title: Lignosulfonic Acid-Induced Inhibition of Intestinal Glucose Absorption.
abstract: Lignosulfonic acid is a waste lignin produced from the sulfite pulping of softwood. We investigated the effect of lignosulfonic acid on α-glucosidase and found that lignosulfonic acid produced a reversible and non-competitive inhibition of the enzyme activity. Moreover, in human colorectal adenocarcinoma cells, lignosulfonic acid inhibited 2-deoxyglucose uptake, while in vivo studies demonstrated a significant reduction in the blood glycemic response to sucrose or glucose ingestion in rats treated with lignosulfonic acid. Feces of rats fed a diet supplemented with 5% lignosulfonic acid had higher sugar content compared to those of rats fed a control diet. These results suggest that lignosulfonic acid suppresses the rise in blood glucose levels through inhibition of α-glucosidase activity and intestinal glucose absorption.
authors.affiliation: College of Environmental Technology, Muroran Institute of Technology.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Yukiya
authors.initials: Y
authors.lastname: Kadota
authors.forename: Chihiro
authors.initials: C
authors.lastname: Hasegawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawaminami
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D018938:Caco-2 Cells; D050260:Carbohydrate Metabolism / Q000187:drug effects; D045744:Cell Line, Tumor; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D019587:Dietary Supplements; D005243:Feces / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D065089:Glycoside Hydrolase Inhibitors / Q000494:pharmacology*; D006801:Humans; D007220:Industrial Waste; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D008031:Lignin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D017208:Rats, Wistar; D013451:Sulfonic Acids / Q000494:pharmacology*; D014934:Wood / Q000737:chemistry; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D065089:Glycoside Hydrolase Inhibitors; D007220:Industrial Waste; D010936:Plant Extracts; D013451:Sulfonic Acids; D008031:Lignin; D003847:Deoxyglucose; D000520:alpha-Glucosidases; D005947:Glucose