title: The screening and evaluation of herbs and identification of herbal combinations with anti-viral effects on Newcastle disease virus.
abstract: A total of 25 "heat-clearing and detoxifying" herbs used in Chinese medicine were investigated for their cytopathic effects on the growth of Newcastle Disease virus (NDV) in a chicken fibroblast cell line. The 5 herbs with the highest virus inhibitory effects were Herba agastaches, Flos chrysanthemi indici, Rhizoma anemarrhenae, Astragalus root and Baikal skullcap root and these were used in herbal formulations. Anti-NDV activities of 4 formulations were tested on the growth of NDV in the DF-1 fibroblast cell line. Formulation II, containing Baikal skullcap root, Astragalus root, Anemarrhena rhizome (1:1:2) and formulation IV containing Anemarrhena rhizome, Astragalus root and Flos chrysanthemi indici (1:1:1), which had strong anti-NDV activity in vitro, were used to determine the in vivo inhibitory effects of NDV-infection in chickens. After treatment with the two formulations serum IgY titres against NDV were improved, and morbidity was reduced in the NDV-infected chickens. The results suggest that the components in formulations II and IV acted synergistically to improve resistance to Newcastle disease and provide a basis for the developing an anti-NDV herbal medicine.
authors.affiliation: a College of Veterinary Medicine , Northwest A&F University , Yangling , China.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: b Animal Facilities, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences , Lanzhou , China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.affiliation: c College of Chemistry and Biological Sciences , Yili Normal University , Yining , China.
authors.forename: K
authors.initials: K
authors.lastname: Brad
authors.affiliation: a College of Veterinary Medicine , Northwest A&F University , Yangling , China.
authors.forename: W
authors.initials: W
authors.lastname: Xie
authors.affiliation: a College of Veterinary Medicine , Northwest A&F University , Yangling , China.
authors.forename: X-Y
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D008516:Medicine, Chinese Traditional; D009521:Newcastle Disease / Q000188:drug therapy*; D009522:Newcastle disease virus / Q000187:drug effects*; D008517:Phytotherapy / Q000662:veterinary*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011201:Poultry Diseases / Q000188:drug therapy*
chemical_list: D010936:Plant Extracts