title: Transit of H2O2 across the endoplasmic reticulum membrane is not sluggish.
abstract: Cellular metabolism provides various sources of hydrogen peroxide (H2O2) in different organelles and compartments. The suitability of H2O2 as an intracellular signaling molecule therefore also depends on its ability to pass cellular membranes. The propensity of the membranous boundary of the endoplasmic reticulum (ER) to let pass H2O2 has been discussed controversially. In this essay, we challenge the recent proposal that the ER membrane constitutes a simple barrier for H2O2 diffusion and support earlier data showing that (i) ample H2O2 permeability of the ER membrane is a prerequisite for signal transduction, (ii) aquaporin channels are crucially involved in the facilitation of H2O2 permeation, and (iii) a proper experimental framework not prone to artifacts is necessary to further unravel the role of H2O2 permeation in signal transduction and organelle biology.
authors.affiliation: Berufsfachschule Gesundheit Baselland, 4142 Münchenstein, Switzerland. Electronic address: christian.appenzeller@sbl.ch.
authors.forename: Christian
authors.initials: C
authors.lastname: Appenzeller-Herzog
authors.affiliation: Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest 1428, Hungary.
authors.forename: Gabor
authors.initials: G
authors.lastname: Bánhegyi
authors.affiliation: Department of Biophysics, School of Medicine, University of Saarland, 66421 Homburg, Germany.
authors.forename: Ivan
authors.initials: I
authors.lastname: Bogeski
authors.affiliation: Leonard Davis School of Gerontology of the Ethel Percy Andrus Gerontology Center; and Division of Molecular and Computational Biology, Department of Biological Sciences of the Dornsife College of Letters, Arts, and Sciences, The University of Southern California, Los Angeles, CA 90089-0191, USA; Division of Molecular and Computational Biology, Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, The University of Southern California, Los Angeles, CA 90089-0191, USA.
authors.forename: Kelvin J A
authors.initials: KJ
authors.lastname: Davies
authors.affiliation: Laboratoire Stress Oxydant et Cancers, CEA-Saclay, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif sur Yvette Cedex, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Delaunay-Moisan
authors.affiliation: Leonard Davis School of Gerontology of the Ethel Percy Andrus Gerontology Center; and Division of Molecular and Computational Biology, Department of Biological Sciences of the Dornsife College of Letters, Arts, and Sciences, The University of Southern California, Los Angeles, CA 90089-0191, USA.
authors.forename: Henry Jay
authors.initials: HJ
authors.lastname: Forman
authors.affiliation: Experimental and Molecular Pediatric Cardiology, German Heart Center Munich at the TU Munich, 80636 Munich, Germany.
authors.forename: Agnes
authors.initials: A
authors.lastname: Görlach
authors.affiliation: Faculty of Biochemistry and Molecular Medicine, University of Oulu, 90210 Oulu, Finland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kietzmann
authors.affiliation: Vascular Biology Laboratory, Heart Institute, University of São Paulo School of Medicine, CEP 05403-000 São Paulo, Brazil.
authors.forename: Francisco
authors.initials: F
authors.lastname: Laurindo
authors.affiliation: Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest 1428, Hungary.
authors.forename: Eva
authors.initials: E
authors.lastname: Margittai
authors.affiliation: INRES-Chemical Signalling, University of Bonn, 53113 Bonn, Germany.
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Meyer
authors.affiliation: Institute for Biochemistry, University of Cologne, 50674 Cologne, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Riemer
authors.affiliation: Institute for Health Science and Technology, Aalborg University, DK-9220 Aalborg, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Rützler
authors.affiliation: Department of Cell Biology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada T6G2H7.
authors.forename: Thomas
authors.initials: T
authors.lastname: Simmen
authors.affiliation: Protein Transport and Secretion Unit, Division of Genetics and Cell Biology, IRCCS, Ospedale San Raffaele/Universita' Vita-Salute San Raffaele, 20132 Milan, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Sitia
authors.affiliation: Laboratoire Stress Oxydant et Cancers, CEA-Saclay, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif sur Yvette Cedex, France.
authors.forename: Michel B
authors.initials: MB
authors.lastname: Toledano
authors.affiliation: Erasmus University Medical Center, Department of Hematology, PO Box 2040, Rotterdam, The Netherlands.
authors.forename: Ivo P
authors.initials: IP
authors.lastname: Touw
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004058:Diffusion; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide