title: Cell-based Assays for Assessing Toxicity: A Basic Guide.
abstract: Assessment of toxicity is an important component of the drug discovery process. Cellbased assays are a popular choice for assessing cytotoxicity. However, these assays are complex because of the wide variety of formats and methods that are available, lack of standardization, confusing terminology and the inherent variability of biological systems and measurement. This review is intended as a guide on how to take these factors into account when planning, conducting and/or interpreting cell based toxicity assays.
authors.affiliation: Department of Virology, National Health Laboratory Service/University of KwaZulu-Natal, Durban, South Africa.. South Africa.
authors.forename: Raveen
authors.initials: R
authors.lastname: Parboosing
authors.forename: Gugulethu
authors.initials: G
authors.lastname: Mzobe
authors.forename: Louis
authors.initials: L
authors.lastname: Chonco
authors.forename: Indres
authors.initials: I
authors.lastname: Moodley
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001681:Biological Assay; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015394:Molecular Structure; D013329:Structure-Activity Relationship
keywords: Cytotoxicity; apoptosis; cell-based assays; cytotoxicity screening; toxicity.; viability assays