title: Oxidative stress in the red blood cells of patients with primary open-angle glaucoma.
abstract: BACKGROUND
  This study was designed to examine oxidative and antioxidative changes in the red blood cells (RBCs) of patients presenting with glaucomatous degeneration.
  
  
  METHODS
  The experimental design was a case-control study of strictly selected patients who required antiglaucomatous surgery during primary open-angle glaucoma despite relatively regulated intraocular pressure (IOP) (POAG group, n = 30) and patients who underwent an operation for nonpathological cataracts (cataract group, n = 25). The activities of total superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT), as well as the concentration of malondialdehyde (MDA), were measured. Glaucomatous damage was estimated from a transient pattern electroretinogram.
  
  
  RESULTS
  Significant increases in GPX (p = 0.026) and CAT (p = 0.000) activity were noted in the RBCs of POAG patients compared to those of cataract patients. Although SOD was elevated in patients with POAG, the differences compared to cataract controls were not significant (p = 0.079). MDA concentrations were significantly increased in the glaucoma group compared to the cataract controls.
  
  
  CONCLUSIONS
  An oxidative disorder primarily represented by catalase upregulation was observed during the course of glaucoma.
authors.affiliation: Department and Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rokicki
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia, Katowice, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Zalejska-Fiolka
authors.affiliation: Department and Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Pojda-Wilczek
authors.affiliation: Department and Clinic of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Kabiesz
authors.affiliation: Department of Radiotherapy, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Majewski
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000276:immunology*
keywords: CAT; GPX; Glaucoma; MDA; SOD; erythrocyte; oxidative stress