title: Anti-inflammatory neolignans from the roots of Magnolia officinalis.
abstract: Nine neolignan derivatives (1-9) were characterized from the roots of Magnolia officinalis, and their structures were elucidated based on spectroscopic and physicochemical analyses. Among them, houpulins E (1) and M (9) possess novel homo- and trinor-neolignan skeletons. In addition, 15 known compounds (10-24) were identified by comparison of their spectroscopic and physical data with those reported in the literature. Some of the purified constituents were examined for anti-inflammatory activity and, among the tested compounds, houpulins G (3), I (5), J (6), and 2,2'-dihydroxy-3-methoxy-5,5'-di-(2-propenylbiphenyl) (19) significantly inhibited superoxide anion generation and elastase release with IC50 values ranging from 3.54 to 5.48 μM and 2.16 to 3.39 μM, respectively. Therefore, these neolignan derivatives have tremendous potential to be explored as anti-inflammatory agents.
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC.
authors.forename: Hung-Cheng
authors.initials: HC
authors.lastname: Shih
authors.affiliation: Department of Biotechnology, National Formosa University, Yunlin 632, Taiwan, ROC.
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Kuo
authors.affiliation: Department of Medical Technology, Chung Hua University of Medical Technology, Tainan 717, Taiwan, ROC.
authors.forename: Shwu-Jen
authors.initials: SJ
authors.lastname: Wu
authors.affiliation: Graduate Institute of Natural Products, Chang Gung University, Taoyuan 333, Taiwan, ROC; Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan, ROC.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC.
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Hung
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC.
authors.forename: De-Yang
authors.initials: DY
authors.lastname: Shen
authors.affiliation: Department of Pharmacy, Tajen University, Pintung 907, Taiwan, ROC.
authors.forename: Po-Chuen
authors.initials: PC
authors.lastname: Shieh
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC.
authors.forename: Yu-Ren
authors.initials: YR
authors.lastname: Liao
authors.affiliation: Department of Surgery, and Institute of Biomedical Engineering, National Cheng Kung University, Medical Center and Medical School, Tainan 701, Taiwan, ROC; Department of Anesthesiology, and Institute of Biomedical Engineering, National Cheng Kung University, Medical Center and Medical School, Tainan 701, Taiwan, ROC.
authors.forename: E-Jian
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA; Chinese Medicine Research and Development Center, China Medical University and Hospital, Taichung 401, Taiwan, ROC. Electronic address: khlee@unc.edu.
authors.forename: Kuo-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.affiliation: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, ROC; Department of Pharmacy, Tajen University, Pintung 907, Taiwan, ROC. Electronic address: tswu@mail.ncku.edu.tw.
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031566:Magnolia / Q000737:chemistry*; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry*; D011789:Quantum Theory; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D017705:Lignans; D013481:Superoxides; D010196:Pancreatic Elastase
keywords: Anti-inflammatory activity; Houpulins E–M; Magnolia officinalis; Neolignans