title: Association of antibiotic resistance with SHV-12 extended-spectrum β-lactamase inEnterobacter cloacae.
abstract: The association between antibiotic resistance and SHV-12 extended-spectrum β-lactamase (ESBL) inEnterobacter cloacaeremains unknown. The aim of the present study was to investigate the prevalence of both chromosome- and plasmid-borne SHV-12 ESBL genes inEnterobacter cloacae.Transmission of the SHV-12 ESBL gene was explored, and the risk factors for antibiotic resistance inE. cloacaewere analyzed. Polymerase chain reaction (PCR) results showed that 58 out of the 100 isolates carried the SHV-12 ESBL gene: 34.48% of them occurred in the chromosome, 48.28% were plasmid-borne and 17.24% appeared in both. Enterobacterial repetitive intergenic consensus-PCR tests detected 82 chromosomal genotypes. Conjugation assays showed that 70.00% of plasmid-borne SHV-12 ESBL genes were successfully transconjugated intoE. coliC600 and that the antibiotic resistance phenotype ofE. cloacaewas partially (84%) or completely (10%) transferred. A significantly higher SHV-12 ESBL detection rate was found in patients with underlying conditions and/or complications compared with those without (P<0.05). The detection of SHV-12 ESBL-producingE. cloacaefrom vertical transmission varied significantly across clinical departments and age groups (P<0.05), with the highest rates in the intensive care unit and the group of patients aged ≥60 years. The present results indicate that the location and transmission efficiency of SHV-12 ESBL are closely correlated with the antibiotic resistance ofE. cloacae.
authors.affiliation: Laboratory of Pathogenic Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Pathogenic Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Guo-Ming
authors.initials: GM
authors.lastname: Li
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524000, P.R. China.
authors.forename: Li-Yao
authors.initials: LY
authors.lastname: Lin
authors.affiliation: Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524000, P.R. China.
authors.forename: Xia-Lei
authors.initials: XL
authors.lastname: Wu
authors.affiliation: Department of Experimental Medicine, Taiping People's Hospital of Dongguan, Dongguan, Guangdong 523095, P.R. China.
authors.forename: Shao-Long
authors.initials: SL
authors.lastname: Huang
authors.affiliation: Department of Experimental Medicine, Taiping People's Hospital of Dongguan, Dongguan, Guangdong 523095, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Laboratory of Pathogenic Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
authors.forename: Zu-Guo
authors.initials: ZG
authors.lastname: Zhao
keywords: Enterobacter cloacae; Phenotype; SHV-12; enterobacterial repetitive intergenic consensus-polymerase chain reaction; extended spectrum β-lactamases