title: [Xianglian External Lotion Restored the Sensitivity of Drug-resistant Candida albicans Strains to Fluconazole: a Transcriptomics Study].
abstract: OBJECTIVE
  To perform a transcriptomics study in differential genes after Xianglian External Lotion (XEL) induced the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole.
  
  
  METHODS
  Broth microdilution antifungal susceptibility test was used to detect minimal inhibitory concentration (MIC) of drug-resistant Candida albicans strains induced by XEL. Transcriptome sequencing (RNA-Seq) was used to determine and compare the transcription of primary drug-resistant Candida aIbicans strains and sensitive strains induced by XEL. High expressed genes and signaling pathways strains were analyzed by gene ontology (GO) method.
  
  
  RESULTS
  XEL could induce drug-resistant strains of the 6th generations to recover sensitivity. Transcriptome sequencing showed that, as compared with primary drug-resistant strains, there were 165 genes with up-regulated RPKM index and 144 genes with down-regulated RPKM index after XEL induction. GO analyses found that all genes were mainly classified as GO:0015903 (fluconazole transport).
  
  
  CONCLUSIONS
  XEL could induce the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole. By analyzing transcriptomes, authors speculated that XEL could recover strain sensitivity to fluconazole by opening fluconazole transport pathway.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Zhi-qi
authors.initials: ZQ
authors.lastname: Fan
authors.forename: Rui-qiang
authors.initials: RQ
authors.lastname: Fan
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002175:Candida; D002176:Candida albicans / Q000187:drug effects / Q000235:genetics; D002177:Candidiasis / Q000188:drug therapy; D025141:Drug Resistance, Fungal / Q000187:drug effects* / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D015725:Fluconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D004365:Drugs, Chinese Herbal; D015725:Fluconazole