title: Design of Polyepitope DNA Vaccine against Breast Carcinoma Cells and Analysis of Its Expression in Dendritic Cells.
abstract: Polyepitope DNA vaccine inducing T-cell-mediated immune response against cancer-specific antigens is a promising tool for selective elimination of tumor cells. Breast cancer-specific polyepitope DNA vaccine was designed using TEpredict and PolyCTLDesigner software on the basis of immunogenic peptides of HER2 and Mammaglobin-1 (Mam) tumor antigens. LPS-free preparations of plasmid DNA encoding polyepitope T-cell antigen and full-length copies of HER2 and Mam antigens were obtained. TaqMan-PCR systems for evaluation of the expression of immunogens in cells were created. The protocol of vaccine DNA delivery into dendritic cells was optimized. Expression of the target immunogens in dendritic cells derived from human peripheral blood mononuclear fraction after transfection with plasmid DNA preparations is demonstrated.
authors.affiliation: Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. zha_naz@niboch.nsc.ru.|E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Novosibirsk, Russia. zha_naz@niboch.nsc.ru.
authors.forename: Zh K
authors.initials: ZhK
authors.lastname: Nazarkina
authors.affiliation: Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Khar'kova
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Antonets
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Morozkin
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Russia.
authors.forename: S I
authors.initials: SI
authors.lastname: Bazhan
authors.affiliation: Vector State Research Center of Virology and Biotechnology, Koltsovo, Russia.
authors.forename: L I
authors.initials: LI
authors.lastname: Karpenko
authors.affiliation: Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Vlasov
authors.affiliation: Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.|Vector State Research Center of Virology and Biotechnology, Koltsovo, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Ilyichev
authors.affiliation: Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.|Vector State Research Center of Virology and Biotechnology, Koltsovo, Russia.
authors.forename: P P
authors.initials: PP
authors.lastname: Laktionov
mesh_terms: D001943:Breast Neoplasms / Q000276:immunology* / Q000517:prevention & control; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D060785:Mammaglobin A / Q000276:immunology*; D016133:Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D016753:Interleukin-10; D015850:Interleukin-6; D060785:Mammaglobin A; D018719:Erb-b2 Receptor Tyrosine Kinases; D011948:Receptors, Antigen, T-Cell; D019444:Vaccines, DNA; C508609:IL10 protein, human; C508600:IL6 protein, human; C556448:SCGB2A2 protein, human; C508053:ERBB2 protein, human
keywords: breast cancer; dendritic cells; polyepitope DNA vaccine