title: Assessment of yeast Saccharomyces cerevisiae component binding to Mycobacterium avium subspecies paratuberculosis using bovine epithelial cells.
abstract: BACKGROUND
  Since yeast Saccharomyces cerevisiae and its components are being used for the prevention and treatment of enteric diseases in different species, they may also be useful for preventing Johne's disease, a chronic inflammatory bowel disease of ruminants caused by Mycobacterium avium spp. paratuberculosis (MAP). This study aimed to identify potential yeast derivatives that may be used to help prevent MAP infection. The adherence of mCherry-labeled MAP to bovine mammary epithelial cell line (MAC-T cells) and bovine primary epithelial cells (BECs) co-cultured with yeast cell wall components (CWCs) from four different yeast strains (A, B, C and D) and two forms of dead yeast from strain A was investigated.
  
  
  RESULTS
  The CWCs from all four yeast strains and the other two forms of dead yeast from strain A reduced MAP adhesion to MAC-T cells and BECs in a concentration-dependent manner after 6-h of exposure, with the dead yeast having the greatest effect.
  
  
  CONCLUSIONS
  The following in vitro binding studies suggest that dead yeast and its' CWCs may be useful for reducing risk of MAP infection.
authors.affiliation: Department of Animal Biosciences, University of Guelph, Guelph, N1G 2W1, ON, Canada. zli05@uoguelph.ca.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Animal Biosciences, University of Guelph, Guelph, N1G 2W1, ON, Canada. qyou@uoguelph.ca.
authors.forename: Qiumei
authors.initials: Q
authors.lastname: You
authors.affiliation: Lallemand Inc., Montréal, H4P 2R2, QC, Canada. faisury_o@hotmail.com.
authors.forename: Faisury
authors.initials: F
authors.lastname: Ossa
authors.affiliation: Department of Animal Biosciences, University of Guelph, Guelph, N1G 2W1, ON, Canada. philipmead05@gmail.com.
authors.forename: Philip
authors.initials: P
authors.lastname: Mead
authors.affiliation: Department of Animal Biosciences, University of Guelph, Guelph, N1G 2W1, ON, Canada. margaret@uoguelph.ca.
authors.forename: Margaret
authors.initials: M
authors.lastname: Quinton
authors.affiliation: Department of Animal Biosciences, University of Guelph, Guelph, N1G 2W1, ON, Canada. nkarrow@uoguelph.ca.
authors.forename: Niel A
authors.initials: NA
authors.lastname: Karrow
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis / Q000378:metabolism*; D010283:Paratuberculosis / Q000517:prevention & control; D012441:Saccharomyces cerevisiae / Q000378:metabolism*