title: Study of optimization of wastewater contaminant removal along with extracellular polymeric substances (EPS) production by a thermotolerant Bacillus sp. ISTVK1 isolated from heat shocked sewage sludge.
abstract: The present work involved study of wastewater contaminant removal along with EPS production by a thermotolerant bacterium Bacillus sp. ISTVK1, isolated from heat shocked sewage sludge. EPS production in basal and mineral medium containing 50% filter sterilized wastewater and 0.5% sucrose was found to be 0.83±0.12gL(-1) and 0.31±0.10gL(-1) culture, respectively. GC-MS analysis of EPS revealed the presence of β-d-glucose, α-d-galactose and β-d-arabinose. FT-IR spectrum confirmed the presence carbohydrates. Box-Behnken design was used to optimize process parameters for enhanced EPS production along with % COD reduction of wastewater. The optimised conditions when used in a 1.5L bioreactor showed EPS production of 1.67±0.06gL(-1) culture and 93.0±0.21 % COD removal.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Asmita
authors.initials: A
authors.lastname: Gupta
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: isthakur@hotmail.com.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
mesh_terms: D001407:Bacillus / Q000302:isolation & purification* / Q000378:metabolism*; D019149:Bioreactors; D002241:Carbohydrates; D005690:Galactose; D005947:Glucose; D006358:Hot Temperature; D011108:Polymers; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D012722:Sewage / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D002241:Carbohydrates; D005690:Galactose; D005947:Glucose; D011108:Polymers; D011135:Polysaccharides, Bacterial; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical; C085953:exopolysaccharide, Bacillus
keywords: Bacillus sp.; Bioreactor; Extracellular polymeric substances; Response surface methodology; Wastewater treatment