title: Antioxidant activities and liquid chromatography with electrospray ionization tandem mass spectrometry characterization and quantification of the polyphenolic contents of Rumex nervosus Vahl leaves and stems.
abstract: In the present study, four compounds, viz. chlorogenic acid, catechin, orientin, and apigenin-O-acetylglycoside among 18 polyphenol compounds (17 flavonoids and one hydroxycinnamic acid derivative) were characterized for the first time in Rumex nervosus leaves and stems by using liquid chromatography with electrospray ionization tandem mass spectrometry. Method validation in terms of determination coefficient, limits of detection, and quantification were ≥ 0.9979, 0.68-1.61, and 2.27-5.38 mg/L, respectively. Accuracy, expressed as percent recovery for two spiking levels (10 and 50 mg/L), were in the range 78.9-110.6% with the exception of caffeic acid. The relative standard deviations were 1-17%. The total polyphenol content was higher by approximately two times in the leaf (1073 mg/kg fresh sample) than in the stem (519.86 mg/kg fresh sample). The antioxidant effects increased in a dose-dependent manner, and the scavenging activities, investigated by measuring 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity, ferrous ions chelating activity, superoxide anion radical scavenging activity, and ferric reducing antioxidant power activity, were significant (p < 0.05) using low concentrations of the leaf extract. Overall, the present study suggests that different parts of R. nervosus have great potential for producing a range of extracts with potential applications in medicine.
authors.affiliation: Department of Chemistry, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Kebede Taye
authors.initials: KT
authors.lastname: Desta
authors.affiliation: Department of Internal Medicine, Gyeongnam Regional Cancer Center, Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Won Sup
authors.initials: WS
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Sung Joong
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Yun-Hi
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Gon-Sup
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Department of Food and Nutrition, Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Information Sciences, Research Institute of Natural Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Soo Taek
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Kwangjin-gu, Seoul, Republic of Korea.|Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.affiliation: Biochemistry and Chemistry of Nutrition Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Warda
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Kwangjin-gu, Seoul, Republic of Korea.
authors.forename: Ho-Chul
authors.initials: HC
authors.lastname: Shin
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Jae Han
authors.initials: JH
authors.lastname: Shim
authors.affiliation: Department of Chemistry, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis* / Q000494:pharmacology; D031804:Rumex / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D059808:Polyphenols
keywords: Antioxidant activity; Flavonoids; Polyphenols; Rumex nervosus; Tandem mass spectrometry