title: Engineering hybrid exosomes by membrane fusion with liposomes.
abstract: Exosomes are a valuable biomaterial for the development of novel nanocarriers as functionally advanced drug delivery systems. To control and modify the performance of exosomal nanocarriers, we developed hybrid exosomes by fusing their membranes with liposomes using the freeze-thaw method. Exosomes embedded with a specific membrane protein isolated from genetically modified cells were fused with various liposomes, confirming that membrane engineering methods can be combined with genetic modification techniques. Cellular uptake studies performed using the hybrid exosomes revealed that the interactions between the developed exosomes and cells could be modified by changing the lipid composition or the properties of the exogenous lipids. These results suggest that the membrane-engineering approach reported here offers a new strategy for developing rationally designed exosomes as hybrid nanocarriers for use in advanced drug delivery systems.
authors.affiliation: JST-ERATO, Akiyoshi Bio-nanotransporter Project, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Yuko T
authors.initials: YT
authors.lastname: Sato
authors.affiliation: JST-ERATO, Akiyoshi Bio-nanotransporter Project, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Umezaki
authors.affiliation: JST-ERATO, Akiyoshi Bio-nanotransporter Project, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.|Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sawada
authors.affiliation: JST-ERATO, Akiyoshi Bio-nanotransporter Project, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.|Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Sada-atsu
authors.initials: SA
authors.lastname: Mukai
authors.affiliation: Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sasaki
authors.affiliation: Department of Immuno-Gene Therapy, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan.
authors.forename: Naozumi
authors.initials: N
authors.lastname: Harada
authors.affiliation: Department of Immuno-Gene Therapy, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiku
authors.affiliation: JST-ERATO, Akiyoshi Bio-nanotransporter Project, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.|Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D055354:Exosomes / Q000737:chemistry / Q000378:metabolism*; D005434:Flow Cytometry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D005615:Freezing; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008561:Membrane Fusion; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D000067996:RAW 264.7 Cells; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D008081:Liposomes; D018719:Erb-b2 Receptor Tyrosine Kinases