title: Effect of Leptin Administration on Circulating Apolipoprotein CIII levels in Patients With Lipodystrophy.
abstract: Apolipoprotein CIII (apoCIII), an inhibitor of lipoprotein lipase, plays an important role in triglyceride metabolism. However, the role of apoCIII in hypertriglyceridemia in lipodystrophy and the effects of leptin replacement on apoCIII levels are unknown.
  
  
  
  The objective of the study was to test the hypotheses that apoCIII is elevated in hypertriglyceridemic patients with lipodystrophy and that leptin replacement in these patients lowers circulating apoCIII.
  
  
  
  Using a post hoc cross-sectional case-control design, we compared serum apoCIII levels from patients with lipodystrophy not associated with HIV (n = 60) and age-, gender-, race-, and ethnicity-matched controls (n = 54) participating in ongoing studies at the National Institutes of Health. In a prospective, open-label, ongoing study, we studied the effects of 6–12 months of leptin replacement on apoCIII in lipodystrophy patients as an exploratory outcome.
  
  
  
  ApoCIII was higher in lipodystrophy patients (geometric mean [25th and 75th percentiles]) (23.9 mg/dL [14.6, 40.3]) compared with controls (14.9 mg/dL [12.3, 17.7]) (P < .0001). ApoCIII and triglyceride levels were positively correlated in patients with lipodystrophy (R = 0.72, P < .0001) and healthy controls (R = 0.6, P < .0001). Leptin replacement (6–12 mo) did not significantly alter apoCIII (before leptin: 23.4 mg/dL [14.5, 40.1]; after leptin: 21.4 mg/dL [16.7, 28.3]; P = .34).
  
  
  
  Leptin replacement in lipodystrophy did not alter serum apoCIII levels. Elevated apoCIII may play a role in the hypertriglyceridemia of lipodystrophy independent of leptin deficiency and replacement.
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Andrea
authors.initials: A
authors.lastname: Kassai
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Ranganath
authors.initials: R
authors.lastname: Muniyappa
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Levenson
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Mary F
authors.initials: MF
authors.lastname: Walter
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Brent S
authors.initials: BS
authors.lastname: Abel
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Michael
authors.initials: M
authors.lastname: Ring
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Simeon I
authors.initials: SI
authors.lastname: Taylor
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Sudha B
authors.initials: SB
authors.lastname: Biddinger
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Monica C
authors.initials: MC
authors.lastname: Skarulis
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Phillip
authors.initials: P
authors.lastname: Gorden
authors.affiliation: Diabetes, Endocrinology and Obesity Branch (A.K., R.M., B.S.A., M.R., M.C.S., P.G., R.J.B.), Clinical Core Laboratory (M.F.W.), National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; Division of Endocrinology (A.E.L., S.B.B.), Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115; and Division of Endocrinology, Diabetes, and Nutrition (S.I.T.), University of Maryland School of Medicine, Baltimore, Maryland 21201.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Brown
mesh_terms: D000328:Adult; D000818:Animals; D053305:Apolipoprotein C-III / Q000097:blood*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D020738:Leptin / Q000494:pharmacology*; D008060:Lipodystrophy / Q000097:blood*; D008297:Male; D051379:Mice; D008875:Middle Aged; D055815:Young Adult
chemical_list: D053305:Apolipoprotein C-III; D020738:Leptin