title: A novel aptasensor for electrochemical detection of ractopamine, clenbuterol, salbutamol, phenylethanolamine and procaterol.
abstract: β-agonists are phenylethanolamines with different substituent groups on the aromatic ring and the terminal amino group which have the effect of nutrition redistribution and can accumulate in body tissues causing acute or chronic poisoning when consumed. Therefore, it is very important to establish a fast screening method for the detection of several kinds of β-agonists in food safety control. In this study, the aptamer-agonists (AP-Ago) has screened out by Isothermal Titration Calorimetric method. AP-Ago was a single-strand DNA with 22 base-pairs. The dissociation constant (Kd) to phenylethanolamine (PHL) was 3.34 × 10(-5)mol L(-1). The AP-Ago based electrode was constructed by self-assembling on gold electrode. A label-free electrochemical aptasensor was then developed with AP-Ago-based gold electrode, which was sensitive to phenylethanolamine(PHL), clenbuterol (CLB), ractopamine (RAC), salbutamol (SAL) and procaterol (PRO). The detection limits were 0.04 ng/mL (RAC), 0.35 pg/mL (CLB), 1.0 pg/mL (PHL), 0.53 pg/mL (SAL) and 1.73 pg/mL(PRO), respectively, The detection time was 15 min. The reproductivity of the mentioned aptasensor is good with RSD of 2.09%. Comparing with ELISA and HPLC on β-agonists detection in actual sample, this aptasensor is advantage of fewer steps and fast screen-detection of these five β-agonists or their mixtures. This study suggests that the aptasensor can be developed to a rapid screening means with multi-β-agonists (may be one or more) in sample.
authors.affiliation: Institute of Microbial Technology, Jinan University, Guangzhou 510632, China; National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Institute of Microbial Technology, Jinan University, Guangzhou 510632, China; College of life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Institute of Microbial Technology, Jinan University, Guangzhou 510632, China; National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Nianjue
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Institute of Microbial Technology, Jinan University, Guangzhou 510632, China; College of life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Xie
authors.affiliation: Institute of Microbial Technology, Jinan University, Guangzhou 510632, China; College of life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Daling
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Microbial Technology, Jinan University, Guangzhou 510632, China; College of life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Microbial Technology, Jinan University, Guangzhou 510632, China; National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China. Electronic address: tdsyao@jnu.edu.cn.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Yao
mesh_terms: D000420:Albuterol / Q000302:isolation & purification; D052157:Aptamers, Nucleotide; D015374:Biosensing Techniques; D002976:Clenbuterol / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000737:chemistry; D055664:Electrochemical Techniques; D005504:Food Analysis; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D010627:Phenethylamines / Q000302:isolation & purification; D017265:Procaterol / Q000302:isolation & purification
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D010627:Phenethylamines; C060897:ractopamine; D006046:Gold; D000420:Albuterol; D017265:Procaterol; D002976:Clenbuterol
keywords: Aptasensor; Electrochemical detection; Selectivity; β-agonists