title: HOXA10, EMX2 and TENM1 expression in the mid-secretory endometrium of infertile women with a Müllerian duct anomaly.
abstract: Homeobox A10 (HOXA10) and empty spiracles homeobox 2 (EMX2) are two transcription factors necessary for female Müllerian duct differentiation and development. They are thought to play important roles in embryo implantation in mice and humans. The EMX2 gene is a known direct target of HOXA10 in the reproductive tract. Human TENM1 is directly regulated by EMX2 and is expressed during embryonic pattern formation and morphogenesis. This study aimed to investigate expression patterns of HOXA10, EMX2 and TENM1 in the mid-secretory endometrium of infertile patients with a Müllerian duct anomaly causing a partially septate uterus. Thirteen mid-secretory endometrial tissue samples were collected from women with partially septate uteri and 12 from women with normal uteri as controls. Expression levels of HOXA10, EMX2 and TENM1 mRNA and protein in the mid-secretory endometrium of infertile patients and controls were measured by quantitative reverse transcription polymerase chain reaction and western blotting. Compared with controls, mRNA and protein expression levels of HOXA10 decreased significantly (P < 0.01), whereas EMX2 and TENM1 increased dramatically in patients with Müllerian duct anomaly (P < 0.001). Changes in HOXA10, EMX2, and TENM1 expression levels might act in infertile women with Müllerian duct anomaly to cause a partially septate uterus.
authors.affiliation: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Anhui Engineering Research Center for Low Temperature Medical Science and Artificial Organs, Hefei, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Minna
authors.initials: M
authors.lastname: Luo
authors.affiliation: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Anhui Engineering Research Center for Low Temperature Medical Science and Artificial Organs, Hefei, China.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Anhui Engineering Research Center for Low Temperature Medical Science and Artificial Organs, Hefei, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Anhui Engineering Research Center for Low Temperature Medical Science and Artificial Organs, Hefei, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Anhui Engineering Research Center for Low Temperature Medical Science and Artificial Organs, Hefei, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xing
authors.affiliation: National Research Institute for Family Planning, Beijing 100081, China; Graduate School of Peking Union Medical College, Beijing, China. Electronic address: wbbahu@163.com.
authors.forename: Binbin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Center for Reproductive Medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China; Anhui Engineering Research Center for Low Temperature Medical Science and Artificial Organs, Hefei, China. Electronic address: caoyunxia6@126.com.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Cao
mesh_terms: D015153:Blotting, Western; D005260:Female; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007246:Infertility / Q000235:genetics / Q000378:metabolism*; D008183:Luteal Phase; D009095:Mullerian Ducts / Q000002:abnormalities* / Q000196:embryology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019063:Tenascin / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000099260:Empty Spiracles Homeobox Proteins
chemical_list: D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D019063:Tenascin; D014157:Transcription Factors; D000099260:Empty Spiracles Homeobox Proteins; C120530:teneurin-1; C069634:HOXA10 protein, human
keywords: EMX2; HOXA10; Müllerian duct anomaly; TENM1; infertility; partially septate uterus