title: Effects of surface functional groups on proliferation and biofunction of Schwann cells.
abstract: Scaffolds in tissue engineering should be rationally designed to become an adhesion substrate friendly to cells. Schwann cells play an important role in nerve regeneration and repair. Previous studies have suggested that surface chemical groups have effect on many types of cells. However, there have hitherto been few reports on Schwann cells. In this study, we investigated cell adhesion, survival, proliferation, and neurotrophic actions of Schwann cells cultured on glass coverslips modified with different chemical groups, including methyl, carboxyl, amino, hydroxyl, mercapto, and sulfonic groups. Schwann cells on amino and carboxyl surfaces had higher attachment rate, presenting good morphology, high proliferation, and strong neurotrophic functions, while on methyl surfaces, few cells can survive, cells shrunk into round shape, exhibiting poor proliferation and weak neurotrophic functions. Growth of cells on other groups was between methyl and amino, carboxyl, and had little difference among them. Our data indicated that chemical groups can regulate behavior of Schwann cells, indicating a way to design new scaffolds for peripheral nerve regeneration.
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, P. R. China School of Chemistry and Chemical Engineering, Nantong University, Nantong, P. R. China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of cardiology, Affiliated Hospital of Nantong University, Nantong, P.R. China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, P. R. China Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, P. R. China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, P. R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: School of Medical, Nantong University, Nantong, P. R. China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, P. R. China Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, P. R. China yangym@ntu.edu.cn.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000586:Amination; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D008745:Methylation; D009416:Nerve Regeneration; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002264:Carboxylic Acids; D013438:Sulfhydryl Compounds; D013451:Sulfonic Acids
keywords: Schwann cells; adhesion; neurotrophic actions; proliferation; surface chemistry