title: Early expression of the receptor for advanced glycation end products in a toxic model produced by 6-hydroxydopamine in the rat striatum.
abstract: The receptor for advanced glycation end products (RAGE) is commonly involved in different neurodegenerative and inflammatory disorders. The cellular signaling associated to RAGE activation may occur upon binding to different ligands. In this study we investigated whether the toxic model produced by 6-hydroxydopamine (6-OHDA) in rats comprises early noxious responses related to RAGE-mediated signaling cascades. In order to explore a possible interaction between 6-OHDA and RAGE, affinity parameters of RAGE with 6-OHDA were estimated by different means. The possible binding sites of 6-OHDA with the VC1 homodimer for both rat and human RAGE were also modeled. Our results show that the striatal infusion of 6-OHDA recruits RAGE upregulation, as evidenced by an early expression of the receptor. 6-OHDA was also found to bind the VC1 homodimer, although its affinity was moderate when compared to other ligands. This work contributes to the understanding of the role of RAGE activation for 6-OHDA-induced neurotoxicity.
authors.affiliation: Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Mexico; Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, SSA, Mexico; Departamento de Ingeniería Eléctrica, Universidad Autónoma Metropolitana-Iztapalapa, Mexico.
authors.forename: Iris N
authors.initials: IN
authors.lastname: Serratos
authors.affiliation: Departamento de Ingeniería Eléctrica, Universidad Autónoma Metropolitana-Iztapalapa, Mexico.
authors.forename: Pilar
authors.initials: P
authors.lastname: Castellanos
authors.affiliation: Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Mexico.
authors.forename: Nina
authors.initials: N
authors.lastname: Pastor
authors.affiliation: Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: César
authors.initials: C
authors.lastname: Millán-Pacheco
authors.affiliation: Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, SSA, Mexico.
authors.forename: Ana Laura
authors.initials: AL
authors.lastname: Colín-González
authors.affiliation: Departamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Mexico.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rembao
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Ruy
authors.initials: R
authors.lastname: Pérez-Montfort
authors.affiliation: Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Nallely
authors.initials: N
authors.lastname: Cabrera
authors.affiliation: Departamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Mexico.
authors.forename: Aurora
authors.initials: A
authors.lastname: Sánchez-García
authors.affiliation: Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico.
authors.forename: Isabel
authors.initials: I
authors.lastname: Gómez
authors.affiliation: Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, SSA, Mexico.
authors.forename: Edgar
authors.initials: E
authors.lastname: Rangel-López
authors.affiliation: Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, SSA, Mexico. Electronic address: absada@yahoo.com.
authors.forename: Abel
authors.initials: A
authors.lastname: Santamaria
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000502:physiology; D003342:Corpus Striatum / Q000378:metabolism*; D008024:Ligands; D008297:Male; D016627:Oxidopamine / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism*
chemical_list: C000593874:Ager protein, rat; D008024:Ligands; D000067759:Receptor for Advanced Glycation End Products; D016627:Oxidopamine
keywords: 6-Hydroxydopamine; AGES; Binding constant; Docking; Neurotoxicity; RAGE expression