title: Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels.
abstract: Fusarium verticillioides is considered one of the most important global sources of fumonisins contamination in food and feed. Corn is one of the main commodities produced in the Northeastern Region of Brazil. The present study investigated potential mycotoxigenic fungal strains belonging to the F. verticillioides species isolated from corn kernels in 3 different Regions of the Brazilian State of Pernambuco. A polyphasic approach including classical taxonomy, molecular biology, MALDI-TOF MS and MALDI-TOF MS/MS for the identification and characterisation of the F. verticillioides strains was used. Sixty F. verticillioides strains were isolated and successfully identified by classical morphology, proteomic profiles of MALDI-TOF MS, and by molecular biology using the species-specific primers VERT-1 and VERT-2. FUM1 gene was further detected for all the 60 F. verticillioides by using the primers VERTF-1 and VERTF-2 and through the amplification profiles of the ISSR regions using the primers (GTG)₅ and (GACA)₄. Results obtained from molecular analysis shown a low genetic variability among these isolates from the different geographical regions. All of the 60 F. verticillioides isolates assessed by MALDI-TOF MS/MS presented ion peaks with the molecular mass of the fumonisin B1 (721.83 g/mol) and B2 (705.83 g/mol).
authors.affiliation: Postgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, Brazil. susicchang@hotmail.com.
authors.forename: Susane
authors.initials: S
authors.lastname: Chang
authors.affiliation: Postgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, Brazil. mariele_carneiro@hotmail.com.
authors.forename: Mariele Porto
authors.initials: MP
authors.lastname: Carneiro-Leão
authors.affiliation: Postgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, Brazil. benny.oliveira74@hotmail.com.
authors.forename: Benny Ferreira
authors.initials: BF
authors.lastname: de Oliveira
authors.affiliation: Postgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, Brazil. cristina.motta@ufpe.br.
authors.forename: Cristina
authors.initials: C
authors.lastname: Souza-Motta
authors.affiliation: CEB-Centre of Biological Engineering, Micoteca da Universidade do Minho, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal. nelson@ie.uminho.pt.
authors.forename: Nelson
authors.initials: N
authors.lastname: Lima
authors.affiliation: Department of Chemical Sciences and Natural Resources, CIBAMA, BIOREN-UFRO, Faculty of Engineering and Sciences, Universidad de La Frontera, Av. Francisco Salazar, 01145 Temuco, Chile. cledir.santos@ufrontera.cl.
authors.forename: Cledir
authors.initials: C
authors.lastname: Santos
authors.affiliation: Postgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, Brazil. netinti@hotmail.com.
authors.forename: Neiva Tinti
authors.initials: NT
authors.lastname: de Oliveira
mesh_terms: D001938:Brazil; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000032:analysis; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification*; D005800:Genes, Fungal; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D037341:Fumonisins; C056934:fumonisin B2; C056933:fumonisin B1
keywords: fumonisins; genetic study; mycotoxigenic fungi; mycotoxin