title: Comparative Transcriptomic Analysis of Primary Duck Hepatocytes Provides Insight into Differential Susceptibility to DHBV Infection.
abstract: Primary duck hepatocytes (PDH) displays differential susceptibility to duck hepatitis B virus when maintained in the media supplemented with fetal bovine serum or dimethyl sulfoxide (DMSO) which has been widely used for the maintenance of hepatocytes, and prolonging susceptibility to hepadnavirus. However the mechanism underlying maintenance of susceptibility to hepadnavirus by DMSO treatment remains unclear. In this study, a global transcriptome analysis of PDHs under different culture conditions was conducted for investigating the effects of DMSO on maintenance of susceptibility of PDH to DHBV in vitro. The 384 differential expressed genes (DEGs) were identified by comparisons between each library pair (PDHs cultured with or without DMSO or fresh isolated PDH). We analyzed canonical pathways in which the DEGs were enriched in Hepatic Fibrosis / Hepatic Stellate Cell Activation, Bile Acid Biosynthesis and Tight Junction signaling. After re-annotation against human genome data, the 384 DEGs were pooled together with proteins belonging to hepatitis B pathway to construct a protein-protein interaction network. The combination of decreased expression of liver-specific genes (CYP3A4, CYP1E1, CFI, RELN and GSTA1 et al) with increased expression of hepatocyte-dedifferentiation-associated genes (PLA2G4A and PLCG1) suggested that in vitro culture conditions results in the fading of hepatocyte phenotype in PDHs. The expression of seven DEGs associated with tight junction formation (JAM3, PPP2R2B, PRKAR1B, PPP2R2C, MAGI2, ACTA2 and ACTG2) was up-regulated after short-term culture in vitro, which was attenuated in the presence of DMSO. Those results could shed light on DHBV infection associated molecular events affected by DMSO.
authors.affiliation: Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yan
authors.affiliation: Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.|Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
authors.forename: Su
authors.initials: S
authors.lastname: Qu
authors.affiliation: Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Ding
authors.affiliation: Department of Laboratory Medicine, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yixue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.
authors.forename: Junqi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.
authors.forename: Di
authors.initials: D
authors.lastname: Qu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016000:Cluster Analysis; D004279:DNA, Viral / Q000378:metabolism; D004198:Disease Susceptibility; D004372:Ducks / Q000235:genetics / Q000821:virology*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D018347:Hepadnaviridae Infections / Q000235:genetics* / Q000662:veterinary* / Q000821:virology; D015611:Hepatitis B Virus, Duck / Q000502:physiology*; D006524:Hepatitis, Viral, Animal / Q000235:genetics*; D022781:Hepatocytes / Q000378:metabolism / Q000821:virology*; D025341:Principal Component Analysis; D025941:Protein Interaction Mapping; D060888:Real-Time Polymerase Chain Reaction; D000091042:Reelin Protein; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral; D000091042:Reelin Protein; C000717127:RELN protein, human