title: Structural Analysis of the Streptomyces avermitilis CYP107W1-Oligomycin A Complex and Role of the Tryptophan 178 Residue.
abstract: CYP107W1 from Streptomyces avermitilis is a cytochrome P450 enzyme involved in the biosynthesis of macrolide oligomycin A. A previous study reported that CYP107W1 regioselectively hydroxylated C12 of oligomycin C to produce oligomycin A, and the crystal structure of ligand free CYP107W1 was determined. Here, we analyzed the structural properties of the CYP107W1-oligomycin A complex and characterized the functional role of the Trp178 residue in CYP107W1. The crystal structure of the CYP107W1 complex with oligomycin A was determined at a resolution of 2.6 Å. Oligomycin A is bound in the substrate access channel on the upper side of the prosthetic heme mainly by hydrophobic interactions. In particular, the Trp178 residue in the active site intercalates into the large macrolide ring, thereby guiding the substrate into the correct binding orientation for a productive P450 reaction. A Trp178 to Gly mutation resulted in the distortion of binding titration spectra with oligomycin A, whereas binding spectra with azoles were not affected. The Gly178 mutant's catalytic turnover number for the 12-hydroxylation reaction of oligomycin C was highly reduced. These results indicate that Trp178, located in the open pocket of the active site, may be a critical residue for the productive binding conformation of large macrolide substrates.
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701 Korea.
authors.forename: Songhee
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701 Korea.
authors.forename: Tan-Viet
authors.initials: TV
authors.lastname: Pham
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701 Korea.
authors.forename: Joo-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701 Korea.
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Lim
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701 Korea.
authors.forename: Hyoung-Goo
authors.initials: HG
authors.lastname: Park
authors.affiliation: School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Gun-Su
authors.initials: GS
authors.lastname: Cha
authors.affiliation: School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea.
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Yun
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Chun
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701 Korea.
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
authors.affiliation: Department of Biological Sciences, Konkuk University, Seoul 143-701 Korea.
authors.forename: Donghak
authors.initials: D
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D009840:Oligomycins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*; D014364:Tryptophan / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009840:Oligomycins; C031004:oligomycin A; D014364:Tryptophan; D003577:Cytochrome P-450 Enzyme System
keywords: CYP; CYP107W1; P450; Streptomyces avermitilis; X-ray crystal structure; oligomycin