title: [High-dose neuropeptide Y inhibits proliferation and promotes differentiation of human adipose-derived stem cells].
abstract: OBJECTIVE
  To investigate the impact of neuropeptide Y (NPY) at different concentrations on the proliferation and differentiation of human adipose-derived stem cells (hADSCs).
  
  
  METHODS
  The hADSCs were separated and incubated with NPY at a range of concentrations 10(-6)-10(-15) mol/L. MTT assay was used to detect cell proliferation. Following the treatment of complete medium, NPY and insulin separately, cell differentiation was observed by Oil red O staining. In addition, Western blotting was performed to exam the levels of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), cell death-inducing dFF45-like effector c (Cidec) and receptor interacting protein 140 (RIP140).
  
  
  RESULTS
  MTT assay revealed that 10(-11)-10(-15) mol/L NPY stimulated the proliferation of hADSCs, while high-dose NPY (10(-6)-10(-10) mol/L) inhibited the cell proliferation. NPY (10(-7), 10(-9), 10(-11) mol/L) induced the differentiation of hADSCs. The expression levels of related adipocyte markers (PPARγ, C/EBPα), mature white adipose tissue specific markers (Cidec, RIP140) increased at the presence of NPY (10(-7), 10(-9), 10(-11) mol/L).
  
  
  CONCLUSIONS
  Low-dose NPY could stimulate the proliferation of hADSCs, and high-dose NPY could inhibit the proliferation and promote the differentiation of hADSCs.
authors.affiliation: Department of Head & Neck for Old Cadre, Tumor Hospital of Xiangya Medical College, Changsha 410013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Pingan
authors.initials: P
authors.lastname: Hu
authors.affiliation: Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liang
authors.affiliation: Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Nutrition, Third Xiangya Hospital, Central South University, Changsha 410013, China. *Corresponding author, E-mail: liumin330@hotmail.com.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009478:Neuropeptide Y / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000378:metabolism; D000078723:Nuclear Receptor Interacting Protein 1; D047495:PPAR gamma / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D022763:CCAAT-Enhancer-Binding Protein-alpha; C472088:CIDEC protein, human; C000627127:NRIP1 protein, human; D009478:Neuropeptide Y; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D047495:PPAR gamma; D011506:Proteins