title: [Preservation of live eggs of Schistosoma japonicum].
abstract: OBJECTIVE
  To observe the preservation time and activity of miracidium hatching from schistosome eggs preserved in different solutions, so as to obtain the optimal preservation conditions and then provide quality control products for field application.
  
  
  METHODS
  The rectum stool of rabbits infected with Schistosoma japonicum was collected and the coarse fecal residue was removed with a series of sample sieves of 80, 100, 160 and 200 meshes respectively, and then the schistosome eggs were concentrated with the sample sieve of 260 meshes. The concentrated eggs were preserved in 0.9% sodium chloride solution, 1.2% sodium chloride solution, phosphate buffered saline solution (PBS, PH 7.2), 1.0% sucrose solution, and Mili-Q water, respectively, and then were conserved in a 4 °C refrigerator and 15 °C constant temperature incubator, respectively. The preserved eggs were hatched in different time (7-day interval) , the vitality and quantity of the miracidia were observed, and the hatching rates were calculated.
  
  
  RESULTS
  Under the condition of 4 °C, the hatching rates of eggs dropped to 0 in 1.0% sucrose solution and 1.2% sodium chloride solution at the 49th and 126th day, respectively, and the hatching rates of eggs in the 0.9% sodium chloride solution and PBS solution dropped to 10% at the 112th day, and the activity of miracidium was weakened since 140th. In the Mili-Q water, the hatching rate dropped less than 10% at the 196th day and the activity of miracidia was weakened since the 280th day. Under the condition of 15 °C, the hatching rate of eggs in different solutions gradually dropped to 0 from the 49th day to 105th day.
  
  
  CONCLUSIONS
  The eggs preserved in Mili-Q water at the temperature of 4 °C can be used as the positive reference for hatching tests within 196 days.
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Lan
authors.forename: Shu-ying
authors.initials: SY
authors.lastname: Xie
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei-sheng
authors.initials: WS
authors.lastname: Jiang
authors.forename: Ren-mei
authors.initials: RM
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zeng
mesh_terms: D000818:Animals; D003080:Cold Temperature; D005243:Feces / Q000469:parasitology; D005260:Female; D010063:Ovum / Q000187:drug effects / Q000502:physiology*; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology; D011817:Rabbits; D012549:Schistosoma japonicum / Q000302:isolation & purification / Q000502:physiology*; D012554:Schistosomiasis japonica / Q000469:parasitology*; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D011310:Preservatives, Pharmaceutical; D012965:Sodium Chloride; D013395:Sucrose