title: Radical Pair-Driven Luminescence of Quantum Dots for Specific Detection of Peroxynitrite in Living Cells.
abstract: There is currently great interest in developing chemiluminescence (CL) probes that can selectively detect peroxynitrite (ONOO(-)) in living cells. In comparison with other reactive oxygen species (ROS), ONOO(-) can spontaneously decompose into a series of radicals. Notably, the interaction of quantum dots (QDs) with oxidizing/reducing ROS radicals can generate a strong CL emission by electron-transfer annihilation. Herein, we report a novel CL probe that affords the ability to distinguish ONOO(-) from other ROS in living cells. ONOO(-) can activate luminescence of QDs in the absence of excitation source, effectively avoiding background noise and scattering of light from biological matrixes produced by in situ excitation; however, there is no response to other ROS including (1)O2, H2O2, (•)OH, O2(•-), and ClO(-). The outstanding selectivity of the present CL probe leads us to detect the exogenous release of ONOO(-) from 3-morpholinosydnonimine (SIN-1) in living cells. These results suggest that this present probe-based CL provides a promising platform for highly selective and sensitive detection of ONOO(-) in biological systems.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Sui
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
mesh_terms: D002104:Cadmium / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D049449:Luminescence; D010084:Oxidation-Reduction; D030421:Peroxynitrous Acid / Q000032:analysis*; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry
chemical_list: D002104:Cadmium; D030421:Peroxynitrous Acid; D013691:Tellurium