title: Peroxisome proliferator-activated receptor γ activation favours selective subcutaneous lipid deposition by coordinately regulating lipoprotein lipase modulators, fatty acid transporters and lipogenic enzymes.
abstract: Peroxisome proliferator-activated receptor (PPAR) γ activation is associated with preferential lipoprotein lipase (LPL)-mediated fatty acid storage in peripheral subcutaneous fat depots. How PPARγ agonism acts upon the multi-level modulation of depot-specific lipid storage remains incompletely understood.
  
  
  
  We evaluated herein triglyceride-derived lipid incorporation into adipose tissue depots, LPL mass and activity, mRNA levels and content of proteins involved in the modulation of LPL activity and fatty acid transport, and the expression/activity of enzymes defining adipose tissue lipogenic potential in rats treated with the PPARγ ligand rosiglitazone (30 mg kg(-1)  day(-1) , 23 days) after either a 10-h fasting period or a 17-h fast followed by 6 h of ad libitum refeeding.
  
  
  
  Rosiglitazone stimulated lipid accretion in subcutaneous fat (SF) ~twofold and significantly reduced that of visceral fat (VF) to nearly half. PPARγ activation selectively increased LPL mass, activity and the expression of its chaperone LMF1 in SF. In VF, rosiglitazone had no effect on LPL activity and downregulated the mRNA levels of the transendothelial transporter GPIHBP1. Overexpression of lipid uptake and fatty acid transport proteins (FAT/CD36, FATP1 and FABP4) and stimulation of lipogenic enzyme activities (GPAT, AGPAT and DGAT) upon rosiglitazone treatment were of higher magnitude in SF.
  
  
  
  Together these findings demonstrate that the depot-specific transcriptional control of LPL induced by PPARγ activation extends to its key interacting proteins and post-translational modulators to favour subcutaneous lipid storage.
authors.affiliation: Department of Medicine, Faculty of Medicine, Quebec Heart and Lung Institute, Laval University, Quebec, QC, Canada.
authors.forename: P G
authors.initials: PG
authors.lastname: Blanchard
authors.affiliation: Department of Medicine, Faculty of Medicine, Quebec Heart and Lung Institute, Laval University, Quebec, QC, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Turcotte
authors.affiliation: Department of Medicine, Faculty of Medicine, Quebec Heart and Lung Institute, Laval University, Quebec, QC, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Côté
authors.affiliation: Department of Medicine, Faculty of Medicine, Quebec Heart and Lung Institute, Laval University, Quebec, QC, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Gélinas
authors.affiliation: Department of Medical Biosciences/Physiological Chemistry, Umeå University, Umeå, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Nilsson
authors.affiliation: Department of Medical Biosciences/Physiological Chemistry, Umeå University, Umeå, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Olivecrona
authors.affiliation: Department of Medicine, Faculty of Medicine, Quebec Heart and Lung Institute, Laval University, Quebec, QC, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Deshaies
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: W T
authors.initials: WT
authors.lastname: Festuccia
mesh_terms: D000818:Animals; D050612:Fatty Acid Transport Proteins / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D050155:Lipogenesis / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D047495:PPAR gamma / Q000819:agonists*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018110:Receptors, Lipoprotein / Q000096:biosynthesis / Q000235:genetics; D000077154:Rosiglitazone; D050151:Subcutaneous Fat / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D045162:Thiazolidinediones / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
chemical_list: D050612:Fatty Acid Transport Proteins; C579044:GPIHBP1 protein, rat; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D018110:Receptors, Lipoprotein; D045162:Thiazolidinediones; D014280:Triglycerides; D000077154:Rosiglitazone; D008071:Lipoprotein Lipase
keywords: adipose tissue; fat redistribution; fatty acid transport; lipoprotein lipase; peroxisome proliferator-activated receptor; rosiglitazone