title: The establishment of species-specific primers for the molecular identification of ten stored-product psocids based on ITS2 rDNA.
abstract: Psocids are important stored product pests found worldwide that can be spread through grain trade. Most stored-product psocids, including eggs, nymphs, and adults, are very small (~1 mm) and difficult to identify morphologically. Here, we collected 10 economically important stored-product Liposcelis spp. psocids (L. bostrychophila, L. entomophila, L. decolor, L. paeta, L. brunnea, L. corrodens, L. mendax, L. rufa, L. pearmani, and L. tricolor) from 35 geographical locations in 5 countries (China, Czech Republic, Denmark, Germany, and the United States). The ITS2 rDNA gene was extracted and sequenced. The interspecific genetic distance of the stored-product psocids was significantly higher than the intraspecific genetic distance according to the barcoding gap analysis. Ten pairs of species-specific primers based on the ITS2 rDNA were developed for psocid identification. The sensitivity estimation indicated that the species-specific primers could correctly amplify the target ITS2 gene and successfully identify psocids at 1.0 ng/mL. Additionally, these species-specific primers could quantify specificity and identify 10 stored-product psocids; this approach could also be used to accurately identify other stored-product psocids. This work provides a practical approach for the precise examination of 10 stored-product psocid species and also contributes to the development of an identification method using ITS2 rDNA.
authors.affiliation: Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
authors.forename: Zi-Hua
authors.initials: ZH
authors.lastname: Zhao
authors.affiliation: Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
authors.forename: Bing-Yi
authors.initials: BY
authors.lastname: Cui
authors.affiliation: Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Li
authors.affiliation: Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.|Institute of Plant Quarantine Research, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.|College of Life Science, China Jiliang University, Hangzhou 310018, China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Yang
authors.affiliation: Crop Research Institute, Drnovská 507, 161 06 Prague 6, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kučerová
authors.affiliation: Crop Research Institute, Drnovská 507, 161 06 Prague 6, Czech Republic.
authors.forename: Václav
authors.initials: V
authors.lastname: Stejskal
authors.affiliation: Department of Entomology and Plant Pathology, 127 Noble Research Center, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: George
authors.initials: G
authors.lastname: Opit
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, China.
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Li
mesh_terms: D000818:Animals; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D019143:Evolution, Molecular; D007313:Insecta / Q000145:classification* / Q000235:genetics*; D013045:Species Specificity
chemical_list: D021901:DNA, Intergenic; D004275:DNA, Ribosomal