title: Positron Emission Tomography and Autoradiography of (18)F-Fluorodeoxyglucose Labeled Islets With or Without Warm Ischemic Stress in Portal Transplanted Rats.
abstract: OBJECTIVE
  The use of positron-emission tomography (PET) with (18)F-fluorodeoxyglucose (FDG) -labeled islets has been considered to be a potential modality to visualize and quantify early engraftment of islet transplantation. The objective of this study was to evaluate the early islets' survival of the FDG-labeled islets with or without warm ischemic stress in portal transplanted rats using PET and autoradiography.
  
  
  METHODS
  Islets were isolated from Lewis rat pancreata with or without 30-minute warm ischemia times (WITs). For islets' labeling, 300 islets were incubated with 3 MBq FDG for 60 minutes. FDG-labeled islets were transplanted into the liver via portal vein. In in vivo study, a PET study was scanned for 90 minutes and the FDG uptake was expressed as percentage of liver injection dose (ID). In ex vivo study, the liver was exposed for 30 minutes with single fluorescence autoradiography.
  
  
  RESULTS
  In the PET study, the percentage of liver ID of the islets without WIT was 27.8 and that of the WIT islets was 20.1 at the end of islet transplantation. At 90 minutes after transplantation, the percentage of liver ID was decreased to 14.7 in the islets without WIT and 10.1 in the WIT islets. In the autoradiogram, the number of hot spots was more obviously visualized in the liver transplanted without WIT islets than in the liver transplanted with WIT islets.
  
  
  CONCLUSIONS
  Almost 50% of the islets were immediately lost in both the islets without WIT and those with WIT transplantation in the early period. However, islet survival was 1.4 times higher in the islets without WIT than that in those with WIT in the early engraftment phase.
authors.affiliation: Department of Surgery, National Chiba-East Hospital, Chiba, Japan. Electronic address: otsuki@cehpnet.com.
authors.forename: K
authors.initials: K
authors.lastname: Otsuki
authors.affiliation: Department of Transplantation Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.affiliation: Department of Transplantation Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kenmochi
authors.affiliation: Department of Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Maruyama
authors.affiliation: Department of Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Akutsu
authors.affiliation: Department of Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saigo
authors.affiliation: Department of Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.affiliation: Department of Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Aoyama
authors.affiliation: Department of Surgery, National Chiba-East Hospital, Chiba, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Matsumoto
authors.affiliation: Chiba Ryogo Center, PET Imaging Division, Chiba, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Uchino
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002470:Cell Survival; D019788:Fluorodeoxyglucose F18; D007515:Islets of Langerhans / Q000000981:diagnostic imaging* / Q000503:physiopathology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008099:Liver; D008297:Male; D011169:Portal Vein / Q000637:transplantation*; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals; D051381:Rats; D011917:Rats, Inbred Lew; D013194:Staining and Labeling; D019737:Transplants; D052096:Warm Ischemia / Q000009:adverse effects
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18