title: Dissolution DNP for in vivo preclinical studies.
abstract: The tremendous polarization enhancement afforded by dissolution dynamic nuclear polarization (DNP) can be taken advantage of to perform preclinical in vivo molecular and metabolic imaging. Following the injection of molecules that are hyperpolarized via dissolution DNP, real-time measurements of their biodistribution and metabolic conversion can be recorded. This technology therefore provides a unique and invaluable tool for probing cellular metabolism in vivo in animal models in a noninvasive manner. It gives the opportunity to follow and evaluate disease progression and treatment response without requiring ex vivo destructive tissue assays. Although its considerable potential has now been widely recognized, hyperpolarized magnetic resonance by dissolution DNP remains a challenging method to implement for routine in vivo preclinical measurements. The aim of this article is to provide an overview of the current state-of-the-art technology for preclinical applications and the challenges that need to be addressed to promote it and allow its wider dissemination in the near future.
authors.affiliation: General Electric Healthcare, Pollards Wood, Nightingales Lane, Chalfont St Giles, Buckinghamshire HP8 4SP, United Kingdom; Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. Electronic address: arnaud.comment@ge.com.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Comment
mesh_terms: D000818:Animals; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008660:Metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012995:Solubility
keywords: Dynamic nuclear polarization; Hyperpolarization; MRI; Magnetic resonance; Molecular imaging