title: Equine herpesvirus type 1 (EHV1) induces alterations in the immunophenotypic profile of equine monocyte-derived dendritic cells.
abstract: Equine herpesvirus 1 (EHV1) is an α-herpesvirus that can infect a variety of different cells in vitro and in vivo, including dendritic cells (DC) which are essential in the immune response against EHV1. Infection of equine monocyte-derived DC (MDDC) with EHV1 induced down-regulation of major histocompatibility complex I (MHCI), CD83, CD86, CD206, CD29 and CD172a, but not of CD11a/CD18 and MHCII. This down-regulation was not mediated by the virion host-shutoff (VHS) protein or pUL49.5. Interestingly, down-regulation of CD83 and CD86 was in part mediated by pUL56. Taken together, these data indicate that EHV1 employs different and still unresolved mechanisms to induce down-regulation of several functionally important cell surface proteins on equine DC.
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Claessen
authors.affiliation: Department of Obstetrics, Reproduction and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
authors.forename: Valérie
authors.initials: V
authors.lastname: De Lange
authors.affiliation: Institut für Virologie, Freie Universität Berlin, 14163 Berlin, Germany.
authors.forename: Teng
authors.initials: T
authors.lastname: Huang
authors.affiliation: Institut für Virologie, Freie Universität Berlin, 14163 Berlin, Germany.
authors.forename: Guanggang
authors.initials: G
authors.lastname: Ma
authors.affiliation: Institut für Virologie, Freie Universität Berlin, 14163 Berlin, Germany.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Osterrieder
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
authors.forename: Herman
authors.initials: H
authors.lastname: Favoreel
authors.affiliation: Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium; Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA. Electronic address: grv23@cornell.edu.
authors.forename: Gerlinde R
authors.initials: GR
authors.lastname: Van de Walle
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D006736:Horses; D016130:Immunophenotyping; D008297:Male
chemical_list: D000943:Antigens, Differentiation
keywords: Down-regulation; Equine herpesvirus 1; Monocyte-derived dendritic cells; pUL56