title: Metal (Ag, Cd, Cu, Ni, Tl, and Zn) Binding to Cytosolic Biomolecules in Field-Collected Larvae of the Insect Chaoborus.
abstract: We characterized the biomolecules involved in handling cytosolic metals in larvae of the phantom midge (Chaoborus) collected from five mining-impacted lakes by determining the distribution of Ag, Cd, Cu, Ni, Tl, and Zn among pools of various molecular weights (HMW: high molecular weight, >670-40 kDa; MMW: medium molecular weight, 40-<1.3 kDa; LMW: low molecular weight, <1.3 kDa). Appreciable concentrations of nonessential metals were found in the potentially metal-sensitive HMW (Ag and Ni) and LMW (Tl) pools, whereas the MMW pool, which includes metallothioneins (MTs) and metallothionein-like proteins and peptides (MTLPs), appears to be involved in Ag and Cd detoxification. Higher-resolution fractionation of the heat-stable protein (HSP) fraction revealed further differences in the partitioning of nonessential metals (i.e., Ag = Cd ≠ Ni ≠ Tl). These results provide unprecedented details about the metal-handling strategies employed by a metal-tolerant, freshwater animal in a field situation.
authors.affiliation: Institut National de la Recherche Scientifique - Centre Eau Terre Environnement (INRS-ETE) , 490 de la Couronne, Quebec City, Quebec, G1K 9A9 Canada.
authors.forename: Maikel
authors.initials: M
authors.lastname: Rosabal
authors.affiliation: CNRS/UPPA, Laboratoire de Chimie Analytique Bio-Inorganique et Environnement UMR 5254 , Hélioparc, 2. Av. Pr. Angot, Pau, 64053 France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Mounicou
authors.affiliation: Institut National de la Recherche Scientifique - Centre Eau Terre Environnement (INRS-ETE) , 490 de la Couronne, Quebec City, Quebec, G1K 9A9 Canada.
authors.forename: Landis
authors.initials: L
authors.lastname: Hare
authors.affiliation: Institut National de la Recherche Scientifique - Centre Eau Terre Environnement (INRS-ETE) , 490 de la Couronne, Quebec City, Quebec, G1K 9A9 Canada.
authors.forename: Peter G C
authors.initials: PG
authors.lastname: Campbell
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D004175:Diptera / Q000187:drug effects* / Q000378:metabolism; D004784:Environmental Monitoring; D019476:Insect Proteins / Q000378:metabolism; D060106:Lakes; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D008670:Metals / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics; D008970:Molecular Weight; D009864:Ontario; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D008670:Metals; D014874:Water Pollutants, Chemical; D008668:Metallothionein