title: CnaA domains in bacterial pili are efficient dissipaters of large mechanical shocks.
abstract: Pathogenic bacteria adhere despite severe mechanical perturbations induced by the host, such as coughing. In Gram-positive bacteria, extracellular protein appendages termed pili are necessary for adherence under mechanical stress. However, little is known about the behavior of Gram-positive pili under force. Here, we demonstrate a mechanism by which Gram-positive pili are able to dissipate mechanical energy through mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops present in Ig-type CnaA domains. Using single-molecule force spectroscopy, we find that these loops of the pilus subunit SpaA of the SpaA-type pilus from Corynebacterium diphtheriae and FimA of the type 2 pilus from Actinomyces oris unfold and extend at forces that are the highest yet reported for globular proteins. Loop refolding is limited by the hydrophobic collapse of the polypeptide and occurs in milliseconds. Remarkably, both SpaA and FimA initially refold to mechanically weaker intermediates that recover strength with time or ligand binding. Based on the high force extensibility, CnaA-containing pili can dissipate ∼28-fold as much energy compared with their inextensible counterparts before reaching forces sufficient to cleave covalent bonds. We propose that efficient mechanical energy dissipation is key for sustained bacterial attachment against mechanical perturbations.
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027; dje2122@columbia.edu jorge.alegre@cnic.es jfernandez@columbia.edu.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Echelman
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027; Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 28029 Madrid, Spain; dje2122@columbia.edu jorge.alegre@cnic.es jfernandez@columbia.edu.
authors.forename: Jorge
authors.initials: J
authors.lastname: Alegre-Cebollada
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027;
authors.forename: Carmen L
authors.initials: CL
authors.lastname: Badilla
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, Houston, TX 77030.
authors.forename: Chungyu
authors.initials: C
authors.lastname: Chang
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, Houston, TX 77030.
authors.forename: Hung
authors.initials: H
authors.lastname: Ton-That
authors.affiliation: Department of Biological Sciences, Columbia University, New York, NY 10027; dje2122@columbia.edu jorge.alegre@cnic.es jfernandez@columbia.edu.
authors.forename: Julio M
authors.initials: JM
authors.lastname: Fernández
mesh_terms: D000190:Actinomyces / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D003353:Corynebacterium diphtheriae / Q000737:chemistry; D010861:Fimbriae, Bacterial / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins
keywords: Gram-positive pili; bacterial adhesion; isopeptide bond; mechanical stability; single-molecule force spectroscopy