title: Unique antitumor property of the Mg-Ca-Sr alloys with addition of Zn.
abstract: In clinical practice, tumor recurrence and metastasis after orthopedic prosthesis implantation is an intensely troublesome matter. Therefore, to develop implant materials with antitumor property is extremely necessary and meaningful. Magnesium (Mg) alloys possess superb biocompatibility, mechanical property and biodegradability in orthopedic applications. However, whether they possess antitumor property had seldom been reported. In recent years, it showed that zinc (Zn) not only promote the osteogenic activity but also exhibit good antitumor property. In our present study, Zn was selected as an alloying element for the Mg-1Ca-0.5Sr alloy to develop a multifunctional material with antitumor property. We investigated the influence of the Mg-1Ca-0.5Sr-xZn (x = 0, 2, 4, 6 wt%) alloys extracts on the proliferation rate, cell apoptosis, migration and invasion of the U2OS cell line. Our results show that Zn containing Mg alloys extracts inhibit the cell proliferation by alteration the cell cycle and inducing cell apoptosis via the activation of the mitochondria pathway. The cell migration and invasion property were also suppressed by the activation of MAPK (mitogen-activated protein kinase) pathway. Our work suggests that the Mg-1Ca-0.5Sr-6Zn alloy is expected to be a promising orthopedic implant in osteosarcoma limb-salvage surgery for avoiding tumor recurrence and metastasis.
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Yuanhao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Southern Medical University, Guangzhou 510515, China.|Department of Orthopedics, Guangdong Key Lab of Orthopedic Technology and Implant, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, Guangdong 510010, China.
authors.forename: Guanping
authors.initials: G
authors.lastname: He
authors.affiliation: Department of Orthopedics, Guangdong Key Lab of Orthopedic Technology and Implant, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, Guangdong 510010, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Guangdong Key Lab of Orthopedic Technology and Implant, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, Guangdong 510010, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Guangdong Key Lab of Orthopedic Technology and Implant, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, Guangdong 510010, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Southern Medical University, Guangzhou 510515, China.|Department of Orthopedics, Guangdong Key Lab of Orthopedic Technology and Implant, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, Guangdong 510010, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.affiliation: Southern Medical University, Guangzhou 510515, China.|Department of Orthopedics, Guangdong Key Lab of Orthopedic Technology and Implant, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, Guangdong 510010, China.
authors.forename: Guan
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.|Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Southern Medical University, Guangzhou 510515, China.|Department of Orthopedics, Guangdong Key Lab of Orthopedic Technology and Implant, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, Guangdong 510010, China.
authors.forename: Qingshui
authors.initials: Q
authors.lastname: Yin
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000188:drug therapy / Q000473:pathology; D002118:Calcium / Q000737:chemistry; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D059565:G2 Phase Cell Cycle Checkpoints; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000188:drug therapy / Q000473:pathology; D013324:Strontium / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D000497:Alloys; D000970:Antineoplastic Agents; D020928:Mitogen-Activated Protein Kinases; D008274:Magnesium; D015032:Zinc; D002118:Calcium; D013324:Strontium