title: Hyaluronic acid modified mesoporous carbon nanoparticles for targeted drug delivery to CD44-overexpressing cancer cells.
abstract: In this paper, hyaluronic acid (HA) functionalized uniform mesoporous carbon spheres (UMCS) were synthesized for targeted enzyme responsive drug delivery using a facile electrostatic attraction strategy. This HA modification ensured stable drug encapsulation in mesoporous carbon nanoparticles in an extracellular environment while increasing colloidal stability, biocompatibility, cell-targeting ability, and controlled cargo release. The cellular uptake experiments of fluorescently labeled mesoporous carbon nanoparticles, with or without HA functionalization, demonstrated that HA-UMCS are able to specifically target cancer cells overexpressing CD44 receptors. Moreover, the cargo loaded doxorubicin (DOX) and verapamil (VER) exhibited a dual pH and hyaluronidase-1 responsive release in the tumor microenvironment. In addition, VER/DOX/HA-UMCS exhibited a superior therapeutic effect on an in vivo HCT-116 tumor in BALB/c nude mice. In summary, it is expected that HA-UMCS will offer a new method for targeted co-delivery of drugs to tumors overexpressing CD44 receptors.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, People's Republic of China. Department of Pharmacy, The First Affiliated Hospital of China Medical University, Nanajingbei Street 155, Shenyang, People's Republic of China. College of Pharmaceutical Science, China Medical University, Puhe Road 77, Shenyang 110122, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Wan
authors.forename: Jian
authors.initials: J
authors.lastname: Jiao
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
authors.forename: Jingwen
authors.initials: J
authors.lastname: Guo
authors.forename: Ning
authors.initials: N
authors.lastname: Han
authors.forename: Donghua
authors.initials: D
authors.lastname: Di
authors.forename: Di
authors.initials: D
authors.lastname: Chang
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.forename: Tongying
authors.initials: T
authors.lastname: Jiang
authors.forename: Siling
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy*; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D045325:HCT116 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000138:chemical synthesis*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D016062:Porosity; D014700:Verapamil / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D004317:Doxorubicin; D006820:Hyaluronic Acid; D014700:Verapamil