title: Effect of different polyphenol sources on the efficiency of ellagic acid release by Aspergillus niger.
abstract: Fungal hydrolysis of ellagitannins produces hexahydroxydiphenic acid, which is considered an intermediate molecule in ellagic acid release. Ellagic acid has important and desirable beneficial health properties. The aim of this work was to identify the effect of different sources of ellagitannins on the efficiency of ellagic acid release by Aspergillus niger. Three strains of A. niger (GH1, PSH and HT4) were assessed for ellagic acid release from different polyphenol sources: cranberry, creosote bush, and pomegranate used as substrate. Polyurethane foam was used as support for solid-state culture in column reactors. Ellagitannase activity was measured for each of the treatments. Ellagic acid was quantified by high performance liquid chromatography. When pomegranate polyphenols were used, a maximum value of ellagic acid (350.21 mg/g) was reached with A. niger HT4 in solid-state culture. The highest amount of ellagitannase (5176.81 U/l) was obtained at 8h of culture when cranberry polyphenols and strain A. niger PSH were used. Results demonstrated the effect of different polyphenol sources and A. niger strains on ellagic acid release. It was observed that the best source for releasing ellagic acid was pomegranate polyphenols and A. niger HT4 strain, which has the ability to degrade these compounds for obtaining a potent bioactive molecule such as ellagic acid.
authors.affiliation: Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sepúlveda
authors.affiliation: Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico.
authors.forename: Reynaldo
authors.initials: R
authors.lastname: de la Cruz
authors.affiliation: Department of Biotechnology, Departamento de Biotecnología, Universidad Autónoma Metropolitana, 09340 Iztapalapa, Mexico.
authors.forename: José Juan
authors.initials: JJ
authors.lastname: Buenrostro
authors.affiliation: Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico.
authors.forename: Juan Alberto
authors.initials: JA
authors.lastname: Ascacio-Valdés
authors.affiliation: Animal Nutrition Department, Universidad Autónoma Agraria Antonio Narro, 25315, Buenavista, Saltillo, Coahuila, Mexico.
authors.forename: Antonio Francisco
authors.initials: AF
authors.lastname: Aguilera-Carbó
authors.affiliation: Department of Biotechnology, Departamento de Biotecnología, Universidad Autónoma Metropolitana, 09340 Iztapalapa, Mexico.
authors.forename: Arely
authors.initials: A
authors.lastname: Prado
authors.affiliation: Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico.
authors.forename: Raúl
authors.initials: R
authors.lastname: Rodríguez-Herrera
authors.affiliation: Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, 25280 Saltillo, Coahuila, Mexico. Electronic address: cristobal.aguilar@uadec.edu.mx.
authors.forename: Cristóbal Noé
authors.initials: CN
authors.lastname: Aguilar
mesh_terms: D001234:Aspergillus niger / Q000187:drug effects* / Q000378:metabolism*; D004610:Ellagic Acid / Q000378:metabolism*; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D032442:Larrea; D029561:Lythraceae; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D029799:Vaccinium macrocarpon
chemical_list: D047348:Hydrolyzable Tannins; D010936:Plant Extracts; D059808:Polyphenols; D004610:Ellagic Acid
keywords: Aspergillus niger; Cultivo en estado sólido; Elagitanasa; Ellagitannase; Polifenoles; Polyphenols; Solid-state culture