title: Ectonucleotidases and adenosine deaminase activity in laying hens naturally infected by Salmonella Gallinarum and their effects on the pathogenesis of the disease.
abstract: Salmonella Gallinarum is the etiologic agent of fowl typhoid that affects chickens and turkeys causing egg production drops, infertility, lower hatchability, high mortality, and as a consequence severe economic losses to the poultry industry. The alterations in NTPDase and adenosine deaminase (ADA) activities have been demonstrated in several inflammatory conditions; however, there are no data in the literature associated with this infection. Thus, the aim of this study was to evaluate the activities of NTPDase, 5'nucleotidase, and ADA in serum and hepatic tissue of laying hens naturally infected by Salmonella Gallinarum. Liver and serum samples were collected of 27 laying hens (20 S. Gallinarum infected and 7 uninfected). NTPDase and 5'-nucleotidase activities in serum were increased (P < 0.001) in infected animals to hydrolysis of substrate ATP, ADP and AMP. In addition, it was observed decreased (P < 0.001) in ADA activity in serum of laying hens naturally infected by S. Gallinarum; as well as increased (P < 0.001) ADA activity in liver tissue of infected laying hens. Histopathological analyses revealed that S. Gallinarum caused fibrinoid necrosis in liver and spleen associated with infiltrates of heterophils, macrophages, lymphocytes, and plasma cells. Considering that NTPDase and ADA are involved in the cell-mediated immunity, this study suggests that activities of these enzymes could be important biomarkers to determine the severity of inflammatory and immune responses in salmonellosis, contributing to clarify the pathogenesis of the disease.
authors.affiliation: Animal Science Department, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil. Electronic address: mmboiago@gmail.com.
authors.forename: Marcel M
authors.initials: MM
authors.lastname: Boiago
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil; Microbiology and Parasitology Department, UFSM, Santa Maria, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Pedro H
authors.initials: PH
authors.lastname: Doleski
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Guilherme M
authors.initials: GM
authors.lastname: do Carmo
authors.affiliation: Animal Science Department, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil.
authors.forename: Denise N
authors.initials: DN
authors.lastname: Araujo
authors.affiliation: Animal Science Graduate Program, UDESC, Chapecó, Brazil.
authors.forename: Jessica
authors.initials: J
authors.lastname: Giuriatti
authors.affiliation: Animal Science Department, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Baggio
authors.affiliation: Microbiology and Parasitology Department, UFSM, Santa Maria, Brazil.
authors.forename: Daniela B R
authors.initials: DB
authors.lastname: Leal
authors.affiliation: Animal Science Graduate Program, UDESC, Lages, Brazil.
authors.forename: Renata A
authors.initials: RA
authors.lastname: Casagrande
authors.affiliation: Animal Science Graduate Program, UDESC, Lages, Brazil.
authors.forename: Cláudia S
authors.initials: CS
authors.lastname: Wisser
authors.affiliation: Animal Science Department, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil.
authors.forename: Lenita M
authors.initials: LM
authors.lastname: Stefani
authors.affiliation: Animal Science Department, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil; Biochemistry and Molecular Biology Department, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil; Animal Science Graduate Program, UDESC, Chapecó, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: da Silva
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics / Q000276:immunology*; D000818:Animals; D002645:Chickens / Q000382:microbiology; D005260:Female; D007111:Immunity, Cellular; D008099:Liver / Q000382:microbiology / Q000473:pathology; D009708:Nucleotidases / Q000235:genetics / Q000276:immunology*; D011201:Poultry Diseases / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D012481:Salmonella Infections, Animal / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D019779:Salmonella enterica / Q000502:physiology*; D013154:Spleen / Q000382:microbiology / Q000473:pathology
chemical_list: D009708:Nucleotidases; C030287:nucleotidase; D000243:Adenosine Deaminase
keywords: ADA; NTPDase; Pathology; Salmonellosis