title: Sphingomonas metalli sp. nov., isolated from an abandoned lead-zinc mine.
abstract: A Gram-stain-negative and non-motile bacterial strain designated 9O-5T was isolated from an abandoned lead-zinc mine in Meizhou, Guangdong Province, southern China. The isolate was orange-pigmented, aerobic, and oxidase- and catalase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 9O-5T belongs to the genus Sphingomonas and was closely related to Sphingomonas abaci DSM 15867T (97.6 % similarity), Sphingomonas phyllosphaerae FA2T (96.9 %) and Shingomonas guangdongensis 9NM-8T (96.8 %). Mean DNA-DNA relatedness between strain 9O-5T and S. abaci DSM 15867T was only 47.1 ± 4.9 %. The major fatty acids were C18 : 1ω7c, C14 : 0 2-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). It contained Q-10 as the predominant respiratory quinone and sym-homospermidine as the major polyamine. The polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unidentified phospholipids and six unidentified lipids. The genomic DNA G+C content of strain 9O-5T was 69.1 ± 0.1 mol%. Based on the data from this polyphasic taxonomic study, strain 9O-5T should be considered as representing a novel species of the genus Sphingomonas, for which the name Sphingomonas metalli sp. nov. is proposed. The type strain is 9O-5T ( = CGMCC 1.15330T = KCTC 42759T).
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology,Guangzhou 510070, PRChina.|College of Agriculture, South China Agricultural University,Guangzhou 510642, PRChina.
authors.forename: Guang-Da
authors.initials: GD
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology,Guangzhou 510070, PRChina.
authors.forename: Song-Zhen
authors.initials: SZ
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology,Guangzhou 510070, PRChina.
authors.forename: Xiong
authors.initials: X
authors.lastname: Xiong
authors.affiliation: College of Agriculture, South China Agricultural University,Guangzhou 510642, PRChina.
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Li
authors.affiliation: State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology,Guangzhou 510070, PRChina.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Zhu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007854:Lead; D008906:Mining; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013095:Spermidine / Q000031:analogs & derivatives / Q000737:chemistry; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry; D015032:Zinc
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D014451:Ubiquinone; D007854:Lead; C024989:coenzyme Q10; D015032:Zinc; D013095:Spermidine