title: [Culture effects of mouse induced pluripotent stem cells by three-dimensional films in vitro].
abstract: OBJECTIVE
  To observe the proliferation and differentiation capacities of mouse induced pluripotent stem cells (miPSCs) cultured in 3-hydroxybutyrate-co-3-hydroxyhexanoate(PHBHHx) three-dimensional films three-dimension films for the purpose of finding a suitable polymeric biomaterials for forming myocardial patches.
  
  
  METHODS
  miPSCs were recovered, passaged, cultured and identified, then miPSCs divided into the experimental and control groups. MiPSCs in the experimental group were cultured with PHBHHx three-dimension films. MiPSCs in the control group were cultured with traditional culture dish. Stem cell culture medium or differential medium were added to miPSCs to detecte cell vitality by CCK-8 after 72 hours or to measure the cTnT expression of miPSCs through immunofluorescence or the cTnT expression quantity through flow cytometry after 15 days.
  
  
  RESULTS
  Cell activity assay showed that the absorbance values were 0.836 ± 0.038 in the experimental group, 0.312 ± 0.004 in the control group (P<0.05). Scanning electron microscope (SEM) observation showed that miPSCs grew well on the PHBHHx dimensional films with normal shape. Immunofluorescence results demonstrated positive cTnT expression in both groups and flow cytometry measured cTnT expression was (60.32 ± 1.76)% in the experimental group and (47.54 ± 1.46)% in the control group (P<0.05).
  
  
  CONCLUSIONS
  miPSCs can survive, proliferate and differentiate on PHBHHx dimensional films. miPSCs proliferation and differentiation capacities are significantly higher in PHBHHx three-dimensional films culture compared with the traditional cell culture.
authors.affiliation: Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China; Email: wesleymu@hotmail.com.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Mu
authors.affiliation: Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Jianqun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Cardiac Surgery, Capital Medical University, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Bo
mesh_terms: D020155:3-Hydroxybutyric Acid; D000818:Animals; D001672:Biocompatible Materials; D002208:Caproates; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D057026:Induced Pluripotent Stem Cells; D051379:Mice
chemical_list: D001672:Biocompatible Materials; D002208:Caproates; C045051:3-hydroxyhexanoic acid; D020155:3-Hydroxybutyric Acid