title: Phenolic contents and bioactive potential of peach fruit extracts.
abstract: Several cultivars of peach fruit (Prunus persica L.) were investigated. Their phenolic composition and concentration were assessed by LC-MS. Concentrations were calculated in mg per g of dry weight extract. Their antioxidant capacity (Folin-Ciocalteu, ORAC, DPPH, ABTS, PFRAP and ICA), inhibitory property against β-amyloid and α-synuclein fibril formation and protective capacity against Aβ-induced toxicity on PC12 cell lines (viability assessed by MTT assay and intracellular ROS production by DCFH-DA assay) were evaluated. Fifteen different phenolic compounds were identified and quantified. In particular, new isorhamnetin derivatives were identified. Phenolic contents were ranged between 19 and 82mg/g. Spring Belle extract had the highest content and Romea the lowest. Except for the ICA assay, a good correlation between phenolic content and the antioxidant capacities of peach fruit extracts was found, indicating that phenolic compounds are major contributors to their antioxidant capacity. Results indicate that the phenolic extract of peach cultivars inhibits Aβ and αS fibril formation and protects PC12 cell lines against Aβ-induced toxicity.
authors.affiliation: Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria; Université de Poitiers, Laboratoire STIM, ERL7368-CNRS, Equipe IP2C, Pôle Biologie Santé, Bat. B36, 1 rue Georges Bonnet, 86073 Poitiers Cedex 09, France.
authors.forename: Abderrahmane
authors.initials: A
authors.lastname: Mokrani
authors.affiliation: Université de Bordeaux, ISVV, EA 3675, GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.affiliation: Université de Bordeaux, ISVV, EA 3675, GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Cluzet
authors.affiliation: Université de Bordeaux, ISVV, EA 3675, GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Da Costa
authors.affiliation: Université de Bordeaux, ISVV, EA 3675, GESVAB, 33140 Villenave d'Ornon, France.
authors.forename: Hamza
authors.initials: H
authors.lastname: Temsamani
authors.affiliation: Université de Bordeaux, ISVV, 33140 Villenave d'Ornon, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Renouf
authors.affiliation: Université de Bordeaux, ISVV, EA 3675, GESVAB, 33140 Villenave d'Ornon, France; Université de Bordeaux, ISVV, 33140 Villenave d'Ornon, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.affiliation: Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Khodir
authors.initials: K
authors.lastname: Madani
authors.affiliation: Université de Poitiers, Laboratoire STIM, ERL7368-CNRS, Equipe IP2C, Pôle Biologie Santé, Bat. B36, 1 rue Georges Bonnet, 86073 Poitiers Cedex 09, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Mesnil
authors.affiliation: Université de Poitiers, Laboratoire STIM, ERL7368-CNRS, Equipe IP2C, Pôle Biologie Santé, Bat. B36, 1 rue Georges Bonnet, 86073 Poitiers Cedex 09, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Monvoisin
authors.affiliation: Université de Bordeaux, ISVV, EA 3675, GESVAB, 33140 Villenave d'Ornon, France. Electronic address: tristan.richard@u-bordeaux.fr.
authors.forename: Tristan
authors.initials: T
authors.lastname: Richard
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D002853:Chromatography, Liquid; D005452:Fluoresceins; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry; D016716:PC12 Cells; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D000068239:Prunus persica; D051381:Rats; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005452:Fluoresceins; D010636:Phenols; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein
keywords: Antioxidant activity; Polyphenol; Prunus persica L.; α-synuclein; β-amyloid