title: Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1.
abstract: In this study, the nitrate transporter gene CmNRT1 was isolated from the chrysanthemum variety 'Nannongxuefeng'. The full-length cDNA contains an open reading frame of 1761 bp encoding 587 residues. Using qRT-PCR, we found that CmNRT1 was induced by 10 mM NO3(-) in roots and shoots. Two Arabidopsis thaliana transgenic plants expressing CmNRT1 were selected for functional analyses. Root (15)N influx in wild-type and transgenic A. thaliana lines under 10 or 0.2 mM (15)NO3 was tested. Our results indicate that CmNRT1 encodes a constitutive component for a low-affinity transporter.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: C S
authors.initials: CS
authors.lastname: Gu
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: F D
authors.initials: FD
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D027321:Anion Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics; D031202:Chrysanthemum / Q000378:metabolism*; D008969:Molecular Sequence Data; D000091285:Nitrate Transporters; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D030821:Plants, Genetically Modified; D016415:Sequence Alignment
chemical_list: D027321:Anion Transport Proteins; D000091285:Nitrate Transporters; D010940:Plant Proteins