title: Highly sensitive electrochemical biosensor based on nonlinear hybridization chain reaction for DNA detection.
abstract: In the present work we demonstrated an ultrasensitive detection platform for specific DNA based on nonlinear hybridization chain reaction (HCR) by triggering chain-branching growth of DNA dendrimers. HCR was initiated by target DNA (tDNA) and finally formed dendritic structure by self-assembly. The electrochemical signal was drastically enhanced by capturing multiple catalytic peroxidase with high-ordered growth. Electrochemical signals were obtained by measuring the reduction current of oxidized 3, 3', 5, 5'-tetramethylbenzidine sulfate (TMB), which was generated by HRP in the presence of H2O2. This method exhibited ultrahigh sensitivity to tDNA with detection limit of 0.4 fM. Furthermore, the biosensor was also capable of discriminating single-nucleotide difference among concomitant DNA sequences.
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
authors.forename: Rongna
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Jia
authors.affiliation: Department of Chemistry, Liaocheng University, Liaocheng 252059, China. Electronic address: hswang@lcu.edu.cn.
authors.forename: Huaisheng
authors.initials: H
authors.lastname: Wang
mesh_terms: D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods*; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010084:Oxidation-Reduction
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D004247:DNA; D006861:Hydrogen Peroxide
keywords: Biosensor; DNA; Nonlinear hybridization chain reaction