title: Phylogenetic analysis of Dengue virus 1 isolated from South Minas Gerais, Brazil.
abstract: Dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. Primary infection with a single Dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease, called dengue hemorrhagic fever. The four Dengue virus serotypes (1-4) are antigenically and genetically distinct and each serotype is composed of multiple genotypes. In this study we isolated one Dengue virus 1 serotype, named BR/Alfenas/2012, from a patient with dengue hemorrhagic fever in Alfenas, South Minas Gerais, Brazil and molecular identification was performed based on the analysis of NS5 gene. Swiss mice were infected with this isolate to verify its potential to induce histopathological alterations characteristic of dengue. Liver histopathological analysis of infected animals showed the presence of inflammatory infiltrates, hepatic steatosis, as well as edema, hemorrhage and necrosis focal points. Phylogenetic and evolutionary analyses based on the envelope gene provided evidence that the isolate BR/Alfenas/2012 belongs to genotype V, lineage I and it is probably derived from isolates of Rio de Janeiro, Brazil. The isolate BR/Alfenas/2012 showed two unique amino acids substitutions (SER222THRE and PHE306SER) when compared to other Brazilian isolates from the same genotype/lineage. Molecular models were generated for the envelope protein indicating that the amino acid alteration PHE 306 SER could contribute to a different folding in this region located within the domain III. Further genetic and animal model studies using BR/Alfenas/2012 and other isolates belonging to the same lineage/genotype could help determine the relation of these genetic alterations and dengue hemorrhagic fever in a susceptible population.
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
authors.forename: Betania Paiva
authors.initials: BP
authors.lastname: Drumond
authors.affiliation: Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
authors.forename: Luiz Gustavo da Silva
authors.initials: LG
authors.lastname: Fagundes
authors.affiliation: Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
authors.forename: Raissa Prado
authors.initials: RP
authors.lastname: Rocha
authors.affiliation: Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
authors.forename: Marcilio Jorge
authors.initials: MJ
authors.lastname: Fumagalli
authors.affiliation: Laboratório de Pesquisas Em Virologia, Departamento de Doenças Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São Paulo, Brazil.
authors.forename: Carlos Shigueru
authors.initials: CS
authors.lastname: Araki
authors.affiliation: Laboratório de Pesquisas Em Virologia, Departamento de Doenças Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São Paulo, Brazil.
authors.forename: Tatiana Elisa
authors.initials: TE
authors.lastname: Colombo
authors.affiliation: Laboratório de Pesquisas Em Virologia, Departamento de Doenças Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São Paulo, Brazil.
authors.forename: Mauricio Lacerda
authors.initials: ML
authors.lastname: Nogueira
authors.affiliation: Laboratório de Bioinformática, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
authors.forename: Thiago Elias
authors.initials: TE
authors.lastname: Castilho
authors.affiliation: Laboratório de Bioinformática, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
authors.forename: Nelson José Freitas
authors.initials: NJ
authors.lastname: da Silveira
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.
authors.forename: Luiz Cosme Cotta
authors.initials: LC
authors.lastname: Malaquias
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil. Electronic address: coelho@unifal-mg.edu.br.
authors.forename: Luiz Felipe Leomil
authors.initials: LF
authors.lastname: Coelho
mesh_terms: D019943:Amino Acid Substitution; D000825:Animal Structures / Q000473:pathology; D000818:Animals; D001938:Brazil; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004195:Disease Models, Animal; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006651:Histocytochemistry; D006801:Humans; D051379:Mice; D008853:Microscopy; D008958:Models, Molecular; D010802:Phylogeny; D017354:Point Mutation; D011487:Protein Conformation; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D015686:Gene Products, env; C582223:NS5 protein, dengue virus; D017361:Viral Nonstructural Proteins
keywords: Alfenas; Dengue virus-1; Minas Gerais; Phylogenetic analysis