title: Localized lentivirus delivery via peptide interactions.
abstract: Gene delivery from biomaterial scaffolds has been employed to induce the expression of tissue inductive factors for applications in regenerative medicine. The delivery of viral vectors has been described as reflecting a balance between vector retention and release. Herein, we investigated the design of hydrogels in order to retain the vector at the material in order to enhance transgene expression. Poly(ethylene-glycol) (PEG) hydrogels were modified with poly-l-lysine (PLL) to non-covalently bind lentivirus. For cells cultured on the hydrogels, increasing the PLL molecular weight from 1 to 70 kDa led to increased transgene expression. The incubation time of the virus with the hydrogel and the PLL concentration modulated the extent of virus adsorption, and adsorbed virus had a 20% increase in the half-life at 37°C. Alternatives to high molecular weight PLL were identified through phage display technology, with peptide sequences specific for the VSV-G ectodomain, an envelope protein pseudotyped on the virus. These affinity peptides could easily be incorporated into the hydrogel, and expression was increased 20-fold relative to control peptide, and comparable to levels observed with the high molecular weight PLL. The modification of hydrogels with affinity proteins or peptides to bind lentivirus can be a powerful strategy to enhance and localized transgene expression. Biotechnol. Bioeng. 2016;113: 2033-2040. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan.
authors.forename: Michael
authors.initials: M
authors.lastname: Skoumal
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, Los Angeles, California.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Seidlits
authors.affiliation: Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois.
authors.forename: Seungjin
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan. ldshea@umich.edu.|Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd, 1119 Gerstacker, Ann Arbor, Michigan, 48109. ldshea@umich.edu.
authors.forename: Lonnie
authors.initials: L
authors.lastname: Shea
mesh_terms: D018014:Gene Transfer Techniques; D057809:HEK293 Cells; D006801:Humans; D020100:Hydrogels; D016086:Lentivirus / Q000235:genetics* / Q000378:metabolism; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry
chemical_list: D020100:Hydrogels; D019151:Peptide Library; D010455:Peptides; D011107:Polylysine
keywords: lentivirus; localized gene delivery; phage display; polylysine