title: Inhibition of Tumor Necrosis Factor-α and Interleukin-1β Production in Lipopolysaccharide-Stimulated Monocytes by Methanolic Extract of Elephantopus scaber Linn and Identification of Bioactive Components.
abstract: The methanolic extract of E. scaber Linn was evaluated for anti-inflammatory activity by determining its effects on production of pro-inflammatory cytokines like Tumor necrosis factor-α (TNF-α) and Interleukin-1β (IL-1β) in Lipopolysaccharide (LPS) stimulated monocytes. The cytotoxicity of the extract was analyzed prior to the cytokine quantification assays. The extract was further subjected to UPLC MS Q-TOF, for the identification of bioactive components present in the crude extract. The extract was found not to be cytotoxic against monocytes, and exhibited significant inhibition in the production of pro-inflammatory cytokines. The presence of 34 components in the methanolic extract was detected through mass spectrum analysis.
authors.affiliation: Inflammation Research Lab, School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India, 686560.
authors.forename: Anu P
authors.initials: AP
authors.lastname: Abhimannue
authors.affiliation: Inflammation Research Lab, School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India, 686560.
authors.forename: Mohind C
authors.initials: MC
authors.lastname: Mohan
authors.affiliation: Inflammation Research Lab, School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India, 686560. prakashkumar@mgu.ac.in.
authors.forename: Prakash Kumar
authors.initials: PK
authors.lastname: B
mesh_terms: D019659:Asteraceae / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000737:chemistry; D009000:Monocytes / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha
keywords: Elephantopus scaber; Inflammation; Interleukin-1β; Mass spectrometry; Monocytes; Pro-inflammatory cytokines; Tumor necrosis factor-α