title: Induction of Mitochondria Mediated Apoptosis in Human Breast Cancer Cells (T-47D) by Annona reticulata L. Leaves Methanolic Extracts.
abstract: Annona reticulata Linn. (Common name: Bullock's-heart) (Annonaceae family) is a semi-evergreen and small deciduous tree. The extracts of various parts of Annona reticulata L. have been reported as cytotoxic to many cancer cells. Annona reticulata L. leaves' methanolic extract (ARME) was prepared and used against the breast cancer cells. The breast cancer cells (T-47D) viability and IC50 were evaluated by Vybrant® MTT Cell Proliferation Assay Kit. Detection of phosphatidylserine on membranes of apoptotic cells was done by Attune flow cytometer. RNA transcripts were quantified in ARME treated and untreated cells. Finally, the Vybrant® FAM Poly Caspases assay kit was used for analysis of polycaspases activity in T-47D cells. The IC50 (5 ± 0.5 µg/mL) of the ARME was found against breast cancer cells (T-47D). The Paclitaxel was used as a control standard drug for the study. The downregulation of Bcl-2 and upregulation of Bax and Bak, and caspases activation suggested induction of apoptosis in T-47D cells by ARME through mitochondrial pathway. The cell cycle halted at G2/M phase in the ARME treated cells. The ARME was found to be effective against Breast cancer cells (T-47D).
authors.affiliation: a Centre for Advanced Life Sciences, Deogiri College , Aurangabad , India.
authors.forename: Pratiksha H
authors.initials: PH
authors.lastname: Roham
authors.affiliation: a Centre for Advanced Life Sciences, Deogiri College , Aurangabad , India.
authors.forename: Kiran R
authors.initials: KR
authors.lastname: Kharat
authors.affiliation: a Centre for Advanced Life Sciences, Deogiri College , Aurangabad , India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Mungde
authors.affiliation: a Centre for Advanced Life Sciences, Deogiri College , Aurangabad , India.
authors.forename: Mahadev A
authors.initials: MA
authors.lastname: Jadhav
authors.affiliation: a Centre for Advanced Life Sciences, Deogiri College , Aurangabad , India.
authors.forename: Surinder J
authors.initials: SJ
authors.lastname: Makhija
mesh_terms: D031041:Annona / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D020169:Caspases / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C494772:BAK1 protein, human; D010936:Plant Extracts; D050998:bcl-2 Homologous Antagonist-Killer Protein; D020169:Caspases; D000432:Methanol