title: [Antiproliferative effect of silencing LSD1 gene on Jurkat cell line and its mechanism].
abstract: OBJECTIVE
  To investigate the effect of silencing LSD1 gene by RNA interference on the proliferation, apoptosis on human lymphocytic leukemia Jurkat cell line and its mechanism.
  
  
  METHODS
  The hairpin- like oligonucleotide sequences targeting LSD1 gene was transfected into Jurkat cells by lipofectamine(TM) 2000. The LSD1 mRNA and protein were detected by RQ- PCR and Western blot. Cell growth was determined by MTT. Cell apoptosis was analyzed by flow cytometry. The expression of Bcl-2, Bax, procaspase- 3, and histone H3K4me, H3K4me2, H3K4me3, Act- H3, H3K9me were detected by Western blot.
  
  
  RESULTS
  LSD1 mRNA was markedly suppressed by the shRNA targeting LSD1. LSD1 shRNA suppressed the proliferation and induced cells apoptosis of Jurkat cells. The cell apoptotic rate was (41.34±3.58)%, (3.45±1.54)%, (1.76±0.52)% in LSD1 shRNA, Neg-shRNA and Blank respectively, the difference among them was statistically significant (P<0.05). LSD1 shRNA down- regulated the expressions of Bcl- 2 and procaspase- 3, and up- regulated the expression of Bax. The methylation of H3K4me1, me2 and acetylation of Act- H3 improved without change of the methylation of H3K4me3.
  
  
  CONCLUSIONS
  Deplete of LSD1 gene maybe through modifying the methylation of histone H3K4 to promote the cell apoptosis and inhibit cell growth in Jurkat cell line.
authors.affiliation: Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Han
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Huang
authors.forename: Ruiji
authors.initials: R
authors.lastname: Zheng
mesh_terms: D000107:Acetylation; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D056466:Histone Demethylases / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D006801:Humans; D019169:Jurkat Cells; D008745:Methylation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: C000595707:BCL2 protein, human; D006657:Histones; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D056466:Histone Demethylases; C495195:KDM1A protein, human; C505407:CASP3 protein, human; D053148:Caspase 3