title: Comparative Analysis of Human Mesenchymal Stem Cells from Umbilical Cord, Dental Pulp, and Menstrual Blood as Sources for Cell Therapy.
abstract: Although mesenchymal stem cells (MSCs) based therapy has been considered as a promising tool for tissue repair and regeneration, the optimal cell source remains unknown. Umbilical cord (UC), dental pulp (DP), and menstrual blood (MB) are easily accessible sources, which make them attractive candidates for MSCs. The goal of this study was to compare the biological characteristics, including morphology, proliferation, antiapoptosis, multilineage differentiation capacity, and immunophenotype of UC-, DP-, and MB-MSCs in order to provide a theoretical basis for clinical selection and application of these cells. As a result, all UC-, DP-, and MB-MSCs have self-renewal capacity and multipotentiality. However, the UC-MSCs seemed to have higher cell proliferation ability, while DP-MSCs may have significant advantages for osteogenic differentiation, lower cell apoptosis, and senescence. These differences may be associated with the different expression level of cytokines, including vascular endothelial growth factor, fibroblast growth factor, keratinocyte growth factor, and hepatocyte growth factor in each of the MSCs. Comprehensively, our results suggest DP-MSCs may be a desired source for clinical applications of cell therapy.
authors.affiliation: Cell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, China.
authors.forename: Huaijuan
authors.initials: H
authors.lastname: Ren
authors.affiliation: Cell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Sang
authors.affiliation: Cell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Asia Stem Cell BK, Limited, Shanghai 201203, China.
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Cell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, China.
authors.forename: Nianmin
authors.initials: N
authors.lastname: Qi
authors.affiliation: Cell Culture and Bioprocess Engineering Lab, School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, Minhang District, Shanghai 200240, China.
authors.forename: Yantian
authors.initials: Y
authors.lastname: Chen