title: Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway.
abstract: The activity of a local renin-angiotensin system (RAS) in the adipose tissue is closely associated with obesity-related diseases. However, the mechanism of RAS activation in adipose tissue is still unknown. In the current study, we found that palmitic acid (PA), one kind of free fatty acid, induced the activity of RAS in 3T3-L1 adipocytes. In the presence of fetuin A (Fet A), PA upregulated the expression of angiotensinogen (AGT) and angiotensin type 1 receptor (AT1R) and stimulated the secretion of angiotensin II (ANG II) in 3T3-L1 adipocytes. Moreover, the activation of RAS in 3T3-L1 adipocytes was blocked when we blocked Toll-like receptor 4 (TLR4) signaling pathway using TAK242 or NF-κB signaling pathway using BAY117082. Together, our results have identified critical molecular mechanisms linking PA/TLR4/NF-κB signaling pathway to the activity of the local renin-angiotensin system in adipose tissue.
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Geratology, The Affiliated Hospital of Guangdong Medical College, Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: Department of Epidemiology and Medical Statistics, School of Public Health, Guangdong Medical College, Dongguan, Guangdong, China.
authors.forename: Liangchang
authors.initials: L
authors.lastname: Xiu
authors.affiliation: Second Clinical School of Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Bimei
authors.initials: B
authors.lastname: Fang
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Nephrology Center of Integrated Traditional Chinese and Western Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects*; D000804:Angiotensin II / Q000378:metabolism; D000808:Angiotensinogen / Q000032:analysis; D000818:Animals; D051379:Mice; D016328:NF-kappa B / Q000502:physiology*; D019308:Palmitic Acid / Q000494:pharmacology*; D044140:Receptor, Angiotensin, Type 1 / Q000032:analysis; D012084:Renin-Angiotensin System / Q000187:drug effects* / Q000502:physiology; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000502:physiology; D060749:alpha-2-HS-Glycoprotein / Q000494:pharmacology
chemical_list: D016328:NF-kappa B; D044140:Receptor, Angiotensin, Type 1; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D060749:alpha-2-HS-Glycoprotein; D000808:Angiotensinogen; D000804:Angiotensin II; D019308:Palmitic Acid