title: Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARα) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells.
abstract: OBJECTIVE
  To search for a possible role of Peroxisome Proliferator-Activated Receptor α (PPARα), a molecular partner of the Aryl hydrocarbon receptor Interacting Protein (AIP), in somatotropinomas.
  
  
  METHODS
  Tumours from 51 acromegalic patients were characterized for PPARα and AIP expression by immunohistochemistry (IHC) and/or Real Time RT-PCR. Data were analysed according to tumour characteristics and pre-operative treatment with somatostatin analogues (SSA). The effects of fenofibrate were studied in GH3 cells in vitro.
  
  
  RESULTS
  PPARα was expressed in most somatotropinomas. A modest relationship was found between PPARα and AIP expression, both being significantly higher in the presence of pre-operative SSA. However, only AIP expression was influenced by the response to treatment. Dual effects of fenofibrate were observed in GH3 cells, consisting of cell growth inhibition and an increase in GH secretion inhibited by octreotide.
  
  
  CONCLUSIONS
  PPARα is a new player in somatotropinomas. Potential interactions between PPARα agonists and SSA may deserve further investigation.
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, AQ, Italy; Neuromed Institute, IRCCS, Pozzilli, IS, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rotondi
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, AQ, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Modarelli
authors.affiliation: Neuromed Institute, IRCCS, Pozzilli, IS, Italy.
authors.forename: Maria-Antonietta
authors.initials: MA
authors.lastname: Oliva
authors.affiliation: Endocrinology, CHU of Liège, University of Liège, Belgium.
authors.forename: Liliya
authors.initials: L
authors.lastname: Rostomyan
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, AQ, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Sanita
authors.affiliation: Division of Pathology, "San Salvatore" Hospital, L'Aquila, AQ, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Ventura
authors.affiliation: Endocrinology, CHU of Liège, University of Liège, Belgium.
authors.forename: Adrian F
authors.initials: AF
authors.lastname: Daly
authors.affiliation: Neuromed Institute, IRCCS, Pozzilli, IS, Italy; Neurosurgery, Department of Neurology and Psychiatry, University "La Sapienza", Rome, RM, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Esposito
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, AQ, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Angelucci
authors.affiliation: Neuromed Institute, IRCCS, Pozzilli, IS, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Arcella
authors.affiliation: Neuromed Institute, IRCCS, Pozzilli, IS, Italy; Neuropathology, Department of Radiological, Oncological and Anatomopathological Sciences, University "La Sapienza", Rome, RM, Italy.
authors.forename: Felice
authors.initials: F
authors.lastname: Giangaspero
authors.affiliation: Endocrinology, CHU of Liège, University of Liège, Belgium.
authors.forename: Albert
authors.initials: A
authors.lastname: Beckers
authors.affiliation: Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, AQ, Italy; Neuromed Institute, IRCCS, Pozzilli, IS, Italy. Electronic address: marielise.jaffrain@univaq.it.
authors.forename: Marie-Lise
authors.initials: ML
authors.lastname: Jaffrain-Rea
mesh_terms: D000236:Adenoma / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D049109:Cell Proliferation; D002648:Child; D005260:Female; D011345:Fenofibrate / Q000494:pharmacology*; D049912:Growth Hormone-Secreting Pituitary Adenoma / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D047493:PPAR alpha / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism / Q000473:pathology; D051381:Rats; D055815:Young Adult
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D047493:PPAR alpha; C103265:aryl hydrocarbon receptor-interacting protein; D011345:Fenofibrate
keywords: Acromegaly; Aryl hydrocarbon receptor interacting protein (AIP); Fenofibrate; GH(3) cells; Peroxisome proliferator-activated receptor (PPARα); Somatostatin analogues