title: A two-photon off-on fluorescence probe for imaging thiols in live cells and tissues.
abstract: A two-photon (TP) fluorescence imaging probe (Z1) was designed to detect biothiols through a photoinduced electron transfer pathway utilizing N-butyl-naphthalimide as the fluorophore and 2,4-dinitrobenzene-sulfonyl as the responsive group, which were linked together by piperazine. The synthesized Z1 displayed high selectivity to biothiols, significant fluorescence off-on properties, and a marked two-photon absorption cross section (δ = 110 GM). Moreover, Z1 showed good biocompatibility and insensitivity toward changes in the biologically relevant pH range (7.2-8.4), which enabled the utilization of Z1 to monitor biothiol levels not only in live cells but also in tissues at depths of 50-250 μm.
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. zhanghx@lzu.edu.cn.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Wenyan
authors.initials: W
authors.lastname: Zan
authors.forename: Jianjian
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Fengchao
authors.initials: F
authors.lastname: Qi
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yao
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009369:Neoplasms / Q000737:chemistry* / Q000473:pathology*; D017785:Photons; D011789:Quantum Theory; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds