title: Nanosecond pulsed platelet-rich plasma (nsPRP) improves mechanical and electrical cardiac function following myocardial reperfusion injury.
abstract: Ischemia and reperfusion (I/R) of the heart is associated with biochemical and ionic changes that result in cardiac contractile and electrical dysfunction. In rabbits, platelet-rich plasma activated using nanosecond pulsed electric fields (nsPRP) has been shown to improve left ventricular pumping. Here, we demonstrate that nsPRP causes a similar improvement in mouse left ventricular function. We also show that nsPRP injection recovers electrical activity even before reperfusion begins. To uncover the mechanism of nsPRP action, we studied whether the enhanced left ventricular function in nsPRP rabbit and mouse hearts was associated with increased expression of heat-shock proteins and altered mitochondrial function under conditions of oxidative stress. Mouse hearts underwent 30 min of global ischemia and 1 h of reperfusion in situ. Rabbit hearts underwent 30 min of ischemia in vivo and were reperfused for 14 days. Hearts treated with nsPRP expressed significantly higher levels of Hsp27 and Hsp70 compared to hearts treated with vehicle. Also, pretreatment of cultured H9c2 cells with nsPRP significantly enhanced the "spare respiratory capacity (SRC)" also referred to as "respiratory reserve capacity" and ATP production in response to the uncoupler FCCP. These results suggest a cardioprotective effect of nsPRP on the ischemic heart during reperfusion.
authors.affiliation: Department of Medical Diagnostics and Translational Science, Old Dominion University, Norfolk, Virginia Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, Virginia bhargrav@odu.edu.
authors.forename: Barbara
authors.initials: B
authors.lastname: Hargrave
authors.affiliation: Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, Virginia Department of Electrical Engineering, Old Dominion University, Norfolk, Virginia.
authors.forename: Frency
authors.initials: F
authors.lastname: Varghese
authors.affiliation: Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, Virginia.
authors.forename: Nektarios
authors.initials: N
authors.lastname: Barabutis
authors.affiliation: Department of Medical Diagnostics and Translational Science, Old Dominion University, Norfolk, Virginia Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, Virginia.
authors.forename: John
authors.initials: J
authors.lastname: Catravas
authors.affiliation: Frank Reidy Center for Bioelectrics, Old Dominion University, Norfolk, Virginia Department of Electrical Engineering, Old Dominion University, Norfolk, Virginia.
authors.forename: Christian
authors.initials: C
authors.lastname: Zemlin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004558:Electric Stimulation; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D000067268:Isolated Heart Preparation; D051379:Mice; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D015539:Platelet Activation; D053657:Platelet-Rich Plasma / Q000528:radiation effects; D011817:Rabbits; D051381:Rats; D014162:Transfection; D016277:Ventricular Function, Left / Q000502:physiology*
chemical_list: D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins
keywords: Heat‐shock proteins; ischemic–reperfusion injury; mitochondria; platelet‐rich plasma; spare respiratory capacity