title: Signal-on electrochemical detection of antibiotics at zeptomole level based on target-aptamer binding triggered multiple recycling amplification.
abstract: In the work, a signal-on electrochemical DNA sensor based on multiple amplification for ultrasensitive detection of antibiotics has been reported. In the presence of target, the ingeniously designed hairpin probe (HP1) is opened and the polymerase-assisted target recycling amplification is triggered, resulting in autonomous generation of secondary target. It is worth noting that the produced secondary target could not only hybridize with other HP1, but also displace the Helper from the electrode. Consequently, methylene blue labeled HP2 forms a "close" probe structure, and the increase of signal is monitored. The increasing current provides an ultrasensitive electrochemical detection for antibiotics down to 1.3 fM. To our best knowledge, such work is the first report about multiple recycling amplification combing with signal-on sensing strategy, which has been utilized for quantitative determination of antibiotics. It would be further used as a general strategy associated with more analytical techniques toward the detection of a wide spectrum of analytes. Thus, it holds great potential for the development of ultrasensitive biosensing platform for the applications in bioanalysis, disease diagnostics, and clinical biomedicine.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Resources and Environment, University of Jinan, Jinan 250022, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yuna
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China; College of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China. Electronic address: chm_huangjd@ujn.edu.cn.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Huang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D057230:Limit of Detection; D008751:Methylene Blue / Q000737:chemistry; D009693:Nucleic Acid Hybridization
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D008751:Methylene Blue
keywords: Antibiotics detection; Multiple amplification; Signal-on sensor; Zeptomole determination