title: Ovarian kisspeptin expression is related to age and to monocyte chemoattractant protein-1.
abstract: OBJECTIVE
  The objective of this study was to test the hypothesis that ovarian kisspeptin (kiss1) and its receptor (kiss1r) expression are affected by age, obesity, and the age- and obesity-related chemokine monocyte chemoattractant protein-1 (MCP-1).
  
  
  METHODS
  Ovaries from reproductive-aged and older C57BL/6J mice fed normal chow (NC) or high-fat (HF) diet, ovaries from age-matched young MCP-1 knockout and young control mice on NC, and finally, cumulus and mural granulosa cells (GCs) from women who underwent in vitro fertilization (IVF) were collected. Kiss1, kiss1r, anti-Mullerian hormone (AMH), and AMH receptor (AMHR-II) messenger RNA (mRNA) expression levels were quantified using real-time polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  In mouse ovaries, kiss1 and kiss1r mRNA levels were significantly higher in old compared to reproductive-aged mice, and diet-induced obesity did not alter kiss1 or kiss1r mRNA levels. Compared to young control mice, young MCP-1 knockout mice had significantly lower ovarian kiss1 mRNA but significantly higher AMH and AMHR-II mRNA levels. In human cumulus GCs, kiss1r mRNA levels were positively correlated with age but not with BMI. There was no expression of kiss1 mRNA in either cumulus or mural GCs.
  
  
  CONCLUSIONS
  These data suggest a possible age-related physiologic role for the kisspeptinergic system in ovarian physiology. Additionally, the inflammatory MCP-1 may be associated with kiss1 and AMH genes, which are important in ovulation and folliculogenesis, respectively.
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproductive Biology, New York University School of Medicine, 180 Varick Street, sixth floor, New York, NY, 10014, USA. zom00@hotmail.com.
authors.forename: Zaher
authors.initials: Z
authors.lastname: Merhi
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, USA.
authors.forename: Kimberley
authors.initials: K
authors.lastname: Thornton
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, College of Medicine, University of Vermont, Burlington, VT, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Bonney
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, College of Medicine, University of Vermont, Burlington, VT, USA.|Department of Neurological Sciences, College of Medicine, University of Vermont, Burlington, VT, USA.
authors.forename: Marilyn J
authors.initials: MJ
authors.lastname: Cipolla
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, USA.|Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, USA.|Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, USA.
authors.forename: Maureen J
authors.initials: MJ
authors.lastname: Charron
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, USA.
authors.forename: Erkan
authors.initials: E
authors.lastname: Buyuk
mesh_terms: D000375:Aging / Q000235:genetics* / Q000473:pathology; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000096:biosynthesis / Q000235:genetics; D018932:Chemokine CCL2 / Q000235:genetics*; D059305:Diet, High-Fat; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D059648:Kisspeptins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000235:genetics* / Q000473:pathology; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics; D000074502:Receptors, Kisspeptin-1; D018000:Receptors, Peptide / Q000096:biosynthesis / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; C106628:KISS1 protein, human; C118688:KISS1R protein, human; D059648:Kisspeptins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D000074502:Receptors, Kisspeptin-1; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; C084621:anti-Mullerian hormone receptor; D054304:Anti-Mullerian Hormone
keywords: Aging; Kisspeptin; MCP-1; Obesity; Ovary