title: Measurement of Resting Energy Metabolism in Mice Using Oxymax Open Circuit Indirect Calorimeter.
abstract: Indirect calorimeter is a powerful tool to monitor resting energy metabolism through the measurement of oxygen (O2) consumption and carbon dioxide (CO2) production. From the measurement of VO2and VCO2, the respiratory exchange ratio (RER) can be calculated to assess energy fuel utilization and energy expenditure (Evanet al., 2012). Previously, indirect calorimeter has been widely used in metabolic disease research in mice to reveal the potential roles of specific genes or treatments in regulating energy metabolism (for example: Biet al., 2014; Fenget al., 2014). Here, we described a protocol to evaluate the resting energy metabolism of C57BL/6 mice during dark and light cycles using the Oxymax Open Circuit indirect calorimeter.
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, USA; Department of Health and Kinesiology and Max E. Wastl Human Performance Laboratory, Purdue University, West Lafayette, USA.
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Department of Health and Kinesiology and Max E. Wastl Human Performance Laboratory, Purdue University, West Lafayette, USA.
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Gavin
authors.affiliation: Department of Animal Sciences, Purdue University, West Lafayette, USA; Center for Cancer Research, Purdue University, West Lafayette, USA.
authors.forename: Shihuan
authors.initials: S
authors.lastname: Kuang