title: Allergenicity attributes of different peanut market types.
abstract: Four different market classes of peanut (Runner, Virginia Spanish, and Valencia) are commonly consumed in Western countries, but for some consumers peanuts are a main cause of food-induced anaphylaxis. Limited information is available on the comparative allergenicity of these distinct market classes. The aim of this study was to compare allergenicity attributes of different peanut cultivars. The protein content and protein profiles were highly comparable for all tested cultivars. All cultivar samples contained the major allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6, as assessed by SDS-PAGE and RP-HPLC, although some minor differences in major allergen content were found between samples. All samples were reactive in commercial ELISAs for detection and quantification of peanut protein. IgE-binding potency differed between samples with a maximum factor of 2, indicating a highly comparable allergenicity. Based on our observations, we conclude that peanuts from the main market types consumed in Western countries are highly comparable in their allergenicity attributes, indicating that safety considerations with regard to peanut allergy are not dependent on the peanut cultivar in question.
authors.affiliation: Food Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska, 279 Food Innovation Center, Lincoln, NE 68588-6207, USA. Electronic address: stefkoppelman@zonnet.nl.
authors.forename: Stef J
authors.initials: SJ
authors.lastname: Koppelman
authors.affiliation: Food Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska, 279 Food Innovation Center, Lincoln, NE 68588-6207, USA.
authors.forename: Shyamali
authors.initials: S
authors.lastname: Jayasena
authors.affiliation: HAL Allergy, J.H. Oortweg 15-17, 2333 CH, Leiden, The Netherlands.
authors.forename: Dion
authors.initials: D
authors.lastname: Luykx
authors.affiliation: HAL Allergy, J.H. Oortweg 15-17, 2333 CH, Leiden, The Netherlands.
authors.forename: Erik
authors.initials: E
authors.lastname: Schepens
authors.affiliation: HAL Allergy, J.H. Oortweg 15-17, 2333 CH, Leiden, The Netherlands.
authors.forename: Danijela
authors.initials: D
authors.lastname: Apostolovic
authors.affiliation: TNO, Utrechtseweg 48, 3704 HE, Zeist, The Netherlands.
authors.forename: Govardus A H
authors.initials: GA
authors.lastname: de Jong
authors.affiliation: North Carolina State University, 210 Method Road, Greenhouse Unit 3, Raleigh, NC 27695-7629, USA.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Isleib
authors.affiliation: Food Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska, 279 Food Innovation Center, Lincoln, NE 68588-6207, USA.
authors.forename: Julie
authors.initials: J
authors.lastname: Nordlee
authors.affiliation: Food Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska, 279 Food Innovation Center, Lincoln, NE 68588-6207, USA.
authors.forename: Joe
authors.initials: J
authors.lastname: Baumert
authors.affiliation: Food Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska, 279 Food Innovation Center, Lincoln, NE 68588-6207, USA.
authors.forename: Steve L
authors.initials: SL
authors.lastname: Taylor
authors.affiliation: US Dept. of Agriculture, Agricultural Research Service, Food Allergy Research, 1100 Robert E. Lee blvd., New Orleans, LA 70124, USA.
authors.forename: Hsiaopo
authors.initials: H
authors.lastname: Cheng
authors.affiliation: US Dept. of Agriculture, Agricultural Research Service, Food Allergy Research, 1100 Robert E. Lee blvd., New Orleans, LA 70124, USA.
authors.forename: Soheila
authors.initials: S
authors.lastname: Maleki
mesh_terms: D000485:Allergens / Q000737:chemistry*; D010367:Arachis / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay
chemical_list: D000485:Allergens
keywords: Allergen; Arachis hypogaea; IgE; Immunoassay; Peanut