title: Evaluation of the SediMax automated microscopy sediment analyzer and the Sysmex UF-1000i flow cytometer as screening tools to rule out negative urinary tract infections.
abstract: Urinary tract infections (UTI) are highly prevalent in nosocomial and community settings, and their diagnosis is costly and time-consuming. Screening methods represent an important advance towards the final UTI diagnosis, diminishing inappropriate treatment or clinical complications. Automated analyzers have been developed and commercialized to screen and rule out negative urine samples. The aim of this study was to evaluate two of these automated analyzers (SediMax, an automatic sediment analyzer and UF-1000i a flow cytometer) to predict negative urine cultures. A total of 1934 urine samples were analyzed. A very strong correlation for white blood cells (WBC) (rs: 0.928) and a strong correlation for bacteria (BAC) (rs: 0.693) were obtained. We also calculated optimal cut-off points for both autoanalyzers: 18 WBC/μL and 97 BAC/μL for SediMax (sensitivity=96.25%, specificity=63.04%, negative predictive value=97.97%), and 40 WBC/μL and 460 BAC/μL for UF-1000i (sensitivity=98.13%, specificity=79.16%, negative predictive value=99.18%). The use of SediMax and UF-1000i resulted in a 46.33% and 57.19% reduction of all samples cultured, respectively. In conclusion, both analyzers are good UTI screening tools in our setting.
authors.affiliation: Microbiology Service, Germans Trias i Pujol University Hospital, Department of Genetics and Microbiology, Autonomous University of Barcelona, Badalona, Spain. Electronic address: melania.inigo@gmail.com.
authors.forename: Melania
authors.initials: M
authors.lastname: Íñigo
authors.affiliation: Microbiology Service, Fundació Institut d'Investigació en Ciències de la Salut Germans Trias iPujol, Germans Trias i Pujol University Hospital, Autonomous University of Barcelona, Badalona, Spain.
authors.forename: Andreu
authors.initials: A
authors.lastname: Coello
authors.affiliation: Microbiology Service, Germans Trias i Pujol University Hospital, Department of Genetics and Microbiology, Autonomous University of Barcelona, Badalona, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Fernández-Rivas
authors.affiliation: Microbiology Service, Germans Trias i Pujol University Hospital, Department of Genetics and Microbiology, Autonomous University of Barcelona, Badalona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Carrasco
authors.affiliation: Microbiology Service, Germans Trias i Pujol University Hospital, Department of Genetics and Microbiology, Autonomous University of Barcelona, Badalona, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Marcó
authors.affiliation: Laboratori Barcelonès Nord i Vallès Oriental, Institut Català de la Salut, Badalona, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: Fernández
authors.affiliation: Laboratori Barcelonès Nord i Vallès Oriental, Institut Català de la Salut, Badalona, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Casamajor
authors.affiliation: Microbiology Service, Germans Trias i Pujol University Hospital, Department of Genetics and Microbiology, Autonomous University of Barcelona, Badalona, Spain; CIBER in Respiratory Diseases (CIBERES), Madrid, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Ausina
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007223:Infant; D057230:Limit of Detection; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D016482:Urinalysis / Q000379:methods*; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000652:urine*; D055815:Young Adult
keywords: Autoanalyzers; Automated urine sediment; Flow cytometry; Urinalysis; Urinary tract infection