title: Nutritional effects on the mycelial growth and enzymatic activity of Isaria farinosa, and Hepialus larvae growth.
abstract: OBJECTIVE
  To investigate the nutritional requirements of the mycelial growth and pathogenesis-related enzymatic activity on Isaria farinosa and the nutritional effect of fungus on its host Hepialus larvae.
  
  
  RESULTS
  Sixteen carbon sources, 16 nitrogen sources and 9 vitamin combinations were tested. The nutritional conditions that significantly prevented I. farinosa pathogenesis were selected as feed additives for rearing Hepialus larvae. Citric acid significantly inhibited the growth of I. farinosa and the activity of three enzymes. l-histidine and l-aspartic acid significantly reduced the dry weights of mycelia and their protease and lipase activities. Vitamin combination that lacked VB 1 significantly increased the growth of I. farinosa and enhanced its chitinase and lipase activities. l-aspartic acid, VB 1 or a combination of them were beneficial for maintaining the larvae survival rate and decreasing the disease rate. The result provides new insight to develop a nutrition-based strategy to control fungal epidemics during insect rearing.
  
  
  CONCLUSIONS
  The ability of some specific nutrients to inhibit mycelial growth and enzymatic activity can prevent epidemics of fungal disease.
  
  
  CONCLUSIONS
  These results will aid in the development of nutrition-based strategies to control entomopathogenic fungal epidemics during the large-scale rearing of insects.
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing, China.|Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing, China.|Chongqing Sub-center of the National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing, China.|Ganzi District Kangding Gongga Chinese Caterpillar Fungus Co. Ltd., Sichuan, China.
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing, China.|Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing, China.|Chongqing Sub-center of the National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Wu
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing, China.|Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing, China.|Chongqing Sub-center of the National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing, China.|Ganzi District Kangding Gongga Chinese Caterpillar Fungus Co. Ltd., Sichuan, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: He
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Science, Beijing, China.
authors.forename: M C
authors.initials: MC
authors.lastname: Xiang
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing, China.|Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing, China.|Chongqing Sub-center of the National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing, China.|Ganzi District Kangding Gongga Chinese Caterpillar Fungus Co. Ltd., Sichuan, China.
authors.forename: Y C
authors.initials: YC
authors.lastname: He
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Science, Beijing, China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Liu
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing, China.|Chongqing Key Laboratory of Chinese Medicine Resources, Chongqing, China.|Chongqing Sub-center of the National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing, China.|Ganzi District Kangding Gongga Chinese Caterpillar Fungus Co. Ltd., Sichuan, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: Ganzi District Kangding Gongga Chinese Caterpillar Fungus Co. Ltd., Sichuan, China.
authors.forename: W
authors.initials: W
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002244:Carbon / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D006999:Hypocreales / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D007814:Larva / Q000254:growth & development* / Q000382:microbiology; D008049:Lipase / Q000378:metabolism; D009036:Moths / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D025282:Mycelium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D006639:Histidine; D002244:Carbon; D008049:Lipase; D002688:Chitinases; D010447:Peptide Hydrolases; D009584:Nitrogen
keywords: Hepialus; Isaria farinosa; Ophiocordyceps sinensis; enzyme activity; nutrition