title: Chip-based electrochromatography coupled to ESI-MS detection.
abstract: In this study, we present the coupling of chip-based electrochromatography to MS using a glass chip with a monolithically integrated nanoelectrospray emitter. As separation column, an acrylate-based porous polymer monolith is implemented into the glass chip by photopolymerization. For the establishment and development of this method, we used a test mixture detectable with both fluorescence and ESI-MS. After successful evaluation of the approach with the test solutes, it was applied exemplarily for drug analysis such as high-speed separations of benzodiazepines in pharmaceuticals.
authors.affiliation: Institute of Analytical Chemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Dietze
authors.affiliation: Institute of Analytical Chemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Hackl
authors.affiliation: Institute of Analytical Chemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Renata
authors.initials: R
authors.lastname: Gerhardt
authors.affiliation: Institute of Analytical Chemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Seim
authors.affiliation: Institute of Analytical Chemistry, University of Leipzig, Leipzig, Germany.
authors.forename: Detlev
authors.initials: D
authors.lastname: Belder
mesh_terms: D001569:Benzodiazepines / Q000032:analysis; D053801:Capillary Electrochromatography / Q000379:methods*; D005453:Fluorescence; D056656:Lab-On-A-Chip Devices; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
chemical_list: D011108:Polymers; D001569:Benzodiazepines
keywords: Electrochromatography; Mass spectrometry; Microchip