title: Immunogenicity of parenterally delivered plant-derived small and medium surface antigens of hepatitis B virus.
abstract: Intramuscularly delivered plant-derived M-HBsAg was compared to S-HBsAg, and as a result elicited specific anti-preS2 antibodies and significantly higher titre of anti-HBs antibodies, together with IgG isotype profile indicating some Th1 polarisation, apart from the main Th2 response. HBV prevalence is still threatening, regardless of prevention programmes using vaccines containing S-HBsAg, supplemented by third-generation vaccines, comprising also M- and L-HBsAg. Plant expression systems offer a cost-effective production option of the antigens. Plant-derived S- and M-HBsAg, intramuscularly delivered to mice, elicited anti-HBs antibodies several times higher than high responsiveness threshold titre. M-HBsAg induced stronger response of anti-HBs and also specific anti-preS2 antibodies. IgG isotype profiles indicated mainly Th2 response, yet Th1 polarisation was also pointed out, in some larger extent for M-HBsAg. These results correspond to research on CHO-derived M-HBsAg vs. commercial vaccines based on S-HBsAg and support potency of plant-derived antigens as alternative injection vaccines.
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznan, Poland.
authors.forename: Olga
authors.initials: O
authors.lastname: Fedorowicz-Strońska
authors.affiliation: Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland.
authors.forename: Józef
authors.initials: J
authors.lastname: Kapusta
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznan, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Czyż
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznan, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kaczmarek
authors.affiliation: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznan, Poland. tpni@igr.poznan.pl.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Pniewski
mesh_terms: D000818:Animals; D006509:Hepatitis B / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage*; D006515:Hepatitis B virus / Q000276:immunology*; D007273:Injections, Intramuscular; D051379:Mice
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines
keywords: Anti-HBs antibodies; Anti-preS2 antibodies; IgG isotypes; M-HBsAg; Plant-derived vaccines; S-HBsAg