title: Uncommon functional properties of the first piscine 26S proteasome from the Antarctic notothenioid Trematomus bernacchii.
abstract: Protein homoeostasis is a fundamental process allowing the preservation of functional proteins and it has a great impact on the life of the Antarctic organisms. However, the effect of low temperatures on protein turnover is poorly understood and the cold-adaptation of the degradation machinery remains an unresolved issue. As the 26S proteasome represents the main proteolytic system devoted to the controlled degradation of intracellular proteins, the purpose of the present study was to investigate the functions of this complex in the notothenioid Trematomus bernacchii, in order to better understand its role in the physiology of Antarctic fish. To this aim, we purified and characterized the 26S proteasome from T. bernacchii and isolated the cDNAs codifying seven of the 14 subunits belonging to the proteasome 20S core particle. Results provided evidences of the high resistance of the piscine 26S proteasome to oxidative agents and of its 'uncommon' ability to efficiently hydrolyse oxidized bovine serum albumin (BSA), suggesting that this enzymatic complex could play a key role in the antioxidant defense systems in fish inhabiting permanently cold marine environments. These unique properties were also reflected by the 3D model analysis, which revealed a higher structural stability of the piscine complex respect to the murine template. Finally, a comparative analysis, performed in a variety of tissues collected from T. bernacchii and the temperate fish Dicentrarchus labrax, showed a lower protein retention in the cold-adapted fish, possibly due to a better efficiency of its degradation machinery.
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Gogliettino
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Balestrieri
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Riccio
authors.affiliation: Institute of Food Sciences, National Research Council (CNR-ISA), Via Roma 64, 83100 Avellino, Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: Facchiano
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: Fusco
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Vincenzo Cecere
authors.initials: VC
authors.lastname: Palazzo
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Mosè
authors.initials: M
authors.lastname: Rossi
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy ennio.cocca@ibbr.cnr.it.
authors.forename: Ennio
authors.initials: E
authors.lastname: Cocca
authors.affiliation: Institute of Biosciences and BioResources, National Research Council (CNR-IBBR), Via Pietro Castellino 111, 80131 Napoli, Italy.
authors.forename: Gianna
authors.initials: G
authors.lastname: Palmieri
mesh_terms: D000818:Animals; D002417:Cattle; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010473:Perciformes / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease
keywords: 26S proteasome; Antarctic notothenioid Trematomus bernacchii; cold adaptation; oxidized protein degradation; protein degradation machinery