title: Crystallographic studies of the complex of human HINT1 protein with a non-hydrolyzable analog of Ap4A.
abstract: Histidine triad nucleotide-binding protein 1 (HINT1) represents the most ancient and widespread branch in the histidine triad proteins superfamily. HINT1 plays an important role in various biological processes, and it has been found in many species. Here, we report the first structure (at a 2.34Å resolution) of a complex of human HINT1 with a non-hydrolyzable analog of an Ap4A dinucleotide, containing bis-phosphorothioated glycerol mimicking a polyphosphate chain, obtained from a primitive monoclinic space group P21 crystal. In addition, the apo form of hHINT1 at the space group P21 refined to 1.92Å is reported for comparative studies.
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łódź, Poland. Electronic address: rdolot@cbmm.lodz.pl.
authors.forename: Rafał
authors.initials: R
authors.lastname: Dolot
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łódź, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Kaczmarek
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łódź, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Sęda
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łódź, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Krakowiak
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łódź, Poland.
authors.forename: Janina
authors.initials: J
authors.lastname: Baraniak
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łódź, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Nawrot
mesh_terms: D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D015226:Dinucleoside Phosphates / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary
chemical_list: D001059:Apoproteins; D015226:Dinucleoside Phosphates; C063306:HINT1 protein, human; D009419:Nerve Tissue Proteins; C020733:diadenosine tetraphosphate
keywords: Ap4A analog; Histidine triad nucleotide-binding protein; Protein crystallography