title: Antiproliferative activity of Curcuma phaeocaulis Valeton extract using ultrasonic assistance and response surface methodology.
abstract: The objective of the study was to optimize the ultrasonic-assisted extraction of curdione, furanodienone, curcumol, and germacrone from Curcuma phaeocaulis Valeton (Val.) and investigate the antiproliferative activity of the extract. Under the suitable high-performance liquid chromatography condition, the calibration curves for these four tested compounds showed high levels of linearity and the recoveries of these four compounds were between 97.9 and 104.3%. Response surface methodology (RSM) combining central composite design and desirability function (DF) was used to define optimal extraction parameters. The results of RSM and DF revealed that the optimum conditions were obtained as 8 mL g-1for liquid-solid ratio, 70% ethanol concentration, and 20 min of ultrasonic time. It was found that the surface structures of the sonicated herbal materials were fluffy and irregular. The C. phaeocaulis Val. extract significantly inhibited the proliferation of RKO and HT-29 cells in vitro. The results reveal that the RSM can be effectively used for optimizing the ultrasonic-assisted extraction of bioactive components from C. phaeocaulis Val. for antiproliferative activity.
authors.affiliation: a Department of Clinical Pharmacology, Shanghai General Hospital , Shanghai Jiao Tong University School of Medicine , Shanghai , China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Department of Clinical Pharmacology, Shanghai General Hospital , Shanghai Jiao Tong University School of Medicine , Shanghai , China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: a Department of Clinical Pharmacology, Shanghai General Hospital , Shanghai Jiao Tong University School of Medicine , Shanghai , China.
authors.forename: Daode
authors.initials: D
authors.lastname: Hu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000494:pharmacology*; D014465:Ultrasonics
chemical_list: D010936:Plant Extracts
keywords: Antiproliferative activity; Curcuma phaeocaulis Valeton; response surface methodology; ultrasonic-assisted extraction