title: Neutrophil extracellular Taps play an important role in clearance of Streptococcus suis in vivo.
abstract: Streptococcus suis infection induces formation of neutrophil extracellular traps (NETs) in vitro; however, the contribution of NETs-mediated killing to the pathogenesis of S. suis in vivo is yet to be elicited. The findings of the present study indicated that extracellular DNA fiber can be induced in a murine model in response to S. suis infection. A nuclease that destroys their structure was used to evaluate the role of NETs on S. suis infection. Treatment with nuclease resulted in a greater bacteria load and higher serum TNF-α concentrations in response to S. suis infection, indicating that NETs structure played an essential role in S. suis clearance and inflammation. Furthermore, nuclease treatment resulted in more severe clinical signs during and higher mortality from S. suis infection. These findings indicated that NETs structure contributes to protection against S. suis infection.
authors.affiliation: National Key Laboratory of Agricultural Microbiology.|College of Veterinary Medicine, Huazhong Agricultural University.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: National Key Laboratory of Agricultural Microbiology.|College of Veterinary Medicine, Huazhong Agricultural University.
authors.forename: Lan
authors.initials: L
authors.lastname: Lin
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University.
authors.forename: Lei
authors.initials: L
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University.|Cooperative Innovation Center for Sustainable Pig Production.|Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, Hubei, 430070, China.
authors.forename: Anding
authors.initials: A
authors.lastname: Zhang
mesh_terms: D000818:Animals; D058491:Bacterial Load; D003851:Deoxyribonucleases / Q000494:pharmacology; D065206:Extracellular Traps / Q000276:immunology*; D005260:Female; D054884:Host-Pathogen Interactions; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000276:immunology*; D009696:Nucleic Acids / Q000097:blood; D011897:Random Allocation; D013290:Streptococcal Infections / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology / Q000628:therapy; D017011:Streptococcus suis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D009696:Nucleic Acids; D014409:Tumor Necrosis Factor-alpha; D003851:Deoxyribonucleases
keywords: Streptococcus suis; bacterial clearance; neutrophil extracellular traps