title: Kluyveromyces lactis: An emerging tool in biotechnology.
abstract: Kluyveromyces lactis has emerged as one of the most important yeast species for research and industrial biotechnology. This Crabtree-negative species is suitable for the production of metabolites and heterologous proteins, and its ability to achieve high levels of protein secretion makes it an attractive alternative for industrial protein production. Since 1991, almost 100 recombinant proteins have been expressed in K. lactis, 20% of which have been produced in the last 2 years. This review provides an overview of the genetic modifications used to accomplish heterologous gene expression in K. lactis, as well as fermentation techniques, and recent examples of industrial proteins produced in this species.
authors.affiliation: Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, Germany; Faculty of Biology and Chemistry, Justus Liebig University Giessen, Giessen, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Giessen, Germany.
authors.forename: Sebastian C
authors.initials: SC
authors.lastname: Spohner
authors.affiliation: Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, Germany.
authors.forename: Vivienne
authors.initials: V
authors.lastname: Schaum
authors.affiliation: Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, Germany.
authors.forename: Hendrich
authors.initials: H
authors.lastname: Quitmann
authors.affiliation: Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Giessen, Germany; Faculty of Biology and Chemistry, Justus Liebig University Giessen, Giessen, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Giessen, Germany; Department of Chemical Engineering, Kansas State University, Manhattan, USA. Electronic address: peter.czermak@kmub.thm.de.
authors.forename: Peter
authors.initials: P
authors.lastname: Czermak
mesh_terms: D064113:CRISPR-Cas Systems; D005818:Genetic Engineering / Q000379:methods*; D007716:Kluyveromyces; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins
keywords: CRISPR/Cas9; Kluyveromyces lactis; Model organism; Protein expression; Strain design