title: Age-related presence of selected viral and bacterial pathogens in paraffin-embedded lung samples of dogs with pneumonia.
abstract: The aim of this retrospective study was to detect selected pathogens in pneumonic lung tissue of dogs of different age groups by immunohistochemistry (IHC), in situ hybridisation (ISH) or polymerase chain reaction (PCR) in order to get information about their involvement in pneumonia formation. In archived formalin-fixed and paraffin wax-embedded lung samples from 68 cases with the clinical and histologic diagnosis of pneumonia the histological pattern of pneumonia was re-evaluated and the samples were further investigated for the following infectious agents: canine distemper virus (CDV), canine adenovirus type 2 (CAV-2), canine respiratory coronavirus (CRCoV), Bordetella (B.) bronchiseptica, Pasteurella (P.) multocida, Mycoplasma spp., and Pneumocystis spp. In 47.1% of the samples at least one of the featured respiratory pathogens was detected. In 31.3% of these positive samples more than one pathogen could be found. The correct detection of CDV had been achieved in ten out of eleven positive cases (90.9%) upon initial investigation, but the presence of bacterial pathogens, like B. bronchiseptica (10 cases) and P. multocida (17 cases) had been missed in all but one case. While CDV and CRCoV infections were exclusively found in dogs younger than one year, the vast majority of infections with P. multocida and B. bronchiseptica were both common either in dogs younger than 4 months or older than one year. Thus, this retrospective approach yielded valuable data on the presence, absence and prevalence of certain respiratory pathogens in dogs with pneumonia.
authors.affiliation: Institute of Pathology and Forensic Veterinary Medicine, University of Veterinary Medicine Vienna , Veterinärplatz 1, A-1210 Vienna , Austria.
authors.forename: Daniela
authors.initials: D
authors.lastname: Wöhrer
authors.affiliation: Institute of Microbiology, University of Veterinary Medicine Vienna , Veterinärplatz 1, A-1210 Vienna , Austria.
authors.forename: Joachim
authors.initials: J
authors.lastname: Spergser
authors.affiliation: Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna , Veterinärplatz 1, A-1210 Vienna , Austria.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bagrinovschi
authors.affiliation: Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna , Veterinärplatz 1, A-1210 Vienna , Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Möstl
authors.affiliation: Institute of Pathology and Forensic Veterinary Medicine, University of Veterinary Medicine Vienna , Veterinärplatz 1, A-1210 Vienna , Austria.
authors.forename: Herbert
authors.initials: H
authors.lastname: Weissenböck
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D004283:Dog Diseases / Q000382:microbiology / Q000473:pathology; D004285:Dogs; D007150:Immunohistochemistry / Q000662:veterinary; D017403:In Situ Hybridization / Q000662:veterinary; D008168:Lung / Q000382:microbiology* / Q000473:pathology*; D016612:Paraffin Embedding; D011014:Pneumonia / Q000662:veterinary*; D012189:Retrospective Studies; D014780:Viruses / Q000302:isolation & purification*
keywords: Dog; pneumonia; respiratory disease; respiratory pathogens; retrospective study