title: Apigenin Regulates Interleukin-1β-Induced Production of Matrix Metalloproteinase Both in the Knee Joint of Rat and in Primary Cultured Articular Chondrocytes.
abstract: We examined whether apigenin affects the gene expression, secretion and activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the knee joint of rat to evaluate the potential chondroprotective effects of apigenin. Rabbit articular chondrocytes were cultured in a monolayer, and reverse transcription - polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and ADAMTS-5. In rabbit articular chondrocytes, the effects of apigenin on IL-1β-induced secretion and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of apigenin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, apigenin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5. Furthermore, apigenin inhibited the secretion and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that apigenin can regulate the gene expression, secretion, and activity of MMP-3, by directly acting on articular chondrocytes.
authors.affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of Korea.
authors.forename: Jin Sung
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Orthopedic Surgery, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
authors.forename: Dong Kyu
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Orthopedic Surgery, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
authors.forename: Hyun-Dae
authors.initials: HD
authors.lastname: Shin
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of Korea.
authors.forename: Ho Seung
authors.initials: HS
authors.lastname: Jo
authors.affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of Korea.
authors.forename: Jin Hoon
authors.initials: JH
authors.lastname: Jeong
authors.affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of Korea.
authors.forename: Young Lac
authors.initials: YL
authors.lastname: Choi
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Republic of Korea.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
authors.affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of Korea.
authors.forename: Sun-Chul
authors.initials: SC
authors.lastname: Hwang
keywords: Apigenin; Chondrocyte; Metalloproteinase; Osteoarthritis