title: Identification and Characterization of Trichoderma Species Damaging Shiitake Mushroom Bed-Logs Infested by Camptomyia Pest.
abstract: The shiitake mushroom industry has suffered from Camptomyia (gall midges) pest, which feeds on the mycelium of shiitake mushroom during its cultivation. It has been postulated that fungal damage of shiitake bed-logs is associated with infestation by the insect pest, but this is not well understood. To understand the fungal damage associated with Camptomyia pest, various Trichoderma species were isolated, identified, and characterized. In addition to two previously known Trichoderma species, T. citrinoviride and T. deliquescens, two other Trichoderma species, T. harzianum and T. atroviride, were newly identified from the pestinfested bed-log samples obtained at three mushroom farms in Cheonan, Korea. Among these four species, T. harzianum was the most evident. The results of a chromogenic media-based assay for extracellular enzymes showed that these four species have the ability to produce amylase, carboxyl-methyl cellulase, avicelase, pectinase, and β-glucosidase, thus indicating that they can degrade wood components. A dual culture assay on PDA indicated that T. harzianum, T. atroviride, and T. citrinoviride were antagonistic against the mycelial growth of a shiitake strain (Lentinula edodes). Inoculation tests on shiitake bed-logs revealed that all four species were able to damage the wood of bed-logs. Our results provide evidence that the four green mold species are the causal agents involved in fungal damage of shiitake bed-logs infested by Camptomyia pest.
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Republic of Korea.
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Republic of Korea.
authors.forename: Hyuk Woo
authors.initials: HW
authors.lastname: Kwon
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Republic of Korea.
authors.forename: Yeo Hong
authors.initials: YH
authors.lastname: Yun
authors.affiliation: Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 31116, Republic of Korea.
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000681:Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D044602:Cellulases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics; D004175:Diptera / Q000382:microbiology*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D010802:Phylogeny; D011096:Polygalacturonase / Q000037:antagonists & inhibitors / Q000378:metabolism; D020942:Shiitake Mushrooms; D014242:Trichoderma / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D014934:Wood / Q000378:metabolism / Q000382:microbiology; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D000681:Amylases; D044602:Cellulases; D011096:Polygalacturonase; D001617:beta-Glucosidase
keywords: Camptomyia; Trichoderma damage; shiitake mushroom bed-logs