title: Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries.
abstract: The fecal microbiota of six mice derived from three Japanese commercial breeders was analyzed by using 16S rRNA gene clone libraries to construct a database for analyzing the gut microbiota of laboratory mice. The 566 clones were obtained from the clone libraries generated from the fecal DNA samples derived from BALB/c, C57BL/6N, DBA/2 and ICR mice. Among these 566 clones, there were 446 unique 16S rRNA gene sequences. When grouped at the 98% similarity level, the 446 unique sequences consisted of 103 Clostridiales, 43 Bacteroidales, 5 Lactobacillus and 3 Erysipelotricaceae, as well as sequences from 11 other phyla.
authors.affiliation: ICLAS Monitoring Center, Central Institute for Experimental Animals, 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Nozu
authors.forename: Masami
authors.initials: M
authors.lastname: Ueno
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Hayashimoto
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005243:Feces / Q000382:microbiology*; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D015698:Genomic Library; D008297:Male; D051379:Mice / Q000382:microbiology; D008807:Mice, Inbred BALB C / Q000382:microbiology; D008810:Mice, Inbred C57BL / Q000382:microbiology; D008811:Mice, Inbred DBA / Q000382:microbiology; D008813:Mice, Inbred ICR / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S