title: Pan-proteomics, a concept for unifying quantitative proteome measurements when comparing closely-related bacterial strains.
abstract: The comparison of proteomes between genetically heterogeneous bacterial strains may offer valuable insights into physiological diversity and function, particularly where such variation aids in the survival and virulence of clinically-relevant strains. However, reports of such comparisons frequently fail to account for underlying genetic variance. As a consequence, the current knowledge regarding bacterial physiological diversity at the protein level may be incomplete or inaccurate. To address this, greater consideration must be given to the impact of genetic heterogeneity on proteome comparisons. This may be possible through the use of pan-proteomics, an analytical concept that permits the ability to qualitatively and quantitatively compare the proteomes of genetically heterogeneous organisms. Limited examples of this emerging technology highlight currently unmet analytical challenges. In this article we define pan-proteomics, where its value lies in microbiology, and discuss the technical considerations critical to its successful execution and potential future application.
authors.affiliation: a Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health , Queensland University of Technology , Brisbane , Australia.|b Molecular Microbiological Pathogenesis Group, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health , Queensland University of Technology , Brisbane , Australia.
authors.forename: James A
authors.initials: JA
authors.lastname: Broadbent
authors.affiliation: a Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health , Queensland University of Technology , Brisbane , Australia.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Broszczak
authors.affiliation: b Molecular Microbiological Pathogenesis Group, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health , Queensland University of Technology , Brisbane , Australia.
authors.forename: Imalka U K
authors.initials: IU
authors.lastname: Tennakoon
authors.affiliation: b Molecular Microbiological Pathogenesis Group, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Faculty of Health , Queensland University of Technology , Brisbane , Australia.
authors.forename: Flavia
authors.initials: F
authors.lastname: Huygens
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018740:Genetic Heterogeneity; D016680:Genome, Bacterial; D010802:Phylogeny; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome
keywords: Bacterial strains; Bioinformatics; Clinical microbiology; Comparative proteomics; Genetic heterogeneity; Microbiology; Pan-proteomics