title: Molecularly Imprinted Electropolymer for a Hexameric Heme Protein with Direct Electron Transfer and Peroxide Electrocatalysis.
abstract: For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of -184.4 ± 13.7 mV vs. Ag/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higher electroactive surface concentration of HTHP than the non-imprinted polymer (NIP).
authors.affiliation: Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany. penglei0525@163.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.affiliation: Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany. aysu.yarman@yahoo.de.
authors.forename: Aysu
authors.initials: A
authors.lastname: Yarman
authors.affiliation: Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany. jetzschm@uni-potsdam.de.
authors.forename: Katharina J
authors.initials: KJ
authors.lastname: Jetzschmann
authors.affiliation: Institute of Biology, Structural Biology/Biochemistry, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany. jae-hun.jeoung@biologie.hu-berlin.de.
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Jeoung
authors.affiliation: Institute of Biology, Structural Biology/Biochemistry, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany. schad.daniel@googlemail.com.
authors.forename: Daniel
authors.initials: D
authors.lastname: Schad
authors.affiliation: Institute of Biology, Structural Biology/Biochemistry, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany. holger.dobbek@biologie.hu-berlin.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Dobbek
authors.affiliation: Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany. uwollen@uni-potsdam.de.
authors.forename: Ulla
authors.initials: U
authors.lastname: Wollenberger
authors.affiliation: Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany. fschell@uni-potsdam.de.|Fraunhofer Institute for Cell Therapy and Immunology IZI-BB, Am Mühlenberg 13, 14476 Potsdam, Germany. fschell@uni-potsdam.de.
authors.forename: Frieder W
authors.initials: FW
authors.lastname: Scheller
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004566:Electrodes; D004579:Electron Transport; D006046:Gold / Q000737:chemistry; D006420:Hemeproteins / Q000737:chemistry / Q000302:isolation & purification*; D054802:Molecular Imprinting; D010545:Peroxides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties
chemical_list: D006420:Hemeproteins; D010545:Peroxides; D011108:Polymers; D006046:Gold
keywords: bioelectrocatalysis; direct electron transfer; hydrogen peroxide; molecularly imprinted polymers; self-assembled monolayer