title: Encapsulated Laccases for the Room-Temperature Oxidation of Aromatics: Towards Synthetic Low-Molecular-Weight Lignins.
abstract: A new approach for the encapsulation of laccases with enhanced activity and stability by biomimetic silica mineralisation is reported. A range of lignin model compounds, which includes syringol, syringyl acid, 4-vinylphenol, gallic acid, vanillic acid and guaiacol, was oxidised to lignin-type polymers by the silica-immobilised laccase systems at room temperature. The oxidation rate of the immobilised systems was lower than that of the free enzyme counterparts, but interesting products were observed with the new bio-catalytic materials, which showed reusability and good stability.
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milano, Italy. lucia.pistone@icrm.cnr.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Pistone
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR, Via Mario Bianco 9, 20131, Milano, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Ottolina
authors.affiliation: Departamento de Quimica Organica, Universidad de Cordoba, Edificio Marie Curie(C-3), Ctra Nnal IV-A, Km 396, E14014, Córdoba, Spain.
authors.forename: Sudipta
authors.initials: S
authors.lastname: De
authors.affiliation: Departamento de Quimica Organica, Universidad de Cordoba, Edificio Marie Curie(C-3), Ctra Nnal IV-A, Km 396, E14014, Córdoba, Spain.
authors.forename: Antonio A
authors.initials: AA
authors.lastname: Romero
authors.affiliation: Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av da República, 2780-157, Oeiras, Portugal.
authors.forename: Lígia O
authors.initials: LO
authors.lastname: Martins
authors.affiliation: Departamento de Quimica Organica, Universidad de Cordoba, Edificio Marie Curie(C-3), Ctra Nnal IV-A, Km 396, E14014, Córdoba, Spain.
authors.forename: Rafael
authors.identifier: http://orcid.org/0000-0003-4190-1916
authors.initials: R
authors.lastname: Luque
mesh_terms: D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D012997:Solvents / Q000737:chemistry
chemical_list: D012997:Solvents; D008031:Lignin; D042845:Laccase
keywords: biomimetic synthesis; enzyme catalysis; enzymes; immobilization; oxidation