title: T cell-specific inactivation of mouse CD2 by CRISPR/Cas9.
abstract: The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific promoters can edit genes in the mouse and may thus allow targeted studies of gene function in vivo.
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Germany.|Institute of Laboratory Animal Science, University of Zurich, Schlieren, Switzerland.
authors.forename: Jane
authors.initials: J
authors.lastname: Beil-Wagner
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Dössinger
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Germany.
authors.forename: Kilian
authors.initials: K
authors.lastname: Schober
authors.affiliation: Institute of Laboratory Animal Science, University of Zurich, Schlieren, Switzerland.
authors.forename: Johannes
authors.initials: J
authors.lastname: vom Berg
authors.affiliation: Max-Planck-Institute for Plant Breeding Research, Cologne, Germany.|Department of Biology, Albertus-Magnus University, Cologne, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Tresch
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Grandl
authors.affiliation: Institute of Laboratory Animal Science, University of Zurich, Schlieren, Switzerland.
authors.forename: Pushpalatha
authors.initials: P
authors.lastname: Palle
authors.affiliation: Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Mair
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Gerhard
authors.affiliation: Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Becher
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Germany.
authors.forename: Dirk H
authors.initials: DH
authors.lastname: Busch
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Germany.|Institute of Laboratory Animal Science, University of Zurich, Schlieren, Switzerland.
authors.forename: Thorsten
authors.initials: T
authors.lastname: Buch
mesh_terms: D000818:Animals; D001483:Base Sequence; D018801:CD2 Antigens / Q000235:genetics* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D064113:CRISPR-Cas Systems; D000072669:Gene Editing / Q000379:methods*; D020868:Gene Silencing; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D011401:Promoter Regions, Genetic; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D018801:CD2 Antigens; D000094704:RNA, Guide, CRISPR-Cas Systems