title: The effectiveness of chemotherapy and electrochemotherapy on ovarian cell lines in vitro.
abstract: The presented study aimed to evaluate in vitro the effectiveness of improvement standard chemotherapy with bleomycin by electroporation in two various ovarian cancer cell lines. Two human ovarian cell lines OvBH-1 and SKOV-3 were used. The lines were selected because of their resistance to several therapeutic methods. As anticancer drug we use range of concentrations of bleomycin. In EP and ECT experiments different voltage values: from 0 to 1200 V/cm, 8 pulses with duration of 100μs and intervals between pulses 1s long were used. The cells viability after applied treatments was evaluated by MTT assay. The expression of heat shock proteins - HSP27 was examined by immunocytochemical ABC method.The cytotoxicity with different concentrations of bleomycin alone was not significantly decrease in both cell lines. It confirms resistance of these cells to conventional chemotherapy. The highest decrease of cell proliferation was observed after EP with bleomycin after 48h of incubation for 1000 V/cm. The intensity of expression of small heat shock proteins HSP27 slightly increased after ECT in both treated cell lines, in particular in OvBH-1. The presented study indicated that application of electroporation may effectively enhance chemotherapy with bleomycin, particularly in the case of treating ovarian cancer resistant to standard therapy.
authors.forename: J
authors.initials: J
authors.lastname: Saczko
authors.forename: J
authors.initials: J
authors.lastname: Pilat
authors.forename: A
authors.initials: A
authors.lastname: Choromanska
authors.forename: N
authors.initials: N
authors.lastname: Rembialkowska
authors.forename: J
authors.initials: J
authors.lastname: Bar
authors.forename: I
authors.initials: I
authors.lastname: Kaminska
authors.forename: J
authors.initials: J
authors.lastname: Zalewski
authors.forename: J
authors.initials: J
authors.lastname: Kulbacka
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D001761:Bleomycin / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053672:Electrochemotherapy / Q000379:methods*; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D018832:Molecular Chaperones; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000903:Antibiotics, Antineoplastic; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D001761:Bleomycin
keywords: bleomycin; chemotherapy; electrochemotherapy; electroporation; ovarian cancer.