title: Immunomodulatory Effects of Nontoxic Glycoprotein Fraction Isolated from Rice Bran.
abstract: Rice bran, a by-product of brown rice milling, is a rich source of dietary fiber and protein, and its usage as a functional food is expected to increase. In this study, immunomodulatory effects of glycoprotein obtained from rice bran were studied in normal mice and mouse models of cyclophosphamide-induced immunosuppression. We prepared glycoprotein from rice bran by using ammonium precipitation and anion chromatography techniques. Different doses of glycoprotein from rice bran (10, 25, and 50 mg/kg) were administered orally for 28 days. On day 21, cyclophosphamide at a dose of 100 mg/kg was administered intraperitoneally. Glycoprotein from rice bran showed a significant dose-dependent restoration of the spleen index and white blood cell count in the immunocompromised mice. Glycoprotein from rice bran affected the immunomodulatory function by inducing the proliferation of splenic lymphocytes, which produce potential T and B cells. Moreover, it prevented cyclophosphamide-induced damage of Th1-type immunomodulatory function through enhanced secretion of Th1-type cytokines (interferon-γ and interleukin-12). These results indicate that glycoprotein from rice bran significantly recovered cyclophosphamide-induced immunosuppression. Based on these data, it was concluded that glycoprotein from rice bran is a potent immunomodulator and can be developed to recover the immunity of immunocompromised individuals.
authors.affiliation: Division of Functional Food Research, Korea Food Research Institute, Gyeonggi, South Korea.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Park
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute, Gyeonggi, South Korea.
authors.forename: A-Reum
authors.initials: AR
authors.lastname: Yu
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute, Gyeonggi, South Korea.
authors.forename: Hee-Do
authors.initials: HD
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Chung-Ang University, Seoul, South Korea.
authors.forename: Ha Hyung
authors.initials: HH
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Korea University, Seoul, South Korea.
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute, Gyeonggi, South Korea.
authors.forename: Hee-Don
authors.initials: HD
authors.lastname: Choi
mesh_terms: D000818:Animals; D004043:Dietary Fiber; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D007108:Immune Tolerance / Q000187:drug effects; D007155:Immunologic Factors / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza / Q000737:chemistry*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D004043:Dietary Fiber; D006023:Glycoproteins; D007155:Immunologic Factors