title: Blood Transcriptomic Markers in Patients with Late-Onset Major Depressive Disorder.
abstract: We investigated transcriptomic markers of late-onset major depressive disorder (LOD; onset age of first depressive episode ≥ 50 years) from the genes expressed in blood cells and identified state-dependent transcriptomic markers in these patients. We assessed the genes expressed in blood cells by microarray and found that the expression levels of 3,066 probes were state-dependently changed in the blood cells of patients with LOD. To select potential candidates from those probes, we assessed the genes expressed in the blood of an animal model of depression, ovariectomized female mice exposed to chronic ultra-mild stress, by microarray and cross-matched the differentially expressed genes between the patients and the model mice. We identified 14 differentially expressed genes that were similarly changed in both patients and the model mice. By assessing statistical significance using real-time quantitative PCR (RT-qPCR), the following 4 genes were selected as candidates: cell death-inducing DFFA-like effector c (CIDEC), ribonuclease 1 (RNASE1), solute carrier family 36 member-1 (SLC36A1), and serine/threonine/tyrosine interacting-like 1 (STYXL1). The discriminating ability of these 4 candidate genes was evaluated in an independent cohort that was validated. Among them, CIDEC showed the greatest discriminant validity (sensitivity 91.3% and specificity 87.5%). Thus, these 4 biomarkers should be helpful for properly diagnosing LOD.
authors.affiliation: Departments of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Miyata
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kurachi
authors.affiliation: Departments of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Okano
authors.affiliation: Departments of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Sakurai
authors.affiliation: Division of Neuropsychiatry, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Kobayashi
authors.affiliation: Division of Neuropsychiatry, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Harada
authors.affiliation: Division of Neuropsychiatry, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Yamagata
authors.affiliation: Division of Neuropsychiatry, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Matsuo
authors.affiliation: Departments of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Departments of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Narita
authors.affiliation: Departments of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Fukuda
authors.affiliation: Department of Molecular and Cellular Neurobiology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Ishizaki
authors.affiliation: Departments of Psychiatry and Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Mikuni
mesh_terms: D017668:Age of Onset; D000368:Aged; D000818:Animals; D001522:Behavior, Animal; D015331:Cohort Studies; D003865:Major Depressive Disorder / Q000097:blood* / Q000235:genetics*; D004644:Emotions; D005260:Female; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D059467:Transcriptome
chemical_list: D005819:Genetic Markers