title: Structural Proteomics of Herpesviruses.
abstract: Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have been unsuccessful because these viruses have developed numerous immune evasion mechanisms. Therefore, a better understanding of their complex lifecycle is needed. In particular, while the genome of numerous herpesviruses has been sequenced, the exact composition of virions remains unknown for most of them. Mass spectrometry has recently emerged as a central method and has permitted fundamental discoveries in virology. Here, we review mass spectrometry-based approaches that have recently allowed a better understanding of the composition of the herpesvirus virion. In particular, we describe strategies commonly used for proper sample preparation and fractionation to allow protein localization inside the particle but also to avoid contamination by nonstructural proteins. A collection of other important data regarding post-translational modifications or the relative abundance of structural proteins is also described. This review also discusses the poorly studied importance of host proteins in herpesvirus structural proteins and the necessity to develop a quantitative workflow to better understand the dynamics of the structural proteome. In the future, we hope that this collaborative effort will assist in the development of new strategies to fight these infections.
authors.affiliation: Laboratory of Proteomic and Microbiology, Research Institute of Biosciences, University of MONS, 4000 Mons, Belgium. baptiste.leroy@umons.ac.be.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Leroy
authors.affiliation: Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals and Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium. l.gillet@ulg.ac.be.
authors.forename: Laurent
authors.initials: L
authors.lastname: Gillet
authors.affiliation: Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals and Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium. a.vdplasschen@ulg.ac.be.
authors.forename: Alain
authors.initials: A
authors.lastname: Vanderplasschen
authors.affiliation: Laboratory of Proteomic and Microbiology, Research Institute of Biosciences, University of MONS, 4000 Mons, Belgium. ruddy.wattiez@umons.ac.be.
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
mesh_terms: D000818:Animals; D006564:Herpesviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006566:Herpesviridae Infections / Q000821:virology; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: herpesvirus; host proteins; proteomic; structural proteins