title: Detection of Arctic and European cluster of canine distemper virus in north and center of Iran.
abstract: Canine distemper virus (CDV) creates a very contagious viral multi-systemic canine distemper (CD) disease that affects most species of Carnivora order. The virus is genetically heterogeneous, particularly in section of the hemagglutinin (H) gene. Sequence analysis of the H gene can be useful to investigate distinction of various lineages related to geographical distribution and CDV molecular epidemiology. Since vaccination program is conducted only in large cities of Iran, CD still remains as one of the major causes of death in dogs in this country. In order to monitor H gene, CDV has been detected in 14 out of 19 sampled dogs through the amplification of nucleoprotein (NP) gene in nested-PCR assay. In the next step 665 bp of H gene was amplified in 9 out of 14 NP-gene positive dogs. Phylogenetic analysis distinguished two distinct CDV genotypes in Iran. JN941238 has been embedded in European cluster and JN941239 has been embedded in Arctic cluster. Nucleic analysis has been shown high difference among both Iranian CDV lineages with CDV vaccine strains.
authors.affiliation: Department of Environmental Sciences, Faculty of fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran;
authors.forename: Somayeh
authors.initials: S
authors.lastname: Namroodi
authors.affiliation: Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;
authors.forename: Amir
authors.initials: A
authors.lastname: Rostami
authors.affiliation: Tasnim Biotechnology Research Center, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran;
authors.forename: Keyvan
authors.initials: K
authors.lastname: Majidzadeh-Ardebili
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;
authors.forename: Arash
authors.initials: A
authors.lastname: Ghalyanchi Langroudi
authors.affiliation: Department of Microbiology, Islamic Azad University of Qom Branch, Qom, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Morovvati
keywords: Canine distemper virus; Hemagglutinin gene; Nucleoprotein gene; Phylogenetic analysis