title: COMP-Ang1 promotes long-term survival of allogeneic islet grafts in a bioinert perforated chamber by inhibiting inflammation via inhibition of the TLR4 signaling pathway.
abstract: OBJECTIVE
  To evaluate the effects of cartilage oligomeric matrix protein (COMP)- angiopoietin-1 (Ang1) on allogeneic islet graft survival in a bioinert perforated chamber.
  
  
  RESULTS
  COMP-Ang1 treatment significantly decreased lipopolysaccharide-induced cell apoptosis and islet-related lymph node cell proliferation (both P < 0.01). Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 levels in the chamber exudate were significantly lower in the COMP-Ang1 + chamber group than in the chamber group (all P < 0.05), as were the protein expression levels. COMP-Ang1 significantly inhibited the expression of Toll-like receptor 4 (TLR4) in cultured islets. Finally, full COMP-Ang1 treatment resulted in the longest survival time among the treatment groups.
  
  
  CONCLUSIONS
  Combined use of the bioinert perforated chamber with COMP-Ang1 is an effective strategy for improving islet allograft survival.
authors.affiliation: Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China.
authors.forename: Dalei
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Pathology and Hepatology, Beijing 302 Hospital, Beijing, 100039, People's Republic of China.
authors.forename: Lina
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China.
authors.forename: Yingzi
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Hao
authors.affiliation: Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Department of Immunology, The Fourth Military Medical University, Xi'an, 710032, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhuang
authors.affiliation: Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Su
authors.affiliation: Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi'an, 710032, People's Republic of China. guoshuzhong1952@126.com.
authors.forename: Shuzhong
authors.initials: S
authors.lastname: Guo
mesh_terms: D064591:Allografts; D042683:Angiopoietin-1 / Q000235:genetics / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D064236:Cartilage Oligomeric Matrix Protein / Q000235:genetics / Q000494:pharmacology*; D005260:Female; D007249:Inflammation / Q000188:drug therapy; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism; D016381:Islets of Langerhans Transplantation / Q000295:instrumentation / Q000379:methods*; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects; D008297:Male; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D042683:Angiopoietin-1; D064236:Cartilage Oligomeric Matrix Protein; D011994:Recombinant Proteins; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4
keywords: Allograft; Angiopoietin-1; COMP-Ang1; Cartilage oligomeric matrix protein; Inflammation; Islet; Toll-like receptor 4; Transplantation