title: Effect of 4-week feeding of deoxynivalenol- or T-2-toxin-contaminated diet on lipid peroxidation and glutathione redox system in the hepatopancreas of common carp (Cyprinus carpio L.).
abstract: The purpose of study was to investigate the effects of T-2 toxin (4.11 mg T-2 toxin and 0.45 mg HT-2 toxin kg(-1) feed) and deoxynivalenol (5.96 and 0.33 mg 15-acetyl deoxynivalenol (DON) kg(-1) feed) in 1-year-old common carp juveniles in a 4-week feeding trial. The exposure of mycotoxins resulted in increased mortality in both groups consuming mycotoxin-contaminated diet. Parameters of lipid peroxidation were not affected during the trial, and antioxidant defence also did not show response to oxidative stress; however, glutatione peroxidase activity slightly, but significantly, decreased in the T-2 toxin group. Glutathione S-transferase activity showed moderate decrease as effect of T-2 toxin, which suggests its effect on xenobiotic transformation. Reduced glutathione concentration showed moderate changes as effect of DON exposure, but T-2 toxin has no effect. Expression of phospholipid hydroperoxide glutathione peroxidase (GPx4) genes showed different response to mycotoxin exposure. T-2 toxin caused dual response in the expression of gpx4a (early and late downregulation and mid-term upregulation), but continuous upregulation was found as effect of deoxynivalenol. Expression of the other gene, gpx4b, was upregulated by both trichothecenes during the whole period. The results suggested that trichothecenes have some effect on free radical formation and antioxidant defence, but the changes depend on the duration of exposure and the dose applied, and in case of glutathione peroxidase, there was no correlation between expression of genes and enzyme activity.
authors.affiliation: Department of Nutrition, Szent István University, 2103, Gödöllő, Hungary.
authors.forename: Csilla
authors.initials: C
authors.lastname: Pelyhe
authors.affiliation: Department of Nutrition, Szent István University, 2103, Gödöllő, Hungary.
authors.forename: Benjámin
authors.initials: B
authors.lastname: Kövesi
authors.affiliation: Mycotoxins in the Food Chain Research Group, Hungarian Academy of Sciences-Kaposvár University, 7400, Kaposvár, Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Zándoki
authors.affiliation: Department of Aquaculture, Szent István University, 2103, Gödöllő, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kovács
authors.affiliation: Mycotoxins in the Food Chain Research Group, Hungarian Academy of Sciences-Kaposvár University, 7400, Kaposvár, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Szabó-Fodor
authors.affiliation: Department of Nutrition, Szent István University, 2103, Gödöllő, Hungary. Mezes.Miklos@mkk.szie.hu.
authors.forename: Miklós
authors.initials: M
authors.lastname: Mézes
authors.affiliation: Department of Nutrition, Szent István University, 2103, Gödöllő, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Balogh
mesh_terms: D000821:Animal Feed / Q000009:adverse effects / Q000032:analysis*; D000818:Animals; D002347:Carps / Q000378:metabolism*; D005506:Food Contamination / Q000032:analysis; D005978:Glutathione / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D009183:Mycotoxins / Q000378:metabolism* / Q000633:toxicity; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D013605:T-2 Toxin / Q000378:metabolism* / Q000633:toxicity; D014255:Trichothecenes / Q000378:metabolism* / Q000633:toxicity
chemical_list: D009183:Mycotoxins; D014255:Trichothecenes; D005978:Glutathione; D013605:T-2 Toxin; C007262:deoxynivalenol
keywords: Common carp; Deoxynivalenol; Glutathione; Lipid peroxidation; T-2 toxin