title: 18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters.
abstract: Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collected surface water samples from the nearshore waters of four Chicago area beaches: Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach. Four biological replicates were collected for each sampling date and location, resulting in 112 samples. Eighty-nine of these samples were surveyed through targeted sequencing of the V7 and V8 regions of the 18S rDNA gene. Both technical and biological replicates were sequenced and are included in this dataset. Raw sequence data is available via NCBI's SRA database (BioProject PRJNA294919).
authors.affiliation: The Woodhall School, 58 Harrison Lane, Behlehem, CT 06751, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Searle
authors.affiliation: Department of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Sible
authors.affiliation: Department of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA.
authors.forename: Alexandria
authors.initials: A
authors.lastname: Cooper
authors.affiliation: Department of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA; Bioinformatics Program, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA; Department of Computer Science, Loyola University Chicago, 820 N Michigan Ave, Chicago, IL 60611, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Putonti
keywords: Freshwater; Lake Michigan; Metagenomics; Microeukaryotic community