title: Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells.
abstract: Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian serous cystadenocarcinoma (OV) dataset demonstrated the overexpression of TMEM14A in ovarian cancer tissues compared with normal tissues, which was consistent with our real-time PCR analysis on ovarian cancer and normal tissues collected from 30 patients. In addition, TMEM14A knockdown in two ovarian cancer cell lines, A2780 and HO-8910, reduced cell proliferation, causes cell cycle arrest and suppressed cell invasion. Moreover, silencing of TMEM14A notably repressed G1/S cell cycle transition and cell invasion via down-regulating the expression of cell cycle related proteins (Cyclin D1, Cyclin E and PCNA) and metastasis-related proteins (MMP-2 and MMP-9), respectively. TMEM14A knockdown significantly reduced the phosphorylation status of Smad2 and Smad3, downstream effectors of TGF-β signalling. In summary, these results indicate that TMEM14A has a pro-tumorigenic effect in ovarian cancer cells, suggesting an important role of this protein in ovarian cancer oncogenesis and metastasis.
authors.affiliation: FuJian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Longyan Hospital of Traditional Chinese Medicine, Longyan 364000, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Gynecology, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, No. 602 of 817 Middle Road, Fuzhou 350004, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Gynecology, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, No. 602 of 817 Middle Road, Fuzhou 350004, China.
authors.forename: Jingfen
authors.initials: J
authors.lastname: Zhan
authors.affiliation: Department of Gynecology, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, No. 602 of 817 Middle Road, Fuzhou 350004, China jchenfz@sina.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C561462:TMEM14A protein, human
keywords: TMEM14A; cell cycle; metastasis; ovarian cancer