title: Clerodane-type diterpenoids from tuberous roots of Tinospora sagittata (Oliv.) Gagnep.
abstract: Three new clerodane type diterpenoids (2-4), together with one known analogues (1), were isolated from the tuberous roots of Tinospora sagittata (Oliv.) Gagnep. Compound 3 is an unusual clerodane diterpenoid with the carbonyl functionality at C-3, which represents the first example from this diterpenoid compounds class. The structures were established on the basis of spectroscopic data interpretation. The absolute configuration of 1 was determined for the first time by single-crystal X-ray diffraction analysis with CuKα irradiation. These isolates were evaluated for their cytotoxic activities against five human cancer cell lines and inhibitory activities on LPS-induced NO production in RAW264.7 cells.
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China.
authors.forename: Song
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China.
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Zhejiang 310053, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China.
authors.forename: Quanwei
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Jinwen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Wuhan First Hospital, Wuhan 430022, Hubei, China. Electronic address: wangfuqian.c@163.com.
authors.forename: Fuqian
authors.initials: F
authors.lastname: Wang
authors.affiliation: Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: zhangyh@mails.tjmu.edu.cn.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor / Q000187:drug effects; D018360:Crystallography, X-Ray; D045785:Diterpenes, Clerodane / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D000067996:RAW 264.7 Cells / Q000187:drug effects; D031610:Tinospora / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane; D010936:Plant Extracts
keywords: Cytotoxicity; Diterpenoid; Nitric oxide; Tinospora sagittata