title: A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.
abstract: The Schizosaccharomyces pombe MBF complex activates the transcription of genes required for DNA synthesis and S phase. The MBF complex contains several proteins, including the core components Cdc10, Res1 and Res2, the co-repressor proteins Yox1 and Nrm1 and the co-activator Rep2. It has recently been shown how MBF is regulated when either the DNA damage or the DNA synthesis checkpoints are activated. However, how MBF is regulated in a normal unperturbed cell cycle is still not well understood. We have set up a genome-wide genomic screen searching for global regulators of MBF. We have crossed our knock-out collection library with a reporter strain that allows the measurement of MBF activity in live cells by flow cytometry. We confirm previously known regulators of MBF and show that COP9/signalosome and tRNA methyltransferases also regulate MBF activity.
authors.affiliation: a Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra , Barcelona , Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Gaspa
authors.affiliation: a Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra , Barcelona , Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: González-Medina
authors.affiliation: a Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra , Barcelona , Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Hidalgo
authors.affiliation: a Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra , Barcelona , Spain.
authors.forename: José
authors.initials: J
authors.lastname: Ayté
mesh_terms: D000075686:COP9 Signalosome Complex; D059585:G1 Phase Cell Cycle Checkpoints; D015966:Gene Expression Regulation, Fungal; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D016681:Genome, Fungal; D008745:Methylation; D046912:Multiprotein Complexes / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D016196:S Phase; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D046912:Multiprotein Complexes; D029702:Schizosaccharomyces pombe Proteins; D012343:RNA, Transfer; D010447:Peptide Hydrolases; D000075686:COP9 Signalosome Complex
keywords: COP9; Cdc22-YFP; Elongator; G1/S transition; MBF complex; S. pombe