title: Hepatoprotective activity of white horehound (Marrubium vulgare) extract against cyclophosphamide toxicity in male rats.
abstract: The hepatoprotective activity of Marrubium vulgare against cyclophosphamide toxicity in Wistar rats was evaluated. Adult male rats were divided into 4 groups of 6 each: a control group, a group injected with cyclophosphamide (150 mg·kg(-1)) for 3 days, a group orally given a M. vulgare aqueous extract ((500 mg of dry leaves)·kg(-1)·day(-1)) for 30 days then treated with cyclophosphamide, and a group receiving only M. vulgare for 30 days. After 33 days of treatment, activities of alanine amino transferase (ALAT), aspartate amino transferase (ASAT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) were determined in serum. Moreover, lipid peroxidation level and superoxide dismutase (SOD) activities, catalase (CAT) and glutathione peroxidase (GPx) were measured in liver. Alterations of these hepatic biomarkers and increased lipid peroxidation confirmed cyclophosphamide-induced liver toxicity. Cyclophosphamide also decreased the enzymatic defense system against oxidative stress. However, when this drug was administered in rats given M. vulgare extract, all the biological parameters underwent much less alteration. Administration of M. vulgare extract was found to be beneficial by attenuating cyclophosphamide-induced liver damage. The protective effect of the plant is mainly attributed to its antioxidant properties and the existence of phenolic acids and flavonoids, as highlighted by HPLC-based analysis.
authors.affiliation: a Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia.|b Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Gafsa, 2100 Gafsa, Tunisia.
authors.forename: Amani
authors.initials: A
authors.lastname: Ettaya
authors.affiliation: a Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia.|b Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Gafsa, 2100 Gafsa, Tunisia.
authors.forename: Sabah
authors.initials: S
authors.lastname: Dhibi
authors.affiliation: a Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia.|b Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Gafsa, 2100 Gafsa, Tunisia.
authors.forename: Noura
authors.initials: N
authors.lastname: Samout
authors.affiliation: a Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia.
authors.forename: Abdelfettah
authors.initials: A
authors.lastname: Elfeki
authors.affiliation: a Laboratory of Environmental Physiopathology, Valorization of Bioactive Molecules and Mathematical Modeling, Faculty of Sciences of Sfax, Road Soukra km 3.5- PB n° 1171-3000, Sfax, Tunisia.|b Laboratory of Animal Ecophysiology, Faculty of Sciences, University of Gafsa, 2100 Gafsa, Tunisia.
authors.forename: Najla
authors.initials: N
authors.lastname: Hfaiedh
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D003520:Cyclophosphamide / Q000009:adverse effects*; D005419:Flavonoids / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D027522:Marrubium / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D020011:Protective Agents; D003520:Cyclophosphamide; D002251:Carbon Tetrachloride; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C508337:Marrubium vulgare extract
keywords: Marrubium vulgare; cyclophosphamide; foie; liver; oxidative stress; stress oxydatif