title: Enhanced SOFI algorithm achieved with modified optical fluctuating signal extraction.
abstract: In this paper, we present a modified SOFI algorithm with enhanced temporal resolution: the required number of raw images for SOFI is reduced from hundreds to tens. The modification is intended to eliminate the low-frequency fluctuation and readout noise from the raw image stack, and is achieved by separately utilizing two wavelet-based filters in the temporal and spatial domains of the raw image stack. The high-frequency stochastic fluctuating signal could be extracted effectively, and the efficiency of SOFI could be enhanced. The modified SOFI image could be generated with 25 frames of raw images, and the corresponding acquisition time was 1.25 s.
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haomin
authors.initials: H
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.forename: Xin
authors.initials: X
authors.lastname: Miao
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang