title: Neutron diffraction analysis of Pseudomonas aeruginosa peptidyl-tRNA hydrolase 1.
abstract: Perdeuterated peptidyl-tRNA hydrolase 1 from Pseudomonas aeruginosa was crystallized for structural analysis using neutron diffraction. Crystals of perdeuterated protein were grown to 0.15 mm(3) in size using batch crystallization in 22.5% polyethylene glycol 4000, 100 mM Tris pH 7.5, 10%(v/v) isopropyl alcohol with a 20-molar excess of trilysine as an additive. Neutron diffraction data were collected from a crystal at room temperature using the MaNDi single-crystal diffractometer at Oak Ridge National Laboratory.
authors.affiliation: Department of Chemistry, University of Alabama in Huntsville, 301 Sparkman Drive, Huntsville, AL 35899, USA.
authors.forename: Hana
authors.initials: H
authors.lastname: McFeeters
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831, USA.
authors.forename: Venu Gopal
authors.initials: VG
authors.lastname: Vandavasi
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831, USA.
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Weiss
authors.affiliation: Biology and Soft Matter Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831, USA.
authors.forename: Leighton
authors.initials: L
authors.lastname: Coates
authors.affiliation: Department of Chemistry, University of Alabama in Huntsville, 301 Sparkman Drive, Huntsville, AL 35899, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: McFeeters
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D033363:Neutron Diffraction; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase
keywords: Pseudomonas aeruginosa; antibiotic resistance; neutron diffraction; peptidyl-tRNA hydrolase 1; perdeuteration