title: Comprehensive Genomic Analysis and Expression Profiling of the NOX Gene Families under Abiotic Stresses and Hormones in Plants.
abstract: Plasma membrane NADPH oxidases (NOXs) are key producers of reactive oxygen species under both normal and stress conditions in plants and they form functional subfamilies. Studies of these subfamilies indicated that they show considerable evolutionary selection. We performed a comparative genomic analysis that identified 50 ferric reduction oxidases (FRO) and 77 NOX gene homologs from 20 species representing the eight major plant lineages within the supergroup Plantae: glaucophytes, rhodophytes, chlorophytes, bryophytes, lycophytes, gymnosperms, monocots, and eudicots. Phylogenetic and structural analysis classified these FRO and NOX genes into four well-conserved groups represented as NOX, FRO I, FRO II, and FRO III. Further analysis of NOXs of phylogenetic and exon/intron structures showed that single intron loss and gain had occurred, yielding the diversified gene structures during the evolution of NOXs family genes and which were classified into four conserved subfamilies which are represented as Sub.I, Sub.II, Sub.III, and Sub.IV. Additionally, both available global microarray data analysis and quantitative real-time PCR experiments revealed that the NOX genes in Arabidopsis and rice (Oryza sativa) have different expression patterns in different developmental stages, various abiotic stresses and hormone treatments. Finally, coexpression network analysis of NOX genes in Arabidopsis and rice revealed that NOXs have significantly correlated expression profiles with genes which are involved in plants metabolic and resistance progresses. All these results suggest that NOX family underscores the functional diversity and divergence in plants. This finding will facilitate further studies of the NOX family and provide valuable information for functional validation of this family in plants.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Chang
authors.affiliation: Guangdong Academy of Agricultural Sciences, Argo-Biological Gene Research Center, Guangzhou, Guangdong, P. R. China.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Miao
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Shuai-Qi
authors.initials: SQ
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Ri
authors.initials: R
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, P. R. China.
authors.forename: Wen-Qiang
authors.initials: WQ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, P. R. China kunmingchen@nwsuaf.edu.cn kunmingchen@163.com.
authors.forename: Kun-Ming
authors.initials: KM
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D007438:Introns / Q000235:genetics; D005810:Multigene Family; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D017382:Reactive Oxygen Species; D009247:NADH, NADPH Oxidoreductases
keywords: NADPH oxidase (NOX); abiotic stress; coexpression; hormone; phylogenetic analysis