title: Molecular Characterization of a Prevalent Ribocluster of Methicillin-Sensitive Staphylococcus aureus from Orthopedic Implant Infections. Correspondence with MLST CC30.
abstract: Staphylococcus aureus is the leading etiologic agent of orthopedic implant infections. Here a ribocluster of 27 S. aureus strains underwent further molecular characterization and subtyping by multilocus sequence typing (MLST) and spa-typing. This cluster had been detected by automated ribotyping (with the EcoRI restriction enzyme) of 200 S. aureus isolates from periprosthetic infections of patients who underwent revision at the Rizzoli Orthopaedic Institute. The ribocluster, consisting of agr type III strains, with a 74% co-occurrence of bone sialoprotein-binding (bbp) and collagen-binding (cna) genes, lacked mecA and IS256, and exhibited a high prevalence of the toxic shock syndrome toxin gene (tst, 85%). Strains' relatedness was analyzed by BURP and eBURST. Two predominant spa types, t012 (32%) and t021 (36%), and one predominant sequence type, ST30 (18/27, 67%) were identified: a S. aureus lineage spread worldwide belonging to MLST CC30. Two new sequence types (ST2954, ST2960) and one new spa type (t13129) were detected for the first time. Interestingly, the 27-strain cluster detected by ribotyping corresponded exactly to MLST CC30, the sole CC identified by eBURST.
authors.affiliation: Research Unit on Implant Infections, Rizzoli Orthopaedic InstituteBologna, Italy; Department of Experimental, Diagnostic, and Specialty Medicine, University of BolognaBologna, Italy.
authors.forename: Lucio
authors.initials: L
authors.lastname: Montanaro
authors.affiliation: Research Unit on Implant Infections, Rizzoli Orthopaedic InstituteBologna, Italy; Department of Experimental, Diagnostic, and Specialty Medicine, University of BolognaBologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Ravaioli
authors.affiliation: Division of Human Medicine, Austrian Agency for Health and Food Safety Vienna, Austria.
authors.forename: Werner
authors.initials: W
authors.lastname: Ruppitsch
authors.affiliation: Research Unit on Implant Infections, Rizzoli Orthopaedic Institute Bologna, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Campoccia
authors.affiliation: Unit of Biochemistry, Department of Molecular Medicine, University of Pavia Pavia, Italy.
authors.forename: Giampiero
authors.initials: G
authors.lastname: Pietrocola
authors.affiliation: Molecular Medicine Department, Center for Health Technologies (CHT), UdR INSTM, University of PaviaPavia, Italy; Department of Occupational Medicine, Toxicology and Environmental Risks, S. Maugeri FoundationIRCCS, Pavia, Italy.
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.affiliation: Unit of Biochemistry, Department of Molecular Medicine, University of Pavia Pavia, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Speziale
authors.affiliation: Division of Human Medicine, Austrian Agency for Health and Food Safety Vienna, Austria.
authors.forename: Franz
authors.initials: F
authors.lastname: Allerberger
authors.affiliation: Research Unit on Implant Infections, Rizzoli Orthopaedic InstituteBologna, Italy; Department of Experimental, Diagnostic, and Specialty Medicine, University of BolognaBologna, Italy.
authors.forename: Carla Renata
authors.initials: CR
authors.lastname: Arciola
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D021521:Ribotyping; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D018089:Superantigens / Q000235:genetics*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D004768:Enterotoxins; D046915:Penicillin-Binding Proteins; D018089:Superantigens; D037521:Virulence Factors; C048165:adhesin, Staphylococcus aureus; C405490:bone sialoprotein-binding protein, Staphylococcus aureus; C030107:enterotoxin F, Staphylococcal; C487789:mecA protein, Staphylococcus aureus
keywords: methicillin-sensitive Staphylococcus aureus; multilocus sequence typing; orthopedic implant infections; spa-typing; virulence factors