title: Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros).
abstract: RNA interference (RNAi) is a gene silencing mechanism that is present in animals and plants and is triggered by double stranded RNA (dsRNA) or small interfering RNA (siRNA), depending on the organism. In the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), RNAi can be achieved by feeding rootworms dsRNA added to artificial diet or plant tissues transformed to express dsRNA. The effect of RNAi depends on the targeted gene function and can range from an absence of phenotypic response to readily apparent responses, including lethality. Furthermore, RNAi can directly affect individuals that consume dsRNA or the effect may be transferred to the next generation. Our previous work described the potential use of genes involved in embryonic development as a parental RNAi technology for the control of WCR. In this study, we describe the use of chromatin-remodeling ATPases as target genes to achieve parental gene silencing in two insect pests, a coleopteran, WCR, and a hemipteran, the Neotropical brown stink bug, Euschistus heros Fabricius (Hemiptera: Pentatomidae). Our results show that dsRNA targeting chromatin-remodeling ATPase transcripts, brahma, mi-2, and iswi strongly reduced the fecundity of the exposed females in both insect species. Additionally, knockdown of chd1 reduced the fecundity of E. heros.
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Elane
authors.initials: E
authors.lastname: Fishilevich
authors.affiliation: University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Vélez
authors.affiliation: University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.
authors.forename: Chitvan
authors.initials: C
authors.lastname: Khajuria
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Meghan L F
authors.initials: ML
authors.lastname: Frey
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Ronda L
authors.initials: RL
authors.lastname: Hamm
authors.affiliation: University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.
authors.forename: Haichuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Greg A
authors.initials: GA
authors.lastname: Schulenberg
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Bowling
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Heather E
authors.initials: HE
authors.lastname: Pence
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Premchand
authors.initials: P
authors.lastname: Gandra
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Kanika
authors.initials: K
authors.lastname: Arora
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Storer
authors.affiliation: Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States.
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Narva
authors.affiliation: Entomology and Nematology Department, Charles Steinmetz Hall, University of Florida, P.O. Box 110620, Gainesville, FL 32611-0620, United States. Electronic address: bsiegfried1@ufl.edu.
authors.forename: Blair D
authors.initials: BD
authors.lastname: Siegfried
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D001517:Coleoptera / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D005260:Female; D005298:Fertility; D020062:Heteroptera / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D007300:Insect Control; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D010572:Pest Control, Biological; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D002843:Chromatin; D019476:Insect Proteins; D034741:RNA, Small Interfering; D000251:Adenosine Triphosphatases
keywords: Brahma; Chd1; Chromatin-remodeling ATPase; Diabrotica; Iswi; Mi-2; Parental RNAi; Stink bug