title: Method Based on the β-Lactamase PenPC Fluorescent Labeled for β-Lactam Antibiotic Quantification in Human Plasma.
abstract: Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.
authors.affiliation: Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Max
authors.initials: M
authors.lastname: Andresen
authors.affiliation: State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Kwok-Yin
authors.initials: KY
authors.lastname: Wong
authors.affiliation: State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Yun-Chung
authors.initials: YC
authors.lastname: Leung
authors.affiliation: State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Wai-Ting
authors.initials: WT
authors.lastname: Wong
authors.affiliation: State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Pak-Ho
authors.initials: PH
authors.lastname: Chan
authors.affiliation: Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Max
authors.initials: M
authors.lastname: Andresen-Vasquez
authors.affiliation: Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Leyla
authors.initials: L
authors.lastname: Alegria
authors.affiliation: Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Camila
authors.initials: C
authors.lastname: Silva
authors.affiliation: Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Tapia
authors.affiliation: Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Patricio
authors.initials: P
authors.lastname: Downey
authors.affiliation: Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Dagoberto
authors.initials: D
authors.lastname: Soto
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D015374:Biosensing Techniques / Q000379:methods*; D016903:Drug Monitoring; D005453:Fluorescence; D064368:Healthy Volunteers; D006801:Humans; D000077731:Meropenem; D034421:Sus scrofa / Q000382:microbiology; D013845:Thienamycins / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D001618:beta-Lactamases / Q000097:blood; D047090:beta-Lactams / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D013845:Thienamycins; D047090:beta-Lactams; D001618:beta-Lactamases; D000077731:Meropenem