title: Developing a biohybrid lung - sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes.
abstract: Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
authors.affiliation: Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany; Member of the German Centre for Lung Research (DZL), Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, Hannover, Germany. Electronic address: wiegmann.bettina@mh-hannover.de.
authors.forename: Bettina
authors.initials: B
authors.lastname: Wiegmann
authors.affiliation: Institute of Turbomachinery and Fluid Dynamics, Department of Mechanical Engineering, Leibniz University Hannover, Germany.
authors.forename: Heide
authors.initials: H
authors.lastname: von Seggern
authors.affiliation: Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Höffler
authors.affiliation: Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany.
authors.forename: Sotirios
authors.initials: S
authors.lastname: Korossis
authors.affiliation: Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany.
authors.forename: Daniele
authors.initials: D
authors.lastname: Dipresa
authors.affiliation: Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Pflaum
authors.affiliation: Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Schmeckebier
authors.affiliation: Institute of Turbomachinery and Fluid Dynamics, Department of Mechanical Engineering, Leibniz University Hannover, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Seume
authors.affiliation: Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany; Member of the German Centre for Lung Research (DZL), Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, Hannover, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Haverich
mesh_terms: D000475:Alkenes; D001187:Artificial Organs; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D008168:Lung
chemical_list: D000475:Alkenes; C410454:1-pentene
keywords: Bioartificial Lung; Biohybrid lung; Endothelialization; Gas exchange membranes