title: Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy.
abstract: Non-specific lipid transfer proteins (nsLTP) are considered to provoke allergic symptoms to plane tree pollen, which are frequently associated with peach allergy.
  
  
  
  The objective was to clone the cDNA of plane pollen nsLTP Pla a 3, to characterize IgE-binding and allergenic potency of recombinant Pla a 3 in comparison to its natural counterpart and peach nsLTP Pru p 3.
  
  
  
  Natural Pla a 3 was purified from plane pollen and analysed by mass spectrometry (MS). Recombinant Pla a 3 was characterized by SDS-PAGE and CD spectroscopy. Specific IgE to extract, components of plane pollen and Pru p 3 was measured by ImmunoCAP in sera of patients allergic to either plane pollen (n = 10), peach (n = 15) or both (n = 15). Biological potency of the proteins was investigated by in vitro mediator release assays and IgE cross-reactivity by competitive ELISA.
  
  
  
  Two Pla a 3 isoforms were identified. Recombinant Pla a 3 showed high purity, structural integrity, IgE-binding capacity comparable to nPla a 3 and biological potency. Sensitization to plane pollen extract was confirmed in 24/25 plane pollen allergics. The frequency of sensitization to Pla a 3 was 53% among patients allergic to both plane pollen and peach and 10% among plane pollen allergics tolerating peach where most patients were sensitized to Pla a 1. Pla a 3 and Pru p 3 showed strong bi-directional IgE cross-reactivity in patients allergic to peach and plane pollen, but not in peach allergics tolerating plane pollen. Levels of IgE-binding were generally higher to Pru p 3 than to Pla a 3.
  
  
  
  Sensitization to Pla a 3 is relevant in a subgroup of plane pollen allergics with concomitant peach allergy. IgE testing with Pla a 3 may serve as a marker to identify plane pollen allergic patients at risk of LTP-mediated food reactions and thereby improve in vitro diagnostic procedures.
authors.affiliation: VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Wangorsch
authors.affiliation: ThermoFisher Scientific, Uppsala, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Larsson
authors.affiliation: VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Messmer
authors.affiliation: Allergy Unit, Pneumology Department, Hospital Clinic, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: García-Moral
authors.affiliation: Test and Therapy Allergens, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Lauer
authors.affiliation: VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wolfheimer
authors.affiliation: VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schülke
authors.affiliation: Allergy Unit, Pneumology Department, Hospital Clinic, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Bartra
authors.affiliation: VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Vieths
authors.affiliation: ThermoFisher Scientific, Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Lidholm
authors.affiliation: VPr Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Scheurer
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D015415:Biomarkers; D003001:Cloning, Molecular; D003429:Cross Reactions / Q000276:immunology; D005512:Food Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D015870:Gene Expression; D006636:Histamine Release; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D010641:Phenotype; D011058:Pollen / Q000276:immunology; D020033:Protein Isoforms; D000068239:Prunus persica / Q000009:adverse effects*; D011994:Recombinant Proteins
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D015415:Biomarkers; D020033:Protein Isoforms; D011994:Recombinant Proteins; D007073:Immunoglobulin E
keywords: LTP syndrome; Platanus allergy; Pla a 3; Pru p 3; peach allergy; plane pollen