title: Spheroid culture of LuCaP 136 patient-derived xenograft enables versatile preclinical models of prostate cancer.
abstract: LuCaP serially transplantable patient-derived xenografts (PDXs) are valuable preclinical models of locally advanced or metastatic prostate cancer. Using spheroid culture methodology, we recently established cell lines from several LuCaP PDXs. Here, we characterized in depth the features of xenografts derived from LuCaP 136 spheroid cultures and found faithful retention of the phenotype of the original PDX. In vitro culture enabled luciferase transfection into LuCaP 136 spheroids, facilitating in vivo imaging. We showed that LuCaP 136 spheroids formed intratibial, orthotopic, and subcutaneous tumors when re-introduced into mice. Intratibial tumors responded to castration and were highly osteosclerotic. LuCaP 136 is a realistic in vitro-in vivo preclinical model of a subtype of bone metastatic prostate cancer.
authors.affiliation: Department of Urology, Stanford University School of Medicine, Stanford, CA, 94305, USA.|Division of Medicine, Turku University Hospital and University of Turku, Turku, Finland.
authors.forename: Maija P
authors.initials: MP
authors.lastname: Valta
authors.affiliation: Department of Urology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Urology, Stanford University School of Medicine, Stanford, CA, 94305, USA.|Department of Urology and Pediatric Urology, University of Saarland, Homburg/Saar, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Saar
authors.affiliation: Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland.
authors.forename: Johanna
authors.initials: J
authors.lastname: Tuomela
authors.affiliation: Department of Urology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Rosalie
authors.initials: R
authors.lastname: Nolley
authors.affiliation: Department of Urology and Pediatric Urology, University of Saarland, Homburg/Saar, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Linxweiler
authors.affiliation: Cell Imaging Core, Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Jouko
authors.initials: J
authors.lastname: Sandholm
authors.affiliation: Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland.
authors.forename: Jaakko
authors.initials: J
authors.lastname: Lehtimäki
authors.affiliation: Department of Cell Biology and Anatomy, Institute of Biomedicine, University of Turku, Turku, Finland.
authors.forename: Pirkko
authors.initials: P
authors.lastname: Härkönen
authors.affiliation: Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA.
authors.forename: Ilsa
authors.initials: I
authors.lastname: Coleman
authors.affiliation: Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA.|Department of Urology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Nelson
authors.affiliation: Department of Urology, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Eva
authors.initials: E
authors.lastname: Corey
authors.affiliation: Department of Urology, Stanford University School of Medicine, Stanford, CA, 94305, USA. dpeehl@stanford.edu.
authors.forename: Donna M
authors.initials: DM
authors.lastname: Peehl
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology* / Q000556:secondary; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000473:pathology*; D018874:Spheroids, Cellular / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays
keywords: Androgen-dependence; Bone metastasis; Osteosclerosis; Prostate cancer