title: New insights into the phenotype of human dendritic cell populations.
abstract: HLDA10 is the Tenth Human Leukocyte Differentiation Antigen (HLDA) Workshop. The HLDA Workshops provide a mechanism to allocate cluster of differentiation (CD) nomenclature by engaging in interlaboratory studies. As the host laboratory, we invited researchers from national and international academic and commercial institutions to submit monoclonal antibodies (mAbs) to human leukocyte surface membrane molecules, particularly those that recognised molecules on human myeloid cell populations and dendritic cells (DCs). These mAbs were tested for activity and then distributed as a blinded panel to 15 international laboratories to test on different leukocyte populations. These populations included blood DCs, skin-derived DCs, tonsil leukocytes, monocyte-derived DCs, CD34-derived DCs, macrophage populations and diagnostic acute myeloid leukaemia and lymphoma samples. Each laboratory was provided with enough mAb to perform five repeat experiments. Here, we summarise the reactivity of different mAb to 68 different cell-surface molecules expressed by human myeloid and DC populations. Submitted mAbs to some of the molecules were further validated to collate data required to designate a formal CD number. This collaborative process provides the broader scientific community with an invaluable data set validating mAbs to leukocyte-surface molecules.
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Concord, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Georgina Jane
authors.initials: GJ
authors.lastname: Clark
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute , Concord, Sydney, NSW, Australia.
authors.forename: Fiona
authors.initials: F
authors.lastname: Kupresanin
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Concord, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Phillip Dieter
authors.initials: PD
authors.lastname: Fromm
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute , Concord, Sydney, NSW, Australia.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Ju
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute , Concord, Sydney, NSW, Australia.
authors.forename: Leticia
authors.initials: L
authors.lastname: Muusers
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Concord, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Pablo Alejandro
authors.initials: PA
authors.lastname: Silveira
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute , Concord, Sydney, NSW, Australia.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Elgundi
authors.affiliation: Sydney Medical School, University of Sydney , Sydney, NSW, Australia.
authors.forename: Robin Edward
authors.initials: RE
authors.lastname: Gasiorowski
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Concord, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Michael Stefan
authors.initials: MS
authors.lastname: Papadimitrious
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Concord, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Christian
authors.initials: C
authors.lastname: Bryant
authors.affiliation: Anatomical Pathology Department, Concord Hospital , Sydney, NSW, Australia.
authors.forename: Kenneth Maan Liong
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Anatomical Pathology Department, Concord Hospital , Sydney, NSW, Australia.
authors.forename: Candice Jane
authors.initials: CJ
authors.lastname: Clarke
authors.affiliation: Adult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center , New York, NY, USA.
authors.forename: James William
authors.initials: JW
authors.lastname: Young
authors.affiliation: Adult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center , New York, NY, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Chan
authors.affiliation: Centre for Virus Research, Westmead Millennium Institute , Sydney, NSW, Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Harman
authors.affiliation: Centre for Virus Research, Westmead Millennium Institute , Sydney, NSW, Australia.
authors.forename: Rachel
authors.initials: R
authors.lastname: Botting
authors.affiliation: Inflammatory Bowel Disease Department, CIBEREHD, Hospital Clínic de Barcelona , Barcelona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Cabezón
authors.affiliation: Inflammatory Bowel Disease Department, CIBEREHD, Hospital Clínic de Barcelona , Barcelona, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Benitez-Ribas
authors.affiliation: School of Biological Sciences, The University of Auckland, Auckland, New Zealand and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland , Auckland, New Zealand.
authors.forename: Anna Elizabeth Stella
authors.initials: AE
authors.lastname: Brooks
authors.affiliation: School of Biological Sciences, The University of Auckland, Auckland, New Zealand and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland , Auckland, New Zealand.
authors.forename: Phillip Rodney
authors.initials: PR
authors.lastname: Dunbar
authors.affiliation: Dendritic Cell Research, ANZAC Research Institute, Concord, Sydney, NSW, Australia; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
authors.forename: Derek Nigel John
authors.initials: DN
authors.lastname: Hart