title: A DNA aptamer recognising a malaria protein biomarker can function as part of a DNA origami assembly.
abstract: DNA aptamers have potential for disease diagnosis and as therapeutics, particularly when interfaced with programmable molecular technology. Here we have combined DNA aptamers specific for the malaria biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) with a DNA origami scaffold. Twelve aptamers that recognise PfLDH were integrated into a rectangular DNA origami and atomic force microscopy demonstrated that the incorporated aptamers preserve their ability to specifically bind target protein. Captured PfLDH retained enzymatic activity and protein-aptamer binding was observed dynamically using high-speed AFM. This work demonstrates the ability of DNA aptamers to recognise a malaria biomarker whilst being integrated within a supramolecular DNA scaffold, opening new possibilities for malaria diagnostic approaches based on DNA nanotechnology.
authors.affiliation: Heddle Initiative Research Unit, RIKEN, Saitama, 351-0198, Japan.|Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku, Tokyo 162-8480, Japan.
authors.forename: Maia
authors.initials: M
authors.lastname: Godonoga
authors.affiliation: Heddle Initiative Research Unit, RIKEN, Saitama, 351-0198, Japan.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Lin
authors.affiliation: NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Oshima
authors.affiliation: NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Sumitomo
authors.affiliation: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
authors.forename: Marco S L
authors.initials: MSL
authors.lastname: Tang
authors.affiliation: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
authors.forename: Yee-Wai
authors.initials: YW
authors.lastname: Cheung
authors.affiliation: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Kinghorn
authors.affiliation: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
authors.forename: Roderick M
authors.initials: RM
authors.lastname: Dirkzwager
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, No. 301 Xuefu Road, Zhenjiang 212013, China.
authors.forename: Cunshan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Chem. Mater. Eng., Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Kuzuya
authors.affiliation: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
authors.forename: Julian A
authors.initials: JA
authors.lastname: Tanner
authors.affiliation: Heddle Initiative Research Unit, RIKEN, Saitama, 351-0198, Japan.|Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Krakow, Poland.
authors.forename: Jonathan G
authors.initials: JG
authors.lastname: Heddle
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001483:Base Sequence; D015415:Biomarkers; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology*; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000469:parasitology; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D010963:Plasmodium falciparum / Q000235:genetics; D011485:Protein Binding; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D015800:Protozoan Proteins; D007770:L-Lactate Dehydrogenase