title: Effect of Nanoparticle Surface on the HPLC Elution Profile of Liposomal Nanoparticles.
abstract: Nanoparticles have been used in diverse areas, and even broader applications are expected in the future. Since surface modification can influence the configuration and toxicity of nanoparticles, a rapid screening method is important to ensure nanoparticle quality.
  
  
  
  We examined the effect of the nanoparticle surface morphology on the HPLC elution profile using two types of 100-nm liposomal nanoparticles (AmBisome(Ⓡ) and DOXIL(Ⓡ)).
  
  
  
  These 100-nm-sized nanoparticles eluted before the holdup time (about 4 min), even when a column packed with particles with a relatively large pore size (30 nm) was used. The elution time of the nanoparticles increased with pegylation of the nanoparticles and protein adsorption to the nanoparticles; however, the nanoparticles still eluted before the holdup time.
  
  
  
  The results of this study indicate that HPLC is a suitable tool for rapid evaluation of the surface of liposomal nanoparticles.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Itoh
authors.affiliation: DDS Research, Global Formulation Research Japan, Pharmaceutical Science and Technology, Core Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki, 300-2635, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Yamamoto
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Santa
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Funatsu
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. masaru-kato@umin.ac.jp.
authors.forename: Masaru
authors.initials: M
authors.lastname: Kato
mesh_terms: D000327:Adsorption; D000666:Amphotericin B / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles; D020959:Polyethylene / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
chemical_list: D008055:Lipids; D011506:Proteins; C068538:liposomal amphotericin B; C506643:liposomal doxorubicin; D011092:Polyethylene Glycols; D000666:Amphotericin B; D004317:Doxorubicin; D020959:Polyethylene
keywords: HPLC; corona; nanomedicine; pegylation; surface analysis