title: Asymmetrical distribution of δ and PP cells in human pancreatic islets.
abstract: The aim of this study was to evaluate the location of PP and δ cells in relation to the vascularization within human pancreatic islets. To this end, pancreas sections were analysed by immunofluorescence using antibodies against endocrine islet and endothelial cells. Staining in different islet areas corresponding to islet cells adjacent or not to peripheral or central vascular channels was quantified by computerized morphometry. As results, α, PP and δ cells were preferentially found adjacent to vessels. In contrast to α cells, which were evenly distributed between islet periphery and intraislet vascular channels, PP and δ cells had asymmetric and opposite distributions: PP staining was higher and somatostatin staining was lower in the islet periphery than in the area around intraislet vascular channels. Additionally, frequencies of PP and δ cells were negatively correlated in the islets. No difference was observed between islets from the head and the tail of the pancreas, and from type 2 diabetic and non-diabetic donors. In conclusion, the distribution of δ cells differs from that of PP cells in human islets, suggesting that vessels at the periphery and at the centre of islets drain different hormonal cocktails.
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Barbieux
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Parnaud
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Lavallard
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Estelle
authors.initials: E
authors.lastname: Brioudes
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Meyer
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Alibashe Ahmed
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Ekaterine
authors.initials: E
authors.lastname: Berishvili
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland.
authors.forename: Thierry
authors.initials: T
authors.lastname: Berney
authors.affiliation: Department of SurgeryCell Isolation and Transplantation Center, Geneva University Hospitals and University of Geneva, Geneva, Switzerland domenico.bosco@unige.ch.
authors.forename: Domenico
authors.initials: D
authors.lastname: Bosco
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005455:Fluorescent Antibody Technique; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism*; D008875:Middle Aged; D010191:Pancreatic Polypeptide / Q000378:metabolism; D050418:Pancreatic Polypeptide-Secreting Cells / Q000166:cytology* / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism; D019864:Somatostatin-Secreting Cells / Q000166:cytology* / Q000378:metabolism*; D014018:Tissue Distribution; D055815:Young Adult
chemical_list: D013004:Somatostatin; D010191:Pancreatic Polypeptide
keywords: PP cells; architecture; pancreatic islets; δ cells