title: [Value of detecting bacterial 16S and 23S rRNA in interface membrane in diagnosis of periprosthetic joint infection].
abstract: OBJECTIVE
  To explore the value of detecting bacterial 16S rRNA with 23S rRNA in the diagnosis of periprosthetic joint infection (PJI).
  
  
  METHODS
  A prospective study was conducted among 67 patients with previous total hip arthroplasty (THA) undergoing a reoperation for infection (23 patients) or aseptic loosening (44 patients). Bacterial 16S rRNA and 23S rRNA in the interface membrane were detected by real-time PCR and their value in diagnosis of PJI was assessed.
  
  
  RESULTS
  The 16S rRNA and 23S rRNA showed no significant difference in their power in the diagnosis of PJI. The detection of 16S rRNA/23S rRNA showed a higher sensitivity and a greater negative predictive value in PJI diagnosis than the detection of 16S rRNA+23S rRNA (95.7% vs 52.2%, P<0.01; 97.6% vs 79.6%, P=0.01). The specificity, positive predictive value, and accuracy of the 4 diagnostic strategies were not significantly different.
  
  
  CONCLUSIONS
  The diagnostic power of 16S rRNA and 23S rRNA was similar in detecting PJI. Compared with the diagnostic strategy with 16S rRNA+23S rRNA, 16S rRNA/23S rRNA is more sensitive in detecting PJI.
authors.affiliation: Department of Orthopedics, Xiangya Hospital, Central South University, Hunan 410008, China. E-mail: ansultan@163.com.
authors.forename: Sen-Bo
authors.initials: SB
authors.lastname: An
authors.forename: Peng-de
authors.initials: PD
authors.lastname: Cai
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Yi-He
authors.initials: YH
authors.lastname: Hu
mesh_terms: D001170:Arthritis, Infectious / Q000175:diagnosis* / Q000382:microbiology; D019644:Arthroplasty, Replacement, Hip; D006801:Humans; D011446:Prospective Studies; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000382:microbiology; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification*; D012338:RNA, Ribosomal, 23S / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D012086:Reoperation; D012680:Sensitivity and Specificity
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S