title: Simple and versatile synthetic polydopamine-based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions.
abstract: Human pluripotent stem cells (hPSCs) possess great value in the aspect of cellular therapies due to its self-renewal and potential to differentiate into all somatic cell types. A few defined synthetic surfaces such as polymers and adhesive biological materials conjugated substrata were established for the self-renewal of hPSCs. However, none of them was effective in the generation of human induced pluripotent stem cells (hiPSCs) and long-term maintenance of multiple hPSCs, and most of them required complicated manufacturing processes. Polydopamine has good biocompatibility, is able to form a stable film on nearly all solid substrates surface, and can immobilize adhesive biomolecules. In this manuscript, a polydopamine-mediated surface was developed, which not only supported the reprogramming of human somatic cells into hiPSCs under defined conditions, but also sustained the growth of hiPSCs on diverse substrates. Moreover, the proliferation and pluripotency of hPSCs cultured on the surface were comparable to Matrigel for more than 20 passages. Besides, hPSCs were able to differentiate to cardiomyocytes and neural cells on the surface. This polydopamine-based synthetic surface represents a chemically-defined surface extensively applicable both for fundamental research and cell therapies of hPSCs.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing, 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.
authors.forename: Fujian
authors.initials: F
authors.lastname: Wu
authors.affiliation: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
authors.forename: Tiancheng
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Cai
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China; Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing, 100081, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing, 100081, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing, 100081, China.
authors.forename: Mengke
authors.initials: M
authors.lastname: Wang
authors.affiliation: Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lan
authors.affiliation: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. Electronic address: pan_guangjin@gibh.ac.cn.
authors.forename: Guangjin
authors.initials: G
authors.lastname: Pan
authors.affiliation: Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
authors.forename: Duanqing
authors.initials: D
authors.lastname: Pei
authors.affiliation: Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing, 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China. Electronic address: sc-wei@pku.edu.cn.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D065150:Cellular Reprogramming; D048271:Chitosan / Q000031:analogs & derivatives; D000066449:Human Embryonic Stem Cells / Q000166:cytology; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D007211:Indoles / Q000737:chemistry*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D019096:Vitronectin / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D055767:Immobilized Proteins; D007211:Indoles; D010455:Peptides; D011108:Polymers; D019096:Vitronectin; C568283:polydopamine; D048271:Chitosan
keywords: Carboxymethyl chitosan; Chemically-defined; Human embryonic stem cells; Human induced pluripotent stem cells; Peptide; Polydopmine