title: Impact of JAK2V617F Mutation Burden on Disease Phenotype in Chinese Patients with JAK2V617F-positive Polycythemia Vera (PV) and Essential thrombocythemia (ET).
abstract: Most patients with polycythemia vera (PV) and half of essential thrombocythemia (ET) possess an activating JAK2V617F mutation. The objective of this study was to better define the effect of JAK2V617F mutant allele burden on clinical phenotypes in Chinese patients, especially thrombosis. By real-time polymerase chain reaction (RT-PCR), the JAK2V617F mutation burden was detected in 170 JAK2V617F-positive patients, including 54 PV and 116 ET. The results showed that JAK2V617F allele burden was higher in PV than in ET (P< 0.001). Higher percentage of patients had JAK2V617F allele burden over 20% in PV than in ET (68.5% VS 26.7%) (P< 0.001). In PV patients, higher JAK2V617F allele burden was observed in female (P< 0.05) and leukocytosis patients (WBC above 10 × 10(9)/L) (P< 0.001). Meanwhile, ET patients showed increased JAK2V617F allele burden in the group with higher hemoglobin (HGB above 150 g/L) (P< 0.05), leukocytosis (WBC above 10 × 10(9)/L) (P< 0.001), splenomegaly (P< 0.05) and thrombosis (P< 0.05). In conclusion, the JAK2V617F mutation allele burden is higher in Chinese patients with PV than ET. In PV patients, JAK2V617F mutation burden had influence on WBC counts. And the clinical characteristics of ET patients, such as WBC counts, hemoglobin level, splenomegaly and thrombosis, were influenced by JAK2V617F mutation burden. Male, high hemoglobin (HGB above 150 g/L), and increased JAK2V617F mutation burden (JAK2V617F allele burden ≥ 16.5%) were risks of thrombosis (P< 0.05) for ET patients by Logistic Regression.
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.; 3. Department of Hematology, The First People's Hospital of Yunnan Province, No.154.Jinbi Road, Kunming, 650100, P.R China.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Feng
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.
authors.forename: Wenhong
authors.initials: W
authors.lastname: Sheng
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.
authors.forename: Jiannong
authors.initials: J
authors.lastname: Cen
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.
authors.forename: Depei
authors.initials: D
authors.lastname: Wu
authors.affiliation: 1. Department of Hematology, The First Affiliated Hospital of Soochow University, No.188.Shizi Street, Suzhou, 215006, P.R. China.; 2. Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, 215006, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
mesh_terms: D000368:Aged; D000483:Alleles; D002681:China; D005260:Female; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010641:Phenotype; D011087:Polycythemia Vera / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D013920:Thrombocythemia, Essential / Q000235:genetics* / Q000473:pathology; D013927:Thrombosis / Q000235:genetics / Q000473:pathology
chemical_list: C507924:JAK2 protein, human; D053614:Janus Kinase 2
keywords: Essential thrombocythemia; JAK2V671F mutation allele burden; Polycythemia vera; Thrombosis.