title: Investigation of enrofloxacin residues in broiler tissues using ELISA and LC-MS/MS.
abstract: This study investigated the efficiency of an enrofloxacin ELISA test kit to detect the presence of enrofloxacin residues in broiler tissues compared with LC-MS/MS. Broiler tissues from 72 samples consisting of 60 breast muscle, six pools of livers (500 g each) and six pools of kidneys (500 g each) were obtained from six different slaughterhouses. Breast muscle from 10 carcasses and pools of livers and kidneys from approximately 200 carcasses of the same flock were collected from each slaughterhouse. ELISA and HPLC were used to identify and quantify the contamination of the samples with enrofloxacin. A total of 72% of the analysed samples contained enrofloxacin residues detected by the ELISA and 22.2% were detected by LC-MS/MS. The mean values of enrofloxacin contamination found in chicken breast by ELISA and HPLC were 8.63 and 12.25 μg kg(-1), respectively. None of the samples exceeded the maximum limit of 100 μg kg(-1) by both methods set by the European Union as well as the Brazilian Agriculture Ministry. All positive samples for enrofloxacin residues detected by LC-MS/MS were also positive by ELISA. These data confirm the efficiency of the ELISA test, and suggest its use as a screening method for enrofloxacin residues in poultry tissues due to its quick results, low price and ease of applicability.
authors.affiliation: a Department of Veterinary Public Health and Public Health , Federal Fluminense University (UFF) , Niterói , Brazil.
authors.forename: Pedro Henrique N
authors.initials: PH
authors.lastname: Panzenhagen
authors.affiliation: a Department of Veterinary Public Health and Public Health , Federal Fluminense University (UFF) , Niterói , Brazil.
authors.forename: Waldemir S
authors.initials: WS
authors.lastname: Aguiar
authors.affiliation: a Department of Veterinary Public Health and Public Health , Federal Fluminense University (UFF) , Niterói , Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Gouvêa
authors.affiliation: b Ministry of Agriculture, Livestock and Food Supply , MAPA, National Agricultural Laboratory, LANAGRO/MG/RS , Pedro Leopoldo , Brazil.
authors.forename: Andréa M G
authors.initials: AM
authors.lastname: de Oliveira
authors.affiliation: b Ministry of Agriculture, Livestock and Food Supply , MAPA, National Agricultural Laboratory, LANAGRO/MG/RS , Pedro Leopoldo , Brazil.
authors.forename: Fabiano
authors.initials: F
authors.lastname: Barreto
authors.affiliation: a Department of Veterinary Public Health and Public Health , Federal Fluminense University (UFF) , Niterói , Brazil.
authors.forename: Virgínia L A
authors.initials: VL
authors.lastname: Pereira
authors.affiliation: a Department of Veterinary Public Health and Public Health , Federal Fluminense University (UFF) , Niterói , Brazil.
authors.forename: Maria Helena C
authors.initials: MH
authors.lastname: Aquino
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D000077422:Enrofloxacin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis*; D011202:Poultry Products / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin
keywords: ELISA; Enrofloxacin residues; HPLC; chicken carcasses; chicken liver