title: Identification and molecular characterization of peroxiredoxin 6 from Japanese eel (Anguilla japonica) revealing its potent antioxidant properties and putative immune relevancy.
abstract: Peroxiredoxins (Prdx) are thiol specific antioxidant enzymes that play a pivotal role in cellular oxidative stress by reducing toxic peroxide compounds into nontoxic products. In this study, we identified and characterized a peroxiredoxin 6 counterpart from Japanese eel (Anguilla japonica) (AjPrdx6) at molecular, transcriptional and protein level. The identified full-length coding sequence of AjPrdx6 (669 bp) coded for a polypeptide of 223 aa residues (24.9 kDa). Deduced protein of AjPrdx6 showed analogy to characteristic structural features of 1-cysteine peroxiredoxin sub-family. According to the topology of the generated phylogenetic reconstruction AjPrdx6 showed closest evolutionary relationship with Salmo salar. As detected by Quantitative real time PCR (qPCR), AjPrdx6 mRNA was constitutively expressed in all the tissues examined. Upon the immune challenges with Edwardsiella tarda, lipopolysaccharides and polyinosinic:polycytidylic acid, expression of AjPrdx6 mRNA transcripts were significantly induced. The general functional properties of Prdx6 were confirmed using purified recombinant AjPrdx6 protein by deciphering its potent protective effects on cultured vero cells (kidney epithelial cell from an African green monkey) against H2O2-induced oxidative stress and protection against oxidative DNA damage elicited by mixed function oxidative (MFO) system. Altogether, our findings suggest that AjPrdx6 is a potent antioxidant protein in Japanese eels and its putative immune relevancy in pathogen stress mounted by live-bacteria or pathogen associated molecular patterns (PAMPs).
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: Thanthrige Thiunuwan
authors.initials: TT
authors.lastname: Priyathilaka
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: Yucheol
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: H M V
authors.initials: HMV
authors.lastname: Udayantha
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: Seongdo
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: H M L P B
authors.initials: HMLPB
authors.lastname: Herath
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea.
authors.forename: H H Chaminda
authors.initials: HHC
authors.lastname: Lakmal
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: Don Anushka Sandaruwan
authors.initials: DAS
authors.lastname: Elvitigala
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: Navaneethaiyer
authors.initials: N
authors.lastname: Umasuthan
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: G I
authors.initials: GI
authors.lastname: Godahewa
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Self-Governing Province 690-756, Republic of Korea; Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: Seong Il
authors.initials: SI
authors.lastname: Kang
authors.affiliation: Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea.
authors.forename: Hyung Bok
authors.initials: HB
authors.lastname: Jeong
authors.affiliation: New Strategy Research Center, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea.
authors.forename: Shin Kwon
authors.initials: SK
authors.lastname: Kim
authors.affiliation: New Strategy Research Center, National Fisheries Research and Development Institute, Busan 619-705, Republic of Korea. Electronic address: djkim4128@korea.kr.
authors.forename: Dae Jung
authors.initials: DJ
authors.lastname: Kim
authors.affiliation: Fish Vaccine Research Center, Jeju National University, Jeju Special Self-Governing Province 695-965, Republic of Korea. Electronic address: bslim@jejunu.ac.kr.
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Lim
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001483:Base Sequence; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics; D020609:Edwardsiella tarda; D004756:Enterobacteriaceae Infections / Q000276:immunology* / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects; D054465:Peroxiredoxin VI / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D011070:Poly I-C / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D016415:Sequence Alignment; D014709:Vero Cells
chemical_list: D000975:Antioxidants; D018076:DNA, Complementary; D029941:Fish Proteins; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide; D054465:Peroxiredoxin VI; D011070:Poly I-C
keywords: Anguilla japonica; Antioxidant; Oxidative stress; Peroxiredoxin 6