title: Evaluation of the Goldmann-Witmer coefficient in the immunological diagnosis of ocular toxocariasis.
abstract: Ocular toxocariasis (OT) is a zoonotic parasitic infection mainly caused by the intraocular tissue invasion of second-stage Toxocara canis or Toxocara cati larva. Measuring specific anti-T. canis antibodies in the intraocular fluid (IF) can increase OT diagnosis accuracy using Goldmann-Witmer coefficient (GWC). However, there is no systemic evaluation of GWC application in the immunological diagnosis of OT. To assess GWC for the immunodiagnosis of ocular toxocariasis, paired IF and serum samples from 72 patients diagnosed with OT were retrospectively analyzed for specific anti-T. canis IgG by enzyme linked immunosorbent assay (ELISA). GWC values were calculated to determine intraocular specific IgG production. Clinical features and other laboratory data were recorded, and their correlations with GWC evaluated. Of the 72 OT patients, 60 (83.33%) showed intraocular specific IgG production confirmed by GWC, while intraocular nonspecific IgE production was found in 64/69 (92.75%) cases. No significant correlation was found among clinical features and IF specific IgG production. Values for peripheral blood samples were lower than those of intraocular fluid regarding OT screening, and vitreous humor samples showed increased local specific IgG and nonspecific IgE production compared with aqueous humor samples. Overall, our results indicate that GWC and intraocular IgE production have referential values in diagnosing ocular toxocariasis.
authors.affiliation: Clinical Laboratory, Fudan University Eye and ENT Hospital, Shanghai 200031, China.
authors.forename: Zhu Jian
authors.initials: ZJ
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, Fudan University Eye and ENT Hospital, Shanghai 200031, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Clinical Laboratory, Fudan University Eye and ENT Hospital, Shanghai 200031, China. Electronic address: 18917785189@163.com.
authors.forename: Wen Jun
authors.initials: WJ
authors.lastname: Cao
authors.affiliation: Clinical Laboratory, Fudan University Eye and ENT Hospital, Shanghai 200031, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.affiliation: Department of Pathology, Fudan University Eye and ENT Hospital, Shanghai 200031, China.
authors.forename: Ying Wen
authors.initials: YW
authors.lastname: Bi
authors.affiliation: Department of Ophthalmology, Fudan University Eye and ENT Hospital, Shanghai 200031, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Ophthalmology, Fudan University Eye and ENT Hospital, Shanghai 200031, China.
authors.forename: Ge Zhi
authors.initials: GZ
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001082:Aqueous Humor / Q000469:parasitology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015822:Eye Infections, Parasitic / Q000175:diagnosis* / Q000276:immunology*; D005260:Female; D006801:Humans; D007159:Immunologic Tests; D007223:Infant; D007815:Larva Migrans; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000175:diagnosis* / Q000276:immunology*; D055815:Young Adult
keywords: Enzyme-linked immunosorbent assay; Intraocular fluid; Ocular toxocariasis