title: Selank Administration Affects the Expression of Some Genes Involved in GABAergic Neurotransmission.
abstract: Clinical studies have shown the similarity of the spectrum of physiological effects of Selank and classical benzodiazepines, such as diazepam and phenazepam. These data suggest that there is a similar basis of their mechanism of action. To test this hypothesis we studied the effect of Selank and GABA on the expression of genes involved in neurotransmission. We analyzed the expression of 84 genes involved in neurotransmission (e.g., major subunit of the GABA receptor, transporters, ion channels, dopamine, and serotonin receptors) in the frontal cortex of rats 1 and 3 h after the administration of Selank or GABA (300 μg/kg) using real-time PCR method. We found significant changes in the expression of 45 genes 1 h after the administration of the compounds. Three hours after Selank or GABA administration, 22 genes changed their expression. We found positive correlation between the changes in genes expression within 1 h after administration of Selank or GABA. Our results showed that Selank caused a number of alterations in the expression of genes involved in neurotransmission. The data obtained indicate that Selank is characterized by its complex effects on nerve cells, and one of its possible molecular mechanisms is associated with allosteric modulation of the GABAergic system.
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences Moscow, Russia.
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Volkova
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences Moscow, Russia.
authors.forename: Maria
authors.initials: M
authors.lastname: Shadrina
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences Moscow, Russia.
authors.forename: Timur
authors.initials: T
authors.lastname: Kolomin
authors.affiliation: The Department of Chemistry of Physiologically Active Compounds, Institute of Molecular Genetics Russian Academy of Sciences Moscow, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Andreeva
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences Moscow, Russia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Limborska
authors.affiliation: The Department of Chemistry of Physiologically Active Compounds, Institute of Molecular Genetics Russian Academy of Sciences Moscow, Russia.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Myasoedov
authors.affiliation: The Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics Russian Academy of Sciences Moscow, Russia.
authors.forename: Petr
authors.initials: P
authors.lastname: Slominsky
keywords: GABA; Selank; gene expression; glyproline; regulatory peptide