title: [Tubacin promotes Foxp3 expression and suppressive function of mouse CD4+;CD25+; regulatory T cells].
abstract: OBJECTIVE
  To observe the changes of fork head transcription factor (Foxp3) expressions and suppressive functions of mouse regulatory T cells (Tregs) after stimulated by tubacin (histone deacetylase 6 inhibitor) in vitro.
  
  
  METHODS
  CD4(+) CD25(+) T and CD4(+) CD25(-) T cells were gained from C57BL/6J mouse spleen lymphocytes by double positive magnetic bead sorting method. Natural Tregs (nTregs) and transforming growth factor β1 (TGF-β1)-induced Tregs (iTregs) were stimulated by tubacin in vitro. Foxp3 expression of each group was identified by reverse transcription PCR, and immunosuppressive activities of each group were tested by the mixed lymphocyte culture (MLC).
  
  
  RESULTS
  Foxp3 expression of nTregs and iTregs were significantly enhanced after stimulated with tubacin. The tubacin induced CD4(+) CD25(+) Foxp3(high) Tregs significantly inhibited syngeneic CD4(+) CD25(-) T cell activation.
  
  
  CONCLUSIONS
  Tubacin can upregulate Foxp3 expression of CD4(+) CD25(+) Tregs and enhances their cellular immunosuppressive capability.
authors.affiliation: Department of Cardiac Surgery, Second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Cardiac Surgery, Second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China. *Corresponding author, E-mail: shtjdoctor@aliyun.com.
authors.forename: Ziying
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Cardiac Surgery, Second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Cardiac Surgery, Second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Cardiac Surgery, Second Affiliated Hospital, Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000813:Anilides / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D000813:Anilides; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D053645:Interleukin-2 Receptor alpha Subunit; D053773:Transforming Growth Factor beta1; C474316:tubacin