title: Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation.
abstract: Directional cell movements during morphogenesis require the coordinated interplay between membrane receptors and the actin cytoskeleton. The WAVE regulatory complex (WRC) is a conserved actin regulator. Here, we found that the atypical cadherin Fat2 recruits the WRC to basal membranes of tricellular contacts where a new type of planar-polarized whip-like actin protrusion is formed. Loss of either Fat2 function or its interaction with the WRC disrupts tricellular protrusions and results in the formation of nonpolarized filopodia. We provide further evidence for a molecular network in which the receptor tyrosine phosphatase Dlar interacts with the WRC to couple the extracellular matrix, the membrane, and the actin cytoskeleton during egg elongation. Our data uncover a mechanism by which polarity information can be transduced from a membrane receptor to a key actin regulator to control collective follicle cell migration during egg elongation. 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechanism driving rotational motions in epithelial morphogenesis.
authors.affiliation: Institute for Neurobiology, University of Münster, 48149 Münster, Germany.
authors.forename: Anna Julia
authors.initials: AJ
authors.lastname: Squarr
authors.affiliation: Institute for Neurobiology, University of Münster, 48149 Münster, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Brinkmann
authors.affiliation: Department of Biophysics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Chen
authors.affiliation: Institute-associated Research Group "Cell adhesion and cell polarity," University of Münster, 48149 Münster, Germany Institute of Medical Biochemistry, University of Münster, 48149 Münster, Germany Center for Molecular Biology of Inflammation, University of Münster, 48149 Münster, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Steinbacher
authors.affiliation: Institute-associated Research Group "Cell adhesion and cell polarity," University of Münster, 48149 Münster, Germany Institute of Medical Biochemistry, University of Münster, 48149 Münster, Germany Center for Molecular Biology of Inflammation, University of Münster, 48149 Münster, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Ebnet
authors.affiliation: Department of Biophysics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Rosen
authors.affiliation: Institute for Neurobiology, University of Münster, 48149 Münster, Germany sbogdan@uni-muenster.de.
authors.forename: Sven
authors.initials: S
authors.lastname: Bogdan
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002465:Cell Movement; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism*; D012399:Rotation
chemical_list: D000199:Actins; D015820:Cadherins; D029721:Drosophila Proteins; D046912:Multiprotein Complexes; C546978:kug protein, Drosophila