title: SuReSim: simulating localization microscopy experiments from ground truth models.
abstract: Super-resolution fluorescence microscopy has become a widely used tool in many areas of research. However, designing and validating super-resolution experiments to address a research question in a technically feasible and scientifically rigorous manner remains a fundamental challenge. We developed SuReSim, a software tool that simulates localization data of arbitrary three-dimensional structures represented by ground truth models, allowing users to systematically explore how changing experimental parameters can affect potential imaging outcomes.
authors.affiliation: Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany.
authors.forename: Varun
authors.initials: V
authors.lastname: Venkataramani
authors.affiliation: Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Herrmannsdörfer
authors.affiliation: Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany.|Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany.
authors.forename: Mike
authors.initials: M
authors.lastname: Heilemann
authors.affiliation: Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kuner
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D012984:Software; D013572:Synaptic Vesicles / Q000648:ultrastructure*