title: Controlled Angiogenesis in Peptide Nanofiber Composite Hydrogels.
abstract: Multidomain peptide (MDP) nanofibers create scaffolds that can present bioactive cues to promote biological responses. Orthogonal self-assembly of MDPs and growth-factor-loaded liposomes generate supramolecular composite hydrogels. These composites can act as delivery vehicles with time-controlled release. Here we examine the controlled release of placental growth factor-1 (PlGF-1) for its ability to induce angiogenic responses. PlGF-1 was loaded either in MDP matrices or within liposomes bound inside MDP matrices. Scaffolds showed expected rapid infiltration of macrophages. When released through liposomes incorporated in MDP gels (MDP(Lipo)), PlGF-1 modulates HUVEC VEGF receptor activation in vitro and robust vessel formation in vivo. These loaded MDP(Lipo) hydrogels induce a high level of growth-factor-mediated neovascular maturity. MDP(Lipo) hydrogels offer a biocompatible and injectable platform to tailor drug delivery and treat ischemic tissue diseases.
authors.affiliation: Department of Chemistry, Rice University, Bioscience Research Collaborative, 6500 Main Street, Houston, Texas 77030, United States.
authors.forename: Navindee C
authors.initials: NC
authors.lastname: Wickremasinghe
authors.affiliation: Department of Chemistry, Rice University, Bioscience Research Collaborative, 6500 Main Street, Houston, Texas 77030, United States.
authors.forename: Vivek A
authors.initials: VA
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, Rice University, Bioscience Research Collaborative, 6500 Main Street, Houston, Texas 77030, United States.
authors.forename: Siyu
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Chemistry, Rice University, Bioscience Research Collaborative, 6500 Main Street, Houston, Texas 77030, United States; Department of Bioengineering, Rice University, Bioscience Research Collaborative, 6500 Main Street, Houston, Texas 77030, United States.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Hartgerink
keywords: PlGF-1; angiogenesis; cellular infiltration; liposomal encapsulation; multidomain peptide; self-assembly