title: Single step purification of concanavalin A (Con A) and bio-sugar production from jack bean using glucosylated magnetic nano matrix.
abstract: Jack bean (JB, Canavalia ensiformis) is the source of bio-based products, such as proteins and bio-sugars that contribute to modern molecular biology and biomedical research. In this study, the use of jack bean was evaluated as a source for concanavalin A (Con A) and bio-sugar production. A novel method for purifying Con A from JBs was successfully developed using a glucosylated magnetic nano matrix (GMNM) as a physical support, which facilitated easy separation and purification of Con A. In addition, the enzymatic conversion rate of 2% (w/v) Con A extracted residue to bio-sugar was 98.4%. Therefore, this new approach for the production of Con A and bio-sugar is potentially useful for obtaining bio-based products from jack bean.
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Ho Myeong
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Cho
authors.affiliation: Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea; Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: baehj@chonnam.ac.kr.
authors.forename: Hyeun-Jong
authors.initials: HJ
authors.lastname: Bae
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D032449:Canavalia / Q000378:metabolism*; D002241:Carbohydrates; D003208:Concanavalin A / Q000302:isolation & purification*; D006031:Glycosylation; D009005:Monosaccharides / Q000378:metabolism; D049329:Nanostructures
chemical_list: D002241:Carbohydrates; D009005:Monosaccharides; D003208:Concanavalin A
keywords: Bio-sugar; Concanavalin A; Glucosylated magnetic nano matrix; Jack bean; Magnetic separation