title: The Aeromonas salmonicida Lipopolysaccharide Core from Different Subspecies: The Unusual subsp. pectinolytica.
abstract: Initial hydridization tests using Aeromonas salmonicida typical and atypical strains showed the possibility of different lipopolysaccharide (LPS) outer cores among these strains. By chemical structural analysis, LPS-core SDS-PAGE gel migration, and functional and comparative genomics we demonstrated that typical A. salmonicida (subsp. salmonicida) strains and atypical subsp. masoucida and probably smithia strains showed the same LPS outer core. A. salmonicida subsp. achromogenes strains show a similar LPS outer core but lack one of the most external residues (a galactose linked α1-6 to heptose), not affecting the O-antigen LPS linkage. A. salmonicida subsp. pectinolytica strains show a rather changed LPS outer core, which is identical to the LPS outer core from the majority of the A. hydrophila strains studied by genomic analyses. The LPS inner core in all tested A. salmonicida strains, typical and atypical, is well-conserved. Furthermore, the LPS inner core seems to be conserved in all the Aeromonas (psychrophilic or mesophilic) strains studied by genomic analyses.
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona Barcelona, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Merino
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona Barcelona, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Tomás
keywords: Aeromonas salmonicida; genomics; inner and outer core chemical structure; lipopolysaccharide core; typical and atypical