title: [miR-101 inhibits the proliferation and migration of breast cancer cells via downregulating the expression of DNA methyltransferase 3a].
abstract: OBJECTIVE
  To investigate the effect of microRNA-101 (miR-101) on the proliferation and migration of breast cancer cells and its possible mechanism.
  
  
  METHODS
  The expressions of miR-101 and DNA methyltransferase 3a (DNMT3a) in breast cancer tissues, corresponding normal breast tissues, breast cancer cells and normal breast cells were detected by real-time quantitative PCR. The lentiviral vectors containing miR-101 and shRNA-DNMT3a sequences were transfected into MDA-MB-231 cells to regulate the expressions of miR-101 and DNMT3a. The expressions of DNMT3a and E-cadherin were determined by Western blotting. The proliferation and migration abilities of MDA-MB-231 cells were evaluated by MTT assay and wound healing assay, respectively.
  
  
  RESULTS
  Breast cancer tissues and breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-361, HCC70) showed lower miR-101 expression and higher DNMT3a expression than the adjacent normal breast tissues and normal breast cell line. The overexpression of miR-101 resulted in downregulation of DNMT3a and restoration of E-cadherin. Besides, knockdown of DNMT3a by shRNA increased E-cadherin expression. MTT assay and would healing assay showed that miR-101 overexpression significantly inhibited the proliferation and migration abilities of MDA-MB-231 cells.
  
  
  CONCLUSIONS
  miR-101 may inhibit MDA-MB-231 cell proliferation and migration by repressing DNMT3a expression and up-regulating E-cadherin expression.
authors.affiliation: Department of Thoracic Surgery and Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Respiration, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Pang
authors.affiliation: Department of Thoracic Surgery and Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Huangzhen
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery and Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Radiation Oncology, Fudan University, Shanghai Cancer Center, Shanghai 200032, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Thoracic Surgery and Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. *Corresponding authors, E-mail: kissable_liu@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Thoracic Surgery and Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. *Corresponding authors, E-mail: renh_med@163.com.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism; D000090205:DNA Methyltransferase 3A; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D008856:Microscopy, Fluorescence; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015820:Cadherins; C000717967:DNMT3A protein, human; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A