title: Integrated molecular, physiological and in silico characterization of two Halomonas isolates from industrial brine.
abstract: Two haloalkaliphilic bacteria isolated from industrial brine solutions were characterized via molecular, physiological, and in silico metabolic pathway analyses. Genomes from the organisms, designated Halomonas BC1 and BC2, were sequenced; 16S ribosomal subunit-based phylogenetic analysis revealed a high level of similarity to each other and to Halomonas meridiana. Both strains were moderate halophiles with near optimal specific growth rates (≥60 % μ max) observed over <0.1-5 % (w/v) NaCl and pH ranging from 7.4 to 10.2. Isolate BC1 was further characterized by measuring uptake or synthesis of compatible solutes under different growth conditions; in complex medium, uptake and accumulation of external glycine betaine was observed while ectoine was synthesized de novo in salts medium. Transcriptome analysis of isolate BC1 grown on glucose or citrate medium measured differences in glycolysis- and gluconeogenesis-based metabolisms, respectively. The annotated BC1 genome was used to build an in silico, genome-scale stoichiometric metabolic model to study catabolic energy strategies and compatible solute synthesis under gradients of oxygen and nutrient availability. The theoretical analysis identified energy metabolism challenges associated with acclimation to high salinity and high pH. The study documents central metabolism data for the industrially and scientifically important haloalkaliphile genus Halomonas.
authors.affiliation: Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, 59717, USA. ross.carlson@montana.edu.|Center for Biofilm Engineering, Montana State University, Bozeman, MT, 59717, USA. ross.carlson@montana.edu.
authors.forename: Ross P
authors.initials: RP
authors.lastname: Carlson
authors.affiliation: Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, 59717, USA.|Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
authors.forename: Olusegun
authors.initials: O
authors.lastname: Oshota
authors.affiliation: Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, 59717, USA.|U.S. Navy, Washington, DC, USA.
authors.forename: Matt
authors.initials: M
authors.lastname: Shipman
authors.affiliation: Procter and Gamble Co., Cincinnati, OH, 45202, USA.
authors.forename: Justin A
authors.initials: JA
authors.lastname: Caserta
authors.affiliation: Procter and Gamble Co., Cincinnati, OH, 45202, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.affiliation: Procter and Gamble Co., Cincinnati, OH, 45202, USA.
authors.forename: Charles W
authors.initials: CW
authors.lastname: Saunders
authors.affiliation: Procter and Gamble Co., Cincinnati, OH, 45202, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, 59717, USA.|Center for Biofilm Engineering, Montana State University, Bozeman, MT, 59717, USA.
authors.forename: Zackary J
authors.initials: ZJ
authors.lastname: Jay
authors.affiliation: Procter and Gamble Co., Cincinnati, OH, 45202, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Reeder
authors.affiliation: Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, 59717, USA.|Center for Biofilm Engineering, Montana State University, Bozeman, MT, 59717, USA.
authors.forename: Abigail
authors.initials: A
authors.lastname: Richards
authors.affiliation: Procter and Gamble Co., Cincinnati, OH, 45202, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Pettigrew
authors.affiliation: Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, 59717, USA. bpeyton@montana.edu.|Center for Biofilm Engineering, Montana State University, Bozeman, MT, 59717, USA. bpeyton@montana.edu.
authors.forename: Brent M
authors.initials: BM
authors.lastname: Peyton
mesh_terms: D004734:Energy Metabolism; D020632:Halomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D055442:Metabolome; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D055049:Salt Tolerance; D059467:Transcriptome
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Brine; Compatible solute; Halomonas; Halophile; Systems biology