title: Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles.
abstract: The therapeutic potential of antibodies has not been fully exploited as they fail to cross cell membrane. In this article, we have tested the possibility of using plant virus based nanoparticles for intracellular delivery of antibodies. For this purpose, Sesbania mosaic virus coat protein (CP) was genetically engineered with the B domain of Staphylococcus aureus protein A (SpA) at the βH-βI loop, to generate SeMV loop B (SLB), which self-assembled to virus like particles (VLPs) with 43 times higher affinity towards antibodies. CP and SLB could internalize into various types of mammalian cells and SLB could efficiently deliver three different monoclonal antibodies-D6F10 (targeting abrin), anti-α-tubulin (targeting intracellular tubulin) and Herclon (against HER2 receptor) inside the cells. Such a mode of delivery was much more effective than antibodies alone treatment. These results highlight the potential of SLB as a universal nanocarrier for intracellular delivery of antibodies.
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Karnataka, India.
authors.forename: Ambily
authors.initials: A
authors.lastname: Abraham
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Karnataka, India.
authors.forename: Usha
authors.initials: U
authors.lastname: Natraj
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Karnataka, India.
authors.forename: Anjali A
authors.initials: AA
authors.lastname: Karande
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Karnataka, India.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Gulati
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Karnataka, India.
authors.forename: Mathur R N
authors.initials: MR
authors.lastname: Murthy
authors.affiliation: Theramyt Novobiologics Pvt. Ltd., Karnataka, India.
authors.forename: Sathyabalan
authors.initials: S
authors.lastname: Murugesan
authors.affiliation: Theramyt Novobiologics Pvt. Ltd., Karnataka, India.
authors.forename: Pavithra
authors.initials: P
authors.lastname: Mukunda
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Karnataka, India.
authors.forename: Handanahal S
authors.initials: HS
authors.lastname: Savithri
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D006367:HeLa Cells; D006801:Humans; D008546:Melanoma, Experimental; D051379:Mice; D009029:Mosaic Viruses; D055503:Protein Multimerization; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D049931:Sesbania / Q000821:virology; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000378:metabolism; D014771:Virion
chemical_list: D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A