title: Dyslipidemia-associated alterations in B cell subpopulation frequency and phenotype during experimental atherosclerosis.
abstract: Lymphocytes, the cellular effectors of adaptive immunity, are involved in the chronic inflammatory process known as atherosclerosis. Proatherogenic and atheroprotective properties have been ascribed to B cells. However, information regarding the role of B cells during atherosclerosis is scarce. Both the frequency and the phenotype of B cell subpopulations were studied by flow cytometry in wild type and apolipoprotein-E-deficient (apoE(-/-)) mice fed a high-fat (HFD) or control diet. Whereas the proportion of follicular cells was decreased, transitional 1-like cells were increased in mice with advanced atherosclerotic lesions (apoE(-/-) HFD). B cells in atherosclerotic mice were more activated, indicated by their higher surface expression of CD80, CD86, CD40 and CD95 and increased serum IgG1 levels. In the aorta, a decreased frequency of B cells was observed in mice with advanced atherosclerosis. Low expression of CD19 was observed on B cells from the spleen, aorta and lymph nodes of apoE(-/-) HFD mice. This alteration correlated with serum levels of IgG1 and cholesterol. A reduction in CD19 expression was induced in splenic cells from young apoE(-/-) mice cultured with lipemic serum. These results show that mice with advanced atherosclerosis display a variety of alterations in the frequency and phenotype of B lymphocytes, most of which are associated with dyslipidemia.
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70-52-21, Medellín, Colombia.
authors.forename: Héctor
authors.initials: H
authors.lastname: Rincón-Arévalo
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70-52-21, Medellín, Colombia.
authors.forename: Diana
authors.initials: D
authors.lastname: Castaño
authors.affiliation: Grupo Inmunomodulación, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70-52-21, Medellín, Colombia.
authors.forename: Janny
authors.initials: J
authors.lastname: Villa-Pulgarín
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70-52-21, Medellín, Colombia; Unidad de Citometría, Facultad de Medicina, Sede de Investigación Universitaria, Universidad de Antioquia UdeA, Calle 70-52-21, Medellín, Colombia.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rojas
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70-52-21, Medellín, Colombia.
authors.forename: Gloria
authors.initials: G
authors.lastname: Vásquez
authors.affiliation: Sección de Dermatología, Departamento de Medicina Interna, Facultad de Medicina, Universidad de Antioquia. Laboratorio Clínico VID, Obra de la Congregación Mariana, Medellín, Colombia.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Correa
authors.affiliation: Grupo Inmunomodulación, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Calle 70-52-21, Medellín, Colombia.
authors.forename: José R
authors.initials: JR
authors.lastname: Ramírez-Pineda
authors.affiliation: Grupo de Ciencias Básicas, Universidad CES, Medellín, Colombia. Electronic address: yascatorce@yahoo.com.
authors.forename: Lina M
authors.initials: LM
authors.lastname: Yassin
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000097:blood; D001011:Aorta / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D001018:Aortic Diseases / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D050171:Dyslipidemias / Q000097:blood / Q000235:genetics / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D020022:Genetic Predisposition to Disease; D007074:Immunoglobulin G / Q000097:blood; D016130:Immunophenotyping / Q000379:methods; D018655:Lymphocyte Count; D018345:Mice, Knockout; D010641:Phenotype
chemical_list: D015703:Antigens, CD; D001057:Apolipoproteins E; D015415:Biomarkers; D007074:Immunoglobulin G; D002784:Cholesterol
keywords: Apolipoprotein E; Atherosclerosis; B cell subsets; B cells; CD19; Cholesterol; Dyslipidemia