title: Secondary metabolites from the flower buds of Lonicera japonica and their in vitro anti-diabetic activities.
abstract: Four new compounds (1, 2, 7 and 8) and twenty known compounds were isolated from the flower buds of Lonicera japonica. Their structures were determined by extensive NMR and HR-ESIMS spectroscopic data analyses. Among them, compounds 1 and 2 are a pair of diastereoisomers possessing a rare chemical structure, and their absolute configurations were determined by comparing their experimental and calculated ECD spectra. Furthermore, all the isolates were evaluated for their inhibitory effects on α-glucosidase and protein tyrosine phosphatase 1B (PTP1B), especially 1 and 2, which displayed both significant inhibitions. In addition, the possible action mechanism of the active compounds was also explored by using molecular docking studies.
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Zhuoyang
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: He
authors.affiliation: School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.affiliation: College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, People's Republic of China.
authors.forename: Pinyi
authors.initials: P
authors.lastname: Gao
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address: songsj99@163.com.
authors.forename: Shaojiang
authors.initials: S
authors.lastname: Song
mesh_terms: D035264:Flowers / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification; D031239:Lonicera / Q000737:chemistry*; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors; D064210:Secondary Metabolism; D000520:alpha-Glucosidases
chemical_list: D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C516607:PTPN1 protein, human; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D000520:alpha-Glucosidases
keywords: ECD; Lonicera japonica; Molecular docking; PTP1B; α-Glucosidase