title: Stenotrophomonas maltophilia biofilm reduction by Bdellovibrio exovorus.
abstract: Stenotrophomonas maltophilia, a bacterium ubiquitous in the environment, is also an opportunistic, multidrug-resistant human pathogen that colonizes tissues and medical devices via biofilm formation. We investigated the ability of an isolate from sewage of the bacterial predator Bdellovibrio exovorus to disrupt preformed biofilms of 18 strains of S. maltophilia isolated from patients, hospital sink drains and water fountain drains. B. exovorus FFRS-5 preyed on all S. maltophilia strains in liquid co-cultures and was able to significantly disrupt the biofilms of 15 of the S. maltophilia strains tested, decreasing as much as 76.7% of the biofilm mass. The addition of ciprofloxacin and kanamycin in general reduced S. maltophilia biofilms but less than that of B. exovorus alone. Furthermore, when antibiotics and B. exovorus were used together, B. exovorus was still effective in the presence of ciprofloxacin whereas the addition of kanamycin reduced the effectiveness of B. exovorus. Overall, B. exovorus was able to decrease the mass of preformed biofilms of S. maltophilia in the presence of clinically relevant antibiotics demonstrating that the predator may prove to be a beneficial tool to reduce S. maltophilia environmental or clinically associated biofilms.
authors.affiliation: Department of Microbiology and Immunology, University of Western Ontario, London, ON, Canada.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Chanyi
authors.affiliation: Department of Microbiology and Immunology, University of Western Ontario, London, ON, Canada.
authors.forename: Susan F
authors.initials: SF
authors.lastname: Koval
authors.affiliation: Department of Biological Sciences, College of Science and Health, DePaul University, Chicago, IL, USA.
authors.forename: Joanna S
authors.initials: JS
authors.lastname: Brooke
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001501:Bdellovibrio / Q000254:growth & development* / Q000302:isolation & purification; D018441:Biofilms / Q000254:growth & development*; D002939:Ciprofloxacin / Q000494:pharmacology; D004783:Environmental Microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D007612:Kanamycin / Q000494:pharmacology; D012722:Sewage / Q000382:microbiology; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D012722:Sewage; D007612:Kanamycin; D002939:Ciprofloxacin