title: The impact of heterologous catalase expression and superoxide dismutase overexpression on enhancing the oxidative resistance in Lactobacillus casei.
abstract: Two heme-dependent catalase genes were amplified from genomic DNA of Lactobacillus plantarum WCFS1 (KatE1) and Lactobacillus brevis ATCC 367 (KatE2), respectively, and a manganese-containing superoxide dismutase from Lactobacillus casei MCJΔ1 (MnSOD) were cloned into plasmid pELX1, yielding pELX1-KatE1, pELX1-KatE2 and pELX1-MnSOD, then the recombinant plasmids were transferred into L. casei MCJΔ1. The strains of L. casei MCJΔ1/pELX1-KatE1 and L. casei MCJΔ1/pELX1-KatE2 were tolerant at 2 mM H2O2. The survival rates of L. casei MCJΔ1/pELX1-KatE1 and L. casei MCJΔ1/pELX1-KatE2 were 270-fold and 300-fold higher than that of the control strain on a short-term H2O2 exposure, and in aerated condition, the survival cells counts were 146- and 190-fold higher than that of the control strain after 96 h of incubation. Furthermore, L. casei MCJΔ1/pELX1-MnSOD was the best in three recombinants which was superior in the living cell viability during storage when co-storage with Lactobacillus delbrueckii subsp. lactis LBCH-1.
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy and Food Co., Ltd., 1518 Jiangchang Road (W), Shanghai, 200436, China.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China.
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Technology Center of Wuhan Xinxingdi Biotechnology Development Co., Ltd., 517 Luoshi South Road, Wuhan, 430070, China.
authors.forename: Yexia
authors.initials: Y
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Kunlin
authors.initials: K
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy and Food Co., Ltd., 1518 Jiangchang Road (W), Shanghai, 200436, China. chengjiema99@gmail.com.|Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China. chengjiema99@gmail.com.
authors.forename: Chengjie
authors.initials: C
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China. Zhengjun.chen@gmail.com.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Chen
mesh_terms: D002374:Catalase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D050296:Microbial Viability / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D014169:Transformation, Bacterial
chemical_list: D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Catalase; Heterologous expression; Lactobacillus casei; Oxidative resistance; Superoxide dismutase (SOD)