title: Activation of TLR5 induces podocyte apoptosis.
abstract: The apoptosis plays a critical role in a number of inflammatory disorders. Bacterial infection is one of the causes inducing apoptosis. This study aims to investigate the mechanism by which activation of TLR5 induces podocyte apoptosis. In this study, a podocyte cell line was cultured in RPMI1640 medium. The expression of TLR5 was assessed by real-time PCR and Western blotting. The Fas ligand gene transcription was assessed by immunoprecipitation and chromatin immunoprecipitation assay. The results showed that the expression of TLR5 was observed in the podocytes at both mRNA and protein levels. Exposure to TLR5 ligand, flagellin, induced Fas ligand expression and podocyte apoptosis. p300, one of the histone acetyltransferases, mediated the Fas ligand gene transcription in podocytes. In conclusion, TLR5 activation plays an important role in the induction of podocyte apoptosis.
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Haiting
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Yanwu
authors.initials: Y
authors.lastname: You
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Chunrong
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D005408:Flagellin / Q000494:pharmacology; D008024:Ligands; D051379:Mice; D050199:Podocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051198:Toll-Like Receptor 5 / Q000235:genetics / Q000378:metabolism*
chemical_list: D008024:Ligands; D051198:Toll-Like Receptor 5; D005408:Flagellin
keywords: Fas ligand; TLR5; apoptosis; flagellin; kidney; podocyte