title: Discovery of New Substrates for LuxAB Bacterial Bioluminescence.
abstract: In this article, four novel substrates with long halftime have been designed and synthesized successfully for luxAB bacterial bioluminescence. After in vitro and in vivo biological evaluation, these molecules can emit obvious bioluminescence emission with known bacterial luciferase, thus indicating a new promising approach to developing the bacterial bioluminescent system.
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Weishan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Natural Products, Key Laboratory of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Xingkang
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Haixiu
authors.initials: H
authors.lastname: Bai
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Natural Products, Key Laboratory of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Yuemao
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Keqian
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Minyong
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D049449:Luminescence; D051379:Mice; D008819:Mice, Nude; D066244:Proton Magnetic Resonance Spectroscopy; D013050:Spectrometry, Fluorescence; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins
keywords: bacterial bioluminescence; imaging; luxAB; new long-chain aliphatic aldehydes