title: Cloning of TaSST genes associated with water soluble carbohydrate content in bread wheat stems and development of a functional marker.
abstract: We cloned TaSST genes, developed a gene-specific marker for TaSST-D1, and identified three QTL in the Doumai/Shi 4185 RIL population. TaSST-D1 is within one of the three QTL. Sucrose:sucrose-1-fructosyltransferase (1-SST), a critical enzyme in the fructan biosynthetic pathway, is significantly and positively associated with water soluble carbohydrate (WSC) content in bread wheat stems. In the present study, wheat 1-SST genes (TaSST) were isolated and located on chromosomes 4A, 7A and 7D. Sequence analysis of TaSST-D1 revealed 15 single nucleotide polymorphisms (SNP) in the third exon between cultivars with higher and lower WSC content. A cleaved amplified polymorphism sequence (CAPS) marker, WSC7D, based on the polymorphism at position 1216 (C-G) was developed to discriminate the two alleles. WSC7D was located on chromosome 7DS using a recombinant inbred line (RIL) population from a Doumai/Shi 4185 cross, and a set of Chinese Spring nullisomic-tetrasomic lines. TaSST-D1 co-segregated with the CAPS marker WSC7D and was linked to SNP marker BS00108793_51 on chromosome 7DS at a genetic distance of 6.1 cM. It explained 8.8, 10.9, and 11.3% of the phenotypic variances in trials at Beijing and Shijiazhuang as well as the averaged data from those environments, respectively. Two additional QTL (QWSC.caas-4BS and QWSC.caas-7AS) besides TaSST-D1 were mapped in the RIL population. One hundred and forty-nine Chinese wheat cultivars and advanced lines tested in four environments were used to validate a highly significant (P < 0.01) association between WSC7D and WSC content in wheat stems. WSC7D can be used as a gene-specific marker for improvement of stem WSC content in wheat breeding programs.
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
authors.forename: Yonggui
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Cotton Research Institute, CAAS, Huanghedadao, Anyang, Henan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
authors.forename: Jindong
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, 830052, Urumqi, Xinjiang, China.
authors.forename: Weie
authors.initials: W
authors.lastname: Wen
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
authors.forename: Ruilian
authors.initials: R
authors.lastname: Jing
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China. xiaxianchun@caas.cn.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Xia
authors.affiliation: National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.|International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, 100081, Beijing, China.
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: He
mesh_terms: D000483:Alleles; D002241:Carbohydrates / Q000737:chemistry*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D006602:Hexosyltransferases / Q000235:genetics*; D010641:Phenotype; D000069600:Plant Breeding; D018547:Plant Stems / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D014867:Water; D001426:Bacterial Proteins
chemical_list: D002241:Carbohydrates; D018744:DNA, Plant; D005819:Genetic Markers; D006602:Hexosyltransferases; D014867:Water; C021451:inulosucrase; D001426:Bacterial Proteins