title: Natural products-prompted chemical biology: phenotypic screening and a new platform for target identification.
abstract: Covering: 1993 to 2016The exploitation of small molecules from natural sources, such as microbial metabolites, has contributed to the discovery of not only new drugs but also new research tools for chemical biology. My research team has discovered several novel bioactive small molecules using in vivo cell-based phenotypic screening, and has investigated their modes of action using chemical genetics and chemical genomics. This highlight focuses on our recent discoveries and chemical genetics approaches for bioactive microbial metabolites that target cancer cells, the cancer microenvironment and cell membrane signalling. In addition, the development of two new platforms, 5-sulfonyl tetrazole-based and thiourea-modified amphiphilic lipid-based probe technologies, to identify the cellular targets of these molecules is also discussed.
authors.affiliation: Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. scseigyo-hisyo@pharm.kyoto-u.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kakeya
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology; D015195:Drug Design; D055808:Drug Discovery; D023281:Genomics; D006801:Humans; D015394:Molecular Structure; D013777:Tetrazoles / Q000737:chemistry / Q000494:pharmacology; D013890:Thiourea / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001688:Biological Products; D013777:Tetrazoles; D013890:Thiourea