title: The spatial distribution of the photostability of thionine in zeolite L nanochannels investigated by Photobleaching Lifetime Imaging Microscopy.
abstract: Dye photobleaching is a photochemical reaction that can be investigated locally using fluorescence microscopy techniques. In this study, a user-friendly computational tool to assist photobleaching experiments called Photobleaching Lifetime Imaging Microscopy (PbLIM) is presented. With this tool it is possible to recover the photobleaching kinetics spatially, where a photobleaching lifetime is generated for each pixel of the image. Our model was applied to the photobleaching process of thionine encapsulated into the one-dimensional nano-channels of Zeolite L (ZL), from where we gained insight into the molecular oxygen distribution inside the ZL channels, as well as the detailed photobleaching of the confined thionine.
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo, 13566-590 São Carlos, SP, Brazil. marcelog@iqsc.usp.br rodrigo_albuquerque@iqsc.usp.br.
authors.forename: Diego
authors.initials: D
authors.lastname: Lencione
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: Gehlen
authors.forename: Loren N
authors.initials: LN
authors.lastname: Trujillo
authors.forename: Renan C F
authors.initials: RC
authors.lastname: Leitao
authors.forename: Rodrigo Q
authors.initials: RQ
authors.lastname: Albuquerque