title: Profile of Steroid Receptors and Increased Aromatase Immunoexpression in Canine Inflammatory Mammary Cancer as a Potential Therapeutic Target.
abstract: Canine inflammatory mammary cancer (IMC) has been proposed as a model for the study of human inflammatory breast cancer (IBC). The aims of this study were to compare the immunohistochemical expression of aromatase (Arom) and several hormone receptors [estrogen receptor α (ERα), estrogen receptor β (ERβ), progesterone receptor (PR) and androgen receptor (AR)], in 21 IMC cases vs 19 non-IMC; and to study the possible effect of letrozole on canine IMC and human inflammatory breast cancer (IBC) in vitro using IPC-366 and SUM-149 cell lines. Significant elevations of the means of Arom Total Score (TS), ERβ TS and PR TS were found in the IMC group (p = 0.025, p = 0.038 and p = 0.037, respectively). Secondary IMC tumours expressed higher levels of Arom than primary IMC (p = 0.029). Non-IMC PR- tumours contained higher levels of Arom than non-IMC PR+ tumours (p = 0.007). After the addition of letrozole, the number of IMC and IBC cells dropped drastically. The overexpression of Arom found and the results obtained in vitro further support canine IMC as a model for the study of IBC and future approaches to the treatment of dogs with mammary cancer, and especially IMC, using Arom inhibitors.
authors.affiliation: Department of Animal Medicine Surgery and Pathology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
authors.forename: P J
authors.initials: PJ
authors.lastname: De Andrés
authors.affiliation: Department of Animal Physiology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Cáceres
authors.affiliation: Department of Animal Medicine Surgery and Pathology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Clemente
authors.affiliation: Department of Animal Medicine Surgery and Pathology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Pérez-Alenza
authors.affiliation: Department of Animal Physiology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Illera
authors.affiliation: Department of Animal Medicine Surgery and Pathology, Veterinary Medicine School, Complutense University of Madrid, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Peña
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D000077289:Letrozole; D015674:Mammary Neoplasms, Animal / Q000201:enzymology* / Q000378:metabolism; D009570:Nitriles / Q000008:administration & dosage / Q000494:pharmacology; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism*; D014230:Triazoles / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D009570:Nitriles; D011987:Receptors, Steroid; D014230:Triazoles; D000077289:Letrozole; D001141:Aromatase