title: Molecular analysis of the immunoglobulin genes in goose.
abstract: Immunoglobulins play an important role in adaptive immune system as defense molecules against pathogens. However, our knowledge on avian immunoglobulin genes has been limited to a few species. In this study, we analyzed goose (Anser cygnoides orientalis) immunoglobulin genes. Three IgH classes including IgM, IgA, IgY and λ light chain were identified. The IgM and IgA heavy chain constant regions are characteristically similar to their counterparts described in other vertebrates. In addition to the classic Ig isotypes, we also detected a transcript that encoded a truncated form of IgY (IgY(ΔFc)) in goose. Similar to duck, the IgY(ΔFc) in goose was generated by using different transcriptional termination signal of the same υ gene. Limited variability and only one leader peptide were observed in VH and VL domains, which suggested that gene conversion was the primary mechanism involved in goose antibody diversity. Our study provides more insights into the immunoglobulin genes in goose that had not been fully explored before.
authors.affiliation: School of Life Science, Henan University, Kaifeng 475004, PR China; Institute of Bioengineering, Henan University, Kaifeng 475004, PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Huang
authors.affiliation: Institute of Bioengineering, Henan University, Kaifeng 475004, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.affiliation: School of Life Science, Henan University, Kaifeng 475004, PR China; Institute of Bioengineering, Henan University, Kaifeng 475004, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Yuan
authors.affiliation: School of Life Science, Henan University, Kaifeng 475004, PR China; Institute of Bioengineering, Henan University, Kaifeng 475004, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shao
authors.affiliation: School of Life Science, Henan University, Kaifeng 475004, PR China; Institute of Bioengineering, Henan University, Kaifeng 475004, PR China.
authors.forename: WenYuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Life Science, Henan University, Kaifeng 475004, PR China; Institute of Bioengineering, Henan University, Kaifeng 475004, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Wei
authors.affiliation: School of Life Science, Henan University, Kaifeng 475004, PR China; Institute of Bioengineering, Henan University, Kaifeng 475004, PR China. Electronic address: gscao@henu.edu.cn.
authors.forename: Gengsheng
authors.initials: G
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005777:Geese / Q000235:genetics*; D005803:Genes, Immunoglobulin; D007132:Immunoglobulin Isotypes / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D010802:Phylogeny
chemical_list: D030161:Avian Proteins; D007132:Immunoglobulin Isotypes; D007135:Immunoglobulin Variable Region
keywords: Evolution; Goose; Immunoglobulin gene