title: Characterization of Amyloid-β Deposits in Bovine Brains.
abstract: Amyloid-β (Aβ) deposits are seen in aged individuals of many mammalian species that possess the same aminoacid sequence as humans. This study describes Aβ deposition in 102 clinically characterized cattle brains from animals aged 0 to 20 years. Extracellular and intracellular Aβ deposition was detected with 4G8 antibody in the cortex, hippocampus, and cerebellum. X-34 staining failed to stain Aβ deposits, indicating the non β-pleated nature of these deposits. Western blot analysis and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry revealed in Tris, Triton, and formic acid fractions the presence of different Aβ peptides, characterized mainly by C-terminally truncated forms. Exploration of the genetic variability of APOE, PSEN1, and PSEN2 genes involved in Alzheimer's disease pathogenesis revealed several previously unreported polymorphisms. This study demonstrates certain similarities between Aβ deposition patterns exhibited in cattle brains and those in the human brain in early stages of aging. Furthermore, the identification of the same Aβ peptides reported in humans, but unable to form aggregates, supports the hypothesis that cattle may be protected against amyloid plaque formation.
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Vallino Costassa
authors.affiliation: Dipartimento di Scienze Neurologiche Biomediche e del Movimento, Universitá di Verona, Policlinico "G.B. Rossi" Borgo Roma, Verona, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Fiorini
authors.affiliation: Dipartimento di Scienze Neurologiche Biomediche e del Movimento, Universitá di Verona, Policlinico "G.B. Rossi" Borgo Roma, Verona, Italy.
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Zanusso
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Peletto
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Acutis
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Baioni
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Maurella
authors.affiliation: Istituto Neurologico Carlo Besta, Milano, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Tagliavini
authors.affiliation: Istituto Neurologico Carlo Besta, Milano, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Catania
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Gallo
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Monica Lo
authors.initials: ML
authors.lastname: Faro
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Maria Novella
authors.initials: MN
authors.lastname: Chieppa
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Meloni
authors.affiliation: Dipartimento di Scienze Veterinarie, Sezione Clinica Medica, Universitá di Torino, Grugliasco (TO), Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: D'Angelo
authors.affiliation: Dipartimento di Patologia e Sanitá Animale, Universitá di Napoli Federico II, Napoli, Italy.
authors.forename: Orlando
authors.initials: O
authors.lastname: Paciello
authors.affiliation: Laboratorio Marcatori Molecolari, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli Brescia, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Ghidoni
authors.affiliation: Laboratorio Marcatori Molecolari, IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli Brescia, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Tonoli
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Casalone
authors.affiliation: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Torino, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Corona
mesh_terms: D000375:Aging / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D015153:Blotting, Western; D001921:Brain / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D005110:Extracellular Space / Q000378:metabolism; D005787:Gene Frequency; D060005:Genotyping Techniques; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D042541:Intracellular Space / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D011110:Polymorphism, Genetic; D053764:Presenilin-1 / Q000235:genetics; D053766:Presenilin-2 / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D005904:Glial Fibrillary Acidic Protein; D053764:Presenilin-1; D053766:Presenilin-2
keywords: Aging; amyloid beta-protein; cattle; glial cells