title: In vitro osteogenic induction of human marrow-derived mesenchymal stem cells by PCL fibrous scaffolds containing dexamethazone-loaded chitosan microspheres.
abstract: This research reports the encapsulation of dexamethasone (Dex) within the chitosan microspheres (CSMs) embedded in a fibrous structure of poly(ɛ-caprolactone) (PCL) to provide a platform for osteogenic differentiation of human mesenchymal stem cells (hMSCs). Dex loaded CSMs were prepared by spray drying a mixture of chitosan and Dex. Then, they were electrospun with PCL solution to create a bilayer fibrous scaffold (PCL/CSMs-Dex). The CSMs act as good depots for sustained release of Dex over a period of 14 days, without noticeable burst release. This is mainly attributed to the core-shell structure of the final PCL/CSMs-Dex-matrix, which could prolong the release and eliminate the initial burst. The water contact angle of PCL scaffolds decreased from 141.4 ± 3.8 to 118.4 ± 7.6 in the presence of CSMs. Improved proliferation of hMSCs cultured on PCL/CSMs-Dex scaffolds was also evidenced. Furthermore, osteogenic assays showed an increase in alkaline phosphatase activity and mineral deposits. The expression of bone-specific genes also confirmed the osteogenic differentiation of cells cultured on these Dex-loaded core-shell structures. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1657-1667, 2016.
authors.affiliation: Biomedical Engineering Group, Chemical Engineering Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Noushin
authors.initials: N
authors.lastname: Omidvar
authors.affiliation: Biomedical Engineering Group, Chemical Engineering Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Ganji
authors.affiliation: Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Mohamadreza Baghaban
authors.initials: MB
authors.lastname: Eslaminejad
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D024042:Collagen Type I / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D065546:Drug Liberation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008863:Microspheres; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D011091:Polyesters; D015675:Osteocalcin; D053495:Osteopontin; C016240:polycaprolactone; D003907:Dexamethasone; D048271:Chitosan; D000469:Alkaline Phosphatase
keywords: chitosan; dexamethasone; electrospun; microspheres; polycaprolactone; scaffold