title: Intranasal treatment with bacteriophage rescues mice from Acinetobacter baumannii-mediated pneumonia.
abstract: With the emergence of drug-resistant bacteria, finding alternative agents to treat antibiotic-resistant bacterial infections is imperative.
  
  
  
  A mouse pneumonia model was developed by combining cyclophosphamide pretreatment and Acinetobacter baumannii challenge, and a lytic bacteriophage was evaluated for its therapeutic efficacy in this model by examining the survival rate, bacterial load in the lung and lung pathology.
  
  
  
  Intranasal instillation with bacteriophage rescued 100% of mice following lethal challenge with A. baumannii. Phage treatment reduced bacterial load in the lung. Microcomputed tomography indicated a reduction in lung inflammation in mice given phage.
  
  
  
  This research demonstrates that intranasal application of bacteriophage is viable, and could provide complete protection from pneumonia caused by A. baumannii.
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Mi
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: An
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Puyuan
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Xianglilan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Respiratory Medicine, the First Hospital of Changsha, 410000, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Peng
authors.affiliation: Department of Respiratory Medicine, PLA Hospital 307, Beijing 100071, China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Bai
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology / Q000628:therapy*; D040981:Acinetobacter baumannii / Q000502:physiology / Q000821:virology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D058491:Bacterial Load; D001435:Bacteriophages; D001691:Biological Therapy; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011014:Pneumonia / Q000188:drug therapy / Q000382:microbiology / Q000628:therapy*
chemical_list: D000900:Anti-Bacterial Agents; D003520:Cyclophosphamide
keywords: Acinetobacter baumannii; antibody; bacteriophage; cyclophosphamide; mouse model; pneumonia