title: Extraction and purification of a highly thermostable alkaline caseinolytic protease from wastes Penaeus vannamei suitable for food and detergent industries.
abstract: A novel thermostable protease was purified from Penaeus vannamei from Persian Gulf to homogeneity level using ammonium sulfate precipitation and anion-exchange chromatography. The purified protease showed a single band on native and SDS-PAGE with a molecular weight of 24kDa on SDS-PAGE. The enzyme showed the broad highest catalytic activity for hydrolysis of the substrate with maximal activity at pH 7 and 80°C. Activity of the enzyme was inhibited by Hg(2+), Zn(2+) Co(2+) and Cu(2+), while protease activity was increased in the presence of Fe(2+) and Mn(2+) by factors of 173% and 102%, respectively. Enzyme shows a broad substrate specificity and hydrolyzes both natural and synthetic substrates. Based on the Michaelis-Menten plots, the Km with casein as substrate was 16.8μM and Vmax was 82.6μM/min. The enzyme, derived from L. vannamei, possesses unique characteristics and could be used in various industrial and biotechnological applications.
authors.affiliation: Department of Marine Biology, Faculty of Sciences, Hormozgan University, Bandar Abbas, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Dadshahi
authors.affiliation: Department of Biochemistry, Faculty of Sciences, Hormozgan University, Bandar Abbas, Iran. Electronic address: a.homaei@hormozgan.ac.ir.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Homaei
authors.affiliation: Department of Marine Biology, Faculty of Sciences, Hormozgan University, Bandar Abbas, Iran.
authors.forename: Farrokhzad
authors.initials: F
authors.lastname: Zeinali
authors.affiliation: Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Reza H
authors.initials: RH
authors.lastname: Sajedi
authors.affiliation: Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Khosro
authors.initials: K
authors.lastname: Khajeh
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002364:Caseins / Q000378:metabolism*; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D033561:Penaeidae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D002364:Caseins; D010450:Endopeptidases; C087822:alkaline protease
keywords: Marine wastes; Penaeus vannamei protease; Persian Gulf; Purification; Thermostable