title: Efficient conversion of crude glycerol from various industrial wastes into single cell oil by yeast Yarrowia lipolytica.
abstract: In this study, crude glycerol from various industries was used to produce lipids via wild type Yarrowia lipolytica A101. We tested samples without any prior purification from five different waste products; each contained various concentrations of glycerol (42-87%) as the sole carbon source. The best results for lipid production were obtained for medium containing glycerol from fat saponification. This reached 1.69gL(-1) (25% of total cell dry weight) with a biomass yield of 0.17gg(-1) in the flasks experiment. The batch cultivation in a bioreactor resulted in enhanced lipid production-it achieved 4.72gL(-1) with a biomass yield 0.21gg(-1). Moreover, the properly selected batch of crude glycerol provides a defined fatty acid composition. In summary, this paper shows that crude glycerol from soap production could be efficiently converted to single cell oil without any prior purification.
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Dobrowolski
authors.affiliation: Institute of Environmental Engineering, Wrocław University of Environmental and Life Sciences, Grunwaldzki Sq 24, Wrocław 50-363, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Mituła
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rymowicz
authors.affiliation: Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego 37, Wrocław 51-630, Poland. Electronic address: aleksandra.mironczuk@up.wroc.pl.
authors.forename: Aleksandra M
authors.initials: AM
authors.lastname: Mirończuk
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D007220:Industrial Waste / Q000032:analysis*; D009584:Nitrogen / Q000378:metabolism; D025062:Yarrowia / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D007220:Industrial Waste; D002244:Carbon; D009584:Nitrogen; D005990:Glycerol
keywords: Industrial wastes; Microbial oil; Oleaginous yeast; Yarrowia lipolytica