title: [Significance of Morphological Examination, Cytochemical Staining Combined with Bone Marrow Biopsy in Differential Diagnosis of Myelodysplastic Syndrome with Low Blasts and Hemolytic Anemia].
abstract: OBJECTIVE
  To explore the value of morphological examination, cytochemical staining combined with bone marrow biopsy in the differential diagnosis between myelodysplastic syndrome (MDS) with low blasts and hemolytic anemia (HA).
  
  
  METHODS
  The clinical data of 85 cases of myelodysplastic syndrome with low blasts (< 5%) and 61 patients with hemolytic anemia in Chinese PLA's Gerneral hospital from September 2009 to March 2015 were retrospectively analysed. The clinical characteristics, cytogenetic and molecular features, bone marrow cell count and morphology features, cytochemical staining results and bone marrow biopsy features of above-methioned patients were compared.
  
  
  RESULTS
  There was no significant difference (P > 0.05) in clinical data between MDS group and HA group. Megakaryocytic dysplasia-positive rate, and ring sideroblasts positive rate, and PAS positive rate were significantly higher in MDS group than those that in HA group (P < 0.05). Abnormal localization of immature precursors (ALIP) and megakaryocytic dysplasia positive rate in bone marrow biopsy were significantly higher in MDS group than those that in HA group (P < 0.05), 90.6% of MDS with low blasts patients were identifiable by combined detections.
  
  
  CONCLUSIONS
  Combining detection of morphology, cytochemistry staining and bone marrow biopsy has been confirmed to be more useful for differential diagnosis between MDS with low blasts and HA.
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China.|Department of Laboratorial Examination, Chinese PLA 305 Hospital, Beijing 100017, China.
authors.forename: Li-Lin
authors.initials: LL
authors.lastname: Gu
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Kang
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Gai-Xia
authors.initials: GX
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Su-Jun
authors.initials: SJ
authors.lastname: Ge
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China. E-mail: limianyang301@163.com.
authors.forename: Mian-Yang
authors.initials: MY
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratorial Examination, Chinese PLA General Hospital, Beijing 100853, China. E-mail: wangcb301@126.com.
authors.forename: Cheng-Bin
authors.initials: CB
authors.lastname: Wang
mesh_terms: D000743:Anemia, Hemolytic / Q000150:complications / Q000175:diagnosis*; D001706:Biopsy; D001854:Bone Marrow Cells / Q000166:cytology; D003937:Diagnosis, Differential; D015672:Erythroid Precursor Cells / Q000166:cytology; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology; D009190:Myelodysplastic Syndromes / Q000150:complications / Q000175:diagnosis*; D012189:Retrospective Studies; D013194:Staining and Labeling