title: Dengue virus non-structural 1 protein interacts with heterogeneous nuclear ribonucleoprotein H in human monocytic cells.
abstract: OBJECTIVE
  To study protein-protein interaction between heterogeneous nuclear ribonucleoprotein H (hnRNP H) and Dengue virus (DENV) proteins.
  
  
  METHODS
  DENV proteins were screened against the host hnRNP H protein, in order to identify the host-viral protein-protein interactions in DENV infected THP-1 cells by co-immunoprecipitation. The co-localization of the interacting proteins was further confirmed by immunofluorescence microscopy.
  
  
  RESULTS
  The host protein hnRNP H was found to interact with DENV non-structural 1 protein and help the virus to multiply in the cell.
  
  
  CONCLUSIONS
  The non-structural 1 glycoprotein is a key modulator of host immune response and is also involved in viral replication. Therefore, disruption of this key interaction between hnRNP H and DENV non-structural 1 could be an important therapeutic strategy for management of DENV infection.
authors.affiliation: Immunomodulation Lab, Defence Institute of Physiology & Allied Sciences, Delhi, India.
authors.forename: Drishya
authors.initials: D
authors.lastname: Diwaker
authors.affiliation: Immunomodulation Lab, Defence Institute of Physiology & Allied Sciences, Delhi, India. Electronic address: kpmpgi@rediffmail.com.
authors.forename: K P
authors.initials: KP
authors.lastname: Mishra
authors.affiliation: Immunomodulation Lab, Defence Institute of Physiology & Allied Sciences, Delhi, India.
authors.forename: L
authors.initials: L
authors.lastname: Ganju
authors.affiliation: Immunomodulation Lab, Defence Institute of Physiology & Allied Sciences, Delhi, India.
authors.forename: S B
authors.initials: SB
authors.lastname: Singh
keywords: Dengue virus; Heterogeneous nuclear ribonucleoprotein H; Non-structural 1 protein; Protein interactions