title: Key KdSOC1 gene expression profiles during plantlet morphogenesis under hormone, photoperiod, and drought treatments.
abstract: Kalanchoe daigremontiana utilizes plantlet formation between its zigzag leaf margins as its method of asexual reproduction. In this study, K. daigremontiana SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (KdSOC1), a key intermediate in the transition from vegetative to asexual growth, was cloned. Furthermore, its expression profiles during plantlet formation under different environmental and hormone induction conditions were analyzed. The full-KdSOC1 cDNA sequence length was 1410 bp with 70% shared homology with Carya cathayensis SOC1. The conserved domain search of KdSOC1 showed the absence of I and C domains, which might indicate novel biological functions in K. daigremontiana. The full-KdSOC1 promoter sequence was 1401 bp long and contained multiple-hormone-responsive cis-acting elements. Hormone induction assays showed that gibberellins and salicylic acid mainly regulated KdSOC1 expression. The swift change from low to high KdSOC1 expression levels during long-day induction was accompanied by the rapid emergence of plantlets. Drought stress stimulated KdSOC1 expression in leaves both with and without plantlet formation. Together, the results suggested that KdSOC1 was closely involved in environmental stimulation signal perception and the transduction of K. daigremontiana plantlet formation. Therefore, future identification of KdSOC1 functions might reveal key information that will help elucidate the transition network between embryogenesis and organogenesis during plantlet formation.
authors.affiliation: Turfgrass Management Department, College of Forestry, Beijing Forestry University, Beijing, China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Biochemistry and Molecular Biology Department, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Turfgrass Management Department, College of Forestry, Beijing Forestry University, Beijing, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Zeng
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D031962:Kalanchoe / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008027:Light; D026161:MADS Domain Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017440:Photoperiod; D010802:Phylogeny; D063245:Plant Development / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012100:Reproduction, Asexual / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D026161:MADS Domain Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins