title: Generation of Transparent Zebrafish with Fluorescent Ovaries: A Living Visible Model for Reproductive Biology.
abstract: The transparent zebrafish enables researchers to study the morphology and distribution of cells and tissues in vivo. To capture the dynamic processes of germ cell proliferation and juvenile ovarian development in zebrafish in vivo, we established transgenic (TG) lines to allow us to monitor the changes in the ovaries of living fish. The original transgenic line with ovarian fluorescence was occasionally established. Although the cDNA integrated in the strain was constructed for the expression of enhanced green fluorescent protein (EGFP) driven by the medaka β-actin promoter, expression of EGFP is restricted to the oocytes and gills in adult fish. Mutant strains with transparent bodies, roy and ruby, were isolated in zebrafish. In this study, we crossed the TG strain with fluorescent ovary with transparent strains and established the TG (β-actin:EGFP);ruby strain. The strain is highly transparent, and the oocytes are easily observed in living fish. We identified a fluorescent tissue that might contain the undifferentiated germ cells close to the cloaca in the strain. This strain can be used for analysis of ovarian development in vivo.
authors.affiliation: 1 Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Afroza
authors.initials: A
authors.lastname: Akhter
authors.affiliation: 2 Department of Biology, Faculty of Science, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Ryo-Ichi
authors.initials: R
authors.lastname: Kumagai
authors.affiliation: 1 Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Shimi Rani
authors.initials: SR
authors.lastname: Roy
authors.affiliation: 2 Department of Biology, Faculty of Science, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Sanae
authors.initials: S
authors.lastname: Ii
authors.affiliation: 3 CREST Research Project, Japan Science and Technology Corporation , Kawaguchi, Japan .
authors.forename: Mika
authors.initials: M
authors.lastname: Tokumoto
authors.affiliation: 1 Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Babul
authors.initials: B
authors.lastname: Hossain
authors.affiliation: 1 Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1 Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Wanlada
authors.initials: W
authors.lastname: Klangnurak
authors.affiliation: 1 Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Takehiro
authors.initials: T
authors.lastname: Miyazaki
authors.affiliation: 1 Integrated Bioscience Section, Graduate School of Science and Technology, National University Corporation, Shizuoka University , Shizuoka, Japan .
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Tokumoto
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001947:Breeding; D004625:Embryo, Nonmammalian; D005260:Female; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D008297:Male; D010053:Ovary / Q000502:physiology*; D015027:Zebrafish
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins