title: Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification.
abstract: Next-generation sequencing provides large-scale sequencing data with relative ease and at a reasonable cost, making it possible to identify a large amount of SSR markers in a timely and cost-effective manner. On the basis of the transcriptome database of Sinonovacula constricta obtained by Illumina/Solexa pyrosequencing, 60 polymorphic SSR markers were developed and characterized in 30 individuals. The number of alleles per polymorphic locus ranged from 2 to 7 with an average of 3.75 alleles. The observed and expected heterozygosities varied from 0.050 to 1.000 and from 0.050 to 0.836, respectively. Nineteen loci significantly deviated from Hardy-Weinberg equilibrium (P < 0.01) after Bonferroni's correction for multiple tests. In addition, interspecific transferability revealed that 20 polymorphic loci in Solen linearis were first characterized in this study. To the best of our knowledge, this is the highest number of SSRs in S. constricta and the first report of cross-species amplification. These novel polymorphic SSR markers will be particularly useful for conservation genetics, evolutionary studies, genetic trait mapping, and marker assisted selection in the species.
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences.
authors.forename: Y H
authors.initials: YH
authors.lastname: Dong
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences.
authors.forename: H H
authors.initials: HH
authors.lastname: Yao
authors.affiliation: College of Life Sciences, Taizhou University, Linhai, China.
authors.forename: C S
authors.initials: CS
authors.lastname: Sun
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences.
authors.forename: D M
authors.initials: DM
authors.lastname: Lv
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Li
authors.affiliation: Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Lin
mesh_terms: D000483:Alleles; D000818:Animals; D049872:Bivalvia / Q000145:classification / Q000235:genetics*; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D056426:Genetic Loci; D005819:Genetic Markers; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D059467:Transcriptome
chemical_list: D005819:Genetic Markers