title: New Rapid Method of DNA Isolation from Milk Somatic Cells.
abstract: Isolation of genomic DNA is one of the basic steps in many different molecular analyses. There are a few reports on methods of DNA isolation from milk, but many of them are time consuming and expensive, and require relatively large volumes of raw milk. In this study a rapid, sensitive, and efficient method of DNA extraction from milk somatic cells of various mammals (cattle, sheep, goats, horses) is presented. It was found that milk is a good source of genomic DNA, and to obtain a sufficient amount and quality of DNA, suitable for molecular analysis such as PCR, 10 mL of raw milk is sufficient. Thanks to this method, stress in animals can be reduced during collection of researched material. Therefore, this method could be widely used in molecular analyses.
authors.affiliation: a Department of Cattle Breeding , University of Agriculture in Krakow, Institute of Animal Science , Krakow , Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Pokorska
authors.affiliation: a Department of Cattle Breeding , University of Agriculture in Krakow, Institute of Animal Science , Krakow , Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Kułaj
authors.affiliation: a Department of Cattle Breeding , University of Agriculture in Krakow, Institute of Animal Science , Krakow , Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Dusza
authors.affiliation: a Department of Cattle Breeding , University of Agriculture in Krakow, Institute of Animal Science , Krakow , Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Żychlińska-Buczek
authors.affiliation: a Department of Cattle Breeding , University of Agriculture in Krakow, Institute of Animal Science , Krakow , Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Makulska
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006041:Goats; D006736:Horses; D008892:Milk / Q000737:chemistry* / Q000166:cytology*; D016133:Polymerase Chain Reaction; D012756:Sheep
chemical_list: D004247:DNA
keywords: DNA; isolation; mammals; milk somatic cells