title: Crystallization and X-ray diffraction analysis of the CH domain of the cotton kinesin GhKCH2.
abstract: GhKCH2 belongs to a group of plant-specific kinesins (KCHs) containing an actin-binding calponin homology (CH) domain in the N-terminus. Previous studies revealed that the GhKCH2 CH domain (GhKCH2-CH) had a higher affinity for F-actin (Kd = 0.42 ± 0.02 µM) than most other CH-domain-containing proteins. To understand the underlying mechanism, prokaryotically expressed GhKCH2-CH (amino acids 30-166) was purified and crystallized. Crystals were grown by the sitting-drop vapour-diffusion method using 0.1 M Tris-HCl pH 7.0, 20%(w/v) PEG 8000 as a precipitant. The crystals diffracted to a resolution of 2.5 Å and belonged to space group P21, with unit-cell parameters a = 41.57, b = 81.92, c = 83.00 Å, α = 90.00, β = 97.31, γ = 90.00°. Four molecules were found in the asymmetric unit with a Matthews coefficient of 2.22 Å(3) Da(-1), corresponding to a solvent content of 44.8%.
authors.affiliation: College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuanxilu, Haidian District, Beijing 100094, People's Republic of China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Qin
authors.affiliation: College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuanxilu, Haidian District, Beijing 100094, People's Republic of China.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuanxilu, Haidian District, Beijing 100094, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuanxilu, Haidian District, Beijing 100094, People's Republic of China.
authors.forename: Guoqin
authors.initials: G
authors.lastname: Liu
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D003368:Gossypium / Q000201:enzymology*; D016547:Kinesins / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D000072417:Protein Domains
chemical_list: D010940:Plant Proteins; D016547:Kinesins
keywords: CH domain; cotton kinesin GhKCH2; plant-specific kinesin