title: The human DNA ends proteome uncovers an unexpected entanglement of functional pathways.
abstract: DNA ends get exposed in cells upon either normal or dysfunctional cellular processes or molecular events. Telomeres need to be protected by the shelterin complex to avoid junctions occurring between chromosomes while failing topoisomerases or clustered DNA damage processing may produce double-strand breaks, thus requiring swift repair to avoid cell death. The rigorous study of the great many proteins involved in the maintenance of DNA integrity is a challenging task because of the innumerous unspecific electrostatic and/or hydrophobic DNA-protein interactions that arise due to the chemical nature of DNA. We devised a technique that discriminates the proteins recruited specifically at DNA ends from those that bind to DNA because of a generic affinity for the double helix. Our study shows that the DNA ends proteome comprises proteins of an unexpectedly wide functional spectrum, ranging from DNA repair to ribosome biogenesis and cytoskeleton, including novel proteins of undocumented function. A global mapping of the identified proteome on published DNA repair protein networks demonstrated the excellent specificity and functional coverage of our purification technique. Finally, the native nucleoproteic complexes that assembled specifically onto DNA ends were shown to be endowed with a highly efficient DNA repair activity.
authors.affiliation: Laboratoire de chimie physique, UMR CNRS 8000, University of Paris-Sud, F-91400 Orsay, France.
authors.forename: Vivien
authors.initials: V
authors.lastname: Berthelot
authors.affiliation: Structure et Instabilité des Génomes, INSERM U1154, UMR CNRS/MNHN 7196, F-75005 Paris, France.
authors.forename: Gildas
authors.initials: G
authors.lastname: Mouta-Cardoso
authors.affiliation: Structure et Instabilité des Génomes, INSERM U1154, UMR CNRS/MNHN 7196, F-75005 Paris, France.
authors.forename: Nadia
authors.initials: N
authors.lastname: Hégarat
authors.affiliation: Plateforme de spectrométrie de masse 3P5, Institut Cochin, F-75014 Paris, France.
authors.forename: François
authors.initials: F
authors.lastname: Guillonneau
authors.affiliation: Inserm and Sorbonne Universities, UPMC, UMR_S 938, Research Center Saint-Antoine, F-75012 Paris, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: François
authors.affiliation: Structure et Instabilité des Génomes, INSERM U1154, UMR CNRS/MNHN 7196, F-75005 Paris, France.
authors.forename: Carine
authors.initials: C
authors.lastname: Giovannangeli
authors.affiliation: Structure et Instabilité des Génomes, INSERM U1154, UMR CNRS/MNHN 7196, F-75005 Paris, France praseuth@mnhn.fr.
authors.forename: Danièle
authors.initials: D
authors.lastname: Praseuth
authors.affiliation: Laboratoire de chimie physique, UMR CNRS 8000, University of Paris-Sud, F-91400 Orsay, France filippo.rusconi@u-psud.fr.
authors.forename: Filippo
authors.initials: F
authors.lastname: Rusconi
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009698:Nucleoproteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009698:Nucleoproteins; D020543:Proteome; D004247:DNA