title: A genome-wide association study reveals novel elite allelic variations in seed oil content of Brassica napus.
abstract: A set of additive loci for seed oil content were identified using association mapping and one of the novel loci on the chromosome A5 was validated by linkage mapping. Increasing seed oil content is one of the most important goals in the breeding of oilseed crops including Brassica napus, yet the genetic basis for variations in this important trait remains unclear. By genome-wide association study of seed oil content using 521 B. napus accessions genotyped with the Brassica 60K SNP array, we identified 50 loci significantly associated with seed oil content using three statistical models, the general linear model, the mixed linear model and the Anderson-Darling test. Together, the identified loci could explain approximately 80 % of the total phenotypic variance, and 29 of these loci have not been reported previously. Furthermore, a novel locus on the chromosome A5 that could increase 1.5-1.7 % of seed oil content was validated in an independent bi-parental linkage population. Haplotype analysis showed that the favorable alleles for seed oil content exhibit cumulative effects. Our results thus provide valuable information for understanding the genetic control of seed oil content in B. napus and may facilitate marker-based breeding for a higher seed oil content in this important oil crop.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Chuchuan
authors.initials: C
authors.lastname: Fan
authors.affiliation: College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
authors.forename: Jiana
authors.initials: J
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Guangqin
authors.initials: G
authors.lastname: Cai
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Xinqi
authors.initials: X
authors.lastname: Yi
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China. ymzhou@mail.hzau.edu.cn.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D029688:Brassica napus / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D056726:Genetic Association Studies; D008040:Genetic Linkage; D005838:Genotype; D006239:Haplotypes; D015233:Models, Statistical; D000069600:Plant Breeding; D010938:Plant Oils / Q000032:analysis*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012639:Seeds / Q000737:chemistry*
chemical_list: D010938:Plant Oils