title: Transportan 10 improves the anticancer activity of cisplatin.
abstract: The aim of this paper was to examine whether cell-penetrating peptides (CPPs) such as transportan 10 (TP10) or protein transduction domain (PTD4) may improve the anticancer activity of cisplatin (cPt). The complexes of TP10 or PTD4 with cPt were used in the experiments. They were carried out on two non-cancer (HEK293 (human embryonic kidney) and HEL299 (human embryo lung)) and two cancer (HeLa (human cervical cancer) and OS143B (human osteosarcoma 143B)) cell lines. Both complexes were tested (MTT assay) with respect to their anticancer or cytotoxic actions. TAMRA (fluorescent dye)-stained preparations were visualized in a fluorescence microscope. The long-term effect of TP10 + cPt and its components on non-cancer and cancer cell lines was observed in inverted phase contrast microscopy. In the MTT test (cell viability assay), the complex of TP10 + cPt produced a more potent effect on the cancer cell lines (HeLa, OS143B) in comparison to that observed after separate treatment with TP10 or cPt. At the same time, the action of the complex and its components was rather small on non-cancer cell lines. On the other hand, a complex of another CPP with cPt, i.e., PTD4 + cPt, was without a significant effect on the cancer cell line (OS143B). The images of the fluorescent microscopy showed TAMRA-TP10 or TAMRA-TP10 + cPt in the interior of the HeLa cells. In the case of TAMRA-PTD4 or TAMRA-PTD4 + cPt, only the first compound was found inside the cancer cell line. In contrast, none of the tested compounds gained access to the interior of the non-cancer cells (HEK293, HEL299). Long-term incubation with the TP10 + cPt (estimated by inverted phase contrast microscopy) lead to an enhanced action of the complex on cell viability (decrease in the number of cells and change in their morphology) as compared with that produced by each single agent. With regard to the tested CPPs, only TP10 improved the anticancer activity of cisplatin if both compounds were used in the form of a complex. Additionally, the complex was relatively safe for non-cancer cells. What is more, TP10 also produced an anticancer effect on HeLa and OS143B cell lines.
authors.affiliation: Department of Pharmacology, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Rusiecka
authors.initials: R
authors.lastname: Izabela
authors.affiliation: Department of Chemistry, University of Gdańsk, Gdańsk, Poland.
authors.forename: Ruczyński
authors.initials: R
authors.lastname: Jarosław
authors.affiliation: Department of Chemistry, University of Gdańsk, Gdańsk, Poland.
authors.forename: Alenowicz
authors.initials: A
authors.lastname: Magdalena
authors.affiliation: Department of Chemistry, University of Gdańsk, Gdańsk, Poland.
authors.forename: Rekowski
authors.initials: R
authors.lastname: Piotr
authors.affiliation: Department of Pharmacology, Medical University of Gdańsk, Gdańsk, Poland. ikocic@gumed.edu.pl.
authors.forename: Kocić
authors.initials: K
authors.lastname: Ivan
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000494:pharmacology*; D012235:Rhodamines / Q000737:chemistry / Q000494:pharmacology
chemical_list: C114557:5-carboxytetramethylrhodamine succinimidyl ester; D000970:Antineoplastic Agents; D057846:Cell-Penetrating Peptides; D011993:Recombinant Fusion Proteins; D012235:Rhodamines; C586078:transportan-10; D002945:Cisplatin
keywords: Cell-penetrating peptides (CPPs); Click chemistry; HEK293 cell line; HEL299 cell line; HeLa cell line; OS143B cell line; Transportan 10 (TP10)