title: Transcripts of genes encoding reproductive neuroendocrine hormones and androgen receptor in the brain and testis of goldfish exposed to vinclozolin, flutamide, testosterone, and their combinations.
abstract: Vinclozolin (VZ) is a pesticide that acts as an anti-androgen to impair reproduction in mammals. However, VZ-induced disruption of reproduction is largely unknown in fish. In the present study, we have established a combination exposure in which adult goldfish were exposed to VZ (30 and 100 μg/L), anti-androgen flutamide (Flu, 300 μg/L), and androgen testosterone (T, 1 μg/L) to better understand effects of VZ on reproductive endocrine system. mRNA levels of kisspeptin (kiss-1 and kiss-2) and its receptor (gpr54), salmon gonadotropin-releasing hormone (gnrh3) and androgen receptor (ar) in the mid-brain, and luteinizing hormone receptor (lhr) in the testis were analyzed and compared with those of control following 10 days of exposure. kiss-1 mRNA level was increased in goldfish exposed to 100 µg/L VZ and to Flu, while kiss-2 mRNA level was increased following exposure to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. gpr54 mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu and 100 µg/L VZ with T. gnrh3 mRNA level was increased in goldfish exposed to 100 µg/L VZ, to Flu, and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. The mid-brain ar mRNA level was increased in goldfish exposed to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. Testicular lhr mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu. These results suggest that VZ and Flu are capable of interfering with kisspeptin and GnRH systems to alter pituitary and testicular horonal functions in adult goldfish and the brain ar mediates VZ-induced disruption of androgen production.
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, 389 25, Vodňany, Czech Republic.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Golshan
authors.affiliation: Department of Biological Sciences, University of Calgary, Calgary, AB, T2N 1N4, Canada.
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Habibi
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, 389 25, Vodňany, Czech Republic. smhalavi1@gmail.com.|Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21, Prague 6, Czech Republic. smhalavi1@gmail.com.
authors.forename: Sayyed Mohammad Hadi
authors.initials: SM
authors.lastname: Alavi
mesh_terms: D000726:Androgen Antagonists / Q000494:pharmacology*; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004347:Drug Interactions; D029941:Fish Proteins / Q000235:genetics; D005485:Flutamide / Q000494:pharmacology*; D005659:Fungicides, Industrial / Q000494:pharmacology; D006054:Goldfish / Q000235:genetics*; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics; D059648:Kisspeptins / Q000235:genetics; D008297:Male; D010080:Oxazoles / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012333:RNA, Messenger / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D011974:Receptors, LH / Q000235:genetics; D012098:Reproduction / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D000726:Androgen Antagonists; D000728:Androgens; D029941:Fish Proteins; D005659:Fungicides, Industrial; D059648:Kisspeptins; D010080:Oxazoles; D012333:RNA, Messenger; D011944:Receptors, Androgen; D043562:Receptors, G-Protein-Coupled; D011974:Receptors, LH; C448022:gonadotropin-releasing hormone-III; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D005485:Flutamide; C025643:vinclozolin; D011761:Pyrrolidonecarboxylic Acid
keywords: Androgen receptor; Flutamide; Kisspeptin; Luteinizing hormone receptor; Salmon gonadotropin-releasing hormone; Testosterone