title: Acute phase inflammation is characterized by rapid changes in plasma/peritoneal fluid N-glycosylation in mice.
abstract: Murine zymosan-induced peritonitis is a widely used model for studying the molecular and cellular events responsible for the initiation, persistence and/or resolution of inflammation. Among these events, it is becoming increasingly evident that changes in glycosylation of proteins, especially in the plasma and at the site of inflammation, play an important role in the inflammatory response. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)-based glycosylation profiling, we investigated the qualitative and quantitative effect of zymosan-induced peritonitis on N-glycosylation in mouse plasma and peritoneal fluid. Our results show that both N-glycomes exhibit highly similar glycosylation patterns, consisting mainly of diantennary and triantennary complex type N-glycans with high levels (>95 %) of galactosylation and sialylation (mostly NeuGc) and a medium degree of core fucosylation (30 %). Moreover, MS/MS structural analysis, assisted by linkage-specific derivatization of sialic acids, revealed the presence of O-acetylated sialic acids as well as disialylated antennae ("branching sialylation") characterized by the presence of α2-6-linked NeuGc on the GlcNAc of the NeuGcα2-3-Galβ1-3-GlcNAc terminal motif. A significant decrease of (core) fucosylation together with an increase of both α2-3-linked NeuGc and "branching sialylation" were observed in N-glycomes of mice challenged with zymosan, but not in control mice injected with PBS. Importantly, substantial changes in glycosylation were already observed 12 h after induction of peritonitis, thereby demonstrating an unexpected velocity of the biological mechanisms involved.
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands. yoann.rombouts@ipbs.fr.|Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands. yoann.rombouts@ipbs.fr.|Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France. yoann.rombouts@ipbs.fr.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Rombouts
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.|Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Hulda S
authors.initials: HS
authors.lastname: Jónasdóttir
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Agnes L
authors.initials: AL
authors.lastname: Hipgrave Ederveen
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Karli R
authors.initials: KR
authors.lastname: Reiding
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Bas C
authors.initials: BC
authors.lastname: Jansen
authors.affiliation: Faculty of Medicine, Biomedical Center, School of Health Sciences, University of Iceland, Reykjavik, Iceland.|Department of Immunology and Center for Rheumatology Research, Landspitali-The National University Hospital of Iceland, Reykjavik, Iceland.
authors.forename: Jona
authors.initials: J
authors.lastname: Freysdottir
authors.affiliation: Faculty of Medicine, Biomedical Center, School of Health Sciences, University of Iceland, Reykjavik, Iceland.
authors.forename: Ingibjörg
authors.initials: I
authors.lastname: Hardardottir
authors.affiliation: Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Andreea
authors.initials: A
authors.lastname: Ioan-Facsinay
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: Giera
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.|Division of BioAnalytical Chemistry, VU University Amsterdam, Amsterdam, The Netherlands.
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D000210:Acute-Phase Reaction / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D051379:Mice; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000097:blood / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
chemical_list: D006023:Glycoproteins; D000117:Acetylglucosamine
keywords: Mouse; N-glycosylation changes; Peritoneal fluid; Plasma; Zymosan-induced peritonitis