title: Characterization of an Arxula adeninivorans alcohol dehydrogenase involved in the metabolism of ethanol and 1-butanol.
abstract: In this study, alcohol dehydrogenase 1 from Arxula adeninivorans (Aadh1p) was identified and characterized. Aadh1p showed activity with short and medium chain length primary alcohols in the forward reaction and their aldehydes in the reverse reaction. Aadh1p has 64% identity with Saccharomyces cerevisiae Adh1p, is localized in the cytoplasm and uses NAD(+) as cofactor. Gene expression analysis showed a low level increase in AADH1 gene expression with ethanol, pyruvate or xylose as the carbon source. Deletion of the AADH1 gene affects growth of the cells with 1-butanol, ethanol and glucose as the carbon source, and a strain which overexpressed the AADH1 gene metabolized 1-butanol more rapidly. An ADH activity assay indicated that Aadh1p is a major enzyme for the synthesis of ethanol and the degradation of 1-butanol in A. adeninivorans.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Correnstr. 3, D-06466 Gatersleben, Germany.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kasprzak
authors.affiliation: Orgentis Chemicals GmbH, Bahnhofstr. 3-5, D-06466 Gatersleben, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Rauter
authors.affiliation: Jäckering Mühlen- und Nährmittelwerke GmbH, Vorsterhauser Weg 46, D-59007 Hamm, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Riechen
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Correnstr. 3, D-06466 Gatersleben, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Worch
authors.affiliation: School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
authors.forename: Kim
authors.initials: K
authors.lastname: Baronian
authors.affiliation: Institute of Microbiology, University of Greifswald, Jahnstr. 15, D-17487 Greifswald, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Bode
authors.affiliation: Institute of Microbiology, University of Greifswald, Jahnstr. 15, D-17487 Greifswald, Germany.
authors.forename: Frieder
authors.initials: F
authors.lastname: Schauer
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Correnstr. 3, D-06466 Gatersleben, Germany kunzeg@ipk-gatersleben.de.
authors.forename: Gotthard
authors.initials: G
authors.lastname: Kunze
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002244:Carbon / Q000378:metabolism; D003067:Coenzymes / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014994:Xylose / Q000378:metabolism
chemical_list: D003067:Coenzymes; D009243:NAD; D000431:Ethanol; D002244:Carbon; D019289:Pyruvic Acid; D020001:1-Butanol; D014994:Xylose; D000426:Alcohol Dehydrogenase
keywords: 1-butanol; Arxula adeninivorans; alcohol dehydrogenase; ethanol; metabolism; yeast