title: [Expression of 17 beta-hydroxysteroid dehydrogenase type 1 in the kidney of rats: the capacity of the kidney for synthesizing sex hormones].
abstract: OBJECTIVE
  To investigate the expression of 17 beta-hydroxysteroid dehydrogenase type 1 (17β-HSD1) in the kidney of rats and explore the capacity of the kidney for synthesizing sex hormones.
  
  
  METHODS
  The expressions of 17-HSD1 and sex hormones were detected by Western blotting and radioimmunoassay in rat renal cells in primary cultured for 24 and 48 h in the presence or absence of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).
  
  
  RESULTS
  After cell culture for 24 h, the primary rat renal cells expressed a low level of 17β-HSD1 (0.1843±0.076), which increased to 1.6651±0.044 (P<0.01) in response to co-stimulation by FSH and LH. Low levels of estradiol, progesterone and testosterone were also detected in rat renal cells (3.30±3.78, 62.60±12.33, and 22.12±3.36, respectively), and co-stimulation of FSH and LH significantly increased their levels to 8.50±2.64, 117.80±9.79, and 45.04±4.39, respectively (P<0.05). The levels of these hormones showed no significant differences between cells cultured for 24 h and 48 h (P>0.05).
  
  
  CONCLUSIONS
  The rat renal cells express 17β-HSD1 and are capable of stably secreting sex hormones in response to co-stimulation with FSH and LH, suggesting the capacity of the rat kidneys for synthesizing sex hormones. These findings enrich the understanding of the endocrine function of the kidney.
authors.affiliation: Department of Traditional Chinese Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. E-mail: 531043394@qq.com.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hong-Zhu
authors.initials: HZ
authors.lastname: Wang
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Peng
authors.forename: Qing-Lian
authors.initials: QL
authors.lastname: Zheng
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D013739:Testosterone / Q000096:biosynthesis*
chemical_list: D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase