title: Investigating the Distribution of Chemical Forms of Sulfur in Prostate Cancer Tissue Using X-ray Absorption Spectroscopy.
abstract: The use of synchrotron radiation may shed more light on the study of prostate cancer, one of the leading diseases among men. In the presented study the microbeam setup at the PSI Swiss Light Source combined with fluorescence detected X-ray absorption spectroscopy (XAS) was applied to determine two-dimensional (2D) imaging of distributions of various chemical sulfur forms in prostate cancer tissue sections, since sulfur is considered important and essential in cancer progression. The research focused on prostate tissues obtained during routine prostatectomies on patients suffering from prostate cancer.Our previous studies using μ-XAS point measurements on prostate cancer cell lines showed the differences in fractions of various forms of sulfur between cancerous and non-cancerous cells. Therefore, in this experiment the chosen areas of prostate cancer tissues were scanned to get the full picture of the chemical composition of tissue, which is highly heterogeneous. The incident X-ray beams of energies tuned to spectroscopic features of the near-edge region of sulfur K-edge absorption spectra were used to provide contrast between chemical species presented in the tissue. Next, the relative content of the three main sulfur forms, found in biological systems, was calculated and the results are presented in a form of 2D color maps. These maps are correlated with the microscopic histological image of the scanned area.The main findings show that sulfur occurs in prostate tissue mainly in reduced form. The oxidized form of sulfur is present mostly in prostatic stroma, while sulfur in intermediate oxidation state is present in trace amount.
authors.affiliation: Institute of Nuclear Physics Polish Academy of Sciences, Kraków, Poland Joanna.Czapla@ifj.edu.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Czapla-Masztafiak
authors.affiliation: Chair of Pathomorphology, Jagiellonian University Medical College, Kraków, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Okoń
authors.affiliation: Gabriel Narutowicz City Specialist Hospital, Kraków, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Gałka
authors.affiliation: Swiss Light Source, Paul Scherrer Institute, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Huthwelker
authors.affiliation: Institute of Nuclear Physics Polish Academy of Sciences, Kraków, Poland.
authors.forename: Wojciech M
authors.initials: WM
authors.lastname: Kwiatek
mesh_terms: D006651:Histocytochemistry; D006801:Humans; D008297:Male; D011467:Prostate / Q000737:chemistry* / Q000601:surgery; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000601:surgery; D013455:Sulfur / Q000032:analysis*; D056928:X-Ray Absorption Spectroscopy / Q000379:methods*
chemical_list: D013455:Sulfur
keywords: Prostate cancer; Sulfur; X-ray absorption spectroscopy (XAS) imaging