title: Effect of Bushen Yisui Capsule () on oligodendrocyte lineage genes 1 and 2 in mice with experimental autoimmune encephalomyelitis.
abstract: OBJECTIVE
  To study the effects of Bushen Yisui Capsule (, BSYSC) on the oligodendrocyte lineage genes (Olig) 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis (EAE) in order to explore the remyelination effect of BSYSC.
  
  
  METHODS
  The mice were randomly divided into normal control (NC), EAE model (EAE-M), prednisone acetate (PA, 6 mg/kg), BSYSC high-dose (3.02 g/kg) and BSYSC low-dose (1.51 g/kg) groups. The mice were induced by immunization with myelin oligodendrocyte glycoprotein (MOG) 35-55. The neurological function scores were assessed once daily. The pathological changes in mice brains were observed with hematoxylin-eosin (HE) staining and transmission electron microscope (TEM). The protein expressions of myelin basic protein (MBP), Olig1 and Olig2 in brains were measured by immunohistochemistry. The mRNA expressions of Olig1 and Olig 2 was also determined by quantitative real-time polymerase chain reaction.
  
  
  RESULTS
  Compared with the EAE-M mice, (1) the neurological function scores were significantly decreased in BSYSC-treated mice on days 22 to 40 (P<0.01); (2) the inflammatory cells and demyelination in brains were reduced in BSYSC-treated EAE mice; (3) the protein expression of MBP was markedly increased in BSYSC-treated groups on day 18 and 40 respectively (P<0.05 or P<0.01); (4) the protein expression of Olig1 was increased in BSYSC (3.02 g/kg)-treated EAE mice on day 40 (P<0.01). Protein and mRNA expression of Olig2 was increased in BSYSC-treated EAE mice on day 18 and 40 (P<0.01).
  
  
  CONCLUSIONS
  The effects of BSYSC on reducing demyelination and promoting remyelination might be associated with the increase of Olig1 and Olig2.
authors.affiliation: Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100059, China.|Department of Oncology, Guang'anmen Hospital of China Academy of Chinese Medical Sciences, Beijing, 100053, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100059, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100059, China.
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100059, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100059, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Qi
authors.affiliation: Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Kang-Ning
authors.initials: KN
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100059, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Fang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100059, China. tmwangl@ccmu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100050, China. yongpingf@hotmail.com.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Fan
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D001973:Bromodeoxyuridine / Q000378:metabolism; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D005260:Female; D005455:Fluorescent Antibody Technique; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine; D002214:Capsules; D004365:Drugs, Chinese Herbal; D063308:Myelin-Oligodendrocyte Glycoprotein; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D012333:RNA, Messenger; C494786:Olig1 protein, mouse; C512773:Olig2 protein, mouse; C527186:bushen yisui
keywords: Bushen Yisui Capsule; Chinese medicine; experimental autoimmune encephalomyelitis; myelin basic protein; oligodendrocyte lineage gene 1; oligodendrocyte lineage gene 2