title: Identification of proteins derived from Listeria monocytogenes inducing human dendritic cell maturation.
abstract: Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that can promote antitumor immunity when pulsed with tumor antigens and then matured by stimulatory agents. Despite apparent progress in DC-based cancer immunotherapy, some discrepancies were reported in generating potent DCs. Listeria monocytogenes as an intracellular microorganism is able to effectively activate DCs through engaging pattern-recognition receptors (PRRs). This study aimed to find the most potent components derived from L. monocytogenes inducing DC maturation. The preliminary results demonstrated that the ability of protein components is higher than DNA components to promote DC maturation and activation. Protein lysate fractionation demonstrated that fraction 2 HIC (obtained by hydrophobic interaction chromatography) was able to efficiently mature DCs. F2HIC-matured DCs are able to induce allogeneic CD8(+) T cells proliferation better than LPS-matured DCs and induce IFN-γ producing CD8(+) T cells. Mass spectrometry results showed that F2HIC contains 109 proteins. Based on the bioinformatics analysis for these 109 proteins, elongation factor Tu (EF-Tu) could be considered as a PRR ligand for stimulating DC maturation.
authors.affiliation: Immunology Department, School of Medicine, Tehran University of Medical Sciences, Poursina Avenue, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mirzaei
authors.affiliation: Immunology Department, School of Medicine, Tehran University of Medical Sciences, Poursina Avenue, Tehran, Iran.
authors.forename: Azad
authors.initials: A
authors.lastname: Saei
authors.affiliation: Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Torkashvand
authors.affiliation: Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Azarian
authors.affiliation: Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Jalili
authors.affiliation: Immunology Department, School of Medicine, Tehran University of Medical Sciences, Poursina Avenue, Tehran, Iran.
authors.forename: Farshid
authors.initials: F
authors.lastname: Noorbakhsh
authors.affiliation: Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Behrouz
authors.initials: B
authors.lastname: Vaziri
authors.affiliation: Immunology Department, School of Medicine, Tehran University of Medical Sciences, Poursina Avenue, Tehran, Iran. hajatij@sina.tums.ac.ir.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Hadjati
mesh_terms: D001426:Bacterial Proteins / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D008089:Listeria monocytogenes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D010444:Peptide Elongation Factor Tu / Q000276:immunology*; D051192:Receptors, Pattern Recognition / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D051192:Receptors, Pattern Recognition; D010444:Peptide Elongation Factor Tu
keywords: Dendritic cell; Elongation factor Tu; Listeria monocytogenes; Tumor