title: Living cells of probiotic Bifidobacterium bifidum YIT 10347 detected on gastric mucosa in humans.
abstract: The probiotic strain Bifidobacterium bifidum YIT 10347 has been demonstrated to inhibit Helicobacter pylori activity, prevent injury to the gastric mucosa, and improve general gastric malaise symptoms in H. pylori positive patients. This study aimed to investigate the adhering activity and localisation of B. bifidum YIT 10347 to gastric cells and tissue in vitro, and in human in vivo to clarify the mechanism of its beneficial effects on the stomach. The in vitro study found the adhesion rate of B. bifidum YIT 10347 to human gastric epithelial cells was about 10 times higher than that of lactic acid bacteria and other bifidobacteria. In the human study, 5 H. pylori negative and 12 H. pylori positive subjects ingested milk fermented with B. bifidum YIT 10347. B. bifidum YIT 10347 cells were measured by RT-qPCR for in gastric biopsy samples. Living B. bifidum YIT 10347 cells were detected in the biopsy samples in H. pylori negative subjects (105 cells/g and 104 cells/g at 1 h and 2 h after ingestion, respectively) and H. pylori positive subjects (104 cells/g at 1 h after the ingestion). Moreover, immunostaining analysis of tissue sections found that B. bifidum YIT 10347 cells were located at the interstitial mucin layer of the stomach. These results suggest that cells of probiotic B. bifidum YIT 10347 adhered to the human gastric mucosa in a live state, and that the higher adhering activity of B. bifidum YIT 10347 to the gastric mucosa may be involved in its beneficial effects on the human stomach.
authors.affiliation: 1 Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shibahara-Sone
authors.affiliation: 1 Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Gomi
authors.affiliation: 1 Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iino
authors.affiliation: 1 Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kano
authors.affiliation: 2 Faculty of Research and Development, Yakult Honsha, 1-19 Higashi Shimbashi, Minato-ku, Tokyo 105-8660, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Nonaka
authors.affiliation: 2 Faculty of Research and Development, Yakult Honsha, 1-19 Higashi Shimbashi, Minato-ku, Tokyo 105-8660, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Watanabe
authors.affiliation: 1 Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
authors.affiliation: 3 Department of Internal Medicine, Kashiwa Hospital, The Jikei University School of Medicine, 163-1 Kashiwashita, Kashiwa, Chiba 277-8567, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohkusa
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion; D058491:Bacterial Load; D000069985:Bifidobacterium bifidum / Q000302:isolation & purification* / Q000502:physiology; D001706:Biopsy; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D064368:Healthy Volunteers; D016481:Helicobacter Infections / Q000473:pathology / Q000628:therapy; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D050296:Microbial Viability; D008875:Middle Aged; D019936:Probiotics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction
keywords: Heliobacter pylori; Lactobacillus bifidum; fermented milk; immunostaining; probiotics