title: Changes of soil prokaryotic communities after clear-cutting in a karst forest: evidences for cutting-based disturbance promoting deterministic processes.
abstract: To understand the temporal responses of soil prokaryotic communities to clear-cutting disturbance, we examined the changes in soil bacterial and archaeal community composition, structure and diversity along a chronosequence of forest successional restoration using high-throughput 16S rRNA gene sequencing. Our results demonstrated that clear-cutting significantly altered soil bacterial community structure, while no significant shifts of soil archaeal communities were observed. The hypothesis that soil bacterial communities would become similar to those of surrounding intact primary forest with natural regeneration was supported by the shifts in the bacterial community composition and structure. Bacterial community diversity patterns induced by clear-cutting were consistent with the intermediate disturbance hypothesis. Dynamics of bacterial communities was mostly driven by soil properties, which collectively explained more than 70% of the variation in bacterial community composition. Community assembly data revealed that clear-cutting promoted the importance of the deterministic processes in shaping bacterial communities, coinciding with the resultant low resource environments. But assembly processes in the secondary forest returned a similar level compared to the intact primary forest. These findings suggest that bacterial community dynamics may be predictable during the natural recovery process.
authors.affiliation: Key Laboratory of Forest Ecology and Environment, China's State Forestry Administration, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, No. 2 Dongxiaofu, Haidian District, Beijing 100091, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Forest Ecology and Environment, China's State Forestry Administration, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, No. 2 Dongxiaofu, Haidian District, Beijing 100091, China liusr@caf.ac.cn.
authors.forename: Shirong
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, CAS; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan, 610041, PR China.
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Li
authors.affiliation: Division of Forestry and Natural Resources, West Virginia University, P.O. Box 6215, Morgantown, WV 26506-6125, USA.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Horticulture and Landscape Architecture, Hainan University, No.58, Renmin Road, Meilan District, Haikou, Hainan Province, 570228, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Key Laboratory of Forest Ecology and Environment, China's State Forestry Administration, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, No. 2 Dongxiaofu, Haidian District, Beijing 100091, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Earth Sciences, Indiana University-Purdue University, Indianapolis (IUPUI), Indianapolis, Indiana 46202, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Tian
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, CAS; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Sichuan, 610041, PR China.
authors.forename: Minjie
authors.initials: M
authors.lastname: Yao
authors.affiliation: West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: An
authors.affiliation: Key Laboratory of Forest Ecology and Environment, China's State Forestry Administration, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, No. 2 Dongxiaofu, Haidian District, Beijing 100091, China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Huang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D065928:Forests; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014197:Trees / Q000254:growth & development / Q000382:microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: archaeal community structure; bacterial community structure; clear-cutting; community assembly; deterministic processes; diversity