title: Combining cross-crystal averaging and MRSAD to phase a 4354-amino-acid structure.
abstract: The B and C proteins from the ABC toxin complex of Yersinia entomophaga form a large heterodimer that cleaves and encapsulates the C-terminal toxin domain of the C protein. Determining the structure of the complex formed by B and the N-terminal region of C was challenging owing to its large size, the non-isomorphism of different crystals and their sensitivity to radiation damage. A native data set was collected to 2.5 Å resolution and a non-isomorphous Ta6Br12-derivative data set was collected that showed strong anomalous signal at low resolution. The tantalum-cluster sites could be found, but the anomalous signal did not extend to a high enough resolution to allow model building. Selenomethionine (SeMet)-derivatized protein crystals were produced, but the high number (60) of SeMet sites and the sensitivity of the crystals to radiation damage made phasing using the SAD or MAD methods difficult. Multiple SeMet data sets were combined to provide 30-fold multiplicity, and the low-resolution phase information from the Ta6Br12 data set was transferred to this combined data set by cross-crystal averaging. This allowed the Se atoms to be located in an anomalous difference Fourier map; they were then used in Auto-Rickshaw for multiple rounds of autobuilding and MRSAD.
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
authors.forename: Jason Nicholas
authors.initials: JN
authors.lastname: Busby
authors.affiliation: School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
authors.forename: J Shaun
authors.initials: JS
authors.lastname: Lott
authors.affiliation: MX, Australian Synchrotron, 800 Blackburn Road, Clayton, Melbourne, VIC 3168, Australia.
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D018360:Crystallography, X-Ray / Q000379:methods; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry; D020836:Protein Structure, Quaternary; D015007:Yersinia
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D046912:Multiprotein Complexes
keywords: Auto-Rickshaw; MRSAD; cross-crystal averaging; phasing