title: [Development of colloidal gold immunochromatography assay strip for schistosomiasis diagnosis in domestic animals].
abstract: OBJECTIVE
  To develop a quick and easy colloidal gold immunochromatography assay (GICA) strip for schistosomiasis diagnosis in domestic animals.
  
  
  METHODS
  The reconstruction of Streptococcal Protein G (SPG) was designed and its gene was subcloned into plasmid pET-28a(+) to express in Escherichia coli. The recombinant SPG was purified and labeled with colloidal gold. The Schistosoma japonicum soluble egg antigen (SEA) and rSPG were blotted on the nitrocellulose membrane for the test line and control line respectively. The specificity, sensitivity and cross-reaction of the strip method were detected.
  
  
  RESULTS
  The rSPG was successfully expressed and purified to label with colloidal gold. The colloidal gold immunochromatography assay strips were assembled and they could detect the sera of S. japonicum infected BALB/c mice, New Zealand white rabbits, buffalo and sheep successfully. Besides, the sensitivity of GICA strip was 100% in the sera of mice and the serum of rabbits with S. japonicum infection. The specificity was 100% in the serum of mice and the sera of rabbits with free of infection. The sensitivity was 100% in the sera of sheep with miracidia of S. japonicum hatching from the stool and the specificity was 88.46% in the sera of sheep without that. The sensitivity was 94.44% in the sera of buffalo with miracidia hatching from the stool and the specificity was 100% in the sera of buffalo without that. The cross-reaction rate was 5.88% in Paramphistomum.
  
  
  CONCLUSIONS
  The GICA strip can successfully detect a variety of S. japonicum infected domestic animals and may be a useful tool for screening on a large scale in the endemic areas.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.forename: Deng-yun
authors.initials: DY
authors.lastname: Zhao
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Jiao-jiao
authors.initials: JJ
authors.lastname: Lin
authors.forename: Jin-ming
authors.initials: JM
authors.lastname: Liu
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Xu
authors.lastname: Zhu Chuan-gang
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002020:Buffaloes; D002846:Chromatography, Affinity / Q000379:methods* / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel; D017979:Gold Colloid / Q000737:chemistry / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology; D006790:Host-Parasite Interactions / Q000276:immunology; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011934:Reagent Strips; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012549:Schistosoma japonicum / Q000276:immunology* / Q000502:physiology; D012552:Schistosomiasis / Q000175:diagnosis / Q000276:immunology* / Q000662:veterinary; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology; D013045:Species Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D001426:Bacterial Proteins; D017979:Gold Colloid; D015801:Helminth Proteins; C034284:IgG Fc-binding protein, Streptococcus; D011934:Reagent Strips; D011994:Recombinant Proteins; C079442:gp50 protein, Schistosoma