title: Embryonic transcriptome of the brackishwater amphipod Gammarus chevreuxi.
abstract: Environmental change can dramatically alter the development of aquatic organisms. While the effect of such change on physiological and morphological ontogenies is becoming clearer, the molecular mechanisms underpinning them are largely unexplored. Characterizing these mechanisms is often limited by the lack of molecular resources. We have applied Illumina HiSeq sequencing to RNA isolated from different developmental stages of the brackishwater amphipod Gammarus chevreuxi. Over 52.6M paired-end reads were assembled de novo into 172,081 contigs, representing 118,812 potential genes. The assembly generated constitutes a reference embryonic transcriptome for an ecologically-important aquatic shredder species. This resource will contribute to our understanding of the mechanisms underpinning the development of physiological function through functional, comparative and quantitative expression studies. It will also allow the identification of candidate biomarkers for assessing the impact of environmental stressors in estuarine systems.
authors.affiliation: Marine Biology and Ecology Research Centre, School of Marine Science and Engineering, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: manuela.truebanogarcia@plymouth.ac.uk.
authors.forename: Manuela
authors.initials: M
authors.lastname: Truebano
authors.affiliation: Marine Biology and Ecology Research Centre, School of Marine Science and Engineering, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: oliver.tills@plymouth.ac.uk.
authors.forename: Oliver
authors.initials: O
authors.lastname: Tills
authors.affiliation: Marine Biology and Ecology Research Centre, School of Marine Science and Engineering, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK. Electronic address: j.i.spicer@plymouth.ac.uk.
authors.forename: John I
authors.initials: JI
authors.lastname: Spicer
mesh_terms: D033304:Amphipoda / Q000196:embryology / Q000235:genetics*; D000818:Animals; D004739:England; D000068357:Saline Waters; D017423:Sequence Analysis, RNA; D059467:Transcriptome
keywords: Amphipod; Development; Embryonic transcriptome; Gammarus