title: Anti-inflammatory and anti-apoptotic effects of oxysophoridine on lipopolysaccharide-induced acute lung injury in mice.
abstract: Oxysophoridine (OSR) is an alkaloid with multiple pharmacological activities. This study aimed to investigate the protective effects and underlying mechanisms of OSR on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Here, we found that OSR treatment markedly mitigated LPS-induced body weight loss and significant lung injury characterized by the deterioration of histopathology, histologic scores, wet-to-dry ratio, exduate volume, and protein leakage. OSR dramatically attenuated LPS-induced lung inflammation, as evidenced by the reduced levels of total cells, neutrophils, lymphocytes, and macrophages and pro-inflammatory cytokines (i.e., tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and monocyte chemoattractant protein-1) in bronchoalveolar lavage fluid and of their mRNA expression in lung tissues. OSR also inhibited LPS-induced expression and activation of nuclear factor-κB p65 in pulmonary tissue. Additionally, OSR administration markedly prevented LPS-induced pulmonary cell apoptosis in mice, as reflected by the decrease in expression of procaspase-8, procaspase-3, cleaved caspase-8, and cleaved caspase-3, and Bcl-2-associated X/B-cell lymphoma 2 ratio. These results indicate that OSR is a potential therapeutic drug for treating LPS-induced ALI.
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan 453100, PR China.
authors.forename: Junjing
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Intensive Care Unit, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan 453100, PR China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan 453100, PR China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan 453100, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Emergency Medicine, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan 453100, PR China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan 453100, PR China.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Yang
keywords: Acute lung injury; apoptosis; inflammation; lipopolysaccharide; oxysophoridine