title: Volume Conductivity and Scatter Parameters as an Indicator of Acute Bacterial Infections by the Automated Haematology Analyser.
abstract: BACKGROUND
  Complete blood picture is the first and foremost investigation ordered for evaluation of acute infectious processes which require early and prompt intervention. With the advent of automated Coulter (®) Haematology analysers, the Volume, Conductivity & Scatter parameters of the leucocytes could be analysed for the early detection of the infections.
  
  
  OBJECTIVE
  To evaluate the clinical usefulness of Volume, Conductivity & Scatter parameters of neutrophils & monocytes in predicting the onset of acute bacterial infections.
  
  
  METHODS
  Peripheral blood samples from 94 patients with infections (systemic infections n=36 & localised infections n=58) and 46 control subjects were studied using the Volume, Conductivity & Scatter parameters by the Coulter Haematology analyser.
  
  
  RESULTS
  We observed a significant increase in the mean channel of neutrophil volume & mean channel of monocyte volume from patients with infections (both systemic & localised) as compared with control subjects (Mean Neutrophil Volume: 158.3 ± 13.7 vs 137.2 ± 4.3; p<0.001) & (Mean Monocyte Volume: 177.8± 16 vs 161.7 ± 6.04; p <0.001). However, there was no significant difference in the Mean Neutrophil Volume of patients with systemic infection & localised infections (160.5±17.5 vs 156.8±10.5, p>0.05).
  
  
  CONCLUSIONS
  The Volume, Conductivity & Scatter parameters like Mean Neutrophil Volume & Mean Monocyte Volume are more sensitive parameters and could prove to be a quick diagnostic indicator of acute bacterial infections.
authors.affiliation: Assistant Professor, Department of Pathology, Kasturba Medical College, Manipal University , Mangalore, India .
authors.forename: Pooja K
authors.initials: PK
authors.lastname: Suresh
authors.affiliation: Assistant Professor, Department of Pathology, Kasturba Medical College, Manipal University , Mangalore, India .
authors.forename: Jessica
authors.initials: J
authors.lastname: Minal
authors.affiliation: Asscociate Professor, Department of Pathology, Kasturba Medical College, Manipal University , Mangalore, India .
authors.forename: Purnima S
authors.initials: PS
authors.lastname: Rao
authors.affiliation: Assistant Professor, Department of Community Medicine, Kasturba Medical College, Manipal, Manipal University , Mangalore, India .
authors.forename: Kirthinath
authors.initials: K
authors.lastname: Ballal
authors.affiliation: Assistant Professor, Department of Pathology, Kasturba Medical College, Manipal University , Mangalore, India .
authors.forename: Hanaganahalli B
authors.initials: HB
authors.lastname: Sridevi
authors.affiliation: Senior Resident, Department of Medicine, Kasturba Medical College, Manipal University , Mangalore, India .
authors.forename: Mahesha
authors.initials: M
authors.lastname: Padyana
keywords: Mean monocyte volume; Mean neutrophil volume; Sepsis; VCS parameters