title: [Study on HPLC-DAD Fingerprint of Xiaoying Jiedu Decoction].
abstract: OBJECTIVE
  Based on backtracking method, identification and quality evaluation of Xiaoying Jiedu Decoction (XYJDD) by HPLC fingerprint analysis were carried out.
  
  
  METHODS
  HPLC-DAD fingerprint of XYJDD was conducted with Dikma Platisil ODS C18 column (250 mm x 4.6 mm, 5 µm) and gradient elution with the mobile phase consisting of acetonitrile-(0.1% phosphoric acid-0.1% triethylamine solution) at column temperature of 30 °C. On the basis of the established chromatographic pattern of XYJDD tracking backward to the corresponding crude herbal drugs in the formula and the attribution of most peaks in the XYJDD fingerprint can be figured out.
  
  
  RESULTS
  16 peaks of XYJDD HPLC fingerprint were assigned by parallel comparison with the fingerprint of the five corresponding crude drugs in the formula of Scutellariae Radix, Glycyrrhizae Radix et Rhizoma, Houttuyniae Herba, Aurantii Fructus, Arnebiae Radix. Four peaks can be identified with the chemical reference substances.
  
  
  CONCLUSIONS
  The entirety of XYJDD HPLC fingerprint enhances the specialty for control and assessment of the product quality, and the backtracking experimental method can be expected to be applied in chromatographic fingerprinting analysis of complex Chinese patent medicine.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cai
authors.forename: Yu
authors.initials: Y
authors.lastname: Lu
authors.forename: Rong-li
authors.initials: RL
authors.lastname: Qiu
authors.forename: Chong-fa
authors.initials: CF
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Gong
authors.forename: Yong-zhang
authors.initials: YZ
authors.lastname: Ji
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal