title: Quantification of permanent positively charged compounds in plasma using one-step dilution to reduce matrix effect in MS.
abstract: BACKGROUND
  Bioanalysis of conventional methods for compounds with permanent positive charge leads to peak tailing in separation and matrix effects in MS. This study describes a novel, rapid and sensitive method for quinolinium-containing compounds quantification. Results & methodology: A charged surface hybrid chromatography-tandem MS/MS using one-step protein precipitation dilution technique has been developed for determining analytes in plasma. We found symmetric peak and high recoveries for the analytes without matrix effect. All calibration curves had good linearity (r 0.991). The intra- and inter-assay precision was within 15% and the accuracy ranged from 88 to 103%. The method has been successfully applied to the PK study.
  
  
  CONCLUSIONS
  The proposed method was sensitive, reproducible and applicable to other permanent positively charged compounds.
authors.affiliation: Drug Development Core, Faculty of Health Sciences, University of Macau, Macau SAR, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Drug Development Core, Faculty of Health Sciences, University of Macau, Macau SAR, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.affiliation: Drug Development Core, Faculty of Health Sciences, University of Macau, Macau SAR, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Drug Development Core, Faculty of Health Sciences, University of Macau, Macau SAR, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Hu
authors.affiliation: Drug Development Core, Faculty of Health Sciences, University of Macau, Macau SAR, PR China.
authors.forename: Kin Yip
authors.initials: KY
authors.lastname: Tam
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D051379:Mice; D011806:Quinolinium Compounds / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry
chemical_list: D014150:Antipsychotic Agents; D011806:Quinolinium Compounds
keywords: PK; charged surface hybrid chromatography; matrix effect; one-step protein precipitation dilution technique; permanent positively charged compounds; tail vessel microsampling