title: Virgin Olive Oil Enriched with Its Own Phenols or Complemented with Thyme Phenols Improves DNA Protection against Oxidation and Antioxidant Enzyme Activity in Hyperlipidemic Subjects.
abstract: The effects of virgin olive oil (VOO) enriched with its own phenolic compounds (PC) and/or thyme PC on the protection against oxidative DNA damage and antioxidant endogenous enzymatic system (AEES) were estimated in 33 hyperlipidemic subjects after the consumption of VOO, VOO enriched with its own PC (FVOO), or VOO complemented with thyme PC (FVOOT). Compared to pre-intervention, 8-hydroxy-2'-deoxyguanosine (a marker for DNA damage) decreased in the FVOO intervention and to a greater extent in the FVOOT with a parallel significant increase in olive and thyme phenolic metabolites. Superoxide dismutase (AEES enzyme) significantly increased in the FVOO intervention and to a greater extent in the FVOOT with a parallel significant increase in thyme phenolic metabolites. When all three oils were compared, FVOOT appeared to have the greatest effect in protecting against oxidative DNA damage and improving AEES. The sustained intake of a FVOOT improves DNA protection against oxidation and AEES probably due to a greater bioavailability of thyme PC in hyperlipidemic subjects.
authors.affiliation: Pharmacology Unit, Department of Basic Medical Sciences, Facultat de Medicina i Ciències de la Salut, NFOC group, Universitat Rovira i Virgili , C/Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Romeu
authors.affiliation: Food Technology Department, UTPV-XaRTA, Agrotecnio Research Center, Universitat de Lleida , C. Alcalde Rovira Roure 191, 25198 Lleida, Spain.|Unitat de Recerca en Lı́pids i Arteriosclerosis, CIBERDEM, St. Joan de Reus University Hospital, CTNS, IISPV, Facultat de Medicina i Ciències de la Salut, NFOC Group, Universitat Rovira i Virgili , C. Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Rubió
authors.affiliation: Pharmacology Unit, Department of Basic Medical Sciences, Facultat de Medicina i Ciències de la Salut, NFOC group, Universitat Rovira i Virgili , C/Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Sánchez-Martos
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, CIBER de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), IMIM-Institut Hospital del Mar d'Investigacions Mèdiques , C. Doctor Aiguader 88, 08003 Barcelona, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Castañer
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, CIBER de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), IMIM-Institut Hospital del Mar d'Investigacions Mèdiques , C. Doctor Aiguader 88, 08003 Barcelona, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: de la Torre
authors.affiliation: Unitat de Recerca en Lı́pids i Arteriosclerosis, CIBERDEM, St. Joan de Reus University Hospital, CTNS, IISPV, Facultat de Medicina i Ciències de la Salut, NFOC Group, Universitat Rovira i Virgili , C. Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Valls
authors.affiliation: Center for Omics Sciences , Avenida Universitat 1, 43204 Reus, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Ras
authors.affiliation: Unitat de Recerca en Lı́pids i Arteriosclerosis, CIBERDEM, St. Joan de Reus University Hospital, CTNS, IISPV, Facultat de Medicina i Ciències de la Salut, NFOC Group, Universitat Rovira i Virgili , C. Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Pedret
authors.affiliation: Unitat de Recerca en Lı́pids i Arteriosclerosis, CIBERDEM, St. Joan de Reus University Hospital, CTNS, IISPV, Facultat de Medicina i Ciències de la Salut, NFOC Group, Universitat Rovira i Virgili , C. Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Úrsula
authors.initials: Ú
authors.lastname: Catalán
authors.affiliation: Food Technology Department, UTPV-XaRTA, Agrotecnio Research Center, Universitat de Lleida , C. Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: López de las Hazas
authors.affiliation: Food Technology Department, UTPV-XaRTA, Agrotecnio Research Center, Universitat de Lleida , C. Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Motilva
authors.affiliation: Cardiovascular Risk and Nutrition Research Group, CIBER de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), IMIM-Institut Hospital del Mar d'Investigacions Mèdiques , C. Doctor Aiguader 88, 08003 Barcelona, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Fitó
authors.affiliation: Unitat de Recerca en Lı́pids i Arteriosclerosis, CIBERDEM, St. Joan de Reus University Hospital, CTNS, IISPV, Facultat de Medicina i Ciències de la Salut, NFOC Group, Universitat Rovira i Virgili , C. Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Solà
authors.affiliation: Pharmacology Unit, Department of Basic Medical Sciences, Facultat de Medicina i Ciències de la Salut, NFOC group, Universitat Rovira i Virgili , C/Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Giralt
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D018592:Cross-Over Studies; D004247:DNA / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D004311:Double-Blind Method; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005527:Food, Fortified / Q000032:analysis*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000188:drug therapy* / Q000652:urine; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D000069463:Olive Oil / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000008:administration & dosage* / Q000097:blood / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood; D046930:Thymus Plant / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016328:NF-kappa B; D000069463:Olive Oil; D010636:Phenols; D004247:DNA; D013482:Superoxide Dismutase
keywords: enzymatic antioxidants; hyperlipidemia; oxidative stress; phenol enrichment; thyme phenols; virgin olive oil