title: Evaluation of the genotoxicity/mutagenicity and antigenotoxicity/antimutagenicity induced by propolis and Baccharis dracunculifolia, by in vitro study with HTC cells.
abstract: The ethanolic extract of propolis, especially the Brazilian green type, is widely and mainly used for therapeutic purposes despite the lack of knowledge about its effects and its cellular mode of action. This type of propolis, derived from Baccharis dracunculifolia (alecrim-do-campo), has been extensively commercialized and the consumers use it to enhance health. This work aimed to assess the genotoxic/mutagenic and antigenotoxic/antimutagenic potentials of the ethanolic extracts of Brazilian green propolis and of B. dracunculifolia, on mammalian cells. It was not observed genotoxic and mutagenic effects by both extracts. After evaluate the exposure of the cells to each extract with a recognized mutagen, simultaneously, the results showed a significant reduction on DNA damage. The experiment carried out with a pre-incubation period was more effective than without incubation test, showing that the tested extracts were able to inactivate the mutagen before it could react with the DNA.
authors.affiliation: Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil. Electronic address: mmr@rc.unesp.br.
authors.forename: Matheus Mantuanelli
authors.initials: MM
authors.lastname: Roberto
authors.affiliation: Departamento de Ciências Biológicas, Centro de Ciências Humanas e Naturais, UFES - Universidade Federal do Espírito Santo, Avenida Fernando Ferrari, 514, Goiabeiras, 29075-910 Vitória, ES, Brazil.
authors.forename: Sílvia Tamie
authors.initials: ST
authors.lastname: Matsumoto
authors.affiliation: Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, UFES - Universidade Federal do Espírito Santo, Avenida Marechal Campos, 1468, Maruípe, 29040-090 Vitória, ES, Brazil.
authors.forename: Cláudia Masrouah
authors.initials: CM
authors.lastname: Jamal
authors.affiliation: Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil.
authors.forename: Osmar
authors.initials: O
authors.lastname: Malaspina
authors.affiliation: Departamento de Biologia, Instituto de Biociências, UNESP - Univ. Estadual Paulista, Avenida 24-A, 1515, CP199, 13506-900 Rio Claro, SP, Brazil. Electronic address: mamm@rc.unesp.br.
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Marin-Morales
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D031175:Baccharis; D045744:Cell Line, Tumor; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D015162:Micronucleus Tests; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011429:Propolis / Q000494:pharmacology*; D051381:Rats
chemical_list: D016587:Antimutagenic Agents; D010936:Plant Extracts; D011429:Propolis
keywords: Brazilian green propolis; Caffeic acid (PubChem CID: 689043); Comet assay; Cytokinesis-blockage micronucleus test; Ethanolic extract; Ferulic acid (PubChem CID: 445858); Kaempferol (PubChem CID: 5280863); Methyl methanesulfonate (PubChem CID: 4156); Mutagen inactivation; Quercetin (PubChem CID: 5280343); Rutin (PubChem CID: 5280805)