title: Label-free electrochemical immunosensor based on Nile blue A-reduced graphene oxide nanocomposites for carcinoembryonic antigen detection.
abstract: In this article, a novel, label-free, and inherent electroactive redox immunosensor for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and Nile blue A (NB) hybridized electrochemically reduced graphene oxide (NB-ERGO) is proposed. The composite of NB-graphene oxide (NB-GO) was prepared by π-π stacking interaction. Then, chronoamperometry was adopted to simultaneously reduce HAuCl4 and nanocomposites of NB-GO for synthesizing AuNPs/NB-ERGO. The immunosensor was fabricated by capturing CEA antibody (anti-CEA) at this nanocomposite modified electrode. The immunosensor determination was based on the fact that, due to the formation of antigen-antibody immunocomplex, the decreased response currents of NB were directly proportional to the concentrations of CEA. Under optimal conditions, the linear range of the proposed immunosensor was estimated to be from 0.001 to 40 ng ml(-1) and the detection limit was estimated to be 0.00045 ng ml(-1). The proposed immunosensor was used to determine CEA in clinical serum samples with satisfactory results. The proposed method may provide promising potential application in clinical immunoassays with the properties of facile procedure, stability, high sensitivity, and selectivity.
authors.affiliation: College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China; Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China.
authors.forename: Yan-Sha
authors.initials: YS
authors.lastname: Gao
authors.affiliation: Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China. Electronic address: xujingkun@tsinghua.org.cn.
authors.forename: Jing-Kun
authors.initials: JK
authors.lastname: Xu
authors.affiliation: College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China. Electronic address: lulimin816@hotmail.com.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Lu
authors.affiliation: College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China.
authors.forename: Wen-Min
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China.
authors.forename: Tao-Tao
authors.initials: TT
authors.lastname: Yang
authors.affiliation: Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China.
authors.forename: Hua-Kun
authors.initials: HK
authors.lastname: Xing
authors.affiliation: College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China.
authors.forename: Yong-Fang
authors.initials: YF
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
chemical_list: D002272:Carcinoembryonic Antigen; D010078:Oxazines; D010087:Oxides; C008619:Nile Blue; D006108:Graphite
keywords: Au nanoparticles; Carcinoembryonic antigen; Label-free immunosensor; Redox probe; Reduced graphene oxide