title: Proteome Dynamics of the Specialist Oxalate DegraderOxalobacter formigenes.
abstract: Oxalobacter formigenesis a unique intestinal organism that relies on oxalate degradation to meet most of its energy and carbon needs. A lack of colonization is a risk factor for calcium oxalate kidney stone disease. The release of the genome sequence ofO. formigeneshas provided an opportunity to increase our understanding of the biology ofO. formigenes. This study used mass spectrometry based shotgun proteomics to examine changes in protein levels associated with the transition of growth from log to stationary phase. Of the 1867 unique protein coding genes in the genome ofO. formigenesstrain OxCC13, 1822 proteins were detected, which is at the lower end of the range of 1500-7500 proteins found in free-living bacteria. From the protein datasets presented here it is clear thatO. formigenescontains a repertoire of metabolic pathways expected of an intestinal microbe that permit it to survive and adapt to new environments. Although further experimental testing is needed to confirm the physiological and regulatory processes that mediate adaptation with nutrient shifts, theO. formigenesprotein datasets presented here can be used as a reference for studying proteome dynamics under different conditions and have significant potential for hypothesis development.
authors.affiliation: Department of Urology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Melissa E
authors.initials: ME
authors.lastname: Ellis
authors.affiliation: Department of Urology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Mobley
authors.affiliation: Department of Urology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Ross P
authors.initials: RP
authors.lastname: Holmes
authors.affiliation: Department of Urology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: John
authors.initials: J
authors.lastname: Knight
keywords: Calcium oxalate stone disease; Oxalobacter formigenes; Proteome dynamics