title: Effects of Tetrahydrocurcumin on Tumor Growth and Cellular Signaling in Cervical Cancer Xenografts in Nude Mice.
abstract: Tetrahydrocurcumin (THC) is a stable metabolite of curcumin (CUR) in physiological systems. The mechanism underlying the anticancer effect of THC is not completely understood. In the present study, we investigated the effects of THC on tumor growth and cellular signaling in cervical cancer xenografts in nude mice. Cervical cancer cells (CaSki) were subcutaneously injected in nude mice to establish tumors. One month after the injection, mice were orally administered vehicle or 100, 300, and 500 mg/kg of THC daily for 30 consecutive days. Relative tumor volume (RTV) was measured every 3-4 days. COX-2, EGFR, p-ERK1&2, p-AKT, and Ki-67 expressions were measured by immunohistochemistry whereas cell apoptosis was detected by TUNELS method. THC treatments at the doses of 100, 300, and 500 mg/kg statistically retarded the RTV by 70.40%, 76.41%, and 77.93%, respectively. The CaSki + vehicle group also showed significantly increased COX-2, EGFR, p-ERK1&2, and p-AKT; however they were attenuated by all treatments with THC. Ki-67 overexpression and a decreasing of cell apoptosis were found in CaSki + vehicle group, but these findings were reversed after the THC treatments.
authors.affiliation: Division of Physiology, Preclinical Science, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani 12120, Thailand.
authors.forename: Bhornprom
authors.initials: B
authors.lastname: Yoysungnoen
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Parvapan
authors.initials: P
authors.lastname: Bhattarakosol
authors.affiliation: Division of Physical Science, Faculty of Science and Technology, Huachiew Chalermprakiet University, Samut Prakan 10540, Thailand.
authors.forename: Chatchawan
authors.initials: C
authors.lastname: Changtam
authors.affiliation: Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Suthiluk
authors.initials: S
authors.lastname: Patumraj
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D003474:Curcumin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D066246:ErbB Receptors / Q000096:biosynthesis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D020935:MAP Kinase Signaling System / Q000187:drug effects; D051379:Mice; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D019394:Ki-67 Antigen; C096277:tetrahydrocurcumin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C512478:EGFR protein, human; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt; D003474:Curcumin