title: Multiple detection of single nucleotide polymorphism by microarray-based resonance light scattering assay with enlarged gold nanoparticle probes.
abstract: The mapping of specific single nucleotide polymorphisms (SNPs) in patients' genome is a critical process for the development of personalized therapy. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of breast cancer related SNPs with high sensitivity and selectivity. After hybridization of the desired target single-stranded DNAs (ssDNAs) with the ssDNA probes on a microarray, the polyvalent ssDNA modified 13 nm gold nanoparticles (GNPs) are employed to label the hybridization reaction through the formation of a three-stranded DNA system. The H2O2-mediated enlargement of GNPs is then used to enhance the RLS signal. The microarray-based RLS assay provides a detection limit of 10 pM (S/N = 3) for the target ssDNA and determines an allele frequency as low as 1.0% in the target ssDNA cocktail. Combined with an asymmetric PCR technique, the proposed assay shows good accuracy and sensitivity in profiling 4 SNPs related to breast cancer of three selected cell lines.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. wangzx@ciac.ac.cn and University of Chinese Academy of Sciences, Beijing, 100039, P. R. China.
authors.forename: Jiaxue
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. wangzx@ciac.ac.cn and Analysis and Testing Center, Ningxia University, Yinchuan, 750021, P. R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. wangzx@ciac.ac.cn and University of Chinese Academy of Sciences, Beijing, 100039, P. R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. wangzx@ciac.ac.cn.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics*; D005240:Feasibility Studies; D005787:Gene Frequency; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008027:Light; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010316:Particle Size; D012542:Scattering, Radiation
chemical_list: D004277:DNA, Single-Stranded; D006046:Gold