title: Protective efficacy of Streptococcus iniae derived enolase against Streptococcal infection in a zebrafish model.
abstract: Enolase (ENO) is one of the surface-exposed proteins of Streptococcus iniae, which previously had been identified as a plasminogen-binding protein. In this study, ENO was evaluated to induce cross-protective immunity against S. iniae and Streptococcus parauberis which are major pathogens causing streptococcosis in fish. Immunoblot analysis shows that S. iniae recombinant ENO (rENO) produced in Escherichia coli was cross-reactive with antisera against S. iniae, and S. parauberis serotype I and II. In the challenge experiment of streptococcal infection after vaccination in zebrafish, rENO elicited a similar protection with a whole cell bacterin against S. iniae and S. parauberis, which suggests its feasibility as an efficient vaccine against streptococcosis.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea; Choong Ang Vaccine Laboratories Co., Ltd., Daejeon 305-348, Republic of Korea.
authors.forename: Juver D
authors.initials: JD
authors.lastname: Membrebe
authors.affiliation: Biotechnology Process Engineering Center, KRIBB, Cheongju 363-883, Republic of Korea.
authors.forename: Nam-Kyung
authors.initials: NK
authors.lastname: Yoon
authors.affiliation: Biotechnology Process Engineering Center, KRIBB, Cheongju 363-883, Republic of Korea.
authors.forename: Minhee
authors.initials: M
authors.lastname: Hong
authors.affiliation: Bionanotechnology Research Center, KRIBB, Cheongju 363-883, Republic of Korea; Major of Functional Genomics, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea.
authors.forename: Jeongsoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Biotechnology Process Engineering Center, KRIBB, Cheongju 363-883, Republic of Korea; Department of Biotechnology Process Engineering, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea.
authors.forename: Hongweon
authors.initials: H
authors.lastname: Lee
authors.affiliation: Biological and Chemical engineering, Hongik University, Sejong 339-701, Republic of Korea.
authors.forename: Kyoungmoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Choong Ang Vaccine Laboratories Co., Ltd., Daejeon 305-348, Republic of Korea.
authors.forename: Sin-hye
authors.initials: SH
authors.lastname: Seo
authors.affiliation: Choong Ang Vaccine Laboratories Co., Ltd., Daejeon 305-348, Republic of Korea.
authors.forename: Injoong
authors.initials: I
authors.lastname: Yoon
authors.affiliation: Choong Ang Vaccine Laboratories Co., Ltd., Daejeon 305-348, Republic of Korea.
authors.forename: Sungsik
authors.initials: S
authors.lastname: Yoo
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea. Electronic address: dohnanyi@kaist.ac.kr.
authors.forename: Yeu-Chun
authors.initials: YC
authors.lastname: Kim
authors.affiliation: Biotechnology Process Engineering Center, KRIBB, Cheongju 363-883, Republic of Korea; Department of Biotechnology Process Engineering, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea. Electronic address: ahnjo@kribb.re.kr.
authors.forename: Jungoh
authors.initials: J
authors.lastname: Ahn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D004195:Disease Models, Animal; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D010751:Phosphopyruvate Hydratase / Q000276:immunology*; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D015027:Zebrafish
chemical_list: D001426:Bacterial Proteins; D022541:Streptococcal Vaccines; D014614:Vaccines, Synthetic; D010751:Phosphopyruvate Hydratase
keywords: Streptococcus iniae; Streptococcus parauberis; Subunit vaccine; Zebrafish; α-Enolase