title: Proteomics in the Assessment of the Therapeutic Response of Antineoplastic Drugs: Strategies and Practical Applications.
abstract: Uncovering unknown pathological mechanisms and body response to applied medication are the driving forces toward personalized medicine. In this post-genomic era, all eyes are turned to the proteomics field, searching for answers and explanations by investigating the gene end point functional units-proteins and their proteoforms. The development of cutting-edge mass spectrometric technologies and bioinformatics tools have allowed the life-science community to discover disease-specific proteins as biomarkers, which are often concealed by high sample complexity and dynamic range of abundance. Currently, there are several proteomics-based approaches to investigate the proteome. This chapter focuses on gold standard proteomics strategies and related issues toward candidate biomarker discovery, which may have diagnostic/prognostic as well as mechanistic utility in cancer drug resistance.
authors.affiliation: Laboratory of Proteomics, Human Genetics Departament, Instituto Nacional de Saúde Dr Ricardo Jorge, Av. Padre Cruz, Lisbon, 1649-016, Portugal.|ToxOmics-Centre of Toxicogenomics and Human Health, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: Vukosava Milic
authors.initials: VM
authors.lastname: Torres
authors.affiliation: Medical Oncology Department, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia.|Medical Faculty, University of Novi Sad, Novi Sad, Serbia.
authors.forename: Lazar
authors.initials: L
authors.lastname: Popovic
authors.affiliation: Laboratory of Proteomics, Human Genetics Departament, Instituto Nacional de Saúde Dr Ricardo Jorge, Av. Padre Cruz, Lisbon, 1649-016, Portugal.|ToxOmics-Centre of Toxicogenomics and Human Health, Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: Fátima
authors.initials: F
authors.lastname: Vaz
authors.affiliation: Laboratory of Proteomics, Human Genetics Departament, Instituto Nacional de Saúde Dr Ricardo Jorge, Av. Padre Cruz, Lisbon, 1649-016, Portugal. deborah.penque@insa.min-saude.pt.|ToxOmics-Centre of Toxicogenomics and Human Health, Universidade Nova de Lisboa, Lisboa, Portugal. deborah.penque@insa.min-saude.pt.
authors.forename: Deborah
authors.initials: D
authors.lastname: Penque
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor
keywords: Cancer; Chemotherapeutic response; Discovery-based proteomics; Proteomics; Target-proteomics