title: Comparison of isocratic retention models for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides.
abstract: In this work, we have investigated retention of maltooligosaccharides and their fluorescent derivatives in hydrophilic interaction liquid chromatography using four different stationary phases. The non-derivatized maltooligosaccharides (maltose to maltoheptaose) and their derivatives with 2-aminobenzoic acid, 2-aminobenzamide, 2-aminopyridine and 8-aminonaphthalene-1,3,6-trisulfonic acid were analyzed on silica gel, aminopropyl silica, amide (carbamoyl-bonded silica) and ZIC-HILIC zwitterionic sulfobetain bonded phase. The partitioning of the analytes between the bulk mobile phase and adsorbed water-rich layer, polar and ionic interactions of analytes with stationary phase have been evaluated and compared. The effects of the mobile phase additives (0.1% (v/v) of acetic acid and ammonium acetate in concentration range 5-30 mmol L(-1)) on retention were described. The suitability of different models for prediction of retention was tested including linear solvent strength model, quadratic model, mixed-mode model, and empirical Neue-Kuss model. The mixed-mode model was extended to the parameter describing the contribution of monomeric glucose unit to the retention of non-derivatized and derivatized maltooligosaccharides, which was used for evaluation of contribution of both, oligosaccharide backbone and end-group to retention.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice CZ-53210, Czech Republic. Electronic address: Petr.Cesla@upce.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Česla
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice CZ-53210, Czech Republic.
authors.forename: Nikola
authors.initials: N
authors.lastname: Vaňková
authors.affiliation: Institute of Analytical Chemistry of the CAS, v.v.i., Veveří 97, Brno CZ-60200 Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Křenková
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice CZ-53210, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Fischer
mesh_terms: D015057:1-Naphthylamine / Q000737:chemistry; D000327:Adsorption; D000631:Aminopyridines / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D002853:Chromatography, Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000737:chemistry
chemical_list: D000631:Aminopyridines; D009844:Oligosaccharides; C021705:maltooligosaccharides; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D012822:Silicon Dioxide; D015057:1-Naphthylamine; C000219:anthranilamide; C032439:alpha-aminopyridine
keywords: Fluorescent derivatives; Hydrophilic interaction liquid chromatography; Liquid chromatography/mass spectrometry; Oligosaccharides; Retention