title: Effect of Quercetin-loaded liposomes on induced oxidative stress in human spermatozoa.
abstract: A strategy to circumvent the poor polyphenols bioavailability is to load these compounds into liposomes. We evaluated the in vitro effects of quercetin (Q) and Q-loaded liposomes (QLL, 30, 50, 100μM) on motility, viability and chromatin integrity of swim-up selected human sperm. Antioxidant power was assayed against tert-butylhydroperoxide induced lipid peroxidation (LPO) using C11-BODIPY581/591 fluorescent probe and transmission electron microscopy. QLL showed decreased toxicity for sperm motility and viability and increased DNA damage compared to Q. The percentage of sperm with fluorescence, marker of LPO, was decreased in samples incubated with Q vs QLL (P<0.001). The ultrastructure of acrosomes and membranes was preserved with Q 30/100μM, whereas QLL did not prevent membrane injury. Q alone appeared more effective than Q incorporated into liposomes; however liposomes could be considered as carriers that may convey different compounds inside sperm; they may therefore represent a field of research rich of many applications.
authors.affiliation: Department of Molecular and Developmental Medicine, Policlinico Le Scotte, Viale Bracci 14, 53100 Siena, Italy. Electronic address: elena.moretti@unisi.it.
authors.forename: Elena
authors.initials: E
authors.lastname: Moretti
authors.affiliation: Department of Molecular and Developmental Medicine, Policlinico Le Scotte, Viale Bracci 14, 53100 Siena, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Mazzi
authors.affiliation: Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy; Centre for Colloid and Surface Science (CSGI), University of Florence, FI, Via della Lastruccia, 3, Sesto Fiorentino, 50019 , Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Bonechi
authors.affiliation: Centro di Microscopie Elettroniche "Laura Bonzi", ICCOM, Consiglio Nazionale delle Ricerche (CNR),Via Madonna del Piano,10 Firenze, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Salvatici
authors.affiliation: Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Iacoponi
authors.affiliation: Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy; Centre for Colloid and Surface Science (CSGI), University of Florence, FI, Via della Lastruccia, 3, Sesto Fiorentino, 50019 , Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Rossi
authors.affiliation: Department of Molecular and Developmental Medicine, Policlinico Le Scotte, Viale Bracci 14, 53100 Siena, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Collodel
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes; D008297:Male; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000008:administration & dosage* / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D020122:tert-Butylhydroperoxide
chemical_list: D000975:Antioxidants; D008081:Liposomes; D020122:tert-Butylhydroperoxide; D011794:Quercetin
keywords: Lipid peroxidation; Quercetin-loaded liposomes; Sperm chromatin; Sperm motility; Sperm viability; TEM