title: Alternariol 9-methyl ether from the endophytic fungus Alternaria sp. Samif01 and its bioactivities.
abstract: One bioactive compound, identified as alternariol 9-methyl ether, was isolated from the crude extract of the endophytic fungus Alternaria sp. Samif01 residing in the roots of Salvia miltiorrhiza Bunge. Alternariol 9-methyl ether was active against bacteria with minimum inhibitory concentration values ranging from 25 to 75μg/mL and median inhibitory concentration (IC50) values ranging from 16.00 to 38.27μg/mL. The IC50 value of alternariol 9-methyl ether against spore germination of Magnaporthe oryzae was 87.18μg/mL. Alternariol 9-methyl ether also showed antinematodal activity against Bursaphelenchus xylophilus and Caenorhabditis elegans with IC50 values of 98.17μg/mL and 74.62μg/mL, respectively. This work is the first report on alternariol 9-methyl ether and its biological activities from the endophytic fungus Alternaria sp. Samif01 derived from S. miltiorrhiza Bunge. The results indicate the potential of Alternaria sp. Samif01 as a source of alternariol 9-methyl ether and also support that alternariol 9-methyl ether is a natural compound with high potential bioactivity against microorganisms.
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Lou
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Ruiting
authors.initials: R
authors.lastname: Yu
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Wang
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Mao
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Linyun
authors.initials: L
authors.lastname: Fu
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: MOA Key Laboratory of Plant Pathology, Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: lgzhou@cau.edu.cn.
authors.forename: Ligang
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000528:Alternaria / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D060026:Endophytes / Q000737:chemistry* / Q000302:isolation & purification; D020128:Inhibitory Concentration 50; D007783:Lactones / Q000302:isolation & purification* / Q000378:metabolism; D020082:Magnaporthe / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009348:Nematoda / Q000187:drug effects; D018517:Plant Roots / Q000382:microbiology; D027544:Salvia / Q000382:microbiology
chemical_list: D000890:Anti-Infective Agents; D007783:Lactones; C018206:alternariol monomethyl ether
keywords: Alternaria sp., Alternariol 9-methyl ether; Bioactivity; Endophytic fungus; Salvia miltiorrhiza Bunge