title: Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration.
abstract: Successful repair of a damaged corneal surface is a great challenge and may require the use of a scaffold that supports cell growth and differentiation. Amniotic membrane is currently used for this purpose, in spite of its limitations. A thin transparent silk fibroin film from non-mulberry Antheraea mylitta (Am) has been developed which offers to be a promising alternative. The silk scaffolds provide sufficient rigidity for easy handling, the scaffolds support the sprouting, migration, attachment and growth of epithelial cells and keratocytes from rat corneal explants; the cells form a cell sheet, preserve their phenotypes, express cytokeratin3 and vimentin respectively. The films also support growth of limbal stem cell evidenced by expression of ABCG2. The cell growth on the silk film and the amniotic membrane is comparable. The implanted film within the rabbit cornea remains transparent, stable. The clinical examination as well as histology shows absence of any inflammatory response or neovascularization. The corneal surface integrity is maintained; tear formation, intraocular pressure and electroretinography of implanted eyes show no adverse changes. The silk fibroin film from non-mulberry silk worms may be a worthy candidate for use as a corneal scaffold.
authors.affiliation: Department of Veterinary Surgery &Radiology, West Bengal University of Animal &Fishery Sciences, Kolkata-700037, West Bengal, India.
authors.forename: Sarbani
authors.initials: S
authors.lastname: Hazra
authors.affiliation: CSIR-Indian Institute of Chemical Biology, Kolkata-700032, West Bengal, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Nandi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, Kharagpur-721302, West Bengal, India.
authors.forename: Deboki
authors.initials: D
authors.lastname: Naskar
authors.affiliation: CSIR-Indian Institute of Chemical Biology, Kolkata-700032, West Bengal, India.
authors.forename: Rajdeep
authors.initials: R
authors.lastname: Guha
authors.affiliation: CSIR-Indian Institute of Chemical Biology, Kolkata-700032, West Bengal, India.
authors.forename: Sushovan
authors.initials: S
authors.lastname: Chowdhury
authors.affiliation: Department of Veterinary Surgery &Radiology, West Bengal University of Animal &Fishery Sciences, Kolkata-700037, West Bengal, India.
authors.forename: Nirparaj
authors.initials: N
authors.lastname: Pradhan
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Kharagpur, Kharagpur-721302, West Bengal, India.
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Kundu
authors.affiliation: CSIR-Indian Institute of Chemical Biology, Kolkata-700032, West Bengal, India.
authors.forename: Aditya
authors.initials: A
authors.lastname: Konar
mesh_terms: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000378:metabolism; D000650:Amnion / Q000637:transplantation; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003315:Cornea / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure; D060527:Corneal Keratocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004596:Electroretinography; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology*; D007429:Intraocular Pressure / Q000502:physiology; D053558:Keratin-3 / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009036:Moths / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012031:Refractometry; D012038:Regeneration / Q000187:drug effects*; D014746:Vimentin / Q000378:metabolism
chemical_list: D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D001672:Biocompatible Materials; D053558:Keratin-3; D014746:Vimentin; D005349:Fibroins