title: Quantitative PCR studies of Aggregatibacter actinomycetemcomitans and Treponema denticola/Tanerella forsythensis Complex as Etiological Factors of Chronic Periodontitis.
abstract: Real-time quantitative PCR (Dentofl or kit) was used to detect DNA of periodontal pathogens in specimens from 92 patients with chronic periodontitis and from a control sample of 12 normal subjects. A bimodal distribution of patients by periodontium colonization with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythensis, and T. denticola was demonstrated. A new approach to interpretation of the results of quantitative evaluation of periodontal pathogens, including the notion of pathological colonization level, led to classification of all cases with chronic generalized periodontitis into 3 groups: associated with A. actinomycetemcomitans, with T. forsythensis/T. denticola complex, and cases of uncertain genesis.
authors.affiliation: A. I. Evdokimov Moscow State University of Medicine and Dentistry, the Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: O O
authors.initials: OO
authors.lastname: Yanushevich
authors.affiliation: A. I. Evdokimov Moscow State University of Medicine and Dentistry, the Ministry of Health of the Russian Federation, Moscow, Russia. a_reg@mail.ru.
authors.forename: R A
authors.initials: RA
authors.lastname: Ayvazova
authors.affiliation: N. M. Emanuel Institute of Biochemical Physics, the Russian Academy of Sciences, Moscow, Russia.|M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Shibaeva
authors.affiliation: N. I. Vavilov Institute of Common Genetics, the Russian Academy of Sciences, Moscow, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Rebrikov
authors.affiliation: Kursk State University, Kursk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Trubnikova
authors.affiliation: M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Yu K
authors.initials: YK
authors.lastname: Kudykina
authors.affiliation: M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences, Moscow, Russia.|Naberezhnye Chelny Institute of Socio-Pedagogical Technologies and Resources, Naberezhnye Chelny, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Zylnikova
authors.affiliation: Naberezhnye Chelny Institute of Socio-Pedagogical Technologies and Resources, Naberezhnye Chelny, Russia.
authors.forename: R S
authors.initials: RS
authors.lastname: Zaripova
authors.affiliation: M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Shevelev
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification*; D055113:Chronic Periodontitis / Q000382:microbiology*; D006801:Humans; D008875:Middle Aged; D010519:Periodontium / Q000382:microbiology* / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D000070436:Tannerella forsythia / Q000302:isolation & purification*; D048373:Treponema denticola / Q000302:isolation & purification*; D055815:Young Adult
keywords: Aggregatibacter actinomycetemcomitans; Dentoflor; Tanerella forsythensis; Treponema denticola; periodontitis