title: Visualizing the molecular sociology at the HeLa cell nuclear periphery.
abstract: The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure-the nuclear pore complex-revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness.
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany. mahamid@biochem.mpg.de baumeis@biochem.mpg.de.
authors.forename: Julia
authors.initials: J
authors.lastname: Mahamid
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Pfeffer
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Schaffer
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany. Department of Chemistry and Biochemistry, University of California, San Diego, CA, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Villa
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Radostin
authors.initials: R
authors.lastname: Danev
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Luis Kuhn
authors.initials: LK
authors.lastname: Cuellar
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Förster
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Jürgen M
authors.initials: JM
authors.lastname: Plitzko
authors.affiliation: Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, 82152 Martinsried, Germany. mahamid@biochem.mpg.de baumeis@biochem.mpg.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumeister
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D055032:Electron Microscope Tomography / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D034881:Nuclear Lamina / Q000648:ultrastructure*; D022022:Nuclear Pore / Q000648:ultrastructure*; D009707:Nucleosomes / Q000648:ultrastructure; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000145:classification / Q000648:ultrastructure
chemical_list: D009707:Nucleosomes