title: [Expression of LXR-β in human gastric cancer tissue and the effect of GW3965 on the proliferation of gastric cancer cell line SGC-7901].
abstract: OBJECTIVE
  To examine the expression of liver X receptor-β (LXR-β) in human gastric cancer tissue, and to explore the effect of GW3965, an agonist of LXRs, on proliferation of gastric cancer cell line SGC-7901.
  
  
  METHODS
  The immunohistochemical assay was used to detect the expression of LXR-β, activating transcription factor 4 (ATF4) in gastric cancer tissues and the corresponding pericarcinoma tissues in 114 patients. Real-time quantitative PCR and Western blot were used to determine mRNA and protein levels of ATF4 and ATP-binding cassette 1 (ABCA1), one of the downstream target genes of LXRs, in SGC-7901 cells with or without GW3965 treatment. Cell counting kit-8 (CCK-8) assay was performed to detect cell proliferation. The expression of ATF4 was silenced by short hairpin RNA (shRNA).
  
  
  RESULTS
  The expressions of LXR-β and ATF-4 were obviously down-regulated in the gastric cancer tissues than that in the corresponding pericarcinoma tissues (both P<0.05). Compared with the control cells, GW3965 treatment inhibited proliferation of SGC-7901 cells and up-regulated ATF4 and ABCA1 expressions (both P<0.05). Knockdown of ATF4 can reverse the antiproliferative effect of GW3965 on SGC-7901 cells.
  
  
  CONCLUSIONS
  The expression of LXR-β is decreased in human gastric cancer tissues, and activation of LXRs by GW3965 could inhibit the proliferation of SGC-7901 cells via ATF4.
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Ruixin
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Qiaocheng
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Peng
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Xiangzhou
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhikang
authors.initials: Z
authors.lastname: Chen
mesh_terms: D051701:Activating Transcription Factor 4 / Q000235:genetics / Q000378:metabolism; D001565:Benzoates / Q000494:pharmacology*; D001596:Benzylamines / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000473:pathology*; D015854:Up-Regulation
chemical_list: C497109:ATF4 protein, human; D001565:Benzoates; D001596:Benzylamines; C473027:GW 3965; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051701:Activating Transcription Factor 4