title: The Brucella melitensis M5-90 phosphoglucomutase (PGM) mutant is attenuated and confers protection against wild-type challenge in BALB/c mice.
abstract: Brucellae are Gram-negative intracellular bacterial pathogens that infect humans and animals, bringing great economic burdens to developing countries. Live attenuated Brucella vaccines (strain M5-90 or others) are the most efficient means for prevention and control of animal brucellosis. However, these vaccines have several drawbacks, including residual virulence in animals, and difficulties in differentiating natural infection from vaccine immunization, which limit their application. A vaccine that can differentiate infection from immunization will have extensive applications. A Brucella melitensis (B. melitensis) strain M5-90 pgm mutant (M5-90Δpgm) was constructed to overcome these drawbacks. M5-90Δpgm showed significantly reduced survival in embryonic trophoblast cells and in mice, and induced high protective immunity in BALB/c mice. Moreover, M5-90Δpgm elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon (IFN-γ) and interleukin-2 (IL-2). In addition, M5-90Δpgm induced the secretion of IFN-γ in immunized sheep. Serum samples from sheep inoculated with M5-90Δpgm were negative by the Rose Bengal Plate Test (RBPT) and Standard Tube Agglutination Test (STAT). Furthermore, the PGM antigen allowed serological differentiation between infected and vaccinated animals. These results suggest that M5-90Δpgm is an ideal live attenuated vaccine candidate against B. melitensis 16 M and deserves further evaluation for vaccine development.
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Tiansen
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Shangqiu Normal University, Shangqiu, 476000, Henan Province, People's Republic of China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Hanping
authors.initials: H
authors.lastname: Feng
authors.affiliation: College of Medicine, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Yuanzhi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China.
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Shihezi University, Shihezi, 832000, Xinjiang Province, People's Republic of China. allanzhh@sohu.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002004:Brucella Vaccine / Q000235:genetics / Q000276:immunology*; D017347:Brucella melitensis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control; D002460:Cell Line; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010733:Phosphoglucomutase / Q000235:genetics* / Q000378:metabolism; D012756:Sheep; D014327:Trophoblasts / Q000276:immunology* / Q000382:microbiology; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D002004:Brucella Vaccine; D007376:Interleukin-2; D014613:Vaccines, Attenuated; D007371:Interferon-gamma; D010733:Phosphoglucomutase
keywords: B. melitensis M5-90; Live attenuated vaccines; PGM