title: Regulation of proliferation, angiogenesis and apoptosis in hepatocellular carcinoma by miR-26b-5p.
abstract: MicroRNAs (miRNAs) play vital roles in cell proliferation, differentiation and apoptosis in hepatocellular carcinoma (HCC). miR-26b has been confirmed as an important regulator in carcinogenesis and other pathological processes. miR-26b-5p is one member of the mature miR-26 family, and its functional role in proliferation, angiogenesis and apoptosis in HCC remains unknown. Here, we demonstrate that miR-26b-5p expression was significantly decreased in HCC tissues and HCC cell lines compared with normal liver tissues and liver cells by quantitative real-time polymerase chain reaction (qRT-PCR). The relationships between miR-26b-5p and the clinical characteristics of HCC patients were further analysed, and miR-26b-5p was positively correlated with the differentiation of HCC cells. Computational searches were further used to identify the downstream targets and signalling pathways of miR-26b-5p in HCC cells. Cell viability, proliferation and tube formation abilities were assessed by scrape, 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and three-dimensional culture assays to confirm that miR-26b-5p inhibited HCC cell growth and impaired the tube formation ability of the HCC cells. Both in vitro and in vivo studies showed that miR-26b-5p could suppress vascular mimicry (VM) and angiogenesis by down-regulating the expression of VE-cadherin, Snail and MMP2 and could inhibit the apoptosis of HCC cells. Using mouse models, we revealed that tumours derived from miR-26b-5p-expressing HCC cells displayed a significant decrease in microvessel density compared with those derived from control cells. Therefore, our data provide further insight into the role of miR-26b-5p as a negative regulator of proliferation, angiogenesis, and apoptosis in HCC.
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China. baocunsun@aliyun.com.|Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, 300060, China. baocunsun@aliyun.com.|Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052, China. baocunsun@aliyun.com.|Department of Pathology and Cancer Hospital and General Hospital of Tianjin, Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin, 300070, People's Republic of China. baocunsun@aliyun.com.
authors.forename: Baocun
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Huizhi
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.|Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052, China.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, 300060, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin, 300060, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.|Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.|Department of Pathology, Tianjin General Hospital, Tianjin Medical University, Tianjin, 300052, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Pathology, Tianjin Medical University, Tianjin, 300070, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Shao
authors.affiliation: Department of Pathology, Mudanjiang Medical University, Heilongjiang, 157011, China.
authors.forename: Jindan
authors.initials: J
authors.lastname: An
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; C528163:MIRN26 microRNA, human
keywords: Angiogenesis; Apoptosis; Hepatocellular carcinoma; miR-26b-5p