title: Milk and blood biomarkers associated to the clinical efficacy of a probiotic for the treatment of infectious mastitis.
abstract: Previous studies have shown the efficacy of oral administration of selected lactobacilli strains to treat mastitis. The objective of this study was to find microbiological, biochemical and/or immunological biomarkers of the probiotic effect. Women with (n=23) and without (n=8) symptoms of mastitis received three daily doses (10(9) cfu) of Lactobacillus salivarius PS2 for 21 days. Samples of milk, blood and urine were collected before and after the probiotic intervention, and screened for a wide spectrum of microbiological, biochemical and immunological parameters. In the mastitis group, L. salivarius PS2 intake led to a reduction in milk bacterial counts, milk and blood leukocyte counts and interleukin (IL)-8 level in milk, an increase in those of immunoglobulin (Ig)E, IgG3, epidermal growth factor and IL-7, a modification of the milk electrolyte profile, and a reduction of some oxidative stress biomarkers. Such biomarkers will be useful in future clinical studies involving a larger cohort.
authors.affiliation: 1 Department of Nutrition, Food Science and Food Technology, Complutense University of Madrid, Av. Puerta de Hierro, s/n, 28040 Madrid, Spain.|2 Probisearch, C/Santiago Grisolía 2, 28760 Tres Cantos, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Espinosa-Martos
authors.affiliation: 1 Department of Nutrition, Food Science and Food Technology, Complutense University of Madrid, Av. Puerta de Hierro, s/n, 28040 Madrid, Spain.|2 Probisearch, C/Santiago Grisolía 2, 28760 Tres Cantos, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Jiménez
authors.affiliation: 1 Department of Nutrition, Food Science and Food Technology, Complutense University of Madrid, Av. Puerta de Hierro, s/n, 28040 Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: de Andrés
authors.affiliation: 3 Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.
authors.forename: L M
authors.initials: LM
authors.lastname: Rodríguez-Alcalá
authors.affiliation: 4 Department of Biochemistry and Molecular Biology, University of Valencia, Av. Doctor Moliner 50, 46100 Burjassot, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Tavárez
authors.affiliation: 2 Probisearch, C/Santiago Grisolía 2, 28760 Tres Cantos, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Manzano
authors.affiliation: 1 Department of Nutrition, Food Science and Food Technology, Complutense University of Madrid, Av. Puerta de Hierro, s/n, 28040 Madrid, Spain.|2 Probisearch, C/Santiago Grisolía 2, 28760 Tres Cantos, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Fernández
authors.affiliation: 4 Department of Biochemistry and Molecular Biology, University of Valencia, Av. Doctor Moliner 50, 46100 Burjassot, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Alonso
authors.affiliation: 3 Institute of Food Science Research (CIAL), CSIC-UAM, C/Nicolás Cabrera 9, Campus de Cantoblanco, 28049 Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Fontecha
authors.affiliation: 1 Department of Nutrition, Food Science and Food Technology, Complutense University of Madrid, Av. Puerta de Hierro, s/n, 28040 Madrid, Spain.|2 Probisearch, C/Santiago Grisolía 2, 28760 Tres Cantos, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez
mesh_terms: D000284:Administration, Oral; D015415:Biomarkers / Q000032:analysis*; D001774:Blood Chemical Analysis; D005260:Female; D006801:Humans; D000069996:Ligilactobacillus salivarius; D008413:Mastitis / Q000628:therapy*; D008895:Milk, Human / Q000737:chemistry*; D019936:Probiotics / Q000008:administration & dosage*; D016896:Treatment Outcome; D016482:Urinalysis
chemical_list: D015415:Biomarkers
keywords: Lactobacillus salivarius; blood; mastitis; milk; probiotics