title: Inhibition of HL-60 cell growth via cell cycle arrest and apoptosis induction by a cycloartane-labdane heterodimer from Pseudolarix amabilis.
abstract: Pseudolaridimer C (), a rarely encountered cycloartane-labdane Diels-Alder adduct was isolated from the cones of Pseudolarix amabilis. The structure and absolute configuration of were established by comprehensive NMR and CD spectral analysis. The WST-8 assay indicated that time and dose dependently inhibited the proliferation of human leukemia cells HL-60 at 1-10 μM. DAPI and Annexin V-FITC/PI double staining method, and DNA ladder experiments all proved that had significant dose-dependent effects on HL-60 cell apoptosis. A further mechanism study indicated that the apoptosis was associated with the cell cycle arrest during the G2/M phase, and the activation of caspase-9, -3, -7, and poly-(ADP-ribose)-polymerase (PARP).
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Niao
authors.initials: N
authors.lastname: Yang
authors.affiliation: Innovative Research Center of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 201204, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Xike
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Rongcai
authors.initials: R
authors.lastname: Yue
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Huiliang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com.
authors.forename: Yunheng
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China. wdzhangy@hotmail.com shenyunheng@hotmail.com and Innovative Research Center of Traditional Chinese Medicine, Shanghai Institute of Pharmaceutical Industry, Shanghai 201204, P. R. China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D019281:Dimerization; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D008968:Molecular Conformation; D028201:Pinaceae / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D014315:Triterpenes; C433017:labdane; C085352:cycloartane