title: Selective and Sensitive Chemiluminescence Determination of MCPB: Flow Injection and Liquid Chromatography.
abstract: Two new chemiluminescence (CL) methods are described for the determination of the herbicide 4-(4-chloro-o-tolyloxy) butyric acid (MCPB). First, a flow injection chemiluminescence (FI-CL) method is proposed. In this method, MCPB is photodegraded with an ultraviolet (UV) lamp and the photoproducts formed provide a great CL signal when they react with ferricyanide in basic medium. Second, a high-performance liquid chromatography chemiluminescence (HPLC-CL) method is proposed. In this method, before the photodegradation and CL reaction, the MCPB and other phenoxyacid herbicides are separated in a C18 column. The experimental conditions for the FI-CL and HPLC-CL methods are optimized. Both methods present good sensitivity, the detection limits being 0.12 µg L(-1) and 0.1 µg L(-1) (for FI-CL and HPLC-CL, respectively) when solid phase extraction (SPE) is applied. Intra- and interday relative standard deviations are below 9.9%. The methods have been satisfactorily applied to the analysis of natural water samples. FI-CL method can be employed for the determination of MCPB in simple water samples and for the screening of complex water samples in a fast, economic, and simple way. The HPLC-CL method is more selective, and allows samples that have not been resolved with the FI-CL method to be solved.
authors.affiliation: Instituto de Investigación para la Gestión Integrada de Zonas Costeras. Universitat Politècnica de València, Valencia, Spain sumello@qim.upv.es.
authors.forename: Susana
authors.initials: S
authors.lastname: Meseguer-Lloret
authors.affiliation: Instituto de Investigación para la Gestión Integrada de Zonas Costeras. Universitat Politècnica de València, Valencia, Spain.
authors.forename: Sagrario
authors.initials: S
authors.lastname: Torres-Cartas
authors.affiliation: Instituto de Investigación para la Gestión Integrada de Zonas Costeras. Universitat Politècnica de València, Valencia, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Catalá-Icardo
authors.affiliation: Instituto de Investigación para la Gestión Integrada de Zonas Costeras. Universitat Politècnica de València, Valencia, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Gómez-Benito
mesh_terms: D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D002087:Butyrates / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008163:Luminescent Measurements; D052616:Solid Phase Extraction / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D002087:Butyrates; D014874:Water Pollutants, Chemical; D008456:2-Methyl-4-chlorophenoxyacetic Acid; C008418:2-methyl-4-chlorophenoxy gamma-butyric acid
keywords: Chemiluminescence; Flow injection; HPLC; Liquid chromatography; High-performance liquid chromatography; MCPB; Photoreactor; SPE; Solid phase extraction