title: Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice.
abstract: Self-assembled DNA nanostructures, as a new type of nanocarriers, have shown great potential in molecular imaging probes. DNA bipyramid nanostructures (DBNs), as a classic kind of DNA nanostructure, can be precisely constructed through the unparalleled base-pairing precision of oligonucleotide strands. DBNs were prepared by self-assembly of six oligonucleotides with equal molar amounts in a single annealing step, and purified by high performance liquid chromatography (HPLC). DBNs were stable in 10% FBS as well as 80% mouse serum for at least 8 h. To prepare (99m)Tc-labeled DBNs, N-hydroxysuccinimidyl S-acetylmercaptoacetyl triglycinate (Sacetyl-MAG3-NHS ester) tagged single-stranded DNA (ssDNA) was first radiolabeled with Tc-99m, and DBNs with an overhang were assembled and then hybridized with (99m)Tc-ssDNA to prepare (99m)Tc-labeled DNA bipyramid nanostructures ((99m)Tc-MAG3-DBNs). DBNs were radiolabeled, with the radiochemical purity being over 90%. The plasma half-life of (99m)Tc-MAG3-DBNs in normal KM mice was about 6 min. The biodistribution and SPECT/CT imaging were conducted with (99m)Tc-MAG3-DBNs in KM mice, and both showed that (99m)Tc-MAG3-DBNs mainly concentrated in the intestine, liver, and kidneys, and there was also prominent uptake in the gallbladder and bladder. We successfully obtained a new class of SPECT molecular probes based on this DNA polyhedron structure.
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital , No. 1 Tongdao North Street, Hohhot 010050, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Li
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences , 2019 Jia Luo Road, Shanghai 201800, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital , No. 1 Tongdao North Street, Hohhot 010050, China.
authors.forename: Baoliang
authors.initials: B
authors.lastname: Bao
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital , No. 1 Tongdao North Street, Hohhot 010050, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital , No. 1 Tongdao North Street, Hohhot 010050, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital , No. 1 Tongdao North Street, Hohhot 010050, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D051379:Mice; D015335:Molecular Probes; D015609:Organotechnetium Compounds / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D015609:Organotechnetium Compounds; D004247:DNA