title: Bilateral Common Carotid Artery Occlusion in Spontaneously Hypertensive Rats: A Feasible Animal Model for Ocular Ischemic Syndrome.
abstract: The purpose of this study was to investigate the feasibility of inducing ocular ischemic syndrome in spontaneously hypertensive rats. Hypertensive and normotensive Wistar-Kyoto rats had bilateral occlusion or sham surgery. They were divided into 4 groups: (1) hypertensive-ischemia, (2) hypertensive-sham, (3) normotensive-ischemia, and (4) normotensive-sham. Four months after the operation, the global changes of the eye and pupillary light reflex were assessed. Then each rat was perfused, and randomly one of the bulbuses oculi was prepared as retinal flat mounts for investigation of vascular changes. The opposite eyeball was prepared as a paraffin section for observation of the linear density of retinal ganglion cells and for thickness measurement. One hypertensive-ischemia rat had a cataract in one eye and another rat in the same group had bulbus oculi collapse in one eye. The light reflex disappeared in 13.33% of hypertensive-ischemia rats, and the rest of the hypertensive-ischemia rats and normotensive-ischemia rats had slow reflex. Compared with the respective controls, the peripheral retinal vascular network in hypertensive-ischemia and normotensive-ischemia rats was sparse; linear density of the retinal ganglion cells was significantly reduced; and the retinal thickness was reduced. Compared with normotensive-ischemia rats, the hypertensive-ischemia rats demonstrated more severe changes. After bilateral common carotic artery occlusion, the eyes of hypertensive rats developed various pathological changes similar to those of ocular ischemic syndrome. In conclusion, an animal model for ocular ischemic syndrome can be created by bilateral common carotid artery occlusion in spontaneously hypertensive rats. Anat Rec, 299:806-814, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Ophthalmology, the Fourth Hospital of Shijiazhuang, Shijiazhuang, Hebei Province, 050011, China.
authors.forename: Yacong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, 510080, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050000, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Imaging and Interventional Radiology, Prince of Wales Hospital, the Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
authors.forename: Yi-Xiang J
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, the Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, Shijiazhuang, Hebei Province, 050000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Institute of Chinese Medicine, the Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Willmann
authors.initials: W
authors.lastname: Liang
authors.affiliation: Institute of Chinese Medicine, the Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Chinese Medicine, the Chinese University of Hong Kong, Hong Kong, China.
authors.forename: David
authors.initials: D
authors.lastname: T W Yew
authors.affiliation: Department of Ophthalmology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050000, China.
authors.forename: Cunxi
authors.initials: C
authors.lastname: Ye
authors.affiliation: Department of Medicine, Shenzhen University Health Science Centre, Shenzhen, 518060, China.
authors.forename: Ou
authors.initials: O
authors.lastname: Sha
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000473:pathology*; D016893:Carotid Stenosis / Q000473:pathology*; D004195:Disease Models, Animal; D005123:Eye / Q000098:blood supply*; D006973:Hypertension / Q000473:pathology*; D007511:Ischemia / Q000473:pathology*; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
keywords: bilateral common carotid artery occlusion; ocular ischemic syndrome; pupillary light reflex; retinal vessel pattern; spontaneously hypertensive rat