title: Hydrogen sulfide improves neural function in rats following cardiopulmonary resuscitation.
abstract: The alleviation of brain injury is a key issue following cardiopulmonary resuscitation (CPR). Hydrogen sulfide (H2S) is hypothesized to be involved in the pathophysiological process of ischemia-reperfusion injury, and exerts a protective effect on neurons. The aim of the present study was to investigate the effects of H2S on neural functions following cardiac arrest (CA) in rats. A total of 60 rats were allocated at random into three groups. CA was induced to establish the model and CPR was performed after 6 min. Subsequently, sodium hydrosulfide (NaHS), hydroxylamine or saline was administered to the rats. Serum levels of H2S, neuron-specific enolase (NSE) and S100β were determined following CPR. In addition, neurological deficit scoring (NDS), the beam walking test (BWT), prehensile traction test and Morris water maze experiment were conducted. Neuronal apoptosis rates were detected in the hippocampal region following sacrifice. After CPR, as the H2S levels increased or decreased, the serum NSE and S100β concentrations decreased or increased, respectively (P<0.0w. The NDS results of the NaHS group were improved compared with those of the hydroxylamine group at 24 h after CPR (P<0.05). In the Morris water maze experiment, BWT and prehensile traction test the animals in the NaHS group performed best and rats in the hydroxylamine group performed worst. At day 7, the apoptotic index and the expression of caspase-3 were reduced in the hippocampal CA1 region, while the expression of Bcl-2 increased in the NaHS group; and results of the hydroxylamine group were in contrast. Therefore, the results of the present study indicate that H2S is able to improve neural function in rats following CPR.
authors.affiliation: Department of Emergency and Critical Care Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Ji-Yan
authors.initials: JY
authors.lastname: Lin
authors.affiliation: Department of Emergency and Critical Care Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Min-Wei
authors.initials: MW
authors.lastname: Zhang
authors.affiliation: Department of Emergency and Critical Care Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Jin-Gao
authors.initials: JG
authors.lastname: Wang
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Sun-Yat Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Sun-Yat Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Wei
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Sun-Yat Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.affiliation: Key Laboratory on Assisted Circulation, Ministry of Health, Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Dai
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Sun-Yat Sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Liao
keywords: apoptosis; brain injury; cardiopulmonary resuscitation; hydrogen sulfide; neurological behavior