title: Glycerolized Reticular Dermis as a New Human Acellular Dermal Matrix: An Exploratory Study.
abstract: Human Acellular Dermal Matrices (HADM) are employed in various reconstructive surgery procedures as scaffolds for autologous tissue regeneration. The aim of this project was to develop a new type of HADM for clinical use, composed of glycerolized reticular dermis decellularized through incubation and tilting in Dulbecco's Modified Eagle's Medium (DMEM). This manufacturing method was compared with a decellularization procedure already described in the literature, based on the use of sodium hydroxide (NaOH), on samples from 28 donors. Cell viability was assessed using an MTT assay and microbiological monitoring was performed on all samples processed after each step. Two surgeons evaluated the biomechanical characteristics of grafts of increasing thickness. The effects of the different decellularization protocols were assessed by means of histological examination and immunohistochemistry, and residual DNA after decellularization was quantified using a real-time TaqMan MGB probe. Finally, we compared the results of DMEM based decellularization protocol on reticular dermis derived samples with the results of the same protocol applied on papillary dermis derived grafts. Our experimental results indicated that the use of glycerolized reticular dermis after 5 weeks of treatment with DMEM results in an HADM with good handling and biocompatibility properties.
authors.affiliation: Division of Breast Surgery, Department of General and Specialized Surgery, Città della Salute e della Scienza, Turin, Italy.
authors.forename: Pietro Maria
authors.initials: PM
authors.lastname: Ferrando
authors.affiliation: Division of Pathology, Department of Medical Sciences, University of Turin, Turin, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Balmativola
authors.affiliation: Skin Bank, Department of General and Specialized Surgery, Città della Salute e della Scienza, Turin, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Cambieri
authors.affiliation: Division of Pathology, Department of Medical Sciences, University of Turin, Turin, Italy.
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Scalzo
authors.affiliation: Cytoimmunodiagnostic Laboratory, Department of Public Health and Pediatrics, University of Turin, Turin, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Bergallo
authors.affiliation: Division of Pathology, Department of Medical Sciences, University of Turin, Turin, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Annaratone
authors.affiliation: Skin Bank, Department of General and Specialized Surgery, Città della Salute e della Scienza, Turin, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Casarin
authors.affiliation: Skin Bank, Department of General and Specialized Surgery, Città della Salute e della Scienza, Turin, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Fumagalli
authors.affiliation: Skin Bank, Department of General and Specialized Surgery, Città della Salute e della Scienza, Turin, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Stella
authors.affiliation: Division of Pathology, Department of Medical Sciences, University of Turin, Turin, Italy.|Fondazione del Piemonte per l'Oncologia (FPO) - Candiolo Cancer Institute (IRCCs), Candiolo, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Sapino
authors.affiliation: Skin Bank, Department of General and Specialized Surgery, Città della Salute e della Scienza, Turin, Italy.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Castagnoli
mesh_terms: D062088:Acellular Dermis / Q000378:metabolism* / Q000382:microbiology; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006801:Humans; D016038:Skin Transplantation; D013997:Time Factors; D054457:Tissue Scaffolds
chemical_list: D004247:DNA; D005990:Glycerol