title: Application of new phenolic antioxidants for cryopreservation of sturgeon sperm.
abstract: Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects of BHT and water-soluble analogue of vitamin E, trolox. The most efficient antioxidant has the effective concentration of 0.1 mM. Novel antioxidant agents as cryomedium supplements not only reduced the level of lipid peroxidation, but also enhanced the translational motility of the sperm of the Russian sturgeon after defrosting.
authors.affiliation: Southern Scientific Center, Russian Academy of Sciences, st. Chehova, 41, Rostov-on-Don, 344006, Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Osipova
authors.affiliation: Astrakhan State Technical University, ul. Tatishcheva 16, Astrakhan, 414025, Russia.
authors.forename: N T
authors.initials: NT
authors.lastname: Berberova
authors.affiliation: North Ossetian State University, Vladikavkaz, 362040, Russia.
authors.forename: R A
authors.initials: RA
authors.lastname: Gazzaeva
authors.affiliation: Lomonosov Moscow State University, Leninskie gory 1/3, Moscow, 119991, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Kudryavtsev
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000378:metabolism / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005399:Fishes / Q000502:physiology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010636:Phenols / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D002839:Chromans; D003451:Cryoprotective Agents; D010636:Phenols; D002084:Butylated Hydroxytoluene; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
keywords: Antioxidant; Cryopreservation; Lipid peroxidation; Sterically hindered phenol; The sperm of the Russian sturgeon