title: Colistin Increases the Cidal Activity of Antibiotic Combinations Against Multidrug-Resistant Klebsiella pneumoniae: An In Vitro Model Comparing Multiple Combination Bactericidal Testing at One Peak Serum Concentration and Time-Kill Method.
abstract: The lack of treatment for multidrug-resistant (MDR) Enterobacteriaceae often leads to the use of double or triple antibiotic combinations to increase the option of clinical success. This study analyzes multiple combination bactericidal testing (MCBT) to screen double and triple antibiotic combinations, at standard peak serum concentration, for bactericidal activity against 21 MDR Klebsiella pneumoniae isolates. This method was compared with time-killing curves. The full bactericidal activity against all strains was obtained only by adding colistin. MCBT has a potential to become a rapid method for testing multiple antibiotic combinations for MDR microorganisms when colistin is used, providing clinicians with in vitro cidal data within 48 hr of strain isolation.
authors.affiliation: 1 Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania , Catania, Italy .
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Mezzatesta
authors.affiliation: 1 Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania , Catania, Italy .
authors.forename: Carla
authors.initials: C
authors.lastname: Caio
authors.affiliation: 2 Department of Laboratory Medicine and Advanced Biotechnologies, Mediterranean Institute for Transplantation and Advanced Specialized Therapies (ISMETT) , Palermo, Italy .
authors.forename: Floriana
authors.initials: F
authors.lastname: Gona
authors.affiliation: 1 Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania , Catania, Italy .
authors.forename: Tiziana
authors.initials: T
authors.lastname: Zingali
authors.affiliation: 1 Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania , Catania, Italy .
authors.forename: Iasmine
authors.initials: I
authors.lastname: Salerno
authors.affiliation: 1 Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania , Catania, Italy .
authors.forename: Stefania
authors.initials: S
authors.lastname: Stefani
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003091:Colistin / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D000077727:Ertapenem; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives / Q000494:pharmacology; D011237:Predictive Value of Tests; D012293:Rifampin / Q000494:pharmacology; D013845:Thienamycins / Q000494:pharmacology; D000078304:Tigecycline; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D013845:Thienamycins; D047090:beta-Lactams; D000078304:Tigecycline; D000077731:Meropenem; D008911:Minocycline; D000077727:Ertapenem; D012293:Rifampin; D003091:Colistin