title: Genetic Analysis of IL-17 Gene Polymorphisms in Gout in a Male Chinese Han Population.
abstract: Interleukin (IL)-17 is a proinflammatory cytokine mainly secreted by activated T helper 17 cells and involved in inflammatory immune responses. This study aimed to investigate the association between IL-17 variants as well as serum IL-17 levels with gout in male Chinese Han individuals. A total of 1,101 male gout patients and 1,239 ethic-matched controls were enrolled. Genetic distributions of three variants (rs2275913 in IL-17A, rs763780 in IL-17F, and rs4819554 in IL-17RA) were detected by real-time polymerase chain reaction using the Taqman probe method. The plasma concentrations of IL-17A and IL-17F were measured in 228 gout patients and 198 controls that came from above samples by an enzyme-linked immunosorbent assay. No significant differences were observed in the genetic distribution of these polymorphisms between cases and controls (rs2275913: χ2 = 0.15, p = 0.928 by genotype, χ2 = 0.14, p = 0.711 by allele; rs763780: χ2 = 2.24, p = 0.326 by genotype, χ2 = 0.26, p = 0.609 by allele; rs4819554: χ2 = 1.79, p = 0.409 by genotype, χ2 = 1.46, p = 0.227 by allele). Levels of serum IL-17A and IL-17F were significantly decreased in gout patients (both p<0.001). However, no difference was observed in acute gout patients between different genotypic carriers of rs2275913 and rs763780 regarding serum IL-17A and IL-17F levels (p>0.05). Although the genetic variants in IL-17 we studied in this research do not appear to be involved in the development of gout in male Chinese Han individuals, the IL-17 cytokine family may participate in gouty inflammation in an undefined way, which requires further validation.
authors.affiliation: Shandong Provincial Key Laboratory of Metabolic Disease, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Shandong Provincial Key Laboratory of Metabolic Disease, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Xinde
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Rheumatology, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Prenatal Diagnosis Center, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Guo
authors.affiliation: Prenatal Diagnosis Center, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Liu
authors.affiliation: Shandong Provincial Key Laboratory of Metabolic Disease, the Affiliated Hospital of Qingdao University, Qingdao, China.
authors.forename: Changgui
authors.initials: C
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D015415:Biomarkers; D016022:Case-Control Studies; D002681:China; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006073:Gout / Q000097:blood / Q000235:genetics*; D006801:Humans; D020381:Interleukin-17 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D015415:Biomarkers; D020381:Interleukin-17