title: Evaluation of the Activity of the Essential Oil from an Ornamental Flower against Aedes aegypti: Electrophysiology, Molecular Dynamics and Behavioral Assays.
abstract: Dengue fever has spread worldwide and affects millions of people every year in tropical and subtropical regions of Africa, Asia, Europe and America. Since there is no effective vaccine against the dengue virus, prevention of disease transmission depends entirely on regulating the vector (Aedes aegypti) or interrupting human-vector contact. The aim of this study was to assess the oviposition deterrent activity of essential oils of three cultivars of torch ginger (Etlingera elatior, Zingiberaceae) against the dengue mosquito. Analysis of the oils by gas chromatography (GC)-mass spectrometry revealed the presence of 43 constituents, of which α-pinene, dodecanal and n-dodecanol were the major components in all cultivars. Solutions containing 100 ppm of the oils exhibited oviposition deterrent activities against gravid Ae. aegypti females. GC analysis with electroantennographic detection indicated that the oil constituents n-decanol, 2-undecanone, undecanal, dodecanal, trans-caryophyllene, (E)-β-farnesene, α-humulene, n-dodecanol, isodaucene and dodecanoic acid were able to trigger antennal depolarization in Ae. aegypti females. Bioassays confirmed that solutions containing 50 ppm of n-dodecanol or dodecanal exhibited oviposition deterrent activities, while a solution containing the alcohol and aldehyde in admixture at concentrations representative of the oil presented an activity similar to that of the 100 ppm oil solution. Docking and molecular dynamics simulations verified that the interaction energies of the long-chain oil components and Ae. aegypti odorant binding protein 1 were quite favorable, indicating that the protein is a possible oviposition deterrent receptor in the antenna of Ae. aegypti.
authors.affiliation: Laboratório de Ecologia Química, Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
authors.forename: Patrícia C
authors.initials: PC
authors.lastname: Bezerra-Silva
authors.affiliation: Laboratório de Ecologia Química, Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
authors.forename: Kamilla A
authors.initials: KA
authors.lastname: Dutra
authors.affiliation: Laboratório de Ecologia Química, Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
authors.forename: Geanne K N
authors.initials: GK
authors.lastname: Santos
authors.affiliation: Laboratório de Ecologia Química, Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
authors.forename: Rayane C S
authors.initials: RC
authors.lastname: Silva
authors.affiliation: Laboratório de Purificação e Determinação de Estruturas de Proteínas, Departamento de Química, Universidade Estadual de Ponta Grossa, 84070-900, Ponta Grossa, PR, Brazil.
authors.forename: Jorge
authors.initials: J
authors.lastname: Iulek
authors.affiliation: Laboratório de Ecologia Química, Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Milet-Pinheiro
authors.affiliation: Laboratório de Ecologia Química, Departamento de Química Fundamental, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
authors.forename: Daniela M A F
authors.initials: DM
authors.lastname: Navarro
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000818:Animals; D057506:Arthropod Antennae / Q000187:drug effects / Q000502:physiology; D001522:Behavior, Animal / Q000187:drug effects*; D003715:Dengue / Q000635:transmission; D055724:Electrophysiological Phenomena / Q000187:drug effects*; D005260:Female; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism; D007303:Insect Vectors / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D056004:Molecular Dynamics Simulation; D009822:Oils, Volatile / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011487:Protein Conformation; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D019476:Insect Proteins; D009822:Oils, Volatile