title: Cell-Free Synthesis of a Functional Membrane Transporter into a Tethered Bilayer Lipid Membrane.
abstract: Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.
authors.affiliation: Flinders Centre for Nanoscale Science and Technology School of Chemical and Physical Sciences, Flinders University , Bedford Park, South Australia 5042, Australia.
authors.forename: Julius L
authors.initials: JL
authors.lastname: Zieleniecki
authors.affiliation: Australian Centre for Plant Functional Genomics School of Agriculture, Food, and Wine, University of Adelaide , Glen Osmond, South Australia 5064, Australia.
authors.forename: Yagnesh
authors.initials: Y
authors.lastname: Nagarajan
authors.affiliation: Australian Centre for Plant Functional Genomics School of Agriculture, Food, and Wine, University of Adelaide , Glen Osmond, South Australia 5064, Australia.
authors.forename: Shane
authors.initials: S
authors.lastname: Waters
authors.affiliation: Australian Centre for Plant Functional Genomics School of Agriculture, Food, and Wine, University of Adelaide , Glen Osmond, South Australia 5064, Australia.
authors.forename: Jay
authors.initials: J
authors.lastname: Rongala
authors.affiliation: Flinders Centre for Nanoscale Science and Technology School of Chemical and Physical Sciences, Flinders University , Bedford Park, South Australia 5042, Australia.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Thompson
authors.affiliation: Australian Centre for Plant Functional Genomics School of Agriculture, Food, and Wine, University of Adelaide , Glen Osmond, South Australia 5064, Australia.
authors.forename: Maria
authors.initials: M
authors.lastname: Hrmova
authors.affiliation: Flinders Centre for Nanoscale Science and Technology School of Chemical and Physical Sciences, Flinders University , Bedford Park, South Australia 5042, Australia.
authors.forename: Ingo
authors.initials: I
authors.lastname: Köper
mesh_terms: D001888:Boric Acids / Q000378:metabolism; D002474:Cell-Free System / Q000737:chemistry / Q000378:metabolism*; D058266:Dielectric Spectroscopy; D004952:Esters; D001467:Hordeum; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D008051:Lipid Bilayers / Q000737:chemistry*; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D008063:Thioctic Acid / Q000737:chemistry
chemical_list: D001888:Boric Acids; D004952:Esters; D008051:Lipid Bilayers; D026901:Membrane Transport Proteins; D010713:Phosphatidylcholines; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C021016:1,2-diphytanoylphosphatidylcholine; D008063:Thioctic Acid; C032688:boric acid