title: Biodiesel production from different algal oil using immobilized pure lipase and tailor made rPichia pastoris with Cal A and Cal B genes.
abstract: In this investigation, oil extraction was performed in marine macroalgae Gracilaria edulis, Enteromorpha compressa and Ulva lactuca. The algal biomass was characterized by Scanning Electron Microscopy and Fourier Transform-Infra Red Spectroscopy. Six different pre-treatment methods were carried out to evaluate the best method for maximum oil extraction. Optimization of extraction parameters were performed and high oil yield was obtained at temperature 55°C, time 150min, particle size 0.10mm, solvent-to-solid ratio 6:1 and agitation rate 500rpm. After optimization, 9.5%, 12.18% and 10.50 (g/g) of oil extraction yield was achieved from the respective algal biomass. The rate constant for extraction was obtained as first order kinetics, by differential method. Stable intracellular Cal A and Cal B lipase producing recombinant Pichia pastoris was constructed and used as biocatalyst for biodiesel production. Comparative analysis of lipase activity and biodiesel yield was made with immobilized Candida antarctica lipase.
authors.affiliation: Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Avadi, Chennai 600062, Tamilnadu, India. Electronic address: btrbio@gmail.com.
authors.forename: B
authors.initials: B
authors.lastname: Bharathiraja
authors.affiliation: Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Avadi, Chennai 600062, Tamilnadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Ranjith Kumar
authors.affiliation: Department of Biotechnology, Anna Bio Research Foundation, Arunai Engineering College, Tiruvannamalai, Tamilnadu, India.
authors.forename: R
authors.initials: R
authors.lastname: PraveenKumar
authors.affiliation: Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Avadi, Chennai 600062, Tamilnadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Chakravarthy
authors.affiliation: Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Avadi, Chennai 600062, Tamilnadu, India.
authors.forename: D
authors.initials: D
authors.lastname: Yogendran
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, Tamilnadu, India.
authors.forename: J
authors.initials: J
authors.lastname: Jayamuthunagai
mesh_terms: D055162:Biocatalysis; D056804:Biofuels / Q000032:analysis; D001709:Biotechnology / Q000379:methods*; D003296:Cooking; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D009821:Oils / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism*; D012624:Seaweed / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D044445:Ulva / Q000378:metabolism; D014866:Waste Products
chemical_list: D056804:Biofuels; D004800:Enzymes, Immobilized; D005227:Fatty Acids; D009821:Oils; D014866:Waste Products; D008049:Lipase
keywords: Biodiesel; Lipase; Macro algae; Oil extraction; Pichia pastoris