title: Selective and Sensitive Detection of Heavy Metal Ions in 100% Aqueous Solution and Cells with a Fluorescence Chemosensor Based on Peptide Using Aggregation-Induced Emission.
abstract: A fluorescent peptidyl chemosensor for the detection of heavy metal ions in aqueous solution as well as in cells was synthesized on the basis of the peptide receptor for the metal ions using an aggregation-induced emission fluorophore. The peptidyl chemosensor (1) bearing tetraphenylethylene fluorophore showed an exclusively selective turn-on response to Hg(2+) among 16 metal ions in aqueous buffered solution containing NaCl. The peptidyl chemosensor complexed Hg(2+) ions and then aggregated in aqueous buffered solution, resulting in the significant enhancement (OFF-On) of emissions at around 470 nm. The fluorescent sensor showed a highly sensitive response to Hg(2+), and about 1.0 equiv of Hg(2+) was enough for the saturation of the emission intensity change. The detection limit (5.3 nM, R(2) = 0.99) of 1 for Hg(2+) ions was lower than the maximum allowable level of Hg(2+) in drinking water by EPA. Moreover, the peptidyl chemosensor penetrated live cells and detected intracellular Hg(2+) ions by the turn-on response.
authors.affiliation: Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University , Incheon 402-751, South Korea.
authors.forename: Lok Nath
authors.initials: LN
authors.lastname: Neupane
authors.affiliation: Department of Biomedical Sciences, Inha University College of Medicine , Incheon 402-751, South Korea.|Hypoxia-related Disease Research Center, College of Medicine, Inha University , Incheon 402-751, South Korea.
authors.forename: Eun-Taex
authors.initials: ET
authors.lastname: Oh
authors.affiliation: Hypoxia-related Disease Research Center, College of Medicine, Inha University , Incheon 402-751, South Korea.|Department of Microbiology, Inha Research Institute for Medical Science, College of Medicine, Inha University , Incheon 402-751, South Korea.
authors.forename: Heon Joo
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Bioorganic Chemistry Laboratory, Center for Design and Applications of Molecular Catalysts, Department of Chemistry and Chemical Engineering, Inha University , Incheon 402-751, South Korea.
authors.forename: Keun-Hyeung
authors.initials: KH
authors.lastname: Lee
mesh_terms: D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D019216:Metals, Heavy / Q000032:analysis*; D010455:Peptides / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water
chemical_list: D019216:Metals, Heavy; D010455:Peptides; D012996:Solutions; D014867:Water