title: [Construction of butanol-producing pathway from Clostridium saccharobutylicum in Escherichia coli JM109 (DE3) and its fermentation].
abstract: OBJECTIVE
  ] Several key genes (thlA, bcs-operon/crt-bcd1 -etfB2-fixB2-hbd and adhE) in butanol pathway from Clostridium saccharobutylicum DSM13864 were cloned, and a butanol-producing Escherichia coli strain was successfully constructed.
  
  
  METHODS
  Using genome of Clostridium saccharobutylicum DSM13864 as template, the key genes in butanol synthesis pathway were amplified, the recombinant plasmids pETDuet-bcs and pRSFDuet-thlA-adhE were constructed. Then the resultant plasmids were transformed into E. coli JM109 (DE3) to obtain E. coli BUT1 for butanol production, under the semi-anaerobic condition. Effects of different mediums on butanol production were studied.
  
  
  RESULTS
  The recombinant E. coli was capable of producing butanol (25.4 mg/L) under semi-anaerobic fermentation. After optimization on the fermentation medium, butanol titer reached 34.1 mg/L.
  
  
  CONCLUSIONS
  Butanol production by recombinant E. coli harboring exogenous butanol-producing pathway from Clostridium saccharobutylicum provides a feasible solution to overcome the hurdles in traditional butanol production approach by Clostridia.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Ruizhi
authors.initials: R
authors.lastname: Han
authors.forename: Guochao
authors.initials: G
authors.lastname: Xu
authors.forename: Jinjun
authors.initials: J
authors.lastname: Dong
authors.forename: Ye
authors.initials: Y
authors.lastname: Ni
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D060847:Metabolic Engineering
chemical_list: D001426:Bacterial Proteins; D020001:1-Butanol