title: Increased Apoptosis of Inflamed Odontoblasts Is Associated with CD47 Loss.
abstract: Survival of odontoblasts during infection and inflammation determines prognosis of the dental pulp. CD47 is a "self"-label surface marker on viable cells. The aim of the present study is to investigate the interaction between CD47, autophagy, and apoptosis in inflamed human dental pulp and lipopolysaccharide (LPS)-treated mDPC6T cells. We identified activation of autophagy and apoptosis in the odontoblasts due to inflammation of the human dental pulp. Furthermore, downregulation of CD47 correlated with increased autophagy and apoptosis in dental pulp of the patients afflicted with either caries and/or pulpitis. We also detected colocalization of CD47 and LC3 in the inflamed odontoblasts. In addition, functional study indicated that loss of CD47 activates autophagy and increases apoptosis, indicating that CD47 plays a key role in the LPS-induced autophagy and apoptosis of odontoblasts.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOS and Key Laboratory for Oral Biomedicine of Ministry of Education [KLOBM]), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: H S
authors.initials: HS
authors.lastname: Wang
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOS and Key Laboratory for Oral Biomedicine of Ministry of Education [KLOBM]), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: F
authors.initials: F
authors.lastname: Pei
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOS and Key Laboratory for Oral Biomedicine of Ministry of Education [KLOBM]), School and Hospital of Stomatology, Wuhan University, Wuhan, China Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOS and Key Laboratory for Oral Biomedicine of Ministry of Education [KLOBM]), School and Hospital of Stomatology, Wuhan University, Wuhan, China Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, China zhichen@whu.edu.cn luzhang2012@whu.edu.cn.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D001343:Autophagy / Q000502:physiology; D015153:Blotting, Western; D051928:CD47 Antigen / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D003731:Dental Caries / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology; D015536:Down-Regulation; D006801:Humans; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D009804:Odontoblasts / Q000378:metabolism*; D011671:Pulpitis / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D051928:CD47 Antigen; C497894:CD47 protein, human; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D053148:Caspase 3
keywords: autophagy; caries; inflammation; light chain 3; lipopolysaccharide; preodontoblast