title: Purification, characterization and in vitro anticoagulant activity of polysaccharides from Gentiana scabra Bunge roots.
abstract: Three water-soluble polysaccharide fractions (GSP-1, GSP-2 and GSP-3) were obtained from Gentiana scabra Bunge roots by DEAE-Sepharose CL-6B and Sepharose CL-6B column chromatography. Their chemical characterizations were determined by high performance gel permeation chromatography (HPGPC), high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and Fourier transform infrared (FT-IR) spectrometer. Moreover, their in vitro anticoagulant activities were evaluated by activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) assays. GSP-1 and GSP-2 were composed of rhamnose, arabinose, galactose, glucose and galacturonic acid, while GSP-3 consisted of rhamnose, arabinose, galactose and galacturonic acid with a weight-average molecular weight of 5.8×10(4)Da. In comparison with the control group (saline), GSP, GSP-1, GSP-2 and GSP-3 could prolong APTT and TT, but not PT. Overall, GSP-3 exhibited potent anticoagulant activity and would be expected to be a potential source of anticoagulant.
authors.affiliation: College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China. Electronic address: caiweirong0781@163.com.
authors.forename: Weirong
authors.initials: W
authors.lastname: Cai
authors.affiliation: College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Xu
authors.affiliation: College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Xie
authors.affiliation: College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Sun
authors.affiliation: College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China.
authors.forename: Qinbao
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D030012:Gentiana / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011522:Protons
chemical_list: D000925:Anticoagulants; D009005:Monosaccharides; D011134:Polysaccharides; D011522:Protons
keywords: Anticoagulant activity; Characterization; Gentiana scabra Bunge; Polysaccharides; Purification