title: Safflower extract inhibiting apoptosis by inducing autophagy in myocardium derived H9C2 cell.
abstract: The Heart failure (HF) is considered as the end-stage of various heart disease and associated with high mortality globally. Progressive loss of cardiac myocytes via apoptosis is considered as the most important factor for HF pathology. In this study, we demonstrated that Safflower extract was able to inhibitthe apoptosis inducted by Angiotensin II (AngII) in a ratmyocardium derived cell line H9C2. Further examination of LC-3II conversion and autophagosome formation suggested Safflower extract induced autophagy in treated cell. Inhibition of Safflower extract induced autophagy by 3-methyladenine (3MA) abolished anti-apoptotic function of Safflower extract, while application of autophagy stimulator Rapamycin in H9C2 inhibited apoptosis as well. Moreover, treatment of H9C2 cell with Safflower extract also inhibited expression of pro-apoptotic genes BAD and Bax. In conclusion, our data indicated that Safflower extract inhibit apoptosis via inducing autophagy in myocardium cell and demonstrated the potential as novel therapeutic drug for Heart failure.
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Yancai
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Huailing
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Tenjiao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Youbo
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Jinan City Jinan 250022, Shandong, China.
authors.forename: Shimin
authors.initials: S
authors.lastname: Liang
keywords: Heart failure; apoptosis; autophagy; pro-apoptotic genes; safflower extract