title: Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.
abstract: This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput).
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. fanbeiyuan@ucas.ac.cn.
authors.forename: Beiyuan
authors.initials: B
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. lixiufeng13@mails.ucas.ac.cn.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. dychen@mail.ie.ac.cn.
authors.forename: Deyong
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Science, Minzu University of China, Beijing 100081, China. hshpeng@muc.edu.cn.
authors.forename: Hongshang
authors.initials: H
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. jbwang@mail.ie.ac.cn.
authors.forename: Junbo
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. chenjian@mail.ie.ac.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
keywords: high-throughput; microfluidics; protein characterization; single-cell analysis