title: Improving understanding of chromatin regulatory proteins and potential implications for drug discovery.
abstract: Many epigenetic-based therapeutics, including drugs such as histone deacetylase inhibitors, are now used in the clinic or are undergoing advanced clinical trials. The study of chromatin-modifying proteins has benefited from the rapid advances in high-throughput sequencing methods, the organized efforts of major consortiums and by individual groups to profile human epigenomes in diverse tissues and cell types. However, while such initiatives have carefully characterized healthy human tissue, disease epigenomes and drug-epigenome interactions remain very poorly understood. Reviewed here is how high-throughput sequencing improves our understanding of chromatin regulator proteins and the potential implications for the study of human disease and drug development and discovery.
authors.affiliation: a Epigenetics in Human Health and Disease Laboratory , Baker IDI Heart and Diabetes Institute , Melbourne , Victoria , Australia.
authors.forename: Haloom
authors.initials: H
authors.lastname: Rafehi
authors.affiliation: a Epigenetics in Human Health and Disease Laboratory , Baker IDI Heart and Diabetes Institute , Melbourne , Victoria , Australia.|b Department of Pathology , The University of Melbourne , Parkville , Victoria , Australia.
authors.forename: Abdul Waheed
authors.initials: AW
authors.lastname: Khan
authors.affiliation: a Epigenetics in Human Health and Disease Laboratory , Baker IDI Heart and Diabetes Institute , Melbourne , Victoria , Australia.|b Department of Pathology , The University of Melbourne , Parkville , Victoria , Australia.|c Faculty of Medicine , Monash University , Melbourne , Victoria , Australia.
authors.forename: Assam
authors.initials: A
authors.lastname: El-Osta
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly; D055808:Drug Discovery / Q000379:methods*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D011496:Protein Methyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; D011496:Protein Methyltransferases; D006655:Histone Deacetylases
keywords: Epigenetics; HDAC inhibitors; chromatin modifications; high-throughput sequencing; histone acetylation