title: Molecularly imprinted plasmonic nanosensor for selective SERS detection of protein biomarkers.
abstract: Molecularly imprinted plasmonic nanosensor has been prepared via the rational design of an ultrathin polymer layer on the surface of gold nanorods imprinted with the target protein. This nanosensor enabled selective fishing-out of the target protein biomarker even from a complex real sample such as human serum. Sensitive SERS detection of the protein biomarkers with a strong Raman enhancement was achieved by formation of protein imprinted gold nanorods aggregates, stacking of protein imprinted gold nanorods onto a glass plate, or self-assembly of protein imprinted gold nanorods into close-packed array. High specificity and sensitivity of this method were demonstrated with a detection limit of at least 10(-8)mol/L for the target protein. This could provide a promising alternative for the currently used immunoassays and fluorescence detection, and offer an ultrasensitive, non-destructive, and label-free technique for clinical diagnosis applications.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: lvyq@mail.buct.edu.cn.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Qin
authors.affiliation: International Research Center for Soft Matter, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: twtan@mail.buct.edu.cn.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
mesh_terms: D015415:Biomarkers / Q000097:blood*; D015374:Biosensing Techniques; D001798:Blood Proteins / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D006801:Humans; D054802:Molecular Imprinting; D036103:Nanotechnology; D043942:Nanotubes / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D011506:Proteins; D006046:Gold
keywords: Gold nanorods; Molecular imprinting; Nanosensor; Protein biomarker; Surface-enhanced Raman scattering