title: Light-controlled intracellular transport in Caenorhabditis elegans.
abstract: To establish and maintain their complex morphology and function, neurons and other polarized cells exploit cytoskeletal motor proteins to distribute cargoes to specific compartments. Recent studies in cultured cells have used inducible motor protein recruitment to explore how different motors contribute to polarized transport and to control the subcellular positioning of organelles. Such approaches also seem promising avenues for studying motor activity and organelle positioning within more complex cellular assemblies, but their applicability to multicellular in vivo systems has so far remained unexplored. Here, we report the development of an optogenetic organelle transport strategy in the in vivo model system Caenorhabditis elegans. We demonstrate that movement and pausing of various organelles can be achieved by recruiting the proper cytoskeletal motor protein with light. In neurons, we find that kinesin and dynein exclusively target the axon and dendrite, respectively, revealing the basic principles for polarized transport. In vivo control of motor attachment and organelle distributions will be widely useful in exploring the mechanisms that govern the dynamic morphogenesis of cells and tissues, within the context of a developing animal.
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: Harterink
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Petra
authors.initials: P
authors.lastname: van Bergeijk
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Calixte
authors.initials: C
authors.lastname: Allier
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Bart
authors.initials: B
authors.lastname: de Haan
authors.affiliation: Developmental Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Sander
authors.initials: S
authors.lastname: van den Heuvel
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands. Electronic address: c.hoogenraad@uu.nl.
authors.forename: Casper C
authors.initials: CC
authors.lastname: Hoogenraad
authors.affiliation: Cell Biology, Faculty of Science, Utrecht University, 3584 CH, Utrecht, The Netherlands. Electronic address: l.kapitein@uu.nl.
authors.forename: Lukas C
authors.initials: LC
authors.lastname: Kapitein
mesh_terms: D000818:Animals; D001692:Biological Transport; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D004398:Dyneins / Q000737:chemistry / Q000378:metabolism*; D016547:Kinesins / Q000737:chemistry / Q000378:metabolism*; D008027:Light; D062308:Optogenetics; D015388:Organelles / Q000737:chemistry / Q000378:metabolism*; D055503:Protein Multimerization
chemical_list: D004398:Dyneins; D016547:Kinesins