title: Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source.
abstract: Multicolor multiphoton microscopy is experimentally demonstrated for the first time on a spectral bandwidth of excitation of 300 nm (full width half maximum) thanks to the implementation a nanosecond supercontinuum (SC) source compact and simple with a low repetition rate. The interest of such a wide spectral bandwidth, never demonstrated until now, is highlighted in vivo: images of glioma tumor cells stably expressing eGFP grafted on the brain of a mouse and its blood vessels network labelled with Texas Red(®) are obtained. These two fluorophores have a spectral bandwidth covering the whole 300 nm available. In parallel, a similar image quality is obtained on a sample of mouse muscle in vitro when excited with this nanosecond SC source or with a classical high rate, femtosecond and quasi monochromatic laser. This opens the way for (i) a simple and very complete biological characterization never performed to date with multiphoton processes, (ii) multiple means of contrast in nonlinear imaging allowed by the use of numerous fluorophores and (iii) other multiphoton processes like three-photon ones.
authors.affiliation: Université de Limoges, CNRS UMR 7252, Labex "Sigma-Lim", INRA UMR 1061, F-87000, Limoges, France. claire.lefort@xlim.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Lefort
authors.affiliation: Université de Limoges, CNRS UMR 7252, Labex "Sigma-Lim", INRA UMR 1061, F-87000, Limoges, France.
authors.forename: Rodney P
authors.initials: RP
authors.lastname: O'Connor
authors.affiliation: Université de Limoges, CNRS UMR 7252, Labex "Sigma-Lim", INRA UMR 1061, F-87000, Limoges, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Blanquet
authors.affiliation: Université de Limoges, CNRS UMR 7252, Labex "Sigma-Lim", INRA UMR 1061, F-87000, Limoges, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Magnol
authors.affiliation: Institute of Applied Physics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kano
authors.affiliation: LEUKOS Innovative Optical Systems, 37 rue Henri Giffard, Z.I. Nord, 87280, Limoges, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Tombelaine
authors.affiliation: Université de Limoges, CNRS UMR 7252, Labex "Sigma-Lim", INRA UMR 1061, F-87000, Limoges, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lévêque
authors.affiliation: Université de Limoges, CNRS UMR 7252, Labex "Sigma-Lim", INRA UMR 1061, F-87000, Limoges, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Couderc
authors.affiliation: Université de Limoges, CNRS UMR 7252, Labex "Sigma-Lim", INRA UMR 1061, F-87000, Limoges, France.|LEUKOS Innovative Optical Systems, 37 rue Henri Giffard, Z.I. Nord, 87280, Limoges, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Leproux
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D005910:Glioma / Q000000981:diagnostic imaging*; D007834:Lasers; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D018482:Muscle, Skeletal / Q000000981:diagnostic imaging
keywords: multicolor imaging; multiphoton microscopy; nanosecond supercontinuum source; nonlinear imaging