title: Optimization of Biomass and 5-Aminolevulinic Acid Production by Rhodobacter sphaeroides ATCC17023 via Response Surface Methodology.
abstract: Microbial 5-aminolevulinic acid (ALA) produced from wastewater is considered as potential renewable energy. However, many hurdles are needed to be overcome such as the regulation of key influencing factors on ALA yield. Biomass and ALA production by Rhodobacter sphaeroides was optimized using response surface methodology. The culturing medium was artificial volatile fatty acids wastewater. Three additives were optimized, namely succinate and glycine that are precursors of ALA biosynthesis, and D-glucose that is an inhibitor of ALA dehydratase. The optimal conditions were achieved by analyzing the response surface plots. Statistical analysis showed that succinate at 8.56 mmol/L, glycine at 5.06 mmol/L, and D-glucose at 7.82 mmol/L were the best conditions. Under these optimal conditions, the highest biomass production and ALA yield of 3.55 g/L and 5.49 mg/g-biomass were achieved. Subsequent verification experiments at optimal values had the maximum biomass production of 3.41 ± 0.002 g/L and ALA yield of 5.78 ± 0.08 mg/g-biomass.
authors.affiliation: School of Environmental and Municipal Engineering, North China University of Water Resources and Electric Power, Zhengzhou, 450000, China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Environment and Resource, Renmin University of China, Beijing, 100872, China. zgm@ruc.edu.cn.|School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China. zgm@ruc.edu.cn.
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China.
authors.forename: Jianzheng
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China.
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Huanghe Road 73, Harbin, 150090, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000622:Aminolevulinic Acid / Q000737:chemistry / Q000378:metabolism*; D018533:Biomass; D005232:Fatty Acids, Volatile / Q000737:chemistry / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D000623:Porphobilinogen Synthase / Q000737:chemistry; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D013499:Surface Properties; D062065:Wastewater / Q000737:chemistry
chemical_list: D000622:Aminolevulinic Acid; D005232:Fatty Acids, Volatile; D000623:Porphobilinogen Synthase; D062065:Wastewater
keywords: 5-Aminolevulinic acid yield; Biomass; Influencing factor; Response surface methodology; Rhodobacter sphaeroides; Wastewater