title: Protective effects of Se-containing protein hydrolysates from Se-enriched rice against Pb(2+)-induced cytotoxicity in PC12 and RAW264.7 cells.
abstract: The protective capacity of Se-containing protein hydrolysates with molecular weight below 1kDa (SPHs-3), against Pb(2+)-induced damage in PC12 and RAW264.7 cells, was investigated in this study. The cell viability, levels of reactive oxygen species (ROS), lactate dehydrogenase (LDH), superoxide dismutase (SOD), nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) in cell were analyzed. Results showed that 100μg/ml of SPHs-3 pretreatment could significantly increase cell viability by 24.9% and 23.0% in Pb(2+)-treated PC12 and RAW264.7 cells, respectively (P<0.01). The levels of ROS, NO, LDH and MDA were reduced by 32.2%, 68.2%, 79.7% and 73.7% in 100μg/ml SPHs-3 pretreated PC12 cells, respectively (P<0.01). SPHs-3 pretreatment was also associated with increases of SOD activity and GSH content in cells. In conclusion, SPHs-3 could protect cells against Pb(2+)-induced cytotoxicity, suggesting that Se-enriched rice may be a feasible candidate to improve health standard of the Pb(2+)-pollution population.
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Pei
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Benard Muinde
authors.initials: BM
authors.lastname: Kimatu
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Hu
authors.affiliation: College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, People's Republic of China. Electronic address: weifenqiu711@163.com.
authors.forename: Weifen
authors.initials: W
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection; D005978:Glutathione / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D051379:Mice; D012275:Oryza / Q000737:chemistry*; D016716:PC12 Cells; D011492:Protein Hydrolysates / Q000494:pharmacology*; D051381:Rats; D012643:Selenium / Q000494:pharmacology*
chemical_list: D011492:Protein Hydrolysates; D007854:Lead; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D012643:Selenium
keywords: Cytotoxicity; Lead; Protein hydrolysates; Rice; Selenium