title: Prevention of osteoradionecrosis of the jaws by low-intensity ultrasound in the dog model.
abstract: The prevention of osteoradionecrosis of the jaws (ORNJ) is very important because of the current absence of effective therapies for this disease. The aim of this study was to determine whether low-intensity ultrasound has a preventive effect on ORNJ. Sixty healthy adult dogs were divided randomly into three groups: group A (radiotherapy alone), group B (radiotherapy followed by low-intensity ultrasound treatment), and a control group. The development of ORNJ was assessed and the rate of occurrence of ORNJ was compared between groups A and B. Micro-computed tomography, haematoxylin-eosin staining, and immunofluorescence were used to evaluate the microstructure of the mandible and changes in microvascular density in all groups. All animals in group A and group B (ultrasound applied for 30 days) developed ORNJ. Alveolar bone density was 609.48±53.77HU in group A and 829.65±81.46HU in group B (P=0.008). The trabecular bone volume fraction, bone surface area/bone volume ratio, trabecular thickness, and trabecular number were all lower in group A than in group B (P=0.037, P=0.022, P=0.017, and P=0.034, respectively). Haematoxylin-eosin staining showed that the Haversian canals in the osteons had expanded significantly in group A, with collagen fibres losing their circular orientation; group B tended to show typical osteons. The microvascular density in group A was decreased. In conclusion, the use of low-intensity ultrasound in the dog appears not to prevent the incidence of ORNJ, however it does somewhat improve vascularity and bone quality at the microscopic level, which contribute to ORNJ healing.
authors.affiliation: The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: M
authors.initials: M
authors.lastname: Lang
authors.affiliation: Wenzhou Central Hospital, Wenzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Fan
authors.affiliation: Wenzhou Integration Traditional Chinese and Western Medicine Hospital, Wenzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.affiliation: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Xiao
authors.affiliation: The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, China. Electronic address: zzh814490194@126.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D007568:Jaw / Q000000981:diagnostic imaging / Q000528:radiation effects*; D007571:Jaw Diseases / Q000517:prevention & control*; D008297:Male; D010025:Osteoradionecrosis / Q000517:prevention & control*; D011897:Random Allocation; D014464:Ultrasonic Therapy / Q000379:methods*; D055114:X-Ray Microtomography
keywords: head and neck cancer; hyperbaric oxygen; low-intensity ultrasound; osteoradionecrosis; prevention; radiotherapy