title: Cinnamaldehyde supplementation prevents fasting-induced hyperphagia, lipid accumulation, and inflammation in high-fat diet-fed mice.
abstract: Cinnamaldehyde, a bioactive component of cinnamon, is increasingly gaining interest for its preventive and therapeutic effects against metabolic complications like type-2 diabetes. This study is an attempt to understand the effect of cinnamaldehyde in high-fat diet (HFD)-associated increase in fasting-induced hyperphagia and related hormone levels, adipose tissue lipolysis and inflammation, and selected cecal microbial count in mice. Cinnamaldehyde, at 40 µM dose, prevented lipid accumulation and altered gene expression toward lipolytic phenotype in 3T3-L1 preadipocyte cell lines. In vivo, cinnamaldehyde coadministration prevented HFD-induced body weight gain, decreased fasting-induced hyperphagia, as well as circulating leptin and leptin/ghrelin ratio. In addition to that, cinnamaldehyde altered serum biochemical parameters related to lipolysis, that is, glycerol and free fatty acid levels. At transcriptional level, cinnamaldehyde increased anorectic gene expression in hypothalamus and lipolytic gene expression in visceral white adipose tissue. Furthermore, cinnamaldehyde also decreased serum IL-1β and inflammatory gene expression in visceral white adipose tissue. However, cinnamaldehyde did not modulate the population of selected gut microbial (Lactobacillus, Bifidibaceria, and Roseburia) count in cecal content. In conclusion, cinnamaldehyde increased adipose tissue lipolysis, decreased fasting-induced hyperphagia, normalized circulating levels of leptin/ghrelin ratio, and reduced inflammation in HFD-fed mice, which augurs well for its antiobesity role.
authors.affiliation: National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, India.|Department of Pharmacology, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh, India.
authors.forename: Pragyanshu
authors.initials: P
authors.lastname: Khare
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Punjab, India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Jagtap
authors.affiliation: National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, India.
authors.forename: Yachna
authors.initials: Y
authors.lastname: Jain
authors.affiliation: National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, India.
authors.forename: Ritesh K
authors.initials: RK
authors.lastname: Baboota
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Punjab, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Mangal
authors.affiliation: Department of Biotechnology, Government College for Girls-Sector 42, Chandigarh, India.
authors.forename: Ravneet K
authors.initials: RK
authors.lastname: Boparai
authors.affiliation: Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Punjab, India.
authors.forename: Kamlesh K
authors.initials: KK
authors.lastname: Bhutani
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), SAS Nagar, Punjab, India.
authors.forename: Shyam S
authors.initials: SS
authors.lastname: Sharma
authors.affiliation: Department of Pharmacology, Southern Illinois University-School of Medicine, Springfield, IL, USA.
authors.forename: Louis S
authors.initials: LS
authors.lastname: Premkumar
authors.affiliation: National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, India.
authors.forename: Kanthi K
authors.initials: KK
authors.lastname: Kondepudi
authors.affiliation: Department of Pharmacology, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh, India.
authors.forename: Kanwaljit
authors.initials: K
authors.lastname: Chopra
authors.affiliation: National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, India.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Bishnoi
mesh_terms: D041721:3T3-L1 Cells; D000171:Acrolein / Q000008:administration & dosage / Q000031:analogs & derivatives*; D000273:Adipose Tissue / Q000187:drug effects / Q000473:pathology; D000818:Animals; D059305:Diet, High-Fat; D019587:Dietary Supplements; D005215:Fasting / Q000009:adverse effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006963:Hyperphagia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007249:Inflammation / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D053583:Interleukin-1beta / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects; D008066:Lipolysis / Q000187:drug effects; D051379:Mice; D015430:Weight Gain / Q000187:drug effects
chemical_list: D053583:Interleukin-1beta; D000171:Acrolein; C012843:cinnamaldehyde
keywords: adipose tissue; cinnamaldehyde; diet-induced obesity; gut microbiota; hyperphagia ghrelin; leptin; lipolysis