title: Novel redox species polyaniline derivative-Au/Pt as sensing platform for label-free electrochemical immunoassay of carbohydrate antigen 199.
abstract: A novel electrochemical redox-active nanocomposite was synthesized by a one-pot method using N,N'-diphenyl-p-phenylediamine as monomer, and HAuCl4 and K2PtCl4 as co-oxidizing agents. The as-prepared poly(N,N'-diphenyl-p-phenylediamine)-Au/Pt exhibited admirable electrochemical redox activity at 0.15 V, excellent H2O2 electrocatalytic ability and favorable electron transfer ability. Based on these, the evaluation of the composite as sensing substrate for label-free electrochemical immunosensing to the sensitive detection of carbohydrate antigen 199 was described. This technique proved to be a prospective detection tool with a wide liner range from 0.001 U mL(-1) to 40 U mL(-1), and a low detection limit of 2.3 × 10(-4) U mL(-1) (S/N = 3). In addition, this method was used for the analysis of human serum sample, and good agreement was obtained between the values and those of enzyme-linked immunosorbent assay, implying the potential application in clinical research. Importantly, the strategy of the present substrate could be extended to other polymer-based nanocomposites such as polypyrrole derivatives or polythiophene derivatives, and this could be of great significance for the electrochemical immunoassay.
authors.affiliation: Department of Chemistry, Capital Normal University, 100048, Beijing, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Capital Normal University, 100048, Beijing, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Shan
authors.affiliation: Department of Chemistry, Capital Normal University, 100048, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.affiliation: Department of Chemistry, Capital Normal University, 100048, Beijing, China. Electronic address: mazhanfang@cnu.edu.cn.
authors.forename: Zhanfang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000814:Aniline Compounds; D015295:Antigens, Tumor-Associated, Carbohydrate; C578959:carbohydrate antigen 199, human; C416807:polyaniline; D010984:Platinum; D006046:Gold
keywords: Cancer biomarker; Electrochemical immunosensor; Electrochemical redox species; Label-free; Polyaniline derivative-Au/Pt; Sensing substrate