title: [Inductive Effect of 5-Azacitidine on Apoptosis of Multiple Myeloma Cell lines and Its Mechanism].
abstract: OBJECTIVE
  To investigate the response of multiple myeloma (MM) cells to 5-azacitidine and its possible mechanisms.
  
  
  METHODS
  Two multiple myeloma-derived cell lines U266 and H929 were used in this study. The cell proliferation and apoptosis were assessed by CCK-8, flow cytometry and acridine orange staining. The cell cycle was assessed by flow cytometry. The expression of BCL-2, BAX was assessed by RT-PCR. The expressions of caspase-3 and p-ERK1/2 were assessed by Western blot.
  
  
  RESULTS
  The BCL-2/BAX ratio decreased, the activity of caspase-3 and p-ERK1/2 increased, the cell cycle was arrested in G2/M phase after treatment with 5-azacitidine. The 5-azacitidine inhibited proliferation in a dose-dependent manner.
  
  
  CONCLUSIONS
  5-azacitidine exerts apoptosis-inducing and grow-inhibiting effects on MM cell lines, its mechanism may be related with the decrease of BCL-2/BAX ratio, caspase-3 activation and the arrest of cell cycle.
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Zhu
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China. E-mail: jinghm@yahoo.com.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Jing
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001374:Azacitidine / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009101:Multiple Myeloma / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C505407:CASP3 protein, human; D053148:Caspase 3; D001374:Azacitidine