title: Does the Location of Bruch's Membrane Opening Change Over Time? Longitudinal Analysis Using San Diego Automated Layer Segmentation Algorithm (SALSA).
abstract: OBJECTIVE
  We determined if the Bruch's membrane opening (BMO) location changes over time in healthy eyes and eyes with progressing glaucoma, and validated an automated segmentation algorithm for identifying the BMO in Cirrus high-definition coherence tomography (HD-OCT) images.
  
  
  METHODS
  We followed 95 eyes (35 progressing glaucoma and 60 healthy) for an average of 3.7 ± 1.1 years. A stable group of 50 eyes had repeated tests over a short period. In each B-scan of the stable group, the BMO points were delineated manually and automatically to assess the reproducibility of both segmentation methods. Moreover, the BMO location variation over time was assessed longitudinally on the aligned images in 3D space point by point in x, y, and z directions.
  
  
  RESULTS
  Mean visual field mean deviation at baseline of the progressing glaucoma group was -7.7 dB. Mixed-effects models revealed small nonsignificant changes in BMO location over time for all directions in healthy eyes (the smallest P value was 0.39) and in the progressing glaucoma eyes (the smallest P value was 0.30). In the stable group, the overall intervisit-intraclass correlation coefficient (ICC) and coefficient of variation (CV) were 98.4% and 2.1%, respectively, for the manual segmentation and 98.1% and 1.9%, respectively, for the automated algorithm.
  
  
  CONCLUSIONS
  Bruch's membrane opening location was stable in normal and progressing glaucoma eyes with follow-up between 3 and 4 years indicating that it can be used as reference point in monitoring glaucoma progression. The BMO location estimation with Cirrus HD-OCT using manual and automated segmentation showed excellent reproducibility.
authors.forename: Akram
authors.initials: A
authors.lastname: Belghith
authors.forename: Christopher
authors.initials: C
authors.lastname: Bowd
authors.forename: Felipe A
authors.initials: FA
authors.lastname: Medeiros
authors.forename: Naama
authors.initials: N
authors.lastname: Hammel
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Yang
authors.forename: Robert N
authors.initials: RN
authors.lastname: Weinreb
authors.forename: Linda M
authors.initials: LM
authors.lastname: Zangwill
mesh_terms: D000368:Aged; D000465:Algorithms; D016570:Bruch Membrane / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D005902:Glaucoma, Open-Angle / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D008297:Male; D013997:Time Factors; D041623:Tomography, Optical Coherence / Q000379:methods*; D014794:Visual Fields / Q000502:physiology*