title: Preparation and cytotoxicity of N-modified chitosan nanoparticles applied in curcumin delivery.
abstract: Nanoparticles (NPs) based on N,N-dimethyl chitosan (DMC) and N,N,N-trimethyl chitosan (TMC), physical crosslinked with sodium tripolyphosphate (TPP) were successful obtained, using water/benzyl alcohol emulsion system. NPs morphologies were evaluated by Scanning Electron Microscopy and Transmission Electron Microscopy. NPs were characterized by Infrared Spectroscopy (FTIR), Thermogravimetric Analysis, Zeta Potential, Differential Scanning Calorimetry and Wide-angle X-ray Scattering. Curcumin (CUR) was loaded onto NPs and controlled release studies were evaluated in simulated intestinal fluid and in simulated gastric fluid. Cytotoxicity assays showed only loaded TMC/TPP particles containing CUR were slightly cytotoxic on human cervical tumor cells (SiHa cells), concerning unloaded TMC/TPP particles. Conversely, loaded NPs (TMC/TPP/CUR and DMC/TPP/CUR), especially TMC/TPP/CUR sample presented greater biocompatibility toward healthy VERO cells than unloaded NPs (TMC/TPP and DMC/TPP).
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brasil.
authors.forename: Suelen P
authors.initials: SP
authors.lastname: Facchi
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil.
authors.forename: Débora B
authors.initials: DB
authors.lastname: Scariot
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil.
authors.forename: Pedro V A
authors.initials: PV
authors.lastname: Bueno
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Souza
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brasil.
authors.forename: Luana C
authors.initials: LC
authors.lastname: Figueiredo
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil.
authors.forename: Heveline D M
authors.initials: HD
authors.lastname: Follmann
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil.
authors.forename: Cátia S
authors.initials: CS
authors.lastname: Nunes
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brasil.
authors.forename: Johny P
authors.initials: JP
authors.lastname: Monteiro
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brasil.
authors.forename: Elton G
authors.initials: EG
authors.lastname: Bonafé
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil.
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
authors.affiliation: Departamento de Química, Universidade Tecnológica Federal do Paraná (UTFPR), Apucarana 86812-1200, Brasil; Grupo de Materiais Poliméricos e Compósitos, Universidade Estadual de Maringá (UEM), Maringá 87020-900, Brasil. Electronic address: afmartins50@yahoo.com.br.
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry* / Q000633:toxicity*; D002522:Chlorocebus aethiops; D003474:Curcumin / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity*; D065546:Drug Liberation; D004355:Drug Stability; D006801:Humans; D053758:Nanoparticles; D013696:Temperature; D014709:Vero Cells
chemical_list: D004337:Drug Carriers; D048271:Chitosan; D003474:Curcumin
keywords: Curcumin; Cytotoxicity; Delivery; N,N,N-Trimethyl chitosan; Nanoparticles