title: Cyclic adenosine monophosphate-protein kinase A signal pathway may be involved in pulmonary aquaporin-5 expression in ischemia/reperfusion rats following deep hypothermia cardiac arrest.
abstract: We investigated the effects of cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) on the expression of aquaporin 5 (AQP5) in ischemia/reperfusion (I/R) rats following deep hypothermia cardiac arrest. Wistar rats were randomly divided into: a sham control group (subjected to a sham operation); an I/R group (subjected to occlusion of the bronchial arteries and the left inferior pulmonary artery); an H89 group (subjected to occlusion of the bronchial arteries and the left inferior pulmonary vein and artery, and treated with 5 mg/kg H89 for 2 days before the study); and a forskolin group (subjected to occlusion of the bronchial arteries and the left inferior pulmonary vein and artery, and treated with 5 mg/kg forskolin for 2 days before the study). Expression levels of AQP5 mRNA and protein were determined using reverse transcription-polymerase chain reaction and western blotting. Decreased expression of AQP5 was noted in the pulmonary tissues of the I/R group compared with the sham controls. Compared to that in the control group, there was a notable decrease in AQP5 expression in the I/R group. After treating with forskolin, AQP5 expression increased in the forskolin group compared with the I/R group. In the H89 group, AQP5 expression decreased compared with the I/R group. The decreased expression of AQP5 was possibly associated with acute pulmonary injury induced by I/R. The cAMP-PKA signal pathway may be involved in the expression of AQP5 in I/R rats after deep hypothermia cardiac arrest.
authors.affiliation: Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: Division of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: R
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: F F
authors.initials: FF
authors.lastname: Dai
authors.affiliation: Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Z F
authors.initials: ZF
authors.lastname: Mao
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology* / Q000378:metabolism; D000818:Animals; D051402:Aquaporin 5 / Q000235:genetics*; D017868:Cyclic AMP-Dependent Protein Kinases; D004195:Disease Models, Animal; D015536:Down-Regulation; D006323:Heart Arrest / Q000150:complications*; D007035:Hypothermia; D007511:Ischemia / Q000150:complications / Q000209:etiology; D008168:Lung / Q000098:blood supply* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051402:Aquaporin 5; D017868:Cyclic AMP-Dependent Protein Kinases