title: Resveratrol Protects against Titanium Particle-Induced Aseptic Loosening Through Reduction of Oxidative Stress and Inactivation of NF-κB.
abstract: Aseptic implant loosening is closely associated with chronic inflammation induced by implant wear debris, and reactive oxygen species (ROS) play an important role in this process. Resveratrol, a plant compound, has been reported to act as an antioxidant in many inflammatory conditions; however, its protective effect and mechanism against wear particle-induced oxidative stress remain unknown. In this study, we evaluated resveratrol's protective effects against wear particle-induced oxidative stress in RAW 264.7 macrophages. At non-toxic concentrations, resveratrol showed dose-dependent inhibition of nitric oxide (NO) production, ROS generation, and lipid peroxidation. It also downregulated the gene expression of oxidative enzymes, including inducible nitric oxide synthase (iNOS) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX)-1 and NOX-2, and promoted the gene expression and activities of antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx). This protective effect against wear particle-induced oxidative stress was accompanied by a reduction of gene expression and release of tumor necrosis factor-α (TNF-α), and decreased gene expression and phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). These findings demonstrate that resveratrol can inhibit wear particle-induced oxidative stress in macrophages, and may exert its antioxidant effect and protect against aseptic implant loosening.
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
authors.forename: Guotian
authors.initials: G
authors.lastname: Luo
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Li
authors.affiliation: Allergy Center, Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
authors.forename: Haixing
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
authors.forename: Ziji
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
authors.forename: Weishen
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
authors.forename: Yangchun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China.
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
authors.forename: Chaohong
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, NO. 107, Yan Jiang Xi Road, Guangzhou, 510120, People's Republic of China. yingguo1996@gmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Joint Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan Rd. 2, Guangzhou, 510080, People's Republic of China. shengpuyi@hotmail.com.
authors.forename: Puyi
authors.initials: P
authors.lastname: Sheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008264:Macrophages / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis; D000074624:NADPH Oxidase 1; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000096:biosynthesis; D016328:NF-kappa B / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D010014:Osteolysis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D019736:Prostheses and Implants / Q000009:adverse effects*; D000067996:RAW 264.7 Cells; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism; D014025:Titanium / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D014025:Titanium; D002374:Catalase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D013482:Superoxide Dismutase; D009247:NADH, NADPH Oxidoreductases; C488842:Cybb protein, mouse; D000074624:NADPH Oxidase 1; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D005980:Glutathione Reductase; D000077185:Resveratrol
keywords: aseptic loosening; chronic inflammation; oxidative stress; reactive oxygen species (ROS); resveratrol