title: Methylation profiling by bisulfite sequencing analysis of the mtDNA Non-Coding Region in replicative and senescent Endothelial Cells.
abstract: The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5 mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5 mC clusters and a biased distribution of 5 mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5 mC in D-loop formation, rather than in mitochondrial gene expression.
authors.affiliation: Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino (CCM), IRCCS, Milano, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Bianchessi
authors.affiliation: Centro Cardiologico Monzino (CCM), IRCCS, Milano, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Vinci
authors.affiliation: Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino (CCM), IRCCS, Milano, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Nigro
authors.affiliation: Genomics Core, Parco Tecnologico Padano, Lodi, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Rizzi
authors.affiliation: Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino (CCM), IRCCS, Milano, Italy.
authors.forename: Floriana
authors.initials: F
authors.lastname: Farina
authors.affiliation: Laboratory of Vascular Pathology, Istituto Dermopatico dell'Immacolata (IDI), IRCCS, Roma, Italy.
authors.forename: Maurizio C
authors.initials: MC
authors.lastname: Capogrossi
authors.affiliation: Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino (CCM), IRCCS, Milano, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Pompilio
authors.affiliation: Genomics Core, Parco Tecnologico Padano, Lodi, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Gualdi
authors.affiliation: Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino (CCM), IRCCS, Milano, Italy. Electronic address: andlauri@libero.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lauri
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D021901:DNA, Intergenic / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000737:chemistry*; D042783:Endothelial Cells / Q000737:chemistry*; D006801:Humans; D008928:Mitochondria / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods; D013447:Sulfites / Q000378:metabolism
chemical_list: D021901:DNA, Intergenic; D004272:DNA, Mitochondrial; D013447:Sulfites; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; C042345:hydrogen sulfite
keywords: Bisulfite sequencing; D-loop; Endothelial Cell; Methylation; Senescence; mtDNA