title: Molecular detection of chicken parvovirus in broilers with enteric disorders presenting curving of duodenal loop, pancreatic atrophy, and mesenteritis.
abstract: Enteric disorders are an important cause of economic losses in broiler chickens worldwide. Several agents have been associated with enteric problems, such as viruses, bacteria, and parasites. In this study, broiler chickens showing signs of enteric disorders were subjected to molecular diagnosis for several viral agents and also for pathological examination for elucidating this problem. Thus, the chickens were screened for avian nephritis virus (ANV), chicken astrovirus (CAstV), avian rotavirus (ArtV), avian reovirus (AReoV), infectious bronchitis virus (IBV), fowl adenovirus group I (FAdV-1), and chicken parvovirus (ChPV). Postmortem examinations revealed a curving of the duodenal loop (J-like appearance) and intestines filled with liquid and gaseous content. Histopathological analysis of the duodenal loop showed pancreatic atrophy, acute mesenteritis, and enteritis. PCR results showed that ChPV was the sole viral agent detected in samples with lesions such as the curved duodenal loop and pancreatic atrophy. Molecular characterization of the nucleotide and deduced amino acid sequences revealed a high similarity with other strains of ChPV from Brazil, Canada, United States, Europe, and Asia. These findings suggest an association between ChPV and the development of enteritis, pancreatitis, and pancreatic atrophy, which may lead to curling of the duodenal loop. Together, these alterations may disrupt the normal functioning of the digestive system, diminishing digestion and the absorption of dietary nutrients and consequently leading to reduced weight gain, flock impairment, dwarfism, and an elevated feed conversion rate.
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, 05508-900, São Paulo, Brazil.
authors.forename: L F N
authors.initials: LF
authors.lastname: Nuñez
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, 05508-900, São Paulo, Brazil.
authors.forename: L R M
authors.initials: LR
authors.lastname: Sá
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, 05508-900, São Paulo, Brazil.
authors.forename: S H S
authors.initials: SH
authors.lastname: Parra
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, 05508-900, São Paulo, Brazil.
authors.forename: C S
authors.initials: CS
authors.lastname: Astolfi-Ferreira
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, 05508-900, São Paulo, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Carranza
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, 05508-900, São Paulo, Brazil ajpferr@usp.br.
authors.forename: A J P
authors.initials: AJ
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D001284:Atrophy / Q000473:pathology / Q000662:veterinary; D002645:Chickens; D004386:Duodenum / Q000473:pathology*; D008286:Malabsorption Syndromes / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D010179:Pancreas / Q000473:pathology*; D010322:Parvoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D019219:Parvovirinae / Q000235:genetics / Q000502:physiology*; D011201:Poultry Diseases / Q000473:pathology* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
keywords: LD/MAS; chicken; mesenteritis; pancreatic atrophy; parvovirus