title: The Endo-β-1,4-Glucanase of Bacillus amyloliquefaciens Is Required for Optimum Endophytic Colonization of Plants.
abstract: The eglS gene in Bacillus amyloliquefaciens encodes an endo-β-1,4-glucanase that belongs to glycosyl hydrolase family 5. In this study, a disruption mutant of gene eglS was constructed to examine its role in bacterial adaptation in plants. The mutant TB2k, eglS gene inactivated bacterial strain, was remarkably impaired in extracellular cellulase activity. When inoculated on Brassica campestris, the TB2k population was reduced by more than 60% compared with the wild-type strain in the root, stem, and leaf tissues. Overexpression of eglS in the wild-type strain increased the bacteria population in the plant tissues. Further studies revealed that the transcription level of eglS was correlated with bacterial population. These data demonstrate that endo-β-1,4-glucanase of B. amyloliquefaciens is required for its optimal endophytic colonization.
authors.affiliation: College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P.R. China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang, Henan 471023, P.R. China.
authors.forename: Ruixian
authors.initials: R
authors.lastname: Yang
authors.affiliation: The Crop Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, P.R. China.
authors.forename: Sixin
authors.initials: S
authors.lastname: Qiu
authors.affiliation: College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P.R. China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Cai
authors.affiliation: College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P.R. China.
authors.forename: Huasong
authors.initials: H
authors.lastname: Zou
authors.affiliation: College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, P.R. China.
authors.forename: Fangping
authors.initials: F
authors.lastname: Hu
mesh_terms: D000069977:Bacillus amyloliquefaciens / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001937:Brassica / Q000382:microbiology*; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D060026:Endophytes / Q000201:enzymology*; D004789:Enzyme Activation; D005798:Genes, Bacterial; D016296:Mutagenesis; D009154:Mutation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; D002480:Cellulase
keywords: Bacillus amyloliquefaciens; colonization; endo-β-1,4-glucanase; endophytic