title: Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM.
abstract: Bacterial surface layers (S-layers) are crystalline arrays of self-assembling proteinaceous subunits called S-layer proteins (Slps) that comprise the outermost layer of the cell envelope. Many additional proteins that are associated with or embedded within the S-layer have been identified in Lactobacillus acidophilus NCFM, an S-layer-forming bacterium that is widely used in fermented dairy products and probiotic supplements. One putative S-layer-associated protein (SLAP), LBA0191, was predicted to mediate adhesion to fibronectin based on the in silico detection of a fibronectin-binding domain. Fibronectin is a major component of the extracellular matrix (ECM) of intestinal epithelial cells. Adhesion to intestinal epithelial cells is considered an important trait for probiotic microorganisms during transit and potential association with the intestinal mucosa. To investigate the functional role of LBA0191 (designated FbpB) in L. acidophilus NCFM, an fbpB-deficient strain was constructed. The L. acidophilus mutant with a deletion off bpB lost the ability to adhere to mucin and fibronectin in vitro Homologues off bpB were identified in five additional putative S-layer-forming species, but no homologues were detected in species outside theL. acidophilus homology group.
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Hymes
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA.|Graduate Program in Microbiology, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Brant R
authors.initials: BR
authors.lastname: Johnson
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA.|Graduate Program in Microbiology, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Barrangou
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA klaenhammer@ncsu.edu.|Graduate Program in Microbiology, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Todd R
authors.initials: TR
authors.lastname: Klaenhammer
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D007779:Lactobacillus acidophilus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009154:Mutation; D010802:Phylogeny; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D005353:Fibronectins; D008562:Membrane Glycoproteins; C055764:S-layer proteins