title: [Assessment of the concentrations of carbonylated proteins and carbonyl reductase enzyme in mexican women with breast cancer: A pilot study].
abstract: Oxidative stress could promote the development of cancer and implicate carbonylated proteins in the carcinogenic process. The goal of this study was to assess the concentrations of carbonylated proteins and carbonyl reductase enzyme in women with breast cancer and determine whether these markers were possible indicators of tissue damage caused by the disease. A total of 120 healthy women and 123 women with a diagnosis of breast cancer were included. The concentration of carbonylated proteins in plasma and the concentration of carbonyl reductase enzyme in leukocytes were determined using the ELISA assay. There was a 3.76-fold increase in the amount of carbonylated proteins in the plasma from the patient group compared with healthy control group (5±3.27 vs. 1.33±2.31 nmol carbonyls/mg protein; p<0.05). Additionally, a 60% increase in the carbonyl reductase enzyme was observed in the patient group compared with the healthy control group (3.27±0.124 vs. 2.04±0.11 ng/mg protein; p<0.05). A positive correlation (r=0.95; p<0.001) was found between both measurements. These results suggest the presence of tissue damage produced by cancer; therefore, these parameters could be used to indicate tissue damage in cancer patients.
authors.affiliation: Laboratorio de Bioquímica y Medicina Experimental, División de Investigación Biomédica, Centro Médico Nacional 20 de Noviembre, ISSSTE, México, D.F., México.
authors.forename: José
authors.initials: J
authors.lastname: Gutiérrez-Salinas
authors.affiliation: División de Medicina Genómica, Centro Médico Nacional 20 de Noviembre, ISSSTE, México, D.F., México.
authors.forename: Liliana
authors.initials: L
authors.lastname: García-Ortiz
authors.affiliation: Laboratorio de Medicina Regenerativa e Ingeniería de Tejidos, División de Investigación Biomédica, Centro Médico Nacional 20 de Noviembre, ISSSTE, México, D.F., México.
authors.forename: Paul
authors.initials: P
authors.lastname: Mondragón-Terán
authors.affiliation: Laboratorio de Bioquímica y Medicina Experimental, División de Investigación Biomédica, Centro Médico Nacional 20 de Noviembre, ISSSTE, México, D.F., México.
authors.forename: Sergio
authors.initials: S
authors.lastname: Hernández-Rodríguez
authors.affiliation: Uromédica OSF, México, D.F., México.
authors.forename: Sotero
authors.initials: S
authors.lastname: Ramírez-García
authors.affiliation: Uromédica OSF, México, D.F., México.
authors.forename: Norma Rebeca
authors.initials: NR
authors.lastname: Núñez-Ramos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000429:Alcohol Oxidoreductases / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D001943:Breast Neoplasms / Q000097:blood*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010865:Pilot Projects; D050050:Protein Carbonylation
chemical_list: D001798:Blood Proteins; D000429:Alcohol Oxidoreductases; C531129:CBR1 protein, human