title: Sirt1 Regulates p53 Stability and Expression of Its Target S100B during Long-Term Potentiation in Rat Hippocampus.
abstract: Induction of long-term potentiation in rat hippocampus was followed by short-term activation of transcription factor p53 and its subsequent degradation. We studied the effects of EX-527 (inhibitor of deacetylase Sirt1, a negative regulator of p53) and pifi thrin-β (inhibitor of p53-dependent transcription) on the levels of p53 protein and mRNA of its target gene S100B during long-term potentiation. Pifi thrin-β limited the increase in S100B mRNA content after tetanization, which confi rmed signifi cant contribution of p53 in the regulation of S100B during long-term potentiation. EX-527 completely prevented p53 degradation and increased S100B expression induced by tetanization. Thus, Str1 regulates stability of p53 and expression of its target gene S100B in rat hippocampus during long-term potentiation.
authors.affiliation: Design and Technology Institute of Computer Science, Siberian Division of the Russian Academy of Medical Science, Moscow, Russia.
authors.forename: P D
authors.initials: PD
authors.lastname: Lisachev
authors.affiliation: Research Institute of Molecular Biology and Biophysics, Novosibirsk, Russia.|Novosibirsk National Research State University, Novosibirsk, Russia.
authors.forename: V O
authors.initials: VO
authors.lastname: Pustylnyak
authors.affiliation: Research Institute of Molecular Biology and Biophysics, Novosibirsk, Russia. mark@niimbb.ru.
authors.forename: M B
authors.initials: MB
authors.lastname: Shtark
mesh_terms: D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology*; D006624:Hippocampus / Q000378:metabolism*; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit / Q000235:genetics / Q000378:metabolism*; D056564:Sirtuin 1 / Q000378:metabolism*; D014050:Toluene / Q000031:analogs & derivatives* / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: C550547:6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide; D002227:Carbazoles; D006576:Heterocyclic Compounds, 4 or More Rings; D012333:RNA, Messenger; D064568:S100 Calcium Binding Protein beta Subunit; C579158:S100b protein, rat; D016159:Tumor Suppressor Protein p53; C558699:pifithrin-beta; D014050:Toluene; C488368:Sirt1 protein, rat; D056564:Sirtuin 1
keywords: EX-527; Sirt1; long-term potentiation; p53; pifi thrin-β