title: Toward Cell Selective Surfaces: Cell Adhesion and Proliferation on Breath Figures with Antifouling Surface Chemistry.
abstract: We report the preparation of microporous functional polymer surfaces that have been proven to be selective surfaces toward eukaryotic cells while maintaining antifouling properties against bacteria. The fabrication of functional porous films has been carried out by the breath figures approach that allowed us to create porous interfaces with either poly(ethylene glycol) methyl ether methacrylate (PEGMA) or 2,3,4,5,6-pentafluorostyrene (5FS). For this purpose, blends of block copolymers in a polystyrene homopolymer matrix have been employed. In contrast to the case of single functional polymer, using blends enables us to vary the chemical distribution of the functional groups inside and outside the formed pores. In particular, fluorinated groups were positioned at the edges while the hydrophilic PEGMA groups were selectively located inside the pores, as demonstrated by TOF-SIMS. More interestingly, studies of cell adhesion, growth, and proliferation on these surfaces confirmed that PEGMA functionalized interfaces are excellent candidates to selectively allow cell growth and proliferation while maintaining antifouling properties.
authors.affiliation: Tissue Engineering Group, Instituto de Estudios Biofuncionales, Universidad Complutense de Madrid , Paseo Juan XXIII, no. 1 28040 Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Martínez-Campos
authors.affiliation: Lebanese Atomic Energy Commission, National Council for Scientific Research CNRS-L , P.O. Box 11-8281, Riad El Solh, 1107 2260, Beirut, Lebanon.
authors.forename: Tamara
authors.initials: T
authors.lastname: Elzein
authors.affiliation: Lebanese Atomic Energy Commission, National Council for Scientific Research CNRS-L , P.O. Box 11-8281, Riad El Solh, 1107 2260, Beirut, Lebanon.
authors.forename: Alice
authors.initials: A
authors.lastname: Bejjani
authors.affiliation: Tissue Engineering Group, Instituto de Estudios Biofuncionales, Universidad Complutense de Madrid , Paseo Juan XXIII, no. 1 28040 Madrid, Spain.
authors.forename: Maria Jesús
authors.initials: MJ
authors.lastname: García-Granda
authors.affiliation: Tissue Engineering Group, Instituto de Estudios Biofuncionales, Universidad Complutense de Madrid , Paseo Juan XXIII, no. 1 28040 Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Santos-Coquillat
authors.affiliation: Tissue Engineering Group, Instituto de Estudios Biofuncionales, Universidad Complutense de Madrid , Paseo Juan XXIII, no. 1 28040 Madrid, Spain.
authors.forename: Viviana
authors.initials: V
authors.lastname: Ramos
authors.affiliation: Departamento de Química Física Aplicada, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Francisco Tomás y Valiente 7, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Muñoz-Bonilla
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (ICTP), Consejo Superior de Investigaciones Científicas (CSIC) , C/Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Rodríguez-Hernández
mesh_terms: D001422:Bacterial Adhesion; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000254:growth & development*; D020058:Styrene / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D006845:Hydrocarbons, Fluorinated; D008689:Methacrylates; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D020058:Styrene
keywords: antifouling surfaces; breath figures; functional polymer surfaces; microstructures; porous films; selective cell adhesive