title: Enhanced butanol production from cassava with Clostridium acetobutylicum by genome shuffling.
abstract: To obtain strains exhibiting high levels of solvent tolerance and butanol production, wild type strains of Clostridium acetobutylicum butanol-producing strain GX01 and Lactobacillus mucosae butanol-tolerant strain M26 were subjected to mutagenesis combining N-methyl-N-nitro-N-nitrosoguanidine induction with genome shuffling. After four successive rounds of genome shuffling, the C. acetobutylicum shuffled strain GS4-3 showing greater levels of fermentation performances (such as secreting a higher level of amylase, improving the thermal stability, and possessing greater environmental robustness) compared to the wild type strains was isolated. As a result, after optimization of culture conditions, mutant GS4-3 produced 32.6 g/L of total solvent, 20.1 g/L of butanol production, and 0.35 g/L/h of butanol productivity, which were, respectively, increased by 23.5, 23.3, and 40.0 % than the wild-type strain GX01, in a 10 L bioreactor. The enhanced production of butanol and tolerance of solvent of mutant associated with GS4-3 make it promising for acetone/butanol/ethanol fermentation from cassava (Manihot esculenta).
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China.
authors.forename: Shu-Bo
authors.initials: SB
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qian
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
authors.forename: Zheng-Wu
authors.initials: ZW
authors.lastname: Liang
authors.affiliation: National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning, 530007, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China.
authors.forename: Mou-Ming
authors.initials: MM
authors.lastname: Zhao
authors.affiliation: College of Life Science and Technology, Guangxi University, Nanning, 530004, China. pangzw@gxu.edu.cn.
authors.forename: Zong-Wen
authors.initials: ZW
authors.lastname: Pang
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D019149:Bioreactors; D046969:Clostridium acetobutylicum / Q000235:genetics* / Q000254:growth & development; D032621:DNA Shuffling / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development; D002365:Manihot / Q000737:chemistry*; D016296:Mutagenesis; D009604:Nitrosoguanidines / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D009604:Nitrosoguanidines; D020001:1-Butanol
keywords: Butanol; Cassava; Clostridium acetobutylicum; Genome shuffling; N-methyl-N-nitro-N-nitrosoguanidine mutation