title: Role of apoptosis-related miRNAs in resveratrol-induced breast cancer cell death.
abstract: Breast cancer is the most frequently diagnosed cancer in women, and one of the leading causes of cancer-related deaths worldwide. Recent evidences indicate that dietary agents such as resveratrol may inhibit cancer progression through modulation of microRNAs (miRNAs). We demonstrate that resveratrol regulates apoptotic and cell cycle machinery in breast cancer cells by modulating key tumor-suppressive miRNAs including miR-125b-5p, miR-200c-3p, miR-409-3p, miR-122-5p and miR-542-3p. Resveratrol-mediated miRNA modulation regulates key anti-apoptotic and cell cycle proteins including Bcl-2, X-linked inhibitor of apoptosis protein and CDKs, which are critical for its activity. Modulating miRNAs with mimics or inhibitors further validated a key role for miR-542-3p in MCF-7 and miR-122-5p in MDA-MB-231 breast cancer cell death in response to resveratrol. In conclusion, this study reveals novel miRNAs modulated by resveratrol that have a key role in breast cancer cell death.
authors.affiliation: Department of Pharmaceutical Sciences, Hampton University, Hampton, VA, USA.
authors.forename: R
authors.initials: R
authors.lastname: Venkatadri
authors.affiliation: Department of Pharmaceutical Sciences, Hampton University, Hampton, VA, USA.
authors.forename: T
authors.initials: T
authors.lastname: Muni
authors.affiliation: Department of Pharmaceutical Sciences, Hampton University, Hampton, VA, USA.
authors.forename: A K V
authors.initials: AK
authors.lastname: Iyer
authors.affiliation: Department of Pharmaceutical Sciences, Hampton University, Hampton, VA, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Yakisich
authors.affiliation: Department of Pharmaceutical Sciences, Hampton University, Hampton, VA, USA.
authors.forename: N
authors.initials: N
authors.lastname: Azad
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D035683:MicroRNAs; D019253:Proto-Oncogene Proteins c-bcl-2; D013267:Stilbenes; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D020169:Caspases; D000077185:Resveratrol