title: Microsatellite instability of gastric cancer and precancerous lesions.
abstract: OBJECTIVE
  To investigate whether microsatellite instability (MSI) of gastric cancer and precancerous lesions were existed and its effect.
  
  
  METHODS
  Laser microdissection was used. Gastric, intestinal metaplasia, dysplasia and normal mucosa were collected respectively. Five microsatellite loci were selected and MSI was detected by denaturing high-performance liquid chromatography.
  
  
  RESULTS
  In the five microsatellite loci REF-positive phenotype, intestinal metaplasia MSI was 20.7%. Dysplasia MSI was 22.4%. Gastric MSI was 47.9%, and there was no MSI in normal gastric mucosa.
  
  
  CONCLUSIONS
  MSI gradually increased from precancerous lesions to gastric cancer. The early detection of MSI may be a potential early warning indicator for early diagnosis of gastric cancer.
authors.affiliation: Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Guo
authors.affiliation: Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.affiliation: Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.
authors.forename: Fang-Ming
authors.initials: FM
authors.lastname: Gong
authors.affiliation: Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.
authors.forename: Bao-Qing
authors.initials: BQ
authors.lastname: Jia
keywords: Gastric cancer; denaturing high performance liquid chromatography; laser micro dissection; microsatellite instability; precancerous lesions