title: Activation energy associated with the electromigration of oligosaccharides through viscosity modifier and polymeric additive containing background electrolytes.
abstract: The activation energy related to the electromigration of oligosaccharides can be determined from their measured electrophoretic mobilities at different temperatures. The effects of a viscosity modifier (ethylene glycol) and a polymeric additive (linear polyacrylamide) on the electrophoretic mobility of linear sugar oligomers with α1-4 linked glucose units (maltooligosaccharides) were studied in CE using the activation energy concept. The electrophoretic separations of 8-aminopyrene-1,3,6-trisulfonate-labeled maltooligosaccharides were monitored by LIF detection in the temperature range of 20-50°C, using either 0-60% ethylene glycol (viscosity modifier) or 0-3% linear polyacrylamide (polymeric additive) containing BGEs. Activation energy curves were constructed based on the slopes of the Arrhenius plots. With the use of linear polyacrylamide additive, solute size-dependent activation energy variations were found for the maltooligosaccharides with polymerization degrees below and above maltoheptaose (DP 7), probably due to molecular conformation changes and possible matrix interaction effects.
authors.affiliation: Horváth Csaba Laboratory of Bioseparation Sciences, Regional Centre for Molecular Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Márta
authors.initials: M
authors.lastname: Kerékgyártó
authors.affiliation: MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Járvás
authors.affiliation: Department of Colloid and Environmental Chemistry, Faculty of Sciences and Technology, University of Debrecen, Debrecen, Hungary.
authors.forename: Levente
authors.initials: L
authors.lastname: Novák
authors.affiliation: Horváth Csaba Laboratory of Bioseparation Sciences, Regional Centre for Molecular Medicine, University of Debrecen, Debrecen, Hungary.|MTA-PE Translational Glycomics Research Group, University of Pannonia, Veszprem, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Guttman
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008956:Models, Chemical; D009844:Oligosaccharides / Q000737:chemistry*; D014783:Viscosity; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D004573:Electrolytes; D009844:Oligosaccharides; D000099166:Polyacrylamides
keywords: Activation energy; Capillary electrophoresis; Polyacrylamide; Viscosity