title: Molecular characterization and expression of metallothionein from freshwater pearl mussel, Hyriopsis schlegelii.
abstract: Two metallothionein genes (HsMT1 and HsMT2) were first identified and described from Hyriopsis schlegelii. The open reading frame of HsMT1 and HsMT2 were 216 and 222 bp, encoding a protein of 71 and 73 amino acid residues. The deduced amino acid sequences showed they contained parts of typical MT characteristics, apart from HsMT2 lacked Cys-Cys motifs. The phylogenetic tree showed HsMT1 shared a high similarity with that of other molluscs, but HsMT2 was split into a distinct group separated from known molluscan MTs. HsMT1 exhibited constitutive expression in all examined tissues and the highest expression occurred in hepatopancreas, however, nearly all HsMT2 was just detected in gonad. After Cd exposure, their mRNA levels presented similar expression patterns. The transgenic bacteria of HsMT1 showed higher tolerance than HsMT2 in Cd environment. It was implied that HsMT1 and HsMT2 were involved in metal response but HsMT2 might have other physiological functions.
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.
authors.forename: Chengyuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Sheng
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.|b Key Laboratory of Molecular Biology and Genetic Engineering of Jiangxi , Nanchang University , Jiangxi , China.
authors.forename: Yijiang
authors.initials: Y
authors.lastname: Hong
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.
authors.forename: Kou
authors.initials: K
authors.lastname: Peng
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Wang
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Shi
authors.affiliation: a College of Life Sciences , Nanchang University , Nanchang , China.
authors.forename: Beijuan
authors.initials: B
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005618:Fresh Water; D015870:Gene Expression; D006066:Gonads / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D020033:Protein Isoforms; D011994:Recombinant Proteins; D002104:Cadmium; D008668:Metallothionein
keywords: Hyriopsis schlegelii; metal response; metallothionein