title: Metabolic Engineering of Escherichia coli for the Production of 3-Hydroxypropionic Acid and Malonic Acid through β-Alanine Route.
abstract: Escherichia coli was metabolically engineered to produce industrially important platform chemicals, 3-hydroxypropionic acid (3-HP) and malonic acid (MA), through the β-alanine (BA) route. First, various combinations of downstream enzymes were screened and BA pyruvate transaminase (encoded by pa0132) from P. aeruginosa was selected to generate malonic semialdehyde (MSA) from BA. This platform strain was engineered by introducing E. coli MSA reductase (encoded by ydfG) to reduce MSA to 3-HP. Replacement of native promoter of the sdhC gene with the strong trc promoter in the genome increased 3-HP production to 3.69 g/L in flask culture. Introduction of E. coli semialdehyde dehydrogenase (encoded by yneI) into the platform strain resulted in the production of MA, and additional deletion of the ydfG gene increased MA production to 0.450 g/L in flask culture. Fed-batch cultures of final engineered strains resulted in the production of 31.1 g/L 3-HP or 3.60 g/L MA from glucose.
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Song
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Je Woong
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: In Jin
authors.initials: IJ
authors.lastname: Cho
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.|BioInformatics Research Center and BioProcess Engineering Research Center, KAIST , Daejeon 34141, Republic of Korea.
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008314:Malonates / Q000378:metabolism*; D060847:Metabolic Engineering; D000074041:Microorganisms, Genetically-Modified / Q000235:genetics; D010088:Oxidoreductases / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015091:beta-Alanine / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008314:Malonates; C486355:SdhC protein, bacteria; D015091:beta-Alanine; D019344:Lactic Acid; C030290:malonic acid; C031601:hydracrylic acid; D010088:Oxidoreductases; D005947:Glucose
keywords: 3-hydroxypropionic acid; Escherichia coli; fumaric acid; malonic acid; metabolic engineering; β-alanine