title: Analysis of Mouse Growth Plate Development.
abstract: To investigate skeletal development, pathophysiological mechanisms of cartilage and bone disease, and eventually assess innovative treatments, the mouse is a very important resource. During embryonic development, mesenchymal condensations are formed, and cells within these mesenchymal condensations either directly differentiate into osteoblasts and give origin to intramembranous bone, or differentiate into chondrocytes and form a cartilaginous anlage. The cartilaginous anlage or fetal growth plate is then replaced with bone. This process is also called endochondral bone development, and it is responsible for the generation of most of our skeleton. Here we discuss in detail the most common in vivo and in vitro techniques our laboratory is currently using for the analysis of the mouse fetal growth plate during development.
authors.affiliation: Department of Orthopaedic Surgery, Department of Medicine, Division of Endocrinology and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan.
authors.forename: Laura
authors.initials: L
authors.lastname: Mangiavini
authors.affiliation: Department of Orthopaedic Surgery, Department of Medicine, Division of Endocrinology and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan.|Inserm, UMRS 791-LIOAD, Centre for Osteoarticular and Dental Tissue Engineering, Group STEP 'Skeletal Tissue Engineering and Physiopathology', Nantes, France.|Faculty of Dental Surgery, LUNAM, Nantes University, Nantes, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Merceron
authors.affiliation: Department of Orthopaedic Surgery, Department of Medicine, Division of Endocrinology and Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan.
authors.forename: Ernestina
authors.initials: E
authors.lastname: Schipani
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D049109:Cell Proliferation; D015925:Cryopreservation; D003584:Cytological Techniques / Q000379:methods*; D004210:Dissection; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005333:Fetus; D005456:Fluorescent Dyes / Q000378:metabolism; D006132:Growth Plate / Q000166:cytology / Q000196:embryology / Q000254:growth & development*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D016612:Paraffin Embedding; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013194:Staining and Labeling; D016707:Tissue Fixation
chemical_list: D005456:Fluorescent Dyes; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger
keywords: cartilage; cell culture; endochondral bone development; mouse; staining