title: Species clarification of Isaria isolates used as biocontrol agents against Diaphorina citri (Hemiptera: Liviidae) in Mexico.
abstract: Entomopathogenic fungi belonging to the genus Isaria (Hypocreales: Cordycipitaceae) are promising candidates for microbial control of insect pests. Currently, the Mexican government is developing a biological control program based on extensive application of Isaria isolates against Diaphorina citri (Hemiptera: Liviidae), a vector of citrus huanglongbing disease. Previous research identified three promising Isaria isolates (CHE-CNRCB 303, 305, and 307; tentatively identified as Isaria fumosorosea) from Mexico. The goal of this work was to obtain a complete morphological and molecular characterization of these isolates. Comparative analysis of morphology established that the isolates showed similar characteristics to Isaria javanica. Multi-gene analysis confirmed the morphological identification by including the three isolates within the I. javanica clade. Additionally, this work demonstrated the misidentifications of three other Isaria isolates (CHE-CNRCB 310 and 324: I. javanica, formerly I. fumosorosea; CHE-CNRCB 393: I. fumosorosea, formerly Isaria farinosa), underlying the need for a full and correct characterization of an isolate before developing a biological control program. Finally, the inter-simple sequence repeat (ISSR) genotyping method revealed that the CHE-CNRCB 303, 305, and 307 isolates belong to three different genotypes. This result indicates that ISSR markers could be used as a tool to monitor their presence in field conditions.
authors.affiliation: Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico. Electronic address: gallou.adrien@hotmail.fr.
authors.forename: Adrien
authors.initials: A
authors.lastname: Gallou
authors.affiliation: Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
authors.forename: María G
authors.initials: MG
authors.lastname: Serna-Domínguez
authors.affiliation: Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
authors.forename: Angélica M
authors.initials: AM
authors.lastname: Berlanga-Padilla
authors.affiliation: Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Ayala-Zermeño
authors.affiliation: Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Mellín-Rosas
authors.affiliation: Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Montesinos-Matías
authors.affiliation: Centro Nacional de Referencia de Control Biológico, Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac, C.P. 28110, Tecomán, Colima, Mexico.
authors.forename: Hugo C
authors.initials: HC
authors.lastname: Arredondo-Bernal
mesh_terms: D000818:Animals; D002957:Citrus / Q000469:parasitology; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006430:Hemiptera / Q000382:microbiology*; D006999:Hypocreales / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D008800:Mexico; D008853:Microscopy; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010572:Pest Control, Biological; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000469:parasitology / Q000517:prevention & control; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D014404:Tubulin
keywords: Biological control; Entomopathogenic fungi; ISSR markers; Isaria javanica; Morphology; Phylogeny