title: Gene therapy using retrovirus vectors: vector development and biosafety at clinical trials.
abstract: Retrovirus vectors (gammaretroviral and lentiviral vectors) have been considered as promising tools to transfer therapeutic genes into patient cells because they can permanently integrate into host cellular genome. To treat monogenic, inherited diseases, retroviral vectors have been used to add correct genes into patient cells. Conventional gammaretroviral vectors achieved successful results in clinical trials: treated patients had therapeutic gene expression in target cells and had improved symptoms of diseases. However, serious side-effects of leukemia occurred, caused by retroviral insertional mutagenesis (IM). These incidences stressed the importance of monitoring vector integration sites in patient cells as well as of re-consideration on safer vectors. More recently lentiviral vectors which can deliver genes into non-dividing cells started to be used in clinical trials including neurological disorders, showing their efficacy. Vector integration site analysis revealed that lentiviruses integrate less likely to near promoter regions of oncogenes than gammaretroviruses and no adverse events have been reported in lentiviral vector-mediated gene therapy clinical trials. Therefore lentiviral vectors have promises to be applied to a wide range of common diseases in near future. For example, T cells from cancer patients were transduced to express chimeric T cell receptors recognizing their tumour cells enhancing patients' anti-cancer immunity.
authors.affiliation: MRC/UCL Centre for Medical Molecular Virology and Wohl Virion Centre, Division of infection and Immunity, University College London.
authors.forename: Knayo
authors.initials: K
authors.lastname: Doi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takeuchi
mesh_terms: D002986:Clinical Trials as Topic; D015316:Genetic Therapy / Q000009:adverse effects / Q000379:methods* / Q000639:trends*; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use*; D015894:Genome, Human; D016679:Genome, Viral; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D007938:Leukemia / Q000209:etiology; D016254:Mutagenesis, Insertional; D009369:Neoplasms / Q000628:therapy; D011948:Receptors, Antigen, T-Cell; D012190:Retroviridae / Q000235:genetics; D012449:Safety
chemical_list: D011948:Receptors, Antigen, T-Cell