title: Targeting Fibronectin To Disrupt In Vivo Candida albicans Biofilms.
abstract: New drug targets are of great interest for the treatment of fungal biofilms, which are routinely resistant to antifungal therapies. We theorized that the interaction of Candida albicans with matricellular host proteins would provide a novel target. Here, we show that an inhibitory protein (FUD) targeting Candida-fibronectin interactions disrupts biofilm formation in vitro and in vivo in a rat venous catheter model. The peptide appears to act by blocking the surface adhesion of Candida, halting biofilm formation.
authors.affiliation: Department of Medicine, University of Wisconsin, Madison, Wisconsin, USA Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, USA.
authors.forename: Jeniel E
authors.initials: JE
authors.lastname: Nett
authors.affiliation: Department of Medicine, University of Wisconsin, Madison, Wisconsin, USA Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cabezas-Olcoz
authors.affiliation: Department of Medicine, University of Wisconsin, Madison, Wisconsin, USA Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Marchillo
authors.affiliation: Department of Medicine, University of Wisconsin, Madison, Wisconsin, USA Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin, USA.
authors.forename: Deane F
authors.initials: DF
authors.lastname: Mosher
authors.affiliation: Department of Medicine, University of Wisconsin, Madison, Wisconsin, USA Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, USA dra@medicine.wisc.edu.
authors.forename: David R
authors.initials: DR
authors.lastname: Andes
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D055499:Catheter-Related Infections / Q000382:microbiology; D005353:Fibronectins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D051381:Rats
chemical_list: D000935:Antifungal Agents; D005353:Fibronectins