title: Strain improvement of Trichoderma viride for increased cellulase production by irradiation of electron and (12)C(6+)-ion beams.
abstract: OBJECTIVE
  To improve cellulase production and activity, Trichoderma viride GSICC 62010 was subjected to mutation involving irradiation with an electron beam and subsequently with a (12)C(6+)-ion beam.
  
  
  RESULTS
  Mutant CIT 626 was the most promising cellulase producer after preliminary and secondary screening. Soluble protein production and cellulase activities were increased mutifold. The optimum temperature, pH and culture time for the maximum cellulase production of the selected mutant were 35 °C, pH 5 and 6 days. The highest cellulase production was obtained using wheat bran. The prepared cellulases from T. viride CIT 626 had twice the hydrolytic performance with sawdust (83 %) than that from the parent strain (42.5 %). Furthermore, molecular studies demonstrated that there were some key mutation sites suggesting that some amino acid changes in the protein caused by base mutations had led to the enhanced cellulase production and activity.
  
  
  CONCLUSIONS
  Mutagenesis with electron and (12)C(6+)-ion beams could be developed as an effective tool for improvement of cellulase producing strains.
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Zhaozhou
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China. ilizhaozhou@126.com.
authors.forename: Xiujin
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Daomin
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Gao
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Yuze
authors.initials: Y
authors.lastname: Hou
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, NO. 263 Kaiyuan Avenue, Luolong District, Luoyang, 471023, People's Republic of China.
authors.forename: Songbiao
authors.initials: S
authors.lastname: Li
authors.affiliation: Lanzhou Institute of Animal Science and Veterinary Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou, 730050, People's Republic of China.|Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730050, People's Republic of China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Liang
mesh_terms: D002480:Cellulase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004043:Dietary Fiber; D004583:Electrons; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D016296:Mutagenesis; D009154:Mutation; D059645:Mutation Rate; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D004043:Dietary Fiber; D002480:Cellulase
keywords: 12C6+-ion beam irradiation; Cellulase; Electron beam irradiation; Mutation; Trichoderma viride