title: Enterocytozoon bieneusi Genotypes in Grazing Horses in China and their Zoonotic Transmission Potential.
abstract: In present study, 262 fecal specimens were collected from 12 groups of grazing horses in the Xinjiang Uyghur Autonomous Region of China. The specimens were subjected to PCR and sequencing analyses of the ribosomal internal transcribed spacer (ITS). The overall prevalence of E. bieneusi in horses was 30.9% (81/262). No significant differences in prevalence were observed between horses of different ages or sexes. Nineteen genotypes were identified: 15 known genotypes (BEB6, CHG19, CM6, CM7, CM8, CS-1, CS-4, D, EpbA, EbpC, G, horse1, horse2, O, and Peru8) and four new genotypes (XJH1-XJH4). Six of these genotypes were previously detected in humans: BEB6, D, EbpA, EbpC, O, and Peru8. Genotype EbpC was the most prevalent (21/81), followed by EpbA (20/81), BEB6 (9/81), CM6 (4/81), horse1 (4/81), O (4/81), G (3/81), CHG19 (2/81), CM7 (2/81), horse2 (2/81), and XJH1 (2/81), whereas the remaining eight genotypes were seen in one specimen each. In a phylogenetic analysis, 14 genotypes (65/81, 80.2%), excluding genotypes BEB6, CM7, horse2, XJH1, and XJH4, belonged to group 1, which have zoonotic potential. The high diversity in the E. bieneusi genotypes and their zoonotic potential suggest that grazing horses are a potential source of zoonotic infection in humans.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.|College of Animal Science, Tarim University, 1487 Tarim Road, Alar, 843300, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Qi
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.|Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu, 451450, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Jian
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Haiju
authors.initials: H
authors.lastname: Dong
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Changshen
authors.initials: C
authors.lastname: Ning
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D044822:Biodiversity; D002681:China / Q000453:epidemiology; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006736:Horses / Q000469:parasitology*; D006801:Humans; D016881:Microsporidiosis / Q000453:epidemiology* / Q000469:parasitology / Q000635:transmission* / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D017422:Sequence Analysis, DNA; D012737:Sex Factors; D015047:Zoonoses / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
keywords: Internal transcribed spacer gene; prevalence