title: Neurological function following cerebral ischemia/reperfusion is improved by the Ruyi Zhenbao pill in a rats.
abstract: The present study aimed to investigate the effect and underlying mechanisms of the Ruyi Zhenbao pill on neurological function following cerebral ischemia/reperfusion in rats. Male Sprague-Dawley rats underwent middle cerebral artery occlusion following reperfusion. The rats received intragastrically either sodium carboxymethyl cellulose (control and model groups) or Ruyi Zhenbao pill at doses of 0.2, 0.4 or 0.8 g/kg. Neurological function was assessed by cylinder, adhesive and beam-walking tests after 14-day Ruyi Zhenbao pill treatment. Neurogenesis and angiogenesis were detected using immunoﬂuorescence staining. The expression levels of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assays. Treatment with 0.4 and 0.8 g/kg Ruyi Zhenbao for 14 days signiﬁcantly improved neurological function, and increased the number of von Willebrand Factor- and neuronal nuclear antigen-positive cells in the ischemic hemisphere of rats. Ruyi Zhenbao pill treatment also significantly enhanced the expression levels of BDNF, NGF and VEGF in the ischemic hemisphere. The results demonstrated that the Ruyi Zhenbao pill improved neurological function following ischemia in rats. The mechanisms of the Ruyi Zhenbao pill are associated with increasing the expression levels of BDNF, NGF and VEGF, and subsequently promoting neurogenesis and angiogenesis in the ischemic zone.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Sijin
authors.initials: S
authors.lastname: Duan
authors.affiliation: Shandong ARURA Pharmaceutical R&D Co., Ltd., Jinan, Shandong 250101, P.R. China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Wang
authors.affiliation: Shandong ARURA Pharmaceutical R&D Co., Ltd., Jinan, Shandong 250101, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Food Safety, School of Life Science, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Pharmacology, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Fu
keywords: Ruyi Zhenbao pill; angiogenesis; cerebral ischemia; neurogenesis; neurological function