title: RNA fragments mimicking tRNA analogs interact with cytochrome c.
abstract: In times, when drug seeking assays focus on the natural molecular triggers and their analogs, a deeper insight into molecular mechanisms governing the initial step of intrinsic apoptosis (cytochrome c release) is essential to suppress the immortality of pathologically changed cells. In this study, we examined RNA molecules mimicking mitochondrial tRNAs interacting with cytochrome c and possibly affecting its cellular function. tRNA analogs were designed and synthesized prior to the conformational analysis and gel assays clearly stating the nucleic acid-protein complex formation. The circular dichroism spectroscopic (CD) and microscale thermophoresis examination revealed the structural and conformational differences between four tRNA analogs in their interactions with cytochrome c. Obtained CD spectra and gel studies resulted in the complex ratio estimation and conclusion that not only the complex formation may be preferential towards specific tRNAs present in the cell, but nucleobase modifications are not essential for such interaction.
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90363, Lodz, Poland.
authors.forename: Roza
authors.initials: R
authors.lastname: Pawlowska
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90363, Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Janicka
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90363, Lodz, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Jedrzejczyk
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90363, Lodz, Poland. achworos@cbmm.lodz.pl.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Chworos
mesh_terms: D002942:Circular Dichroism; D045304:Cytochromes c / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D012343:RNA, Transfer / Q000138:chemical synthesis / Q000378:metabolism*
chemical_list: D045304:Cytochromes c; D012343:RNA, Transfer
keywords: Apoptosis; Circular dichroism; Cytochrome c; Microscale thermophoresis; tRNA analogs; tRNA structure