title: Enhanced anti-tuberculosis immunity by a TAT-Ag85B protein vaccine in a murine tuberculosis model.
abstract: BACKGROUND
  The development of more effective anti-tuberculosis vaccines would contribute to the control of the global problem of infection with Mycobacterium tuberculosis (MTB). Recently, increasing evidences showed that HIV-Tat protein transduction domain is implicated in promotion of vaccines by inducing cellular immuno-response. However, it is rare known about the role of TAT in vaccines against MTB.
  
  
  METHODS
  In this study, we expressed recombinant protein-fused Ag85B with TAT (TAT-Ag85B) which was used as a vaccine to inoculate mice infected with MTB.
  
  
  RESULTS
  As s result, both IgG2a in serum and IFN-γ or TNFα produced by spleen cells were all increased significantly in the mice inoculated by TAT-Ag85B. Furthermore, consistently, TAT-Ag85B inoculation significantly reduced MTB loads both in lung and spleen.
  
  
  CONCLUSIONS
  These findings demonstrate that a novel protein vaccine of TAT-Ag85B enhances immune response both in humoral and cellular immunity, and contributes to protective efficacy against MTB.
authors.affiliation: 1 Department of Medical Immunology, Medical School, Anhui University of Science and Technology , Huainan, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Dong
authors.forename: Wu
authors.initials: W
authors.lastname: Jing
authors.forename: Xing
authors.initials: X
authors.lastname: Yingru
authors.forename: Wang
authors.initials: W
authors.lastname: Wenyang
authors.forename: Cai
authors.initials: C
authors.lastname: Ru
authors.forename: Ni
authors.initials: N
authors.lastname: Shengfa
authors.forename: Xu
authors.initials: X
authors.lastname: Congjing
authors.forename: Dai
authors.initials: D
authors.lastname: Jingjing
authors.forename: Wang
authors.initials: W
authors.lastname: Wan
authors.forename: He
authors.initials: H
authors.lastname: Jiang
authors.forename: Zhang
authors.initials: Z
authors.lastname: Rongbo
mesh_terms: D000217:Acyltransferases / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology; D032581:Tuberculosis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D032581:Tuberculosis Vaccines; D054322:tat Gene Products, Human Immunodeficiency Virus; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis
keywords: Ag85B; Mycobacterium tuberculosis; Protective immunity; Protein transduction domain; TAT