title: Molecular cloning, expression, and stress response of the estrogen-related receptor gene (AccERR) from Apis cerana cerana.
abstract: Estrogen-related receptor (ERR), which belongs to the nuclear receptor superfamily, has been implicated in diverse physiological processes involving the estrogen signaling pathway. However, little information is available on ERR in Apis cerana cerana. In this report, we isolated the ERR gene and investigated its involvement in antioxidant defense. Quantitative real-time polymerase chain reaction (qPCR) revealed that the highest mRNA expression occurred in eggs during different developmental stages. The expression levels of AccERR were highest in the muscle, followed by the rectum. The predicted transcription factor binding sites in the promoter of AccERR suggested that AccERR potentially functions in early development and in environmental stress responses. The expression of AccERR was induced by cold (4 °C), heat (42 °C), ultraviolet light (UV), HgCl2, and various types of pesticides (phoxim, deltamethrin, triadimefon, and cyhalothrin). Western blot was used to measure the expression levels of AccERR protein. These data suggested that AccERR might play a vital role in abiotic stress responses.
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
authors.forename: Weixing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China.
authors.forename: Xingqi
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, People's Republic of China. bhxu@sdau.edu.cn.
authors.forename: Baohua
authors.initials: B
authors.lastname: Xu
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D011960:Receptors, Estrogen / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D011960:Receptors, Estrogen
keywords: Apis cerana cerana; Biochemical properties; Estrogen-related receptor; Oxidative stress; qPCR