title: microRNA-34a-Upregulated Retinoic Acid-Inducible Gene-I Promotes Apoptosis and Delays Cell Cycle Transition in Cervical Cancer Cells.
abstract: The function of retinoic acid-inducible gene-I (RIG-I) in viral replication is well documented, but its function in carcinogenesis and malignancies as well as relationship with microRNAs (miRNAs) remain poorly understood. miR-34a is an antioncogene in multiple tumors. In our study, RIG-I and miR-34a suppressed cell growth, proliferation, migration, and invasion in cervical cancer cells in vitro. miR-34a was validated as a new regulator of RIG-I by binding to its 3' untranslated region and upregulating its expression level. Furthermore, we revealed that RIG-I and miR-34a enhanced apoptosis, delayed the G1/S/G2 transition of the cell cycle, and inhibited the epithelial-mesenchymal transition process to modulate malignancies in cervical cancer cells. Phenotypic rescue experiments indicated that RIG-I mediates the effects of miR-34a in HeLa and C33A cells. These findings provide new insights into the mechanisms that underlie carcinogenesis and may provide new biomarkers for the diagnosis and therapy of cervical cancer.
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University , Tianjin, China .
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University , Tianjin, China .
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University , Tianjin, China .
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Ma
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University , Tianjin, China .
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University , Tianjin, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University , Tianjin, China .
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University , Tianjin, China .
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D020413:3' Untranslated Regions; D000071457:DEAD Box Protein 58 / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D011971:Receptors, Immunologic; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D011971:Receptors, Immunologic; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58