title: Single-Particle Tracking Shows that a Point Mutation in the Carnivore Parvovirus Capsid Switches Binding between Host-Specific Transferrin Receptors.
abstract: Determining how viruses infect new hosts via receptor-binding mechanisms is important for understanding virus emergence. We studied the binding kinetics of canine parvovirus (CPV) variants isolated from raccoons-a newly recognized CPV host-to different carnivore transferrin receptors (TfRs) using single-particle tracking. Our data suggest that CPV may utilize adhesion-strengthening mechanisms during TfR binding and that a single mutation in the viral capsid at VP2 position 300 can profoundly alter receptor binding and infectivity.
authors.affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, USA.
authors.forename: Donald W
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Baker Institute for Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Allison
authors.affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, USA.
authors.forename: Kaitlyn B
authors.initials: KB
authors.lastname: Bacon
authors.affiliation: Baker Institute for Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA crp3@cornell.edu sd386@cornell.edu.
authors.forename: Colin R
authors.identifier: http://orcid.org/0000-0002-1836-6655
authors.initials: CR
authors.lastname: Parrish
authors.affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, USA crp3@cornell.edu sd386@cornell.edu.
authors.forename: Susan
authors.initials: S
authors.lastname: Daniel
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D004285:Dogs; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods; D017993:Parvovirus, Canine / Q000502:physiology*; D017354:Point Mutation; D011485:Protein Binding; D011821:Raccoons; D011990:Receptors, Transferrin / Q000378:metabolism*; D013194:Staining and Labeling; D056189:Viral Tropism
chemical_list: D036022:Capsid Proteins; D011990:Receptors, Transferrin