title: Oleanolic Acid Inhibiting the Differentiation of Neural Stem Cells into Astrocyte by Down-Regulating JAK/STAT Signaling Pathway.
abstract: To investigate the effect of oleanolic acid (OA) on the differentiation of neural stem cells (NSCs) induced by A[Formula: see text] via regulating the JAK/STAT signaling pathway, a neurotoxicity cell model involving the induction of NSCs by soluble A[Formula: see text] (5 [Formula: see text]M) was used. The WST-1 method and immunofluorescence tests were used respectively to detect the activity of cell model and the expression of GFAP[Formula: see text]/DAPI and Tubulin[Formula: see text]/DAPI. Western blotting and real-time PCR analyses were used to observe the effects of OA on NSCs differentiation by examining key targets of the JAK/STAT signal transduction pathway. Compared with normal NSCs, A[Formula: see text]-induced NSCs had down-regulated expression of Ngn1 and up-regulated STAT3 expression and phosphorylation, and inhibited neuronal differentiation. OA treatment effectively inhibited the A[Formula: see text]-induced activation of JAK/STAT signaling, with a significant increase in Ngn1 expression and a significant decrease in p-STAT3/STAT3. These results indicate that OA could inhibit the excessive differentiation of NSCs into astrocytes by down-regulating JAK/STAT signaling which might retard the progress of AD.
authors.affiliation: * Department of Neurology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Yu-Lian
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: * Department of Neurology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: * Department of Neurology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Han
authors.affiliation: * Department of Neurology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: * Department of Neurology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Wan-Shan
authors.initials: WS
authors.lastname: Song
authors.affiliation: † Institute of Traditional Chinese and Western Medicine, Huashan Hospital Fudan University, Shanghai 200040, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Huang
authors.affiliation: * Department of Neurology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics*; D002478:Cells, Cultured; D003864:Depression, Chemical; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D053612:Janus Kinases; D008815:Mice, Inbred Strains; D058953:Neural Stem Cells / Q000166:cytology*; D009828:Oleanolic Acid / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D011247:Pregnancy; D050796:STAT3 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D050796:STAT3 Transcription Factor; C074636:amyloid beta-protein (25-35); D009828:Oleanolic Acid; D053612:Janus Kinases
keywords: A; JAK/STAT Signaling Pathway; NSCs Differentiation; Neural Stem Cells; Oleanolic Acid