title: Quantitative Detection of the Foot-And-Mouth Disease Virus Serotype O 146S Antigen for Vaccine Production Using a Double-Antibody Sandwich ELISA and Nonlinear Standard Curves.
abstract: The efficacy of an inactivated foot-and-mouth disease (FMD) vaccine is mainly dependent on the integrity of the foot-and-mouth disease virus (FMDV) particles. At present, the standard method to quantify the active component, the 146S antigen, of FMD vaccines is sucrose density gradient (SDG) analysis. However, this method is highly operator dependent and difficult to automate. In contrast, the enzyme-linked immunosorbent assay (ELISA) is a time-saving technique that provides greater simplicity and sensitivity. To establish a valid method to detect and quantify the 146S antigen of a serotype O FMD vaccine, a double-antibody sandwich (DAS) ELISA was compared with an SDG analysis. The DAS ELISA was highly correlated with the SDG method (R2 = 0.9215, P<0.01). In contrast to the SDG method, the DAS ELISA was rapid, robust, repeatable and highly sensitive, with a minimum quantification limit of 0.06 μg/mL. This method can be used to determine the effective antigen yields in inactivated vaccines and thus represents an alternative for assessing the potency of FMD vaccines in vitro. But it still needs to be prospectively validated by analyzing a new vaccine preparation and determining the proper protective dose followed by an in vivo vaccination-challenge study to confirm the ELISA findings.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Jun-Wu
authors.initials: JW
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Shi-Qi
authors.initials: SQ
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Guang-Qing
authors.initials: GQ
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: He
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Shu-yun
authors.initials: SY
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Mi
authors.initials: M
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Hu
authors.initials: H
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, The People's Republic of China.
authors.forename: Xiang-Tao
authors.initials: XT
authors.lastname: Liu
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D057230:Limit of Detection; D015164:Vaccines, Inactivated / Q000032:analysis / Q000276:immunology; D014765:Viral Vaccines / Q000032:analysis* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines