title: Characterization of an Apis cerana cerana cytochrome P450 gene (AccCYP336A1) and its roles in oxidative stresses responses.
abstract: Cytochrome P450 monooxygenases (P450), widely distributed multifunctional enzymes, that play an important role in the oxidative metabolism of endogenous compounds and xenobiotics. Studies have found that these enzymes show peroxidase-like activity and may thus be involved in protecting organisms against reactive oxygen species (ROS). In this work, Apis cerana cerana was used to investigate the molecular mechanisms of P450 family genes in resisting ROS damage. A cytochrome P450 gene was isolated, AccCYP336A1. The open reading frame (ORF) of AccCYP336A1 is 1491bp in length and encodes a predicted protein of 496 amino acids. The obtained amino acid sequence of AccCYP336A1 shared a high sequence identity with homologous proteins and contained the highly conserved features of this protein family. Quantitative real-time PCR (qRT-PCR) analysis showed that AccCYP336A1 was present in some fast developmental stages and had a higher expression in the epidermis than in other tissues. Additionally, the expression levels of AccCYP336A1 were up-regulated by cold (4 °C), heat (42 °C), ultraviolet (UV) radiation, H2O2 and pesticide (thiamethoxam, deltamethrin, methomyl and phoxim) treatments. These results were confirmed by the western blot assays. Furthermore, the recombinant AccCYP336A1 protein acted as an antioxidant that resisted paraquat-induced oxidative stress. Taken together, these results suggest that AccCYP336A1 may play a very significant role in antioxidant defense against ROS damage.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Weixing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: lihan@sdau.edu.cn.
authors.forename: Han
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: bhxu@sdau.edu.cn.
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000145:classification / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000235:genetics*; D010802:Phylogeny; D017382:Reactive Oxygen Species / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D017382:Reactive Oxygen Species; D003577:Cytochrome P-450 Enzyme System
keywords: Antioxidant defense; Apis cerana cerana; Cytochrome P450 monooxygenases; Reactive oxidative stress