title: Cytokine production and mRNA expression in pulmonary tuberculosis patients and their household contacts of younger age group (15-25years).
abstract: Household contacts of tuberculosis patients are at high risk of infection and development of active disease. In this study we evaluated the cytokine production and mRNA expression of IFN-γ, TNF-α, IL-10&IL-6 stimulated with r32kDa M. bovis BCGAg in active pulmonary tuberculosis patients (APTB), household contacts (HHC) and healthy controls (HC). The results showed the stimulated levels of IFN-γ and TNF-α were low while IL-10 levels were high in APTB and HHC compared to HC. IL-6 has not shown any significant difference. The mRNA expression of TNF- α was 8 fold high in HCs compared to APTB and HHC. The IL-6 expression was 2.2 fold &1 fold less in APTB and HHC compared to HCs. Multinomial logistic regression analysis indicated that the stimulated levels of IFN-γ & IL-6 and sex significantly predicted the HHC group from HCs at p<0.05.In conclusion further follow up studies with r32kd antigen might help to identify the high risk individuals.
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, India. Electronic address: lavanya.ou@gmail.com.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Joshi
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, India. Electronic address: mnaidu87@gmail.com.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Ponnana
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, India. Electronic address: stramya1115@gmail.com.
authors.forename: Ramya
authors.initials: R
authors.lastname: Sivangala
authors.affiliation: Department of Transplant Biology& Stem Cell, Global Hospital Hyderabad, Telangana, India. Electronic address: lkiran@globalhospitalsindia.com.
authors.forename: Lakshmi Kiran
authors.initials: LK
authors.lastname: Chelluri
authors.affiliation: Department of Genetics, Osmania University, Hyderabad, Telangana, India. Electronic address: prathinallari@yahoo.com.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Nallari
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, India; LEPRA India, Blue Peter Public Health & Research Centre, Cherlapally, Hyderabad, Telangana, India. Electronic address: vijayavalluri@gmail.com.
authors.forename: Vijaya Lakshmi
authors.initials: VL
authors.lastname: Valluri
authors.affiliation: Bhagwan Mahavir Medical Research Centre, Hyderabad, Telangana, India; Department of Genetics, Osmania University, Hyderabad, Telangana, India. Electronic address: sumanlathag@yahoo.com.
authors.forename: Sumanlatha
authors.initials: S
authors.lastname: Gaddam
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000942:Antigens, Bacterial / Q000276:immunology; D016022:Case-Control Studies; D049109:Cell Proliferation; D002478:Cells, Cultured; D016358:Contact Tracing; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054884:Host-Pathogen Interactions; D006798:Housing; D006801:Humans; D059425:Interferon-gamma Release Tests; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D016015:Logistic Models; D008297:Male; D015999:Multivariate Analysis; D009163:Mycobacterium bovis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000472:pathogenicity; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012307:Risk Factors; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000382:microbiology / Q000635:transmission*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines; D012333:RNA, Messenger
keywords: Cytokines; Household contacts; Mycobacterium tuberculosis; r32kd M. bovis BCG