title: A microRNA from infectious spleen and kidney necrosis virus modulates expression of the virus-mock basement membrane component VP08R.
abstract: Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus, family Iridoviridae. Infection of ISKNV is characterized by a unique pathological phenomenon in that the infected cells are attached by lymphatic endothelial cells (LECs). ISKNV mediates the formation of a virus-mock basement membrane (VMBM) structure on the surface of infected cells to provide attaching sites for LECs. The viral protein VP08R is an important component of VMBM. In this study, a novel ISKNV-encoded microRNA, temporarily named ISKNV-miR-1, was identified. ISKNV-miR-1 is complementary to the VP08R-coding sequence and can modulate VP08R expression through reducing its mRNA level. This suggests that formation of VMBM may be under fine regulation by ISKNV.
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Muting
authors.initials: M
authors.lastname: Yan
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xia
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Weng
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China; School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China. Electronic address: lsshjg@mail.sysu.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China. Electronic address: xuxpeng@mail.sysu.edu.cn.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001485:Basement Membrane; D002448:Cell Adhesion; D004266:DNA Virus Infections / Q000473:pathology / Q000821:virology*; D042783:Endothelial Cells / Q000473:pathology / Q000821:virology; D004847:Epithelial Cells / Q000473:pathology / Q000821:virology; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D005399:Fishes; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D007497:Iridoviridae / Q000235:genetics* / Q000472:pathogenicity; D007668:Kidney / Q000473:pathology / Q000821:virology; D008196:Lymph / Q000166:cytology / Q000821:virology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000473:pathology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D014764:Viral Proteins
keywords: ISKNV; Iridovirus; Megalocytivirus; MicroRNA; VP08R; Virus-mock basement membrane