title: Decreased expression of hsa_circ_001988 in colorectal cancer and its clinical significances.
abstract: Circular RNA (circRNA) is a type of RNAs which, unlike the better known linear RNA, forms a covalently closed continuous loop. They have emerged recently as a new player in governing fundamental biological processes. However it remains elusive about the correlation of hsa_circ_001988 abundance with colorectal cancer. To investigate the circular RNA expression in colorectal cancer, the targeted hsa_circ_001988 was selected from next generation sequence data base generated in house and then designed divergent primers to amplify hsa_circ_001988 and sequenced it for validation. The expression of hsa_circ_001988 in 31 matched colorectal cancer tissue and normal colon mucosa was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). We used ΔCt method and investigated the differences between tumor tissues and normal colon mucosa by paired t-test. One-way analysis of variance was conducted to analyze the relationship between hsa_circ_001988 expression level and clinic pathological factors of patients. Receiver operating characteristic (ROC) curve was built by SPSS to evaluate the diagnostic values. The expression of hsa_circ_001988 was significantly correlated with differentiation (P<0.05) and perineural invasion (P<0.05). The area under ROC curve of hsa_circ_001988 was 0.788 (P<0.05). Those results indicate that hsa_circ_001988 may become a novel potential biomarker in the diagnosis of colorectal cancer and a potential novel target for the treatment of colorectal cancer.
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Xuning
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Jiajin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology and Hepatology, Chinese PLA General Hospital Beijing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pan
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Baoqing
authors.initials: B
authors.lastname: Jia
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences Beijing, China.
authors.forename: Shiyou
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology, Chinese PLA General Hospital Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D012313:RNA / Q000235:genetics*; D000079962:RNA, Circular; D012334:RNA, Neoplasm / Q000235:genetics*; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D000079962:RNA, Circular; D012334:RNA, Neoplasm; D012313:RNA
keywords: Circular RNA; biomarker; colorectal cancer; differentiation; hsa_circ_001988