title: Poly-arginine conjugated triarylmethyl radical as intracellular spin label.
abstract: Stable triarylmethyl radicals are ideal spin labels used for biomedical electron paramagnetic resonance applications. Previously reported structures exhibit polar charged functions for water solubilization preventing them from crossing the cell membrane. We report the synthesis of a triarylmethyl radical conjugated to poly-arginine peptide allowing intracellular delivery of the paramagnetic label.
authors.affiliation: In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, United States; Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV 26506, United States.
authors.forename: Benoit
authors.initials: B
authors.lastname: Driesschaert
authors.affiliation: In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, United States; Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV 26506, United States.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Bobko
authors.affiliation: In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, United States; Department of Microbiology, Immunology & Cell Biology, West Virginia University School of Medicine, Morgantown, WV 26506, United States.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Eubank
authors.affiliation: Davis Heart & Lung Research Institute, The Ohio State University/Wexner Medical Center, 460 West 12th Avenue, BRT 0390, Columbus, OH 43210, United States.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Samouilov
authors.affiliation: In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV 26506, United States; Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV 26506, United States.
authors.forename: Valery V
authors.initials: VV
authors.lastname: Khramtsov
authors.affiliation: Davis Heart & Lung Research Institute, The Ohio State University/Wexner Medical Center, 460 West 12th Avenue, BRT 0390, Columbus, OH 43210, United States. Electronic address: jay.zweier@osumc.edu.
authors.forename: Jay L
authors.initials: JL
authors.lastname: Zweier
mesh_terms: D045744:Cell Line, Tumor; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D008697:Methane / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D013113:Spin Labels / Q000138:chemical synthesis*
chemical_list: D010455:Peptides; D013113:Spin Labels; C051224:methyl radical; C015462:polyarginine; D008697:Methane
keywords: Cyclic voltammetry; EPR; Poly-arginine; Radical; TAM