title: Piscidin: Antimicrobial peptide of rock bream, Oplegnathus fasciatus.
abstract: The piscidin family consists of antimicrobial peptides (AMPs) that are mainly found in fish and are crucial effectors of fish innate immune responses. The piscidin family typically has broad-spectrum antimicrobial activity and can modulate immune responses. In this study, we cloned rock bream piscidin (Rbpisc) and investigated its gene expression and biological activity (including antimicrobial and cytotoxic activities). The coding region of Rbpisc consisted of 213 base pairs (bp) encoding 70 amino acid residues. The tertiary structure predicted for Rbpisc includes an amphipathic helix-loop-helix structure. The Rbpisc gene was highly expressed in the gills of healthy fish. The gene expression of Rbpisc increased in the gills after pathogen infection, while the expression was down-regulated in other tissues. A synthetic peptide based on the AMP 12 domain amino acid sequence of Rbpisc appeared to have broad-spectrum antimicrobial activity against various bacteria. However, the synthetic peptide exhibited weak haemolytic activity against fish erythrocytes. These results suggest that Rbpisc might play an important role in the innate immune responses of rock bream.
authors.affiliation: Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Jin-Sol
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Jung
authors.affiliation: Biotechnology Research Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors.forename: Cheul Min
authors.initials: CM
authors.lastname: An
authors.affiliation: Biotechnology Research Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Aquatic Life Disease Control Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors.forename: Seong Don
authors.initials: SD
authors.lastname: Hwang
authors.affiliation: Aquatic Life Disease Control Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors.forename: Mun-Gyeong
authors.initials: MG
authors.lastname: Kwon
authors.affiliation: Aquatic Life Disease Control Division, National Institute of Fisheries Science, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, Republic of Korea.
authors.forename: Myoung-Ae
authors.initials: MA
authors.lastname: Park
authors.affiliation: Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Mu-Chan
authors.initials: MC
authors.lastname: Kim
authors.affiliation: Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005880:Gills / Q000378:metabolism; D007113:Immunity, Innate; D010473:Perciformes / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D000072756:Protein Conformation, alpha-Helical; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; C449802:moronecidin protein, Morone saxatilis
keywords: Antimicrobial activity; Antimicrobial peptides; Cytotoxicity; Piscidin; Rock bream