title: Effect of Endothelial Cells on Angiogenic Properties of Multipotent Stromal Cells from the Umbilical Cord during Angiogenesis Modeling in the Basement Membrane Matrix.
abstract: Short-term cell culturing on basement membrane matrix is a common and very convenient in vitro model of angiogenesis. We studied the possibility of interaction of multipotent stromal cells from the umbilical cord and Ea.hy926 endothelial cells on this model at the early and late periods of the experiment. Multipotent stromal cells alone and in combination with endothelial cells formed an unstable tubular network. Clusters formed after its disassembling later became the sprouting centers in co-culture of the two cell types, but not in pure culture of multipotent stromal cells. Multipotent stromal cells with CD31+ phenotype constitute the structural basis of newly formed stable 3D capillary-like network. Prolongation of the time of culturing and combination of the two in vitro models of angiogenesis (tubulogenesis and sprouting) allowed more complete assessment of the angiogenic potential of multipotent stromal cells.
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.|Research Institute of Human Morphology, Moscow, Russia.
authors.forename: I V
authors.initials: IV
authors.lastname: Arutyunyan
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. fatkhudinov@gmail.com.|Research Institute of Human Morphology, Moscow, Russia. fatkhudinov@gmail.com.|N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia. fatkhudinov@gmail.com.
authors.forename: T H
authors.initials: TH
authors.lastname: Fatkhudinov
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.|Research Institute of Human Morphology, Moscow, Russia.|N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: El'chaninov
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.|Research Institute of Human Morphology, Moscow, Russia.|N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Makarov
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.|Research Institute of Human Morphology, Moscow, Russia.
authors.forename: E Yu
authors.initials: EY
authors.lastname: Kananykhina
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.|Research Institute of Human Morphology, Moscow, Russia.|N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N Yu
authors.initials: NY
authors.lastname: Usman
authors.affiliation: N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E Sh
authors.initials: ESh
authors.lastname: Raimova
authors.affiliation: Medical Genetic Research Center, Moscow, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Goldshtein
authors.affiliation: Research Institute of Human Morphology, Moscow, Russia.
authors.forename: G B
authors.initials: GB
authors.lastname: Bol'shakova
mesh_terms: D001485:Basement Membrane / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Matrigel; basement membrane matrix; endothelial cells; in vitro angiogenesis; multipotent stromal cells of umbilical cord