title: Effects of hyperbaric oxygen on the Nrf2 signaling pathway in secondary injury following traumatic brain injury.
abstract: We investigated the effects of hyperbaric oxygen treatment on the Nrf2 signaling pathway in secondary injury following traumatic brain injury, using a rat model. An improved Feeney freefall method was used to establish the rat traumatic brain injury model. Sixty rats were randomly divided into three groups: a sham surgery group, a traumatic brain injury group, and a group receiving hyperbaric oxygen treatment after traumatic brain injury. Neurological function scores were assessed at 12 and 24 h after injury. The expression levels of Nrf2, heme oxygenase 1 (HO-1), and quinine oxidoreductase 1 (NQO-1) in the cortex surrounding the brain lesion were detected by western blotting 24 h after the injury. Additionally, the TUNEL method was used to detect apoptosis of nerve cells 24 h after traumatic injury and Nissl staining was used to detect the number of whole neurons. Hyperbaric oxygen treatment significantly increased the expression of nuclear Nrf2 protein (P < 0.05), HO-1, and NQO-1 in the brain tissues surrounding the lesion after a traumatic brain injury (P < 0.05) and also significantly reduced the number of apoptotic and injured nerve cells. The neurological function scores also improved with hyperbaric oxygen treatment (P < 0.05). Therefore, hyperbaric oxygen has a neuroprotective role in traumatic brain injury, which is mediated by up-regulation of the Nrf2 signaling pathway.
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: X E
authors.initials: XE
authors.lastname: Meng
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: D F
authors.initials: DF
authors.lastname: Fan
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: D Z
authors.initials: DZ
authors.lastname: Guo
authors.affiliation: Department of Hyperbaric Oxygen, Navy General Hospital, Beijing, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Pan
mesh_terms: D000818:Animals; D008297:Male; D051381:Rats; D017209:Apoptosis; D000070642:Brain Injuries, Traumatic / Q000378:metabolism / Q000628:therapy; D002540:Cerebral Cortex / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics; D006931:Hyperbaric Oxygenation; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction; D015854:Up-Regulation; D051267:NF-E2-Related Factor 2 / Q000235:genetics
chemical_list: D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D016660:NAD(P)H Dehydrogenase (Quinone); C507703:NQO1 protein, rat; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat