title: Near-infrared light (670 nm) reduces MPTP-induced parkinsonism within a broad therapeutic time window.
abstract: We have shown previously that near-infrared light (NIr), when applied at the same time as a parkinsonian insult (e.g. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; MPTP), reduces behavioural deficits and offers neuroprotection. Here, we explored whether the timing of NIr intervention-either before, at the same time or after the MPTP insult-was important. Mice received MPTP injections (total of 50 mg/kg) and, at various stages in relation to these injections, extracranial application of NIr. Locomotor activity was tested with an open-field test, and brains were processed for immunohistochemistry. Our results showed that regardless of when NIr was applied in relation to MPTP insult, behavioural impairment was reduced by a similar magnitude. The beneficial effect of NIr was fast-acting (within minutes) and long-lasting (for several days). There were more dopaminergic cells in the NIr-treated MPTP groups than in the MPTP group; there was no clear indication that a particular combination of NIr treatment and MPTP injection resulted in a higher cell number. In summary, irrespective of whether it was applied before, at the same time as or after MPTP insult, NIr reduced both behavioural and structural measures of damage by a similar magnitude. There was a broad therapeutic time window of NIr application in relation to the stage of toxic insult, and the NIr was fast-acting and long-lasting.
authors.affiliation: CLINATEC, EJ Safra Centre, CEA, LETI, University of Grenoble Alpes, 38000, Grenoble, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Reinhart
authors.affiliation: Department of Anatomy F13, University of Sydney, Sydney, 2006, Australia.
authors.forename: Nabil
authors.initials: N
authors.lastname: El Massri
authors.affiliation: Department of Physiology F13, University of Sydney, Sydney, 2006, Australia.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Johnstone
authors.affiliation: Department of Physiology F13, University of Sydney, Sydney, 2006, Australia.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Stone
authors.affiliation: Department of Anatomy F13, University of Sydney, Sydney, 2006, Australia. john.mitrofanis@sydney.edu.au.
authors.forename: John
authors.initials: J
authors.lastname: Mitrofanis
authors.affiliation: CLINATEC, EJ Safra Centre, CEA, LETI, University of Grenoble Alpes, 38000, Grenoble, France.
authors.forename: Alim-Louis
authors.initials: AL
authors.lastname: Benabid
authors.affiliation: CLINATEC, EJ Safra Centre, CEA, LETI, University of Grenoble Alpes, 38000, Grenoble, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Moro
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000528:radiation effects*; D004195:Disease Models, Animal; D007259:Infrared Rays / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D020267:MPTP Poisoning / Q000517:prevention & control / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000528:radiation effects*; D010789:Phototherapy / Q000379:methods*; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors
keywords: Neuroprotection; Open-field test; Parkinson’s disease; Photobiomodulation; Substantia nigra