title: [Chromatographic Fingerprinting Study of Zhenyuan Granules Dry Extract by HPLC-DAD and HPLC-MS/MS].
abstract: OBJECTIVE
  To establish a novel, accurate and valid fingerprint method of Zhenyuan granules dry extract by using HPLC-DAD method, to study herbs belonging of fingerprint peaks and to identify some of the chromatographic peaks by HPLC-MS/MS analysis, for providing the basis for scientific evaluation of the quality.
  
  
  METHODS
  The sample solutions were analyzed by an Agilent SB C18 (250 mm x 4.6 mm, 5 µm) column, and gradiently eluted with acetonitrile (containing 0.1% formic acid) and aqueous phase (containing 0.1% formic acid) as the mobile phase. The flow rates were 1.2 mL/min (0~70 min) and 0.8 mL/min (70~150 min); the column temperature was 30 °C; and the detection wavelength was 254 nm.
  
  
  RESULTS
  40 peaks were selected as fingerprint peaks under the optimal chromatographic condition, and the similarity coefficients of 10 batches of Zhenyuan granules dry extract were all greater than 0.98. 27 peaks were tentatively identified with reference to literature data based on their mass spectrometry.
  
  
  CONCLUSIONS
  The chromatographic fingerprint of Zhenyuan granules is proved to be a reliable method for comprehensive quality control and assessment.
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Shan
authors.forename: Qing-jie
authors.initials: QJ
authors.lastname: Tan
authors.forename: Song-lin
authors.initials: SL
authors.lastname: Wang
authors.forename: Jian-lan
authors.initials: JL
authors.lastname: Jiang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts