title: [Changes in gut microbiota and serum D-lactate level and correlation analysis in children with recurrent pneumonia].
abstract: OBJECTIVE
  To study the changes in gut microbiota and serum D-lactate level and their significance in children with recurrent pneumonia.
  
  
  METHODS
  The stool and blood samples were collected from 30 children with recurrent pneumonia (recurrent group), 30 children with acute pneumonia (acute group), and 15 children receiving surgical operation (surgery group). The 16S rRNA fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to determine the numbers of Bifidobacterium and Escherichia coli in stool samples, and the ratio between the logarithmic values of the numbers of Bifidobacterium and Escherichia coli (B/E value) was calculated. The serum D-lactate level was measured, and correlation analysis was performed.
  
  
  RESULTS
  The recurrent group had a significantly lower number of Bifidobacterium and a significantly lower B/E value than the acute group and the surgery group (P<0.05), as well as a significantly higher number of Escherichia coli than the surgery group (P<0.05). There was no significant difference in the number of Escherichia coli between the recurrent group and the acute group. The recurrent group had a significantly higher serum D-lactate level than the acute group and the surgery group (P<0.05). In the recurrent group, B/E value was negatively correlated with serum D-lactate level (r=-0.539, P<0.05).
  
  
  CONCLUSIONS
  Children with recurrent pneumonia may have biological and mechanical barrier damage in the intestinal mucosa.
authors.affiliation: Department of Pediatrics, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China. pengshaodh630@126.com.
authors.forename: Shao
authors.initials: S
authors.lastname: Peng
authors.forename: Tian-Hua
authors.initials: TH
authors.lastname: DU
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001644:Bifidobacterium / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D005260:Female; D000069196:Gastrointestinal Microbiome; D006801:Humans; D007773:Lactates / Q000097:blood*; D011014:Pneumonia / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D012008:Recurrence
chemical_list: D007773:Lactates