title: Chiral Nanozymes-Gold Nanoparticle-Based Transphosphorylation Catalysts Capable of Enantiomeric Discrimination.
abstract: Enantioselectivity in RNA cleavage by a synthetic metalloenzyme has been demonstrated for the first time. Thiols containing chiral Zn(II) -binding head groups have been self-assembled on the surface of gold nanoparticles. This results in the spontaneous formation of chiral bimetallic catalytic sites that display different activities (kcat ) towards the enantiomers of an RNA model substrate. Substrate selectivity is observed when the nanozyme is applied to the cleavage of the dinucleotides UpU, GpG, ApA, and CpC, and remarkable differences in reactivity are observed for the cleavage of the enantiomerically pure dinucleotide UpU.
authors.affiliation: Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131, Padova, Italy.
authors.forename: Jack L-Y
authors.initials: JL
authors.lastname: Chen
authors.affiliation: Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131, Padova, Italy.
authors.forename: Cristian
authors.initials: C
authors.lastname: Pezzato
authors.affiliation: Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131, Padova, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Scrimin
authors.affiliation: Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131, Padova, Italy. leonard.prins@unipd.it.
authors.forename: Leonard J
authors.identifier: 0000-0001-6664-822X
authors.initials: LJ
authors.lastname: Prins
mesh_terms: D001665:Binding Sites; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry*; D059367:RNA Cleavage; D013237:Stereoisomerism; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties; D015032:Zinc / Q000737:chemistry*
chemical_list: D009711:Nucleotides; D013438:Sulfhydryl Compounds; D012313:RNA; D006046:Gold; D015032:Zinc
keywords: chirality; enzyme mimics; nanozymes; self-assembly; transphosphorylation