title: Fyn Plays a Pivotal Role in Fetomaternal Tolerance Through Regulation of Th17 Cells.
abstract: OBJECTIVE
  Spontaneous abortion is a poorly understood phenomenon, although fetomaternal intolerance is known to play an important role in its pathogenesis. The tyrosine-specific phosphotransferase Fyn has been reported as a significant regulator in immune response. However, its role in fetomaternal immune tolerance and contribution to spontaneous abortion remains unclear.
  
  
  METHODS
  Fyn expression was evaluated at the fetomaternal interface of normal pregnant and abortion-prone mice, as well as in decidual tissue obtained from normal human pregnancies and idiopathic miscarriages. A Fyn inhibitor was administrated into the LPS-induced abortion mice to investigate the variation of embryo resorption and local immunity.
  
  
  RESULTS
  Fyn expression fluctuated with the progress of normal pregnancy and was elevated in abortion-prone mice and patients with recurrent spontaneous abortion. The Fyn inhibitor reversed LPS-induced embryo absorption and aberrant inflammatory status including redundant expression of IRF4 and increased proportion of Th17 cells.
  
  
  CONCLUSIONS
  Fyn is confirmed as a negative regulator in fetomaternal immune tolerance, through promoting Th17 cell expansion and proinflammatory factors expression.
authors.affiliation: Center for Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Embryo-Fetal Original Adult Disease, the International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Fu-Ju
authors.initials: FJ
authors.lastname: Tian
authors.affiliation: Center for Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Qing-Zhen
authors.initials: QZ
authors.lastname: Xie
authors.affiliation: Center for Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Center for Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Center for Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000022:Abortion, Spontaneous / Q000276:immunology*; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D003656:Decidua / Q000502:physiology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D050835:Interferon Regulatory Factors / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D051076:Proto-Oncogene Proteins c-fyn / Q000235:genetics / Q000378:metabolism*; D058504:Th17 Cells / Q000276:immunology*; D000099218:Interferon Regulatory Factor-4
chemical_list: D050835:Interferon Regulatory Factors; D051076:Proto-Oncogene Proteins c-fyn; D000099218:Interferon Regulatory Factor-4; C494957:FYN protein, human
keywords: Immunoregulation; T cells; protein tyrosine kinase; spontaneous abortion