title: DNA base-stacking assay utilizing catalytic hairpin assembly-induced gold nanoparticle aggregation for colorimetric protein sensing.
abstract: A label-free and enzyme-free colorimetric sensing platform for the amplified detection of fibronectin was developed based on an ingenious combination of catalytic hairpin assembly and a base stacking hybridization-based gold nanoparticle aggregation strategy. The detection limit of 2.3 pM is at least one order of magnitude lower than that of established fibronectin biosensors.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei 106, Taiwan, Republic of China. cwlinx@ntu.edu.tw.
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei 104, Taiwan, Republic of China. f122481@mmh.org.tw.
authors.forename: Chie-Pein
authors.initials: CP
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei 104, Taiwan, Republic of China. f122481@mmh.org.tw and Department of Medicine, Mackay Medical College, Taipei 252, Taiwan, Republic of China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei 106, Taiwan, Republic of China. cwlinx@ntu.edu.tw and Institute of Biomedical Electronic and Bioinformatics, National Taiwan University, Taipei 106, Taiwan, Republic of China and Center for Emerging Material and Advanced Devices, National Taiwan University, Taipei 106, Taiwan, Republic of China.
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles; D011506:Proteins / Q000032:analysis*
chemical_list: D011506:Proteins; D006046:Gold; D004247:DNA