title: Effect of metal ions on autofluorescence of the dry rot fungus Serpula lacrymans grown on spruce wood.
abstract: This work describes autofluorescence of the mycelium of the dry rot fungus Serpula lacrymans grown on spruce wood blocks impregnated with various metals. Live mycelium, as opposed to dead mycelium, exhibited yellow autofluorescence upon blue excitation, blue fluorescence with ultraviolet (UV) excitation, orange-red and light-blue fluorescence with violet excitation, and red fluorescence with green excitation. Distinctive autofluorescence was observed in the fungal cell wall and in granula localized in the cytoplasm. In dead mycelium, the intensity of autofluorescence decreased and the signal was diffused throughout the cytoplasm. Metal treatment affected both the color and intensity of autofluorescence and also the morphology of the mycelium. The strongest yellow signal was observed with blue excitation in Cd-treated samples, in conjunction with increased branching and the formation of mycelial loops and protrusions. For the first time, we describe pink autofluorescence that was observed in Mn-, Zn-, and Cu-treated samples with UV, violet or. blue excitation. The lowest signals were obtained in Cu- and Fe-treated samples. Chitin, an important part of the fungal cell wall exhibited intensive primary fluorescence with UV, violet, blue, and green excitation.
authors.affiliation: Laboratory of Environmental Microbiology, Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic. gabriel@biomed.cas.cz.
authors.forename: Jiří
authors.initials: J
authors.lastname: Gabriel
authors.affiliation: Laboratory of Characterization of Molecular Structure, Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Žižka
authors.affiliation: Laboratory of Environmental Microbiology, Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Švec
authors.affiliation: Research and Development, Thermo Sanace s.r.o., Chamrádova 475/23, 718 00, Ostrava-Kunčičky, Czech Republic.
authors.forename: Andrea
authors.initials: A
authors.lastname: Nasswettrová
authors.affiliation: Research and Development, Thermo Sanace s.r.o., Chamrádova 475/23, 718 00, Ostrava-Kunčičky, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Šmíra
authors.affiliation: Laboratory of Characterization of Molecular Structure, Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Kofroňová
authors.affiliation: Laboratory of Characterization of Molecular Structure, Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic.
authors.forename: Oldřich
authors.initials: O
authors.lastname: Benada
mesh_terms: D001487:Basidiomycota / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D005453:Fluorescence; D008670:Metals / Q000378:metabolism*; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D028222:Picea / Q000382:microbiology*; D014466:Ultraviolet Rays; D014934:Wood / Q000382:microbiology*
chemical_list: D008670:Metals