title: False-positive rifampin resistant results with Xpert MTB/RIF version 4 assay in clinical samples with a low bacterial load.
abstract: We report investigation of 22 TB cases with positive Xpert MTB/RIF result for resistance to Rifampin and "Very Low" MTB detection level. Twelve cases were false positive without rpoB mutations, 2 were false-positives with a silent mutation in rpoB codon T508, and only 10 were true positives.
authors.affiliation: Center for Global Health, Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, New York; Les Centres GHESKIO, Port-au-Prince, Haiti. Electronic address: oko2001@med.cornell.edu.
authors.forename: Oksana
authors.initials: O
authors.lastname: Ocheretina
authors.affiliation: Center for Global Health, Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, New York.
authors.forename: Erin
authors.initials: E
authors.lastname: Byrt
authors.affiliation: Les Centres GHESKIO, Port-au-Prince, Haiti.
authors.forename: Marie-Marcelle
authors.initials: MM
authors.lastname: Mabou
authors.affiliation: Les Centres GHESKIO, Port-au-Prince, Haiti.
authors.forename: Gertrude
authors.initials: G
authors.lastname: Royal-Mardi
authors.affiliation: Les Centres GHESKIO, Port-au-Prince, Haiti.
authors.forename: Yves-Mary
authors.initials: YM
authors.lastname: Merveille
authors.affiliation: Les Centres GHESKIO, Port-au-Prince, Haiti.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Rouzier
authors.affiliation: Center for Global Health, Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, New York.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Fitzgerald
authors.affiliation: Center for Global Health, Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, New York; Les Centres GHESKIO, Port-au-Prince, Haiti.
authors.forename: Jean W
authors.initials: JW
authors.lastname: Pape
mesh_terms: D058491:Bacterial Load; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012293:Rifampin / Q000494:pharmacology*; D013183:Sputum / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology
chemical_list: D012293:Rifampin
keywords: Developing and high-burden countries; MDR-TB; Molecular diagnostics; Tuberculosis; Xpert MTB/RIF