title: Candida albicans β-1,2 mannosyl transferase Bmt3: Preparation and evaluation of a β (1,2), α (1,2)-tetramannosyl fluorescent substrate.
abstract: We describe for the first time the chemical synthesis of a tetramannoside, containing both α (1→2) and β (1→2) linkages. Dodecylthio (lauryl) glycosides were prepared from odorless dodecyl thiol and used as donors for the glycosylation steps. This tetramannoside, was coupled to a mantyl group, and revealed to be a perfect substrate of β-mannosyltransferase Bmt3, confirming the proposed specificity and allowing the preparation of a pentamannoside sequence (β Man (1,2) β Man (1,2) α Man (1,2) α Man (1,2) α Man) usable as a novel substrate for further elongation studies.
authors.affiliation: École Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, 75005 Paris, France; Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005 Paris, France; CNRS, UMR 7203 LBM, F-75005 Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Cattiaux
authors.affiliation: École Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, 75005 Paris, France; Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005 Paris, France; CNRS, UMR 7203 LBM, F-75005 Paris, France.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Mée
authors.affiliation: École Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, 75005 Paris, France; Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005 Paris, France; CNRS, UMR 7203 LBM, F-75005 Paris, France.
authors.forename: Marilyne
authors.initials: M
authors.lastname: Pourcelot
authors.affiliation: Univ. Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Ghenima
authors.initials: G
authors.lastname: Sfihi-Loualia
authors.affiliation: Univ. Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France; Univ. Lille, Inserm, CHU Lille, U995 - LIRIC - Lille Inflammation Research International Center, F-59000 Lille, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hurtaux
authors.affiliation: Univ. Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Maes
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U995 - LIRIC - Lille Inflammation Research International Center, F-59000 Lille, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Fradin
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U995 - LIRIC - Lille Inflammation Research International Center, F-59000 Lille, France.
authors.forename: Boualem
authors.initials: B
authors.lastname: Sendid
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U995 - LIRIC - Lille Inflammation Research International Center, F-59000 Lille, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Poulain
authors.affiliation: Univ. Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Fabre
authors.affiliation: Univ. Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Delplace
authors.affiliation: Univ. Lille, CNRS, UMR 8576, UGSF, Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Guérardel
authors.affiliation: École Normale Supérieure-PSL Research University, Département de Chimie, 24, rue Lhomond, 75005 Paris, France; Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 place Jussieu, F-75005 Paris, France; CNRS, UMR 7203 LBM, F-75005 Paris, France. Electronic address: Jean-Maurice.Mallet@ens.fr.
authors.forename: Jean-Maurice
authors.initials: JM
authors.lastname: Mallet
mesh_terms: D002176:Candida albicans / Q000201:enzymology*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D008362:Mannosides / Q000737:chemistry / Q000378:metabolism*; D008364:Mannosyltransferases / Q000378:metabolism*; D008968:Molecular Conformation; D013379:Substrate Specificity
chemical_list: D005456:Fluorescent Dyes; D008362:Mannosides; D008364:Mannosyltransferases
keywords: Beta mannosides; Fluorescent substrate; Glycosylation; Lauryl glycoside; Mannosyl transferases; Mantyl