title: Lanthanide-based luminescence biolabelling.
abstract: Luminescent lanthanide complexes display unrivalled spectroscopic properties, which place them in a special category in the luminescent toolbox. Their long-lived line-like emission spectra are the cornerstones of numerous analytical applications ranging from ultrasensitive homogeneous fluoroimmunoassays to the study of molecular interactions in living cells with multiplexed microscopy. However, achieving such minor miracles is a result of years of synthetic efforts and spectroscopic studies to understand and gather all the necessary requirements for the labels to be efficient. This feature article intends to survey these criteria and to discuss some of the most important examples reported in the literature, before explaining in detail some of the applications of luminescent lanthanide labels to bioanalysis and luminescence microscopy. Finally, the emphasis will be put on some recent applications that hold great potential for future biosensing.
authors.affiliation: Laboratoire d'Ingénierie Moléculaire Appliquée à l'Analyse, IPHC, UMR 7178 CNRS, Université de Strasbourg, ECPM, Bât R1N0, 25 rue Becquerel, 67087 Strasbourg Cedex, France. l.charbonn@unistra.fr.
authors.forename: Mohamadou
authors.initials: M
authors.lastname: Sy
authors.forename: Aline
authors.initials: A
authors.lastname: Nonat
authors.forename: Niko
authors.initials: N
authors.lastname: Hildebrandt
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Charbonnière
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D006801:Humans; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods; D008853:Microscopy / Q000379:methods; D008958:Models, Molecular; D061848:Optical Imaging / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
chemical_list: D028581:Lanthanoid Series Elements; D049408:Luminescent Agents