title: How erosive drinks and enzyme inhibitors impact bond strength to dentin.
abstract: Concern has been raised about the bonding of restorative procedures to an erosive lesion, given the change in organic and inorganic composition and structure of this substrate. This in vitro study evaluated the effect of erosive drinks and an enzyme inhibitor (2% chlorhexidine digluconate - 2% CHX) on bond strength to dentin. Sixty sound human third molars were selected, and the occlusal enamel was flattened, exposing the dentin surface. The specimens were randomly divided into three groups: AS-Artificial saliva (control group), RC- Regular Cola and ZC- Zero Cola. Twenty specimens were immersed in their respective solution for 1 minute, 3 times a day, over the course of 5 days. After acid etching and before bonding with Adper Single Bond 2, half of the samples of each group (n = 10) were treated with 2% CHX, whereas the other half (n = 10) were not, forming the control group (CONV). All the specimens were restored with Filtek Z250 composite resin filled in Tygon tubes (0.48 mm2), yielding six microcylinders for microshear bond strength testing. Three composite resin microcylinders of each specimen were tested after 1 month, and the remaining microcylinders were tested after 6 months. Failure modes were determined using a stereomicroscope (40x). The data were statistically analyzed by three-way ANOVA and Tukey tests (α = 0.05). Overall bonding was reduced after 6 months, regardless of treatment. The 2% CHX enhanced bond strength after 1 month only in the ZC group, and did not enhance bonding performance to demineralized dentin by erosive protocol after 6 months in any group.
authors.affiliation: Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Camila Moreira
authors.initials: CM
authors.lastname: Machado
authors.affiliation: Jundiaí, Jundiaí, SP, Brazil.
authors.forename: Aline Cristina
authors.initials: AC
authors.lastname: Zamuner
authors.affiliation: School of Dentistry, Universidade do Sagrado Coração, Bauru, SP, Brazil.
authors.forename: Karin Cristina da Silva
authors.initials: KC
authors.lastname: Modena
authors.affiliation: Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Sérgio Kiyoshi
authors.initials: SK
authors.lastname: Ishikiriama
authors.affiliation: Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Linda
authors.initials: L
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000737:chemistry; D002253:Carbonated Beverages; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000737:chemistry*; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods; D003738:Dental Cements / Q000737:chemistry; D003743:Dental Enamel / Q000187:drug effects; D019232:Dental Restoration Failure; D003804:Dentin / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry*; D006801:Humans; D007101:Immersion; D066298:In Vitro Techniques; D011897:Random Allocation; D012464:Saliva, Artificial / Q000737:chemistry; D033081:Shear Strength / Q000187:drug effects; D013499:Surface Properties / Q000187:drug effects; D013997:Time Factors; D014077:Tooth Erosion / Q000139:chemically induced*
chemical_list: C554641:Adper single bond 2; D000891:Anti-Infective Agents, Local; D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D004791:Enzyme Inhibitors; C444701:Filtek Z250; D012464:Saliva, Artificial; C010882:chlorhexidine gluconate; D002710:Chlorhexidine