title: Regulation of eIF2α expression and renal interstitial fibrosis by resveratrol in rat renal tissue after unilateral ureteral obstruction.
abstract: OBJECTIVE
  This study was performed to assess the effect of resveratrol on the expression of eukaryotic initiation factor 2α (eIF2α) and activating transcription factor 4 (ATF4) in renal tissues of rats with unilateral ureteral obstruction (UUO).
  
  
  METHODS
  Using UUO animal model, after 14 days of surgery, pathological changes were detected by HE staining, renal tubular damage index, renal interstitial collagen deposition area were evaluated by Masson staining, in situ cell apoptosis in renal tissue was analyzed by TUNEL assay, and protein expression of eIF2α and ATF4 in renal tissue was analyzed using western blot detection.
  
  
  RESULTS
  After comparison of the treatment groups with model group, we observed that the degree of renal tubular damage, relative area of renal interstitial collagen and eIF2α, ATF4 protein expression were also significantly reduced (p<0.05, p  <0.01) in the high-dose resveratrol group.
  
  
  CONCLUSIONS
  Resveratrol can reduce the level of eIF2α protein expression, which further reduces the ATF4 levels.
authors.affiliation: a College of Pharmacy , Harbin University of Commerce , Harbin , China ;
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a College of Pharmacy , Harbin University of Commerce , Harbin , China ;
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: a College of Pharmacy , Harbin University of Commerce , Harbin , China ;
authors.forename: Yajie
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: b Heilongjiang Songhuajiang Pharmaceutical Limited , Harbin , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.affiliation: a College of Pharmacy , Harbin University of Commerce , Harbin , China ;
authors.forename: Danfeng
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D015852:Eukaryotic Initiation Factor-2 / Q000096:biosynthesis* / Q000187:drug effects*; D005355:Fibrosis / Q000188:drug therapy / Q000209:etiology; D007668:Kidney / Q000473:pathology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use*; D014517:Ureteral Obstruction / Q000150:complications
chemical_list: D015852:Eukaryotic Initiation Factor-2; D013267:Stilbenes; D000077185:Resveratrol
keywords: ATF-4; eIF2α; endoplasmic reticulum stress; renal interstitial fibrosis; resveratrol