title: KLF7 Regulates Satellite Cell Quiescence in Response to Extracellular Signaling.
abstract: Retaining muscle stem satellite cell (SC) quiescence is important for the maintenance of stem cell population and tissue regeneration. Accumulating evidence supports the model where key extracellular signals play crucial roles in maintaining SC quiescence or activation, however, the intracellular mechanisms that mediate niche signals to control SC behavior are not fully understood. Here, we reported that KLF7 functioned as a key mediator involved in low-level TGF-β signaling and canonical Notch signaling-induced SC quiescence and myoblast arrest. The data obtained showed that KLF7 was upregulated in quiescent SCs and nonproliferating myoblasts. Silence of KLF7 promoted SCs activation and myoblasts proliferation, but overexpression of KLF7 induced myogenic cell arrest. Notably, the expression of KLF7 was regulated by TGF-β and Notch3 signaling. Knockdown of KLF7 diminished low-level TGF-β and canonical Notch signaling-induced SC quiescence. Investigation into the mechanism revealed that KLF7 regulation of SC function was dependent on p21 and acetylation of Lys227 and/or 231 in the DNA binding domain of KLF7. Our study provides new insights into the regulatory network of muscle stem cell quiescence. Stem Cells 2016;34:1310-1320.
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.|College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, People's Republic of China.
authors.forename: Qingwu W
authors.initials: QW
authors.lastname: Shen
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Qilu University of Technology, Jinan, Shandong, People's Republic of China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Fu
authors.initials: F
authors.lastname: Feng
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Animal Science, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D024510:Muscle Development; D051880:Receptors, Notch / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction; D015533:Transcriptional Activation / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; C426008:Klf7 protein, mouse; D051741:Kruppel-Like Transcription Factors; D051880:Receptors, Notch; D016212:Transforming Growth Factor beta
keywords: KLF7; Notch; Quiescence; Satellite cells; TGF-β