title: New bioactive labdane diterpenoids from Marrubium aschersonii.
abstract: A phytochemical investigation of the ethanol extract of Marrubium aschersonii Magnus (Lamiaceae) collected from Tunisia led to the isolation and identification of two new labdane diterpenoids, marrubaschs A (1) and B (2), along with two known compounds (3 and 4). Their structures were elucidated by spectroscopic methods including HRESIMS and NMR techniques. All compounds were evaluated for their inhibitory effects on the nitric oxide (NO) production induced by lipopolysaccharide in RAW 264.7 macrophage cells. Compound 2 exhibited weak inhibition of NO production with an IC50 value of 35 ± 1.0 μM.
authors.affiliation: a Research Unit Applied Chemistry and Environment 13ES63, Faculty of Sciences of Monastir , Monastir University , Monastir , Tunisia.
authors.forename: Saoussen
authors.initials: S
authors.lastname: Hammami
authors.affiliation: b School of Pharmaceutical Sciences , Sun Yat-sen University , Guangzhou , China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: b School of Pharmaceutical Sciences , Sun Yat-sen University , Guangzhou , China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Huang
authors.affiliation: c Laboratory of Botany and plant Ecology, Faculty of Sciences , University of Bizerta , Jarzouna, Bizerta , Tunisia.
authors.forename: Ridha
authors.initials: R
authors.lastname: El Mokni
authors.affiliation: a Research Unit Applied Chemistry and Environment 13ES63, Faculty of Sciences of Monastir , Monastir University , Monastir , Tunisia.
authors.forename: Hatem
authors.initials: H
authors.lastname: Dhaouadi
authors.affiliation: b School of Pharmaceutical Sciences , Sun Yat-sen University , Guangzhou , China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yin
mesh_terms: D000818:Animals; D002460:Cell Line; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005663:Furans / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D027522:Marrubium / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry; D014416:Tunisia
chemical_list: D004224:Diterpenes; D005663:Furans; D008070:Lipopolysaccharides; D010936:Plant Extracts; C000619172:marrubasch B; C473344:marrubenol; D009569:Nitric Oxide; C105731:marrubiin
keywords: Marrubium aschersonii; anti-inflammatory activities; labdane diterpenoids