title: Novel tubular constructs for urinary diversion: a biocompatibility study in pigs.
abstract: The use of bowel tissue for urinary diversion can be associated with severe complications, and regenerative medicine may circumvent this by providing an engineered conduit. In this study, a novel tubular construct was identified for this purpose. Three constructs (diameter 15 mm) were prepared from type I collagen and either (a) a semi-biodegradable Vypro II polymer (COL-Vypro), (b) a rapidly biodegradable Vicryl polymer (COL-Vicryl) or (c) an additional collagenous layer (COL-DUAL). After freezing, lyophilization and crosslinking, all constructs showed a porous structure with a two-fold higher strength for the polymer-containing constructs. These constructs were connected to full bladder defects of 11 female pigs and evaluated after 1 (n = 4) or 3 months (n = 5). With respect to surgical handling, the polymer-containing constructs were superior. All pigs voided normally without leakage and the survival rate was 82%. For the implanted COL-Vypro constructs (8/9), stone formation was observed. COL-DUAL and COL-Vicryl showed better biocompatibility and only small remnants were found 1 month post-implantation. Histological and immunohistochemical analysis showed the best regeneration for COL-Vicryl with respect to urothelium; muscle pedicles and elastin formation were best developed in the COL-Vicryl constructs. In this study, COL-Vicryl constructs were superior in both biocompatibility and bladder tissue regeneration and have high potential for artificial urinary diversions. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Urology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Marije
authors.initials: M
authors.lastname: Sloff
authors.affiliation: Department of Urology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Vasilis
authors.initials: V
authors.lastname: Simaioforidis
authors.affiliation: Department of Urology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Geutjes
authors.affiliation: Department of Biochemistry, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Henk R
authors.initials: HR
authors.lastname: Hoogenkamp
authors.affiliation: Department of Biochemistry, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Toin H
authors.initials: TH
authors.lastname: van Kuppevelt
authors.affiliation: Department of Biochemistry, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Willeke F
authors.initials: WF
authors.lastname: Daamen
authors.affiliation: Department of Urology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Egbert
authors.initials: E
authors.lastname: Oosterwijk
authors.affiliation: Department of Urology, Radboud University Medical Centre, Nijmegen, The Netherlands.|Radboud University Medical Centre, Amalia Children's Hospital, Nijmegen, The Netherlands.
authors.forename: Wout F
authors.initials: WF
authors.lastname: Feitz
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D024042:Collagen Type I / Q000737:chemistry; D005260:Female; D008422:Materials Testing; D011098:Polyglactin 910 / Q000737:chemistry; D011126:Polypropylenes / Q000737:chemistry; D013552:Swine; D001743:Urinary Bladder / Q000503:physiopathology / Q000601:surgery; D014547:Urinary Diversion / Q000379:methods*
chemical_list: D024042:Collagen Type I; D011126:Polypropylenes; C510058:VyproII; D011098:Polyglactin 910
keywords: bladder biocompatibility; collagen; polymer mesh; porcine model; tubular constructs; urinary diversion