title: Autotetraploid cell line induced by SP600125 from crucian carp and its developmental potentiality.
abstract: Polyploidy has many advantages over diploidy, such as rapid growth, sterility, and disease resistance, and has been extensively applied in agriculture and aquaculture. Though generation of new polyploids via polyploidization has been achieved in plants by different ways, it is comparatively rare in animals. In this article, by a chemical compound, SP600125, polyploidization is induced in fish cells in vitro, and a stable autotetraploid cell line has been generated from diploid fibroblast cells of crucian carp. As a c-Jun N-terminal kinase (Jnk) inhibitor, SP600125 does not function during the induction process of polyploidization. Instead, the p53 signal pathway might be involved. Using the SP600125-induced tetraploid cells and eggs of crucian carp as the donors and recipients, respectively, nuclear transplantation was conducted such that tetraploid embryos were obtained. It suggests that combining polyploidization and the somatic cell nuclear transfer technique (SCNT) is an efficient way to generate polyploidy, and the presented method in this research for generating the tetraploid fish from diploid fish can provide a useful platform for polyploid breeding.
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.|State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.|State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Minggui
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Liangyue
authors.initials: L
authors.lastname: Peng
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wan
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Rurong
authors.initials: R
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: The National Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Lab of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000873:Anthracenes / Q000494:pharmacology*; D017756:Aquaculture / Q000379:methods; D002347:Carps / Q000235:genetics*; D002460:Cell Line; D004171:Diploidy; D004625:Embryo, Nonmammalian; D005260:Female; D029941:Fish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018703:Founder Effect; D005786:Gene Expression Regulation; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D007621:Karyotyping; D008297:Male; D009153:Mutagens / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D057891:Tetraploidy; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D015053:Zygote / Q000254:growth & development / Q000378:metabolism
chemical_list: D000873:Anthracenes; D029941:Fish Proteins; D009153:Mutagens; D016159:Tumor Suppressor Protein p53; C432165:pyrazolanthrone; D048031:JNK Mitogen-Activated Protein Kinases