title: SGT1 is required in PcINF1/SRC2-1 induced pepper defense response by interacting with SRC2-1.
abstract: PcINF1 was previously found to induce pepper defense response by interacting with SRC2-1, but the underlying mechanism remains uninvestigated. Herein, we describe the involvement of SGT1 in the PcINF1/SRC2-1-induced immunity. SGT1 was observed to be up-regulated by Phytophthora capsici inoculation and synergistically transient overexpression of PcINF1/SRC2-1 in pepper plants. SGT1-silencing compromised HR cell death, blocked H2O2 accumulation, and downregulated HR-associated and hormones-dependent marker genes' expression triggered by PcINF1/SRC2-1 co-overexpression. The interaction between SRC2-1 and SGT1 was found by the yeast two hybrid system and was further confirmed by bimolecular fluorescence complementation and co-immunoprecipitation analyses. The SGT1/SRC2-1 interaction was enhanced by transient overexpression of PcINF1 and Phytophthora capsici inoculation, and SGT1-silencing attenuated PcINF1/SRC2-1 interaction. Additionally, by modulating subcellular localizations of SRC2-1, SGT1, and the interacting complex of SGT1/SRC2-1, it was revealed that exclusive nuclear targeting of the SGT1/SRC2-1 complex blocks immunity triggered by formation of SGT1/SRC2-1, and a translocation of the SGT1/SRC2-1 complex from the plasma membrane and cytoplasm to the nuclei upon the inoculation of P. capsici. Our data demonstrate that the SGT1/SRC2-1 interaction, and its nucleocytoplasmic partitioning, is involved in pepper's immunity against P. capsici, thus providing a molecular link between Ca(2+) signaling associated SRC2-1 and SGT1-mediated defense signaling.
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Zhi-qin
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Lan-ping
authors.initials: LP
authors.lastname: Shi
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Yu
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Rong-zhang
authors.initials: RZ
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Wen
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Ansar
authors.initials: A
authors.lastname: Hussain
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Muhammad Ifnan
authors.initials: MI
authors.lastname: Khan
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Hu
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Cai-ling
authors.initials: CL
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Yang-wen
authors.initials: YW
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Ministry of Education Key Laboratory of Plant Genetic Improvement and Comprehensive Utilization, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Shui-lin
authors.initials: SL
authors.lastname: He
mesh_terms: D021581:Active Transport, Cell Nucleus; D020013:Calcium Signaling; D002212:Capsicum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D016923:Cell Death; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005964:Glucosyltransferases / Q000235:genetics* / Q000276:immunology; D054884:Host-Pathogen Interactions; D010838:Phytophthora / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology; D011485:Protein Binding; D021381:Protein Transport; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins; D011506:Proteins; C085101:elicitin, Phytophthora; D005964:Glucosyltransferases