title: A Structural Perspective on Readout of Epigenetic Histone and DNA Methylation Marks.
abstract: This article outlines the protein modules that target methylated lysine histone marks and 5mC DNA marks, and the molecular principles underlying recognition. The article focuses on the structural basis underlying readout of isolated marks by single reader molecules, as well as multivalent readout of multiple marks by linked reader cassettes at the histone tail and nucleosome level. Additional topics addressed include the role of histone mimics, cross talk between histone marks, technological developments at the genome-wide level, advances using chemical biology approaches, the linkage between histone and DNA methylation, the role for regulatory lncRNAs, and the promise of chromatin-based therapeutic modalities.
authors.affiliation: Structural Biology Department, Memorial Sloan-Kettering Cancer Center, New York, New York 10065.
authors.forename: Dinshaw J
authors.initials: DJ
authors.lastname: Patel
mesh_terms: D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D018899:CpG Islands; D019175:DNA Methylation; D057890:Epigenomics; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D008745:Methylation; D008957:Models, Genetic; D008958:Models, Molecular; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism; D000072417:Protein Domains; D011499:Protein Processing, Post-Translational; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D018398:Homeodomain Proteins; D009707:Nucleosomes; D062085:RNA, Long Noncoding