title: Comparative transcript profiling of resistant and susceptible peanut post-harvest seeds in response to aflatoxin production by Aspergillus flavus.
abstract: BACKGROUND
  Aflatoxin contamination caused by Aspergillus flavus in peanut (Arachis hypogaea) including in pre- and post-harvest stages seriously affects industry development and human health. Even though resistance to aflatoxin production in post-harvest peanut has been identified, its molecular mechanism has been poorly understood. To understand the mechanism of peanut response to aflatoxin production by A. flavus, RNA-seq was used for global transcriptome profiling of post-harvest seed of resistant (Zhonghua 6) and susceptible (Zhonghua 12) peanut genotypes under the fungus infection and aflatoxin production stress.
  
  
  RESULTS
  A total of 128.72 Gb of high-quality bases were generated and assembled into 128, 725 unigenes (average length 765 bp). About 62, 352 unigenes (48.43%) were annotated in the NCBI non-redundant protein sequences, NCBI non-redundant nucleotide sequences, Swiss-Prot, KEGG Ortholog, Protein family, Gene Ontology, or eukaryotic Ortholog Groups database and more than 93% of the unigenes were expressed in the samples. Among obtained 30, 143 differentially expressed unigenes (DEGs), 842 potential defense-related genes, including nucleotide binding site-leucine-rich repeat proteins, polygalacturonase inhibitor proteins, leucine-rich repeat receptor-like kinases, mitogen-activated protein kinase, transcription factors, ADP-ribosylation factors, pathogenesis-related proteins and crucial factors of other defense-related pathways, might contribute to peanut response to aflatoxin production. Notably, DEGs involved in phenylpropanoid-derived compounds biosynthetic pathway were induced to higher levels in the resistant genotype than in the susceptible one. Flavonoid, stilbenoid and phenylpropanoid biosynthesis pathways were enriched only in the resistant genotype.
  
  
  CONCLUSIONS
  This study provided the first comprehensive analysis of transcriptome of post-harvest peanut seeds in response to aflatoxin production, and would contribute to better understanding of molecular interaction between peanut and A. flavus. The data generated in this study would be a valuable resource for genetic and genomic studies on crops resistance to aflatoxin contamination.
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. wanghoumiao@163.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. wanghoumiao@163.com.
authors.forename: Houmiao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. leiyong@caas.cn.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. leiyong@caas.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. wanliyun@caas.cn.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. wanliyun@caas.cn.
authors.forename: Liyun
authors.initials: L
authors.lastname: Wan
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. yanliying2002@126.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. yanliying2002@126.com.
authors.forename: Liying
authors.initials: L
authors.lastname: Yan
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. lvjianwei_163@163.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. lvjianwei_163@163.com.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Lv
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. daixiaofeng@caas.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Dai
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. renxp1972@hotmail.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. renxp1972@hotmail.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ren
authors.affiliation: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. iewguo@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. peanutlab@iolcrops.cn.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. peanutlab@iolcrops.cn.
authors.forename: Huifang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. liaoboshou@163.com.|Chinese Academy of Agricultural Sciences-International Crop Research Institute for the Semi-Arid Tropics Joint Laboratory for Groundnut Aflatoxin Management, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. liaoboshou@163.com.
authors.forename: Boshou
authors.initials: B
authors.lastname: Liao
mesh_terms: D000348:Aflatoxins; D010367:Arachis / Q000235:genetics* / Q000382:microbiology; D001231:Aspergillus flavus / Q000502:physiology*; D018556:Crops, Agricultural / Q000235:genetics; D017343:Genes, Plant; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D012639:Seeds / Q000235:genetics; D059467:Transcriptome
chemical_list: D000348:Aflatoxins