title: BPA-Induced Deregulation Of Epigenetic Patterns: Effects On Female Zebrafish Reproduction.
abstract: Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation of epigenetic mechanisms. To determine its effects on female reproductive physiology and investigate whether changes in the expression levels of genes related to reproduction are caused by histone modifications, BPA concentrations consistent with environmental exposure were administered to zebrafish for three weeks. Effects on oocyte growth and maturation, autophagy and apoptosis processes, histone modifications, and DNA methylation were assessed by Real-Time PCR (qPCR), histology, and chromatin immunoprecipitation combined with qPCR analysis (ChIP-qPCR). The results showed that 5 μg/L BPA down-regulated oocyte maturation-promoting signals, likely through changes in the chromatin structure mediated by histone modifications, and promoted apoptosis in mature follicles. These data indicate that the negative effects of BPA on the female reproductive system may be due to its upstream ability to deregulate epigenetic mechanism.
authors.affiliation: Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.|INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Santangeli
authors.affiliation: Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.|INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Maradonna
authors.affiliation: Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Gioacchini
authors.affiliation: Dipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, Via S. Maria di Costantinopoli 16, 80138 Napoli, Italy.
authors.forename: Gilda
authors.initials: G
authors.lastname: Cobellis
authors.affiliation: Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Chiara Carla
authors.initials: CC
authors.lastname: Piccinetti
authors.affiliation: Dipartimento di Biologia, Università di Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Dalla Valle
authors.affiliation: Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.|INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy.
authors.forename: Oliana
authors.initials: O
authors.lastname: Carnevali
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001343:Autophagy / Q000235:genetics; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D052244:Endocrine Disruptors / Q000494:pharmacology*; D044127:Epigenesis, Genetic / Q000187:drug effects*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D009866:Oogenesis / Q000235:genetics; D010636:Phenols / Q000494:pharmacology*; D012098:Reproduction / Q000187:drug effects* / Q000235:genetics*; D059467:Transcriptome; D015027:Zebrafish; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds