title: Possible involvement of miRNAs in tropism of Parvovirus B19.
abstract: Human Parvovirus B19 (PVB19) is one of the most important pathogens that targets erythroid lineage. Many factors were mentioned for restriction to erythroid progenitor cells (EPCs). Previous studies showed that in non-permissive cells VP1 and VP2 (structural proteins) mRNAs were detected but could not translate to proteins. A bioinformatics study showed that this inhibition might be due to specific microRNAs (miRNAs) present in non-permissive cells but not in permissive EPCs. To confirm the hypothesis, we evaluated the effect of miRNAs on VP expression. CD34(+) HSCs were separated from cord blood. Then, CD34(+) cells were treated with differentiation medium to obtain CD36(+) EPCs. To evaluate the effect of miRNAs on VP expression in MCF7 and HEK-293 cell lines (non-permissive cells) and CD36(+) EPCs, dual luciferase assay was performed in presence of shRNAs against Dicer and Drosha to disrupt miRNA biogenesis. QRT-PCR was performed to check down-regulation of Dicer and Drosha after transfection. All measurements were done in triplicate. Data means were compared using one-way ANOVAs. MicroRNA prediction was done by the online microRNA prediction tools. No significant difference was shown in luciferase activity of CD36(+) EPCs after co-transfection with shRNAs, while it was significant in non-permissive cells. Our study revealed that miRNAs may be involved in inhibition of VP expression in non-permissive cells, although further studies are required to demonstrate which miRNAs exactly are involved in regulation of PVB19 replication.
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Anbarlou
authors.affiliation: Department of Hematology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mahshid
authors.initials: M
authors.lastname: AkhavanRahnama
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Iran. atashia@gmail.com.
authors.forename: Amir
authors.initials: A
authors.lastname: Atashi
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box 14115-331, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Arefian
authors.affiliation: Department of Pharmacy and Biotechnology, S.Orsola-Malpighi Hospital - Microbiology, University of Bologna, Bologna, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Gallinella
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D019295:Computational Biology; D015672:Erythroid Precursor Cells / Q000378:metabolism* / Q000821:virology; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs; D010322:Parvoviridae Infections / Q000235:genetics / Q000378:metabolism*; D016732:Parvovirus B19, Human / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger; D018523:Tropism
chemical_list: D036022:Capsid Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; C095547:capsid protein VP1, parvovirus B19; C095548:capsid protein VP2, parvovirus B19
keywords: Erythroid Progenitors; HEK-293; MCF7; Parvovirus B19; microRNAs