title: Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation.
abstract: We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. Expanded CTCs are characterized at the genetic, protein and metabolic levels.
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China. qihuishi@sjtu.edu.cn.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China. qihuishi@sjtu.edu.cn.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wu
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China. qihuishi@sjtu.edu.cn.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China. qihuishi@sjtu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China. qihuishi@sjtu.edu.cn.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. tjhhawk@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.affiliation: Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. tjhhawk@163.com.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China. qihuishi@sjtu.edu.cn and State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University, Shanghai, China and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Shi
mesh_terms: D002452:Cell Count; D018929:Cell Culture Techniques; D006801:Humans; D018189:Immunomagnetic Separation; D008175:Lung Neoplasms / Q000175:diagnosis*; D046210:Microfluidic Analytical Techniques; D009360:Neoplastic Cells, Circulating