title: Ginsenoside Rg1 enhances lymphatic transport of intrapulmonary silica via VEGF-C/VEGFR-3 signaling in silicotic rats.
abstract: Ginsenoside Rg1, extracted mainly from Panax ginseng, has been shown to exert strong pro-angiogenic activities in vivo. But it is unclear whether ginsenoside Rg1 could promote lung lymphangiogenesis to improve lymphatic transport of intrapulmonary silica in silicotic rats. Here we investigated the effect of ginsenoside Rg1 on lymphatic transport of silica during experimental silicosis, and found that ginsenoside Rg1 treatment significantly raised the silicon content in tracheobronchial lymph nodes and serum to reduce the silicon level in lung interstitium, meanwhile increased pulmonary lymphatic vessel density by enhancing the protein and mRNA expressions of vascular endothelial growth factor-C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3). The stimulative effect of ginsenoside Rg1 on lymphatic transport of silica was actively correlated with its pro-lymphangiogenic identity. And VEGFR-3 inhibitor SAR131675 blocked these above effects of ginsenoside Rg1. These findings suggest that ginsenoside Rg1 exhibits good protective effect against lung burden of silica during experimental silicosis through improving lymphatic transport of intrapulmonary silica, which is potentially associated with the activation of VEGF-C/VEGFR-3 signaling pathway.
authors.affiliation: Department of Occupational Disease, Peking University Third Hospital, 49 North Garden Road, Beijing, 100191, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Occupational Disease, Peking University Third Hospital, 49 North Garden Road, Beijing, 100191, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Mao
authors.affiliation: Department of Occupational Disease, Peking University Third Hospital, 49 North Garden Road, Beijing, 100191, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guan
authors.affiliation: Department of Occupational Disease, Peking University Third Hospital, 49 North Garden Road, Beijing, 100191, China.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Occupational Disease, Peking University Third Hospital, 49 North Garden Road, Beijing, 100191, China. Electronic address: zhaojinyuan@sina.com.
authors.forename: Jinyuan
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D036145:Ginsenosides / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D042583:Lymphangiogenesis / Q000187:drug effects; D042601:Lymphatic Vessels / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D005894:Panax; D008517:Phytotherapy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D012822:Silicon Dioxide / Q000493:pharmacokinetics*; D012829:Silicosis / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C533408:Lyve1 protein, rat; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D042582:Vascular Endothelial Growth Factor C; D012822:Silicon Dioxide; D040321:Vascular Endothelial Growth Factor Receptor-3; C035054:ginsenoside Rg1
keywords: Ginsenoside Rg1; Lymphangiogenesis; Lymphatic drainage; Silicosis