title: Putrescine protects hulless barley from damage due to UV-B stress via H2S- and H2O2-mediated signaling pathways.
abstract: In hulless barley, H 2 S mediated increases in H 2 O 2 induced by putrescine, and their interaction enhanced tolerance to UV-B by maintaining redox homeostasis and promoting the accumulation of UV-absorbing compounds. This study investigated the possible relationship between putrescence (Put), hydrogen sulfide (H2S) and hydrogen peroxide (H2O2) as well as the underlying mechanism of their interaction in reducing UV-B induced damage. UV-B radiation increased electrolyte leakage (EL) and the levels of malondialdehyde (MDA) and UV-absorbing compounds but reduced antioxidant enzyme activities and glutathione (GSH) and ascorbic acid (AsA) contents. Exogenous application of Put, H2S or H2O2 reduced some of the above-mentioned negative effects, but were enhanced by the addition of Put, H2S and H2O2 inhibitors. Moreover, the protective effect of Put against UV-B radiation-induced damage to hulless barley was diminished by DL-propargylglycine (PAG, a H2S biosynthesis inhibitor), hydroxylamine (HT, a H2S scavenger), diphenylene iodonium (DPI, a PM-NADPH oxidase inhibitor) and dimethylthiourea (DMTU, a ROS scavenger), and the effect of Put on H2O2 accumulation was abolished by HT. Taken together, as the downstream component of the Put signaling pathway, H2S mediated H2O2 accumulation, and H2O2 induced the accumulation of UV-absorbing compounds and maintained redox homeostasis under UV-B stress, thereby increasing the tolerance of hulless barley seedlings to UV-B stress.
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, China.|Tibetan Traditional Medical Hospital of Lhari, 18 South Renmin Road, Lhari, 852000, Nagchu, China.
authors.forename: Qien
authors.initials: Q
authors.lastname: Li
authors.affiliation: Life Science of College, Northwest Normal University, Lanzhou, 730070, Gansu, China.
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Biology, Qinghai University, 97 Ningzhang Road, Xining, 810016, China.
authors.forename: Yanning
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, China.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, China.
authors.forename: Shuaijun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, China.
authors.forename: Letao
authors.initials: L
authors.lastname: Bo
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, China.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Ding
authors.affiliation: School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, China. lizhean@lzu.edu.cn.
authors.forename: Lizhe
authors.initials: L
authors.lastname: An
mesh_terms: D000480:Alkynes / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006706:Homeostasis; D001467:Hordeum / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D019811:Hydroxylamine / Q000494:pharmacology; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D000480:Alkynes; D000975:Antioxidants; D004791:Enzyme Inhibitors; D009861:Onium Compounds; D010940:Plant Proteins; D020011:Protective Agents; D019811:Hydroxylamine; C009055:propargylglycine; C007517:diphenyleneiodonium; C038983:1,3-dimethylthiourea; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases; D005978:Glutathione; D013890:Thiourea; D001205:Ascorbic Acid; D005998:Glycine; D011700:Putrescine; D006862:Hydrogen Sulfide
keywords: Hulless barley; Hydrogen peroxide; Hydrogen sulfide; Oxidative stress; Putrescine; Redox homeostasis; UV-absorbing compounds