title: Cost-effective and concurrent production of industrially valuable xylano-pectinolytic enzymes by a bacterial isolate Bacillus pumilus AJK.
abstract: Simultaneous production of xylanase and pectinase by Bacillus pumilus AJK under submerged fermentation was investigated in this study. Under optimized conditions, it produced 315 ± 16 IU/mL acidic xylanase, 290 ± 20 IU/mL alkaline xylanase, and 88 ± 9 IU/mL pectinase. The production of xylano-pectinolytic enzymes was the highest after inoculating media (containing 2% each of wheat bran and Citrus limetta peel, 0.5% peptone, 10 mM MgSO4, pH 7.0) with 2% of 21-hr-old culture and incubated at 37°C for 60 hr at 200 rpm. Xylanase retained 100% activity from pH 6.0 to10.0 after 3 hr of incubation, while pectinase showed 100% stability from pH 6.0 to 9.0 even after 6 hr of incubation. Cost-effective and concurrent production of xylanase and pectinase by a bacterial isolate in the same production media suggests its potential for various biotechnological applications. This is the first report of simultaneous production of industrially important extracellular xylano-pectinolytic enzymes by B. pumilus.
authors.affiliation: a Department of Biotechnology , Kurukshetra University , Kurukshetra , India.
authors.forename: Amanjot
authors.initials: A
authors.lastname: Kaur
authors.affiliation: a Department of Biotechnology , Kurukshetra University , Kurukshetra , India.
authors.forename: Avtar
authors.initials: A
authors.lastname: Singh
authors.affiliation: b Department of Biochemistry , University College, Kurukshetra University , Kurukshetra , India.
authors.forename: Anita
authors.initials: A
authors.lastname: Dua
authors.affiliation: a Department of Biotechnology , Kurukshetra University , Kurukshetra , India.
authors.forename: Ritu
authors.initials: R
authors.lastname: Mahajan
mesh_terms: D003362:Cost-Benefit Analysis; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000378:metabolism; D004795:Enzyme Stability; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010368:Pectins / Q000378:metabolism*; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014990:Xylans / Q000378:metabolism*
chemical_list: D014990:Xylans; D010368:Pectins; D011096:Polygalacturonase; D043364:Endo-1,4-beta Xylanases
keywords: Bacillus pumilus; Citrus limetta (mosambi) peel; submerged fermentation; wheat bran; xylano-pectinolytic enzymes