title: Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM.
abstract: Usually packed red blood cells (pRBCs) require specific conditions in storage procedures to ensure the maximum shelf life of up to 42 days in 2-6°C. However, molecular and biochemical consequences can affect the stored blood cells; these changes are collectively labeled as storage lesions. In this study, the effect of prolonged storage was assessed through investigating morphological changes and evaluating oxidative stress. Samples from leukodepleted pRBC in SAGM stored at 4°C for 42 days were withdrawn aseptically on day 0, day 14, day 28, and day 42. Morphological changes were observed using scanning electron microscopy and correlated with osmotic fragility and hematocrit. Oxidative injury was studied through assessing MDA level as a marker for lipid peroxidation. Osmotic fragility test showed that extended storage time caused increase in the osmotic fragility. The hematocrit increased by 6.6% from day 0 to day 42. The last 2 weeks show alteration in the morphology with the appearance of echinocytes and spherocytes. Storage lesions and morphological alterations appeared to affect RBCs during the storage period. Further studies should be performed to develop strategies that will aid in the improvement of stored pRBC quality and efficacy.
authors.affiliation: Health Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors.forename: Ibrahim
authors.identifier: 0000-0001-8702-8249
authors.initials: I
authors.lastname: Mustafa
authors.affiliation: Hematology Department, Hamad General Hospital, Hamad Medical Corporation, Doha, Qatar.
authors.forename: Asma
authors.identifier: 0000-0001-9966-3741
authors.initials: A
authors.lastname: Al Marwani
authors.affiliation: Health Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors.forename: Khuloud
authors.identifier: 0000-0002-0428-9994
authors.initials: K
authors.lastname: Mamdouh Nasr
authors.affiliation: Health Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors.forename: Noora
authors.identifier: 0000-0003-2485-5786
authors.initials: N
authors.lastname: Abdulla Kano
authors.affiliation: Health Sciences Department, College of Arts and Sciences, Qatar University, Doha, Qatar.
authors.forename: Tameem
authors.identifier: 0000-0001-9955-1088
authors.initials: T
authors.lastname: Hadwan
mesh_terms: D004912:Erythrocytes / Q000737:chemistry / Q000648:ultrastructure*; D006400:Hematocrit / Q000379:methods; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009996:Osmotic Fragility; D018384:Oxidative Stress; D013048:Specimen Handling