title: Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains.
abstract: In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped by molecular approaches and 45 of the representative strains were tested for selected important oenological properties including ethanol, sulfur dioxide and osmotic stress tolerance, intensity of flocculation and desirable enzymatic activities. Their ability to produce and utilize acetic/malic acid was examined as well; in addition, H2S production as an undesirable property was screened. The oenological characteristics of indigenous isolates were compared to a commercially available S. cerevisiae BS6 strain, which is commonly used as the starter culture. Finally, some indigenous strains coming from organically treated grape berries were chosen for their promising oenological properties and these strains will be used as the starter culture, because application of a selected indigenous S. cerevisiae strain can enhance the regional character of the wines.
authors.affiliation: Brno University of Technology, Faculty of Chemistry, Department of Food Science and Biotechnology, Purkyňova 464/118, 612 00 Brno, Czech Republic. Electronic address: suranska.h@gmail.com.
authors.forename: Hana
authors.initials: H
authors.lastname: Šuranská
authors.affiliation: Brno University of Technology, Faculty of Chemistry, Department of Food Science and Biotechnology, Purkyňova 464/118, 612 00 Brno, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Vránová
authors.affiliation: Brno University of Technology, Faculty of Chemistry, Department of Food Science and Biotechnology, Purkyňova 464/118, 612 00 Brno, Czech Republic.
authors.forename: Jiřina
authors.initials: J
authors.lastname: Omelková
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D001422:Bacterial Adhesion; D018153:Czech Republic; D016172:DNA Fingerprinting; D004361:Drug Tolerance; D000431:Ethanol / Q000633:toxicity; D006862:Hydrogen Sulfide / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D009997:Osmotic Pressure; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D013312:Stress, Physiological; D013458:Sulfur Dioxide / Q000633:toxicity; D027843:Vitis / Q000382:microbiology*
chemical_list: D008293:Malates; D013458:Sulfur Dioxide; D000431:Ethanol; C030298:malic acid; D019342:Acetic Acid; D006862:Hydrogen Sulfide
keywords: ITS-PCR-RFLP; Interdelta PCR typing; Oenological properties; Saccharomyces genus; Species-specific primers