title: [Regulatory effect of curcumin on subsets and co-stimulatory molecules of dendritic cells in spleen from mice with colitis].
abstract: OBJECTIVE
  To explore the effect of curcumin (Cur) on the subpopulation and costimulatory molecules of dendritic cells (DCs) in spleen from colitis mice.
  
  
  METHODS
  Forty male C57/BL mice were randomly divided into 4 groups: normal group, model group, Cur group and mesalazine group. Colitis was induced by 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). On the 8th day following 7-day treatment with Cur, the mice were sacrificed and the length and mass of mouse colon were measured. And then the spleens were separated to prepare DCs. The numbers of CD205(+), CD4(+)CD205(+), CD8(+)CD205(+) DCs and the levels of major histocompability complex II (MHC-II), Toll-like receptor 2 (TLR2), TLR4 and CD83 expression were detected by flow cytometry. And we observed the pathological injury of colon.
  
  
  RESULTS
  Compared with model group, the colonic mass index was reduced, the length of colon was lengthened, and the pathological injury was remitted. Meanwhile, the percents of CD205(+), CD4(+)CD205(+), CD8(+)CD205(+) DCs and the levels of MHC-II, TLR2 and TLR4 were down-regulated, however, the CD83 expression was up-regulated.
  
  
  CONCLUSIONS
  Treatment efficacy of Cur on experimental colitis might be involved in regulating subpopulation and co-stimulatory molecules of dendritic cells in spleen from mice with colitis.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, Xiushui Hospital of Traditional Chinese Medicine, Jiujiang 332400, China.
authors.forename: Minfang
authors.initials: M
authors.lastname: Huang
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yue
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Duanyong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. *Corresponding author, E-mail: haimei79@163.com.
authors.forename: Haimei
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism*; D002478:Cells, Cultured; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D013154:Spleen / Q000473:pathology; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014302:Trinitrobenzenesulfonic Acid; D003474:Curcumin