title: Antemortem stress regulates protein acetylation and glycolysis in postmortem muscle.
abstract: Although exhaustive research has established that preslaughter stress is a major factor contributing to pale, soft, exudative (PSE) meat, questions remain regarding the biochemistry of postmortem glycolysis. In this study, the influence of preslaughter stress on protein acetylation in relationship to glycolysis was studied. The data show that antemortem swimming significantly enhanced glycolysis and the total acetylated proteins in postmortem longissimus dorsi (LD) muscle of mice. Inhibition of protein acetylation by histone acetyltransferase (HAT) inhibitors eliminated stress induced increase in glycolysis. Inversely, antemortem injection of histone deacetylase (HDAC) inhibitors, trichostatin A (TSA) and nicotinamide (NAM), further increased protein acetylation early postmortem and the glycolysis. These data provide new insight into the biochemistry of postmortem glycolysis by showing that protein acetylation regulates glycolysis, which may participate in the regulation of preslaughter stress on glycolysis in postmortem muscle.
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, PR China; College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, PR China.
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, PR China; College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, PR China. Electronic address: yaoyao3153@aliyun.com.
authors.forename: Qingwu W
authors.initials: QW
authors.lastname: Shen
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, PR China. Electronic address: dequan_zhang0118@126.com.
authors.forename: Dequan
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000107:Acetylation; D000818:Animals; D006019:Glycolysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D011180:Postmortem Changes; D011770:Pyruvate Kinase / Q000378:metabolism; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D009124:Muscle Proteins; D011770:Pyruvate Kinase
keywords: HAT; HDAC; PSE; Postmortem glycolysis; Protein acetylation