title: Streptococci Engage TLR13 on Myeloid Cells in a Site-Specific Fashion.
abstract: Streptococci are common human colonizers with a species-specific mucocutaneous distribution. At the same time, they are among the most important and most virulent invasive bacterial pathogens. Thus, site-specific cellular innate immunity, which is predominantly executed by resident and invading myeloid cells, has to be adapted with respect to streptococcal sensing, handling, and response. In this article, we show that TLR13 is the critical mouse macrophage (MΦ) receptor in the response to group B Streptococcus, both in bone marrow-derived MΦs and in mature tissue MΦs, such as those residing in the lamina propria of the colon and the dermis, as well as in microglia. In contrast, TLR13 and its chaperone UNC-93B are dispensable for a potent cytokine response of blood monocytes to group B Streptococcus, although monocytes serve as the key progenitors of intestinal and dermal MΦs. Furthermore, a specific role for TLR13 with respect to MΦ function is supported by the response to staphylococci, where TLR13 and UNC-93B limit the cytokine response in bone marrow-derived MΦs and microglia, but not in dermal MΦs. In summary, TLR13 is a critical and site-specific receptor in the single MΦ response to β-hemolytic streptococci.
authors.affiliation: Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, 79106 Freiburg, Germany; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany;
authors.forename: Julia
authors.initials: J
authors.lastname: Kolter
authors.affiliation: Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, 79106 Freiburg, Germany; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany;
authors.forename: Reinhild
authors.initials: R
authors.lastname: Feuerstein
authors.affiliation: Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, 79106 Freiburg, Germany;
authors.forename: Evelyne
authors.initials: E
authors.lastname: Spoeri
authors.affiliation: Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, 79106 Freiburg, Germany; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany;
authors.forename: Kourosh
authors.initials: K
authors.lastname: Gharun
authors.affiliation: Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, 79106 Freiburg, Germany; Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655;
authors.forename: Roland
authors.initials: R
authors.lastname: Elling
authors.affiliation: Institute Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram-Positif, CNRS ERL3526, 75724 Paris Cedex 15, France;
authors.forename: Patrick
authors.initials: P
authors.lastname: Trieu-Cuot
authors.affiliation: Institute of Neuropathology, Medical Center, University of Freiburg, 79106 Freiburg, Germany;
authors.forename: Tobias
authors.initials: T
authors.lastname: Goldmann
authors.affiliation: Regeneration in Hematopoiesis and Animal Models of Hematopoiesis, Faculty of Medicine, Technical University, 01307 Dresden, Germany;
authors.forename: Claudia
authors.initials: C
authors.lastname: Waskow
authors.affiliation: Southwestern Medical School, University of Texas, Dallas, TX 75390;
authors.forename: Zhijian J
authors.initials: ZJ
authors.lastname: Chen
authors.affiliation: Institute of Medical Microbiology, Medical Center, University of Essen, 45147 Essen, Germany;
authors.forename: Carsten J
authors.initials: CJ
authors.lastname: Kirschning
authors.affiliation: Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, 79106 Freiburg, Germany; Center for Sepsis Control and Care, Medical Center, University of Jena, 07747 Jena, Germany; and.
authors.forename: Sachin D
authors.initials: SD
authors.lastname: Deshmukh
authors.affiliation: Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, 79106 Freiburg, Germany; Center for Pediatrics and Adolescent Medicine, Medical Center, University of Freiburg, 79106 Freiburg, Germany philipp.henneke@uniklinik-freiburg.de.
authors.forename: Philipp
authors.initials: P
authors.lastname: Henneke
mesh_terms: D000818:Animals; D003106:Colon / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D006461:Hemolysis; D054884:Host-Pathogen Interactions; D018928:Immunity, Mucosal / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000382:microbiology / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D017628:Microglia / Q000473:pathology; D009928:Organ Specificity; D012867:Skin / Q000473:pathology; D013290:Streptococcal Infections / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000276:immunology*; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*
chemical_list: D016207:Cytokines; D026901:Membrane Transport Proteins; C539093:Tlr13 protein, mouse; D051193:Toll-Like Receptors; C512254:UNC93B1 protein, mouse