title: Lupeol enhances inhibitory effect of 5-fluorouracil on human gastric carcinoma cells.
abstract: Lupeol, a dietary triterpene present in many fruits and medicinal plants, has been reported to possess many pharmacological properties including cancer-preventive and anti-cancer effects in vitro and in vivo. Here, we investigated the anti-cancer efficacy and adjuvant chemotherapy action of lupeol in gastric cancer (GC) cells (SGC7901 and BGC823) and explored the underlying mechanisms. Cells were treated with lupeol and/or 5-fluorouracil (5-Fu) and subjected to cell viability, colony formation, apoptosis, western blot, semiquantitative RT-PCR, and xenograft tumorigenicity assay. Our results showed that lupeol and 5-Fu inhibited the proliferation of SGC7901 and BGC823 cells, and combination treatment with lupeol and 5-Fu resulted in a combination index < 1, indicating a synergistic effect. Co-treatment with lupeol and 5-Fu induced apoptosis through up-regulating the expressions of Bax and p53 and down-regulating the expressions of survivin and Bcl-2. Furthermore, co-treatment displayed more efficient inhibition of tumor weight and volume on BGC823 xenograft mouse model than single-agent treatment with 5-Fu or lupeol. Taken together, our findings highlight that lupeol sensitizes GC to 5-Fu treatment, and combination treatment with lupeol and 5-Fu would be a promising therapeutic strategy for human GC treatment.
authors.affiliation: Department of General Surgery, Wujiang No. 1 People's Hospital, Suzhou, 215200, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Geriatric Ward, Wujiang No. 1 People's Hospital, Suzhou, 215200, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Bi
authors.affiliation: Department of General Surgery, Wujiang No. 1 People's Hospital, Suzhou, 215200, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dai
authors.affiliation: Department of General Surgery, Wujiang No. 1 People's Hospital, Suzhou, 215200, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Wujiang No. 1 People's Hospital, Suzhou, 215200, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of General Surgery, Wujiang No. 1 People's Hospital, Suzhou, 215200, China.
authors.forename: Genhai
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of General Surgery, Wujiang No. 1 People's Hospital, Suzhou, 215200, China. wjyygqg@sohu.com.
authors.forename: Quangen
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology / Q000627:therapeutic use; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005472:Fluorouracil / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D008819:Mice, Nude; D053978:Pentacyclic Triterpenes / Q000494:pharmacology / Q000627:therapeutic use; D013274:Stomach Neoplasms / Q000188:drug therapy*; D023041:Xenograft Model Antitumor Assays; D000094042:Lupanes
chemical_list: D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil; D053978:Pentacyclic Triterpenes; C010480:lupeol; D000094042:Lupanes
keywords: 5-Fluorouracil; Apoptosis; Gastric cancer; Lupeol