title: Effect of adenovirus-mediated RNA interference of IL-1β expression on spinal cord injury in rats.
abstract: METHODS
  We introduced an adenoviral vector expressing interleukin-1β (IL-1β) small-hairpin RNA (shRNA) into the injured spinal cords to evaluate the therapeutic potential of IL-1β downregulation in a rat model of spinal cord injury (SCI).
  
  
  OBJECTIVE
  The purpose of this study was to investigate the possible protective effects of the IL-1β downregulation on traumatic SCI in rats.
  
  
  METHODS
  Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, People's Republic of China.
  
  
  METHODS
  An adenoviral shRNA targeting IL-1β was constructed and injected at the T12 section 7 days before SCI. The rats' motor functions were evaluated by the Basso-Beattie-Bresnahan (BBB) rating scale. Immunofluorescence, enzyme-linked immunosorbent assay, flow-cytometric analysis and western blots were also performed.
  
  
  RESULTS
  Animals downregulating IL-1β had significantly better recovery of locomotor function and less neuronal loss after SCI. In addition, IL-1β downregulation significantly decreased tumor necrosis factor-alpha (TNF-α) level and Bax expression, reduced the activity of caspase-3 and increased Bcl-2 expression after SCI.
  
  
  CONCLUSIONS
  This study demonstrated that the IL-1β downregulation may have potential therapeutic benefits for both reducing secondary damages and improving the outcomes after traumatic SCI.
authors.affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: W-P
authors.initials: WP
authors.lastname: Lin
authors.affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: J-H
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Department of Neurology, Institute of Neurology, The First Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Cai
authors.affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: J-X
authors.initials: JX
authors.lastname: Shi
authors.affiliation: Department of Pharmacology and Neuroscience, Institute for Alzheimer's Disease and Aging Research, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA.
authors.forename: W-J
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Department of Pharmacology and Neuroscience, Institute for Alzheimer's Disease and Aging Research, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA.
authors.forename: G R
authors.initials: GR
authors.lastname: Choudhury
authors.affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: S-Q
authors.initials: SQ
authors.lastname: Wu
authors.affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: J-Z
authors.initials: JZ
authors.lastname: Wu
authors.affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: H-P
authors.initials: HP
authors.lastname: Wu
authors.affiliation: Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Fujian Province, People's Republic of China.
authors.forename: Q-F
authors.initials: QF
authors.lastname: Ke
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use*; D008124:Locomotion / Q000502:physiology; D008297:Male; D009460:Neurologic Examination; D034622:RNA Interference / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D020382:Interleukin-18; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; D049452:Green Fluorescent Proteins