title: Next generation sequencing approach for detecting 491 fusion genes from human cancer.
abstract: Next-generation DNA sequencing (NGS) of the genomes of cancer cells is contributing to new discoveries that illuminate the mechanisms of tumorigenesis. To this end, the International Cancer Genome Consortium and The Cancer Genome Atlas are investigating novel alterations of genes that will define the pathways and mechanisms of the development and growth of cancers. These efforts contribute to the development of innovative pharmaceuticals as well as to the introduction of genome sequencing as a component of personalized medicine. In particular, chromosomal translocations that fuse coding sequences serve as important pharmaceutical targets and diagnostic markers given their association with tumorigenesis. Although increasing numbers of fusion genes are being discovered using NGS, the methodology used to identify such fusion genes is complicated, expensive, and requires relatively large samples. Here, to address these problems, we describe the design and development of a panel of 491 fusion genes that performed well in the analysis of cultured human cancer cell lines and 600 clinical tumor specimens.
authors.affiliation: Cancer Diagnostics Research Division, Shizuoka Cancer Center Research Institute.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Urakami
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shimoda
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagashima
authors.forename: Masakuni
authors.initials: M
authors.lastname: Serizawa
authors.forename: Tomoe
authors.initials: T
authors.lastname: Tanabe
authors.forename: Junko
authors.initials: J
authors.lastname: Saito
authors.forename: Tamiko
authors.initials: T
authors.lastname: Usui
authors.forename: Yuko
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Akane
authors.initials: A
authors.lastname: Naruoka
authors.forename: Sumiko
authors.initials: S
authors.lastname: Ohnami
authors.forename: Shumpei
authors.initials: S
authors.lastname: Ohnami
authors.forename: Tohru
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kusuhara
authors.forename: Ken
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
chemical_list: D015514:Oncogene Proteins, Fusion