title: Cloning, Phylogenetic Analysis, and Distribution of Free Fatty Acid Receptor GPR120 Expression along the Gastrointestinal Tract of Housing versus Grazing Kid Goats.
abstract: G-protein-coupled receptor 120 (GPR120) is reported as a long-chain fatty acid (LCFA) receptor that elicits free fatty acid (FFA) regulation on metabolism homeostasis. The study aimed to clone the gpr120 gene of goats (g-GPR120) and subsequently investigate phylogenetic analysis and tissue distribution throughout the digestive tracts of kid goats, as well as the effect of housing versus grazing (H vs G) feeding systems on GPR120 expression. Partial coding sequence (CDS) of g-GPR120 was cloned and submitted to NCBI (accession no. KU161270 ). Phylogenetic analysis revealed that g-GPR120 shared higher homology in both mRNA and amino acid sequences for ruminants than nonruminants. Immunochemistry, real-time PCR, and Western blot analysis showed that g-GPR120 was expressed throughout the digestive tracts of goats. The expression of g-GPR120 was affected by feeding system and age, with greater expression of g-GPR120 in the G group. It was concluded that the g-GPR120-mediated LCFA chemosensing mechanism is widely present in the tongue and gastrointestinal tract of goats and that its expression can be affected by feeding system and age.
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.|Graduate University of the Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ran
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.|Graduate University of the Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Hengzhi
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.|Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México , Toluca, Estado de México C.P. 50090, Mexico.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Chuanshe
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Shaoxun
authors.initials: S
authors.lastname: Tang
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Han
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.|Lethbridge Research Centre, Agriculture and Agri-Food Canada , Lethbridge, Alberta T1J 4B1, Canada.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: He
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Jinghe
authors.initials: J
authors.lastname: Kang
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Qiongxian
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Key Laboratory for Agro-Ecological Processes in Subtropical Region and South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences , Changsha, Hunan 410125, People's Republic of China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Lethbridge Research Centre, Agriculture and Agri-Food Canada , Lethbridge, Alberta T1J 4B1, Canada.
authors.forename: Karen A
authors.initials: KA
authors.lastname: Beauchemin
mesh_terms: D000595:Amino Acid Sequence; D000821:Animal Feed; D000818:Animals; D003001:Cloning, Molecular; D004032:Diet / Q000662:veterinary; D005230:Fatty Acids, Nonesterified; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D006041:Goats / Q000378:metabolism*; D006799:Housing, Animal; D010802:Phylogeny; D012333:RNA, Messenger / Q000737:chemistry; D043562:Receptors, G-Protein-Coupled / Q000032:analysis / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: C519853:FFAR4 protein, human; D005230:Fatty Acids, Nonesterified; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled
keywords: free fatty acid receptor; g-GPR120; gastrointestinal tract; goats; grazing and housing