title: The roasting process does not influence the extent of conjugation of coffee chlorogenic and phenolic acids.
abstract: Understanding the bioavailability and metabolism of coffee compounds will contribute to identify the unknown biological mechanism(s) linked to their beneficial effects. The influence of the roasting process on the metabolism of coffee chlorogenic acids in humans was evaluated. In a randomized, double-blind, crossover study, 12 healthy volunteers consumed four instant coffees namely, high roasted coffee (HRC), low roasted coffee (LRC), unroasted coffee (URC), and in vitro hydrolyzed unroasted coffee (HURC). The sum of areas under the curve (AUC) ranged from 8.65-17.6 to 30.9-126 µM/h (P < 0.05) for HRC, LRC, URC, and HURC, respectively. The AUC of HRC, LRC, and URC was correlated with the initial level of phenolic acids in the coffee drinks. Despite different absorption rates, the extent of conjugation was comparable between HRC, LRC, and URC coffees but different for HURC. The most abundant circulating metabolites during the first 5 H were dihydroferulic acid (DHFA), caffeic acid-3'-O-sulfate (CA3S) and isoferulic-3'-O-glucuronide (iFA3G). DHFA and 5-4-dihydro-m-coumaric acid (mDHCoA) were the main metabolites in the period of 5-24 H. The phenolic compounds after consumption of HURC were most rapidly absorbed (Tmax 1 H) compared with the other coffees (Tmax between 9 and 11 H). Using coffees with different degrees of roasting we highlighted that in spite of different absorption rates the extent of conjugation of phenolic acids was comparable. In addition, by using a hydrolyzed unroasted coffee we demonstrated an increased absorption of phenolic acids in the small intestine. © 2016 BioFactors, 42(3):259-267, 2016.
authors.affiliation: Nestlé Research Center, Nestec Ltd., Vers-Chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Belén
authors.initials: B
authors.lastname: Sanchez-Bridge
authors.affiliation: Nestlé Research Center, Nestec Ltd., Vers-Chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Renouf
authors.affiliation: Nestlé Research Center, Nestec Ltd., Vers-Chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Julien
authors.initials: J
authors.lastname: Sauser
authors.affiliation: Nestlé Research Center, Nestec Ltd., Vers-Chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Maurice
authors.initials: M
authors.lastname: Beaumont
authors.affiliation: Nestlé Research Center, Nestec Ltd., Vers-Chez-les-Blanc, Lausanne, Switzerland.
authors.forename: Lucas
authors.initials: L
authors.lastname: Actis-Goretta
mesh_terms: D001682:Biological Availability; D002109:Caffeic Acids / Q000737:chemistry / Q000378:metabolism; D002726:Chlorogenic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D003069:Coffee; D064368:Healthy Volunteers; D006801:Humans; D006868:Hydrolysis; D062385:Hydroxybenzoates / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D003069:Coffee; D062385:Hydroxybenzoates; D002726:Chlorogenic Acid; C017616:phenolic acid; C040048:caffeic acid
keywords: bioavailability; coffee; human; metabolism; phenolic acids; roasting