title: Genome sequence of Escherichia coli NCCP15653, a group D strain isolated from a diarrhea patient.
abstract: BACKGROUND
  Pathogenic strains in Escherichia coli can be divided into several pathotypes according to their virulence features. Among them, uropathogenic E. coli causes most of the urinary tract infections and has a genotype distinct from other virulent strains of E. coli. In this study, we sequenced and analyzed the genome of E. coli NCCP15653 isolated from the feces of a diarrhea patient in 2007 in South Korea.
  
  
  RESULTS
  A phylogenetic tree based on MLST showed that NCCP15653 belongs to the D group of E. coli and located in the lineage containing strains ST2747, UMN026 and 042. In the genome of NCCP15653, genes encoding major virulence factors of uropathogenic E. coli were detected. They include type I fimbriae, hemin uptake proteins, iron/manganese transport proteins, yersiniabactin siderophore proteins, type VI secretion proteins, and hemolysin. On the other hand, genes encoding AslA, OmpA, and the K1 capsule, which are virulence factors associated with invasion of neonatal meningitis-causing E. coli, were also present, while a gene encoding CNF-1 protein, which is a cytotoxic necrotizing factor 1, was not detected.
  
  
  CONCLUSIONS
  Through the genome analysis of NCCP15653, we report an example of a genome of chimeric pathogenic properties. The gene content of NCCP15653, a group D strain, demonstrates that it could be both uropathogenic E. coli and neonatal meningitis-causing E. coli. Our results suggest the dynamic nature of plastic genomes in pathogenic strains of E. coli.
authors.affiliation: Department of Systems Biology and Division of Life Sciences, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749 Republic of Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kwak
authors.affiliation: Department of Systems Biology and Division of Life Sciences, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749 Republic of Korea.
authors.forename: Myung-Soo
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Department of Systems Biology and Division of Life Sciences, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749 Republic of Korea.
authors.forename: Soon-Kyeong
authors.initials: SK
authors.lastname: Kwon
authors.affiliation: Division of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of Health, Heungdeok-Gu, Cheongju, 363-951 Republic of Korea.
authors.forename: Seung-Hak
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Systems Biology and Division of Life Sciences, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749 Republic of Korea.
authors.forename: Jihyun F
authors.identifier: 0000-0001-7715-6992
authors.initials: JF
authors.lastname: Kim
keywords: Cystitis; Extraintestinal E. coli; NMEC; Pyelonephritis; UPEC