title: [Radiofrequency ablation can reverse the abnormal circulating microRNA expression changes in patients with atrial fibrillation].
abstract: OBJECTIVE
  To observe the change of circulating microRNAs(miRNAs), regulatory mechanism in patients with atrial fibrillation (AF) before and after radiofrequency ablation (RFA).
  
  
  METHODS
  From January 2011 to December 2013, peripheral blood samples were taken from 30 AF patients (10 paroxysmal, 10 persistent and 10 permanent AF) before and 3 months after RFA. The total RNA was extracted and hybridized with the miRNA chips, and the differential expression of miRNA and clustering analysis in whole genome were made with Volcano Plot and tMEV software respectively, and validated by real-time PCR. The target gene analysis of miRNAs was predicted through the Mirbase, Miranda and Targetscan databases. Results were compared with those from 10 healthy subjects (control group).
  
  
  RESULTS
  Compared with control group, the expressions of 25 miRNAs were down-regulated before RFA and up-regulated after RFA in AF group, while other 40 miRNAs expression changed in the opposite way; among them, the expressions of 7 miRNAs including miR-199a-3p/miR-199b-3p were down- regulated >1.5-fold before RFA and up-regulated>100-fold after RFA; oppositely, 6 miRNAs including miR-BART8-3p were up-regulated>1.5-fold before RFA and down-regulated>10-fold after RFA. Interestingly, 6 miRNAs including miR-30b-5p, which were involved in AF-related electrical and structural remodeling, were down-regulated>5-fold before RFA, but up-regulated>50-fold after RFA. Four miRNAs including miR-377-5p, which were involved in the regulation of CACNA1C ICaL channel protein, were different before and after RFA.
  
  
  CONCLUSIONS
  miRNAs regulate the occurrence and development of AF. RFA can change the expression of miRNAs in AF patients, which may be important for reversing the electrical and structural remodeling and maintaining sinus rhythm after RFA. miRNAs, such as miR-30b-5p, miR-377-5p and miR-199a-3p/miR-199b-3p etc., might become the target markers for early diagnosis and intervention of AF in future.
authors.affiliation: Department of Cardiology, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Cardiology, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Cardiology, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China.
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Cardiology, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Nan
authors.affiliation: Department of Cardiology, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China.
authors.forename: Yunan
authors.initials: Y
authors.lastname: Yue
authors.affiliation: Department of Cardiology, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing 100038, China; Email: sxyang68@163.com.
authors.forename: Shuixiang
authors.initials: S
authors.lastname: Yang
mesh_terms: D001281:Atrial Fibrillation; D017115:Catheter Ablation; D016000:Cluster Analysis; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs