title: Methylamine irisolidone, a novel compound, increases total ATPase activity and inhibits apoptosis in vivo and in vitro.
abstract: We propose to further research the protective effect of MMI on myocardium ischemic rat model and H9c2 cells that underwent cell apoptosis induced by hypoxia. We established the myocardium ischemic rat model via the cardiac surgical procedures in vivo and treated the model rats with different concentration of MMI. In vitro, with the pretreatment of MMI for 12 h in the model of Na2S2O4-induced hypoxia injury, the H9c2 cells viability was determined by MTT assay. We found that MMI had significantly improved cardiac function of the myocardial ischemia, and significantly decreased the reactive oxygen species level. The expression of P53, Bcl-2, Bax, and caspase-9 was also induced by MMI. In vitro study revealed a concentration-dependent increase in cell viability associated with MMI pretreatment. Annexin V-FITC and PI staining results showed that MMI had a preventive effect on hypoxia-induced apoptosis in H9c2 cells. MMI also inhibited the mitochondrial membrane potential decrease and increased total ATPase activity during hypoxia in H9c2 cells. In conclusion, MMI can enhance the cardiac function in myocardial ischemic rat and increase cell viability and attenuate the apoptosis in H9c2 cells induced by hypoxia, which was associated with inhibiting MMP decreasion and increasing total ATPase activity.
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.|b School of Medicine and Life Sciences , University of Jinan-Shandong Academy of Medical Sciences , Jinan 250200 , China.
authors.forename: Mao-Shan
authors.initials: MS
authors.lastname: Yin
authors.affiliation: c Department of Pharmacology , China Pharmaceutical University , Nanjing 211198 , China.
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Xu
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.
authors.forename: Li
authors.initials: L
authors.lastname: Jie
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.|b School of Medicine and Life Sciences , University of Jinan-Shandong Academy of Medical Sciences , Jinan 250200 , China.
authors.forename: Wu-Mei
authors.initials: WM
authors.lastname: Zheng
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.|b School of Medicine and Life Sciences , University of Jinan-Shandong Academy of Medical Sciences , Jinan 250200 , China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Sun
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.|b School of Medicine and Life Sciences , University of Jinan-Shandong Academy of Medical Sciences , Jinan 250200 , China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.
authors.forename: Fu-Wen
authors.initials: FW
authors.lastname: Wang
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.|b School of Medicine and Life Sciences , University of Jinan-Shandong Academy of Medical Sciences , Jinan 250200 , China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Xi
authors.affiliation: a Key Laboratory for Rare Disease of Shandong Province, Department of Pharmacology , Institute of Materia Medica, Shandong Academy of Medical Sciences , Jinan 250062 , China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Mu
mesh_terms: D000251:Adenosine Triphosphatases / Q000187:drug effects*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053453:Caspase 9 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D015394:Molecular Structure; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C544752:5,7-dihydroxy-6-methoxy-3-(4-methoxyphenyl)-8-((methylamino)methyl)-4H-(1)benzopyran-4-one; D007529:Isoflavones; D017382:Reactive Oxygen Species; D053453:Caspase 9; D000251:Adenosine Triphosphatases
keywords: ATPase; Methylamine irisolidone; apoptosis; mitochondrial membrane potential; myocardial ischemia