title: Feather hydrolysate from Streptomyces sampsonii GS 1322: A potential low cost soil amendment.
abstract: Process parameters for obtaining hydrolysate from hen feathers, i.e., initial pH (5.0-9.0) and incubation period (1-6 day), were set and studied, using Streptomyces sampsonii GS 1322 in submerged and solid state conditions. Under submerged conditions, complete hydrolysis of feathers was observed on fifth day [initial pH 8.0, 28 ± 2°C, shaking (150 rpm)] with release of soluble protein (2985 μg ml(-1)) and amino acids (2407 μg ml(-1)). Cell free hydrolysate showed hydrolysis of casein (18 mm), gelatin (26 mm), collagen (31 mm), hemoglobin (23 mm) and Tween 80 (35 mm) while 445 U keratinase activity. Total soluble protein reached 5 mg ml(-1) in solid state conditions. During Pot experimentation using barren agriculture soil the effect of feather hydrolysate on wheat crop were recorded. Significant increase (p<0.01) in wheat seed germination was observed in treated soils as compared to untreated. Treatment significantly increased plant height from 30 to 60 days and 30-90 days (p<0.001). Treated soil showed an increase in total microbial count, proteolytic activity, phosphate solubilizing bacteria and ammonifying bacteria, whereas pathogenic fungi load was reduced. S. sampsonii GS 1322 can be used for bio-processing of poultry wastes yielding feather hydrolysate rich in proteins and amino acids for development of low-cost organic amendment to accelerate wheat crop growth in barren agricultural land.
authors.affiliation: Molecular Biotechnology Laboratory, C.S.R.D., People's University, Bhopal, M.P., 462037, India. Electronic address: richa198@gmail.com.
authors.forename: Richa
authors.initials: R
authors.lastname: Jain
authors.affiliation: Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar, M.P., India.
authors.forename: Aakanchha
authors.initials: A
authors.lastname: Jain
authors.affiliation: Molecular Biotechnology Laboratory, C.S.R.D., People's University, Bhopal, M.P., 462037, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Rawat
authors.affiliation: Stem Cell Technology Laboratory, C.S.R.D., People's University, Bhopal, M.P., 462037, India.
authors.forename: Meera
authors.initials: M
authors.lastname: Nair
authors.affiliation: Department of Community Medicine, P.C.M.S. & R.C., People's University, Bhopal, M.P., 462037, India.
authors.forename: Raghvendra
authors.initials: R
authors.lastname: Gumashta
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002645:Chickens / Q000033:anatomy & histology; D000069599:Crop Production / Q000191:economics* / Q000379:methods*; D018556:Crops, Agricultural / Q000187:drug effects / Q000191:economics / Q000254:growth & development / Q000378:metabolism; D005241:Feathers / Q000737:chemistry* / Q000378:metabolism*; D005260:Female; D018525:Germination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D013302:Streptomyces / Q000378:metabolism*; D014908:Triticum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D000596:Amino Acids; D011506:Proteins; D012987:Soil; D010447:Peptide Hydrolases; C035855:keratinase
keywords: Agriculture; Bioprocessing; Optimization; Solid waste; Streptomyces sampsonii