title: Coptis japonica Makino extract suppresses angiogenesis through regulation of cell cycle-related proteins.
abstract: Angiogenesis, neovascularization from pre-existing vessels, is a key step in tumor growth and metastasis, and anti-angiogenic agents that can interfere with these essential steps of cancer development are a promising strategy for human cancer treatment. In this study, we characterized the anti-angiogenic effects of Coptis japonica Makino extract (CJME) and its mechanism of action. CJME significantly inhibited the proliferation, migration, and invasion of vascular endothelial growth factor (VEGF)-stimulated HUVECs. Furthermore, CJME suppressed VEGF-induced tube formation in vitro and VEGF-induced microvessel sprouting ex vivo. According to our study, CJME blocked VEGF-induced cell cycle transition in G1. CJME decreased expression of cell cycle-regulated proteins, including Cyclin D, Cyclin E, Cdk2, and Cdk4 in response to VEGF. Taken together, the results of our study indicate that CJME suppresses VEGF-induced angiogenic events such as proliferation, migration, and tube formation via cell cycle arrest in G1.
authors.affiliation: a Yonsei-Fraunhofer Medical Device Lab, Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Seo Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: a Yonsei-Fraunhofer Medical Device Lab, Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Eok-Cheon
authors.initials: EC
authors.lastname: Kim
authors.affiliation: a Yonsei-Fraunhofer Medical Device Lab, Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Wan-Joong
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: a Yonsei-Fraunhofer Medical Device Lab, Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Myung-Hun
authors.initials: MH
authors.lastname: Lee
authors.affiliation: a Yonsei-Fraunhofer Medical Device Lab, Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: a Yonsei-Fraunhofer Medical Device Lab, Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Tack-Joong
authors.initials: TJ
authors.lastname: Kim
mesh_terms: D020533:Angiogenesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D039323:Coptis / Q000737:chemistry*; D056741:Cyclin D / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D019927:Cyclin E / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000494:pharmacology
chemical_list: D020533:Angiogenesis Inhibitors; D056741:Cyclin D; D019927:Cyclin E; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; C495894:CDK2 protein, human; C495900:CDK4 protein, human; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4
keywords: Coptis japonica Makino; angiogenesis; human umbilical vein endothelial cells (HUVEC); vascular endothelial growth factor (VEGF)