title: Sulfuriferula thiophila sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium, and correction of the name Sulfuriferula plumbophilusWatanabe, Kojima and Fukui 2015 to Sulfuriferula plumbiphila corrig.
abstract: A novel sulfur-oxidizing bacterium designated strain mst6T was isolated from spring water of Masutomi hot spring in Japan. The cells were rod-shaped (1.2-4.0 × 0.5-0.7 μm) and Gram-stain-negative. The G+C content of genomic DNA was around 52.6 mol%. The isolate possessed summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C12 : 0 as major cellular fatty acids. Strain mst6T grew by inorganic carbon fixation and oxidation of inorganic sulfur compounds with oxygen as an electron acceptor. The isolate grew over a temperature range of 5-34 °C, a NaCl concentration range of 0-110 mM and a pH range of 4.6-8.1. Optimum growth occurred at 32 °C, in the absence of NaCl and at pH 5.9-6.2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain mst6T belongs to the family Sulfuricellaceae in the class Betaproteobacteria. The closest cultured relative was Sulfuriferula multivorans TTNT with a 16S rRNA gene sequence similarity of 97.0 %. On the basis of the data obtained in this study, strain mst6T represents a novel species of the genus Sulfuriferula, for which the name Sulfuriferula thiophila sp. nov. is proposed. The type strain is mst6T ( = NBRC 111150T = DSM 101871T). In addition, we propose correcting the name Sulfuriferula plumbophilus Watanabe, Kojima and Fukui 2015 to Sulfuriferula plumbiphila corrig. based on Rule 12c, Rule 61 and Appendix 9 of the International Code of Nomenclature of Prokaryotes.
authors.affiliation: The Institute of Low Temperature Science, Hokkaido University,Kita-19, Nishi-8, Kita-ku, Sapporo 060-0819,Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: The Institute of Low Temperature Science, Hokkaido University,Kita-19, Nishi-8, Kita-ku, Sapporo 060-0819,Japan.
authors.forename: Hisaya
authors.initials: H
authors.lastname: Kojima
authors.affiliation: The Institute of Low Temperature Science, Hokkaido University,Kita-19, Nishi-8, Kita-ku, Sapporo 060-0819,Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukui
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D045482:Hot Springs / Q000382:microbiology*; D007564:Japan; D010084:Oxidation-Reduction; D010087:Oxides / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013455:Sulfur / Q000378:metabolism*; D013457:Sulfur Compounds / Q000378:metabolism; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010087:Oxides; D012336:RNA, Ribosomal, 16S; D013457:Sulfur Compounds; C405951:sulfur monoxide; D013455:Sulfur