title: The ESCRT-II proteins are involved in shaping the sarcoplasmic reticulum in C. elegans.
abstract: The sarcoplasmic reticulum is a network of tubules and cisternae localized in close association with the contractile apparatus, and regulates Ca(2+)dynamics within striated muscle cell. The sarcoplasmic reticulum maintains its shape and organization despite repeated muscle cell contractions, through mechanisms which are still under investigation. The ESCRT complexes are essential to organize membrane subdomains and modify membrane topology in multiple cellular processes. Here, we report for the first time that ESCRT-II proteins play a role in the maintenance of sarcoplasmic reticulum integrity inC. elegans ESCRT-II proteins colocalize with the sarcoplasmic reticulum marker ryanodine receptor UNC-68. The localization at the sarcoplasmic reticulum of ESCRT-II and UNC-68 are mutually dependent. Furthermore, the characterization of ESCRT-II mutants revealed a fragmentation of the sarcoplasmic reticulum network, associated with an alteration of Ca(2+)dynamics. Our data provide evidence that ESCRT-II proteins are involved in sarcoplasmic reticulum shaping.
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette cedex 91198, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Lefebvre
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette cedex 91198, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Largeau
authors.affiliation: Brigham and Women's Hospital, 1 Jimmy Fund Way, Boston, MA 02115, USA.
authors.forename: Xavier
authors.initials: X
authors.lastname: Michelet
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette cedex 91198, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Fourrage
authors.affiliation: Faculté de médecine Paris Descartes, Inserm U1001 - 24, rue du Faubourg St-Jacques, Paris 75014, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Maniere
authors.affiliation: Faculté de médecine Paris Descartes, Inserm U1001 - 24, rue du Faubourg St-Jacques, Paris 75014, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Matic
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette cedex 91198, France renaud.legouis@i2bc.paris-saclay.fr emmanuel.culetto@i2bc.paris-saclay.fr.
authors.forename: Renaud
authors.initials: R
authors.lastname: Legouis
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette cedex 91198, France renaud.legouis@i2bc.paris-saclay.fr emmanuel.culetto@i2bc.paris-saclay.fr.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Culetto
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D056827:Endosomal Sorting Complexes Required for Transport / Q000378:metabolism*; D032342:Muscle Cells / Q000378:metabolism*; D009119:Muscle Contraction / Q000502:physiology*; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D019837:Ryanodine Receptor Calcium Release Channel; C438601:Unc-68 protein, C elegans; D002118:Calcium
keywords: Caenorhabditis elegans; ESCRT-II; Organelle shape; Sarcoplasmic reticulum