title: Biotransformation studies of cresol red by Absidia spinosa M15.
abstract: Cresol Red, a commercial dye that used widely to color nylon, wool, cotton, and polyacrylonitrile-modified nylon in the massive textile manufacture is toxic recalcitrant. Absidia spinosa M15, a novel fungal strain isolated from a tropical rain forest, was found to decolorize Cresol Red 65% within 30 d under agitation condition. UV-Vis spectroscopy, TLC analysis and mass spectra of samples after decolorization process in culture medium confirmed final decolorization of Cresol Red. Two metabolites were identified in the treated medium: benzeneacetic acid (tR 9.6 min and m/z 136) and benzoic acid (tR 5.7 min and m/z 122). Laccase showed the significant activity (133.8 U/L) in biomass obtained at the end of experiment demonstrates role of the enzyme in the decolorization process.
authors.affiliation: Faculty of Engineering Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia.
authors.forename: Risky Ayu
authors.initials: RA
authors.lastname: Kristanti
authors.affiliation: Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
authors.forename: Meor Mohd
authors.initials: MM
authors.lastname: Fikri Ahmad Zubir
authors.affiliation: Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia; Department of Environmental Engineering, Faculty of Civil Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: hadibarata@utm.my.
authors.forename: Tony
authors.initials: T
authors.lastname: Hadibarata
mesh_terms: D020095:Absidia / Q000378:metabolism*; D019817:Benzoic Acid / Q000378:metabolism; D001673:Biodegradation, Environmental; D001711:Biotransformation; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D042845:Laccase / Q000378:metabolism; D010637:Phenolsulfonphthalein / Q000031:analogs & derivatives* / Q000378:metabolism; D010648:Phenylacetates / Q000378:metabolism; D065947:Rainforest; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D004396:Coloring Agents; D010648:Phenylacetates; D014874:Water Pollutants, Chemical; C009743:Cresol red; D019817:Benzoic Acid; D042845:Laccase; D010637:Phenolsulfonphthalein
keywords: Absidia spinosa M15; Cresol red; Decolorization; Metabolites; Triphenylmethane dye