title: A Novel Conductometric Urea Biosensor with Improved Analytical Characteristic Based on Recombinant Urease Adsorbed on Nanoparticle of Silicalite.
abstract: Development of a conductometric biosensor for the urea detection has been reported. It was created using a non-typical method of the recombinant urease immobilization via adsorption on nanoporous particles of silicalite. It should be noted that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, and high reproducibility (RSD = 5.1 %). The linear range of urea determination by using the biosensor was 0.05-15 mM, and a lower limit of urea detection was 20 μM. The bioselective element was found to be stable for 19 days. The characteristics of recombinant urease-based biomembranes, such as dependence of responses on the protein and ion concentrations, were investigated. It is shown that the developed biosensor can be successfully used for the urea analysis during renal dialysis.
authors.affiliation: Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. taras.velychko@gmail.com.|Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003, Kyiv, Ukraine. taras.velychko@gmail.com.
authors.forename: T P
authors.initials: TP
authors.lastname: Velychko
authors.affiliation: Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. alex_sold@yahoo.com.
authors.forename: О О
authors.initials: ОО
authors.lastname: Soldatkin
authors.affiliation: Department of Electrical and Magnetic Measurements, Institute of Electrodynamics of National Academy of Sciences of Ukraine, 56, Peremohy Ave., Kyiv-57, 03680, Ukraine. melnik@gmail.com.
authors.forename: V G
authors.initials: VG
authors.lastname: Melnyk
authors.affiliation: Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. svmarchenkosv@ukr.net.
authors.forename: S V
authors.initials: SV
authors.lastname: Marchenko
authors.affiliation: Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531, Turkey. kaankirde@gmail.com.|Central Laboratory, Middle East Technical University, Ankara, 06531, Turkey. kaankirde@gmail.com.
authors.forename: S K
authors.initials: SK
authors.lastname: Kirdeciler
authors.affiliation: Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531, Turkey. akata@metu.edu.tr.|Central Laboratory, Middle East Technical University, Ankara, 06531, Turkey. akata@metu.edu.tr.
authors.forename: B
authors.initials: B
authors.lastname: Akata
authors.affiliation: Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. a_soldatkin@yahoo.com.|Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003, Kyiv, Ukraine. a_soldatkin@yahoo.com.
authors.forename: A P
authors.initials: AP
authors.lastname: Soldatkin
authors.affiliation: Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. elskaya@imbg.org.ua.
authors.forename: A V
authors.initials: AV
authors.lastname: El'skaya
authors.affiliation: Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. dzyad@yahoo.com.|Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003, Kyiv, Ukraine. dzyad@yahoo.com.
authors.forename: S V
authors.initials: SV
authors.lastname: Dzyadevych
keywords: Biosensor; Conductometry; Enzyme; Recombinant urease; Silicalite