title: Ion-pair assisted extraction followed by (1)H NMR determination of biogenic amines in food and biological matrices.
abstract: A selective method for the extraction and determination of six biogenic amines (BAs) by NMR is presented. Briefly, BAs are extracted into an organic solvent via the use of an ion pairing agent, followed by a back extraction in D2O in order to acquire the (1)H NMR spectra. The method is studied with respect to the critical experimental parameters and is successfully applied to selected food substrates (dark chocolate, banana, gouda cheese) and biological samples (urine and blood plasma) signifying its potential as an alternative tool for BAs determination. Accurate and precise results are consistently achieved with all matrixes studied. The calculated limits of detection and limits of quantitation were found to be in the ranges 0.05-0.13μg/mL and 0.14-0.38μg/mL, respectively, for biological samples while for food samples they were in the ranges 2.25-6.25μg/g and 6.75-18.7μg/g, respectively.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece.
authors.forename: T
authors.initials: T
authors.lastname: Chatzimitakos
authors.affiliation: NMR Centre, University of Ioannina, 45110 Ioannina, Greece. Electronic address: vexarc@cc.uoi.gr.
authors.forename: V
authors.initials: V
authors.lastname: Exarchou
authors.affiliation: Laboratory of Food Chemistry & Technology, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: S A
authors.initials: SA
authors.lastname: Ordoudi
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece.
authors.forename: Y
authors.initials: Y
authors.lastname: Fiamegos
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Stalikas
mesh_terms: D001679:Biogenic Amines / Q000032:analysis* / Q000302:isolation & purification; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
chemical_list: D001679:Biogenic Amines
keywords: (1)H NMR spectroscopy; Biogenic amines; Ion-pair extraction