title: Murine peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived NK cells.
abstract: Murine NK cells can be divided by the expression of two cell surface markers, CD27 and Mac-1 (a.k.a. CD11b), into four separate subsets. These subsets suggest a linear development model: CD27(-) Mac-1(-) → CD27(+) Mac-1(-) → CD27(+) Mac-1(+) → CD27(-) Mac-1(+) . Here, we used a combination of BrdU labeling experiments and mathematical modeling to gain insights regarding NK-cell development in mouse bone marrow (BM), spleen and liver. The modeling results that best fit the experimental data show that the majority of NK cells already express CD27 upon entering the NK-cell developmental pathway. Additionally, only a small fraction of NK cells exit the BM to other sites, suggesting that peripheral NK-cell populations originate from site-specific immature NK cells more than from BM-derived mature NK cells.
authors.affiliation: The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Nissim
authors.initials: N
authors.lastname: Pinhas
authors.affiliation: The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Sternberg-Simon
authors.affiliation: Giannina Gaslini Institute, Genova, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Chiossone
authors.affiliation: The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Gitit
authors.initials: G
authors.lastname: Shahaf
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI), INSERM-CNRS, Lyon, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Walzer
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University UM2, Inserm U1104, CNRS UMR7280, Marseille, France.|Service d'Immunologie, Assistance Publique-Hôpitaux de Marseille, Marseille, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Vivier
authors.affiliation: The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Ramit
authors.initials: R
authors.lastname: Mehr
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology* / Q000502:physiology; D039481:CD11b Antigen / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003198:Computer Simulation; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D008099:Liver / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008962:Models, Theoretical; D013154:Spleen / Q000276:immunology* / Q000502:physiology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology / Q000378:metabolism
chemical_list: D039481:CD11b Antigen; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
keywords: CD11b; CD27; Computer simulation; Mathematical model; Natural killer cells