title: Inhibitory effects of compounds isolated from the dried branches and leaves of murta (Myrceugenia euosma) on lipid accumulation in 3T3-L1 cells.
abstract: As obesity is a global health concern the demand for anti-obesity drugs is high. In this study, we investigated the anti-obesity effect of the dried branches and leaves of murta (Myrceugenia euosma Legrand, Myrtaceae). A methanol extract of the dried branches and leaves of murta inhibited adipogenesis in 3T3-L1 cells. Three known flavanones-cryptostrobin (1), pinocembrin (4), and 5,7-dihydroxy-6,8-dimethylflavanone (6), and three chalcones-2',6'-dihydroxy-3'-methyl-4'-methoxychalcone (2), pinostrobin chalcone (3), and 2',6'-dihydroxy-4'-methoxy-3',5'-dimethylchalcone (5) were isolated from the active fraction. Structures of these compounds were identified using various spectral data. Each of these compounds also inhibited adipogenesis in 3T3-L1 cells. In particular, compound 3 was a more potent inhibitor of triglyceride accumulation than the positive control berberine. Gene expression studies revealed that treatment of 3T3-L1 cells with 3 lowers the expression levels of CCAAT/enhancer-binding protein α and peroxisome proliferator activator γ2 during adipogenesis without affecting cell viability. Treatment of 3T3-L1 cells with 3 reduced the expression levels of mRNAs encoding sterol regulatory element-binding protein 1c and several lipogenic enzymes, including fatty acid synthase and stearoyl CoA desaturase-1. These results indicate that the methanol extract and compounds isolated from the dried branches and leaves of murta exert their anti-obesity effects through the inhibition of adipogenesis.
authors.affiliation: School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba, 274-8555, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Oikawa
authors.affiliation: School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba, 274-8555, Japan. nobushi.yasuhito@nihon-u.ac.jp.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Nobushi
authors.affiliation: School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba, 274-8555, Japan.
authors.forename: Taira
authors.initials: T
authors.lastname: Wada
authors.affiliation: School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba, 274-8555, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sonoda
authors.affiliation: Amazonfood Ltd., 3-1-8, Misaki, Chiyoda, Tokyo, 101-0061, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Okazaki
authors.affiliation: Amazonfood Ltd., 3-1-8, Misaki, Chiyoda, Tokyo, 101-0061, Japan.
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Tsutsumi
authors.affiliation: Department of Food Science, College of Food Engineering, State University of Campinas, Campinas, SP, Brazil.
authors.forename: Yong Kun
authors.initials: YK
authors.lastname: Park
authors.affiliation: Department of Biochemistry, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki, 882-8508, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurokawa
authors.affiliation: School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba, 274-8555, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Shimba
authors.affiliation: School of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi, Chiba, 274-8555, Japan. yasukawa.ken@nihon-u.ac.jp.
authors.forename: Ken
authors.initials: K
authors.lastname: Yasukawa
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D000068856:Eugenia / Q000737:chemistry*; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D019440:Anti-Obesity Agents; D010936:Plant Extracts
keywords: 3T3-L1 cells; Lipid accumulation; Metabolic syndrome; Murta; Obesity