title: Effects of central irisin administration on the uncoupling proteins in rat brain.
abstract: Irisin is a thermogenic peptide that enables the development of brown adipose tissue from white adipose tissue by activating the UCP1. This study has been designed to determine the effects of the irisin on UCPs. Sprague Dawley female rats were used in the study. 1, 3 and 10μM concentrations of irisin were injected intracerebroventricularly to the rats, and the control group was received only vehicle. The animals were killed at the 16, 24, and 48h time intervals and their brains were taken out. The hypothalamus, pituitary gland, hippocampus, cerebellum, striatum and cortex areas were separated and the UCP2, UCP3, UCP4 and UCP5 mRNA levels were determined. Just before the animals were killed, their body temperatures were recorded. It was observed that after application of the high dose irisin, UCP5 mRNA level in the all brain areas increased (p<0.05); it was also observed that the three doses decreased the UCP4 expression in all brain areas (except the pituitary gland; p<0.05). The UCP2 and UCP3 mRNA expressions showed significantly increase in cerebellum and striatum (p<0.05). The UCP2 mRNA expression decreased in hypothalamus, pituitary gland, hippocampus and cortex areas (p<0.05). It was also observed that the body temperatures of the rats increased depending on the irisin injection and this increase was the most considerable at the 24h (p<0.05). The results of this study suggest that the UCP2-5 is expressed in different areas of the brain, and the irisin affects this expression, and may have effective roles in some brain functions.
authors.affiliation: Department of Molecular Biology and Genetic, Faculty of Science, Bartin University, Bartin, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Erden
authors.affiliation: Department of Physiology, Faculty of Medicine, Inonu University, Malatya 44280, Turkey.
authors.forename: Suat
authors.initials: S
authors.lastname: Tekin
authors.affiliation: Department of Physiology, Faculty of Medicine, Inonu University, Malatya 44280, Turkey. Electronic address: suleyman.sandal@inonu.edu.tr.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Sandal
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Firat University, Elazig, Turkey.
authors.forename: Ebru Etem
authors.initials: EE
authors.lastname: Onalan
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Firat University, Elazig, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Tektemur
authors.affiliation: Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.
authors.forename: Sevda
authors.initials: S
authors.lastname: Kirbag
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D001921:Brain / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000378:metabolism* / Q000494:pharmacology; D007276:Injections, Intraventricular; D007473:Ion Channels / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D000071245:Mitochondrial Uncoupling Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
chemical_list: C583676:FNDC5 protein, rat; D005353:Fibronectins; D007473:Ion Channels; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D009419:Nerve Tissue Proteins; C492125:Slc25a14 protein, rat; C494853:Slc25a27 protein, rat; C000604391:Ucp2 protein, rat; C000604394:Ucp3 protein, rat; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
keywords: Body temperature; Irisin; Rat brain; UCP2; UCP3; UCP4; UCP5