title: Hydroxyethyl starch solution for extracorporeal tissue perfusion.
abstract: In the field of free flap transfer in reconstructive surgery, the trans- or replanted tissue always undergoes cell damage during ischemia to a more or less strong extent. In previous studies we already showed that conserving muscle transplants by means of extracorporeal perfusion over a period of 6 hours by using a crystalloid solution for perfusion. However, we observed significant edema formation. In this study we aimed at reducing the edema formation by using an iso-oncotic colloid as perfusion solution. This way we wanted to evaluate a possible new application of hydroxyl-ethyl starch in an extracorporeal setup to exploit potential benefits of the colloid.Examined parameters include the muscles' functionality with external field stimulation, histological examination and edema formation. Perfused muscles showed a statistically significant higher ability to exert force compared to nonperfused ones. These findings can be confirmed using Annexin V as marker for cell damage, as perfusion of muscle tissue limits damage significantly compared to nonperfused tissue. Substituting the electrolyte perfusion solution with a colloidal one shows the tendency to reduce the edema formation however without statistical significance.
authors.affiliation: Department of Plastic and Hand Surgery, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (FAU), Erlangen, Germany.
authors.forename: Christian D
authors.initials: CD
authors.lastname: Taeger
authors.affiliation: Institute of Medical Biotechnology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Friedrich
authors.affiliation: Department of Plastic and Hand Surgery, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (FAU), Erlangen, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Drechsler
authors.affiliation: Department of Plastic and Hand Surgery, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (FAU), Erlangen, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Weigand
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
authors.forename: Frieder
authors.initials: F
authors.lastname: Hobe
authors.affiliation: Chair of Pathology and Anatomy, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
authors.forename: Carol I
authors.initials: CI
authors.lastname: Geppert
authors.affiliation: Department of Paediatric Cardiac Surgery, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Münch
authors.affiliation: Department of Anesthesiology, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Birkholz
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Buchholz
authors.affiliation: Department of Plastic and Hand Surgery, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (FAU), Erlangen, Germany.
authors.forename: Raymund E
authors.initials: RE
authors.lastname: Horch
authors.affiliation: Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Präbst
mesh_terms: D000818:Animals; D000077324:Crystalloid Solutions; D006895:Hydroxyethyl Starch Derivatives / Q000627:therapeutic use*; D007511:Ischemia / Q000473:pathology*; D007552:Isotonic Solutions / Q000627:therapeutic use*; D010477:Perfusion / Q000379:methods*; D013552:Swine
chemical_list: D000077324:Crystalloid Solutions; D006895:Hydroxyethyl Starch Derivatives; D007552:Isotonic Solutions
keywords: Extracorporeal perfusion; free flap; hydroxyethyl starch; ischemia; transplantation