title: [The expression of IDH1 (R132H) is positively correlated with cell proliferation and angiogenesis in glioma samples].
abstract: OBJECTIVE
  To explore the correlations of the expression of mutant isocitrate dehydrogenase (IDH1) (R132H) protein with angiogenesis and cell proliferation in glioma.
  
  
  METHODS
  We performed polymerase chain reaction-based IDH gene mutation screening in 385 glioma samples, and the subcellular localization and expression levels of IDH1 (R132H) was examined by immunohistochemistry (IHC). Ki-67 labeling index was introduced to determine the proliferation of glioma cells, and the microvessel density was measured through CD34 staining. Statistical analyses were performed to show the correlations of IDH1 mutation with cell proliferation and microvessel density.
  
  
  RESULTS
  The mutant rates of IDH1 were about 50%-60% in grade II-III gliomas and secondary glioblastomas, which were significantly higher than those in pilocytic astrocytoma (grade I) and primary glioblastoma (grade IV). Moreover, the level of IDH1 (R132H) protein was positively correlated with Ki-67 labeling index and microvessel density.
  
  
  CONCLUSIONS
  IDH mutation was common in grade II-III glioma and secondary glioblastoma, and the mutant IDH1 (R132H) might play a critical role in the cell proliferation and angiogenesis of glioma.
authors.affiliation: Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032; Department of Neurology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Jiankuan
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China. *Corresponding author, E-mail: yejing@fmmu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D018952:Antigens, CD34 / Q000378:metabolism; D001483:Base Sequence; D049109:Cell Proliferation / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005260:Female; D005910:Glioma / Q000098:blood supply / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007521:Isocitrate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D019394:Ki-67 Antigen; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human