title: Fertility defects in Surfactant associated protein D knockout female mice: altered ovarian hormone profile.
abstract: Surfactant associated protein D (SFTPD, also known as SP-D), a pattern recognition molecule, is an integral component of the mucosal immune system of female reproductive tract (FRT). In addition to host defense functions in the FRT, recent evidences indicate immunomodulatory role of SFTPD in parturition and pre-term labor. Regulation of SFTPD expression by ovarian hormones in the mouse uterus implicates SFTPD of FRT in pregnancy establishment and maintenance. In the current study, we attempted to decipher the functional relevance of SFTPD in FRT by characterizing the fertility parameters of surfactant associated protein D knockout (Sftpd(tm1Jhf)/Sftpd(tm1Jhf)) female mice. Knockout female mice exhibited extended estrous cycle with altered serum profile of ovarian hormones. We also demonstrate altered expression of ovarian hormone receptors and hormone responsive genes ITGB1, LIF and HOXA10 in uteri of these mice. Knockout females mated with wild type males had significantly smaller litter size due to increased pre-implantation embryo loss. We also observed an altered immune profile in knockout mice uteri with elevated levels of inflammatory cytokines, increased numbers of pro-inflammatory monocytes/macrophages and lower FOXP3 levels during the pre-implantation period. LPS administration to pregnant knockout mice did not result in any increase in embryo implantation loss and was associated with a blunted uterine pro-inflammatory response, plausibly due to higher levels of serum progesterone. Taken together, our results demonstrate that SFTPD deficiency affects female fertility, highlighting roles for SFTPD in ovarian and uterine physiology.
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health (ICMR), Mumbai, Maharashtra, India. Electronic address: kay_sharon64@yahoo.co.in.
authors.forename: Sharon
authors.initials: S
authors.lastname: Kay
authors.affiliation: Department of Innate Immunity, National Institute for Research in Reproductive Health (ICMR), Mumbai, Maharashtra, India. Electronic address: taruna_m@hotmail.com.
authors.forename: Taruna
authors.initials: T
authors.lastname: Madan
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020964:Embryo Loss / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005298:Fertility / Q000276:immunology*; D005434:Flow Cytometry; D042341:Gonadal Hormones / Q000276:immunology*; D018928:Immunity, Mucosal / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D018345:Mice, Knockout; D010053:Ovary / Q000276:immunology; D011247:Pregnancy; D037663:Pulmonary Surfactant-Associated Protein D / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction; D014599:Uterus / Q000276:immunology
chemical_list: D042341:Gonadal Hormones; D037663:Pulmonary Surfactant-Associated Protein D
keywords: LPS; Macrophage; Ovarian hormone; Pre-implantation embryo loss; SFTPD; Uterus