title: 3D visualization of the lumbar facet joint after degeneration using propagation phase contrast micro-tomography.
abstract: Lumbar facet joint (LFJ) degeneration is believed to be an important cause of low back pain (LBP). Identifying the morphological changes of the LFJ in the degeneration process at a high-resolution level could be meaningful for our better understanding of the possible mechanisms underlying this process. In the present study, we determined the 3D morphology of the LFJ using propagation phase contrast micro-tomography (PPCT) in rats to assess the subtle changes that occur during the degeneration process. PPCT provides vivid 3D images of micromorphological changes in the LFJ during its degeneration process, and the changes in the subchondral bone occurred earlier than in the cartilage during the early stage of degeneration of the LFJ. The delineation of this alteration was similar to that with the histological method. Our findings demonstrated that PPCT could serve as a valuable tool for 3D visualization of the morphology of the LFJ by providing comprehensive information about the cartilage and the underlying subchondral bone and their changes during degeneration processes. It might also have great potential for providing effective diagnostic tools to track changes in the cartilage and to evaluate the effects of therapeutic interventions for LFJ degeneration in preclinical studies.
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Drug Delivery System, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Sports Medicine, Research Centre of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Chunyue
authors.initials: C
authors.lastname: Duan
mesh_terms: D000818:Animals; D002356:Cartilage / Q000000981:diagnostic imaging* / Q000473:pathology; D021621:Imaging, Three-Dimensional; D055959:Intervertebral Disc Degeneration / Q000473:pathology; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D055114:X-Ray Microtomography; D021801:Zygapophyseal Joint / Q000000981:diagnostic imaging* / Q000473:pathology