title: Expression of Mineralization Markers during Pulp Response to Biodentine and Mineral Trioxide Aggregate.
abstract: BACKGROUND
  The purpose of this study was to compare the cell viability of dental pulp cells treated with Biodentine (Septodont, Saint-Maur, France) and mineral trioxide aggregate (MTA) and the in vitro and in vivo expression of mineralization markers induced by the 2 materials.
  
  
  METHODS
  Human dental pulp cells isolated from 6 permanent teeth were stimulated with Biodentine and MTA extracts. Cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, and quantitative reverse-transcriptase polymerase chain reaction was used to determine the expression of mineralization markers. Specimens of teeth from dogs treated with Biodentine and MTA after pulpotomy were used to determine the presence of osteopontin and alkaline phosphatase by immunohistochemistry and runt-related transcription factor 2 by immunofluorescence.
  
  
  RESULTS
  No significant differences in cell viability were found between MTA and Biodentine extracts and controls after 24 and 48 hours (P > .05). After 48 hours, osteopontin (SPP1), alkaline phosphatase (ALP), and runt-related transcription factor 2 (RUNX2) expression was higher in MTA and Biodentine than in controls (P < .05). Osteopontin staining was more intense and spread over a greater number of areas in Biodentine than in MTA samples (P < .0001). Alkaline phosphatase staining of a mineralized tissue bridge was significantly different between materials (P < .0001), but no difference in alkaline phosphatase staining of pulp tissue was found between MTA and Biodentine (P = .2). Also, no significant difference in the number of cells labeled for runt-related transcription factor 2 by immunofluorescence was observed between materials (P > .05).
  
  
  CONCLUSIONS
  Biodentine stimulated similar markers as MTA, but staining was more intense and spread over a larger area of the pulp tissue.
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Mariana O
authors.initials: MO
authors.lastname: Daltoé
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil; Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Francisco Wanderley G
authors.initials: FW
authors.lastname: Paula-Silva
authors.affiliation: Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Lúcia H
authors.initials: LH
authors.lastname: Faccioli
authors.affiliation: University of Barcelona, Barcelona, Spain.
authors.forename: Patrícia M
authors.initials: PM
authors.lastname: Gatón-Hernández
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Andiara
authors.initials: A
authors.lastname: De Rossi
authors.affiliation: University of São Paulo, School of Dentistry of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil. Electronic address: lea@forp.usp.br.
authors.forename: Léa Assed
authors.initials: LA
authors.lastname: Bezerra Silva
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D017610:Calcium Compounds / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D003782:Dental Pulp / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D004338:Drug Combinations; D005260:Female; D006801:Humans; D008297:Male; D053495:Osteopontin / Q000096:biosynthesis*; D010087:Oxides / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D017640:Silicates / Q000494:pharmacology*; D019227:Tooth Apex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D017607:Aluminum Compounds; D015415:Biomarkers; D017610:Calcium Compounds; D050659:Core Binding Factor Alpha 1 Subunit; D004338:Drug Combinations; D010087:Oxides; C493709:RUNX2 protein, human; D017640:Silicates; C086631:mineral trioxide aggregate; D053495:Osteopontin; C506393:tricalcium silicate; D000469:Alkaline Phosphatase
keywords: Biodentine; dental pulp; mineral trioxide aggregate; mineralization; pulpotomy