title: MicroRNA profile analysis of host cells before and after wild human rotavirus infection.
abstract: Rotavirus infection is an important cause of acute gastroenteritis in children, but the interaction between rotavirus and host cells is not completely understood. We isolated a wildtype (wt) rotavirus strain, ZTR-68(P [8] G1), which is derived from an infant with diarrhea in southwest China in 2010. In this study, we investigated host cellular miRNA expression profiles changes in response to ZTR-68 in early stage of infection to investigate the role of miRNAs upon rotavirus infection. Differentially expressed miRNAs were identified by deep sequencing and qRT-PCR and the function of their targets predicted by Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. A total of 36 candidate miRNAs were identified. Comparative analysis indicated that 29 miRNAs were significantly down-regulated and 7 were up-regulated after infection. The data were provided contrasting the types of microRNAs in two different permissive cell lines (HT29 and MA104). The target assays results showed that mml-miR-7 and mml-miR-125a are involved in anti-rotavirus and virus-host interaction in host cells. These results offer clues for identifying potential candidates in vector-based antiviral strategies. J. Med. Virol. 88:1497-1510, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Jinyuan
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Geng
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Kui
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yin
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yi
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Kunming, China.
authors.forename: Maosheng
authors.initials: M
authors.lastname: Sun
mesh_terms: D015415:Biomarkers; D002460:Cell Line; D002681:China; D019295:Computational Biology; D003967:Diarrhea / Q000821:virology; D015536:Down-Regulation; D020869:Gene Expression Profiling; D019073:HT29 Cells; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification / Q000502:physiology*; D012400:Rotavirus Infections / Q000235:genetics* / Q000821:virology; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: deep sequence; host cells; microRNAs profile; rotavirus