title: Improved secondary caries resistance via augmented pressure displacement of antibacterial adhesive.
abstract: The present in vitro study evaluated the secondary caries resistance potential of acid-etched human coronal dentin bonded using augmented pressure adhesive displacement in conjunction with an experimental antibacterial adhesive. One hundred and twenty class I cavities were restored with a commercial non-antibacterial etch-and-rinse adhesive (N) or an experimental antibacterial adhesive (A) which was displaced by gentle air-blow (G) or augmented pressure air-blow (H). After bonding and restoration with resin composite, the resulted 4 groups (N-G, N-H, A-G and A-H) were exposed to Streptococcus mutans biofilm for 4, 8, 15, 20 or 25 days. The development of secondary caries in the bonding interface was then examined by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Data acquired from 15, 20 and 25 days of artificial caries induction were analyzed with three-way ANOVA at α = 0.05. The depth of the artificial carious lesions was significantly affected by "adhesive type" (Single Bond 2 vs experimental antibacterial adhesive p = 0.003), "intensity of adhesive displacement" (gentle vs augmented-pressure adhesive displacement; p < 0.001), as well as "artificial caries induction time" (p < 0.001). The combined use of augmented pressure adhesive displacement and experimental antibacterial adhesive reduces the progression of secondary caries.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of General Dentistry and Emergency, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Shen
authors.affiliation: Department of Endodontics, College of Dental Medicine, Augusta University, Augusta, Georgia, USA.
authors.forename: Franklin R
authors.initials: FR
authors.lastname: Tay
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Ji-hua
authors.initials: JH
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D003731:Dental Caries / Q000209:etiology / Q000517:prevention & control*; D017220:Dentin-Bonding Agents / Q000627:therapeutic use*; D006801:Humans; D066298:In Vitro Techniques; D008963:Molar / Q000187:drug effects* / Q000382:microbiology; D012008:Recurrence; D013290:Streptococcal Infections / Q000150:complications / Q000188:drug therapy*; D013295:Streptococcus mutans
chemical_list: D000900:Anti-Bacterial Agents; D017220:Dentin-Bonding Agents