title: Methods for the Recognition of GAG-Bound Chemokines.
abstract: Chemokines play a pivotal role in the multistep cascade of cellular recruitment, where they provide the directional signal. They activate cells through a high-affinity interaction with their receptors, members of the large family of heptahelical G protein-coupled receptors. In order to provide the directional signal, they bind to cell surface proteoglycans through a low-affinity interaction with the glycosaminoglycan (GAG) moiety. While several methods have been described to measure the chemokine-GAG interaction, this chapter describes methods to identify whether anti-chemokine antibodies or chemokine-binding proteins recognize the GAG-bound chemokine.
authors.affiliation: NovImmune S.A., Geneva, Switzerland.
authors.forename: Pauline
authors.initials: P
authors.lastname: Bonvin
authors.affiliation: NovImmune S.A., Geneva, Switzerland.
authors.forename: Franck
authors.initials: F
authors.lastname: Gueneau
authors.affiliation: NovImmune S.A., Geneva, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fischer
authors.affiliation: NovImmune S.A., Geneva, Switzerland. Electronic address: amandapf@orange.fr.
authors.forename: Amanda
authors.initials: A
authors.lastname: Proudfoot
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D018925:Chemokines / Q000032:analysis* / Q000276:immunology / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006025:Glycosaminoglycans / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007368:Interferometry / Q000379:methods; D008967:Molecular Biology / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D018925:Chemokines; D006025:Glycosaminoglycans; D006493:Heparin
keywords: Chemokine; Chemokine inhibition; Chemokine-binding protein; Glycosaminoglycan; Monoclonal antibody