title: Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles.
abstract: RNA nanotechnology uses RNA structural motifs to build nanosized architectures that assemble through selective base-pair interactions. Herein, we report the crystal-structure-guided design of highly stable RNA nanotriangles that self-assemble cooperatively from short oligonucleotides. The crystal structure of an 81 nucleotide nanotriangle determined at 2.6 Å resolution reveals the so-far smallest circularly closed nanoobject made entirely of double-stranded RNA. The assembly of the nanotriangle architecture involved RNA corner motifs that were derived from ligand-responsive RNA switches, which offer the opportunity to control self-assembly and dissociation.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
authors.forename: Mark A
authors.identifier: http://orcid.org/0000-0003-0092-3203
authors.initials: MA
authors.lastname: Boerneke
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Dibrov
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA. tch@ucsd.edu.|Center for Drug Discovery Innovation, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA. tch@ucsd.edu.
authors.forename: Thomas
authors.identifier: http://orcid.org/0000-0002-9855-1593
authors.initials: T
authors.lastname: Hermann
mesh_terms: D018360:Crystallography, X-Ray; D049329:Nanostructures; D063347:Native Polyacrylamide Gel Electrophoresis; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
chemical_list: D012313:RNA
keywords: RNA structures; RNA switch; X-ray diffraction; nanostructures; self-assembly