title: Polyamines regulate cell growth and cellular methylglyoxal in high-glucose medium independently of intracellular glutathione.
abstract: Polyamines can presumably inhibit protein glycation, when associated with the methylglyoxal inevitably produced during glycolysis. Herein, we hypothesized a nonenzymatic interaction between putrescine and methylglyoxal in putrescine-deficient or -overexpressing Dictyostelium cells in high-glucose medium, which can control methylglyoxal production. Putrescine was essentially required for growth rescue accompanying methylglyoxal detoxification when cells underwent growth defect and cell cycle G1-arrest when supplemented with high glucose. Furthermore, methylglyoxal regulation by putrescine seemed to be a parallel pathway independent of the changes in cellular glutathione content in high-glucose medium. Consequently, we suggest that Dictyostelium cells need polyamines for normal growth and cellular methylglyoxal regulation.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kwak
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Mun-Hyoung
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Seong-Jun
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Shin
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, Institute of Microbiology, Seoul National University, Korea.
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D003470:Culture Media; D004023:Dictyostelium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D059585:G1 Phase Cell Cycle Checkpoints; D017125:Genes, Protozoan; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism; D011765:Pyruvaldehyde / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011073:Polyamines; D015800:Protozoan Proteins; D011765:Pyruvaldehyde; D009955:Ornithine Decarboxylase; D005978:Glutathione; D005947:Glucose; D011700:Putrescine
keywords: Dictyostelium discoideum; glutathione; methylglyoxal; polyamines; putrescine