title: Correlation of Helicobacter pylori and interleukin-8 mRNA expression in high risk gastric cancer population prediction.
abstract: OBJECTIVE
  To evaluate (1) the association of the Helicobacter pylori (H. pylori) test and interleukin-8 (IL-8) mRNA expression alone and the severity of gastric cancer (GC); (2) the association of both tests were added to patients' characteristics to identifli Thai suspected patients of gastric cancer who would receive the most benefit; and (3) diagnostic value of levels of IL-8 mRNA expression for gastric cancer.
  
  
  METHODS
  A cross-sectional analytical study was completed with 220 patients with 86 GC patients who underwent endoscopy with gastric surgery divided into non-metastasis and metastasis groups, and 134 patients with benign lesions who underwent endoscopic examination, at the Gastrointestinal Surgery and Endoscopy Unit, Chiang Mai University Hospital between 2006 and 2010. Of 220 patients, 86 cases of diagnosed gastric adenocarcinoma were in an advanced stage and 134 cases were non-cancer patients.
  
  
  RESULTS
  The IL-8 mRNA expression showed predominant association with advanced GC when compared to H. pylori infection alone [OR (95%CI); 0.86 (0.49-1.53) vs 5.44 (3.08-9.62)] when including the patients' characteristics the highest of the area under the receiver operating characteristic curves (AuROC) of the model were males older than 40 years of age [AuROC (95%CI); 0.81 (0.75-0.86)]. However, preliminary testing for diagnostic indices of four cut-off points of IL-8 mRNA expression to predict the severity of GC cases found an increasing suboptimal trend from the likelihood ratio of positive to differentiate the severity in the GC group. The IL-8 mRNA expression showed a predominant association with GC when compared to H. pylori infection, especially in males older than 40 years of age who may benefit most from this test.
  
  
  CONCLUSIONS
  The future research of IL-8 mRNA expression to predict severity in the gastric cancer group should be warranted.
authors.affiliation: Wilaiwan Chongruksut, Sirikan Limpakan (Yamada), Bandhuphat Chakrabandhu, Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Wilaiwan
authors.initials: W
authors.lastname: Chongruksut
authors.affiliation: Wilaiwan Chongruksut, Sirikan Limpakan (Yamada), Bandhuphat Chakrabandhu, Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Sirikan
authors.initials: S
authors.lastname: Limpakan Yamada
authors.affiliation: Wilaiwan Chongruksut, Sirikan Limpakan (Yamada), Bandhuphat Chakrabandhu, Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Bandhuphat
authors.initials: B
authors.lastname: Chakrabandhu
authors.affiliation: Wilaiwan Chongruksut, Sirikan Limpakan (Yamada), Bandhuphat Chakrabandhu, Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Chidchanok
authors.initials: C
authors.lastname: Ruengorn
authors.affiliation: Wilaiwan Chongruksut, Sirikan Limpakan (Yamada), Bandhuphat Chakrabandhu, Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Sirisak
authors.initials: S
authors.lastname: Nanta
keywords: Gastric cancer; Helicobacter pylori; Interleukin-8 mRNA expression