title: Maturing Glycoproteomics Technologies Provide Unique Structural Insights into the N-glycoproteome and Its Regulation in Health and Disease.
abstract: The glycoproteome remains severely understudied because of significant analytical challenges associated with glycoproteomics, the system-wide analysis of intact glycopeptides. This review introduces important structural aspects of protein N-glycosylation and summarizes the latest technological developments and applications in LC-MS/MS-based qualitative and quantitative N-glycoproteomics. These maturing technologies provide unique structural insights into the N-glycoproteome and its synthesis and regulation by complementing existing methods in glycoscience. Modern glycoproteomics is now sufficiently mature to initiate efforts to capture the molecular complexity displayed by the N-glycoproteome, opening exciting opportunities to increase our understanding of the functional roles of protein N-glycosylation in human health and disease.
authors.affiliation: From the ‡Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia; morten.andersen@mq.edu.au.
authors.forename: Morten
authors.identifier: 0000-0001-8327-6843
authors.initials: M
authors.lastname: Thaysen-Andersen
authors.affiliation: From the ‡Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia;
authors.forename: Nicolle H
authors.initials: NH
authors.lastname: Packer
authors.affiliation: §School of Chemistry & Molecular Biosciences, St Lucia, The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Schulz
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000032:analysis*; D006031:Glycosylation; D006801:Humans; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D006023:Glycoproteins