title: Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research.
abstract: Skin serves as a protective barrier and also harbors numerous microorganisms collectively comprising the skin microbiome. As a result of recent advances in sequencing (next-generation sequencing), our understanding of microbial communities on skin has advanced substantially. In particular, the 16S ribosomal RNA gene sequencing technique has played an important role in efforts to identify the global communities of bacteria in healthy individuals and patients with various disorders in multiple topographical regions over the skin surface. Here, we describe basic principles, study design, and a workflow of 16S ribosomal RNA gene sequencing methodology, primarily for investigators who are not familiar with this approach. This article will also discuss some applications and challenges of 16S ribosomal RNA sequencing as well as directions for future development.
authors.affiliation: Dermatology Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, Maryland, USA.
authors.forename: Jay-Hyun
authors.initials: JH
authors.lastname: Jo
authors.affiliation: Dermatology Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, Maryland, USA.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Kennedy
authors.affiliation: Dermatology Branch, Center for Cancer Research, National Cancer Institute, National Institute of Health, Bethesda, Maryland, USA. Electronic address: konghe@mail.nih.gov.
authors.forename: Heidi H
authors.initials: HH
authors.lastname: Kong
mesh_terms: D001419:Bacteria / Q000235:genetics*; D005260:Female; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D064307:Microbiota / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D012107:Research Design; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D012867:Skin / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S