title: Bioaugmentation with GFP-Tagged Pseudomonas migulae AN-1 in Aniline-Contaminated Aquifer Microcosms: Cellular Responses, Survival and Effect on Indigenous Bacterial Community.
abstract: The recently isolated aniline-degrading bacterium Pseudomonas migulae AN-1 was tagged with green fluorescent protein (GFP) to investigate its bioaugmentation potential against anilinecontaminated groundwater through microcosm experiments. The survival and cellular response of GFP-tagged AN-1 introduced in a lab-scale aquifer corresponded directly with aniline consumption. During the process, the GFP-tagged AN-1 biomass increased from 7.52 × 10⁵ cells/ml to 128 × 10⁵ cells/ml and the degradation rate of aniline was 6.04 mg/l/h. GFP-tagged AN-1 was moderately hydrophobic (41.74%-47.69%) when treated with 20- 100 mg/l aniline and exhibited relatively strong hydrophobicity (55.25%-65.78%) when the concentration of aniline was ≥100 mg/l. The membrane permeability of AN-1 increased followed by a rise in aniline below 100 mg/l and was invariable with aniline above 100 mg/l. Pyrosequencing analysis showed that the relative abundance of Proteobacteria (accounted for 99.22% in the non-bioaugmentation samples) changed to 89.23% after bioaugmentation with GFP-tagged AN-1. Actinobacteria increased from 0.29% to 2.01%, whereas the abundance of Firmicutes barely changed. These combined findings demonstrate the feasibility of removing aniline in aquifers by introducing the strain AN-1 and provide valuable information on the changes in the diversity of dominant populations during bioaugmentation.
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, Changchun 130021, P.R. China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, Changchun 130021, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Qu
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, Changchun 130021, P.R. China.|Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA94720, USA.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, Changchun 130021, P.R. China.
authors.forename: Yunge
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, Changchun 130021, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, Changchun 130021, P.R. China.
authors.forename: Hejun
authors.initials: H
authors.lastname: Ren
mesh_terms: D039903:Actinobacteria / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism*; D058491:Bacterial Load; D001673:Biodegradation, Environmental; D018533:Biomass; D004269:DNA, Bacterial / Q000032:analysis; D052918:Environmental Restoration and Remediation; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D060587:Groundwater / Q000382:microbiology*; D020560:Proteobacteria / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014873:Water Pollutants / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D049452:Green Fluorescent Proteins; C023650:aniline
keywords: Aniline; Bioaugmentation; Cellular responses; GFP-tagged Pseudomonas migulae AN-1; Survival