title: Control of sulfide and methane production in anaerobic sewer systems by means of Downstream Nitrite Dosage.
abstract: Bioproduction of hydrogen sulfide (H2S) and methane (CH4) under anaerobic conditions in sewer pipes causes detrimental effects on both sewer facilities and surrounding environment. Among the strategies used to mitigate the production of both compounds, the addition of nitrite (NO2(-)) has shown a greater long-term inhibitory effect compared with other oxidants such as nitrate or oxygen. The aim of this study was to determine the effectiveness of a new method, the Downstream Nitrite Dosage strategy (DNO2D), to control H2S and CH4 emissions in sewers. Treatment effectiveness was assessed on H2S and CH4 abatement on the effluent of a laboratory sewer pilot plant that mimics a full-scale anaerobic rising sewer. The experiment was divided in three different periods: system setup (period 1), nitrite addition (period 2) and system recovery (period 3). Different process and molecular methods were combined to investigate the impact of NO2(-) addition on H2S and CH4 production. Results showed that H2S load was reduced completely during nitrite addition when compared to period 1 due to H2S oxidation but increased immediately after nitrite addition stopped. The H2S overproduction during recovery period was associated with the bacterial reduction of different sulfur species (elemental sulfur/thiosulfate/sulfite) accumulated within the sewer biofilm matrix. Oxidation of CH4 was also detected during period 2 but, contrary to sulfide production, re-establishment of methanogenesis was not immediate after stopping nitrite dosing. The analysis of bulk and active microbial communities along experimental treatment showed compositional changes that agreed with the observed dynamics of chemical processes. Results of this study show that DNO2D strategy could significantly reduce H2S and CH4 emissions from sewers during the addition period but also suggest that microbial agents involved in such processes show a high resilience towards chemical stressors, thus favoring the re-establishment of H2S and CH4 production after stopping nitrite addition.
authors.affiliation: Catalan Institute for Water Research (ICRA), Scientific and Technological Park UdG, Girona, Spain. Electronic address: oauguet@icra.cat.
authors.forename: Olga
authors.initials: O
authors.lastname: Auguet
authors.affiliation: Catalan Institute for Water Research (ICRA), Scientific and Technological Park UdG, Girona, Spain. Electronic address: mpijuan@icra.cat.
authors.forename: Maite
authors.initials: M
authors.lastname: Pijuan
authors.affiliation: Catalan Institute for Water Research (ICRA), Scientific and Technological Park UdG, Girona, Spain; Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of Girona, Girona, Spain. Electronic address: cborrego@icra.cat.
authors.forename: Carles M
authors.initials: CM
authors.lastname: Borrego
authors.affiliation: Catalan Institute for Water Research (ICRA), Scientific and Technological Park UdG, Girona, Spain. Electronic address: ogutierrez@icra.cat.
authors.forename: Oriol
authors.initials: O
authors.lastname: Gutierrez
keywords: Methane; Methanogenic archaea; Nitrite; Sewer biofilms; Sulfate-reducing bacteria; Sulfide