title: Interfacial dilatational deformation accelerates particle formation in monoclonal antibody solutions.
abstract: Protein molecules are amphiphilic moieties that spontaneously adsorb at the air/solution (A/S) interface to lower the surface energy. Previous studies have shown that hydrodynamic disruptions to these A/S interfaces can result in the formation of protein aggregates that are of concern to the pharmaceutical industry. Interfacial hydrodynamic stresses encountered by protein therapeutic solutions under typical manufacturing, filling, and shipping conditions will impact protein stability, prompting a need to characterize the contribution of basic fluid kinematics to monoclonal antibody (mAb) destabilization. We demonstrate that dilatational surface deformations are more important to antibody stability when compared to constant-area shear of the A/S interface. We have constructed a dilatational interfacial rheometer that utilizes simultaneous pressure and bubble shape measurements to study the mechanical stability of mAbs under interfacial aging. It has a distinct advantage over methods utilizing the Young-Laplace equation, which incorrectly describes viscoelastic interfaces. We provide visual evidence of particle ejection from dilatated A/S interfaces and spectroscopic data of ejected mAb particles. These rheological studies frame a molecular understanding of the protein-protein interactions at the complex-fluid interface.
authors.affiliation: Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ggf@stanford.edu.
authors.forename: Gigi L
authors.initials: GL
authors.lastname: Lin
authors.forename: Jai A
authors.initials: JA
authors.lastname: Pathak
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Marcia
authors.initials: M
authors.lastname: Carlson
authors.forename: Valeria
authors.initials: V
authors.lastname: Riguero
authors.forename: Yoen Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jean S
authors.initials: JS
authors.lastname: Buff
authors.forename: Gerald G
authors.initials: GG
authors.lastname: Fuller
mesh_terms: D000465:Algorithms; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004548:Elasticity; D057446:Hydrodynamics; D055550:Protein Stability; D012212:Rheology / Q000295:instrumentation; D013501:Surface-Active Agents / Q000737:chemistry; D014783:Viscosity
chemical_list: D000911:Antibodies, Monoclonal; D013501:Surface-Active Agents