title: [Expression and clinical significance of 5hmC in bladder urothelial carcinoma].
abstract: OBJECTIVE
  To investigate the expression of 5-hydroxymethylcytosine (5hmC) in bladder urothelial carcinoma (UC) and its clinical significance.
  
  
  METHODS
  The expression of 5hmC in 21 cases of UC tissues and pericarcinous urinary tract epithelium was detected by immunohistochemical staining. Then the expression of 5hmC in the surgical resection of UC tissues in 92 cases was also surveyed. Non parametric U Mann-Whitney test was used to analyze the correlation between 5hmC expression and clinical data. Single factor survival analysis was performed by Kaplan-Meier test.
  
  
  RESULTS
  The expression of 5hmC in normal urinary tract epithelium and UC tissues was significantly different, but there was no significant difference in the expression of 5hmC between low and high grades of UC tissues as well as between different TNM grades. Kaplan-Meier single factor survival analysis showed that there was no significant correlation between the 5hmC expression level and the survival rate or the recurrence-free survival of UC patients.
  
  
  CONCLUSIONS
  The expression level of 5hmC in UC tissues is significantly lower than that in pericarcinous urinary tract epithelium. There is no correlation between the 5hmC expression and the progression, prognosis and recurrence of UC.
authors.affiliation: Department of Pathology, Basic Medical College, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Basic Medical College, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yuqiao
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pathology, Basic Medical College, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Company 13, Student Brigade, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Company 13, Student Brigade, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhekai
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Basic Medical College, Fourth Military Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: zhf1975@fmmu.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Basic Medical College, Fourth Military Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: liqing@fmmu.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000473:pathology; D003596:Cytosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine