title: Get1p and Get2p are required for maintenance of mitochondrial morphology and normal cardiolipin levels.
abstract: Cardiolipin (CL) is the signature phospholipid of mitochondrial membranes. CL deficiency leads to defects in mitochondrial function. Using a targeted synthetic lethality screen to identify defects that exacerbate CL deficiency, we determined that deletion of mitochondrial morphology genes in cells lacking CL leads to severe growth defects. We show that ER membrane proteins Get1p and Get2p are required for maintaining normal levels of CL. We propose that these proteins regulate the level of CL by maintaining wild type-like tubular mitochondrial morphology. The genetic interactions observed in this study identify novel physiological modifiers that are required for maintenance of CL levels and mitochondrial morphology.
authors.affiliation: Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Amit S
authors.initials: AS
authors.lastname: Joshi
authors.affiliation: Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
authors.forename: Naomi
authors.initials: N
authors.lastname: Fei
authors.affiliation: Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA mgreenberg@wayne.edu.
authors.forename: Miriam L
authors.initials: ML
authors.lastname: Greenberg
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism*; D017353:Gene Deletion; D005820:Genetic Testing; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D050296:Microbial Viability; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D002308:Cardiolipins; C503510:GET1 protein, S cerevisiae; C515526:Get2 protein, S cerevisiae; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: Barth syndrome; cardiolipin; mitochondria morphology