title: A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library.
abstract: One of the major challenges in the design of biosensors for cancer diagnosis is to introduce a low-cost and selective probe that can recognize cancer cells. In this paper, we combined the phage display technology and electrochemical impedance spectroscopy (EIS) to develop a label-free cytosensor for the detection of cancer cells, without complicated purification of recognition elements. Fabrication steps of the cytosensing interface were monitored by EIS. Due to the high specificity of the displayed octapeptides and avidity effect of their multicopy display on the phage scaffold, good biocompatibility of recombinant phage, the fibrous nanostructure of phage, and the inherent merits of EIS technology, the proposed cytosensor demonstrated a wide linear range (2.0 × 10(2) - 2.0 × 10(8) cells mL(-1)), a low limit of detection (79 cells mL(-1), S/N = 3), high specificity, good inter-and intra-assay reproducibility and satisfactory storage stability. This novel cytosensor designing strategy will open a new prospect for rapid and label-free electrochemical platform for tumor diagnosis.
authors.affiliation: Institute for Biosensing &In-Vitro Diagnostics, and College of Medicine, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China.|Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, 266101, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, 266101, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, 269 Greene Hall, Auburn, Alabama 36849-5519, United States.
authors.forename: Valery A
authors.initials: VA
authors.lastname: Petrenko
authors.affiliation: Institute for Biosensing &In-Vitro Diagnostics, and College of Medicine, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China.|Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao, 266101, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods*; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D019151:Peptide Library; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D019151:Peptide Library; D014764:Viral Proteins