title: High-Throughput Measurement of Binding Kinetics by mRNA Display and Next-Generation Sequencing.
abstract: There is great demand for high-throughput methods to characterize ligand affinity. By combining mRNA display with next-generation sequencing, we determined the kinetic on- and off-rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement.
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA, 90089, USA.
authors.forename: Farzad
authors.initials: F
authors.lastname: Jalali-Yazdi
authors.affiliation: Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, USA.
authors.forename: Lan Huong
authors.initials: LH
authors.lastname: Lai
authors.affiliation: Department of Chemistry, University of Southern California, Los Angeles, CA, 90089, USA.
authors.forename: Terry T
authors.initials: TT
authors.lastname: Takahashi
authors.affiliation: Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA, 90089, USA. richrob@usc.edu.|Department of Molecular Computational Biology, USC Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, 90089, USA. richrob@usc.edu.
authors.forename: Richard W
authors.identifier: http://orcid.org/0000-0002-8587-5097
authors.initials: RW
authors.lastname: Roberts
mesh_terms: D059014:High-Throughput Nucleotide Sequencing; D007700:Kinetics; D008024:Ligands; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D008024:Ligands; D012333:RNA, Messenger
keywords: affinity measurements; dissociation constants; high-throughput screenings; kinetics; protein-protein interactions