title: Gmelina arborea Roxb. (Family: Verbenaceae) Extract Upregulates the β-Cell Regeneration in STZ Induced Diabetic Rats.
abstract: Gmelina arborea Roxb. (common name: Et-demata, Family: Verbenaceae) has been used traditionally in Sri Lanka as a remedy against diabetes mellitus. The objective of the present study was to evaluate antidiabetic mechanisms of the aqueous bark extract of G. arborea in streptozotocin induced (STZ) diabetic male Wistar rats. Aqueous bark extract of G. arborea (1.00 g/kg) and glibenclamide as the standard drug (0.50 mg/kg) were administered orally using a gavage to STZ diabetic rats (65 mg/kg, ip) for 30 days. The antidiabetic mechanisms of aqueous extract of G. arborea (1.00 g/kg) were determined at the end of the experiment. The fasting blood glucose concentration was significantly lowered and the serum insulin and C-peptide concentrations were increased by 57% and 39% in plant extract treated rats on day 30, respectively (p < 0.05). The histopathology and immunohistochemistry results of the plant extract treated group showed a regenerative effect on β-cells of the pancreas in diabetic rats. In addition, serum lipid parameters were improved in G. arborea extract treated diabetic rats. The results revealed that the aqueous stem bark extract of G. arborea (1.00 g/kg) showed beneficial effects against diabetes mellitus through upregulating the β-cell regeneration and biosynthesis of insulin in diabetic rats.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Ruhuna, 80000 Galle, Sri Lanka.
authors.forename: Anoja Priyadarshani
authors.initials: AP
authors.lastname: Attanayake
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Ruhuna, 80000 Galle, Sri Lanka.
authors.forename: Kamani Ayoma Perera Wijewardana
authors.initials: KA
authors.lastname: Jayatilaka
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Ruhuna, 80000 Galle, Sri Lanka.
authors.forename: Chitra
authors.initials: C
authors.lastname: Pathirana
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Ruhuna, 80000 Galle, Sri Lanka.
authors.forename: Lakmini Kumari Boralugoda
authors.initials: LK
authors.lastname: Mudduwa
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002096:C-Peptide / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008297:Male; D010179:Pancreas / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D013311:Streptozocin; D015854:Up-Regulation; D029648:Verbenaceae / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D002096:C-Peptide; D007004:Hypoglycemic Agents; D007328:Insulin; D008055:Lipids; D010936:Plant Extracts; D013311:Streptozocin