title: [Expression, roles and therapy target values of CD24 in oral squamous cell carcinoma].
abstract: OBJECTIVE
  To determine the expression profile and potential roles of CD24 in oral squamous cell carcinoma and explore the values of CD24 function as a potential target of clinical therapy.
  
  
  METHODS
  Semi-quantitative immunohistochemistry was used to construct the expression profile of CD24 in 78 human oral tissues and 59 Hamster buccal pouch tissues. Real-time RT-PCR and Western blot were used to analyze the CD24 expression levels in oral DOK4 cells, oral cancer CAL-27 and WSU-HN6 cells. Then these two cancer cell lines were selected to evaluate the effect of all-trans retinoic acid (ATRA) and CD24 antibody on CD24 expression, and the proliferation and tumorsphere formation capacity of these two cell lines.
  
  
  RESULTS
  CD24 expression was found significantly elevated in both human and animal tissues compared with normal and benign tissues (P<0.05), as well as in oral cancer CAL-27 and WSU-HN6 cells compared with DOK cells (P<0.05). CAL-27 and WSU-HN6 cells possess increased proliferative and specific tumorsphere formation capability compared with DOK cells (P<0.05). Both ATRA and CD24 antibody were able to effectively inhibit the proliferation and tumorsphere formation of CAL-27 and WSU-HN6 cells (P<0.05). Among them ATRA at least involved partially in the proliferation by down-regulating the CD24 expression (P<0.05), while CD24 antibody blocking had no effect on the CD24 expression.
  
  
  CONCLUSIONS
  CD24 was upregulated in oral cancer and functioned as a potential factor that promoted the proliferation and tumorsphere formation of CAL-27 and WSU-HN6 cells. Both ATRA and CD24 antibody might effectively inhibit the proliferation and tumorsphere formation of CAL-27 and WSU-HN6 cells and function as a potential therapy target.
authors.affiliation: Department of Stomatology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Mo
authors.affiliation: Department of Stomatology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Cheng-zhi
authors.initials: CZ
authors.lastname: Gao
authors.affiliation: Department of Chinese Medicine, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Shao-jie
authors.initials: SJ
authors.lastname: Wang
authors.affiliation: Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Dong
authors.affiliation: Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Yu
mesh_terms: D000818:Animals; D051927:CD24 Antigen / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D006224:Cricetinae; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D051927:CD24 Antigen; C497889:CD24 protein, human; D014212:Tretinoin