title: Anti-inflammatory effects of Lactobacillus brevis K65 on RAW 264.7 cells and in mice with dextran sulphate sodium-induced ulcerative colitis.
abstract: Lactic acid bacteria (LAB) with anti-inflammatory effects may be beneficial to the prevention or treatment for inflammation-related diseases, such as inflammatory bowel diseases. In an in vitro assay, heat-killed Lactobacillus brevis K65 (K65) reduced lipopolysaccharide-induced production of nitric oxide, tumour necrosis factor (TNF)-α and prostaglandin E2 in RAW 264.7 cells. In RAW 264.7 cells stably expressing an ind=ucible nitric oxide synthase (iNOS) reporter, viable K65 showed greater inhibition of iNOS production than its heat-killed form. In order to further examine the in vivo anti-inflammatory effect of K65, viable K65 was orally administered to BALB/c mice before and during the period of dextran sulphate sodium (DSS)-induced ulcerative colitis (UC). K65 improved UC symptoms, including reduced the levels of the pro-inflammatory cytokines, TNF-α, interleukin (IL)-6 and IL-1β, and lowered the activity of myeloperoxidase. Furthermore, K65 inhibited TNF-α, cyclo-oxygenase 2, forkhead box P3, and Toll-like receptor 4 mRNA expression in the colonic tissue of DSS-induced UC mice. Taken together, K65, a LAB with in vitro anti-inflammatory activity showed preventive effects on mice with DSS-induced UC by lowering the expression of inflammatory molecules.
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Sec. 2, Linong Street, 11221 Taipei City, Taiwan, ROC.
authors.forename: Y-W
authors.initials: YW
authors.lastname: Liu
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Sec. 2, Linong Street, 11221 Taipei City, Taiwan, ROC.
authors.forename: W-K
authors.initials: WK
authors.lastname: Ong
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Sec. 2, Linong Street, 11221 Taipei City, Taiwan, ROC.
authors.forename: Y-W
authors.initials: YW
authors.lastname: Su
authors.affiliation: 2 Bened Biomedicals Co., Ltd., 2F.-2, No.129, Sec. 2, Zhongshan N. Rd., Zhongshan Dist., 10448 Taipei City, Taiwan, ROC.
authors.forename: C-C
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Sec. 2, Linong Street, 11221 Taipei City, Taiwan, ROC.
authors.forename: T-H
authors.initials: TH
authors.lastname: Cheng
authors.affiliation: 1 Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Sec. 2, Linong Street, 11221 Taipei City, Taiwan, ROC.
authors.forename: Y-C
authors.initials: YC
authors.lastname: Tsai
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000473:pathology*; D016264:Dextran Sulfate / Q000633:toxicity*; D015232:Dinoprostone / Q000378:metabolism; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control*; D052196:Levilactobacillus brevis / Q000276:immunology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019001:Nitric Oxide Synthase / Q000378:metabolism; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; D019001:Nitric Oxide Synthase; D015232:Dinoprostone
keywords: Lactobacillus brevis K65; anti-inflammatory; dextran sulphate sodium; lactic acid bacteria; ulcerative colitis