title: Rationalizing the use of functionalized poly-lactic-co-glycolic acid nanoparticles for dendritic cell-based targeted anticancer therapy.
abstract: BACKGROUND
  Delivery of PLGA (poly [D, L-lactide-co-glycolide])-based biodegradable nanoparticles (NPs) to antigen presenting cells, particularly dendritic cells, has potential for cancer immunotherapy.
  
  
  METHODS
  Using a PLGA NP vaccine construct CpG-NP-Tag (CpG-ODN-coated tumor antigen [Tag] encapsulating NP) prepared using solvent evaporation technique we tested the efficacy of ex vivo and in vivo use of this construct as a feasible platform for immune-based therapy.
  
  
  RESULTS
  CpG-NP-Tag NPs were avidly endocytosed and localized in the endosomal compartment of bone marrow-derived dendritic cells. Bone marrow-derived dendritic cells exposed to CpG-NP-Tag NPs exhibited an increased maturation (higher CD80/86 expression) and activation status (enhanced IL-12 secretion levels). In vivo results demonstrated attenuation of tumor growth and angiogenesis as well as induction of potent cytotoxic T-lymphocyte responses.
  
  
  CONCLUSIONS
  Collectively, results validate dendritic cells stimulatory response to CpG-NP-Tag NPs (ex vivo) and CpG-NP-Tag NPs' tumor inhibitory potential (in vivo) for therapeutic applications, respectively.
authors.affiliation: Department of Molecular & Medical Genetics, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.|Institute of Cancer Research, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.
authors.forename: Rutika A
authors.initials: RA
authors.lastname: Kokate
authors.affiliation: Department of Molecular & Medical Genetics, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.|Institute of Cancer Research, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Chaudhary
authors.affiliation: Department of Cell Biology & Immunology, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.
authors.forename: Xiangle
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Molecular & Medical Genetics, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.|Institute of Cancer Research, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.|RadioMedix Inc., 9701 Richmond Avenue, Suite 222, Houston, TX 77042, USA.
authors.forename: Sanjay I
authors.initials: SI
authors.lastname: Thamake
authors.affiliation: Department of Molecular & Medical Genetics, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.|Institute of Cancer Research, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.
authors.forename: Sayantan
authors.initials: S
authors.lastname: Maji
authors.affiliation: Department of Cell Biology & Immunology, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.
authors.forename: Rahul
authors.initials: R
authors.lastname: Chib
authors.affiliation: Department of Molecular & Medical Genetics, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.|Institute of Cancer Research, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.
authors.forename: Jamboor K
authors.initials: JK
authors.lastname: Vishwanatha
authors.affiliation: Department of Molecular & Medical Genetics, University of North Texas Health Science Center (UNTHSC), 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.|Institute of Cancer Research, University of North Texas Health Science Center (UNTHSC), Fort Worth, TX 76107, USA.
authors.forename: Harlan P
authors.initials: HP
authors.lastname: Jones
mesh_terms: D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004705:Endocytosis / Q000276:immunology; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry; D058990:Molecular Targeted Therapy; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology
chemical_list: D019496:Cancer Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: DCs; NP; breast cancer; cancer vaccines; dendritic cells; immunotherapy; nanoparticle