title: Selenoprotein X Gene Knockdown Aggravated H2O2-Induced Apoptosis in Liver LO2 Cells.
abstract: To determine the roles of selenoprotein X gene (Selx) in protecting liver cells against oxidative damage, the influences of Selx knockdown on H2O2-induced apoptosis in human normal hepatocyte (LO2) cells were studied. pSilencer 3.1 was used to develop knockdown vector targeting the 3'-UTR of human Selx. The Selx knockdown and control cells were further exposed to H2O2, and cell viability, cell apoptosis rate, and the expression levels of mRNA and protein of apoptosis-related genes were detected. The results showed that vector targeting the 3'-UTR of Selx successfully silenced mRNA or protein expression of SelX in LO2 cells. Selx knockdown resulted in decreased cell viability, increased percentage of early apoptotic cells, decreased Bcl2A1 and Bcl-2 expression, and increased phosphorylation of P38 in LO2 cells. When Selx knockdown LO2 cells were exposed to H2O2, characteristics of H2O2-induced cell dysfunctions were further exacerbated. Taken together, our findings suggested that SelX played important roles in protecting LO2 cells against oxidative damage and reducing H2O2-induced apoptosis in liver cells.
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Jiayong
authors.initials: J
authors.lastname: Tang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Sichuan Provincial General Station for Animal Husbandry, Chengdu, 610041, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Longqiong
authors.initials: L
authors.lastname: Wang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Cai
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Shang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Chengdu, Sichuan, 611130, China. zhua666@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002460:Cell Line; D055785:Gene Knockdown Techniques; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D015778:Minor Histocompatibility Antigens / Q000096:biosynthesis / Q000235:genetics; D010084:Oxidation-Reduction / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D051140:Selenoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C096816:BCL2-related protein A1; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; D051140:Selenoproteins; D006861:Hydrogen Peroxide
keywords: Apoptosis; H2O2; Knockdown; LO2; Selenoprotein