title: Selective Killing Effects of Cold Atmospheric Pressure Plasma with NO Induced Dysfunction of Epidermal Growth Factor Receptor in Oral Squamous Cell Carcinoma.
abstract: The aim of this study is to investigate the effects of cold atmospheric pressure plasma (CAP)-induced radicals on the epidermal growth factor receptor (EGFR), which is overexpressed by oral squamous cell carcinoma, to determine the underlying mechanism of selective killing. CAP-induced highly reactive radicals were observed in both plasma plume and cell culture media. The selective killing effect was observed in oral squamous cell carcinoma compared with normal human gingival fibroblast. Degradation and dysfunction of EGFRs were observed only in the EGFR-overexpressing oral squamous cell carcinoma and not in the normal cell. Nitric oxide scavenger pretreatment in cell culture media before CAP treatment rescued above degradation and dysfunction of the EGFR as well as the killing effect in oral squamous cell carcinoma. CAP may be a promising cancer treatment method by inducing EGFR dysfunction in EGFR-overexpressing oral squamous cell carcinoma via nitric oxide radicals.
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, Brain Korea 21 PLUS project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.|Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Republic of Korea.
authors.forename: Jung-Hwan
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, Brain Korea 21 PLUS project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Om
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, 20 Kwangwoon-gil, Nowon-gu, Seoul, 139-701, Republic of Korea.
authors.forename: Yong-Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, Brain Korea 21 PLUS project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
authors.forename: Kwang-Mahn
authors.initials: KM
authors.lastname: Kim
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, 20 Kwangwoon-gil, Nowon-gu, Seoul, 139-701, Republic of Korea.
authors.forename: Eun-Ha
authors.initials: EH
authors.lastname: Choi
authors.affiliation: Department and Research Institute of Dental Biomaterials and Bioengineering, Brain Korea 21 PLUS project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
authors.forename: Kyoung-Nam
authors.initials: KN
authors.lastname: Kim
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D066246:ErbB Receptors / Q000037:antagonists & inhibitors*; D005347:Fibroblasts / Q000187:drug effects; D005609:Free Radicals; D005881:Gingiva / Q000166:cytology; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D018384:Oxidative Stress; D058626:Plasma Gases / Q000494:pharmacology* / Q000627:therapeutic use; D059748:Proteolysis; D026361:Reactive Nitrogen Species / Q000494:pharmacology*; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D005609:Free Radicals; D009363:Neoplasm Proteins; D058626:Plasma Gases; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; C512478:EGFR protein, human; D066246:ErbB Receptors; D000111:Acetylcysteine