title: Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents.
abstract: Recently it was demonstrated that for urinary tract infections species with a lower or unproven pathogenic potential, such as Delftia tsuruhatensis and Achromobacter xylosoxidans, might interact with conventional pathogenic agents such as Escherichia coli. Here, single- and dual-species biofilms of these microorganisms were characterized in terms of microbial composition over time, the average fitness of E. coli, the spatial organization and the biofilm antimicrobial profile. The results revealed a positive impact of these species on the fitness of E. coli and a greater tolerance to the antibiotic agents. In dual-species biofilms exposed to antibiotics, E. coli was able to dominate the microbial consortia in spite of being the most sensitive strain. This is the first study demonstrating the protective effect of less common species over E. coli under adverse conditions imposed by the use of antibiotic agents.
authors.affiliation: a Laboratory for Process Engineering, Environment, and Energy and Biotechnology Engineering (LEPABE), Faculty of Engineering, Department of Chemical Engineering , University of Porto , Porto , Portugal.|b Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy , University of Southern Denmark , Odense M , Denmark.
authors.forename: Andreia S
authors.initials: AS
authors.lastname: Azevedo
authors.affiliation: a Laboratory for Process Engineering, Environment, and Energy and Biotechnology Engineering (LEPABE), Faculty of Engineering, Department of Chemical Engineering , University of Porto , Porto , Portugal.|c Institute for Biotechnology and Bioengineering (IBB), Centre of Biological Engineering , Universidade do Minho , Braga , Portugal.
authors.forename: Carina
authors.initials: C
authors.lastname: Almeida
authors.affiliation: a Laboratory for Process Engineering, Environment, and Energy and Biotechnology Engineering (LEPABE), Faculty of Engineering, Department of Chemical Engineering , University of Porto , Porto , Portugal.
authors.forename: Bruno
authors.initials: B
authors.lastname: Pereira
authors.affiliation: a Laboratory for Process Engineering, Environment, and Energy and Biotechnology Engineering (LEPABE), Faculty of Engineering, Department of Chemical Engineering , University of Porto , Porto , Portugal.
authors.forename: Luís F
authors.initials: LF
authors.lastname: Melo
authors.affiliation: a Laboratory for Process Engineering, Environment, and Energy and Biotechnology Engineering (LEPABE), Faculty of Engineering, Department of Chemical Engineering , University of Porto , Porto , Portugal.
authors.forename: Nuno F
authors.initials: NF
authors.lastname: Azevedo
mesh_terms: D042441:Achromobacter denitrificans / Q000187:drug effects / Q000502:physiology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D055499:Catheter-Related Infections / Q000188:drug therapy / Q000382:microbiology; D042742:Delftia / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D006801:Humans; D056265:Microbial Interactions / Q000187:drug effects / Q000502:physiology; D062885:Urinary Catheters / Q000009:adverse effects / Q000382:microbiology; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: Escherichia coli; LNA/2′OMe-FISH; Multispecies biofilms; antibiotics; catheter-associated urinary tract infections; uncommon species