title: Anti-NDV activity of baicalin from a traditional Chinese medicine in vitro.
abstract: The purpose of this study was to investigate the anti-Newcastle disease virus (NDV) activities of baicalin from Scutellaria baicalensis, a Traditional Chinese Medicine in vitro. Chicken embryo fibroblasts (CEFs) were infected with NDV, and quantitative analysis of apoptotic cells was performed using flow cytometry. Cytotoxicity and anti-viral activities of baicalin were studied using the MTT method. The results showed that the maximal safe concentrations of baicalin to CEFs was 1 × 2(-2) mg/ml. Baicalin could directly kill NDV, inhibit the infectivity of NDV to CEF and block intracellular NDV. It inhibited the apoptosis of NDV-infected CEFs and suppressed the spread of NDV. These results indicate that baicalin has strong anti-NDV activity and has the potential for use as components of an antiviral drug.
authors.affiliation: Agricultural and Environmental Branch, Jiaxing Vocational Technical College, Zhejiang Province, Jiaxing 314036, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jia
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Xu
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Hu
authors.forename: Tianlong
authors.initials: T
authors.lastname: Zhu
authors.forename: Tingting
authors.initials: T
authors.lastname: Ma
authors.forename: Haihong
authors.initials: H
authors.lastname: Wu
authors.forename: Liwen
authors.initials: L
authors.lastname: Hu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002642:Chick Embryo / Q000821:virology; D005419:Flavonoids / Q000494:pharmacology*; D066298:In Vitro Techniques; D009521:Newcastle Disease / Q000178:diet therapy; D009522:Newcastle disease virus / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D005419:Flavonoids; C038044:baicalin