title: Abundance and turnover of GLP-1 producing L-cells in ileal mucosa are not different in patients with and without type 2 diabetes.
abstract: BACKGROUND
  The gastrointestinal hormone GLP-1 is released from enteroendocrine L-cells and augments postprandial insulin secretion. In patients with type 2 diabetes, the incretin effect is markedly diminished. It is unclear, whether this is due to a reduction in the abundance of L-cells in the intestine.
  
  
  METHODS
  Ileal tissue samples from 10 patients with and 10 patients without diabetes that underwent surgery for the removal of colon tumors were included. Tissue sections were stained for GLP-1, Ki67, TUNEL and chromogranin A.
  
  
  RESULTS
  The number of L-cells was not different between patients with and without diabetes in either crypts (1.81±0.21% vs. 1.49±0.24%, respectively; p=0.31) or villi (1.07±0.16% vs. 0.83±0.10%, respectively; p=0.23). L-cell number was higher in crypts than in villi (p<0.0001). L-cell replication was detected rarely and not different between the groups. L-cell apoptosis was similar in patients with and without diabetes in both crypts (7.84±2.77% vs. 8.65±3.77%, p=0.85) and villi (4.48±2.89% vs. 8.62±4.64%, p=0.42). Chromogranin A staining was found in a subset of L-cells only.
  
  
  CONCLUSIONS
  Intestinal L-cell density is higher in crypts than in villi. Chromogranin A is not a prerequisite for GLP-1 production. L-cell density and turnover are not different between patients with and without diabetes. Thus, alterations in the number of GLP-1 producing cells do not explain the reduced incretin effect in patients with type 2 diabetes.
authors.affiliation: Diabetes Division, St. Josef-Hospital, Ruhr-University Bochum, Gudrunstrasse 56, Bochum, 44791, Germany.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Kampmann
authors.affiliation: Diabetes Division, St. Josef-Hospital, Ruhr-University Bochum, Gudrunstrasse 56, Bochum, 44791, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Ueberberg
authors.affiliation: Diabetes Division, St. Josef-Hospital, Ruhr-University Bochum, Gudrunstrasse 56, Bochum, 44791, Germany.
authors.forename: Bjoern A
authors.initials: BA
authors.lastname: Menge
authors.affiliation: Diabetes Division, St. Josef-Hospital, Ruhr-University Bochum, Gudrunstrasse 56, Bochum, 44791, Germany.
authors.forename: Thomas G K
authors.initials: TG
authors.lastname: Breuer
authors.affiliation: Department of Surgery, St. Josef-Hospital, Ruhr-University Bochum, Gudrunstrasse 56, Bochum, 44791, Germany.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Uhl
authors.affiliation: Institute of Pathology, Ruhr-University Bochum, Bürkle de la Camp-Platz 1, Bochum, 44789, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tannapfel
authors.affiliation: Diabetes Division, St. Josef-Hospital, Ruhr-University Bochum, Gudrunstrasse 56, Bochum, 44791, Germany. Electronic address: Juris.meier@rub.de.
authors.forename: Juris J
authors.initials: JJ
authors.lastname: Meier
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D002452:Cell Count; D053379:Chromogranin A / Q000032:analysis / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000378:metabolism*; D005260:Female; D052216:Glucagon-Like Peptide 1 / Q000096:biosynthesis*; D006801:Humans; D007082:Ileum / Q000166:cytology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D019394:Ki-67 Antigen; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor; D053379:Chromogranin A; D019394:Ki-67 Antigen; D052216:Glucagon-Like Peptide 1
keywords: GLP-1; Ki67; L-cell replication; chromogranin A