title: TRPM7 channel regulates ox-LDL-induced proliferation and migration of vascular smooth muscle cells via MEK-ERK pathways.
abstract: Transient receptor potential melastatin 7 (TRPM7) plays a key role in the pathophysiological response of multiple cell types. However, the role of TRPM7 channels in ox-LDL-induced proliferation and migration of VSMC remains unclear. This study used the thoracic aorta VSMCs to explore the effects of ox-LDL on cell proliferation and migration and to investigate the underlying molecular mechanisms and signaling pathways. Data demonstrated that ox-LDL significantly increased TRPM7 activity, and induced VSMC proliferation and migration. VSMC proliferation and migration were inhibited by nonspecific TRPM7 blocker 2-APB or synthetic siRNA targeting TRPM7. Furthermore, the phosphorylation of ERK1/2 and MEK1/2 associated with cell proliferation and migration decreased in TRPM7-deficient VSMC. Therefore, TRPM7 may constitute a useful target for the treatment of atherosclerosis.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Jinghan
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ju
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D050197:Atherosclerosis / Q000378:metabolism; D001896:Boron Compounds / Q000494:pharmacology; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D008077:Lipoproteins, LDL / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D050053:TRPM Cation Channels / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001896:Boron Compounds; D008077:Lipoproteins, LDL; D034741:RNA, Small Interfering; D050053:TRPM Cation Channels; C099252:oxidized low density lipoprotein; C109986:2-aminoethoxydiphenyl borate; C486584:Trpm7 protein, rat
keywords: MEK-ERK pathways; TRPM7; VSMCs; migration; proliferation