title: DNA barcoding reveals mislabeling of imported fish products in Nansha new port of Guangzhou, Guangdong province, China.
abstract: In the present study, we employed a DNA barcoding approach to authenticate the species of fish imported via one port in China. The fish were identified as smallmouth scad based on morphological characteristics, Alepes apercna (Perciformes, Carangidae), but were labeled as Rastrelliger brachysoma (Perciformes, Scombridae). Fragments of the partial mitochondrial cytochrome c oxidase 1 (COI) gene were sequenced from 12 specimens, and their phylogenetic relationship was subsequently examined. The phylogenetic analysis demonstrated that all of the individuals formed a monophyletic cluster with high bootstrap values and were placed in a sister group with the ancestor of Alepes vari and Alepes melanoptera. The K2P genetic distances at an intraspecific level were significantly smaller than those at an interspecific level. Our results indicated that the fish were A. apercna, rather than R. brachysoma, which confirms the morphological analysis. This study presents a practical demonstration of the use of DNA barcoding to prevent fraud in international trade.
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yan
authors.affiliation: Nansha Entry-Exit Inspection and Quarantine Bureau, Guangzhou 511457, PR China.
authors.forename: Guiyan
authors.initials: G
authors.lastname: Lai
authors.affiliation: Nansha Entry-Exit Inspection and Quarantine Bureau, Guangzhou 511457, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Nansha Entry-Exit Inspection and Quarantine Bureau, Guangzhou 511457, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Nansha Entry-Exit Inspection and Quarantine Bureau, Guangzhou 511457, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Nansha Entry-Exit Inspection and Quarantine Bureau, Guangzhou 511457, PR China; Guangdong Institute of Standardization, Guangzhou 510220, PR China. Electronic address: wangm58@aliyun.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics; D005396:Fish Products; D005515:Food Labeling; D010473:Perciformes / Q000145:classification* / Q000235:genetics; D010802:Phylogeny
chemical_list: D003576:Electron Transport Complex IV
keywords: Alepes apercna; COI; DNA barcoding; Mislabeling; Species identification