title: Mechanical characterization of the P56 mouse brain under large-deformation dynamic indentation.
abstract: The brain is a complex organ made up of many different functional and structural regions consisting of different types of cells such as neurons and glia, as well as complex anatomical geometries. It is hypothesized that the different regions of the brain exhibit significantly different mechanical properties, which may be attributed to the diversity of cells and anisotropy of neuronal fibers within individual brain regions. The regional dynamic mechanical properties of P56 mouse brain tissue in vitro and in situ at velocities of 0.71-4.28 mm/s, up to a deformation of 70 μm are presented and discussed in the context of traumatic brain injury. The experimental data obtained from micro-indentation measurements were fit to three hyperelastic material models using the inverse Finite Element method. The cerebral cortex elicited a stiffer response than the cerebellum, thalamus, and medulla oblongata regions for all velocities. The thalamus was found to be the least sensitive to changes in velocity, and the medulla oblongata was most compliant. The results show that different regions of the mouse brain possess significantly different mechanical properties, and a significant difference also exists between the in vitro and in situ brain.
authors.affiliation: School of Mechanical &Materials Engineering, University College Dublin, Dublin, Ireland.
authors.forename: David B
authors.initials: DB
authors.lastname: MacManus
authors.affiliation: School of Mechanical &Materials Engineering, University College Dublin, Dublin, Ireland.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Pierrat
authors.affiliation: Department of Mechanical &Manufacturing Engineering, Dublin City University, Dublin, Ireland.
authors.forename: Jeremiah G
authors.initials: JG
authors.lastname: Murphy
authors.affiliation: School of Mechanical &Materials Engineering, University College Dublin, Dublin, Ireland.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Gilchrist
mesh_terms: D000818:Animals; D016880:Anisotropy; D000070642:Brain Injuries, Traumatic / Q000473:pathology*; D001931:Brain Mapping; D002540:Cerebral Cortex / Q000033:anatomy & histology*; D003198:Computer Simulation; D004548:Elasticity; D020342:Finite Element Analysis; D051379:Mice; D013314:Stress, Mechanical