title: Endothelial Aquaporin-1 (AQP1) Expression Is Regulated by Transcription Factor Mef2c.
abstract: Aquaporin 1 (AQP1) is expressed in most microvasculature endothelial cells and forms water channels that play major roles in a variety of physiologic processes. This study aimed to delineate the transcriptional regulation of AQP1 by Mef2c in endothelial cells. Mef2c cooperated with Sp1 to activate human AQP1 transcription by binding to its proximal promoter in human umbilical cord vein endothelial cells (HUVEC). Over-expression of Mef2c, Sp1, or Mef2c/Sp1 increased HUVEC migration and tube-forming ability, which can be abolished AQP1 knockdown. These data indicate that AQP1 is a direct target of Mef2c in regulating angiogenesis and vasculogenesis of endothelial cells.
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Liu
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: Life Science Research Center, Beihua University, Jilin, People's Republic of China.
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Tong
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Fu-Gui
authors.initials: FG
authors.lastname: Lin
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Yan-Hang
authors.initials: YH
authors.lastname: Zhuo
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Qian
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Zeng-Bin
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Medical Examination College, Jilin Medical University, People's Republic of China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Jia
mesh_terms: D051398:Aquaporin 1 / Q000235:genetics*; D002465:Cell Movement; D005786:Gene Expression Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D064326:MEF2 Transcription Factors / Q000378:metabolism; D018919:Neovascularization, Physiologic; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000378:metabolism*
chemical_list: C496020:AQP1 protein, human; D064326:MEF2 Transcription Factors; C578040:MEF2C protein, human; D016329:Sp1 Transcription Factor; C108209:SP1 protein, human; D051398:Aquaporin 1
keywords: AQP1; Mef2c; angiogenesis; migration; vascular tube formation