title: Regulation of Arabidopsis thaliana seed dormancy and germination by 12-oxo-phytodienoic acid.
abstract: We previously demonstrated that the oxylipin 12-oxo-phytodienoic acid (OPDA) acts along with abscisic acid to regulate seed germination in Arabidopsis thaliana, but the mechanistic details of this synergistic interaction remain to be elucidated. Here, we show that OPDA acts through the germination inhibition effects of abscisic acid, the abscisic acid-sensing ABI5 protein, and the gibberellin-sensing RGL2 DELLA protein. We further demonstrate that OPDA also acts through another dormancy-promoting factor, MOTHER-OF-FT-AND-TFL1 (MFT). Both abscisic acid and MFT positively feed back into the OPDA pathway by promoting its accumulation. These results confirm the central role of OPDA in regulating seed dormancy and germination in A. thaliana and underline the complexity of interactions between OPDA and other dormancy-promoting factors such as abscisic acid, RGL2, and MFT.
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Anuja
authors.initials: A
authors.lastname: Dave
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Fabián E
authors.initials: FE
authors.lastname: Vaistij
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom.
authors.forename: Alison D
authors.initials: AD
authors.lastname: Gilday
authors.affiliation: Department of Crop Genetics, John Innes Centre, Norwich NR4 7UH, United Kingdom.
authors.forename: Steven D
authors.identifier: http://orcid.org/0000-0001-6340-1731
authors.initials: SD
authors.lastname: Penfield
authors.affiliation: Centre for Novel Agricultural Products, Department of Biology, University of York, York YO10 5DD, United Kingdom ian.graham@york.ac.uk.
authors.forename: Ian A
authors.identifier: http://orcid.org/0000-0003-4007-1770
authors.initials: IA
authors.lastname: Graham
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D018525:Germination / Q000187:drug effects*; D047908:Intracellular Signaling Peptides and Proteins; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism; D057445:Plant Dormancy / Q000187:drug effects*; D011485:Protein Binding / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D005231:Fatty Acids, Unsaturated; D047908:Intracellular Signaling Peptides and Proteins; C492735:MFT protein, Arabidopsis; D054883:Oxylipins; C025999:12-oxophytodienoic acid; D000040:Abscisic Acid
keywords: Abscisic acid; MFT; OPDA; RGL2.; dormancy; germination