title: Calcium imaging of sleep-wake related neuronal activity in the dorsal pons.
abstract: The dorsal pons has long been implicated in the generation of rapid eye movement (REM) sleep, but the underlying circuit mechanisms remain poorly understood. Using cell-type-specific microendoscopic Ca(2+) imaging in and near the laterodorsal tegmental nucleus, we found that many glutamatergic neurons are maximally active during REM sleep (REM-max), while the majority of GABAergic neurons are maximally active during wakefulness (wake-max). Furthermore, the activity of glutamatergic neurons exhibits a medio-lateral spatial gradient, with medially located neurons more selectively active during REM sleep.
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, 230 Li Ka Shing Center, Berkeley, California 94720, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Cox
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, 230 Li Ka Shing Center, Berkeley, California 94720, USA.
authors.forename: Lucas
authors.initials: L
authors.lastname: Pinto
authors.affiliation: Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, Howard Hughes Medical Institute, University of California, 230 Li Ka Shing Center, Berkeley, California 94720, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dan
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D016000:Cluster Analysis; D004569:Electroencephalography; D004576:Electromyography; D059330:GABAergic Neurons / Q000378:metabolism* / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D061848:Optical Imaging; D065821:Pontine Tegmentum / Q000378:metabolism* / Q000502:physiology; D012890:Sleep / Q000502:physiology; D012895:Sleep, REM / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology*
chemical_list: D018698:Glutamic Acid; D002118:Calcium