title: Microfluidic model of the platelet-generating organ: beyond bone marrow biomimetics.
abstract: We present a new, rapid method for producing blood platelets in vitro from cultured megakaryocytes based on a microfluidic device. This device consists in a wide array of VWF-coated micropillars. Such pillars act as anchors on megakaryocytes, allowing them to remain trapped in the device and subjected to hydrodynamic shear. The combined effect of anchoring and shear induces the elongation of megakaryocytes and finally their rupture into platelets and proplatelets. This process was observed with megakaryocytes from different origins and found to be robust. This original bioreactor design allows to process megakaryocytes at high throughput (millions per hour). Since platelets are produced in such a large amount, their extensive biological characterisation is possible and shows that platelets produced in this bioreactor are functional.
authors.affiliation: PlatOD, Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Blin
authors.affiliation: MMN, UMR CNRS 7083 Gulliver, ESPCI Paris, PSL Research University, 10 rue Vauquelin, 75005 Paris, France.|Sorbonne Universités, Université de Technologie de Compiègne, UMR CNRS 7338, BMBI, Compiègne France.
authors.forename: Anne
authors.initials: A
authors.lastname: Le Goff
authors.affiliation: PlatOD, Paris, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Magniez
authors.affiliation: PlatOD, Paris, France.|Inserm, UMR_S1140, Paris, France.
authors.forename: Sonia
authors.initials: S
authors.lastname: Poirault-Chassac
authors.affiliation: MMN, UMR CNRS 7083 Gulliver, ESPCI Paris, PSL Research University, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Teste
authors.affiliation: PlatOD, Paris, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Sicot
authors.affiliation: Inserm, UMR_S1148, Paris, France.
authors.forename: Kim Anh
authors.initials: KA
authors.lastname: Nguyen
authors.affiliation: PlatOD, Paris, France.
authors.forename: Feriel S
authors.initials: FS
authors.lastname: Hamdi
authors.affiliation: MMN, UMR CNRS 7083 Gulliver, ESPCI Paris, PSL Research University, 10 rue Vauquelin, 75005 Paris, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Reyssat
authors.affiliation: PlatOD, Paris, France.|Inserm, UMR_S1140, Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Baruch
mesh_terms: D015703:Antigens, CD / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D001696:Biomechanical Phenomena; D032701:Biomimetics; D019149:Bioreactors; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008533:Megakaryocytes / Q000166:cytology* / Q000502:physiology; D008954:Models, Biological; D015539:Platelet Activation / Q000502:physiology; D010974:Platelet Aggregation / Q000502:physiology; D010976:Platelet Count; D012212:Rheology; D013314:Stress, Mechanical
chemical_list: D015703:Antigens, CD; D015415:Biomarkers