title: Overexpression of an Arabidopsis heterogeneous nuclear ribonucleoprotein gene, AtRNP1, affects plant growth and reduces plant tolerance to drought and salt stresses.
abstract: Heterogeneous nuclear ribonucleoproteins (hnRNPs) participate in diverse regulations of plant growth and environmental stress responses. In this work, an Arabidopsis hnRNP of unknown function, AtRNP1, was investigated. We found that AtRNP1 gene is highly expressed in rosette and cauline leaves, and slightly induced under drought, salt, osmotic and ABA stresses. AtRNP1 protein is localized to both the nucleus and cytoplasm. We performed homologous overexpression of AtRNP1 and found that the transgenic plants showed shortened root length and plant height, and accelerated flowering. In addition, the transgenic plants also showed reduced tolerance to drought, salt, osmotic and ABA stresses. Further studies revealed that under both normal and stress conditions, the proline contents in the transgenic plants are markedly decreased, associated with reduced expression levels of a proline synthase gene and several stress-responsive genes. These results suggested that the overexpression of AtRNP1 negatively affects plant growth and abiotic stress tolerance.
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: wzy72609@163.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: xiuzh@psb.vib-ugent.be.
authors.forename: Xiuyang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: wangbing@ibcas.ac.cn.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: liuel14@lzu.edu.cn.
authors.forename: Erlong
authors.initials: E
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: 63710156@qq.com.
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address: wzhang1216@yahoo.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: hengliu@lzu.edu.cn.
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D030821:Plants, Genetically Modified / Q000502:physiology*; D012264:Ribonucleotide Reductases / Q000378:metabolism*; D055049:Salt Tolerance / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C000602038:RNR1 protein, Arabidopsis; D012264:Ribonucleotide Reductases
keywords: Abiotic stress; AtRNP1; Growth and development; Heterogeneous nuclear ribonucleoproteins; Proline