title: Modulation of fatty acid metabolism and tricarboxylic acid cycle to enhance the lipstatin production through medium engineering in Streptomyces toxytricini.
abstract: This work investigated the potential of medium engineering to obtain maximum biomass, non-conventional carbon sources for lipstatin production and modulation of tricarboxylic acid (TCA) cycle to promote lipstatin synthesis. It was found that 2:3 carbon and nitrogen ratio, produced maximum biomass of 7.9g/L in growth medium and 6.6g/L in pre-seed medium. Among the studied non-conventional carbon sources i.e., soya flour 40g/L and sesame oil 30mL/L were found producing 1109.37mg/L (1.24-fold of control) and 1196.75mg/L (1.34-fold of control) lipstatin respectively. Supplementation of TCA cycle intermediates revealed that NADH and succinic acid showed lipstatin production to 1132.99mg/L and 1171.10mg/L respectively. Experimental outcome was validated in 7L bioreactor and produced 2242.63mg/L lipstatin which was ∼14% higher than shake flask.
authors.affiliation: Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, Maharshi Dayanand University, Rohtak, Haryana, India.
authors.forename: Punit
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, Maharshi Dayanand University, Rohtak, Haryana, India. Electronic address: kashyapdubey@gmail.com.
authors.forename: Kashyap Kumar
authors.initials: KK
authors.lastname: Dubey
mesh_terms: D002952:Citric Acid Cycle; D003470:Culture Media; D005227:Fatty Acids / Q000096:biosynthesis*; D007783:Lactones / Q000378:metabolism*; D050356:Lipid Metabolism; D009243:NAD; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D014815:Vitamins
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D007783:Lactones; D014815:Vitamins; D009243:NAD; C054164:lipstatin
keywords: Acyl CoA carboxylase; Fatty acid metabolism; Lipstatin; Pancreatic lipase inhibitor; Submerged fermentation