title: Layer-by-layer polysaccharide-coated liposomes for sustained delivery of epidermal growth factor.
abstract: A three-dimensional layer-by-layer (LbL) structure composed by xanthan and galactomannan biopolymers over dioctadecyldimethylammonium bromide (DODAB) liposome template was proposed and characterized for protein drug delivery. The polymers and the surfactant interaction were sufficiently strong to create a LbL structure up to 8 layers, evaluated using quartz crystal microbalance (QCM) and zeta potential analysis. The polymer-liposome binding enthalpy was determined by isothermal titration calorimetry (ITC). The bilayer of biopolymer-coated liposomes with diameters of 165 (±15)nm, measured by dynamic light scattering (DLS), and ζ-potential of -4 (±13)mV. These bilayer-coated nanoparticles increased up to 5 times the sustained release of epidermal growth factor (EGF) at a first order rate of 0.005min(-1). This system could be useful for improving the release profile of low-stability drugs like EGF.
authors.affiliation: BioPol, Chemistry Department, Universidade Federal do Paraná, R. Coronel F. H. dos Santos, Curitiba 210-81531-980, PR, Brazil; CEB, Pharmacy Department, Universidade Federal do Paraná, Av. Prof. Lothário Meissner, Curitiba 3400-80210-170, PR, Brazil.
authors.forename: Gabriel A T
authors.initials: GA
authors.lastname: Kaminski
authors.affiliation: BioPol, Chemistry Department, Universidade Federal do Paraná, R. Coronel F. H. dos Santos, Curitiba 210-81531-980, PR, Brazil.
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Sierakowski
authors.affiliation: CEB, Pharmacy Department, Universidade Federal do Paraná, Av. Prof. Lothário Meissner, Curitiba 3400-80210-170, PR, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pontarolo
authors.affiliation: BioPol, Chemistry Department, Universidade Federal do Paraná, R. Coronel F. H. dos Santos, Curitiba 210-81531-980, PR, Brazil.
authors.forename: Larissa Antoniacomi Dos
authors.initials: LA
authors.lastname: Santos
authors.affiliation: BioPol, Chemistry Department, Universidade Federal do Paraná, R. Coronel F. H. dos Santos, Curitiba 210-81531-980, PR, Brazil. Electronic address: rilton@ufpr.br.
authors.forename: Rilton Alves
authors.initials: RA
authors.lastname: de Freitas
mesh_terms: D003692:Delayed-Action Preparations; D065546:Drug Liberation; D004815:Epidermal Growth Factor / Q000008:administration & dosage* / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D008081:Liposomes; D008351:Mannans; D011135:Polysaccharides, Bacterial; D000644:Quaternary Ammonium Compounds; C026584:dioctadecyltrimethylammonium; C012990:galactomannan; D004815:Epidermal Growth Factor; C002563:xanthan gum; D005690:Galactose
keywords: DODAB; EGF; Galactomannan; Layer-by-layer; Liposomes; Xanthan