title: Comparative Proteomics Provides Insights into Metabolic Responses in Rat Liver to Isolated Soy and Meat Proteins.
abstract: It has been reported that isolated dietary soy and meat proteins have distinct effects on physiology and liver gene expression, but the impact on protein expression responses are unknown. Because these may differ from gene expression responses, we investigated dietary protein-induced changes in liver proteome. Rats were fed for 1 week semisynthetic diets that differed only regarding protein source; casein (reference) was fully replaced by isolated soy, chicken, fish, or pork protein. Changes in liver proteome were measured by iTRAQ labeling and LC-ESI-MS/MS. A robust set totaling 1437 unique proteins was identified and subjected to differential protein analysis and biological interpretation. Compared with casein, all other protein sources reduced the abundance of proteins involved in fatty acid metabolism and Pparα signaling pathway. All dietary proteins, except chicken, increased oxidoreductive transformation reactions but reduced energy and essential amino acid metabolic pathways. Only soy protein increased the metabolism of sulfur-containing and nonessential amino acids. Soy and fish proteins increased translation and mRNA processing, whereas only chicken protein increased TCA cycle but reduced immune responses. These findings were partially in line with previously reported transcriptome results. This study further shows the distinct effects of soy and meat proteins on liver metabolism in rats.
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiang Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Shangxin
authors.initials: S
authors.lastname: Song
authors.affiliation: Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University , Wageningen 6703 HD, The Netherlands.
authors.forename: Guido J
authors.initials: GJ
authors.lastname: Hooiveld
authors.affiliation: Key Laboratory of Human Functional Genomics Jiangsu Province, Nanjing Medical University , Nanjing 210029, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiang Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiang Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiang Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiang Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Xinglian
authors.initials: X
authors.lastname: Xu
authors.affiliation: Norwich Medical School, University of East Anglia , Norwich NR4 7TJ, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: Muller
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiang Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Chunbao
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Animal Products Processing, MOA; Jiang Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University , Nanjing 210095, P.R. China.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000378:metabolism; D002645:Chickens; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism*; D005399:Fishes; D005526:Food, Formulated; D005786:Gene Expression Regulation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008461:Meat Products / Q000032:analysis*; D053858:Metabolic Networks and Pathways / Q000187:drug effects* / Q000235:genetics; D008894:Milk Proteins / Q000008:administration & dosage / Q000378:metabolism; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D030262:Soybean Proteins / Q000008:administration & dosage / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D008894:Milk Proteins; D047493:PPAR alpha; D047495:PPAR gamma; D020543:Proteome; D030262:Soybean Proteins; D051059:PTEN Phosphohydrolase; C487216:Pten protein, rat
keywords: animal protein; chicken protein; fish protein; isolated protein; metabolic syndrome; molecular nutrition; nutrigenomics; pork protein; proteomics