title: An in vitro dynamic microcosm biofilm model for caries lesion development and antimicrobial dose-response studies.
abstract: Some dynamic biofilm models for dental caries development are limited as they require multiple experiments and do not allow independent biofilm growth units, making them expensive and time-consuming. This study aimed to develop and test an in vitro dynamic microcosm biofilm model for caries lesion development and for dose-response to chlorhexidine. Microcosm biofilms were grown under two different protocols from saliva on bovine enamel discs for up to 21 days. The study outcomes were as follows: the percentage of enamel surface hardness change, integrated hardness loss, and the CFU counts from the biofilms formed. The measured outcomes, mineral loss and CFU counts showed dose-response effects as a result of the treatment with chlorhexidine. Overall, the findings suggest that biofilm growth for seven days with 0.06 ml min(-1) salivary flow under exposure to 5% sucrose (3 × daily, 0.25 ml min(-1), 6 min) was suitable as a pre-clinical model for enamel demineralization and antimicrobial studies.
authors.affiliation: a Graduate Program in Dentistry , Federal University of Pelotas , Pelotas-RS , Brazil.
authors.forename: T T
authors.initials: TT
authors.lastname: Maske
authors.affiliation: a Graduate Program in Dentistry , Federal University of Pelotas , Pelotas-RS , Brazil.
authors.forename: K V
authors.initials: KV
authors.lastname: Brauner
authors.affiliation: a Graduate Program in Dentistry , Federal University of Pelotas , Pelotas-RS , Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Nakanishi
authors.affiliation: b Department of Preventive and Community Dentistry , Federal University of Rio Grande do Sul , Porto Alegre-RS , Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Arthur
authors.affiliation: a Graduate Program in Dentistry , Federal University of Pelotas , Pelotas-RS , Brazil.|c School of Dentistry , IMED Faculdade Meridional , Passo Fundo-RS , Brazil.
authors.forename: F H
authors.initials: FH
authors.lastname: van de Sande
authors.affiliation: a Graduate Program in Dentistry , Federal University of Pelotas , Pelotas-RS , Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Cenci
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002417:Cattle; D002710:Chlorhexidine / Q000494:pharmacology*; D015169:Colony Count, Microbial / Q000379:methods; D003731:Dental Caries / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology / Q000517:prevention & control; D003743:Dental Enamel / Q000187:drug effects / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D006245:Hardness Tests / Q000379:methods; D006801:Humans; D008954:Models, Biological; D009067:Mouthwashes / Q000494:pharmacology; D012463:Saliva / Q000382:microbiology; D017001:Tooth Demineralization / Q000175:diagnosis / Q000517:prevention & control
chemical_list: D009067:Mouthwashes; D002710:Chlorhexidine
keywords: Biofilm model; artificial dental caries; chlorhexidine; enamel demineralization