title: Relationship between manganese superoxide dismutase (MnSODAla-9Val) and glutathione peroxidase (GPx1 Pro 197 Leu) gene polymorphisms and alopecia areata.
abstract: OBJECTIVE
  The role of the oxidative stress in alopecia areata (AA) has been studied by several researchers in a few studies with conflicting results. These results suggested that lipid peroxidation and alterations in the oxidant-antioxidant enzymatic system may play a role in the pathogenesis of AA. Therefore, we aimed to examine the possible associations between the MnSOD Ala-9Val and GPx1 Pro 198 Leu polymorphisms and AA susceptibility and disease progression in Turkish population.
  
  
  METHODS
  The study group consisted of 119 unrelated patients with AA and 104 unrelated healthy controls with no scalp lesions in their personal history or on clinical examination. Genotyping was performed to identify MnSOD Ala-9Val and GPx1 Pro 198 Leu polymorphisms by a method based on PCR amplification and detection of polymorphisms with hybridization probes labeled with fluorescent dyes. Genotype and allele frequencies were compared between patients with AA and healthy control subjects.
  
  
  RESULTS
  There was no significant difference between the MnSOD Ala-9Val SNP genotype distributions and allele frequencies of the AA patients and the control group (P=0.168 and P=0.820, respectively). There was not any association between clinical and demographical features of the study patients with AA and MnSOD Ala-9Val and GPx1 Pro 198 Leu polymorphism genotypes except gender.
  
  
  CONCLUSIONS
  This study is unique since an investigation to reveal the possible associations between the MnSOD Ala-9Val and GPx1 Pro 198 Leu polymorphisms and AA susceptibility and in Turkish population.
authors.affiliation: Department of Dermatology, School of Medicine, Yıldırım Beyazıt University Ankara, Turkey.
authors.forename: Göknur
authors.initials: G
authors.lastname: Kalkan
authors.affiliation: Department of Dermatology, School of Medicine, Gaziosmanpasa University Tokat, Turkey.
authors.forename: Havva Yıldız
authors.initials: HY
authors.lastname: Seçkin
authors.affiliation: Department of Biochemistry, School of Medicine, Gaziosmanpasa University Tokat, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Benli
authors.affiliation: Department of Biochemistry, School of Medicine, Gaziosmanpasa University Tokat, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Akbaş
authors.affiliation: Department of Dermatology, School of Medicine, Gaziosmanpasa University Tokat, Turkey.
authors.forename: Yalçın
authors.initials: Y
authors.lastname: Baş
authors.affiliation: Department of Medical Biology, School of Medicine, Gaziosmanpasa University Tokat, Turkey.
authors.forename: Nevin
authors.initials: N
authors.lastname: Karakus
authors.affiliation: Department of Biochemistry, School of Medicine, Gaziosmanpasa University Tokat, Turkey.
authors.forename: İlknur
authors.initials: İ
authors.lastname: Bütün
authors.affiliation: Department of Biochemistry, School of Medicine, Gaziosmanpasa University Tokat, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Özyurt
keywords: Alopecia areata; GPx1 Pro 198 Leu; MnSOD Ala-9Val; polymorphism