title: Oxygen-binding Protein Fiber and Microgel: Supramolecular Myoglobin-Poly(acrylate) Conjugates.
abstract: A supramolecular conjugate of myoglobin (Mb) and water-soluble poly(acrylate), (PA5k and PA25k , where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme-heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme-propionates is coupled to the side-chain carboxyl groups of poly(acrylate) activated by N-hydroxysuccinimide. The ratios of the heme-modified monomer unit and the unmodified monomer unit (m:n) in the polymer chains of Heme-PA5k and Heme-PA25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb-connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme-modified polymer and dimeric apomyoglobin as a cross-linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan.|Department of Chemistry and Biochemistry, Center for Molecular Systems (CMS), Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.|Japan Science and Technology Agency (JST)-PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Ono
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hisaoka
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan. onoda@chem.eng.osaka-u.ac.jp.
authors.forename: Akira
authors.identifier: http://orcid.org/0000-0002-5791-4386
authors.initials: A
authors.lastname: Onoda
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan.|Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Oohora
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, 565-0871, Japan. thayashi@chem.eng.osaka-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayashi
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D002352:Carrier Proteins / Q000737:chemistry*; D005782:Gels / Q000737:chemistry; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D000179:Acrylates; D002352:Carrier Proteins; D005782:Gels; D009211:Myoglobin; D011108:Polymers; D010100:Oxygen
keywords: biomaterials; conjugation; gels; hemoprotein; microgels; protein-polymer hybrids