title: MICROBIOCENOSIS IN INFLAMMATORY PROCESSES OF MARGINAL GUM AMONG CHILDREN.
abstract: BACKGROUND
  The development of gingivitis among children remains actual problem in modern dentistry due to high prevalence, disorders of dental-alveolar functions and development of parodontium diseases at the older age. The microorganisms occupying dental chamfer play the leading role in parodontium tissues damage.
  
  
  OBJECTIVE
  Research of microbial spectrum of dental chamfer and assessment of etiology significances of the revealed microorganisms in gingivitis among children of school age.
  
  
  METHODS
  Bacteriological research of dental chamfer in 30 children of secondary school age with gingivitis of slight and severe level. Inoculations have been done on the chocolate agar incubated in air-locked containers with gas generators for anaerobic atmosphere "GENbag Anaer" and inoculations on bloody gemagar--with gas generators for microaerophiles "GENbag Microaer" (bioMerieux) within 24-48 hours. Infections were precipitated out on the Saburo medium. Identification of discharged cultures was carried out on the microbialogic evaluator "Vitek II Compact" (bioMerieux).
  
  
  RESULTS
  Microflora of dental chamfer in the examined children has been presented by three groups of microorganisms: gram-positive cocci (63.3%), gram-negative nonfermentative bacteriums (63.3%) and yeast Candida fungus (30%). Among gram-postitive cocci representatives of normal microflora of oral cavity Streptococcus oralis (26.6%) were dominated. Neisseria (N. sicca, cinerea et animaloris) were dominated most often in the group of gram-negative nonfermentative bacteriums (23.3% of children), yeast Candida fungus have been presented by two kinds--C. dubliensis (at 20% of children) and C. albicans (at 10%). The mean content of all discharged microorganisms has compounded 5,7 Ig KOE that supposes their etiology role in development of marginal gum inflammation.
  
  
  CONCLUSIONS
  Received results showed us some mechanisms of development of inflammatory diseases of parodontium tissues which will allow us to develop new approaches of treatment and gingivitis prevention among children of school age.
authors.forename: Y A
authors.initials: YA
authors.lastname: Akhremenko
authors.forename: A S
authors.initials: AS
authors.lastname: Cheremkina
authors.forename: L A
authors.initials: LA
authors.lastname: Tarasova
authors.forename: I D
authors.initials: ID
authors.lastname: Ushnitsky
mesh_terms: D000293:Adolescent; D002176:Candida albicans / Q000302:isolation & purification*; D002648:Child; D015169:Colony Count, Microbial; D003774:Dental Plaque Index; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D005891:Gingivitis / Q000209:etiology*; D006088:Gram-Negative Aerobic Bacteria / Q000302:isolation & purification*; D006095:Gram-Positive Cocci / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D010512:Periodontal Index