title: Dual-targeting superparamagnetic iron oxide nanoprobes with high and low target density for brain glioma imaging.
abstract: A major limit of superparamagnetic iron oxide nanoparticles (SPIONs) as a magnetic resonance (MR) imaging nanoprobe in clinical applications is that the SPIONs are unable to reach sufficient concentrations at the tumor site by passive targeting to produce an obvious contrast effect for tumor imaging. Single-targeting SPIONs systems have been applied to improve the contrast effect. However, they still suffer from a lack of efficiency and specificity of the SPIONs to tumors. Herein, we developed folic acid (FA) and cyclic Arg-Gly-Asp-D-Tyr-Lys (c(RGDyK)) dual-targeting nanoprobes based on Cy5.5 labeled Fe3O4 nanoparticles (NPs). The synergistic targeting ability of the dual-targeting Fe3O4 NPs and the effect of the dual-target density on targeting specificity were investigated in brain glioma-bearing mice. In vivo T2-weighted MR imaging of brain glioma-bearing mice and ex vivo near-infrared imaging of brains harboring gliomas suggested that the combination of dual-target increased the uptake of NPs by glioma, consequently, enhanced the contrast effect. Moreover, it was revealed that the density of dual-target plays an important role in targeting specificity.
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Anatomy, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, PR China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Ai
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, PR China. Electronic address: yancx65828@163.com.
authors.forename: Changxiang
authors.initials: C
authors.lastname: Yan
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China. Electronic address: lingye@ccmu.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000175:diagnosis*; D016503:Drug Delivery Systems; D005910:Glioma / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D057054:Molecular Imaging; D009374:Neoplasms, Experimental / Q000175:diagnosis
chemical_list: D058185:Magnetite Nanoparticles
keywords: Brain gliomas; FA-c(RGDyK) dual-target; Iron oxide nanoparticles; MR/NIR imaging