title: Rapid multiplex detection of 10 foodborne pathogens with an up-converting phosphor technology-based 10-channel lateral flow assay.
abstract: The rapid high-throughput detection of foodborne pathogens is essential in controlling food safety. In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was established for the rapid and simultaneous detection of 10 epidemic foodborne pathogens. Ten different single-target UPT-LF strips were developed and integrated into one TC-UPT-LF disc with optimization. Without enrichment the TC-UPT-LF assay had a detection sensitivity of 10(4) CFU mL(-1) or 10(5) CFU mL(-1) for each pathogen, and after sample enrichment it was 10 CFU/0.6 mg. The assay also showed good linearity, allowing quantitative detection, with a linear fitting coefficient of determination (R(2)) of 0.916-0.998. The 10 detection channels did not cross-react, so multiple targets could be specifically detected. When 279 real food samples were tested, the assay was highly consistent (100%) with culture-based methods. The results for 110 food samples artificially contaminated with single or multiple targets showed a high detection rate (≥ 80%) for most target bacteria. Overall, the TC-UPT-LF assay allows the rapid, quantitative, and simultaneous detection of 10 kinds of foodborne pathogens within 20 min, and is especially suitable for the rapid detection and surveillance of foodborne pathogens in food and water.
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Food and Nutrition, Massey University, Palmerston North 4442, New Zealand.|Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Wang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.|Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China.
authors.forename: Chongyun
authors.initials: C
authors.lastname: Sun
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.|College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, P. R. China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.|Institute for Plague Prevention and Control of Hebei Province, Zhangjiakou 075000, P. R. China.
authors.forename: Xinrui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Clinical Laboratory, Chinese People's Liberation Army General Hospital, Beijing 100853, P. R. China.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Wang
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China.|Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing 100071, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001681:Biological Assay; D015374:Biosensing Techniques / Q000379:methods / Q000592:standards; D004195:Disease Models, Animal; D005260:Female; D005516:Food Microbiology / Q000379:methods / Q000592:standards; D005517:Foodborne Diseases / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D051379:Mice; D008828:Microbiological Techniques / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity