title: Salinomycin suppresses TGF-β1-induced epithelial-to-mesenchymal transition in MCF-7 human breast cancer cells.
abstract: Epithelial-to-mesenchymal transition (EMT) is the major cause of breast cancer to initiate invasion and metastasis. Salinomycin (Sal) has been found as an effective chemical compound to kill breast cancer stem cells. However, the effect of Sal on invasion and metastasis of breast cancer is unclear. In the present study, we showed that Sal reversed transforming growth factor-β1 (TGF-β1) induced invasion and metastasis accompanied with down-regulation of MMP-2 by experiments on human breast cancer cell line MCF-7. Sal was able to inhibit TGF-β1-induced EMT phenotypic transition and the activation of key signaling molecules involved in Smad (p-Smad2/3,Snail1) and non-Smad (β-catenin, p-p38 MAPK) signals which cooperatively regulate the induction of EMT. Importantly, in a series of breast cancer specimens, we found strong correlation among E-cadherin expression, β-catenin expression, and the lymph node metastatic potential of breast cancer. Our research suggests that Sal is promised to be a chemotherapeutic drug by suppressing the metastasis of breast cancer.
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Mao
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Hou
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Jiazhi
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yan
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian 116044, PR China; The Key Laboratory of Tumor Stem Cell Research of Liaoning Province, Dalian Medical University, Dalian 116044, PR China. Electronic address: lilianhong9177@163.com.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells / Q000187:drug effects; D011714:Pyrans / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D051785:Smad Proteins / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D000095662:Polyether Polyketides
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D011714:Pyrans; D051785:Smad Proteins; D053773:Transforming Growth Factor beta1; C010327:salinomycin; D000095662:Polyether Polyketides
keywords: Breast cancer MCF-7 cells; Epithelial-to-mesenchymal transition; Salinomycin; TGF-β/Non-Smad signaling; TGF-β/Smad signaling