title: Synthesis, biological evaluation and molecular modeling of pseudo-peptides based statine as inhibitors for human tissue kallikrein 5.
abstract: Human kallikrein 5 (KLK5) is a potential target for the treatment of skin inflammation and cancer. A new series of statine based peptidomimetic compounds were designed and synthesized through simple and efficient reactions. Some KLK5 inhibitors (2a-c compounds) were identified with nanomolar affinity showing Ki values of 0.12-0.13 μM. Our molecular modeling studies suggest that the inhibitors binding at the KLK5 through H-bond interactions with key residues (mainly His108, Gln242, Gly243, Ser245, and Ser260), disrupting the correlated motions mainly among the Ile67-Tyr127, Glu128-Val187, and Gly237-Ser293 subdomains, which seems to be crucial for KLK5 activity. Therefore, we believe that these findings will significantly facilitate our understanding of the conformational dynamics in the course of KLK5 inhibition and, consequently, the development of more potent molecules as alternative for cancer treatment.
authors.affiliation: Laboratório de Química Medicinal, Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mario Viana 523, Santa Rosa, 24241-000, Niterói, RJ, Brazil.
authors.forename: Lucas V B
authors.initials: LVB
authors.lastname: Hoelz
authors.affiliation: Departamento de Química Orgânica, Instituto de Química, Universidade Federal Fluminense, Outeiro de S. João Batista s/n Centro, 24020-141, Niterói, RJ, Brazil.
authors.forename: Bruna C
authors.initials: BC
authors.lastname: Zorzanelli
authors.affiliation: Laboratório de Química Medicinal, Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mario Viana 523, Santa Rosa, 24241-000, Niterói, RJ, Brazil.
authors.forename: Pedro Henrique R de A
authors.initials: PHRA
authors.lastname: Azevedo
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus3, São Bernardo do Campo, SP, Brazil.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Passos
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus3, São Bernardo do Campo, SP, Brazil.
authors.forename: Lucas R
authors.initials: LR
authors.lastname: de Souza
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus3, São Bernardo do Campo, SP, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Zani
authors.affiliation: Departamento de Química Orgânica, Instituto de Química, Universidade Federal Fluminense, Outeiro de S. João Batista s/n Centro, 24020-141, Niterói, RJ, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Pinheiro
authors.affiliation: Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Rua Arcturus3, São Bernardo do Campo, SP, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Puzer
authors.affiliation: Laboratório de Química Medicinal, Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mario Viana 523, Santa Rosa, 24241-000, Niterói, RJ, Brazil.
authors.forename: Luiza R S
authors.initials: LRS
authors.lastname: Dias
authors.affiliation: Laboratório de Química Medicinal, Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mario Viana 523, Santa Rosa, 24241-000, Niterói, RJ, Brazil. Electronic address: estelamuri@yahoo.com.br.
authors.forename: Estela M F
authors.initials: EMF
authors.lastname: Muri
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D007610:Kallikreins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008958:Models, Molecular; D057786:Peptidomimetics / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D004791:Enzyme Inhibitors; D057786:Peptidomimetics; C400147:KLK5 protein, human; D007610:Kallikreins; C013165:statine
keywords: Inhibitors; Kallikreins; Molecular dynamics; Peptidomimetics; Statines