title: Intraperitoneal (188)Re-Liposome delivery switches ovarian cancer metabolism from glycolysis to oxidative phosphorylation and effectively controls ovarian tumour growth in mice.
abstract: Cancer stem cells exhibit distinctive cellular metabolism compared with the more differentiated counterparts or normal cells. We aimed to investigate the impact of a novel radionuclide anti-cancer agent (188)Re-Liposome on stemness markers' expression and cellular metabolism in an ovarian cancer model.
  
  
  
  A 2×2 factorial experiment was designed in which factor 1 represented the drug treatment comparing (188)Re-BMEDA, a free form of (188)Re, with (188)Re-Liposome, a nanoparticle-encapsulated form of (188)Re. Factor 2 represented the delivery route, comparing intravenous with intraperitoneal delivery.
  
  
  
  Intraperitoneal delivery of (188)Re-Liposome predominantly killed the CSCs-like cells in tumours and switched metabolism from glycolysis to oxidative phosphorylation. Further, intraperitoneal delivery of (188)Re-Liposome treatment was able to block epithelial-to-mesenchymal transition (EMT) and reactivate p53 function. Collectively, these molecular changes led to a striking tumour-killing effect.
  
  
  
  Radionuclides encapsulated in liposomes may represent a novel treatment for ovarian cancer when delivered intraperitoneally (a type of loco-regional delivery). In the future, this concept may be further extended for the treatment of several relevant cancers that have been proved to be suitable for loco-regional delivery of therapeutic agents, such as colon cancer, gastric cancer, and pancreatic cancer.
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Life Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yao An
authors.initials: YA
authors.lastname: Shen
authors.affiliation: Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Oncology, Taipei Veterans General Hospital, Taiwan.
authors.forename: Keng Li
authors.initials: KL
authors.lastname: Lan
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Life Sciences, National Yang-Ming University, Taipei, Taiwan; Institute of Nuclear Energy Research, Taiwan.
authors.forename: Chih Hsien
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Liang Ting
authors.initials: LT
authors.lastname: Lin
authors.affiliation: Department of Obstetrics and Gynaecology, Taipei Veterans General Hospital, Taiwan.
authors.forename: Chun Lin
authors.initials: CL
authors.lastname: He
authors.affiliation: Department of Obstetrics and Gynaecology, Taipei Veterans General Hospital, Taiwan.
authors.forename: Po Hung
authors.initials: PH
authors.lastname: Chen
authors.affiliation: Institute of Nuclear Energy Research, Taiwan.
authors.forename: Te Wei
authors.initials: TW
authors.lastname: Lee
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yi Jang
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Obstetrics and Gynaecology, Taipei Veterans General Hospital, Taiwan. Electronic address: cmjuang@gmail.com.
authors.forename: Chi Mu
authors.initials: CM
authors.lastname: Chuang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006019:Glycolysis; D006801:Humans; D008081:Liposomes; D051379:Mice; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D010085:Oxidative Phosphorylation; D011836:Radiation Tolerance; D011868:Radioisotopes / Q000008:administration & dosage*; D012211:Rhenium / Q000008:administration & dosage*; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
chemical_list: D008081:Liposomes; D011868:Radioisotopes; D016159:Tumor Suppressor Protein p53; D012211:Rhenium
keywords: (188)Re; Cancer stem cell; Liposome; Metabolic reprogramming; p53