title: In-Situ-Generated Vasoactive Intestinal Peptide Loaded Microspheres in Mussel-Inspired Polycaprolactone Nanosheets Creating Spatiotemporal Releasing Microenvironment to Promote Wound Healing and Angiogenesis.
abstract: Vasoactive intestinal peptide (VIP) was reported to promote angiogenesis. Electrospun nanofibers lead to idea wound dressing substrates. Here we report a convenient and novel method to produce VIP loaded microspheres in polycaprolactone (PCL) nanofibrous membrane without complicated processes. We first coated mussel-inspired dopamine (DA) to nanofibers, then used strong adhesive DA to absorb the functional peptide. PCL membrane was then immersed into acetone to generate microspheres with VIP loading. We employed high pressure liquid chromatography to record encapsulation efficiency of (31.8 ± 2.2)% and loading capacity of (1.71 ± 0.16)%. The release profile of VIP from nanosheets showed a prolonged release. The results of laser scanning confocal microscope, scanning electron microscope and cell counting kit-8 proliferation assays showed that cell adhesion and proliferation were promoted. In order to verify the efficacy on wound healing, in vivo implantation was applied in the full-thickness defect wounds of BALB/c mice. Results showed that the wound healing was significantly promoted via favoring the growth of granulation tissue and angiogenesis. However, we found wound re-epithelialization was not significantly improved. The resulting VIP-DA-coated PCL (PCL-DA-VIP) nanosheets with spatiotemporal delivery of VIP could be a potential application in wound treatment and vascular tissue engineering.
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Mechanical Engineering, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.|Department of Biochemistry and Medical Genetics, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.|Children's Hospital Research Institute of Manitoba , Winnipeg, Manitoba R3E 3P4, Canada.
authors.forename: Kaige
authors.initials: K
authors.lastname: Xu
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lei
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Tan
authors.affiliation: Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University , Chongqing 400038, China.|Chongqing Key Laboratory for Disease Proteomics , Chongqing 400038, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Mechanical Engineering, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.|Department of Biochemistry and Medical Genetics, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.|Children's Hospital Research Institute of Manitoba , Winnipeg, Manitoba R3E 3P4, Canada.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Xing
mesh_terms: D000818:Animals; D049872:Bivalvia; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004298:Dopamine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D057139:Nanofibers / Q000737:chemistry*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011091:Polyesters / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D003692:Delayed-Action Preparations; D011091:Polyesters; C016240:polycaprolactone; D014660:Vasoactive Intestinal Peptide; D004298:Dopamine
keywords: angiogenesis; controlled release of vasoactive intestinal peptide; in situ generated microspheres; mussel-inspired dopamine; wound healing