title: In Vivo Models to Study Chemokine Biology.
abstract: Chemokines are essential mediators of leukocyte movement in vivo. In vitro assays of leukocyte migration cannot mimic the complex interactions with other cell types and matrix needed for cells to extravasate and migrate into tissues. Therefore, in vivo strategies to study the effects and potential relevance of chemokines for the migration of particular leukocyte subsets are necessary. Here, we describe methods to study the effects and endogenous role of chemokine in mice. Advantages and pitfalls of particular models are discussed and we focus on description in model's joint and pleural cavity inflammation and the effects and relevance of CXCR2 and CCR2 ligands on cell migration.
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: F A
authors.initials: FA
authors.lastname: Amaral
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Boff
authors.affiliation: Immunopharmacology, Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: mmtex@icb.ufmg.br.
authors.forename: M M
authors.initials: MM
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism*; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000378:metabolism; D054360:Chemokine CXCL1 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D007596:Joints / Q000473:pathology; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D035422:Pleural Cavity / Q000378:metabolism / Q000473:pathology; D054390:Receptors, CCR2 / Q000378:metabolism; D023063:Receptors, Interleukin-8B / Q000008:administration & dosage / Q000378:metabolism
chemical_list: C515335:Ccr2 protein, mouse; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D018925:Chemokines; D054390:Receptors, CCR2; D023063:Receptors, Interleukin-8B
keywords: Arthritis; CCR2; CXCR2; Cell recruitment; Chemokine; In vivo models; Pleural cavity