title: Chemical Mechanism of the Phosphotriesterase from Sphingobium sp. Strain TCM1, an Enzyme Capable of Hydrolyzing Organophosphate Flame Retardants.
abstract: The mechanism of action of the manganese-dependent phosphotriesterase from Sphingobium sp. strain TCM1 that is capable of hydrolyzing organophosphate flame retardants was determined. The enzyme was shown to hydrolyze the RP-enantiomer of O-methyl O-cyclohexyl p-nitrophenyl thiophosphate with net inversion of configuration and without the formation of a covalent reaction intermediate. These results demonstrate that the enzyme catalyzes the hydrolysis of substrates by activation of a nucleophilic water molecule for direct attack at the phosphorus center.
authors.affiliation: Department of Chemistry, Texas A&M University , College Station, Texas 77843, United States.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Bigley
authors.affiliation: Department of Chemistry, Texas A&M University , College Station, Texas 77843, United States.
authors.forename: Dao Feng
authors.initials: DF
authors.lastname: Xiang
authors.affiliation: Department of Biochemistry & Biophysics, Texas A&M University , College Station, Texas 77843, United States.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Department of Chemistry, Texas A&M University , College Station, Texas 77843, United States.
authors.forename: Haoran
authors.initials: H
authors.lastname: Xue
authors.affiliation: Department of Chemistry, Texas A&M University , College Station, Texas 77843, United States.
authors.forename: Kenneth G
authors.initials: KG
authors.lastname: Hull
authors.affiliation: Department of Chemistry, Texas A&M University , College Station, Texas 77843, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Romo
authors.affiliation: Department of Chemistry, Texas A&M University , College Station, Texas 77843, United States.|Department of Biochemistry & Biophysics, Texas A&M University , College Station, Texas 77843, United States.
authors.forename: Frank M
authors.initials: FM
authors.lastname: Raushel
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D005411:Flame Retardants / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D010755:Organophosphates / Q000737:chemistry* / Q000378:metabolism; D044345:Phosphoric Triester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D042301:Sphingomonadaceae / Q000201:enzymology*; D013237:Stereoisomerism
chemical_list: D001426:Bacterial Proteins; D005411:Flame Retardants; D010755:Organophosphates; D044345:Phosphoric Triester Hydrolases