title: Enhanced Osteogenic Activity of TiO2 Nanorod Films with Microscaled Distribution of Zn-CaP.
abstract: The topography at the micro-/nanoscale level and bioactive composition of material surfaces have been thought to play vital roles in their interactions with cells. However, it is still a challenge to further modify special topography with biodegradable composition or vice versa. In this study, TiO2 nanorod films covered with microscale-distributed Zn-containing calcium phosphate (Zn-CaP) were prepared, trying to create a micro-/nanoscale topography and Zn(2+) release capability. MC3T3-E1 cells cultured on TiO2 nanorod film with sparsely distributed Zn-CaP (TiO2/S-ZCP) had significantly higher biological responses than those on the films with densely distributed Zn-CaP (TiO2/D-ZCP) and fully covered Zn-CaP (F-ZCP). TiO2/S-ZCP film was demonstrated to facilitate osteogenic differentiation much more strongly than F-ZCP and TiO2/D-ZCP films based on evaluations of ALP, related gene expressions, and extracellular matrix mineralization. The higher osteogenic differentiation on TiO2/S-ZCP film is ascribed to the micro-/nanoscale topography from Zn-CaP coverage promoting cell adhesion and filopodia extension, and inducing differentiation-orientation in the initial stage. And consequently Zn(2+) release results in enhancement of differentiation. Therefore, we believe that better organization of the micro-/nanotopography and bioactive ion release on the surface would be a promising way to enhance osteogenic activity for orthopedic and dental implants.
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University , Hangzhou 310027, China.
authors.forename: Meng
authors.initials: M
authors.lastname: He
authors.affiliation: The Affiliated Stomatology Hospital of Medical College, Zhejiang University , Hangzhou 310003, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University , Hangzhou 310027, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University , Hangzhou 310027, China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.affiliation: The Affiliated Stomatology Hospital of Medical College, Zhejiang University , Hangzhou 310003, China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D008567:Membranes, Artificial; D051379:Mice; D043942:Nanotubes / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000943:Antigens, Differentiation; D002130:Calcium Phosphates; D008567:Membranes, Artificial; C009495:titanium dioxide; C020243:calcium phosphate; D014025:Titanium; D015032:Zinc
keywords: TiO2 nanorods; calcium phosphate; microtopography; nanotopography; osteogenesis; zinc