title: Evaluation of cephamycins as supplements to selective agar for detecting Campylobacter spp. in chicken carcass rinses.
abstract: Although cefoperazone is the most commonly used antibiotic in Campylobacter-selective media, the distribution of cefoperazone-resistant bacteria such as extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli is increasing. Here we evaluated the potential of cephamycins for use as supplements to improve modified charcoal-cefoperazone-deoxycholate agar (mCCDA) by replacing cefoperazone with the same concentrations (32 mg/L) of cefotetan (modified charcoal-cefotetan-deoxycholate agar, mCCtDA) and cefoxitin (modified charcoal-cefoxitin-deoxycholate agar, mCCxDA). In chicken carcass rinse samples, the number of mCCDA plates detecting for Campylobacter (18/70, 26%) was significantly lower than that of mCCtDA (42/70, 60%) or mCCxDA plates (40/70, 57%). The number of mCCDA plates (70/70, 100%) that were contaminated with non-Campylobacter species was significantly higher than that of mCCtDA (20/70, 29%) or mCCxDA plates (21/70, 30%). The most common competing species identified using mCCDA was ESBL-producing E. coli, while Pseudomonas species frequently appeared on mCCtDA and mCCxDA.
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Young-Ji
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Food & Nutrition College of Human Ecology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea.
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Kim
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea.
authors.forename: Kwang-Young
authors.initials: KY
authors.lastname: Song
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
authors.forename: Kidon
authors.initials: K
authors.lastname: Sung
authors.affiliation: Center for One Health, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea. Electronic address: bracstu3@konkuk.ac.kr.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000362:Agar / Q000737:chemistry*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D002167:Campylobacter / Q000187:drug effects*; D002513:Cephamycins / Q000494:pharmacology*; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D002513:Cephamycins; D003470:Culture Media; D000362:Agar
keywords: Campylobacter; Cefotetan; Cefoxitin; Chicken; ESBL