title: Cellulose-Based Biosensors for Esterase Detection.
abstract: Cellulose has emerged as an attractive substrate for the production of economical, disposable, point-of-care (POC) analytical devices. Development of novel methods of (bio)activation is central to broadening the application space of cellulosic materials. Ironically, such efforts are stymied by the inherent biocompatibility and recalcitrance of cellulose fibers. Here, we have elaborated a versatile, chemo-enzymatic approach to activate cellulosic materials for CuAAC "click chemistry", to develop new fluorogenic esterase sensors. Gentle, aqueous modification conditions facilitate broad applicability to cellulose papers, gauzes, and hydrogels. Tethering of the released fluorophore to the cellulose surface prevents signal degradation due to diffusion and enables straightforward, sensitive visualization with a simple light source in resource-limited situations.
authors.affiliation: The Michael Smith Laboratories and Department of Chemistry, University of British Columbia , 2185 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Derikvand
authors.affiliation: The Michael Smith Laboratories and Department of Chemistry, University of British Columbia , 2185 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: DeLu Tyler
authors.initials: DT
authors.lastname: Yin
authors.affiliation: The Michael Smith Laboratories and Department of Chemistry, University of British Columbia , 2185 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: Ryan
authors.initials: R
authors.lastname: Barrett
authors.affiliation: The Michael Smith Laboratories and Department of Chemistry, University of British Columbia , 2185 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: Harry
authors.initials: H
authors.lastname: Brumer
mesh_terms: D015374:Biosensing Techniques; D002482:Cellulose / Q000737:chemistry*; D004950:Esterases / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D002482:Cellulose; D004950:Esterases