title: Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice.
abstract: Stem cells are cells capable of proliferation, self-renewal, and differentiation into specific phenotypes. They are an essential part of tissue engineering, which is used in regenerative medicine in case of degenerative diseases. In this chapter, we describe the methods of isolating and culturing various types of stem cells, like human embryonic stem cells (hESCs), human umbilical cord derived mesenchymal stem cells (hUC-MSCs), murine bone marrow derived mesenchymal stem cells (mBM-MSCs), murine adipose tissue derived mesenchymal stem cells (mAD-MSCs), and murine bone marrow derived dendritic cells (mBMDCs). All these cell types can be used in tissue engineering techniques.
authors.affiliation: Immunology and Regenerative Medicine Research Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Srabani
authors.initials: S
authors.lastname: Kar
authors.affiliation: Immunology and Regenerative Medicine Research Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
authors.forename: Shinjini
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Immunology and Regenerative Medicine Research Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India. enarb1@gmail.com.|Department of Zoology, University of Calcutta, Kolkata, 700019, West Bengal, India. enarb1@gmail.com.
authors.forename: Ena Ray
authors.initials: ER
authors.lastname: Banerjee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002469:Cell Separation / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology
keywords: Adipose derived mesenchymal stem cells (AD-MSCs); Bone marrow derived dendritic cells (BMDCs); Bone marrow derived mesenchymal stem cells (BM-MSCs); Embryoid body (EB); Embryonic stem cells (ESCs); Lineage specific differentiation; Mesenchymal stem cells (MSCs); Stem cells; Umbilical cord (UC); Undifferentiated cells