title: Comparison of Dextran Perfusion and GSI-B4 Isolectin Staining in a Mouse Model of Oxygen-induced Retinopathy.
abstract: OBJECTIVE
  Oxygen-induced retinopathy (OIR) is a robust and widely used animal model for the study of retinal neovascularization (NV). Dextran perfusion and Griffonia simplicifolia isolectin B4 (GSI-B4) staining are two common methods for examining the occurrence and extent of OIR. This study provides a quantitative comparison of the two for OIR detection.
  
  
  METHODS
  At postnatal day 7 (PN7), fifteen C57BL/6J mice were exposed to a 75% hyperoxic condition for 5 days and then returned to room air conditions. At PN17, the mice received intravitreal injection of GSI-B4 Alexa Fluor 568 conjugate. After 10 hours, they were infused with FITC-dextran conjugate via the left ventricle. Retinal flat mounts were photographed by confocal microscopy. Areas with fluorescent signals and the total retinal areas were quantified by Image J software.
  
  
  RESULTS
  Both GSI-B4 and dextran detected the peripheral neovascular area. The mean hyper fluorescence area was 0.33 ± 0.14% of whole retinal area determined by GSI-B4 staining and 0.25 ± 0.28% determined by dextran perfusion. The difference between the two measures was 0.08% (95% CI:-0.59%, 0.43%). The Pearson correlation coefficient between the two methods was 0.386,P =0.035. The mean coincidence rates were 14.3 ± 13.4% and 24.9 ± 18.5% for GSI-B4 and dextran staining, respectively.
  
  
  CONCLUSIONS
  Both methods can complement each other in demonstrating and quantitatively evaluating retinal NV. A poor agreement was found between the two methods; GSI-B4 isolectin was more effective than FITC-dextran perfusion in evaluating the extent of retinal NV in a mouse model of OIR.
authors.forename: Shaofen
authors.initials: S
authors.lastname: Huang
authors.forename: Jiajian
authors.initials: J
authors.lastname: Liang
authors.forename: Gary Hin-Fai
authors.initials: GH
authors.lastname: Yam
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Lu
authors.forename: Chi Pui
authors.initials: CP
authors.lastname: Pang
authors.forename: Haoyu
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D004396:Coloring Agents; D003911:Dextrans; D004195:Disease Models, Animal; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D058449:Intravitreal Injections; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000633:toxicity; D037121:Plant Lectins; D015861:Retinal Neovascularization / Q000139:chemically induced / Q000175:diagnosis*
chemical_list: D004396:Coloring Agents; D003911:Dextrans; C045475:Griffonia simplicifolia lectins; D037121:Plant Lectins; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate; D010100:Oxygen