title: Centlein, a novel microtubule-associated protein stabilizing microtubules and involved in neurite formation.
abstract: We have previously reported that the centriolar protein centlein functions as a molecular link between C-Nap1 and Cep68 to maintain centrosome cohesion [1]. In this study, we identified centlein as a novel microtubule-associated protein (MAP), directly binding to purified microtubules (MTs) via its longest coiled-coil domain. Overexpression of centlein caused profound nocodazole- and cold-resistant MT bundles, which also relied on its MT-binding domain. siRNA-mediated centlein depletion resulted in a significant reduction in tubulin acetylation level and overall fluorescence intensity of cytoplasmic MT acetylation. Centlein was further characterized in neurons. We found that centlein overexpression inhibited neurite formation in retinoic acid (RA)-induced SH-SY5Y and N2a cells. Taken together, we propose that centlein is involved in MT stability and neuritogenesis in vivo.
authors.affiliation: Savaid School of Medicine, University of Chinese Academy of Sciences, Beijing 100049, China; The State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Jing
authors.affiliation: Savaid School of Medicine, University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Huilong
authors.initials: H
authors.lastname: Yin
authors.affiliation: The State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Bioinformatics, Bioinformatics Division and Center for Synthetic & Systems Biology, TNLIST, Department of Automation, Tsinghua University, Beijing 100084, China. Electronic address: jtgao@biomed.tsinghua.edu.cn.
authors.forename: Juntao
authors.initials: J
authors.lastname: Gao
authors.affiliation: Savaid School of Medicine, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: yuanli@ucas.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D049108:Cell Enlargement; D002460:Cell Line; D006801:Humans; D051379:Mice; D008868:Microtubule Proteins / Q000737:chemistry / Q000378:metabolism; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism*; D016501:Neurites / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D055495:Neurogenesis / Q000502:physiology*; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: C579064:CNTLN protein, human; D018797:Cell Cycle Proteins; D008868:Microtubule Proteins
keywords: Acetylated tubulin; Centlein; MAPs; Microtubule bundle; Neurite formation