title: Landfill leachate treatment using bacto-algal co-culture: An integrated approach using chemical analyses and toxicological assessment.
abstract: The present study aims to evaluate the feasibility of leachate treatment using a synergistic approach by microalgae and bacteria. Leachate from one of the landfill of Northern India showed the presence of various toxic organic contaminants like naphthalene, benzene, phenol and their derivatives, napthols, pesticides, epoxides, phthalates and halogenated organic compounds. ICP-AES analysis revealed high concentrations of Zn, Cr, Fe, Ni, and Pb beyond the maximum permissible limit of discharge. Bacto-algal co-culture was found to be the most efficient in removal of toxic organic contaminants and heavy metals. Further, detoxification efficiency of bacto-algal treatment was evaluated by Methyl tetrazolium (MTT) assay for cytotoxicity and alkaline comet assay for genotoxicity using hepatoma HepG2 cells. Reduction in toxicity was confirmed by an increase in LC50 by 1.9 fold and reduction in Olive Tail Moment by 40.6 fold after 10 days of treatment. Results of the study indicate bioremediation and detoxification potency of bacto-algal co-culture for leachate treatment.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: mona.jnu10@gmail.com.
authors.forename: Moni
authors.initials: M
authors.lastname: Kumari
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: isthakur@hotmail.com.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D020552:Comet Assay; D056945:Hep G2 Cells; D006801:Humans; D007194:India; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D058086:Microalgae / Q000378:metabolism*; D009930:Organic Chemicals / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity; D064091:Waste Disposal Facilities; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D019216:Metals, Heavy; D009930:Organic Chemicals; D014874:Water Pollutants, Chemical
keywords: Co-culture; Detoxification; Landfill leachate; Lysimeter; Treatment