title: Noise analysis for through-focus scanning optical microscopy.
abstract: A systematic noise-analysis study for optimizing data collection and data processing parameters for through-focus scanning optical microscopy (TSOM) is presented. TSOM is a three-dimensional shape metrology method that can achieve sub-nanometer measurement sensitivity by analyzing sets of images acquired through focus using a conventional optical microscope. We show that the best balance between signal-to-noise performance and acquisition time can be achieved by judicious spatial averaging. Correct background-signal subtraction of the imaging system inhomogeneities is also critical, as well as careful alignment of the constituent images in the case of differential TSOM analysis.
authors.forename: Ravikiran
authors.initials: R
authors.lastname: Attota
mesh_terms: D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena; D059629:Signal-To-Noise Ratio