title: Sweet characterisation of prostate specific antigen using electrochemical lectin-based immunosensor assay and MALDI TOF/TOF analysis: Focus on sialic acid.
abstract: The construction of a sensitive electrochemical lectin-based immunosensor for detection of a prostate specific antigen (PSA) is shown here. Three lectins with different carbohydrate specificities were used in this study to glycoprofile PSA, which is the most common biomarker for prostate cancer (PCa) diagnosis. The biosensor showed presence of α-L-fucose and α-(2,6)-linked terminal sialic acid within PSA´s glycan with high abundance, while only traces of α-(2,3)-linked terminal sialic acid were found. MALDI TOF/TOF mass spectrometry was applied to validate results obtained by the biosensor with a focus on determination of a type of sialic acid linkage by two methods. The first direct comparison of electrochemical immunosensor assay employing lectins for PSA glycoprofiling with mass spectrometric techniques is provided here and both methods show significant agreement. Thus, electrochemical lectin-based immunosensor has potential to be applied for prostate cancer diagnosis.
authors.affiliation: Department of Glycobiotechnology, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Dominika
authors.initials: D
authors.lastname: Pihikova
authors.affiliation: Center of Excellence for Glycomics, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Pakanova
authors.affiliation: Center of Excellence for Glycomics, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Nemcovic
authors.affiliation: Center of Excellence for Glycomics, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Barath
authors.affiliation: Department of Glycobiotechnology, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Stefan
authors.initials: S
authors.lastname: Belicky
authors.affiliation: Department of Glycobiotechnology, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Bertok
authors.affiliation: Centre for Advanced Materials, Qatar University, Doha, Qatar.
authors.forename: Peter
authors.initials: P
authors.lastname: Kasak
authors.affiliation: Center of Excellence for Glycomics, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Mucha
authors.affiliation: Department of Glycobiotechnology, Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Tkac
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D037102:Lectins / Q000737:chemistry; D057230:Limit of Detection; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D017430:Prostate-Specific Antigen / Q000032:analysis*; D011471:Prostatic Neoplasms / Q000175:diagnosis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D055910:Antibodies, Immobilized; D037102:Lectins; D017430:Prostate-Specific Antigen; D019158:N-Acetylneuraminic Acid
keywords: Glycan; Glycoproteomics; Impedimetric biosensor; Lectins; MALDI-TOF/TOF; Prostate specific antigen (PSA)