title: Minocycline induces protective autophagy in vascular endothelial cells exposed to anin vitromodel of ischemia/reperfusion-induced injury.
abstract: Minocycline has been reported to exhibit advantageous effects on ischemic stroke; however, the precise mechanism of minocycline remains to be established. In the present study, human umbilical vein endothelial cells (HUVECs) were subjected toin vitrosimulated ischemia/reperfusion conditions to determine the potential effect of minocycline-induced autophagy on HUVEC damage under oxygen-glucose deprivation/reperfusion (OGD/R). The study demonstrated that minocycline enhanced autophagy in a dose-dependent manner in HUVECs exposed to OGD/R, and only low-dose minocycline protected HUVECs from OGD/R-induced damage. Subsequently, 3-methyladenine (3-MA) was added into the culture media and the protective effect of minocycline was abolished. At the same time, it has been observed that simultaneous treatment with 3-MA also inhibited the autophagy activity induced by minocycline. This finding could suggest that autophagy induced by minocycline serves as one of the potential protective mechanism underlying the beneficial effects of minocycline on ischemic injury.
authors.affiliation: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, P.R. China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Dong
authors.affiliation: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, P.R. China.
authors.forename: Shigeng
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Cheng
authors.affiliation: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, P.R. China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Ye
authors.affiliation: Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310013, P.R. China.
authors.forename: Gaoli
authors.initials: G
authors.lastname: Zheng
keywords: autophagy; human umbilical vein endothelial cells; ischemia/reperfusion; minocycline