title: Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam.
abstract: Little information is available on the epidemiology of Giardia duodenalis in beef cattle from Vietnam. This study was performed to determine the prevalence and genotypes/assemblages of G. duodenalis in native beef calves younger than 6 months in the region. A total of 412 calf fecal samples, randomly selected from 99 small-scale farms located in DacLac and KhanhHoa provinces, central Vietnam, were screened for the presence of G. duodenalis cysts using the zinc-sulfate flotation method followed by iodine staining. The overall prevalence on the sample and herd levels were 13.8% (57/412) and 42.4% (42/99), respectively. Molecular analysis in the β-giardin and triosephosphate isomerase genes demonstrated the presence of only G. duodenalis assemblage E in the animals. Since assemblage E has been rarely reported in humans, the zoonotic risk in beef calves in the region appears to be minimal.
authors.affiliation: Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dongde street, Nhatrang, Vietnam.
authors.forename: Sam Thi
authors.initials: ST
authors.lastname: Nguyen
authors.affiliation: Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-Onsen, Osaki, Miyagi, 989-6711, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fukuda
authors.affiliation: Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dongde street, Nhatrang, Vietnam.
authors.forename: Duc Tan
authors.initials: DT
authors.lastname: Nguyen
authors.affiliation: Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-Onsen, Osaki, Miyagi, 989-6711, Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Tada
authors.affiliation: Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-Onsen, Osaki, Miyagi, 989-6711, Japan. nakai@bios.tohoku.ac.jp.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005838:Genotype; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000662:veterinary*; D015995:Prevalence; D000069466:Red Meat; D014744:Vietnam / Q000453:epidemiology
keywords: Calves; Genotyping; Giardia duodenalis; Prevalence; Vietnam