title: The Effect of Epigallocatechin Gallate on Flap Viability of Rat Perforator Abdominal Flaps.
abstract: Background Epigallocatechin gallate (EGCG) is a substance abundant in green tea. In this study, the effects of EGCG on perforator flap viability were investigated. Methods A total of 40 rats were assigned to four groups of 10 each. In each subject, a 4 × 6 cm abdominal skin flap was raised and adapted back onto its place. In the control group, no further procedures were taken. In the flap group, 40 mg/kg/d EGCG was injected into the flap. In the gavage group, 100 mg/kg/d EGCG was given through a feeding tube. In the intraperitoneal group, 50 mg/kg/d EGCG was injected intraperitoneally. On the 7th postoperative day, flaps were photographed and the viable areas were measured and compared via a one-way analysis of variance. Results The ratios of viable and contracted flap area were 9.15/12.01, 4.59/16.46, 11.56/11.20, and 11.65/10.77 cm(2) for the control, flap group, gavage group, and intraperitoneal group, respectively. While the flap group yielded the worst results in the sense of flap contraction and viability (p < 0.001), the gavage and intraperitoneal groups were significantly better than those of the control group (p = 0.03). Histologically, epidermal, papillary dermal, and capillary tissue volumes were evaluated. In comparison to the control group, the flap group yielded significantly increased epidermal and dermal volumes (p = 0.03), however, these values were significantly decreased (p = 0.04) in the gavage and intraperitoneal groups. Capillary volumes were significantly decreased in EGCG treatment groups (p < 0.01). Conclusion Our experiment has shown that oral and intraperitoneal administration of EGCG increases the perforator flap viability when compared with controls, while direct injection decreases the viability.
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, Samsun Education and Research Hospital, Samsun, Turkey.
authors.forename: İbrahim Alper
authors.initials: İA
authors.lastname: Aksakal
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Küçüker
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Mehmet Emin
authors.initials: ME
authors.lastname: Önger
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Murat Sinan
authors.initials: MS
authors.lastname: Engin
authors.affiliation: Deartment of Plastic, Reconstructive and Aesthetic Surgery, Diskapi Yildirim Beyazit Training and Research Hospital Ankara, Turkey.
authors.forename: Musa Kemal
authors.initials: MK
authors.lastname: Keleş
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Demir
mesh_terms: D000005:Abdomen / Q000473:pathology*; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D006085:Graft Survival / Q000187:drug effects*; D061525:Perforator Flap / Q000098:blood supply / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D019651:Plastic Surgery Procedures / Q000379:methods*; D012039:Regional Blood Flow
chemical_list: D043925:Angiogenesis Inducing Agents; D002392:Catechin; C045651:epigallocatechin gallate