title: Fluoroquinolone and macrolide resistance in Campylobacter jejuni isolated from broiler slaughterhouses in southern Brazil.
abstract: Campylobacter jejuni is recognized as a leading cause of acute bacterial gastroenteritis in humans. The over-use of antimicrobials in the human population and in animal husbandry has led to an increase in antimicrobial-resistant infections, particularly with fluoroquinolones and macrolides. The aim of the present study was to provide information of the current status of antimicrobial resistance patterns in Campylobacter jejuni from poultry sources. Fifty strains were recovered from broiler slaughterhouses in Rio Grande do Sul state, Brazil, 2012. The strains were investigated for antimicrobial susceptibility against three agents (ciprofloxacin, nalidixic acid and erythromycin) by minimal inhibitory concentrations. The strains were analysed by polymerase chain reaction-restriction fragment length polymorphism for detection of the Thr-86 mutation that confers resistance to ciprofloxacin. In addition, all the strains were tested for the presence of efflux systems (cmeB gene) conferring antimicrobial resistance. The minimum inhibitory concentrations results showed that 98% of isolates were sensitive to erythromycin and most isolates were resistant to ciprofloxacin (94%) and nalidixic acid (90%). A complete correlation was observed between the minimum inhibitory concentrations and PCR-RFLP assay. Finally, the cmeB gene that is responsible for multidrug resistance was detected in 16 isolates out the 50 strains (32%).
authors.affiliation: a Poultry Diagnostic and Research Centre (CDPA), Faculty of Veterinary Medicine , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
authors.forename: Yuli M
authors.initials: YM
authors.lastname: Sierra-Arguello
authors.affiliation: a Poultry Diagnostic and Research Centre (CDPA), Faculty of Veterinary Medicine , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Perdoncini
authors.affiliation: a Poultry Diagnostic and Research Centre (CDPA), Faculty of Veterinary Medicine , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
authors.forename: R B
authors.initials: RB
authors.lastname: Morgan
authors.affiliation: a Poultry Diagnostic and Research Centre (CDPA), Faculty of Veterinary Medicine , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
authors.forename: C T P
authors.initials: CT
authors.lastname: Salle
authors.affiliation: a Poultry Diagnostic and Research Centre (CDPA), Faculty of Veterinary Medicine , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
authors.forename: H L S
authors.initials: HL
authors.lastname: Moraes
authors.affiliation: b Laboratory of Veterinary Bacteriology (LABACVET) , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
authors.forename: Marcos J P
authors.initials: MJ
authors.lastname: Gomes
authors.affiliation: a Poultry Diagnostic and Research Centre (CDPA), Faculty of Veterinary Medicine , Federal University of Rio Grande do Sul (UFRGS) , Porto Alegre , Brazil.
authors.forename: Vladimir Pinheiro
authors.initials: VP
authors.lastname: do Nascimento
mesh_terms: D000003:Abattoirs; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil / Q000453:epidemiology; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology*; D002939:Ciprofloxacin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D004917:Erythromycin / Q000494:pharmacology; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009268:Nalidixic Acid / Q000494:pharmacology; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D018942:Macrolides; D009268:Nalidixic Acid; D002939:Ciprofloxacin; D004917:Erythromycin
keywords: Campylobacter jejuni; MIC; PCR; RFLP; fluoroquinolones; macrolides