title: Correlation between the Chemical and Genetic Relationships among Thymus saturejoides Genotypes Cultured under in vitro and in vivo Environments.
abstract: In this study, the in vitro and in vivo essential oil (EO) composition and genetic variability in six micropropagated genotypes of Thymus saturejoides Coss., a Mediterranean medicinal and aromatic plant, were analyzed by GC/MS and randomly amplified polymorphic DNA (RAPD). Yield and composition of the EO varied between genotypes. Cluster analysis based on RAPD data and EO grouped the six genotypes in three groups in both culture conditions, thus showing considerable intraspecific genetic and chemical variations. Applying the Mantel test, the result showed a significant correlation between the two proximity matrices RAPD and EO obtained from in vitro genotypes, whereas this correlation was not observed when using the EO obtained from the in vivo genotypes.
authors.affiliation: Phytobiotechnology Unit, National Agency of Medicinal and Aromatic Plants, PB 159, Taounate, Morocco.|University of Sidi Mohamed Ben Abdellah, Imouzzer Road, PB 2626, 30000, Fez, Morocco.
authors.forename: Aicha
authors.initials: A
authors.lastname: Nordine
authors.affiliation: ICARDA-INRA Cooperative Research Project, International Center for Agricultural Research in the Dry Areas (ICARDA), PB 6299, Rabat, Morocco.
authors.forename: Sripada M
authors.initials: SM
authors.lastname: Udupa
authors.affiliation: Biotechnology Research Unit, National Institute of Agronomic Research, PB 415, Rabat, Morocco.
authors.forename: Driss
authors.initials: D
authors.lastname: Iraqi
authors.affiliation: Department of Plant, Soil & General Agriculture, Southern Illinois University, Carbondale, IL, 62901-6899, USA.
authors.forename: Khalid
authors.initials: K
authors.lastname: Meksem
authors.affiliation: Faculty of Medicine and Pharmacy, Mohamed V University, Mohamed Belarbi El Alaoui ST, PB 6203, Rabat, Morocco.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hmamouchi
authors.affiliation: Phytobiotechnology Unit, National Agency of Medicinal and Aromatic Plants, PB 159, Taounate, Morocco.|University of Sidi Mohamed Ben Abdellah, Imouzzer Road, PB 2626, 30000, Fez, Morocco.
authors.forename: Abdelmalek
authors.initials: A
authors.lastname: ElMeskaoui
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D005838:Genotype; D066298:In Vitro Techniques; D019105:Random Amplified Polymorphic DNA Technique; D046930:Thymus Plant / Q000737:chemistry* / Q000235:genetics
chemical_list: D018744:DNA, Plant
keywords: Carvacrol; Essential oils; Randomly amplified polymorphic DNA; Thymol; Thymus saturejoides