title: Epidemiological and pathological features of primary cardiac tumours in dogs from Poland in 1970-2014.
abstract: Primary heart tumours affect less than 1% of dogs. Due to their rare incidence, every research showing the frequency of cardiac tumours is valuable. Routine diagnostics is often complemented with immunohistochemical analysis. This study was conducted on 110 patient records from all veterinary faculties in Poland from dogs diagnosed with heart tumours between 1970 and 2014. The dogs' age, breed and sex with tumour localisation and histopathological diagnosis were analysed. Because of its most common incidence, samples of haemangiosarcoma underwent further examination with assessment of the expression of cell markers that have not been evaluated earlier (i.e. minichromosome maintenance proteins and beta-catenin). We noted 111 tumours including 88.3% malignant and 10.8% benign ones. Haemangiosarcoma and aortic body tumour were the most frequent cardiac neoplasms in the dogs examined (45.9% and 27.9% of all tumours, respectively). Immunohistochemical analysis of haemangiosarcoma showed a positive expression of all markers examined. CD31, vimentin, and beta-catenin showed a positive reaction in all 11 samples examined. At least one proliferative marker (Ki-67, MCM-3 or MCM-7) showed a positive reaction in each sample. MCM-3 showed a higher expression than the two other proliferative markers (P = 0.006), but only Ki-67 showed a positive correlation with the mitotic index (P > 0.05, r = 0.89). Although beta-catenin, MCM-3 and MCM-7 showed a positive reaction in the haemangiosarcomas examined, their usefulness as diagnostic and prognostic factors should be a topic of further research.
authors.affiliation: Department of Pathology, Wroclaw University of Environmental and Life Sciences , C. K. Norwida 31, 50375 Wroclaw , Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Janus
authors.affiliation: Department of Pathology, Wroclaw University of Environmental and Life Sciences , C. K. Norwida 31, 50375 Wroclaw , Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Nowak
authors.affiliation: Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences , C. K. Norwida 31, 50375 Wroclaw , Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Noszczyk-Nowak
authors.affiliation: Department of Pathology, Wroclaw University of Environmental and Life Sciences , C. K. Norwida 31, 50375 Wroclaw , Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Ciaputa
authors.affiliation: Department of Pathology, Wroclaw University of Environmental and Life Sciences , C. K. Norwida 31, 50375 Wroclaw , Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kandefer-Gola
authors.affiliation: Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences , C. K. Norwida 31, 50375 Wroclaw , Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Pasławska
authors.affiliation: Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine Warsaw University of Life Sciences (SGGW) , Warsaw , Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Sapierzyński
authors.affiliation: Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Life Sciences , Lublin , Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Łopuszyński
authors.affiliation: Department of Pathological Anatomy, Warmia and Mazury University in Olsztyn , Olsztyn , Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Otrocka-Domagała
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D004283:Dog Diseases / Q000453:epidemiology* / Q000473:pathology*; D004285:Dogs; D005260:Female; D006338:Heart Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D011044:Poland / Q000453:epidemiology
chemical_list: D014408:Biomarkers, Tumor
keywords: Cardiac tumour; dog; haemangiosarcoma; immunohistochemistry