title: A zwitterionic 1D/2D polymer co-crystal and its polymorphic sub-components: a highly selective sensing platform for HIV ds-DNA sequences.
abstract: Polymorphic compounds {[Cu(dcbb)2(H2O)2]·10H2O}n (2, 1D chain), [Cu(dcbb)2]n (3, 2D layer) and their co-crystal {[Cu(dcbb)2(H2O)][Cu(dcbb)2]2}n (4) have been prepared from the coordination reaction of a 2D polymer [Na(dcbb)(H2O)]n (1, H2dcbbBr = 1-(3,5-dicarboxybenzyl)-4,4'-bipyridinium bromide) with Cu(NO3)2·3H2O at different temperatures in water. Compounds 2-4 have an identical metal-to-ligand stoichiometric ratio of 1 : 2, but absolutely differ in structure. Compound 3 features a 2D layer structure with aromatic rings, positively charged pyridinium and free carboxylates on its surface, promoting electrostatic, π-stacking and/or hydrogen-bonding interactions with the carboxyfluorescein (FAM) labeled probe single-stranded DNA (probe ss-DNA, delineates as P-DNA). The resultant P-DNA@3 system facilitated fluorescence quenching of FAM via a photoinduced electron transfer process. The P-DNA@3 system functions as an efficient fluorescent sensor selective for HIV double-stranded DNA (HIV ds-DNA) due to the formation of a rigid triplex structure with the recovery of FAM fluorescence. The system reported herein also distinguishes complementary HIV ds-DNA from mismatched target DNA sequences with the detection limit of 1.42 nM.
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, P. R. China. jxchen@smu.edu.cn whchen@smu.edu.cn.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Zhao
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, P. R. China. jxchen@smu.edu.cn whchen@smu.edu.cn.
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Yang
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. whzhang@suda.edu.cn and Institute of Materials Research and Engineering (IMRE), A*STAR, 3 Research Link, 117602, Singapore.
authors.forename: Ni-Ni
authors.initials: NN
authors.lastname: Ding
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, P. R. China. jxchen@smu.edu.cn whchen@smu.edu.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Qin
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, P. R. China. jxchen@smu.edu.cn whchen@smu.edu.cn.
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Qiu
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, P. R. China. jxchen@smu.edu.cn whchen@smu.edu.cn.
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Chen
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China. whzhang@suda.edu.cn and Institute of Materials Research and Engineering (IMRE), A*STAR, 3 Research Link, 117602, Singapore.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, P. R. China. jxchen@smu.edu.cn whchen@smu.edu.cn.
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Chen
authors.affiliation: Institute of Materials Research and Engineering (IMRE), A*STAR, 3 Research Link, 117602, Singapore and Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore. andyhor@hku.hk.
authors.forename: T S Andy
authors.initials: TS
authors.lastname: Hor
mesh_terms: D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005452:Fluoresceins / Q000737:chemistry; D006678:HIV / Q000235:genetics*; D006860:Hydrogen Bonding; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D056831:Coordination Complexes; D005452:Fluoresceins; D011108:Polymers; C024098:6-carboxyfluorescein; D003300:Copper; D004247:DNA