title: Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China.
abstract: OBJECTIVE
  There are a lot of disagreements in the studies on hepatitis B virus (HBV) DNA polymerase mutation rate associated with nucleos(t)ide analogues (NAs) in treatment-naive chronic hepatitis B (CHB) patients. This is the first study aimed to investigate the prevalence of spontaneous HBV resistance mutations in Central China.
  
  
  METHODS
  This study included treatment-naive patients with CHB from June 2012 to May 2015 receiving care at the Institute of Liver Disease in Central China. All patients completed a questionnaire covering different aspects, such as family medical history, course of liver disease, medication history, alcohol use, among others. Mutations in HBV DNA polymerase associated with NAs resistance were detected using INNO-LiPA assay.
  
  
  RESULTS
  269 patients were infected with HBV genotype B (81.4%), C (17.9%), and both B and C (0.7%). Mutations in HBV DNA polymerase were detected in 24 patients (8.9%) including rtM204I/V (n=6), rtN236T (n=5), rtM250V (n=2), rtL180M (n=2), rtT184G (n=1), rtM207I (n=1), rtS202I (n=1), rtM204V/I & rtL180M (n=5), and rtM204I & rtM250V (n=1).
  
  
  CONCLUSIONS
  Spontaneous HBV resistance mutations in HBV DNA polymerase were found in treatment-naive patients with CHB in Central China. These findings suggest that we should analyze HBV DNA polymerase resistance mutation associated with NAs before giving antiviral therapy such as lamivudine (LAM), adefovir (ADV), and telbivudine (LdT).
authors.affiliation: Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China; Department of Clinical Laboratory, Hubei University of Traditional Chinese Medicine, Wuhan, China. Electronic address: zhaoyy206@163.com.
authors.forename: Youyun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China. Electronic address: hjw1959@vip.sina.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wu
authors.affiliation: Hubei Center for Clinical Laboratory, Wuhan 430064, China. Electronic address: Sun8882005@163.com.
authors.forename: Lijun
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
authors.forename: Guangzhong
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Institute of Liver Disease, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Liver Disease, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
authors.forename: Junxiu
authors.initials: J
authors.lastname: Tao
mesh_terms: D000328:Adult; D000368:Aged; D000998:Antiviral Agents / Q000627:therapeutic use; D002681:China; D004279:DNA, Viral / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D024882:Drug Resistance, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D011247:Pregnancy; D015995:Prevalence; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase
keywords: Chronic hepatitis B; Hepatitis B virus; Viral resistance mutations