title: Physiological properties of Scomber japonicus meat hydrolysate prepared by subcritical water hydrolysis.
abstract: The health-beneficial biological activities, including antioxidant and tyrosinase inhibitory activities, of Scomber japonicus muscle protein hydrolysates prepared by subcritical water hydrolysis were investigated. After 5 min of subcritical hydrolysis at 140 degrees C, 59.76% of S. japonicus muscle protein was hydrolyzed, the highest degree of hydrolysis in all the groups were tested. According to the response surface methodology results, as the reaction temperature and reaction time became lower and shorter, the yield became higher. The highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity (90.63%) occurred in hydrolysates treated at 140 degrees C for 5 min, and the highest tyrosinase inhibitory activity (65.54%) was identified in hydrolysates treated at 200 degreesC for 15 min. Changes in the molecular weight distribution of S. japonicus muscle proteins after subcritical water hydrolysis were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Subcritical water hydrolysis is a suitable technique for obtaining S.japonicus muscle protein hydrolysates with useful biological activities, within a short time (5-15 min).
authors.forename: Jae-Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Hye Eun
authors.initials: HE
authors.lastname: Moon
authors.forename: Myong-Kyun
authors.initials: MK
authors.lastname: Roh
authors.forename: Yu-Mi
authors.initials: YM
authors.lastname: Ha
authors.forename: Bo-Bae
authors.initials: BB
authors.lastname: Lee
authors.forename: Kwang Keun
authors.initials: KK
authors.lastname: Cho
authors.forename: In Soon
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds; D005396:Fish Products / Q000032:analysis*; D005399:Fishes; D005511:Food Handling / Q000379:methods*; D016166:Free Radical Scavengers; D006868:Hydrolysis; D008461:Meat Products / Q000032:analysis*; D010851:Picrates; D014867:Water / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl