title: Effect of Minocycline on the Durability of Dentin Bonding Produced with Etch-and-Rinse Adhesives.
abstract: OBJECTIVE
  To evaluate the effect of minocycline and chlorhexidine pretreatment of acid-etched dentin on the longevity of resin-dentin bond strength (μTBS) and nanoleakage of two-step etch-and-rinse adhesives.
  
  
  METHODS
  Before application of Prime & Bond NT and Adper Single Bond 2 in occlusal dentin, the dentin surfaces were treated with 37% phosphoric acid, rinsed, air-dried, and rewetted with water (control group), 2% minocycline, or 2% chlorexidine digluconate. Composite buildups were constructed incrementally, and specimens were longitudinally sectioned to obtain bonded sticks (0.8 mm2) to be tested in tension (0.5 mm/min) immediately or after 24 months of water storage. For nanoleakage, two specimens of each tooth/period were immersed in the silver nitrate solution, photo-developed, and polished with SiC paper for analysis under energy-dispersive X-ray spectroscopy/scanning electron microscopy.
  
  
  RESULTS
  Reductions of the μTBS and increases in the nanoleakage were observed for both adhesives when the rewetting procedure was performed with water. Stable bonds were observed for the 2% minocycline and 2% chlorexidine digluconate groups after 24 months.
  
  
  CONCLUSIONS
  The use of 2% minocycline as pretreatment of acid-etched dentin is one alternative to retard the degradation of resin-dentin interfaces over a 24-month period as well as 2% chlorexidine digluconate.
authors.forename: A D
authors.initials: AD
authors.lastname: Loguercio
authors.forename: R
authors.initials: R
authors.lastname: Stanislawczuk
authors.forename: P
authors.initials: P
authors.lastname: Malaquias
authors.forename: M F
authors.initials: MF
authors.lastname: Gutierrez
authors.forename: J
authors.initials: J
authors.lastname: Bauer
authors.forename: A
authors.initials: A
authors.lastname: Reis