title: Nutritional and exercise interventions variably affect estrogen receptor expression in the adipose tissue of male rats.
abstract: Energy-dense food consumption and lack of physical activity are implicated in the development of the current obesity epidemic. The role of estrogen in adiposity and fuel partitioning is mediated mainly though the estrogen receptor α (ERα) isoform. We hypothesized that nutritional adaptation and exercise training, either individually or combined, could impact ERα expression in adipose tissue relative to glucose tolerance. Seventy-two Wistar rats were submitted to a high-fat, high-sucrose (HF-HS) diet for 16weeks. The first phase of our study was to investigate the effect of an HF-HS diet on whole-body glucose tolerance, as well as on body composition and ERα expression in different adipose tissues. Second, we investigated the effect of switching to a well-balanced diet, with or without exercise training for 8 weeks, on those same parameters. After the first part of this study, HF-HS-fed rats were fatter (8%) than control rats. Despite a decrease in glucose tolerance, ERα expression in adipose tissues was not significantly altered by an HF-HS diet. The return to a well-balanced diet significantly increased ERα expression in perirenal and epididymal adipose tissue, but there was no effect of diet or exercise training on whole-body glucose tolerance. The present findings suggest that diet is a powerful modulator of ERα expression in adipose tissue, as nutritional modulation after an HF-HS diet strongly affects ERα expression, particularly in perirenal and epididymal adipose tissue. However, ERα expression in adipose tissue does not appear to be associated with whole-body glucose tolerance.
authors.affiliation: Clermont Université, Université Blaise Pascal, EA3533, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques, CRNH Auvergne, F-63000 Clermont-Ferrand, France. Electronic address: Lore.METZ@univ-bpclermont.fr.
authors.forename: Lore
authors.initials: L
authors.lastname: Metz
authors.affiliation: Clermont Université, Université Blaise Pascal, EA3533, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques, CRNH Auvergne, F-63000 Clermont-Ferrand, France.
authors.forename: Maude
authors.initials: M
authors.lastname: Gerbaix
authors.affiliation: INRA, UMR1019, UNH, CRNH Auvergne, F-63000 Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Masgrau
authors.affiliation: INRA, UMR1019, UNH, CRNH Auvergne, F-63000 Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Guillet
authors.affiliation: INRA, UMR1019, UNH, CRNH Auvergne, F-63000 Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 Clermont-Ferrand, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Walrand
authors.affiliation: Clermont Université, Université Blaise Pascal, EA3533, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques, CRNH Auvergne, F-63000 Clermont-Ferrand, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Boisseau
authors.affiliation: INRA, UMR1019, UNH, CRNH Auvergne, F-63000 Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 Clermont-Ferrand, France; Clinical Nutrition Department, CHU Clermont-Ferrand, F-63000 Clermont-Ferrand, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Boirie
authors.affiliation: Clermont Université, Université Blaise Pascal, EA3533, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques, CRNH Auvergne, F-63000 Clermont-Ferrand, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Courteix
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage; D019422:Dietary Sucrose / Q000008:administration & dosage; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism; D020738:Leptin / Q000097:blood; D008297:Male; D009570:Nitriles / Q000097:blood; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D019422:Dietary Sucrose; D047628:Estrogen Receptor alpha; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D020738:Leptin; D009570:Nitriles; C036546:adiponitrile
keywords: Diet; Estrogen receptor; Exercise; Obesity; Rat