title: [Simultaneous Determination of Seven Chemical Markers and Preliminary Identification of Chemical Constituents in Daodi Psoraleae Fructus-Myristicae Semen Chinese Drug Pair].
abstract: OBJECTIVE
  To study the simultaneous determination method of daodi Psoraleae Fructus-Myristicae Semen Chinese drug pair for the seven ingredients, and Psoraleae Fructus-Myristicae Semen Chinese drug pair on the chemical composition of initial ownership and identification.
  
  
  METHODS
  UPLC BEH C18 column (2.1 mm x 100 mm, 1.7 µm) was used in the determination. The flow rate was kept at 0.25 mL/min, and 2 µL of standard and sample solution were injected in each run. The mobile phase was consisted of acetonitrile and water using a gradient elution. The UPLC/Q-TOF MS condition: Waters HSS T3 (100 mm x 2.1 mm,1.7 µm); capillary voltage 3.0 kV (positive ion mode) and 2.5 kV (negative ion mode); Mass spectrometric detection was carried out on a Waters Xevo G2 Q/ TOF mass spectrometer equipped with an ESI source operating in both positive and negative ion modes. The parameters of the mass spectrometer under the ESI mode were as follows: ion source temperature 110 °C, cone gas flow 50 L/h, desolvation gas temperature 450 °C, desolvation gas flow 800 L/h.
  
  
  RESULTS
  The seven chemical markers in the selected linear range had good linearity. The recoveries were in the range of 95.07%-98.16% and RSDs were between 1.23%-1.97%.
  
  
  CONCLUSIONS
  It is suitable for the quality control and further studies of the herb in vivo of daodi Psoraleae Fructus-Myristicae Semen Chinese drug pair.
authors.forename: Jia-rong
authors.initials: JR
authors.lastname: Gao
authors.forename: Xiao-xu
authors.initials: XX
authors.lastname: Yang
authors.forename: Xi
authors.initials: X
authors.lastname: Wu
authors.forename: Liang-bing
authors.initials: LB
authors.lastname: Wei
authors.forename: Yan-quan
authors.initials: YQ
authors.lastname: Han
authors.forename: Jun-mei
authors.initials: JM
authors.lastname: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry; D026324:Myristicaceae / Q000737:chemistry*; D029907:Psoralea / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal