title: Proteome quantification of cotton xylem sap suggests the mechanisms of potassium-deficiency-induced changes in plant resistance to environmental stresses.
abstract: Proteomics was employed to investigate the molecular mechanisms of apoplastic response to potassium(K)-deficiency in cotton. Low K (LK) treatment significantly decreased the K and protein contents of xylem sap. Totally, 258 peptides were qualitatively identified in the xylem sap of cotton seedlings, of which, 90.31% were secreted proteins. Compared to the normal K (NK), LK significantly decreased the expression of most environmental-stress-related proteins and resulted in a lack of protein isoforms in the characterized proteins. For example, the contents of 21 Class Ш peroxidase isoforms under the LK were 6 to 44% of those under the NK and 11 its isoforms were lacking under the LK treatment; the contents of 3 chitinase isoforms under LK were 11-27% of those under the NK and 2 its isoforms were absent under LK. In addition, stress signaling and recognizing proteins were significantly down-regulated or disappeared under the LK. In contrast, the LK resulted in at least 2-fold increases of only one peroxidase, one protease inhibitor, one non-specific lipid-transfer protein and histone H4 and in the appearance of H2A. Therefore, K deficiency decreased plant tolerance to environmental stresses, probably due to the significant and pronounced decrease or disappearance of a myriad of stress-related proteins.
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Maoni
authors.initials: M
authors.lastname: Chao
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Bu
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Juxiang
authors.initials: J
authors.lastname: Tang
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, China.|Department of Biology, East Carolina University, Greenville, NC 27858, US.
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
mesh_terms: D005075:Biological Evolution; D016000:Cluster Analysis; D004777:Environment; D020869:Gene Expression Profiling; D003368:Gossypium / Q000145:classification / Q000502:physiology*; D008903:Minerals / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D011191:Potassium Deficiency / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D019655:Quantitative Trait, Heritable; D036226:Seedlings; D013312:Stress, Physiological; D052584:Xylem / Q000378:metabolism*
chemical_list: D008903:Minerals; D010940:Plant Proteins; D020543:Proteome; D011188:Potassium