title: H2O2-triggered bubble generating antioxidant polymeric nanoparticles as ischemia/reperfusion targeted nanotheranostics.
abstract: Overproduction of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) leads to oxidative stress, causing inflammation and cellular damages and death. H2O2 is one of the most stable and abundant ROS and H2O2-mediated oxidative stress is considered as a key mediator of cellular and tissue damages during ischemia/reperfusion (I/R) injury. Therefore, H2O2 could hold tremendous potential as a diagnostic biomarker and therapeutic target for oxidative stress-associated inflammatory conditions such as I/R injury. Here, we report a novel nanotheranostic agent that can express ultrasound imaging and simultaneous therapeutic effects for hepatic I/R treatment, which is based on H2O2-triggered CO2-generating antioxidant poly(vanillin oxalate) (PVO). PVO nanoparticles generate CO2 through H2O2-triggered oxidation of peroxalate esters and release vanillin, which exerts antioxidant and anti-inflammatory activities. PVO nanoparticles intravenously administrated remarkably enhanced the ultrasound signal in the site of hepatic I/R injury and also effectively suppressed the liver damages by inhibiting inflammation and apoptosis. To our best understanding, H2O2-responsive PVO is the first platform which generates bubbles to serve as ultrasound contrast agents and also exerts therapeutic activities. We therefore anticipate that H2O2-triggered bubble-generating antioxidant PVO nanoparticles have great potential for ultrasound imaging and therapy of H2O2-associated diseases.
authors.affiliation: Department of BIN Convergence Technology, Chonbuk National University, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea.
authors.forename: Changsun
authors.initials: C
authors.lastname: Kang
authors.affiliation: Department of BIN Convergence Technology, Chonbuk National University, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea.
authors.forename: Wooram
authors.initials: W
authors.lastname: Cho
authors.affiliation: Department of BIN Convergence Technology, Chonbuk National University, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea.
authors.forename: Minhyung
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of BIN Convergence Technology, Chonbuk National University, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea.
authors.forename: Jinsub
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Electronics Engineering, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Electronics Engineering, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Shin
authors.affiliation: Department of Electronics Engineering, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea.
authors.forename: Chulgyu
authors.initials: C
authors.lastname: Song
authors.affiliation: Department of BIN Convergence Technology, Chonbuk National University, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea; Department of Polymer⋅Nano Science and Technology, Chonbuk National University, Baekjedaero 567, Jeonju, Chonbuk 561-756, Republic of Korea. Electronic address: dlee@chonbuk.ac.kr.
authors.forename: Dongwon
authors.initials: D
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D004146:Dioxanes / Q000737:chemistry / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000188:drug therapy*; D000068936:Theranostic Nanomedicine; D014463:Ultrasonography
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004146:Dioxanes; D011091:Polyesters; D011108:Polymers; D017382:Reactive Oxygen Species; C584945:poly(vanillin oxalate); D006861:Hydrogen Peroxide
keywords: Hydrogen peroxide; Ischemia/reperfusion; Polymer; Theranostic; Ultrasound imaging