title: Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers.
abstract: The application of nanotechnology is important to improve research and development of alternative anticancer therapies. In order to accelerate research related to cancer diagnosis and to improve the effectiveness of cancer treatment, various nanomaterials are being tested. The main objective of this work was basic research focused on examination of the mechanism and effectiveness of the introduction of nanoencapsulated photosensitizers to human carcinoma (A549) and normal cells (MRC-5). Newly encapsulated hydrophobic indocyanine-type photosensitizer (i.e., IR-780) was subjected to in vitro studies to determine its release characteristics on a molecular level. The photosensitizers were delivered to carcinoma and normal cells cultured under model conditions using multiwell plates and with the use of the specially designed hybrid (poly(dimethylsiloxane) (PDMS)/glass) microfluidic system. The specific geometry of our microsystem allows for the examination of intercellular interactions between cells cultured in the microchambers connected with microchannels of precisely defined length. Our microsystem allows investigating various therapeutic procedures (e.g., photodynamic therapy) on monoculture, coculture, and mixed culture, simultaneously, which is very difficult to perform using standard multiwell plates. In addition, we tested the cellular internalization of nanoparticles (differing in size, surface properties) in carcinoma and normal lung cells. We proved that cellular uptake of nanocapsules loaded with cyanine IR-780 in carcinoma cells was more significant than in normal cells. We demonstrated non cytotoxic effect of newly synthesized nanocapsules built with polyelectrolytes (PEs) of opposite surface charges: polyanion-polysodium-4-styrenesulphonate and polycation-poly(diallyldimethyl-ammonium) chloride loaded with cyanine IR-780 on human lung carcinoma and normal cell lines. However, the differences observed in the photocytotoxic effect between two types of tested nanocapsules can result from the type of last PE layer and their different surface charge.
authors.affiliation: Institute of Biotechnology, Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Jastrzębska
authors.affiliation: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Technology , Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Bazylińska
authors.affiliation: Institute of Biotechnology, Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bułka
authors.affiliation: Institute of Biotechnology, Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Tokarska
authors.affiliation: Institute of Biotechnology, Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Chudy
authors.affiliation: Institute of Biotechnology, Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Dybko
authors.affiliation: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Technology , Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
authors.forename: Kazimiera Anna
authors.initials: KA
authors.lastname: Wilk
authors.affiliation: Institute of Biotechnology, Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology , Noakowskiego 3, 00-664 Warsaw, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Brzózka