title: Chemical Tools to Investigate Mechanisms Associated with HSP90 and HSP70 in Disease.
abstract: The chaperome is a large and diverse protein machinery composed of chaperone proteins and a variety of helpers, such as the co-chaperones, folding enzymes, and scaffolding and adapter proteins. Heat shock protein 90s and 70s (HSP90s and HSP70s), the most abundant chaperome members in human cells, are also the most complex. As we have learned to appreciate, their functions are context dependent and manifested through a variety of conformations that each recruit a subset of co-chaperone, scaffolding, and folding proteins and which are further diversified by the posttranslational modifications each carry, making their study through classic genetic and biochemical techniques quite a challenge. Chemical biology tools and techniques have been developed over the years to help decipher the complexities of the HSPs and this review provides an overview of such efforts with focus on HSP90 and HSP70.
authors.affiliation: Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA.
authors.forename: Liza
authors.initials: L
authors.lastname: Shrestha
authors.affiliation: Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA.
authors.forename: Hardik J
authors.initials: HJ
authors.lastname: Patel
authors.affiliation: Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA; Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA. Electronic address: chiosisg@mskcc.org.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Chiosis
mesh_terms: D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000032:analysis / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D015335:Molecular Probes