title: An Electrochemiluminescence Immunosensor Based on Gold-Magnetic Nanoparticles and Phage Displayed Antibodies.
abstract: Using the multiple advantages of the ultra-highly sensitive electrochemiluminescence (ECL) technique, Staphylococcus protein A (SPA) functionalized gold-magnetic nanoparticles and phage displayed antibodies, and using gold-magnetic nanoparticles coated with SPA and coupled with a polyclonal antibody (pcAb) as magnetic capturing probes, and Ru(bpy)₃(2+)-labeled phage displayed antibody as a specific luminescence probe, this study reports a new way to detect ricin with a highly sensitive and specific ECL immunosensor and amplify specific detection signals. The linear detection range of the sensor was 0.0001~200 µg/L, and the limit of detection (LOD) was 0.0001 µg/L, which is 2500-fold lower than that of the conventional ELISA technique. The gold-magnetic nanoparticles, SPA and Ru(bpy)₃(2+)-labeled phage displayed antibody displayed different amplifying effects in the ECL immunosensor and can decrease LOD 3-fold, 3-fold and 20-fold, respectively, compared with the ECL immunosensors without one of the three effects. The integrated amplifying effect can decrease the LOD 180-fold. The immunosensor integrates the unique advantages of SPA-coated gold-magnetic nanoparticles that improve the activity of the functionalized capturing probe, and the amplifying effect of the Ru(bpy)₃(2+)-labeled phage displayed antibodies, so it increases specificity, interference-resistance and decreases LOD. It is proven to be well suited for the analysis of trace amounts of ricin in various environmental samples with high recovery ratios and reproducibility.
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. mxh0511@sohu.com.
authors.forename: Xihui
authors.initials: X
authors.lastname: Mu
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. billzytong@126.com.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Tong
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. qibin_huang@126.com.
authors.forename: Qibin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. lbfhyjy@sohu.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. liuzhw07@lzu.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. hlq70@163.com.
authors.forename: Lanqun
authors.initials: L
authors.lastname: Hao
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. yizhe2007@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Dong
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. zjp337@126.com.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China. g.ch.chuan@263.net.
authors.forename: Chuan
authors.initials: C
authors.lastname: Gao
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001435:Bacteriophages / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D049449:Luminescence; D058185:Magnetite Nanoparticles / Q000737:chemistry; D012276:Ricin / Q000302:isolation & purification*; D013205:Staphylococcal Protein A / Q000737:chemistry*
chemical_list: D000906:Antibodies; D058185:Magnetite Nanoparticles; D013205:Staphylococcal Protein A; D006046:Gold; D012276:Ricin
keywords: ECL immunosensor; Staphylococcus protein A; gold-magnetic nanoparticles; phage displayed antibody; ricin