title: A label-free and portable graphene FET aptasensor for children blood lead detection.
abstract: Lead is a cumulative toxicant, which can induce severe health issues, especially in children's case due to their immature nervous system. While realizing large-scale monitoring of children blood lead remains challenging by utilizing traditional methods, it is highly desirable to search for alternative techniques or novel sensing materials. Here we report a label-free and portable aptasensor based on graphene field effect transistor (FET) for effective children blood lead detection. With standard solutions of different Pb(2+) concentrations, we obtained a dose-response curve and a detection limitation below 37.5 ng/L, which is three orders lower than the safe blood lead level (100 μg/L). The devices also showed excellent selectivity over other metal cations such as, Na(+), K(+), Mg(2+), and Ca(2+), suggesting the capability of working in a complex sample matrix. We further successfully demonstrated the detection of Pb(2+) ions in real blood samples from children by using our aptasensors, and explored their potential applications for quantification. Our results underscore such graphene FET aptasensors for future applications on fast detection of heavy metal ions for health monitoring and disease diagnostics.
authors.affiliation: National Laboratory of Solid State Microstructures, School of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.
authors.forename: Chenyu
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Cui
authors.affiliation: Jiangsu Engineering Technology Research Centre of Environmental Cleaning Materials, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Joint Laboratory of Atmospheric Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science &Technology, 219 Ningliu Road, Nanjing, Jiangsu 210044, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bi
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China.
authors.forename: Lena Q
authors.initials: LQ
authors.lastname: Ma
authors.affiliation: National Laboratory of Solid State Microstructures, School of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: National Laboratory of Solid State Microstructures, School of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: National Laboratory of Solid State Microstructures, School of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.
authors.forename: Baigeng
authors.initials: B
authors.lastname: Wang
authors.affiliation: National Laboratory of Solid State Microstructures, School of Physics, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing 210093, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Miao
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D001774:Blood Chemical Analysis; D002648:Child; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007854:Lead / Q000097:blood*
chemical_list: D052157:Aptamers, Nucleotide; D007854:Lead; D006108:Graphite