title: New acyclic bis phenylpropanoid and neolignans, from Myristica fragrans Houtt., exhibiting PARP-1 and NF-κB inhibitory effects.
abstract: The bioassay-guided fractionation of the aril of Myristica fragrans (mace spice) yielded five phenolic compounds, one new acyclic bis phenylpropanoid (1) and four previously known phenolic compounds: compounds (1) (S) 1-(3,4,5-trimethoxyphenyl)-2-(3-methoxy-5-(prop-1-yl) phenyl)-propan-1-ol, (2) benzenemethanol; α-[1-[2,6-dimethoxy-4-(2-propen-1-yl)phenoxy]ethyl]-3,4-dimethoxy-1-acetate, (3) odoratisol A, phenol, 4-[(2S,3S)-2,3-dihydro-7-methoxy-3-methyl-5-(1E)-1-propenyl-2-benzofuranyl]-2,6-dimethoxy, (4) 1,3-benzodioxate-5-methanol,α-[1-[2,6-dimethoxy-4-(2-propenyl)phenoxy]ethyl]-acetate, (5) licarin C; benzofuran,2,3-dihydro-7-methoxy-3-methyl-5-(1E)-1-yl-2-(3,4,5-trimethoxyphenyl). An NMR tube Mosher ester reaction was used in an approach to characterize and determine the assignment of the absolute configuration of the new isolated chiral alcohol (1). The PARP-1 inhibitory activity was evaluated for compound (1) (IC50=3.04μM), compound (2) (IC50=0.001μM), compound (4) (IC50=22.07μM) and compound (5) (IC50=3.11μM). Furthermore, the isolated secondary metabolites were tested for NF-κB and K-Ras inhibitory activities. When tested in the p65 assay, compounds (2) and (4) displayed potent NF-κB inhibition (IC50=1.5 nM and 3.4nM, respectively).
authors.affiliation: Division of Pharmacy Practice and Science, College of Pharmacy, The Ohio State University, 141N Parks Hall, 500 W. 12th Avenue, Columbus, OH 43210, United States; Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, 141N Parks Hall, 500 W. 12th Avenue, Columbus, OH 43210, United States.
authors.forename: Ulyana
authors.initials: U
authors.lastname: Muñoz Acuña
authors.affiliation: Division of Pharmacy Practice and Science, College of Pharmacy, The Ohio State University, 141N Parks Hall, 500 W. 12th Avenue, Columbus, OH 43210, United States.
authors.forename: Peter J Blanco
authors.initials: PJ
authors.lastname: Carcache
authors.affiliation: Division of Pharmacy Practice and Science, College of Pharmacy, The Ohio State University, 141N Parks Hall, 500 W. 12th Avenue, Columbus, OH 43210, United States.
authors.forename: Susan
authors.initials: S
authors.lastname: Matthew
authors.affiliation: Division of Pharmacy Practice and Science, College of Pharmacy, The Ohio State University, 141N Parks Hall, 500 W. 12th Avenue, Columbus, OH 43210, United States; Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, 141N Parks Hall, 500 W. 12th Avenue, Columbus, OH 43210, United States. Electronic address: carcache-de-blan.1@osu.edu.
authors.forename: Esperanza J
authors.initials: EJ
authors.lastname: Carcache de Blanco
mesh_terms: D019073:HT29 Cells; D006801:Humans; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D026323:Myristica / Q000737:chemistry*; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D000067856:Poly(ADP-ribose) Polymerase Inhibitors / Q000494:pharmacology*
chemical_list: D017705:Lignans; D016328:NF-kappa B; D010636:Phenols; D000067856:Poly(ADP-ribose) Polymerase Inhibitors
keywords: Acyclic bisphenyl propanoids; K-Ras; Mace; Myristica fragrans; NF-κB; Neolignans; PARP-1