title: Complete Nucleotide Sequence of pKOI-34, an IncL/M Plasmid Carrying blaIMP-34 in Klebsiella oxytoca Isolated in Japan.
abstract: We determined the complete nucleotide sequence of a self-transmissible IncL/M plasmid, pKOI-34, from a Klebsiella oxytoca isolate. pKOI-34 possessed the core structure of an IncL/M plasmid found in Erwinia amylovora, pEL60, with two mobile elements inserted, a transposon carrying the arsenic resistance operon and a Tn21-like core module (tnp and mer modules) piggybacking blaIMP-34 as a class 1 integron, In808, where blaIMP-34 confers a resistance to carbapenems in K. oxytoca and Klebsiella pneumoniae.
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Surgery I, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Norimitsu
authors.initials: N
authors.lastname: Shimada
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Kayama
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Infectious Diseases, Hiroshima University Hospital, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Shigemoto
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Junzo
authors.initials: J
authors.lastname: Hisatsune
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Clinical Laboratory, Hiroshima General Hospital of West Japan Railway Company, Futaba-no-sato 3-1-36, Higashi-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Kuwahara
authors.affiliation: Clinical Laboratory, Shiga Medical Center for Adults, 5-4-30, Moriyama, Moriyama City, Shiga, Japan.
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Nishio
authors.affiliation: Department of Clinical Laboratory, Wakayama Rosai Hospital, 93-1 Kinomoto, Wakayama City, Wakayama, Japan.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yamasaki
authors.affiliation: Clinical Laboratory, Hyogo Medical University Hospital, 1-1 Mukogawa-cho, Nishinomiya City, Hyogo, Japan.
authors.forename: Yasunao
authors.initials: Y
authors.lastname: Wada
authors.affiliation: Department of Surgery I, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Taijiro
authors.initials: T
authors.lastname: Sueda
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Infectious Diseases, Hiroshima University Hospital, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohge
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima, Japan sugai@hiroshima-u.ac.jp.
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Sugai
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D007564:Japan; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements