title: Mycoavidus cysteinexigens gen. nov., sp. nov., an endohyphal bacterium isolated from a soil isolate of the fungus Mortierella elongata.
abstract: An endohyphal bacterium (strain B1-EBT) living in association with the fungus Mortierella elongata FMR23-6 I-B1 was isolated from a fungal cell homogenate and studied for its taxonomic allocation. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, non-motile, and negative for oxidase and catalase. Strain B1-EBT required cysteine for growth and grew at temperatures between 4 and 35 °C. A comparative analysis of 16S rRNA gene sequences revealed that strain B1-EBT forms a distinct clade in the family Burkholderiaceae, encompassing a group of endosymbionts associated with several soil isolates of M. elongata. The most closely related genus is 'Candidatus Glomeribacter gigasporarum', an endosymbiont of the arbuscular mycorrhizal fungus Gigaspora margarita. The major cellular fatty acids of strain B1-EBT were C16 : 0, summed feature 3 (C16 : 1ω7c and C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c or C18 : 1ω6c). Ubiquinone Q-8 was the only quinone detected. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid and two unknown aminolipids. The DNA G+C content was 49.8 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic characteristics, strain B1-EBT represents a novel genus and novel species in the family Burkholderiaceae, for which the name Mycoavidus cysteinexigens gen. nov., sp. nov. is proposed. The type strain is B1-EBT ( = JCM 30646T = LMG 28693T = NBRC 110909T).
authors.affiliation: Ibaraki University College of Agriculture,3-21-1 Chuo, Ami-machi, Ibaraki 300-0393,Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Ohshima
authors.affiliation: National Research Institute for Cultural Properties,Tokyo, 13-43 Ueno-park, Taito-ku, Tokyo 110-8713,Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Atmosphere and Ocean Research Institute, The University of Tokyo,5-1-5, Kashiwanoha, Kashiwa-shi, Chiba 277-8564,Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Fujimura
authors.affiliation: Ibaraki University College of Agriculture,3-21-1 Chuo, Ami-machi, Ibaraki 300-0393,Japan.|United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology,3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509,Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takashima
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC),2-5-8 Kazusakamatari, Kisarazu-shi, Chiba 292-0818,Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Ibaraki University College of Agriculture,3-21-1 Chuo, Ami-machi, Ibaraki 300-0393,Japan.
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Nishizawa
authors.affiliation: Ibaraki University College of Agriculture,3-21-1 Chuo, Ami-machi, Ibaraki 300-0393,Japan.|United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology,3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509,Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Narisawa
authors.affiliation: Ibaraki University College of Agriculture,3-21-1 Chuo, Ami-machi, Ibaraki 300-0393,Japan.|United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology,3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509,Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohta
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D042521:Burkholderiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D020094:Mortierella; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8