title: Influence of heat treatment on the structure and core IgE-binding epitopes of rAra h 2.02.
abstract: The rAra h 2.02 was studied to determine the influence of heat treatment on its structure and core IgE-binding epitopes. The results of SDS-PAGE, Western blotting, MALDI-TOF-MS, and atomic force microscopy showed that the structure of rAra h 2.02 was altered after boiling (100°C) or autoclaving (121°C) for 20min. Furthermore, some of the protein may be aggregated. Results of circular dichroism spectroscopy showed that the α-helices content was reduced, while β-turns and random coils were increased by 81% and 27%, respectively, after autoclaving. Antibodies of three core IgE-binding epitopes were used to determine the binding capacity of rAra h 2.02 after thermal processing by indirect ELISA. The results showed that the binding capacities of the three core IgE-binding epitopes were changed after different heat treatments.
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China; Shanghai Genechem Co., Ltd., Shanghai, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China.
authors.forename: Wen-Ju
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Zhu
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China. Electronic address: chenqincc@staff.shu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry* / Q000276:immunology; D052179:Antigens, Plant / Q000737:chemistry* / Q000276:immunology; D000939:Epitopes / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology; D006358:Hot Temperature; D007073:Immunoglobulin E / Q000276:immunology*; D018625:Microscopy, Atomic Force; D011994:Recombinant Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D055516:2S Albumins, Plant; D052179:Antigens, Plant; C500890:Ara h 2 allergen, Arachis hypogaea; D000939:Epitopes; D006023:Glycoproteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E
keywords: Allergen; Core IgE-binding epitope; Heat treatment; Peanut (Arachis hypogaea); rAra h 2.02