title: Mosquito saliva induced cutaneous events augment Chikungunya virus replication and disease progression.
abstract: Chikungunya virus (CHIKV) is transmitted when infected mosquito probes the host skin. While probing, mosquito saliva is expectorated into host skin along with virus which contains cocktail of molecules having anti-hemostatic and immunomodulatory properties. As mosquito saliva is a critical factor during natural arboviral infection, therefore we investigated mosquito saliva induced cutaneous events that modulate CHIKV infection. The effect of mosquito saliva on CHIKV infection was examined through inoculation of suckling mice subcutaneously with either CHIKV alone or uninfected mosquito bite followed by CHIKV. Histopathological evaluation of skin revealed infiltration of transmigrated inflammatory cells. Dermal blood vessels were hyperemic and adnexa showed degenerating lesions. Severe hemorrhage was observed in dermis and hypodermis in mosquito bite+CHIKV group compared to CHIKV group. Analysis of cytokines in skin showed significant downregulation of inflammatory genes like TLR-3, IL-2, IFN-γ, TNF-α and IFN-β in mosquito bite+CHIKV group compared to CHIKV group. In contrast, significant upregulation of anti-inflammatory genes like IL-4 and IL-10 was observed. These early events might have been responsible for increased dissemination of CHIKV to serum and peripheral organs as demonstrated through >10-fold higher viremia, antigen localization, cellular infiltration and degenerative changes. Thus mosquito saliva induced early cellular infiltration and associated cytokines augment CHIKV pathogenesis in a mouse model. This mosquito improved CHIKV mouse model simulates the realistic conditions that occur naturally during infected mosquito bite to a host. It will lead to better understanding of CHIKV pathobiology and promote the evaluation of novel medical countermeasures against emerging CHIKV.
authors.affiliation: Division of Virology, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: Division of Virology, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Joshi
authors.affiliation: Pharmacology and Toxicology Division, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India.
authors.forename: Durga P
authors.initials: DP
authors.lastname: Nagar
authors.affiliation: Vector Management Division, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India.
authors.forename: Ajay K
authors.initials: AK
authors.lastname: Sharma
authors.affiliation: Vector Management Division, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India.
authors.forename: D
authors.initials: D
authors.lastname: Sukumaran
authors.affiliation: Pharmacology and Toxicology Division, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India.
authors.forename: Satish C
authors.initials: SC
authors.lastname: Pant
authors.affiliation: Division of Virology, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India.
authors.forename: Man Mohan
authors.initials: MM
authors.lastname: Parida
authors.affiliation: Division of Virology, Defence R and D Establishment, Jhansi Road, Gwalior 474 002, M. P., India. Electronic address: pabandash@drde.drdo.in.
authors.forename: Paban Kumar
authors.initials: PK
authors.lastname: Dash
mesh_terms: D000818:Animals; D002460:Cell Line; D065632:Chikungunya Fever / Q000276:immunology / Q000635:transmission*; D002646:Chikungunya virus / Q000502:physiology*; D009033:Culicidae / Q000737:chemistry* / Q000821:virology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D051379:Mice; D012463:Saliva / Q000821:virology*; D014779:Virus Replication
chemical_list: D016207:Cytokines
keywords: Chikungunya; Cutaneous immune response; Mosquito saliva; Pathogenesis