title: Prospective Study of Human Polyomaviruses and Risk of Cutaneous Squamous Cell Carcinoma in the United States.
abstract: Merkel cell polyomavirus (PyV) is causally related to Merkel cell carcinoma, a rare skin malignancy. Little is known about the serostability of other PyVs over time or associations with cutaneous squamous cell carcinoma (SCC).
  
  
  
  As part of a U.S. nested case-control study, antibody response against the PyV VP1 capsid proteins of BK and John Cunningham virus (JC) was measured using multiplex serology on 113 SCC cases and 229 gender, age, and study center-matched controls who had a prior keratinocyte cancer. Repeated serum samples from controls and both pre and postdiagnosis samples from a subset of SCC cases were also tested. Odds ratios (OR) for SCC associated with seropositivity to each PyV type were estimated using conditional logistic regression.
  
  
  
  Among controls, BK and JC seroreactivity was stable over time, with intraclass correlation coefficients of 0.86 for BK and 0.94 for JC. Among cases, there was little evidence of seroconversion following SCC diagnosis. JC seropositivity prior to diagnosis was associated with an elevated risk of SCC (OR = 2.54; 95% CI, 1.23-5.25), and SCC risk increased with increasing quartiles of JC (Ptrend = 0.004) and BK (Ptrend = 0.02) seroreactivity.
  
  
  
  PyV antibody levels were stable over time and following an SCC diagnosis. A history of PyV infection may be involved in the occurrence of SCC in a population at high risk for this malignancy.
  
  
  
  A single measure of PyV seroreactivity appears a reliable indicator of long-term antibody status, and PyV exposure may be a risk factor for subsequent SCC. Cancer Epidemiol Biomarkers Prev; 25(5); 736-44. ©2016 AACR.
authors.affiliation: Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire.
authors.forename: Anala
authors.initials: A
authors.lastname: Gossai
authors.affiliation: German Cancer Research Center (Deutsches Krebsforschungszentrum), Heidelberg, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Waterboer
authors.affiliation: Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota.
authors.forename: Heather H
authors.initials: HH
authors.lastname: Nelson
authors.affiliation: Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire. Department of Community and Family Medicine, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Doherty
authors.affiliation: German Cancer Research Center (Deutsches Krebsforschungszentrum), Heidelberg, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Michel
authors.affiliation: German Cancer Research Center (Deutsches Krebsforschungszentrum), Heidelberg, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Willhauck-Fleckenstein
authors.affiliation: Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire. Langone Medical Center, New York University, New York, New York.
authors.forename: Shohreh F
authors.initials: SF
authors.lastname: Farzan
authors.affiliation: Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire. Department of Community and Family Medicine, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire. Department of Pharmacology and Toxicology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire.
authors.forename: Brock C
authors.initials: BC
authors.lastname: Christensen
authors.affiliation: Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire. Department of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire.
authors.forename: Anne G
authors.initials: AG
authors.lastname: Hoen
authors.affiliation: Geisel School of Medicine at Dartmouth, Hanover, New Hampshire.
authors.forename: Ann E
authors.initials: AE
authors.lastname: Perry
authors.affiliation: German Cancer Research Center (Deutsches Krebsforschungszentrum), Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Pawlita
authors.affiliation: Department of Epidemiology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire. Department of Community and Family Medicine, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire. Margaret.R.Karagas@dartmouth.edu.
authors.forename: Margaret R
authors.initials: MR
authors.lastname: Karagas
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000209:etiology* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D059965:Merkel cell polyomavirus / Q000276:immunology*; D008875:Middle Aged; D027601:Polyomavirus Infections / Q000150:complications* / Q000821:virology; D011446:Prospective Studies; D012878:Skin Neoplasms / Q000209:etiology* / Q000473:pathology; D014481:United States