title: Antioxidant and Physicochemical Properties of Hydrogen Peroxide-Treated Sugar Beet Dietary Fibre.
abstract: The aim of the present work was to examine if hydrogen peroxide treatment of sugar beet fibre that aimed at improving its physicochemical properties would impair its antioxidant potential. Three different sugar beet fibres were obtained from sugar beet - non-treated fibre (NTF) from sugar beet cossettes extracted with sulphurous acid, treated fibre (TF) from NTF treated with hydrogen peroxide in alkaline solution and commercially available Fibrex(®) . The antioxidant activity of extractable and non-extractable fibre fractions in ethanol/water mixture (80:20, v/v) of three fibre samples was estimated. Non-extractable fractions obtained after alkaline treatment of investigated fibres were much higher in phenolic compounds and possessed higher antioxidant potential than extractable fractions. Ferulic acid was proven to be the dominant phenolic acid. Regarding both extractable and non-extractable fractions, Fibrex(®) had the highest antioxidant activity in chemical tests, while NTF was superior in comparison with TF. Based on the results of Caco-2 cells-based test, all non-extractable fractions possessed potential for reactive oxygen species inhibition. Regarding the extractable fractions, only the TF manifested this effect.Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad, 21000, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mišan
authors.affiliation: Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad, 21000, Serbia.
authors.forename: Marijana
authors.initials: M
authors.lastname: Sakač
authors.affiliation: Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad, 21000, Serbia.
authors.forename: Đorđe
authors.initials: Đ
authors.lastname: Medić
authors.affiliation: Institute for Medicinal Plant Research 'Dr Josif Pančić', Tadeuša Košćuška 1, Belgrade, 11000, Serbia.
authors.forename: Vanja
authors.initials: V
authors.lastname: Tadić
authors.affiliation: Institute for Medicinal Plant Research 'Dr Josif Pančić', Tadeuša Košćuška 1, Belgrade, 11000, Serbia.
authors.forename: Goran
authors.initials: G
authors.lastname: Marković
authors.affiliation: Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad, 21000, Serbia.
authors.forename: Julliana
authors.initials: J
authors.lastname: Gyura
authors.affiliation: Department of Pharmacy, University of Naples Federico II, via D. Montesano 49, Naples, 80131, Italy.
authors.forename: Ester
authors.initials: E
authors.lastname: Pagano
authors.affiliation: Department of Pharmacy, University of Naples Federico II, via D. Montesano 49, Naples, 80131, Italy.
authors.forename: Angelo A
authors.initials: AA
authors.lastname: Izzo
authors.affiliation: Department of Pharmacy, University of Naples Federico II, via D. Montesano 49, Naples, 80131, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Borrelli
authors.affiliation: Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad, 21000, Serbia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Šarić
authors.affiliation: Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad, 21000, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Milovanović
authors.affiliation: Faculty of Medicine, University of Novi Sad, Veljka Vlahovića 3, Novi Sad, 21000, Serbia.
authors.forename: Nataša
authors.initials: N
authors.lastname: Milić
mesh_terms: D000975:Antioxidants; D027461:Beta vulgaris / Q000737:chemistry*; D004043:Dietary Fiber / Q000032:analysis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004043:Dietary Fiber; D006861:Hydrogen Peroxide
keywords: Caco-2 cell assay; Fibrex®; antioxidant activity; non-treated fibre; sugar beet fibre; treated fibre