title: [Role of SIRT3 in regulating proliferation of hepatocellular carcinoma cells in vitro].
abstract: OBJECTIVE
  To explore the role of SIRT3 in regulating the proliferation of hepatocellular carcinoma (HCC) cells in vitro.
  
  
  METHODS
  The protein expression of SIRT3 in 2 normal liver tissues, 2 immortalized hepatocyte lines, and 3 HCC cell lines was determined with Western blotting. SIRT3 overexpression and knockdown in HCC cells were induced by transfection with a vector expressing SIRT3 and a siRNA construct targeting SIRT3, respectively. The efficiency of SIRT3 overexpression and knockdown was detected by Western blot and qRT-PCR, respectively. The proliferation of the transfected HCC cells was examined using Trypan blue exclusion assay, and the cellular DNA synthesis was tested using EdU incorporation assay. The colony-forming ability of the cells was analyzed by colony formation assays.
  
  
  RESULTS
  SIRT3 expression was significantly lower in the 3 HCC cell lines than in immortalized hepatocytes and normal liver tissues. SIRT3 overexpression in HCC cells significantly lowered the cell proliferation by 51%-61% (P<0.001), reduced cellular DNA synthesis by 57% (P<0.05), and inhibited colony formation of the cells. SIRT3 knockdown significantly increased the proliferation of HCC cells by 51%-61% (P<0.01) and enhanced DNA synthesis by 137%-149% (P<0.01).
  
  
  CONCLUSIONS
  SIRT3 plays a inhibitory role in regulating the proliferation of HCC cells in vitro.
authors.affiliation: Department of Infectious Diseases, 161 Hospital of PLA, Wuhan 430012, China. E-mail: yingzi.1113@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tao
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering; D056566:Sirtuin 3 / Q000378:metabolism*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; C466093:SIRT3 protein, human; D056566:Sirtuin 3