title: A DNA topoisomerase VI-like complex initiates meiotic recombination.
abstract: The SPO11 protein catalyzes the formation of meiotic DNA double strand breaks (DSBs) and is homologous to the A subunit of an archaeal topoisomerase (topo VI). Topo VI are heterotetrameric enzymes comprising two A and two B subunits; however, no topo VIB involved in meiotic recombination had been identified. We characterized a structural homolog of the archaeal topo VIB subunit [meiotic topoisomerase VIB-like (MTOPVIB)], which is essential for meiotic DSB formation. It forms a complex with the two Arabidopsis thaliana SPO11 orthologs required for meiotic DSB formation (SPO11-1 and SPO11-2) and is absolutely required for the formation of the SPO11-1/SPO11-2 heterodimer. These findings suggest that the catalytic core complex responsible for meiotic DSB formation in eukaryotes adopts a topo VI-like structure.
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vrielynck
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Chambon
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Vezon
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Pereira
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Liudmila
authors.initials: L
authors.lastname: Chelysheva
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: De Muyt
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Mézard
authors.affiliation: Institut Pasteur, 25 rue du Dr Roux, 75015, Paris, France. CNRS, UMR 3528, 75724 Paris cedex 15, France. Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Claudine
authors.initials: C
authors.lastname: Mayer
authors.affiliation: Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France. mathilde.grelon@versailles.inra.fr.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Grelon
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics; D019843:Archaeal Proteins / Q000737:chemistry* / Q000235:genetics; D002384:Catalysis; D020134:Catalytic Domain; D053903:DNA Breaks, Double-Stranded; D026942:DNA Topoisomerases / Q000737:chemistry / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000737:chemistry* / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics; D059765:Homologous Recombination; D008540:Meiosis / Q000235:genetics*; D017020:Methanosarcina / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D016415:Sequence Alignment; D040681:Structural Homology, Protein; D016944:Sulfolobus / Q000201:enzymology*; D020798:Two-Hybrid System Techniques; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D029681:Arabidopsis Proteins; D019843:Archaeal Proteins; D026942:DNA Topoisomerases; D004250:DNA Topoisomerases, Type II; D004706:Endodeoxyribonucleases; D000099257:Meiotic Recombination Protein SPO11; C477460:Spo11-1 protein, Arabidopsis; C105141:DNA topoisomerase VI; C515046:SPO11-2 protein, Arabidopsis