title: Exploring the effects of the atherosclerosis progression and the choice of affected arteries in the design of experiments with Apolipoprotein E-deficient mice.
abstract: The objective of this study is to explore the longitudinal progression of atherosclerosis and the correlation between methods to measure the lesion in apolipoprotein E-deficient mice. Atherosclerosis progression was assessed by measurements of foam cell-rich depositions in their proximal aortas, and/or in surgically excised arteries, to assess the histological luminal narrowing. A longitudinal study was performed by comparing the values for carotid, aorta, and femoral and iliac arteries using common histological techniques. There were no significant differences in progression between different arteries, but correlation with the classical measurement of atherosclerosis in the aortic root was poor. Each laboratory requires specific standardization. Carotid arteries were sensitive to atherosclerosis in these mice, and progression was exponential. In conclusion, morphometric data show the importance of the choice of the duration of treatment, the appropriate controls, and the age at which to begin the experiments.
authors.affiliation: Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, carrer Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Riera-Borrull
authors.affiliation: Servei de Cirurgia General i de l'Aparell Digestiu, Hospital Universitari Sant Joan, Universitat Rovira i Virgili, carrer Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Fàtima
authors.initials: F
authors.lastname: Sabench
authors.affiliation: Servei de Cirurgia General i de l'Aparell Digestiu, Hospital Universitari Sant Joan, Universitat Rovira i Virgili, carrer Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: del Castillo
authors.affiliation: Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, carrer Sant Llorenç 21, 43201 Reus, Spain. Electronic address: jcamps@grupsagessa.com.
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
authors.affiliation: Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, carrer Sant Llorenç 21, 43201 Reus, Spain. Electronic address: jorge.joven@urv.cat.
authors.forename: Jorge
authors.initials: J
authors.lastname: Joven
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology*; D001057:Apolipoproteins E / Q000235:genetics*; D050197:Atherosclerosis / Q000235:genetics / Q000473:pathology*; D002339:Carotid Arteries / Q000473:pathology*; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005263:Femoral Artery / Q000473:pathology; D005487:Foam Cells / Q000378:metabolism; D007083:Iliac Artery / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors
chemical_list: D001057:Apolipoproteins E
keywords: Animal models; Apolipoprotein E; Apolipoproteína E; Aterosclerosis; Atherosclerosis; Enfermedad arterial periférica; Modelos animales; Peripheral artery disease