title: Role of BCL2-associated athanogene in resistance to platinum-based chemotherapy in non-small-cell lung cancer.
abstract: The present study aimed to address the pharmacogenetic role of BAG1 in platinum-based chemotherapy in advanced non-small-cell lung cancer (NSCLC) and in cultured human lung adenocarcinoma A549 cells. A total of 108 NSCLC patients (stages I-IIIA) were treated with a standard chemotherapy regimen of cisplatin plus vinorelbine. Additionally,in vitrocultured A549 cells were treated with cisplatin in the presence or absence of tunicamycin. Cell proliferation was determined by MTT assay and protein levels were assessed via western blot analysis. Patients with BAG1-positive expression were revealed to have a prolonged survival time (progression-free survival, 24.0 months) compared with that of patients without BAG1 expression (21.6 months; χ2=18.018, P<0.05). Treatment of A549 cells with tunicamycin followed by cisplatin resulted in elevated BAG1 levels. In addition, tunicamycin was found to significantly enhance cisplatin-induced growth inhibition and apoptosis in A549 cells. The results indicate that BAG1 is important in cisplatin-induced cell death in lung adenocarcinoma, suggesting that endoplasmic reticulum stress may promote the sensitivity of NSCLC patients to chemotherapy.
authors.affiliation: Department of Oncology, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Yadi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology, First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Minwen
authors.initials: M
authors.lastname: Ha
authors.affiliation: Department of Heart Surgery, First Affiliated Hospital of Xuzhou Medical College, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Jingsong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Oncology, First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Oncology, First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
keywords: BCL2-associated athanogene; chemotherapy; cisplatin; non-small cell lung cancer