title: Antibacterial activity of isolated phenolic compounds from cranberry (Vaccinium macrocarpon) against Escherichia coli.
abstract: Phenolic compounds from a cranberry extract were isolated in order to assess their contribution to the antibacterial activity against uropathogenic strains of Escherichia coli (UPEC). With this purpose, a total of 25 fractions from a cranberry extract were isolated using semipreparative high performance liquid chromatography (HPLC) and characterized based on the results obtained by reversed-phase HPLC coupled to mass spectrometry detection. Then, the effects on UPEC surface hydrophobicity and biofilm formation of the cranberry extract as well as the purest fractions (a total of 13) were tested. As expected, the whole extract presented a powerful antibacterial activity against UPEC while the selected fractions presented a different behavior. Myricetin and quercitrin significantly decreased (p < 0.05) E. coli biofilm formation compared with the control, while dihydroferulic acid glucuronide, procyanidin A dimer, quercetin glucoside, myricetin and prodelphinidin B led to a significant decrease of the surface hydrophobicity compared with the control. The results suggest that apart from proanthocyanidins, other compounds, mainly flavonoids, can act against E. coli biofilm formation and also modify UPEC surface hydrophobicity in vitro, one of the first steps of adhesion.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain and Research and Development Functional Food Center (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. rquirantes@ugr.es.
authors.forename: Celia
authors.initials: C
authors.lastname: Rodríguez-Pérez
authors.affiliation: Research and Development Functional Food Center (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. rquirantes@ugr.es.
authors.forename: Rosa
authors.initials: R
authors.lastname: Quirantes-Piné
authors.affiliation: Unit of Clinical Pediatrics, San Cecilio University Hospital, 18012 Granada, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Uberos
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain and Research and Development Functional Food Center (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. rquirantes@ugr.es.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Jiménez-Sánchez
authors.affiliation: Microbiology Department, San Cecilio University Hospital, Instituto Biosanitario de Investigación (IBIG), Granada, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Peña
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain and Research and Development Functional Food Center (CIDAF), Health Science Technological Park, Avenida del Conocimiento 37, Edificio BioRegión, 18016 Granada, Spain. rquirantes@ugr.es.
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura-Carretero
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D056689:Uropathogenic Escherichia coli / Q000187:drug effects* / Q000502:physiology; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010636:Phenols; D010936:Plant Extracts