title: Metabolomic Assessment of Key Maize Resources: GC-MS and NMR Profiling of Grain from B73 Hybrids of the Nested Association Mapping (NAM) Founders and of Geographically Diverse Landraces.
abstract: The present study expands metabolomic assessments of maize beyond commercial lines to include two sets of hybrids used extensively in the scientific community. One set included hybrids derived from the nested association mapping (NAM) founder lines, a collection of 25 inbreds selected on the basis of genetic diversity and used to investigate the genetic basis of complex plant traits. A second set included 24 hybrids derived from a collection of landraces representative of native diversity from North and South America that may serve as a source of new alleles for improving modern maize hybrids. Metabolomic analysis of grain harvested from these hybrids utilized gas chromatography-time-of-flight mass spectrometry (GC-TOF-MS) and (1)H nuclear magnetic resonance spectroscopy ((1)H NMR) techniques. Results highlighted extensive metabolomic variation in grain from both hybrid sets, but also demonstrated that, within each hybrid set, subpopulations could be differentiated in a pattern consistent with the known genetic and compositional variation of these lines. Correlation analysis did not indicate a strong association of the metabolomic data with grain nutrient composition, although some metabolites did show moderately strong correlations with agronomic features such as plant and ear height. Overall, this study provides insights into the extensive metabolomic diversity associated with conventional maize germplasm.
authors.affiliation: Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
authors.forename: Tyamagondlu V
authors.initials: TV
authors.lastname: Venkatesh
authors.affiliation: Genome Center - Metabolomics, University of California at Davis , Davis, California 95616, United States.
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Chassy
authors.affiliation: Genome Center - Metabolomics, University of California at Davis , Davis, California 95616, United States.|Biochemistry Department, King Abudalaziz University , Jeddah, Saudi-Arabia.
authors.forename: Oliver
authors.initials: O
authors.lastname: Fiehn
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture , Columbia, Missouri 65211, United States.|Division of Plant Sciences, University of Missouri , Columbia, Missouri 65211, United States.
authors.forename: Sherry
authors.initials: S
authors.lastname: Flint-Garcia
authors.affiliation: Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Skogerson
authors.affiliation: Monsanto Company , 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, United States.
authors.forename: George G
authors.initials: GG
authors.lastname: Harrigan
mesh_terms: D000483:Alleles; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D008279:Magnetic Resonance Imaging / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D012639:Seeds / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism
keywords: GC-TOF-MS; NMR; landraces; maize hybrids; metabolomics; nested association mapping (NAM) founders