title: Aliidiomarina iranensis sp. nov., a haloalkaliphilic bacterium from a coastal-marine wetland.
abstract: A novel Gram-stain-negative, straight rod-shaped, non-pigmented, slightly halophilic and alkaliphilic bacterium, designated strain GBPy7T, was isolated from a sample of the coastal-marine wetland Gomishan in Iran. Cells of strain GBPy7T were motile. Growth occurred on media with 1-15 % (w/v) NaCl (optimum 3 %), at pH 7-10 (optimum pH 8.5) and at 4-45 °C (optimum 37 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that strain GBPy7T belonged to the family Idiomarinaceae. Its closest relatives were Aliidiomarina shirensis AIST (98.1 % 16S rRNA gene sequence similarity) and other Aliidiomarina species (95.9-94.2 %), together with Idiomarina seosinensis CL-SP19T (94.3 %) and Idiomarina fontislapidosi F23T (94.3 %). The major cellular fatty acids of the isolate were iso-C15 : 0, iso-C17 : 0, iso-C17 : 1ω9c and C18 : 1ω7c and its polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown aminophospholipid. Cells of strain GBPy7T contained ubiquinone Q-8. The G+C content of the genomic DNA of this strain was 51.6 mol%. The level of DNA-DNA relatedness between strain GBPy7T and A. shirensis IBRC-M 10414T was 21 %. The physiological, biochemical, genotypic and phylogenetic differences between strain GBPy7T and other previously described taxa indicate that the strain represents a novel species of the genus Aliidiomarina within the family Idiomarinaceae, for which the name Aliidiomarina iranensis sp. nov. is proposed. The type strain is GBPy7T ( = IBRC-M 10763T = CECT 8339T).
authors.affiliation: Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran,Tehran,Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ali Amoozegar
authors.affiliation: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), Academic Center for Education, Culture and Research,Tehran,Iran.
authors.forename: Azadeh
authors.initials: A
authors.lastname: Shahinpei
authors.affiliation: Microorganisms Bank, Iranian Biological Resource Centre (IBRC), Academic Center for Education, Culture and Research,Tehran,Iran.
authors.forename: Seyed
authors.initials: S
authors.lastname: Abolhassan Shahzadeh Fazeli
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures,Inhoffenstraße 7B, 38124 Braunschweig,Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures,Inhoffenstraße 7B, 38124 Braunschweig,Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla,41012 Sevilla,Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ventosa
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007492:Iran; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013141:Spiro Compounds; D014451:Ubiquinone / Q000737:chemistry; D053833:Wetlands
chemical_list: C529377:BAS 100; D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D013141:Spiro Compounds; D014451:Ubiquinone; C483858:phosphatidylethanolamine; C030778:ubiquinone 8