title: Molecular characterization of Capim and Enseada orthobunyaviruses.
abstract: Capim and Enseada viruses are members of the genus Orthobunyavirus isolated from mosquitoes and mammals in Brazil. Despite seroprevalence studies indicating human infections in Latin America, these viruses remain relatively unknown and unstudied. In order to better understand the genetic and evolutionary relationships among orthobunyaviruses, we sequenced the three genomic segments of Capim and Enseada orthobunyaviruses. Based on phylogenetic analysis, we demonstrated that these viruses depicted two new distinct clades, one represented by Enseada and another composed of Capim virus. In general, the genome organization and genetic traits of these viruses are similar to other orthobunyaviruses however, the open reading frame (ORF) of the putative nonstructural NSs protein of Enseada orthobunyavirus precedes the nucleocapsid ORF. Overall, our study provides details on the molecular characteristics of the prototype species of two groups within the Orthobunyavirus genus, revealing novel features into the genetic diversity and evolution of this genus.
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, Brazil. Electronic address: wmarciel@hotmail.com.
authors.forename: William Marciel
authors.initials: WM
authors.lastname: de Souza
authors.affiliation: Department of Cell and Molecular Biology, University of Sao Paulo School of Medicine, Ribeirão Preto, Brazil.
authors.forename: Gustavo Olszanski
authors.initials: GO
authors.lastname: Acrani
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, Brazil.
authors.forename: Marilia Farignoli
authors.initials: MF
authors.lastname: Romeiro
authors.affiliation: Laboratório Central de Tecnologias de Alto Desempenho em Ciências da Vida (LaCTAD), Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Reis
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, Brazil.
authors.forename: Aline Lavado
authors.initials: AL
authors.lastname: Tolardo
authors.affiliation: Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Brazil.
authors.forename: Sandro Patroca
authors.initials: SP
authors.lastname: da Silva
authors.affiliation: Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ananindeua, Brazil.
authors.forename: Daniele Barbosa
authors.initials: DB
authors.lastname: de Almeida Medeiros
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, UK.
authors.forename: Mariana
authors.initials: M
authors.lastname: Varela
authors.affiliation: Center for Technological Innovation, Instituto Evandro Chagas, Ananindeua, Brazil.
authors.forename: Márcio Roberto Teixeira
authors.initials: MRT
authors.lastname: Nunes
authors.affiliation: Virology Research Center, School of Medicine of Ribeirao Preto of University of São Paulo, Ribeirao Preto, Brazil.
authors.forename: Luiz Tadeu Moraes
authors.initials: LTM
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D001938:Brazil; D009033:Culicidae / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D008322:Mammals / Q000821:virology*; D019590:Nucleocapsid Proteins / Q000235:genetics; D016366:Open Reading Frames; D016851:Orthobunyavirus / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D017423:Sequence Analysis, RNA / Q000379:methods*; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D019590:Nucleocapsid Proteins; D017361:Viral Nonstructural Proteins
keywords: Arbovirus; Bunyavirus; Capim orthobunyavirus; Enseada orthobunyavirus; Molecular characterization; Orthobunyavirus