title: Engineering the shikimate pathway for biosynthesis of molecules with pharmaceutical activities in E. coli.
abstract: Engineering the shikimate pathway is a primary approach for biosynthesis of various aromatic compounds, many of which are involved in formation of important compounds with pharmaceutical values. The development of metabolic engineering allows for high-efficiency production of desired molecules derived from the shikimate pathway using engineered microbes as biosynthetic factories. This review summarizes successful and generally applicable strategies for engineering this important pathway in the context of the model bacterium E. coli for biosynthesis of molecules with pharmaceutical activities. Similar approaches can also be employed for shikimate pathway engineering in other microorganisms.
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China. Electronic address: jiangming9722@sjtu.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, NJ 08854, USA. Electronic address: Haoran.Zhang@Rutgers.edu.
authors.forename: Haoran
authors.initials: H
authors.lastname: Zhang
mesh_terms: D053898:Biosynthetic Pathways; D004926:Escherichia coli / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D064688:Metabolic Flux Analysis; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D012765:Shikimic Acid / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004364:Pharmaceutical Preparations; D012765:Shikimic Acid