title: Inhibiting effect of Endostar combined with ginsenoside Rg3 on breast cancer tumor growth in tumor-bearing mice.
abstract: OBJECTIVE
  To study the inhibiting effect of Endostar combined with ginsenoside Rg3 on breast cancer tumor growth in tumor-bearing mice.
  
  
  METHODS
  Female mice were selected as experimental animals, and breast cancer tumor-bearing mouse models were established and then divided into groups A, B, C and D that respectively received saline, recombinant human endostatin, ginsenosides Rg3 and recombinant human endostatin combined with Rg3 intervention; 7 d, 14 d and 21 d after intervention, tumor tissue volume was measured; 21 d after intervention, mice were killed, tumor tissue was collected, and mRNA contents of angiogenesis molecules, invasion molecules, autophagy marker molecules and autophagy signaling pathway molecules were detected.
  
  
  RESULTS
  At 7 d, 14 d and 21 d after intervention, tumor tissue volume of groups B, C and D was lower than that of group A, and tumor tissue volume of group D was lower than that of groups B and C; mRNA contents of VEGFA, VEGFB, VEGFC, MMP2, MMP9, p62, mTOR, PI3K, Akt, JNK and Beclin-1 in tumor tissue of groups B, C and D were significantly lower than those of group A, and LC3-II/LC3-I was significantly higher than that of group A; mRNA contents of VEGFA, VEGFB, VEGFC, MMP2, MMP9, p62, mTOR, PI3K, Akt, JNK and Beclin-1 in tumor tissue of group D were significantly lower than those of groups B and C, and LC3-II/LC3-I was higher than that of groups B and C.
  
  
  CONCLUSIONS
  Endostar combined with ginsenoside Rg3 has stronger inhibiting effect on breast cancer tumor growth in tumor-bearing mice than single drug, and it can inhibit angiogenesis and cell invasion, and enhance cell autophagy.
authors.affiliation: Oncology Department No. 2, Linyi People's Hospital, Linyi City, Shandong Province, 276000, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Radiotherapy Department, Linyi People's Hospital, Linyi City, Shandong Province, 276000, China.
authors.forename: Qing-Zhan
authors.initials: QZ
authors.lastname: Liu
authors.affiliation: Oncology Department No. 2, Linyi People's Hospital, Linyi City, Shandong Province, 276000, China. Electronic address: fenglin_w@126.com.
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Xing
authors.affiliation: Oncology Department No. 2, Linyi People's Hospital, Linyi City, Shandong Province, 276000, China.
authors.forename: Jin-Ling
authors.initials: JL
authors.lastname: Zhang
keywords: Autophagy; Breast cancer; Ginsenoside Rg3; Recombinant human endostatin