title: The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells.
abstract: Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors involved in colonization are adhesins such as the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1) as a novel interaction partner for SdrD. Genetic deletion of sdrD in S. aureus NCTC8325-4 through allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells in vitro. Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells resulted in increased adherence of S. aureus NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4ΔsdrD, to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface. These data demonstrate that Dsg1 is a host ligand for SdrD.
authors.affiliation: Research group of Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT-The Artic University of Norway, Norway.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Askarian
authors.affiliation: Research group of Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT-The Artic University of Norway, Norway.
authors.forename: Clement
authors.initials: C
authors.lastname: Ajayi
authors.affiliation: Research group of Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT-The Artic University of Norway, Norway.
authors.forename: Anne-Merethe
authors.initials: AM
authors.lastname: Hanssen
authors.affiliation: Medical Microbiology, University Medical Center Utrecht, Utrecht 3584CX, The Netherlands.
authors.forename: Nina M
authors.initials: NM
authors.lastname: van Sorge
authors.affiliation: Research group of Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT-The Artic University of Norway, Norway.
authors.forename: Ingvild
authors.initials: I
authors.lastname: Pettersen
authors.affiliation: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Science, Ås, Norway.
authors.forename: Dzung B
authors.initials: DB
authors.lastname: Diep
authors.affiliation: Research group of Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT-The Artic University of Norway, Norway.
authors.forename: Johanna U E
authors.initials: JU
authors.lastname: Sollid
authors.affiliation: Research group of Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT-The Artic University of Norway, Norway.
authors.forename: Mona
authors.initials: M
authors.lastname: Johannessen
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D051183:Desmoglein 1 / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D006801:Humans; D013294:Lactococcus lactis / Q000235:genetics / Q000502:physiology; D008024:Ligands; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; C495297:DSG1 protein, human; D051183:Desmoglein 1; D008024:Ligands; D011994:Recombinant Proteins; C115915:SdrD protein, Staphylococcus aureus