title: Enzymatic saccharification of high pressure assist-alkali pretreated cotton stalk and structural characterization.
abstract: Cotton stalk is a potential biomass for bioethanol production, while the conversion of direct saccharification or biotransformation of cotton stalk is extremely low due to the recalcitrant nature of lignocellulose. To enhance the enzymatic conversion of cotton stalks, the enzymatic saccharification parameters of high pressure assist-alkali pretreatment (HPAP) cotton stalk were optimized in the present study. Results indicated that a maximum reducing sugar yield of 54.7g/100g dry biomass cellulose was achieved at a substrate concentration of 2%, 100rpm agitation, 0.6g/g enzyme loading, 40°C hydrolysis temperature, 50h saccharification time, and pH 5.0. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy were used to identify structural changes in native, pretreated biomass and hydrolyzed residues. Structural analysis revealed large part of amorphous cellulose and partial crystalline cellulose in the HPAP cotton stalk were hydrolyzed during enzymatic treatment. HPAP cotton stalk can be used as a potential feed stock for bioethanol production.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: dushuangkui@hotamil.com.
authors.forename: Shuang-kui
authors.initials: SK
authors.lastname: Du
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Su
authors.affiliation: Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Yang
authors.affiliation: Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Kuang
authors.affiliation: Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.affiliation: Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Fang
authors.affiliation: Institute of Cotton Research of Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology, Anyang 455000, Henan, China. Electronic address: zhoudy@cricaas.com.cn.
authors.forename: Dayun
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism*; D003368:Gossypium / Q000737:chemistry*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry; D011312:Pressure
chemical_list: D000468:Alkalies; D002241:Carbohydrates; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase
keywords: Cotton stalk; Enzymatic saccharification; High pressure assist-alkali pretreatment (HPAP); RSM; Structural characterization