title: Full-length M and L RNA sequences of tospovirus isolate 2009-GZT, which causes necrotic ringspot on tomato in China.
abstract: The virus isolate 2009-GZT, collected from tomato in Guizhou province of China, was identified as a new member of the genus Tospovirus based on its S RNA sequence. Because its provisional name, "tomato necrotic spot virus" (TNSV), was identical to an already existing member of the genus Ilarvirus, 2009-GZT was renamed "tomato necrotic spot-associated virus" (TNSaV). In this study, the full-length sequences of the genomic M and L RNAs of TNSaV were determined and analyzed. The M RNA has 4,773 nucleotides (nt), encoding the NSm protein of 309 aa (34.4 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1123 aa (128 kDa) in the viral complementary (vc) strand. The NSm and Gn/Gc of TNSaV share the highest aa sequence identity (86.2 % and 86.9 %, respectively) with those of tomato zonate spot virus. The L RNA contains 8,908 nt and codes for the putative RNA-dependent RNA polymerase (RdRp) of 2885 aa (332 kDa) in the vc strand. The RdRp of TNSaV shares the highest aa sequence identity (85.2 %) with that of calla lily chlorotic spot virus (CCSV). Serological assays showed that TNSaV cross-reacts with rabbit antisera against watermelon silver mottle virus (WSMoV) NP and CCSV NP, indicating that TNSaV is a member of the WSMoV serogroup.
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.|Yunnan Provincial Key Lab of Agricultural Biotechnology, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.|Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.
authors.forename: Kuanyu
authors.initials: K
authors.lastname: Zheng
authors.affiliation: HongyunHonghe Group Limited Company, 181# Hongjin Road, Kunming, 650202, Yunnan, People's Republic of China.
authors.forename: Hongguang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.|Yunnan Provincial Key Lab of Agricultural Biotechnology, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.|Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.
authors.forename: Yueyan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan.
authors.forename: Tsung-Chi
authors.initials: TC
authors.lastname: Chen
authors.affiliation: Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan.
authors.forename: Shyi-Dong
authors.initials: SD
authors.lastname: Yeh
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.|Yunnan Provincial Key Lab of Agricultural Biotechnology, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.|Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China.
authors.forename: Zhongkai
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China. dongjhn@126.com.|Yunnan Provincial Key Lab of Agricultural Biotechnology, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China. dongjhn@126.com.|Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9# Xueyun Rd, Wuhua prefecture, Kunming, 650223, Yunnan, People's Republic of China. dongjhn@126.com.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Dong
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018749:RNA, Plant / Q000235:genetics*; D017865:Tospovirus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D018749:RNA, Plant