title: Assessment and prognostic analysis of EGFR mutations or/and HER2 overexpression in Uygur's Non-small Cell Lung Cancer.
abstract: OBJECTIVE
  The epidermal growth factor receptor (EGFR) mutations and human epidermal growth factor receptor HER-2/neu (HER2) have been established roles in the signal transduction pathways leading to cell growth and differentiation. The present study focus on the significance of EGFR mutations combined with HER2 overexpression on survival outcomes in Non-small Cell Lung Cancer patients in Uygur population.
  
  
  METHODS
  A total of 111 consecutive Uygurods: A total of 111 consecutive Cell Lung Cancer under went lung Cell Lung biopsy or surgery at the Affiliated Tumor Hospital of Xin Jiang Medical University between March 2009 and January 2013 were included in this retrospective study. All the patients included had received gefitinib 250 mg once daily. The HER2 expression were evaluated by immunohistochemical staining with score of membranous staining being 0 = none, 1 = weak, 2 = 10-30% cells, 3≥30% cells stained, and Real-time PCR techniques were conducted to detect mutations of EGFR through 21 kinds of human EGFR gene mutation detection kits. A retrospective review of the medical records was analyzed to determine the correlation between the presence of EGFR mutations combined with HER2 overexpression and clinicopathological factors.
  
  
  RESULTS
  The overall rate of EGFR mutation was 10.81% (n = 12), which mainly involved exons 19 (83.33%, n = 10), 21 (16.67%, n = 2). The overall rate of HER2 overexpression was 21.62% (n = 24). EGFR mutation combined with HER2 overexpression analysis was performed in 111 patients, with an overall rate of 5.41% (n = 6). Median progression-free survival and overall survival were significantly longer in the EGFR mutations group than in the wild type group (PFS: 10.0±1.5 versus 3.8±1.4 months, P = 0.000; OS: 27.3±2.9 versus 19.1±4.7 months, P = 0.000). The ORR in patients with HER2 overexpression was 29.17%, and 13.80% in those patients with HER2 negative, but no significant difference (P = 0.121). The median PFS and OS in HER2 positive group showed no significant difference compared with HER2 negative group (PFS: 4.7±1.2 months versus 3.9±1.6 months, P = 0.085; OS: 20.5±2.4 versus 19.2±2.6 months, P = 0.094). As regarding to ORR, PFS and OS, EGFR mutations combined with HER2 overexpression patients showed no superior efficacy to gefitinib treatment compared with EGFR mutations combined with HER2 negative.
  
  
  CONCLUSIONS
  In Uygur population, progression-free survivals were improved in Non-small Cell Lung Cancer with EGFR mutations. HER2 overexpression provided a poor prognostic factor in Non-small Cell Lung Cancer.
authors.affiliation: Department of Oncology, The Sixth Division Hospital, Xinjiang Production and Construction Corps Wujiaqu, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University Urumqi, China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Du
authors.affiliation: Department of Endocrinology, The Eastern Section of Linyi People's Hospital Linyi, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Central Laboratory, The First Affiliated Hospital of Xinjiang Medical University Urumqi, China.
authors.forename: Jianling
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department of Internal Medicine, The Affiliated Tumor Hospital of Xinjiang Medical University Urumqi, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Xinjiang Medical University Urumqi, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Jia
authors.affiliation: Xinjiang Medical University Urumqi, China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Medicine, The Affiliated Tumor Hospital of Xinjiang Medical University Urumqi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Shan
keywords: HER2 overexpression; Non-small cell lung cancer; Uygur population; epidermal growth factor receptor mutation; gefitinib