title: Interventional effects of da-cheng-qi decoction on enteric nerve system in a rat model of multiple organ dysfunction syndrome.
abstract: In this study, we investigate the morphologic changes of enteric nerve system (ENS) and the expression of neurotransmitters, acetylcholine (ACh), substance P (SP), vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS), in small bowel of rats undergoing multiple organ dysfunction syndrome (MODS). Undergoing MODS, fluorescence integral optical density (IOD) value of enteric nerve fibers were significantly decreased (P<0.05), and the network structure of ENS was destroyed. The expression of ACh, SP, VIP and NOS was inhibited, IOD value of the four neurotransmitters was significantly decreased (P<0.05). After intervention of DCQD, the fluorescence IOD value of enteric nerves were significantly increased (P<0.05), and the network structure of ENS was repaired. The expression of ACh, SP, VIP and NOS was recovered, fluorescence IOD value of the four neurotransmitters was significantly increased (P<0.05). In conclusion, the gastrointestinal motility disorders undergoing MODS may be closely related to the morphology destroy of ENS and down regulation of neurotransmitters (ACh, SP, VIP and NOS) expression. DCQD could promote gastrointestinal motility through protecting the morphology of ENS and up regulation of neurotransmitters (ACh, SP, VIP and NOS) expression.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Dalian Medical University Dalian 116011, Liaoning Province, China.
authors.forename: Ming-Zheng
authors.initials: MZ
authors.lastname: Xie
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Dalian Medical University Dalian 116011, Liaoning Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Luo
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Dalian Medical University Dalian 116011, Liaoning Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ma
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Dalian Medical University Dalian 116011, Liaoning Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Dalian Medical University Dalian 116011, Liaoning Province, China.
authors.forename: De-Hua
authors.initials: DH
authors.lastname: Wang
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Dalian Medical University Dalian 116011, Liaoning Province, China.
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Qi
keywords: Multiple organ dysfunction syndrome; da-cheng-qi decoction; enteric nerve system; gastrointestinal motility