title: Detection and Phenotypic Characterization of Adult Neurogenesis.
abstract: Studies of adult neurogenesis have greatly expanded in the last decade, largely as a result of improved tools for detecting and quantifying neurogenesis. In this review, we summarize and critically evaluate detection methods for neurogenesis in mammalian and human brain tissue. Besides thymidine analog labeling, cell-cycle markers are discussed, as well as cell stage and lineage commitment markers. Use of these histological tools is critically evaluated in terms of their strengths and limitations, as well as possible artifacts. Finally, we discuss the method of radiocarbon dating for determining cell and tissue turnover in humans.
authors.affiliation: Institute for Neuroscience and Physiology, University of Gothenburg, Gothenburg, SE-405 30, Sweden.
authors.forename: H Georg
authors.initials: HG
authors.lastname: Kuhn
authors.affiliation: Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9070.
authors.forename: Amelia J
authors.initials: AJ
authors.lastname: Eisch
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm SE-171 77, Sweden.
authors.forename: Kirsty
authors.initials: K
authors.lastname: Spalding
authors.affiliation: Center for Stem Cell and Regenerative Medicine, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois 60064.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Peterson
mesh_terms: D000367:Age Factors; D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D019070:Cell Lineage; D005822:Genetic Vectors; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D055495:Neurogenesis; D010641:Phenotype; D055110:Radiometric Dating; D013194:Staining and Labeling / Q000379:methods; D013936:Thymidine / Q000031:analogs & derivatives
chemical_list: D015415:Biomarkers; D018797:Cell Cycle Proteins; D013936:Thymidine