title: Increased coagulation and fibrinolytic potential of solvent-detergent plasma: a comparative study between Omniplasma and fresh frozen plasma.
abstract: OBJECTIVE
  In this study, differences in levels of proteins involved in coagulation and fibrinolysis were compared between fresh frozen (quarantine plasma) and Omniplasma. Furthermore, thawing conditions and plasma stability after thawing were studied.
  
  
  METHODS
  10 Omniplasma and 10 quarantine plasma units were used to study different procoagulation, anticoagulation and fibrinolytic parameters. Analysis took place at different time-points during plasma storage at 2-6°C.
  
  
  RESULTS
  At baseline, significant reduced levels of factor V, free protein S, α2-antiplasmin and tPA-induced ROTEM lysis time were observed in Omniplasma as compared to quarantine plasma. Moreover, thrombin generation, IXa-AT complex levels and factor XIa were significantly increased in Omniplasma. The majority of the parameters studied remained stable in Omniplasma 48 h after thawing, with the exception of factor VIII (decrease) and IXa-AT (increase).
  
  
  CONCLUSIONS
  Our results suggest an increased coagulation potential, presumingly as a result of contact activation during the production process and also, an increased fibrinolytic potential in Omniplasma. The stability of Omniplasma, based upon the different parameters studied, is comparable to Q-plasma. A maximum post-thawing time of 48 hfor Omniplasma can be suggested.
authors.affiliation: Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: J J B C
authors.identifier: http://orcid.org/0000-0002-7865-5272
authors.initials: JJ
authors.lastname: van Beers
authors.affiliation: Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: L T
authors.initials: LT
authors.lastname: van Egmond
authors.affiliation: Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: R J H
authors.initials: RJ
authors.lastname: Wetzels
authors.affiliation: Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: P W M
authors.initials: PW
authors.lastname: Verhezen
authors.affiliation: Department of Internal Medicine, Subdivision Hematology, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: E A M
authors.initials: EA
authors.lastname: Beckers
authors.affiliation: Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center, Maastricht, the Netherlands.|Laboratory for Clinical Thrombosis and Haemostasis, Department of Internal Medicine, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: van Oerle
authors.affiliation: Laboratory for Clinical Thrombosis and Haemostasis, Department of Internal Medicine, Cardiovascular Research Institute Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: H M H
authors.initials: HM
authors.lastname: Spronk
authors.affiliation: Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: R J M H E
authors.initials: RJ
authors.lastname: Straat
authors.affiliation: Central Diagnostic Laboratory, Cluster for Hemostasis and Transfusion, Maastricht University Medical Center, Maastricht, the Netherlands.
authors.forename: Y M C
authors.initials: YM
authors.lastname: Henskens
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects*; D003902:Detergents / Q000737:chemistry / Q000494:pharmacology*; D015949:Factor IXa / Q000378:metabolism; D015945:Factor XIa / Q000378:metabolism; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D000979:alpha-2-Antiplasmin / Q000378:metabolism
chemical_list: D003902:Detergents; D012997:Solvents; D000979:alpha-2-Antiplasmin; D015949:Factor IXa; D015945:Factor XIa; D010959:Tissue Plasminogen Activator
keywords: Omniplasma; quarantine plasma; solvent-detergent plasma