title: Comparative effects of catechin, epicatechin and N-Ω-nitroarginine on quinolinic acid-induced oxidative stress in rat striatum slices.
abstract: The aim of this work was to compare the effects of catechin (CAT), epicatechin (EPI) and N-ω-l-nitroarginine (L-NARG) on different endpoints of oxidative stress induced by quinolinic acid (QUIN) in a simple tissue preparation, rat striatal slices - with particular emphasis in the glutathione system - in order to provide revealing information on the antioxidant efficacy of these agents in an excitotoxic model.
  
  
  METHODS
  Rat striatal slices were incubated for 1h in the presence of 100 μM QUIN and/or 85 μM CAT or EPI, or 100 μM L-NARG. Lipid peroxidation (LP) and the levels of reduced and oxidized glutathione (GSH and GSSG) were determined.
  
  
  RESULTS
  The three agents tested completely blocked the QUIN-induced lipid peroxidation and recovered the QUIN-induced altered GSH/GSSG balance. No statistical differences were detected among the protective effects exerted by these antioxidants, suggesting similar efficacy and common antioxidant mechanisms. The antioxidant properties exhibited by these molecules on the excitotoxic model tested herein support an active role of glutathione and prompt their use as therapeutic tools in models of neurodegenerative disorders.
authors.affiliation: Laboratorio de Neurociencias, Instituto Nacional de Pediatría, Mexico City, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Santamaría-Del Ángel
authors.affiliation: Laboratorio de Neurociencias, Instituto Nacional de Pediatría, Mexico City, Mexico.
authors.forename: Norma Angélica
authors.initials: NA
authors.lastname: Labra-Ruíz
authors.affiliation: Laboratorio de Neurociencias, Instituto Nacional de Pediatría, Mexico City, Mexico.
authors.forename: Mercedes Edna
authors.initials: ME
authors.lastname: García-Cruz
authors.affiliation: Laboratorio de Neurociencias, Instituto Nacional de Pediatría, Mexico City, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Calderón-Guzmán
authors.affiliation: Laboratorio de Neurociencias, Instituto Nacional de Pediatría, Mexico City, Mexico.
authors.forename: Armando
authors.initials: A
authors.lastname: Valenzuela-Peraza
authors.affiliation: Laboratorio de Farmacología, Instituto Nacional de Pediatría, Mexico City, Mexico; Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico. Electronic address: juarezol@yahoo.com.
authors.forename: Hugo
authors.initials: H
authors.lastname: Juárez-Olguín
mesh_terms: D000818:Animals; D002392:Catechin / Q000494:pharmacology*; D003342:Corpus Striatum / Q000473:pathology*; D019803:Glutathione Disulfide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000737:chemistry; D008297:Male; D019335:Nitroarginine / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017378:Quinolinic Acid / Q000633:toxicity*; D017208:Rats, Wistar
chemical_list: D008055:Lipids; D019335:Nitroarginine; D002392:Catechin; D017378:Quinolinic Acid; D019803:Glutathione Disulfide
keywords: Brain; Excitotoxicity; Flavonoids; Gluthation; Lipid Peroxidation; Nitric oxide; Oxidative damage