title: Immunohistochemical expression levels of p53 and eIF4E markers in histologically negative surgical margins, and their association with the clinical outcome of patients with head and neck squamous cell carcinoma.
abstract: Molecular markers can be used to identify residual cancer at the surgical margins of head and neck squamous cell carcinoma (HNSCC) and assist in evaluating the complete resection of the tumour. The purpose of the present study was to investigate the expression levels of prognostic molecular markers at the histological tumour free surgical margins. In the present clinical retrospective study, 24/48 patients were selected with negative surgical margins for further analysis with immunohistochemical staining. Contingency tables and Fisher's exact tests were used to investigate the association between the expression levels of p53 and eukaryotic translation imitation factor 4E (eIF4E) with the clinical outcomes for patients with HNSCC. The expression levels of p53 and eIF4E were 54.2 and 87.5%, respectively, in the surgical margins of patients with HNSCC. A total of 3/7 patients with recurrent cancer (42.8%) were identified with p53-positive margins, and 6 (85.7%) patients exhibited recurrence with eIF4e-positive margins. No statistically significant differences were identified for the recurrence risk between the overexpression of p53 and eIF4E in the surgical margins (P=0.88 and P=0.99, respectively). The eIF4E marker appears to be a more marked prognosticator compared with p53, as overexpression of eIF4E was identified in the margins of 6/7 patients with local recurrence.
authors.affiliation: School of Psychological and Clinical Sciences, Charles Darwin University, Darwin, NT 0815, Australia.
authors.forename: Jagtar
authors.initials: J
authors.lastname: Singh
authors.affiliation: School of Psychological and Clinical Sciences, Charles Darwin University, Darwin, NT 0815, Australia.
authors.forename: Rama
authors.initials: R
authors.lastname: Jayaraj
authors.affiliation: Genesis Health Care Bunbury, Bunbury, WA 6230, Australia.
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Baxi
authors.affiliation: Department of Anatomical Pathology, Royal Darwin Hospital, Darwin, NT 0810, Australia.
authors.forename: Mariana
authors.initials: M
authors.lastname: Mileva
authors.affiliation: Department of Anatomical Pathology, Royal Darwin Hospital, Darwin, NT 0810, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Skinner
authors.affiliation: Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia.
authors.forename: Navneet K
authors.initials: NK
authors.lastname: Dhand
authors.affiliation: Department of Head and Neck Surgery, Royal Darwin Hospital, Darwin, NT 0811, Australia.
authors.forename: Mahiban
authors.initials: M
authors.lastname: Thomas
keywords: eukaryotic translation imitation factor 4E; immunohistochemistry; molecular markers; overall survival; p53; recurrence