title: [Clinical Significance of Monitoring T Lymphocyte Subsets after Allogeneic Hematopoietic Stem Cell Transplantation].
abstract: OBJECTIVE
  To evaluate the relationship between T lymphocyte subsets and the incidence of graft-versus-host disease (GVHD) and its clinical significance of monitoring the changes of T lymphocyte subsets dynamicly on 1, 3, 6, 12 month after allogeneic hematopoietic stem cell transplantation (allo-HSCT).
  
  
  METHODS
  Twenty cases received allo-HSCT in Department of Hematology of General Hospital of Beijing Military Command from January 2013 to January 2014, including 10 males and 10 females with average age of 20.3 years (3-46 years old), among them 4 cases rectived HLA matched transplantation and 16 cases rectived HLA mismatched transplantation. The levels of T lymphocyte subsets including CD3(+), CD4(+), CD8(+), CD4(+)/CD8(+), CD4(+)CD25(high) FOXP3(+) in the peripheral blood were manitored with flow cytometry (FCM) on +1, +3, +6, +12 month after transplantation dynamicly.
  
  
  RESULTS
  (1) Follow up to March 2015, the levers of CD3(+), CD4(+), CD8(+), CD4(+)/CD8(+), CD4(+) CD25(high) FOXP3(+) showed a different degree of recovery after transplantation for all cases and returned to the lever of pre-transplantation on 12 month basically, and CD8(+) T cells recovered earlier than CD4(+) T cells, while the decrease of CD4(+) T cells lasted more than 1 year; The proportion inversion of CD4(+)/CD8(+) also lasted for more than 1 year;(2) The level of CD4(+) CD25(high) FOXP3(+) in patients with acute GVHD was lower than that in patients without acute GVHD.
  
  
  CONCLUSIONS
  The dynamic monitoring of the T lymphocyte subsets, especially CD4(+) CD25(high) FOXP3(+) after transplantation has importent clinical significance, it can forecast the incidence of acute GVHD before symptoms appeared; the dynamic monitoring of the T-lymphocyte subsets also can be used as reference indicator for prediction of GVHD, theraby it can reduce mortality of patients after transplantation.
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.|Clinical Medical College of Anhui Medical University, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Tong
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Jing-Xing
authors.initials: JX
authors.lastname: Lou
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Liu
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Xue-Peng
authors.initials: XP
authors.lastname: He
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Hematology, General Hospital of Beijing Military Command, Beijing 100700, China. E-mail: chenhui-ren@ medmail.com.cn.
authors.forename: Hui-Rren
authors.initials: HR
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006086:Graft vs Host Disease; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000166:cytology*; D055815:Young Adult