title: Synthesis and characterization of anti-bacterial and anti-fungal citrate-based mussel-inspired bioadhesives.
abstract: Bacterial and fungal infections in the use of surgical devices and medical implants remain a major concern. Traditional bioadhesives fail to incorporate anti-microbial properties, necessitating additional anti-microbial drug injection. Herein, by the introduction of the clinically used and inexpensive anti-fungal agent, 10-undecylenic acid (UA), into our recently developed injectable citrate-based mussel-inspired bioadhesives (iCMBAs), a new family of anti-bacterial and anti-fungal iCMBAs (AbAf iCs) was developed. AbAf iCs not only showed strong wet tissue adhesion strength, but also exhibited excellent in vitro cyto-compatibility, fast degradation, and strong initial and considerable long-term anti-bacterial and anti-fungal ability. For the first time, the biocompatibility and anti-microbial ability of sodium metaperiodate (PI), an oxidant used as a cross-linking initiator in the AbAf iCs system, was also thoroughly investigated. Our results suggest that the PI-based bioadhesives showed better anti-microbial properties compared to the unstable silver-based bioadhesive materials. In conclusion, AbAf iCs family can serve as excellent anti-bacterial and anti-fungal bioadhesive candidates for tissue/wound closure, wound dressing, and bone regeneration, especially when bacterial or fungal infections are a major concern.
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA; Zhejiang Provincial Top Key Discipline of Bioengineering, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA; School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Academy of Orthopedics of Guangdong Province, Guangzhou 510630, China.
authors.forename: Denghui
authors.initials: D
authors.lastname: Xie
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Ethan
authors.initials: E
authors.lastname: Gerhard
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Merisa
authors.initials: M
authors.lastname: Nisic
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Dingying
authors.initials: D
authors.lastname: Shan
authors.affiliation: Zhejiang Provincial Top Key Discipline of Bioengineering, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Qian
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Siyang
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA. Electronic address: jxy30@psu.edu.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D000935:Antifungal Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry; D049872:Bivalvia / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019343:Citric Acid / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D020100:Hydrogels; D009682:Magnetic Resonance Spectroscopy; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014014:Tissue Adhesives / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001672:Biocompatible Materials; D020100:Hydrogels; D014014:Tissue Adhesives; D019343:Citric Acid; D012834:Silver
keywords: Antibacterial; Antifungal; Citric acid; Silver nanoparticles; Sodium metaperiodate; Wound closure