title: High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition.
abstract: Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.
authors.affiliation: German Rheumatism Research Center Berlin (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
authors.forename: Jakob
authors.initials: J
authors.lastname: Zimmermann
authors.affiliation: Department for Environmental Microbiology, Helmholtz Center for Environmental Research (UFZ), Leipzig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hübschmann
authors.affiliation: Department for Environmental Microbiology, Helmholtz Center for Environmental Research (UFZ), Leipzig, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Schattenberg
authors.affiliation: Department for Environmental Microbiology, Helmholtz Center for Environmental Research (UFZ), Leipzig, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Schumann
authors.affiliation: German Rheumatism Research Center Berlin (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
authors.forename: Pawel
authors.initials: P
authors.lastname: Durek
authors.affiliation: German Rheumatism Research Center Berlin (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
authors.forename: René
authors.initials: R
authors.lastname: Riedel
authors.affiliation: Charité - Universitätsmedizin Berlin, Medical Department (Gastroenterology, Infectious Diseases, Rheumatology), Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Marie
authors.initials: M
authors.lastname: Friedrich
authors.affiliation: Charité - Universitätsmedizin Berlin, Medical Department (Gastroenterology, Infectious Diseases, Rheumatology), Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Glauben
authors.affiliation: Charité - Universitätsmedizin Berlin, Medical Department (Gastroenterology, Infectious Diseases, Rheumatology), Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Siegmund
authors.affiliation: German Rheumatism Research Center Berlin (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Radbruch
authors.affiliation: Department for Environmental Microbiology, Helmholtz Center for Environmental Research (UFZ), Leipzig, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Müller
authors.affiliation: German Rheumatism Research Center Berlin (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Chang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification*; D003092:Colitis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005434:Flow Cytometry / Q000379:methods*; D000069196:Gastrointestinal Microbiome; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Flow cytometry; IBD; Microbiota; Single-cell analysis; T-cell transfer colitis