title: Identification and quantitation of lipid C=C location isomers: A shotgun lipidomics approach enabled by photochemical reaction.
abstract: The field of lipidomics has been significantly advanced by mass spectrometric analysis. The distinction and quantitation of the unsaturated lipid isomers, however, remain a long-standing challenge. In this study, we have developed an analytical tool for both identification and quantitation of lipid C=C location isomers from complex mixtures using online Paternò-Büchi reaction coupled with tandem mass spectrometry (MS/MS). The potential of this method has been demonstrated with an implementation into shotgun lipid analysis of animal tissues. Among 96 of the unsaturated fatty acids and glycerophospholipids identified from rat brain tissue, 50% of them were found as mixtures of C=C location isomers; for the first time, to our knowledge, the quantitative information of lipid C=C isomers from a broad range of classes was obtained. This method also enabled facile cross-tissue examinations, which revealed significant changes in C=C location isomer compositions of a series of fatty acids and glycerophospholipid (GP) species between the normal and cancerous tissues.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907;
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907;
authors.forename: Leelyn
authors.initials: L
authors.lastname: Chong
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907; Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907;
authors.forename: Ran
authors.initials: R
authors.lastname: Tian
authors.affiliation: Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907; Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907;
authors.forename: Riyi
authors.initials: R
authors.lastname: Shi
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX 77030; Department of Cell and Developmental Biology, Weill Cornell Medical College of Cornell University, New York, NY 10065.
authors.forename: Tony Y
authors.initials: TY
authors.lastname: Hu
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907; ouyang@purdue.edu yxia@purdue.edu.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ouyang
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907; ouyang@purdue.edu yxia@purdue.edu.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000737:chemistry; D020404:Glycerophospholipids / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008321:Mammary Glands, Animal / Q000737:chemistry; D015674:Mammary Neoplasms, Animal / Q000737:chemistry; D051379:Mice; D008956:Models, Chemical; D015394:Molecular Structure; D055668:Photochemical Processes; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005231:Fatty Acids, Unsaturated; D020404:Glycerophospholipids; D008055:Lipids
keywords: Paternò–Büchi reaction; cancerous tissue analysis; glycerophospholipids; lipid biomarkers; photochemical reaction