title: Sysmex XT-2000iV scattergram analysis in a cat with basophilia.
abstract: A 13-year-old female Domestic Shorthair cat was presented to the Veterinary Teaching Hospital of the University of Milan for an interscapular mass suspected to be a mesenchymal malignant tumor. A preoperative CBC performed with Sysmex XT-2000iV showed leukocytosis with neutrophilia and eosinophilia. The Sysmex WBC/DIFF scattergram showed an additional, well-separated cluster of events between the neutrophil, eosinophil, and lymphocyte clusters. Blood smear evaluation revealed the presence of a significant number of basophils; thus, it was hypothesized that the additional cluster could represent the basophilic population. A second CBC, 24 days later, showed the same pattern on the WBC/DIFF scattergram in the absence of leukocytosis and neutrophilia. After surgical excision of the mass, a definitive diagnosis of feline injection site sarcoma was made. To the author's knowledge, there are no previous reports about the identification of feline basophils in the WBC/DIFF scattergram of Sysmex XT-2000iV.
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Angelica
authors.initials: A
authors.lastname: Stranieri
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Ferrari
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Sergio
authors.identifier: http://orcid.org/0000-0002-5741-4023
authors.initials: S
authors.lastname: Zanzani
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.|School of Veterinary and Life Science, Murdoch University, Murdoch, WA, Australia.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Rossi
keywords: Differential leukocyte count; feline blood; laser counter; lysis-resistant granulocytes