title: Fragmented Lactic Acid Bacterial Cells Activate Peroxisome Proliferator-Activated Receptors and Ameliorate Dyslipidemia in Obese Mice.
abstract: Recent studies suggest that peroxisome proliferator-activated receptor (PPAR) activation ameliorates metabolic disorders, including dyslipidemia. To identify an effective PPAR agonist, we screened the in vitro PPARα/γ activation ability of organic solvent extracts from food-oriented bacterial strains belonging to 5 genera and 32 species, including lactic acid bacteria, and of these, Lactobacillus amylovorus CP1563 demonstrated the highest PPARα/γ agonist activity. We also found that physical fragmentation of the strain could substitute organic solvent extraction for the expression of CP1563 activity in vitro. For functional food manufacturing, we selected the fragmented CP1563 and conducted subsequent animal experiments. In an obese mouse model, we found that treatment with fragmented CP1563 for 12 weeks decreased the levels of low-density lipoprotein (LDL)-cholesterol and triglyceride in plasma, significantly decreased the atherosclerosis index, and increased the plasma high-density lipoprotein (HDL)-cholesterol level. Thus, we conclude that fragmented CP1563 may be a candidate for the prevention and treatment of dyslipidemia.
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd. , 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Nakamura
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd. , 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ishida
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd. , 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sawada
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd. , 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Ashida
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd. , 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
authors.forename: Tomonori
authors.initials: T
authors.lastname: Sugawara
authors.affiliation: Laboratory of Molecular Function of Food Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University , Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Sakai
authors.affiliation: Laboratory of Molecular Function of Food Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University , Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.affiliation: Laboratory of Molecular Function of Food Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University , Gokasho, Uji, Kyoto 611-0011, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawada
authors.affiliation: Research & Development Center, Asahi Group Holdings, Ltd. , 5-11-10 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-0206, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood; D050171:Dyslipidemias / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology; D019344:Lactic Acid / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D051379:Mice; D008820:Mice, Obese; D009765:Obesity / Q000097:blood; D047493:PPAR alpha / Q000819:agonists*; D047495:PPAR gamma / Q000819:agonists*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D047493:PPAR alpha; D047495:PPAR gamma; D014280:Triglycerides; D019344:Lactic Acid
keywords: cell fragmentation; dyslipidemia; lactic acid bacteria; peroxisome proliferator-activated receptors