title: AtPDCD5 Plays a Role in Programmed Cell Death after UV-B Exposure in Arabidopsis.
abstract: DNA damage responses have evolved to sense and react to DNA damage; the induction of DNA repair mechanisms can lead to genomic restoration or, if the damaged DNA cannot be adequately repaired, to the execution of a cell death program. In this work, we investigated the role of an Arabidopsis (Arabidopsis thaliana) protein, AtPDCD5, which is highly similar to the human PDCD5 protein; it is induced by ultraviolet (UV)-B radiation and participates in programmed cell death in the UV-B DNA damage response. Transgenic plants expressing AtPDCD5 fused to GREEN FLUORESCENT PROTEIN indicate that AtPDCD5 is localized both in the nucleus and the cytosol. By use of pdcd5 mutants, we here demonstrate that these plants have an altered antioxidant metabolism and accumulate higher levels of DNA damage after UV-B exposure, similar to levels in ham1ham2 RNA interference transgenic lines with decreased expression of acetyltransferases from the MYST family. By coimmunoprecipitation and pull-down assays, we provide evidence that AtPDCD5 interacts with HAM proteins, suggesting that both proteins participate in the same pathway of DNA damage responses. Plants overexpressing AtPDCD5 show less DNA damage but more cell death in root tips upon UV-B exposure. Finally, we here show that AtPDCD5 also participates in age-induced programmed cell death. Together, the data presented here demonstrate that AtPDCD5 plays an important role during DNA damage responses induced by UV-B radiation in Arabidopsis and also participates in programmed cell death programs.
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina (M.L.F.F., R.C., L.D., P.C.);Department of Biology, University of Antwerp, Antwerp, 2000 Belgium (H.A., G.T.S.B.); andDepartment of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, 62511 Egypt (H.A.).
authors.forename: María Lorena
authors.initials: ML
authors.lastname: Falcone Ferreyra
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina (M.L.F.F., R.C., L.D., P.C.);Department of Biology, University of Antwerp, Antwerp, 2000 Belgium (H.A., G.T.S.B.); andDepartment of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, 62511 Egypt (H.A.).
authors.forename: Romina
authors.identifier: 0000-0003-1060-1857
authors.initials: R
authors.lastname: Casadevall
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina (M.L.F.F., R.C., L.D., P.C.);Department of Biology, University of Antwerp, Antwerp, 2000 Belgium (H.A., G.T.S.B.); andDepartment of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, 62511 Egypt (H.A.).
authors.forename: Lucio
authors.initials: L
authors.lastname: D'Andrea
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina (M.L.F.F., R.C., L.D., P.C.);Department of Biology, University of Antwerp, Antwerp, 2000 Belgium (H.A., G.T.S.B.); andDepartment of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, 62511 Egypt (H.A.).
authors.forename: Hamada
authors.initials: H
authors.lastname: AbdElgawad
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina (M.L.F.F., R.C., L.D., P.C.);Department of Biology, University of Antwerp, Antwerp, 2000 Belgium (H.A., G.T.S.B.); andDepartment of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, 62511 Egypt (H.A.).
authors.forename: Gerrit T S
authors.identifier: 0000-0001-6014-053X
authors.initials: GT
authors.lastname: Beemster
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina (M.L.F.F., R.C., L.D., P.C.);Department of Biology, University of Antwerp, Antwerp, 2000 Belgium (H.A., G.T.S.B.); andDepartment of Botany, Faculty of Science, University of Beni-Suef, Beni-Suef, 62511 Egypt (H.A.) casati@cefobi-conicet.gov.ar.
authors.forename: Paula
authors.identifier: 0000-0002-3194-4683
authors.initials: P
authors.lastname: Casati
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D009154:Mutation; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D030821:Plants, Genetically Modified; D021381:Protein Transport; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D051017:Apoptosis Regulatory Proteins; D029681:Arabidopsis Proteins; D018744:DNA, Plant; C000615931:PDCD5 protein, Arabidopsis