title: Longitudinal imaging of the availability of dopamine transporter and D2 receptor in rat striatum following mild ischemia.
abstract: The changes in the availability of striatal dopamine transporter and dopamine D2 receptor after mild focal ischemia in rats were measured using a small animal positron emission tomography system. Mild focal ischemia was induced by 20-minute middle cerebral artery occlusion. [11C]PE2I binding to dopamine transporter was transiently increased on the ipsilateral side of the striatum at 2 days after middle cerebral artery occlusion. On day 7 and 14 after middle cerebral artery occlusion, [11C]PE2I binding levels were decreased. In contrast, [11C]raclopride binding to dopamine D2 receptor in the ipsilateral striatum had not changed at 2 days after middle cerebral artery occlusion. [11C]Raclopride binding was significantly decreased on the ischemic side of the striatum at 7 and 14 days after middle cerebral artery occlusion. Moreover, on day 1 and 2 after middle cerebral artery occlusion, significant circling behavior to the contralateral direction was induced by amphetamine challenge. This behavior disappeared at 7 days after middle cerebral artery occlusion. At 14 days, circling behavior to the ipsilateral direction (middle cerebral artery occlusion side) was significantly increased, and that to the contralateral direction also appeared again. The present study suggested that amphetamine-induced circling behavior indicated striatal dopaminergic alterations and that dopamine transporter and dopamine D2 receptor binding could be key markers for predicting motor dysfunction after mild focal ischemia.
authors.affiliation: 1 Department of Drug Metabolism & Pharmacokinetics, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Sotaro
authors.initials: S
authors.lastname: Momosaki
authors.affiliation: 1 Department of Drug Metabolism & Pharmacokinetics, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Ito
authors.affiliation: 2 Department of Applied Chemistry & Analysis, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Yamato
authors.affiliation: 2 Department of Applied Chemistry & Analysis, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iimori
authors.affiliation: 2 Department of Applied Chemistry & Analysis, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Sumiyoshi
authors.affiliation: 2 Department of Applied Chemistry & Analysis, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Morimoto
authors.affiliation: 1 Department of Drug Metabolism & Pharmacokinetics, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Imamoto
authors.affiliation: 4 Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Osaka, Japan.|5 PET Molecular Imaging Center, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Watabe
authors.affiliation: 3 Department of Molecular Imaging in Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.|4 Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Osaka, Japan.|5 PET Molecular Imaging Center, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Eku
authors.initials: E
authors.lastname: Shimosegawa
authors.affiliation: 4 Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Osaka, Japan.|5 PET Molecular Imaging Center, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Hatazawa
authors.affiliation: 1 Department of Drug Metabolism & Pharmacokinetics, Shionogi & Co., Ltd., Osaka, Japan.|3 Department of Molecular Imaging in Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Abe
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000000981:diagnostic imaging / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000032:analysis / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery / Q000000981:diagnostic imaging / Q000378:metabolism*; D008297:Male; D049268:Positron-Emission Tomography; D020891:Raclopride / Q000378:metabolism; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000032:analysis / Q000378:metabolism*
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D017448:Receptors, Dopamine D2; D020891:Raclopride
keywords: Middle cerebral artery occlusion; circling behavior; dopamine D2 receptor; dopamine transporter; positron emission tomography