title: Development of antibacterial and high light transmittance bulk materials: Incorporation and sustained release of hydrophobic or hydrophilic antibiotics.
abstract: Infection associated with medical devices is one of the most frequent complications of modern medical biomaterials. Bacteria have a strong ability to attach on solid surfaces, forming colonies and subsequently biofilms. In this work, a novel antibacterial bulk material was prepared through combining poly(dimethyl siloxane) (PDMS) with either hydrophobic or hydrophilic antibiotics (0.1-0.2 wt%). Scanning electron microscopy, water contact angle and UV-vis spectrophotometer were used to measure the changes of surface topography, wettability and optical transmission. For both gentamicin sulfate (GS) and triclosan (TCA), the optical transmission of the PDMS-GS and PDMS-TCA blend films was higher than 90%. Drug release studies showed initial rapid release and later sustained release of GS or TCA under aqueous physiological conditions. The blend films demonstrated excellent bactericidal and sufficient biofilm inhibition functions against Gram-positive bacteria (Staphylococcus aureus, S. aureus) measured by LIVE/DEAD bacterial viability kit staining method. Kirby-Bauer method showed that there was obvious zone of inhibition (7.5-12.5mm). Cytocompatibility assessment against human lens epithelial cells (HLECs) revealed that the PDMS-GS blend films had good cytocompatibility. However, the PDMS-TCA blend films showed certain cytotoxicity against HLECs. The PDMS-0.2 wt% GS blend films were compared to native PDMS in the rabbit subcutaneous S. aureus infection model. The blend films yielded a significantly lower degree of infection than native PDMS at day 7. The achievement of the PDMS-drug bulk materials with high light transmittance, excellent bactericidal function and good cytocompatibility can potentially be widely used as bio-optical materials.
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou 32500, China. Electronic address: wangbailiang2006@aliyun.com.
authors.forename: Bailiang
authors.initials: B
authors.lastname: Wang
authors.affiliation: Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou 32500, China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Binjun
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Yuemei
authors.initials: Y
authors.lastname: Han
authors.affiliation: Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou 32500, China.
authors.forename: Chenghui
authors.initials: C
authors.lastname: Shen
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou 32500, China.
authors.forename: Quankui
authors.initials: Q
authors.lastname: Lin
authors.affiliation: School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou 32500, China. Electronic address: Chenhao823@mail.eye.ac.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D065546:Drug Liberation; D004847:Epithelial Cells / Q000187:drug effects; D005839:Gentamicins / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007908:Lens, Crystalline / Q000166:cytology; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013053:Spectrophotometry; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D013499:Surface Properties; D014260:Triclosan / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D004129:Dimethylpolysiloxanes; D005839:Gentamicins; D008567:Membranes, Artificial; D014260:Triclosan
keywords: Antibacterial; Gentamicin; Light transmittance; Poly(dimethyl siloxane); Sustained release