title: Effect of Hypercholesterolemia on Fatty Infiltration and Quality of Tendon-to-Bone Healing in a Rabbit Model of a Chronic Rotator Cuff Tear: Electrophysiological, Biomechanical, and Histological Analyses.
abstract: The incidence of healing failure after rotator cuff repair is high, and fatty infiltration is a crucial factor in healing failure.
  
  
  
  To verify the effect of hypercholesterolemia on fatty infiltration and the quality of tendon-to-bone healing and its reversibility by lowering the cholesterol level in a chronic tear model using the rabbit supraspinatus.
  
  
  
  Controlled laboratory study.
  
  
  
  Forty-eight rabbits were randomly allocated into 4 groups (n = 12 each). After 4 weeks of a high-cholesterol diet (groups A and B) and a regular diet (groups C and D), the supraspinatus tendon was detached and left alone for 6 weeks and then was repaired in a transosseous manner (groups A, B, and C). Group D served as a control. Group A continued to receive the high-cholesterol diet until the final evaluation (6 weeks after repair); however, at the time of repair, group B was changed to a general diet with administration of a cholesterol-lowering agent (simvastatin). Histological evaluation of the fat-to-muscle proportion was performed twice, at the time of repair and the final evaluation, and an electromyographic (EMG) test, mechanical test, and histological test of tendon-to-bone healing were performed at the final evaluation.
  
  
  
  For the EMG test, group A showed a significantly smaller area of compound muscle action potential compared with groups C and D (all P <.01), and group B showed a larger area than group A, almost up to the level of group C (P = .312). Similarly, group A showed significantly lower mechanical properties both in load-to-failure and stiffness compared with groups C and D (all P <.05). In addition, although not significantly different, the mechanical properties of group B were higher than those of group A (mean load-to-failure: group A = 42.01 N, group B = 58.23 N [P = .103]; mean stiffness: group A = 36.32 N/mm, group B = 47.22 N/mm [P = .153]). For the histological test, groups A and B showed a significantly higher fat-to-muscle proportion than did groups C and D at 6 weeks after detachment (all P <.05), but at the final evaluation, group B showed a decreased fat-to-muscle proportion (mean ± SD: from 64.02% ± 11.87% to 54.68% ± 10.47%; P = .146) compared with group A, which showed increased fat-to-muscle proportion (from 59.26% ± 17.80% to 78.23% ± 10.87%; P = .015). Groups B and C showed better tendon-to-bone interface structures than did group A, which showed coarse and poorly organized collagen fibers with fat interposition.
  
  
  
  Hypercholesterolemia had a deleterious effect on fatty infiltration and the quality of tendon-to-bone repair site, and lowering hypercholesterolemia seemed to halt or reverse these harmful effects in this experimental model.
  
  
  
  Systemic diseases such as hypercholesterolemia should be tightly controlled during the perioperative period of rotator cuff repair.
authors.affiliation: Department of Orthopaedic Surgery, Konkuk University School of Medicine, Seoul, Korea.
authors.forename: Seok Won
authors.initials: SW
authors.lastname: Chung
authors.affiliation: CM Chungmu Hospital, Seoul, Korea.
authors.forename: HaeBong
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Orthopaedic Surgery, National Police Hospital, Seoul, Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea Department of Pathology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam-si, Korea.
authors.forename: Ghee Young
authors.initials: GY
authors.lastname: Choe
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.
authors.forename: Sae Hoon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea ohjh1@snu.ac.kr.
authors.forename: Joo Han
authors.initials: JH
authors.lastname: Oh
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000502:physiology*; D004032:Diet; D004195:Disease Models, Animal; D005223:Fats / Q000378:metabolism*; D006937:Hypercholesterolemia / Q000503:physiopathology*; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D000070636:Rotator Cuff Injuries / Q000378:metabolism / Q000601:surgery*; D014945:Wound Healing
chemical_list: D005223:Fats
keywords: chronic rotator cuff tear; fatty infiltration; hypercholesterolemia; rabbit model; simvastatin; tendon-to-bone healing