title: [Recombinase Polymerase Amplification and its Applications in Parasite Detection].
abstract: Recombinase polymerase amplification (RPA) is a recently -developed isothermal nucleic-acid-amplification technology that is based on the nucleic acid replication mechanism in T4 bacteriophage. With this technique, nucleic-acid templates can be amplified to measurable levels within 20 min at 37-42 °C. The. RPA process has high sensitivity and specificity, and is simple to operate, thus nucleic acids can be detected rapidly in non-laboratory conditions. Since its development in 2006, the RPA technique has been applied in agriculture, food safety, medicine, transgene detection, etc. In this review, we will give an overview on the research progress of RPA and its application in parasite detection.
authors.forename: Wen-bin
authors.initials: WB
authors.lastname: ZHENG
authors.forename: Yao-dong
authors.initials: YD
authors.lastname: WU
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: MA
authors.forename: Xing-quan
authors.initials: XQ
authors.lastname: ZHU
authors.forename: Dong-hui
authors.initials: DH
authors.lastname: ZHOU
mesh_terms: D000818:Animals; D004259:DNA-Directed DNA Polymerase; D021141:Nucleic Acid Amplification Techniques; D010271:Parasites / Q000302:isolation & purification*; D045522:Recombinases
chemical_list: D045522:Recombinases; D004259:DNA-Directed DNA Polymerase