title: Bioactive ZnO Coatings Deposited by MAPLE-An Appropriate Strategy to Produce Efficient Anti-Biofilm Surfaces.
abstract: Deposition of bioactive coatings composed of zinc oxide, cyclodextrin and cefepime (ZnO/CD/Cfp) was performed by the Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique. The obtained nanostructures were characterized by X-ray diffraction, IR microscopy and scanning electron microscopy. The efficient release of cefepime was correlated with an increased anti-biofilm activity of ZnO/CD/Cfp composites. In vitro and in vivo tests have revealed a good biocompatibility of ZnO/CD/Cfp coatings, which recommend them as competitive candidates for the development of antimicrobial surfaces with biomedical applications. The release of the fourth generation cephalosporin Cfp in a biologically active form from the ZnO matrix could help preventing the bacterial adhesion and the subsequent colonization and biofilm development on various surfaces, and thus decreasing the risk of biofilm-related infections.
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest,1-7 Polizu Street, Bucharest 011061, Romania. elena_oprea_93@yahoo.co.uk.
authors.forename: Alexandra Elena
authors.initials: AE
authors.lastname: Oprea
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest,1-7 Polizu Street, Bucharest 011061, Romania. loredana.pandel@yahoo.com.
authors.forename: Loredana Mihaela
authors.initials: LM
authors.lastname: Pandel
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest,1-7 Polizu Street, Bucharest 011061, Romania. ana_maria_dumitrescu@yahoo.com.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Dumitrescu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest,1-7 Polizu Street, Bucharest 011061, Romania. ec_andronescu@yahoo.com.
authors.forename: Ecaterina
authors.initials: E
authors.lastname: Andronescu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest,1-7 Polizu Street, Bucharest 011061, Romania. valentina_grumezescu@yahoo.com.|Lasers Department, National Institute for Lasers, Plasma &amp; Materials, P. O. Box MG-36, Magurele 769231, Romania. valentina_grumezescu@yahoo.com.
authors.forename: Valentina
authors.initials: V
authors.lastname: Grumezescu
authors.affiliation: Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, Bucharest 77206, Romania. carmen_balotescu@yahoo.com.|Research Institute of the University of Bucharest, ICUB, Splaiul Independentei 91-95, Bucharest 010271, Romania. carmen_balotescu@yahoo.com.
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.affiliation: Research Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, Petru Rares Street, No. 2, Craiova 200349, Romania. editor@rjme.ro.
authors.forename: Laurenţiu
authors.initials: L
authors.lastname: Mogoantă
authors.affiliation: Research Center for Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova, Petru Rares Street, No. 2, Craiova 200349, Romania. adibalseanu@yahoo.com.
authors.forename: Tudor-Adrian
authors.initials: TA
authors.lastname: Bălşeanu
authors.affiliation: Department of Pharmacognosy &amp; Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Petru Rares Street, No. 2, Craiova 200349, Romania. mogosanu2006@yahoo.com.
authors.forename: George Dan
authors.initials: GD
authors.lastname: Mogoşanu
authors.affiliation: Lasers Department, National Institute for Lasers, Plasma &amp; Materials, P. O. Box MG-36, Magurele 769231, Romania. gabriel.socol@inflpr.ro.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Socol
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest,1-7 Polizu Street, Bucharest 011061, Romania. grumezescu@yahoo.com.
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.affiliation: Flow Cytometry and Cell Therapy Laboratory, Institute of Cellular Biology and Pathology "NicolaeSimionescu" (ICBP), Bucharest 050568, Romania. floriniordache84@yahoo.com.
authors.forename: Florin
authors.initials: F
authors.lastname: Iordache
authors.affiliation: Flow Cytometry and Cell Therapy Laboratory, Institute of Cellular Biology and Pathology "NicolaeSimionescu" (ICBP), Bucharest 050568, Romania. horia.maniu@gmail.com.
authors.forename: Horia
authors.initials: H
authors.lastname: Maniu
authors.affiliation: Department of Electrical &amp; Electronics Engineering, IDEALAB, Koç University, Rumeli Feneri Yolu, Sariyer, Istanbul 34450, Turkey. mchirea@ku.edu.tr.
authors.forename: Mariana
authors.initials: M
authors.lastname: Chirea
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest,1-7 Polizu Street, Bucharest 011061, Romania. alina_m_h@yahoo.com.|Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, Bucharest 77206, Romania. alina_m_h@yahoo.com.|Research Institute of the University of Bucharest, ICUB, Splaiul Independentei 91-95, Bucharest 010271, Romania. alina_m_h@yahoo.com.
authors.forename: Alina Maria
authors.initials: AM
authors.lastname: Holban
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects; D000077723:Cefepime; D002511:Cephalosporins / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D020099:Coated Materials, Biocompatible; D003505:Cyclodextrins; D000077723:Cefepime; D015034:Zinc Oxide
keywords: MAPLE; biofilm inhibition; cyclodextrins; drug delivery; modified surface; zinc oxide