title: Evidence for the recruitment of autophagic vesicles in human brain after stroke.
abstract: Autophagy is a homeostatic process for recycling proteins and organelles that is increasingly being proposed as a therapeutic target for acute and chronic neurodegenerative diseases, including stroke. Confirmation that autophagy is present in the human brain after stroke is imperative before prospective therapies can begin the translational process into clinical trials. Our current study using human post-mortem tissue observed an increase in staining in microtubule-associated protein 1 light chain 3 (LC3), sequestosome 1 (SQSTM1; also known as p62) and the increased appearance of autophagic vesicles after stroke. These data confirm that alterations in autophagy take place in the human brain after stroke and suggest that targeting autophagic processes after stroke may have clinical significance.
authors.affiliation: Department of Pharmacology & Therapeutics, University of Melbourne, Parkville, Australia.
authors.forename: Tony
authors.initials: T
authors.lastname: Frugier
authors.affiliation: Department of Pharmacology & Therapeutics, University of Melbourne, Parkville, Australia.
authors.forename: Juliet M
authors.initials: JM
authors.lastname: Taylor
authors.affiliation: Department of Anatomical Pathology, The Alfred Hospital, Melbourne, Australia.
authors.forename: Catriona
authors.initials: C
authors.lastname: McLean
authors.affiliation: Department of Anatomy and Neuroscience, University of Melbourne, Parkville, Australia.
authors.forename: Nicole
authors.initials: N
authors.lastname: Bye
authors.affiliation: Department of Pharmacology & Therapeutics, University of Melbourne, Parkville, Australia; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Australia.
authors.forename: Philip M
authors.initials: PM
authors.lastname: Beart
authors.affiliation: Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Victoria, Australia.
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Devenish
authors.affiliation: Department of Pharmacology & Therapeutics, University of Melbourne, Parkville, Australia. Electronic address: pcrack@unimelb.edu.au.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Crack
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001343:Autophagy / Q000502:physiology*; D000071186:Beclin-1 / Q000032:analysis / Q000096:biosynthesis*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000096:biosynthesis*; D000071456:Sequestosome-1 Protein / Q000032:analysis / Q000096:biosynthesis*; D020521:Stroke / Q000378:metabolism* / Q000473:pathology
chemical_list: C491997:BECN1 protein, human; D000071186:Beclin-1; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein
keywords: Autophagy; Brain; Human; LC3; P62; Stroke