title: Generation of cytotoxic molecules and oxidative stress in haemolymph of pebrinised tasar silkworm Antheraea mylitta Drury.
abstract: The present study was carried out to investigate the effects of microsporidial infection on redox regulation mechanism and oxidative stress in tasar silkworm Antheraea mylitta. High level of superoxide radical (p < 0.05), nitric oxide (p < 0.001) and lipid peroxidation (p < 0.001) was observed in haemolymph of pebrinised larvae, which indicated the resultant generation of cytotoxic molecules and oxidative damage. Increased phenol oxidase (PO) activity in haemolymph of pebrinised larvae indicated the activation of immune defences during pathological conditions. In addition, higher level of glutathione-S-tranferase (GST) activity and reduced glutathione (GSH) level observed in pebrinised larvae indicated adaptive behaviour of tissue against toxic oxyradicals (p < 0.05). Conversely, low level of ascorbic acid (ASA) content suggested that the larvae might have used these compounds to counteract stress in tissues or low uptake under microspridial infection (p < 0.05). Present findings provide new insights into the cellular and biochemical bases of host-pathogen interactions in tasar silkworm A. mylitta.
authors.forename: Karmabeer
authors.initials: K
authors.lastname: Jena
authors.forename: Jay Prakash
authors.initials: JP
authors.lastname: Pandey
authors.forename: Anshu
authors.initials: A
authors.lastname: Priya
authors.forename: Papri
authors.initials: P
authors.lastname: Kundu
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Sinha
authors.forename: Harendra
authors.initials: H
authors.lastname: Yadav
authors.forename: Alok
authors.initials: A
authors.lastname: Sahay
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry / Q000378:metabolism; D006458:Hemolymph / Q000737:chemistry* / Q000378:metabolism; D008054:Lipid Peroxides / Q000737:chemistry / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism; D009036:Moths / Q000378:metabolism*; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism
chemical_list: D008054:Lipid Peroxides; D009569:Nitric Oxide; D014442:Monophenol Monooxygenase; D005982:Glutathione Transferase; D005978:Glutathione; D001205:Ascorbic Acid; D010100:Oxygen