title: Synthesis of Keratin-based Nanofiber for Biomedical Engineering.
abstract: Electrospinning, due to its versatility and potential for applications in various fields, is being frequently used to fabricate nanofibers. Production of these porous nanofibers is of great interest due to their unique physiochemical properties. Here we elaborate on the fabrication of keratin containing poly (ε-caprolactone) (PCL) nanofibers (i.e., PCL/keratin composite fiber). Water soluble keratin was first extracted from human hair and mixed with PCL in different ratios. The blended solution of PCL/keratin was transformed into nanofibrous membranes using a laboratory designed electrospinning set up. Fiber morphology and mechanical properties of the obtained nanofiber were observed and measured using scanning electron microscopy and tensile tester. Furthermore, degradability and chemical properties of the nanofiber were studied by FTIR. SEM images showed uniform surface morphology for PCL/keratin fibers of different compositions. These PCL/keratin fibers also showed excellent mechanical properties such as Young's modulus and failure point. Fibroblast cells were able to attach and proliferate thus proving good cell viability. Based on the characteristics discussed above, we can strongly argue that the blended nanofibers of natural and synthetic polymers can represent an excellent development of composite materials that can be used for different biomedical applications.
authors.affiliation: Department of Chemical, Biological and Bioengineering, North Carolina A&T State University.
authors.forename: Zanshe S
authors.initials: ZS
authors.lastname: Thompson
authors.affiliation: Department of Chemical, Biological and Bioengineering, North Carolina A&T State University.
authors.forename: Nava P
authors.initials: NP
authors.lastname: Rijal
authors.affiliation: Department of Chemical, Biological and Bioengineering, North Carolina A&T State University.
authors.forename: David
authors.initials: D
authors.lastname: Jarvis
authors.affiliation: Department of Chemical, Biological and Bioengineering, North Carolina A&T State University.
authors.forename: Angela
authors.initials: A
authors.lastname: Edwards
authors.affiliation: Department of Chemical, Biological and Bioengineering, North Carolina A&T State University; NSF ERC for Revolutionizing Metallic Biomaterials, North Carolina A&T State University; nbhattar@ncat.edu.
authors.forename: Narayan
authors.initials: N
authors.lastname: Bhattarai
mesh_terms: D001698:Biomedical Engineering / Q000379:methods*; D002470:Cell Survival; D055119:Elastic Modulus; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D007633:Keratins / Q000138:chemical synthesis*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D054457:Tissue Scaffolds
chemical_list: D007633:Keratins