title: Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China.
abstract: Trueperella (T.) pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. In this work, the properties, pathogenesis and phenotypic diversity of T. pyogenes isolates from bovine mastitis were studied. Both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by PCR in all T. pyogenes isolates (n = 50). Using the tissue culture plate method, 90% of T. pyogenes isolates were able to form biofilms. The minimum inhibitory concentrations (MICs) of 13 antimicrobials against T. pyogenes isolates were determined. High susceptibility was observed to rifampin (96%), ampicillin (94%), ciprofloxacin (94%), and penicillin (92%), while low susceptibility was found to trimethoprim-sulphamethoxazole (10%) and bacitracin (2%). The intracellular assay revealed that T. pyogenes isolates had different cytopathogenic effects on cells. The high percentage (28.6%) of T. pyogenes isolates suggests that this bacterium is an important contributor to mastitis. Moreover, the high occurrence of multidrug resistance, biofilm production, intracellular survival, and the temporal dynamics of T. pyogenes interactions are key factors for a better understanding of how immunity acts on infections with these bacteria and how they evade immune surveillance, thus highlighting the need for the prudent use of antimicrobial agents in veterinary medicine.
authors.affiliation: College of Veterinary Medicine, China Agricultural University , No. 2 Yuan Ming Yuan West Road, Haidian District, 100193 Beijing , PR China.
authors.forename: Rashad
authors.initials: R
authors.lastname: Alkasir
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture , Beijing , PR China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, China Agricultural University , No. 2 Yuan Ming Yuan West Road, Haidian District, 100193 Beijing , PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, China Agricultural University , No. 2 Yuan Ming Yuan West Road, Haidian District, 100193 Beijing , PR China.
authors.forename: Tariq
authors.initials: T
authors.lastname: Ali
authors.affiliation: College of Veterinary Medicine, China Agricultural University , No. 2 Yuan Ming Yuan West Road, Haidian District, 100193 Beijing , PR China.
authors.forename: Limei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department and Clinic for Production Animals, Faculty of Veterinary Sciences, Szent István University , Üllő, Dóra major , Hungary.|MTA-SZIE Large Animal Clinical Research Group , Üllő, Dóra major , Hungary.
authors.forename: Ottó
authors.initials: O
authors.lastname: Szenci
authors.affiliation: Department and Clinic for Production Animals, Faculty of Veterinary Sciences, Szent István University , Üllő, Dóra major , Hungary.|MTA-SZIE Large Animal Clinical Research Group , Üllő, Dóra major , Hungary.
authors.forename: Árpád Csaba
authors.initials: ÁC
authors.lastname: Bajcsy
authors.affiliation: College of Veterinary Medicine, China Agricultural University , No. 2 Yuan Ming Yuan West Road, Haidian District, 100193 Beijing , PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Han
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology* / Q000662:veterinary; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D055667:Arcanobacterium / Q000187:drug effects*; D002417:Cattle; D002681:China; D015169:Colony Count, Microbial; D024881:Drug Resistance, Bacterial; D005260:Female; D008414:Mastitis, Bovine / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D037521:Virulence Factors
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors
keywords: Trueperella pyogenes; antimicrobial susceptibility; biofilm; bovine mastitis; virulence factor