title: Design, fabrication and characterization of oxidized alginate-gelatin hydrogels for muscle tissue engineering applications.
abstract: In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells cultured on hydrogels with OD of 30% showed that the highest rate of cell proliferation belong to hydrogel with OA/GEL weight ratio of 30/70. Overall, it can be concluded from all obtained results that the prepared hydrogel with OA/GEL weight ratio and OD of 30/70 and 30%, respectively, could be proper candidate for use in muscle tissue engineering.
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Baniasadi
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran mashayekhan@sharif.edu.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Mashayekhan
authors.affiliation: Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
authors.forename: Samira
authors.initials: S
authors.lastname: Fadaoddini
authors.affiliation: Translational Medicine Group, Mede Bioeconomy Company, Tehran, Iran.
authors.forename: Yasamin
authors.initials: Y
authors.lastname: Haghirsharifzamini
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D019245:Compressive Strength; D003432:Cross-Linking Reagents / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development*; D010084:Oxidation-Reduction; D013314:Stress, Mechanical; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D005780:Gelatin
keywords: Gelatin; Hydrogel; Muscle tissue engineering; Oxidized alginate; Wharton's jelly-derived mesenchymal stem cells