title: Comparative proteomics analysis of apoptotic Spodoptera frugiperda cells during p35 knockout Autographa californica multiple nucleopolyhedrovirus infection.
abstract: Infection with Autographa californica multiple nucleopolyhedrovirus (AcMNPV) mutants lacking a functional p35 gene can induce host cell apoptosis, which provides the possibility to use the potential of these viruses in the biological control of pest insects. Nonetheless, the proteomics or the protein changes of Spodoptera frugiperda (Sf9) cells infected with p35 knockout AcMNPV have not yet been studied. To further improve the use of AcMNPV, we set out to analyze the protein composition and protein changes of Sf9 cells of different infection stages by isobaric tag for relative and absolute quantification (iTRAQ) techniques. A total of 4004 sf9 proteins were identified by iTRAQ. After comparation of the significantly expressed 483 proteins from p35koAcMNPV-infected Sf9 cells and the significantly expressed 413 proteins from wtAcMNPV-infected Sf9 cells, we found that 226 proteins were specific to p35koAcMNPV-infected Sf9 cells. The 226 proteins were categorized according to GO classification for insects and were categorized into: biological processes, molecular functions and cellular components. Of interest, the most up-regulated proteins related to Epstein-Barr virus infection, RNA transport, Calcium signaling pathway, cGMP-PKG signaling pathway, oxidative phosphorylation and N-Glycan biosynthesis. Determination of the protein changes in p35 knockout AcMNPV-infected Sf9 cells would facilitate the better use of this virus-host cell interaction in pest insect control and other related fields.
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China. Electronic address: qianyucn@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China.
authors.forename: Jianliang
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China.
authors.forename: Yuanxin
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: College of Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangdong, 510225, PR China.
authors.forename: Dongling
authors.initials: D
authors.lastname: Wen
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D019295:Computational Biology; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D061987:Sf9 Cells; D015398:Signal Transduction; D018411:Spodoptera / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics*
chemical_list: D020543:Proteome; D014764:Viral Proteins; C081618:p35 protein, Nucleopolyhedrovirus
keywords: AcMNPV; Apoptosis; Sf9 cell; iTRAQ; p35 knockout