title: The involvement of sirtuins during optic nerve injury of rats.
abstract: Sirtuins, comprised of seven members, protect cells from injury, possibly through different roles. In this study, we used two young rat optic nerve injury models to analyze the changes in Sirts 1-7 at different time points to better understand the role of sirtuins during optic nerve injury. Twelve-week-old adult male F344 rats (total n=42) were divided randomly into two groups. One group was subjected to optic nerve cut (ON-cut) and the other group was subjected to a peripheral nerve-optic nerve graft (PN-ON graft) on the left eye. At 1 and 3 days and 1, 2, and 4 weeks, rats were euthanized and retinas of both eyes were removed. Total RNA was extracted and first-strand cDNA was synthesized. Sirts 1-7 and housekeeping β-actin quantitative real-time PCR were performed. The quantitative real-time PCR profile showed that sirtuin mRNAs in both groups increased following optic nerve injury with and without peripheral nerve grafting. Sirt1 mRNA increased rapidly, reaching its peak at 3 days after surgery. Sirts 2-7 showed an increasing trend and remained high through 4 weeks after surgery. Sirts 4 and 6 were the only Sirts that increased in number in the PN-graft group at 4 weeks after surgery, where neuronal survival should be higher. Our data indicate that Sirt1 and Sirts 2-7 may play different or complementary roles in optic nerve injury and that Sirts 4 and 6 may play a greater role than the remaining Sirts in axon regeneration.
authors.affiliation: aDepartment of Pathology and Provincial Key Laboratory of Infectious Diseases and Immunopathology, Collaborative and Creative Center, Shantou University Medical College bJoint Shantou International Eye Center, Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China cDepartment of Genetics, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Pei
authors.initials: P
authors.lastname: Meng
authors.forename: Jiacong
authors.initials: J
authors.lastname: Wei
authors.forename: Jingying
authors.initials: J
authors.lastname: Wang
authors.forename: Jiajian
authors.initials: J
authors.lastname: Liang
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhi
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Geng
mesh_terms: D000818:Animals; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D020221:Optic Nerve Injuries / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D060888:Real-Time Polymerase Chain Reaction; D037761:Sirtuins / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D037761:Sirtuins