title: Schisandrin B inhibits the proliferation of airway smooth muscle cells via microRNA-135a suppressing the expression of transient receptor potential channel 1.
abstract: Airway smooth muscle cell (ASMC) was known to involve in the pathophysiology of asthma. Schisandrin B was reported to have anti-asthmatic effects in a murine asthma model. However, the molecular mechanism involving in the effect of Schisandrin B on ASMCs remains poorly understood. Sprague-Dawley rats were divided into three groups: rats as the control (Group 1), sensitized rats (Group 2), sensitized rats and intragastric-administrated Schisandrin B (Group 3). The expression of miR-135a and TRPC1 was detected in the rats from three groups. Platelet-derived growth factor (PDGF)-BB was used to induce the proliferation of isolated ASMCs, and the expression of miR-135a and TRPC1 was detected in PDGF-BB-treated ASMCs. Cell viability was examined in ASMCs transfected with miR-135a inhibitor or si-TRPC1. The expression of TRPC1 was examined in A10 cells pretreated with miR-135a inhibitor or miR-135a mimic. In this study, we found that Schisandrin B attenuated the inspiratory and expiratory resistances in sensitized rats. Schisandrin B upregulated the mRNA level of miR-135a and decreased the expression of TRPC1 in sensitized rats. In addition, Schisandrin B reversed the expression of miR-135a and TRPC1 in PDGF-BB-induced ASMCs. Si-TRPC1 abrogated the increasing proliferation of ASMCs induced by miR-135a inhibitor. We also found that miR-135a regulated the expression of TRPC1 in the A10 cells. These results demonstrate that Schisandrin B inhibits the proliferation of ASMCs via miR-135a suppressing the expression of TRPC1.
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Luo-Xian
authors.initials: LX
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Xue-Yi
authors.initials: XY
authors.lastname: Tang
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Cui-Jie
authors.initials: CJ
authors.lastname: Tian
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: Cheng
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Xian-Liang
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Ma
authors.affiliation: Department of Respiratory Medicine, People's Hospital Affiliated to Zhengzhou University, 7 Weiwu Rd., Zhengzhou, 450003, China.
authors.forename: Zhuo-Chang
authors.initials: ZC
authors.lastname: Chen
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001249:Asthma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D034242:Cyclooctanes / Q000494:pharmacology; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011083:Polycyclic Compounds / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D050052:TRPC Cation Channels / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D034242:Cyclooctanes; D017705:Lignans; C000600409:MIRN135 microRNA, rat; D035683:MicroRNAs; D011083:Polycyclic Compounds; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; C015499:schizandrin B
keywords: airway smooth muscle cells; miR-135a; proliferation; schisandrin B; transient receptor potential 1