title: The enhanced anti-tissue adhesive effect of injectable pluronic-HA hydrogel by poly(γ-glutamic acid).
abstract: The stability of tissue barrier in physiological condition is a key factor to isolate the damaged site from adjacent tissue for anti-tissue adhesion. Although pluronic or pluronic-hyaluronic acid (HA) hydrogel as an injectable formulation can prevent tissue adhesion at the injection site, the anti-tissue adhesion effect is limited due to its poor stability. Herein, we prepared tissue barrier formulations composed of pluronic F127 (F127) and HA mixture (F127-HA) and the effect of the addition of poly(γ-glutamic acid) (PGA) was characterized. All of F127, HA, and F127-HA mixture showed the poor in vitro residence stability less than 3 days. However, by adding PGA into F127-HA mixture, their stability was significantly enhanced by the control of the molecular weight and concentration of PGA. Thus, F127-HA with 10wt% PGA (2000kDa) showed the long-term stability over 10 days. Similarly, the enhanced stability of F127-HA with PGA resulted in the enhanced and excellent in vivo anti-tissue adhesion effect, evidenced by histological analysis and grading of tissue adhesion. Therefore, F127-HA containing PGA could be applied as an efficient injectable tissue barrier for anti-tissue adhesion.
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 61005, Republic of Korea.
authors.forename: Manse
authors.initials: M
authors.lastname: Kim
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 61005, Republic of Korea.
authors.forename: Youngmin
authors.initials: Y
authors.lastname: Hwang
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, 61005, Republic of Korea. Electronic address: gytae@gist.ac.kr.
authors.forename: Giyoong
authors.initials: G
authors.lastname: Tae
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D055119:Elastic Modulus; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D020442:Poloxamer / Q000737:chemistry; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D014014:Tissue Adhesives / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D014014:Tissue Adhesives; C511775:poly(gamma-glutamic acid); D020442:Poloxamer; D011099:Polyglutamic Acid; D006820:Hyaluronic Acid
keywords: Hyaluronic acid; Hydrogel; Pluronic F127; Poly(γ-glutamic acid); Tissue barrier