title: Structure of a truncated form of leucine zipper II of JIP3 reveals an unexpected antiparallel coiled-coil arrangement.
abstract: JIP3 and JIP4, two highly related scaffolding proteins for MAP kinases, are binding partners for two molecular motors as well as for the small G protein ARF6. The leucine zipper II (LZII) region of JIP3/4 is the binding site for these three partners. Previously, the crystal structure of ARF6 bound to JIP4 revealed LZII in a parallel coiled-coil arrangement. Here, the crystal structure of an N-terminally truncated form of LZII of JIP3 alone shows an unexpected antiparallel arrangement. Using molecular dynamics and modelling, the stability of this antiparallel LZII arrangement, as well as its specificity for ARF6, were investigated. This study highlights that N-terminal truncation of LZII can change its coiled-coil orientation without affecting its overall stability. Further, a conserved buried asparagine residue was pinpointed as a possible structural determinant for this dramatic structural rearrangement. Thus, LZII of JIP3/4 is a versatile structural motif, modifications of which can impact partner recognition and thus biological function.
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Paola
authors.initials: P
authors.lastname: Llinas
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Chenon
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: T Quyen
authors.initials: TQ
authors.lastname: Nguyen
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal.
authors.forename: Catia
authors.initials: C
authors.lastname: Moreira
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Annélie
authors.initials: A
authors.lastname: de Régibus
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Coquard
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Ramos
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Guérois
authors.affiliation: UCIBIO, REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal.
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Fernandes
authors.affiliation: Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), CNRS, Université Paris-Sud, 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Ménétrey
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D016350:Leucine Zippers; D056004:Molecular Dynamics Simulation; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C485750:MAPK8IP3 protein, human; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments
keywords: JIP3; JIP4; antiparallel coiled coil; buried asparagine; crystal structure; leucine zipper II; molecular dynamics