title: Unsuccessful Detection of Plant MicroRNAs in Beer, Extra Virgin Olive Oil and Human Plasma After an Acute Ingestion of Extra Virgin Olive Oil.
abstract: The recent description of the presence of exogenous plant microRNAs from rice in human plasma had profound implications for the interpretation of microRNAs function in human health. If validated, these results suggest that food should not be considered only as a macronutrient and micronutrient supplier but it could also be a way of genomic interchange between kingdoms. Subsequently, several studies have tried to replicate these results in rice and other plant foods and most of them have failed to find plant microRNAs in human plasma. In this scenario, we aimed to detect plant microRNAs in beer and extra virgin olive oil (EVOO)--two plant-derived liquid products frequently consumed in Spain--as well as in human plasma after an acute ingestion of EVOO. Our hypothesis was that microRNAs present in beer and EVOO raw material could survive manufacturing processes, be part of these liquid products, be absorbed by human gut and circulate in human plasma. To test this hypothesis, we first optimized the microRNA extraction protocol to extract microRNAs from beer and EVOO, and then tried to detect microRNAs in those samples and in plasma samples of healthy volunteers after an acute ingestion of EVOO.
authors.affiliation: Genómica Nutricional de la Enfermedad Cardiovascular y la Obesidad. Fundación IMDEA Alimentación, CEI UAM + CSIC, 28049, Madrid, Spain.
authors.forename: Victor
authors.initials: V
authors.lastname: Micó
authors.affiliation: Genómica Nutricional de la Enfermedad Cardiovascular y la Obesidad. Fundación IMDEA Alimentación, CEI UAM + CSIC, 28049, Madrid, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Martín
authors.affiliation: Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRyCIS), E-28034, Madrid, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Lasunción
authors.affiliation: Genómica Nutricional de la Enfermedad Cardiovascular y la Obesidad. Fundación IMDEA Alimentación, CEI UAM + CSIC, 28049, Madrid, Spain.|Nutrition and Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University, Boston, Massachusetts, MA, -02111, USA.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovás
authors.affiliation: Genómica Nutricional de la Enfermedad Cardiovascular y la Obesidad. Fundación IMDEA Alimentación, CEI UAM + CSIC, 28049, Madrid, Spain. lidia.daimiel@imdea.org.
authors.forename: Lidia
authors.identifier: http://orcid.org/0000-0001-9898-6629
authors.initials: L
authors.lastname: Daimiel
mesh_terms: D000818:Animals; D001515:Beer / Q000032:analysis*; D004435:Eating; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000097:blood; D008892:Milk / Q000737:chemistry; D000069463:Olive Oil / Q000032:analysis*; D012275:Oryza / Q000235:genetics*; D018749:RNA, Plant / Q000032:analysis / Q000097:blood; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs; D000069463:Olive Oil; D018749:RNA, Plant
keywords: Beer; Extra virgin olive oil; Next generation sequencing; Plant microRNAs