title: Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples.
abstract: A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Ren
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Mingjuan
authors.initials: M
authors.lastname: Yang
authors.affiliation: General Hospital of Heilongjiang Land Reclamation Bureau, Harbin, 150088, China.
authors.forename: Guoxia
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Wangjing Hospital, Academy of Traditional Chinese Medicine, 100102, Beijing, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Du
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Zhoujia
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China. Electronic address: zeliangchen@yahoo.com.
authors.forename: Zeliang
authors.initials: Z
authors.lastname: Chen
mesh_terms: D001769:Blood / Q000382:microbiology; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D045522:Recombinases / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D045522:Recombinases
keywords: Brucella; Point-of-care diagnosis; Recombinase polymerase amplification