title: An Analytical Pipeline for Quantitative Characterization of Dietary Intake: Application To Assess Grape Intake.
abstract: Lack of accurate dietary assessment in free-living populations requires discovery of new biomarkers reflecting food intake qualitatively and quantitatively to objectively evaluate effects of diet on health. We provide a proof-of-principle for an analytical pipeline to identify quantitative dietary biomarkers. Tartaric acid was identified by nuclear magnetic resonance spectroscopy as a dose-responsive urinary biomarker of grape intake and subsequently quantified in volunteers following a series of 4-day dietary interventions incorporating 0 g/day, 50 g/day, 100 g/day, and 150 g/day of grapes in standardized diets from a randomized controlled clinical trial. Most accurate quantitative predictions of grape intake were obtained in 24 h urine samples which have the strongest linear relationship between grape intake and tartaric acid excretion (r(2) = 0.90). This new methodological pipeline for estimating nutritional intake based on coupling dietary intake information and quantified nutritional biomarkers was developed and validated in a controlled dietary intervention study, showing that this approach can improve the accuracy of estimating nutritional intakes.
authors.affiliation: Nutrition and Dietetic Research Group, Division of Endocrinology and Metabolism, Imperial College London , London W12 0NN, U.K.
authors.forename: Isabel
authors.initials: I
authors.lastname: Garcia-Perez
authors.affiliation: Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: Joram M
authors.initials: JM
authors.lastname: Posma
authors.affiliation: Nutrition and Dietetic Research Group, Division of Endocrinology and Metabolism, Imperial College London , London W12 0NN, U.K.
authors.forename: Edward S
authors.initials: ES
authors.lastname: Chambers
authors.affiliation: Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: Jeremy K
authors.initials: JK
authors.lastname: Nicholson
authors.affiliation: Human Nutrition Research Centre, Institute of Cellular Medicine, Newcastle University , Newcastle upon Tyne NE4 5PL, U.K.
authors.forename: John
authors.initials: J
authors.lastname: C Mathers
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University , Aberystwyth SY23 3DA, U.K.
authors.forename: Manfred
authors.initials: M
authors.lastname: Beckmann
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University , Aberystwyth SY23 3DA, U.K.
authors.forename: John
authors.initials: J
authors.lastname: Draper
authors.affiliation: Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: Elaine
authors.initials: E
authors.lastname: Holmes
authors.affiliation: Nutrition and Dietetic Research Group, Division of Endocrinology and Metabolism, Imperial College London , London W12 0NN, U.K.
authors.forename: Gary
authors.initials: G
authors.lastname: Frost
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine*; D004032:Diet; D005260:Female; D005638:Fruit / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013644:Tartrates / Q000008:administration & dosage / Q000652:urine*; D027843:Vitis
chemical_list: D015415:Biomarkers; D013644:Tartrates; C029768:tartaric acid
keywords: accurate dietary assessment; metabolic profiling; nutritional intake; quantified dietary biomarkers; tartaric acid