title: Rapid evaluation of artesunate quality with a specific monoclonal antibody-based lateral flow dipstick.
abstract: Artesunate is a frontline antimalarial drug for treating Plasmodium falciparum malaria. To produce specific antibodies to artesunate, the carboxyl group of artesunate was directly conjugated to carrier protein as the immunogen. A specific monoclonal antibody (mAb) 3D82G6 against artesunate was obtained by high-throughput screening of positive hybridoma clones. This monoclonal antibody had 4.0, 0.5, and 0.9 % cross reactivities with artemisinin, dihydroartemisinin, and artemether, respectively. A dipstick immunoassay was developed, and the indicator range for artesunate was 1000-2000 ng mL(-1). No interference was observed with artemisinin, dihydroartemisinin, artemether, and other commonly used antimalarial drugs for up to 20,000 ng mL(-1). The dipsticks were used for determination of artesunate contents in commercial drugs, and the results were agreeable with those determined by high-performance liquid chromatography. This dipstick, with its specificity and sensitivity for artesunate and simplicity to use, makes it a potential point-of-care device for rapid quality evaluation of artesunate-containing antimalarial drugs. Graphical Abstract Specific monoclonal antibody-based lateral flow dipstick for artesunate.
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Suqin
authors.initials: S
authors.lastname: Guo
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Lishan
authors.initials: L
authors.lastname: He
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
authors.forename: Guiyu
authors.initials: G
authors.lastname: Tan
authors.affiliation: Myanmar Medical Association, No. 249, Thein Phyu Road, Mingalartaungnyunt Township, Yangon, Myanmar.
authors.forename: Myo
authors.initials: M
authors.lastname: Min
authors.affiliation: Department of Medical Research, Ministry of Health, No. 5, Ziwaka Road, Dagon Township, Yangon, Myanmar.
authors.forename: Myat Phone
authors.initials: MP
authors.lastname: Kyaw
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China. wbaomin@263.net.
authors.forename: Baomin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Entomology, Pennsylvania State University, University Park, PA, 16802, USA. luc2@psu.edu.
authors.forename: Liwang
authors.initials: L
authors.lastname: Cui
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000962:Antimalarials / Q000032:analysis* / Q000276:immunology; D037621:Artemisinins / Q000032:analysis* / Q000276:immunology; D000077332:Artesunate; D017979:Gold Colloid / Q000737:chemistry; D006801:Humans; D006825:Hybridomas; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D016778:Malaria, Falciparum / Q000188:drug therapy; D051379:Mice; D019095:Point-of-Care Systems; D011934:Reagent Strips / Q000032:analysis
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D000962:Antimalarials; D037621:Artemisinins; D017979:Gold Colloid; D011934:Reagent Strips; D000077332:Artesunate
keywords: Antibody; Artesunate; Dipstick; Malaria