title: Enzymatic generation of peptides from potato proteins by selected proteases and characterization of their structural properties.
abstract: The use of low grade starting material for the generation of peptides with bioactivity properties is of interest. The proteins from the potato starch industry byproduct is a promising source, as several health benefits may be associated with their hydrolysates. The efficiency of selected proteases (Novo Pro-D, Alcalase, Flavourzyme, and Papain), exhibiting different substrate specificities and cleavage action modes, to hydrolyze potato protein isolate (patatin and protease inhibitors) was investigated. Novo Pro-D resulted in the lowest degree of hydrolysis, whereas Alcalase, Flavourzyme, and Papain exhibited a high catalytic efficiency for the hydrolysis of potato proteins. The degree of hydrolysis behaved in a concentration dependent manner. However, the end-product profile (peptides and free amino acids) was dependent not only on the protease specificity, its cleavage action mode (endo/exo) and the availability of the intermediate substrates but also on the contribution of the protease inhibitors to the reaction kinetics through their inhibitory effects. Indeed, the dependence of the exo-activity on the catalytic efficiency of the endo-action of protease was shown to be significant. Papain generated more unique peptide sequences with homology assessment matching several potato proteins when compared with Flavourzyme. This can be attributed to the high exo-peptidase activity of Flavourzyme resulting in the generation of shorter peptides which were difficult to match. Flavourzyme produced more peptides originated from patatin fraction, whereas Papain resulted in the release of more peptides corresponding to the protease inhibitor fractions. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:420-429, 2016.
authors.affiliation: Dept. of Food Science and Agricultural Chemistry, McGill University, 21 111 Lakeshore, Ste. Anne De Bellevue, Quebec, H9X 3V9, Canada.
authors.forename: Amanda
authors.initials: A
authors.lastname: Waglay
authors.affiliation: Dept. of Food Science and Agricultural Chemistry, McGill University, 21 111 Lakeshore, Ste. Anne De Bellevue, Quebec, H9X 3V9, Canada.
authors.forename: Salwa
authors.initials: S
authors.lastname: Karboune
mesh_terms: D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011487:Protein Conformation; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D010455:Peptides; D010940:Plant Proteins; D010447:Peptide Hydrolases
keywords: Flavourzyme; Papain; enzymatic hydrolysis; peptides; potato protein isolates