title: Induction of cell self-organization on weakly positively charged surfaces prepared by the deposition of polyion complex nanoparticles of thermoresponsive, zwitterionic copolymers.
abstract: We have developed inducible cell self-organization through weakly positively charged culture surfaces. In this study, a thermoresponsive and zwitterionic copolymer comprised of N,N-dimethylaminoethyl methacrylate (DMAEMA) and methacrylic acid (MA) (PDMAEMA-co-PMA; Mn: ∼9.7 × 104g/mol; PDMAEMA/PMA ratio: 10) was designed for inducing cell self-organization. The copolymer formed single polymer-derived polyion complex (sPIC) nanoparticles following dissolution in an aqueous solution. The sPIC nanoparticles had a positive charge (ca. 25 mV). Self-organization occurred in adipose-derived vascular stromal cell monolayers cultivated on sPIC-deposited surfaces. There were dramatic morphological changes of these cells with the formation of capillary-like networks and single-cell aggregates with little cytotoxicity. This was a significant improvement compared with cells grown on previously developed surfaces deposited with PIC, a mixture of PDMAEMA and plasmid DNA. Thus, sPICs of PDMAEMA-co-PMA may allow for the accurate evaluation of a variety of cell behaviors with less cytotoxicity, and may facilitate additional potential medical applications such as cell-based therapy and drug discovery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1009-1015, 2017.
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Iwai
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Haruki
authors.affiliation: Development Department, Chemical Products Division, Bridgestone Co, Tokyo, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nemoto
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Nakayama
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D011109:Polymethacrylic Acids
keywords: cell self-organization; charged culture surface; multicellular spheroid; polyion complex; thermoresponsive and zwitterionic copolymer