title: Biocompatibility of artificial bone based on vancomycin loaded mesoporous silica nanoparticles and calcium sulfate composites.
abstract: The aim of this study was to evaluate the in vitro and in vivo biocompatibility of artificial bone based on vancomycin loaded mesoporous silica nanoparticles and calcium sulfate composites. In vitro cytotoxicity tests by cholecystokinin octapeptide (CCK-8) assay showed that the 5%Van-MSN-CaSO4 and Van-CaSO4 bone cements were cytocompatible for mouse osteoblastic cell line MC3T3-E1. The microscopic observation confirmed that MC3T3-E1cells incubated with Van-CaSO4 group and 5%Van-MSN-CaSO4 group exhibited clear spindle-shaped changes, volume increase and maturation, showing that these cements supported adhesion of osteoblastic cells on their surfaces. In addition, the measurement of alkaline phosphatase activity revealed the osteoconductive property of these biomaterials. In order to assess in vivo biocompatibility, synthesized cements were implanted into the distal femur of twelve adult male and female New Zealand rabbits. After implantation in artificial defects of the distal femur, 5%Van-MSN-CaSO4 and Van-CaSO4 bone cements did not damage the function of main organs of rabbits. In addition, the Van-MSN-CaSO4 composite allowed complete repair of bone defects with new bone formation 3 months after implantation. These results show potential application of Van-MSN-CaSO4 composites as bone graft materials for the treatment of open fracture in human due to its mechanical, osteoconductive and potential sustained drug release characteristics and the absence of adverse effects on the body.
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Yanchang Road 301, Shanghai, 200072, China.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Gu
authors.affiliation: The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, 210029, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Yanchang Road 301, Shanghai, 200072, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Fan
authors.affiliation: The First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu, 210029, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Changhai Hospital, Second Military Medical University, Changhai Road 168, Shanghai, 200433, China. huweicj@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Yanchang Road 301, Shanghai, 200072, China. caixbjn@163.com.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Cai
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001843:Bone Cements / Q000009:adverse effects* / Q000737:chemistry; D016025:Bone Transplantation; D002133:Calcium Sulfate / Q000009:adverse effects* / Q000737:chemistry; D049109:Cell Proliferation; D002470:Cell Survival; D005260:Female; D008297:Male; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles / Q000009:adverse effects* / Q000737:chemistry; D011817:Rabbits; D012822:Silicon Dioxide / Q000009:adverse effects* / Q000737:chemistry; D014640:Vancomycin / Q000009:adverse effects* / Q000737:chemistry
chemical_list: D001843:Bone Cements; D014640:Vancomycin; D012822:Silicon Dioxide; D000469:Alkaline Phosphatase; D002133:Calcium Sulfate