title: [AN EVALUATION OF SPONTANEOUS HISTAMINE RELEASE AND THE LOW RESPONDERS IN A BASOPHIL HISTAMINE RELEASE TEST].
abstract: BACKGROUND
  We evaluated the clinical significance of the spontaneous histamine release ratio (SHR/T) and low responders in the automated basophil histamine release test (AllerportⓇ HRT).
  
  
  METHODS
  This study analyzed the outcomes of 101 oral food challenges (OFC) with egg, milk or wheat (challenge-positive: n=79) in relation to the SHR/T. The traditional HRT low responders (n=27) were separated into two groups:"LOW"responders (n=10), who showed a ≥10% concentration-dependent maximum histamine release in response to the anti-human IgE stimulation, and"NON"responders who did not fulfill the criteria (n=17).
  
  
  RESULTS
  Among the 34 patients with ≥20% SHR/T, 32 patients (94%) had a positive OFC with a low threshold dose which provoked severe symptoms. Among the"LOW"responders, four cases showed ≥10% allergen-specific maximum histamine release. On the other hand, concentration-dependent histamine release was not seen in the"NON"responders, suggesting the basophil function was not detected in this subgroup.
  
  
  CONCLUSIONS
  The present study suggested that SHR/T could be an indicator of basophil activation and hypersensitivity in vivo. We also suggested that significant basophil functions might be detected among the "LOW"responders, but not among the"NON"responders.
authors.affiliation: Department of Allergy, Aichi Children's Health and Medical Center.
authors.forename: Komei
authors.initials: K
authors.lastname: Ito
authors.forename: Sakura
authors.initials: S
authors.lastname: Sato
authors.forename: Atsuo
authors.initials: A
authors.lastname: Urisu
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Kando
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Ikuya
authors.initials: I
authors.lastname: Tsuge
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Kondo
authors.forename: Setsuko
authors.initials: S
authors.lastname: Ito
authors.forename: Masahito
authors.initials: M
authors.lastname: Higashiura
authors.forename: Motohiro
authors.initials: M
authors.lastname: Ebisawa
mesh_terms: D001491:Basophils / Q000276:immunology* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D006632:Histamine / Q000276:immunology*; D006636:Histamine Release; D006801:Humans; D007223:Infant; D008297:Male
chemical_list: D006632:Histamine