title: Light-up fluorophore--DNA aptamer pair for label-free turn-on aptamer sensors.
abstract: We developed a light-up fluorophore-DNA aptamer pair for label-free aptamer sensors that fluoresce upon binding to the analyte. A 42mer DNA aptamer binding to the environment-sensitive fluorophore, dapoxyl, which increased the fluorescence by more than 700-fold upon binding, was successfully used to construct aptamer sensors by fusion with analyte-binding DNA aptamers.
authors.affiliation: Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@stf.teu.ac.jp.
authors.forename: Teru
authors.initials: T
authors.lastname: Kato
authors.affiliation: Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@stf.teu.ac.jp.
authors.forename: Ippei
authors.initials: I
authors.lastname: Shimada
authors.affiliation: Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@stf.teu.ac.jp.
authors.forename: Ryota
authors.initials: R
authors.lastname: Kimura
authors.affiliation: Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan. kato@stf.teu.ac.jp.
authors.forename: Masumi
authors.initials: M
authors.lastname: Hyuga
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D008027:Light; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D004247:DNA