title: Evaluation of genetic and phenotypic consistency of Bacillus coagulans MTCC 5856: a commercial probiotic strain.
abstract: Commercial probiotics preparation containing Bacillus coagulans have been sold in the market for several decades. Due to its high intra-species genomic diversity, it is very likely that B. coagulans strain may alter in different ways over multiple years of production. Therefore, the present study focuses to evaluate the genetic consistency and probiotic potential of B. coagulans MTCC 5856. Phenotypic and genotypic techniques including biochemical profiling, 16S rRNA sequencing, GTG 5″, BOX PCR fingerprinting, and Multi-Locus-Sequence typing (MLST) were carried out to evaluate the identity and consistency of the B. coagulans MTCC 5856. Further, in vitro probiotic potential, safety and stability at ambient temperature conditions of B. coagulans MTCC 5856 were evaluated. All the samples were identified as B. coagulans by biochemical profiling and 16S rRNA sequencing. GTG 5″, BOX PCR fingerprints and MLST studies revealed that the same strain was present over 3 years of commercial production. B. coagulans MTCC 5856 showed resistance to gastric acid, bile salt and exhibited antimicrobial activity in in-vitro studies. Additionally, B. coagulans MTCC 5856 was found to be non-mutagenic, non-cytotoxic, negative for enterotoxin genes and stable at ambient temperature (25 ± 2 °C) for 36 months. The data of the study verified that the same strain of B. coagulans MTCC 5856 was present in commercial preparation over multiple years of production.
authors.affiliation: Sami Labs Limited, 19/1, 19/2, First Main, Second Phase, Peenya Industrial Area, Bangalore, 560 058, Karnataka, India.|Sabinsa Corporation, 20 Lake Drive, East Windsor, NJ, 08520, USA.
authors.forename: Muhammed
authors.initials: M
authors.lastname: Majeed
authors.affiliation: Sabinsa Corporation, 20 Lake Drive, East Windsor, NJ, 08520, USA.
authors.forename: Kalyanam
authors.initials: K
authors.lastname: Nagabhushanam
authors.affiliation: Sami Labs Limited, 19/1, 19/2, First Main, Second Phase, Peenya Industrial Area, Bangalore, 560 058, Karnataka, India.
authors.forename: Sankaran
authors.initials: S
authors.lastname: Natarajan
authors.affiliation: Sami Labs Limited, 19/1, 19/2, First Main, Second Phase, Peenya Industrial Area, Bangalore, 560 058, Karnataka, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Sivakumar
authors.affiliation: Life Sciences, FrieslandCampina Research, Bronland 20, 6708 WH, Wageningen, The Netherlands.
authors.forename: Talitha
authors.initials: T
authors.lastname: Eshuis-de Ruiter
authors.affiliation: Life Sciences, FrieslandCampina Research, Bronland 20, 6708 WH, Wageningen, The Netherlands.
authors.forename: Janine
authors.initials: J
authors.lastname: Booij-Veurink
authors.affiliation: Life Sciences, FrieslandCampina Research, Bronland 20, 6708 WH, Wageningen, The Netherlands.
authors.forename: Ynte P
authors.initials: YP
authors.lastname: de Vries
authors.affiliation: Sami Labs Limited, 19/1, 19/2, First Main, Second Phase, Peenya Industrial Area, Bangalore, 560 058, Karnataka, India. furqan@samilabs.com.
authors.forename: Furqan
authors.initials: F
authors.lastname: Ali
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D058885:Multilocus Sequence Typing; D010641:Phenotype; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Bacillus coagulans MTCC 5856; Genotypic fingerprinting; LactoSpore®; MLST