title: Molecular studies of the immunological effects of the sevoflurane preconditioning in the liver and lung in a rat model of liver ischemia/reperfusion injury.
abstract: Sevoflurane has been shown to improve ischemia/reperfusion injury (IRI) through several mechanisms, including amelioration of inflammatory response. However, there haven't been any studies considering the potential role of the complement system in sevoflurane-mediated amelioration of ischemia/reperfusion injury. Our purpose was to investigate the molecular mechanisms involved in sevoflurane preconditioning in liver and lung injury induced by liver ischemia-reperfusion (LIR), giving emphasis to the immunological mechanisms. In order to do that, fifty male Wistar rats were randomly allocated in five groups (n=10 each): Animals in group LIR received ketamine and xylazine and were then subjected to ischemia of the right and median hepatic lobe for 45 min and reperfusion for 6h. Group SEVO/LIR received sevoflurane and then LIR was induced, as in group LIR. Animals in group SHAM/LIR were anesthetized with ketamine and xylazine and then laparotomy followed. Group SHAM/SEVO received sevoflurane for 30 min and then laparotomy followed. Finally, in group VEN, animals only received ketamine and xylazine. Our results showed that sevoflurane preconditioning significantly improved liver-biochemical tests (decreased Alanine transaminase (ALT), Alkaline phosphatase (ALP), Aspartate transaminase (AST) and Alkaline phosphatase (ALP) levels) and limited inflammatory cell infiltration in BALF. Additionally, compared with the LIR group, the reduction in plasma C3 was significantly reduced in the SEVO/LIR group. No significant differences were observed in histological examination in the liver and lung. Immunostaining of the liver for Intracellular Adhesion Molecule 1 (ICAM1) however, showed a decrease in ICAM1 levels in the SEVO/LIR group. In the lung, sevoflurane seemed to exert no effect in ICAM1 levels. Caspase 3 (CASP3) levels in the liver and the lung also appeared unaffected by sevoflurane preconditioning. In the SEVO/LIR group, ICAM1 mRNA expression was significantly reduced in the liver. No statistical significantly differences were observed in Complement component 3 (C3), Complement component 5 (C5) and Clusterin (CLU) mRNA levels in the liver or the lung tissue. Summarizing, sevoflurane preconditioning seems to ameliorate LIR-induced injury in the rats, mediated by mechanisms that include ICAM1 and complement C3 down regulation.
authors.affiliation: Department of Biology, School of Medicine, University of Patras, Greece. Electronic address: a_mikrou@yahoo.gr.
authors.forename: Angeliki
authors.initials: A
authors.lastname: Mikrou
authors.affiliation: Department of Anesthesiology, School of Medicine, University of Athens, Greece.
authors.forename: Konstantinos A
authors.initials: KA
authors.lastname: Kalimeris
authors.affiliation: Department of Anatomy, School of Medicine, University of Patras, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Lilis
authors.affiliation: Department of Anesthesiology, School of Medicine, University of Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Papoutsidakis
authors.affiliation: Department of Anesthesiology, School of Medicine, University of Athens, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Nastos
authors.affiliation: Department of Anatomy, School of Medicine, University of Patras, Greece.
authors.forename: Helen
authors.initials: H
authors.lastname: Papadaki
authors.affiliation: Department of Anesthesiology, School of Medicine, University of Athens, Greece.
authors.forename: Georgia G
authors.initials: GG
authors.lastname: Kostopanagiotou
authors.affiliation: Department of Biology, School of Medicine, University of Patras, Greece. Electronic address: zarkadis@med.upatras.gr.
authors.forename: Ioannis K
authors.initials: IK
authors.lastname: Zarkadis
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003167:Complement Activation / Q000187:drug effects; D003176:Complement C3 / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007511:Ischemia / Q000517:prevention & control*; D019194:Ischemic Preconditioning; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000187:drug effects; D008297:Male; D008738:Methyl Ethers / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D000077149:Sevoflurane
chemical_list: D003176:Complement C3; D008738:Methyl Ethers; D018799:Intercellular Adhesion Molecule-1; D000077149:Sevoflurane
keywords: C3; Complement; ICAM1; Ischemia/reperfusion; Sevoflurane