title: Global methylation status of sperm DNA in carriers of chromosome structural aberrations.
abstract: Male infertility might be clearly associated with aberrant DNA methylation patterns in human spermatozoa. An association between oxidative stress and the global methylation status of the sperm genome has also been suggested. The aim of the present study was to determine whether the global sperm DNA methylation status was affected in the spermatozoa of carriers of chromosome structural aberrations. The relationships between the 5-methylcytosine (m 5 C) levels in spermatozoa and chromatin integrity status were evaluated. The study patients comprised male carriers of chromosome structural aberrations with reproductive failure (n = 24), and the controls comprised normozoospermic sperm volunteers (n = 23). The global m 5 C level was measured using thin-layer chromatography (TLC) and immunofluorescence (IF) techniques. The sperm chromatin integrity was assessed using aniline blue (AB) staining and TUNEL assay. The mean m 5 C levels were similar between the investigated chromosome structural aberrations carriers (P) and controls (K). However, sperm chromatin integrity tests revealed significantly higher values in chromosomal rearrangement carriers than in controls (P < 0.05). Although the potential relationship between sperm chromatin integrity status and sperm DNA fragmentation and the m 5 C level juxtaposed in both analyzed groups (P vs K) was represented in a clearly opposite manner, the low chromatin integrity might be associated with the high hypomethylation status of the sperm DNA observed in carriers of chromosome structural aberrations.
authors.affiliation: Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Olszewska
authors.affiliation: Department of RNA Biology, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Miroslawa Z
authors.initials: MZ
authors.lastname: Barciszewska
authors.affiliation: Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Fraczek
authors.affiliation: Institute of Hereditary Pathology, Ukrainian Academy of Medical Sciences, Lysenko Street 31a, 79000 Lviv, Ukraine.
authors.forename: Nataliya
authors.initials: N
authors.lastname: Huleyuk
authors.affiliation: Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moskvorechie Street 1, 115478 Moscow, Russian Federation.
authors.forename: Vyacheslav B
authors.initials: VB
authors.lastname: Chernykh
authors.affiliation: Institute of Hereditary Pathology, Ukrainian Academy of Medical Sciences, Lysenko Street 31a, 79000 Lviv, Ukraine.
authors.forename: Danuta
authors.initials: D
authors.lastname: Zastavna
authors.affiliation: Department of RNA Biology, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Barciszewski
authors.affiliation: Department of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Kurpisz
mesh_terms: D044503:5-Methylcytosine / Q000378:metabolism*; D000328:Adult; D016022:Case-Control Studies; D002843:Chromatin / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D002869:Chromosome Aberrations; D053938:DNA Fragmentation; D019175:DNA Methylation; D005455:Fluorescent Antibody Technique; D015321:Gene Rearrangement; D006801:Humans; D020287:In Situ Nick-End Labeling; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D013094:Spermatozoa / Q000378:metabolism*; D014178:Translocation, Genetic
chemical_list: D002843:Chromatin; D044503:5-Methylcytosine