title: Oriented bone regenerative capacity of octacalcium phosphate/gelatin composites obtained through two-step crystal preparation method.
abstract: The present study was designed to investigate whether composite of coprecipitating octacalcium phosphate and gelatin (C-OCP/Gel) has an effect in repairing critical-sized defect of rat calvaria with oriented regenerative bone if implanted. The materials were prepared through two steps to disperse homogenous and well-elongated OCP toward long axis of the crystals in gelatin (Gel) matrix with the distinct concentration 17-44 wt %: OCP precipitates recovered from the coprecipitated with Gel molecules in aqueous solution (referred to as C-OCP hereafter) were mixed again in fresh aqueous Gel solution with various mixing ratio to form C-OCP/Gel for implantation. C-OCP/Gel disks with 9 mm diameter and 1 mm thickness after the dehydrothermal treatment was implanted in 9 mm diameter rat calvaria critical-sized defect. The histology, the histomorphometry in the regenerated bone and the quantitative analysis of the orientation of collagen with picrosirius red staining were carried out. It was found that C-OCP/Gel is capable of not only inducing sufficiently regenerative bone over 80% of the defect coupled with practically complete material biodegradation but also forming oriented bone significantly in relation to the amount of C-OCP in Gel matrix until 12 weeks after the implantation. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1029-1039, 2017.
authors.affiliation: Division of Maxillofacial Prosthetic Clinic, Tohoku University Hospital, Sendai, 980-8575, Japan.|Division of Craniofacial Function Engineering, Tohoku University Graduate of Dentistry, Sendai, 980-8575, Japan.|Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Ishiko-Uzuka
authors.affiliation: Division of Craniofacial Function Engineering, Tohoku University Graduate of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Anada
authors.affiliation: Division of Craniofacial Function Engineering, Tohoku University Graduate of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Kobayashi
authors.affiliation: Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Kawai
authors.affiliation: Division of Oral and Maxillofacial Surgery, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tanuma
authors.affiliation: Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Division of Craniofacial Function Engineering, Tohoku University Graduate of Dentistry, Sendai, 980-8575, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D013552:Swine
chemical_list: D002130:Calcium Phosphates; C022045:octacalcium phosphate; D005780:Gelatin
keywords: coprecipitation; crystal growth; gelatin; octacalcium phosphate; oriented bone regeneration