title: [Effects of stromal cell-derived factor-1 on proliferation, migration, and odontoblastic differentiation of human dental pulp stem cells].
abstract: OBJECTIVE
  To compare the effects of stromal cell-derived factor-1 (SDF-1) and granulocyte colony-stimulating factor (G-CSF) on proliferation, migration, and odontoblastic differentiation of human dental pulp stem cell (DPSC) in vitro.
  
  
  METHODS
  DPSCs were cultured in vitro and treated with either 100 μg/L SDF-1 or 100 μg/L G-CSF. Cell counting kit-8 (CCK-8) and colony-forming unit (CFU) were used to detect the effect of SDF-1 and G -CSF on the proliferation ability of DPSC. Cell migration of DPSC was determined by wound healing assay and Transwell migration assay. The effects of SDF-1 and G-CSF on odontoblastic differentiation of DPSC were evaluated by alkaline phosphatase (ALP) staining, ALP activity and alizarin red S staining. The expression of odontoblastic-related genes such as dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP) were quantified by real-time RT-PCR.
  
  
  RESULTS
  SDF-1 and G-CSF promoted the proliferation of DPSC slightly, but the difference was not statistically significant. Wound healing assay showed that SDF-1 and G-CSF promoted cell migration of DPSC significantly (P<0.01), but there was no significant difference between the two factors. In Transwell migration assay, the number of migrated cells of the control group was 5.0 ± 1.4 per sight, while the SDF-1 group was 24.3 ± 6.8 per sight and the G-CSF group was 11.8 ± 3.3 per sight, suggesting that cell migration of DPSC was improved significantly after being treated with SDF-1 or G-CSF, and SDF-1 was more effective than G-CSF (P<0.05). Significantly greater odontoblastic differentiation potential was found in SDF-1 group and G-CSF group based on the ALP staining. Higher ALP activity, more mineralization nodule formation and higher expressions of DMP-1 and DSPP were also found after SDF-1 or G-CSF treatment.
  
  
  CONCLUSIONS
  SDF-1 had no significant effect on the proliferation of DPSC, but could significantly promote cell migration and odontoblastic differentiation of DPSC. Its effect on DPSC was better than G-CSF.
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Yu-ming
authors.initials: YM
authors.lastname: Zhao
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Wang
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ling
authors.affiliation: Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Ge
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D009804:Odontoblasts / Q000166:cytology* / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D000099311:Dentin Sialophosphoprotein
chemical_list: D054377:Chemokine CXCL12; D016326:Extracellular Matrix Proteins; D016179:Granulocyte Colony-Stimulating Factor; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C515255:CXCL12 protein, human; C513309:DMP1 protein, human