title: Homology modeling and assigned functional annotation of an uncharacterized antitoxin protein from Streptomyces xinghaiensis.
abstract: Streptomyces xinghaiensis is a Gram-positive, aerobic and non-motile bacterium. The bacterial genome is known. Therefore, it is of interest to study the uncharacterized proteins in the genome. An uncharacterized protein (gi|518540893|86 residues) in the genome was selected for a comprehensive computational sequence-structure-function analysis using available data and tools. Subcellular localization of the targeted protein with conserved residues and assigned secondary structures is documented. Sequence homology search against the protein data bank (PDB) and non-redundant GenBank proteins using BLASTp showed different homologous proteins with known antitoxin function. A homology model of the target protein was developed using a known template (PDB ID: 3CTO:A) with 62% sequence similarity in HHpred after assessment using programs PROCHECK and QMEAN6. The predicted active site using CASTp is analyzed for assigned anti-toxin function. This information finds specific utility in annotating the said uncharacterized protein in the bacterial genome.
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Arafat Rahman
authors.initials: AR
authors.lastname: Oany
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Md Shahabuddin
authors.initials: MS
authors.lastname: Ahmed
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Nasreen
authors.initials: N
authors.lastname: Jahan
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Md Abdul
authors.initials: MA
authors.lastname: Latif
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Shahin
authors.initials: S
authors.lastname: Mahmud
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Md Ahmed
authors.initials: MA
authors.lastname: Hossain
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh.
authors.forename: Fatema
authors.initials: F
authors.lastname: Akter
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Hasibul Haque
authors.initials: HH
authors.lastname: Rakib
authors.affiliation: Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.
authors.forename: Md Shariful
authors.initials: MS
authors.lastname: Islam
keywords: Streptomyces xinghaiensis; active-site residues; antitoxin; homology modeling; hypothetical protein; prediction