title: Alkali-based pretreatments distinctively extract lignin and pectin for enhancing biomass saccharification by altering cellulose features in sugar-rich Jerusalem artichoke stem.
abstract: Jerusalem artichoke (JA) has been known as a potential nonfood feedstock for biofuels. Based on systems analysis of total 59 accessions, both soluble sugar and ash could positively affect biomass digestibility after dilute sodium hydroxide pretreatment (A). In this study, one representative accession (HEN-3) was used to illustrate its enzymatic digestibility with pretreatments of ultrasonic-assisted dilute sodium hydroxide (B), alkaline peroxide (C), and ultrasonic-assisted alkaline peroxide (D). Pretreatment D exhibited the highest hexose release rate (79.4%) and total sugar yield (10.4 g/L), which were 2.4 and 2.6 times higher, respectively, than those of the control. The analysis of cellulose crystalline index (CrI), cellulose degree of polymerization (DP), thermal behavior and SEM suggested that alkali-based pretreatments could distinctively extract lignin and pectin polymers, leading to significant alterations of cellulose CrI and DP for high biomass saccharification. Additionally, hydrogen peroxide (H2O2) could significant reduce the generation of fermentation inhibitors during alkali-based pretreatments.
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; National Energy R&D Center for Non-food Biomass, China Agricultural University, Beijing 100193, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; National Energy R&D Center for Non-food Biomass, China Agricultural University, Beijing 100193, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; National Energy R&D Center for Non-food Biomass, China Agricultural University, Beijing 100193, China.
authors.forename: Yuezhou
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; National Energy R&D Center for Non-food Biomass, China Agricultural University, Beijing 100193, China. Electronic address: xiegh@mail.cau.edu.cn.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Xie
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000468:Alkalies / Q000737:chemistry; D056804:Biofuels / Q000032:analysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000032:analysis / Q000737:chemistry*; D006368:Helianthus / Q000737:chemistry*; D006601:Hexoses / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D008031:Lignin / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry; D014465:Ultrasonics / Q000379:methods
chemical_list: D000468:Alkalies; D056804:Biofuels; D006601:Hexoses; D012972:Sodium Hydroxide; D010368:Pectins; D002482:Cellulose; D008031:Lignin; D006861:Hydrogen Peroxide; D019342:Acetic Acid
keywords: Alkali-based pretreatments; Cellulose CrI; Cellulose DP; Fermentation inhibitor; Helianthus tuberosus L.