title: Recombinant Measles AIK-C Vaccine Strain Expressing the prM-E Antigen of Japanese Encephalitis Virus.
abstract: An inactivated Japanese encephalitis virus (JEV) vaccine, which induces neutralizing antibodies, has been used for many years in Japan. In the present study, the JEV prM-E protein gene was cloned, inserted at the P/M junction of measles AIK-C cDNA, and an infectious virus was recovered. The JEV E protein was expressed in B95a cells infected with the recombinant virus. Cotton rats were inoculated with recombinant virus. Measles PA antibodies were detected three weeks after immunization. Neutralizing antibodies against JEV developed one week after inoculation, and EIA antibodies were detected three weeks after immunization. The measles AIK-C-based recombinant virus simultaneously induced measles and JEV immune responses, and may be a candidate for infant vaccines. Therefore, the present strategy of recombinant viruses based on a measles vaccine vector would be applicable to the platform for vaccine development.
authors.affiliation: Kitasato-Daiichi Sankyo Vaccine, Division of Vaccine Production, Kitamoto City, Saitama Prefecture, 364-0026, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Higuchi
authors.affiliation: Kitasato-Daiichi Sankyo Vaccine, Division of Vaccine Development, Kitamoto City, Saitama Prefecture, 364-0026, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Toriniwa
authors.affiliation: Kitasato-Daiichi Sankyo Vaccine, Division of Vaccine Development, Kitamoto City, Saitama Prefecture, 364-0026, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Komiya
authors.affiliation: Kitasato Institute of Life Sciences, Laboratory of Viral Infection, Tokyo, 108-8641, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000276:immunology; D002522:Chlorocebus aethiops; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000517:prevention & control; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D022321:Japanese Encephalitis Vaccines / Q000276:immunology*; D008457:Measles / Q000517:prevention & control; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D051381:Rats; D014614:Vaccines, Synthetic / Q000276:immunology*; D014709:Vero Cells
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D022321:Japanese Encephalitis Vaccines; D008458:Measles Vaccine; D014614:Vaccines, Synthetic