title: Application of a novel phosphinothricin N-acetyltransferase (RePAT) gene in developing glufosinate-resistant rice.
abstract: Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated RePAT gene into a japonica rice variety Zhonghua11, resulting in a large number of independent T0 transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected. Agronomic performances of their T2 progenies were investigated, and the results suggested that the expression of RePAT had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR. The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene RePAT to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cui
authors.affiliation: National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Ziduo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000613:Aminobutyrates / Q000494:pharmacology*; D018628:Gene Dosage; D015870:Gene Expression; D005838:Genotype; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology*; D012275:Oryza / Q000187:drug effects* / Q000235:genetics*; D030821:Plants, Genetically Modified
chemical_list: D000613:Aminobutyrates; D006540:Herbicides; C003121:phosphinothricin; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase