title: Ultra-High-Throughput Sample Preparation System for Lymphocyte Immunophenotyping Point-of-Care Diagnostics.
abstract: Point-of-care (POC) microfluidic devices often lack the integration of common sample preparation steps, such as preconcentration, which can limit their utility in the field. In this technology brief, we describe a system that combines the necessary sample preparation methods to perform sample-to-result analysis of large-volume (20 mL) biopsy model samples with staining of captured cells. Our platform combines centrifugal-paper microfluidic filtration and an analysis system to process large, dilute biological samples. Utilizing commercialization-friendly manufacturing methods and materials, yielding a sample throughput of 20 mL/min, and allowing for on-chip staining and imaging bring together a practical, yet powerful approach to microfluidic diagnostics of large, dilute samples.
authors.affiliation: Science for Life Laboratory, Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, Stockholm, Sweden Department of Bioengineering, Northeastern University, Boston, MA, USA.
authors.forename: David I
authors.initials: DI
authors.lastname: Walsh
authors.affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA, USA Barnett Institute of Chemical and Biological Analysis, Northeastern University, Boston, MA, USA.
authors.forename: Shashi K
authors.initials: SK
authors.lastname: Murthy
authors.affiliation: Science for Life Laboratory, Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, Stockholm, Sweden aman.russom@scilifelab.se.
authors.forename: Aman
authors.initials: A
authors.lastname: Russom
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D019095:Point-of-Care Systems; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*
keywords: cell capture; cell immunophenotyping; centrifugal microfluidics; paper-based microfluidics; sample preparation