title: An improved classification of stationary phases for ultra-high performance supercritical fluid chromatography.
abstract: Supercritical fluid chromatography (SFC) has recently benefited of new instrumentation, together with the availability of many ultra-high performance columns (sub -2μm fully porous particles or sub -3μm superficially porous particles), rendering it more attractive than ever. Most of these columns commonly used in SFC were initially developed for HPLC use, with an increasing number of stationary phases specifically designed for SFC. While the availability of different stationary phase chemistries is an advantage to achieve successful SFC separations, selecting a column for method development remains difficult. For this reason, we have previously developed a classification of stationary phases dedicated to SFC use. It is based on linear solvation energy relationships (LSER) with Abraham descriptors (for neutral species). While current interest in SFC is strong in the pharmaceutical industry, the need to take account of interactions occurring with ionisable species is pressing. We have previously shown how a modified version of the solvation parameter model, adapted to take account of ionic and ionizable species, could be applied to the characterization of SFC systems. In the present paper, based on this modified LSER model, and on the analysis of 109 neutral and ionisable species, we propose an improved classification of 31 ultra-high performance stationary phases to facilitate method development with SFC.
authors.affiliation: Univ Orleans, CNRS, Institut de Chimie Organique et Analytique (ICOA), UMR 7311 B.P. 6759, rue de Chartres, F-45067 Orléans cedex 2, France. Electronic address: caroline.west@univ-orleans.fr.
authors.forename: Caroline
authors.initials: C
authors.lastname: West
authors.affiliation: Univ Orleans, CNRS, Institut de Chimie Organique et Analytique (ICOA), UMR 7311 B.P. 6759, rue de Chartres, F-45067 Orléans cedex 2, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Lemasson
authors.affiliation: Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Bertin
authors.affiliation: Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Hennig
authors.affiliation: Univ Orleans, CNRS, Institut de Chimie Organique et Analytique (ICOA), UMR 7311 B.P. 6759, rue de Chartres, F-45067 Orléans cedex 2, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Lesellier
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid; D007477:Ions; D016062:Porosity; D011312:Pressure
chemical_list: D007477:Ions
keywords: Ionic interactions; Method development; Selectivity; Solvation parameter model; Stationary phases; Supercritical fluid chromatography