title: Architecture of the light-harvesting apparatus of the eustigmatophyte alga Nannochloropsis oceanica.
abstract: We present proteomic, spectroscopic, and phylogenetic analysis of light-harvesting protein (Lhc) function in oleaginous Nannochloropsis oceanica (Eustigmatophyta, Stramenopila). N. oceanica utilizes Lhcs of multiple classes: Lhcr-type proteins (related to red algae LHCI), Lhcv (VCP) proteins (violaxanthin-containing Lhcs related to Lhcf/FCP proteins of diatoms), Lhcx proteins (related to Lhcx/LhcSR of diatoms and green algae), and Lhc proteins related to Red-CLH of Chromera velia. Altogether, 17 Lhc-type proteins of the 21 known from genomic data were found in our proteomic analyses. Besides Lhcr-type antennas, a RedCAP protein and a member of the Lhcx protein subfamily were found in association with Photosystem I. The free antenna fraction is formed by trimers of a mixture of Lhcs of varied origins (Lhcv, Lhcr, Lhcx, and relatives of Red-CLH). Despite possessing several proteins of the Red-CLH-type Lhc clade, N. oceanica is not capable of chromatic adaptation under the same conditions as the diatom Phaeodactylum tricornutum or C. velia. In addition, a naming scheme of Nannochloropsis Lhcs is proposed to facilitate further work.
authors.affiliation: Biology Centre CAS, Branišovská 31, 370 05, České Budějovice, Czech Republic. litvin@umbr.cas.cz.|Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, České Budějovice, Czech Republic. litvin@umbr.cas.cz.
authors.forename: Radek
authors.identifier: http://orcid.org/0000-0001-5272-9029
authors.initials: R
authors.lastname: Litvín
authors.affiliation: Biology Centre CAS, Branišovská 31, 370 05, České Budějovice, Czech Republic.|Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, České Budějovice, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Bína
authors.affiliation: Biology Centre CAS, Branišovská 31, 370 05, České Budějovice, Czech Republic.|Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, České Budějovice, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Herbstová
authors.affiliation: Biology Centre CAS, Branišovská 31, 370 05, České Budějovice, Czech Republic.|Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, České Budějovice, Czech Republic.
authors.forename: Zdenko
authors.initials: Z
authors.lastname: Gardian
mesh_terms: D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045331:Photosystem I Protein Complex / Q000378:metabolism; D010802:Phylogeny; D013056:Spectrophotometry, Ultraviolet; D058009:Stramenopiles / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex
keywords: Light harvesting; Thylakoid membrane; Vaucheriaxanthin; Violaxanthin–chlorophyll protein