title: Responses in Micro-Mineral Metabolism in Rainbow Trout to Change in Dietary Ingredient Composition and Inclusion of a Micro-Mineral Premix.
abstract: Responses in micro-mineral metabolism to changes in dietary ingredient composition and inclusion of a micro-mineral premix (Fe, Cu, Mn, Zn and Se) were studied in rainbow trout. In a 2 x 2 factorial design, triplicate groups of rainbow trout (initial weight: 20 g) were fed over 12 weeks at 17°C a fishmeal-based diet (M) or a plant-ingredient based diet (V), with or without inclusion of a mineral premix. Trout fed the V vs. M diet had lower feed intake, growth, hepato-somatic index, apparent availability coefficient (AAC) of Fe, Cu, Mn and Zn and also lower whole body Se and Zn concentration, whereas whole body Fe and Cu and plasma Fe concentrations were higher. Feeding the V diet increased intestinal ferric reductase activity; at transcriptional level, hepatic hepcidin expression was down-regulated and ferroportin 1 was up-regulated. Transcription of intestinal Cu-transporting ATPases and hepatic copper transporter1 were higher in V0 compared to other groups. Among the hepatic metalo-enzyme activities assayed, only Se-dependent glutathione peroxidase was affected, being lower in V fed fish. Premix inclusion reduced the AAC of Fe, Cu and Zn; increased the whole body concentration of all micro- minerals; up-regulated hepatic hepcidin and down-regulated intestinal ferroportin 1 transcription; and reduced the transcription of Cu-transporting ATPases in the intestine. Overall, the regulation of micro-mineral metabolism in rainbow trout, especially Fe and Cu, was affected both by a change in ingredient composition and micro-mineral premix inclusion.
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR1067, Nutrition, Metabolism and Aquaculture (NuMeA), Aquapôle INRA, 64310, Saint-Pée-sur-Nivelle, France.|AgroParisTech, Paris Institute of Technology for Life, Food and Environmental Sciences, 16 rue Claude Bernard, 75231, Paris, Cedex 5, France.|Aquaculture and Fisheries Group, Wageningen Institute of Animal Sceinces (WIAS), Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Antony Jesu Prabhu
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR1067, Nutrition, Metabolism and Aquaculture (NuMeA), Aquapôle INRA, 64310, Saint-Pée-sur-Nivelle, France.
authors.forename: Inge
authors.initials: I
authors.lastname: Geurden
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR1067, Nutrition, Metabolism and Aquaculture (NuMeA), Aquapôle INRA, 64310, Saint-Pée-sur-Nivelle, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Fontagné-Dicharry
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR1067, Nutrition, Metabolism and Aquaculture (NuMeA), Aquapôle INRA, 64310, Saint-Pée-sur-Nivelle, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Veron
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR1067, Nutrition, Metabolism and Aquaculture (NuMeA), Aquapôle INRA, 64310, Saint-Pée-sur-Nivelle, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Larroquet
authors.affiliation: AgroParisTech, Paris Institute of Technology for Life, Food and Environmental Sciences, 16 rue Claude Bernard, 75231, Paris, Cedex 5, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Mariojouls
authors.affiliation: Aquaculture and Fisheries Group, Wageningen Institute of Animal Sceinces (WIAS), Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.
authors.forename: Johan W
authors.initials: JW
authors.lastname: Schrama
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR1067, Nutrition, Metabolism and Aquaculture (NuMeA), Aquapôle INRA, 64310, Saint-Pée-sur-Nivelle, France.
authors.forename: Sadasivam J
authors.initials: SJ
authors.lastname: Kaushik
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015870:Gene Expression; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008903:Minerals / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010445:Peptide Elongation Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D008903:Minerals; D010445:Peptide Elongation Factors; D007501:Iron