title: [Relationship among the Oxygen Concentration, Reactive Oxygen Species and the Biological Characteristics of Mouse Bone Marrow Hematopoietic Stem Cells].
abstract: OBJECTIVE
  To investigate the effects of oxygen concentration and reactive oxygen species (ROS) on the biological characteristics of hematopoietic stem cells (HSC) and to analyzed the relationship among the oxygen concentration, ROS and the biological characteristics of mouse HSC through simulation of oxygen environment experienced by PB HSC during transplantation.
  
  
  METHODS
  The detection of reactive oxygen species (ROS), in vitro amplification, directional differentiation (BFU-E, CFU-GM, CFU-Mix), homing of adhesion molecules (CXCR4, CD44, VLA4, VLA5, P-selectin), migration rate, CFU-S of NOD/SCID mice irradiated with sublethal dose were performed to study the effect of oxgen concentration and reactive oxygen species on the biological characteristics of mouse BM-HSC and the relationship among them.
  
  
  RESULTS
  The oxygen concentrations lower than normal oxygen concentration (especially hypoxic oxygen environment) could reduce ROS level and amplify more Lin(-) c-kit(+) Sca-1(+) BM HSC, which was more helpful to the growth of various colonies (BFU-E, CFU-GM, CFU-Mix) and to maintain the migratory ability of HSC, thus promoting CFU-S growth significantly after the transplantation of HSC in NOD/SCID mice irradiated by a sublethal dose. BM HSC exposed to oxygen environments of normal, inconstant oxygen level and strenuously thanging of oxygen concentration could result in higher level of ROS, at the same time, the above-mentioned features and functional indicators were relatively lower.
  
  
  CONCLUSIONS
  The ROS levels of BM HSC in PB HSCT are closely related to the concentrations and stability of oxygen surrounding the cells. High oxygen concentration results in an high level of ROS, which is not helpful to maintain the biological characteristics of BM HSC. Before transplantation and in vitro amplification, the application of antioxidancs and constant oxygen level environments may be beneficial for transplantation of BMMSC.
authors.affiliation: Department of Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Si-Hua
authors.initials: SH
authors.lastname: Ren
authors.affiliation: Department of Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: He
authors.affiliation: Department of Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Yi-Ran
authors.initials: YR
authors.lastname: Ma
authors.affiliation: Department of Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
authors.forename: Jing-Chun
authors.initials: JC
authors.lastname: Jin
authors.affiliation: Department of Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. E-mail: kangdan35063314@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Kang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media / Q000737:chemistry; D015672:Erythroid Precursor Cells / Q000166:cytology; D055014:Granulocyte-Macrophage Progenitor Cells / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010100:Oxygen / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D003470:Culture Media; D017382:Reactive Oxygen Species; D010100:Oxygen