title: Loop-mediated isothermal amplification assay for rapid and sensitive detection of sheep pox and goat pox viruses in clinical samples.
abstract: A Loop-mediated isothermal amplification (LAMP) assay targeting the highly conserved DNA polymerase gene of capripox virus genome was developed and evaluated for rapid detection of sheep pox and goat pox viruses. The optimized LAMP assay is found specific and sensitive for amplification of target DNA with a diagnostic sensitivity and specificity of 96.6% and 100% respectively compared to quantitative PCR. The detection rate of LAMP, PCR and Q-PCR assays is found to be 81.5%, 67% and 83% respectively. This LAMP assay has the potential for rapid clinical diagnosis and surveillance of sheep pox and goat pox in field diagnostic laboratories.
authors.affiliation: Pox Virus Disease Laboratory, Division of Virology, ICAR-Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India. Electronic address: gnanamvirol@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Venkatesan
authors.affiliation: Pox Virus Disease Laboratory, Division of Virology, ICAR-Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Yelahanka, Bengaluru 560 064, India.
authors.forename: V
authors.initials: V
authors.lastname: Balamurugan
authors.affiliation: Pox Virus Disease Laboratory, Division of Virology, ICAR-Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India; FMD Laboratory, ICAR-Indan Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India.
authors.forename: V
authors.initials: V
authors.lastname: Bhanuprakash
authors.affiliation: The Director, ICAR-Indian Veterinary Research Institute, Izatnagar 243 122, UP, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Pox Virus Disease Laboratory, Division of Virology, ICAR-Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India.
authors.forename: A B
authors.initials: AB
authors.lastname: Pandey
mesh_terms: D000818:Animals; D018151:Capripoxvirus / Q000235:genetics* / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D015511:Goat Diseases / Q000821:virology*; D006041:Goats / Q000821:virology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011213:Poxviridae Infections / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D012756:Sheep / Q000821:virology; D012757:Sheep Diseases / Q000821:virology*
keywords: Field diagnosis; Goat pox virus; LAMP; Sheep pox virus; Visual detection