title: Plasma endotoxin activity in kangaroos with oral necrobacillosis (lumpy jaw disease) using an automated handheld testing system.
abstract: The aim of the present study was to evaluate the reliability and effectiveness of directly determining endotoxin activity in plasma samples from kangaroos with lumpy jaw disease (LJD, n=15) and healthy controls (n=12). Prior to the present study, the ability of the commercially available automated handheld portable test system (PTS(TM)) to detect endotoxin activity in kangaroo plasma was compared with that of the traditional LAL-kinetic turbidimetric (KT) assay. Plasma samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS(TM) was not significantly different from that of the traditional LAL-based assay. The data obtained using PTS(TM) correlated with those using KT (r(2)=0.963, P<0.001). These findings indicated that the PTS(TM) is applicable as a simplified system to assess endotoxin activity in macropods. In the present study, we demonstrated the diagnostic value of plasma endotoxin activity in kangaroos with systemic inflammation caused by oral necrobacillosis and identified plasma endotoxin activity as a sensitive marker of systemic inflammation in kangaroos with LJD. Based on ROC curves, we proposed a diagnostic cut-off point for endotoxin activity of >0.22 EU/ml for the identification of LJD. Our results indicate that the assessment of plasma endotoxin activity is a promising diagnostic tool for determining the outcome of LJD in captive macropods.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Sotohira
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Suzuki
authors.forename: Haruka
authors.initials: H
authors.lastname: Sasaki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sano
authors.forename: Masakazu
authors.initials: M
authors.lastname: Tsuchiya
authors.forename: Yohko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Toshio
authors.initials: T
authors.lastname: Shimamori
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsukano
authors.forename: Ayano
authors.initials: A
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokota
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Asakawa
mesh_terms: D000818:Animals; D004731:Endotoxins / Q000097:blood*; D005674:Fusobacterium Infections / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D007571:Jaw Diseases / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D007614:Macropodidae / Q000097:blood / Q000382:microbiology*; D009059:Mouth Diseases / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods / Q000662:veterinary
chemical_list: D004731:Endotoxins