title: Hake fish bone as a calcium source for efficient bone mineralization.
abstract: Calcium is recognized as an essential nutritional factor for bone health. An adequate intake is important to achieve or maintain optimal bone mass in particular during growth and old age. The aim of the present study was to evaluate the efficiency of hake fish bone (HBF) as a calcium source for bone mineralization: in vitro on osteosarcoma SaOS-2 cells, cultured in Ca-free osteogenic medium (OM) and in vivo on young growing rats fed a low-calcium diet. Lithotame (L), a Ca supplement derived from Lithothamnium calcareum, was used as control. In vitro experiments showed that HBF supplementation provided bone mineralization similar to standard OM, whereas L supplementation showed lower activity. In vivo low-Ca HBF-added and L-added diet similarly affected bone deposition. Physico-chemical parameters concerning bone mineralization, such as femur breaking force, tibia density and calcium/phosphorus mineral content, had beneficial effects from both Ca supplementations, in the absence of any evident adverse effect. We conclude HBF derived from by-product from the fish industry is a good calcium supplier with comparable efficacy to L.
authors.affiliation: a Department of Pharmacy , University of Parma , Parma , Italy ;
authors.forename: Lisa
authors.initials: L
authors.lastname: Flammini
authors.affiliation: b Department of Food Science , University of Parma , Parma , Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Martuzzi
authors.affiliation: a Department of Pharmacy , University of Parma , Parma , Italy ;
authors.forename: Valentina
authors.initials: V
authors.lastname: Vivo
authors.affiliation: b Department of Food Science , University of Parma , Parma , Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Ghirri
authors.affiliation: a Department of Pharmacy , University of Parma , Parma , Italy ;
authors.forename: Enrico
authors.initials: E
authors.lastname: Salomi
authors.affiliation: b Department of Food Science , University of Parma , Parma , Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Bignetti
authors.affiliation: a Department of Pharmacy , University of Parma , Parma , Italy ;
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Barocelli
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005399:Fishes; D008297:Male; D012516:Osteosarcoma / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012624:Seaweed
chemical_list: D002118:Calcium
keywords: Calcium; diet supplement; hake bone flour; lithotame; phosphate