title: Simple non-invasive analysis of embryonic stem cell-derived cardiomyocytes beating in vitro.
abstract: The analysis of digital video output enables the non-invasive screening of various active biological processes. For the monitoring and computing of the beating parameters of cardiomyocytes in vitro, CB Analyser (cardiomyocyte beating analyser) software was developed. This software is based on image analysis of the video recording of beating cardiomyocytes. CB Analyser was tested using cardiomyocytes derived from mouse embryonic stem cells at different stages of cardiomyogenesis. We observed that during differentiation (from day 18), the beat peak width decreased, which corresponded to the increased speed of an individual pulse. However, the beating frequency did not change. Further, the effects of epinephrine modulating mature cardiomyocyte functions were tested to validate the CB Analyser analysis. In conclusion, data show that CB Analyser is a useful tool for evaluating the functions of both developing and mature cardiomyocytes under various conditions in vitro.
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Katarzyna Anna
authors.initials: KA
authors.lastname: Radaszkiewicz
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Dominika
authors.initials: D
authors.lastname: Sýkorová
authors.affiliation: Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Karas
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Kudová
authors.affiliation: Research Center for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Kohút
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Lucia
authors.initials: L
authors.lastname: Binó
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Večeřa
authors.affiliation: Institute of Biophysics ASCR v.v.i., Brno, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Víteček
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Kubala
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Pacherník
mesh_terms: D000818:Animals; D002460:Cell Line; D004837:Epinephrine / Q000494:pharmacology*; D007091:Image Processing, Computer-Assisted; D051379:Mice; D018715:Microscopy, Video / Q000379:methods; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D012984:Software
chemical_list: D004837:Epinephrine