title: Detection of human papillomavirus infection by molecular tests and its relation to colonic polyps and colorectal cancer.
abstract: OBJECTIVE
  To prospectively examine the association between human papilloma virus (HPV) colonization of the colonic mucosa and the development of colorectal polyps (CRPs), and colorectal cancer (CRC) in Saudi Arabia.
  
  
  METHODS
  A case control study was performed between January 2013 and December 2014. All eligible patients underwent standard diagnostic colonoscopy. Patients with polyps or colorectal cancer were considered cases, while those with any other endoscopic findings were controls. Biopsy samples from polyps and tumors, and/or from normal colonic mucosa were acquired. Human papilloma virus colonization was detected using a hybrid capture technique of samples taken from both normal tissue, and CRPs and CRC. The association between HPV and CRPs/CRC was evaluated.
  
  
  RESULTS
  A total of 132 patients were recruited. The mean age was 53 (± 15.9) years. Sixty patients had endoscopically detectable CRPs/CRC, and 72 had either inflammation or normal endoscopic evaluations. Only 4 (0.8%) of the 132 samples that were collected and analyzed were positive for the HPV gene. Statistical analysis did not identify any significant association between HPV colonization and the presence of CRPs/CRC. The only significant predictor of detecting CRPs/CRC on colonoscopy was symptomatic presentation (odds ratio=11.072, 95% confidence interval 4.7-26.2, p less than 0.001).
  
  
  CONCLUSIONS
  Human papilloma virus colonic colonization is rare in Saudi Arabia. An association between HPV colonization and CRP/CRC development could not be identified in this cohort of patients.
authors.affiliation: Department of Microbiology, King Abdulaziz University Hospital, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. E-mail. fatengazzaz@hotmail.com.
authors.forename: Faten
authors.initials: F
authors.lastname: Gazzaz
authors.forename: Mahmoud H
authors.initials: MH
authors.lastname: Mosli
authors.forename: Hani
authors.initials: H
authors.lastname: Jawa
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Sibiany
mesh_terms: D000236:Adenoma / Q000453:epidemiology* / Q000821:virology; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000453:epidemiology* / Q000821:virology; D016022:Case-Control Studies; D003111:Colonic Polyps / Q000453:epidemiology* / Q000821:virology; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology*; D011446:Prospective Studies; D012529:Saudi Arabia / Q000453:epidemiology
chemical_list: D004279:DNA, Viral