title: Identification of Antigenic Glycans from Schistosoma mansoni by Using a Shotgun Egg Glycan Microarray.
abstract: Infection of mammals by the parasitic helminth Schistosoma mansoni induces antibodies to glycan antigens in worms and eggs, but the differential nature of the immune response among infected mammals is poorly understood. To better define these responses, we used a shotgun glycomics approach in which N-glycans from schistosome egg glycoproteins were prepared, derivatized, separated, and used to generate an egg shotgun glycan microarray. This array was interrogated with sera from infected mice, rhesus monkeys, and humans and with glycan-binding proteins and antibodies to gather information about the structures of antigenic glycans, which also were analyzed by mass spectrometry. A major glycan antigen targeted by IgG from different infected species is the FLDNF epitope [Fucα3GalNAcβ4(Fucα3)GlcNAc-R], which is also recognized by the IgG monoclonal antibody F2D2. The FLDNF antigen is expressed by all life stages of the parasite in mammalian hosts, and F2D2 can kill schistosomula in vitro in a complement-dependent manner. Different antisera also recognized other glycan determinants, including core β-xylose and highly fucosylated glycans. Thus, the natural shotgun glycan microarray of schistosome eggs is useful in identifying antigenic glycans and in developing new anti-glycan reagents that may have diagnostic applications and contribute to developing new vaccines against schistosomiasis.
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Megan L
authors.initials: ML
authors.lastname: Mickum
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Nina Salinger
authors.initials: NS
authors.lastname: Prasanphanich
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Natural Sciences, University of Maryland Eastern Shores, Princess Anne, Maryland, USA.
authors.forename: Nelum
authors.initials: N
authors.lastname: Dorabawila
authors.affiliation: Department of Natural Sciences, University of Maryland Eastern Shores, Princess Anne, Maryland, USA.
authors.forename: Msano
authors.initials: M
authors.lastname: Mandalasi
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lasanajak
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.|Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, Atlanta, Georgia, USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Luyai
authors.affiliation: Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, Atlanta, Georgia, USA.
authors.forename: W Evan
authors.initials: WE
authors.lastname: Secor
authors.affiliation: Centers for Disease Control and Prevention, Division of Parasitic Diseases and Malaria, Atlanta, Georgia, USA.
authors.forename: Patricia P
authors.initials: PP
authors.lastname: Wilkins
authors.affiliation: Department of Molecular Cell Biology & Immunology, Glycoimmunology Group, VU University Medical Center, Amsterdam, Netherlands.
authors.forename: Irma
authors.initials: I
authors.lastname: Van Die
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.affiliation: Department of Natural Sciences, University of Maryland Eastern Shores, Princess Anne, Maryland, USA.
authors.forename: A Kwame
authors.initials: AK
authors.lastname: Nyame
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA rcummin1@bidmc.harvard.edu kex9@cdc.gov.|Beth Israel Deaconess Medical Center, Department of Surgery, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
authors.affiliation: Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA rcummin1@bidmc.harvard.edu kex9@cdc.gov.|Centers for Disease Control and Prevention, Division of Select Agents and Toxins, Atlanta, Georgia, USA.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rivera-Marrero
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008253:Macaca mulatta; D013058:Mass Spectrometry; D051379:Mice; D046228:Microarray Analysis; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011485:Protein Binding; D012550:Schistosoma mansoni / Q000276:immunology*; D015053:Zygote / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D007074:Immunoglobulin G; D011134:Polysaccharides