title: Cross-talk between Arg methylation and Ser phosphorylation modulates apoptosis signal-regulating kinase 1 activation in endothelial cells.
abstract: We describe a novel functional interaction between ASK1 and PRMT5. We show that PRMT5 interacts with and methylates ASK1 at arginine residue 89 and thereby negatively regulates its activity by promoting the interaction between ASK1 and Akt and thus phosphorylating ASK1 at serine residue 83. Furthermore, the association between ASK1 and Akt is enhanced by VEGF stimulation, and PRMT5 is required for this association. Moreover, PRMT5-mediated ASK1 methylation impaired the H2O2-induced activity of ASK1, and this inhibitory effect of PRMT5 was abolished by replacement of arginine 89 with Trp or depletion of PRMT5 expression by RNA interference. Together the results demonstrate cross-talk between arginine methylation and serine phosphorylation in ASK1.
authors.affiliation: Center for Identification of Chinese Herbal Medicines, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China Tianjin Institute of Hygiene and Environmental Medicine, Tianjin 300050, China Shuwei7866@126.com mjh1962@vip.163.com chenmingprotein@163.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Center for Identification of Chinese Herbal Medicines, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Qu
authors.affiliation: Tianjin Institute of Hygiene and Environmental Medicine, Tianjin 300050, China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Cell Biology and Genetics, Guangxi Medical University, Nanning 530021, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Cell Biology and Genetics, Guangxi Medical University, Nanning 530021, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Cell Biology and Genetics, Guangxi Medical University, Nanning 530021, China.
authors.forename: Fuji
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Hematology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Specific Diagnosis, General Hospital of Airforce, Beijing 100142, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Tian
authors.affiliation: Center for Identification of Chinese Herbal Medicines, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China Shuwei7866@126.com mjh1962@vip.163.com chenmingprotein@163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Miao
authors.affiliation: Department of Cell Biology and Genetics, Guangxi Medical University, Nanning 530021, China Shuwei7866@126.com mjh1962@vip.163.com chenmingprotein@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Shu
mesh_terms: D001120:Arginine / Q000378:metabolism*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D048848:MAP Kinase Kinase Kinase 5 / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012694:Serine / Q000378:metabolism*; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
chemical_list: D042461:Vascular Endothelial Growth Factor A; D012694:Serine; D001120:Arginine; D006861:Hydrogen Peroxide; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases; D051057:Proto-Oncogene Proteins c-akt; D048848:MAP Kinase Kinase Kinase 5; C482858:MAP3K5 protein, human