title: Uptake characteristics of levofloxacin for the eradication of Helicobacter pylori by GES-1 and MGC80-3 cells.
abstract: OBJECTIVE
  The aim of this study was to study the uptake of levofloxacin for the eradication of Helicobacter pylori by human gastric epithelial cell lines, GES-1 and MGC80-3.
  
  
  METHODS
  High performance liquid chromatography (HPLC) and coomassie brilliant blue staining, among other methods, were used to study the uptake of levofloxacin in GES-1 and MGC80-3 cells. The effect of time, concentration, temperature, pH, cyclosporine A, verapamil, and cimetidine on uptake was analyzed.
  
  
  RESULTS
  The uptake of levofloxacin by GES-1 and MGC80-3 cells reached a steady state after 15 minutes of incubation, and was enhanced by increasing the extracellular levofloxacin concentration, although not in a linear manner. A maximum uptake was observed at 37 °C and pH 7.4. Cyclosporin A and verapamil enhanced uptake in GES-1 cells by 2.07%-13.23% (p > 0.05), and 17.5%-35.3% in MGC80-3 cells (p < 0.05). The uptake of levofloxacin was not affected by cimetidine.
  
  
  CONCLUSIONS
  P-glycoprotein mediates levofloxacin uptake in MGC80-3 cell. Further, P-glycoprotein may be involved in levofloxacin uptake in GES-1 cells. However, organic cation transporters were not involved in levofloxacin uptake in MGC80-3 and GES-1 cells.
authors.affiliation: Department of Scientific Research, the First Affiliated Hospital of Anhui Medical University, Hefei, China. leizhangdoc@163.com.
authors.forename: Y-M
authors.initials: YM
authors.lastname: Hu
authors.forename: X-P
authors.initials: XP
authors.lastname: Hu
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: S-S
authors.initials: SS
authors.lastname: Zhang
authors.forename: J-M
authors.initials: JM
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D064704:Levofloxacin / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D064704:Levofloxacin