title: [Protocadherin 10 (PCDH10) inhibits the proliferation, invasion and migration ability of BXPC-3 pancreatic cancer cells].
abstract: OBJECTIVE
  To explore the expression and biological function of protocadherin 10 (PCDH10) in pancreatic cancer cells.
  
  
  METHODS
  Reverse transcription PCR (RT-PCR) was used to detect the expression of PCDH10 in CAPAN-1, PANC-1, ASPC-1, BXPC-3 pancreatic cancer cells and the HPDE6-C7 normal human pancreatic ductal epithelial cells. Recombinant plasmid pcDNA3.1-PCDH10 and empty vector pcDNA3.1 were transfected into BXPC-3 pancreatic cancer cells via Lipofectamine(TM)2000. After transfection, the mRNA expression of PCDH10 was examined by RT-PCR, and the protein level was detected by Western blotting. CCK-8 and colony formation assays were used to analyze the cell proliferation. Cell apoptosis was tested by flow cytometry combined with annexin V-FITC/PI staining. Transwell(TM) and wound healing assays were performed to measure the invasion and migration ability of the cells.
  
  
  RESULTS
  Compared with the normal pancreatic ductal epithelial cells, the expression of PCDH10 was obviously lower in the CAPAN-1, PANC-1, BXPC-3 cells. RT-PCR and Western blotting revealed that PCDH10 expression significantly increased in BXPC-3 cells transfected with plasmid pcDNA3.1-PCDH10 compared with the ones with empty vector pcDNA3.1. CCK-8 and colony formation assays showed that the PCDH10-transfected cells grew more slowly than the empty vector-transfected cells. Annexin V-FITC/PI staining combined with flow cytometry proved that the apoptosis in the PCDH10-transfected cells remarkably increased compared with that in the control group. A reduction of the invasion and migration ability was found obviously in the PCDH10-transfected cells by Transwell(TM) assay. The wound healing assay also showed that the PCDH10-transfected cells spread the more slowly than the empty vector-transfected cells.
  
  
  CONCLUSIONS
  The expression of PCDH10 was down-regulated in the pancreatic cancer cells. PCDH10 over-expression could significantly induce cell apoptosis, and restrain proliferation, invasion and migration ability of BXPC-3 pancreatic cancer cells.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Qiu
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Bu
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. *Corresponding author, E-mail: jiangz1753@163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008055:Lipids; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000091363:Protocadherins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection / Q000379:methods
chemical_list: D015820:Cadherins; D008055:Lipids; C086724:Lipofectamine; C438603:PCDH10 protein, human; D000091363:Protocadherins