title: Effect of bone marrow mesenchymal stem cells transplantation on the serum and liver HMGB1 expression in rats with acute liver failure.
abstract: OBJECTIVE
  This study aimed to investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of high mobility group box 1 protein (HMGB1) in the serum and liver of rats with acute liver failure (ALF).
  
  
  METHODS
  Healthy male SD rats were randomly divided into control group, ALF group and BMSCs group. ALF was induced by intraperitoneal injection of 900 mg/kg D-GalN and 10 μg/kg LPS. In BMSCs group, rats received BMSCs (1.0×10(7)) transplantation via the tail vein at 2 h after ALF induction.
  
  
  RESULTS
  Intraperitoneal injection of 900 mg/kg D-GalN and 10 μg/kg LPS was able to induce ALF in rats. In ALF group, serum ALT and AST increased gradually over time. At 72 h, the serum ALT and AST in BMSCs group were significantly different from those in ALF group. HMGB1 expression in the serum and liver remained at a low level at any time point in control group, but increased significantly in ALF group and BMSCs group. The serum and liver HMGB1 expression increased progressively in ALF group, but reduced gradually in BMSCs group. Significant difference in serum and liver HMGB1 expression was observed between ALF group and BMSCs group at 24 h and 72 h. In addition, there was marked difference in the survival rate among three groups at 24 h (χ (2) =21.098, P<0.01).
  
  
  CONCLUSIONS
  BMSCs transplantation is able to improve the liver function and liver pathology in ALF rats and decrease the serum and liver HMGB1.
authors.affiliation: Department of Gastroenterology, Third People's Hospital of Yunnan Province Kunming 650011, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Gastroenterology, Third People's Hospital of Yunnan Province Kunming 650011, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Center for Liver Diseases, Second Affiliated Hospital of Kunming Medical University Kunming 650021, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Center for Liver Diseases, Second Affiliated Hospital of Kunming Medical University Kunming 650021, China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Hepatobiliary Surgery, Third People's Hospital of Yunnan Province Kunming 650011, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Shao
authors.affiliation: Department of Scientific Research and Education, Third People's Hospital of Yunnan Province Kunming 650011, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Guo
authors.affiliation: Yunnan University Kunming 650000, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D016026:Bone Marrow Transplantation; D004195:Disease Models, Animal; D015536:Down-Regulation; D005688:Galactosamine; D024243:HMGB1 Protein / Q000097:blood* / Q000235:genetics; D007150:Immunohistochemistry; D008070:Lipopolysaccharides; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D017114:Liver Failure, Acute / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D015415:Biomarkers; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D005688:Galactosamine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Bone marrow mesenchymal stem cells; acute liver failure; high mobility group box 1 protein; rat; transplantation