title: Miscanthus×giganteus xylooligosaccharides: Purification and fermentation.
abstract: A procedure was developed to recover xylooligosaccharides (XOS) from Miscanthus×giganteus (M×G) hydrolyzate. M×G hydrolyzate was prepared using autohydrolysis, and XOS rich fractions were acquired using activated carbon adsorption and stepwise ethanol elution. The combined XOS fractions were purified using a series of ion exchange resin treatments. The end product, M×G XOS, had 89.1% (w/w) total substituted oligosaccharides (TSOS) composed of arabinose, glucose, xylose and acetyl group. Bifidobacterium adolescentis and Bifidobacterium catenulatum (health promoting bacteria) were cultured in vitro on M×G XOS and a commercial XOS source, which was used as a comparison. B. adolescentis grew to a higher cell density than B. catenulatum in both XOS cultures. Total xylose consumption for B. adolescentis was 84.1 and 84.8%, respectively for M×G and commercial XOS cultures; and for B. catenulatum was 76.6 and 73.6%, respectively. The xylobiose (X2), xylotriose (X3) and xylotetraose (X4) were almost utilized for both strains. Acetic and lactic acids were the major fermentation products of the XOS cultures.
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA.
authors.forename: Ming-Hsu
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bowman
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cotta
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Dien
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Loren B
authors.initials: LB
authors.lastname: Iten
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Terence R
authors.initials: TR
authors.lastname: Whitehead
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA.
authors.forename: Kent D
authors.initials: KD
authors.lastname: Rausch
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Tumbleson
authors.affiliation: Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA. Electronic address: vsingh@illinois.edu.
authors.forename: Vijay
authors.initials: V
authors.lastname: Singh
mesh_terms: D001644:Bifidobacterium / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation; D005591:Chemical Fractionation / Q000379:methods*; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry; D005285:Fermentation; D005965:Glucuronates / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism*; D006868:Hydrolysis; D007475:Ion Exchange Resins / Q000737:chemistry; D009844:Oligosaccharides / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism*; D006109:Poaceae / Q000737:chemistry*; D014994:Xylose / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005965:Glucuronates; D007475:Ion Exchange Resins; D009844:Oligosaccharides; C570991:xylooligosaccharide; D014994:Xylose
keywords: Acetic acid; Acetic acid (PubChem CID: 176); Autohydrolysis; D-Glucose (PubChem CID: 5793); Fermentation; L-Arabinose (PubChem CID: 5460291); Lactic acid; Lactic acid (PubChem CID: 612); Miscanthus×giganteus; Xylobiose (PubChem CID: 439538); Xylohexaose (PubChem CID: 74539951); Xylooligosaccharides; Xylopentaose (PubChem CID: 10146542); Xylotetraose (PubChem CID: 10230811); Xylotriose (PubChem CID: 10201852); d-Xylose (PubChem CID: 135191)