title: Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing.
abstract: BACKGROUND
  Suaeda glauca, a succulent halophyte of the Chenopodiaceae family, is widely distributed in coastal areas of China. Suaeda glauca is highly resistant to salt and alkali stresses. In the present study, the salt-responsive transcriptome of Suaeda glauca was analyzed to identify genes involved in salt tolerance and study halophilic mechanisms in this halophyte.
  
  
  RESULTS
  Illumina HiSeq 2500 was used to sequence cDNA libraries from salt-treated and control samples with three replicates each treatment. De novo assembly of the six transcriptomes identified 75,445 unigenes. A total of 23,901 (31.68%) unigenes were annotated. Compared with transcriptomes from the three salt-treated and three salt-free samples, 231 differentially expressed genes (DEGs) were detected (including 130 up-regulated genes and 101 down-regulated genes), and 195 unigenes were functionally annotated. Based on the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) classifications of the DEGs, more attention should be paid to transcripts associated with signal transduction, transporters, the cell wall and growth, defense metabolism and transcription factors involved in salt tolerance.
  
  
  CONCLUSIONS
  This report provides a genome-wide transcriptional analysis of a halophyte, Suaeda glauca, under salt stress. Further studies of the genetic basis of salt tolerance in halophytes are warranted.
authors.affiliation: Zhejiang Academy of Agricultural Science, Institute of Crops and Nuclear Technology Utilization, Hangzhou Zhejiang 310021, People's Republic of China.
authors.forename: Hangxia
authors.initials: H
authors.lastname: Jin
authors.affiliation: Zhejiang Academy of Agricultural Science, Institute of Crops and Nuclear Technology Utilization, Hangzhou Zhejiang 310021, People's Republic of China.
authors.forename: Dekun
authors.initials: D
authors.lastname: Dong
authors.affiliation: Zhejiang Academy of Agricultural Science, Institute of Crops and Nuclear Technology Utilization, Hangzhou Zhejiang 310021, People's Republic of China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Zhejiang Academy of Agricultural Science, Institute of Crops and Nuclear Technology Utilization, Hangzhou Zhejiang 310021, People's Republic of China.
authors.forename: Danhua
authors.initials: D
authors.lastname: Zhu
mesh_terms: D019663:Chenopodiaceae / Q000235:genetics* / Q000502:physiology; D018744:DNA, Plant / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D015723:Gene Library; D060888:Real-Time Polymerase Chain Reaction; D055051:Salt-Tolerant Plants / Q000235:genetics* / Q000502:physiology; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D018744:DNA, Plant