title: Bioelectric and Morphological Response of Liquid-Covered Human Airway Epithelial Calu-3 Cell Monolayer to Periodic Deposition of Colloidal 3-Mercaptopropionic-Acid Coated CdSe-CdS/ZnS Core-Multishell Quantum Dots.
abstract: Lung epithelial cells are extensively exposed to nanoparticles present in the modern urban environment. Nanoparticles, including colloidal quantum dots (QDs), are also considered to be potentially useful carriers for the delivery of drugs into the body. It is therefore important to understand the ways of distribution and the effects of the various types of nanoparticles in the lung epithelium. We use a model system of liquid-covered human airway epithelial Calu-3 cell cultures to study the immediate and long-term effects of repeated deposition of colloidal 3-mercaptopropionic-acid coated CdSe-CdS/ZnS core-multishell QDs on the lung epithelial cell surface. By live confocal microscope imaging and by QD fluorescence measurements we show that the QD permeation through the mature epithelial monolayers is very limited. At the time of QD deposition, the transepithelial electrical resistance (TEER) of the epithelial monolayers transiently decreased, with the decrement being proportional to the QD dose. Repeated QD deposition, once every six days for two months, lead to accumulation of only small amounts of the QDs in the cell monolayer. However, it did not induce any noticeable changes in the long-term TEER and the molecular morphology of the cells. The colloidal 3-mercaptopropionic-acid coated CdSe-CdS/ZnS core-multishell QDs could therefore be potentially used for the delivery of drugs intended for the surface of the lung epithelia during limited treatment periods.
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Aizat
authors.initials: A
authors.lastname: Turdalieva
authors.affiliation: AstraZeneca R&D, SE-431 83 Mölndal, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Solandt
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Nestan
authors.initials: N
authors.lastname: Shambetova
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Advanced Light Microscopy facility, Science for Life Laboratory, Solna, Sweden.
authors.forename: Hans
authors.initials: H
authors.lastname: Blom
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Hjalmar
authors.initials: H
authors.lastname: Brismar
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Marina
authors.initials: M
authors.lastname: Zelenina
authors.affiliation: Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fu
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000494:pharmacology*; D019187:Cadmium Compounds / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques; D002460:Cell Line; D004337:Drug Carriers / Q000494:pharmacology*; D017097:Electric Impedance; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D053758:Nanoparticles; D045663:Quantum Dots / Q000737:chemistry*; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects*; D018036:Selenium Compounds / Q000737:chemistry / Q000494:pharmacology; D013440:Sulfides / Q000737:chemistry / Q000494:pharmacology; D017967:Zinc Compounds / Q000737:chemistry / Q000494:pharmacology
chemical_list: D019187:Cadmium Compounds; D004337:Drug Carriers; D004791:Enzyme Inhibitors; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C034939:cadmium sulfide; C058667:cadmium selenide; D015097:3-Mercaptopropionic Acid; C031238:zinc sulfide