title: Diammonium phosphate stimulates transcription of L-lactate dehydrogenase leading to increased L-lactate production in the thermotolerant Bacillus coagulans strain.
abstract: Exploration of cost-effective fermentation substrates for efficient lactate production is an important economic objective. Although some organic nitrogen sources are also cheaper, inorganic nitrogen salts for lactate fermentation have additional advantages in facilitating downstream procedures and significantly improving the commercial competitiveness of lactate production. In this study, we first established an application of diammonium phosphate to replace yeast extract with a reduced 90 % nitrogen cost for a thermotolerant Bacillus coagulans strain. In vivo enzymatic and transcriptional analyses demonstrated that diammonium phosphate stimulates the gene expression of L-lactate dehydrogenase, thus providing higher specific enzyme activity in vivo and increasing L-lactic acid production. This new information provides a foundation for establishing a cost-effective process for polymer-grade L-lactic acid production in an industrial setting.
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.|School of Food Engineering and Biological Technology, Tianjin University of Science & Technology, Tianjin, 300457, China.
authors.forename: Lifan
authors.initials: L
authors.lastname: Sun
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Food Engineering and Biological Technology, Tianjin University of Science & Technology, Tianjin, 300457, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China. yub@im.ac.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D000070000:Bacillus coagulans / Q000201:enzymology / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D015870:Gene Expression / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010710:Phosphates; C024788:ammonium phosphate; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase
keywords: Bacillus coagulans; Diammonium phosphate; L-Lactate; L-Lactate dehydrogenase; Thermotolerant