title: Lentiviral vector PLV-PI3KCG gene transfer inhibits hypoxic cardiomyocytes apoptosis.
abstract: The PI3K/Akt signal pathway was suggested to be associated with apoptosis. However, it was still unclear whether activated PI3K/Akt signaling pathway could inhibit hypoxic cardiomyocytes apoptosis. In this research, the recombinant PI3KCG lentiviral vector plasmid (PLV-PI3KCG) was constructed and transfected into neonatal rat hypoxia/reoxygenation (H/R) injury cardiomyocytes models which were randomly divided into five groups as the normal control group, H/R group, HR empty plasmid group (HRE group), HR PLV-PI3KCG transfection preconditioning group (HRP group), and HR PLV-PI3KCG transfection + LY294002 group (HRPL group). Compared with the H/R, HRE and HRPL groups, the cardiomyocytes beat frequency and survival rate in the HRP group were significantly increased (P<0.05) and the released LDH were significantly decreased (P<0.05). The Bcl-2/Bax ratio was significantly lower in H/R, HRE and HRPL groups than that in HRP group (P<0.05). Activated PI3K/Akt signaling pathway could play a protection role in the cardiomyocytes H/R injury process which could be inhibited by LY294002.
authors.affiliation: Department of Geriatrics, First Affiliated Hospital of Nanjing Medical University Nanjing 210029, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Li
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029, China; Department of Cardiology, Laiwu Steel Group HospitalLaiwu 271126, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Nanjing Medical University Nanjing 210029, China.
authors.forename: Xin-Zheng
authors.initials: XZ
authors.lastname: Lu
keywords: Lentiviral vector; PI3KCG; cardiomyocytes; hypoxia/reoxygenation; virus packaging