title: Phylogenetic classification of Aureobasidium pullulans strains for production of feruloyl esterase.
abstract: OBJECTIVE
  The objective was to phylogenetically classify diverse strains of Aureobasidium pullulans and determine their production of feruloyl esterase.
  
  
  RESULTS
  Seventeen strains from the A. pullulans literature were phylogenetically classified. Phenotypic traits of color variation and endo-β-1,4-xylanase overproduction were associated with phylogenetic clade 10 and particularly clade 8. Literature strains used for pullulan production all belonged to clade 7. These strains and 36 previously classified strains were tested for feruloyl esterase production, which was found to be associated with phylogenetic clades 4, 11, and particularly clade 8. Clade 8 strains NRRL 58552 and NRRL 62041 produced the highest levels of feruloyl esterase among strains tested.
  
  
  CONCLUSIONS
  Production of both xylanase and feruloyl esterase are associated with A. pullulans strains in phylogenetic clade 8, which is thus a promising source of enzymes with potential biotechnological applications.
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA.
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Rich
authors.affiliation: School of Biological Sciences, Illinois State University, Normal, IL, USA.
authors.forename: Pennapa
authors.initials: P
authors.lastname: Manitchotpisit
authors.affiliation: Mycotoxins and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA.
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Peterson
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA.
authors.forename: Siqing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA. Tim.Leathers@ars.usda.gov.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Leathers
authors.affiliation: Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA.
authors.forename: Amber M
authors.initials: AM
authors.lastname: Anderson
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics; D014995:Xylosidases / Q000378:metabolism
chemical_list: D021903:DNA, Ribosomal Spacer; D014404:Tubulin; D012319:RNA Polymerase II; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D014995:Xylosidases
keywords: Aureobasidium pullulans; Color variant; Ferulic acid; Phylogeny; Pullulan