title: Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide).
abstract: Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. sylvie.skalickova@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. sylvie.skalickova@gmail.com.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Skalickova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. lukasnejdl@gmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. lukasnejdl@gmail.com.
authors.forename: Lukas
authors.initials: L
authors.lastname: Nejdl
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. george.kudr@centrum.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. george.kudr@centrum.cz.
authors.forename: Jiri
authors.initials: J
authors.lastname: Kudr
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. brano.ruttkay@seznam.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. brano.ruttkay@seznam.cz.
authors.forename: Branislav
authors.initials: B
authors.lastname: Ruttkay-Nedecky
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. anuskajj@hotmail.com.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. anuskajj@hotmail.com.
authors.forename: Ana Maria Jimenez
authors.initials: AM
authors.lastname: Jimenez
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. paulko@centrum.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. paulko@centrum.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopel
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. mkremplova@volny.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. mkremplova@volny.cz.
authors.forename: Monika
authors.initials: M
authors.lastname: Kremplova
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. michal.masarik@mail.muni.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. michal.masarik@mail.muni.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Masarik
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, CZ-12840 Prague, Czech Republic. marie.stiborova@natur.cuni.cz.
authors.forename: Marie
authors.initials: M
authors.lastname: Stiborova
authors.affiliation: Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University, and University Hospital, Motol V Uvalu 84, CZ-15006 Prague, Czech Republic. Tomas.Eckschlager@fnmotol.cz.
authors.forename: Tomas
authors.initials: T
authors.lastname: Eckschlager
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. vojtech.adam@mendelu.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. vojtech.adam@mendelu.cz.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. kizek@sci.muni.cz.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. kizek@sci.muni.cz.
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D016373:DNA, Antisense / Q000737:chemistry / Q000627:therapeutic use*; D004317:Doxorubicin / Q000737:chemistry / Q000627:therapeutic use; D016503:Drug Delivery Systems; D004611:Ellipticines / Q000737:chemistry / Q000627:therapeutic use; D005047:Etoposide / Q000737:chemistry / Q000627:therapeutic use; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000627:therapeutic use; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D000071447:N-Myc Proto-Oncogene Protein / Q000037:antagonists & inhibitors / Q000235:genetics; D009369:Neoplasms / Q000188:drug therapy*
chemical_list: D016373:DNA, Antisense; D004611:Ellipticines; D008081:Liposomes; D058185:Magnetite Nanoparticles; D000071447:N-Myc Proto-Oncogene Protein; D005047:Etoposide; D006046:Gold; D004317:Doxorubicin
keywords: N-myc; doxorubicin; ellipticine; etoposide; gold nanoparticles; liposome