title: Metabolomics analysis in rats after administration of Datura stramonium.
abstract: This study aimed to evaluate the effect of Datura stramonium on rats by examining the differences in urine and serum metabolites between Datura stramonium groups and control group. SIMCA-P+12.0.1.0 software was used for partial least-squares discriminant analysis (PLS-DA) to screen for the differential metabolites. Fifteen metabolites in urine including malonic acid, pentanedioic acid, D-xylose, D-ribose, xylulose, azelaic acid, threitol, glycine, butanoic acid, D-mannose, D-gluconic acid, galactonic acid, myo-inositol, octadecanoic acid, pseudouridine and ten metabolites in serum including alanine, butanedioic acid, L-methionine, propanedioic acid, hexadecanoic acid, D-fructose, tetradecanoic acid, D-glucose, D-galactose, oleic acid were selected as the characteristic metabolites. The PLS-DA scores plot indicated that serum and urine metabolites have a variety of changes among low dose group, high dose group and control group. These metabolites were related with amino metabolism, lipid metabolism and energy metabolism. The result reflected the relationship between metabolites in rat fluid and Datura stramonium spectra. Potential differences in metabolites and metabolic pathway analysis showed that the establishment of urine and serum metabolomics methods for further evaluating drug has great significance.
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University Wenzhou 325000, China.
authors.forename: Shihui
authors.initials: S
authors.lastname: Bao
authors.affiliation: Department of Orthodontics, School and Hospital of Stomatology, Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Feiou
authors.initials: F
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Mengzhi
authors.initials: M
authors.lastname: Xu
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Huang
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University Wenzhou 325035, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: Department of Pharmacy,The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, China.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Pharmacy,The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000, China.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
keywords: Datura stramonium; GC-MS; Metabolomics; PLS-DA