title: One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle.
abstract: Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.
authors.affiliation: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.|Institute for Laboratory Animal Sciences, Medical School Hannover (MHH), Germany.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Garrels
authors.affiliation: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.
authors.forename: Thirumala R
authors.initials: TR
authors.lastname: Talluri
authors.affiliation: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.
authors.forename: Ronja
authors.initials: R
authors.lastname: Apfelbaum
authors.affiliation: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.|Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors.forename: Yanet P
authors.initials: YP
authors.lastname: Carratalá
authors.affiliation: Departamento de Biología Molecular, FCEFQyN, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Bosch
authors.affiliation: Paul-Ehrlich-Institute, Langen, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Pötzsch
authors.affiliation: Paul-Ehrlich-Institute, Langen, Germany.
authors.forename: Esther
authors.initials: E
authors.lastname: Grueso
authors.affiliation: Paul-Ehrlich-Institute, Langen, Germany.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Ivics
authors.affiliation: Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.
authors.forename: Wilfried A
authors.initials: WA
authors.lastname: Kues
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001755:Blastocyst / Q000502:physiology; D002417:Cattle; D004251:DNA Transposable Elements; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D016678:Genome; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008845:Microinjections; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D019076:Transgenes; D019895:Transposases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D019895:Transposases