title: Distance Regulated Vesicle Fusion and Docking Mediated by β-Peptide Nucleic Acid SNARE Protein Analogues.
abstract: Artificial SNARE analogues derived from SNARE proteins, which mediate synaptic membrane fusion, are of interest. They mimic the tetrameric α-helix bundle of the SNARE motif with various bio-oligomer recognition units. Interaction between complementary oligomers linked to the respective membrane by lipid or peptide anchors leads to proximity of vesicles and to fusion of lipid bilayers. β-Peptide nucleic acids were introduced as hybrid oligomers with the native SNARE protein transmembrane/linker sequence, in order to evaluate a fusion system that allows distance tuning of approaching membranes. Formation of a four-base pair β-PNA double strand with 20 Å length is sufficient for vesicle membrane fusion. Elongation of the recognition β-PNA duplex in the linker region yielded a 40 Å β-peptide duplex and provided a vesicle-vesicle distance that only supported hemifusion of vesicle membranes.
authors.affiliation: Institut für Organische und Biomolekulare Chemie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077, Göttingen, Germany.
authors.forename: Muheeb
authors.initials: M
authors.lastname: Sadek
authors.affiliation: Institut für Organische und Biomolekulare Chemie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077, Göttingen, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Berndt
authors.affiliation: Abteilung Neurobiologie, Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors.forename: Dragomir
authors.initials: D
authors.lastname: Milovanovic
authors.affiliation: Abteilung Neurobiologie, Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Jahn
authors.affiliation: Institut für Organische und Biomolekulare Chemie, Georg-August-Universität Göttingen, Tammannstrasse 2, 37077, Göttingen, Germany. udieder@gwdg.de.
authors.forename: Ulf
authors.initials: U
authors.lastname: Diederichsen
mesh_terms: D062105:Molecular Docking Simulation; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D050600:SNARE Proteins / Q000737:chemistry*
chemical_list: D020135:Peptide Nucleic Acids; D050600:SNARE Proteins
keywords: SNARE proteins; membranes; protein-protein interactions; transmembrane domains; vesicles; β-peptide