title: Clostridium kogasensis sp. nov., a novel member of the genus Clostridium, isolated from soil under a corroded gas pipeline.
abstract: Two bacterial strains, YHK0403(T) and YHK0508, isolated from soil under a corroded gas pipe line, were revealed as Gram-negative, obligately anaerobic, spore-forming and mesophilic bacteria. The cells were rod-shaped and motile by means of peritrichous flagella. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolates were members of the genus Clostridium and were the most closely related to Clostridium scatologenes KCTC 5588(T) (95.8% sequence similarity), followed by Clostridium magnum KCTC 15177(T) (95.8%), Clostridium drakei KCTC 5440(T) (95.7%) and Clostridium tyrobutyricum KCTC 5387(T) (94.9%). The G + C contents of the isolates were 29.6 mol%. Peptidoglycan in the cell wall was of the A1γ type with meso-diaminopimelic acid. The major polar lipid was diphosphatidylglycerol (DPG), and other minor lipids were revealed as phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unknown glycolipids (GL1 and GL2), an unknown aminoglycolipid (NGL), two unknown aminophospholipids (PN1 and PN2) and four unknown phospholipids (PL1 to PL4). Predominant fatty acids were C16:0 and C16:1cis9 DMA. The major end products from glucose fermentation were identified as butyrate (12.2 mmol) and acetate (9.8 mmol). Collectively, the results from a wide range of phenotypic tests, chemotaxonomic tests, and phylogenetic analysis indicated that the two isolates represent novel species of the genus Clostridium, for which the name Clostridium kogasensis sp. nov. (type strain, YHK0403(T) = KCTC 15258(T) = JCM 18719(T)) is proposed.
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Yeseul
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul, 110-749, Republic of Korea.
authors.forename: Seok-Seong
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Jayoung
authors.initials: J
authors.lastname: Paek
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Tae Eun
authors.initials: TE
authors.lastname: Jin
authors.affiliation: R&D Division, Korea Gas Corporation, 638-1 Il-dong, Ansan, Gyeonggi-Do 426-790, Republic of Korea.
authors.forename: Hong Seok
authors.initials: HS
authors.lastname: Song
authors.affiliation: Vitabio, Inc., Daejeon, 305-500, Republic of Korea.
authors.forename: Hongik
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Microbiology & Molecular Biology, Chungnam National University, Daejeon 305- 764, Republic of Korea.
authors.forename: Hee-Moon
authors.initials: HM
authors.lastname: Park
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea. Electronic address: yhchang@kribb.re.kr.
authors.forename: Young-Hyo
authors.initials: YH
authors.lastname: Chang
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000693:Anaerobiosis; D001482:Base Composition; D002087:Butyrates / Q000378:metabolism; D002308:Cardiolipins / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D003013:Clostridium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D003343:Corrosion; D003960:Diaminopimelic Acid / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D005407:Flagella / Q000737:chemistry* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007501:Iron / Q000737:chemistry; D010457:Peptidoglycan / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002087:Butyrates; D002308:Cardiolipins; D005227:Fatty Acids; D010457:Peptidoglycan; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; C483858:phosphatidylethanolamine; D003960:Diaminopimelic Acid; D007501:Iron; D005947:Glucose; D019342:Acetic Acid
keywords: Anaerobic; Clostridium kogasensis nov.; New species; Phylogenetic