title: A novel DNA biosensor integrated with Polypyrrole/streptavidin and Au-PAMAM-CP bionanocomposite probes to detect the rs4839469 locus of the vangl1 gene for dysontogenesis prediction.
abstract: The single nucleotide polymorphism (SNP) of the vangl1 gene is highly correlated with Neural Tube Defects (NTDs), a group of severe congenital malformations. It is hindered by the lack of a quantitative detection method. We first propose the use of a DNA biosensor to detect the missense single nucleotide polymorphism (rs4839469 c.346G>A p.Ala116Thr) of the vangl1 gene in this work. Polypyrrole (PPy) and streptavidin were integrated to modify a gold electrode. We took advantage of the PPy's good biocompatibility and excellent conductivity. To further accelerate the electron transfer process at the electrode surface, polyamidoamine dendrimer-encapsulated gold nanoparticles (Au-PAMAM) were used, because Au-PAMAM possess a large number of amino groups to load capture probes (CP). Using the biotin-streptavidin system, the Au-PAMAM-CP bionanocomposite probe, which can detect the target DNA, was conjugated to the electrode surface. Under optimal conditions, the DNA biosensor exhibited a wide linear range of 0.1-100 nM with a low detection limit of 0.033 nM (S/N=3). The results suggest that this approach has the potential to be used in clinical research.
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Qingying
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Rufei
authors.initials: R
authors.lastname: Gao
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Chunyong
authors.initials: C
authors.lastname: Xia
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Yuan
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China.
authors.forename: Qiutong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: School of Public Health and Management, Institute of Life Science, Chongqing Medical University, Box 197, No.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, PR China. Electronic address: hejunlin_11@aliyun.com.
authors.forename: Junlin
authors.initials: J
authors.lastname: He
mesh_terms: D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification*; D053761:Nanocomposites / Q000737:chemistry; D009436:Neural Tube Defects / Q000175:diagnosis* / Q000235:genetics; D011108:Polymers / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011758:Pyrroles / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D011108:Polymers; D011758:Pyrroles; C488287:VANGL1 protein, human; C067635:polypyrrole; D006046:Gold; D004247:DNA; D019809:Streptavidin
keywords: Au-PAMAM; Capture probe; DNA biosensor; PPy/ streptavidin