title: Human neutrophil peptide-1 affects matrix metalloproteinase-2, -8 and -9 secretions of oral squamous cell carcinoma cell lines in vitro.
abstract: OBJECTIVE
  The present study aimed to investigate the effect of HNP-1 on the matrix metalloproteinase (MMP)-2, -8 and -9 secretions of two oral squamous cell carcinoma (OSCC) cell lines (UT-SCC-43A and UT-SCC-43B).
  
  
  METHODS
  In all experiments, the two OSCC cell lines were incubated with graded concentrations (0, 1, 5, and 10 μg/ml) of HNP-1 for 24 and 48 h. Cell viability was measured using a colorimetric proliferation test and cell death was analyzed with a colorimetric cytotoxicity detection kit. Enzyme activity of MMP-2 and MMP-9 was detected by using gelatin zymography, and molecular weight forms of MMP-8 were determined by Western-blot and a densitometric quantitation method.
  
  
  RESULTS
  Both cell lines showed a significant increase in LDH toxicity at 24h (UT-SCC-43A: p=0.005 & UT-SCC-43B: p=0.014). Reduced gelatinolytic activities of proMMP-2 were detected in UT-SCC-43B cell line after 24 and 48 h of incubation with HNP-1 (1 μg/ml: p<0.001, 5 μg/ml: p<0.001, and 10 μg/ml: p=0.0225). MMP-8 levels of both cell lines decreased at 200-250 kDa after 24h of incubation, while after 48 h only UT-SCC-43B decreased at 45-50 kDa.
  
  
  CONCLUSIONS
  Our results indicate that HNP-1 suppresses the secretion of MMP-2, -8, and -9 in OSCC cell lines.
authors.affiliation: Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland. Electronic address: ahsamu@utu.fi.
authors.forename: Ahmed Ali
authors.initials: AA
authors.lastname: Musrati
authors.affiliation: Departments of Oral and Maxillofacial Diseases, Helsinki University and Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Taina
authors.initials: T
authors.lastname: Tervahartiala
authors.affiliation: Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland.
authors.forename: Mervi
authors.initials: M
authors.lastname: Gürsoy
authors.affiliation: Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland.
authors.forename: Eija
authors.initials: E
authors.lastname: Könönen
authors.affiliation: Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland.
authors.forename: Dareen
authors.initials: D
authors.lastname: Fteita
authors.affiliation: Departments of Oral and Maxillofacial Diseases, Helsinki University and Helsinki University Central Hospital, Helsinki, Finland; Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
authors.affiliation: Departments of Oral and Maxillofacial Diseases, Helsinki University and Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: Veli-Jukka
authors.initials: VJ
authors.lastname: Uitto
authors.affiliation: Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland.
authors.forename: Ulvi Kahraman
authors.initials: UK
authors.lastname: Gürsoy
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003720:Densitometry / Q000379:methods; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020782:Matrix Metalloproteinases / Q000096:biosynthesis / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D000077195:Squamous Cell Carcinoma of Head and Neck; D023084:alpha-Defensins / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D004792:Enzyme Precursors; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; D005780:Gelatin; D018093:Gelatinases; D020782:Matrix Metalloproteinases; C069860:progelatinase
keywords: Antimicrobial peptide; In vitro experiment; MMPs; Oral cancer