title: Nucleoplasmic Lamin A/C and Polycomb group of proteins: An evolutionarily conserved interplay.
abstract: Nuclear lamins are the main components of the nuclear lamina at the nuclear periphery, providing mechanical support to the nucleus. However, recent findings suggest that lamins also reside in the nuclear interior, as a distinct and dynamic pool with critical roles in transcriptional regulation. In our work we found a functional and evolutionary conserved crosstalk between Lamin A/C and the Polycomb group (PcG) of proteins, this being required for the maintenance of the PcG repressive functions. Indeed, Lamin A/C knock-down causes PcG foci dispersion and defects in PcG-mediated higher order structures, thereby leading to impaired PcG mediated transcriptional repression. By using ad-hoc algorithms for image analysis and PLA approaches we hereby show that PcG proteins are preferentially located in the nuclear interior where they interact with nucleoplasmic Lamin A/C. Taken together, our findings suggest that nuclear components, such as Lamin A/C, functionally interact with epigenetic factors to ensure the correct transcriptional program maintenance.
authors.affiliation: a CNR Institute of Cell Biology and Neurobiology, IRCCS Santa Lucia Foundation , Rome , Italy.
authors.forename: F
authors.initials: F
authors.lastname: Marullo
authors.affiliation: a CNR Institute of Cell Biology and Neurobiology, IRCCS Santa Lucia Foundation , Rome , Italy.
authors.forename: E
authors.initials: E
authors.lastname: Cesarini
authors.affiliation: b CNR Institute for High Performance Computing and Networking (ICAR) , Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Antonelli
authors.affiliation: b CNR Institute for High Performance Computing and Networking (ICAR) , Naples, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gregoretti
authors.affiliation: b CNR Institute for High Performance Computing and Networking (ICAR) , Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Oliva
authors.affiliation: a CNR Institute of Cell Biology and Neurobiology, IRCCS Santa Lucia Foundation , Rome , Italy.|c Istituto Nazionale Genetica Molecolare Romeo ed Enrica Invernizzi , Milan , Italy.
authors.forename: C
authors.initials: C
authors.lastname: Lanzuolo
mesh_terms: D021581:Active Transport, Cell Nucleus; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D019143:Evolution, Molecular; D057809:HEK293 Cells; D006801:Humans; D034904:Lamin Type A / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology; D063146:Polycomb-Group Proteins / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D034904:Lamin Type A; D063146:Polycomb-Group Proteins
keywords: Lamin A/C; Polycomb; nuclear architecture; nucleoplasm; transcription