title: Investigation on the effects of the atmospheric pressure plasma on wound healing in diabetic rats.
abstract: It is estimated that 15 percent of individuals with diabetes mellitus suffer from diabetic ulcers worldwide. The aim of this study is to present a non-thermal atmospheric plasma treatment as a novel therapy for diabetic wounds. The plasma consists of ionized helium gas that is produced by a high-voltage (8 kV) and high-frequency (6 kHz) power supply. Diabetes was induced in rats via an intravascular injection of streptozotocin. The plasma was then introduced to artificial xerograph wounds in the rats for 10 minutes. Immunohistochemistry assays was performed to determine the level of transforming growth factor (TGF-β1) cytokine. The results showed a low healing rate in the diabetic wounds compared with the wound-healing rate in non-diabetic animals (P < 0.05). Moreover, the results noted that plasma enhanced the wound-healing rate in the non-diabetic rats (P < 0.05), and significant wound contraction occurred after the plasma treatment compared with untreated diabetic wounds (P < 0.05). Histological analyses revealed the formation of an epidermis layer, neovascularization and cell proliferation. The plasma treatment also resulted in the release of TGF-β1 cytokine from cells in the tissue medium. The findings of this study demonstrate the effect of plasma treatment for wound healing in diabetic rats.
authors.affiliation: Plasma Physics Research Center, Science and Research branch of Islamic Azad University, Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Fathollah
authors.affiliation: Laser and Plasma institute, Shahid Beheshti University, Tehran, Iran.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Mirpour
authors.affiliation: Skin and Stem cell Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Parvin
authors.initials: P
authors.lastname: Mansouri
authors.affiliation: Experimental Medicine Research Center, Tehran University of Medical Science, Tehran, Iran.|Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Dehpour
authors.affiliation: Plasma Physics Research Center, Science and Research branch of Islamic Azad University, Tehran, Iran.
authors.forename: Mahmood
authors.initials: M
authors.lastname: Ghoranneviss
authors.affiliation: Experimental Medicine Research Center, Tehran University of Medical Science, Tehran, Iran.|Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Rahimi
authors.affiliation: Department of Pathology, Razi Skin Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Safaie Naraghi
authors.affiliation: Kassir Dermatology, 8335 Walnut Hill Ln, #140, Dallas, TX 75231, USA.
authors.forename: Reza
authors.initials: R
authors.lastname: Chalangari
authors.affiliation: Kassir Dermatology, 8335 Walnut Hill Ln, #140, Dallas, TX 75231, USA.
authors.forename: Katalin Martits
authors.initials: KM
authors.lastname: Chalangari
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D015870:Gene Expression; D006371:Helium / Q000494:pharmacology*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D058626:Plasma Gases / Q000494:pharmacology*; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013311:Streptozocin; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D014949:Wounds, Nonpenetrating / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D015415:Biomarkers; D058626:Plasma Gases; D053773:Transforming Growth Factor beta1; D006371:Helium; D013311:Streptozocin; D003094:Collagen