title: Bone marrow stromal cells induced activation of nuclear factor κB signaling protects non-Hodgkin's B lymphoma cells from apoptosis.
abstract: The microenvironment encompassing a variety of non-malignant cells in close proximity with malignant tumor cells has been well known to significantly affect the behavior of tumor cells. In this study, we therefore studied the mechanism of bone marrow stromal cells in protection of lymphoma cells from spontaneous apoptosis. We demonstrated that adhesion of the freshly isolated lymphoma B cells to bone marrow stromal cells or freshly isolated lymphoma stromal cells inhibited B cell spontaneous apoptosis in culture. This inhibition of apoptosis correlated with decreased cleavage of caspase-3/8 and increased activation of canonical and non-canonical NF-κB signaling pathway. In addition to BAFF signaling which has been reported as a functional determinant for B lymphoma cell survival in the bone marrow environment, we demonstrated RANKL from BMSCs works synergistically with BAFF to activate NF-κB signaling pathway and thus protects lymphoma B cells from spontaneous apoptosis.
authors.affiliation: Department of General Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Su
authors.affiliation: Department of Radiology of Chinese PLA General Hospital, Beijing, China.
authors.forename: Jiakai
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Meng
authors.affiliation: Department of Cardiology, Peking University Ninth School of Clinical Medicine, Beijing Shijitan Hospital, Beijing, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of General Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of General Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of General Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China. chr_doctor@126.com.
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, China. leipan2010@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Special Medical Treatment, Beijing Shijitan Hospital, Capital Medical University, 10 Tieyi Road, Yangfang District, Beijing, China. xinyingxue2010@163.com.
authors.forename: Xinying
authors.initials: X
authors.lastname: Xue
mesh_terms: D017209:Apoptosis / Q000502:physiology; D053264:B-Cell Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D016328:NF-kappa B / Q000502:physiology*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D061251:Primary Cell Culture; D053245:RANK Ligand / Q000037:antagonists & inhibitors / Q000502:physiology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured; D059016:Tumor Microenvironment
chemical_list: D053264:B-Cell Activating Factor; D016328:NF-kappa B; D009363:Neoplasm Proteins; D053245:RANK Ligand; D034741:RNA, Small Interfering; C505688:TNFSF11 protein, human; C505719:TNFSF13B protein, human
keywords: Apoptosis; Bone marrow stromal cells; Non-Hodgkin’s B lymphoma cells; Nuclear factor κB signaling