title: Oocyte aging in a marine protostome worm: The roles of maturation-promoting factor and extracellular signal regulated kinase form of mitogen-activated protein kinase.
abstract: The roles of maturation-promoting factor (MPF) and an extracellular signal regulated kinase form of mitogen-activated protein kinase (ERK MAPK) are analyzed during oocyte aging in the marine protostome worm Cerebratulus. About a day after removal from the ovary, unfertilized metaphase-I-arrested oocytes of Cerebratulus begin to flatten and swell before eventually lysing, thereby exhibiting characteristics of a necroptotic mode of regulated cell death. Based on immunoblots probed with phospho-specific antibodies, MPF and ERK are initially active in freshly mature specimens. However, as oocytes age, both kinase activities decline, with ERK deactivation occurring well before MPF downregulation. Experiments using pharmacological modulators indicate that oocyte degradation is promoted by the maturation-initiated activation of ERK as well as by the deactivation of MPF that occurs in extensively aged specimens. The potential significance of these findings is discussed relative to previously published results for apoptotic eggs and oocytes of echinoderm and vertebrate deuterostomes.
authors.affiliation: Department of Biology, MSC03 2020, 1 University of New Mexico, Albuquerque, NM, 87131, USA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Stricker
authors.affiliation: Department of Biology, MSC03 2020, 1 University of New Mexico, Albuquerque, NM, 87131, USA.
authors.forename: Bradley
authors.initials: B
authors.lastname: Beckstrom
authors.affiliation: Department of Biology, MSC03 2020, 1 University of New Mexico, Albuquerque, NM, 87131, USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Mendoza
authors.affiliation: Department of Biology, MSC03 2020, 1 University of New Mexico, Albuquerque, NM, 87131, USA.
authors.forename: Emma
authors.initials: E
authors.lastname: Stanislawski
authors.affiliation: Department of Biology, MSC03 2020, 1 University of New Mexico, Albuquerque, NM, 87131, USA.
authors.forename: Tewodros
authors.initials: T
authors.lastname: Wodajo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002070:Butadienes / Q000494:pharmacology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D007448:Invertebrates / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016200:Maturation-Promoting Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology; D000077546:Roscovitine; D012623:Seawater / Q000469:parasitology; D013997:Time Factors; D014665:Vasodilator Agents
chemical_list: D002070:Butadienes; D009570:Nitriles; D047428:Protein Kinase Inhibitors; D011687:Purines; C113580:U 0126; D014665:Vasodilator Agents; D000077546:Roscovitine; D005576:Colforsin; D016200:Maturation-Promoting Factor; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Cerebratulus; apoptosis; necroptosis; nemertean; non-apoptotic regulated cell death