title: Plasmodium falciparum Bloom homologue, a nucleocytoplasmic protein, translocates in 3' to 5' direction and is essential for parasite growth.
abstract: Malaria caused by Plasmodium, particularly Plasmodium falciparum, is the most serious and widespread parasitic disease of humans. RecQ helicase family members are essential in homologous recombination-based error-free DNA repair processes in all domains of life. RecQ helicases present in each organism differ and several homologues have been identified in various multicellular organisms. These proteins are involved in various pathways of DNA metabolism by providing duplex unwinding function. Five members of RecQ family are present in Homo sapiens but P. falciparum contains only two members of this family. Here we report the detailed biochemical and functional characterization of the Bloom (Blm) homologue (PfBlm) from P. falciparum 3D7 strain. Purified PfBlm exhibits ATPase and 3' to 5' direction specific DNA helicase activity. The calculated average reaction rate of ATPase was ~13 pmol of ATP hydrolyzed/min/pmol of enzyme. The immunofluorescence assay results show that PfBlm is expressed in all the stages of intraerythrocytic development of the P. falciparum 3D7 strain. In some stages of development in addition to nucleus PfBlm also localizes in the cytoplasm. The gene disruption studies of PfBlm by dsRNA showed that it is required for the ex-vivo intraerythrocytic development of the parasite P. falciparum 3D7 strain. The dsRNA mediated inhibition of parasite growth suggests that a variety of pathways are affected resulting in curtailing of the parasite growth. This study will be helpful in unravelling the basic mechanism of DNA transaction in the malaria parasite and additionally it may provide leads to understand the parasite specific characteristics of this protein.
authors.affiliation: Malaria Group, International Centre for Genetic Engineering and Biotechnology, P. O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Farhana
authors.initials: F
authors.lastname: Rahman
authors.affiliation: Malaria Group, International Centre for Genetic Engineering and Biotechnology, P. O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Tarique
authors.affiliation: Malaria Group, International Centre for Genetic Engineering and Biotechnology, P. O. Box 10504, Aruna Asaf Ali Marg, New Delhi 110067, India. Electronic address: renu@icgeb.res.in.
authors.forename: Renu
authors.initials: R
authors.lastname: Tuteja
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics; D006801:Humans; D016778:Malaria, Falciparum / Q000201:enzymology* / Q000235:genetics / Q000469:parasitology; D010963:Plasmodium falciparum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000235:genetics; D053484:RecQ Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D012330:RNA, Double-Stranded; D000251:Adenosine Triphosphatases; C097151:Bloom syndrome protein; D053484:RecQ Helicases
keywords: ATPase; DNA helicase; Gene disruption; Malaria; Plasmodium falciparum; Repair