title: Investigation of in vitro cytotoxic, mutagenic and anti-mutagenic effects of shirazolide extracted from Jurinea leptoloba.
abstract: Shirazolide is an elemanolide isolated from Jurinea leptoloba DC. The aim of this study was to determine the cytotoxic, mutagenic and anti-mutagenic properties of shirazolide from J. leptoloba DC in vitro. Cytotoxicity was measured using a modified MTT (3-(4,5-di methyl thiazol-2-yl)-2,5-di phenyltetrazolium bromide) assay on normal human lymphocytes and tumor HeLa cells, showing that the cytotoxicity of shirazolide is much higher for HeLa cells than for normal lymphocytes. Mutagenic and anti-mutagenic activities of shirazolide were evaluated using the Salmonella typhimurium tester strains TA98 and TA100 showing anti-mutagenic properties against the former strain under metabolic activation.
authors.affiliation: a Department of Chemistry, College of Science, Takestan Branch , Islamic Azad University , Takestan , Iran.
authors.forename: Mahboubeh
authors.initials: M
authors.lastname: Taherkhani
authors.affiliation: b Department of Chemistry, College of Science, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Abdolhossein
authors.initials: A
authors.lastname: Rustaiyan
keywords: Shirazolide; anti-mutagenic; cytotoxicity