title: Analysis of differential β variable region of T cell receptor expression and NAV3/TNFRSF1B gene mutation in mycosis fungoides.
abstract: OBJECTIVE
  This study aimed to analyze the predominant expression of the variable region of T cell receptor (TRBV) and determine whether NAV3 or TNFRSF1B gene mutation involved in the pathogenesis of MF.
  
  
  RESULTS
  TRBV5-7 expression increased from the normal, early-stage to advanced-stage lesion in MF patient. By contrast, TRBV2 decreased with the lesion developed. We found no mutations of NAV3 or TNFRSF1B in the lesions from this study.
  
  
  METHODS
  Real-time PCR were used to screen differential expression of TRBV in different lesions. Mutational analyses were used to validate genetic alterations in the skin lesions.
  
  
  CONCLUSIONS
  The identification of TRBV gene expression differences between normal and different stages of MF lesions provide insight into promising new diagnostic and prognostic biomarkers. None of the reported genetic abnormalities suggests complexity of progress from a primary cytogenetic event to an advanced stage with poor prognosis in MF.
authors.affiliation: Department of Dermatology, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.
authors.forename: Hongzhou
authors.initials: H
authors.lastname: Cui
authors.affiliation: Jiangxi Provincial Center for Disease Control and Prevention, Nanchang, Jiangxi 330029, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Dermatology, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Dermatology, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Dermatology, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Dermatology, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi 030001, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Bai
mesh_terms: D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009154:Mutation; D009182:Mycosis Fungoides / Q000235:genetics* / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis* / Q000235:genetics; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000235:genetics* / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; C464453:NAV3 protein, human; D009419:Nerve Tissue Proteins; D011948:Receptors, Antigen, T-Cell; D047889:Receptors, Tumor Necrosis Factor, Type II; C415902:TNFRSF1B protein, human
keywords: NAV3; T cell receptor beta; TNFRSF1B; mycosis fungoides