title: Hydrogen peroxide-induced apoptosis in human gingival fibroblasts.
abstract: In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and ulceration of the gingival or other soft tissues. The aim of the present study was to determine the effect of hydrogen peroxide on apoptosis in human gingival fibroblasts (HGF). Cytochrome c, Bcl-2, Bax, Bid and caspase-3 protein expression were detected by Western blotting. HGF cell apoptosis induced by H2O2 was both dose and time dependent. The addition of H2O2 resulted in the release of cytochrome c to the cytosol, and an increase of Caspase-3 cleavage. Data suggest that oxidative stress-induced apoptosis in HGF is intrinsic pathway involved the release of apoptotic signal from mitochondria.
authors.affiliation: Laboratorio de Bioquímica de la División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Ciudad Universitaria D.F., México.
authors.forename: Gloria
authors.initials: G
authors.lastname: Gutiérrez-Venegas
authors.affiliation: Laboratorio de Bioquímica de la División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Ciudad Universitaria D.F., México.
authors.forename: Adriana
authors.initials: A
authors.lastname: Guadarrama-Solís
authors.affiliation: Laboratorio de Bioquímica de la División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Ciudad Universitaria D.F., México.
authors.forename: Carmen
authors.initials: C
authors.lastname: Muñoz-Seca
authors.affiliation: Laboratorio de Bioquímica de la División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Ciudad Universitaria D.F., México.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Arreguín-Cano
mesh_terms: D000293:Adolescent; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005881:Gingiva / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D013997:Time Factors; D058205:Tooth Bleaching Agents / Q000633:toxicity*; D055815:Young Adult
chemical_list: D051017:Apoptosis Regulatory Proteins; D058205:Tooth Bleaching Agents; D006861:Hydrogen Peroxide
keywords: Human gingival fibroblasts; apoptosis; discolored teeth; hydrogen peroxide