title: MiR-218 inhibits the tumorgenesis and proliferation of glioma cells by targeting Robo1.
abstract: BACKGROUND
  Malignant glioma is the most common primary brain tumors directly correlated with the high mortality and poor prognosis in clinical practice. MicroRNAs (miRNAs or miRs) influence numerous cancer-relevant processes including cell proliferation, differentiation and metabolism. However, the role of microRNA in malignant glioma is largely unknown. This study aimed to study the role of miR-218, a tumor-suppressive microRNA, in glioma development both in vivo and in vitro.
  
  
  METHODS
  The expression level of miR-218, Slit2 and Robo1 was examined by either quantitative (polymerase chain reaction) or western-blotting from both human glioma tissue and glioma cell lines. U87 cells were transfected with miR-218 and then the expression levels of Slit2 and Robo1 were quantified. Cell proliferation was measured both by the in vitro proliferation assay and in vivo graft studies. The luciferase reporter assay was employed to validate the downstream target of miR-218.
  
  
  RESULTS
  The expression of miR-218 was lower in glioma cell lines and glioma tissues from the patients with decreased Slit2 and increased Robo1 protein levels. The over-expression of miR-218 inhibited the tumorgenesis and proliferation of glioma cells remarkably. Furthermore, the over-expressing miR-218 in glioma cells results in the downregulation of Robo1 and upregulation of Slit2. Using luciferase reporter assays, we found that Robo1 was a direct downstream target of miR-218.
  
  
  CONCLUSIONS
  Over-expression of miR-218 in glioma cells may inhibit the proliferation and tumorigenicity through targeting Robo1, suggesting that miR-218 could be a potential target for developing therapies in treating glioma.
authors.affiliation: Department of Neurosurgery, Fuzhou General Hospital, Xiamen University Medical College, Fuzhou, Fujian, China.|Departments of Neurosurgery, Taizhou People's Hospital Affiliated to Medical College of The Nantong University, Taizhou, Jiangsu, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Gu
authors.affiliation: Departments of Neurosurgery, Taizhou People's Hospital Affiliated to Medical College of The Nantong University, Taizhou, Jiangsu, China.
authors.forename: Guang-Zhong
authors.initials: GZ
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009368:Neoplasm Transplantation; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D014183:Transplantation, Heterologous; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; D000098552:Slit Homolog 2 Protein; C547471:MIRN218 microRNA, human
keywords: Robo1; Slit2; gliomas; miR-218; proliferation; tumorgenesis