title: Application of Fluorescence Spectrometry With Multivariate Calibration to the Enantiomeric Recognition of Fluoxetine in Pharmaceutical Preparations.
abstract: Fluoxetine is the most prescribed antidepressant chiral drug worldwide. Its enantiomers have a different duration of serotonin inhibition. A novel simple and rapid method for determination of the enantiomeric composition of fluoxetine in pharmaceutical pills is presented. Specifically, emission, excitation, and synchronous fluorescence techniques were employed to obtain the spectral data, which with multivariate calibration methods, namely, principal component regression (PCR) and partial least square (PLS), were investigated. The chiral recognition of fluoxetine enantiomers in the presence of β-cyclodextrin was based on diastereomeric complexes. The results of the multivariate calibration modeling indicated good prediction abilities. The obtained results for tablets were compared with those from chiral HPLC and no significant differences are shown by Fisher's (F) test and Student's t-test. The smallest residuals between reference or nominal values and predicted values were achieved by multivariate calibration of synchronous fluorescence spectral data. This conclusion is supported by calculated values of the figure of merit.
authors.affiliation: Department of Analytical Chemistry, Institution Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Roman
authors.initials: R
authors.lastname: Poláček
authors.affiliation: Department of Analytical Chemistry, Institution Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Májek
authors.affiliation: Department of Analytical Chemistry, Institution Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Katarína
authors.initials: K
authors.lastname: Hroboňová
authors.affiliation: Department of Analytical Chemistry, Institution Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Jana
authors.initials: J
authors.lastname: Sádecká
mesh_terms: D000928:Antidepressive Agents / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005473:Fluoxetine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000928:Antidepressive Agents; D047392:beta-Cyclodextrins; D005473:Fluoxetine
keywords: chiral analysis; fluorescence spectrometry; fluoxetine enantiomers; multivariate calibration; pharmaceutical preparations; β-cyclodextrin