title: Evaluation of flagellum-related proteins FliD and FspA as subunit vaccines against Campylobacter jejuni colonisation in chickens.
abstract: Campylobacter is the leading cause of food-borne diarrhoea in humans in the developed world and consumption of contaminated poultry meat is the main source of infection. Vaccination of broilers could reduce carcass contamination and zoonotic infections. Towards this aim, we evaluated recombinant anti-Campylobacter subunit vaccines based on the flagellum-capping protein FliD and the flagellum-secreted protein FspA as they are immunogenic in chickens and the flagellum is vital for colonisation. In three studies, a recombinant FliD vaccine induced a transient but reproducible and statistically significant decrease of c. 2 log10 CFU/g in caecal colonisation levels at 49 days post-primary vaccination on the day of hatch. Levels of serum IgY specific to FliD positively correlated with caecal bacterial counts in individual birds, indicating that such antibodies may not play a role in protection. The data add to the limited repertoire of candidate antigens for the control of a key foodborne zoonosis.
authors.affiliation: The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK. Electronic address: Cosmin.Chintoan-Uta@roslin.ed.ac.uk.
authors.forename: C
authors.initials: C
authors.lastname: Chintoan-Uta
authors.affiliation: The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
authors.forename: R L
authors.initials: RL
authors.lastname: Cassady-Cain
authors.affiliation: The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
authors.forename: M P
authors.initials: MP
authors.lastname: Stevens
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000276:immunology; D026921:Amino Acid Transport Systems, Neutral / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D002169:Campylobacter Infections / Q000517:prevention & control / Q000662:veterinary*; D016123:Campylobacter jejuni; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D005407:Flagella / Q000276:immunology*; D007136:Immunoglobulins / Q000097:blood; D011201:Poultry Diseases / Q000517:prevention & control*; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D018528:ATP-Binding Cassette Transporters; D026921:Amino Acid Transport Systems, Neutral; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; C503090:CjaA protein, Campylobacter jejuni; C016512:IgY; D007136:Immunoglobulins; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; C076733:FlaD protein, Bacteria
keywords: Campylobacter jejuni; Chickens; Flagellar cap protein; Flagellum secreted protein; Subunit; Vaccine