title: Beta-lactamase Enzymes of ClinicalPseudomonas aeruginosaStrains.
abstract: OBJECTIVE
  In this study, the production of extended spectrum beta-lactamase (ESBL), metallo-betalacatamase (MBL) and AmpC beta-lactamase enzymes ofPseudomonas aeruginosa (P aeruginosa)strains which were isolated from clinical samples were investigated.AmpCgene was also detected by the polymerase chain reaction (PCR) analysis.
  
  
  METHODS
  A hundred strains ofP aeruginosawere included in the study. The presence of ESBL was investigated with combined disk confirmation test, MBL was investigated with E-test method and AmpC beta-lactamase was investigated with disk induction test. In order to detect the production of AmpC betalactamase genotypically, the PCR method was used.
  
  
  RESULTS
  Only one strain was found to be MBL positive. Four per cent of strains were found to be ESBL positive. AmpC beta-lactamase production was positive in 73% of the strains with disk induction test.AmpCgene was detected in 96% of the studied strains with the PCR method.
  
  
  CONCLUSIONS
  While ESBL and MBL rates in this study were significantly lower than those found in other studies, the rate of AmpC beta-lactamase was higher. AlthoughAmpCgene was detected in some strains (23%), they were not found to produce AmpC beta-lactamase with disk induction test.
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Medical Microbiology, Hatay, Turkey.
authors.forename: O
authors.initials: O
authors.lastname: Pasa
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Medical Microbiology, Hatay, Turkey. E-mail: burcinozer@yahoo.com.
authors.forename: B
authors.initials: B
authors.lastname: Ozer
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Medical Microbiology, Hatay, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Duran
authors.affiliation: Mustafa Kemal University, School of Medicine, Department of Medical Microbiology, Hatay, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Inci
authors.affiliation: Katip Celebi University, School of Medicine, Department of Medical Microbiology, Izmir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Yula
keywords: AmpC beta-lactamase; AmpC gene; Pseudomonas aeruginosa; extended spectrum beta-lactamase; metallo-beta-lactamase; resistance