title: A highly sensitive and selective impedimetric aptasensor for interleukin-17 receptor A.
abstract: Interleukin-17 receptor A (IL-17RA) has been recognized as a valuable biomarker for diverse diseases, including autoimmune diseases. In this work, an electrochemical biosensor with great sensitivity and selectivity toward IL-17RA was fabricated using an IL-17RA aptamer (Kd=14.00nM) for the first time. The aptasensor was manufactured using electrodeposition of gold nanoparticles, and then quantitative detection of IL-17RA was performed based on impedimetry. The developed sensor exhibited a superior analytical performance for IL-17RA with a wide dynamic range of 10-10,000pg/mL in buffer and a detection limit of 2.13pg/mL, which is lower than that of commercially available ELISA kits. In addition, we validated the high specificity of the designed aptasensor to only IL-17RA, which showed good sensitivity even in human serum solution. Furthermore, the detection of the differentiated HL-60 cells expressing IL-17RA was successfully performed. Clinical applicability of the sensor was also demonstrated utilizing neutrophils separated from asthma patients. It is expected that the fabricated aptasensor will become an excellent diagnostic platform for IL-17RA-mediated diseases.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: jhhsst@postech.ac.kr.
authors.forename: Hunho
authors.initials: H
authors.lastname: Jo
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: ksk9089@postech.ac.kr.
authors.forename: Seong-Kyeong
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: yhj1005@postech.ac.kr.
authors.forename: Hyungjun
authors.initials: H
authors.lastname: Youn
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: delighthyun@postech.ac.kr.
authors.forename: Heehyun
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: kwanghyunlee@postech.ac.kr.
authors.forename: Kwanghyun
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: jian1031@postech.ac.kr.
authors.forename: Jian
authors.initials: J
authors.lastname: Jeong
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: mokjihyun@postech.ac.kr.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Mok
authors.affiliation: Department of Allergy and Clinical Immunology, Ajou University School of Medicine, San-5, Woncheon-dong, Yeongtong-gu, Suwon 442-749, South Korea. Electronic address: kimsh@ajou.ac.kr.
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Allergy and Clinical Immunology, Ajou University School of Medicine, San-5, Woncheon-dong, Yeongtong-gu, Suwon 442-749, South Korea. Electronic address: hspark@ajou.ac.kr.
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Park
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology, 77, Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea. Electronic address: ciban@postech.ac.kr.
authors.forename: Changill
authors.initials: C
authors.lastname: Ban
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004595:Electroplating; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D053722:Receptors, Interleukin-17 / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D053722:Receptors, Interleukin-17; D006046:Gold
keywords: Aptamer; Autoimmune disease; Biosensor; IL-17RA; Impedance spectroscopy