title: Association Between MIF-AS rs755622 and Nephrolithiasis Risk in a Chinese Population.
abstract: BACKGROUND Single-nucleotide polymorphisms (SNPs) located at lncRNA may affect the stability and splicing processes of mRNA formation, which result in the alteration of its interacting partners. The SNP rs755622 within exon of antisense lncRNA MIF- AS and promoter of MIF was implicated in renal disease risk. MATERIAL AND METHODS In this case-control study, we genotyped the SNP rs755622 in 230 patients diagnosed with nephrolithiasis and 250 controls in a Chinese population. RESULTS We found that the rs755622 CG and CC genotypes had a significantly increased nephrolithiasis risk (adjusted OR=1.52, 95% CI=1.03-2.25; OR=2.63, 95% CI=1.21-5.72, P=0.015), compared with GG genotype in the additive model. The rs755622 C carriers (GC/CC) had an adjusted OR (95% CI) of 1.65 (1.14-2.39, P=0.016), compared with the GG genotype in the dominant model. This hazardous effect was more pronounced in subgroup age >46, BMI >24, hypertension, ever smoking, and ever drinking subjects. Moreover, we found that rs755622 could modulate the function of MIF-AS by influencing its folding. CONCLUSIONS These results indicate that the MIF-AS rs755622 polymorphism may have a crucial role in the development of nephrolithiasis.
authors.affiliation: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Gaoxiang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Qinbo
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Department of Urology, Huaiyin Hospital of Huai'an City, Huai'an, Jiangsu, China (mainland).
authors.forename: Qiangdong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Mulong
authors.initials: M
authors.lastname: Du
authors.affiliation: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chu
authors.affiliation: Department of Urology, Huaiyin Hospital of Huai'an City, Huai'an, Jiangsu, China (mainland).
authors.forename: Zhenjia
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Urology, Huaiyin Hospital of Huai'an City, Huai'an, Jiangsu, China (mainland).
authors.forename: Xu
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Meilin
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Chao
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Changjun
authors.initials: C
authors.lastname: Yin
authors.affiliation: Department of Environmental Genomics, iangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Zhengdong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003198:Computer Simulation; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D053040:Nephrolithiasis / Q000235:genetics*; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D012307:Risk Factors
chemical_list: D062085:RNA, Long Noncoding; C000611955:long non-coding RNA MIF-AS, human