title: Transcriptomic features associated with energy production in the muscles of Pacific bluefin tuna and Pacific cod.
abstract: Bluefin tuna are high-performance swimmers and top predators in the open ocean. Their swimming is grounded by unique features including an exceptional glycolytic potential in white muscle, which is supported by high enzymatic activities. Here we performed high-throughput RNA sequencing (RNA-Seq) in muscles of the Pacific bluefin tuna (Thunnus orientalis) and Pacific cod (Gadus macrocephalus) and conducted a comparative transcriptomic analysis of genes related to energy production. We found that the total expression of glycolytic genes was much higher in the white muscle of tuna than in the other muscles, and that the expression of only six genes for glycolytic enzymes accounted for 83.4% of the total. These expression patterns were in good agreement with the patterns of enzyme activity previously reported. The findings suggest that the mRNA expression of glycolytic genes may contribute directly to the enzymatic activities in the muscles of tuna.
authors.affiliation: a Graduate School of Bioresource and Bioenvironmental Sciences , Kyushu University , Fukuoka , Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Shibata
authors.affiliation: b Research Center for Aquatic Genomics, National Research Institute of Fisheries Science , Fisheries Research Agency , Yokohama , Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Mekuchi
authors.affiliation: c Biotechnology Research Institute for Drug Discovery , National Institute of Advanced Industrial Science and Technology (AIST) , Tokyo , Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Mori
authors.affiliation: d Laboratory of Molecular Gene Techniques, Division of Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture , Kyushu University , Fukuoka , Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Muta
authors.affiliation: e Division for Experimental Natural Science, Faculty of Arts and Science , Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka , Japan.
authors.forename: Vishwajit Sur
authors.initials: VS
authors.lastname: Chowdhury
authors.affiliation: b Research Center for Aquatic Genomics, National Research Institute of Fisheries Science , Fisheries Research Agency , Yokohama , Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: b Research Center for Aquatic Genomics, National Research Institute of Fisheries Science , Fisheries Research Agency , Yokohama , Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Ojima
authors.affiliation: b Research Center for Aquatic Genomics, National Research Institute of Fisheries Science , Fisheries Research Agency , Yokohama , Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Saitoh
authors.affiliation: f Fisheries Research Agency , Yokohama , Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: f Fisheries Research Agency , Yokohama , Japan.
authors.forename: Tokio
authors.initials: T
authors.lastname: Wada
authors.affiliation: g Japan Fisheries Science and Technology Association , Tokyo , Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Inouye
authors.affiliation: d Laboratory of Molecular Gene Techniques, Division of Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture , Kyushu University , Fukuoka , Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
authors.affiliation: d Laboratory of Molecular Gene Techniques, Division of Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture , Kyushu University , Fukuoka , Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D063990:Gene Ontology; D016678:Genome; D006019:Glycolysis / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D058977:Molecular Sequence Annotation; D009132:Muscles / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D013550:Swimming / Q000502:physiology; D059467:Transcriptome; D014413:Tuna / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger
keywords: Gadus macrocephalus; RNA-seq; Thunnus orientalis; glycolysis; warm-bloodedness