title: Characterization and functional analysis of two inhibitor of apoptosis genes in Zhikong scallop Chlamys farreri.
abstract: The proteins of inhibitor of apoptosis (IAP) family play important roles in regulation of apoptosis, immunological response and cell proliferation. Here we reported two IAP genes (named CfIAP1 and CfIAP2) in Zhikong scallop Chlamys farreri. The full-length CfIAP1 cDNA contained 1552 nucleotides, encoding a predicted protein of 251 amino acids with two BIR domains. The full-length CfIAP2 cDNA contained 1243 nt, encoding a 356-aa protein with one BIR domain and one RING domain. The two genes are ubiquitously expressed in six types of tissue of C. farreri. The expression levels of CfIAP1 and CfIAP2 were significantly up-regulated after challenged with acute viral necrobiotic disease virus, lipopolysaccharide and exposure to air. Subcellular localization assay showed that CfIAP1 was mainly distributed in cytoplasm and CfIAP2 was in cytoplasm and nucleus. As assessed using a kit designed to test Caspase3 function in mammalian cells, the activity of CfCaspase3 was enhanced as a result of the down-regulation of CfIAP2 expression by dsRNA-mediated gene silencing. Our study indicated that CfIAP1 and CfIAP2 may participate in the innate immunity and stress responses and that CfIAP2 might block apoptosis via inhibiting CfCaspase3 indirectly through an unexplored mechanism in C. farreri.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Miao
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Haigang
authors.initials: H
authors.lastname: Qi
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: hque@qdio.ac.cn.
authors.forename: Huayong
authors.initials: H
authors.lastname: Que
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: gzhang@qdio.ac.cn.
authors.forename: Guofan
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D015687:Cell Hypoxia; D002467:Cell Nucleus / Q000378:metabolism; D017124:Conserved Sequence; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007113:Immunity, Innate; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009928:Organ Specificity; D049897:Pectinidae / Q000166:cytology / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010802:Phylogeny; D000072417:Protein Domains; D021381:Protein Transport; D015533:Transcriptional Activation / Q000276:immunology
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D008070:Lipopolysaccharides
keywords: Apoptosis; Chlamys farreri; Inhibitor of apoptosis; Innate immunity; Zhikong scallop