title: Laser Direct-Write Onto Live Tissues: A Novel Model for Studying Cancer Cell Migration.
abstract: Investigation into the mechanisms driving cancer cell behavior and the subsequent development of novel targeted therapeutics requires comprehensive experimental models that mimic the complexity of the tumor microenvironment. Recently, our laboratories have combined a novel tissue culture model and laser direct-write, a form of bioprinting, to spatially position single or clustered cancer cells onto ex vivo microvascular networks containing blood vessels, lymphatic vessels, and interstitial cell populations. Herein, we highlight this new model as a tool for quantifying cancer cell motility and effects on angiogenesis and lymphangiogenesis in an intact network that matches the complexity of a real tissue. Application of our proposed methodology offers an innovative ex vivo tissue perspective for evaluating the effects of gene expression and targeted molecular therapies on cancer cell migration and invasion. J. Cell. Physiol. 231: 2333-2338, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Medicine, Tulane University, New Orleans, Louisiana.
authors.forename: Hope E
authors.initials: HE
authors.lastname: Burks
authors.affiliation: Department of Biomedical Engineering, Tulane University, New Orleans, Louisiana.
authors.forename: Theresa B
authors.initials: TB
authors.lastname: Phamduy
authors.affiliation: Department of Biomedical Engineering, Tulane University, New Orleans, Louisiana.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Azimi
authors.affiliation: Department of Biomedical Engineering, Tulane University, New Orleans, Louisiana.
authors.forename: Jayant
authors.initials: J
authors.lastname: Saksena
authors.affiliation: Department of Medicine, Tulane University, New Orleans, Louisiana.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Burow
authors.affiliation: Department of Medicine, Tulane University, New Orleans, Louisiana.
authors.forename: Bridgette M
authors.initials: BM
authors.lastname: Collins-Burow
authors.affiliation: Department of Engineering Physics, Tulane University, New Orleans, Louisiana.
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Chrisey
authors.affiliation: Department of Biomedical Engineering, Tulane University, New Orleans, Louisiana.
authors.forename: Walter L
authors.initials: WL
authors.lastname: Murfee
mesh_terms: D000818:Animals; D062028:Bioprinting; D002465:Cell Movement; D006801:Humans; D007834:Lasers; D008954:Models, Biological; D009369:Neoplasms / Q000473:pathology*; D009928:Organ Specificity; D051381:Rats; D059008:Time-Lapse Imaging