title: Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium - Short communication.
abstract: The aim of the present pilot study was to apply a flow cytometric assay, the so-called OxyDNA test, to determine the level of oxidative DNA damage in fish spermatozoa exposed to different concentrations (0.01-10,000 mg/L) of cadmium. Milt was collected from three randomly selected Prussian carp (Carassius auratus gibelio) males. Oxidative DNA damage was assessed with the OxyDNA kit and using flow cytometry. The ratio of OxyDNA-positive events increased significantly at higher cadmium concentrations. The results indicate that direct contact of fish spermatozoa with cadmium-polluted water initiates genotoxic damage.
authors.affiliation: Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia , Deák F. u. 16, H-8360 Keszthely , Hungary.
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Nagy
authors.affiliation: Department of Limnology, Institute of Environmental Sciences, University of Pannonia , Veszprém , Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kakasi
authors.affiliation: Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia , Deák F. u. 16, H-8360 Keszthely , Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Bercsényi
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D002469:Cell Separation / Q000379:methods / Q000662:veterinary; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D008297:Male; D018384:Oxidative Stress; D010865:Pilot Projects; D012661:Semen / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D002104:Cadmium
keywords: Cadmium; DNA damage; OxyDNA; fish sperm; flow cytometry