title: Agromyces insulae sp. nov., an actinobacterium isolated from a soil sample.
abstract: A novel Gram-reaction-positive, non-motile, aerobic bacterium, designated CFH S0483T, was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. 16S rRNA gene sequence analysis showed that the strain is a member of the genus Agromyces and has highest 16S rRNA gene sequence similarities with Agromyces humatus DSM 16389T (97.3 %) and Agromyces ramosus DSM 43045T (97.1 %), and similarities  < 97.0 % with type strains of other species of the genus Agromyces. Strain CFH S0483T was able to grow at 10-37 °C, at pH 7.0-9.0 and tolerated NaCl up to 2.0 % (w/v). The whole-cell sugars were mannose, galactose, glucose and ribose. The isolate contained l-2,4-diaminobutyric acid, d-alanine, d-glutamic acid and glycine in the cell-wall peptidoglycan. Strain CFH S0483T exhibited a menaquinone system with MK-12, and the major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The genomic DNA G+C content of strain CFH S0483T was 71.6 mol%. Based on the phylogenetic and phenotypic analysis, and low DNA-DNA hybridization values, strain CFH S0483T could not be classified into any recognized species of the genus Agromyces. Strain CFH S0483T is therefore considered to represent a novel species of the genus Agromyces, for which the name Agromyces insulae sp. nov. is proposed. The type strain is CFH S0483T ( = KCTC 39117T = CCTCC AB 2014301T).
authors.affiliation: College of Fisheries, Henan Normal University,Xinxiang, 453007, PRChina.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Huang
authors.affiliation: College of Life Sciences and Technology, Xinxiang Medical University,Xinxiang, 453003, PRChina.|Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091, PRChina.
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.affiliation: College of Fisheries, Henan Normal University,Xinxiang, 453007, PRChina.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Fisheries, Henan Normal University,Xinxiang, 453007, PRChina.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Meng
authors.affiliation: College of Fisheries, Henan Normal University,Xinxiang, 453007, PRChina.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091, PRChina.|Department of Food Technology, School of Biotechnology and Food Technology, Hanoi University of Science and Technology,Hanoi,Vietnam.
authors.forename: Thi-Nhan
authors.initials: TN
authors.lastname: Khieu
authors.affiliation: College of information and Management, Guangxi Medical University,Nanning, 530021, PRChina.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University,Kunming, 650091, PRChina.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275, PRChina.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: College of Fisheries, Henan Normal University,Xinxiang, 453007, PRChina.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Nie
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000613:Aminobutyrates / Q000737:chemistry; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014744:Vietnam; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D000613:Aminobutyrates; D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C005959:2,4-diaminobutyric acid