title: Immunoinformatic Analysis of Crimean Congo Hemorrhagic Fever Virus Glycoproteins and Epitope Prediction for Synthetic Peptide Vaccine.
abstract: OBJECTIVE
  To determine the Crimean Congo Hemorrhagic Fever (CCHF) virus M segement glycoprotein's immunoinformatic parameters, and identify Human Leukocyte Antigen (HLA) class I binders as candidates for synthetic peptide vaccines.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  Combined Military Hospital, Khuzdar Cantt, in May 2015.
  
  
  METHODS
  Data acquisition, antigenicity prediction, secondary and tertiary structure prediction, residue analysis were done using immunoinformatics tools. HLAclass I binders in glycoprotein's sequence were identified at nanomer length using NetMHC 3.4 and mapped onto tertiary structure. Docking was done for strongest binder against its corresponding allele with CABS-dock.
  
  
  RESULTS
  HLAA*0101, 0201, 0301, 2402, 2601 and B*0702, 0801, 2705, 3901, 4001, 5801, 1501 were analyzed against two glycoprotein components of the virus. Atotal of 35 nanomers from GP1, and 3 from GP2 were identified. HLAB*0702 bound maximum number of peptides (6), while HLAB*4001 showed strongest binding affinity.
  
  
  CONCLUSIONS
  HLAspecific glycoproteins epitope prediction can help identify synthetic peptide vaccine candidates.
authors.affiliation: Department of Immunology, Combined Military Hospital, Khuzdar Cantt.
authors.forename: Hamid Nawaz
authors.initials: HN
authors.lastname: Tipu
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D019295:Computational Biology / Q000379:methods*; D003430:Cross-Sectional Studies; D018604:Epitope Mapping; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006023:Glycoproteins / Q000378:metabolism*; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology*; D006801:Humans; D012367:RNA, Viral; D017422:Sequence Analysis, DNA; D022223:Vaccines, Subunit
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D012367:RNA, Viral; D022223:Vaccines, Subunit