title: Curcumin induced apoptosis via PI3K/Akt-signalling pathways in SKOV3 cells.
abstract: Context Curcumin is widely used in China and India as a traditional herb but additional work is required to ascertain the folkloric claim of its antitumour and antioxidant activities. Objective The present study determines the antitumour effect of curcumin against SKOV3 cell growth. Materials and methods SKOV3 cells were incubated with curcumin (0, 20, 30 and 40 μM) for 72 h. The antiproliferative activity and the apoptosis rate were measured by MTT and flow cytometry. Expression of PI3K, T-Akt and p-Akt proteins was measured by western blotting. Results The administration of curcumin (0, 20, 30 and 40 μM) inhibits SKOV3 cell growth (IC50  value= 24.8 μM) and increased apoptosis (32.5 and 85.7%). The activity of SKOV3 cell invasion (98.2 and 19.4%) was also decreased by curcumin administration (p < 0.05). Results of western blot analysis confirmed that the expression of p-Akt protein was decreased by curcumin (p < 0.05). It was also found that a high dose of curcumin (40 μM) can cause stronger antitumour activity (80.4%). Conclusion Our results suggest that the curcumin induced SKOV3 apoptosis via modulation of the PI3K/Akt-signalling pathway.
authors.affiliation: a Department of Oncology , Binzhou Medical University Hospital , Shandong , China ;
authors.forename: Zeshun
authors.initials: Z
authors.lastname: Yu
authors.affiliation: b Department of Oncology , Binzhou PEOPLE'S Hospital , Shandong , China ;
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wan
authors.affiliation: c Department of Gynecology , Binzhou Medical University Hospital , Shandong , China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Department of Oncology , Binzhou Medical University Hospital , Shandong , China ;
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: a Department of Oncology , Binzhou Medical University Hospital , Shandong , China ;
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: a Department of Oncology , Binzhou Medical University Hospital , Shandong , China ;
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt; D003474:Curcumin
keywords: Antitumour; MTT; medicinal plant