title: [Detection and analysis of 26 cases of long-term non-progressors who infected HIV in Henan province].
abstract: OBJECTIVE
  To investigate the progression and drug resistance of long-term non-progressors during three follow-up in Henan province.
  
  
  METHODS
  In May 2009, 26 cases of long-term non-progressors were recruited who infected HIV more than 10 years with blood collection and supply routes, did not receive anti-retroviral therapy, CD4(+)T lymphocyte count ≥350/μl and did not show typical symptoms of AIDS from Weishi, Shangcai, and Linying of Henan Province. Continuous follow-up were conducted three times since 2009 every two years with cohort analysis, the epidemiological information of infection routes, infection time and blood were collected, and 78 parts of 10 ml EDTA anticoagulated whole blood were collected. The changes of CD4 (+) T lymphocytes, viral load, and virus gene variety were characterized from 2009 to 2014. In-house methods were used to explore primary drug resistance of long-term non-progressors. Nonparametric Kruskal-Wallis test were used to compare CD4(+) T lymphocyte count and viral load changes during different follow-up times.
  
  
  RESULTS
  The average age and infection time of 26 cases were (48.51 ± 6.75) years, (13.42 ± 4.26) years, respectively. Three follow-up times, CD4(+) T lymphocyte count P50 (P25-P75) was 573.5 (487.4-789.8), 499.8 (403.5-635.7), and 418.8 (297.6-537.8)/μl (H=63.99,P<0.001), respectively. And natural logarithm of viral load P50 (P25-P75) were 3.93 (3.43-4.55), 4.29 (3.78-4.75), 4.50 (4.01-4.81) (H=3.19,P=0.355), respectively. Subtype and phylogenetic analysis of HIV showed that prevalent cases were B subtype, accounting for 88.5% (23/26), and three cases showed restructuring changes. Two cases appeared highly resistant of 18 infected patients whose viral load >1 000 copies/ml.
  
  
  CONCLUSIONS
  The CD4(+)T lymphocyte had a declining trend, virus subtype recombinant changes in a few cases, and primary drug resistance was found of long-term non-progressors in Henan province.
authors.affiliation: Center for AIDS/STD Control and Prevention, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Xue
authors.forename: Suian
authors.initials: S
authors.lastname: Tian
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.forename: Dingyong
authors.initials: D
authors.lastname: Sun
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D002681:China; D015331:Cohort Studies; D018450:Disease Progression; D006678:HIV / Q000145:classification / Q000187:drug effects; D015658:HIV Infections / Q000453:epidemiology*; D020276:HIV Long-Term Survivors; D006801:Humans; D008875:Middle Aged; D010802:Phylogeny; D019562:Viral Load