title: NMR assignments of mitochondrial cyclophilin Cpr3 from Saccharomyces cerevisiae.
abstract: Cyclophilins regulate protein folding, transport and signalling through catalysis of proline isomerization, and are ubiquitously expressed in both prokaryotes and eukaryotes. Cpr3 is the yeast mitochondrial cyclophilin and it is structurally and biophysically uncharacterized so far. Yeast cyclophilin gene cpr3 is essential for the lactate metabolism. Here, we report (1)H, (13)C, and (15)N chemical shift assignments of Cpr3 protein determined by various 2D and 3D heteronuclear NMR experiments at pH 6.5, and temperature 298 K.
authors.affiliation: UM-DAE-Centre for Excellence in Basic Sciences, University of Mumbai, Kalina Campus, Mumbai, 400098, India.
authors.forename: Vaibhav Kumar
authors.initials: VK
authors.lastname: Shukla
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Mumbai, 400076, India.
authors.forename: Jai Shankar
authors.initials: JS
authors.lastname: Singh
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Mumbai, 400076, India.
authors.forename: Dipesh
authors.initials: D
authors.lastname: Trivedi
authors.affiliation: UM-DAE-Centre for Excellence in Basic Sciences, University of Mumbai, Kalina Campus, Mumbai, 400098, India. rvhosur53@gmail.com.
authors.forename: Ramakrishna V
authors.initials: RV
authors.lastname: Hosur
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Mumbai, 400076, India. ashutoshk@iitb.ac.in.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Kumar
mesh_terms: D021983:Cyclophilins / Q000737:chemistry*; D008928:Mitochondria; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D021983:Cyclophilins
keywords: Cpr3; Cyclophilins; NMR; Resonance assignment