title: Sequential process in brain-derived neurotrophic factor-induced functional periodontal tissue regeneration.
abstract: We recently demonstrated that brain-derived neurotrophic factor (BDNF) promotes periodontal tissue regeneration. The purpose of this study was to establish an essential component of a rational approach for the clinical application of BDNF in periodontal regenerative therapy. Here, we assessed the sequence of early events in BDNF-induced periodontal tissue regeneration, especially from the aspect of cementum regeneration. Brain-derived neurotrophic factor was applied into experimental periodontal defects in Beagle dogs. The localization of cells positive for neurotrophic tyrosine kinase, receptor, type 2, proliferating cell nuclear antigen, osteopontin, integrin αVβ3, and integrin α2β1 was evaluated by immunohistochemistry. The effects of BDNF on adhesion of cultured human periodontal ligament cells was examined by an in vitro study. The results suggest that BDNF could induce rapid cementum regeneration by stimulating adhesion, proliferation, and differentiation of periodontal ligament cells in the early regenerative phase, resulting in enhancement of periodontal tissue regeneration.
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Konishi
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Takeda
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Fujita
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Mikihito
authors.initials: M
authors.lastname: Kajiya
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Matsuda
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kittaka
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Shiba
authors.affiliation: Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D003739:Dental Cementum; D004285:Dogs; D006801:Humans; D010513:Periodontal Ligament; D010519:Periodontium; D012038:Regeneration
chemical_list: D019208:Brain-Derived Neurotrophic Factor
keywords: brain-derived neurotrophic factor; periodontal ligament cells; periodontal tissue regeneration