title: Bioactivity of a Rice Bran-Derived Peptide and its Sensory Evaluation and Storage Stability in Orange Juice.
abstract: A pentapeptide prepared from rice bran demonstrated growth inhibition on human lung, liver, breast, and colon cancer cell lines. The objectives of this study were to evaluate the human prostate cancer growth inhibition by the pentapeptide and its 6-mo storage stability by incorporating spray-dried orange juice, and determining sensory acceptability. The pentapeptide showed inhibition of human prostate cancer cells by 45% at 460 μg/mL concentration. When incorporated in spray-dried orange juice, and reconstituted with water and tested, there was an approximately 10% degradation of the peptide at 620 μg/mL concentration under refrigerated conditions over a 6 mo storage period, whereas at ambient temperature the degradation was 30%. Larger degradation was observed when 240 or 460 μg/mL pentapeptide was used. Overall, consumer panelists liked sensory aspect of the reconstituted pentapeptide incorporated orange juice beverage. Also consumer panelists liked the color and mouthfeel attributes, their hedonic impression of flavor attribute was slightly low due to unpalatable bitter note caused by the presence of the peptide. Incorporation of the pentapeptide in spray-dried orange juice has the potential to serve as a functional food ingredient that can offer health benefits to consumers. It is possible that the structural instability can be minimized by encapsulation.
authors.affiliation: Simmons Foods Inc, 601 N Hico St., Siloam Springs, AR, 72761, U.S.A.
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Graves
authors.affiliation: Food Science Dept, Univ. of Arkansas, 2650 N Young Ave., Fayetteville, AR, 72704, U.S.A.
authors.forename: Navam
authors.initials: N
authors.lastname: Hettiarachchy
authors.affiliation: Food Science Dept, Univ. of Arkansas, 2650 N Young Ave., Fayetteville, AR, 72704, U.S.A.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Rayaprolu
authors.affiliation: Dept. of Food Science and Nutrition, Univ. of Florida, 572 Newell Drive, Gainesville, FL, 32601, U.S.A.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Li
authors.affiliation: Food Science Dept, Univ. of Arkansas, 2650 N Young Ave., Fayetteville, AR, 72704, U.S.A.
authors.forename: Ronny
authors.initials: R
authors.lastname: Horax
authors.affiliation: Food Science Dept, Univ. of Arkansas, 2650 N Young Ave., Fayetteville, AR, 72704, U.S.A.
authors.forename: Han-Seok
authors.initials: HS
authors.lastname: Seo
mesh_terms: D045744:Cell Line, Tumor; D032084:Citrus sinensis; D003116:Color; D003258:Consumer Behavior; D002523:Edible Grain / Q000737:chemistry; D005511:Food Handling / Q000379:methods; D061353:Food Storage / Q000379:methods*; D000067030:Fruit and Vegetable Juices; D006801:Humans; D008297:Male; D012275:Oryza / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D011471:Prostatic Neoplasms / Q000178:diet therapy* / Q000188:drug therapy; D059748:Proteolysis; D012034:Refrigeration; D013649:Taste; D013696:Temperature
chemical_list: D010455:Peptides
keywords: bioactive peptide; cancer; functional beverage; rice bran; sensory; storage stability