title: Inhibition of Migration and Invasion by Tet-1 Overexpression in Human Lung Carcinoma H460 Cells.
abstract: In the present study, we found that lung cancer cell line (H460 cells) expressing Tet1 showed higher levels of adhesion, and Tet1 inhibited H460 cell proliferation. In addition, these cells showed a significantly reduced ability of collagen degradation and Smad2/3 phosphorylation compared to controls. Furthermore, vimentin was found to be highly expressed in larger metastatic cancer area. Tet1 overexpression was reduced in the epithelial marker E-cadherin. Moreover, Tet1 repressed cancer cell metastasis in nude mice. Collectively, these findings suggest that Tet1 expression plays a critical role in metastasis of lung cancer cells by suppression of invasion and epithelial-mesenchymal transition (EMT).
authors.affiliation: School of Life Science, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Buk-ku, Daegu, Republic of Korea.
authors.forename: Si Jun
authors.initials: SJ
authors.lastname: Park
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Lee
authors.forename: Hyeng-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Young Rae
authors.initials: YR
authors.lastname: Ji
authors.forename: Yong Hun
authors.initials: YH
authors.lastname: Sung
authors.forename: Kwang
authors.initials: K
authors.lastname: ShikChoi
authors.forename: Hum Dai
authors.initials: HD
authors.lastname: Park
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Myoung Ok
authors.initials: MO
authors.lastname: Kim
authors.forename: Zae Young
authors.initials: ZY
authors.lastname: Ryoo
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D051899:Smad2 Protein / Q000096:biosynthesis / Q000235:genetics; D014746:Vimentin / Q000096:biosynthesis / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D011518:Proto-Oncogene Proteins; C497782:SMAD2 protein, human; D051899:Smad2 Protein; D014746:Vimentin; D006899:Mixed Function Oxygenases; C462539:TET1 protein, human