title: Denitrifying phosphorus removal from municipal wastewater and dynamics of "Candidatus Accumulibacter" and denitrifying bacteria based on genes of ppk1, narG, nirS and nirK.
abstract: Relevance of clade-level population dynamics of "Candidatus Accumulibacter" to performance of denitrifying phosphorus (P) removal from municipal wastewater was investigated. Stable denitrifying P removal in anoxic zone of continuous-flow reactor was achieved, accounting for 90% of total P removal. Clades IIC and IIF affiliated with Accumulibacter lineage were the dominant clades during denitrifying P removal, reaching 90% of ppk1 clone library. NarG gene library indicated Gamma and Beta-proteobacteria played an important role in nitrate reduction. Diversity and abundance of nirS library was much more than nirK, and thus became the main functional gene to execute nitrite reduction. Based on abundance of nirS, nirK and ppk1, the ratio of Accumulibacter capable of denitrifying P removal to total Accumulibacter was 22%. No matter whether Accumulibacter had narG gene or not, high abundance of narG at a level of 10(9)cells/(g dried-sludge) promoted nitrate reduced to nitrite, ensuring performance of denitrifying P removal.
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: zengwei_1@263.net.
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Anqi
authors.initials: A
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Environment Recovery, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D020562:Betaproteobacteria / Q000235:genetics / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D005798:Genes, Bacterial; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry / Q000382:microbiology; D062065:Wastewater / Q000737:chemistry*
chemical_list: D009566:Nitrates; D009573:Nitrites; D010758:Phosphorus; D012722:Sewage; D062065:Wastewater
keywords: Candidatus Accumulibacter; Denitrifying phosphorus removal; Municipal wastewater; Polyphosphate kinase 1 gene (ppk1); Population dynamics