title: Resistin Induces Hypertension and Insulin Resistance in Mice via a TLR4-Dependent Pathway.
abstract: Resistin, an adipokine involved in insulin resistance (IR) and diabetes, has recently been reported to play a role in cardiovascular events. However, its effect on blood pressure (BP) and the underlying mechanisms remain unclear. In the present study, we showed that resistin induces hypertension and IR in wild type (WT) mice, but not in tlr4(-/-) mice. Resistin upregulated angiotensinogen (Agt) expression in WT mice, whereas it had no effect on tlr4(-/-) mice, or in mice treated with the angiotensin-converting enzyme inhibitor perindopril. Real-time PCR and chromatin immunoprecipitation further confirmed that resistin activates the renin-angiotensin system (RAS) via the TLR4/P65/Agt pathway. This finding suggested an essential role of resistin in linking IR and hypertension, which may offer a novel target in clinic on the study of the association between diabetes and hypertension.
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Linfang
authors.initials: L
authors.lastname: Lu
authors.affiliation: Clinical Laboratory, the Third Hospital of Wuhan, Wuhan, China.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Chaoqiang
authors.initials: C
authors.lastname: An
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yu
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Le
authors.initials: L
authors.lastname: Shu
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Ao
authors.initials: A
authors.lastname: Chen
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Niu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Zaiqing
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000808:Angiotensinogen / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D045744:Cell Line, Tumor; D056945:Hep G2 Cells; D006801:Humans; D006973:Hypertension / Q000473:pathology*; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020913:Perindopril / Q000494:pharmacology*; D012084:Renin-Angiotensin System / Q000187:drug effects / Q000502:physiology*; D052243:Resistin / Q000494:pharmacology*; D051197:Toll-Like Receptor 4 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: C534932:Agt protein, mouse; D000806:Angiotensin-Converting Enzyme Inhibitors; C498144:Rela protein, mouse; D052243:Resistin; C499623:Retn protein, mouse; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D051996:Transcription Factor RelA; D000808:Angiotensinogen; D020913:Perindopril