title: A unique macrolactam derivative via a [4+6]-cycloaddition from Streptomyces niveus.
abstract: One new macrolactam derivative, nivelactam (1) and one new polyenoic acid derivative, niveamide (2), along with two other known 20-atom macrolactams (3 and 4) were isolated from the fermentation broth of Streptomyces niveus, which obtained from the forest soil in northeastern China. The structures of 1 and 2 were elucidated on the basis of HRESIMS, IR, and NMR spectroscopic data analyses. Compound 1 was proposed as an intramolecular [4+6]-cycloaddition product of 3 by S. niveus, and displayed moderate cytotoxic activity against a panel of human tumor cell lines in vitro, with IC50 values ranging from 3.76 ± 0.58 to 15.02 ± 2.81 μM.
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Liya
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China. Electronic address: hanli@mail.neu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
authors.forename: Xueshi
authors.initials: X
authors.lastname: Huang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D061565:Cycloaddition Reaction; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D047029:Lactams, Macrocyclic / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D015394:Molecular Structure; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D047029:Lactams, Macrocyclic
keywords: Cycloaddition; Cytotoxicity; In vitro; Macrolactam; Streptomyces niveus