title: Ypq3p-dependent histidine uptake by the vacuolar membrane vesicles of Saccharomyces cerevisiae.
abstract: The vacuolar membrane proteins Ypq1p, Ypq2p, and Ypq3p of Saccharomyces cerevisiae are known as the members of the PQ-loop protein family. We found that the ATP-dependent uptake activities of arginine and histidine by the vacuolar membrane vesicles were decreased by ypq2Δ and ypq3Δ mutations, respectively. YPQ1 and AVT1, which are involved in the vacuolar uptake of lysine/arginine and histidine, respectively, were deleted in addition to ypq2Δ and ypq3Δ. The vacuolar membrane vesicles isolated from the resulting quadruple deletion mutant ypq1Δypq2Δypq3Δavt1Δ completely lost the uptake activity of basic amino acids, and that of histidine, but not lysine and arginine, was evidently enhanced by overexpressing YPQ3 in the mutant. These results suggest that Ypq3p is specifically involved in the vacuolar uptake of histidine in S. cerevisiae. The cellular level of Ypq3p-HA(3) was enhanced by depletion of histidine from culture medium, suggesting that it is regulated by the substrate.
authors.affiliation: a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Kunio
authors.initials: K
authors.lastname: Manabe
authors.affiliation: a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.|b Advanced Research Support Center (ADRES), Ehime University , Matsuyama , Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano-Kawada
authors.affiliation: a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ikeda
authors.affiliation: a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.affiliation: a Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture , Ehime University , Matsuyama , Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D026905:Amino Acid Transport Systems / Q000172:deficiency / Q000235:genetics*; D017920:Antiporters / Q000172:deficiency / Q000235:genetics*; D001120:Arginine / Q000378:metabolism; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D022162:Cytoplasmic Vesicles / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D006639:Histidine / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics*; D020033:Protein Isoforms / Q000172:deficiency / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D014617:Vacuoles / Q000378:metabolism
chemical_list: D026905:Amino Acid Transport Systems; D017920:Antiporters; C000607320:Avt1 protein, S cerevisiae; D008565:Membrane Proteins; D020033:Protein Isoforms; D029701:Saccharomyces cerevisiae Proteins; C000598342:Ypq1 protein, S cerevisiae; D006639:Histidine; D000255:Adenosine Triphosphate; D001120:Arginine; D008239:Lysine
keywords: PQ-loop; histidine; vacuolar transporter