title: Assessment of Expression of Genes Coding GABAA Receptors during Chronic and Acute Intoxication of Laboratory Rats with Ethanol.
abstract: Expression of genes encoding the individual subunits of ionotropic GABAA receptor was assessed after acute and chronic intoxication of rats with ethanol. The chronic 1-month-long exposure to ethanol signifi cantly decreased (by 38%) expression of Gabrb1 gene in the hippocampus. Acute exposure to ethanol elevated expression of genes Gabrb1 (by 1.7 times), Gabra1 (by 3.8 times), and Gabra4 (by 6.5 times), although it diminished expression of Gabra2 gene by 1.4 times. In preliminarily alcoholized rats, acute intoxication with ethanol enhanced expression of genes Gabrb1 and Gabra5 by 1.7 and 8.7 times, respectively. There was neither acute nor chronic effect of ethanol on expression of gene Gabra3.
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia. dunats@rambler.ru.
authors.forename: N S
authors.initials: NS
authors.lastname: Osechkina
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia.
authors.forename: M B
authors.initials: MB
authors.lastname: Ivanov
authors.affiliation: Research and Testing Institute of Military Medicine, S. M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, St. Petersburg, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Nazarov
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia.
authors.forename: E G
authors.initials: EG
authors.lastname: Batotsyrenova
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Lapina
authors.affiliation: Research and Testing Institute of Military Medicine, S. M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, St. Petersburg, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Babkin
authors.affiliation: Research and Testing Institute of Military Medicine, S. M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, St. Petersburg, Russia.
authors.forename: I S
authors.initials: IS
authors.lastname: Berdinskikh
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Melekhova
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia.
authors.forename: K O
authors.initials: KO
authors.lastname: Voitsekhovich
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Lisitskii
authors.affiliation: Institute of Toxicology, Federal Medical-Biological Agency of Russia, St Petersburg, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Kashina
mesh_terms: D000435:Alcoholic Intoxication / Q000378:metabolism; D000818:Animals; D000431:Ethanol / Q000009:adverse effects*; D015870:Gene Expression / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D051381:Rats; D011963:Receptors, GABA-A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C546242:Gabra1 protein, rat; C520011:Gabra2 protein, rat; C546259:Gabra3 protein, rat; C546262:Gabra4 protein, rat; C511535:Gabra5 protein, rat; C546267:Gabrb1 protein, rat; D011963:Receptors, GABA-A; D000431:Ethanol
keywords: GABAA receptors; ethanol; expression; gene