title: Isolation and characterization of a novel type of rotavirus species A in sugar gliders (Petaurus breviceps).
abstract: To estimate the risk of interspecies transmission of rotavirus species A (RVA) from exotic pets to other mammalian species, the prevalence of RVA in sugar gliders (Petaurus breviceps) was investigated. RVAs were detected in 10 of 44 sugar gliders by reverse transcription (RT)-semi-nested PCR. These viruses were classified as G27P[3] and G27P[36] genotypes, with G27 and P[36] being new genotypes as assigned by the Rotavirus Classification Working Group. To characterize sugar glider RVA in detail, one strain, RVA/SugarGlider-tc/JPN/SG385/2012/G27P[36] (SG385-tc), was isolated. All of the genes of the strain were classified as new genotypes (G27-P[36]-I19-R10-C10-M9-A20-N11-T13-E17-H12). The enterotoxin domain in NSP4, which is important for the induction of diarrhoea, was conserved between SG385-tc and previously reported mammalian strains, suggesting the potential of sugar glider RVA to cause diarrhoea in mammalian species. In fact, seven out of nine suckling mice inoculated orally with 3.9 × 104 f.f.u. of strain SG385-tc had diarrhoea and the 50 % diarrhoea-inducing dose (DD50) of strain SG385-tc in suckling mice was 1.2 × 104 f.f.u. Our findings suggest that sugar glider RVA is infective to and possibly pathogenic in other mammalian species.
authors.affiliation: The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Okadera
authors.affiliation: The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Abe
authors.affiliation: The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.|Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Ito
authors.affiliation: The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Mitake
authors.affiliation: The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Okada
authors.affiliation: The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine,Azabu University, 1-17-71 Fuchinobe, Kanagawa, 252-5201,Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Une
authors.affiliation: Dairy Hygiene Research Division, National Institute of Animal Health,4 Hitsujigaoka, Hokkaido, 062-0045,Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
authors.affiliation: The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.|Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugiyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D036022:Capsid Proteins / Q000235:genetics; D005243:Feces / Q000821:virology; D005260:Female; D008394:Marsupialia / Q000821:virology*; D051379:Mice; D010802:Phylogeny; D011247:Pregnancy; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus