title: Enhanced Boron Tolerance in Plants Mediated by Bidirectional Transport Through Plasma Membrane Intrinsic Proteins.
abstract: High boron (B) concentration is toxic to plants that limit plant productivity. Recent studies have shown the involvement of the members of major intrinsic protein (MIP) family in controlling B transport. Here, we have provided experimental evidences showing the bidirectional transport activity of rice OsPIP1;3 and OsPIP2;6. Boron transport ability of OsPIP1;3 and OsPIP2;6 were displayed in yeast HD9 mutant strain (∆fps1∆acr3∆ycf1) as a result of increased B sensitivity, influx and accumulation by OsPIP1;3, and rapid efflux activity by OsPIP2;6. RT-PCR analysis showed strong upregulation of OsPIP1;3 and OsPIP2;6 transcripts in roots by B toxicity. Transgenic Arabidopsis lines overexpressing OsPIP1;3 and OsPIP2;6 exhibited enhanced tolerance to B toxicity. Furthermore, B concentration was significantly increased after 2 and 3 hours of tracer boron ((10)B) treatment. Interestingly, a rapid efflux of (10)B from the roots of the transgenic plants was observed within 1 h of (10)B treatment. Boron tolerance in OsPIP1;3 and OsPIP2;6 lines was inhibited by aquaporin inhibitors, silver nitrate and sodium azide. Our data proved that OsPIP1;3 and OsPIP2;6 are indeed involved in both influx and efflux of boron transport. Manipulation of these PIPs could be highly useful in improving B tolerance in crops grown in high B containing soils.
authors.affiliation: Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.|Department of Biotechnology, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.|Department of Applied Biology, College of Sciences, University of Sharjah, P.O. Box 27272, Sharjah, United Arab Emirates.
authors.forename: Kareem A
authors.initials: KA
authors.lastname: Mosa
authors.affiliation: Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.|Department of Biological Sciences, Birla Institute of Technology &Science, K. K. Birla Goa Campus, Goa 403726, India.
authors.forename: Kundan
authors.initials: K
authors.lastname: Kumar
authors.affiliation: Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: Sudesh
authors.initials: S
authors.lastname: Chhikara
authors.affiliation: Department of Analytical Chemistry, The Connecticut Agricultural Experiment Station, New Haven, CT 06504-1106, USA.
authors.forename: Craig
authors.initials: C
authors.lastname: Musante
authors.affiliation: Department of Analytical Chemistry, The Connecticut Agricultural Experiment Station, New Haven, CT 06504-1106, USA.
authors.forename: Jason C
authors.initials: JC
authors.lastname: White
authors.affiliation: Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: Om Parkash
authors.initials: OP
authors.lastname: Dhankher
mesh_terms: D000222:Adaptation, Physiological; D020346:Aquaporins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D001895:Boron / Q000378:metabolism* / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D012835:Silver Nitrate / Q000494:pharmacology; D019810:Sodium Azide / Q000494:pharmacology
chemical_list: D020346:Aquaporins; D010940:Plant Proteins; D012835:Silver Nitrate; D019810:Sodium Azide; D001895:Boron