title: New Silk Fibroin-Based Bioresorbable Microcarriers.
abstract: We fabricated bioresorbable microcarriers from water solution of Bombyx mori silk fi broin. The microcarriers are 3D structures with intricate surface and pores allowing penetration of culture medium, gas exchange, and cell adhesion. Fibroin molecules form hydrophobic structures and normally have a negative charge, which stimulates migration, but inhibits cell adhesion and makes it less effective. In order to improve adhesion efficiency and velocity, gelatin (hydrophilic biopolymer with integrin-recognizing RGD sequence) was added to the microcarrier composition. The resultant bioresorbable microcarriers support adhesion and proliferation of 3T3 murine fibroblasts.
authors.affiliation: Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: A Yu
authors.initials: AY
authors.lastname: Arkhipova
authors.affiliation: Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: M C
authors.initials: MC
authors.lastname: Kotlyarova
authors.affiliation: Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Novichkova
authors.affiliation: V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: O I
authors.initials: OI
authors.lastname: Agapova
authors.affiliation: M. F. Vladimirsky Moscow Regional Research and Clinical Institute, Moscow, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Kulikov
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Kulikov
authors.affiliation: V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Drutskaya
authors.affiliation: V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, Moscow, Russia. igor_agapov@mail.ru.
authors.forename: I I
authors.initials: II
authors.lastname: Agapov
authors.affiliation: Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Moisenovich
mesh_terms: D016475:3T3 Cells; D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D051379:Mice
chemical_list: D001672:Biocompatible Materials; D005780:Gelatin; D005349:Fibroins
keywords: biopolymers; fibroblasts; fibroin; microcarrier