title: Fabrication of Semiordered Nanopatterned Diamond-like Carbon and Titania Films for Blood Contacting Applications.
abstract: Biomaterials with the ability to interface with, but not activate, blood components are essential for a multitude of medical devices. Diamond-like carbon (DLC) and titania (TiO2) have shown promise for these applications; however, both support platelet adhesion and activation. This study explored the fabrication of nanostructured DLC and TiO2 thin film coatings using a block copolymer deposition technique that produced semiordered nanopatterns with low surface roughness (5-8 nm Rrms). These surfaces supported fibrinogen and plasma protein adsorption that predominantly adsorbed between the nanofeatures and reduced the overall surface roughness. The conformation of the adsorbed fibrinogen was altered on the nanopatterned surfaces as compared with the planar surfaces to reveal higher levels of the platelet binding region. Planar DLC and TiO2 coatings supported less platelet adhesion than nanopatterned DLC and TiO2. However, platelets on the nanopatterned DLC coatings were less spread indicating a lower level of platelet activation on the nanostructured DLC coatings compared with the planar DLC coatings. These data indicated that nanostructured DLC coatings may find application in blood contacting medical devices in the future.
authors.affiliation: Biomedical Engineering, School of AMME, University of Sydney , Sydney, New South Wales 2007, Australia.
authors.forename: Deepika
authors.initials: D
authors.lastname: Nandakumar
authors.affiliation: Material Science and Engineering, CSIRO , Lindfield, New South Wales 2070, Australia.
authors.forename: Avi
authors.initials: A
authors.lastname: Bendavid
authors.affiliation: Material Science and Engineering, CSIRO , Lindfield, New South Wales 2070, Australia.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Martin
authors.affiliation: National Institute for Nanotechnology, National Research Council , Edmonton, Alberta T5B 0N1, Canada.
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Harris
authors.affiliation: Biomedical Engineering, School of AMME, University of Sydney , Sydney, New South Wales 2007, Australia.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Ruys
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales , Sydney, New South Wales 2052, Australia.
authors.forename: Megan S
authors.initials: MS
authors.lastname: Lord
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D058612:Nanodiamonds / Q000737:chemistry*; D010973:Platelet Adhesiveness; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D058612:Nanodiamonds; C009495:titanium dioxide; D014025:Titanium
keywords: diamond-like carbon; fibrinogen; platelet adhesion; protein adsorption; titania