title: MicroRNA-9 regulates cardiac fibrosis by targeting PDGFR-β in rats.
abstract: The proliferation of cardiac fibroblasts (CFs) and excessive deposition of extracellular matrix (ECM) are the main pathological characteristics of cardiac fibrosis. In recent years, microRNAs (miRNAs) have been found to be a new kind of regulator in cardiac fibrosis. The purpose of this study was to investigate the role of microRNA-9 (miR-9) in the process of cardiac fibrosis and its mechanism. Treatment of cultured neonatal rat CFs with PDGF-BB or serum suppressed the expression of miR-9. Overexpression of miR-9 obviously inhibited neonatal rat CFs proliferation and collagen production as detected by MTT assays, qRT-PCR, and western blotting. The effects of miR-9 in CFs were abrogated by co-transfection with miR-9 inhibitors. Overexpression of miR-9 reduced the mRNA and protein levels of PDGFR-βand its downstream protein, extracellular signal-regulated kinase (ERK) 1/2. Silencing PDGFR-βby small interfering RNA mimicked the anti-fibrotic action of miR-9, whereas overexpression of PGDFR-β canceled the effect of miR-9 in cultured CFs. Dual-luciferase reporter assays showed that PDGFR-βwas a direct target of miR-9. Overexpression of miR-9 inhibited cardiac fibrosis by targeting PDGFR-β, indicating that miR-9 might play a role in the treatment of cardiac fibrosis.
authors.affiliation: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University, No. 17 Lujiang Road, Hefei, 230001, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University, No. 17 Lujiang Road, Hefei, 230001, China. lkma119@163.com.
authors.forename: LiKun
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University, No. 17 Lujiang Road, Hefei, 230001, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University, No. 17 Lujiang Road, Hefei, 230001, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University, No. 17 Lujiang Road, Hefei, 230001, China.
authors.forename: LongWei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Cardiology, Anhui Provincial Hospital Affiliated to Anhui Medical University, No. 17 Lujiang Road, Hefei, 230001, China.
authors.forename: HanZhang
authors.initials: H
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions / Q000187:drug effects; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D000070416:Antagomirs / Q000378:metabolism / Q000494:pharmacology; D000077214:Becaplermin; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D004719:Endomyocardial Fibrosis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter / Q000187:drug effects; D020935:MAP Kinase Signaling System / Q000187:drug effects; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D058628:Myofibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D034622:RNA Interference / Q000187:drug effects; D034741:RNA, Small Interfering; D017207:Rats, Sprague-Dawley; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D020413:3' Untranslated Regions; D043925:Angiogenesis Inducing Agents; D000070416:Antagomirs; C582333:MIRN9 microRNA, rat; D035683:MicroRNAs; D020574:Proto-Oncogene Proteins c-sis; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D000077214:Becaplermin; D003094:Collagen; D020797:Receptor, Platelet-Derived Growth Factor beta
keywords: Cardiac fibroblasts; Cardiac fibrosis; PDGFR-β; miRNA-9