title: CDK13, a Kinase Involved in Pre-mRNA Splicing, Is a Component of the Perinucleolar Compartment.
abstract: The perinucleolar compartment (PNC) is a subnuclear stucture forming predominantly in cancer cells; its prevalence positively correlates with metastatic capacity. Although several RNA-binding proteins have been characterized in PNC, the molecular function of this compartment remains unclear. Here we demonstrate that the cyclin-dependent kinase 13 (CDK13) is a newly identified constituent of PNC. CDK13 is a kinase involved in the regulation of gene expression and whose overexpression was found to alter pre-mRNA processing. In this study we show that CDK13 is enriched in PNC and co-localizes all along the cell cycle with the PNC component PTB. In contrast, neither the cyclins K and L, known to associate with CDK13, nor the potential kinase substrates accumulate in PNC. We further show that CDK13 overexpression increases PNC prevalence suggesting that CDK13 may be determinant for PNC formation. This result linked to the finding that CDK13 gene is amplified in different types of cancer indicate that this kinase can contribute to cancer development in human.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, F-66650, Banyuls/Mer, France.
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Even
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, F-66650, Banyuls/Mer, France.
authors.forename: Marie-Line
authors.initials: ML
authors.lastname: Escande
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, F-66650, Banyuls/Mer, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Fayet
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Biologie Intégrative des Organismes Marins (BIOM), Observatoire Océanologique, F-66650, Banyuls/Mer, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Genevière
mesh_terms: D016203:CDC2 Protein Kinase / Q000737:chemistry / Q000378:metabolism*; D002451:Cell Compartmentation; D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D006801:Humans; D008938:Mitosis; D010750:Phosphoproteins / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012322:RNA Precursors / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism; D000097796:Nucleolin
chemical_list: D016203:CDC2 Protein Kinase; D002868:Chromosomal Proteins, Non-Histone; D016213:Cyclins; D010750:Phosphoproteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C047458:fibrillarin; C072262:CDK13 protein, human