title: Study on the potential application of salivary inorganic anions in clinical diagnosis by capillary electrophoresis coupled with contactless conductivity detection.
abstract: A capillary electrophoresis approach with capacitively coupled contactless conductivity detection method has been developed for the determination of inorganic metabolites (thiocyanate, nitrite and nitrate) in human saliva. Field amplified sample injection, as a simple sample stacking technique, was used in conjunction for online preconcentration of above inorganic anions. A selective separation for the target anions from other coexisting constituents present in saliva could be obtained within 14min in a 10mmol/L His-90mmol/L HAc buffer (pH 3.70) at the separation voltage of -18kV. The limits of detection and limits of quantification of the three analytes were within the range of 3.1-4.9ng/mL (S/N=3) and 10-16ng/mL (S/N=10), respectively. The average recovery data were in the range of 81-108% at three different concentrations. This method provides a simple, rapid and direct approach for metabolite analyses of nitric oxide and cyanide based on noninvasive saliva sample, which presents a potential fast screening tool for clinical test.
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Yiliang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
authors.forename: Jiannong
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China. Electronic address: qcchu@chem.ecnu.edu.cn.
authors.forename: Qingcui
authors.initials: Q
authors.lastname: Chu
authors.affiliation: Shanghai Putuo District People's Hospital, Shanghai 200060, China. Electronic address: dongping-huang@163.com.
authors.forename: Dongping
authors.initials: D
authors.lastname: Huang
mesh_terms: D000328:Adult; D000838:Anions / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000191:economics / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D009566:Nitrates / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D013861:Thiocyanates / Q000032:analysis*
chemical_list: D000838:Anions; D009566:Nitrates; D009573:Nitrites; D013861:Thiocyanates; C031760:thiocyanate
keywords: Capacitively coupled contactless conductivity detection; Capillary electrophoresis; Clinical analysis; Field amplified sample injection; Inorganic anions; Saliva