title: COMPARISON OF PERMANENT STAINING METHODS FOR THE LABORATORY DIAGNOSIS OF TRICHOMONIASIS.
abstract: Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. The diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. The aim of this study was to compare the performance of different fixing and staining techniques used in the detection of T. vaginalis in urine. The smears were fixed and submitted to different methods of permanent staining and then, the morphological aspects of the parasites were analyzed and compared. The Papanicolaou staining with ethanol as the fixative solution showed to be the best method of permanent staining. Our data suggest that staining techniques in association with wet mount examination of fresh specimens contribute to increase the sensitivity in the diagnosis of trichomoniasis.
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Camila Braz
authors.initials: CB
authors.lastname: Menezes
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Mariana dos Santos
authors.initials: Mdos S
authors.lastname: Mello
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Tiana
authors.initials: T
authors.lastname: Tasca
mesh_terms: D003470:Culture Media; D005260:Female; D006801:Humans; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*; D014245:Trichomonas Infections / Q000175:diagnosis*; D014246:Trichomonas vaginalis / Q000302:isolation & purification* / Q000382:microbiology; D014626:Vaginal Smears
chemical_list: D003470:Culture Media