title: Mechanism of action of a novel recombinant peptide, MP1102, against Clostridium perfringens type C.
abstract: This work is the first to report the antibacterial characteristics and antibacterial mechanisms of MP1102, which is a variant of NZ2114, against pathogenic Clostridium perfringens. MP1102 exhibited strong antimicrobial activity against C. perfringens strains CVCC 61, CVCC 1163, and CVCC 2032 at a low minimal inhibitory concentration (MIC) of 0.91 μM. MP1102 showed anti-C. perfringens activity over a wide pH range of 2.0 and 10.0, high thermal stability from 20 to 80 °C, and remarkable resistance to pepsin. The fractional inhibitory concentration index (FICI) indicated an additive or synergic effect between MP1102 and bacitracin zinc, nisin, vancomycin, virginiamycin, aureomycin, and ampicillin against C. perfringens (FICI = 0.3125-1.0). To further elucidate the antibacterial mechanism of MP1102, its effect on the C. perfringens CVCC 61 cell membrane and intracellular DNA was studied. Flow cytometry and scanning electron microscopy (SEM) indicated that MP1102 treatment resulted in the release of cellular contents by damaging the membrane. A DNA gel retardation and circular dichroism analysis demonstrated that MP1102 interacted with DNA and intercalated into the DNA base pairs. A cell cycle assay demonstrated that MP1102 affected cellular functions, such as DNA synthesis. These results suggested that MP1102 exhibited potential as a new antimicrobial agent against C. perfringens infections.
authors.affiliation: Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People's Republic of China.|Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Zong
authors.affiliation: Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People's Republic of China. tengda@caas.cn.|Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China. tengda@caas.cn.
authors.forename: Da
authors.initials: D
authors.lastname: Teng
authors.affiliation: Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People's Republic of China.|Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People's Republic of China.|Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Mao
authors.affiliation: Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People's Republic of China.|Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People's Republic of China.|Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People's Republic of China. wangjianhua@caas.cn.|Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People's Republic of China. wangjianhua@caas.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wang
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002751:Chlortetracycline / Q000494:pharmacology; D003016:Clostridium perfringens / Q000187:drug effects* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004357:Drug Synergism; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009561:Nisin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology*; D013696:Temperature; D014640:Vancomycin / Q000494:pharmacology; D014769:Virginiamycin / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D014769:Virginiamycin; D009561:Nisin; D014640:Vancomycin; D000667:Ampicillin; D015032:Zinc; D002751:Chlortetracycline
keywords: Antimicrobial characterization; Antimicrobial peptides; Clostridium perfringens; Intracellular interference; MP1102; Membrane damage