title: Transcription factor Fli-1 positively regulates lipopolysaccharide-induced interleukin-27 production in macrophages.
abstract: IL-27 is an important regulator of TLR4-activated innate immune. The mechanism by which IL-27 production is regulated in TLR4-activated innate immune remains largely unclear. Here we show that expression of transcription factor Fli-1 at protein level is increased in macrophages following LPS stimulation. Fli-1 overexpression increases LPS-activated IL-27 production in macrophages. Consistently, Fli-1 knockdown inhibits LPS-induced IL-27 production in macrophages. Chromatin immunoprecipitation (ChIP) assay reveals that Fli-1 binds the promoter of IL-27 p28 subunit. Further experiments manifest that Fli-1 binds the region between -250 and -150 bp upstream of the transcriptional start site of p28 gene and increases p28 gene promoter-controlled transcription. These results demonstrate that Fli-1 positively regulates IL-27 production in TLR4-activated immune response by promoting transcription of IL-27 p28 gene.
authors.affiliation: Cancer Institute, Institute of Translational Medicine, Second Military Medical University, Shanghai 200433, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Cancer Institute, Institute of Translational Medicine, Second Military Medical University, Shanghai 200433, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Ma
authors.affiliation: Cancer Institute, Institute of Translational Medicine, Second Military Medical University, Shanghai 200433, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Cancer Institute, Institute of Translational Medicine, Second Military Medical University, Shanghai 200433, China.
authors.forename: Lingxi
authors.initials: L
authors.lastname: Zhu
authors.affiliation: National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Wen
authors.affiliation: Cancer Institute, Institute of Translational Medicine, Second Military Medical University, Shanghai 200433, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China. Electronic address: liuqy@immunol.org.
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Cancer Institute, Institute of Translational Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: anhz@immunol.org.
authors.forename: Huazhang
authors.initials: H
authors.lastname: An
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D015151:Immunoblotting; D064094:Interleukin-27 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D051789:Proto-Oncogene Protein c-fli-1 / Q000235:genetics / Q000276:immunology*; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D064094:Interleukin-27; D008070:Lipopolysaccharides; D051789:Proto-Oncogene Protein c-fli-1; D034741:RNA, Small Interfering
keywords: Fli-1; Gene transcription; IL-27; TLR