title: Bone marrow donor-related variables associated with harvest outcome in HLA-haploidentical transplantation with postinfusion cyclophosphamide.
abstract: OBJECTIVE
  Several transplantation outcomes have been shown to be associated with the infused bone marrow cell dose/kg of the recipient's body weight. The donor bone marrow density is directly related to the infused cell dose. The aim of the present study was to identify donor-related variables that are associated with high donor bone marrow density.
  
  
  METHODS
  We retrospectively analysed the predictive factors of high marrow density in 65 consecutive HLA-haploidentical bone marrow donors harvested at our centre between 2009 and 2013.
  
  
  RESULTS
  Body mass index (BMI) and peripheral white blood cell (WBC) count were directly associated with bone marrow density (regression coefficient β = 5·33 and β = 2·93, respectively; P < 0·01). The likelihood of obtaining a collection with a high density was first predicted using BMI (BMI ≥30, mean density = 25·8 TNC/ml × 10(6) ). Second, donors with a BMI <30 were split into two groups according to peripheral WBC count (WBC <8 × 10(3) /mm(3) : mean density = 18·4 TNC/ml × 10(6) ; WBC ≥8 × 10(3) /mm(3) : mean density = 23·1 TNC/ml × 10(6) ). We also observed that the density of the first collected bag directly correlated with the overall density (R(2) = 0·69, P < 0·01).
  
  
  CONCLUSIONS
  The donor-related features BMI and WBC count affect the cell quantity obtainable with the harvest and should be taken into account when choosing the donor.
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: A
authors.identifier: http://orcid.org/0000-0002-5183-9696
authors.initials: A
authors.lastname: Rimondo
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: S
authors.initials: S
authors.lastname: Bramanti
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: R
authors.initials: R
authors.lastname: Crocchiolo
authors.affiliation: Biostatistics Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: L
authors.initials: L
authors.lastname: Giordano
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: B
authors.initials: B
authors.lastname: Sarina
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: L
authors.initials: L
authors.lastname: Morabito
authors.affiliation: Immunohaematology and Transfusion Service, IRCCS Policlinico San Matteo Foundation, Pavia, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Perotti
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: I
authors.initials: I
authors.lastname: Timofeeva
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: R
authors.initials: R
authors.lastname: Capizzuto
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: A
authors.initials: A
authors.lastname: Santoro
authors.affiliation: Hematology Unit, Humanitas Cancer Center, Rozzano (Milan), Italy.
authors.forename: L
authors.initials: L
authors.lastname: Castagna
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D001782:Blood Donors; D015992:Body Mass Index; D001835:Body Weight / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007902:Length of Stay; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D003520:Cyclophosphamide
keywords: bone marrow density; bone marrow donors; donor selection; haploidentical transplantation; predictive collection tree