title: TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis.
abstract: Propolis is a "natural" remedy with prominent biological activity, which is used as dietary supplement. In the absence of clinical studies that would substantiate these claims, information on the biological activity of propolis is valuable. This study comprises chromatographic, image processing and chemometric approach for phenolic profiling of Serbian, Croatian and Slovenian propolis test solutions. Modern thin-layer chromatography equipment in combination with software for image processing was applied for fingerprinting and data acquisition, whereas the principal component analysis was used as pattern recognition method. Characterization of phenolic profile was performed along with the determination of the botanical and geographical origin of propolis. High-performance thin-layer chromatograms reveal that Central and Southeastern European propolis samples are rich in flavonoids. In addition, phenolic compounds proved to be suitable markers for the determination of European propolis authenticity.
authors.affiliation: Chair of Analytical Chemistry and Centre of Excellence for Molecular Food Sciences, University of Belgrade - Faculty of Chemistry, PO Box 51, Belgrade 11158, Serbia dusankam@chem.bg.ac.rs.
authors.forename: Dušanka
authors.initials: D
authors.lastname: Milojković Opsenica
authors.affiliation: Innovation Centre of Faculty of Chemistry Ltd, Studentski trg 12-16, Belgrade 11000, Serbia.
authors.forename: Petar
authors.initials: P
authors.lastname: Ristivojević
authors.affiliation: Chair of Analytical Chemistry and Centre of Excellence for Molecular Food Sciences, University of Belgrade - Faculty of Chemistry, PO Box 51, Belgrade 11158, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Trifković
authors.affiliation: Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, Ljubljana SI-1000, Slovenia.
authors.forename: Irena
authors.initials: I
authors.lastname: Vovk
authors.affiliation: Department of Environmental Health, Faculty of Medicine, University of Rijeka, Braće Branchetta 20, Rijeka HR-51000, Croatia.
authors.forename: Dražen
authors.initials: D
authors.lastname: Lušić
authors.affiliation: Chair of Analytical Chemistry and Centre of Excellence for Molecular Food Sciences, University of Belgrade - Faculty of Chemistry, PO Box 51, Belgrade 11158, Serbia.
authors.forename: Živoslav
authors.initials: Ž
authors.lastname: Tešić
mesh_terms: D000818:Animals; D001516:Bees / Q000502:physiology; D002855:Chromatography, Thin Layer / Q000592:standards*; D017523:Croatia; D005419:Flavonoids / Q000302:isolation & purification*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000706:statistics & numerical data*; D010636:Phenols / Q000302:isolation & purification*; D032107:Populus; D025341:Principal Component Analysis; D011429:Propolis / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry; D055771:Serbia; D017524:Slovenia; D014885:Waxes / Q000737:chemistry
chemical_list: D005419:Flavonoids; D010636:Phenols; D012116:Resins, Plant; D014885:Waxes; C038228:beeswax; D011429:Propolis