title: Excretion of Morroniside in Rat Urine After Single Oral and Intravenous Administration.
abstract: This study was designed to develop a sensitive, simple and rapid method for the quantitation of morroniside in rat urine using high-performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) and to investigate the excretion of morroniside in rat urine. The mobile phase consisted of water-acetonitrile (gradient elution) at a flow rate of 0.4 mL/min. Detection was performed using positive-ion electrospray ionization in multiple reaction monitoring (MRM) modes. And the detection of morroniside in rat urine by the LC-MS-MS was accurate and precise from 1.0 to 2,500 ng/mL (a correlation coefficient of 0.9953). The recoveries and matrix effects were all in line with the biological sample measurement requirements. The intraday accuracy was 88.68-105.78% with precision of 6.50-11.19% and the interday accuracy was 95.77-102.43% with precision of 7.08-10.40%. Excretion data of morroniside in rat urine indicated that 21.43‰ (i.g.) and 100.35% (i.v.) of the dose administered was excreted as unconverted form, respectively. And the maximal excretion rate was 27.57 and 482.42 μg/h after oral and intravenous administration, respectively. These results indicated that the developed method has satisfactory sensitivity, accuracy and precision for the quantification of morroniside in rat urine.
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Ji'nan 250062, China Key Laboratory of Drug Metabolism and Pharmacokinetics, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Jinglai
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Drug Metabolism and Pharmacokinetics, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China zhangzhenqingpharm@163.com.
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000652:urine*; D051381:Rats; D053719:Tandem Mass Spectrometry; D016482:Urinalysis / Q000379:methods*
chemical_list: D006027:Glycosides; C488401:morroniside