title: Autoantigen-targeting microRNAs in Sjögren's syndrome.
abstract: MicroRNAs are short endogenous non-coding RNAs that regulate gene expression in various physiological and pathological conditions. To characterize autoantigen-targeting microRNAs in Sjögren's syndrome (SS), a systematic study was carried out, in which a candidate microRNA set was first identified by bioinformatics analysis and literature search. Then, their gene silencing activities were evaluated with fusion reporter gene and endogenous targets, leading to the identification of three microRNAs: TRIM21-targeting miR-1207-5p, TRIM21-targeting miR-4695-3p, and La autoantigen-targeting miR-299-5p. Compared to healthy controls, downregulation of miR-1207-5p and miR-4695-3p expression was further revealed in the minor salivary glands of primary SS (pSS) patients. This, on the one hand, characterized two autoantigen-targeting microRNAs in Sjögren's syndrome and, on the other hand, suggested that downregulation of miR-1207-5p and miR-4695-3p expression may lead to increased TRIM21 levels in the minor salivary glands, which contributes to the development of Sjögren's syndrome.
authors.affiliation: Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China.
authors.forename: Linyi
authors.initials: L
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100871, China.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100871, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yi
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100871, China.
authors.forename: Zhenxi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, Sino-Japan Friendship Hospital, Beijing, 100029, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China.
authors.forename: Li Dan
authors.initials: LD
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100005, China. zhangfccra@yahoo.com.cn.
authors.forename: Fengchun
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, 100871, China. quan.du@pku.edu.cn.|Biomics Biotechnologies Co., Ltd., Nantong, 226016, China. quan.du@pku.edu.cn.|Peking University, Beijing, 100191, China. quan.du@pku.edu.cn.
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
mesh_terms: D000368:Aged; D001324:Autoantigens / Q000737:chemistry*; D001402:B-Lymphocytes / Q000276:immunology; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020868:Gene Silencing; D018390:Gene Targeting; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D012261:Ribonucleoproteins / Q000235:genetics; D012469:Salivary Glands / Q000378:metabolism; D012859:Sjogren's Syndrome / Q000235:genetics* / Q000276:immunology*; D000099207:SS-A Antigen
chemical_list: D001324:Autoantigens; D008156:Luciferases; D035683:MicroRNAs; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C575047:MIRN1207 microRNA, human; C553452:MIRN299 microRNA, human; C000613470:MIRN4695 microRNA, human
keywords: Autoantigen; B cell; MicroRNA; Sjögren’s syndrome