title: Chemisorption Mechanism of DNA on Mg/Fe Layered Double Hydroxide Nanoparticles: Insights into Engineering Effective SiRNA Delivery Systems.
abstract: Layered double hydroxide nanoparticles (LDH NPs) have attracted interest as an effective gene delivery vehicle in biomedicine. Recent advances in clinic trials have demonstrated the efficacy of Mg/Fe LDHs for hyperphosphatemia treatment, but their feasibility for gene delivery has not been systematically evaluated. As a starting point, we aimed to study the interaction between oligo-DNA and Mg/Fe LDH NPs. Our investigation revealed the chemisorption mechanism of DNA on Mg/Fe LDH surfaces, wherein the phosphate backbone of the DNA polymer coordinates with the metal cations of the LDH lattice via the ligand-exchange process. This mechanistic insight may facilitate future gene delivery applications using Mg/Fe LDH NPs.
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University , Shanghai 201306, People's Republic of China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Lu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Shan
authors.forename: Kori
authors.initials: K
authors.lastname: Andrea
authors.forename: Bruce
authors.initials: B
authors.lastname: MacDonald
authors.affiliation: Department of Physics, Acadia University , Wolfville, Nova Scotia B4P 2R6, Canada.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Beale
authors.affiliation: Department of Biology, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Dennis E
authors.initials: DE
authors.lastname: Curry
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University , Hangzhou, Zhejiang 310018, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Jiangsu Marine Resources Development Research Institute , Lianyungang, Jiangsu 222001, People's Republic of China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Wang
authors.forename: Ken D
authors.initials: KD
authors.lastname: Oakes
authors.affiliation: Department of Physics, Acadia University , Wolfville, Nova Scotia B4P 2R6, Canada.
authors.forename: Craig
authors.initials: C
authors.lastname: Bennett
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University , Shanghai 201306, People's Republic of China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Wu
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D005290:Ferric Compounds / Q000737:chemistry; D018014:Gene Transfer Techniques; D006878:Hydroxides / Q000737:chemistry*; D017616:Magnesium Compounds; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; D006878:Hydroxides; D017616:Magnesium Compounds; D009838:Oligodeoxyribonucleotides; D034741:RNA, Small Interfering