title: Expression of CDc6 after acute spinal cord injury in adult rats.
abstract: The cell division cycle 6 (CDc6) protein has been primarily investigated as a component of the pre-replicative complex for the initiation of DNA replication. Some studies have shown that CDc6 played a critical role in the development of human carcinoma. However, the expression and roles of CDc6 in the central nervous system remain unknown. We have performed an acute spinal cord injury (SCI) model in adult rats and investigated the dynamic changes of CDc6 expression in spinal cord. Western blot have found that CDc6 protein levels first significantly increase, reach a peak at day 3, and then gradually return to normal level at day 14 after SCI. Double immunofluorescence staining showed that CDc6 immunoreactivity was found in neurons, astrocytes, and microglia. Additionally, colocalization of CDc6/active caspase-3 has been detected in neurons and colocalization of CDc6/proliferating cell nuclear antigen has been detected in astrocytes and microglial. In vitro, CDc6 depletion by short interfering RNA inhibits astrocyte proliferation and reduces cyclin A and cyclin D1 protein levels. CDc6 knockdown also decreases neuronal apoptosis. We speculate that CDc6 might play crucial roles in CNS pathophysiology after SCI.
authors.affiliation: Department of orthopedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Neurology, Nantong Second People's Hospital, Nantong 226001, Jiangsu Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Medical image, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, Shanghai Changzheng Hospital, 200000 Shanghai, China.
authors.forename: Jian-Ru
authors.initials: JR
authors.lastname: Xiao
authors.affiliation: Department of Orthopedics, Shanghai Changzheng Hospital, 200000 Shanghai, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Wei
authors.affiliation: Department of Neurology, Nantong Second People's Hospital, Nantong 226001, Jiangsu Province, China.
authors.forename: Xin-jian
authors.initials: XJ
authors.lastname: Song
authors.affiliation: Department of Neurology, Nantong Second People's Hospital, Nantong 226001, Jiangsu Province, China.
authors.forename: Jian-Bing
authors.initials: JB
authors.lastname: Ge
authors.affiliation: Department of Neurology, Nantong Second People's Hospital, Nantong 226001, Jiangsu Province, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tao
authors.affiliation: Department of orthopedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qian
authors.affiliation: Department of Orthopedic Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 200233 Shanghai, China. Electronic address: njh306@163.com.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Yu
authors.affiliation: Department of Orthopedics, Shanghai Changzheng Hospital, 200000 Shanghai, China. Electronic address: drzhaojian123@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D019925:Cyclin A / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism*
chemical_list: C528180:Cdc6 protein, rat; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D019925:Cyclin A; D018809:Proliferating Cell Nuclear Antigen; D019938:Cyclin D1; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: CDc6; Cell cycle; Neuronal apoptosis; Proliferation; Spinal cord injury