title: Treatment of colon cancer with oncolytic herpes simplex virus in preclinical models.
abstract: Cancer stem cells (CSCs), which are a rare population in any type of cancer, including colon cancer, are tumorigenic and responsible for cancer recurrence and metastasis. CSCs have been isolated from a number of different solid tumors recently, although the isolation of CSCs in colon cancer is still challenging. We cultured colon cancer cells in stem cell medium to obtain colonosphere cells. These cells possessed the characteristics of CSCs, with a high capacity of tumorigenicity, migration and invasion in vitro and in vivo. The isolation and identification of CSCs have provided new targets for the therapeutics. Oncolytic herpes simplex viruses (oHSV) are an effective strategy for killing colon cancer cells in preclinical models. Here, we examined the efficacy of an oncolytic herpes simplex virus type 2 (oHSV2) in killing colon cancer cells and colon cancer stem-like cells (CSLCs). oHSV2 was found to be highly cytotoxic to the adherent and sphere cells in vitro, and oHSV2 treatment in vivo significantly inhibited tumor growth. This study demonstrates that oHSV2 is effective against colon cancer cells and colon CSLCs and could be a promising strategy for treating colon cancer patients.
authors.affiliation: Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.|Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuchang, Wuhan, China.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Blood Transfusion, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Plastic and Aesthetic Surgery, The Third Hospital of Wuhan City, Wuhan, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuchang, Wuhan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Geriatric Medicine, Puai Hospital of Wuhan City, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: S
authors.initials: S
authors.lastname: Peng
authors.affiliation: Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: T
authors.initials: T
authors.lastname: Du
authors.affiliation: School of Pharmacology, Hubei University of Science and Technology, Xianning, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuchang, Wuhan, China.|School of Pharmacology, Hubei University of Science and Technology, Xianning, China.
authors.forename: B
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics / Q000628:therapy* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000235:genetics*; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000473:pathology; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays