title: Oxidative Stress and Cell Apoptosis in Caprine Liver Induced by Molybdenum and Cadmium in Combination.
abstract: To investigate the effects of co-exposure to molybdenum (Mo) and cadmium (Cd) on oxidative stress and cell apoptosis in caprine livers, 36 Boer goats were randomly divided into four groups with nine goats in each group. Three groups were randomly assigned with one of three oral treatments of CdCl2 (0.5 mg Cd kg(-1)·BW) and [(NH4)6Mo7O24·4H2O] (15 mg Mo kg(-1)·BW, 30 mg Mo kg(-1)·BW, 45 mg Mo kg(-1)·BW), while the control group received deionized water. Liver tissues on days 0, 25, and 50 were subjected to determine antioxidant activity indexes and the messenger RNA (mRNA) expression levels of ceruloplasmin (CP), cysteinyl aspartate-specific proteinase-3 (caspase-3), second mitochondria-derived activator of caspases (Smac), and cytochrome-C (Cyt-C) genes. The results showed that significant reductions were observed in total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activities (P < 0.05), while activities or contents of malondialdehyde (MDA), nitric oxide (NO), and nitric oxide synthase (NOS) were increased (P < 0.05). The mRNA expression levels of CP, caspase-3, Smac, and Cyt-C genes were upregulated (P < 0.05). In addition, histopathological lesions showed different degrees of vacuolar degeneration and edematous and mitochondrial swelling. The results suggest that co-exposure to Mo and Cd could induce oxidative stress and cell apoptosis possibly associated with mitochondrial intrinsic pathway in goat liver and show possible synergistic effects between the two elements.
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China.
authors.forename: Caiying
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China.
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China. hgljx3818@163.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agriculture University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, People's Republic of China. chbin20020804@163.com.
authors.forename: Huabin
authors.initials: H
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002104:Cadmium / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006041:Goats; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008982:Molybdenum / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D002104:Cadmium; D008982:Molybdenum
keywords: Apoptosis; Cadmium; Goat; Liver; Molybdenum