title: Lattice filter for processing image data of three-dimensional protein nanocrystals.
abstract: When 300 kV cryo-EM images at Scherzer focus are acquired from ∼ 100 nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devoid of any contrast. Here, it is reported how this lattice information can be enhanced by means of a wave finder in combination with Wiener-type maximum-likelihood filtering. This procedure paves the way towards full three-dimensional structure determination at high resolution for protein crystals.
authors.affiliation: Biophysical Structural Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: van Genderen
authors.affiliation: Biophysical Structural Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: Y W
authors.initials: YW
authors.lastname: Li
authors.affiliation: Biophysical Structural Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: Nederlof
authors.affiliation: Biophysical Structural Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: J P
authors.initials: JP
authors.lastname: Abrahams
mesh_terms: D000465:Algorithms; D000818:Animals; D020285:Cryoelectron Microscopy / Q000379:methods*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D016013:Likelihood Functions; D009113:Muramidase / Q000737:chemistry / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure*; D011506:Proteins / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D011506:Proteins; D009113:Muramidase
keywords: cryo-EM; lattice filter; nanocrystals