title: A novel self-enhanced electrochemiluminescence immunosensor based on hollow Ru-SiO2@PEI nanoparticles for NSE analysis.
abstract: Poly(ethylenimine) (PEI) and Ru(bpy)3(2+)-doped silica (Ru-SiO2) nanoparticles were simply mixed together to prepare a novel self-enhanced electrochemiluminescence (ECL) composite of Ru-SiO2@PEI. The hollow Ru-SiO2@PEI nanoparticles were used to build an ECL immunosensor for the analysis of neuron specific enolase (NSE). PEI not only assembled on the surface of Ru-SiO2 nanoparticles through the electrostatic interaction to act as co-reactant for Ru(bpy)3(2+) ECL, but also provided alkaline condition to etch the Ru-SiO2 nanoparticles to form the hollow Ru-SiO2@PEI nanoparticles with porous shell. The unique structure of the Ru-SiO2@PEI nanoparticles loaded both a large amount of Ru(bpy)3(2+) and its co-reactant PEI at the same time, which shortened the electron-transfer distance, thereby greatly enhanced the luminous efficiency and amplified the ECL signal. The developed immunosensor showed a wide linear range from 1.0 × 10(-11) to 1.0 × 10(-5) mg mL(-1) with a low detection limit of 1.0 × 10(-11) mg mL(-1) for NSE. When the immunosensor was used for the determination of NSE in clinical human serum, the results were comparable with those obtained by using enzyme-linked immunosorbent assay (ELISA) method. The proposed method provides a promising alternative for NSE analysis in clinical samples.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.|University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.|National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Tianyan
authors.initials: T
authors.lastname: You
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004563:Electrochemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006046:Gold / Q000737:chemistry; D006801:Humans; D007097:Imines / Q000737:chemistry; D007118:Immunoassay; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D011095:Polyethylenes / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D007097:Imines; D011095:Polyethylenes; C505405:poly(ethylene imine); D006046:Gold; D012822:Silicon Dioxide; D010751:Phosphopyruvate Hydratase