title: Global Identification of the Full-Length Transcripts and Alternative Splicing Related to Phenolic Acid Biosynthetic Genes in Salvia miltiorrhiza.
abstract: Salvianolic acids are among the main bioactive components in Salvia miltiorrhiza, and their biosynthesis has attracted widespread interest. However, previous studies on the biosynthesis of phenolic acids using next-generation sequencing platforms are limited with regard to the assembly of full-length transcripts. Based on hybrid-seq (next-generation and single molecular real-time sequencing) of the S. miltiorrhiza root transcriptome, we experimentally identified 15 full-length transcripts and four alternative splicing events of enzyme-coding genes involved in the biosynthesis of rosmarinic acid. Moreover, we herein demonstrate that lithospermic acid B accumulates in the phloem and xylem of roots, in agreement with the expression patterns of the identified key genes related to rosmarinic acid biosynthesis. According to co-expression patterns, we predicted that six candidate cytochrome P450s and five candidate laccases participate in the salvianolic acid pathway. Our results provide a valuable resource for further investigation into the synthetic biology of phenolic acids in S. miltiorrhiza.
authors.affiliation: Institute of Medicinal Plant Development - Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Institute of Medicinal Plant Development - Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Luo
authors.affiliation: Institute of Medicinal Plant Development - Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors.forename: Aijia
authors.initials: A
authors.lastname: Ji
authors.affiliation: Institute of Medicinal Plant Development - Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development - Chinese Academy of Medical Sciences, Peking Union Medical College Beijing, China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Song
authors.affiliation: Institute of Medicinal Plant Development - Chinese Academy of Medical Sciences, Peking Union Medical CollegeBeijing, China; Institute of Chinese Materia Medica - Chinese Academy of Chinese Medical ScienceBeijing, China.
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
keywords: Salvia miltiorrhiza; alternative splicing; cytochrome P450s; full-length transcripts; hybrid-seq; laccases; phenolic acid biosynthesis