title: [Therapeutic Material Basis of Chemical Subdivisions of Anemarrhenae Rhizoma on Anti-inflammatory and Immunomudulatory Effects].
abstract: OBJECTIVE
  To study the relationship between chemical subdivision and immune suppressive activity in order to find out the anti-inflammatory and immunomudulatory pharmacological activity material basic of Anemarrhenae Rhizoma.
  
  
  METHODS
  C57 mice was induced by 2,4-Dinitrofluorobenzene to build immune inflammation which was also called contact allergic dermatitis. The influence of Anemarrhenae Rhizoma decoction and chemical subdivisions on immune organ, ear edema and the Th1/Th2 immune balance was measured by analytical balance and Elisa method. The inflammatory factor TNF-α and NO level excreted by macrophage RAW264. 7 induced by LPS were also investigated.
  
  
  RESULTS
  AA decoction, timosaponin and polysaccharides significantly reduced the immune organ index and ear edema degree (P < 0.05), protein expression of IFN-γ was inhibited by AA timosaponin fraction and polysaccharides fraction. In vitro experiments showed that the proliferation of spleen cells was inhibited by timosaponin and polysaccharides after induced by ConA (P < 0.05). The release of NO and TNF-α induced by LPS significantly decreased by Anemarrhenae Rhizoma decoction and timosaponin (P < 0.05).
  
  
  CONCLUSIONS
  The significant anti-inflammatory and immunomudu latory effects of AA are related to timosaponin and polysaccharides.
authors.forename: Xia
authors.initials: X
authors.lastname: Lei
authors.forename: Wen-ting
authors.initials: WT
authors.lastname: Dong
authors.forename: Xue-yan
authors.initials: XY
authors.lastname: Bi
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Su
authors.forename: Yong-gang
authors.initials: YG
authors.lastname: Xia
authors.forename: Qiu-hong
authors.initials: QH
authors.lastname: Wang
authors.forename: Hai-xue
authors.initials: HX
authors.lastname: Kuang
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004139:Dinitrofluorobenzene; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000276:immunology; D064209:Phytochemicals / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology; D000067996:RAW 264.7 Cells; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects; D058408:Th1-Th2 Balance; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D064209:Phytochemicals; D011134:Polysaccharides; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D004139:Dinitrofluorobenzene