title: Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype.
abstract: A Micropallet-Array-based strategy allowing the identification of cells of defined phenotype in complex mixtures, such as would be obtained from a tissue biopsy, is presented. Following the distribution of single adherent cells from the mixture on individual pedestals, termed "micropallets", immunofluorescent confocal imaging is applied to interrogate the expression of five cell surface molecules.
authors.affiliation: Department of Molecular Biology and Biochemistry, Ayala School of Biological Sciences, University of California at Irvine, 839 Medical Sciences Ct., b100c Sprague Hall, Irvine, CA, 92697, USA.
authors.forename: Trisha M
authors.initials: TM
authors.lastname: Westerhof
authors.affiliation: Department of Electrical Engineering and Computer Science, Samueli School of Engineering, Department of Biomedical Engineering, Samueli School of Engineering, Department of Chemical Engineering and Materials Science, Samueli School of Engineering, University of California at Irvine, 4100 Calit2 building, Irvine, CA, 92697, USA.
authors.forename: Guann-Pyng
authors.initials: GP
authors.lastname: Li
authors.affiliation: Department of Electrical Engineering and Computer Science, Samueli School of Engineering, Department of Biomedical Engineering, Samueli School of Engineering, University of California at Irvine, 2300 Engineering Gateway, Irvine, CA, 92697, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Bachman
authors.affiliation: Department of Molecular Biology and Biochemistry, Ayala School of Biological Sciences, University of California at Irvine, 839 Medical Sciences Ct., b100c Sprague Hall, Irvine, CA, 92697, USA.|Department of Medicine, School of Medicine, University of California, Irvine Medical Center, 101 The City Drive, Building 56, Room 247, Orange, CA, 92868, USA.
authors.forename: Edward L
authors.initials: EL
authors.lastname: Nelson
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional; D010641:Phenotype; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes
keywords: biomems; immunofluorescent confocal microscopy; micropallet array; single cell analysis