title: Rational design of an improved tissue-engineered vascular graft: determining the optimal cell dose and incubation time.
abstract: OBJECTIVE
  We investigated the effect of cell seeding dose and incubation time on tissue-engineered vascular graft (TEVG) patency.
  
  
  METHODS
  Various doses of bone marrow-derived mononuclear cells (BM-MNCs) were seeded onto TEVGs, incubated for 0 or 12 h, and implanted in C57BL/6 mice. Different doses of human BM-MNCs were seeded onto TEVGs and measured for cell attachment.
  
  
  RESULTS
  The incubation time showed no significant effect on TEVG patency. However, TEVG patency was significantly increased in a dose-dependent manner. In the human graft, more bone marrow used for seeding resulted in increased cell attachment in a dose-dependent manner.
  
  
  CONCLUSIONS
  Increasing the BM-MNC dose and reducing incubation time is a viable strategy for improving the performance and utility of the graft.
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Yong-Ung
authors.initials: YU
authors.lastname: Lee
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Mahler
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Cameron A
authors.initials: CA
authors.lastname: Best
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Tara
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Tadahisa
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Avione Y
authors.initials: AY
authors.lastname: Lee
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Tai
authors.initials: T
authors.lastname: Yi
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Narutoshi
authors.initials: N
authors.lastname: Hibino
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Shinoka
authors.affiliation: Tissue Engineering Program, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive - WB4151, Columbus, OH 43205-2664, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Breuer
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D051379:Mice; D023822:Tissue Engineering / Q000379:methods*
keywords: BM-MNC; C57BL/6 mice; Fontan operation; TEVG; bone marrow mononuclear cells; congenital heart defect; regenerative medicine; tissue engineering