title: A genome-wide resource for the analysis of protein localisation in Drosophila.
abstract: The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Mihail
authors.identifier: http://orcid.org/0000-0003-2895-4087
authors.initials: M
authors.lastname: Sarov
authors.affiliation: Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Barz
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Helena
authors.initials: H
authors.lastname: Jambor
authors.affiliation: Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Marco Y
authors.identifier: http://orcid.org/0000-0002-9490-2261
authors.initials: MY
authors.lastname: Hein
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Christopher
authors.initials: C
authors.lastname: Schmied
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Dana
authors.initials: D
authors.lastname: Suchold
authors.affiliation: Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Stender
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Janosch
authors.affiliation: Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
authors.forename: Vinay Vikas
authors.identifier: http://orcid.org/0000-0003-1328-9395
authors.initials: VV
authors.lastname: K J
authors.affiliation: Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
authors.forename: R T
authors.initials: RT
authors.lastname: Krishnan
authors.affiliation: Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
authors.forename: Aishwarya
authors.identifier: http://orcid.org/0000-0001-8245-3828
authors.initials: A
authors.lastname: Krishnamoorthy
authors.affiliation: Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Irene R S
authors.initials: IR
authors.lastname: Ferreira
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Radoslaw K
authors.initials: RK
authors.lastname: Ejsmont
authors.affiliation: Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Katja
authors.initials: K
authors.lastname: Finkl
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Hasse
authors.affiliation: Heidelberg Institute of Theoretical Studies, Heidelberg, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Plewka
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Vinis
authors.affiliation: Heidelberg Institute of Theoretical Studies, Heidelberg, Germany.
authors.forename: Siegfried
authors.initials: S
authors.lastname: Schloissnig
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Knust
authors.affiliation: Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
authors.forename: Volker
authors.initials: V
authors.lastname: Hartenstein
authors.affiliation: Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
authors.affiliation: Institute of Neuroscience, Trinity College Dublin, Dublin, Ireland.
authors.forename: Mani
authors.initials: M
authors.lastname: Ramaswami
authors.affiliation: Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.
authors.forename: K
authors.identifier: http://orcid.org/0000-0002-4705-5629
authors.initials: K
authors.lastname: VijayRaghavan
authors.affiliation: Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
authors.forename: Pavel
authors.identifier: http://orcid.org/0000-0002-2222-9370
authors.initials: P
authors.lastname: Tomancak
authors.affiliation: Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Frank
authors.identifier: http://orcid.org/0000-0002-9518-7263
authors.initials: F
authors.lastname: Schnorrer
mesh_terms: D000825:Animal Structures / Q000737:chemistry; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D004330:Drosophila / Q000737:chemistry* / Q000235:genetics*; D029721:Drosophila Proteins / Q000032:analysis* / Q000235:genetics*; D004772:Entomology / Q000379:methods; D015723:Gene Library; D017930:Genes, Reporter; D049750:Genome, Insect; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D008967:Molecular Biology / Q000379:methods; D061848:Optical Imaging; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D029721:Drosophila Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
keywords: <i>d. melanogaster</i>; developmental biology; drosophila; evolutionary biology; genetics; genomics; live imaging; protein tagging; recombineering; resource; stem cells