title: Detection and molecular characterization of a Grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant in an autochthonous grape from Apulia (Italy).
abstract: The complete nucleotide sequence and genome organization of a new Badnavirus isolated from the autochthonous grapevine variety "Bombino nero" from Apulia (Italy) was determined. The genome of this virus consists of 7097 nt and has four open reading frames (ORFs). Analysis of putative proteins encoded by each ORF revealed greatest sequence similarity to Grapevine Roditis leaf discoloration-associated virus w4 (GRLDaV; NC_027131). In a pairwise alignment with GLRDaV w4 genome sequence, the "Bombino Nero" sequence was 109 nt longer with a major 57 nt insertion between positions 2405 and 2413. Furthermore, its putative ORF4 is located after the ORF3, while in the GLRDaV w4 sequence, the putative ORF4 completely overlapped ORF3. Nucleotide analysis classifies this new Badnavirus as a GLRDaV strain, which was named GRLDaV-BN. Multi-year field observations showed that the GLRDaV-BN-infected vine was symptomless.
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, Via Amendola 122/D, 70126, Bari, Italy. michela.chiumenti@ipsp.cnr.it.
authors.forename: Michela
authors.initials: M
authors.lastname: Chiumenti
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, Via Amendola 122/D, 70126, Bari, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Giampetruzzi
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, Via Amendola 122/D, 70126, Bari, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Morelli
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti (DiSSPA), Università degli Studi di Bari Aldo Moro, 70126, Bari, Italy.|Centro di Ricerca, Sperimentazione e Formazione in Agricoltura (CRSFA) "Basile Caramia", Locorotondo, 70010, Bari, Italy.
authors.forename: Vito Nicola
authors.initials: VN
authors.lastname: Savino
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti (DiSSPA), Università degli Studi di Bari Aldo Moro, 70126, Bari, Italy.
authors.forename: Giovanni Paolo
authors.initials: GP
authors.lastname: Martelli
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, Via Amendola 122/D, 70126, Bari, Italy.|Centro di Ricerca, Sperimentazione e Formazione in Agricoltura (CRSFA) "Basile Caramia", Locorotondo, 70010, Bari, Italy.
authors.forename: Pierfederico
authors.initials: P
authors.lastname: La Notte
authors.affiliation: Centro di Ricerca, Sperimentazione e Formazione in Agricoltura (CRSFA) "Basile Caramia", Locorotondo, 70010, Bari, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Palmisano
authors.affiliation: CNR-Istituto per la Protezione Sostenibile delle Piante (IPSP), UOS Bari, Via Amendola 122/D, 70126, Bari, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Saldarelli
mesh_terms: D000595:Amino Acid Sequence; D019195:Badnavirus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D007558:Italy; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Autochthonous cultivars; Badnavirus; Bombino nero; Grapevine Roditis leaf discoloration-associated virus; NGS