title: Detection EGFR exon 19 status of lung cancer patients by DNA electrochemical biosensor.
abstract: Epidermal growth factor receptor (EGFR) exon 19 mutation status is a very important prediction index for tyrosine kinase inhibitors (TKIs) therapy. In this paper, we constructed a superior selective sandwich-type electrochemical biosensor to detect in-frame deletions in exon 19 of EGFR in real samples of patients with non-small cell lung carcinoma. Based on the characteristics of different hybridization efficiency in different hybridization phase conditions, different region around EGFR exon 19 deletion hotspots was selected to design DNA probes to improve biosensor performance. The results confirm that alteration of deletion location in target deliberately according to different hybridization phase is able to improve selectivity of sandwich-type DNA biosensor. Satisfactory discrimination ability can be achieved when the deletions are located in the capture probe interaction region. In order to improve efficiency of ssDNA generation from dsDNA, we introduce Lambda exonuclease (λ-exo) to sandwich-type biosensor system. EGFR exon 19 statuses of clinical real samples from lung cancer patients can be discriminated successfully by the proposed method. Our research would make the electrochemical biosensor be an excellent candidate for EGFR detection for lung cancer patients.
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, PR China.
authors.forename: Xiong-Wei
authors.initials: XW
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, PR China. Electronic address: shirly0409@163.com.
authors.forename: Xiu-Hua
authors.initials: XH
authors.lastname: Weng
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, PR China.
authors.forename: Chang-Lian
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, PR China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Lin
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, Fujian 350004, PR China.
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, Fujian 350004, PR China. Electronic address: chenandhu@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, Fujian 350004, PR China. Electronic address: xhl1963@sina.com.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Lin
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D004247:DNA / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods*; D066246:ErbB Receptors / Q000235:genetics*; D005091:Exons; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation; D017384:Sequence Deletion
chemical_list: D004247:DNA; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Detection; Electrochemical biosensor; Epidermal growth factor receptor; In-frame deletions; Lambda exonuclease; Non-small cell lung carcinoma