title: In vitro alteration of hematological parameters and blood viscosity by the perfluorocarbon: Oxycyte.
abstract: While perfluorocarbons (PFCs) may be useful in some clinical situations, previous studies have shown that interferences with chemistry analytes can occur with blood samples containing PFCs. This in vitro study focused on how the PFC Oxycyte may affect hematology measurements in blood samples. Swine blood diluted with Oxycyte or saline (Controls) were analyzed for Hemoglobin (Hb), Mean Corpuscular Volume (MCV),Hematocrit (Hct) and Fluorocrit (Fct) using a HemaVet, ABL-735 (ABL), or microhematocrit. Ancillary tests (blood viscosity, electrolytes, cell counts, and red blood cell morphology) were performed secondarily. Increasing Oxycyte resulted in increases in MCV, Hct, and visible cell shape change and morphology vs.
  
  
  METHODS
  Effects correlated with lower sodium in Oxycyte samples vs.
  
  
  METHODS
  With increasing Oxycyte, Hb became higher than Controls or became unpredictable depending on the instrument (HemaVet or ABL, respectively). Fct was smaller than predicted and likely represented the heaviest components of Oxycyte. At ≥50 % Oxycyte, RBC hemolysis rendered further measurements impractical. Viscosity first increased then decreased with increasing Oxycyte, peaking at ~40 % Oxycyte. Hct, MCV, Hb, and RBC morphology may be affected by Oxycyte. These observations correlated with lower sodium and increasing Oxycyte, causing hemolysis at high Oxycyte concentrations. These changes were due to alterations in the blood samples in vitro and this should be considered when interpreting hematology parameters from in vivo studies.
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA. francoise.arnaud.ctr@mail.mil.|Department of Surgery, USUHS, National Naval Medical Center, Bethesda, MD, 20889, USA. francoise.arnaud.ctr@mail.mil.
authors.forename: Françoise
authors.initials: F
authors.lastname: Arnaud
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Sanders
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
authors.forename: Donna
authors.initials: D
authors.lastname: Sieckmann
authors.affiliation: NeuroTrauma Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Moon-Massat
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D001809:Blood Viscosity / Q000187:drug effects*; D004909:Erythrocyte Indices / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005466:Fluorocarbons / Q000494:pharmacology*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis / Q000187:drug effects; D013552:Swine
chemical_list: D001802:Blood Substitutes; D005466:Fluorocarbons; D006454:Hemoglobins
keywords: Diagnostics; Emulsion; Oxygen therapy; Perfluorocarbon