title: Nocardioides ginkgobilobae sp. nov., an endophytic actinobacterium isolated from the root of the living fossil Ginkgo biloba L.
abstract: A Gram-stain-positive, aerobic and yellow actinobacterial strain, designated SYP-A7303T, was isolated from the root of Ginkgo biloba L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-A7303T belongs to the genus Nocardioides. The 16S rRNA gene sequence of strain SYP-A7303T showed highest similarity to Nocardioides marinus CL-DD14T ( = JCM 15615T) (98.3 %) and Nocardioides aquiterrae GW-9T ( = JCM 11813T) (97.1 %), and less than 96.9 % to the type strains of other species of the genus Nocardioides. Strain SYP-A7303T grew optimally at 28 °C, pH 7.0 and in the absence of NaCl. It contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, with mannose, ribose, rhamnose, glucose and galactose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown lipid. The menaquinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The DNA G+C content was 72 mol%. Mean DNA-DNA relatedness values between strain SYP-A7303T and the closely related strains N. marinus JCM 15615T and N. aquiterrae JCM 11813T were 62.5 ± 2.4 and 56.5 ± 3.5 %, respectively. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain SYP-A7303T represents a novel species of the genus Nocardioides, for which the name Nocardioides ginkgobilobae sp. nov. is proposed. The type strain is SYP-A7303T ( = DSM 100492T = KCTC 39594T).
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang,110016, PRChina.
authors.forename: Huan
authors.initials: H
authors.lastname: Xu
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang,110016, PRChina.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang,110016, PRChina.
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275, PRChina.
authors.forename: Mipeshwaree Devi
authors.initials: MD
authors.lastname: Asem
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang,110016, PRChina.
authors.forename: Meng-Yue
authors.initials: MY
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275, PRChina.|Department of Microbiology, Gulbarga University,Kalaburgi, 585106,India.
authors.forename: Deene
authors.initials: D
authors.lastname: Manikprabhu
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang,110016, PRChina.
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Zhang
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang,110016, PRChina.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University,Guangzhou, 510275, PRChina.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang,110016, PRChina.
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D020441:Ginkgo biloba / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid