title: AKT-p53 axis protect cancer cells from autophagic cell death during nutrition deprivation.
abstract: An altered metabolism supports growth of tumor. AKT, a major signal integrator plays a key role in cell metabolism. We have shown that nutritional deprivation activates AKT as observed by increased phosphorylation of both Thr308 and Ser473. Pharmacological inhibition or silencing of AKT by siRNA affects cell viability during starvation. The tumor suppressor, p53 is also observed to be elevated during nutritional deprivation due to AKT. Silencing of AKT and p53 enhanced autophagy as evidenced by increased acidic vesicular organelles and LC3B II levels, suggesting AKT-p53 to play a significant role in cell survival through regulating autophagy during nutritional deprivation.
authors.affiliation: Centre for Biotechnology, Department of Biotechnology, Anna University, Chennai, India.
authors.forename: S
authors.initials: S
authors.lastname: Sudhagar
authors.affiliation: Centre for Biotechnology, Department of Biotechnology, Anna University, Chennai, India.
authors.forename: S
authors.initials: S
authors.lastname: Sathya
authors.affiliation: Centre for Biotechnology, Department of Biotechnology, Anna University, Chennai, India.
authors.forename: G
authors.initials: G
authors.lastname: Gokulapriya
authors.affiliation: Centre for Biotechnology, Department of Biotechnology, Anna University, Chennai, India; Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, India. Electronic address: lakshmibs@annauniv.edu.
authors.forename: B S
authors.initials: BS
authors.lastname: Lakshmi
mesh_terms: D001343:Autophagy / Q000502:physiology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D055550:Protein Stability; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D005973:Glutamine; D051057:Proto-Oncogene Proteins c-akt; D005947:Glucose
keywords: AKT; Autophagy; Breast cancer; Nutrition deprivation; p53