title: Cyanobacterial chassis engineering for enhancing production of biofuels and chemicals.
abstract: To reduce dependence on fossil fuels and curb greenhouse effect, cyanobacteria have emerged as an important chassis candidate for producing biofuels and chemicals due to their capability to directly utilize sunlight and CO2 as the sole energy and carbon sources, respectively. Recent progresses in developing and applying various synthetic biology tools have led to the successful constructions of novel pathways of several dozen green fuels and chemicals utilizing cyanobacterial chassis. Meanwhile, it is increasingly recognized that in order to enhance productivity of the synthetic cyanobacterial systems, optimizing and engineering more robust and high-efficient cyanobacterial chassis should not be omitted. In recent years, numerous research studies have been conducted to enhance production of green fuels and chemicals through cyanobacterial chassis modifications involving photosynthesis, CO2 uptake and fixation, products exporting, tolerance, and cellular regulation. In this article, we critically reviewed recent progresses and universal strategies in cyanobacterial chassis engineering to make it more robust and effective for bio-chemicals production.
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, People's Republic of China.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Gao
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, People's Republic of China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Pei
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300072, People's Republic of China. lchen@tju.edu.cn.|Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China. lchen@tju.edu.cn.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, People's Republic of China. lchen@tju.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300072, People's Republic of China.|Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China.|SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, People's Republic of China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D056804:Biofuels / Q000032:analysis*; D000458:Cyanobacteria / Q000235:genetics* / Q000378:metabolism*; D005818:Genetic Engineering; D007218:Industrial Microbiology; D010788:Photosynthesis
chemical_list: D056804:Biofuels
keywords: Biofuels and chemicals; Chassis engineering; Cyanobacteria; Synthetic biology