title: Transcriptome-wide identification and expression analysis of chrysanthemum SBP-like transcription factors.
abstract: SQUAMOSA promoter-binding protein (SBP) transcription factors are known to function in a number of processes in plants. Here, we have characterized twelve SBP-like (SPL) genes in the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of twelve distinct sequences were isolated and amplified based on transcriptomic sequences. Phylogenetic analysis identified two pairs of orthologous proteins for Arabidopsis and chrysanthemum and two pairs of paralogous proteins in chrysanthemum. Conserved motifs in the SPL proteins shared by Arabidopsis and chrysanthemum were scanned using MEME. A bioinformatics analysis revealed that six of these genes contained a miR156 target site, while five CmSPLs were targeted by miR157. Moreover, we used 5' RLM-RACE to map the cleavage sites in CmSPL2 and CmSPL3. The expression of these twelve genes in response to a variety of phytohormone treatments and abiotic stresses was characterized. This work improves our understanding of the various functions of SPL gene family members in the stress response.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing, 210095, China. Electronic address: aiping_song@aliyun.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 2014104101@njau.edu.cn.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Gao
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 2013104120@njau.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 2014104098@njau.edu.cn.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xin
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: guanzhy@njau.edu.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: hb@njau.edu.cn.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 14112317@njau.edu.cn.
authors.forename: Lili
authors.initials: L
authors.lastname: Jin
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing, 210095, China. Electronic address: chenfd@njau.edu.cn.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D031202:Chrysanthemum / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D059467:Transcriptome / Q000502:physiology*
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors
keywords: Chrysanthemum morifolium; Phylogenetic analysis; SBP-box; Stress response; Transcription pattern