title: Time related variations in stem cell harvesting of umbilical cord blood.
abstract: Umbilical cord blood (UCB) contains hematopoietic stem cells and multipotent mesenchymal cells useful for treatment in malignant/nonmalignant hematologic-immunologic diseases and regenerative medicine. Transplantation outcome is correlated with cord blood volume (CBV), number of total nucleated cells (TNC), CD34+ progenitor cells and colony forming units in UCB donations. Several studies have addressed the role of maternal/neonatal factors associated with the hematopoietic reconstruction potential of UCB, including: gestational age, maternal parity, newborn sex and birth weight, placental weight, labor duration and mode of delivery. Few data exist regarding as to how time influences UCB collection and banking patterns. We retrospectively analyzed 17.936 cord blood donations collected from 1999 to 2011 from Tuscany and Apulia Cord Blood Banks. Results from generalized multivariable linear mixed models showed that CBV, TNC and CD34+ cell were associated with known obstetric and neonatal parameters and showed rhythmic patterns in different time domains and frequency ranges. The present findings confirm that volume, total nucleated cells and stem cells of the UCB donations are hallmarked by rhythmic patterns in different time domains and frequency ranges and suggest that temporal rhythms in addition to known obstetric and neonatal parameters influence CBV, TNC and CD34+ cell content in UBC units.
authors.affiliation: Department of Medical Sciences, Division of Internal Medicine and Chronobiology Unit, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Mazzoccoli
authors.affiliation: Apulia Cord Blood Bank, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Miscio
authors.affiliation: Unit of Biostatistics, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fontana
authors.affiliation: Unit of Biostatistics, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Copetti
authors.affiliation: Computing Unit, IRCCS Scientific Institute and Regional General Hospital "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Francavilla
authors.affiliation: Department of Clinical and Experimental Medicine, Unit of Haematology, School of Medicine, University of Florence, Florence, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Bosi
authors.affiliation: Department of Clinical and Experimental Medicine, School of Medicine, University of Florence, Florence, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Perfetto
authors.affiliation: Interinstitutional Department for Continuity of Care of Empoli, School of Medicine, University of Florence, Florence, Italy.
authors.forename: Alice
authors.initials: A
authors.lastname: Valoriani
authors.affiliation: Department of Medical Sciences, Division of Internal Medicine and Chronobiology Unit, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Angelo
authors.initials: A
authors.lastname: De Cata
authors.affiliation: Apulia Cord Blood Bank, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Santodirocco
authors.affiliation: Apulia Cord Blood Bank, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Totaro
authors.affiliation: Department of Medical Sciences, Division of Internal Medicine and Chronobiology Unit, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Rubino
authors.affiliation: Apulia Cord Blood Bank, IRCCS "Casa Sollievo della Sofferenza", S.Giovanni Rotondo (FG), Italy.
authors.forename: Lazzaro
authors.initials: L
authors.lastname: di Mauro
authors.affiliation: Interinstitutional Department for Continuity of Care of Empoli, School of Medicine, University of Florence, Florence, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Tarquini
mesh_terms: D000293:Adolescent; D000328:Adult; D001724:Birth Weight; D001800:Blood Specimen Collection / Q000379:methods*; D002469:Cell Separation; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008423:Maternal Age; D008875:Middle Aged; D012189:Retrospective Studies; D013997:Time Factors; D055815:Young Adult