title: Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis.
abstract: AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Sequence analysis showed that the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain. It belongs to the group A subfamily of bZIP transcription factors. TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots. TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress, and its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis. TaAREB3 protein has transcriptional activation activity, and can bind to the ABRE cis-element in vitro. Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances. TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis. These results demonstrated that TaAREB3 plays an important role in drought and freezing tolerances in Arabidopsis.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Wang
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Mao
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Ruilian
authors.initials: R
authors.lastname: Jing
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003080:Cold Temperature; D035264:Flowers / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000502:physiology; D000072417:Protein Domains; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological / Q000235:genetics*; D014908:Triticum / Q000235:genetics*; D014867:Water / Q000378:metabolism
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D010940:Plant Proteins; D014867:Water
keywords: Abscisic acid; drought tolerance; freezing tolerance; gene expression