title: Naringin Protects Ovalbumin-Induced Airway Inflammation in a Mouse Model of Asthma.
abstract: Many plant species containing flavonoids have been widely used in traditional Chinese medicine. Naringin, a well-known flavanone glycoside of citrus fruits, possesses antioxidant, anti-inflammatory, anti-apoptotic, anti-ulcer, anti-osteoporosis, and anti-carcinogenic properties. The aim of the study was to investigate the anti-asthmatic effects of naringin and the possible mechanisms. Asthma model was established by ovalbumin. A total of 50 mice were randomly assigned to five experimental groups: control, model, and dexamethasone (2 mg/kg, orally) and naringin (5 mg/kg, 10 mg/kg, orally). Airway resistance (Raw) were measured, histological studies were evaluated by the hematoxylin and eosin (HE) staining, OVA-specific serum and BALF IgE levels and Th1/Th2 cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA), and Th1/Th2 cells was evaluated by flow cytometry (FCM). T-bet and GABA3 in the lung were evaluated by Western blot. Our study demonstrated that naringin inhibited OVA-induced increases in Raw and eosinophil count; OVA-induced effects on interleukin (IL)-4 and INF-gamma levels were blunted with naringin administration. Histological studies demonstrated that naringin substantially inhibited OVA-induced eosinophilia in lung tissue and airway tissue. Flow cytometry studies demonstrated that naringin substantially inhibited Th2 cells and enhanced Th1 cells. Naringin substantially inhibited GABA3 and increased T-bet. These findings suggest that naringin may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.
authors.affiliation: Changchun University of Chinese Medicine, No.1478 Gongnong Road, Changchun City, Jilin Province, 130021, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Guihua
authors.affiliation: Changchun University of Chinese Medicine, No.1478 Gongnong Road, Changchun City, Jilin Province, 130021, China. liushuyinch@126.com.
authors.forename: Liu
authors.initials: L
authors.lastname: Shuyin
authors.affiliation: Changchun University of Chinese Medicine, No.1478 Gongnong Road, Changchun City, Jilin Province, 130021, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Jinliang
authors.affiliation: Changchun University of Chinese Medicine, No.1478 Gongnong Road, Changchun City, Jilin Province, 130021, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D044950:Flavanones / Q000627:therapeutic use*; D007073:Immunoglobulin E / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008168:Lung / Q000473:pathology; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D020825:T-Box Domain Proteins / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology; D058408:Th1-Th2 Balance / Q000187:drug effects*; D018418:Th2 Cells / Q000276:immunology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: D018927:Anti-Asthmatic Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D044950:Flavanones; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015847:Interleukin-4; D007073:Immunoglobulin E; D005680:gamma-Aminobutyric Acid; D003907:Dexamethasone; D007371:Interferon-gamma; D010047:Ovalbumin; C005274:naringin
keywords: Th1/Th2; asthma; naringin