title: Polymorphisms in DNA Repair Gene XRCC3 and Susceptibility to Breast Cancer in Saudi Females.
abstract: We investigated three common polymorphisms (SNPs) in the XRCC3 gene (rs861539, rs1799794, and rs1799796) in 143 Saudi females suffering from breast cancer (median age = 51.4 years) and 145 age matched normal healthy controls. DNA was extracted from whole blood and genotyping was conducted using PCR-RFLP. rs1799794 showed significant association, where AA and AA+AG occurred at a significantly higher frequency in the cancer patients compared to the control group (OR: 28.1; 95% CI: 3.76-21.12; χ (2): 22.82; p < 0.0001). The G allele was protective and presented with a dominant model. The genotype and allele frequencies of rs861539 C>T and rs1799796 A>G did not show a significant difference when the results in the patients and controls were compared. However, the frequency of rs1799796 differed significantly in patients with different age of diagnosis, tumor grade, and ER and HER2 status. The wild type A allele occurred at a higher frequency in the ER- and HER2- group. Our results among Saudis suggest that some variations in XRCC3 may contribute to breast cancer susceptibility. In conclusion, the results obtained during this study suggest that rs1799794 in XRCC3 shows strong association with breast cancer development in Saudi females.
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Alaa Mohammed
authors.initials: AM
authors.lastname: Ali
authors.affiliation: Comprehensive Cancer Center, Department of Women's Imaging, King Fahad Medical City, Riyadh, Saudi Arabia.
authors.forename: Huda
authors.initials: H
authors.lastname: AbdulKareem
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Al Anazi
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Narasimha
authors.initials: N
authors.lastname: Reddy Parine
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Jilani Purusottapatnam
authors.initials: JP
authors.lastname: Shaik
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Alamri
authors.affiliation: Genome Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Akbar
authors.initials: A
authors.lastname: Ali Khan Pathan
authors.affiliation: Department of Biochemistry, Center for Science and Medical Studies for Girls, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
authors.forename: Arjumand
authors.identifier: 0000-0001-6957-203X
authors.initials: A
authors.lastname: Warsy
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012529:Saudi Arabia
chemical_list: D004268:DNA-Binding Proteins; C110901:X-ray repair cross complementing protein 3