title: Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells.
abstract: Apical radial glia (aRG), the stem cells in developing neocortex, are unique bipolar epithelial cells, extending an apical process to the ventricle and a basal process to the basal lamina. Here, we report novel features of the Golgi apparatus, a central organelle for cell polarity, in mouse aRGs. The Golgi was confined to the apical process but not associated with apical centrosome(s). In contrast, in aRG-derived, delaminating basal progenitors that lose apical polarity, the Golgi became pericentrosomal. The aRG Golgi underwent evolutionarily conserved, accordion-like compression and extension concomitant with cell cycle-dependent nuclear migration. Importantly, in line with endoplasmic reticulum but not Golgi being present in the aRG basal process, its plasma membrane contained glycans lacking Golgi processing, consistent with direct ER-to-cell surface membrane traffic. Our study reveals hitherto unknown complexity of neural stem cell polarity, differential Golgi contribution to their specific architecture, and fundamental Golgi re-organization upon cell fate change.
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Taverna
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Felipe
authors.initials: F
authors.lastname: Mora-Bermúdez
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Paulina J
authors.initials: PJ
authors.lastname: Strzyz
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Florio
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Icha
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Haffner
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Caren
authors.initials: C
authors.lastname: Norden
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Wilsch-Bräuninger
authors.affiliation: Max-Planck Inst. of Mol. Cell Biol. and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Wieland B
authors.initials: WB
authors.lastname: Huttner
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D015870:Gene Expression; D017930:Genes, Reporter; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008822:Mice, Transgenic; D008938:Mitosis; D058953:Neural Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D011134:Polysaccharides / Q000378:metabolism; D021381:Protein Transport
chemical_list: D011134:Polysaccharides