title: A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins.
abstract: A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal antibodies were selected for this assay. The semi-quantitative results were obtained within 20 min by the naked eye, with visual limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.1-0.5, 2.5-250, 0.5-1, 0.25-1 and 2.5-10 μg kg(-1), and cut-off values of 0.25-1, 5-500, 1-10, 0.5-2.5 and 5-25 μg kg(-1), respectively. The quantitative results were obtained using a hand-held strip scan reader, with the calculated limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.04-0.17, 0.06-49, 0.15-0.22, 0.056-0.49 and 0.53-1.05 μg kg(-1), respectively. The analytical results of spiked samples were in accordance with the accurate content in the simultaneous detection analysis. This newly developed ICA strip assay is suitable for the on-site detection and rapid initial screening of mycotoxins in cereal samples, facilitating both semi-quantitative and quantitative determination.
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China. xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
authors.forename: Dezhao
authors.initials: D
authors.lastname: Kong
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China. xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China. xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Song
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China. xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
authors.forename: Steven
authors.initials: S
authors.lastname: Suryoprabowo
authors.affiliation: Cereals & Oils Nutrition Research Group, Academy of Science & Technology of State Administration of Grain, Beijing 100037, People's Republic of China. lak@chinagrain.org.
authors.forename: Aike
authors.initials: A
authors.lastname: Li
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China. xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China. xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China. xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D009183:Mycotoxins / Q000032:analysis*; D010209:Paper
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D009183:Mycotoxins; D006046:Gold