title: Lipid Profiling of In Vitro Cell Models of Adipogenic Differentiation: Relationships With Mouse Adipose Tissues.
abstract: Our objective was to characterize lipid profiles in cell models of adipocyte differentiation in comparison to mouse adipose tissues in vivo. A novel lipid extraction strategy was combined with global lipid profiling using direct infusion and sequential precursor ion fragmentation, termed MS/MS(ALL) . Perirenal and inguinal white adipose tissue and interscapular brown adipose tissues from adult C57BL/6J mice were analyzed. 3T3-L1 preadipocytes, ear mesenchymal progenitor cells, and brown adipose-derived BAT-C1 cells were also characterized. Over 3000 unique lipid species were quantified. Principal component analysis showed that perirenal versus inguinal white adipose tissues varied in lipid composition of triacyl- and diacylglycerols, sphingomyelins, glycerophospholipids and, notably, cardiolipin CL 72:3. In contrast, hexosylceramides and sphingomyelins distinguished brown from white adipose. Adipocyte differentiation models showed broad differences in lipid composition among themselves, upon adipogenic differentiation, and with adipose tissues. Palmitoyl triacylglycerides predominate in 3T3-L1 differentiation models, whereas cardiolipin CL 72:1 and SM 45:4 were abundant in brown adipose-derived cell differentiation models, respectively. MS/MS(ALL) data suggest new lipid biomarkers for tissue-specific lipid contributions to adipogenesis, thus providing a foundation for using in vitro models of adipogenesis to reflect potential changes in adipose tissues in vivo. J. Cell. Biochem. 117: 2182-2193, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Lucy
authors.initials: L
authors.lastname: Liaw
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Igor
authors.initials: I
authors.lastname: Prudovsky
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Koza
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.
authors.forename: Rea V
authors.initials: RV
authors.lastname: Anunciado-Koza
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Siviski
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Volkhard
authors.initials: V
authors.lastname: Lindner
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Friesel
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Clifford J
authors.initials: CJ
authors.lastname: Rosen
authors.affiliation: Sciex Corporation, Framingham, Massachusetts 01701.
authors.forename: Paul R S
authors.initials: PR
authors.lastname: Baker
authors.affiliation: Sciex Corporation, Framingham, Massachusetts 01701.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Simons
authors.affiliation: Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.|The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
authors.forename: Calvin P H
authors.initials: CP
authors.lastname: Vary
mesh_terms: D041721:3T3-L1 Cells; D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000502:physiology*; D052436:Adipose Tissue, White / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D050356:Lipid Metabolism / Q000502:physiology*; D051379:Mice; D008954:Models, Biological
keywords: CARDIOLIPIN; LIPIDOMICS; MASS SPECTROMETRY; MS/MSALL; PHOSPHOLIPIDS; PREADIPOCYTE; SPHINGOLIPIDS