title: Roseomonas chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from activated sludge.
abstract: A Gram-negative, aerobic, short rod-shaped, pink-pigmented, non-motile bacterium, designated BUT-13(T), was isolated from activated sludge of an herbicide-manufacturing wastewater treatment facility in Jiangsu province, China. Growth was observed at 0-5.5 % NaCl, pH 6.0-9.0 and 12-37 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BUT-13(T) is a member of the genus Roseomonas, and shows high sequence similarities to R. pecuniae N75(T) (98.0 %) and R. rosea 173-96(T) (97.5 %), and lower (<97 %) sequence similarities to all other Roseomonas species. Chemotaxonomic analysis revealed that strain BUT-13(T) possesses Q-10 as the predominant ubiquinone; summed feature 8 (C18:1 w7c and/or C18:1 w6c; 38.8 %), C18:0 (16.6 %), C16:0 (15.2 %), summed feature 3 (C16:1 ω6c and/or C16:1 ω7; 7.9 %) and C18:1 w9c (4.7 %) as the major fatty acids. The polar lipids were found to consist of two aminolipids, a glycolipid, a phospholipid, a phosphoglycolipid, phosphatidylcholine, phosphatidylethanolamine and diphosphatidylglycerol. Strain BUT-13(T) showed low DNA-DNA relatedness with R. pecuniae N75(T) (45.2 %) and R. rosea 173-96(T) (51.2 %). The DNA G+C content was determined to be 67.6 mol%. Based on the phylogenetic analysis, DNA-DNA hybridization and chemotaxonomic analysis, as well as biochemical characteristics, strain BUT-13(T) can be clearly distinguished from all currently recognised Roseomonas species and should be classified as a novel species of the genus Roseomonas, for which the name Roseomonas chloroacetimidivorans sp. nov. is proposed. The type strain is BUT-13(T) (CCTCC AB 2015299(T) = JCM 31050(T)).
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
authors.forename: Cui-Wei
authors.initials: CW
authors.lastname: Chu
authors.affiliation: College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, Shandong, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory of Systems Biology Medicine of Jiangxi Province, College of Basic Medical Science, Jiujiang University, Jiujiang, 332000, Jiangxi, People's Republic of China.
authors.forename: Cheng-Hong
authors.initials: CH
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, Shandong, People's Republic of China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, Shandong, People's Republic of China.
authors.forename: Zhong-Guan
authors.initials: ZG
authors.lastname: Sun
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: He
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China. hejian@njau.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Agricultural Culture Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. jggu@caas.ac.cn.
authors.forename: Jin-Gang
authors.initials: JG
authors.lastname: Gu
mesh_terms: D000081:Acetamides / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D006540:Herbicides / Q000378:metabolism*; D000072499:Manufacturing and Industrial Facilities; D042082:Methylobacteriaceae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D012988:Soil Microbiology; D062065:Wastewater / Q000382:microbiology
chemical_list: D000081:Acetamides; D004269:DNA, Bacterial; D006540:Herbicides; D012722:Sewage; D062065:Wastewater; C013874:chloroacetamide
keywords: Degrading; Polyphasic taxonomy; Roseomonas chloroacetimidivorans sp. nov