title: Anti-Microbial Dendrimers against Multidrug-Resistant P. aeruginosa Enhance the Angiogenic Effect of Biological Burn-wound Bandages.
abstract: Multi-drug resistant Pseudomonas aeruginosa has increased progressively and impedes further regression in mortality in burn patients. Such wound infections serve as bacterial reservoir for nosocomial infections and are associated with significant morbidity and costs. Anti-microbial polycationic dendrimers G3KL and G3RL, able to kill multi-drug resistant P. aeruginosa, have been previously developed. The combination of these dendrimers with a class of biological bandages made of progenitor skin cells, which secrete growth factors, could positively impact wound-healing processes. However, polycations are known to be used as anti-angiogenic agents for tumor suppression. Since, neovascularization is pivotal in the healing of deep burn-wounds, the use of anti-microbial dendrimers may thus hinder the healing processes. Surprisingly, we have seen in this study that G3KL and G3RL dendrimers can have angiogenic effects. Moreover, we have shown that a dendrimer concentration ranging between 50 and 100 μg/mL in combination with the biological bandages can suppress bacterial growth without altering cell viability up to 5 days. These results show that antimicrobial dendrimers can be used in combination with biological bandages and could potentially improve the healing process with an enhanced angiogenesis.
authors.affiliation: Laboratory of Biomechanical Orthopedics, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland.
authors.forename: Philippe
authors.initials: P
authors.lastname: Abdel-Sayed
authors.forename: Ariane
authors.initials: A
authors.lastname: Kaeppeli
authors.affiliation: Laboratory of Biomechanical Orthopedics, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland.
authors.forename: Ariane
authors.initials: A
authors.lastname: Kaeppli
authors.affiliation: Department of Chemistry and Biochemistry University of Bern, Switzerland.
authors.forename: Thissa
authors.initials: T
authors.lastname: Siriwardena
authors.affiliation: Department of Chemistry and Biochemistry University of Bern, Switzerland.
authors.forename: Tamis
authors.initials: T
authors.lastname: Darbre
authors.affiliation: Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, Switzerland.
authors.forename: Karl
authors.initials: K
authors.lastname: Perron
authors.affiliation: Regenerative Therapy Unit, University Hospital of Lausanne, Switzerland.
authors.forename: Paris
authors.initials: P
authors.lastname: Jafari
authors.affiliation: Department of Chemistry and Biochemistry University of Bern, Switzerland.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Reymond
authors.affiliation: Laboratory of Biomechanical Orthopedics, Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Switzerland.
authors.forename: Dominique P
authors.initials: DP
authors.lastname: Pioletti
authors.affiliation: Regenerative Therapy Unit, University Hospital of Lausanne, Switzerland.
authors.forename: Lee Ann
authors.initials: LA
authors.lastname: Applegate
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001458:Bandages; D002056:Burns / Q000150:complications*; D002470:Cell Survival / Q000187:drug effects; D050091:Dendrimers; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014945:Wound Healing; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D043925:Angiogenesis Inducing Agents; D000890:Anti-Infective Agents; D050091:Dendrimers