title: Small bowel in vivo bioengineering using an aortic matrix in a porcine model.
abstract: To evaluate the feasibility of an in vivo small bowel bioengineering model using allogeneic aortic grafts in pigs.
  
  
  
  The best treatment for short bowel syndrome is still unclear. Intestinal transplantation, as well as lifelong parenteral nutrition is associated with a 5-year survival rate of less than 50 %. We have already used allogeneic arterial segments to replace the upper airway in sheep. The results were encouraging with an induced transformation of the aortic wall into tracheo-bronchial bronchial-type tissue.
  
  
  
  Seven young mini-pigs were used. A 10-cm-diameter, allogeneic, aortic graft was interposed in an excluded small bowel segment and wrapped by the neighboring omentum. Animals were autopsied at 1 (n = 2), 3 (n = 3), and 6 months (n = 2), respectively. Specimens were examined macroscopically and microscopically.
  
  
  
  The overall survival rate of the animals was 71.4 %. No anastomotic leak occurred. Histologic analysis revealed intestinal-like wall transformation of the aortic graft in the surviving animals.
  
  
  
  Aortic-enteric anastomosis is feasible in a porcine model. Moreover, in vivo, bioengineered, intestinal-like transformation of the vascular wall was identified.
authors.affiliation: Department of General and Minimally Invasive Surgery, Centre Hospitalier de Poissy/Saint-Germain-En-Laye, 10, rue du Champ Gaillard, 78300, Poissy, France. chouillard@yahoo.com.
authors.forename: Elie
authors.initials: E
authors.lastname: Chouillard
authors.affiliation: Department of General and Minimally Invasive Surgery, Centre Hospitalier de Poissy/Saint-Germain-En-Laye, 10, rue du Champ Gaillard, 78300, Poissy, France.
authors.forename: Elias
authors.initials: E
authors.lastname: Chahine
authors.affiliation: Centre de Recherche Chirurgicale Dominique Chopin, Hôpital Henri Mondor, UPEC, Créteil, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Allaire
authors.affiliation: Centre de Recherche Chirurgicale Dominique Chopin, Hôpital Henri Mondor, UPEC, Créteil, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Filaire-Legendre
authors.affiliation: Centre de Recherche Chirurgicale Dominique Chopin, Hôpital Henri Mondor, UPEC, Créteil, France.
authors.forename: Jeanne Tran
authors.initials: JT
authors.lastname: Van Nhieu
authors.affiliation: Department of Thoracic and Vascular Surgery, Faculty of Medecine, SMBH Bobigny, Assistance Publique-Hôpitaux de Paris, Hôpitaux Universiatires Paris Seine-Saint-Deanis, Avicenne Hospital, Paris 13 University, Sorbonne-Paris Cité, Bobigny, France.|Alain Carpentier Foundation, Laboratory for Biosurgical Research, Georges Pompidou European Hospital, Paris Descartes University, Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Martinod
mesh_terms: D064591:Allografts; D000818:Animals; D001011:Aorta / Q000637:transplantation*; D057005:Bioengineering / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D005260:Female; D007421:Intestine, Small / Q000601:surgery* / Q000637:transplantation; D007422:Intestines; D010289:Parenteral Nutrition, Total; D010865:Pilot Projects; D012778:Short Bowel Syndrome / Q000601:surgery; D013552:Swine; D013556:Swine, Miniature
keywords: Animal; Aorta; Bioengineering; Bowel; Engineering; Graft; Regenerative; Surgery; Transplantation