title: Enzymatic network for production of ether amines from alcohols.
abstract: We constructed an enzymatic network composed of three different enzymes for the synthesis of valuable ether amines. The enzymatic reactions are interconnected to catalyze the oxidation and subsequent transamination of the substrate and to provide cofactor recycling. This allows production of the desired ether amines from the corresponding ether alcohols with inorganic ammonium as the only additional substrate. To examine conversion, individual and overall reaction equilibria were established. Using these data, it was found that the experimentally observed conversions of up to 60% observed for reactions containing 10 mM alcohol and up to 280 mM ammonia corresponded well to predicted conversions. The results indicate that efficient amination can be driven by high concentrations of ammonia and may require improving enzyme robustness for scale-up. Biotechnol. Bioeng. 2016;113: 1853-1861. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 4, Groningen, 9747AG, The Netherlands.
authors.forename: Cyntia M
authors.initials: CM
authors.lastname: Palacio
authors.affiliation: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 4, Groningen, 9747AG, The Netherlands.
authors.forename: Ciprian G
authors.initials: CG
authors.lastname: Crismaru
authors.affiliation: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 4, Groningen, 9747AG, The Netherlands.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Bartsch
authors.affiliation: White Biotechnology Research, BASF SE, Ludwigshafen, Germany.
authors.forename: Vaidotas
authors.initials: V
authors.lastname: Navickas
authors.affiliation: White Biotechnology Research, BASF SE, Ludwigshafen, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Ditrich
authors.affiliation: White Biotechnology Research, BASF SE, Ludwigshafen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Breuer
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
authors.forename: Rohana
authors.initials: R
authors.lastname: Abu
authors.affiliation: Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark.
authors.forename: John M
authors.initials: JM
authors.lastname: Woodley
authors.affiliation: White Biotechnology Research, BASF SE, Ludwigshafen, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Baldenius
authors.affiliation: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 4, Groningen, 9747AG, The Netherlands.
authors.forename: Bian
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Nijenborgh 4, Groningen, 9747AG, The Netherlands. d.b.janssen@rug.nl.
authors.forename: Dick B
authors.initials: DB
authors.lastname: Janssen
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000438:Alcohols / Q000737:chemistry / Q000378:metabolism*; D000588:Amines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000378:metabolism; D055162:Biocatalysis; D004986:Ether / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000637:Transaminases / Q000378:metabolism*
chemical_list: D000438:Alcohols; D000588:Amines; D001426:Bacterial Proteins; D004986:Ether; D000426:Alcohol Dehydrogenase; D000637:Transaminases
keywords: alcohol dehydrogenase; amination; aminotransferase; biocatalysis; ether amines