title: Effect of different agents with potential antibiofilm activity on antimicrobial susceptibility of biofilms formed by Staphylococcus spp. isolated from implant-related infections.
abstract: An in vitro study aimed to evaluate the effect of N-acetyl cysteine (NAC) or sub-ICs of erythromycin on antimicrobial susceptibility of staphylococcal biofilms was performed. Staphylococcus aureus and Staphylococcus epidermidis strains were isolated from patients with prosthetic joint infections using a previously published sonication procedure. Conventional susceptibility studies were performed using microdilution according to the CLSI procedures. Biofilm susceptibility was performed using the Calgary methodology. The addition of NAC showed no effect with the S. aureus strains, and a strain-dependent effect with the S. epidermidis strains. No effect was detected with erythromycin for almost all tested strains.
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Diana
authors.initials: D
authors.lastname: Molina-Manso
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Del-Prado
authors.affiliation: Department of Orthopaedic Surgery, IdIPaz, Hospital Universitario La Paz, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Gómez-Barrena
authors.affiliation: Department of Orthopaedic Surgery, Hospital Universitario La Princesa, Madrid, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Cordero-Ampuero
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernandez-Roblas
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Jaime
authors.identifier: 0000-0002-8971-3167
authors.initials: J
authors.lastname: Esteban
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D004917:Erythromycin / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D016459:Prosthesis-Related Infections / Q000188:drug therapy* / Q000382:microbiology; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004917:Erythromycin; D000111:Acetylcysteine