title: Genetic diversity of rhizobia nodulating native Vicia spp. in Sweden.
abstract: Despite the recognition that Rhizobium leguminosarum sv. viciae is the most common symbiont of Vicia species worldwide, there is no available information on rhizobia nodulating native Vicia species in Sweden. We have therefore studied the genetic diversity and phylogeny of root nodule bacteria isolated from V. cracca, V. hirsuta, V. sepium, V. tetrasperma and V. sylvatica growing in different locations in Sweden as well as an isolate each from V. cracca in Tromsø, Norway, and V. multicaulis in Siberia, Russia. Out of 25 isolates sampled from the six Vicia species in 12 different locations, there were 14 different genotypes based on the atpD, recA and nodA gene phylogenies. All isolates were classified into Rhizobium leguminosarum sv. viciae group based on the concatenated atpD and recA phylogeny and the nodA phylogeny.
authors.affiliation: Department of Agricultural Research for Northern Sweden, Swedish University of Agricultural Sciences (SLU), SE-90183 Umeå, Sweden. Electronic address: oseiampomah@yahoo.com.
authors.forename: Osei Yaw
authors.initials: OY
authors.lastname: Ampomah
authors.affiliation: Department of Agricultural Research for Northern Sweden, Swedish University of Agricultural Sciences (SLU), SE-90183 Umeå, Sweden.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Huss-Danell
mesh_terms: D000217:Acyltransferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D010802:Phylogeny; D011938:Rec A Recombinases / Q000235:genetics; D016961:Rhizobium leguminosarum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013548:Sweden; D014157:Transcription Factors / Q000235:genetics; D031306:Vicia / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014157:Transcription Factors; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales; D011938:Rec A Recombinases
keywords: Diversity; Nodulation; Phylogeny; Rhizobia; Vicia