title: Bile Diversion in Roux-en-Y Gastric Bypass Modulates Sodium-Dependent Glucose Intestinal Uptake.
abstract: Gastro-intestinal exclusion by Roux-en-Y gastric bypass (RYGB) improves glucose metabolism, independent of weight loss. Although changes in intestinal bile trafficking have been shown to play a role, the underlying mechanisms are unclear. We performed RYGB in minipigs and showed that the intestinal uptake of ingested glucose is blunted in the bile-deprived alimentary limb (AL). Glucose uptake in the AL was restored by the addition of bile, and this effect was abolished when active glucose intestinal transport was blocked with phlorizin. Sodium-glucose cotransporter 1 remained expressed in the AL, while intraluminal sodium content was markedly decreased. Adding sodium to the AL had the same effect as bile on glucose uptake. It also increased postprandial blood glucose response in conscious minipigs following RYGB. The decrease in intestinal uptake of glucose after RYGB was confirmed in humans. Our results demonstrate that bile diversion affects postprandial glucose metabolism by modulating sodium-glucose intestinal cotransport.
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Gregory
authors.initials: G
authors.lastname: Baud
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Daoudi
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hubert
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Violeta
authors.initials: V
authors.lastname: Raverdy
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Pigeyre
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Erik
authors.initials: E
authors.lastname: Hervieux
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Devienne
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ghunaim
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Bonner
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Quenon
authors.affiliation: University Lille, Inserm, CHU Lille, U1172 Jean-Pierre Aubert Research Center, 59000 Lille, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Pigny
authors.affiliation: CHU Lille, Metabolism and Glycosylation Diseases, Biology Pathology Center, 59000 Lille, France.
authors.forename: André
authors.initials: A
authors.lastname: Klein
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Kerr-Conte
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Valery
authors.initials: V
authors.lastname: Gmyr
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Caiazzo
authors.affiliation: University Lille, Inserm, CHU Lille, U1190 Translational research for diabetes, 59000 Lille, France; European Genomic Institute for Diabetes, EGID, 59000 Lille, France. Electronic address: fpattou@univ-lille2.fr.
authors.forename: François
authors.initials: F
authors.lastname: Pattou
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000502:physiology*; D001692:Biological Transport; D005260:Female; D015390:Gastric Bypass; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000601:surgery; D012964:Sodium / Q000378:metabolism; D051247:Sodium-Glucose Transport Proteins / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature
chemical_list: D001647:Bile Acids and Salts; D051247:Sodium-Glucose Transport Proteins; D012964:Sodium; D005947:Glucose