title: Association of XPF Levels and Genetic Polymorphism with Susceptibility to Ischemic Stroke.
abstract: The xeroderma pigmentosum group F (XPF) gene participates in the pathophysiological process of ischemic stroke, and XPF polymorphisms might be associated with ischemic stroke susceptibility. This study aimed to investigate XPF messenger RNA (mRNA) levels in peripheral blood mononuclear cells and protein levels in plasma and to analyze the 30028T/C polymorphism (rs1799801) in ischemic stroke patients and controls. Levels of both mRNA and protein in ischemic stroke patients were significantly lower than in controls (P < 0.05). The C allele of the 30028T/C polymorphism significantly increased the risk of ischemic stroke (OR = 1.512, 95 % CI = 1.219-1.875). The CT and CC/CT genotypes of 30028T/C were observed significantly more frequently in ischemic stroke patients than in controls (CT: OR = 1.916, 95 % CI = 1.446-2.539;
  OR = 1.877, 95 % CI = 1.427-2.468). Similar results were obtained after adjusting for age, gender, and smoking status. Additionally, XPF plasma protein levels were significantly decreased in the CC/CT genotype compared with the TT genotype (P = 0.025). These data indicate that XPF might play an important role in the pathophysiological process of ischemic stroke, and the 30028T/C polymorphism might be associated with ischemic stroke susceptibility in a Chinese Han population.
authors.affiliation: Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Deng
authors.affiliation: Department of Criminal Science and Technology, Chongqing Police College, Chongqing, 401331, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China.
authors.forename: Sun-Xian
authors.initials: SX
authors.lastname: Wang
authors.affiliation: Department of Forensic Medicine, North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China. xyun2005@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Physiology, North Sichuan Medical College, Nanchong, Sichuan, 637000, People's Republic of China. 1099389691@qq.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
mesh_terms: D000368:Aged; D002545:Brain Ischemia / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D020521:Stroke / Q000235:genetics* / Q000473:pathology
chemical_list: D004268:DNA-Binding Proteins; C095888:xeroderma pigmentosum group F protein
keywords: Genetic susceptibility; Ischemic stroke; Single-nucleotide polymorphisms; Xeroderma pigmentosum group F (XPF)