title: Amperometric Quantification of S-Nitrosoglutathione Using Gold Nanoparticles: A Step toward Determination of S-Nitrosothiols in Plasma.
abstract: S-Nitrosothiols (RSNOs) are carriers of nitric oxide (NO) and have important biological activities. We propose here the use of gold nanoparticles (AuNPs) and NO-selective amperometric microsensor for the detection and quantification of S-nitrosoglutathione (GSNO) as a step toward the determination of plasma RSNOs. AuNPs were used to decompose RSNOs with the quantitative release of free NO which was selectively detected with a NO microsensor. The optimal [GSNO]/[AuNPs] ratio was determined, corresponding to an excess of AuNP surface relative to the molar GSNO amount. Moreover, the influence of free plasma thiols on this method was investigated and a protocol based on the blocking of free thiols with iodoacetic acid, forming the carboxymethyl derivative of the cysteine residues, is proposed.
authors.affiliation: Institute of Chemistry, University of Campinas , UNICAMP, Campinas, São Paulo, 13083-970, Brazil.|Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), 75005 Paris, France.|INSERM, UTCBS, 75005, Paris, France.|CNRS, UTCBS UMR 8258, 75005 Paris, France.|Université Paris Descartes , Sorbonne Paris Cité, UTCBS, 75006 Paris, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Baldim
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), 75005 Paris, France.|INSERM, UTCBS, 75005, Paris, France.|CNRS, UTCBS UMR 8258, 75005 Paris, France.|Université Paris Descartes , Sorbonne Paris Cité, UTCBS, 75006 Paris, France.
authors.forename: Abdulghani
authors.initials: A
authors.lastname: Ismail
authors.affiliation: Institute of Chemistry, University of Campinas , UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Taladriz-Blanco
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), 75005 Paris, France.|INSERM, UTCBS, 75005, Paris, France.|CNRS, UTCBS UMR 8258, 75005 Paris, France.|Université Paris Descartes , Sorbonne Paris Cité, UTCBS, 75006 Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Griveau
authors.affiliation: Institute of Chemistry, University of Campinas , UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
authors.forename: Marcelo Ganzarolli
authors.initials: MG
authors.lastname: de Oliveira
authors.affiliation: Chimie ParisTech, PSL Research University, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), 75005 Paris, France.|INSERM, UTCBS, 75005, Paris, France.|CNRS, UTCBS UMR 8258, 75005 Paris, France.|Université Paris Descartes , Sorbonne Paris Cité, UTCBS, 75006 Paris, France.
authors.forename: Fethi
authors.initials: F
authors.lastname: Bedioui
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles; D026422:S-Nitrosoglutathione / Q000032:analysis*; D026403:S-Nitrosothiols / Q000097:blood*
chemical_list: D026403:S-Nitrosothiols; D026422:S-Nitrosoglutathione; D006046:Gold