title: Fabrication, characterization and biomedical application of two-nozzle electrospun polycaprolactone/zein-calcium lactate composite nonwoven mat.
abstract: The objective of the current work is to incorporate calcium lactate (CL) into polycaprolactone (PCL)/zein composite micro/nanofibrous scaffolds via electrospinning to engineer bone tissue. In this study, a composite micro/nano fibrous scaffold was fabricated using a single two-nozzle electrospinning system to combine indicative nanofibers from a blended solution of zein-CL and micro-sized fibers from a PCL solution. Incorporation of the CL into the PCL/zein fibers were shown to improve the wettability, tensile strength and biological activity of the composite mats. Moreover, the composite mats have a high efficiency to nucleate calcium phosphate from simulated body fluid (SBF) solution. An in vitro cell culture with osteoblast cells demonstrated that the electrospun composite mats possessed improved biological properties, including a better cell adhesion, spread and proliferation. This study has demonstrated that the PCL/zein-CL composite provides a simple platform to fabricate a new biomimetic scaffold for bone tissue engineering, which can recapitulate both the morphology of extracellular matrix and composition of the bone.
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Center for Translational Medicine Research and Development, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, China.
authors.forename: Nina
authors.initials: N
authors.lastname: Liao
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.
authors.forename: Mahesh Kumar
authors.initials: MK
authors.lastname: Joshi
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Arjun Prasad
authors.initials: AP
authors.lastname: Tiwari
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: biochan@jbnu.ac.kr.
authors.forename: Chan-Hee
authors.initials: CH
authors.lastname: Park
authors.affiliation: Department of Bionanosystem Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: chskim@jbnu.ac.kr.
authors.forename: Cheol Sang
authors.initials: CS
authors.lastname: Kim
mesh_terms: D017610:Calcium Compounds / Q000737:chemistry*; D002478:Cells, Cultured; D006801:Humans; D007773:Lactates / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D015028:Zein / Q000737:chemistry*
chemical_list: D017610:Calcium Compounds; D007773:Lactates; D011091:Polyesters; C016240:polycaprolactone; C110051:calcium lactate; D015028:Zein
keywords: Biomedical application; Bone tissue; Calcium lactate; Micro-nano fibers; Two-nozzle electrospinning