title: The role of basophil activation test in allergic bronchopulmonary aspergillosis and Aspergillus fumigatus sensitization in cystic fibrosis patients.
abstract: Allergic bronchopulmonary aspergillosis (ABPA) and Aspergillus fumigatus sensitization (AFS) are quite often observed in cystic fibrosis (CF) patients. The aim of this study was to evaluate the use of basophil activation test (BAT) in these manifestations of hypersensitivity reactions.
  
  
  
  BAT (CD63 and CD203c) was performed for 56 CF patients (17 ABPA, 24 AFS and 15 non-AFS). A receiver operating characteristic (ROC) curve analysis and a survival analysis were performed.
  
  
  
  Both markers significantly contributed in ABPA diagnosis (P value<0.001 for both). Twelve AFS patients fulfilled the criteria for ABPA diagnosis during the follow-up period. The median time to ABPA diagnosis was 9months for patients whose values were over the best cutoffs (P value<0.001 for both).
  
  
  
  BAT could be considered as an additional criterion for ABPA diagnosis in CF as well as a potential marker for the monitoring of patients with AFS.
authors.affiliation: Department of Cystic Fibrosis, "Aghia Sophia" Children's Hospital, Thivon & Papadiamantopoulou str., GR11527, Athens, Greece. Electronic address: katelarianna@gmail.com.
authors.forename: Anna
authors.initials: A
authors.lastname: Katelari
authors.affiliation: Department of Immunology & Histocompatibility, "Aghia Sophia" Children's Hospital, Thivon & Papadiamantopoulou str., GR11527, Athens, Greece.
authors.forename: Marianna
authors.initials: M
authors.lastname: Tzanoudaki
authors.affiliation: Department of Cystic Fibrosis, "Aghia Sophia" Children's Hospital, Thivon & Papadiamantopoulou str., GR11527, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Noni
authors.affiliation: Department of Immunology & Histocompatibility, "Aghia Sophia" Children's Hospital, Thivon & Papadiamantopoulou str., GR11527, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Kanariou
authors.affiliation: First Department of Pediatrics, Medical School, National and Kapodistian University of Athens, "Aghia Sophia" Children's Hospital, Thivon & Papadiamantopoulou str., GR11527, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Theodoridou
authors.affiliation: Department of Medical Genetics, Medical School, Choremeion Research Center, National and Kapodistrian University of Athens, Thivon & Livadias str., GR11526, Athens, Greece.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Kanavakis
authors.affiliation: Department of Cystic Fibrosis, "Aghia Sophia" Children's Hospital, Thivon & Papadiamantopoulou str., GR11527, Athens, Greece.
authors.forename: Stavros-Eleftherios
authors.initials: SE
authors.lastname: Doudounakis
authors.affiliation: First Department of Pediatrics, Medical School, National and Kapodistian University of Athens, "Aghia Sophia" Children's Hospital, Thivon & Papadiamantopoulou str., GR11527, Athens, Greece.
authors.forename: Christina
authors.initials: C
authors.lastname: Kanaka-Gantenbein
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000032:analysis; D000946:Antigens, Fungal / Q000032:analysis; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000150:complications / Q000175:diagnosis / Q000276:immunology; D001232:Aspergillus fumigatus / Q000276:immunology / Q000302:isolation & purification; D001491:Basophils / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications / Q000175:diagnosis / Q000276:immunology; D003584:Cytological Techniques / Q000379:methods; D005260:Female; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D008297:Male; D012372:ROC Curve; D015203:Reproducibility of Results; D013183:Sputum / Q000276:immunology; D060149:Tetraspanin 30 / Q000032:analysis*
chemical_list: D000485:Allergens; D000946:Antigens, Fungal; D015415:Biomarkers; D060149:Tetraspanin 30
keywords: Allergic bronchopulmonary aspergillosis; Aspergillus fumigatus; Aspergillus fumigatus sensitization; Basophil activation test; Cystic fibrosis