title: Pseudomonas aeruginosa and Its Bacterial Components Influence the Cytokine Response in Thymocytes and Splenocytes.
abstract: Infections with Pseudomonas aeruginosa may cause many different diseases. The spectrum of such infections in general includes inflammation and bacterial sepsis. Hospital-acquired pneumonia, naturally resistant to a wide range of antibiotics, is associated with a particularly high mortality rate in mechanically ventilated patients. The pathogenesis of P. aeruginosa is complex and mediated by several virulence factors, as well as cell-associated factors. We have previously demonstrated that stimulation with different bacteria triggers the cytokine response of thymocytes. In this study, we investigated the effect of P. aeruginosa and its different components on the cytokine production of immature and mature immune cells. We found that the induced cytokine pattern in the thymus and the spleen after infections with P. aeruginosa is primarily mediated by lipopolysaccharide (LPS) of the outer cell membrane, but other components of the bacterium can influence the cytokine secretion as well. Stimulation with heat-killed P. aeruginosa and LPS does not influence the amount of cytokine-producing CD4(+) T cells but instead suppresses the emergence of Th17 cells. However, stimulation with P. aeruginosa or its components triggers the interleukin-17 (IL-17) response both in thymocytes and in splenocytes. We conclude that infections with P. aeruginosa affect the cytokine secretion of immature and mature cells and that IL-17 and Th17 cells play only a minor role in the development of pathological systemic inflammatory disease conditions during P. aeruginosa infections. Therefore, other inflammatory immune responses must be responsible for septic reactions of the host.
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich Heine University, Dusseldorf, Germany andreas.weber@med.uni-duesseldorf.de.
authors.forename: Andreas
authors.identifier: http://orcid.org/0000-0002-6916-3321
authors.initials: A
authors.lastname: Weber
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich Heine University, Dusseldorf, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Zimmermann
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich Heine University, Dusseldorf, Germany.
authors.forename: Anne K
authors.initials: AK
authors.lastname: Mausberg
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich Heine University, Dusseldorf, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Dehmel
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich Heine University, Dusseldorf, Germany.
authors.forename: Bernd C
authors.initials: BC
authors.lastname: Kieseier
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich Heine University, Dusseldorf, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Hartung
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich Heine University, Dusseldorf, Germany.
authors.forename: Harald H
authors.initials: HH
authors.lastname: Hofstetter
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D016207:Cytokines