title: Engineering of soybean mosaic virus as a versatile tool for studying protein-protein interactions in soybean.
abstract: Transient gene expression approaches are valuable tools for rapid introduction of genes of interest and characterization of their functions in plants. Although agroinfiltration is the most effectively and routinely used method for transient expression of multiple genes in various plant species, this approach has been largely unsuccessful in soybean. In this study, we engineered soybean mosaic virus (SMV) as a dual-gene delivery vector to simultaneously deliver and express two genes in soybean cells. We further show the application of the SMV-based dual vector for a bimolecular fluorescence complementation assay to visualize in vivo protein-protein interactions in soybean and for a co-immunoprecipitation assay to identify cellular proteins interacting with SMV helper component protease. This approach provides a rapid and cost-effective tool for transient introduction of multiple traits into soybean and for in vivo characterization of the soybean cellular protein interaction network.
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Wanju 565-851, Republic of Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
mesh_terms: D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017800:Potyvirus / Q000235:genetics*; D060066:Protein Interaction Maps / Q000235:genetics*; D013025:Glycine max / Q000235:genetics* / Q000821:virology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus