title: Verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii Miq. inhibited the gene expression and production of MUC5AC mucin from human airway epithelial cells.
abstract: BACKGROUND
  The bulb of Fritillaria thunbergii has been utilised as mucoregulators and expectorants for controlling the airway inflammatory diseases in folk medicine.
  
  
  OBJECTIVE
  We investigated whether verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii inhibit the gene expression and production of MUC5AC mucin from human airway epithelial cells.
  
  
  METHODS
  Confluent NCI-H292 cells were pretreated with verticine, ebeiedine or suchengbeisine for 30 min and then stimulated with EGF, PMA or TNF-α for 24h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA.
  
  
  RESULTS
  (1) Verticine, ebeiedine or suchengbeisine inhibited the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-α; (2) The production of MUC5AC mucin protein induced by EGF, PMA or TNF-α were also inhibited by treatment of verticine, ebeiedine or suchengbeisine.
  
  
  CONCLUSIONS
  These results suggest that verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii inhibit the gene expression and production of MUC5AC mucin, by directly acting on airway epithelial cells, and the results are consistent with the traditional use of Fritillaria thunbergii as remedy for diverse inflammatory pulmonary diseases.
authors.affiliation: Department of Pediatrics, College of Korean Medicine, Dongguk University, Gyeongju, South Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, South Korea.
authors.forename: Yong Pill
authors.initials: YP
authors.lastname: Yoon
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Kyeong Wan
authors.initials: KW
authors.lastname: Woo
authors.affiliation: Department of Pediatrics, College of Korean Medicine, Dongguk University, Gyeongju, South Korea.
authors.forename: Jang-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Pediatrics, College of Korean Medicine, Dongguk University, Gyeongju, South Korea.
authors.forename: Sang Yeon
authors.initials: SY
authors.lastname: Min
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, South Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul, South Korea.
authors.forename: Sang Kook
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, South Korea.
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon, South Korea . Electronic address: krlee@skku.edu.
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, South Korea . Electronic address: LCJ123@cnu.ac.kr.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D002595:Cevanes / Q000737:chemistry* / Q000302:isolation & purification; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D031410:Fritillaria / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D055271:Mucin 5AC / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D002595:Cevanes; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D010936:Plant Extracts; D013256:Steroids; D014409:Tumor Necrosis Factor-alpha; C000611556:ebeiedine; C000588495:suchengbeisine; C014242:verticine; D004815:Epidermal Growth Factor
keywords: Airway; Ebeiedine; Mucin; Suchengbeisine; Verticine