title: Whole genome characterization of a chelonian orthoreovirus strain identifies significant genetic diversity and may classify reptile orthoreoviruses into distinct species.
abstract: In this study we report the sequence and phylogenetic characterization of an orthoreovirus strain, CH1197/96, isolated from a spur-thighed tortoise (Testudo graeca) on chicken embryo fibroblast cells. The 23,957 bp long genome sequence was obtained by combined use of semiconductor and capillary sequencing. Although the genomic characterization showed that the virus was most similar to the bush viper reovirus strain, 47/02, and in phylogenies performed with all segments the two strains formed a monophyletic group, the nucleotide (48.4-70.3%) and amino acid (39.2-80.7%) sequence identity values were moderate between the two reptile origin reoviruses. Based on our results and existing classification criteria for the genus Orthoreovirus, the tortoise reovirus strain CH1197/96 might be the first representative of a novel reptilian origin Orthoreovirus species.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143 Budapest, Hungary.
authors.forename: Renáta
authors.initials: R
authors.lastname: Kugler
authors.affiliation: LABOKLIN GmbH & Co. KG, Laboratory for Clinical Diagnostics, Steubenstr. 4, 97688 Bad Kissingen, Germany.
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Marschang
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143 Budapest, Hungary.
authors.forename: Katalin
authors.initials: K
authors.lastname: Ihász
authors.affiliation: Medical Centre of Hungarian Defense Forces, Force Health Laboratory Institute, Róbert Károly krt. 44, 1134 Budapest, Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Lengyel
authors.affiliation: János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs 7624, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Jakab
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143 Budapest, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143 Budapest, Hungary. Electronic address: fszilvi@yahoo.com.
authors.forename: Szilvia L
authors.initials: SL
authors.lastname: Farkas
mesh_terms: D000818:Animals; D014644:Genetic Variation; D016679:Genome, Viral; D018165:Orthoreovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014426:Turtles / Q000821:virology*
keywords: Orthoreovirus; Phylogenetic analysis; Reoviridae; Testudo graeca; Virus taxonomy