title: [Difference of in vitro osteogenic differentiation and osteoclast capacity between stem cells from human exfoliated deciduous teeth and dental pulp stem cells].
abstract: OBJECTIVE
  To compare the osteogenic differentiation potential and osteoclast capacity between stem cells from human exfoliated deciduous teeth (SHED) in the physiological root resorption period and dental pulp stem cells (DPSCs).
  
  
  METHODS
  SHED and DPSCs were isolated, purified and cultured in vitro. The two stem cells were examined with ALP staining at 14 days and with alizarin red staining at 21 days of osteogenic induction, and the expressions of the genes associated with osteogenesis and osteoclastogenesis were detected using real-time PCR.
  
  
  RESULTS
  The isolated SHED and DPSCs both showed an elongate spindle-shaped morphology. After osteogenic induction of the cells, Alizarin red staining visualized a greater number of mineralized nodules in SHED than in DPSCs (P<0.05), and SHED also exhibited a stronger ALP activity than DPSCs (P<0.05). RT-PCR test results showed that the two stem cells expressed RANKL,OCN, ALP, OPG and Runx2 mRNA after osteogenic induction, but the expression levels of Runx2, OCN and ALP were lower in DPSCs than in SHED (P<0.05), and the ratio of RANKL/OPG was significantly higher in SHED (P<0.05).
  
  
  CONCLUSIONS
  Compared with DPSCs, SHED has not only the ability of osteogenic differentiation but also an osteoclast capacity, which sheds light on the regulatory role of SHED in physiological root resorption bone remodeling.
authors.affiliation: Department of Orthodontics, 2Institute of Oral Medicine, General Hospital of PLA, Beijing 100853, China. E-mail: 457838244@qq.com.
authors.forename: Bo-Wen
authors.initials: BW
authors.lastname: Lu
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Xu
authors.forename: Hai-Gang
authors.initials: HG
authors.lastname: Shi
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D010010:Osteoclasts / Q000166:cytology*; D010012:Osteogenesis; D053495:Osteopontin / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D014094:Tooth, Deciduous / Q000166:cytology*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D053245:RANK Ligand; C493709:RUNX2 protein, human; C506895:SPP1 protein, human; C505688:TNFSF11 protein, human; D053495:Osteopontin; D000469:Alkaline Phosphatase