title: Distribution and Origin of VIP-, SP-, and Phospholipase Cβ2 -Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana.
abstract: Previous studies have found a few intralingual ganglionic cells that were immunoreactive to vasoactive intestinal polypeptide (VIP) in the frog. A recent study reported a large number of such cells, and the possibility of the release of substance P (SP) from these. The aim of the present study was to investigate the distribution, origin, and colocalization of VIP- and SP- immunoreactive nerves in the tongue of the bullfrog, R. catesbeiana. In addition, the study also examined the colocalization of SP and phospholipase Cβ2 (PLCβ2 ) in the tongue and jugular ganglion. VIP immunoreactivity was seen in unipolar cells that were sparse in nerve bundles in the submucosal and muscle layers. The density of VIP-immunoreactive cells was approximately 4.8 cells/mm(3) . Their fibers terminated in the vicinity of the epithelial basal layer of the fungiform papillae. SP immunoreactivity was not seen in the VIP-immunoreactive cells, but was observed in pseudounipolar cells in the jugular ganglion. The SP fibers terminated close to the free surface, showing spindle- and button-like profiles. Transection of glossopharyngeal nerve resulted in the persistence of VIP-immunoreactive cells and the disappearance of SP-immunoreactive fibers in the tongue. SP immunoreactivity was co-expressed with PLCβ2 in both the tongue and jugular ganglia. No PLCβ2 immunoreactivity was seen in cells comprising the epithelial taste disk. These findings indicate that the origin of VIP nerve fibers are unipolar cells in the tongue, and SP and PLCβ2 fibers originate from pseudounipolar cells that may be able to release SP primarily in the jugular ganglion. Anat Rec, 299:929-942, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Oral Anatomy, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Tadokoro
authors.affiliation: Department of Oral Physiology, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ando
authors.affiliation: Department of Oral Health Science, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawahara
authors.affiliation: Department of Oral Physiology, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Naokazu
authors.initials: N
authors.lastname: Asanuma
authors.affiliation: Department of Oral Anatomy, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Okumura
authors.affiliation: Department of Oral Physiology, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kitagawa
authors.affiliation: Department of Oral Anatomy, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kondo
authors.affiliation: Department of Oral Anatomy, School of Dentistry, Matsumoto Dental University, Nagano, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yagasaki
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005724:Ganglia / Q000276:immunology* / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008297:Male; D009412:Nerve Fibers / Q000276:immunology* / Q000378:metabolism; D054799:Phospholipase C beta / Q000378:metabolism*; D011892:Rana catesbeiana; D013373:Substance P / Q000378:metabolism*; D014059:Tongue / Q000276:immunology* / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D054799:Phospholipase C beta
keywords: Frog; Ganglionic cells; Immunohistochemistry; Jugular ganglia; Tongue