title: Enhanced cellulase production by Trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates.
abstract: The use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. This work evaluates the production of cellulase enzymes using glycerol for high cell density growth of Trichoderma harzianum followed by induction with a cellulosic material. Firstly, the influence of the carbon source used in the pre-culture step was investigated in terms of total protein secretion and fungal morphology. Enzymatic productivity was then determined for cultivation strategies using different types and concentrations of carbon source, as well as different feeding procedures (batch and fed-batch). The best strategy for cellulase production was then further studied on a larger scale using a stirred tank bioreactor. The proposed strategy for cellulase production, using glycerol to achieve high cell density growth followed by induction with pretreated sugarcane bagasse, achieved enzymatic activities up to 2.27 ± 0.37 FPU/mL, 106.40 ± 8.87 IU/mL, and 9.04 ± 0.39 IU/mL of cellulase, xylanase, and β-glucosidase, respectively. These values were 2 times higher when compared to the control experiments using glucose instead of glycerol. This novel strategy proved to be a promising approach for improving cellulolytic enzymes production, and could potentially contribute to adding value to biomass within the biofuels sector.
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, CEP 13083-970, Brazil. priscila.delabona@bioetanol.org.br.|Graduate Program of Biotechnology, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil. priscila.delabona@bioetanol.org.br.
authors.forename: Priscila da Silva
authors.initials: Pda S
authors.lastname: Delabona
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, CEP 13083-970, Brazil.
authors.forename: Deise Juliana
authors.initials: DJ
authors.lastname: Lima
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, CEP 13083-970, Brazil.
authors.forename: Diogo
authors.initials: D
authors.lastname: Robl
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, CEP 13083-970, Brazil.
authors.forename: Sarita Cândida
authors.initials: SC
authors.lastname: Rabelo
authors.affiliation: Graduate Program of Biotechnology, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil.|Embrapa Instrumentation, Rua XV de Novembro 1452, São Carlos, SP, CEP 13560-970, Brazil.
authors.forename: Cristiane Sanchez
authors.initials: CS
authors.lastname: Farinas
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, CEP 13083-970, Brazil.|Graduate Program of Biotechnology, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil.
authors.forename: José Geraldo da Cruz
authors.initials: JG
authors.lastname: Pradella
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism* / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism* / Q000494:pharmacology; D031786:Saccharum / Q000737:chemistry; D014242:Trichoderma / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D056804:Biofuels; D002482:Cellulose; C027433:bagasse; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose; D005990:Glycerol
keywords: Biodiesel; Bioethanol; Cellulases; Enzymes; Gycerol; Pretreated bagasse; Trichoderma harzianum