title: Ketide Synthase (KS) Domain Prediction and Analysis of Iterative Type II PKS Gene in Marine Sponge-Associated Actinobacteria Producing Biosurfactants and Antimicrobial Agents.
abstract: The important biological macromolecules, such as lipopeptide and glycolipid biosurfactant producing marine actinobacteria were analyzed and their potential linkage between type II polyketide synthase (PKS) genes was explored. A unique feature of type II PKS genes is their high amino acid (AA) sequence homology and conserved gene organization. These enzymes mediate the biosynthesis of polyketide natural products with enormous structural complexity and chemical nature by combinatorial use of various domains. Therefore, deciphering the order of AA sequence encoded by PKS domains tailored the chemical structure of polyketide analogs still remains a great challenge. The present work deals with an in vitro and in silico analysis of PKS type II genes from five actinobacterial species to correlate KS domain architecture and structural features. Our present analysis reveals the unique protein domain organization of iterative type II PKS and KS domain of marine actinobacteria. The findings of this study would have implications in metabolic pathway reconstruction and design of semi-synthetic genomes to achieve rational design of novel natural products.
authors.affiliation: Department of Microbiology, Pondicherry UniversityKalapet, India; Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Sciences, King Saud UniversityRiyadh, Saudi Arabia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Selvin
authors.affiliation: School of Life Sciences, Bharathidasan University Tiruchirappalli, India.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Sathiyanarayanan
authors.affiliation: Department of Microbiology, Pondicherry UniversityKalapet, India; Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Sciences, King Saud UniversityRiyadh, Saudi Arabia.
authors.forename: Anuj N
authors.initials: AN
authors.lastname: Lipton
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Sciences, King Saud University Riyadh, Saudi Arabia.
authors.forename: Naif Abdullah
authors.initials: NA
authors.lastname: Al-Dhabi
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Sciences, King Saud University Riyadh, Saudi Arabia.
authors.forename: Mariadhas
authors.initials: M
authors.lastname: Valan Arasu
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Sciences, King Saud UniversityRiyadh, Saudi Arabia; Department of Food Science and Technology, Pondicherry UniversityKalapet, India.
authors.forename: George S
authors.initials: GS
authors.lastname: Kiran
keywords: actinobacteria; biosurfactant; glycolipid; lipopeptide; polyketide synthases; three-dimensional structure