title: On origin and evolution of carbonic anhydrase isozymes: A phylogenetic analysis from whole-enzyme to active site.
abstract: Genetic evolution of carbonic anhydrase enzyme provides an interesting instance of functional similarity in spite of structural diversity of the members of a given family of enzymes. Phylogenetic analysis of α-, β- and γ-carbonic anhydrase was carried out to determine the evolutionary relationships among various members of the family with the enzyme marking its presence in a wide range of cellular and chromosomal locations. The presence of more than one class of enzymes in a particular organism was revealed by phylogenetic time tree. The evolutionary relationships among the members of animal, plant and microbial kingdom were developed. The study revises a long-established notion of kingdom-specificity of the different classes of carbonic anhydrases and provides a new version of the presence of multiple classes of carbonic anhydrases in a single organism and the presence of a given class of carbonic anhydrase across different kingdoms.
authors.affiliation: Quantum and Molecular Engineering Laboratory, Department of Chemical Engineering, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Srijoni
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Quantum and Molecular Engineering Laboratory, Department of Chemical Engineering, Indian Institute of Technology Kharagpur, Kharagpur 721302, India; Centre for Theoretical Studies, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. Electronic address: parag@che.iitkgp.ernet.in.
authors.forename: Parag A
authors.initials: PA
authors.lastname: Deshpande
mesh_terms: D002256:Carbonic Anhydrases / Q000737:chemistry*; D020134:Catalytic Domain; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry*; D010802:Phylogeny
chemical_list: D007527:Isoenzymes; D002256:Carbonic Anhydrases
keywords: Active site; Biological function; Carbonic anhydrases; Kingdom-specificity; Sequence alignment; Structural similarity