title: Fascin 1 promoted the growth and migration of non-small cell lung cancer cells by activating YAP/TEAD signaling.
abstract: Fascin 1 (Fascin actin-bundling protein 1) is an actin-binding protein. Although several studies have reported the dysregulation of Fascin 1 in non-small cell lung cancer (NSCLC), its functions in the progression of NSCLC and the related molecular mechanism were not fully understood. In this study, the expression of Fascin 1 in NSCLC tissues was determined using quantitative PCR (qPCR), and the roles of Fascin 1 in the progression of NSCLC were investigated. It was found that both the messenger RNA (mRNA) level and the protein level of Fascin 1 were upregulated in NSCLC tissues. Forced expression of Fascin 1 promoted the growth and migration of NSCLC cells, while knocking down the expression of Fascin 1 inhibited the growth, migration, and tumorigenesis of NSCLC cells. Mechanism studies showed that Fascin 1 increased the transcriptional activity of the YAP/TEAD (Yes-associated protein/TEA domain transcriptional factor) complex, and knocking down the expression of Fascin 1 attenuated the expression of target genes downstream the YAP/TEAD complex. In addition, MST1 interacted with Fascin 1. Taken together, Fascin 1 plays an oncogenic role in NSCLC by activating the transcriptional activity of the YAP/TEAD complex.
authors.affiliation: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu, China.|Department of Thoracic Surgery, Ningbo First Hospital, Ningbo, Zhejiang, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu, China. uuzyshen@aliyun.com.
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Teng
authors.affiliation: Department of Thoracic Surgery, Ningbo First Hospital, Ningbo, Zhejiang, China.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Ningbo First Hospital, Ningbo, Zhejiang, China.
authors.forename: Chenwei
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Ningbo First Hospital, Ningbo, Zhejiang, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Thoracic Surgery, Ningbo First Hospital, Ningbo, Zhejiang, China.
authors.forename: Zenghui
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, Ningbo First Hospital, Ningbo, Zhejiang, China.
authors.forename: Zishan
authors.initials: Z
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008840:Microfilament Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000378:metabolism*; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C502658:FSCN1 protein, human; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D000091262:TEA Domain Transcription Factors; C057182:TEAD1 protein, human; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human
keywords: Cell growth; Fascin 1; Migration; NSCLC; YAP/TEAD signaling