title: IL-1β (interleukin-1β) stimulates the production and release of multiple cytokines and chemokines by human preadipocytes.
abstract: The effect of IL-1β on cytokine and chemokine production by human preadipocytes has been examined. Preadipocytes were incubated with IL-1β, and cytokine and chemokine release was measured at 24 h by protein arrays, while the expression of cytokine/chemokine genes was assessed by qPCR at 4 and 24 h. IL-1β stimulated the secretion of multiple cytokines/chemokines, including IL-6, IL-8, IL-10, IL-13, MCP-4, TNFα and IP-10. IL-10 was not released by un-stimulated preadipocytes, while IL-6 exhibited the greatest response to IL-1β (453-fold increase). IL-16 and IL-12p40 did not respond to IL-1β. qPCR demonstrated that IL-1β markedly stimulated CCL3, CSF3 and CXCL10 expression at 4 h (>900-fold mRNA increase). A time-course indicated that while CCL13 (encoding MCP-4) exhibited minimal basal expression in preadipocytes, expression increased progressively following differentiation. Human preadipocytes are highly sensitive to IL-1β, the cytokine stimulating a major inflammatory response in these cells similar to that in mature adipocytes.
authors.affiliation: a Zoology Department, College of Science, King Saud University , Riyadh , Saudi Arabia .
authors.forename: Suliman Y
authors.initials: SY
authors.lastname: Alomar
authors.affiliation: b Clore Laboratory, University of Buckingham , Buckingham , UK , and.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gentili
authors.affiliation: b Clore Laboratory, University of Buckingham , Buckingham , UK , and.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Zaibi
authors.affiliation: b Clore Laboratory, University of Buckingham , Buckingham , UK , and.
authors.forename: Malgorzata A
authors.initials: MA
authors.lastname: Kępczyńska
authors.affiliation: a Zoology Department, College of Science, King Saud University , Riyadh , Saudi Arabia .|b Clore Laboratory, University of Buckingham , Buckingham , UK , and.|c Obesity Biology Unit, University of Liverpool , Duncan Building , Liverpool , UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Trayhurn
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D040081:Protein Array Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018925:Chemokines; D016207:Cytokines; D053583:Interleukin-1beta
keywords: Adipocytes; adipose tissue; gene expression; inflammation; protein arrays