title: Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.
abstract: We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus.
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. nbaenas@cebas.csic.es.|Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Murcia, Spain. nbaenas@cebas.csic.es.
authors.forename: Nieves
authors.initials: N
authors.lastname: Baenas
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. piegholdt@foodsci.uni-kiel.de.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Piegholdt
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. schloesser@foodsci.uni-kiel.de.
authors.forename: Anke
authors.initials: A
authors.lastname: Schloesser
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Murcia, Spain. dmoreno@cebas.csic.es.
authors.forename: Diego A
authors.initials: DA
authors.lastname: Moreno
authors.affiliation: Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo 25, 30100 Murcia, Spain. cgviguera@cebas.csic.es.
authors.forename: Cristina
authors.initials: C
authors.lastname: García-Viguera
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. rimbach@foodsci.uni-kiel.de.
authors.forename: Gerald
authors.initials: G
authors.lastname: Rimbach
authors.affiliation: Institute of Human Nutrition and Food Science, University of Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany. wagner@molecularnutrition.uni-kiel.de.
authors.forename: Anika E
authors.initials: AE
authors.lastname: Wagner
mesh_terms: D000818:Animals; D003545:Cysteine / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D017879:Isothiocyanates / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D023421:Models, Animal; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D042925:Positive Transcriptional Elongation Factor B / Q000378:metabolism; D031224:Raphanus / Q000737:chemistry*; D036226:Seedlings / Q000737:chemistry*
chemical_list: D029721:Drosophila Proteins; D017879:Isothiocyanates; D010936:Plant Extracts; C527201:pgc protein, Drosophila; D042925:Positive Transcriptional Elongation Factor B; D005947:Glucose; D003545:Cysteine; C473643:sulphoraphene
keywords: Brassicaceae; energy metabolism; radish; spargel; sulforaphene