title: SSRs transferability and genetic diversity of three allogamous ryegrass species.
abstract: Simple sequence repeat (SSR) markers are widely applied in studies of plant molecular genetics due to their abundance in the genome, codominant nature, and high repeatability. However, microsatellites are not always available for the species to be studied and their isolation could be time- and cost-consuming. To investigate transferability in cross-species applications, 102 primer pairs previously developed in ryegrass and tall fescue were amplified across three allogamous ryegrass species including Lolium rigidum, Lolium perenne and Lolium multiflorum. Their highly transferability (100%) were evidenced. While, most of these markers were multiple loci, only 17 loci were selected for a robust, single-locus pattern, which may be due to the recentness of the genome duplication or duplicated genomic regions, as well as speciation. A total of 87 alleles were generated with an average of 5.1 per locus. The mean polymorphism information content (PIC) and observed heterozygosity (Ho) values at genus was 0.5532 and 0.5423, respectively. Besides, analysis of molecular variance (AMOVA) revealed that all three levels contributed significantly to the overall genetic variation, with the species level contributing the least (P<0.001). Also, the unweighted pair group method with arithmetic averaging dendrogram (UPGMA), Bayesian model-based STRUCTURE analysis and the principal coordinate analysis (PCoA) showed that accessions within species always tended to the same cluster firstly and then to related species. The results showed that these markers developed in related species are transferable efficiently across species, and likely to be useful in analyzing genetic diversity.
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Guo
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Kai-Xin
authors.initials: KX
authors.lastname: Fu
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Xin-Quan
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Lin-Kai
authors.initials: LK
authors.lastname: Huang
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Yan
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, PR China. Electronic address: maroar@126.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
mesh_terms: D000483:Alleles; D001499:Bayes Theorem; D001947:Breeding / Q000379:methods; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D008129:Lolium / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics; D013045:Species Specificity
chemical_list: D005819:Genetic Markers
keywords: Genetic diversity; Lolium; Simple sequence repeats (SSR); Transferability