title: Multicomponent Analysis of the Differential Induction of Secondary Metabolite Profiles in Fungal Endophytes.
abstract: Small molecule histone deacetylase (HDAC) and DNA methyltransferase (DNMT) inhibitors are commonly used to perturb the production of fungal metabolites leading to the induction of the expression of silent biosynthetic pathways. Several reports have described the variable effects observed in natural product profiles in fungi treated with HDAC and DNMT inhibitors, such as enhanced chemical diversity and/or the induction of new molecules previously unknown to be produced by the strain. Fungal endophytes are known to produce a wide variety of secondary metabolites (SMs) involved in their adaptation and survival within higher plants. The plant-microbe interaction may influence the expression of some biosynthetic pathways, otherwise cryptic in these fungi when grown in vitro. The aim of this study was to setup a systematic approach to evaluate and identify the possible effects of HDAC and DNMT inhibitors on the metabolic profiles of wild type fungal endophytes, including the chemical identification and characterization of the most significant SMs induced by these epigenetic modifiers.
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. victor.gonzalez@medinaandalucia.es.
authors.forename: Víctor
authors.initials: V
authors.lastname: González-Menéndez
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. mercedes.perez@medinaandalucia.es.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Pérez-Bonilla
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. ignacio.perez-victoria@medinaandalucia.es.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Pérez-Victoria
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. jesus.martin@medinaandalucia.es.
authors.forename: Jesús
authors.initials: J
authors.lastname: Martín
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. francisca.munoz@medinaandalucia.es.
authors.forename: Francisca
authors.initials: F
authors.lastname: Muñoz
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. Fernando.reyes@medinaandalucia.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Reyes
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. jose.tormo@medinaandalucia.es.
authors.forename: José R
authors.initials: JR
authors.lastname: Tormo
authors.affiliation: Fundación MEDINA, Parque Tecnológico Ciencias de la Salud. Avda. del Conocimiento 34, 18016 Granada, Spain. olga.genilloud@medinaandalucia.es.
authors.forename: Olga
authors.initials: O
authors.lastname: Genilloud
mesh_terms: D053898:Biosynthetic Pathways / Q000187:drug effects; D015257:DNA-Cytosine Methylases / Q000037:antagonists & inhibitors; D060026:Endophytes / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D055432:Metabolomics; D010944:Plants / Q000382:microbiology*; D064210:Secondary Metabolism / Q000187:drug effects*
chemical_list: D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D015257:DNA-Cytosine Methylases
keywords: DNMT; HDAC; epigenetic modifiers; fungal endophytes; metabolomics; secondary metabolites; volcano plots