title: Challenges and Opportunities in the Development of Aptamers for TNFα.
abstract: RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not the only crucial parameter for efficacy and functionality of those aptamers. Instead, the sensitive equilibrium of the monomeric and homotrimeric form of soluble TNFα decides on aptamer binding. Our results show that the field of application and the source of TNFα have to be carefully defined before selection of aptamer sequences.
authors.affiliation: Institut für Biochemie, Ernst-Moritz-Arndt Universität Greifswald, Greifswald, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Nübel
authors.affiliation: Institut für Biochemie, Ernst-Moritz-Arndt Universität Greifswald, Greifswald, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Appel
authors.affiliation: Materials Science Laboratory, Sony Deutschland GmbH, Stuttgart, Germany.|Institute of Electronics and Sensor Materials, Technical University Freiberg, Freiberg, Germany.
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Hospach
authors.affiliation: Materials Science Laboratory, Sony Deutschland GmbH, Stuttgart, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Mai
authors.affiliation: Materials Science Laboratory, Sony Deutschland GmbH, Stuttgart, Germany.
authors.forename: Nadejda
authors.initials: N
authors.lastname: Krasteva
authors.affiliation: Materials Science Laboratory, Sony Deutschland GmbH, Stuttgart, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Nelles
authors.affiliation: Interfakultäres Institut für Genetik und funktionelle Genomforschung, Ernst-Moritz-Arndt Universität Greifswald, Greifswald, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Petruschka
authors.affiliation: Institut für Biochemie, Ernst-Moritz-Arndt Universität Greifswald, Greifswald, Germany. smueller@uni-greifswald.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Müller
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques; D006801:Humans; D058525:Quartz Crystal Microbalance Techniques; D020349:Surface Plasmon Resonance; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D052157:Aptamers, Nucleotide; D014409:Tumor Necrosis Factor-alpha
keywords: Aptamer; Biotin linker; Cytokine; ELISA; In vitro selection; QCM; Rational design; SPR; TNFα