title: [Transmembrane prostate androgen-induced protein 1 (PMEPA1) promotes the migration and maintains mesenchymal-like phenotype of breast cancer cells].
abstract: OBJECTIVE
  To investigate the role of transmembrane prostate androgen-induced protein 1 (PMEPA1), an important gene downstream of transforming growth factor-β (TGF-β) signaling, in the process of breast cancer cell migration and epithelial-mesenchymal transition.
  
  
  METHODS
  We treated MDA-MB-231 breast cancer cells with TGF-β and TGF-β inhibitor SB431542, and then detect the level of PMEPA1 using Western blotting. PMEPA1-specific siRNA was designed and its knockdown efficiency was tested by quantitative real-time PCR (qRT-PCR). After the expression of PMEPA1 in MDA-MB-231 cells was successfully silenced, the wound-healing assay and Transwell(TM) assay were used to investigate the effect of PMEPA1 silencing on the migration of MDA-MB-231 cells. Moreover, phalloidin was used to label the actin cytoskeleton of breast cancer cells to observe the effect of PMEPA1 silencing on cell morphology.
  
  
  RESULTS
  In breast cancer cells, PMEPA1 was upregulated by classical TGF-β/Smad signaling pathway. Silencing of PMEPA1 significantly inhibited the migration ability of MDA-MB-231 cells and promoted the process of mesenchymal-epithelial transition.
  
  
  CONCLUSIONS
  Over-expressed PMEPA1 can promote cell migration and maintain the mesenchymal-like morphology of breast cancer cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Student Brigade, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yuankang
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Angang
authors.initials: A
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: jialth@fmmu.edu.cn.
authors.forename: Lintao
authors.initials: L
authors.lastname: Jia
authors.affiliation: Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: rnawangl@fmmu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D001549:Benzamides / Q000494:pharmacology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D004149:Dioxoles / Q000494:pharmacology; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D034622:RNA Interference; D018125:Receptors, Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects
chemical_list: C459179:4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide; D001549:Benzamides; D004149:Dioxoles; D008565:Membrane Proteins; C412868:PMEPA1 protein, human; D018125:Receptors, Transforming Growth Factor beta