title: Experimental assessment of the pathogenicity of the Newcastle disease viruses from outbreaks in Great Britain in 1997 for chickens and turkeys, and the protection afforded by vaccination.
abstract: The Newcastle disease virus isolated from healthy turkeys in outbreak GB 97/6 was used to challenge 4-week-old turkeys and chickens, which were either not vaccinated or had received a single dose of Hitchner B1 live vaccine 14 days earlier, by one of the intramuscular, intranasal or contact routes. Similar experiments were done in 38-day-old turkeys and chickens using virus isolated from severely sick chickens in outbreak GB 97/1. All vaccinated chickens showed low but measurable immune responses 14 days after vaccination, but only three of the turkeys had detectable antibodies. No vaccinated turkey or chicken showed any clinical sign after challenge with either virus. The virus from healthy turkeys in outbreak GB 97/6 induced clinical signs in 12/30 unvaccinated turkeys after challenge and 7/30 died. In unvaccinated chickens, challenge with this virus produced clinical signs in 25/30 birds and 21/30 died. In challenge experiments with the virus from outbreak GB 97/1 in chickens, 3/30 unvaccinated turkeys showed clinical signs and all three subsequently died. In contrast, 30/30 unvaccinated chickens challenged with this virus showed clinical signs and died. Vaccination did not prevent infection and excretion of either challenge virus. However, when compared with unvaccinated birds, vaccination reduced significantly the length of time virus was excreted and the overall proportion of swabs that were positive.
authors.forename: D J
authors.initials: DJ
authors.lastname: Alexander
authors.forename: R J
authors.initials: RJ
authors.lastname: Manvell
authors.forename: J
authors.initials: J
authors.lastname: Banks
authors.forename: M S
authors.initials: MS
authors.lastname: Collins
authors.forename: G
authors.initials: G
authors.lastname: Parsons
authors.forename: B
authors.initials: B
authors.lastname: Cox
authors.forename: K M
authors.initials: KM
authors.lastname: Frost
authors.forename: E C
authors.initials: EC
authors.lastname: Speidel
authors.forename: S
authors.initials: S
authors.lastname: Ashman
authors.forename: E W
authors.initials: EW
authors.lastname: Aldous