title: Transcriptional regulation of cathelicidin genes in chicken bone marrow cells.
abstract: Cathelicidins form a family of vertebrate-specific immune molecules with an evolutionarily conserved gene structure. We analyzed the expression patterns of cathelicidin genes (CAMP, CATH3, and CATHB1) in chicken bone marrow cells (BMCs) and chicken embryonic fibroblasts (CEFs). We found that CAMP and CATHB1 were significantly up-regulated in BMCs, whereas the expression of CATH3 did not differ significantly between BMCs and CEFs. To study the mechanism underlying the up-regulation of cathelicidin genes in BMCs, we predicted the transcription factors (TFs) that bind to the 5'-flanking regions of cathelicidin genes. CEBPA, EBF1, HES1, MSX1, and ZIC3 were up-regulated in BMCs compared to CEFs. Subsequently, when a siRNA-mediated knockdown assay was performed for MSX1, the expression of CAMP and CATHB1 was decreased in BMCs. We also showed that the transcriptional activity of the CAMP promoter was decreased by mutation of the MSX1-binding sites present within the 5'-flanking region of CAMP. These results increase our understanding of the regulatory mechanisms controlling cathelicidin genes in BMCs.
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, 330-714, Republic of Korea.
authors.forename: Sang In
authors.initials: SI
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 330-714, Republic of Korea.
authors.forename: Hyun June
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea.
authors.forename: Mi-hyang
authors.initials: MH
authors.lastname: Jeon
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-4467.
authors.forename: Mi Ock
authors.initials: MO
authors.lastname: Lee
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea.
authors.forename: Jeom Sun
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea.
authors.forename: Ik-Soo
authors.initials: IS
authors.lastname: Jeon
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea pcs1778@korea.kr.
authors.forename: Sung June
authors.initials: SJ
authors.lastname: Byun
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism; D054804:Cathelicidins / Q000235:genetics* / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation
chemical_list: D023181:Antimicrobial Cationic Peptides; D030161:Avian Proteins; C504855:CMAP27 protein, chicken; D054804:Cathelicidins
keywords: CAMP; cathelicidin; chicken; promoter; transcription