title: Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea.
abstract: In this study, the anti-adipogenetic activity of 300 plant extracts was investigated using an Oil Red O staining assay in a 3T3-L1 cell line. Our results indicate that three plants, including the stem and leaf of Physalis angulata, the whole grass of Solidago virgaurea, and the root of Dioscorea nipponica, produced over 90% inhibition of adipogenesis. Kaempferol-3-O-rutinoside, which demonstrated a 48.2% inhibitory effect on adipogenesis without cytotoxicity, was isolated from the butanol layer of a water extract of S. virgaurea guided by the anti-adipogenesis assay in 3T3-L1. PPAR-γ and C/EBPα expression levels were determined using western blot, and our results indicate that kaempferol-3-O-rutinoside has a strong anti-adipogenic effect in 3T3-L1 cells through the suppression of increases in PPAR-γ and C/EBPα expression.
authors.affiliation: Department of Food Science and Nutrition, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, Korea. ggaeby77@gmail.com.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Jang
authors.affiliation: Department of Food Science and Nutrition, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, Korea. wangwq01234@gmail.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Boichemistry, Hallym University Medical School, Okchon-dong, Chuncheon, Gangwon-do 200-702, Korea. sindelela99@nate.com.
authors.forename: Jeong-Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Boichemistry, Hallym University Medical School, Okchon-dong, Chuncheon, Gangwon-do 200-702, Korea. jyolee@hallym.ac.kr.|Institute of Natural Medicine, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, Korea. jyolee@hallym.ac.kr.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, Korea. limss@hallym.ac.kr.|Institute of Natural Medicine, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, Korea. limss@hallym.ac.kr.
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D001688:Biological Products / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D027742:Dioscorea / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D044949:Kaempferols / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D032321:Physalis / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D036624:Solidago / Q000737:chemistry*
chemical_list: D001688:Biological Products; D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D044949:Kaempferols; D047495:PPAR gamma; D010936:Plant Extracts; C492687:kaempferol-3-O-rutinoside
keywords: Solidago virgaurea; kaempferol-3-O-rutinoside; obesity; screening