title: Medium-chain fatty acid reduces lipid accumulation by regulating expression of lipid-sensing genes in human liver cells with steatosis.
abstract: Accumulation of lipids in the liver can lead to cell dysfunction and steatosis, an important factor in pathogenesis causing non-alcoholic fatty liver disease. The mechanisms related to lipid deposition in the liver, however, remain poorly understood. This study was aimed to investigate the effects of medium-chain fatty acid (MCFA) on the lipolysis and expression of lipid-sensing genes in human liver cells with steatosis. A cellular steatosis model, which is suitable to experimentally investigate the impact of fat accumulation in the liver, was established in human normal liver cells (LO2 cells) with a mixture of free fatty acids (oleate/palmitate, 2:1) at 200 μm for 24 h incubation. MCFA was found to down-regulate expression of liver X receptor-α, sterol regulatory element binding protein-1, acetyl-CoA carboxylase, fatty acid synthase, CD 36 and lipoprotein lipase in this cellular model, and have positive effects on adipose triglyceride lipase and hormone-sensitive lipase. These results suggest that MCFA may reduce lipid accumulation by regulating key lipid-sensing genes in human liver cells with steatosis.
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China ;
authors.forename: Baogui
authors.initials: B
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China ;
authors.forename: Jing
authors.initials: J
authors.lastname: Fu
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China ;
authors.forename: Lumin
authors.initials: L
authors.lastname: Li
authors.affiliation: b School of Biological Sciences , The University of Auckland , Auckland , New Zealand ;
authors.forename: Deming
authors.initials: D
authors.lastname: Gong
authors.affiliation: c School of Resource and Environmental and Chemical Engineering , Nanchang University , Nanchang , China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Wen
authors.affiliation: c School of Resource and Environmental and Chemical Engineering , Nanchang University , Nanchang , China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China ;|c School of Resource and Environmental and Chemical Engineering , Nanchang University , Nanchang , China.
authors.forename: Zheling
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D005227:Fatty Acids / Q000737:chemistry* / Q000494:pharmacology*; D005234:Fatty Liver / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000502:physiology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D012333:RNA, Messenger
keywords: Lipolysis; liver X receptor-α; medium-chain fatty acid; non-alcoholic fatty liver disease; sterol regulatory element binding protein-1