title: McGISH identification and phenotypic description of leaf rust and yellow rust resistant partial amphiploids originating from a wheat × Thinopyrum synthetic hybrid cross.
abstract: A Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid wheatgrass is an excellent source of leaf and stem rust resistance produced by N.V.Tsitsin. Wheat line Mv9kr1 was crossed with this hybrid (Agropyron glael) in Hungary in order to transfer its advantageous agronomic traits into wheat. As the wheat parent was susceptible to leaf rust, the transfer of resistance was easily recognizable in the progenies. Three different partial amphiploid lines with leaf rust resistance were selected from the wheat/Thinopyrum hybrid derivatives by multicolour genomic in situ hybridization. Chromosome counting on the partial amphiploids revealed 58 chromosomes (18 wheatgrass) in line 194, 56 (14 wheatgrass) in line 195 and 54 (12 wheatgrass) in line 196. The wheat chromosomes present in these lines were identified and the wheatgrass chromosomes were characterized by fluorescence in situ hybridization using the repetitive DNA probes Afa-family, pSc119.2 and pTa71. The 3D wheat chromosome was missing from the lines. Molecular marker analysis showed the presence of the Lr24 leaf rust resistance gene in lines 195 and 196. The morphological traits were evaluated in the field during two consecutive seasons in two different locations.
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Kruppa
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.
authors.forename: Edina
authors.initials: E
authors.lastname: Türkösi
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.
authors.forename: Marianna
authors.initials: M
authors.lastname: Mayer
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.
authors.forename: Viola
authors.initials: V
authors.lastname: Tóth
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.
authors.forename: Gyula
authors.initials: G
authors.lastname: Vida
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Szakács
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, H-2462, Martonvásár, Brunszvik u. 2, Hungary. molnar.marta@agrar.mta.hu.
authors.forename: Márta
authors.initials: M
authors.lastname: Molnár-Láng
mesh_terms: D001487:Basidiomycota; D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics*; D005819:Genetic Markers; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D006109:Poaceae / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
chemical_list: D005819:Genetic Markers
keywords: FISH; Leaf rust resistance; Multicolour GISH; Partial amphiploid; Thinopyrum intermedium × Thinopyrum ponticum synthetic hybrid (Agropyron glael)