title: Cell Treatment for Stroke in Type Two Diabetic Rats Improves Vascular Permeability Measured by MRI.
abstract: Treatment of stroke with bone marrow stromal cells (BMSC) significantly enhances brain remodeling and improves neurological function in non-diabetic stroke rats. Diabetes is a major risk factor for stroke and induces neurovascular changes which may impact stroke therapy. Thus, it is necessary to test our hypothesis that the treatment of stroke with BMSC has therapeutic efficacy in the most common form of diabetes, type 2 diabetes mellitus (T2DM). T2DM was induced in adult male Wistar rats by administration of a high fat diet in combination with a single intraperitoneal injection (35mg/kg) of streptozotocin. These rats were then subjected to 2h of middle cerebral artery occlusion (MCAo). T2DM rats received BMSC (5x106, n = 8) or an equal volume of phosphate-buffered saline (PBS) (n = 8) via tail-vein injection at 3 days after MCAo. MRI was performed one day and then weekly for 5 weeks post MCAo for all rats. Compared with vehicle treated control T2DM rats, BMSC treatment of stroke in T2DM rats significantly (p<0.05) decreased blood-brain barrier disruption starting at 1 week post stroke measured using contrast enhanced T1-weighted imaging with gadopentetate, and reduced cerebral hemorrhagic spots starting at 3 weeks post stroke measured using susceptibility weighted imaging, although BMSC treatment did not reduce the ischemic lesion volumes as demarcated by T2 maps. These MRI measurements were consistent with histological data. Thus, BMSC treatment of stroke in T2DM rats initiated at 3 days after stroke significantly reduced ischemic vascular damage, although BMSC treatment did not change infarction volume in T2DM rats, measured by MRI.
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.
authors.forename: Guangliang
authors.initials: G
authors.lastname: Ding
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.
authors.forename: Jieli
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.|Department of Physics, Oakland University, Rochester, Michigan, 48309, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Chopp
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.
authors.forename: Lian
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.|Department of Neurology, Tianjin Medical University General Hospital, Tianjin, 300052, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Cui
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.
authors.forename: Siamak P N
authors.initials: SP
authors.lastname: Davarani
authors.affiliation: Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, Michigan, 48202, United States of America.
authors.forename: Quan
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism / Q000473:pathology; D002199:Capillary Permeability; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002543:Cerebral Hemorrhage / Q000175:diagnosis / Q000209:etiology; D003921:Diabetes Mellitus, Experimental; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D051381:Rats; D020521:Stroke / Q000150:complications / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism* / Q000628:therapy