title: Expression of IL-1 and IL-6 and their natural regulators in leukocytes of B-cell chronic lymphocytic leukaemia patients.
abstract: OBJECTIVE
  The purpose of the study was the assessment of the expression of IL-1β and IL-6, and the proteins regulating their biological activity, namely IL-1RII, IL-1Ra, as well as sIL-6Rα, sgp-130 in leukemic lymphocytes and autologous neutrophils of B-CLL patients.
  
  
  METHODS
  The study involved a group of B-cell chronic lymphocytic leukemia patients and healthy volunteer blood donors. The presence of chosen proteins and their natural regulators was confirmed by Western blot.
  
  
  RESULTS
  Western blot analysis showed a decreased expression of IL-1β and IL-6 in the leukocytes of B-CLL patients. Decreased expression of sIL-6Rα has been observed in lymphocytes, with a simultaneous increase of expression in PMNs. Lower expression of sgp-130 was found in B cells while its expression was elevated in the neutrophils of patients in early stages of the disease.
  
  
  CONCLUSIONS
  The changes observed in the expression of IL-1 and IL-6 seem to exclude their immediate involvement in the progress of B-CLL. However, the presented changes in the expression of proteins regulating IL-1β and IL-6 in PMNs indicate a potential role of early immune response cells also in advanced stages of the disease.
authors.affiliation: Department of Immunology, Medical University of Bialystok, Bialystok, Poland. Electronic address: marzena.garley@umb.edu.pl.
authors.forename: Marzena
authors.initials: M
authors.lastname: Garley
authors.affiliation: Department of Immunology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Jabłońska
authors.affiliation: Department of Family Medicine and Community Nursing, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Sawicka-Powierza
authors.affiliation: Department of Haematology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Kłoczko
authors.affiliation: Department of Haematology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Piszcz
authors.affiliation: Department of Immunology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kakareko
authors.affiliation: Department of Immunology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Wioletta
authors.initials: W
authors.lastname: Ratajczak-Wrona
authors.affiliation: Department of Public Health, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Angelika
authors.initials: A
authors.lastname: Charkiewicz
authors.affiliation: Department of Public Health, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Wioleta
authors.initials: W
authors.lastname: Omeljaniuk
authors.affiliation: Department of Public Health, Medical University of Bialystok, Bialystok, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szpak
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6
keywords: B-CLL; B-lymphocytes; Interleukins; Neutrophils