title: [Presence of yeasts in primary endodontic infections and study of their susceptibility to disinfection with sodium hypochlorite at 2.5].
abstract: BACKGROUND
  Endodontic infections are characterized by their microbial polymorphism with the presence of pathogenic agents such as bacteria. The aim of this study was to investigate the presence of yeast in the root canals of teeth with pulp necrotic with or without apical periodontitis and to study the sensibility of these at disinfection at sodium hypochlorite to 2.5 %.
  
  
  METHODS
  Root canal samples, taken with paper points before and after disinfection with sodium hypochlorite dosed at 2.5 % on 50 single rooted teeth in 38 patients, were seeded in petri dishes containing Sabouraud Chloramphenicol (SC) (Conda Laboratories, Madrid). These stains were incubated in an incubator at 37°C for 48hours. The presence of yeast was confirmed by white colonies on the entire circumference of the tip paper. The identification of species was done by macroscopic and microscopic examinations associated with Blastese test.
  
  
  RESULTS
  The yeast colonies were isolated from 7 teeth (14 %) out of 50. All positive samples were from teeth with an open necrotic pulp (P<0.001). The only species found was Candida albicans. Immediate disinfection with sodium hypochlorite at 2.5 % did not show a complete elimination of yeasts.
  
  
  CONCLUSIONS
  The results of this study show that the canal of necrotic teeth with or without apical periodontitis may shelter yeasts even after immediate disinfection with sodium hypochlorite 2.5 %.
authors.affiliation: Département d'odontologie, université Cheikh Anta Diop, BP 5005, Dakar, Sénégal. Electronic address: juma.ndiaye@yahoo.fr.
authors.forename: D
authors.initials: D
authors.lastname: Ndiaye
authors.affiliation: Laboratoire de parasitologie et de mycologie, CHU Le Dantec, BP 16477, Dakar, Sénégal.
authors.forename: K
authors.initials: K
authors.lastname: Diongue
authors.affiliation: Département d'odontologie, université Cheikh Anta Diop, BP 5005, Dakar, Sénégal.
authors.forename: K
authors.initials: K
authors.lastname: Bane
authors.affiliation: Département d'odontologie, université Cheikh Anta Diop, BP 5005, Dakar, Sénégal.
authors.forename: S O
authors.initials: SO
authors.lastname: Niang
authors.affiliation: Laboratoire de parasitologie et de mycologie, CHU Le Dantec, BP 16477, Dakar, Sénégal.
authors.forename: D
authors.initials: D
authors.lastname: Ndiaye
authors.affiliation: Département d'odontologie, université Cheikh Anta Diop, BP 5005, Dakar, Sénégal.
authors.forename: B
authors.initials: B
authors.lastname: Touré
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002176:Candida albicans / Q000187:drug effects / Q000302:isolation & purification; D002648:Child; D003790:Dental Pulp Necrosis / Q000382:microbiology*; D004203:Disinfection / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology*; D012675:Senegal; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D007507:Therapeutic Irrigation; D015003:Yeasts / Q000187:drug effects* / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D012973:Sodium Hypochlorite
keywords: Canal irrigation; Candida albicans; Hypochlorite de sodium; Irrigation canalaire; Lésions péri-apicales; Necrotic pulp; Nécrose pulpaire; Periapical diseases; Sodium hypochlorite