title: Induction of site-specific chromosomal translocations in embryonic stem cells by CRISPR/Cas9.
abstract: Chromosomal translocation is the most common form of chromosomal abnormality and is often associated with congenital genetic disorders, infertility, and cancers. The lack of cellular and animal models for chromosomal translocations, however, has hampered our ability to understand the underlying disease mechanisms and to develop new therapies. Here, we show that site-specific chromosomal translocations can be generated in mouse embryonic stem cells (mESCs) via CRISPR/Cas9. Mouse ESCs carrying translocated chromosomes can be isolated and expanded to establish stable cell lines. Furthermore, chimeric mice can be generated by injecting these mESCs into host blastocysts. The establishment of ESC-based cellular and animal models of chromosomal translocation by CRISPR/Cas9 provides a powerful platform for understanding the effect of chromosomal translocation and for the development of new therapeutic strategies.
authors.affiliation: Research Center of Developmental Biology, Histology and Embryology Department, Second Military Medical University, Shanghai, 200433, China.|Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.|Translational medicine center, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Guanyi
authors.initials: G
authors.lastname: Huang
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Fengsheng
authors.initials: F
authors.lastname: Li
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Ruizhe
authors.initials: R
authors.lastname: Wang
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Wu
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Youzhen
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Chang
authors.initials: C
authors.lastname: Tong
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Sankalp
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Research Center of Developmental Biology, Histology and Embryology Department, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Center of Developmental Biology, Histology and Embryology Department, Second Military Medical University, Shanghai, 200433, China.|Translational medicine center, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Houqi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Stem Cell Biology &Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Qi-Long
authors.initials: QL
authors.lastname: Ying
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D008957:Models, Genetic; D014178:Translocation, Genetic