title: Profiling the genome-wide DNA methylation pattern of porcine ovaries using reduced representation bisulfite sequencing.
abstract: Substantial evidence has shown that DNA methylation regulates the initiation of ovarian and sexual maturation. Here, we investigated the genome-wide profile of DNA methylation in porcine ovaries at single-base resolution using reduced representation bisulfite sequencing. The biological variation was minimal among the three ovarian replicates. We found hypermethylation frequently occurred in regions with low gene abundance, while hypomethylation in regions with high gene abundance. The DNA methylation around transcriptional start sites was negatively correlated with their own CpG content. Additionally, the methylation level in the bodies of genes was higher than that in their 5' and 3' flanking regions. The DNA methylation pattern of the low CpG content promoter genes differed obviously from that of the high CpG content promoter genes. The DNA methylation level of the porcine ovary was higher than that of the porcine intestine. Analyses of the genome-wide DNA methylation in porcine ovaries would advance the knowledge and understanding of the porcine ovarian methylome.
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Yuan
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Gao
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Zhang
authors.affiliation: College of Biological and Food Engineering, Guangdong University of Education, Guangzhou, Guangdong, China.
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: He
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Guangzhou Ribobio Co., Ltd., Guangzhou, Guangdong, China.
authors.forename: Wen-Mian
authors.initials: WM
authors.lastname: Lin
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Zan-Mou
authors.initials: ZM
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Centre for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Li
mesh_terms: D000818:Animals; D018899:CpG Islands; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D010053:Ovary / Q000502:physiology*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D013552:Swine
chemical_list: D004247:DNA