title: Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles.
abstract: A novel magnetic fluorescent encoded nanoimmunoassay system for multicomponent detection and separation of the subtrace pathogenic DNA (hepatitis B virus surface gene, HBV; hepatitis A virus poly the protein gene, HAV) was established based on new type of magnetic fluorescent encoded nanoparticles and sandwich immunoassay principle. This method combines multifunctional nanoparticles, immunoassay technique, fluorescence labeling, and magnetic separation of multicomponent technology. It has many advantages such as high sensitivity, low detection limit, easy operation, and great potential for development. The results of this work show that, based on nanoimmunoassay system, it could quantitatively detect the multicomponent trace pathogenic HAV and HBV DNA, as well as detection limit up to 0.1 pM and 0.12 pM. Furthermore, with the improvement of the performances of magnetic fluorescent encoded nanoparticles, the sensitivity will be further improved. In this experiment, a new nanoimmunoassay system based on magnetic fluorescent encoded nanoparticles was established, which will provide a new way for the immunoassay and separation of multicomponent biomolecules.
authors.affiliation: Department of Chemistry, College of Pharmacy, Liaoning Medical University, Jinzhou 121001, China.
authors.forename: Yaxu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau.
authors.forename: Xuanjun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, College of Pharmacy, Liaoning Medical University, Jinzhou 121001, China.
authors.forename: E
authors.initials: E
authors.lastname: Yifeng
authors.affiliation: Department of Chemistry, College of Pharmacy, Liaoning Medical University, Jinzhou 121001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Department of Chemistry, College of Pharmacy, Liaoning Medical University, Jinzhou 121001, China.
authors.forename: Guangkai
authors.initials: G
authors.lastname: Kuang
authors.affiliation: Department of Chemistry, College of Pharmacy, Liaoning Medical University, Jinzhou 121001, China.
authors.forename: Guannan
authors.initials: G
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005453:Fluorescence; D015684:Gene Products, pol / Q000235:genetics / Q000302:isolation & purification; D006506:Hepatitis A / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D006509:Hepatitis B / Q000175:diagnosis / Q000235:genetics / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D015684:Gene Products, pol; D006514:Hepatitis B Surface Antigens; D058185:Magnetite Nanoparticles