title: Complete genome sequence of mumps viruses isolated from patients with parotitis, pancreatitis and encephalitis in India.
abstract: Limited information is available regarding epidemiology of mumps in India. Mumps vaccine is not included in the Universal Immunization Program of India. The complete genome sequences of Indian mumps virus (MuV) isolates are not available, hence this study was performed. Five isolates from bilateral parotitis and pancreatitis patients from Maharashtra, a MuV isolate from unilateral parotitis patient from Tamil Nadu, and a MuV isolate from encephalitis patient from Uttar Pradesh were genotyped by the standard protocol of the World Health Organization and subsequently complete genomes were sequenced. Indian MuV genomes were compared with published MuV genomes, including reference genotypes and eight vaccine strains for the genetic differences. The SH gene analysis revealed that five MuV isolates belonged to genotype C and two belonged to genotype G strains. The percent nucleotide divergence (PND) was 1.1% amongst five MuV genotype C strains and 2.2% amongst two MuV genotype G strains. A comparison with widely used mumps Jeryl Lynn vaccine strain revealed that Indian mumps isolates had 54, 54, 53, 49, 49, 38, and 49 amino acid substitutions in Chennai-2012, Kushinagar-2013, Pune-2008, Osmanabad-2012a, Osmanabad-2012b, Pune-1986 and Pune-2012, respectively. This study reports the complete genome sequences of Indian MuV strains obtained in years 1986, 2008, 2012 and 2013 that may be useful for further studies in India and globally.
authors.affiliation: National Institute of Virology, Indian Council of Medical Research, 20-A Dr Ambedkar Road, Pune 411001, India. Electronic address: vaidyasr@icmr.org.in.
authors.forename: Sunil R
authors.initials: SR
authors.lastname: Vaidya
authors.affiliation: National Institute of Virology, Indian Council of Medical Research, 20-A Dr Ambedkar Road, Pune 411001, India.
authors.forename: Deepika T
authors.initials: DT
authors.lastname: Chowdhury
authors.affiliation: National Institute of Virology, Indian Council of Medical Research, 20-A Dr Ambedkar Road, Pune 411001, India.
authors.forename: Santoshkumar M
authors.initials: SM
authors.lastname: Jadhav
authors.affiliation: Department of Microbiology, Yogeshwari Mahavidyalaya Ambajogai affiliated to Dr Babasaheb Ambedkar Marathwada University, Aurangabad 431 004, India.
authors.forename: Venkat S
authors.initials: VS
authors.lastname: Hamde
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D018792:Encephalitis, Viral / Q000517:prevention & control / Q000821:virology*; D005260:Female; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007223:Infant; D008297:Male; D008875:Middle Aged; D009107:Mumps / Q000517:prevention & control / Q000821:virology*; D009108:Mumps Vaccine / Q000276:immunology; D009109:Mumps virus / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D010195:Pancreatitis / Q000517:prevention & control / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D006389:Hemagglutinins, Viral; D007075:Immunoglobulin M; D009108:Mumps Vaccine
keywords: Complete genome; Encephalitis; Mumps virus; Pancreatitis; Parotitis; Phylogenetic analysis