title: [Feasibility Study for Storage and Re-Utilization of Circulating Tumor Cells (CTCs)].
abstract: BACKGROUND
  In this era of precision medicine, monitoring patients requires not only real time but also longitudinal sequence of samples at various time points. Based on this background, we focused on conditioned circumstances on fixation and storage for re-utilization of CTCs.
  
  
  METHODS
  Instead of actual CTCs, Cell line (H1975) derived from lung cancer was used because of their scarceness of CTCs.
  
  
  METHODS
  These cells were put on a slide by using an auto-smear device. The slides were evaluated under various centrifuge forces, fixations for the following storages.
  
  
  CONCLUSIONS
  The study indicated that 800 rpm for 1 min centrifuge and fixation by 95% ETOH was excellent. Further at least 5 cells per 1 mL cell solution were required for the following procedures including Fluorescence in situ hybridization (FISH) analysis. This study provides insights of new platform for evaluation of CTCs not only real time but also longitudinal sequence at various time points.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kakishima
authors.forename: Susumu
authors.initials: S
authors.lastname: Wakai
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shibuki
authors.forename: Naoshi
authors.initials: N
authors.lastname: Sasaki
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Koji
authors.initials: K
authors.lastname: Tsuta
authors.forename: Koh
authors.initials: K
authors.lastname: Furuta
mesh_terms: D045744:Cell Line, Tumor; D003584:Cytological Techniques / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000473:pathology; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D013048:Specimen Handling / Q000379:methods*