title: Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson.
abstract: Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes α-pinene (63.4 - 68.5%) and β-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that α-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 ± 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic and caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 μg/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 μg/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 μg/ml).
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Mirjana D
authors.initials: MD
authors.lastname: Marčetić
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Marina T
authors.initials: MT
authors.lastname: Milenković
authors.affiliation: Institute of Botany and Botanical Garden 'Jevremovac', Faculty of Biology, University of Belgrade, Takovska 43, RS-11000, Belgrade.
authors.forename: Dmitar V
authors.initials: DV
authors.lastname: Lakušić
authors.affiliation: Department of Botany, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Branislava S
authors.initials: BS
authors.lastname: Lakušić
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D020902:Hypericum / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile; D010936:Plant Extracts; D000432:Methanol
keywords: Antimicrobial activity; Essential oils; Hypericum aegypticum subsp. webbii; Methanol extracts; SIMPER Analysis