title: Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats.
abstract: Background. The incidence of metabolic syndrome co-existing with diabetes mellitus is on the rise globally. Objective. The present study was designed to develop a unique animal model that will mimic the pathological features seen in individuals with diabetes and metabolic syndrome, suitable for pharmacological screening of drugs. Materials and Methods. A combination of High-Fat Diet (HFD) and low dose of streptozotocin (STZ) at 30, 35, and 40 mg/kg was used to induce metabolic syndrome in the setting of diabetes mellitus in Wistar rats. Results. The 40 mg/kg STZ produced sustained hyperglycemia and the dose was thus selected for the study to induce diabetes mellitus. Various components of metabolic syndrome such as dyslipidemia {(increased triglyceride, total cholesterol, LDL cholesterol, and decreased HDL cholesterol)}, diabetes mellitus (blood glucose, HbA1c, serum insulin, and C-peptide), and hypertension {systolic blood pressure} were mimicked in the developed model of metabolic syndrome co-existing with diabetes mellitus. In addition to significant cardiac injury, atherogenic index, inflammation (hs-CRP), decline in hepatic and renal function were observed in the HF-DC group when compared to NC group rats. The histopathological assessment confirmed presence of edema, necrosis, and inflammation in heart, pancreas, liver, and kidney of HF-DC group as compared to NC. Conclusion. The present study has developed a unique rodent model of metabolic syndrome, with diabetes as an essential component.
authors.affiliation: Department of Pharmacology, MGM Medical College, Kamothe, Navi Mumbai 410209, India.
authors.forename: Rajesh Kumar
authors.initials: RK
authors.lastname: Suman
authors.affiliation: Department of Pharmacology, MGM Medical College, Kamothe, Navi Mumbai 410209, India.
authors.forename: Ipseeta
authors.initials: I
authors.lastname: Ray Mohanty
authors.affiliation: Department of Pharmacology, MGM Medical College, Kamothe, Navi Mumbai 410209, India.
authors.forename: Manjusha K
authors.initials: MK
authors.lastname: Borde
authors.affiliation: Department of Pathology, MGM Medical College, Kamothe, Navi Mumbai 410209, India.
authors.forename: Ujwala
authors.initials: U
authors.lastname: Maheshwari
authors.affiliation: Department of Pharmacology, MGM Medical College, Kamothe, Navi Mumbai 410209, India.
authors.forename: Y A
authors.initials: YA
authors.lastname: Deshmukh