title: miRNA-101 inhibits ovarian cancer cells proliferation and invasion by down-regulating expression of SOCS-2.
abstract: OBJECTIVE
  To investigate the expression of miRNA-101 in normal and malignant ovarian tissues and cells as well as its impact on the proliferation and invasion of human ovarian cancer H08910 and SKOV3 cell lines.
  
  
  METHODS
  Real time polymerase chain reaction (RT-PCR) was employed to detect the miR-101 and SOCS-2 expression in 20 separate ovarian cancer tissues and para-carcinoma tissues, human ovarian cancer cells (H08910 and SKOV3) and normal human ovarian epithelial cells (HUM-CELL-0088). After H08910 and SKOV3 ovarian cancer cells were respectively transfected with miR-NC (H08910/NC and SKOV3/NC) and miR-101 (H08910/miR-101 and SKOV3/miR-101), Western Blot was employed to detect the SOCS-2 expression in transfected cells. CCK-8 and clone formation and Transwell assays were employed to determine the proliferation and invasion ability of wild type and transfected ovarian cancer cells.
  
  
  RESULTS
  The expression of miR-101 in ovarian cancer tissues and cells was significantly lower than that in para-carcinoma tissues (t=19.12, P=0.002) and normal human ovarian epithelial cells (HUM-CELL-0088) (F=14.37, P=0.000), respectively. In contrast, the SOCS-2 expression in ovarian cancer tissues and cells was significantly higher than that in para-carcinoma tissues (t=25.03, P=0.000) and HUM-CELL-0088 cells (F=14.9, P=0.000) by Western Blotting analysis, respectively. Compared with wild type and empty vector transfected cells, the expression of SOCS-2 was significantly decreased in miR-101 transfected H08910 (t=10.9, P=0.001) and SKOV3 cells (t=21.03, P=0.000). The results of CCK-8, clone formation and Transwell assays revealed that the proliferation and invasion ability of ovarian cancer cells was markedly inhibited by the transfection of miR-101.
  
  
  CONCLUSIONS
  MiR-101 was validated to be reduced and SOCS-2 expression increased in ovarian cancer tissues and cells. The over expression of miR-101 can remarkably reduce the in vitro proliferation and invasion ability of ovarian cancer cells through the down-regulation of SOCS-2.
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Zheng
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Zheng
authors.affiliation: Department of Nuclear Medicine, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, China.
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Liu
keywords: SOCS-2; cell proliferation; miRNA-101; ovarian cancer