title: Immunohistochemical distribution of the cannabinoid receptor 1 and fatty acid amide hydrolase in the dog claustrum.
abstract: Cannabinoid receptor 1 (CB1R) and fatty acid amide hydrolase (FAAH) are part of the endocannabinoid system (ECB) which exerts a neuromodulatory activity on different brain functions and plays a key role in neurogenesis. Although many studies have reported FAAH and CB1R expression in the brain of different animal species, to the best of our knowledge they have never been described in the canine claustrum. Claustrum samples, obtained from necropsy of four neurologically normal dogs, were formalin fixed for paraffin embedding. Sections were either stained for morpho-histological analysis or immunostained for CB1R and FAAH. Analysis of adjacent sections incubated with the two antisera showed a complementary labeling pattern in the claustrum, with CB1R antibody staining fibers while anti-FAAH antibody stained cell bodies and the proximal portion of dendrites; this particular anatomical relationship suggests a retrograde endocannabinoid action via CB1R. CB1R and FAAH complementary immunostaining and their cellular localization reported here provide the first anatomical evidence for existence of the ECB in the dog claustrum.
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Pisa, Italy. Electronic address: andrea.pirone@unipi.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Pirone
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Pisa, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Cantile
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Pisa, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Miragliotta
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Pisa, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Lenzi
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Pisa, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Giannessi
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Cozzi
mesh_terms: D000581:Amidohydrolases / Q000032:analysis*; D000818:Animals; D001479:Basal Ganglia / Q000737:chemistry* / Q000473:pathology; D004285:Dogs; D005260:Female; D008297:Male; D043884:Receptor, Cannabinoid, CB1 / Q000032:analysis*; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D043884:Receptor, Cannabinoid, CB1; D000099252:Fatty Acid Amide Hydrolases
keywords: CB1R; Cannabinoid; Claustrum; Dog; FAAH; Immunohistochemistry