title: Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear.
abstract: BACKGROUND
  Long-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic field attracting forces generated by CI electrodes to bind BDNF-secreting cells that are labelled with magnetic beads (MB) onto the electrode surfaces. Thus, the effect of MB-labelling on cell viability and BDNF production were investigated.
  
  
  METHODS
  Murine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB.
  
  
  RESULTS
  Atomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release.
  
  
  CONCLUSIONS
  Our data demonstrate a novel approach for mediating enhanced long-term adhesion of BDNF-secreting fibroblasts on model electrode surfaces for cell-based drug delivery applications in vitro and in vivo. This therapeutic strategy, once transferred to cells suitable for clinical application, may allow the biological modifications of CI surfaces with cells releasing neurotrophic or other factors of interest.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.|Cluster of Excellence "Hearing4All", Hannover, Germany.
authors.forename: Pooyan
authors.initials: P
authors.lastname: Aliuos
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.|Cluster of Excellence "Hearing4All", Hannover, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Schulze
authors.affiliation: Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Schomaker
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Reuter
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Stefan R O
authors.initials: SR
authors.lastname: Stolle
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Darja
authors.initials: D
authors.lastname: Werner
authors.affiliation: Cluster of Excellence "Hearing4All", Hannover, Germany.|Laser Zentrum Hannover e.V., Hannover, Germany.
authors.forename: Tammo
authors.initials: T
authors.lastname: Ripken
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.|Cluster of Excellence "Hearing4All", Hannover, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lenarz
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.|Cluster of Excellence "Hearing4All", Hannover, Germany.
authors.forename: Athanasia
authors.initials: A
authors.lastname: Warnecke
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage* / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects*; D003054:Cochlear Implants; D016503:Drug Delivery Systems; D004343:Drug Implants; D007758:Ear, Inner / Q000187:drug effects*; D008280:Magnetics; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004343:Drug Implants