title: Surface modification of a polyethylene film for anticoagulant and anti-microbial catheter.
abstract: A functional anticoagulant and anti-bacterial coating for polyethylene (PE) films is described. The stepwise preparation of this nanocomposite surface coating involves O2plasma etching of PE film, carbodiimide coupling of cysteamine to the etched PE film, binding of Ag to sulfhydryl groups of cysteamine, and assembly of heparin capped AgNPs on the PE film. The nanocomposite film and its components were characterized by1H-nuclear magnetic resonance spectroscopy, attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and field emission-scanning electron microscopy. The resulting PE films demonstrate anticoagulant activity using a hemoglobin whole blood clotting assay, and anti-bacterial activity againstBacillus cereus3551 (Gram-positive) andEscherichia coliBL21 (Gram-negative) bacteria. The hydrophilicity of the heparin coated PE was determined by contact angle measurements; and the stability of the nanocomposite film, with respect to Ag leaching, was assessed by atomic absorption spectroscopy.
authors.affiliation: Department of Physics and Key Laboratory of ATMMT Ministry of Education, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Miao
authors.affiliation: Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA.
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Cai
authors.affiliation: Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Koh
authors.affiliation: Center for Future Energy Systems, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA.
authors.forename: Trevor J
authors.initials: TJ
authors.lastname: Simmons
authors.affiliation: The Pharmaceutical Research Institute, Albany College of Pharmacy, Albany, New York, 12208, USA.
authors.forename: Shaker A
authors.initials: SA
authors.lastname: Mousa
authors.affiliation: Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
keywords: anti-bacterial; anticoagulant; catheter; nanocomposite; polyethylene coating