title: [Methylation of miR-378 in Chronic Myeloid Leukemia].
abstract: OBJECTIVE
  To investigate the methylation status of miR-378 promoter in chronic myeloid leukemia (CML) and to analyze its clinical significance.
  
  
  METHODS
  The unmethylation level of miR-378 gene promoter in bone marrow mononuclear cells of 25 healthy donors and 53 patients with CML was detected by using real-time quantitative methylation-specific PCR (RQ-MSP).
  
  
  RESULTS
  The hypomethylation of miR-378 gene promoter was found in 17/53 (32.1%) patients, but only in 1/25 (4.0%) of controls. The difference between the two groups was very statistically significant (P < 0.01). The frequency of miR-378 unmethylation in CML patients at chronic phase (CP), accelerated phase (AP) and blastic phase (BP) was 35.0% (14/40), 40.0% (2/5), and 12.5% (1/8), respectively. However, there were no significant differences in the unmethylation level of miR-378 among CML patients at different sexes, stages and karyotypes. No significant differences could be observed in age, white blood cell counts, platelet count, hemoglobin level and BCR/ABL1 transcript level (P > 0.05). CONCLUDSION: The miR-378 hypomethylation is a common molecular event in CML, especially at chronic or accelerated phases.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.|Department of Hematology, The Third People's Hospital of Kunshan City, Jiangsu Province, Kunshang 215316, China.
authors.forename: De-Hong
authors.initials: DH
authors.lastname: Wu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Xiang-Mei
authors.initials: XM
authors.lastname: Wen
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Clinical Laboratorial Examination, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Yao
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Central Laboratory, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China.
authors.forename: Zhao-Qun
authors.initials: ZQ
authors.lastname: Deng
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Jiangsu University, Zhengjiang 212002, Jiangsu Province, China. E-mail: qianjun0007@sina.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism; D016022:Case-Control Studies; D019175:DNA Methylation; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D035683:MicroRNAs / Q000378:metabolism*; D011401:Promoter Regions, Genetic
chemical_list: C526012:MIRN378 microRNA, human; D035683:MicroRNAs