title: A prebiotic effect of Ecklonia cava on the growth and mortality of olive flounder infected with pathogenic bacteria.
abstract: Olive flounder (Paralichthys olivaceus), also known as the Japanese flounder in Japan, is one of the most important commercial marine finfish species cultured in Korea and Japan. The purpose of this study was to evaluate how a species of brown algae (Ecklonia cava, E. cava) affects the growth rate of olive flounder and its immune response to pathogenic bacteria. First, the experimental fish were divided into four groups: the control group was fed the diet containing only 1.0% Lactobacillus plantarum (L. plantarum), group I was fed 1.0% L. plantarum and 1.0% E. cava (EC), group II was fed 1.0% L. plantarum and 0.1% ethanol extract of EC (EE), and group III was fed 1.0% L. plantarum and 0.5% EE. The diets fed to the fish twice a day for 16 weeks. The results indicated that supplementation with 1.0% EC and 0.1% EE improved the growth and body weight of olive flounder, and decreased its mortality. This diet, however, did not significantly affect the biochemical profiles of the experimental flounder. The supplementation of 1.0% EC also enhanced the innate immune response of the fish, as evidenced by the high respiratory burst, and increased serum lysozyme and myeloperoxidase activity. The addition of 1.0% EC and either 0.1% or 0.5% EE also decreased the accumulative mortality of olive flounder infected by pathogenic bacteria (Edwardsiella tarda, Streptococcus iniae, and Vibrio harveyi). Overall, these results suggest that E. cava can act as a prebiotic by improving the innate immune response in fish infected with pathogenic bacteria as increased the growth of the probiotic.
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: WonWoo
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Marine Bio-food Science, College of Fisheries and Ocean Sciences, Chonnam National University, 500-749, Republic of Korea.
authors.forename: Ginnae
authors.initials: G
authors.lastname: Ahn
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Oh
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Nalae
authors.initials: N
authors.lastname: Kang
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Eun A
authors.initials: EA
authors.lastname: Kim
authors.affiliation: Marine Bio Research Team, Korea Basic Science Institute (KBSI), 690-140, Republic of Korea.
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Joon Bum
authors.initials: JB
authors.lastname: Jeong
authors.affiliation: Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D001835:Body Weight; D020609:Edwardsiella tarda; D004756:Enterobacteriaceae Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary; D005393:Fish Diseases / Q000473:pathology / Q000517:prevention & control; D005432:Flounder / Q000502:physiology*; D048191:Lactiplantibacillus plantarum; D009113:Muramidase / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D000459:Phaeophyceae; D056692:Prebiotics; D016897:Respiratory Burst; D013290:Streptococcal Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary; D000070058:Streptococcus iniae; D014733:Vibrio; D014735:Vibrio Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary
chemical_list: D056692:Prebiotics; D009195:Peroxidase; D009113:Muramidase
keywords: Ecklonia cava; Innate immune response; Lactobacillus plantarum; Olive flounder; Prebiotic