title: Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice.
abstract: Matrine is isolated from Sophora flavescens and shows anti-inflammatory effects in macrophages. Here we evaluated matrine's suppressive effects on cyclooxygenase 2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) expressions in lipopolysaccharide- (LPS-) stimulated human lung epithelial A549 cells. Additionally, BALB/c mice were given various matrine doses by intraperitoneal injection, and then lung injury was induced via intratracheal instillation of LPS. In LPS-stimulated A549 cells, matrine inhibited the productions of interleukin-8 (IL-8), monocyte chemotactic protein-1, and IL-6 and decreased COX-2 expression. Matrine treatment also decreased ICAM-1 protein expression and suppressed the adhesion of neutrophil-like cells to inflammatory A549 cells. In vitro results demonstrated that matrine significantly inhibited mitogen-activated protein kinase phosphorylation and decreased nuclear transcription factor kappa-B subunit p65 protein translocation into the nucleus. In vivo data indicated that matrine significantly inhibited neutrophil infiltration and suppressed productions of tumor necrosis factor-α and IL-6 in mouse bronchoalveolar lavage fluid and serum. Analysis of lung tissue showed that matrine decreased the gene expression of proinflammatory cytokines, chemokines, COX-2, and ICAM-1. Our findings suggest that matrine improved lung injury in mice and decreased the inflammatory response in human lung epithelial cells.
authors.affiliation: Department of Nursing, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan; Chang Gung Memorial Hospital at Linkou, Guishan District, Taoyuan City 33303, Taiwan.
authors.forename: Chian-Jiun
authors.initials: CJ
authors.lastname: Liou
authors.affiliation: Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan.
authors.forename: You-Rong
authors.initials: YR
authors.lastname: Lai
authors.affiliation: Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan.
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan.
authors.forename: Yi-Hsien
authors.initials: YH
authors.lastname: Chang
authors.affiliation: Chang Gung Memorial Hospital at Linkou, Guishan District, Taoyuan City 33303, Taiwan.
authors.forename: Zih-Ying
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Chang Gung Memorial Hospital at Linkou, Guishan District, Taoyuan City 33303, Taiwan; Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan; Graduate Institute of Health Industry Technology, Chang Gung University of Science Taiwan and Technology, No. 261 Wenhua 1st Road, Guishan District, Taoyuan City 33303, Taiwan.
authors.forename: Wen-Chung
authors.initials: WC
authors.lastname: Huang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000188:drug therapy*; D000470:Alkaloids / Q000627:therapeutic use*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011807:Quinolizines / Q000627:therapeutic use*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D008070:Lipopolysaccharides; D011807:Quinolizines; D018799:Intercellular Adhesion Molecule-1; D051546:Cyclooxygenase 2; D000093842:Matrines