title: Bioactive cell-like hybrids coassembled from (glyco)dendrimersomes with bacterial membranes.
abstract: A library of amphiphilic Janus dendrimers including two that are fluorescent and one glycodendrimer presenting lactose were used to construct giant dendrimersomes and glycodendrimersomes. Coassembly with the components of bacterial membrane vesicles by a dehydration-rehydration process generated giant cell-like hybrid vesicles, whereas the injection of their ethanol solution into PBS produced monodisperse nanometer size assemblies. These hybrid vesicles contain transmembrane proteins including a small membrane protein, MgrB, tagged with a red fluorescent protein, lipopolysaccharides, and glycoproteins from the bacterium Escherichia coli. Incorporation of two colored fluorescent probes in each of the components allowed fluorescence microscopy to visualize and demonstrate coassembly and the incorporation of functional membrane channels. Importantly, the hybrid vesicles bind a human galectin, consistent with the display of sugar moieties from lipopolysaccharides or possibly glycosylated membrane proteins. The present coassembly method is likely to create cell-like hybrids from any biological membrane including human cells and thus may enable practical application in nanomedicine.
authors.affiliation: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6313;
authors.forename: Srujana S
authors.initials: SS
authors.lastname: Yadavalli
authors.affiliation: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;
authors.forename: Shaodong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;
authors.forename: Samuel E
authors.initials: SE
authors.lastname: Sherman
authors.affiliation: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;
authors.forename: Elodie
authors.initials: E
authors.lastname: Fiorin
authors.affiliation: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;
authors.forename: Louise
authors.initials: L
authors.lastname: da Silva
authors.affiliation: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323;
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Wilson
authors.affiliation: Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321; Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104-6391;
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Hammer
authors.affiliation: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilian University, 80539 Munich, Germany;
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.affiliation: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilian University, 80539 Munich, Germany;
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
authors.affiliation: Institute of Computational Molecular Science, Temple University, Philadelphia, PA 19122 mlklein@temple.edu goulian@sas.upenn.edu percec@sas.upenn.edu.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Klein
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6313; mlklein@temple.edu goulian@sas.upenn.edu percec@sas.upenn.edu.
authors.forename: Mark
authors.initials: M
authors.lastname: Goulian
authors.affiliation: Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323; mlklein@temple.edu goulian@sas.upenn.edu percec@sas.upenn.edu.
authors.forename: Virgil
authors.initials: V
authors.lastname: Percec
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D050091:Dendrimers / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*
chemical_list: D050091:Dendrimers
keywords: E. coli; galectin; lipopolysaccharides; transmembrane protein