title: Overexpression of AQP5 Was Detected in Axillary Sweat Glands of Primary Focal Hyperhidrosis Patients.
abstract: BACKGROUND
  The expression of aquaporin 5 (AQP5) in human axillary sweat glands has never been studied so far.
  
  
  OBJECTIVE
  To detect the expression of AQP5 in axillary sweat glands of patients with primary focal hyperhidrosis (PFH) relative to control subjects.
  
  
  METHODS
  The morphological characteristics and the number of sweat coils in axillary sweat glands were compared between two groups by using transmission electron microscopy. The expression of AQP5 was detected by immunohistochemistry, Western blot analysis, and real-time transcription polymerase chain reaction.
  
  
  RESULTS
  There were no significant differences between the two groups in terms of morphological characteristics and the number of sweat coils in axillary sweat glands. The expressions of AQP5 protein and AQP5 mRNA were significantly higher in the patient group than in the control group.
  
  
  CONCLUSIONS
  AQP5 is involved in the secretion of human axillary sweat glands. The overexpression of AQP5 in sweat glands is probably one pathogenetic mechanism underlying PFH.
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou, PR China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.forename: Ju-Hua
authors.initials: JH
authors.lastname: Yang
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Chen
authors.forename: Yuan-Rong
authors.initials: YR
authors.lastname: Tu
mesh_terms: D000293:Adolescent; D000328:Adult; D051402:Aquaporin 5 / Q000032:analysis* / Q000235:genetics; D001365:Axilla; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006945:Hyperhidrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D046529:Microscopy, Electron, Transmission; D012333:RNA, Messenger / Q000032:analysis*; D013545:Sweat Glands / Q000737:chemistry* / Q000648:ultrastructure; D055815:Young Adult
chemical_list: C496036:AQP5 protein, human; D051402:Aquaporin 5; D012333:RNA, Messenger