title: Quercitrin-nanocoated titanium surfaces favour gingival cells against oral bacteria.
abstract: Many dental implants fail due to the infection and inflammation that walk hand in hand with poor healing and soft tissue integration. Titanium surfaces were nanocoated with quercitrin, a natural flavonoid, with the aim to improve soft tissue integration and increase dental implants success. Streptococcus mutans attachment and biofilm formation was analysed. Then, the anti-inflammatory properties and the potential of quercitrin-nanocoated surfaces to boost soft tissue regeneration were tested using human gingival fibroblasts. An inflammatory situation was mimicked using interleulin-1-beta. We found that quercitrin-nanocoated surfaces decreased initial bacterial adhesion while increasing human gingival fibroblasts attachment. Furthermore, quercitrin-nanocoated Ti increased collagen mRNA levels and decreased matrix metalloproteinase-1/tissue inhibitor of metalloproteinanse-1 mRNA ratio, which is related to a reduced metalloproteinase-mediated collagen degradation, while also decreasing the pro-inflammatory prostaglandin E2 release under basal and inflammatory conditions. These results suggest that quercitrin-nanocoated surfaces could enhance the soft tissue integration and increase dental implants success.
authors.affiliation: Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain.|Instituto de Investigación Sanitaria de Palma, 07010 Palma, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gomez-Florit
authors.affiliation: Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Badajoz, Spain.|Department of Applied Physics, Faculty of Science, University of Extremadura, Av. Elvas s/n, 06071 Badajoz, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Pacha-Olivenza
authors.affiliation: Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Badajoz, Spain.|Department of Biomedical Sciences, University of Extremadura, Badajoz, Spain.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Fernández-Calderón
authors.affiliation: Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain.|Instituto de Investigación Sanitaria de Palma, 07010 Palma, Spain.
authors.forename: Alba
authors.initials: A
authors.lastname: Córdoba
authors.affiliation: Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Badajoz, Spain.|Department of Applied Physics, Faculty of Science, University of Extremadura, Av. Elvas s/n, 06071 Badajoz, Spain.
authors.forename: Maria L
authors.initials: ML
authors.lastname: González-Martín
authors.affiliation: Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain.|Instituto de Investigación Sanitaria de Palma, 07010 Palma, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Monjo
authors.affiliation: Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of Balearic Islands, Palma de Mallorca, Spain.|Instituto de Investigación Sanitaria de Palma, 07010 Palma, Spain.
authors.forename: Joana M
authors.initials: JM
authors.lastname: Ramis
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D015921:Dental Implants / Q000382:microbiology*; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D005881:Gingiva / Q000166:cytology / Q000382:microbiology*; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D008875:Middle Aged; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D013295:Streptococcus mutans / Q000378:metabolism*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014025:Titanium; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D015921:Dental Implants; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; C012526:quercitrin; D011794:Quercetin; D014025:Titanium; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D015232:Dinoprostone