title: Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles.
abstract: We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia. romanov@cardio.ru.|CryoCenter Umbilical Cord Blood Bank, Moscow, Russia. romanov@cardio.ru.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Romanov
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.|CryoCenter Umbilical Cord Blood Bank, Moscow, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Balashova
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N E
authors.initials: NE
authors.lastname: Volgina
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Kabaeva
authors.affiliation: CryoCenter Umbilical Cord Blood Bank, Moscow, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Dugina
authors.affiliation: V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D002470:Cell Survival; D015925:Cryopreservation; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D014470:Umbilical Cord / Q000166:cytology
keywords: cryogenic storage; hematopoietic stem cell; repeated freezing/thawing cycles; umbilical blood; viability