title: Vaccination of Elk (Cervus canadensis) with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes Does Not Induce Adequate Protection against Experimental Brucella abortus Challenge.
abstract: In recent years, elk (Cervus canadensis) have been implicated as the source of Brucella abortus infection for numerous cattle herds in the Greater Yellowstone Area. In the face of environmental and ecological changes on the landscape, the range of infected elk is expanding. Consequently, the development of effective disease management strategies for wild elk herds is of utmost importance, not only for the prevention of reintroduction of brucellosis to cattle, but also for the overall health of the Greater Yellowstone Area elk populations. In two studies, we evaluated the efficacy of B. abortus strain RB51 over-expressing superoxide dismutase and glycosyltransferase for protecting elk from infection and disease caused by B. abortus after experimental infection with a virulent B. abortus strain. Our data indicate that the recombinant vaccine does not protect elk against brucellosis. Further, work is needed for development of an effective brucellosis vaccine for use in elk.
authors.affiliation: Wildlife Livestock Disease Investigations Team, Veterinary Services, Animal and Plant Health Inspection Services, United States Department of Agriculture Fort Collins, CO, USA.
authors.forename: Pauline
authors.initials: P
authors.lastname: Nol
authors.affiliation: Agricultural Research Services, United States Department of Agriculture, National Animal Disease Center Ames, IA, USA.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Olsen
authors.affiliation: Wildlife Livestock Disease Investigations Team, Veterinary Services, Animal and Plant Health Inspection Services, United States Department of Agriculture Fort Collins, CO, USA.
authors.forename: Jack C
authors.initials: JC
authors.lastname: Rhyan
authors.affiliation: Center for Molecular Medicine and Infectious Diseases, Virginia Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University Blacksburg, VA, USA.
authors.forename: Nammalwar
authors.initials: N
authors.lastname: Sriranganathan
authors.affiliation: Wildlife Livestock Disease Investigations Team, Veterinary Services, Animal and Plant Health Inspection Services, United States Department of Agriculture Fort Collins, CO, USA.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: McCollum
authors.affiliation: National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Services, United States Department of Agriculture Ames, IA, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Hennager
authors.affiliation: Department of Pathobiology and Microbiology, College of Veterinary Medicine and Biological Sciences, Colorado State University Fort Collins, CO, USA.
authors.forename: Alana A
authors.initials: AA
authors.lastname: Pavuk
authors.affiliation: Colorado Serum Company Denver, CO, USA.
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Sprino
authors.affiliation: Center for Molecular Medicine and Infectious Diseases, Virginia Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University Blacksburg, VA, USA.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Boyle
authors.affiliation: Colorado Serum Company Denver, CO, USA.
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Berrier
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biological Sciences, Colorado State University Fort Collins, CO, USA.
authors.forename: Mo D
authors.initials: MD
authors.lastname: Salman
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000276:immunology; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000276:immunology; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D003670:Deer / Q000276:immunology* / Q000382:microbiology; D005260:Female; D016695:Glycosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics; D014611:Vaccination / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D002004:Brucella Vaccine; D013482:Superoxide Dismutase; D016695:Glycosyltransferases
keywords: Brucella abortus; O-side chain; RB51; elk; glycosyltransferase; superoxide dismutase; wildlife vaccination