl jasmonate has the strongest stimulation effect on the production of sesquiterpene compounds. These results clearly demonstrate the central induction role of JA in heat-shock-induced sesquiterpene production in A. sinensis.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Xu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Yong-Cui
authors.initials: YC
authors.lastname: Liao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.|Hainan Branch Institute of Medicinal Plant, Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Chinese Academy of Medical Sciences &Peking Union Medical College, Wanning 571533, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Sun
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Gao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Sui
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.|Hainan Branch Institute of Medicinal Plant, Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Chinese Academy of Medical Sciences &Peking Union Medical College, Wanning 571533, China.
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Wei
mesh_terms: D000085:Acetates / Q000494:pharmacology; D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects / Q000235:genetics; D006358:Hot Temperature; D009637:Masoprocol / Q000494:pharmacology; D054883:Oxylipins / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D064210:Secondary Metabolism; D012717:Sesquiterpenes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction; D029645:Thymelaeaceae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D006360:Heat-Shock Proteins; D054883:Oxylipins; D010940:Plant Proteins; D012717:Sesquiterpenes; C011006:jasmonic acid; D009637:Masoprocol; C072239:methyl jasmonate