title: [Increased circulating Ly6Chigh monocyte subsets are correlated with cerebral infarct size in cerebral ischemia/reperfusion mouse models].
abstract: OBJECTIVE
  To investigate the dynamic changes of monocyte subsets after cerebral ischemia/reperfusion in mice and their correlations with infarct size and neurological defects.
  
  
  METHODS
  Thirty male C57BL/6 mice were randomly divided into two groups: middle cerebral artery occlusion/reperfusion (MCAO/R) group and Sham group. MCAO/R mouse models were induced via the intraluminal suture technique (so called filament or suture model). The modified Neurological Severity Scores (mNSS) was used to assess neurological function 6, 12 hours, 1, 2, 3 days after MCAO/R. Blood samples were collected 1, 2 and 3 days after surgery to determine the dynamic changes of Ly6C(high) and Ly6C(low) subsets by flow cytometry. Triphenyltetrazolium chloride (TTC) staining and HE staining of the brains were also performed on day 1, 2 and 3. The relationships between the changes of monocyte subsets and the cerebral infarct size and neurological scores were studied by correlation analysis.
  
  
  RESULTS
  Compared with the baseline, the proportion of Ly6C(high) monocytes significantly increased 1 day after MCAO/R surgery, reached the peak level on the following day and then declined. Compared with the Sham group, the proportion of Ly6C(high) monocytes went up obviously at each time point. TTC staining revealed that the infarct size increased markedly 2 days after surgery. The proportion of Ly6C(high) monocytes in the MCAO/R group was positively associated with the percentage of cerebral infarct size, and it also showed a positive correlation with neurological function deficit scores.
  
  
  CONCLUSIONS
  The dynamic changes of monocyte subsets after MCAO/R modeling revealed that Ly6C(high) subset peaked on day 2 after the operation and was correlated with cerebral infarct size.
authors.affiliation: School of Graduates, Tianjin Medical University, Tianjin 300070; Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China.
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Ji
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China. *Corresponding authors, E-mail: cardiolab@live.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Cardiovascular Disease Institute, Heart Center, Affiliated Hospital, Logistics University of the Chinese People's Armed Police Force, Tianjin 300162, China. *Corresponding authors, E-mail: xzhou@live.com.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D001921:Brain / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology; D002545:Brain Ischemia / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D002544:Cerebral Infarction / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D005434:Flow Cytometry; D020244:Infarction, Middle Cerebral Artery / Q000097:blood / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000378:metabolism*; D011897:Random Allocation; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D000950:Antigens, Ly; C494192:Ly-6C antigen, mouse