title: Influence of carbon nanomaterial defects on the formation of protein corona.
abstract: In any physiological media, carbon nanomaterials (CNM) strongly interact with biomolecules leading to the formation of biocorona, which subsequently dictate the physiological response and the fate of CNMs. Defects in CNMs play an important role not only in material properties but also in the determination of how materials interact at the nano-bio interface. In this article, we probed the influence of defect-induced hydrophilicity on the biocorona formation using micro-Raman, photoluminescence, infrared spectroscopy, electrochemistry, and molecular dynamics simulations. Our results show that the interaction of proteins (albumin and fibrinogen) with CNMs is strongly influenced by charge-transfer between them, inducing protein unfolding which enhances conformational entropy and higher protein adsorption.
authors.affiliation: Department of Physics and Astronomy, Clemson Nanomaterials Center, Clemson University, Clemson, South Carolina 29634, United States ; Laboratory of nano-biophysics, Clemson University, Clemson, South Carolina 29634, United States.
authors.forename: Bishwambhar
authors.initials: B
authors.lastname: Sengupta
authors.affiliation: Department of Physics and Astronomy, Clemson Nanomaterials Center, Clemson University, Clemson, South Carolina 29634, United States ; Laboratory of nano-biophysics, Clemson University, Clemson, South Carolina 29634, United States.
authors.forename: Wren E
authors.initials: WE
authors.lastname: Gregory
authors.affiliation: Department of Physics and Astronomy, Clemson Nanomaterials Center, Clemson University, Clemson, South Carolina 29634, United States.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, South Carolina 29634, United States.
authors.forename: Siva
authors.initials: S
authors.lastname: Dasetty
authors.affiliation: Department of Physics and Astronomy, Clemson Nanomaterials Center, Clemson University, Clemson, South Carolina 29634, United States.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Karakaya
authors.affiliation: Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, The University of Colorado Anschutz Medical Campus, Aurora, CO 80045, United States.
authors.forename: Jared M
authors.initials: JM
authors.lastname: Brown
authors.affiliation: Department of Physics and Astronomy, Clemson Nanomaterials Center, Clemson University, Clemson, South Carolina 29634, United States ; COMSET, Clemson University, Anderson, SC 29625, United States.
authors.forename: Apparao M
authors.initials: AM
authors.lastname: Rao
authors.affiliation: Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina 29634, United States.
authors.forename: John K
authors.initials: JK
authors.lastname: Barrows
authors.affiliation: Laboratory of nano-biophysics, Clemson University, Clemson, South Carolina 29634, United States.
authors.forename: Sapna
authors.initials: S
authors.lastname: Sarupria
authors.affiliation: Department of Physics and Astronomy, Clemson Nanomaterials Center, Clemson University, Clemson, South Carolina 29634, United States ; Laboratory of nano-biophysics, Clemson University, Clemson, South Carolina 29634, United States. ; COMSET, Clemson University, Anderson, SC 29625, United States.
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Podila