title: hnRNP A1-mediated translational regulation of the G quadruplex-containing RON receptor tyrosine kinase mRNA linked to tumor progression.
abstract: The expression and role of RNA binding proteins (RBPs) controlling mRNA translation during tumor progression remains largely uncharacterized. Analysis by immunohistochemistry of the expression of hnRNP A1, hnRNPH, RBM9/FOX2, SRSF1/ASF/SF2, SRSF2/SC35, SRSF3/SRp20, SRSF7/9G8 in breast tumors shows that the expression of hnRNP A1, but not the other tested RBPs, is associated with metastatic relapse. Strikingly, hnRNP A1, a nuclear splicing regulator, is also present in the cytoplasm of tumor cells of a subset of patients displaying exceedingly worse prognosis. Expression of a cytoplasmic mutant of hnRNP A1 leads to increased translation of the mRNA encoding the tyrosine kinase receptor RON/MTS1R, known for its function in tumor dissemination, and increases cell migration in vitro. hnRNP A1 directly binds to the 5' untranslated region of the RON mRNA and activates its translation through G-quadruplex RNA secondary structures. The correlation between hnRNP A1 and RON tumoral expression suggests that these findings hold clinical relevance.
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Cammas
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.|Institut Claudius Regaud, Toulouse, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Lacroix-Triki
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.|Institut Claudius Regaud, Toulouse, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Pierredon
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Le Bras
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Iacovoni
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR 176, Orsay, France.|Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, France.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Teulade-Fichou
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.|Institut Claudius Regaud, Toulouse, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Favre
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.|Institut Claudius Regaud, Toulouse, France.
authors.forename: Henri
authors.initials: H
authors.lastname: Roché
authors.affiliation: Institut Claudius Regaud, Toulouse, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Filleron
authors.affiliation: INSERM UMR 1037, Centre de Recherches en Cancérologie de Toulouse, Toulouse, France.|Université Toulouse III Paul Sabatier, Toulouse, France.
authors.forename: Stefania
authors.initials: S
authors.lastname: Millevoi
authors.affiliation: Université Paris Sud, Université Paris-Saclay, CNRS UMR 176, Orsay, France.|Université Paris Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay, France.|Equipe Labellisée Ligue Contre le Cancer, Paris, France.
authors.forename: Stéphan
authors.initials: S
authors.lastname: Vagner
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053208:Kaplan-Meier Estimate; D014176:Protein Biosynthesis / Q000502:physiology; D012333:RNA, Messenger; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D012333:RNA, Messenger; C000619656:hnRNPA1 protein, human; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases
keywords: RNA-binding protein; RON; breast cancer; metastasis; translation