title: Development of a multiplex PCR assay to detect the major clonal complexes of Streptococcus suis relevant to human infection.
abstract: Multilocus sequence typing (MLST) is considered a reliable method for providing insight into the Streptococcus suis population structure, clonal relationships and the potential of particular clones to cause disease. Indeed, MLST has revealed the presence of several clonal complexes (CCs) within the Streptococcus suis population. However, the method is costly, time-consuming and difficult to use for screening large numbers of isolates. In this study, a multiplex PCR assay was developed to identify Streptococcus suis CCs that are relevant to human infections. The multiplex PCR assay was capable of simultaneously distinguishing CC1, CC25, CC28, CC104, CC221/234 and CC233/379, which are related to human infections in Thailand, in a single reaction. The multiplex PCR assay is useful for low-cost screening of large numbers of isolates with rapid analytical capacity and could be utilized in most laboratories.
authors.affiliation: Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus,Sakon Nakhon,Thailand.
authors.forename: Rujirat
authors.initials: R
authors.lastname: Hatrongjit
authors.affiliation: Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus,Sakon Nakhon,Thailand.
authors.forename: Anusak
authors.initials: A
authors.lastname: Kerdsin
authors.affiliation: Faculty of Veterinary Medicine, University of Montreal,Québec,Canada.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.affiliation: Thailand-Japan Research Collaboration Center for Emerging and Re-emerging Infections, Research Institute for Microbial Diseases,Osaka University, Osaka,Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Hamada
authors.affiliation: Infectious Disease Surveillance Center, National Institute of Infectious Diseases,Tokyo,Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Oishi
authors.affiliation: Research Institute for Microbial Diseases, Osaka University,Osaka,Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akeda
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D013290:Streptococcal Infections / Q000382:microbiology*; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial