title: The linear and rotational motions of the fission yeast nucleus are governed by the stochastic dynamics of spatially distributed microtubules.
abstract: Dynamic nuclei are involved in a wide variety of fundamental biological processes including cell migration, cell division and fertilization. Here, we develop a mathematical model, in combination with live-cell imaging at high temporal resolution, to quantitatively elucidate how the linear and rotational motions of the nucleus are governed by the stochastic dynamics of the microtubule cytoskeleton. Our simulation and experimental results demonstrate that microtubule rescue and catastrophe frequencies are the decisive factors in regulating the nuclear movement. Lower rescue and catastrophe frequencies can lead to significantly larger angular and translational oscillations of the nucleus. In addition, our model also suggests that the stochastic dynamics of individual spatially distributed microtubules works collectively as a restoring force to maintain nuclear centering and hence ensures symmetric cell division, in excellent agreement with direct experimental observations.
authors.affiliation: Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Tsz Hin
authors.initials: TH
authors.lastname: Hui
authors.affiliation: CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, China; Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong, China. Electronic address: ylin@hku.hk.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, University of Science and Technology of China, Hefei, China; Department of Biochemistry, The University of Hong Kong, Pokfulam, Hong Kong, China. Electronic address: chuanhai@ustc.edu.cn.
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Fu
mesh_terms: D002455:Cell Division; D002465:Cell Movement; D002467:Cell Nucleus / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology; D008870:Microtubules / Q000502:physiology*; D008954:Models, Biological; D012568:Schizosaccharomyces / Q000502:physiology*
keywords: Microtubule; Modeling; Nucleus