title: Supplementation of an Artificial Medium for the Parasitoid Exorista larvarum (Diptera: Tachnidae) With Hemolymph of Hermetia illucens (Diptera: Stratiomyidae) or Antheraea pernyi (Lepidoptera: Saturniidae).
abstract: The effect of supplementing hemolymph of the black soldier fly, Hermetia illucens (L.), or the Chinese oak silkworm, Antheraea pernyi (Guérin-Méneville), to a basic insect-free artificial medium for the tachinid Exorista larvarum (L.) was investigated. The supplementation (20% w/w) was based on the assumption that insect additives may optimize the media for this parasitoid. Egg hatch, pupal and adult yields, and sex ratio did not differ among the enriched and basic media. Preimaginal development was faster on both hemolymph-enriched media than on the basic medium. Despite the shorter development on the medium supplemented with H. illucens hemolymph than on the basic medium, on the two media puparium weights were comparable. The female flies reared on the medium enriched with H. illucens hemolymph did not lay more eggs, but the latter yielded significantly more puparia compared with the control females. Conversely, the medium enriched with A. pernyi hemolymph yielded lower female puparium weights than the basic medium and produced only one ovipositing female out of the five obtained female adults. These results indicate that the in vitro development of E. larvarum improved when the basic artificial medium was enriched with H. illucens hemolymph, whereas the supplementation with A. pernyi hemolymph negatively affected the quality of the in vitro-reared females.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Dindo
authors.forename: Jonas
authors.initials: J
authors.lastname: Vandicke
authors.forename: Elisa
authors.initials: E
authors.lastname: Marchetti
authors.forename: Thomas
authors.initials: T
authors.lastname: Spranghers
authors.forename: Jochem
authors.initials: J
authors.lastname: Bonte
authors.forename: Patrick
authors.initials: P
authors.lastname: De Clercq
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004175:Diptera / Q000254:growth & development*; D005260:Female; D006458:Hemolymph; D008297:Male