title: Antioxidant activity of a Lachnum YM226 melanin-iron complex and its influence on cytokine production in mice with iron deficiency anemia.
abstract: The present study aims to investigate the protective effects of an orally administered Lachnum YM226 melanin-iron complex (LM-Fe) against iron deficiency anemia (IDA) in mice. The IDA mouse model was established by feeding mice with iron-deficient food. Different doses of LM-Fe were given to the anaemic mice via intragastric administration, with FeCl3 and FeSO4 used as positive controls. After the iron supplement administration, it was observed that LM-Fe could significantly improve the decreased haemoglobin (Hb) level, and normalize the serum iron (SI) level, total iron-binding capacity (TIBC) and serum ferritin (SF) of the anaemic mice in a dose-dependent manner. In addition, treatment with LM-Fe significantly increased the antioxidant enzyme activities of superoxidase dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in plasma to normal or better. Furthermore, the levels of tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were obviously decreased in the LM-Fe supplemented groups compared with the model group, while the level of interleukin-2 (IL-2) was significantly increased. In conclusion, LM-Fe was efficient at ameliorating the anemia symptoms, improving the activities of antioxidant enzymes and adjusting the immune dysfunction of anaemic mice. Thus, these results demonstrated that LM-Fe might be exploited as an efficient and multifunctional iron supplement.
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. yeming123@sina.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Song
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. yeming123@sina.com.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. yeming123@sina.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Chemical Engineering and Food Processing, Xuancheng Campus, Hefei University of Technology, Xuancheng 242000, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. yeming123@sina.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Shi
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. yeming123@sina.com.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Shaikh
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000032:analysis; D001203:Ascomycota / Q000737:chemistry*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000097:blood; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000008:administration & dosage* / Q000032:analysis; D008297:Male; D008543:Melanins / Q000008:administration & dosage* / Q000032:analysis; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage* / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D053583:Interleukin-1beta; D008543:Melanins; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D005293:Ferritins; D007501:Iron; D002374:Catalase; D005979:Glutathione Peroxidase