title: Effects of peripherally administered cholecystokinin-8 and secretin on feeding/drinking and oxytocin-mRFP1 fluorescence in transgenic rats.
abstract: Peripheral administration of cholecystokinin (CCK)-8 or secretin activates oxytocin (OXT)-secreting neurons in the hypothalamus. Although OXT is involved in the regulation of feeding behavior, detailed mechanism remains unclear. In the present study, we examined the central OXTergic pathways after intraperitoneally (i.p.) administration of CCK-8 and secretin using male OXT-monomeric red fluorescent protein 1 (mRFP1) transgenic rats and male Wistar rats. I.p. administration of CCK-8 (50μg/kg) and secretin (100μg/kg) decreased food intake in these rats. While i.p. administration of CCK-8 decreased water intake, i.p. administration of secretin increased water intake. Immunohistochemical study revealed that Fos-Like-Immunoreactive cells were observed abundantly in the brainstem and in the OXT neurons in the dorsal division of the parvocellular paraventricular nucleus (dpPVN). We could observe marked increase of mRFP1 fluorescence, as an indicator for OXT, in the dpPVN and mRFP1-positive granules in axon terminals of the dpPVN OXT neurons in the nucleus tractus solitarius (NTS) after i.p. administration of CCK-8 and secretin. These results provide us the evidence that, at least in part, i.p. administration of CCK-8 or secretin might be involved in the regulation of feeding/drinking via a OXTergic pathway from the dpPVN to the NTS.
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Motojima
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawasaki
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan; Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Matsuura
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Saito
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yoshimura
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hashimoto
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ueno
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Department of Orthopaedics, Wakamatsu Hospital for University of Occupational and Environmental Health, Kitakyushu 808-0024, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Ohnishi
authors.affiliation: Department of Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Sakai
authors.affiliation: Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan. Electronic address: yoichi@med.uoeh-u.ac.jp.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Ueta
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000378:metabolism; D002766:Cholecystokinin; D004327:Drinking Behavior / Q000187:drug effects*; D005247:Feeding Behavior / Q000187:drug effects*; D005453:Fluorescence; D007031:Hypothalamus / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008164:Luminescent Proteins / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D010121:Oxytocin / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism; D055647:Rats, Transgenic; D017208:Rats, Wistar; D012633:Secretin / Q000494:pharmacology*; D012844:Sincalide / Q000494:pharmacology*; D000097573:Red Fluorescent Protein
chemical_list: D002766:Cholecystokinin; D008164:Luminescent Proteins; D010121:Oxytocin; D012633:Secretin; D012844:Sincalide; D000097573:Red Fluorescent Protein
keywords: Brainstem; Cholecystokinin-8; Fluorescent protein; Hypothalamus; Oxytocin; Secretin