title: Proteomic identification of fat-browning markers in cultured white adipocytes treated with curcumin.
abstract: We previously reported that curcumin induces browning of primary white adipocytes via enhanced expression of brown adipocyte-specific genes. In this study, we attempted to identify target proteins responsible for this fat-browning effect by analyzing proteomic changes in cultured white adipocytes in response to curcumin treatment. To elucidate the role of curcumin in fat-browning, we conducted comparative proteomic analysis of primary adipocytes between control and curcumin-treated cells using two-dimensional electrophoresis combined with MALDI-TOF-MS. We also investigated fatty acid metabolic targets, mitochondrial biogenesis, and fat-browning-associated proteins using combined proteomic and network analyses. Proteomic analysis revealed that 58 protein spots from a total of 325 matched spots showed differential expression between control and curcumin-treated adipocytes. Using network analysis, most of the identified proteins were proven to be involved in various metabolic and cellular processes based on the PANTHER classification system. One of the most striking findings is that hormone-sensitive lipase (HSL) was highly correlated with main browning markers based on the STRING database. HSL and two browning markers (UCP1, PGC-1α) were co-immunoprecipitated with these markers, suggesting that HSL possibly plays a role in fat-browning of white adipocytes. Our results suggest that curcumin increased HSL levels and other browning-specific markers, suggesting its possible role in augmentation of lipolysis and suppression of lipogenesis by trans-differentiation from white adipocytes into brown adipocytes (beige).
authors.affiliation: Catholic Kwandong University, International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea. kimsw@daegu.ac.kr.|Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 210-701, Republic of Korea. kimsw@daegu.ac.kr.
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, 712-714, Republic of Korea.
authors.forename: Jae Heon
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, 712-714, Republic of Korea.
authors.forename: Rajib
authors.initials: R
authors.lastname: Mukherjee
authors.affiliation: Catholic Kwandong University, International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.|Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 210-701, Republic of Korea.
authors.forename: Ki-Chul
authors.initials: KC
authors.lastname: Hwang
authors.affiliation: Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, 712-714, Republic of Korea. jwyun@daegu.ac.kr.
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Yun
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D003474:Curcumin
keywords: Anti-obesity; Curcumin; Fat-browning; Proteome; White adipocytes