title: Development of biomimetic nanocomposites as bone extracellular matrix for human osteoblastic cells.
abstract: Here, we have developed biomimetic nanocomposites containing chitosan, poly(vinyl alcohol) and nano-hydroxyapatite-zinc oxide as bone extracellular matrix for human osteoblastic cells and characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction. Scanning electron microscopy images revealed interconnected macroporous structures. Moreover, in this study, the problem related to fabricating a porous composite with good mechanical strength has been resolved by incorporating 5wt% of nano-hydroxyapatite-zinc oxide into chitosan-poly(vinyl alcohol) matrix; the present composite showed high tensile strength (20.25MPa) while maintaining appreciable porosity (65.25%). These values are similar to human cancellous bone. These nanocomposites also showed superior water uptake, antimicrobial and biodegradable properties than the previously reported results. Compatibility with human blood and pH was observed, indicating nontoxicity of these materials to the human body. Moreover, proliferation of osteoblastic MG-63 cells onto the nanocomposites was also observed without having any negative effect.
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
authors.forename: Arundhati
authors.initials: A
authors.lastname: Bhowmick
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
authors.forename: Tapas
authors.initials: T
authors.lastname: Mitra
authors.affiliation: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Gnanamani
authors.affiliation: Department of Chemical Engineering, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.
authors.forename: Manas
authors.initials: M
authors.lastname: Das
authors.affiliation: Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India. Electronic address: ppk923@yahoo.com.
authors.forename: Patit Paban
authors.initials: PP
authors.lastname: Kundu
mesh_terms: D000890:Anti-Infective Agents / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D001409:Bacillus cereus / Q000187:drug effects; D032701:Biomimetics; D002460:Cell Line; D048271:Chitosan / Q000031:analogs & derivatives; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D006882:Hydroxyapatites / Q000737:chemistry; D053761:Nanocomposites / Q000009:adverse effects / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D006882:Hydroxyapatites; D011142:Polyvinyl Alcohol; D048271:Chitosan; D015034:Zinc Oxide
keywords: Chitosan; Hydroxyapatite; Nanocomposites; Polyvinyl alcohol; Zinc-oxide