title: Cellulosimicrobium marinum sp. nov., an actinobacterium isolated from sea sediment.
abstract: A novel Gram stain positive actinobacterium, designated RS-7-4(T), was isolated from a sea sediment sample collected in Indonesia, and its taxonomic position was investigated using a polyphasic approach. Strain RS-7-4(T) was observed to form vegetative hyphae in the early phase of growth, but the hyphae eventually fragmented into short rods to coccoid cells. Growth occurred at 15-37 °C, pH 6.0-11.0 and in the presence of 0-7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain RS-7-4(T) was closely related to the members of the genus Cellulosimicrobium, with a similarity range of 98.08-99.10 %. The peptidoglycan type of strain RS-7-4(T) was found to be A4α L-Lys-L-Thr-D-Asp. The predominant menaquinone was MK-9(H4), and the major fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0. The DNA G+C content was 75.6 mol%. These chemotaxonomic features corresponded to those of the genus Cellulosimicrobium. Meanwhile, the results of DNA-DNA hybridization, and physiological and biochemical tests revealed that strain RS-7-4(T) was different from the recognized species of the genus Cellulosimicrobium. Therefore, strain RS-7-4(T) represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium marinum sp. nov. is proposed. The type strain is RS-7-4(T) (=NBRC 110994(T) =InaCC A726(T)).
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan. hamada-moriyuki@nite.go.jp.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shibata
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Indonesian Institute of Sciences (LIPI), Jl, Raya Jakarta-Bogor Km. 46, Cibinong, 16911, Indonesia.
authors.forename: Arif
authors.initials: A
authors.lastname: Nurkanto
authors.affiliation: Indonesian Institute of Sciences (LIPI), Jl, Raya Jakarta-Bogor Km. 46, Cibinong, 16911, Indonesia.
authors.forename: Shanti
authors.initials: S
authors.lastname: Ratnakomala
authors.affiliation: Indonesian Institute of Sciences (LIPI), Jl, Raya Jakarta-Bogor Km. 46, Cibinong, 16911, Indonesia.
authors.forename: Puspita
authors.initials: P
authors.lastname: Lisdiyanti
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan.
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S
keywords: Actinobacteria; Cellulosimicrobium marinum; Micrococcineae; Polyphasic taxonomy; Sea sediment