title: Evaluation of Porcine Myofibrillar Protein Gel Functionality as Affected by Microbial Transglutaminase and Red Bean [Vignia angularis] Protein Isolate at Various pH Values.
abstract: This study was investigated to determine the effect of microbial transglutaminase (MTG) with or without red bean protein isolate (RBPI) on the porcine myofibrillar protein (MP) gel functionality at different pH values (pH 5.75-6.5). Cooking yield (CY, %), gel strength (GS, gf), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) were determined to measure gel characteristics. Since no differences were observed the interaction between 1% RBPI and pH, data were pooled. CY increased with the addition of 1% RBPI, while it was not affected by pH values. GS increased with increased pH and increased when 1% RBPI was added, regardless of pH. There were distinctive endothermic protein peaks, at 56.55 and 75.02℃ at pH 5.75, and 56.47 and 72.43℃ at pH 6.5 in DSC results, which revealed decreased temperature of the first peak with the addition of 1% RBPI and increased pH. In SEM, a more compact structure with fewer voids was shown with the addition of 1% RBPI and increased pH from 5.75 to 6.5. In addition, the three-dimensional structure was highly dense and hard at pH 6.5 when RBPI was added. These results indicated that the addition of 1% RBPI at pH 6.5 in MTG-mediated MP represent the optimum condition to attain maximum gel-formation and protein gel functionality.
authors.affiliation: Department of Animal Science and Functional Foods Research Center, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Ho Sik
authors.initials: HS
authors.lastname: Jang
authors.affiliation: Department of Animal Science and Functional Foods Research Center, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Hong Chul
authors.initials: HC
authors.lastname: Lee
authors.affiliation: Department of Animal Science and Functional Foods Research Center, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Koo Bok
authors.initials: KB
authors.lastname: Chin
keywords: microbial transglutaminase; myofibrillar protein gel functionality; pH; red bean protein isolate