title: Development and validation of an LC-MS/MS method for simultaneous quantification of levodopa and MD01 in rat plasma and its application to a pharmacokinetic study of mucuna pruriens extract.
abstract: Mucuna pruriens, an ancient Indian herbal medicine containing levodopa, is widely used for Parkinson's disease. In order to simultaneously determine levodopa and 1,1-dimethyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (MD01) in rat plasma, an improved LC-MS/MS method was developed and validated for a pharmacokinetic study in rats orally administered levodopa or Mucuna pruriens extract (MPE). Elimination of matrix effect and improvement of extraction recovery were achieved through systematic optimization of reversed-phase and hydrophilic interaction chromatographic conditions together with sample clean-up procedures. A satisfactory chromatographic performance was obtained with a Thermo Aquasil C18 column (50 × 2.1 mm, 3 µm) using acetonitrile and water containing 0.2% formic acid as mobile phases. Futhermore, sodium metabisulfite and formic acid were used as stabilizers in neat solutions as well as rat plasma. The method was validated in a dynamic range of 20.0-10,000 ng/mL for levodopa and MD01; the intra- and inter-day precision and accuracy were acceptable. The method was successfully utilized to determine the levodopa level in plasma samples of rats administered levodopa or MPE. Pharmacokinetic results showed that an increase in the AUC of levodopa was observed in rats following oral administration of multiple doses of MPE. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Scinence and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, China.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Yang
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Scinence and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, China.
authors.forename: Fangrong
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Linfang
authors.initials: L
authors.lastname: Deng
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Scinence and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Chen
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Scinence and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, China.|School of Pharmacy, Weifang Medical University, Weifang, 261053, Shandong, China.
authors.forename: Zhongzhe
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Scinence and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, China.
authors.forename: Jiangeng
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.
authors.forename: Jiangyun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Scinence and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D007546:Isoquinolines / Q000097:blood* / Q000493:pharmacokinetics; D007980:Levodopa / Q000097:blood* / Q000493:pharmacokinetics; D031299:Mucuna / Q000737:chemistry*; D010936:Plant Extracts / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007546:Isoquinolines; D010936:Plant Extracts; D007980:Levodopa