title: Data in support of Gallium (Ga(3+)) antibacterial activities to counteract E. coli and S. epidermidis biofilm formation onto pro-osteointegrative titanium surfaces.
abstract: This paper contains original data supporting the antibacterial activities of Gallium (Ga(3+))-doped pro-osteointegrative titanium alloys, obtained via Anodic Spark Deposition (ASD), as described in "The effect of silver or gallium doped titanium against the multidrug resistant Acinetobacter baumannii" (Cochis et al. 2016) [1]. In this article we included an indirect cytocompatibility evaluation towards Saos2 human osteoblasts and extended the microbial evaluation of the Ga(3+) enriched titanium surfaces against the biofilm former Escherichia coli and Staphylococcus epidermidis strains. Cell viability was assayed by the Alamar Blue test, while bacterial viability was evaluated by the metabolic colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Finally biofilm morphology was analyzed by Scanning Electron Microscopy (SEM). Data regarding Ga(3+) activity were compared to Silver.
authors.affiliation: Department of Health Sciences, Medical School, University of Piemonte Orientale (UPO), Via Solaroli 17, 28100 Novara, Italy; Department of Biomedical, Surgical and Dental Sciences, Università di Milano/ University of Milan, via Beldiletto 1, 20142 Milan, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cochis
authors.affiliation: Department of Health Sciences, Medical School, University of Piemonte Orientale (UPO), Via Solaroli 17, 28100 Novara, Italy; Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM), Via Giusti 9, Local Unit Piemonte Orientale, 50121 Firenze, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Azzimonti
authors.affiliation: Department of Health Sciences, Medical School, University of Piemonte Orientale (UPO), Via Solaroli 17, 28100 Novara, Italy; Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM), Via Giusti 9, Local Unit Piemonte Orientale, 50121 Firenze, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Sorrentino
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering 'G.Natta', Politecnico di Milano, Via Mancinelli 7, 20131 Milano, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Della Valle
authors.affiliation: Department of Chemistry, University of Bari "Aldo Moro", Via E. Orabona 4, 70126 Bari, Italy.
authors.forename: E
authors.initials: E
authors.lastname: De Giglio
authors.affiliation: Molecular Medicine Department, Center for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli 3/b, 27100 Pavia, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Bloise
authors.affiliation: Molecular Medicine Department, Center for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli 3/b, 27100 Pavia, Italy; Department of Occupational Medicine, Toxicology and Environmental Risks, Laboratory of Nanotechnology, Salvatore Maugeri Foundation, IRCCS, Via Boezio 28, 27100 Pavia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Visai
authors.affiliation: Department of Chemistry - Physical-Chemistry Section, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bruni
authors.affiliation: Jaber Innovation s.r.l., Via Calcutta 8, 00100 Roma, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Cometa
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering 'G.Natta', Politecnico di Milano, Via Mancinelli 7, 20131 Milano, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Pezzoli
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering 'G.Natta', Politecnico di Milano, Via Mancinelli 7, 20131 Milano, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Candiani
authors.affiliation: Department of Health Sciences, Medical School, University of Piemonte Orientale (UPO), Via Solaroli 17, 28100 Novara, Italy; Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM), Via Giusti 9, Local Unit Piemonte Orientale, 50121 Firenze, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Rimondini
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering 'G.Natta', Politecnico di Milano, Via Mancinelli 7, 20131 Milano, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Chiesa
keywords: ASD, Anodic Spark Deposition; AgCis, Antibacterial agent (AgNO3) with chelating agent (L-Cysteine); AgNPs, Silver NanoParticles; Anodic Spark Deposition; Biofilm; E. coli; GaCis, Antibacterial agent (Ga (NO3)3) with chelating agent (L-Cysteine); GaOss, Antibacterial agent (Ga (NO3)3) with chelating agent (oxalic acid); Gallium; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; S. epidermidis; SEM, Scanning Electron Microscopy; Silver; Titanium