title: Mechanism of Protein Molecule Isolation by IR Laser Ablation of Droplet Beam.
abstract: Gas-phase isolation of bovine serum albumin (BSA) from aqueous solutions is performed by IR laser ablation of a droplet beam. Multiply charged BSA ions (positive and negative) were produced by the IR laser irradiation onto a droplet beam of aqueous BSA solutions with various pH values prepared by addition of hydrochloric acid or sodium hydroxide to the solution. The isolation mechanism was discussed based on the charge state of the isolated BSA ions. A nanodroplet model explains the gas-phase charge distribution of the BSA ions. This study provides a fundamental basis for further studies of a wide variety of biomolecules in the gas phase isolated directly from solution.
authors.affiliation: Department of Chemistry, Faculty of Science, Gakushuin University , 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Komatsu
authors.affiliation: Department of Chemistry, Faculty of Science, Gakushuin University , 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Nirasawa
authors.affiliation: Department of Chemistry, Faculty of Science, Gakushuin University , 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Hoshino-Nagasaka
authors.affiliation: Department of Chemistry, Faculty of Science, Gakushuin University , 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan.
authors.forename: Jun-ya
authors.initials: JY
authors.lastname: Kohno
mesh_terms: D007259:Infrared Rays; D007834:Lasers; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine