title: A novel gene delivery composite system based on biodegradable folate-poly (ester amine) polymer and thermosensitive hydrogel for sustained gene release.
abstract: Local anti-oncogene delivery providing high local concentration of gene, increasing antitumor effect and decreasing systemic side effects is currently attracting interest in cancer therapy. In this paper, a novel local sustained anti-oncogene delivery system, PECE thermoresponsive hydrogel containing folate-poly (ester amine) (FA-PEA) polymer/DNA (tumor suppressor) complexes, is demonstrated. First, a tumor-targeted biodegradable folate-poly (ester amine) (FA-PEA) polymer based on low-molecular-weight polyethyleneimine (PEI) was synthesized and characterized, and the application for targeted gene delivery was investigated. The polymer had slight cytotoxicity and high transfection efficiency in vitro compared with PEI 25k, which indicated that FA-PEA was a potential vector for targeted gene delivery. Meanwhile, we successfully prepared a thermoresponsive PECE hydrogel composite containing FA-PEA/DNA complexes which could contain the genes and slowly release the genes into cells. We concluded the folate-poly (ester amine) (FA-PEA) polymer would be useful for targeted gene delivery, and the novel gene delivery composite based on biodegradable folate-poly (ester amine) polymer and thermosensitive PECE hydrogel showed potential for sustained gene release.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P.R. China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P.R. China.
authors.forename: YanPeng
authors.initials: Y
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P.R. China.
authors.forename: QingFa
authors.initials: Q
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qu
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, 22 Shuang Yong Rd. Nanning, Guangxi 530021, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Su
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, 22 Shuang Yong Rd. Nanning, Guangxi 530021, China.
authors.forename: XiaoLing
authors.initials: X
authors.lastname: Lu
authors.affiliation: National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, 22 Shuang Yong Rd. Nanning, Guangxi 530021, China.
authors.forename: YongXiang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P.R. China.
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D017209:Apoptosis; D001672:Biocompatible Materials; D002460:Cell Line; D005492:Folic Acid / Q000737:chemistry; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D066244:Proton Magnetic Resonance Spectroscopy; D014162:Transfection; D019076:Transgenes
chemical_list: D001672:Biocompatible Materials; D011073:Polyamines; D011091:Polyesters; D011108:Polymers; C516446:poly(caprolactone-block-ethyleneimine); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005492:Folic Acid