title: Comparative Proteomic Analysis of Cultured Suspension Cells of the Halophyte Halogeton glomeratus by iTRAQ Provides Insights into Response Mechanisms to Salt Stress.
abstract: Soil salinity severely threatens land use capability and crop yields worldwide. An analysis of the molecular mechanisms of salt tolerance in halophytes will contribute to the development of salt-tolerant crops. In this study, a combination of physiological characteristics and iTRAQ-based proteomic approaches was conducted to investigate the molecular mechanisms underlying the salt response of suspension cell cultures of halophytic Halogeton glomeratus. These cells showed halophytic growth responses comparable to those of the whole plant. In total, 97 up-regulated proteins and 192 down-regulated proteins were identified as common to both 200 and 400 mM NaCl concentration treatments. Such salinity responsive proteins were mainly involved in energy, carbohydrate metabolism, stress defense, protein metabolism, signal transduction, cell growth, and cytoskeleton metabolism. Effective regulatory protein expression related to energy, stress defense, and carbohydrate metabolism play important roles in the salt-tolerance of H. glomeratus suspension cell cultures. However, known proteins regulating Na(+) efflux from the cytoplasm and its compartmentalization into the vacuole did not change significantly under salinity stress suggesting our existing knowledge concerning Na(+) extrusion and compartmentalization in halophytes needs to be evaluated further. Such data are discussed in the context of our current understandings of the mechanisms involved in the salinity response of the halophyte, H. glomeratus.
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Juncheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Yao
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Botany, College of Life Science and Technology, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Baochun
authors.initials: B
authors.lastname: Li
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Yaxiong
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Agriculture and Forestry, College of Agriculture and Animal Husbandry, Qinghai University Xining, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lai
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Erjing
authors.initials: E
authors.lastname: Si
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Panrong
authors.initials: P
authors.lastname: Ren
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural University Lanzhou, China.
authors.forename: Xunwu
authors.initials: X
authors.lastname: Shang
authors.affiliation: Gansu Provincial Key Lab of Aridland Crop Science/Gansu Key Lab of Crop Improvement and Germplasm EnhancementLanzhou, China; Department of Crop Genetics and Breeding, College of Agronomy, Gansu Agricultural UniversityLanzhou, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Wang
keywords: H. glomeratus; cells; halophyte; iTRAQ; response mechanisms; salt tolerance