title: Structural and dynamic views of the CRISPR-Cas system at the single-molecule level.
abstract: The CRISPR-Cas system has emerged as a fascinating and important genome editing tool. It is now widely used in biology, biotechnology, and biomedical research in both academic and industrial settings. To improve the specificity and efficiency of Cas nucleases and to extend the applications of these systems for other areas of research, an understanding of their precise working mechanisms is crucial. In this review, we summarize current studies on the molecular structures and dynamic functions of type I and type II Cas nucleases, with a focus on target DNA searching and cleavage processes as revealed by single-molecule observations. [BMB Reports 2016; 49(4): 201-207].
authors.affiliation: Center for Genome Engineering, Institute for Basic Science, Seoul 08826, Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Chemistry, and Institute for Materials Design, Hanyang University, Seoul 04763, Korea.
authors.forename: Sangsu
authors.initials: S
authors.lastname: Bae
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D064113:CRISPR-Cas Systems; D004247:DNA / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000378:metabolism
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems; D000255:Adenosine Triphosphate; D004247:DNA