title: [Decreased expression levels of LAG-3 and CD49b on CD14+ cells in peripheral blood of patients with recurrent spontaneous abortion].
abstract: OBJECTIVE
  To investigate the expression levels of adhesion molecule CD49b and negative regulation molecule LAG-3 (CD223) on peripheral blood CD14(+) cells in patients with recurrent spontaneous abortion (RSA).
  
  
  METHODS
  Peripheral blood samples were collected from 7 normal female individuals and 12 patients with RSA, and then peripheral blood mononuclear cells (PBMCs) and plasma were separated from the peripheral blood via centrifugation. The expression levels of CD49b and LAG-3 on CD14(+) cells in PBMCs were detected by flow cytometry and the levels of interleukin 10 (IL-10) and transforming growth factor β (TGF-β) in plasma were detected by ELISA.
  
  
  RESULTS
  In the RSA patient group, there was no significant difference in the percentage of monocytes compared with that of the normal female group. The numbers of CD14(+) CD49b(+), CD14(+) LAG-3(+) and CD14(+) CD49b(+) LAG-3(+) cells in the RSA patient group were lower than those of the normal female group. The plasma level of TGF-β in the RSA patient group was lower than that of the normal female group. However, there was no significant difference in the plasma level of IL-10 between the two groups.
  
  
  CONCLUSIONS
  In RSA patients, the expression levels of CD49b and LAG-3 on CD14(+) monocytes and the plasma level of TGF-β decreased obviously compared with that of the normal females.
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hanyu
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Obstetrics and Gynecology, Tangdu Hospital, Xi'an 710038, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Tangdu Hospital, Xi'an 710038, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Obstetrics and Gynecology, Tangdu Hospital, Xi'an 710038, China. *Corresponding authors, E-mail: chenlh@fmmu.edu.cn.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China. *Corresponding authors, E-mail: xhwang_1968@126.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Chen
mesh_terms: D000026:Abortion, Habitual / Q000097:blood*; D000328:Adult; D015703:Antigens, CD / Q000097:blood*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D039421:Integrin alpha2 / Q000097:blood*; D016753:Interleukin-10 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000097:blood*; D016212:Transforming Growth Factor beta / Q000097:blood; D000093322:Lymphocyte Activation Gene 3 Protein
chemical_list: D015703:Antigens, CD; D039421:Integrin alpha2; D018950:Lipopolysaccharide Receptors; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D000093322:Lymphocyte Activation Gene 3 Protein; C000720326:Lag3 protein, human