title: Carrageenans effect on neutrophils alone and in combination with LPS in vitro.
abstract: Influence of sulfated red algal polysaccharides (κ-, λ-, and κ/β-carrageenans) and degraded derivative of κ/β-carrageenan on neutrophils/monocytes activation alone and in combination with lipopolysaccharide was investigated by means of determination of reactive oxygen species production, latex microparticles engulfment, total and extracellular myeloperoxidase induction and the analysis of silhouette and contour two-dimensional images of flattened cells. Carrageenans alone can activate neutrophils with much less potency than lipopolysaccharide (LPS) and the sulfation degree of carrageenans stipulates high activity in this role. On the other hand, carrageenans especially with low contents of sulfate groups are able to interfere with LPS in vitro resulting in reducing inter- and intracellular activation of neutrophils killing mechanisms. Further research is necessary to relate these findings to actions on the whole animal or human in vivo. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1603-1609, 2016.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, Vladivostok, 690022, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Sokolova
authors.affiliation: A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.|Department of Cell Biology and Genetics School of Natural Sciences, Far Eastern Federal University, Vladivostok, 690022, Russia.
authors.forename: Y
authors.initials: Y
authors.lastname: Karetin
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, Vladivostok, 690022, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Davydova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, Vladivostok, 690022, Russia.
authors.forename: A O
authors.initials: AO
authors.lastname: Byankina
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, Vladivostok, 690022, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Kalitnik
authors.affiliation: Medical Association of the Far East Branch of the Russian Academy of Sciences, St. Kirova, 95, Vladivostok, 690022, Russia.
authors.forename: L N
authors.initials: LN
authors.lastname: Bogdanovich
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch of the Russian Academy of Sciences, Prospect 100-let Vladivostoku, Vladivostok, 690022, Russia.
authors.forename: I M
authors.initials: IM
authors.lastname: Yermak
mesh_terms: D002351:Carrageenan / Q000737:chemistry / Q000494:pharmacology*; D004187:Disaccharides / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D006801:Humans; D007840:Latex; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology*; D008863:Microspheres; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*
chemical_list: D004187:Disaccharides; D007840:Latex; D008070:Lipopolysaccharides; D002351:Carrageenan
keywords: Carrageenan; analysis of silhouette images; flow cytometry; latex beads; myeloperoxidase; neutrophil; reactive oxygen species