title: Exogenous S100A8 protein inhibits PDGF-induced migration of airway smooth muscle cells in a RAGE-dependent manner.
abstract: S100A8 is an important member of the S100 protein family, which is involved in intracellular and extracellular regulatory activities. We previously reported that the S100A8 protein was differentially expressed in the asthmatic respiratory tracts. To understand the potential role of S100A8 in asthma, we investigated the effect of recombinant S100A8 protein on the platelet-derived growth factor (PDGF)-induced migration of airway smooth muscle cells (ASMCs) and the underlying molecular mechanism by using multiple methods, such as impedance-based xCELLigence migration assay, transwell migration assays and wound-healing assays. We found that exogenous S100A8 protein significantly inhibited PDGF-induced ASMC migration. Furthermore, the migration inhibition effect of S100A8 was blocked by neutralizing antibody against the receptor for advanced glycation end-products (RAGE), a potential receptor for the S100A8 protein. These findings provide direct evidence that exogenous S100A8 protein inhibits the PDGF-induced migration of ASMCs through the membrane receptor RAGE. Our study highlights a novel role of S100A8 as a potential means of counteracting airway remodeling in chronic airway diseases.
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Xu
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Lei-Miao
authors.initials: LM
authors.lastname: Yin
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Gyoung-Hee
authors.initials: GH
authors.lastname: Park
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Shanghai Research Institute of Acupuncture and Meridian, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address: yyq@shutcm.edu.cn.
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing; D001249:Asthma / Q000473:pathology / Q000503:physiopathology; D040501:Calgranulin A / Q000008:administration & dosage / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000502:physiology*; D051381:Rats; D000067759:Receptor for Advanced Glycation End Products / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D014132:Trachea / Q000473:pathology* / Q000502:physiology*; D014945:Wound Healing
chemical_list: C000593874:Ager protein, rat; D057134:Antibodies, Neutralizing; D040501:Calgranulin A; D010982:Platelet-Derived Growth Factor; D000067759:Receptor for Advanced Glycation End Products; D011994:Recombinant Proteins; C075278:platelet-derived growth factor A
keywords: Airway smooth muscle cells; Cell migration; Platelet-derived growth factor; S100A8; The receptor for advanced glycation end-products