title: Spatiotemporal dynamics of bacterial and archaeal communities in household biogas digesters from tropical and subtropical regions of Yunnan Province, China.
abstract: A combination of 16S rRNA gene PCR-based techniques and the determination of abiotic factors were used to study community composition, richness, and evenness and the correlation between biotic and abiotic factors in 19 household biogas digesters in tropical and subtropical regions of Yunnan Province, China. The results revealed that both bacterial and archaeal community composition differed between regions and archaeal community composition was more affected by season than bacterial; regardless of sampling location, the dominant bacterial phyla included Chloroflexi, Bacteroidetes, Firmicutes, and Proteobacteria, and the most dominant archaeal phylum was Euryarchaeota; in digesters from both regions, Chloroflexi as the first or second most dominant bacteria accounted for 21.50-26.10 % of bacterial library sequences, and the phylum Crenarchaeota as the second most dominant archaea accounted for 17.65-19.77 % of archaeal library sequences; the species Methanosaeta concilii as the most dominant archaeal species accounted for 67.80-72.80 % of the sequences. This study found that most of the abundant microbial communities in 19 biogas digesters are similar, and this result will provide enlightenment for finding the universal nature in rural biogas digesters at tropical and subtropical regions in China.
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
authors.forename: Guangliang
authors.initials: G
authors.lastname: Tian
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
authors.forename: Qiumin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Dong
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
authors.forename: Yingjuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yin
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China.
authors.forename: Xingling
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China. xlcuiynu@aliyun.com.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Cui
authors.affiliation: Yunnan Research Center of Biogas Technology and Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China. wootichang@163.com.|Engineering and Research Center of Sustainable Development and Utilization of Bioenergy, Ministry of Education, Yunnan Normal University, Kunming, 650500, People's Republic of China. wootichang@163.com.|Yunnan Key Laboratory of Rural Energy Engineering, Yunnan Normal University, Kunming, 650500, People's Republic of China. wootichang@163.com.
authors.forename: Wudi
authors.initials: W
authors.lastname: Zhang
mesh_terms: D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis; D019149:Bioreactors / Q000382:microbiology*; D064307:Microbiota / Q000502:physiology*; D019642:RNA, Archaeal / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D056804:Biofuels; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Abiotic factors; Clone library; Household biogas digester; Microbial community; Tropical region