title: TLR2 expression doesn't change in ox-LDL mediated inflammation in Human umbilical vein endothelial cells under high glucose culture.
abstract: BACKGROUND
  Inflammatory responses induced by ox-LDL play important roles in atherogenesis, and could be promoted in diabetic patients. Toll-like receptor (TLR)2 is an innate inflammatory receptor, and is enhanced in human umbilical vein endothelial cells (HUVECs) under high glucose conditions. Ox-LDL-TLR2 pathway activation and further inflammation in monocytes are involved in the atherosclerosis formation.
  
  
  OBJECTIVE
  What role of TLR2 plays on ox-LDL-induced inflammation in HUVECs remains unclear, especially in high glucose conditions. The purpose of this study is to explore the effect and role of ox-LDL-TLR2 pathway on the inflammatory responses in HUVECs.
  
  
  METHODS
  1 hour prior to the treatment, HUVECs were treated with or without neutralizing anti-TLR2 antibody. After that, HUVECs were treated with ox-LDL (20, or 40 μg/ml) or LPS (200 ng/ml) under normal and high glucose conditions. The expressions of ICAM-1 and TLR2 protein were analyzed by immunoblotting, and IL-6 and IL-8 were measured by ELISA.
  
  
  RESULTS
  Compared with those in normal glucose condition, IL-6 and IL-8 expression were increased in high glucose condition. The stimulation of ox-LDL and LPS both increased the expression of ICAM-1, IL-6 and IL-8, but did not change TLR2 protein expression in both normal and high glucose conditions. Additionally, the expression of ICAM-1, IL-6 and IL-8 was not changed when TLR2 was knocked out under these two conditions.
  
  
  CONCLUSIONS
  The inflammatory responses induced by Ox-LDL were not changed with or without TLR2 under both normal and high glucose conditions in HUVECs. Our study indicates TLR2 is not involved in the ox-LDL mediated endothelial injury under high glucose conditions, which is an important step of atherosclerosis formation in diabetes.
authors.affiliation: Department of Interventional cardiology, First Affiliated Hospital of Shantou University Medical College Shantou, Guangdong Province, China.
authors.forename: Jilin
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Interventional cardiology, First Affiliated Hospital of Shantou University Medical College Shantou, Guangdong Province, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Plastic Surgeon, First Affiliated Hospital of Shantou University Medical College Shantou, Guangdong Province, China.
authors.forename: Xiangna
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Cardiology, Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong Province, China.
authors.forename: Huaiwen
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Interventional cardiology, First Affiliated Hospital of Shantou University Medical College Shantou, Guangdong Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Second Affiliated Hospital of Shantou University Medical College Shantou, Guangdong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
keywords: HUVECs; TLR2; inflammation; ox-LDL