title: Negatively-charged residues in the polar carboxy-terminal region in FSP27 are indispensable for expanding lipid droplets.
abstract: FSP27 has an important role in large lipid droplet (LD) formation because it exchanges lipids at the contact site between LDs. In the present study, we clarify that the amino-terminal domain of FSP27 (amino acids 1-130) is dispensable for LD enlargement, although it accelerates LD growth. LD expansion depends on the carboxy-terminal domain of FSP27 (amino acids 131-239). Especially, the negative charge of the acidic residues (D215, E218, E219 and E220) in the polar carboxy-terminal region (amino acids 202-239) is essential for the enlargement of LD. We propose that the carboxy-terminal domain of FSP27 has a crucial role in LD expansion, whereas the amino-terminal domain only has a supportive role.
authors.affiliation: Division of Diabetes and Endocrinology, Department of Internal Medicine, Chibune General Hospital, Osaka, Japan.|Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tamori
authors.affiliation: Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.|Division of Diabetes, Department of Internal Medicine, Kakogawa West City Hospital, Japan.
authors.forename: Sanshiro
authors.initials: S
authors.lastname: Tateya
authors.affiliation: Division of Biochemistry, Department of Molecular and Cellular Biology, Kobe University Graduate School of Medicine, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ijuin
authors.affiliation: Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nishimoto
authors.affiliation: Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Nakajima
authors.affiliation: Division of Diabetes and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Ogawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D050356:Lipid Metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016296:Mutagenesis; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity
chemical_list: D050505:Mutant Proteins; D010446:Peptide Fragments; D011506:Proteins; D011994:Recombinant Proteins; C525805:fat-specific protein 27, mouse
keywords: adipocyte; fat specific protein of 27 kDa; lipid droplet; lipid droplet enlargement; negatively-charged amino acid; structure and function