title: [Preparation and characterization of polyclonal antibody against 5-fluorouracil].
abstract: OBJECTIVE
  To prepare 5-fluorouracil (5-FU) immunogen and develop polyclonal antibodies against 5-FU.
  
  
  METHODS
  The derivant of 5-FU (5-fluorouracil-1-yl-aceto amino acid, 5-FUAA) was synthesized, and then conjugated with bovine serum albumin (BSA) or ovalbumin (OVA) by carbodiimide (CDI) method. 5-FUAA conjugating BSA (5-FUAA-BSA) was used to immunize BALB/c mice to produce antiserum, and 5-FUAA conjugating OVA (5-FUAA-OVA) was used as coating antigen to detect the titer of the antiserum by indirect ELISA. Furthermore, specificity of the polyclonal antiserum was identified by ELISA and Western blotting.
  
  
  RESULTS
  5-FU derivant 5-FUAA was successfully synthesized and conjugated with BSA or OVA. Indirect ELISA showed that the titer of the antiserum from BALB/c mice immunized with 5-FUAA-BAS reached 1:1 280 000. Moreover, ELISA and Western blotting proved that the anti-serum could combine 5-FU specifically.
  
  
  CONCLUSIONS
  The experiment has prepared high-specific and high-titer polyclonal antibody against 5-FU.
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Yuchi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Youchao
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Shiyan
authors.initials: S
authors.lastname: Yu
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Junai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College, Dongguan 523808, China. *Corresponding author, E-mail: imxujunfa@163.com.
authors.forename: Junfa
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005472:Fluorouracil / Q000737:chemistry / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology*; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D005472:Fluorouracil