title: Nitrogen-doped graphene-chitosan matrix based efficient chemiluminescent immunosensor for detection of chicken interleukin-4.
abstract: Chicken interleukin-4 (ChIL-4), which is released by activated type 2 helper (Th2) cells following their stimulation in vitro, is an important indicator for the study of cell-mediated immunity in chickens after infection or vaccination. In this work, the first ChIL-4 chemiluminescent (CL) immunosensor was developed via the immobilization of monoclonal ChIL-4 antibodies on a nitrogen-doped graphene (NG)-chitosan nanocomposite matrix. NG nanosheets were used for the first time in the CL immunoassay to provide a biocompatible microenvironment for the immobilized capture antibody. The ChIL-4 immunosensor was characterized systematically. The proposed immunosensor displayed a wide linear range from 0.05 to 70ngmL-1and a low detection limit of 0.02ngmL-1at a signal-to-noise ratio of 3. Compared to traditional assay methods, this system was more flexible, simple, rapid, and sensitive. Moreover, this CL immunoassay system had an excellent detection and fabrication reproducibility, a high specificity, an acceptable accuracy, and a high stability. This work enables the specific detection of ChIL-4 and the further study of its role in the immune responses of poultry.
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China. Electronic address: zjyang@yzu.edu.cn.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Mimi
authors.initials: M
authors.lastname: Lu
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Zining
authors.initials: Z
authors.lastname: Tan
authors.affiliation: School of Medicine; Jiangsu Key Lab of Zoonosis, Yangzhou University, Yangzhou 225002, PR China. Electronic address: daihua@yzu.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Dai
authors.affiliation: School of Medicine; Jiangsu Key Lab of Zoonosis, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Xin'an
authors.initials: X
authors.lastname: Jiao
authors.affiliation: School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods; D002645:Chickens; D048271:Chitosan / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000191:economics / Q000379:methods*; D015847:Interleukin-4 / Q000032:analysis*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D015847:Interleukin-4; D006108:Graphite; D048271:Chitosan
keywords: Chemiluminescent; Chicken interleukin-4; Cytokine; Immunoassay; Nitrogen-doped graphene