title: Protein crystallography beamline (PX-BL21) at Indus-2 synchrotron.
abstract: The protein crystallography beamline (PX-BL21), installed at the 1.5 T bending-magnet port at the Indian synchrotron (Indus-2), is now available to users. The beamline can be used for X-ray diffraction measurements on a single crystal of macromolecules such as proteins, nucleic acids and their complexes. PX-BL21 has a working energy range of 5-20 keV for accessing the absorption edges of heavy elements commonly used for phasing. A double-crystal monochromator [Si(111) and Si(220)] and a pair of rhodium-coated X-ray mirrors are used for beam monochromatization and manipulation, respectively. This beamline is equipped with a single-axis goniometer, Rayonix MX225 CCD detector, fluorescence detector, cryogenic sample cooler and automated sample changer. Additional user facilities include a workstation for on-site data processing and a biochemistry laboratory for sample preparation. In this article the beamline, other facilities and some recent scientific results are briefly described.
authors.affiliation: High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
authors.forename: Ashwani
authors.initials: A
authors.lastname: Kumar
authors.affiliation: High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
authors.forename: Biplab
authors.initials: B
authors.lastname: Ghosh
authors.affiliation: High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
authors.forename: H K
authors.initials: HK
authors.lastname: Poswal
authors.affiliation: High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Pandey
authors.affiliation: Advanced Centre for Treatment, Research and Education in Cancer, Navi Mumbai, India.
authors.forename: M V
authors.initials: MV
authors.lastname: Hosur
authors.affiliation: High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
authors.forename: Abhilash
authors.initials: A
authors.lastname: Dwivedi
authors.affiliation: High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
authors.forename: Ravindra D
authors.initials: RD
authors.lastname: Makde
authors.affiliation: High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
authors.forename: Surinder M
authors.initials: SM
authors.lastname: Sharma
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D017356:Synchrotrons
chemical_list: D011506:Proteins
keywords: Indus-2; beamline; bending magnet; protein crystallography