title: Restriction of dietary protein does not promote hepatic lipogenesis in lean or fatty pigs.
abstract: The influence of genotype (lean v. fatty) and dietary protein level (normal v. reduced) on plasma metabolites, hepatic fatty acid composition and mRNA levels of lipid-sensitive factors is reported for the first time, using the pig as an experimental model. The experiment was conducted on forty entire male pigs (twenty lean pigs of Large White×Landrace×Pietrain cross-breed and twenty fatty pigs of Alentejana purebreed) from 60 to 93 kg of live weight. Each pig genotype was divided into two subgroups, which were fed the following diets: a normal protein diet (NPD) equilibrated for lysine (17·5 % crude protein and 0·7 % lysine) and a reduced protein diet (RPD) not equilibrated for lysine (13·1 % crude protein and 0·4 % lysine). The majority of plasma metabolites were affected by genotype, with lean pigs having higher contents of lipids, whereas fatty pigs presented higher insulin, leptin and urea levels. RPD increased plasma TAG, free fatty acids and VLDL-cholesterol compared with NPD. Hepatic total lipids were higher in fatty pigs than in the lean genotype. RPD affected hepatic fatty acid composition but had a slight influence on gene expression levels in the liver. Sterol regulatory element-binding factor 1 was down-regulated by RPD, and fatty acid desaturase 1 (FADS1) and fatty acid binding protein 4 (FABP4) were affected by the interaction between genotype and diet. In pigs fed RPD, FADS1 was up-regulated in the lean genotype, whereas FABP4 increased in the fatty genotype. Although there is a genotype-specific effect of dietary protein restriction on hepatic lipid metabolism, lipogenesis is not promoted in the liver of lean or fatty pigs.
authors.affiliation: 1CIISA, Faculdade de Medicina Veterinária,Universidade de Lisboa,Avenida da Universidade Técnica,Alto da Ajuda,1300-477 Lisboa,Portugal.
authors.forename: Marta S
authors.initials: MS
authors.lastname: Madeira
authors.affiliation: 1CIISA, Faculdade de Medicina Veterinária,Universidade de Lisboa,Avenida da Universidade Técnica,Alto da Ajuda,1300-477 Lisboa,Portugal.
authors.forename: Virgínia M R
authors.initials: VM
authors.lastname: Pires
authors.affiliation: 1CIISA, Faculdade de Medicina Veterinária,Universidade de Lisboa,Avenida da Universidade Técnica,Alto da Ajuda,1300-477 Lisboa,Portugal.
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Alfaia
authors.affiliation: 1CIISA, Faculdade de Medicina Veterinária,Universidade de Lisboa,Avenida da Universidade Técnica,Alto da Ajuda,1300-477 Lisboa,Portugal.
authors.forename: Paula A
authors.initials: PA
authors.lastname: Lopes
authors.affiliation: 1CIISA, Faculdade de Medicina Veterinária,Universidade de Lisboa,Avenida da Universidade Técnica,Alto da Ajuda,1300-477 Lisboa,Portugal.
authors.forename: Susana V
authors.initials: SV
authors.lastname: Martins
authors.affiliation: 2iMed.UL, Faculdade de Farmácia,Universidade de Lisboa,Alto da Ajuda,1300-477 Lisboa,Portugal.
authors.forename: Rui M A
authors.initials: RM
authors.lastname: Pinto
authors.affiliation: 1CIISA, Faculdade de Medicina Veterinária,Universidade de Lisboa,Avenida da Universidade Técnica,Alto da Ajuda,1300-477 Lisboa,Portugal.
authors.forename: José A M
authors.identifier: 0000-0003-1032-5987
authors.initials: JA
authors.lastname: Prates
mesh_terms: D000818:Animals; D001823:Body Composition / Q000502:physiology*; D004032:Diet; D018753:Diet, Protein-Restricted; D044943:Fatty Acid Desaturases / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D015870:Gene Expression; D005838:Genotype; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000032:analysis / Q000097:blood; D050155:Lipogenesis / Q000235:genetics / Q000502:physiology*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D034421:Sus scrofa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014508:Urea / Q000097:blood
chemical_list: D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D007328:Insulin; D020738:Leptin; D008055:Lipids; D014508:Urea; D044943:Fatty Acid Desaturases
keywords: ACACA acetyl-CoA carboxylase α; FABP4 fatty acid binding protein 4; FADS1 fatty acid desaturase 1; FAME fatty acid methyl esters; FASN fatty acid synthase; Fatty acid composition; Gene expression; HOMA-IR homoeostasis model assessment using the insulin resistance index; Hepatic lipid metabolism; IMF intramuscular fat; NPD normal protein diet; PC principal component; Pig genotype; RPD reduced protein diet; Reduced protein diets; SCD stearoyl-CoA desaturase; SREBF1 sterol regulatory element-binding factor 1