title: High-throughput fluorescence microscopic analysis of protein abundance and localization in budding yeast.
abstract: Proteins directly carry out and regulate cellular functions. As a result, changes in protein levels within a cell directly influence cellular processes. Similarly, it is intuitive that the intracellular localization of proteins is a key component of their functionality. Optimal activity is achieved by a combination of protein concentration, co-compartmentalization with substrates, co-factors and regulators and sequestration from deleterious locales. The proteome within a cell is highly dynamic and changes in response to different environmental conditions. High-throughput microscopic analysis in the budding yeast Saccharomyces cerevisiae has afforded proteome-wide views of protein organization in living cells, and of how protein abundance and location is regulated and remodeled in response to stress.
authors.affiliation: a Department of Biochemistry and Donnelly Centre , University of Toronto , Toronto , Ontario , Canada.
authors.forename: Nikko P
authors.initials: NP
authors.lastname: Torres
authors.affiliation: a Department of Biochemistry and Donnelly Centre , University of Toronto , Toronto , Ontario , Canada.
authors.forename: Brandon
authors.initials: B
authors.lastname: Ho
authors.affiliation: a Department of Biochemistry and Donnelly Centre , University of Toronto , Toronto , Ontario , Canada.
authors.forename: Grant W
authors.initials: GW
authors.lastname: Brown
mesh_terms: D057166:High-Throughput Screening Assays; D008856:Microscopy, Fluorescence / Q000379:methods*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: Fluorescence microscopy; genomics; protein abundance; protein localization; quantitative cell biology; yeast