title: Epigenetic Silencing of the Key Antioxidant Enzyme Catalase in Karyotypically Abnormal Human Pluripotent Stem Cells.
abstract: Epigenomic regulation is likely to be important in the maintenance of genomic integrity of human pluripotent stem cells, however, the mechanisms are unknown. We explored the epigenomes and transcriptomes of human pluripotent stem cells before and after spontaneous transformation to abnormal karyotypes and in correlation to cancer cells. Our results reveal epigenetic silencing of Catalase, a key regulator of oxidative stress and DNA damage control in abnormal cells. Our findings provide novel insight into the mechanisms associated with spontaneous transformation of human pluripotent stem cells towards malignant fate. The same mechanisms may control the genomic stability of cells in somatic tissues.
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Mikko
authors.initials: M
authors.lastname: Konki
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Kalyan
authors.initials: K
authors.lastname: Pasumarthy
authors.affiliation: Department of Information and Computer Science, Aalto University School of Science, Helsinki, Finland.
authors.forename: Maia
authors.initials: M
authors.lastname: Malonzo
authors.affiliation: Department of Medical Biochemistry and Genetics, University of Turku, and Department of Medicine, Division of Endocrinology, Turku University Hospital, Turku, Finland.
authors.forename: Annele
authors.initials: A
authors.lastname: Sainio
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.|Department of Medicine, Division of Medical Genetics and Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Valensisi
authors.affiliation: Department of Pathology, Turku University Hospital, University of Turku, Turku, Finland.
authors.forename: Mirva
authors.initials: M
authors.lastname: Söderström
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Maheswara Reddy
authors.initials: MR
authors.lastname: Emani
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Aki
authors.initials: A
authors.lastname: Stubb
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Elisa
authors.initials: E
authors.lastname: Närvä
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Bishwa
authors.initials: B
authors.lastname: Ghimire
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Asta
authors.initials: A
authors.lastname: Laiho
authors.affiliation: Department of Medical Biochemistry and Genetics, University of Turku, and Department of Medicine, Division of Endocrinology, Turku University Hospital, Turku, Finland.
authors.forename: Hannu
authors.initials: H
authors.lastname: Järveläinen
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
authors.forename: Riitta
authors.initials: R
authors.lastname: Lahesmaa
authors.affiliation: Department of Information and Computer Science, Aalto University School of Science, Helsinki, Finland.
authors.forename: Harri
authors.initials: H
authors.lastname: Lähdesmäki
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.|Department of Medicine, Division of Medical Genetics and Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.
authors.forename: R David
authors.initials: RD
authors.lastname: Hawkins
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.|Centre for Stem Cell Biology, the Department of Biomedical Science, University of Sheffield, Sheffield, UK.
authors.forename: Riikka J
authors.initials: RJ
authors.lastname: Lund
mesh_terms: D059786:Abnormal Karyotype; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D020868:Gene Silencing; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D039904:Pluripotent Stem Cells / Q000201:enzymology / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D002374:Catalase