title: Different response of photosystem II to short and long-term drought stress in Arabidopsis thaliana.
abstract: Short- and long-term drought stress on photosystem II (PSII) and oxidative stress were studied in Arabidopsis thaliana. Under drought stress, chlorophyll (Chl) content, Chl fluorescence, relative water content and oxygen evolution capacity gradually decreased, and the thylakoid structure was gradually damaged. Short-term drought stress caused a rapid disassembly of the light-harvesting complex II (LHCII). However, PSII dimers kept stable under the short-term drought stress and significantly decreased only after 15 days of drought stress. Immunoblotting analysis of the thylakoid membrane proteins showed that most of the photosystem proteins decreased after the stress, especially for Lhcb5, Lhcb6 and PsbQ proteins. However, surprisingly, PsbS significantly increased after the long-term drought stress, which is consistent with the substantially increased non-photochemical quenching (NPQ) after the stress. Our results suggest that the PSII-LHCII supercomplexes and LHCII assemblies play an important role in preventing photo-damages to PSII under drought stress.
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China. anty9826@163.com.
authors.forename: Yang-Er
authors.initials: YE
authors.lastname: Chen
authors.affiliation: Center of Analysis and Testing, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Su
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Jun-Mei
authors.initials: JM
authors.lastname: Cui
authors.affiliation: College of Resources Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China.
authors.forename: Huai-Yu
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: College of Resources Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. roundtree318@hotmail.com.
authors.forename: Shu
authors.identifier: http://orcid.org/0000-0001-6565-6914
authors.initials: S
authors.lastname: Yuan
mesh_terms: D017360:Arabidopsis / Q000502:physiology* / Q000528:radiation effects / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010766:Phosphorylation; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D060431:Thylakoid Membrane Proteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex; D017382:Reactive Oxygen Species; D060431:Thylakoid Membrane Proteins; D002734:Chlorophyll; D010100:Oxygen