title: Isolation and molecular and phylogenetic analyses of encephalomyocarditis virus from wild boar in central China.
abstract: Encephalomyocarditis virus (EMCV) can infect many host species and cause acute myocarditis and respiratory failure in piglets, reproductive failure in pregnant sows. In this study, an EMCV strain, designated JZ1202, was isolated from semi-captive wild boars that presented with acute myocarditis and sudden death in central China. It was identified by hemagglutination inhibition (HI) assay, reverse transcription polymerase chain reaction (RT-PCR) and genome sequencing. The subsequent results showed that the virus could produce a specific cytopathic effect on BHK cells and could cause clinical symptoms and pathological changes in mice. Complete genome sequencing and multiple sequence alignment indicated that JZ1202 strain was 81.3%-99.9% identical with other isolates worldwide. Phylogenetic analysis of the whole genome, ORF, VP3/VP1 and 3D genes using neighbor-joining method revealed that JZ1202 isolate was grouped into lineage 1. The results of this study confirmed that an EMCV strain JZ 1202 isolated from wild boar in central China was fatal to mice and provided new epidemiologic data on EMCV in China.
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China. Electronic address: liuhuimin@126.com.
authors.forename: Huimin
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Xiuyuan
authors.initials: X
authors.lastname: He
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Zhou
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Zhu
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chang
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Zhian
authors.initials: Z
authors.lastname: Yin
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, China.
authors.forename: Chuanqing
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan Province, China. Electronic address: ndcht@163.com.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Chang
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000662:veterinary*; D002460:Cell Line; D004686:Encephalomyocarditis virus / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D051379:Mice; D016366:Open Reading Frames; D010802:Phylogeny; D017423:Sequence Analysis, RNA / Q000379:methods*; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
keywords: Encephalomyocarditis virus; Isolation; Phylogenetic analysis; Wild boar