title: Development and Characterization of Monoclonal Antibodies Specific for 3-(1-naphthoyl) Indole Derivatives.
abstract: 3-(1-naphthoyl) indole is one of the raw materials that synthesizes a synthetic cannabinoid such as 1-pentyl-3-(1-naphthoyl) indole (JWH-018) and 1-butyl-3-(1-naphthoyl) indole (JWH-073). It is important to detect the 3-(1-naphthoyl) indole derivatives rapidly, sensitively, and comprehensively. We developed two monoclonal antibodies (MAb) against 3-(1-naphthoyl) indole derivatives, named NT1 (IgG1) and NT2 (IgG1), which were possibly effective for detecting 3-(1-naphthoyl) indole derivatives. The cross-reactive ability of these MAbs was evaluated using a competitive enzyme-linked immunosorbent assay (ELISA). In the results, we found both of these antibodies recognize 3-(1-naphthoyl) indole and its derivatives. However neither of these antibodies recognize naphtoic acid, 4-methyl-naphtoic acid, and indole. Sixty to 100 nanomole per liter of 3-(1-naphthoyl) indole derivatives, such as 1-methyl-3-(1-naphthoyl) indole, 1-ethyl-3-(1-naphthoyl) indole, and 1-octyl-3-(1-naphthoyl) indole, can be detected using both of the obtained MAbs. Thus, the MAbs produced in this study could be a useful tool for the detection of 3-(1-naphthoyl) indole derivatives.
authors.affiliation: 1 Bioscience Technology Development Office, R&D Division, Panasonic Company , Soraku-gun, Kyoto-fu, Japan .|2 Graduate School of Science and Engineering, Kagoshima University , Kagoshima, Japan .
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakayama
authors.affiliation: 1 Bioscience Technology Development Office, R&D Division, Panasonic Company , Soraku-gun, Kyoto-fu, Japan .
authors.forename: Noriko
authors.initials: N
authors.lastname: Kenjyou
authors.affiliation: 2 Graduate School of Science and Engineering, Kagoshima University , Kagoshima, Japan .
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D013287:Illicit Drugs / Q000276:immunology*; D007074:Immunoglobulin G; D007211:Indoles / Q000276:immunology*; D051379:Mice; D009281:Naphthalenes / Q000276:immunology
chemical_list: C000612031:1-ethyl-3-(1-naphthoyl)indole; C000612030:1-methyl-3-(1-naphthoyl)indole; C000612032:1-octyl-3-(1-naphthoyl)indole; D000911:Antibodies, Monoclonal; D013287:Illicit Drugs; D007074:Immunoglobulin G; D007211:Indoles; D009281:Naphthalenes; C568258:JWH-073; C552597:1-pentyl-3-(1-naphthoyl)indole