title: Effect of Wheat Dietary Fiber Particle Size during Digestion In Vitro on Bile Acid, Faecal Bacteria and Short-Chain Fatty Acid Content.
abstract: The influence of bile acid concentration on the growth of Bifidobacterium spp. and Lactobacillus spp. bacteria was demonstrated. Exposing these bacteria to the environment containing bile acid salts, and very poor in nutrients, leads to the disappearance of these microorganisms due to the toxic effect of bile acids. A multidimensional analysis of data in the form of principal component analysis indicated that lactic acid bacteria bind bile acids and show antagonistic effect on E. coli spp. bacteria. The growth in E. coli spp. population was accompanied by a decline in the population of Bifidobacterium spp. and Lactobacillus spp. with a simultaneous reduction in the concentration of bile acids. This is direct proof of acid binding ability of the tested lactic acid bacteria with respect to cholic acid, lithocholic acid and deoxycholic acid. This research demonstrated that the degree of fineness of wheat dietary fibre does not affect the sorption of bile acids and growth of some bacteria species; however, it has an impact on the profile of synthesized short-chained fatty acids. During the digestion of a very fine wheat fibre fraction (WF 90), an increase in the concentration of propionic and butyric acids, as compared with the wheat fiber fraction of larger particles - WF 500, was observed. Our study suggested that wheat fibre did not affect faecal bacteria growth, however, we observed binding of bile acids by Bifidobacterium spp. and Lactobacillus spp.
authors.affiliation: Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572, Poznań, Poland. dziedzic@ump.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Dziedzic
authors.affiliation: Institute of Food Technology and Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624, Poznań, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Szwengiel
authors.affiliation: Department of Food Service and Catering, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624, Poznań, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Górecka
authors.affiliation: Department of Commodity Sciences and Food Analysis, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-957, Olsztyn, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Gujska
authors.affiliation: Department of Food Service and Catering, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624, Poznań, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kaczkowska
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627, Poznań, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Drożdżyńska
authors.affiliation: Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572, Poznań, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Walkowiak
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development; D001647:Bile Acids and Salts / Q000378:metabolism*; D019826:Cholic Acid / Q000378:metabolism; D004043:Dietary Fiber / Q000494:pharmacology*; D004063:Digestion / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008095:Lithocholic Acid / Q000378:metabolism; D010316:Particle Size; D014908:Triticum / Q000737:chemistry*
chemical_list: D001647:Bile Acids and Salts; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D008095:Lithocholic Acid; D019826:Cholic Acid
keywords: Bile acids; Faecal bacteria; In vitro digestion; Short-chain fatty acids; Wheat fibre