title: Concentration of glycosaminoglycan in ovariectomized mice uterus after treatment with ovarian steroids.
abstract: The aim of this study was to evaluate the amount of non- and sulfated glycosaminoglycans in the ovariectomized mice uterus, after treatment with ovarian steroids. For this purpose, 50 adult female mice were divided into five groups with 10 animals/each: control group: CG (ovary intact), and ovariectomized groups: OG (vehicle), EG (estradiol), PG (progesterone) and EPG (estradiol combined to progesterone). The treatments started 30 days after ovariectomy. All the animals were treated for 50 consecutive days. These hormones were administered in a sterile oily solution via gavage. Twenty-four hours after the last treatment, all animals were euthanized, removing the uterine horn for biochemical analyses. To quantify, the hyaluronic acid (HA) used ELISA-like fluorometric assay, and the sulfated glycosaminoglycans (GAGs) used agarose gel electrophoresis. The amount of HA was significantly higher in the group treated with progesterone (PG) compared to the others groups (p < 0.05), and in the group treated with estradiol (EG), the amount of chondroitin/dermatan sulfate was significantly higher compared to the others groups (p < 0.05), and in the group treated with progesterone (PG), the amount of heparan sulfate was significantly lower compared to the others groups, except to control group (p < 0.05). Our results showed that the estroprogestative therapy after long time (50 days) profoundly affected the amount of glycosaminoglycans in uterine. These changes may be indicative of uterine pathology such as the development of tumor.
authors.affiliation: a Department of Gynecology , Universidade Federal de São Paulo , Brazil.
authors.forename: Gabriela C C C
authors.initials: GC
authors.lastname: Maioral
authors.affiliation: a Department of Gynecology , Universidade Federal de São Paulo , Brazil.|b Department of Morphology and Genetics , Universidade Federal de São Paulo , Brazil.
authors.forename: Regina Célia T
authors.initials: RC
authors.lastname: Gomes
authors.affiliation: a Department of Gynecology , Universidade Federal de São Paulo , Brazil.
authors.forename: Carina
authors.initials: C
authors.lastname: Verna
authors.affiliation: a Department of Gynecology , Universidade Federal de São Paulo , Brazil.|b Department of Morphology and Genetics , Universidade Federal de São Paulo , Brazil.
authors.forename: Manuel de J
authors.initials: MJ
authors.lastname: Simões
authors.affiliation: d Department of Biochemistry , Molecular Biology Division, Universidade Federal de São Paulo , Brazil.
authors.forename: Helena B
authors.initials: HB
authors.lastname: Nader
authors.affiliation: c Department of Obstetrics and Gynecology , Gynecology Division, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo , Brazil , and.
authors.forename: Ricardo S
authors.initials: RS
authors.lastname: Simões
authors.affiliation: c Department of Obstetrics and Gynecology , Gynecology Division, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo , Brazil , and.
authors.forename: Edmund C
authors.initials: EC
authors.lastname: Baracat
authors.affiliation: c Department of Obstetrics and Gynecology , Gynecology Division, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo , Brazil , and.
authors.forename: José Maria
authors.initials: JM
authors.lastname: Soares
mesh_terms: D000818:Animals; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D012739:Gonadal Steroid Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D010052:Ovariectomy; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D012739:Gonadal Steroid Hormones; D011374:Progesterone; D004958:Estradiol
keywords: Hyaluronic acid; ovarian steroids; sulfated glycosaminoglycans