title: Characterization of Enterococcus faecium bacteriophage IME-EFm5 and its endolysin LysEFm5.
abstract: Due to the worldwide prevalence of antibiotic resistant strains, phages therapy has been revitalized recently. In this study, an Enterococcus faecium phage named IME-EFm5 was isolated from hospital sewage. Whole genomic sequence analysis demonstrated that IME-EFm5 belong to the Siphoviridae family, and has a double-stranded genome of 42,265bp (with a 35.51% G+C content) which contains 70 putative coding sequences. LysEFm5, the endolysin of IME-EFm5, contains an amidase domain in its N-terminal and has a wider bactericidal spectrum than its parental phage IME-EFm5, including 7 strains of vancomycin-resistant E. faecium. The mutagenesis analysis revealed that the zinc ion binding residues (H27, H132, and C140), E90, and T138 are required for the catalysis of LysEFm5. However, the antibacterial activity of LysEFm5 is zinc ion independent, which is inconsistent with most of other amidase members. The phage lysin LysEFm5 might be an alternative treatment strategy for infections caused by multidrug-resistant E. faecium.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Gong
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Mengjun
authors.initials: M
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Li
authors.affiliation: The first affiliated hospital to Changchun University of Chinese Medicine, Changchun 130021, PR China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Nadire
authors.initials: N
authors.lastname: Kahaer
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Juecheng
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Xia
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, PR China. Electronic address: jingmin0629@163.com.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Gu
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016984:Enterococcus faecium / Q000302:isolation & purification / Q000821:virology*; D015870:Gene Expression; D059646:Genome Size; D016679:Genome, Viral; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D012722:Sewage / Q000821:virology; D017906:Siphoviridae / Q000201:enzymology / Q000235:genetics*; D020713:Vancomycin Resistance / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D012722:Sewage; D014764:Viral Proteins; D010450:Endopeptidases; C001143:endolysin; D000581:Amidohydrolases; D015032:Zinc
keywords: Amidase; Enterococcus faecium; Phage lysin; Zinc-dependent