title: Catechin Hydrate Augments the Antibacterial Action of Selected Antibiotics against Staphylococcus aureus Clinical Strains.
abstract: Synergistic effects between commonly used antibiotics and natural substances may be an alternative to conventional antibacterial therapies. The objective of the presented study was to assess the in vitro antibacterial activity of catechin hydrate (CH) and evaluate the interactions of CH with selected antibiotics using Staphylococcus aureus clinical and reference strains. CH displayed diverse activity towards examined S. aureus strains, with minimal inhibitory concentrations (MICs) ranging from 256 to 2048 µg/mL. The interaction between CH and antibiotics was assessed by an E-test. The most significant synergistic effects were noticed for CH in combination with clindamycin and erythromycin. For cefoxitin and vancomycin a decrease of MIC values in the presence of CH was also observed, but it did not reach statistical significance. The obtained results demonstrate that CH shows antimicrobial activity against Staphylococcus aureus clinical strains. What is more, we proved a synergistic effect of CH with erythromycin and clindamycin.
authors.affiliation: Department of Microbiology and Virology, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Jagiellońska 4, 41-200 Sosnowiec, Poland. maria.miklasinska@o2.pl.
authors.forename: Maria
authors.initials: M
authors.lastname: Miklasińska
authors.affiliation: Department of Microbiology and Virology, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Jagiellońska 4, 41-200 Sosnowiec, Poland. mkepa@sum.edu.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kępa
authors.affiliation: Department of Microbiology and Virology, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Jagiellońska 4, 41-200 Sosnowiec, Poland. rwojtyczka@sum.edu.pl.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Wojtyczka
authors.affiliation: Department of Microbiology and Virology, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Jagiellońska 4, 41-200 Sosnowiec, Poland. didzik@sum.edu.pl.
authors.forename: Danuta
authors.initials: D
authors.lastname: Idzik
authors.affiliation: Department of Conservative Dentistry with Endodontics, Medical University of Silesia, Katowice, Pl. Akademicki 17, 41-902 Bytom, Poland. adziedzic@sum.edu.pl.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Dziedzic
authors.affiliation: Department of Microbiology and Virology, School of Pharmacy and Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ul. Jagiellońska 4, 41-200 Sosnowiec, Poland. twasik@sum.edu.pl.
authors.forename: Tomasz J
authors.initials: TJ
authors.lastname: Wąsik
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002981:Clindamycin / Q000494:pharmacology; D004357:Drug Synergism; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin; D014640:Vancomycin; D002392:Catechin
keywords: Staphylococcus aureus; antibacterial activity; catechin hydrate