title: Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples.
abstract: To evaluate the clinical efficacy of a procedure comprising a combination of Percoll continuous density gradient and modified swim-up techniques for the removal of human immunodeficiency virus type 1 (HIV-1) from the semen of HIV-1 infected males, a total of 129 couples with an HIV-1 positive male partner and an HIV-1 negative female partner (serodiscordant couples) who were treated at Keio University Hospital between January 2002 and April 2012 were examined. A total of 183 ejaculates from 129 HIV-1 infected males were processed. After swim-up, we successfully collected motile sperms at a recovery rate as high as 100.0% in cases of normozoospermia (126/126 ejaculates), oligozoospermia (6/6), and asthenozoospermia (36/36). The recovery rate of oligoasthenozoospermia was 86.7% (13/15). In processed semen only four ejaculates (4/181:2.2%) showed viral nucleotide sequences consistent with those in the blood of the infected males. After using these sperms, no horizontal infections of the female patients and no vertical infections of the newborns were observed. Furthermore, no obvious adverse effects were observed in the offspring. This protocol allowed us to collect HIV-1 negative motile sperms at a high rate, even in male factor cases. We concluded that our protocol is clinically effective both for decreasing HIV-1 infections and for yielding a healthy child.
authors.affiliation: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 1608582, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Inoue
authors.affiliation: Department of Obstetrics and Gynecology, Tokyo Medical College, Tokyo 1600023, Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Kuji
authors.affiliation: Department of Obstetrics and Gynecology, Tokyo Medical College, Tokyo 1600023, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Ito
authors.affiliation: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 1608582, Japan.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 1608582, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Hamatani
authors.affiliation: Department of Microbiology, Keio University School of Medicine, Tokyo 1608582, Japan.
authors.forename: Aimi
authors.initials: A
authors.lastname: Oyadomari
authors.affiliation: Department of Microbiology, Keio University School of Medicine, Tokyo 1608582, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kato
authors.affiliation: Department of Hematology, Ogikubo Hospital, Tokyo 1670035, Japan.
authors.forename: Hideji
authors.initials: H
authors.lastname: Hanabusa
authors.affiliation: Department of Obstetrics and Gynecology, Tokyo Medical College, Tokyo 1600023, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Isaka
authors.affiliation: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 1608582, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D000328:Adult; D002499:Centrifugation, Density Gradient; D005260:Female; D015658:HIV Infections / Q000517:prevention & control*; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011205:Povidone / Q000627:therapeutic use*; D011247:Pregnancy; D018873:Pregnancy Rate; D012367:RNA, Viral / Q000235:genetics; D027724:Reproductive Techniques, Assisted; D012661:Semen; D012662:Semen Preservation; D012822:Silicon Dioxide / Q000627:therapeutic use*; D013094:Spermatozoa / Q000821:virology*; D016896:Treatment Outcome
chemical_list: D012367:RNA, Viral; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone