title: Immunisation with proteins expressed during chronic murine melioidosis provides enhanced protection against disease.
abstract: There is an urgent need for an effective vaccine against human disease caused by Burkholderia pseudomallei, and although a wide range of candidates have been tested in mice none provide high level protection. We considered this might reflect the inability of these vaccine candidates to protect against chronic disease. Using Q-RT PCR we have identified 6 genes which are expressed in bacteria colonising spleens and lungs of chronically infected mice. Three of the genes (BPSL1897, BPSL3369 and BPSL2287) have been expressed in Escherichia coli and the encoded proteins purified. We have also included BPSL2765, a protein known to induce immune responses associated with a reduced incidence of chronic/recurrent disease in humans. Immunisation of mice with a combination of these antigens resulted in the induction of antibody responses against all of the proteins. Compared with mice immunised with capsular polysaccharide or LolC protein, mice immunised with the combination of chronic stage antigens showed enhanced protection against experimental disease in mice.
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: Olivia L
authors.initials: OL
authors.lastname: Champion
authors.affiliation: Department of Biosciences, University of Milan, Milan 20133, Italy.
authors.forename: Louise J
authors.initials: LJ
authors.lastname: Gourlay
authors.affiliation: Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, UK.
authors.forename: Andrew E
authors.initials: AE
authors.lastname: Scott
authors.affiliation: Department of Biosciences, University of Milan, Milan 20133, Italy.
authors.forename: Patricia
authors.initials: P
authors.lastname: Lassaux
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppler Street, London WC1E 7HT, UK.
authors.forename: Laura
authors.initials: L
authors.lastname: Conejero
authors.affiliation: Department of Biosciences, University of Milan, Milan 20133, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Perletti
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: Claudia
authors.initials: C
authors.lastname: Hemsley
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK; Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire, UK.
authors.forename: Joann
authors.initials: J
authors.lastname: Prior
authors.affiliation: London School of Hygiene and Tropical Medicine, Keppler Street, London WC1E 7HT, UK.
authors.forename: Gregory
authors.initials: G
authors.lastname: Bancroft
authors.affiliation: Department of Biosciences, University of Milan, Milan 20133, Italy; Consiglio Nazionale delle Ricerche, Institute of Biophysics, University of Milan, Milan 20133, Italy.
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK. Electronic address: r.w.titball@exeter.ac.uk.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000382:microbiology; D008554:Melioidosis / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000382:microbiology; D059467:Transcriptome
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G
keywords: Burkholderia pseudomallei; Chronic; Melioidosis; Vaccine