title: Circulating "LncPPARδ" From Monocytes as a Novel Biomarker for Coronary Artery Diseases.
abstract: To investigate long noncoding RNA NONHSAT112178 (LncPPARδ) as a biomarker for coronary artery disease (CAD) in peripheral blood monocyte cells, RT-qPCR was performed to validate the microarray results, receiver operating characteristic curve was applied to study the potential of LncPPARδ as a biomarker. Diagnostic models from LncPPARδ alone or combination of risk factors were constructed by Fisher criteria. The expression of genes neighboring the LncPPARδ gene was examined with RT-qPCR in THP-1 cell line treated with LncPPARδ siRNA. Using a diagnostic model by Fisher criteria, the consideration of risk factors increased the optimal sensitivity from 70.00% to 82.00% and decreased the specificity from 94.00% to 78.00%. The consideration of risk factors also increased area under the receiver operating characteristic curve from 0.727 to 0.785 (P = 0.001), from 0.712 to 0.768 (P = 0.01), and from 0.769 to 0.835 (P = 0.07), in the original, training, and test sets, respectively. Finally, we found that the expression of peroxisome proliferator-activated receptor δ (PPARδ), Adipose Differentiation-Related Protein (ADRP), and Angiopoietin-like 4 (ANGPTL4) were affected by LncPPARδ silencing.Our present study indicated that LncPPARδ, especially combined with risk factors, can be a good biomarker for CAD. LncPPARδ regulates the expression of neighboring protein-coding genes, PPARδ and its direct target genes ADRP and ANGPTL4.
authors.affiliation: From the Department of Cardiology, Daping Hospital, The Third Military Medical University (YC, YY, XC, DH, XZ, XW, JH, CF, DQ, CZ, LZ); Chongqing Institute of Cardiology (YC, YY, XC, DH, XZ, XW, JH, CF, DQ, CZ, LZ); Department of Neurology, Daping Hospital, The Third Military Medical University (YC, YY), Chongqing, P.R. China; Cardiovascular Research Center and Department of Physiology, Temple University School of Medicine, Philadelphia, PA (XC); Division of Nephrology, Department of Medicine (PAJ) and Department of Physiology (PAJ), University of Maryland School of Medicine, Baltimore, MD.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cai
authors.forename: Yujia
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiongwen
authors.initials: X
authors.lastname: Chen
authors.forename: Duofeng
authors.initials: D
authors.lastname: He
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiulan
authors.initials: X
authors.lastname: Wen
authors.forename: Jiayong
authors.initials: J
authors.lastname: Hu
authors.forename: Chunjiang
authors.initials: C
authors.lastname: Fu
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Qiu
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Jose
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zeng
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D047494:PPAR delta / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000097:blood*; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D047494:PPAR delta; D062085:RNA, Long Noncoding