title: Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8(T).
abstract: The present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8(T). This study considered six hydrocarbon mixtures that were tested for 22days at two different cultivation temperatures (4 and 15°C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC-FID (gas chromatography-flame ionization detection) hydrocarbon analysis. Results from DNA extraction and DAPI (4',6-diamidino-2-phenylindole) staining showed a linear increase during the first 18days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC-FID results revealed a substantial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15°C rather than 4°C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4°C.
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy. Electronic address: gabriella.gentile@iamc.cnr.it.
authors.forename: G
authors.initials: G
authors.lastname: Gentile
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Bonsignore
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy; Ph.D School in "Biology and Cellular Biotechnology" of University of Messina, Messina, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Santisi
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Catalfamo
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Napoli, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Giuliano
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Genovese
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy.
authors.forename: M M
authors.initials: MM
authors.lastname: Yakimov
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Denaro
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Genovese
authors.affiliation: Institute for Coastal Marine Environment (IAMC)-CNR of Messina, Messina, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Cappello
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006838:Hydrocarbons / Q000378:metabolism*; D009821:Oils; D010578:Petroleum / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D006838:Hydrocarbons; D009821:Oils; D010578:Petroleum; D014874:Water Pollutants, Chemical; C029662:JP5 jet fuel
keywords: Bioremediation; Hydrocarbonoclastic bacteria; Oil spill; Oleispira antarctica; Psychrophilic bacteria