title: Simultaneous recording of brain extracellular glucose, spike and local field potential in real time using an implantable microelectrode array with nano-materials.
abstract: Glucose is the main substrate for neurons in the central nervous system. In order to efficiently characterize the brain glucose mechanism, it is desirable to determine the extracellular glucose dynamics as well as the corresponding neuroelectrical activity in vivo. In the present study, we fabricated an implantable microelectrode array (MEA) probe composed of platinum electrochemical and electrophysiology microelectrodes by standard micro electromechanical system (MEMS) processes. The MEA probe was modified with nano-materials and implanted in a urethane-anesthetized rat for simultaneous recording of striatal extracellular glucose, local field potential (LFP) and spike on the same spatiotemporal scale when the rat was in normoglycemia, hypoglycemia and hyperglycemia. During these dual-mode recordings, we observed that increase of extracellular glucose enhanced the LFP power and spike firing rate, while decrease of glucose had an opposite effect. This dual mode MEA probe is capable of examining specific spatiotemporal relationships between electrical and chemical signaling in the brain, which will contribute significantly to improve our understanding of the neuron physiology.
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, People's Republic of China. University of Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wei
authors.forename: Yilin
authors.initials: Y
authors.lastname: Song
authors.forename: Xinyi
authors.initials: X
authors.lastname: Fan
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Shengwei
authors.initials: S
authors.lastname: Xu
authors.forename: Mixia
authors.initials: M
authors.lastname: Wang
authors.forename: Xinxia
authors.initials: X
authors.lastname: Cai
mesh_terms: D000200:Action Potentials; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D003342:Corpus Striatum / Q000378:metabolism / Q000502:physiology*; D004563:Electrochemistry; D004567:Electrodes, Implanted; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D008297:Male; D064688:Metabolic Flux Analysis / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D008839:Microelectrodes; D036103:Nanotechnology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005947:Glucose