title: Bacteria counting method based on polyaniline/bacteria thin film.
abstract: A simple and rapid bacteria counting method based on polyaniline (PANI)/bacteria thin film was proposed. Since the negative effects of immobilized bacteria on the deposition of PANI on glass carbon electrode (GCE), PANI/bacteria thin films containing decreased amount of PANI would be obtained when increasing the bacteria concentration. The prepared PANI/bacteria film was characterized with cyclic voltammetry (CV) technique to provide quantitative index for the determination of the bacteria count, and electrochemical impedance spectroscopy (EIS) was also performed to further investigate the difference in the PANI/bacteria films. Good linear relationship of the peak currents of the CVs and the log total count of bacteria (Bacillus subtilis) could be established using the equation Y=-30.413X+272.560 (R(2)=0.982) over the range of 5.3×10(4) to 5.3×10(8)CFUmL(-1), which also showed acceptable stability, reproducibility and switchable ability. The proposed method was feasible for simple and rapid counting of bacteria.
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhihua
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Xuetao
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Jiyong
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: zou_xiaobo@ujs.edu.cn.
authors.forename: Zou
authors.initials: Z
authors.lastname: Xiaobo
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Huang
authors.initials: H
authors.lastname: Xiaowei
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xucheng
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Haroon Elrasheid
authors.initials: HE
authors.lastname: Tahir
authors.affiliation: School of Food Science and Nutrition, the University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: Mel
authors.initials: M
authors.lastname: Holmes
authors.affiliation: School of Food Science and Nutrition, the University of Leeds, Leeds LS2 9JT, United Kingdom.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Povey
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D001419:Bacteria / Q000166:cytology*; D058491:Bacterial Load / Q000379:methods*; D018407:Bacterial Physiological Phenomena; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D058266:Dielectric Spectroscopy; D004566:Electrodes; D004595:Electroplating / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D000814:Aniline Compounds; C416807:polyaniline; D002244:Carbon
keywords: Bacteria counting; Polyaniline; Thin film