title: Suppression of mTOR Signaling Pathways in Skeletal Muscle of Finishing Pigs by Increasing the Ratios of Ether Extract and Neutral Detergent Fiber at the Expense of Starch in Iso-energetic Diets.
abstract: Three iso-energetic and iso-nitrogenous diets were fed to finishing pigs for 28 days to investigate the mammalian target of rapamycin (mTOR) and ubiquitin-proteasome signaling pathways of skeletal muscle by altering compositions of dietary energy sources. Diet A, diet B, and diet C contained 44.1%, 37.6%, and 30.9% starch; 5.9%, 9.5%, and 14.3% ether extract (EE); and 12.6%, 15.4%, and 17.8% neutral detergent fiber (NDF), respectively. An increase of mRNA expression of MuRF1 (1.09 ± 0.10 vs 1.00 ± 0.08) and MAFbx (1.10 ± 0.06 vs 1.00 ± 0.11) and a decrease of concentrations of plasma insulin (8.2 ± 0.8 vs 10.8 ± 1.2) and glucose (5.76 ± 0.12 vs 6.43 ± 0.33) together with mRNA expression of IRS (0.78 ± 0.19 vs 1.01 ± 0.05) and Akt (0.92 ± 0.01 vs 1.00 ± 0.05) were observed in pigs fed diet C compared with diet A. Protein levels of total and phosphorylated mTOR (0.31 ± 0.04 vs 0.48 ± 0.03 and 0.39 ± 0.01 vs 0.56 ± 0.02), 4EBP1 (0.66 ± 0.06 vs 0.90 ± 0.09 and 0.60 ± 0.12 vs 0.84 ± 0.09), and S6K1 (0.66 ± 0.01 vs 0.89 ± 0.01 and 0.48 ± 0.03 vs 0.79 ± 0.02) were decreased; however, total and phosphorylated AMPK (0.96 ± 0.06 vs 0.64 ± 0.04 and 0.97 ± 0.09 vs 0.61 ± 0.09) were increased in pigs fed diet C compared with diet A. In conclusion, diet C suppressed the mTOR pathway and accelerated the ubiquitin-proteasome pathway in skeletal muscle of finishing pigs.
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Changning
authors.initials: C
authors.lastname: Yu
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Yanjiao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Bolin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Meng
authors.initials: M
authors.lastname: Lin
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Jiaolong
authors.initials: J
authors.lastname: Li
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D018482:Muscle, Skeletal / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction; D013213:Starch / Q000378:metabolism*; D013552:Swine / Q000235:genetics / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D013213:Starch; D058570:TOR Serine-Threonine Kinases
keywords: dietary energy sources; finishing pigs; mTOR pathway; ubiquitin−proteasome pathway