title: Nanoghosts as a Novel Natural Nonviral Gene Delivery Platform Safely Targeting Multiple Cancers.
abstract: Nanoghosts derived from mesenchymal stem cells and retaining their unique surface-associated tumor-targeting capabilities were redesigned as a selective and safe universal nonviral gene-therapy platform. pDNA-loaded nanoghosts efficiently targeted and transfected diverse cancer cells, in vitro and in vivo, in subcutaneous and metastatic orthotopic tumor models, leading to no adverse effects. Nanoghosts loaded with pDNA encoding for a cancer-toxic gene inhibited the growth of metastatic orthotopic lung cancer and subcutaneous prostate cancer models and dramatically prolonged the animals' survival.
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Limor
authors.initials: L
authors.lastname: Kaneti
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Tomer
authors.initials: T
authors.lastname: Bronshtein
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Natali
authors.initials: N
authors.lastname: Malkah Dayan
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Inna
authors.initials: I
authors.lastname: Kovregina
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Nitzan
authors.initials: N
authors.lastname: Letko Khait
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Lupu-Haber
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Miguel
authors.initials: M
authors.lastname: Fliman
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Beth W
authors.initials: BW
authors.lastname: Schoen
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Galoz
authors.initials: G
authors.lastname: Kaneti
authors.affiliation: Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology , Haifa 3200003, Israel.
authors.forename: Marcelle
authors.initials: M
authors.lastname: Machluf
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics / Q000627:therapeutic use; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008810:Mice, Inbred C57BL; D049329:Nanostructures / Q000008:administration & dosage / Q000009:adverse effects / Q000648:ultrastructure; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology / Q000628:therapy; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics / Q000627:therapeutic use; D011467:Prostate / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*
chemical_list: D004247:DNA
keywords: mesenchymal stem cells; nanoghosts; nonviral gene therapy; targeted cancer therapy