title: Bacterial cellulose synthesis mechanism of facultative anaerobe Enterobacter sp. FY-07.
abstract: Enterobacter sp. FY-07 can produce bacterial cellulose (BC) under aerobic and anaerobic conditions. Three potential BC synthesis gene clusters (bcsI, bcsII and bcsIII) of Enterobacter sp. FY-07 have been predicted using genome sequencing and comparative genome analysis, in which bcsIII was confirmed as the main contributor to BC synthesis by gene knockout and functional reconstitution methods. Protein homology, gene arrangement and gene constitution analysis indicated that bcsIII had high identity to the bcsI operon of Enterobacter sp. 638; however, its arrangement and composition were same as those of BC synthesizing operon of G. xylinum ATCC53582 except for the flanking sequences. According to the BC biosynthesizing process, oxygen is not directly involved in the reactions of BC synthesis, however, energy is required to activate intermediate metabolites and synthesize the activator, c-di-GMP. Comparative transcriptome and metabolite quantitative analysis demonstrated that under anaerobic conditions genes involved in the TCA cycle were downregulated, however, genes in the nitrate reduction and gluconeogenesis pathways were upregulated, especially, genes in three pyruvate metabolism pathways. These results suggested that Enterobacter sp. FY-07 could produce energy efficiently under anaerobic conditions to meet the requirement of BC biosynthesis.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Ji
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biology Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, PR China.|Tianjin Key Laboratory of Microbial Functional Genomics, TEDA, Tianjin 300457, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Sibin
authors.initials: S
authors.lastname: Chen
authors.affiliation: Quality Control Department, Tsingtao Brewery Second Factory, Tsingtao Brewery CO., LTD, Qingdao 266000, PR China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Yueqing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D004579:Electron Transport; D004734:Energy Metabolism; D004754:Enterobacter / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D016680:Genome, Bacterial / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D005810:Multigene Family; D009876:Operon / Q000235:genetics; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002482:Cellulose; D005947:Glucose; D010100:Oxygen