title: DNAzyme-Controlled Cleavage of Dimer and Trimer Origami Tiles.
abstract: Dimers of origami tiles are bridged by the Pb(2+)-dependent DNAzyme sequence and its substrate or by the histidine-dependent DNAzyme sequence and its substrate to yield the dimers T1-T2 and T3-T4, respectively. The dimers are cleaved to monomer tiles in the presence of Pb(2+)-ions or histidine as triggers. Similarly, trimers of origami tiles are constructed by bridging the tiles with the Pb(2+)-ion-dependent DNAzyme sequence and the histidine-dependent DNAzyme sequence and their substrates yielding the trimer T1-T5-T4. In the presence of Pb(2+)-ions and/or histidine as triggers, the programmed cleavage of trimer proceeds. Using Pb(2+) or histidine as trigger cleaves the trimer to yield T5-T4 and T1 or the dimer T1-T5 and T4, respectively. In the presence of Pb(2+)-ions and histidine as triggers, the cleavage products are the monomer tiles T1, T5, and T4. The different cleavage products are identified by labeling the tiles with 0, 1, or 2 streptavidin labels and AFM imaging.
authors.affiliation: Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.affiliation: Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D021881:DNA, Catalytic / Q000737:chemistry*; D007854:Lead / Q000737:chemistry*; D009690:Nucleic Acid Conformation
chemical_list: D021881:DNA, Catalytic; D007854:Lead
keywords: AFM; DNA; Pb2+-ion; histidine; logic gate; nanotechnology