title: Comparative analysis of microRNA expression in human mesenchymal stem cells from umbilical cord and cord blood.
abstract: Human mesenchymal stem cells (MSCs) derived from both umbilical cord (UC) and cord blood (CB) share similar characteristics, and their differences are largely unknown. Besides the significant difference in cell morphology, differentiation ability and development processes of the two different origin MSCs, a different expression pattern of microRNAs between the two kinds of MSCs was also obtained. By comprehensively annotating the differently expressed global microRNAs (miRNAs), a series of biological pathways were predicted. We found that miRNAs significantly repressed insulin signaling in UCMSCs, while neural related processes were more repressed in CBMSCs. Particularly, TGF-β and Notch signaling were differently activated in both MSCs, unveiling their distinct angiogenesis potentials. Taken together, this study illustrates that MSCs from UC and CB display distinct properties, which indicates different potentials for clinical usage.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
authors.forename: Xianhui
authors.initials: X
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: sunbo@seu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: zdxiao@seu.edu.cn.
authors.forename: Zhongdang
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D050156:Adipogenesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D019295:Computational Biology; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D007328:Insulin / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D010012:Osteogenesis; D051880:Receptors, Notch; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D007328:Insulin; D035683:MicroRNAs; D051880:Receptors, Notch; D016212:Transforming Growth Factor beta
keywords: Cord blood; Mesenchymal stem cells; MicroRNA; Umbilical cord