title: Tunable Carbon-Dot-Based Dual-Emission Fluorescent Nanohybrids for Ratiometric Optical Thermometry in Living Cells.
abstract: The use of carbon-dot-based dual-emission fluorescent nanohybrids (DEFNs) as versatile nanothermometry devices for spatially resolved temperature measurements in living cells is demonstrated. The carbon dots (CDs) are prepared in the organic phase and display tunable photoluminescence (PL) across a wide visible range by adjusting the excitation wavelengths and extend of N-doping. DEFNs are formed in a straightforward fashion from CDs (emitting blue PL) and gold nanoclusters (AuNCs, emitting red PL). The DEFNs display ideal single-excitation, dual-emission with two well-resolved, intensity-comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Furthermore, the DEFNs have been introduced into cells, exhibiting good biocompatibility, and have facilitated physiological temperature measurements in the range of 25-45 °C; the DEFNs can therefore function as "non-contact" tools for the accurate measurement of temperature and its gradient inside a living cell.
authors.affiliation: China-Australia Joint Research Centre for Functional Molecular Materials, School of Chemical & Material Engineering, Jiangnan University , Wuxi 214122, People's Republic of China.
authors.forename: Chuanxi
authors.initials: C
authors.lastname: Wang
authors.affiliation: China-Australia Joint Research Centre for Functional Molecular Materials, School of Chemical & Material Engineering, Jiangnan University , Wuxi 214122, People's Republic of China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Lin
authors.affiliation: China-Australia Joint Research Centre for Functional Molecular Materials, School of Chemical & Material Engineering, Jiangnan University , Wuxi 214122, People's Republic of China.
authors.forename: Zhenzhu
authors.initials: Z
authors.lastname: Xu
authors.affiliation: China-Australia Joint Research Centre for Functional Molecular Materials, School of Chemical & Material Engineering, Jiangnan University , Wuxi 214122, People's Republic of China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Research School of Chemistry, Australian National University , Canberra, ACT 2601, Australia.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Humphrey
authors.affiliation: China-Australia Joint Research Centre for Functional Molecular Materials, School of Chemical & Material Engineering, Jiangnan University , Wuxi 214122, People's Republic of China.|Research School of Chemistry, Australian National University , Canberra, ACT 2601, Australia.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002460:Cell Line; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D062125:Thermometry / Q000379:methods*
chemical_list: D002244:Carbon; D006046:Gold
keywords: carbon dots; dual-emission nanohybrid; living cells; nanothermometry; tunable fluorescence