title: Super-Resolved Traction Force Microscopy (STFM).
abstract: Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. This improvement is highlighted by computer simulations and an activating RBL cell model system.
authors.forename: Huw
authors.initials: H
authors.lastname: Colin-York
authors.forename: Dilip
authors.initials: D
authors.lastname: Shrestha
authors.forename: James H
authors.initials: JH
authors.lastname: Felce
authors.forename: Dominic
authors.initials: D
authors.lastname: Waithe
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02142, United States.|Department of Mechanical Engineering, University College London , WC1E 7JE London, United Kingdom.
authors.forename: Emad
authors.initials: E
authors.lastname: Moeendarbary
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Davis
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Marco
authors.initials: M
authors.lastname: Fritzsche
mesh_terms: D000465:Algorithms; D002448:Cell Adhesion; D003198:Computer Simulation; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D020527:Microscopy, Scanning Probe / Q000295:instrumentation / Q000379:methods; D008962:Models, Theoretical; D013314:Stress, Mechanical; D014143:Traction
keywords: Super-resolution microscopy; actin cytoskeleton; mechanobiology; traction force microscopy