title: A comparative analysis of different molecular targets using PCR for diagnosis of old world leishmaniasis.
abstract: The different sensitivity values were obtained in each study conducted for the diagnosis of leishmaniasis with the polymerase chain reaction (PCR). However, a standardized PCR target for the diagnosis of leishmaniasis does not exist. The aim of the current study, the most ideal PCR target was determined for diagnosis of leishmaniasis. A total of 72 smear and 48 bone marrow samples were analyzed with six different molecular targets to determine their potential as a tool for the specific molecular diagnosis of leishmaniasis using PCR. The positivity-negativity value and the sensitivity-specificity of each PCR targets were calculated. The positivity value of PCR targets were sequenced in different levels in the diagnosis of leishmaniasis from highest to lowest in the order of kDNA-PCR > SSU rRNA-PCR > ITS2-PCR > ITS1-PCR > ME-PCR > HSP70-PCR. The sensitivities of PCR targets except ITS1-PCR, ME-PCR and HSP70-PCR were found to be 100% in cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) cases as compared to microscopic examination accepted as a gold standard. The sensitivities of ITS1-PCR, ME-PCR and HSP70-PCR were found 96.6%, 90.0% and 86.6%, respectively, in CL-cases. In addition, the sensitivities of ITS1-PCR, ME-PCR and HSP70-PCR were found 90.0%, 70.0% and 60.0%, respectively, in VL-cases. The kDNA genomic region was the most sensitive for routine diagnosis of leishmaniasis. ITS1-PCR restriction fragment length polymorphism, the alternative method for the identification of Old World Leishmania species, did not require culturing of the parasites.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Cukurova, 01130, Balcali, Saricam, Adana, Turkey. Electronic address: koltas@cu.edu.tr.
authors.forename: Ismail S
authors.initials: IS
authors.lastname: Koltas
authors.affiliation: Emine-Bahaeddin Nakıboglu Faculty of Medicine, Department of Medical Microbiology, Zirve University, Gaziantep, Turkey. Electronic address: eroglufadime@hotmail.com.
authors.forename: Fadime
authors.initials: F
authors.lastname: Eroglu
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Akdeniz, 07070, Konyaalti, Antalya, Turkey. Electronic address: sonuzun@yahoo.com.
authors.forename: Soner
authors.initials: S
authors.lastname: Uzun
authors.affiliation: Department of Pediatrics, Faculty of Medicine, University of Cukurova, 01130, Balcali, Saricam, Adana, Turkey. Electronic address: dalabaz@yahoo.com.
authors.forename: Derya
authors.initials: D
authors.lastname: Alabaz
mesh_terms: D001853:Bone Marrow / Q000469:parasitology*; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D016054:DNA, Protozoan
keywords: Genomic region; Leishmaniasis; Polymerase chain reaction; Restriction fragment length polymorphism