title: miR-130b targets NKD2 and regulates the Wnt signaling to promote proliferation and inhibit apoptosis in osteosarcoma cells.
abstract: miR-130b was significantly up-regulated in osteosarcoma (OS) cells. Naked cuticle homolog 2 (NKD2) inhibited tumor growth and metastasis in OS by suppressing Wnt signaling. We used three miRNA target analysis tools to identify potential targets of miR-130b, and found that NKD2 is a potential target of miR-130b. Based on these findings, we hypothesize that miR-130b might target NKD2 and regulate the Wnt signaling to promote OS growth. We detected the expression of miR-130b and NKD2 mRNA and protein by quantitative Real-Time PCR (qRT-PCR) and western blot assays, respectively, and found up-regulation of miR-130b and down-regulation of NKD2 mRNA and protein exist in OS cell lines. MTT and flow cytometry assays showed that miR-130b inhibitors inhibit proliferation and promote apoptosis in OS cells. Furthermore, we showed that NKD2 is a direct target of miR-130b, and miR-130b regulated proliferation and apoptosis of OS cells by targeting NKD2. We further investigated whether miR-130b and NKD2 regulate OS cell proliferation and apoptosis by inhibiting Wnt signaling, and the results confirmed our speculation that miR-130b targets NKD2 and regulates the Wnt signaling to promote proliferation and inhibit apoptosis of OS cells. These findings will offer new clues for OS development and progression, and novel potential therapeutic targets for OS.
authors.affiliation: Department of Human Anatomy and Histoembryology, College of Basic Medical Sciences, Jilin University, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Human Anatomy and Histoembryology, College of Basic Medical Sciences, Jilin University, China. Electronic address: liyoujunn@126.com.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Central Hospital Affiliated to Shenyang Medical College, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Central Hospital Affiliated to Shenyang Medical College, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yang
authors.affiliation: Central Hospital Affiliated to Shenyang Medical College, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Central Hospital Affiliated to Shenyang Medical College, China.
authors.forename: Xiandong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017209:Apoptosis / Q000235:genetics; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; C449092:NKD2 protein, human
keywords: Apoptosis; Naked cuticle homolog 2; Osteosarcoma; Proliferation; miR-130b