title: Examining Roles of Glycans in Chemokine-Mediated Dendritic-Endothelial Cell Interactions.
abstract: Interactions between glycosaminoglycans (GAGs) and chemokines play a critical role in multiple physiological and pathological processes, including tumor metastasis and immune-cell trafficking. During our studies examining the genetic importance of the GAG subtype known as heparan sulfate (HS) on lymphatic endothelial cells (LECs), we established a repertoire of methods to assess how HS affects chemokine-mediated cell-cell interactions. In this chapter, we describe methods for monitoring migration and adhesion interactions of dendritic cells (DCs), the most potent antigen-presenting cells, with LECs. We will also report a methodology to assess chemokine-receptor interactions while incorporating approaches to target HS in the system. This includes in situ methods to visualize and quantify direct interactions between chemokines and chemokine receptors on DC surfaces, and how targeting HS produced by LECs or even DCs affects these interactions. These methods enable the mechanistic and functional characterization of GAG-chemokine interactions in cell-based studies that model physiologic interactions ex vivo. They may also be used to obtain novel insights into GAG-mediated biological processes.
authors.affiliation: Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, School of Pharmacy, Huaihai Institute of Technology, Lianyungang, China; VA San Diego Healthcare System, Medical and Research Sections, La Jolla, California, USA; Department of Medicine, Division of Pulmonary and Critical Care, University of California San Diego, La Jolla, California, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Yin
authors.affiliation: VA San Diego Healthcare System, Medical and Research Sections, La Jolla, California, USA; Department of Medicine, Division of Pulmonary and Critical Care, University of California San Diego, La Jolla, California, USA.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Johns
authors.affiliation: VA San Diego Healthcare System, Medical and Research Sections, La Jolla, California, USA; Department of Medicine, Division of Pulmonary and Critical Care, University of California San Diego, La Jolla, California, USA.
authors.forename: Roland
authors.initials: R
authors.lastname: El Ghazal
authors.affiliation: Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA.
authors.forename: Catherina L
authors.initials: CL
authors.lastname: Salanga
authors.affiliation: Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA.
authors.forename: Tracy M
authors.initials: TM
authors.lastname: Handel
authors.affiliation: VA San Diego Healthcare System, Medical and Research Sections, La Jolla, California, USA; Department of Medicine, Division of Pulmonary and Critical Care, University of California San Diego, La Jolla, California, USA. Electronic address: mfuster@ucsd.edu.
authors.forename: Mark M
authors.initials: MM
authors.lastname: Fuster
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002450:Cell Communication; D002465:Cell Movement; D018925:Chemokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*; D011134:Polysaccharides / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism
chemical_list: D018925:Chemokines; D006497:Heparan Sulfate; D011134:Polysaccharides; D019707:Receptors, Chemokine
keywords: Adhesion; Chemokine; Dendritic; Endothelium; Heparin; Lymphatic; Traffic