title: Inactivation of dengue, chikungunya, and Ross River viruses in platelet concentrates after treatment with ultraviolet C light.
abstract: Arboviruses, including dengue (DENV 1-4), chikungunya (CHIKV), and Ross River (RRV), are emerging viruses that are a risk for transfusion safety globally. An approach for managing this risk is pathogen inactivation, such as the THERAFLEX UV-Platelets system. We investigated the ability of this system to inactivate the above mentioned arboviruses.
  
  
  
  DENV 1-4, CHIKV, or RRV were spiked into buffy coat (BC)-derived platelet (PLT) concentrates in additive solution and treated with the THERAFLEX UV-Platelets system at the following doses: 0.05, 0.1, 0.15, and 0.2 J/cm(2) (standard dose). Pre- and posttreatment samples were taken for each dose, and the level of viral infectivity was determined.
  
  
  
  At the standard ultraviolet C (UVC) dose (0.2 J/cm(2) ), viral inactivation of at least 4.43, 6.34, and 5.13 log or more, was observed for DENV 1-4, CHIKV, and RRV, respectively. A dose dependency in viral inactivation was observed with increasing UVC doses.
  
  
  
  Our study has shown that DENV, CHIKV, and RRV, spiked into BC-derived PLT concentrates, were inactivated by the THERAFLEX UV-Platelets system to the limit of detection of our assay, suggesting that this system could contribute to the safety of PLT concentrates with respect to these emerging arboviruses.
authors.affiliation: Research and Development, Australian Red Cross Blood Service.|School of Medicine, University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Helen M
authors.initials: HM
authors.lastname: Faddy
authors.affiliation: Research and Development, Australian Red Cross Blood Service.
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Fryk
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland.|QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
authors.forename: Natalie A
authors.initials: NA
authors.lastname: Prow
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Watterson
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Young
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland.
authors.forename: Roy A
authors.initials: RA
authors.lastname: Hall
authors.affiliation: MacoPharma International GmbH, Langen, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Tolksdorf
authors.affiliation: MacoPharma, Tourcoing, France.
authors.forename: Chryslain
authors.initials: C
authors.lastname: Sumian
authors.affiliation: German Red Cross Blood Service NSTOB, Springe, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Gravemann
authors.affiliation: German Red Cross Blood Service NSTOB, Springe, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Seltsam
authors.affiliation: Research and Development, Australian Red Cross Blood Service.
authors.forename: Denese C
authors.initials: DC
authors.lastname: Marks
mesh_terms: D001792:Blood Platelets / Q000821:virology*; D058731:Blood Safety / Q000379:methods; D002646:Chikungunya virus / Q000528:radiation effects; D003716:Dengue Virus / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D057230:Limit of Detection; D017713:Platelet Transfusion / Q000009:adverse effects / Q000592:standards*; D012328:RNA Viruses / Q000528:radiation effects*; D012398:Ross River virus / Q000528:radiation effects; D014466:Ultraviolet Rays; D038301:Virus Inactivation / Q000528:radiation effects*