title: Vitrified canine testicular cells allow the formation of spermatogonial stem cells and seminiferous tubules following their xenotransplantation into nude mice.
abstract: Belgian Malinois (BM), one of the excellent military dog breeds in South Korea, is usually castrated before sexual maturation. Therefore, the transfer of their genetic features to the next generation is difficult. To overcome this, testicular cells from 4-month-old BMs were frozen. Testicular cells were thawed after 3 months and cultured in StemPro-34 medium. Spermatogonial stem cell (SSC) characteristics were determined by the transplantation of the cultured germ cell-derived colonies (GDCs) into empty testes, containing only several endogenous SSCs and Sertoli cells, of immunodeficient mice, 4 weeks after busulfan treatment. Following the implantation, the transplanted cells localized in the basement membrane of the seminiferous tubules, and ultimately colonized the recipient testes. Xenotransplantation of GDCs together with testicular somatic cells conjugated with extracellular matrix (ECM), led to the formation of de novo seminiferous tubules. These seminiferous tubules were mostly composed of Sertoli cells. Some germ cells were localized in the basement membrane of seminiferous tubules. This study revealed that BM-derived SSCs, obtained from the castrated testes, might be a valuable tool for the transfer of BM genetic features to the next generation.
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Kyung Hoon
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Food Bioscience, RIBH, College of Biomedical &Health Science, Konkuk University, Chung-ju 380-701, Republic of Korea.
authors.forename: Won Young
authors.initials: WY
authors.lastname: Lee
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju-gun 565-851, Republic of Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju-gun 565-851, Republic of Korea.
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Jung Tae
authors.initials: JT
authors.lastname: Do
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam 463-836, Korea.
authors.forename: Jae Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Seongnam 463-836, Korea.
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Department of Animal Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
mesh_terms: D000818:Animals; D002066:Busulfan / Q000494:pharmacology; D002478:Cells, Cultured; D004285:Dogs; D005109:Extracellular Matrix / Q000378:metabolism; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D060888:Real-Time Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000166:cytology / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism; D013737:Testis / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D014157:Transcription Factors; D002066:Busulfan