title: Extraction of polysaccharides and its antitumor activity on Magnolia kwangsiensis Figlar & Noot.
abstract: The crude polysaccharides from the leaves of Magnolia kwangsiensis Figlar & Noot. were extracted by hot water extraction, the yield was 5.09%. Two polysaccharide fractions (P-2 and P-3) were isolated by DEAE-52 cellulose chromatography and Sephadex G-100 column chromatography in order, respectively. P-2 and P-3 were characterized by IR, HPGPC and GC-MS. P-2 was comprised of only glucose. Its molecular weight was 11.2×10(3) Da and the formula was C414H690O345. P-3 was comprised of xylose and rhamnose in the ratio of 1:4. Its molecular weight was 7.8×10(3)Da, and the formula was C319H528O220. The antitumor activities of P-2 and P-3 on the growth of human lung cancer (A549) cells and human gastric carcinoma (SGC7901) cells in vitro were evaluated. The results indicated that P-3 exhibited marked antitumor activities with IC50 value of 8.48 and 5.66 μg/mL.
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China. Electronic address: zhengyanfei071@163.com.
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Zheng
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China. Electronic address: wwwbxp@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China. Electronic address: xmliu1@gxu.edu.cn.
authors.forename: Xiong-Min
authors.initials: XM
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China. Electronic address: gxumali@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, China. Electronic address: laifang200609@126.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Lai
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D031566:Magnolia / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011134:Polysaccharides
keywords: Antitumor; Extraction; Magnolia kwangsiensis Figlar & Noot.; Polysaccharides