title: Delivery System of CpG Oligodeoxynucleotides through Eliciting an Effective T cell Immune Response against Melanoma in Mice.
abstract: OBJECTIVE
  In order to improve the immunogenicity of whole tumor cell lysate for tumor vaccine, we have designed a series of CpG ODNs to study their transport and to evaluate their anti-tumor activity in B16 melanoma mouse models.
  
  
  METHODS
  In this study, we investigated whether C-class CpG ODN (CpG ODN-685) could facilitate tumor cell lysate to induce vigorous anti-tumor activity against tumors in mice both prophylactically and therapeutically.
  
  
  RESULTS
  It was found that the combination of tumor cell lysate and CpG ODN-685 could inhibit the growth of B16 melanoma and prolong the survival of tumor-bearing mice. Moreover CpG ODN-685 with the addition of tumor cell lysate can also cause the generation of tumor specific immune memory by inducing specific cytotoxic T lymphocytes and helper T lymphocytes in mice.
  
  
  CONCLUSIONS
  The results suggest that CpG ODN-685 could be developed as an efficient adjuvant for tumor vaccines against melanoma.
authors.affiliation: 1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: 1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Fang
authors.affiliation: 1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 3. NSF Nanoscale Science and Engineering Center (NSEC), The Ohio State University, Columbus, OH 43212, USA.
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: 1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: 1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wan
authors.affiliation: 1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: 2. Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.affiliation: 1. Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
keywords: CpG ODN; T lymphocytes; melanoma; tumor cell lysate