title: RNA Sequencing Reveals the Alteration of the Expression of Novel Genes in Ethanol-Treated Embryoid Bodies.
abstract: Fetal alcohol spectrum disorder is a collective term representing fetal abnormalities associated with maternal alcohol consumption. Prenatal alcohol exposure and related anomalies are well characterized, but the molecular mechanism behind this phenomenon is not well characterized. In this present study, our aim is to profile important genes that regulate cellular development during fetal development. Human embryonic carcinoma cells (NCCIT) are cultured to form embryoid bodies and then treated in the presence and absence of ethanol (50 mM). We employed RNA sequencing to profile differentially expressed genes in the ethanol-treated embryoid bodies from NCCIT vs. EB, NCCIT vs. EB+EtOH and EB vs. EB+EtOH data sets. A total of 632, 205 and 517 differentially expressed genes were identified from NCCIT vs. EB, NCCIT vs. EB+EtOH and EB vs. EB+EtOH, respectively. Functional annotation using bioinformatics tools reveal significant enrichment of differential cellular development and developmental disorders. Furthermore, a group of 42, 15 and 35 transcription factor-encoding genes are screened from all of the differentially expressed genes obtained from NCCIT vs. EB, NCCIT vs. EB+EtOH and EB vs. EB+EtOH, respectively. We validated relative gene expression levels of several transcription factors from these lists by quantitative real-time PCR. We hope that our study substantially contributes to the understanding of the molecular mechanism underlying the pathology of alcohol-mediated anomalies and ease further research.
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, Republic of Korea.
authors.forename: Chanchal
authors.initials: C
authors.lastname: Mandal
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, Republic of Korea.
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, Republic of Korea.
authors.forename: Jin Choul
authors.initials: JC
authors.lastname: Chai
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, Republic of Korea.
authors.forename: Seon Mi
authors.initials: SM
authors.lastname: Oh
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, Republic of Korea.
authors.forename: Young Seek
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Institute of Natural Science and Technology, Hanyang University, Ansan, Republic of Korea.
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.affiliation: Department of Molecular and Life Science, Hanyang University, Ansan, Republic of Korea.|Department of Bionanotechnology, Hanyang University, Seoul, Republic of Korea.
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002492:Central Nervous System Depressants / Q000494:pharmacology; D058732:Embryoid Bodies / Q000378:metabolism*; D054278:Embryonal Carcinoma Stem Cells / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006801:Humans; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol