title: Detection of Multiple Budding Yeast Cells and a Partial Sequence of 43-kDa Glycoprotein Coding Gene of Paracoccidioides brasiliensis from a Case of Lacaziosis in a Female Pacific White-Sided Dolphin (Lagenorhynchus obliquidens).
abstract: Lacaziosis, formerly called as lobomycosis, is a zoonotic mycosis, caused by Lacazia loboi, found in humans and dolphins, and is endemic in the countries on the Atlantic Ocean, Indian Ocean and Pacific Ocean of Japanese coast. Susceptible Cetacean species include the bottlenose dolphin (Tursiops truncatus), the Indian Ocean bottlenose dolphin (T. aduncus), and the estuarine dolphin (Sotalia guianensis); however, no cases have been recorded in other Cetacean species. We diagnosed a case of Lacaziosis in a Pacific white-sided dolphin (Lagenorhynchus obliquidens) nursing in an aquarium in Japan. The dolphin was a female estimated to be more than 14 years old at the end of June 2015 and was captured in a coast of Japan Sea in 2001. Multiple, lobose, and solid granulomatous lesions with or without ulcers appeared on her jaw, back, flipper and fluke skin, in July 2014. The granulomatous skin lesions from the present case were similar to those of our previous cases. Multiple budding and chains of round yeast cells were detected in the biopsied samples. The partial sequence of 43-kDa glycoprotein coding gene confirmed by a nested PCR and sequencing, which revealed a different genotype from both Amazonian and Japanese lacaziosis in bottlenose dolphins, and was 99 % identical to those derived from Paracoccidioides brasiliensis; a sister fungal species to L. loboi. This is the first case of lacaziosis in Pacific white-sided dolphin.
authors.affiliation: Laboratory of Aquatic Medicine, School of Veterinary Medicine, Nippon Veterinary and Life Science University, Kyonan-Cho 1-7-1, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Minakawa
authors.affiliation: Okinawa Churashima Foundation, Aza Ishikawa 888, Motobu-Cho, Kunigami-Gun, Okinawa, 905-0206, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ueda
authors.affiliation: Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinkyu-Campus, Osaka Prefecture University, Rinkyu Ohrai Kita 1-58, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Miyuu
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinkyu-Campus, Osaka Prefecture University, Rinkyu Ohrai Kita 1-58, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinkyu-Campus, Osaka Prefecture University, Rinkyu Ohrai Kita 1-58, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kuwamura
authors.affiliation: Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinkyu-Campus, Osaka Prefecture University, Rinkyu Ohrai Kita 1-58, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Izawa
authors.affiliation: Department of Agro-Environmental Sciences, Faculty of Agriculture, University of the Ryukyus, Sembaru 1, Nishihara-Cho, Nakagusuku-Gun, Okinawa, 903-0213, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Konno
authors.affiliation: Laboratory of Veterinary Pathology, Division of Veterinary Sciences, Rinkyu-Campus, Osaka Prefecture University, Rinkyu Ohrai Kita 1-58, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Jyoji
authors.initials: J
authors.lastname: Yamate
authors.affiliation: Department of Pathological Science, CCB, State University of Londrina, P.O. Box 6001, Londrina, PR, 86051-970, Brazil.
authors.forename: Eiko Nakagawa
authors.initials: EN
authors.lastname: Itano
authors.affiliation: Department of Animal Sciences, Faculty of Agriculture, University of the Ryukyus, Sembaru 1, Nishihara-Cho, Nakagusuku-Gun, Okinawa, 903-0213, Japan. aya_grimalkin@yahoo.co.jp.
authors.forename: Ayako
authors.initials: A
authors.lastname: Sano
authors.affiliation: Laboratory of Aquatic Medicine, School of Veterinary Medicine, Nippon Veterinary and Life Science University, Kyonan-Cho 1-7-1, Musashino, Tokyo, 180-8602, Japan.
authors.forename: Shinpei
authors.initials: S
authors.lastname: Wada
mesh_terms: D000818:Animals; D000836:Animals, Zoo; D000946:Antigens, Fungal / Q000235:genetics*; D001706:Biopsy; D004289:Dolphins; D005260:Female; D005656:Fungal Proteins / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D006651:Histocytochemistry; D007564:Japan; D007568:Jaw / Q000473:pathology; D060367:Lacazia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D060368:Lobomycosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008168:Lung / Q000000981:diagnostic imaging / Q000473:pathology; D008853:Microscopy; D016133:Polymerase Chain Reaction; D013902:Radiography, Thoracic; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012867:Skin / Q000473:pathology
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins
keywords: Lacazia loboi; Lacaziosis; Lagenorhynchus obliquiden; Pacific white-sided dolphin; Paracoccidioides brasiliensis