title: Bioactive PLGA-curcumin microparticle-embedded chitosan scaffold: in vitro and in vivo evaluation.
abstract: Wound healing is a complex process affected by several factors. In the present work, novel biocompatible PLGA-curcumin microparticle-embedded chitosan scaffold was fabricated for wound-healing application. Process variables involved in the preparation of microparticles were optimized using design of experiment. Scanning electron microscopy (SEM) confirmed the porous nature of scaffold with embedded microparticles. A maximum release of 14% of the encapsulated curcumin was observed at 12th hour. Modified tube dilution method showed that scaffold significantly (p < 0.05) reduced multiplication of Staphylococcus aureus. More than 50% of the excised wound in rats healed in 4 days with an epithilization period of 18 days.
authors.affiliation: a Department of Pharmaceutics , Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , Karnataka , India.
authors.forename: Muthukumar
authors.initials: M
authors.lastname: Amirthalingam
authors.affiliation: b Department of Pharmaceutical Biotechnology , Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , Karnataka , India.
authors.forename: Narayanan
authors.initials: N
authors.lastname: Kasinathan
authors.affiliation: c Department of Pharmacology , Melaka Manipal Medical College and Center for Integrative Medicine & Research, Manipal University , Manipal , Karnataka , India.
authors.forename: Arul
authors.initials: A
authors.lastname: Amuthan
authors.affiliation: a Department of Pharmaceutics , Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , Karnataka , India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Mutalik
authors.affiliation: a Department of Pharmaceutics , Manipal College of Pharmaceutical Sciences, Manipal University , Manipal , Karnataka , India.
authors.forename: M
authors.initials: M
authors.lastname: Sreenivasa Reddy
authors.affiliation: d Directorate of Research , Manipal University , Manipal , Karnataka , India.
authors.forename: Udupa
authors.initials: U
authors.lastname: Nayanabhirama
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013211:Staphylococcus aureus / Q000254:growth & development; D054457:Tissue Scaffolds / Q000737:chemistry*; D014709:Vero Cells; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan; D003474:Curcumin
keywords: Chitosan scaffold; curcumin microparticles; design of experiments; tissue regeneration; wound healing