title: Rapid Detection of Ricin in Serum Based on Cu-Chelated Magnetic Beads Using Mass Spectrometry.
abstract: The protein toxin ricin obtained from castor bean plant (Ricinus communis) seeds is a potent biological warfare agent due to its ease of availability and acute toxicity. In this study, we demonstrated a rapid and simple method to detect ricin in serum in vitro. The ricin was mixed with serum and digested by trypsin, then all the peptides were efficiently extracted using Cu-chelated magnetic beads and were detected with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The specific ricin peptides were identified by Nanoscale Ultra Performance liquid chromatography coupled to tandem mass spectrometry according to their sequences. The assay required 2.5 hours, and a characteristic peptide could be detected down to 4 ng/μl and used as a biomarker to detect ricin in serum. The high sensitivity and simplicity of the procedure makes it valuable in clinical practice. Graphical Abstract ᅟ.
authors.affiliation: The Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China, 100850.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Zhao
authors.affiliation: The School of Pharmacy, Jilin University, Changchun, China, 130021.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.affiliation: The Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China, 100850.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Wang
authors.affiliation: The Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China, 100850.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: The Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China, 100850.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: The Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China, 100850. hk@proteomics.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: He
authors.affiliation: The Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing, China, 100850. nwang@ncba.ac.cn.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D002614:Chelating Agents / Q000737:chemistry*; D002619:Chemical Warfare Agents / Q000032:analysis / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003300:Copper / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry*; D015203:Reproducibility of Results; D012276:Ricin / Q000032:analysis / Q000097:blood*; D012278:Ricinus / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D002614:Chelating Agents; D002619:Chemical Warfare Agents; D003300:Copper; D012276:Ricin
keywords: Cu-Magbeads; Mass spectrometry; Rapid detection; Ricin