title: LncRNA PANDAR regulates the G1/S transition of breast cancer cells by suppressing p16(INK4A) expression.
abstract: It has been reported that lncRNA PANDAR (promoter of CDKN1A antisense DNA damage-activated RNA) is induced as a result of DNA damage, and it regulates the reparation of DNA damage. In this study, we investigated the role of lncRNA PANDAR in the progression of breast cancer and found that PANDAR was up-regulated in breast cancer tissues and cell lines. The knockdown of PANDAR suppresses G1/S transition of breast cancer cells. We demonstrated mechanistically that the regulation of G1/S transition by PANDAR was partly due to the transcriptional modulation of p16(INK4A). Moreover, we showed that PANDAR impacted p16(INK4A) expression by regulating the recruitment Bmi1 to p16(INK4A) promoter. To our knowledge, this is the first study which showed the functional roles and mechanisms of PANDAR in regulating the progression of breast cancer. The PANDAR/Bmi1/p16(INK4A) axis could serve as novel targets for breast cancer therapy.
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sang
authors.affiliation: Department of Gastroenterology, Cancer Hospital of Jiangxi Province, Nanchang, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Radiation Oncology, the Affiliated Hospital of Guilin Medical University, P.R. China.
authors.forename: Siwei
authors.initials: S
authors.lastname: Li
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, P.R. China.
authors.forename: XiaoFeng
authors.initials: X
authors.lastname: Tang
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, P.R. China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Qian
authors.affiliation: Department of Medicine, GaomingHeshui Hospital, Foshan, P.R. China.
authors.forename: Liang-Ming
authors.initials: LM
authors.lastname: Deng
authors.affiliation: Department of pharmacy, Cancer Hospital of Jiangxi Province, Nanchang, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, the Third Affiliated Hospital, Nanchang University, Nanchang, P.R. China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Lv
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D061986:MCF-7 Cells; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C069077:BMI1 protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004268:DNA-Binding Proteins; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C000612163:long non-coding RNA PANDAR, human; D063150:Polycomb Repressive Complex 1