title: Evaluation of Verigene Blood Culture Test Systems for Rapid Identification of Positive Blood Cultures.
abstract: The performance of molecular tests using the Verigene Gram-Positive and Gram-Negative Blood Culture nucleic acid tests (BC-GP and BC-GN, resp.; Naosphere, Northbrook, IL, USA) was evaluated for the identification of microorganisms detected from blood cultures. Ninety-nine blood cultures containing Gram-positive bacteria and 150 containing Gram-negative bacteria were analyzed using the BC-GP and BC-GN assays, respectively. Blood cultures were performed using the Bactec blood culture system (BD Diagnostic Systems, Franklin Lakes, NJ, USA) and conventional identification and antibiotic-susceptibility tests were performed using a MicroScan system (Siemens, West Sacramento, CA, USA). When a single strain of bacteria was isolated from the blood culture, Verigene assays correctly identified 97.9% (94/96) of Gram-positive bacteria and 93.8% (137/146) of Gram-negative bacteria. Resistance genes mecA and vanA were correctly detected by the BC-GP assay, while the extended-spectrum β-lactamase CTX-M and the carbapenemase OXA resistance gene were detected from 30 cases cultures by the BC-GN assay. The BC-GP and BC-GN assays showed high agreement with conventional identification and susceptibility tests. These tests are useful for rapid identification of microorganisms and the detection of clinically important resistance genes from positive Bactec blood cultures.
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, 22 Gwanpyeong-ro 170 beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do 431-796, Republic of Korea.
authors.forename: Jae-Seok
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, 22 Gwanpyeong-ro 170 beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do 431-796, Republic of Korea.
authors.forename: Go-Eun
authors.initials: GE
authors.lastname: Kang
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, 22 Gwanpyeong-ro 170 beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do 431-796, Republic of Korea.
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, 22 Gwanpyeong-ro 170 beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do 431-796, Republic of Korea.
authors.forename: Hyun Soo
authors.identifier: 0000-0002-7026-6715
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, 22 Gwanpyeong-ro 170 beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do 431-796, Republic of Korea.
authors.forename: Wonkeun
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, 22 Gwanpyeong-ro 170 beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do 431-796, Republic of Korea.
authors.forename: Kyu Man
authors.initials: KM
authors.lastname: Lee
mesh_terms: D016470:Bacteremia / Q000097:blood* / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics; D001769:Blood / Q000382:microbiology*; D019729:Carbon-Oxygen Ligases / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; D001618:beta-Lactamases; C063912:carbapenemase; D019729:Carbon-Oxygen Ligases