title: High-quality draft genome sequence of the Thermus amyloliquefaciens type strain YIM 77409(T) with an incomplete denitrification pathway.
abstract: Thermus amyloliquefaciens type strain YIM 77409(T) is a thermophilic, Gram-negative, non-motile and rod-shaped bacterium isolated from Niujie Hot Spring in Eryuan County, Yunnan Province, southwest China. In the present study we describe the features of strain YIM 77409(T) together with its genome sequence and annotation. The genome is 2,160,855 bp long and consists of 6 scaffolds with 67.4 % average GC content. A total of 2,313 genes were predicted, comprising 2,257 protein-coding and 56 RNA genes. The genome is predicted to encode a complete glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle. Additionally, a large number of transporters and enzymes for heterotrophy highlight the broad heterotrophic lifestyle of this organism. A denitrification gene cluster included genes predicted to encode enzymes for the sequential reduction of nitrate to nitrous oxide, consistent with the incomplete denitrification phenotype of this strain.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091 People's Republic of China ; School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV USA.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV USA.
authors.forename: Senthil K
authors.initials: SK
authors.lastname: Murugapiran
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV USA.
authors.forename: Chrisabelle C
authors.initials: CC
authors.lastname: Mefferd
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275 People's Republic of China.
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091 People's Republic of China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Xian
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091 People's Republic of China.
authors.forename: Yi-Rui
authors.initials: YR
authors.lastname: Yin
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091 People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091 People's Republic of China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Yu
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Huntemann
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Alicia
authors.initials: A
authors.lastname: Clum
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Manoj
authors.initials: M
authors.lastname: Pillay
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Krishnaveni
authors.initials: K
authors.lastname: Palaniappan
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Neha
authors.initials: N
authors.lastname: Varghese
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mikhailova
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stamatis
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: T B K
authors.initials: TB
authors.lastname: Reddy
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Chew Yee
authors.initials: CY
authors.lastname: Ngan
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Chris
authors.initials: C
authors.lastname: Daum
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Shapiro
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Spunde
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Department of Energy Joint Genome Institute, Walnut Creek, CA USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091 People's Republic of China ; State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275 People's Republic of China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
authors.affiliation: School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV USA ; Nevada Institute of Personalized Medicine, University of Nevada Las Vegas, Las Vegas, NV USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Hedlund
keywords: Denitrification; Hot springs; Thermophiles; Thermus; Thermus amyloliquefaciens