title: Inflammatory Response Influences Treatment of Localized Aggressive Periodontitis.
abstract: We previously reported a systemic hyperinflammatory response to bacterial lipopolysaccharide (LPS) in children with localized aggressive periodontitis (LAP). Additionally, different levels of this response were observed within the LAP group. It is unknown whether this hyperinflammatory response influences the clinical response to periodontal treatment in these children. Therefore, the goal of this study was to evaluate the influence of LPS responsiveness present prior to treatment on the clinical response to treatment within the LAP cohort. Prior to treatment, peripheral blood was collected from 60 African American participants aged 5 to 21 y, free of systemic diseases, and diagnosed with LAP. Blood was stimulated with ultrapure LPS from Escherichia coli, and Luminex assays were performed to quantify 14 cytokine/chemokine levels. Principal component and cluster analyses were used to find patterns of cytokine/chemokine expression among participants and subdivide them into clusters. Three distinct clusters emerged among LAP participants: a high responder group (high level of response for INFg, IL6, and IL12p40), a mixed responder group (low for some and high for other cytokines/chemokines), and a low responder group (low overall cytokine/chemokine response). Periodontal clinical parameters were compared among these groups prior to and 3, 6, and 12 mo following treatment with mechanical debridement and systemic antibiotics. High responders presented the lowest reductions in clinical parameters after treatment, whereas the low responders presented the highest reductions. In our LAP participants, distinct patterns of LPS response were significantly predictive of changes in clinical parameters after treatment. Future studies are needed to evaluate the underlying mechanisms predicting the heterogeneity of LAP activity, severity, and response to treatment (ClinicalTrials.gov NCT01330719).
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: N
authors.initials: N
authors.lastname: Allin
authors.affiliation: Pain Research and Intervention Center of Excellence, University of Florida, Gainesville, FL, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Cruz-Almeida
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: I
authors.initials: I
authors.lastname: Velsko
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: A
authors.initials: A
authors.lastname: Vovk
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: N
authors.initials: N
authors.lastname: Hovemcamp
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: P
authors.initials: P
authors.lastname: Harrison
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: I
authors.initials: I
authors.lastname: Aukhil
authors.affiliation: Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Wallet
authors.affiliation: Department of Periodontology, University of Florida College of Dentistry, Gainesville, FL, USA Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL, USA lshaddox@dental.ufl.edu.
authors.forename: L M
authors.initials: LM
authors.lastname: Shaddox
mesh_terms: D000293:Adolescent; D000328:Adult; D001741:Black or African American; D010520:Aggressive Periodontitis / Q000276:immunology / Q000628:therapy*; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000032:analysis; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008297:Male; D057747:Periodontal Debridement; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides
keywords: chemokines; cytokines; inflammation; lipopolysaccharides; periodontal diseases; therapy