title: [Study on biomimetic mineralization of lipopolysaccharide-amine nanopolymersomes/hyaluronic acid polyelectrolyte films on titanium surface].
abstract: OBJECTIVE
  To explore biomimetic mineralization of polyelectrolyte multilayer films (PEM) of gene-loaded lipopolysaccharide-amine nanopolymersomes/hyaluronic acid self assembled on titanium surface.
  
  
  METHODS
  Via lay-by-layer self assembly technology, PEM were constructed on titanium or quartz surface using bone morphogenetic protein-2(BMP-2) plasmid-loaded lipopolysaccharide-amine nanopolymersomes(pLNP) as a polycation, and hyaluronic acid(HA) as a polyanion. The constructed PEM were defined as substrate-pLNP-(HA-pLNP)n, where a successive deposition of HA and pLNP on substrate surface was defined as one assembly cycle, and n was the cycle number. Biomimetic mineralization on surfaces of Ti-pLNP-(HA-pLNP)4(Group A, with outermost layer of pLNP), Ti-pLNP-(HA-pLNP)4.5(Group B, with outermost layer of HA), blank control(polished titanium, Ti) and alkaline-heat treated titanium(Ti-OH) were investigated. The biomimetic mineralization was analyzed by observing the topography under field-emisssion electron microscopy(FE-SEM), characterizing the surface chemical structure and components via X-ray diffractometer(XRD) and X-ray energy disperse spectroscopy(EDS).
  
  
  RESULTS
  For experiment groups, XRD analysis showed that the diffraction peak of hydroxyapatite appeared, and its intensity was higher than that for Ti group. FE-SEM images showed that its surface was homogeneously covered by discrete agglomerate of big particles. EDS spectra showed that the percentage of Ca and P were 77.24% and 64.23%, and these were much higher than those in Ti group.
  
  
  CONCLUSIONS
  The surface of Ti-pLNP-(HA-pLNP)n is favorable for in vitro biomimetic mineralization.
authors.affiliation: Department of Prosthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Teng
authors.affiliation: Department of Prosthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors.forename: Xiangxia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Prosthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China.
authors.forename: Hongzhang
authors.initials: H
authors.lastname: Huang
mesh_terms: D000588:Amines / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2; D017886:Durapatite / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008070:Lipopolysaccharides; D053761:Nanocomposites / Q000737:chemistry; D010957:Plasmids; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000588:Amines; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D008070:Lipopolysaccharides; D006820:Hyaluronic Acid; D017886:Durapatite; D014025:Titanium