title: Effect of a Metalloantibiotic Produced by Pseudomonas aeruginosa on Klebsiella pneumoniae Carbapenemase (KPC)-producing K. pneumoniae.
abstract: Multidrug-resistant organisms (MDRO) are a great problem in hospitals, where thousands of people are infected daily, with the occurrence of high mortality rates, especially in infections caused by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-producing Kpn). The challenge is to find new compounds that can control KPC producing-Kpn infections. The aim of this study was to evaluate the antibiotic activity of the F3d fraction produced by the Pseudomonas aeruginosa LV strain against clinical isolates of KPC-producing Kpn. The results showed that the minimum inhibitory concentration of F3d (62.5 µg mL(-1)), containing an organic metallic compound, killed planktonic cells of KPC-producing Kpn strains after 30 min of incubation. At the same concentration, this fraction also showed an inhibitory effect against biofilm of these bacteria after 24 h of incubation. Treatment with the F3d fraction caused pronounced morphological alterations in both planktonic and biofilm cells of the bacteria. The inhibitory effect of the F3d fraction seems to be more selective for the bacteria than the host cells, indicating its potential in the development of new drugs for the treatment of infections caused by KPC-producing Kpn and other MDRO.
authors.forename: Gilselena
authors.initials: G
authors.lastname: Kerbauy
authors.forename: Ana C P
authors.initials: AC
authors.lastname: Vivan
authors.forename: Glenda C
authors.initials: GC
authors.lastname: Simões
authors.forename: Ane S
authors.initials: AS
authors.lastname: Simionato
authors.forename: Marsileni
authors.initials: M
authors.lastname: Pelisson
authors.forename: Eliana C
authors.initials: EC
authors.lastname: Vespero
authors.forename: Silvia F
authors.initials: SF
authors.lastname: Costa
authors.forename: Celia G T de J
authors.initials: CG
authors.lastname: Andrade
authors.forename: Daiane M
authors.initials: DM
authors.lastname: Barbieri
authors.forename: João C P
authors.initials: JC
authors.lastname: Mello
authors.forename: Alexandre T
authors.initials: AT
authors.lastname: Morey
authors.forename: Lucy M
authors.initials: LM
authors.lastname: Yamauchi
authors.forename: Sueli F
authors.initials: SF
authors.lastname: Yamada-Ogatta
authors.forename: Admilton G
authors.initials: AG
authors.lastname: de Oliveira
authors.affiliation: Department of Microbiology, Londrina State University, CEP: 86051-990, Londrina, Brazil. andradeg@uel.br.
authors.forename: Galdino
authors.initials: G
authors.lastname: Andrade
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects; D007711:Klebsiella pneumoniae / Q000187:drug effects*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase