title: Expression of the energy substrate transporters in uterine fibroids.
abstract: Proliferating cells exhibit accelerated rates of substrate utilization, favoring glucose over fatty acids (FA's) oxidation. Protein-mediated transport is thought to play a predominant role in facilitating either glucose or FA routing into the cells. In the present study, we examined the expression of glucose transporters (GLUT-1, GLUT-4) and fatty acids transporters (FAT/CD36, FATP-1, FATP-4) at transcript and protein levels as well as cytosolic fatty acid binding proteins (H-FABP, ACBP) in human fibroids (n=74, size up to 3cm diameter) and compared with pair-matched healthy myometrium. Additionally lipid content (diacylglycerols, triacylglycerols and ceramide) was estimated by gas liquid chromatography (GLC). Uterine fibroids displayed decreased expression of both FAT/CD36 and FATP-1 proteins along with lower diacylglycerol (DAG) and triacylglycerol (TAG) content as compared to healthy pair-matched myometrium. The expression of glucose transport proteins (GLUT-4 and GLUT-1) remained relatively constant, although the higher expression of GLUT-1 in uterine fibroids did not reach the minimum significance threshold (p=0.056). However, no change in either cytochrome c oxidase (COX IV) or hydroxyacyl-CoA dehydrogenase (HADHSC) was observed and these data confirm a possible metabolic shift favoring glucose utilization over fatty acid oxidation in human uterine fibroids.
authors.affiliation: Department of Gynecology and Gynecologic Oncology, Medical University of Bialystok, 24a Sklodowskiej-Curie Str., 15-269 Bialystok, Poland. Electronic address: knapp@umb.edu.pl.
authors.forename: Paweł
authors.initials: P
authors.lastname: Knapp
authors.affiliation: Department of Physiology, Medical University of Bialystok, 2c Mickiewicza Str., 15-222 Bialystok, Poland.
authors.forename: Adrian
authors.initials: A
authors.lastname: Chabowski
authors.affiliation: Department of Perinatology, Medical University of Bialystok, 24a Sklodowskiej-Curie Str., 15-269 Bialystok, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Posmyk
authors.affiliation: Department of Physiology, Medical University of Bialystok, 2c Mickiewicza Str., 15-222 Bialystok, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Górski
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000328:Adult; D001692:Biological Transport; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002518:Ceramides / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D000074026:Fatty Acid Binding Protein 3; D050612:Fatty Acid Transport Proteins / Q000235:genetics / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D006019:Glycolysis / Q000235:genetics*; D006801:Humans; D007044:Hysterectomy; D007889:Leiomyoma / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D010085:Oxidative Phosphorylation; D014280:Triglycerides / Q000378:metabolism
chemical_list: D015820:Cadherins; D002518:Ceramides; D004075:Diglycerides; C493408:FABP3 protein, human; C502403:FAT1 protein, human; D000074026:Fatty Acid Binding Protein 3; D050612:Fatty Acid Transport Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; C493568:SLC27A1 protein, human; C493571:SLC27A4 protein, human; D014280:Triglycerides; D015094:3-Hydroxyacyl CoA Dehydrogenases; D003576:Electron Transport Complex IV; D005947:Glucose
keywords: FAT/CD36; FATP-1; FATP-4; Fibroids; GLUT-1; GLUT-4