title: Assessment of genetic fidelity and composition: Mixed elicitors enhance triterpenoid and flavonoid biosynthesis of Glycyrrhiza uralensis Fisch. tissue cultures.
abstract: Glycyrrhiza uralensis has acquired significant importance due to its medicinal properties and health function. In this study, the quality of G. uralensis adventitious roots was evaluated in terms of genetic stability, active compounds, and anti-inflammatory activity. Monomorphic banding pattern obtained from the mother plant and tissue cultures of G. uralensis with randomly amplified polymorphic DNA markers confirmed the genetic stability of adventitious roots. Neoliquiritin (neoisoliquiritin), ononin, liquiritin, and glycyrrhizic acid were identified from G. uralensis adventitious roots on the basis of high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry analysis. This study also revealed that adventitious roots possessed a better anti-inflammatory effect than native roots. To increase the contents of G. uralensis active components, elicitors were used in the adventitious roots culture. The combination of methyl jasmonate and phenylalanine synergistically stimulated the accumulation of glycyrrhetinic acid (0.22 mg/g) and total flavonoid (5.43 mg/g) compared with single treatment. In conclusion, G. uralensis adventitious roots can be an exploitable system for the production of licorice.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Tianjin ZhongXin Pharmaceuticals R&D Center, Tianjin, People's Republic of China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Hongfa
authors.initials: H
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People's Republic of China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
mesh_terms: D018929:Cell Culture Techniques; D005419:Flavonoids / Q000096:biosynthesis* / Q000235:genetics; D029882:Glycyrrhiza uralensis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D013729:Terpenes / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D013729:Terpenes
keywords: Glycyrrhiza uralensis Fisch; HPLC-ESI-MS/MS; RAPD; anti-inflammatory; elicitor