title: Group 3 innate lymphoid cells regulate neutrophil migration and function in human decidua.
abstract: Innate lymphoid cells (ILCs) have a central role in innate defenses against pathogens, lymphoid organogenesis, and tissue remodeling. They have been detected in human decidua, however, their role in this tissue remains unclear. Successful pregnancy requires an early inflammatory phase favoring implantation and tissue remodeling as well as a subsequent regulatory phase to prevent fetal rejection and supporting neoangiogenesis. Here, we show that, during the first trimester of pregnancy, neutrophils infiltrate decidua basalis and are more abundant in normal pregnancy than in spontaneous miscarriages. Decidual neutrophils localize in proximity of NCR+ILC3, which may influence neutrophil migration and survival given their production of CXCL8 and granulocyte macrophage colony-stimulating factor (GM-CSF). Moreover, NCR+ILC3-derived GM-CSF was found to induce the expression of heparin-binding EGF-like growth factor and IL1ra in neutrophils, two proteins/cytokines involved in tissue remodeling and maintenance of pregnancy. Our data suggest that the simultaneous presence of NCR+ILC3 and neutrophils in decidual tissues and their possible cross talk, may have a role in the early phases of pregnancy.
authors.affiliation: Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Croxatto
authors.affiliation: Department of Medicine, Section of General Pathology, School of Medicine, University of Verona, Verona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Micheletti
authors.affiliation: G. Gaslini Institute, Genoa, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Montaldo
authors.affiliation: Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Orecchia
authors.affiliation: G. Gaslini Institute, Genoa, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Loiacono
authors.affiliation: G. Gaslini Institute, Genoa, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Canegallo
authors.affiliation: Department of Medicine, Section of General Pathology, School of Medicine, University of Verona, Verona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Calzetti
authors.affiliation: G. Gaslini Institute, Genoa, Italy.|Department of Scienze Chirurgiche e Diagnostiche integrate (DISC), University of Genoa, Genoa, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Fulcheri
authors.affiliation: Department of Public Health and Community Medicine, Section of Pathological Anatomy, School of Medicine, University of Verona, Verona, Italy.|Department of Pathology Sacro Cuore Hospital, Verona, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Munari
authors.affiliation: Department of Public Health and Community Medicine, Section of Pathological Anatomy, School of Medicine, University of Verona, Verona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Zamò
authors.affiliation: IRCCS AOU San Martino-IST, Genoa, Italy.|Department of Neuroscienze, Riabilitazione, Oftalmologia, Genetica e Scienze Materno-infantili, University of Genoa, Genoa, Italy.
authors.forename: P L
authors.initials: PL
authors.lastname: Venturini
authors.affiliation: Ospedale Pediatrico Bambino Gesù, Viale Ferdinando Baldelli, Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Moretta
authors.affiliation: Department of Medicine, Section of General Pathology, School of Medicine, University of Verona, Verona, Italy.
authors.forename: M A
authors.initials: MA
authors.lastname: Cassatella
authors.affiliation: Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy.|IRCCS AOU San Martino-IST, Genoa, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Mingari
authors.affiliation: Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Vacca
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers; D002470:Cell Survival / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D003656:Decidua / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D066257:Heparin-binding EGF-like Growth Factor / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007113:Immunity, Innate; D008213:Lymphocyte Activation / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D020556:Neutrophil Infiltration / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism*; D010641:Phenotype; D011247:Pregnancy; D011261:Pregnancy Trimester, First
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D016207:Cytokines; D066257:Heparin-binding EGF-like Growth Factor