title: Exosomes isolation and characterization in serum is feasible in non-small cell lung cancer patients: critical analysis of evidence and potential role in clinical practice.
abstract: Exosomes are nano-sized vesicles of endolysosomal origin, released by several cytotypes in physiological and pathological conditions. Tumor derived exosomes, interacting with other cells of the tumor microenvironment, modulate tumor progression, angiogenic switch, metastasis, and immune escape. Recently, extracellular vesicles were proposed as excellent biomarkers for disease monitoring and prognosis in cancer patients. Non-small cell lung cancer (NSCLC) has a poor 5-year survival rate due to the delay in the detection of the disease. The majority of patients are diagnosed in an advanced disease stage. Exosomes might be promising beneficial tools as biomarker candidates in the scenario of NSCLC, since they contain both, proteins and miRNAs. The clinical case reported in this manuscript is a proof of concept revealing that NSCLC exosomes and sorted miRNAs might constitute, in a near future, novel biomarkers. This review summarizes the role of exosomes in NSCLC, focusing on the importance of exosomal microRNAs in lung cancer diagnosis and prognosis.
authors.affiliation: Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, Palermo, Italy.|Institute of Biomedicine and Molecular Immunology (IBIM), National Research Council, Palermo, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Taverna
authors.affiliation: Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, Palermo, Italy.|Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA) and Center for Oncological Research (CORE) Antwerp University, Wilrijkstraat, Edegem, Antwerp, Belgium.
authors.forename: Marco
authors.initials: M
authors.lastname: Giallombardo
authors.affiliation: Department of Oncology, Clínica Universidad de Navarra, Pamplona, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Gil-Bazo
authors.affiliation: Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA) and Center for Oncological Research (CORE) Antwerp University, Wilrijkstraat, Edegem, Antwerp, Belgium.
authors.forename: Anna Paola
authors.initials: AP
authors.lastname: Carreca
authors.affiliation: Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA) and Center for Oncological Research (CORE) Antwerp University, Wilrijkstraat, Edegem, Antwerp, Belgium.
authors.forename: Marta
authors.initials: M
authors.lastname: Castiglia
authors.affiliation: Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA) and Center for Oncological Research (CORE) Antwerp University, Wilrijkstraat, Edegem, Antwerp, Belgium.
authors.forename: Jorge
authors.initials: J
authors.lastname: Chacártegui
authors.affiliation: Service of Medical Oncology, Centro Hospitalar do Porto, Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal.
authors.forename: Antonio
authors.initials: A
authors.lastname: Araujo
authors.affiliation: Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, Palermo, Italy.|Institute of Biomedicine and Molecular Immunology (IBIM), National Research Council, Palermo, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Alessandro
authors.affiliation: Molecular Pathology, Pathology Department, Antwerp University Hospital (UZA) and Center for Oncological Research (CORE) Antwerp University, Wilrijkstraat, Edegem, Antwerp, Belgium.
authors.forename: Patrick
authors.initials: P
authors.lastname: Pauwels
authors.affiliation: Oncology Department, Antwerp University Hospital (UZA) and Center for Oncological Research (CORE) Antwerp University, Wilrijkstraat, Edegem, Antwerp, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Peeters
authors.affiliation: Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA) and Center for Oncological Research (CORE) Antwerp University, Wilrijkstraat, Edegem, Antwerp, Belgium.
authors.forename: Christian
authors.initials: C
authors.lastname: Rolfo
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics / Q000276:immunology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics / Q000276:immunology*; D018450:Disease Progression; D055354:Exosomes / Q000235:genetics / Q000276:immunology*; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics / Q000276:immunology*; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000276:immunology; D011379:Prognosis; D059016:Tumor Microenvironment / Q000235:genetics / Q000276:immunology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: NSCLC; biomarkers; exosomes; liquid biopsies; microRNAs