title: Anaerobic digestion of biowaste under extreme ammonia concentration: Identification of key microbial phylotypes.
abstract: Ammonia inhibition represents a major operational issue for anaerobic digestion (AD). In order to get more insights into AD microbiota resistance, anaerobic batch reactors performances were investigated under a wide range of Total Ammonia Nitrogen (TAN) concentrations up to 50.0g/L at 35°C. The half maximal inhibitory concentration (IC50) value was determined to be 19.0g/L. Microbial community dynamics revealed that above a TAN concentration of 10.0g/L, remarkable modifications within archaeal and bacterial communities occurred. 16S rRNA gene sequencing analysis showed a gradual methanogenic shift between two OTUs from genus Methanosarcina when TAN concentration increased up to 25.0g/L. Proportion of potential syntrophic microorganisms such as Methanoculleus and Treponema progressively raised with increasing TAN up to 10.0 and 25.0g/L respectively, while Syntrophomonas and Ruminococcus groups declined. In 25.0g/L assays, Caldicoprobacter were dominant. This study highlights the emergence of AD key phylotypes at extreme ammonia concentrations.
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France. Electronic address: simon.poirier@irstea.fr.
authors.forename: Simon
authors.initials: S
authors.lastname: Poirier
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France.
authors.forename: Elie
authors.initials: E
authors.lastname: Desmond-Le Quéméner
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Madigou
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France.
authors.forename: Théodore
authors.initials: T
authors.lastname: Bouchez
authors.affiliation: Hydrosystems and Bioprocesses Research Unit, Irstea, 1 rue Pierre-Gilles de Gennes, CS 10030, 92761 Antony Cedex, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Chapleur
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000235:genetics*; D019149:Bioreactors / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008697:Methane / Q000737:chemistry*; D019638:Methanomicrobiaceae / Q000235:genetics; D017020:Methanosarcina / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045857:Ruminococcus / Q000235:genetics; D012984:Software; D013696:Temperature; D014208:Treponema / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D008697:Methane
keywords: 16S rRNA gene sequencing; ARISA; Ammonia; IC(50); Principal Component Analysis