title: Suppressor Properties of Human CD8(+)CD28(-) T Cells in Mixed Leukocyte Reaction are not Affected by CsA and RAPA.
abstract: Human CD8(+)CD28(-) T suppressor cells were previously shown to be involved in the control of the immune response to transplanted allografts. It seems essential to examine how immunosuppressive drugs influence these cells. However, the CD8(+)CD28(-) population contains both suppressor (Ts) and cytotoxic (Tc) T cells, and the phenotype of the Ts subpopulation has not been identified explicitly. It is proposed that the transcription factor FOXP3 may be helpful in distinguishing the Ts and Tc subpopulations. The aim of this study was to evaluate the influence of the immunosuppressive drugs cyclosporine A (CsA) and rapamycin (RAPA) on the level, suppressor properties, and phenotype of human CD8(+)CD28(-) T cells in vitro. The model used was the mixed leukocyte reaction performed with peripheral blood mononuclear cells from healthy volunteers. It was observed that CD8(+)CD28(-) T cells from cultures with CsA or RAPA had similar suppressor properties to cells from control cultures, although the drugs influenced the expression of FOXP3. CsA and RAPA did not interfere with the suppressor properties of human CD8(+)CD28(-) T cells in vitro, although they affected the expression of the FOXP3 molecule.
authors.affiliation: Department of Immunology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Korecka-Polak
authors.affiliation: Department of Immunology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bocian
authors.affiliation: Department of Immunology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Pachówka
authors.affiliation: Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Jałbrzykowska
authors.affiliation: Department of Immunology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland. gkorczak-k@biol.uw.edu.pl.|Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland. gkorczak-k@biol.uw.edu.pl.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Korczak-Kowalska
mesh_terms: D000293:Adolescent; D000328:Adult; D018106:CD28 Antigens / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D016572:Cyclosporine / Q000008:administration & dosage* / Q000737:chemistry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008875:Middle Aged; D010641:Phenotype; D020123:Sirolimus / Q000008:administration & dosage* / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult
chemical_list: D018106:CD28 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D007166:Immunosuppressive Agents; D016572:Cyclosporine; D020123:Sirolimus
keywords: CD8+CD28−; Cyclosporine A; FOXP3; Rapamycin; Suppressor T cells