title: Detection of Talaromyces marneffei from Fresh Tissue of an Inhalational Murine Pulmonary Model Using Nested PCR.
abstract: Penicilliosis marneffei, often consecutive to the aspiration of Talaromyces marneffei (Penicillium marneffei), continues to be one of the significant causes of morbidity and mortality in immunocompromised patients in endemic regions such as Southeast Asia. Improving the accuracy of diagnosing this disease would aid in reducing the mortality of associated infections. In this study, we developed a stable and reproducible murine pulmonary model that mimics human penicilliosis marneffei using a nebulizer to deliver Talaromyces marneffei (SUMS0152) conidia to the lungs of BALB/c nude mice housed in exposure chamber. Using this model, we further revealed that nested PCR was sensitive and specific for detecting Talaromyces marneffei in bronchoalveolar lavage fluid and fresh tissues. This inhalation model may provide a more representative analysis tool for studying the development of penicilliosis marneffei, in addition to revealing that nested PCR has a predictive value in reflecting pulmonary infection.
authors.affiliation: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Dermatology, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Yi
authors.affiliation: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: He
authors.affiliation: Division of Infectious Disease, Rhode Island Hospital, Waren Alpert Medical School of Brown University, Providence, Rhode Island, United States of America.
authors.forename: Eleftherios
authors.initials: E
authors.lastname: Mylonakis
authors.affiliation: Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Xi
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D008168:Lung / Q000382:microbiology* / Q000473:pathology*; D051379:Mice; D050296:Microbial Viability; D009181:Mycoses / Q000382:microbiology / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D032901:Talaromyces / Q000302:isolation & purification*
chemical_list: D000336:Aerosols