title: Effects of Dietary Selenium Supplementation on Seminiferous Tubules and SelW, GPx4, LHCGR, and ACE Expression in Chicken Testis.
abstract: We investigated the effects of dietary selenium (Se) supplementation on the development of chicken testis and the expression of selenoprotein W (SelW), glutathione peroxidase4 (GPx4), luteinizing hormone/choriogonadotropin receptor (LHCGR), and angiotensin converting enzyme (ACE). Sixty roosters were assigned randomly into the control group fed with a basic diet (containing 0.3 mg Se/kg) and the experimental group fed with a diet (containing 0.6 mg Se/kg). The testes were collected individually at age of 6, 9, and 12 weeks. Se was supplemented in chicken feed for 15 days before sampling. The results indicated that dietary Se affected the number of cells in the seminiferous tubules and viability of Sertoli cells in vitro culture. SelW and GPx4 expression in the testes increased significantly in the experimental group compared to that in the control group. LHCGR expression in the testes increased significantly in the experimental group after 12 weeks compared to that in the control group. In contrast, ACE expression was inhibited in the experimental group compared to that in the control group. These results suggest that dietary supplementation with Se improved development of the seminiferous tubules at the cellular level and that SelW, GPx4, LHCGR, and ACE are involved.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China.|Department of Animal Production, College of Agriculture, University of Tikrit, Tikrit, Iraq.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Khalid
authors.affiliation: Key laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin, 150030, China.|Biology Department, Education College for Women, Al-Anbar University, Ramadi, 31001, Iraq.
authors.forename: Nagam
authors.initials: N
authors.lastname: Khudhair
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Huang
authors.initials: H
authors.lastname: He
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Peng
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yaguang
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China. gxzhang@neau.edu.cn.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Guixue
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis*; D002645:Chickens / Q000378:metabolism*; D019587:Dietary Supplements; D005786:Gene Expression Regulation / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000096:biosynthesis*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000096:biosynthesis*; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D011974:Receptors, LH / Q000096:biosynthesis*; D012643:Selenium / Q000494:pharmacology*; D051151:Selenoprotein W / Q000096:biosynthesis*; D012671:Seminiferous Tubules / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D011974:Receptors, LH; D051151:Selenoprotein W; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D007703:Peptidyl-Dipeptidase A; D012643:Selenium
keywords: Chicken; Gene; Male reproduction; Selenium; Sertoli cell