title: Molecular cloning and functional analysis of nucleotide-binding oligomerization domain-containing protein 1 in rainbow trout, Oncorhynchus mykiss.
abstract: NOD1 has important roles in innate immunity as sensor of microbial components derived from bacterial peptidoglycan. In this study, we identified genes encoding components of the NOD1 signaling pathway, including NOD1 (OmNOD1) and RIP2 (OmRIP2) from rainbow trout, Oncorhynchus mykiss, and investigated whether OmNOD1 has immunomodulating activity in a rainbow trout hepatoma cell line RTH-149 treated with NOD1-specific ligand (iE-DAP). The deduced amino acid sequence of OmNOD1 contained conserved CARD, NOD and LRR domains. Loss-of-function and gain-of-function experiments indicated that OmNOD1 is involved in the expression of pro-inflammatory cytokines. Silencing of OmNOD1 in RTH-149 cells treated with iE-DAP decreased the expression of IL-1β, IL-6, IL-8 and TNF-α. Conversely, overexpression of OmNOD1 resulted in up-regulation of IL-1β, IL-6, IL-8 and TNF-α expression. In addition, RIP2 inhibitor (gefitinib) significantly decreased the expression of these pro-inflammatory cytokines induced by iE-DAP in RTH-149 cells. These findings highlight the important role of NOD1 signaling pathway in fish in eliciting innate immune response.
authors.affiliation: Research Institute of Life Science, Gyeongsang National University, Jinju 52828, South Korea.
authors.forename: Ju Hye
authors.initials: JH
authors.lastname: Jang
authors.affiliation: Division of Life Science, Gyeongsang National University, Jinju 52828, South Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Division of Life Science, Gyeongsang National University, Jinju 52828, South Korea.
authors.forename: Yu Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Research Institute of Life Science, Gyeongsang National University, Jinju 52828, South Korea; Division of Life Science, Gyeongsang National University, Jinju 52828, South Korea. Electronic address: juhyun.cho@gnu.ac.kr.
authors.forename: Ju Hyun
authors.initials: JH
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007113:Immunity, Innate; D056747:Immunomodulation; D008024:Ligands; D053474:Nod1 Signaling Adaptor Protein / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D029941:Fish Proteins; D008024:Ligands; D053474:Nod1 Signaling Adaptor Protein
keywords: Innate immunity; Nucleotide-binding oligomerization domain-containing protein 1; RTH-149; Rainbow trout; Receptor-interacting serine/threonine-protein kinase 2; Teleost