title: Mechanism of bile acid-regulated glucose and lipid metabolism in duodenal-jejunal bypass.
abstract: Bile acid plays an important role in regulating blood glucose, lipid and energy metabolism. The present study was implemented to determine the effect of duodenal-jejunal bypass (DJB) on FXR, TGR-5expression in terminal ileum and its bile acid-related mechanism on glucose and lipid metabolism. Immunohistochemistry was used to detect relative gene or protein expression in liver and intestine. Firstly, we found that expression of FXR in liver and terminal ileum of DJB group was significantly higher than that in S-DJB group (P<0.05). In addition, DJB dramatically increased the activation of TGR-5 in the liver of rats. Furthermore, PEPCK, G6Pase, FBPase 1 and GLP-1 were up-regulated by DJB. In conclusion, these results showed that bile acid ameliorated glucose and lipid metabolism through bile acid-FXR and bile acid- TGR-5 signaling pathway.
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute Jinan 250117, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chai
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute Jinan 250117, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute Jinan 250117, China.
authors.forename: Xirui
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute Jinan 250117, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute Jinan 250117, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University Jinan 250012, China.
authors.forename: Sanyuan
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of General Surgery, Shandong Cancer Hospital and Institute Jinan 250117, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Guan
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000601:surgery*; D004195:Disease Models, Animal; D004386:Duodenum / Q000601:surgery*; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D007583:Jejunum / Q000601:surgery*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D001647:Bile Acids and Salts; D006597:Fructose-Bisphosphatase; D052216:Glucagon-Like Peptide 1; D005947:Glucose; D005952:Glucose-6-Phosphatase; D047908:Intracellular Signaling Peptides and Proteins; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D018160:Receptors, Cytoplasmic and Nuclear; D043562:Receptors, G-Protein-Coupled; D000099150:Receptor, Farnesoid X-Activated; C518268:Gpbar1 protein, rat; C582554:Pck1 protein, rat
keywords: Duodenal-jejunal bypass; FXR; TGR-5; bile acid