title: Effects of Curcumin on Parameters of Myocardial Oxidative Stress and of Mitochondrial Glutathione Turnover in Reoxygenation after 60 Minutes of Hypoxia in Isolated Perfused Working Guinea Pig Hearts.
abstract: In cardiovascular surgery ischemia-reperfusion injury is a challenging problem, which needs medical intervention. We investigated the effects of curcumin on cardiac, myocardial, and mitochondrial parameters in perfused isolated working Guinea pig hearts. After preliminary experiments to establish the model, normoxia was set at 30 minutes, hypoxia was set at 60, and subsequent reoxygenation was set at 30 minutes. Curcumin was applied in the perfusion buffer at 0.25 and 0.5 μM concentrations. Cardiac parameters measured were afterload, coronary and aortic flows, and systolic and diastolic pressure. In the myocardium histopathology and AST in the perfusate indicated cell damage after hypoxia and malondialdehyde (MDA) levels increased to 232.5% of controls during reoxygenation. Curcumin protected partially against reoxygenation injury without statistically significant differences between the two dosages. Mitochondrial MDA was also increased in reoxygenation (165% of controls), whereas glutathione was diminished (35.2%) as well as glutathione reductase (29.3%), which was significantly increased again to 62.0% by 0.05 μM curcumin. Glutathione peroxidase (GPx) was strongly increased in hypoxia and even more in reoxygenation (255% of controls). Curcumin partly counteracted this increase and attenuated GPx activity independently in hypoxia and in reoxygenation, 0.25 μM concentration to 150% and 0.5 μM concentration to 200% of normoxic activity.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Ermita I Ibrahim
authors.initials: EI
authors.lastname: Ilyas
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Busjra M
authors.initials: BM
authors.lastname: Nur
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Sonny P
authors.initials: SP
authors.lastname: Laksono
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Anton
authors.initials: A
authors.lastname: Bahtiar
authors.affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Ari
authors.initials: A
authors.lastname: Estuningtyas
authors.affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Caecilia
authors.initials: C
authors.lastname: Vitasyana
authors.affiliation: National Cardiovascular Center, Harapan Kita Hospital and Department of Cardiology and Vascular Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Dede
authors.initials: D
authors.lastname: Kusmana
authors.affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Frans D
authors.initials: FD
authors.lastname: Suyatna
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Muhammad Kamil
authors.initials: MK
authors.lastname: Tadjudin
authors.affiliation: Medical Research Unit, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Freisleben