title: In vivo and in vitro infection dynamics of honey bee viruses.
abstract: The honey bee (Apis mellifera) is commonly infected by multiple viruses. We developed an experimental system for the study of such mixed viral infections in newly emerged honey bees and in the cell line AmE-711, derived from honey bee embryos. When inoculating a mixture of iflavirids [sacbrood bee virus (SBV), deformed wing virus (DWV)] and dicistrovirids [Israeli acute paralysis virus (IAPV), black queen cell virus (BQCV)] in both live bee and cell culture assays, IAPV replicated to higher levels than other viruses despite the fact that SBV was the major component of the inoculum mixture. When a different virus mix composed mainly of the dicistrovirid Kashmir bee virus (KBV) was tested in cell culture, the outcome was a rapid increase in KBV but not IAPV. We also sequenced the complete genome of an isolate of DWV that covertly infects the AmE-711 cell line, and found that this virus does not prevent IAPV and KBV from accumulating to high levels and causing cytopathic effects. These results indicate that different mechanisms of virus-host interaction affect virus dynamics, including complex virus-virus interactions, superinfections, specific virus saturation limits in cells and virus specialization for different cell types.
authors.affiliation: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.|Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Jimena
authors.initials: J
authors.lastname: Carrillo-Tripp
authors.affiliation: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Adam G
authors.initials: AG
authors.lastname: Dolezal
authors.affiliation: Department of Entomology, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Goblirsch
authors.affiliation: Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, USA.
authors.forename: W Allen
authors.initials: WA
authors.lastname: Miller
authors.affiliation: Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, IA 50011, USA.|Department of Entomology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Amy L
authors.initials: AL
authors.lastname: Toth
authors.affiliation: Department of Entomology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Bryony C
authors.initials: BC
authors.lastname: Bonning
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D002460:Cell Line; D002478:Cells, Cultured; D007304:Insect Viruses / Q000502:physiology*