title: Differential Expression of O-glycoprotein Glycans in Cholangiocarcinoma Cell Lines.
abstract: Protein glycosylation is the most common posttranslational modification in mammalian cells. Aberrant protein glycosylation has been reported in various diseases, including cancer. We identified and quantified the glycan structures of O-linked glycoprotein from cholangiocarcinoma (CCA) cell lines from different histological types and compared their profiles by nanospray ionization-linear ion trap mass spectrometry (NSI-MSn). Five human CCA cell lines, K100, M055, M139, M213 and M214 were characterized. The results showed that the O-linked glycans of the CCA cell lines comprised tri- to hexa-saccharides with terminal galactose and sialic acids: NeuAc1Gal1GalNAc1, Gal2GlcNAc1GalNAc1, NeuAc2Gal1GalNAc1 NeuAc1Gal2GlcNAc1GalNAc1 and NeuAc2Gal2GlcNAc1GalNAc1 All five CCA cell lines showed a similar glycan pattern, but with differences in their quantities. NeuAc1Gal1GalNAc1 proved to be the most abundant structure in poorly differentiated adenocarcinoma (K100; 57.1%), moderately differentiated adenocarcinoma (M055; 42.6%) and squamous cell carcinoma (M139; 43.0%), while moderately to poorly differentiated adenocarcinoma (M214; 40.1%) and adenosquamous cell carcinoma (M213; 34.7%) appeared dominated by NeuAc2Gal1GalNAc1. These results demonstrate differential expression of the O-linked glycans in the different histological types of CCA. All five CCA cell lines have abundant terminal sialic acid (NeuAc) O-linked glycans, suggesting an important role for sialic acid in cancer cells. Our structural analyses of glycans may provide important information regarding physiology of disease-related glycoproteins in CCA.
authors.affiliation: School of Pathology, Institute of Medicine, Nakhon Ratchasima, Thailand, E-mail : krajang.t@sut.ac.th.
authors.forename: Krajang
authors.initials: K
authors.lastname: Talabnin
authors.forename: Chutima
authors.initials: C
authors.lastname: Talabnin
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishihara
authors.forename: Parastoo
authors.initials: P
authors.lastname: Azadi
authors.forename: Sopit
authors.initials: S
authors.lastname: Wongkham
authors.forename: Banchob
authors.initials: B
authors.lastname: Sripa
mesh_terms: D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D011134:Polysaccharides / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D006023:Glycoproteins; D011134:Polysaccharides