title: 'Does my Diet Affect my Perfume?' Identification and Quantification of Cuticular Compounds in Five Drosophila melanogaster Strains Maintained over 300 Generations on Different Diets.
abstract: Cuticular hydrocarbons (CHCs) in Drosophila melanogaster represent the basis of chemical communication being involved in many important biological functions. The aim of this study was to characterize chemical composition and variation of cuticular profiles in five D. melanogaster strains. These strains were reared for approximately 300 generations on five diets: standard cornmeal medium and substrates prepared with apple, banana, tomato, and carrot. Differences in quantity and/or quality in CHCs were assumed as a result of activation of different metabolic pathways involved in food digestion and adaptations to the particular diet type. In total, independently of sex and strain, 66 chemical compounds were identified. In females of all strains, 60 compounds were identified, while, in males, 47 compounds were extracted. Certain new chemical compounds for D. melanogaster were found. MANOVA confirmed that CHC amounts significantly depend on sex and substrates, as well as on their interactions. Discriminant analysis revealed that flies belonging to 'apple' and 'carrot' strains exhibited the most noticeable differences in CHC repertoires. A non-hydrocarbon pheromone, cis-vaccenyl acetate (cVA) also contributed to the variation in the pheromone bouquet among the strains. Variability detected in CHCs and cVA may be used in the explanation of differences in mating behaviour previously determined in analyzed fly strains.
authors.affiliation: University of Belgrade, Faculty of Biology, Studentski trg 16, RS-11000 Belgrade.
authors.forename: Sofija
authors.initials: S
authors.lastname: Pavković-Lučić
authors.affiliation: University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, RS-11000 Belgrade.
authors.forename: Marina
authors.initials: M
authors.lastname: Todosijević
authors.affiliation: University of Belgrade, Institute for Biological Research 'Siniša Stanković', Despota Stefana Blvd. 142, RS-11000 Belgrade.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Savić
authors.affiliation: University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, RS-11000 Belgrade.
authors.forename: Vlatka
authors.initials: V
authors.lastname: Vajs
authors.affiliation: University of Belgrade, Institute for Biological Research 'Siniša Stanković', Despota Stefana Blvd. 142, RS-11000 Belgrade.
authors.forename: Jelena
authors.initials: J
authors.lastname: Trajković
authors.affiliation: University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, RS-11000 Belgrade.
authors.forename: Boban
authors.initials: B
authors.lastname: Anđelković
authors.affiliation: University of Belgrade, Faculty of Biology, Studentski trg 16, RS-11000 Belgrade.
authors.forename: Luka
authors.initials: L
authors.lastname: Lučić
authors.affiliation: University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, RS-11000 Belgrade.
authors.forename: Gordana
authors.initials: G
authors.lastname: Krstić
authors.affiliation: University of Belgrade, Faculty of Biology, Studentski trg 16, RS-11000 Belgrade.
authors.forename: Slobodan
authors.initials: S
authors.lastname: Makarov
authors.affiliation: University of Belgrade, Faculty of Biology, Studentski trg 16, RS-11000 Belgrade.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Tomić
authors.affiliation: University of Belgrade, Faculty of Biology, Studentski trg 16, RS-11000 Belgrade.
authors.forename: Dragana
authors.initials: D
authors.lastname: Miličić
authors.affiliation: University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, RS-11000 Belgrade.
authors.forename: Ljubodrag
authors.initials: L
authors.lastname: Vujisić
mesh_terms: D000085:Acetates / Q000032:analysis / Q000378:metabolism; D000818:Animals; D004032:Diet; D004331:Drosophila melanogaster / Q000737:chemistry / Q000502:physiology*; D005260:Female; D006838:Hydrocarbons / Q000032:analysis* / Q000378:metabolism; D008297:Male; D009829:Oleic Acids / Q000032:analysis / Q000378:metabolism; D010675:Pheromones / Q000032:analysis* / Q000378:metabolism
chemical_list: D000085:Acetates; D006838:Hydrocarbons; D009829:Oleic Acids; D010675:Pheromones; C505385:cis-vaccenyl acetate
keywords: Cuticular hydrocarbons; Diet; Drosophila melanogaster; cis-Vaccenyl acetate