title: Direct visualization of replication dynamics in early zebrafish embryos.
abstract: We analyzed DNA replication in early zebrafish embryos. The replicating DNA of whole embryos was labeled with the thymidine analog 5-ethynyl-2'-deoxyuridine (EdU), and spatial regulation of replication sites was visualized in single embryo-derived cells. The results unveiled uncharacterized replication dynamics during zebrafish early embryogenesis.
authors.affiliation: a Laboratory of Molecular & Cellular Biology, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuriya
authors.affiliation: a Laboratory of Molecular & Cellular Biology, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Higashiyama
authors.affiliation: b Laboratory for the Utilization of Aquatic Bioresources, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Avşar-Ban
authors.affiliation: a Laboratory of Molecular & Cellular Biology, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Nanami
authors.initials: N
authors.lastname: Okochi
authors.affiliation: a Laboratory of Molecular & Cellular Biology, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Kaede
authors.initials: K
authors.lastname: Hattori
authors.affiliation: a Laboratory of Molecular & Cellular Biology, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Ogata
authors.affiliation: c Department of Biochemistry and Proteomics , Graduate School of Medicine, Mie University , Tsu , Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Takebayashi
authors.affiliation: c Department of Biochemistry and Proteomics , Graduate School of Medicine, Mie University , Tsu , Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Ogata
authors.affiliation: b Laboratory for the Utilization of Aquatic Bioresources, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
authors.affiliation: a Laboratory of Molecular & Cellular Biology, Department of Life Sciences , Graduate School of Bioresources, Mie University , Tsu , Japan.
authors.forename: Katsuzumi
authors.initials: K
authors.lastname: Okumura
mesh_terms: D000818:Animals; D004261:DNA Replication; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism* / Q000648:ultrastructure; D047108:Embryonic Development / Q000235:genetics*; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
chemical_list: C031086:5-ethynyl-2'-deoxyuridine; D003857:Deoxyuridine
keywords: DNA replication; replication foci; zebrafish embryo