title: Luminescent platinum(II) complexes with functionalized N-heterocyclic carbene or diphosphine selectively probe mismatched and abasic DNA.
abstract: The selective targeting of mismatched DNA overexpressed in cancer cells is an appealing strategy in designing cancer diagnosis and therapy protocols. Few luminescent probes that specifically detect intracellular mismatched DNA have been reported. Here we used Pt(II) complexes with luminescence sensitive to subtle changes in the local environment and report several Pt(II) complexes that selectively bind to and identify DNA mismatches. We evaluated the complexes' DNA-binding characteristics by ultraviolet/visible absorption titration, isothermal titration calorimetry, nuclear magnetic resonance and quantum mechanics/molecular mechanics calculations. These Pt(II) complexes show up to 15-fold higher emission intensities upon binding to mismatched DNA over matched DNA and can be utilized for both detecting DNA abasic sites and identifying cancer cells and human tissue samples with different levels of mismatch repair. Our work highlights the potential of luminescent Pt(II) complexes to differentiate between normal cells and cancer cells which generally possess more aberrant DNA structures.
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials, Chemical Biology Centre, and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Sin Ki
authors.initials: SK
authors.lastname: Fung
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials, Chemical Biology Centre, and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.|HKU Shenzhen Institute of Research and Innovation Shenzhen, Shenzhen 518053, China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials, Chemical Biology Centre, and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Cao
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials, Chemical Biology Centre, and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Wai-Pong
authors.initials: WP
authors.lastname: To
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials, Chemical Biology Centre, and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.|HKU Shenzhen Institute of Research and Innovation Shenzhen, Shenzhen 518053, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials, Chemical Biology Centre, and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
authors.forename: Chun-Nam
authors.initials: CN
authors.lastname: Lok
authors.affiliation: State Key Laboratory of Synthetic Chemistry, Institute of Molecular Functional Materials, Chemical Biology Centre, and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China.|HKU Shenzhen Institute of Research and Innovation Shenzhen, Shenzhen 518053, China.
authors.forename: Chi-Ming
authors.initials: CM
authors.lastname: Che
mesh_terms: D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D049449:Luminescence; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D010984:Platinum / Q000737:chemistry*
chemical_list: C030011:carbene; D010984:Platinum; D004247:DNA; D008697:Methane