title: Sevoflurane postconditioning affects post-ischaemic myocardial mitochondrial ATP-sensitive potassium channel function and apoptosis in ageing rats.
abstract: This study investigated the effect of sevoflurane postconditioning on post-ischaemic cardiac function, infarct size, myocardial mitochondrial ATP-sensitive potassium channel (mitoKATP) function and apoptosis in ageing rats to determine the possible mechanism underlying the cardioprotective property of sevoflurane. Ageing rat hearts were isolated and attached to a Langendorff apparatus. The hearts were then exposed or not to sevoflurane postconditioning in the presence or absence of 100 μmol/L 5-hydroxydecanoate (5-HD), a selective mitoKATP inhibitor. The infarct size was measured by triphenyltetrazolium chloride (TTC) staining. Mitochondrial morphology was observed by electron microscopy and scored using FlaMeng semiquantitative analysis. In addition, the expression levels of Bax, Bcl-2, and cytochrome-C (Cyt-C) were determined by Western blot analysis at the end of reperfusion. Sevoflurane postconditioning increased coronary flow, improved functional recovery, reduced Bax/Bcl-2 and Cyt-C phosphorylation levels, and decreased mitochondrial lesion severity and the extent of apoptosis. The protective effects of sevoflurane postconditioning were prevented by the mitoKATP inhibitor 5-HD. Sevoflurane postconditioning significantly protected the function of ageing hearts that were subjected to ischaemia and reperfusion, and these protective effects were mediated by mitoKATP opening.
authors.affiliation: Department of Anaesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's Hospital, Qingyuan, China.|Department of Anaesthesiology, Xuzhou Medical College, Xuzhou, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Jiang
authors.affiliation: Department of Anaesthesiology, Xuzhou Medical College, Xuzhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Anaesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's Hospital, Qingyuan, China.|Department of Anaesthesiology, Xuzhou Medical College, Xuzhou, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Anaesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's Hospital, Qingyuan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Anaesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's Hospital, Qingyuan, China.|Department of Anaesthesiology, Xuzhou Medical College, Xuzhou, China.
authors.forename: Zong-Hang
authors.initials: ZH
authors.lastname: Lin
authors.affiliation: Department of Anaesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's Hospital, Qingyuan, China.
authors.forename: Bao-Jun
authors.initials: BJ
authors.lastname: Fu
authors.affiliation: Department of Anaesthesiology, The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's Hospital, Qingyuan, China.
authors.forename: Yin-Ming
authors.initials: YM
authors.lastname: Zeng
mesh_terms: D000375:Aging; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D003326:Coronary Circulation / Q000187:drug effects; D003652:Decanoic Acids / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects; D006880:Hydroxy Acids / Q000494:pharmacology; D057775:Ischemic Postconditioning / Q000379:methods*; D054086:KATP Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017202:Myocardial Ischemia / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D003652:Decanoic Acids; D006880:Hydroxy Acids; D054086:KATP Channels; D008738:Methyl Ethers; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D000077149:Sevoflurane; C052853:5-hydroxydecanoic acid
keywords: ageing; apoptosis; ischaemic myocardia; mitochondrial ATP-sensitive potassium channels; sevoflurane postconditioning