title: The filamentous fungus Ashbya gossypii as a competitive industrial inosine producer.
abstract: Inosine is a nucleoside with growing biotechnological interest due to its recently attributed beneficial health effects and as a convenient precursor of the umami flavor. At present, most of the industrial inosine production relies on bacterial fermentations. In this work, we have metabolically engineered the filamentous fungus Ashbya gossypii to obtain strains able to excrete high amounts of inosine to the culture medium. We report that the disruption of only two key genes of the purine biosynthetic pathway efficiently redirect the metabolic flux, increasing 200-fold the excretion of inosine with respect to the wild type, up to 2.2 g/L. These results allow us to propose A. gossypii as a convenient candidate for large-scale nucleoside production, especially in view of the several advantages that Ashbya has with respect to the bacterial systems used at present for the industrial production of this food additive. Biotechnol. Bioeng. 2016;113: 2060-2063. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Metabolic Engineering Group, Departamento de Microbiologia y Genetica, Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, 37007, Spain.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Ledesma-Amaro
authors.affiliation: Metabolic Engineering Group, Departamento de Microbiologia y Genetica, Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, 37007, Spain.
authors.forename: Rubén M
authors.initials: RM
authors.lastname: Buey
authors.affiliation: Metabolic Engineering Group, Departamento de Microbiologia y Genetica, Universidad de Salamanca, Campus Miguel de Unamuno, Salamanca, 37007, Spain. revuelta@usal.es.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Revuelta
mesh_terms: D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D055169:Eremothecium / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D007288:Inosine / Q000032:analysis / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D003470:Culture Media; D007288:Inosine
keywords: Ashbya gossypii; fermentation; inosine; metabolic engineering