title: Preparation and characterization of crosslinked chitosan/gelatin scaffolds by ice segregation induced self-assembly.
abstract: Chitosan and gelatin are biodegradable and biocompatible polymers which may be used in the preparation of 3D scaffolds with applications in biomedicine. Chitosan/gelatin scaffolds crosslinked with glutaraldehyde were prepared by ice segregation induced self-assembly (ISISA); a unidirectional freezing at -196°C followed freeze-drying to produce macroporous materials with a well-patterned structure. This process may be included within the green chemistry by the preparation of the porous structures without using organic solvents, moreover is a versatile, non-difficult and cheap process. The scaffolds prepared by ISISA were characterized by scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy, thermal gravimetric analysis, differential scanning calorimetry, and their stability was evaluated by degree swelling and degradation tests. The scaffolds present properties as high porosity, high degree swelling and good stability which make them suitable of applications as biomaterials.
authors.affiliation: Center for Research in Biological Chemistry and Molecular Materials (CIQUS), University of Santiago de Compostela, 15782 Santiago de Compostela, Spain; Department of Physical Chemistry, Faculty of Chemistry, University of Santiago de Compostela (USC), 15782 Santiago de Compostela, Spain. Electronic address: m.nieto@usc.es.
authors.forename: Marina
authors.initials: M
authors.lastname: Nieto-Suárez
authors.affiliation: Department of Physical Chemistry, Faculty of Chemistry, University of Santiago de Compostela (USC), 15782 Santiago de Compostela, Spain.
authors.forename: M Arturo
authors.initials: MA
authors.lastname: López-Quintela
authors.affiliation: Center for Research in Biological Chemistry and Molecular Materials (CIQUS), University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Massimo
authors.initials: M
authors.lastname: Lazzari
mesh_terms: D055162:Biocatalysis; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D005615:Freezing; D005780:Gelatin / Q000737:chemistry*; D055772:Green Chemistry Technology; D020100:Hydrogels / Q000138:chemical synthesis* / Q000737:chemistry; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D009113:Muramidase / Q000378:metabolism; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D005780:Gelatin; D048271:Chitosan; D009113:Muramidase
keywords: Biomaterial; Chitosan; Gelatin; Hydrogel; Scaffold; Unidirectional freezing