title: Effect of hyperprolactinemia on PRL-receptor expression and activation of Stat and Mapk cell signaling in the prostate of long-term sexually-active rats.
abstract: The abnormal elevation of serum PRL, referred to as hyperprolactinemia (HyperPRL), produces alterations in several reproductive parameters of male rats such as penile erection or decreased tendency to reach ejaculation. Additionally, this situation produces a significant modification of prostate histology, as observed in the epithelial structure and alveolar area, which could reach a level of hyperplasia in the long-term. In this tissue, HyperPRL produces an increase in expression of PRL receptors and activation of the Stat3 signaling pathway that is correlated with the evolution of prostate pathologies. However, the impact of HyperPRL in long-term sexually active male rats is unknown. In this work, using constantly copulating Wistar male rats with induced HyperPRL, we analyzed the level of serum PRL, the effect on prostate PRL receptors, and activation of pStat3, pStat5 and Mapk signaling pathways. Two procedures to induce HyperPRL were employed, comprising daily IP administration or adenohypophysis transplant, and although neither affected the execution of sexual behavior, the serum PRL profile following successive ejaculations was affected. Messenger RNA expression of the short and long isoforms of the PRL receptor at the ventral prostate was affected in different ways depending on the procedure to induce HyperPRL. The ventral prostate did not show any modification in terms of activation of the pStat5 signaling pathway in subjects with daily administration of PRL, although this was significantly increased in ADH transplanted subjects in the second and fourth consecutive ejaculation. A similar profile was found for the pStat3 pathway which additionally showed a significant increase in the third and fourth ejaculation of daily-injected subjects. The Mapk signaling pathway did not show any modifications in subjects with daily administration of PRL, but showed a significant increase in the second and third ejaculations of subjects with ADH transplants. Thus, although sexual behavior was not modified, HyperPRL modified the expression of PRL receptors and the activation of signal pathways in the prostate tissue. Hence, it is probable that prostatic alterations precede the sexual behavioral deficits observed in subjects with HyperPRL.
authors.affiliation: Facultad de QFB, Universidad Veracruzana, Xalapa, Ver., Mexico.
authors.forename: Luz I
authors.initials: LI
authors.lastname: Pascual-Mathey
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Ver., Mexico.
authors.forename: Fausto
authors.initials: F
authors.lastname: Rojas-Duran
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Ver., Mexico.
authors.forename: Gonzalo E
authors.initials: GE
authors.lastname: Aranda-Abreu
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Ver., Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Manzo
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Ver., Mexico.
authors.forename: Deissy
authors.initials: D
authors.lastname: Herrera-Covarrubias
authors.affiliation: Doctorado en Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Ver., Mexico.
authors.forename: David A
authors.initials: DA
authors.lastname: Muñoz-Zavaleta
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Ver., Mexico.
authors.forename: Luis I
authors.initials: LI
authors.lastname: Garcia
authors.affiliation: Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Ver., Mexico. Electronic address: elenahernandez@uv.mx.
authors.forename: Ma Elena
authors.initials: ME
authors.lastname: Hernandez
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003307:Copulation / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006966:Hyperprolactinemia / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D010052:Ovariectomy; D011388:Prolactin / Q000009:adverse effects / Q000097:blood; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011981:Receptors, Prolactin / Q000378:metabolism*; D050791:STAT Transcription Factors / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013256:Steroids / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011981:Receptors, Prolactin; D050791:STAT Transcription Factors; D013256:Steroids; D011388:Prolactin; D020929:Mitogen-Activated Protein Kinase Kinases
keywords: Prostate pathology; Serum prolactin; Sexual behavior; Signaling pathways