title: Succinyl-proteome profiling of a high taxol containing hybrid Taxus species (Taxus × media) revealed involvement of succinylation in multiple metabolic pathways.
abstract: Protein lysine succinylation, a ubiquitous protein post-translational modification among eukaryotic and prokaryotic cells, represents a vital regulator of various metabolic processes. However, little is known about its functions and cellular distribution in Taxus × media, which is a hybrid Taxus species containing a high content of taxol. In this study, LC-MS/MS was used to identify peptides enriched by immune-purification with high-efficiency succinyl-lysine antibody. A total of 193 succinylated proteins and 325 succinylation sites were identified. The bioinformatics analysis indicated that these succinylated proteins were involved in a wide range of cellular functions from metabolism to protein binding and showed diverse subcellular localizations. Furthermore, our findings suggested that lysine succinylation in Taxus × media involved a diverse array of metabolic processes and protein-protein interactions. Many enzymes involved in multiple metabolic pathways, such as glycolysis, pyruvate metabolism, the tricarboxylic acid cycle and carbon fixation, were identified as substrates for lysine succinylation, suggesting the presence of a common mechanism underlying the participation of succinylation in metabolic regulation. These results provide the first comprehensive view of the succinylome of Taxus × media and may catalyze future biological investigation of succinylation.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Chenjia
authors.initials: C
authors.lastname: Shen
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xue
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27607.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Meng
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Huizhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis*; D015153:Blotting, Western; D057486:Carbon Cycle / Q000235:genetics; D002853:Chromatography, Liquid; D002952:Citric Acid Cycle / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006019:Glycolysis / Q000235:genetics; D008239:Lysine / Q000378:metabolism; D058977:Molecular Sequence Annotation; D017239:Paclitaxel / Q000096:biosynthesis*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D020543:Proteome; D019289:Pyruvic Acid / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D020946:Taxus / Q000235:genetics / Q000378:metabolism*
chemical_list: D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic; D010940:Plant Proteins; D020543:Proteome; D019289:Pyruvic Acid; D019802:Succinic Acid; D008239:Lysine; D017239:Paclitaxel