title: The crucial role of IL-22 and its receptor in thymus and activation regulated chemokine production and T-cell migration by house dust mite extract.
abstract: House dust mite (HDM) is known as one of the factors that causes atopic dermatitis (AD). Interleukin (IL)-22 and thymus and activation regulated chemokine (TARC) are related to skin inflammatory disease and highly expressed in AD lesions. However, the effects of HDM on IL-22 production in T cells and on TARC production and IL-22Rα receptor expression in keratinocytes are unknown. To identify the role of HDM in keratinocytes and T cells, we investigated IL-22Rα expression and TARC production in the human keratinocyte cell line HaCaT and IL-22 production in T cells treated with HDM extract as well as their roles in HDM-induced skin inflammation. HDM extract not only increased IL-22Rα expression and TARC production in HaCaT but also enhanced IL-22, tumor necrosis factor (TNF)-α and interferon (IFN)-γ production in T cells. The HDM extract-induced IL-22 from T cells significantly increased the production of IL-1α, IL-6 and TARC in HaCaT cells. In addition, we found that TARC produced in HDM extract-treated HaCaT induced T-cell recruitment. These results suggest that there is a direct involvement of HDM extract-induced IL-22 in TARC production and T-cell migration. Taken together, TARC production in HaCaT through the interaction between IL-22 and IL-22Rα facilitates T-cell migration. These data show one of the reasons for inflammation in the skin lesions of AD patients.
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Mirim
authors.initials: M
authors.lastname: Jang
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, Korea.
authors.forename: Hyemin
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Yejin
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jiyea
authors.initials: J
authors.lastname: Choi
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jane
authors.initials: J
authors.lastname: Jeon
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Youngil
authors.initials: Y
authors.lastname: Hwang
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.|Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, Korea.
authors.forename: Jae Seung
authors.initials: JS
authors.lastname: Kang
authors.affiliation: Department of Anatomy and Cell Biology, Laboratory of Vitamin C and Antioxidant Immunology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Wang Jae
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement; D054414:Chemokine CCL17 / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D053582:Interleukin-1alpha / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism*; D015603:Keratinocytes / Q000276:immunology* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D039981:Pyroglyphidae / Q000276:immunology*; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D018123:Receptors, Interleukin / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D054414:Chemokine CCL17; D048049:Extracellular Signal-Regulated MAP Kinases; D007371:Interferon-gamma; D053582:Interleukin-1alpha; D015850:Interleukin-6; D007378:Interleukins; D051057:Proto-Oncogene Proteins c-akt; D018336:Receptors, Aryl Hydrocarbon; D018123:Receptors, Interleukin; D050796:STAT3 Transcription Factor; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22; C111235:AHR protein, human; C106230:CCL17 protein, human; C508600:IL6 protein, human; C494086:STAT3 protein, human; C415369:interleukin-22 receptor
keywords: atopic dermatitis; house dust mite; interleukin-22; interleukin-22 receptor; thymus and activation regulated chemokine