title: The Expression of miR-192 and Its Significance in Diabetic Nephropathy Patients with Different Urine Albumin Creatinine Ratio.
abstract: OBJECTIVE
  To investigate the expression of miR-192 and its significance in diabetic nephropathy (DN) patients.
  
  
  METHODS
  464 patients with type 2 diabetes mellitus (T2DM) were divided into normal albuminuria group (NA, n = 157), microalbuminuria group (MA, n = 159), and large amount of albuminuria group (LA, n = 148). 127 healthy persons were selected as the control group (NC, n = 127). The serum miR-192 levels were detected by Real-Time PCR and transforming growth factor-β1 (TGF-β1) and fibronectin (FN) were detected by enzyme-linked immunosorbent assay. The relationships among these parameters were analyzed by Pearson correlation analysis and multiple linear regression analysis.
  
  
  RESULTS
  The miR-192 in the LA group was significantly lower than other groups, which was lower in the MA group than in the NA group (P < 0.01). The TGF-β1 and FN in the LA group were significantly higher than other groups, which were higher in the MA group than in the NA group (P < 0.01). The expression of miR-192 was negatively correlated with TGF-β1, FN, and Ln (UACR) and miR-192, TGF-β1, and FN were independent relevant factors affecting Ln (UACR) in T2DM (P < 0.01).
  
  
  CONCLUSIONS
  These findings indicate that the levels of miR-192 were lower accompanied by the decrease of urine albumin creatinine ratio (UACR) and the association between miR-192 and nephritic fibrosis in DN.
authors.affiliation: Geriatrics Department, The First Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Ma
authors.affiliation: Endocrine Department, The First Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Canlu
authors.initials: C
authors.lastname: Lu
authors.affiliation: Endocrine Department, The People Hospital of Liaoning Province, Shenyang, Liaoning, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Lv
authors.affiliation: Endocrine Department, The First Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Can
authors.initials: C
authors.lastname: Wu
authors.affiliation: Endocrine Department, The First Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000368:Aged; D000419:Albuminuria / Q000378:metabolism*; D003404:Creatinine / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000652:urine; D003928:Diabetic Nephropathies / Q000378:metabolism* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D005355:Fibrosis / Q000473:pathology; D005786:Gene Expression Regulation; D005951:Glucose Tolerance Test; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009393:Nephritis / Q000652:urine; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: C067625:FN1 protein, human; D005353:Fibronectins; C546783:MIRN192 microRNA, human; D035683:MicroRNAs; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D003404:Creatinine