title: A cluster of 3' coterminal transcripts from US12-US17 locus of human cytomegalovirus.
abstract: Among all the human cytomegalovirus (HCMV) gene families, US12 family is relatively undefined in their transcriptional profile and biological functions. In this study, the transcription pattern and characteristics of HCMV US12-US17 gene region were studied extensively. Twenty-three clones harboring US12 cDNA sequence were screened out from a late cDNA library of an HCMV clinical isolate, Han. Using a set of US12-US17 gene-specific probes, six transcripts from US12-US17 locus were detected by northern blot at late kinetics of the clinical isolate. One additional transcript was found in late RNA of HCMV strain AD169. No evidence showing these transcripts contain introns by reverse transcription PCR. 3' and 5' termini of these transcripts were confirmed by Rapid Amplification of cDNA Ends. A novel protein-coding region was predicted in the shorter US14 transcript with an alternative in-frame 5' translation initiation site compared to that of the previously predicted US14 ORF. Our findings demonstrate the existence of a cluster of 3' coterminal unspliced transcripts with distinct 5' transcriptional initiation sites originated from US12-US17 gene region in the late infection phase of an HCMV clinical strain.
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China.|Department of Pathogen Biology, College of Basic Medical Sciences, Liaoning Medical University, Jinzhou, Liaoning, 121000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China. mayp@sj-hospital.org.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Han
authors.affiliation: The Clinical Laboratory, Shenyang Women's and Children's Hospital, Shenyang, Liaoning, 110014, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Gao
authors.affiliation: The Pediatric Department, Shenyang Women's and Children's Hospital, Shenyang, Liaoning, 110014, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning, 121000, China. ruanq@sj-hospital.org.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D015870:Gene Expression; D015723:Gene Library; D005814:Genes, Viral; D056426:Genetic Loci; D006801:Humans; D007438:Introns; D008562:Membrane Glycoproteins / Q000235:genetics; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins
keywords: Cytomegalovirus; Open reading frames; Transcript; US12–US17