title: Effect of turmeric on colon histology, body weight, ulcer, IL-23, MPO and glutathione in acetic-acid-induced inflammatory bowel disease in rats.
abstract: BACKGROUND
  This study investigates the protective effects of turmeric (Curcuma longa, CL) on acetic acid-induced colitis in rats.
  
  
  METHODS
  Inflammatory bowel disease (IBD) was induced in male Wistar rats by intra-rectal administration of 1 ml of 4% acetic acid at 8 cm proximal to the anus for 30 s. Curcuma longa (CL) powder, (1, 10, or 100 mg/kg/day) was administered for either 3 days before or after IBD for 7 days. The body weight, macroscopic and microscopic analysis of the colon of CL-treated IBD rats and that of control rats (no IBD, no CL) were performed on 0 day, 2, 4 and 7th day. Myeloperoxidase (MPO), IL-23 and glutathione levels in control, untreated and treated rats were measured by ELISA.
  
  
  RESULTS
  CL significantly (P < 0.05) improved IBD-induced reduction in mean body weight and mean macroscopic ulcer score. Administration of CL also significantly (P < 0.01) reduced the mean microscopic ulcer score when compared to untreated IBD control. Intake of CL by rats resulted in a significant (P < 0.05) increase in the mean serum glutathione level compared to untreated control. CL reduced both MPO and IL-23 levels in the colonic mucosa of the rat.
  
  
  CONCLUSIONS
  CL improved body weight gain, mean macroscopic and microscopic ulcer scores in the colon of rats suffering from acetic acid-induced IBD. CL reduced both MPO and IL-23 in the mucosa of the colon. The increase in the mean serum glutathione level may help in the reduction of oxidative stress associated with IBD.
authors.affiliation: Department of Pharmacology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE. sbastaki@uaeu.ac.ae.
authors.forename: Salim M A
authors.initials: SMA
authors.lastname: Bastaki
authors.affiliation: Department of Pharmacology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
authors.forename: Mohammed Majed
authors.initials: MM
authors.lastname: Al Ahmed
authors.affiliation: Department of Pharmacology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Al Zaabi
authors.affiliation: Department of Pharmacology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
authors.forename: Naheed
authors.initials: N
authors.lastname: Amir
authors.affiliation: Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE. eadeghate@uaeu.ac.ae.
authors.forename: Ernest
authors.initials: E
authors.lastname: Adeghate
mesh_terms: D019342:Acetic Acid; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D001835:Body Weight / Q000187:drug effects*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D030024:Curcuma; D005978:Glutathione / Q000378:metabolism*; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D053759:Interleukin-23 / Q000097:blood*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000097:blood*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D017208:Rats, Wistar; D014456:Ulcer
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D053759:Interleukin-23; D010936:Plant Extracts; D009195:Peroxidase; D005978:Glutathione; D019342:Acetic Acid