title: Differentiation with elaidate tends to impair insulin-dependent glucose uptake and GLUT4 translocation in 3T3-L1 adipocytes.
abstract: Development of type 2 diabetes mellitus and insulin resistance is associated with a quality of dietary fatty acids such as saturated and unsaturated fatty acids. Dietary fatty acids also include transform of unsaturated fatty acids and intake of transform of oleate (elaidate) is associated with cardiovascular disease. However, little is known about the roles of elaidate in insulin responsiveness. We show here that elaidate impairs insulin-dependent glucose uptake in adipocytes. Differentiation with 10 μM elaidate, which is close to physiological plasma concentration, reduces insulin-dependent glucose uptake. Furthermore, insulin-dependent GLUT4 translocation is disturbed in adipocytes differentiated with elaidate. In addition, analysis of lipolysis and gene expression shows that deteriorative effects of elaidate on insulin responsiveness are limited but not general. Thus, our findings reveal that differentiation with elaidate tends to affect insulin-dependent glucose uptake through alternation of GLUT4 translocation from cytosol to the plasma membrane.
authors.affiliation: a Department of Molecular Physiology and Pathology , School of Pharma-Sciences, Teikyo University , Tokyo , Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ishibashi
authors.affiliation: a Department of Molecular Physiology and Pathology , School of Pharma-Sciences, Teikyo University , Tokyo , Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Nehashi
authors.affiliation: a Department of Molecular Physiology and Pathology , School of Pharma-Sciences, Teikyo University , Tokyo , Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Oshima
authors.affiliation: a Department of Molecular Physiology and Pathology , School of Pharma-Sciences, Teikyo University , Tokyo , Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohkura
authors.affiliation: a Department of Molecular Physiology and Pathology , School of Pharma-Sciences, Teikyo University , Tokyo , Japan.
authors.forename: Gen-Ichi
authors.initials: G
authors.lastname: Atsumi
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D005227:Fatty Acids / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D051379:Mice; D019301:Oleic Acid / Q000494:pharmacology*; D009829:Oleic Acids; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D051275:Glucose Transporter Type 4; D007328:Insulin; D009829:Oleic Acids; C495693:Slc2a4 protein, mouse; D012710:Serum Albumin, Bovine; D019301:Oleic Acid; C011459:elaidic acid; D005947:Glucose
keywords: Adipocytes; GLUT4 translocation; elaidate; glucose uptake; insulin; trans-fatty acids