title: p11 mediates the BDNF-protective effects in dendritic outgrowth and spine formation in B27-deprived primary hippocampal cells.
abstract: BACKGROUND
  p11 (S100A10) is a key regulator of depression-like behaviors and antidepressant drug response in rodent models. Recent studies suggest that p11 mediates the behavioral antidepressant action of brain-derived neurotrophic factor (BDNF) in rodents. BDNF improves neural plasticity, which is linked to the cellular actions of antidepressant drugs. In the present study, we investigated whether p11 regulated BDNF action on neural plasticity in vitro.
  
  
  METHODS
  We generated primary hippocampal cultures. p11 expression, total dendritic length, and spine density were investigated under toxic conditions induced by B27 deprivation, which causes hippocampal cell death.
  
  
  RESULTS
  B27 deprivation significantly decreased p11 expression. Treatment with BDNF significantly prevented the B27 deprivation-induced decrease in p11 levels in a concentration-dependent manner, whereas these concentrations had no effect on control cultures. B27 deprivation significantly reduced the total length of hippocampal dendrites and spine density. BDNF increased the total dendritic length and spine density in conditions with or without B27. Furthermore, p11 knockdown through small interfering RNA (siRNA) transfection blocked these effects. The overexpression of p11 in B27-deprived cells increased the total dendritic length and spine density, and treatment with BDNF potentiated these effects.
  
  
  CONCLUSIONS
  This study should be confirmed in animal models of depression.
  
  
  CONCLUSIONS
  Taken together, our data suggest that BDNF-induced improvement in neural plasticity may depend on the regulation of p11 in hippocampal cells with B27 deprivation. These results provide evidence to strengthen the theoretical basis of a role for p11 in BDNF-induced antidepressant action.
authors.affiliation: Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea; Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea.
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea.
authors.forename: Le Hoa
authors.initials: le H
authors.lastname: Nhu
authors.affiliation: Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea.
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Cho
authors.affiliation: Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea.
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Seo
authors.affiliation: Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea.
authors.forename: Chan Hong
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea.
authors.forename: Nguyen Ngoc
authors.initials: NN
authors.lastname: Ly
authors.affiliation: Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea.
authors.forename: Cheol Min
authors.initials: CM
authors.lastname: Choi
authors.affiliation: Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea.
authors.forename: Bong Ju
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea.
authors.forename: Gyung-Mee
authors.initials: GM
authors.lastname: Kim
authors.affiliation: InAm Neuroscience Research Center, Wonkwang University, Sanbon Hospital, Gunpo, kyeonggi-do, Republic of Korea.
authors.forename: Wongi
authors.initials: W
authors.lastname: Seol
authors.affiliation: Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea; Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea; Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea. Electronic address: iybihwc@naver.com.
authors.forename: Jung Goo
authors.initials: JG
authors.lastname: Lee
authors.affiliation: Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea; Department of health science and technology, Graduate School of Inje University, Busan, Republic of Korea; Department of Psychiatry, School of Medicine, Haeundae Paik Hospital, Inje University, Busan, Republic of Korea. Electronic address: neuro109@hanmail.net.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000378:metabolism*; D000928:Antidepressive Agents / Q000494:pharmacology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D003712:Dendrites / Q000235:genetics*; D003863:Depression / Q000235:genetics; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009473:Neuronal Plasticity / Q000235:genetics; D009418:S100 Proteins / Q000378:metabolism*; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10
keywords: BDNF; Hippocampus; Spine density; Total dendritic length; p11