title: The successful induction of T-cell and antibody responses by a recombinant measles virus-vectored tetravalent dengue vaccine provides partial protection against dengue-2 infection.
abstract: Dengue has a major impact on global public health, and the use of dengue vaccine is very limited. In this study, we evaluated the immunogenicity and protective efficacy of a dengue vaccine made from a recombinant measles virus (MV) that expresses envelope protein domain III (ED3) of dengue-1 to 4. Following immunization with the MV-vectored dengue vaccine, mice developed specific interferon-gamma and antibody responses against dengue virus and MV. Neutralizing antibodies against MV and dengue viruses were also induced, and protective levels of FRNT50 ≥ 10 to 4 serotypes of dengue viruses were detected in the MV-vectored dengue vaccine-immunized mice. In addition, specific interferon-gamma and antibody responses to dengue viruses were still induced by the MV-vectored dengue vaccine in mice that were pre-infected with MV. This finding suggests that the pre-existing immunity to MV did not block the initiation of immune responses. By contrast, mice that were pre-infected with dengue-3 exhibited no effect in terms of their antibody responses to MV and dengue viruses, but a dominant dengue-3-specific T-cell response was observed. After injection with dengue-2, a detectable but significantly lower viremia and a higher titer of anti-dengue-2 neutralizing antibodies were observed in MV-vectored dengue vaccine-immunized mice versus the vector control, suggesting that an anamnestic antibody response that provided partial protection against dengue-2 was elicited. Our results with regard to T-cell responses and the effect of pre-immunity to MV or dengue viruses provide clues for the future applications of an MV-vectored dengue vaccine.
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.
authors.forename: Hui-Mei
authors.initials: HM
authors.lastname: Hu
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.|b Graduate Institute of Immunology, China Medical University , Taichung , Taiwan.
authors.forename: Hsin-Wei
authors.initials: HW
authors.lastname: Chen
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Hsiao
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.
authors.forename: Szu-Hsien
authors.initials: SH
authors.lastname: Wu
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.
authors.forename: Han-Hsuan
authors.initials: HH
authors.lastname: Chung
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.
authors.forename: Chun-Hsiang
authors.initials: CH
authors.lastname: Hsieh
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.|b Graduate Institute of Immunology, China Medical University , Taichung , Taiwan.
authors.forename: Pele
authors.initials: P
authors.lastname: Chong
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.|b Graduate Institute of Immunology, China Medical University , Taichung , Taiwan.
authors.forename: Chih-Hsiang
authors.initials: CH
authors.lastname: Leng
authors.affiliation: a National Institute of Infectious Disease and Vaccinology, National Health Research Institutes , Zhunan Town , Taiwan.|b Graduate Institute of Immunology, China Medical University , Taichung , Taiwan.
authors.forename: Chien-Hsiung
authors.initials: CH
authors.lastname: Pan
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D053059:Dengue Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004337:Drug Carriers; D007371:Interferon-gamma / Q000378:metabolism; D008459:Measles virus / Q000235:genetics* / Q000276:immunology; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D053059:Dengue Vaccines; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D007371:Interferon-gamma