title: The effect of spraying different disinfectants on condensational silicone impressions; an in vitro study.
abstract: BACKGROUND
  Dentistry equipment are exposed to different types of pathogenic microorganisms. The aim of this study was to investigate the effect of spraying three different types of disinfectants on condensational silicones after 5 and 10 min.
  
  
  METHODS
  Totally, 66 circular samples of condensational silicone impression materials of 1 cm diameter and 2 mm thickness were contaminated by Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans fungus. Except for control samples, all of them were disinfected with sodium hypochlorite (NaOCl) 0.525%, Deconex and Epimax by spraying method. Afterward, they kept in plastic bags with humid rolled cotton for 5 and 10 min. In order to isolate microbiotas, the samples were immersed in 2% trypsin for 1 h and diluted with normal saline in a portion of 1, 1/2, and 1/4. The trypsin suspensions were transferred to culture plates for incubation and colony-forming unit assay. The data were analyzed by Mann-Whitney test and SPSS software version 16 at a significant level of 0.05.
  
  
  RESULTS
  There was a meaningful difference between disinfection effects of Epimax-Deconex for all mentioned microorganisms after 5 min (P = 0.034), and between disinfection effects of NaOCl 0.525%-Epimax for S. aureus (P = 0.043) and P. aeruginosa (P = 0.046) after 5 min. Furthermore, there was a meaningful difference between disinfection effects of Epimax-Deconex (P = 0.034) and NaOCl 0.525%-Epimax (P = 0.034) for P. aeruginosa after 10 min.
  
  
  CONCLUSIONS
  Condensational silicone can be effectively disinfected by spraying tested three disinfecting agents. More specifically, Deconex showed the best results compared to the other agents.
authors.affiliation: Department of Operative, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Badrian
authors.affiliation: Dental Students Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Davoudi
authors.affiliation: Department of Prosthodontics, School of Dentistry, Shahid Sadoughi University Medical Sciences, Yazd, Iran.
authors.forename: Meysam
authors.initials: M
authors.lastname: Molazem
authors.affiliation: Department of Prosthodontics, School of Dentistry, Shahid Sadoughi University Medical Sciences, Yazd, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Zare
keywords: Condensational silicone; disinfection; impression materials; spray