title: [IFN-γ up-regulates PD-L1 expression in human placenta mesenchymal stem cells and enhances cell ability to induce the differentiation of IL-10+ T cells from cord blood- and peripheral blood-derived T cells].
abstract: OBJECTIVE
  To compare the differentiation, inducing effects of human placenta mesenchymal stem cells (hPMSCs) on IL-10(+) T cells derived from cord blood and peripheral blood, and investigate the effect of IFN-γ on the induction.
  
  
  METHODS
  The hPMSCs were isolated from human placenta and cultured. The expression of programmed death ligand 1 (PD-L1) in hPMSCs was detected by reverse transcriptase PCR and flow cytometry (FCM), respectively. Mononuclear cells were isolated from cord blood and peripheral blood of healthy donors by Ficoll density gradient centrifugation, and T cells were purified by sheep red blood cells. Then hPMSCs, pretreated with PD-L1 mAb or IFN-γ, were co-cultured with phytohaemagglutinin (PHA)-activated T cells. Percentages of CD4(+)IL-10(+) and CD8(+)IL-10(+) T cells in cord blood and peripheral blood T cells were analyzed by FCM.
  
  
  RESULTS
  hPMSCs could induce the differentiation of CD4(+)IL-10(+) and CD8(+)IL-10(+) T cells from cord blood or peripheral blood T cells, and the number of IL-10(+) T cells in the peripheral blood T cells was significantly higher than that in the cord blood T cells. Pretreatment with IFN-γ markedly enhanced the differentiation, inducing ability of hPMSCs. PD-L1 was highly expressed in hPMSCs, and the expression was also significantly promoted by IFN-γ. After the expression of PD-L1 was blocked in hPMSCs, the percentages of CD4(+)IL-10(+) and CD8(+)IL-10(+) T cells obviously decreased in cord blood and peripheral blood T cells.
  
  
  CONCLUSIONS
  The ability of hPMSCs to induce the differentiation of IL-10(+) T cells from peripheral blood T cells was apparently stronger than that in cord blood T cells. IFN-γ could up-regulate the number of IL-10(+)T cells differentiated from cord blood and peripheral blood T cells in the present of hPMSCs by enhancing the expression of PD-L1 in hPMSCs.
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Fenghuang
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Gynecology and Obstetrics, Laishan First People's Hospital, Yantai 264003, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: DU
authors.affiliation: Yantai Center Blood Station, Yantai 264003, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Junzhu
authors.initials: J
authors.lastname: Yi
authors.affiliation: Department of Laboratory Medicine, Yantai Hospital Affiliated to Binzhou Medical University, Yantai 264003, China.
authors.forename: Guoyan
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai 264003, China. *Corresponding author, E-mail: xyluan@ sohu.com.
authors.forename: Xiying
authors.initials: X
authors.lastname: Luan
mesh_terms: D000328:Adult; D000818:Animals; D060890:B7-H1 Antigen / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010920:Placenta / Q000166:cytology; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human; D016753:Interleukin-10; D007371:Interferon-gamma