title: PNA lectin for purifying mouse acinar cells from the inflamed pancreas.
abstract: Better methods for purifying human or mouse acinar cells without the need for genetic modification are needed. Such techniques would be advantageous for the specific study of certain mechanisms, such as acinar-to-beta-cell reprogramming and pancreatitis. Ulex Europaeus Agglutinin I (UEA-I) lectin has been used to label and isolate acinar cells from the pancreas. However, the purity of the UEA-I-positive cell fraction has not been fully evaluated. Here, we screened 20 widely used lectins for their binding specificity for major pancreatic cell types, and found that UEA-I and Peanut agglutinin (PNA) have a specific affinity for acinar cells in the mouse pancreas, with minimal affinity for other major pancreatic cell types including endocrine cells, duct cells and endothelial cells. Moreover, PNA-purified acinar cells were less contaminated with mesenchymal and inflammatory cells, compared to UEA-I purified acinar cells. Thus, UEA-I and PNA appear to be excellent lectins for pancreatic acinar cell purification. PNA may be a better choice in situations where mesenchymal cells or inflammatory cells are significantly increased in the pancreas, such as type 1 diabetes, pancreatitis and pancreatic cancer.
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Shane
authors.initials: S
authors.lastname: Fischbach
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Fusco
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Ray
authors.initials: R
authors.lastname: Zimmerman
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Zewen
authors.initials: Z
authors.lastname: Song
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Nebres
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: David Matthew
authors.initials: DM
authors.lastname: Ricks
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Krishna
authors.initials: K
authors.lastname: Prasadan
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shiota
authors.affiliation: Division of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: Sohail Z
authors.initials: SZ
authors.lastname: Husain
authors.affiliation: Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, 4401 Penn Ave, Pittsburgh, PA15224, USA.
authors.forename: George K
authors.initials: GK
authors.lastname: Gittes
mesh_terms: D061354:Acinar Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D051379:Mice; D010179:Pancreas / Q000166:cytology* / Q000473:pathology; D010195:Pancreatitis / Q000473:pathology*; D019887:Peanut Agglutinin / Q000378:metabolism
chemical_list: D019887:Peanut Agglutinin