title: Promoter regulatory domain identification of cassava starch synthase IIb gene in transgenic tobacco.
abstract: Soluble starch synthase is a key enzyme in the starch biosynthesis pathway, and its enzyme activity significantly influences starch components in cassava storage root. However, studies on the regulation mechanism of soluble starch synthase gene are rare. In this study, we cloned the 5' flanking sequence of the MeSSIIb gene and predicted the distribution of cis-elements. The region from -453 to -1 was considered the primary core promoter by the quantitative detection of GUS activity in transgenic tobacco plants containing 5' truncated promoters fused with the GUS gene. Analysis results clarified that the region from -531 to -454 significantly repressed promoter activity. The region from -453 to -388 was a repressive domain of ethylene, and some unknown drought responsive cis-elements were located in the region from -387 to -1. These findings will provide useful information on the functional assay and transcriptional regulation mechanisms of the MeSSIIb gene.
authors.affiliation: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, PR China; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, PR China. Electronic address: 1211374137@qq.com.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Guan
authors.affiliation: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, PR China; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, PR China. Electronic address: chenxin@itbb.org.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, PR China; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, PR China. Electronic address: 493971908@qq.com.
authors.forename: Hairong
authors.initials: H
authors.lastname: Xie
authors.affiliation: The Institute of Tropical Bioscience and Biotechnology (ITBB), Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, PR China; Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, PR China. Electronic address: wangwenquan@itbb.org.cn.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
mesh_terms: D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D002365:Manihot / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D013214:Starch Synthase / Q000096:biosynthesis / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics
chemical_list: D010940:Plant Proteins; D013214:Starch Synthase
keywords: Cis-element; GUS protein; Genetic transformation; Promoter; Soluble starch synthase