title: Carbon dioxide sequestration by chemolithotrophic oleaginous bacteria for production and optimization of polyhydroxyalkanoate.
abstract: The present work involved screening of a previously reported carbon concentrating oleaginous bacterial strain Serratia sp. ISTD04 for production of PHA and optimization of process parameters for enhanced PHA and biomass generation. The selected bacterial strain was screened for PHA production based on Nile red staining followed by visualization under fluorescence microscope. Spectrofluorometric measurement of Nile red fluorescence of the bacterial culture was also done. Confirmatory analysis of PHA accumulation by GC-MS revealed the presence of 3-hydroxyvalerate. Detection of characteristic peaks in the FT-IR spectrum further confirmed the production of PHA by the bacterium. Response Surface Methodology was used for optimization of pH and carbon sources' concentrations for higher PHA production. There was almost a 2 fold increase in the production of PHA following optimization as compared to un-optimized condition. The study thus establishes the production of PHA by Serratia sp. ISTD04.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: asmitagupta5@gmail.com.
authors.forename: Asmita
authors.initials: A
authors.lastname: Gupta
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: isthakur@hotmail.com.
authors.forename: Indu Shekhar
authors.initials: IS
authors.lastname: Thakur
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide; D057965:Carbon Sequestration; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism; D010078:Oxazines / Q000737:chemistry; D010421:Pentanoic Acids / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012705:Serratia / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010078:Oxazines; D010421:Pentanoic Acids; D054813:Polyhydroxyalkanoates; C013056:beta-hydroxyvaleric acid; D002245:Carbon Dioxide; D002244:Carbon; D005947:Glucose; C044808:nile red
keywords: 3-Hydroxyvalerate; CO(2) sequestration; Polyhydroxyalkanoate; Response Surface Methodology; Serratia sp.