title: Differentiation of human endometrial stem cells into endothelial-like cells on gelatin/chitosan/bioglass nanofibrous scaffolds.
abstract: The capacity of gelatin/chitosan/bioactive glass nanopowders (GEL/CS/BGNPs) scaffolds was investigated for increasing human endometrial stem cells (hEnSCs) differentiation into the endothelial cells in the presence of angiogenic factors. GEL/CS nanofibrous scaffold with different contents of BGNPs were fabricated and assessed. Expression of endothelial markers (CD31, vascular endothelial cadherin (VE-cadherin), and KDR) in differentiated cells was evaluated. Results showed the diameter of nanofiber increases with decreasing the BG content in GEL/CS scaffolds. Moreover, in vitro study indicated that the GEL/CS/BGNPs scaffold with 1.5% BGNPs content provided a suitable three-dimensional structure for endothelial cells differentiation. Thus, the GEL/CS/BGNPs scaffold can be recommended for blood vessels repair.
authors.affiliation: a Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Atefeh
authors.initials: A
authors.lastname: Shamosi
authors.affiliation: b Stem Cell and Transgenic Technology Research Center , Shiraz University of Medical Sciences , Shiraz , Iran.|c Department of Regenerative Medicine , University of Manitoba , Winnipeg , Canada.
authors.forename: Davood
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: a Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Azami
authors.affiliation: a Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Ebrahimi-Barough
authors.affiliation: d Department of Clinical Pathology, Faculty of Veterinary Medicine , University of Tehran , Tehran , Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Siavashi
authors.affiliation: e Department of Medical Nanotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ghanbari
authors.affiliation: a Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Esmaeel
authors.initials: E
authors.lastname: Sharifi
authors.affiliation: a Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Roozafzoon
authors.affiliation: a Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.|f Brain and Spinal Injury Research Center, Imam Khomeini Hospital , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000943:Antigens, Differentiation; C022107:Bioglass; D005780:Gelatin; D048271:Chitosan
keywords: Endothelial cells; gelatin/chitosan/bioglass; human endometrial stem cells; nanofiber scaffold