title: iTRAQ-based comparative proteomic analysis of excretory-secretory proteins of schistosomula and adult worms of Schistosoma japonicum.
abstract: Schistosomiasis remains a serious public health problem with 200 million people infected and 779 million people at risk worldwide. The schistosomulum and adult worm are two stages of the complex lifecycle of Schistosoma japonicum and excretory/secretory proteins (ESPs) play a major role in host-parasite interactions. In this study, iTRAQ-coupled LC-MS/MS was used to investigate the proteome of ESPs obtained from schistosomula and adult worms of S. japonicum, and 298 differential ESPs were identified. Bioinformatics analysis of differential ESPs in the two developmental stages showed that 161 ESPs upregulated in schistosomula were associated with stress responses, carbohydrate metabolism and protein degradation, whereas ESPs upregulated in adult worms were mainly related to immunoregulation and purine metabolism. Recombinant heat shock protein 70 (HSP70) and thioredoxin peroxidase (TPx), two differential proteins identified in this study, were expressed. Further studies showed that rSjHSP70 and rSjTPx stimulated macrophages expressing high levels of the anti-inflammatory factors TGF-β, IL-10 and Arg-1, and suppressed the expression of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6 and iNOS in LPS-induced macrophages. This study provides new insights into the survival and development of schistosomes in the final host and helps identify vaccine candidates or new diagnostic reagents for schistosomiasis.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fu
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Henan Institute of Science and Technology, Xinxiang, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Han
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Hongxiao
authors.initials: H
authors.lastname: Han
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Han
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China. Electronic address: hongyang_7@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China. Electronic address: jjlin@shvri.ac.cn.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D015801:Helminth Proteins / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000378:metabolism*; D012554:Schistosomiasis japonica / Q000378:metabolism
chemical_list: D016207:Cytokines; D015801:Helminth Proteins; D020543:Proteome; D052247:Nitric Oxide Synthase Type II
keywords: Adult worms; Excretory/secretory proteins; Schistosoma japonicum; Schistosomula; iTRAQ