title: An enantioselective NADP(+)-dependent alcohol dehydrogenase responsible for cooxidative production of (3S)-5-hydroxy-3-methyl-pentanoic acid.
abstract: A soil bacterium, Mycobacterium sp. B-009, is able to grow on racemic 1,2-propanediol (PD). The strain was revealed to oxidize 3-methyl-1,5-pentanediol (MPD) to 5-hydroxy-3-methyl-pentanoic acid (HMPA) during growth on PD. MPD was converted into an almost equimolar amount of the S-form of HMPA (S-HMPA) at 72%ee, suggesting the presence of an enantioselective MPD dehydrogenase (MPD-DH). As expected, an NADP(+)-dependent alcohol dehydrogenase, which catalyzes the initial step of MPD oxidation, was detected and purified from the cell-free extract. This enzyme was suggested to be a homodimeric medium-chain alcohol dehydrogenase/reductase (MDR). The catalytic and kinetic parameters indicated that MPD is the most suitable substrate for the enzyme. The enzyme was encoded by a 1047-bp gene (mpd1) and several mycobacterial strains were found to have putative MDR genes similar to mpd1. In a phylogenetic tree, MPD-DH formed an independent clade together with the putative MDR of Mycobacterium neoaurum, which produces opportunistic infections.
authors.affiliation: a Graduate School of Engineering , Yokohama National University , Yokohama , Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Takeda
authors.affiliation: a Graduate School of Engineering , Yokohama National University , Yokohama , Japan.
authors.forename: Aline Tiemi
authors.initials: AT
authors.lastname: Matsumura
authors.affiliation: a Graduate School of Engineering , Yokohama National University , Yokohama , Japan.
authors.forename: Kaishi
authors.initials: K
authors.lastname: Kurosaki
authors.affiliation: a Graduate School of Engineering , Yokohama National University , Yokohama , Japan.
authors.forename: Rajan Thapa
authors.initials: RT
authors.lastname: Chhetri
authors.affiliation: a Graduate School of Engineering , Yokohama National University , Yokohama , Japan.
authors.forename: Shigekazu
authors.initials: S
authors.lastname: Motomatsu
authors.affiliation: a Graduate School of Engineering , Yokohama National University , Yokohama , Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Suzuki
authors.affiliation: a Graduate School of Engineering , Yokohama National University , Yokohama , Japan.|b Department of Biochemistry , Usmanu Danfodiyo University , Sokoto , Nigeria.
authors.forename: Danladi Mahuta
authors.initials: DM
authors.lastname: Sahabi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D015870:Gene Expression; D006018:Glycols / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009161:Mycobacterium / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D016366:Open Reading Frames; D010084:Oxidation-Reduction; D010420:Pentanes / Q000737:chemistry / Q000378:metabolism; D010421:Pentanoic Acids / Q000737:chemistry / Q000378:metabolism*; D010802:Phylogeny; D019946:Propylene Glycol / Q000737:chemistry / Q000378:metabolism; D055503:Protein Multimerization; D016415:Sequence Alignment; D012988:Soil Microbiology; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D006018:Glycols; D010420:Pentanes; D010421:Pentanoic Acids; D019946:Propylene Glycol; D000429:Alcohol Oxidoreductases; C084155:alcohol dehydrogenase (NAD(P)+)
keywords: (3S)-5-Hydroxy-3-methyl-pentanoic acid; 3-Metyl-1,5-pentandiol; Alcohol dehydrogenase; Cooxidation; Mycobacterium