title: A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility.
abstract: The surface expression of CD9 (cluster-of-differentiation antigen-9) in sperms of certain mammalian species has been attributed to its fusion with the egg and thereby dictating the fertility of species. In the present study, we investigated the association of CD9 with crossbred bull sperm quality and quantity trait was analyzed using a total of 96 Frieswal (HF × Sahiwal) crossbred. A single nucleotide polymorphism (g.358A > T) in intron 6 was significantly associated with sperm concentration (P < 0.05) and motility percentage (P < 0.01). mRNA was extracted from good (progressive motility > 50%) and motility impaired (progressive motility < 50%) bull semen. The mRNA expression and seminal plasma protein concentration of CD9 was significantly (P < 0.05) higher among good quality bull semen than motility impaired ones. Our results thus may indicate that, mutation in the intronic region may be responsible for the instability of RNA and the subsequent functional protein expression.
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Umesh
authors.initials: U
authors.lastname: Singh
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Rajib
authors.initials: R
authors.lastname: Deb
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Tyagi
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Mandal
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: G
authors.initials: G
authors.lastname: Sengar
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Rani
authors.initials: R
authors.lastname: Singh
authors.affiliation: Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
authors.forename: Rupali
authors.initials: R
authors.lastname: Singh
keywords: Bull; CD9; Frieswal; Semen