title: Construction of Nanodroplet/Adiposome and Artificial Lipid Droplets.
abstract: The lipid droplet (LD) is a cellular organelle that consists of a neutral lipid core with a monolayer-phospholipid membrane and associated proteins. Recent LD studies demonstrate its importance in metabolic diseases and biofuel development. However, the mechanisms governing its formation and dynamics remain elusive. Therefore, we developed an in vitro system to facilitate the elucidation of these mechanisms. We generated sphere-shaped structures with a neutral lipid core and a monolayer-phospholipid membrane by mechanically mixing neutral lipids and phospholipids followed by a two-step purification. We named the nanodroplet "adiposome". We then recruited LD structure-like/resident proteins to the adiposome, including the bacterial MLDS, Caenorhabditis elegans MDT-28/PLIN-1, or mammalian perilipin-2. In addition, adipose triglyceride lipase (ATGL) and apolipoprotein A1 (apo A-I) were recruited to adiposome. We termed the functional protein-coated adiposomes, Artificial Lipid Droplets (ALDs). With this experimental system, different proteins can be recruited to build ALDs for some biological goals and potential usage in drug delivery.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zhou
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Ma
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, China.|Department of Histology and Embryology, University of South China , Hengyang, Hunan 421001, China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Du
authors.affiliation: The Institute of Cardiovascular Sciences and Institute of Systems Biomedicine, School of Basic Medical Sciences, and Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education, Peking University Health Science Center , Beijing 100191, China.
authors.forename: Lemin
authors.initials: L
authors.lastname: Zheng
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
mesh_terms: D002001:Adipose Tissue, Brown / Q000737:chemistry*; D000818:Animals; D016632:Apolipoprotein A-I / Q000032:analysis; D017173:Caenorhabditis elegans / Q000737:chemistry; D029742:Caenorhabditis elegans Proteins / Q000032:analysis; D016503:Drug Delivery Systems; D066292:Lipid Droplets / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000070780:Perilipin-2 / Q000032:analysis; D010743:Phospholipids / Q000737:chemistry
chemical_list: D016632:Apolipoprotein A-I; D029742:Caenorhabditis elegans Proteins; D008055:Lipids; D008081:Liposomes; D000070780:Perilipin-2; D010743:Phospholipids
keywords: adiposome; artificial lipid droplet; lipid droplet; nanodroplet