title: Hairpin ribozyme mediated RNA recombination.
abstract: We have engineered a hairpin ribozyme that in a two-step reaction supports RNA recombination by catalysing the cleavage of two non-functional RNA precursors (first step) followed by the recombination of two of the cleavage fragments into a functional RNA (second step). The recombination product (yield: 76%) is a fully functional hammerhead ribozyme.
authors.affiliation: Ernst-Moritz-Arndt-Universität Greifswald, Institut für Biochemie, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. smueller@uni-greifswald.de.
authors.forename: Robert
authors.initials: R
authors.lastname: Hieronymus
authors.affiliation: Ernst-Moritz-Arndt-Universität Greifswald, Institut für Biochemie, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. smueller@uni-greifswald.de.
authors.forename: Simon Peter
authors.initials: SP
authors.lastname: Godehard
authors.affiliation: Ernst-Moritz-Arndt-Universität Greifswald, Institut für Biochemie, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. smueller@uni-greifswald.de.
authors.forename: Darko
authors.initials: D
authors.lastname: Balke
authors.affiliation: Ernst-Moritz-Arndt-Universität Greifswald, Institut für Biochemie, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. smueller@uni-greifswald.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Müller
mesh_terms: D002384:Catalysis; D012313:RNA / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry*; D011995:Recombination, Genetic
chemical_list: D016337:RNA, Catalytic; D012313:RNA