title: Genome-wide analysis of aberrantly expressed microRNAs in bronchoalveolar lavage fluid from patients with silicosis.
abstract: Background To identify differentially expressed miRNAs profiles in bronchoalveolar lavage fluid (BALF) from patients with silicosis and consider the potential contribution of miRNAs to silicosis.Methods miRNAs expression profiling were performed in the cell fraction of BALF samples obtained from 9 subjects (3 silicosis observation subjects, 3 stage I and stage II silicosis patients, respectively). The differential expression of two selected miRNAs hsa-miR-181c-5p and hsa-miR-29a-3p were confirmed by RT-qPCR. Furthermore, miRNAs Gene Ontology Enrichment categories and target mRNAs were determined based on miRWalk.Results We found 110 dysregulated miRNAs in silicosis samples, most of which showed a down-regulation trend. Microarray results were confirmed by RT-qPCR. With the observation group samples set as standards, stage I samples showed 123 differentially expressed miRNAs, and stage II 46. 23 miRNAs were dysregulated in both stages. Finally, functional enrichment analysis indicated that these miRNAs played an important role in various biological processes, including ECM-receptor interaction and endocytosis.Conclusions This is the first time to acquire the BALF-derived microRNAs expression profiling targeting to human silicosis. These results contribute to unravelling miRNAs involved in the pathogenesis of silicosis, and provide new tools of potential use of as biomarkers for diagnosis and/or therapeutic purposes.
authors.affiliation: Department of Occupational and Environmental Medicine, West China School of Public Health, Sichuan University, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Faxuan
authors.initials: F
authors.lastname: Wang
authors.forename: Dingzi
authors.initials: D
authors.lastname: Zhou
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ren
authors.forename: Dinglun
authors.initials: D
authors.lastname: Zhou
authors.forename: Xiaosi
authors.initials: X
authors.lastname: Gao
authors.forename: Yajia
authors.initials: Y
authors.lastname: Lan
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Xie
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D004705:Endocytosis / Q000235:genetics; D005109:Extracellular Matrix / Q000378:metabolism; D020869:Gene Expression Profiling; D015894:Genome, Human; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012829:Silicosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; C532045:MIRN29a microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D011956:Receptors, Cell Surface