title: Role of ITPA gene polymorphism in ribavirin-induced anemia and thrombocytopenia in Egyptian patients with chronic hepatitis C.
abstract: BACKGROUND
  Ribavirin (RBV)-induced anemia is one of the major causes of dose reduction and discontinuation of therapy for chronic hepatitis C (CHC) patients. We investigated the role of inosine triphosphate pyrophosphatase (ITPA) single nucleotide polymorphism (SNP) (rs1127354) in predicting RBV-induced anemia and thrombocytopenia among Egyptian patients with CHC genotype 4 infection.
  
  
  METHODS
  One hundred and twenty Egyptian patients with CHC genotype 4 who had received standard of care combination therapy were enrolled in this study. Single nucleotide polymorphism at ITPA (rs1127354) was genotyped by real-time detection polymerase chain reaction.
  
  
  RESULTS
  Hb levels between CC and non-CC groups were significantly different at weeks 4, 8, and 12. Hemoglobin decline was significantly higher among CC patient than non-CC patients at week 4 and week 8 of treatment. The RBV dose reduction was higher in CC than non-CC group. Platelet decline was significantly lower in CC patients than non-CC patients at baseline, 4, 12 weeks only.
  
  
  CONCLUSIONS
  Rs1127354 ITPA polymorphism was associated with RBV-induced anemia and thrombocytopenia in Egyptian patients with hepatitis C virus genotype 4 infection.
authors.affiliation: Department of Endemic and Infectious Diseases, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
authors.forename: Nader
authors.initials: N
authors.lastname: Nemr
authors.affiliation: Department of Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. rankishk@yahoo.com.
authors.forename: Rania
authors.initials: R
authors.lastname: Kishk
authors.affiliation: Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Mandour
mesh_terms: D000328:Adult; D000740:Anemia / Q000139:chemically induced*; D000998:Antiviral Agents / Q000008:administration & dosage / Q000009:adverse effects*; D004534:Egypt; D005260:Female; D056726:Genetic Association Studies; D060005:Genotyping Techniques; D019698:Hepatitis C, Chronic / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011755:Pyrophosphatases / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012254:Ribavirin / Q000008:administration & dosage / Q000009:adverse effects*; D013921:Thrombocytopenia / Q000139:chemically induced*; D055815:Young Adult; D000097630:Inosine Triphosphatase
chemical_list: D000998:Antiviral Agents; D011755:Pyrophosphatases; D012254:Ribavirin; C511829:ITPA protein, human; D000097630:Inosine Triphosphatase
keywords: Anemia; Drug induced; Genetic polymorphisms