title: Tandem repeat knockout utilizing the CRISPR/Cas9 system in human cells.
abstract: Tandem repeats have been shown to cause human genetic diseases and contribute significantly to genome variation and instability. Although multi-sgRNAs mediated CRISPR/Cas9 system have used to generate regional deletions previously, in this study we explored a method of generating regional deletions of tandem repeats by taking advantage of the off-target effects of CRISPR/Cas9 in 293FT cells. Our results revealed that generation of large-fragment deletions of tandem repeats located in the MAGEL2 and XIST gene was possible. In summary, we have demonstrated that large-fragment deletions of tandem repeats can be achieved using a sgRNA-directed CRISPR/Cas9 system, facilitating the functional study of tandem repeats in future studies.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, 130062, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Lv
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, 130062, China.
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, 130062, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, 130062, China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Song
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, 130062, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Sui
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun, 130062, China. Electronic address: lizj_1998@jlu.edu.cn.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D064113:CRISPR-Cas Systems / Q000235:genetics*; D055786:Gene Knockout Techniques; D005822:Genetic Vectors / Q000378:metabolism; D015894:Genome, Human; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems; D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA
keywords: CRISPR/Cas9; Off-target mutation; Regional deletions; Tandem repeats