title: Allogeneic Mesenchymal Stem Cells in Combination with Hyaluronic Acid for the Treatment of Osteoarthritis in Rabbits.
abstract: Mesenchymal stem cell (MSC)-based therapies may aid in the repair of articular cartilage defects. The purpose of this study was to investigate the effects of intraarticular injection of allogeneic MSCs in an in vivo anterior cruciate ligament transection (ACLT) model of osteoarthritis in rabbits. Allogeneic bone marrow-derived MSCs were isolated and cultured under hypoxia (1% O2). After 8 weeks following ACLT, MSCs suspended in hyaluronic acid (HA) were injected into the knees, and the contralateral knees were injected with HA alone. Additional controls consisted of a sham operation group as well as an untreated osteoarthritis group. The tissues were analyzed by macroscopic examination as well as histologic and immunohistochemical methods at 6 and 12 weeks post-transplantation. At 6 and 12 weeks, the joint surface showed less cartilage loss and surface abrasion after MSC injection as compared to the tissues receiving HA injection alone. Significantly better histological scores and cartilage content were observed with the MSC transplantation. Furthermore, engraftment of allogenic MSCs were evident in surface cartilage. Thus, injection of the allogeneic MSCs reduced the progression of osteoarthritis in vivo.
authors.affiliation: Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Department of Surgery, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: En-Rung
authors.initials: ER
authors.lastname: Chiang
authors.affiliation: Department of Surgery, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: Hsiao-Li
authors.initials: HL
authors.lastname: Ma
authors.affiliation: Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Department of Surgery, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: Jung-Pan
authors.initials: JP
authors.lastname: Wang
authors.affiliation: Department of Surgery, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: Chien-Lin
authors.initials: CL
authors.lastname: Liu
authors.affiliation: Department of Surgery, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan.
authors.forename: Tain-Hsiung
authors.initials: TH
authors.lastname: Chen
authors.affiliation: Institute of Clinical Medicine, National Yang-Ming University, Taipei 112, Taiwan.|Institute of Pharmacology, National Yang-Ming University, Taipei 112, Taiwan.|Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 112, Taiwan.|Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan.|Integrative Stem Cell Center & Department of Orthopedics, China Medical University Hospital, Taichung 404, Taiwan.|Institute of Clinical Medicine, China Medical University, Taichung 404, Taiwan.
authors.forename: Shih-Chieh
authors.initials: SC
authors.lastname: Hung
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D002454:Cell Differentiation / Q000187:drug effects; D015687:Cell Hypoxia / Q000187:drug effects; D024043:Collagen Type II / Q000378:metabolism; D024121:Collagen Type X / Q000378:metabolism; D005269:Femur / Q000187:drug effects / Q000473:pathology; D006820:Hyaluronic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D010003:Osteoarthritis / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects; D013977:Tibia / Q000187:drug effects / Q000473:pathology; D014184:Transplantation, Homologous
chemical_list: D024043:Collagen Type II; D024121:Collagen Type X; D006820:Hyaluronic Acid