title: A dynamic look backward and forward.
abstract: The 2015 Gunther Laukien Prize recognized solution NMR studies of protein dynamics and thermodynamics. This Perspective surveys aspects of the development and application of NMR spin relaxation for investigations of protein flexibility and function over multiple time scales in solution. Methods highlighted include analysis of overall rotational diffusion, theoretical descriptions of R1ρ relaxation, and molecular dynamics simulations to interpret NMR spin relaxation. Applications are illustrated for the zinc-finger domain Xfin-31, the calcium-binding proteins calbindin D9k and calmodulin, and the bZip transcription factor of GCN4.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, United States.
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Palmer
mesh_terms: D000465:Algorithms; D005544:Forecasting; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D056004:Molecular Dynamics Simulation; D010449:Peptide Mapping / Q000379:methods* / Q000639:trends; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein / Q000379:methods* / Q000639:trends; D012815:Signal Processing, Computer-Assisted
chemical_list: D011506:Proteins
keywords: Chemical exchange; Conformational dynamics; Kinetics; Ligand binding; Molecular dynamics simulation; Nuclear magnetic resonance spectroscopy; Spin relaxation