title: The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins.
abstract: The fungal vacuole is an organelle, which adopts pleiotropic morphologies and functions. In aging and starving hyphae it is the compartment of degradation and recycling of cellular constituents. Here we identified TSP3, one of three tetraspanins present in the filamentous ascomycete fungus Neurospora crassa, as a vacuolar membrane protein. The protein is detected only in aging and starving cultures and under other conditions, which induce autophagy, such as vegetative incompatibility or the presence of the macrolide antibiotic rapamycin. Mutant analysis revealed that TSP3 is dispensable for growth and development of the fungus under laboratory conditions. Together these findings indicate that tsp3 shares characteristics with idi (induced during incompatibility) genes and might promote vacuolar functions related to autophagy.
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Heine
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Petereit
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany.
authors.forename: Marcel R
authors.initials: MR
authors.lastname: Schumann
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany.
authors.forename: Diana
authors.initials: D
authors.lastname: Patzelt
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany.
authors.forename: Leila
authors.initials: L
authors.lastname: Rachid
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Brandt
authors.affiliation: Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstraße 8, 37077, Göttingen, Germany.
authors.forename: Antonia
authors.initials: A
authors.lastname: Werner
authors.affiliation: Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstraße 8, 37077, Göttingen, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Pöggeler
authors.affiliation: Institut für Genetik, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany a.fleissner@tu-bs.de.
authors.forename: André
authors.initials: A
authors.lastname: Fleißner
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D059470:Tetraspanins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D059470:Tetraspanins
keywords: Neurospora crassa; autophagy; idi gene; tetraspanin; tsp3; vacuole