title: Characterization at the individual cell level and in whole blood samples of shear stress preventing red blood cells aggregation.
abstract: The aggregation of red blood cells (RBC) is an intrinsic feature of blood that has a strong impact on its microcirculation. For a number of years it has been attracting a great attention in basic research and clinical studies. Here, we study a relationship between the RBC aggregation parameters measured at the individual cell level and in a whole blood sample. The home made optical tweezers were used to measure the aggregating and disaggregating forces for a pair of interacting RBCs, at the individual cell level, in order to evaluate the corresponding shear stresses. The RheoScan aggregometer was used for the measurements of critical shear stress (CSS) in whole blood samples. The correlation between CSS and the shear stress required to stop an RBC pair from aggregating was found. The shear stress required to disaggregate a pair of RBCs using the double channel optical tweezers appeared to be about 10 times higher than CSS. The correlation between shear stresses required to prevent RBCs from aggregation at the individual cell level and in whole blood samples was estimated and assessed quantitatively. The experimental approach developed has a high potential for advancing hemorheological studies.
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia; Opto-Electronics and Measurement Techniques Research Unit, University of Oulu, Oulu, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Lee
authors.affiliation: Opto-Electronics and Measurement Techniques Research Unit, University of Oulu, Oulu, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Kinnunen
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Danilina
authors.affiliation: Faculty of Computational Mathematics and Cybernetics, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: V D
authors.initials: VD
authors.lastname: Ustinov
authors.affiliation: School of Mechanical Engineering, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-713, South Korea. Electronic address: lexerdshin@korea.ac.kr.
authors.forename: S
authors.initials: S
authors.lastname: Shin
authors.affiliation: Opto-Electronics and Measurement Techniques Research Unit, University of Oulu, Oulu, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Meglinski
authors.affiliation: Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia; International Laser Center, Lomonosov Moscow State University, Moscow, Russia. Electronic address: avpriezz@gmail.com.
authors.forename: A V
authors.initials: AV
authors.lastname: Priezzhev
mesh_terms: D000328:Adult; D004903:Erythrocyte Aggregation; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D008297:Male; D052898:Optical Tweezers; D013314:Stress, Mechanical; D055815:Young Adult
keywords: Critical shear stress; Disaggregating shear stress; Optical tweezers; Red blood cell aggregating force; Red blood cell aggregation