title: Roles of microRNA-124a and microRNA-30d in breast cancer patients with type 2 diabetes mellitus.
abstract: The aim of the study is to explore roles of microRNA (miR)-124a and miR-30d in breast cancer (BC) patients with type 2 diabetes mellitus (T2DM). A total of 144 cases of confirmed diagnosed BC with T2DM, T2DM, BC, or healthy people were enrolled. Among them, BC patients with T2DM were regarded as the experiment group (n = 36), patients with T2DM as the Dm group (n = 36), patients with BC as the Bc group (n = 36), and healthy subjects as the healthy group (n = 36). The fasting insulin resistance index, glycosylated hemoglobin, and estradiol were measured. MiR-124a and miR-30d expressions were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The insulin resistance index was significantly higher in the experiment group compared to the other three groups (all P < 0.05). The glycated hemoglobin was in a normal range in the Bc group and healthy group, but was higher in the experiment group and the Bc group compared to that in the healthy group (both P < 0.05). The serum estradiol level was obviously higher in the Bc group compared with that in the Dm group and the experiment group (both P < 0.05). The expressions of miR-124a and miR-30d were positively correlated with insulin resistance index, BMI and glycosylated hemoglobin (miR-124a r = 0.659, r = 0.785, and r = 0.862; miR-30d r = 0.742, r = 0.805, r = 0.765; all P < 0.001). Insulin resistance index was an independent factor for expressions of miR124-a and miR-30d. MiR-124a and miR-30d were correlated with insulin resistance and development of BC with T2DM. Although the mechanism is not clear, miR-124a and miR-30d potentially may be used as therapeutic targets and prognostic markers for BC patients with T2DM.
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Ling-Ji
authors.initials: LJ
authors.lastname: Guo
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Ling-Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Hao
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Wu-Guo
authors.initials: WG
authors.lastname: Tian
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China.
authors.forename: Xian-E
authors.initials: XE
authors.lastname: Cao
authors.affiliation: Department of Breast, Thyroid, and Vascular Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Changjiangzhi Road, No 10, Yuzhong Area, Chongqing, 400042, People's Republic of China. LDL_dongDD@163.com.
authors.forename: Dong-Lin
authors.initials: DL
authors.lastname: Luo
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000097:blood / Q000150:complications* / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications* / Q000235:genetics*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D006442:Glycated Hemoglobin; D007328:Insulin; D035683:MicroRNAs; C509207:MIRN124 microRNA, human; C546086:MIRN30b microRNA, human
keywords: Breast cancer; Estradiol; Glycosylated hemoglobin; Insulin resistance index; MiR-124a; MiR-30d; Type 2 diabetes mellitus