title: Inhibition of Toxic IAPP Amyloid by Extracts of Common Fruits.
abstract: The aggregation of the 37-amino acid polypeptide islet amyloid polypeptide (IAPP, amylin), as either insoluble amyloid or as small oligomers, appears to play a direct role in the death of pancreatic β-islet cells in type 2 diabetes. It is believed that inhibiting the aggregation of IAPP may slow down, if not prevent entirely, the progression of this disease. Extracts of thirteen different common fruits were analyzed for their ability to prevent the aggregation of amyloidogenic IAPP. Thioflavin T binding, immuno-detection and circular dichroism assays were performed to test thein vitroinhibitory potential of each extract. Atomic force microscopy was used to visualize the formation of amyloid fibrils with and without each fruit extract. Finally, extracts were tested for their ability to protect living mammalian cells from the toxic effects of amyloid IAPP. Several fruits showed substantial ability to inhibit IAPP aggregation and protect living cells from toxic IAPP amyloid.
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Kao
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: Evangeline
authors.initials: E
authors.lastname: Green
authors.affiliation: Department of Biology, Mount Saint Mary's College, 12001 Chalon Drive, Los Angeles, CA 90049.
authors.forename: Catalina
authors.initials: C
authors.lastname: Pereira
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: Shauna
authors.initials: S
authors.lastname: Ekimura
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: Dennis
authors.initials: D
authors.lastname: Juarez
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: Travis
authors.initials: T
authors.lastname: Whyte
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: Taylor
authors.initials: T
authors.lastname: Arhar
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: Bianca
authors.initials: B
authors.lastname: Malaspina
authors.affiliation: Department of Biology, Mount Saint Mary's College, 12001 Chalon Drive, Los Angeles, CA 90049.
authors.forename: Luiza A
authors.initials: LA
authors.lastname: Nogaj
authors.affiliation: Department of Chemistry and Biochemistry, Loyola Marymount University, 1 LMU Drive, Los Angeles, CA 90045.
authors.forename: David A
authors.initials: DA
authors.lastname: Moffet
keywords: Amyloid Inhibition; Diabetes; Natural Product Therapeutics