title: Changes in expression of WT1 during induced differentiation of the acute myeloid leukemia cell lines by treatment with 5-aza-2'-deoxycytidine and all-transretinoic acid.
abstract: The aim of the present study was to investigate the effect of 5-aza-2'-deoxycytidine (decitabine; DAC) and all-transretinoic acid (ATRA) on Wilms' tumor 1 (WT1) in acute myeloid leukemia (AML)in vitro. The methylation status of the WT1 promoter was analyzed using methylation-specific polymerase chain reaction (MSP). The expression level of WT1 was detected by reverse transcription-quantitative polymerase chain reaction. The effect of DAC and ATRA on cell differentiation was evaluated by flow cytometry. The WT1 gene was methylated in U937 cells, but unmethylated in SHI-1 and K562 cells; the U937 cells did not express the WT1 gene, but the SHI-1 and K562 cells highly expressed the WT1 gene. DAC and ATRA, alone or in combination, exhibited no effect on the expression level of WT1 in the U937 cells and on the differentiation of the K562 cells. The combined treatment of DAC and ATRA markedly decreased the WT1 expression levels of the SHI-1 and K562 cells, and induced the differentiation of the SHI-1 and U937 cells. In the SHI-1 cells, WT1 expression changed inversely to the dynamic changes of cluster of differentiation 11b-positive rates. In conclusion, the combined treatment of DAC and ATRA has clinical therapeutic potential in acute monocytic leukemia patients with high WT1 expression and a poor response to standard induction chemotherapy.
authors.affiliation: Department of Hematology, The Center Hospital of Xuzhou, Xuzhou, Jiangsu, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Department of Urology, The Center Hospital of Xuzhou, Xuzhou, Jiangsu, P.R. China.
authors.forename: Jiahe
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Gu
authors.affiliation: Laboratory of China and United States Cooperation, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Comprehensive Laboratory, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wei
authors.affiliation: Hematology Laboratory, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Qiu
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, P.R. China.
authors.forename: Jiannong
authors.initials: J
authors.lastname: Cen
authors.affiliation: Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Xie
authors.affiliation: Jiangsu Institute of Hematology, The First Affiliated Hospital of Suzhou University, Suzhou, Jiangsu, P.R. China.
authors.forename: Zixing
authors.initials: Z
authors.lastname: Chen
keywords: 5-aza-2′-deoxycytidine; WT1 gene; acute myeloid leukemia; all-trans retinoic acid; differentiation