title: Experimental study on apoptosis of TNFR1 receptor pro-endothelial progenitor cells activated by high glucose induced oxidative stress.
abstract: OBJECTIVE
  To investigate whether high glucose in vitro activating TNFR1 and further promote rat marrow endothelial progenitor cells (EPCs) apoptosis.
  
  
  METHODS
  Rat morrow endothelial progenitor cells were cultured and identified by Confocal Microscopy; then were treated with high glucose (5.5, 15, 30, 60 mmol/L), mannitol (15, 30, 60, 90 mmol/L), high glucose + Tempol and high glucose+ MAB430. Apoptosis rate of the above cells were detected by flow cytometry. ROS and MDA level and anti-O2- were detected by colorimetric technique; the expression level of TNFR1 induced signal pathway related proteins were detected by Western blotting.
  
  
  RESULTS
  High glucose can induce endothelial progenitor cells apoptosis, which is mostly in the later stage (72 h-96 h) instead of the earlier stage (24 h-48 h); high glucose can also induce oxidative stress reaction and the produces ROS and MDA increase significantly in the later stage (after 72 h), but anti-O2- decrease significantly. TNF apoptosis signal pathway related protein expression level not increase in the earlier stage (before 24 h) but increase significantly in the later stage (after 72 h). Tempol and MAB430 down-regulate TNF apoptosis signal pathway related protein expression and reduce EPCs apoptosis.
  
  
  CONCLUSIONS
  High glucose activates the TNFR1 of TPCs through oxidative stress reaction and further induces cell apoptosis.
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College Luzhou Sichuan, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College LuzhouSichuan, China; First People's Hospital of Liangshan Yi Autonomous PrefectureLiangshan, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xei
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College Luzhou Sichuan, China.
authors.forename: Xiong-Fei
authors.initials: XF
authors.lastname: Xu
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College Luzhou Sichuan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College Luzhou Sichuan, China.
authors.forename: Hu-Qiang
authors.initials: HQ
authors.lastname: He
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College Luzhou Sichuan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College Luzhou Sichuan, China.
authors.forename: Ying-Qiang
authors.initials: YQ
authors.lastname: Zheng
authors.affiliation: Deparyment of Vascular Surgery, Affiliated Hospital of Luzhou Medical College Luzhou Sichuan, China.
authors.forename: Yan-Zheng
authors.initials: YZ
authors.lastname: He
keywords: Endothelial progenitor cells; TNFR1; apoptosis; high glucose; oxidative stress reaction