title: Anti-Bacterial effect of Aqueous Garlic Extract (AGE) determined by Disc Diffusion Method against Escherichia coli.
abstract: The study was performed to determine the antibacterial effect of aqueous extract of garlic (Allium sativum) against standard strain of Escherichia coli ATCC 25922. An interventional study was conducted in Department of Pharmacology and Therapeutics in collaboration with Department of Microbiology, Mymensingh Medical College, Mymensingh. Antibacterial effect of AGE was determined by disc diffusion method. Sensitivity of AGE determined in disc diffusion and the zone of inhibition (ZOI) was 4 mm, 10 mm and 20 mm at 25 μg/10 μl, 50 μg/10 μl and 100 μg/10 μl concentrations respectively. From the findings it is clearly determined the extract has definite antibacterial effect upon Escherichia coli. Further studies are required to detect and isolate the active ingredients present in the Garlic extract as well as detail steps of mechanism responsible for antibacterial effect. Then their effects against the studied organism should be studied in vivo separately and its toxicity profile should also be taken into account.
authors.affiliation: Dr Sanjoy Saha, M. Phil (Thesis Part), Department of Pharmacology & Therapeutics, Mymensingh Medical College (MMC), Mymensingh, Bangladesh.
authors.forename: S
authors.initials: S
authors.lastname: Saha
authors.forename: S K
authors.initials: SK
authors.lastname: Saha
authors.forename: M A
authors.initials: MA
authors.lastname: Hossain
authors.forename: S K
authors.initials: SK
authors.lastname: Paul
authors.forename: R R
authors.initials: RR
authors.lastname: Gomes
authors.forename: M
authors.initials: M
authors.lastname: Imtiaz
authors.forename: M M
authors.initials: MM
authors.lastname: Islam
authors.forename: H
authors.initials: H
authors.lastname: Nahar
authors.forename: S A
authors.initials: SA
authors.lastname: Begum
authors.forename: T T
authors.initials: TT
authors.lastname: Mirza
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D005737:Garlic / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts