title: Transplantation of choroid plexus epithelial cells into contusion-injured spinal cord of rats.
abstract: The effect of the transplantation of choroid plexus epithelial cells (CPECs) on locomotor improvement and tissue repair including axonal extension in spinal cord lesions was examined in rats with spinal cord injury (SCI).
  
  
  
  CPECs were cultured from the choroid plexus of green fluorescent protein (GFP)-transgenic rats, and transplanted directly into the contusion-injured spinal cord lesions of rats of the same strain. Locomotor behaviors were evaluated based on BBB scores every week after transplantation until 4 weeks after transplantation. Histological and immunohistochemical examinations were performed at 2 days, and every week until 5 weeks after transplantation.
  
  
  
  Locomotor behaviors evaluated by the BBB score were significantly improved in cell-transplanted rats. Numerous axons grew, with occasional interactions with CPECs, through the astrocyte-devoid areas. These axons exhibited structural characteristics of peripheral nerves. GAP-43-positive axons were found at the border of the lesion 2 days after transplantation. Cavity formation was more reduced in cell-transplanted than control spinal cords. CPECs were found within the spinal cord lesion, and sometimes in association with astrocytes at the border of the lesion until 2 weeks after transplantation.
  
  
  
  The transplantation of CPECs enhanced locomotor improvement and tissue recovery, including axonal regeneration, in rats with SCI.
authors.affiliation: Institute of Regeneration and Rehabilitation, Aino University School of Health Science, Osaka, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kanekiyo
authors.affiliation: Institute of Regeneration and Rehabilitation, Aino University School of Health Science, Osaka, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Nakano
authors.affiliation: Department of Physical Therapy, Aino University School of Health Science, Osaka, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Noda
authors.affiliation: Department of Physical Therapy, Aino University School of Health Science, Osaka, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yamada
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tazuke Medical Research Institute, Kitano Hospital, Osaka, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tazuke Medical Research Institute, Kitano Hospital, Osaka, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ohta
authors.affiliation: Department of Orthopedic Surgery, Aino Hospital, Osaka, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yokota
authors.affiliation: Translational Research Informatics Center, Foundation for Biomedical Research and Innovation, Kobe, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Fukushima
authors.affiliation: Institute of Regeneration and Rehabilitation, Aino University School of Health Science, Osaka, Japan.
authors.forename: Chizuka
authors.initials: C
authors.lastname: Ide
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000473:pathology / Q000648:ultrastructure; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000166:cytology*; D003288:Contusions / Q000150:complications; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008124:Locomotion / Q000502:physiology; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000209:etiology / Q000473:pathology / Q000601:surgery*; D014162:Transfection
chemical_list: D019922:GAP-43 Protein; D005904:Glial Fibrillary Acidic Protein; D049452:Green Fluorescent Proteins
keywords: Spinal cord injury; axonal regeneration; choroid plexus epithelial cell; transplantation; trophic factor