title: Metabolizer in vivo of fullerenes and metallofullerenes by positron emission tomography.
abstract: Fullerenes (C60) and metallofullerenes (Gd@C82) have similar chemical structure, but the bio-effects of both fullerene-based materials are distinct in vivo. Tracking organic carbon-based materials such as C60 and Gd@C82 is difficult in vivo due to the high content of carbon element in the living tissues themselves. In this study, the biodistribution and metabolism of fullerenes (C60 and Gd@C82) radiolabeled with (64)Cu were observed by positron emission tomography (PET). (64)Cu-C60 and (64)Cu-Gd@C82 were prepared using 1, 4, 7, 10-tetrakis (carbamoylmethyl)-1, 4, 7, 10-tetra-azacyclodo-decanes grafted on carbon cages as a chelator for (64)Cu, and were obtained rapidly with high radiochemical yield (≥90%). The new radio-conjugates were evaluated in vivo in the normal mouse model and tissue distribution by small animal PET/CT imaging and histology was carried out. The PET imaging, the biodistribution and the excretion of C60 and Gd@C82 indicated that C60 samples have higher blood retention and lower renal clearance than the Gd@C82 samples in vivo and suggested that the differences in metabolism and distribution in vivo were caused by the structural differences of the groups on the fullerene cages though there is chemical similarity between C60 and Gd@C82.
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterial & Nanosafety, Institute of High Energy Physics, Chinese Academy of Science (CAS), Beijing 100049, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Wenjiang
authors.initials: W
authors.lastname: Yang
authors.forename: Rongli
authors.initials: R
authors.lastname: Cui
authors.forename: Dongliang
authors.initials: D
authors.lastname: Wang
authors.forename: Yanan
authors.initials: Y
authors.lastname: Chang
authors.forename: Weihong
authors.initials: W
authors.lastname: Gu
authors.forename: Wenyan
authors.initials: W
authors.lastname: Yin
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.forename: Kui
authors.initials: K
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Xia
authors.forename: Huan
authors.initials: H
authors.lastname: Geng
authors.forename: Gengmei
authors.initials: G
authors.lastname: Xing