title: Surface Functionalization of Titanium Alloy with miR-29b Nanocapsules To Enhance Bone Regeneration.
abstract: Titanium and its alloys have been widely used over the past 3 decades as implants for healing bone defects. Nevertheless, the bioinert property of titanium alloy limits its clinical application and surface modification method is frequently performed to improve the biological and chemical properties. Recently, the delivery of microRNA with osteogenesis capability has been recognized as a promising tool to enhance bone regeneration of implants. Here, we developed a biodegradable coating to modify the titanium surface in order to enhance osteogenic bioactivity. The previous developed nanocapsules were used as the building blocks, and then a bioactive titanium coating was designed to entrap the miR-29b nanocapsules. This coating was not only favorable for cell adhesion and growth but also provided sufficient microRNA transfection efficacy and osteoinductive potential, resulting in a significant enhancement of bone regeneration on the surface of bioinert titanium alloy.
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Department of Clinical Microbiology, School of Laboratory Medicine, Tianjin Medical University , Tianjin 300203, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Chaoyong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Microbiology, School of Laboratory Medicine, Tianjin Medical University , Tianjin 300203, China.
authors.forename: Yunde
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Xubo
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Zhenduo
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science & Engineering, Tianjin University , Tianjin 300072, China.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000497:Alloys / Q000737:chemistry*; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D053769:Nanocapsules / Q000737:chemistry* / Q000633:toxicity; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D013977:Tibia / Q000502:physiology; D014025:Titanium / Q000737:chemistry*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D000497:Alloys; D050659:Core Binding Factor Alpha 1 Subunit; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D053769:Nanocapsules; D015675:Osteocalcin; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: bone regeneration; miR-29b; nanocapsules; osteogenic bioactivity; titanium