title: Lipid accumulation by oleaginous and non-oleaginous yeast strains in nitrogen and phosphate limitation.
abstract: We investigated the possibility of utilizing both oleaginous yeast species accumulating large amounts of lipids (Yarrowia lipolytica, Rhodotorula glutinis, Trichosporon cutaneum, Candida sp.) and traditional biotechnological non-oleaginous ones characterized by high biomass yield (Kluyveromyces polysporus, Torulaspora delbrueckii, Saccharomyces cerevisiae) as potential producers of biofuel-utilizable and nutritionally valuable lipids. The main objective was to increase lipid accumulation by increasing C/P ratio together with higher C/N ratio, while maintaining high biomass yield. The C/N ratio of 30 was found to lead to higher biomass content and the total lipid content increased significantly with higher C/P ratio. With higher ratios of both C/N and C/P, the content of monounsaturated fatty acids (FAs) in cell lipids increased while polyunsaturated FAs decreased. Oleaginous yeast species had a lower proportion of unsaturated FAs (approx. 80 %) than non-oleaginous strains (approx. 90 %). At a C/N ratio of 30 and C/P ratio 1043, T. cutaneum produced a high amount of ω-6 unsaturated linoleic acid, the precursor of some prostaglandins, leukotrienes, and thromboxanes, while Candida sp. and K. polysporus accumulated a high content of palmitoleic acid.
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology, Technická 5, CZ-166 28, Prague 6, Czech Republic.
authors.forename: Irena
authors.initials: I
authors.lastname: Kolouchová
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology, Technická 5, CZ-166 28, Prague 6, Czech Republic. olga.matatkova@vscht.cz.
authors.forename: Olga
authors.identifier: http://orcid.org/0000-0002-3646-9738
authors.initials: O
authors.lastname: Maťátková
authors.affiliation: Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Sigler
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology, Technická 5, CZ-166 28, Prague 6, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Masák
authors.affiliation: Department of Biotechnology, University of Chemistry and Technology, Technická 5, CZ-166 28, Prague 6, Czech Republic.|Institute of Microbiology, CAS, Vídeňská 1083, 142 20, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Řezanka
mesh_terms: D003470:Culture Media / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D009584:Nitrogen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D015003:Yeasts / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D010710:Phosphates; D009584:Nitrogen