title: Characterization of a novel Achromobacter xylosoxidans specific siphoviruse: phiAxp-1.
abstract: Bacteriophages have recently been considered as an alternative biocontrol tool because of the widespread occurrence of antimicrobial-resistant Achromobacter xylosoxidans. Herein, we isolated a virulent bacteriophage (phiAxp-1) from a water sample of the Bohai sea of China that specifically infects A. xylosoxidans. Transmission electron microscopy revealed that phage phiAxp-1 belongs to the Siphoviridae. We sequenced the genome of phiAxp-1, which comprises 45,045 bp with 64 open reading frames. Most of the proteins encoded by phiAxp-1 have no similarity to sequences in the public databases. Twenty-one proteins with assigned functions share weak homology with those of other dsDNA bacteriophages infecting diverse hosts, such as Burkholderia phage KL1, Pseudomonas phage 73, Pseudomonas phage vB_Pae-Kakheti25, Pseudomonas phage vB_PaeS_SCH_Ab26, Acinetobacter phage IME_AB3 and Achromobacter phage JWX. The genome can be divided into different clusters for the head and tail structure, DNA replication and mazG. The sequence and genomic organization of bacteriophage phiAxp-1 are clearly distinct from other known Siphoviridae phages; therefore, we propose that it is a member of a novel genus of the Siphoviridae family. Furthermore, one-step growth curve and stability studies of the phage were performed, and the specific receptor of phiAxp-1 was identified as the lipopolysaccharide of A. xylosoxidans.
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou, China, 510642.
authors.forename: Erna
authors.initials: E
authors.lastname: Li
authors.affiliation: Emergency Department, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China, 450052.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Food Science, Henan Institute of Science and Technology, Xinxiang, China, 453003.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China, 100071.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China, 100071.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China, 100071.
authors.forename: Weishi
authors.initials: W
authors.lastname: Lin
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China, 100071.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine, Tianjin, China, 300050.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine, Tianjin, China, 300050.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Shen
authors.affiliation: College of Food Science, Henan Institute of Science and Technology, Xinxiang, China, 453003.
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Zhao
authors.affiliation: College of Food Science, South China Agricultural University, Guangzhou, China, 510642.
authors.forename: Aimin
authors.initials: A
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China, 100071.
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China, 100071.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China, 100071.
authors.forename: Xiangna
authors.initials: X
authors.lastname: Zhao
mesh_terms: D042441:Achromobacter denitrificans / Q000821:virology*; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D016679:Genome, Viral; D008070:Lipopolysaccharides / Q000378:metabolism*; D005810:Multigene Family; D016366:Open Reading Frames; D011991:Receptors, Virus / Q000378:metabolism*; D012623:Seawater / Q000821:virology; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D004279:DNA, Viral; D008070:Lipopolysaccharides; D011991:Receptors, Virus