title: Phenolic Compounds in Chilean Mistletoe (Quintral, Tristerix tetrandus) Analyzed by UHPLC-Q/Orbitrap/MS/MS and Its Antioxidant Properties.
abstract: Mass spectrometry has become a method of choice to characterize bioactive compounds in biological samples because of its sensitivity and selectivity. Hybrid ultra-HPLC hyphenated with Orbitrap mass analyzer is an innovative state of the art technology that allows fast and accurate metabolomic analyses. In this work the metabolites of a Chilean mistletoe endemic to the VIII region of Chile were investigated for the first time using UHPLC mass analysis (UHPLC-PDA-HESI-Orbitrap MS(n)). The anthocyanins, together with the non-pigmented phenolics were fingerprinted and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Six anthocyanins were identified, and among them, the 3-O-glycosides of delphinidin and cyanidin were the major ones. In addition, several phenolic acids (including feruloylquinic acid, feruloyl glucose, chlorogenic acid) and several flavonols (luteolin, quercetin, apigenin, isorhamnetin and glycoside derivatives) were also identified. The mistletoe leaves showed the highest antioxidant activity as measured by the DPPH radical bleaching, ferric reducing antioxidant power and superoxide anion scavenging activity tests (13.38 ± 0.47 µg/mL, 125.32 ± 5.96 µmolTE/g DW and 84.06 ± 4.59 at 100 µg/mL, respectively).
authors.affiliation: Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta 1240000, Chile. mario.simirgiotis@uantof.cl.
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Simirgiotis
authors.affiliation: Facultad de Ciencias de la Salud, Universidad Arturo Prat, Casilla 121, Iquique 1110939, Chile. elquispe@unap.cl.
authors.forename: Cristina
authors.initials: C
authors.lastname: Quispe
authors.affiliation: Departamento de Química, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago 7800024, Chile. areche@uchile.cl.
authors.forename: Carlos
authors.initials: C
authors.lastname: Areche
authors.affiliation: Departamento de Ciencias Químicas, Universidad Andres Bello, Campus Viña del Mar, Quillota 980, Viña del Mar 2520000, Chile. bsepulveda@uc.cl.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sepúlveda
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002677:Chile; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D044948:Flavonols / Q000737:chemistry* / Q000302:isolation & purification; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000302:isolation & purification; D014784:Mistletoe / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D044948:Flavonols; D062385:Hydroxybenzoates; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl; C017616:phenolic acid
keywords: anthocyanins; antioxidants; flavonoids; muérdago; phenolic acids; quintral del álamo; ultra HPLC-MS