title: Occidiofungin is an important component responsible for the antifungal activity of Burkholderia pyrrocinia strain Lyc2.
abstract: OBJECTIVE
  To identify the taxonomy of tobacco rhizosphere-isolated strain Lyc2 and investigate the mechanisms of the antifungal activities, focusing on antimicrobials gene clusters identification and function analysis.
  
  
  RESULTS
  Multilocus sequence typing and 16S rRNA analyses indicated that strain Lyc2 belongs to Burkholderia pyrrocinia. Bioassay results indicated strain Lyc2 showed significant antifungal activities against a broad range of plant and animal fungal pathogens and control efficacy on seedling damping off disease of cotton. A 55·2-kb gene cluster which was homologous to ocf gene clusters in Burkholderia contaminans MS14 was confirmed to be responsible for antifungal activities by random mutagenesis; HPLC was used to verify the production of antifungal compounds. Multiple antibiotic and secondary metabolized biosynthesis gene clusters predicated by antiSMASH revealed the broad spectrum of antimicrobials activities of the strain.
  
  
  CONCLUSIONS
  Our results revealed the mechanisms of antifungal activities of strain Lyc2 and expand our knowledge about production of occidiofungin in the bacteria Burkholderia.
  
  
  CONCLUSIONS
  Understanding the mechanisms of antifungal activities of strain Lyc2 has contributed to discovery of new antibiotics and expand our knowledge of production of occidiofungin in the bacteria Burkholderia.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.|Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn, Shandong Agricultural University, Tai'an, Shandong, China.|Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: A X
authors.initials: AX
authors.lastname: Liu
authors.affiliation: Department of Biology, Texas A&M University, College Station, TX, USA.
authors.forename: A
authors.initials: A
authors.lastname: Guerrero
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.|Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Yu
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.
authors.forename: P
authors.initials: P
authors.lastname: Deng
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Baird
authors.affiliation: Department of Biology, Texas A&M University, College Station, TX, USA.
authors.forename: L
authors.initials: L
authors.lastname: Smith
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.|Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Lu
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D019117:Burkholderia / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005658:Fungi / Q000187:drug effects; D006020:Glycopeptides / Q000378:metabolism / Q000494:pharmacology*; D005810:Multigene Family; D010456:Peptides, Cyclic / Q000378:metabolism / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D000935:Antifungal Agents; D006020:Glycopeptides; D010456:Peptides, Cyclic; D012336:RNA, Ribosomal, 16S; C576718:occidiofungin
keywords: Burkholderia pyrrocinia; antifungal activity; multilocus sequence typing; occidiofungin; strain Lyc2