title: Functional Genomic Analysis of Aspergillus flavus Interacting with Resistant and Susceptible Peanut.
abstract: In the Aspergillus flavus (A. flavus)-peanut pathosystem, development and metabolism of the fungus directly influence aflatoxin contamination. To comprehensively understand the molecular mechanism of A. flavus interaction with peanut, RNA-seq was used for global transcriptome profiling of A. flavus during interaction with resistant and susceptible peanut genotypes. In total, 67.46 Gb of high-quality bases were generated for A. flavus-resistant (af_R) and -susceptible peanut (af_S) at one (T1), three (T2) and seven (T3) days post-inoculation. The uniquely mapped reads to A. flavus reference genome in the libraries of af_R and af_S at T2 and T3 were subjected to further analysis, with more than 72% of all obtained genes expressed in the eight libraries. Comparison of expression levels both af_R vs. af_S and T2 vs. T3 uncovered 1926 differentially expressed genes (DEGs). DEGs associated with mycelial growth, conidial development and aflatoxin biosynthesis were up-regulated in af_S compared with af_R, implying that A. flavus mycelia more easily penetrate and produce much more aflatoxin in susceptible than in resistant peanut. Our results serve as a foundation for understanding the molecular mechanisms of aflatoxin production differences between A. flavus-R and -S peanut, and offer new clues to manage aflatoxin contamination in crops.
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. wanghoumiao@163.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. wanghoumiao@163.com.
authors.forename: Houmiao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. leiyong@caas.cn.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. leiyong@caas.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. yanliying2002@126.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. yanliying2002@126.com.
authors.forename: Liying
authors.initials: L
authors.lastname: Yan
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. wanliyun@caas.cn.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. wanliyun@caas.cn.
authors.forename: Liyun
authors.initials: L
authors.lastname: Wan
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. renxp1972@hotmail.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. renxp1972@hotmail.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. chsl99@163.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. chsl99@163.com.
authors.forename: Silong
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. daixiaofeng@caas.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Dai
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. iewguo@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. peanutlab@oilcrops.cn.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. peanutlab@oilcrops.cn.
authors.forename: Huifang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. liaoboshou@163.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China. liaoboshou@163.com.
authors.forename: Boshou
authors.initials: B
authors.lastname: Liao
mesh_terms: D010367:Arachis / Q000382:microbiology*; D001231:Aspergillus flavus / Q000235:genetics* / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D023281:Genomics; D054884:Host-Pathogen Interactions / Q000235:genetics*; D012331:RNA, Fungal / Q000032:analysis; D012639:Seeds / Q000382:microbiology; D017423:Sequence Analysis, RNA
chemical_list: D012331:RNA, Fungal
keywords: Aspergillus flavus; RNA-seq; aflatoxin biosynthesis; interaction; peanut