title: Recovery of laccase from processed Hericium erinaceus (Bull.:Fr) Pers. fruiting bodies in aqueous two-phase system.
abstract: The feasible use of aqueous two-phase systems (ATPSs) to establish a viable protocol for the recovery of laccase from processed Hericium erinaceus (Bull.:Fr.) Pers. fruiting bodies was evaluated. Cold-stored (4.00±1.00°C) H. erinaceus recorded the highest laccase activities of 2.02±0.04 U/mL among all the processed techniques. The evaluation was carried out in twenty-five ATPSs, which composed of polyethylene glycol (PEG) with various molecular weights and potassium phosphate salt solution to purify the protein from H. erinaceus. Optimum recovery condition was observed in the ATPS which contained 17% (w/w) PEG with a molecular weight of 8000 and 12.2% (w/w) potassium phosphate solution, at a volume ratio (VR) of 1.0. The use of ATPS resulted in one-single primary recovery stage process that produced an overall yield of 99% with a purification factor of 8.03±0.46. The molecular mass of laccases purified from the bottom phase was in the range of 55-66 kDa. The purity of the partitioned laccase was confirmed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Devamalini
authors.initials: D
authors.lastname: Rajagopalu
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia; Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
authors.forename: Pau Loke
authors.initials: PL
authors.lastname: Show
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: tanyeeshin@um.edu.my.
authors.forename: Yee Shin
authors.initials: YS
authors.lastname: Tan
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sekaran
authors.initials: S
authors.lastname: Muniandy
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; Mushroom Research Centre, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Vikineswary
authors.initials: V
authors.lastname: Sabaratnam
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D048690:Fruiting Bodies, Fungal / Q000201:enzymology*; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D010710:Phosphates; D017680:Potassium Compounds; D014867:Water; D011092:Polyethylene Glycols; C013216:potassium phosphate; D042845:Laccase
keywords: Aqueous two-phase; Hericium erinaceus; Laccase; Protein recovery; Purification; Separation