title: Cytotoxic sesquiterpenoids from Ligularia pleurocaulis.
abstract: The phytochemical investigation of whole plants of Ligularia pleurocaulis led to isolation of five sesquiterpenoids, including an eremophilenolide dimer named biligupleurolide, along with eight known sesquiterpenoids. Their structures were determined by spectroscopic methods including 2D NMR techniques, and the structures of biligupleurolide and ligupleurolide were confirmed by X-ray diffraction analysis. All of the isolated compounds were tested for their cytotoxic activity against three cancer cell lines (HepG2, A549 and MCF-7), and biligupleurolide showed moderate inhibitory activities, with IC50 values of 5.2, 5.6 and 8.4 μM against MCF-7, HepG2 and A549, respectively.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu 610054, China. Electronic address: gbxie@uestc.edu.cn.
authors.forename: Guang-Bo
authors.initials: GB
authors.lastname: Xie
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu 610054, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Xie
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu 610054, China.
authors.forename: Yang-Zhirong
authors.initials: YZ
authors.lastname: Hu
authors.affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Zhu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D019659:Asteraceae / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012717:Sesquiterpenes; C000611151:biligupleurolide
keywords: Biligupleurolide; Compositae; Cu Kα X-ray crystallography; Cytotoxicity; Ligularia pleurocaulis; Sesquiterpenoid