title: One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus.
abstract: 'Torrado' disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.
authors.affiliation: Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Władysława Wegorka 20, 60-318, Poznan, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Budziszewska
authors.affiliation: Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Władysława Wegorka 20, 60-318, Poznan, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Wieczorek
authors.affiliation: Interdepartmental Laboratory of Molecular Biology, Institute of Plant Protection-National Research Institute, Władysława Wegorka 20, 60-318, Poznan, Poland. olaob@o2.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Obrępalska-Stęplowska
mesh_terms: D001483:Base Sequence; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010849:Picornaviridae / Q000235:genetics*; D010850:Picornaviridae Infections / Q000175:diagnosis*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment
chemical_list: D012367:RNA, Viral
keywords: Diagnostic protocol; Plant virus detection; RT-LAMP; Torradovirus; Virus diagnostics