title: Pro-apoptotic and anti-angiogenic properties of the α /β-thujone fraction from Thuja occidentalis on glioblastoma cells.
abstract: The most aggressive type of brain tumor is glioblastoma multiforme, which to date remains incurable. Thuja occidentalis is used in homeopathy for the treatment of cancer, however, its mechanism of action remains unknown. We set out to study the effects of thujone fractions of Thuja on glioblastoma using in vitro and in vivo models. We found that the α/ β-thujone fraction decrease the cell viability and exhibit a potent anti-proliferative, pro-apoptotic and anti-angiogenic effects in vitro. In vivo assays showed that α /β-thujone promotes the regression of neoplasia and inhibits the angiogenic markers VEGF, Ang-4 and CD31 into the tumor.
authors.affiliation: Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, P.O. Box 567, Valdivia, Chile.
authors.forename: Angelo
authors.initials: A
authors.lastname: Torres
authors.affiliation: Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, P.O. Box 567, Valdivia, Chile.
authors.forename: Yosselyn
authors.initials: Y
authors.lastname: Vargas
authors.affiliation: Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, P.O. Box 567, Valdivia, Chile.
authors.forename: Daniel
authors.initials: D
authors.lastname: Uribe
authors.affiliation: Departamento de Patología del Hospital Base de Valdivia (HBV), Valdivia, Chile.
authors.forename: Cristian
authors.initials: C
authors.lastname: Carrasco
authors.affiliation: Departamento de Patología del Hospital Base de Valdivia (HBV), Valdivia, Chile.
authors.forename: Cristian
authors.initials: C
authors.lastname: Torres
authors.affiliation: Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, P.O. Box 567, Valdivia, Chile.
authors.forename: René
authors.initials: R
authors.lastname: Rocha
authors.affiliation: Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, P.O. Box 567, Valdivia, Chile.
authors.forename: Carlos
authors.initials: C
authors.lastname: Oyarzún
authors.affiliation: Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, P.O. Box 567, Valdivia, Chile.
authors.forename: Rody
authors.initials: R
authors.lastname: San Martín
authors.affiliation: Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja s/n, P.O. Box 567, Valdivia, Chile. claudiaquezada@uach.cl.
authors.forename: Claudia
authors.initials: C
authors.lastname: Quezada
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000081008:Bicyclic Monoterpenes; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005909:Glioblastoma / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008297:Male; D039821:Monoterpenes / Q000494:pharmacology*; D009368:Neoplasm Transplantation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D029783:Thuja
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D010936:Plant Extracts; C005790:beta-thujone
keywords: Angiogenesis; Angiopoietin-4; Apoptosis; Glioblastoma multiforme; Thuja occidentalis; α / β-Thujone fraction