title: Cell-free ribosome display and selection of antibodies on arrayed antigens.
abstract: In vitro display technology is a powerful tool for discovery and optimisation of novel antibodies. With increasing demands on various binding molecules in proteomics studies, techniques for a large-scale generation of antibodies or antibody fragments are needed. Here, we describe a novel method for parallel generation of different antibody fragments (scFv) by integrating cell-free ribosome display with array technology. We have demonstrated the procedure by successfully isolating scFv antibodies specific to 16 different cancer biomarkers via a single process. Our results provide proof of principle for multiple production of various scFv antibodies simultaneously.
authors.affiliation: Lab of Recombinant Protein Therapeutics, Chengdu Institute of Biological Products, SinoPharm, Chengdu, Sichuan, P. R. China.
authors.forename: Cong
authors.initials: C
authors.lastname: Cong
authors.affiliation: Lab of Recombinant Protein Therapeutics, Chengdu Institute of Biological Products, SinoPharm, Chengdu, Sichuan, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Lab of Recombinant Protein Therapeutics, Chengdu Institute of Biological Products, SinoPharm, Chengdu, Sichuan, P. R. China.
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: He
authors.affiliation: Lab of Recombinant Protein Therapeutics, Chengdu Institute of Biological Products, SinoPharm, Chengdu, Sichuan, P. R. China.
authors.forename: Yujian
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Lab of Recombinant Protein Therapeutics, Chengdu Institute of Biological Products, SinoPharm, Chengdu, Sichuan, P. R. China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Lab of Recombinant Protein Therapeutics, Chengdu Institute of Biological Products, SinoPharm, Chengdu, Sichuan, P. R. China.
authors.forename: Mingrong
authors.initials: M
authors.lastname: Wang
authors.affiliation: Lab of Recombinant Protein Therapeutics, Chengdu Institute of Biological Products, SinoPharm, Chengdu, Sichuan, P. R. China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000918:Antibody Specificity / Q000276:immunology; D000941:Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D002474:Cell-Free System; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019151:Peptide Library; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding / Q000276:immunology; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D015203:Reproducibility of Results; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000941:Antigens; D014408:Biomarkers, Tumor; D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies
keywords: Cell free ribosome display; Dot blot screening; Multiple antibody generation in vitro; Protein arrays