title: Tunicamycin-induced inhibition of protein secretion into culture medium of Arabidopsis T87 suspension cells through mRNA degradation on the endoplasmic reticulum.
abstract: The N-glycosylation inhibitor tunicamycin triggers endoplasmic reticulum stress response and inhibits efficient protein secretion in eukaryotes. Using Arabidopsis suspension cells, we showed that the reduced secretion of mannose-binding lectin 1 (MBL1) protein by tunicamycin is accompanied by a significant decrease in MBL1 mRNA, suggesting that mRNA destabilization is the major cause of the inhibition of protein secretion in plants.
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Osaka , Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Iwata
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Osaka , Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Hayashi
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Osaka , Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tabara
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Osaka , Japan.
authors.forename: Kei-Ichiro
authors.initials: K
authors.lastname: Mishiba
authors.affiliation: a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Osaka , Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Koizumi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D003470:Culture Media; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D015870:Gene Expression; D006031:Glycosylation; D037601:Mannose-Binding Lectin / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D059828:Plant Cells / Q000187:drug effects* / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D037601:Mannose-Binding Lectin; D012333:RNA, Messenger; D014415:Tunicamycin
keywords: Arabidopsis suspension cells; endoplasmic reticulum; mRNA degradation; protein secretion; tunicamycin