title: Methanotrophic production of polyhydroxybutyrate-co-hydroxyvalerate with high hydroxyvalerate content.
abstract: Type II methanotrophic bacteria are a promising production platform for PHA biopolymers. These bacteria are known to produce pure poly-3-hydroxybutyrate homopolymer (PHB). We isolated a strain, Methylocystis sp. WRRC1, that was capable of producing a wide range of polyhydroxybutyrate-co-hydroxyvalerate copolymers (PHB-co-HV) when co-fed methane and valerate or n-pentanol. The ratio of HB to HV monomer was directly related to the concentration of valeric acid in the PHA accumulation media. We observed increased incorporation of HV and total polymer under copper-free growth conditions. The PHB-co-HV copolymers produced had decreased melting temperatures and crystallinity compared with methanotroph-produced PHB.
authors.affiliation: USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Cal
authors.affiliation: USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States.
authors.forename: W Dirk
authors.initials: WD
authors.lastname: Sikkema
authors.affiliation: USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Ponce
authors.affiliation: USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States.
authors.forename: Diana
authors.initials: D
authors.lastname: Franqui-Villanueva
authors.affiliation: USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Riiff
authors.affiliation: USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States.
authors.forename: William J
authors.initials: WJ
authors.lastname: Orts
authors.affiliation: Mango Materials, 490 Lake Park Ave #16098, Oakland, CA 94610, United States.
authors.forename: Allison J
authors.initials: AJ
authors.lastname: Pieja
authors.affiliation: USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States. Electronic address: Charles.Lee@ars.usda.gov.
authors.forename: Charles C
authors.initials: CC
authors.lastname: Lee
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D003300:Copper / Q000494:pharmacology; D008697:Methane / Q000378:metabolism; D042083:Methylocystaceae / Q000187:drug effects / Q000378:metabolism*; D010421:Pentanoic Acids / Q000378:metabolism; D000439:Pentanols / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
chemical_list: D003300:Copper; D008697:Methane; D010421:Pentanoic Acids; D000439:Pentanols; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C038780:n-pentanoic acid
keywords: Copolymer; Methanotroph; Polyhydroxyalkanoate