title: Multivariate Analysis of Genotype-Phenotype Association.
abstract: With the advent of modern imaging and measurement technology, complex phenotypes are increasingly represented by large numbers of measurements, which may not bear biological meaning one by one. For such multivariate phenotypes, studying the pairwise associations between all measurements and all alleles is highly inefficient and prevents insight into the genetic pattern underlying the observed phenotypes. We present a new method for identifying patterns of allelic variation (genetic latent variables) that are maximally associated-in terms of effect size-with patterns of phenotypic variation (phenotypic latent variables). This multivariate genotype-phenotype mapping (MGP) separates phenotypic features under strong genetic control from less genetically determined features and thus permits an analysis of the multivariate structure of genotype-phenotype association, including its dimensionality and the clustering of genetic and phenotypic variables within this association. Different variants of MGP maximize different measures of genotype-phenotype association: genetic effect, genetic variance, or heritability. In an application to a mouse sample, scored for 353 SNPs and 11 phenotypic traits, the first dimension of genetic and phenotypic latent variables accounted for >70% of genetic variation present in all 11 measurements; 43% of variation in this phenotypic pattern was explained by the corresponding genetic latent variable. The first three dimensions together sufficed to account for almost 90% of genetic variation in the measurements and for all the interpretable genotype-phenotype association. Each dimension can be tested as a whole against the hypothesis of no association, thereby reducing the number of statistical tests from 7766 to 3-the maximal number of meaningful independent tests. Important alleles can be selected based on their effect size (additive or nonadditive effect on the phenotypic latent variable). This low dimensionality of the genotype-phenotype map has important consequences for gene identification and may shed light on the evolvability of organisms.
authors.affiliation: Department of Theoretical Biology, University of Vienna, A-1090 Vienna, Austria philipp.mitteroecker@univie.ac.at.
authors.forename: Philipp
authors.initials: P
authors.lastname: Mitteroecker
authors.affiliation: Department of Biology, Loyola University of Chicago, Chicago, Illinois.
authors.forename: James M
authors.initials: JM
authors.lastname: Cheverud
authors.affiliation: Department of Pediatrics, Cincinnati Children's Hospital Medical Centre, Cincinnati, Ohio.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Pavlicev
mesh_terms: D000465:Algorithms; D000818:Animals; D002874:Chromosome Mapping; D004843:Epistasis, Genetic; D056726:Genetic Association Studies; D014644:Genetic Variation; D016678:Genome; D023281:Genomics / Q000379:methods; D051379:Mice; D008957:Models, Genetic; D015999:Multivariate Analysis
keywords: genetic mapping; genotype–phenotype map; mouse; multivariate analysis; partial least squares