title: Influence of promoters on the production of fengycin in Bacillus spp.
abstract: Fengycin is a promising antifungal lipopeptide from Bacillus spp. synthesized by non-ribosomal peptide synthetases (NRPS). In this work, fengycin production of a spontaneous fengycin overproducing strain, Bacillus subtilis BBG21, was first compared to those of B. subtilis BBG111 (a 168 derivative), B. subtilis ATCC 21332 and Bacillus amyloliquefaciens FZB42 under two different experimental conditions. In both conditions, very high fengycin yields were obtained from strain BBG21 (480 mg/L) in comparison to its counterparts. The high efficiency of the fengycin promoter (Pfen) of BBG21 compared to the promoter of BBG111 and FZB42 was confirmed using a GFP reporter gene. Under all tested conditions, this promoter showed highest expression in comparison to the other strains. The highest fluorescence rate was obtained with mannitol as carbon source. In addition, when the Ppps promoter from B. subtilis BBG111 was replaced by promoter Pfen from BBG21, fengycin production increased about 10-fold, while no fengycin overproduction was observed when replacement was performed with Pfen from ATCC 21332. Comparative sequence analysis of these different promoters revealed one nucleotide modification in the UP element known for its importance in the regulation process. This point mutation is thus responsible for overproduction of fengycin in BBG21.
authors.affiliation: University Lille 1 Sciences and Technologies, Charles Viollette Institute, Cité Scientifique, F-59655 Villeneuve d'Ascq, France. Electronic address: yazinani77@yahoo.com.
authors.forename: Yazen
authors.initials: Y
authors.lastname: Yaseen
authors.affiliation: University Lille 1 Sciences and Technologies, Charles Viollette Institute, Cité Scientifique, F-59655 Villeneuve d'Ascq, France. Electronic address: frederique.gancel@univ-lille1.fr.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Gancel
authors.affiliation: University Lille 1 Sciences and Technologies, Charles Viollette Institute, Cité Scientifique, F-59655 Villeneuve d'Ascq, France. Electronic address: djamel.drider@univ-lille1.fr.
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
authors.affiliation: University Lille 1 Sciences and Technologies, Charles Viollette Institute, Cité Scientifique, F-59655 Villeneuve d'Ascq, France. Electronic address: Max.Bechet@univ-lille1.fr.
authors.forename: Max
authors.initials: M
authors.lastname: Béchet
authors.affiliation: University Lille 1 Sciences and Technologies, Charles Viollette Institute, Cité Scientifique, F-59655 Villeneuve d'Ascq, France. Electronic address: Philippe.jacques@polytech-lille.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Jacques
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism*; D019677:Artificial Gene Fusion; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics*; D005470:Fluorometry; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D055666:Lipopeptides / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic
chemical_list: D000935:Antifungal Agents; D004269:DNA, Bacterial; D055666:Lipopeptides; C049972:fengycin; D049452:Green Fluorescent Proteins
keywords: Bacillus subtilis; Fengycin; GFP expression; Plipastatin; Promoter role