title: Enhanced (S)-linalool production by fusion expression of farnesyl diphosphate synthase and linalool synthase in Saccharomyces cerevisiae.
abstract: OBJECTIVE
  In order to improve the availability of geranyl diphosphate (GPP) in the mevalonate pathway for enhancing (S)-linalool production in Saccharomyces cerevisiae.
  
  
  RESULTS
  A (S)-linalool synthase (LIS): AaLS1 from Actinidia arguta was coexpressed with FPPS with different peptide linkers to redirect the flux from geranyl diphosphate (GPP) to (S)-linalool production in S. cerevisiae. The strain with the best peptide linker ((GGGGS)3 ), produced 101·55 ± 2·97 μg l(-1) (S)-linalool, a 69·7% increase compared to those with two independent LIS and FPPS expressed. In a 3-l fermenter, the (S)-linalool titre was further improved to 240·64 ± 5·31 μg l(-1) .
  
  
  CONCLUSIONS
  The results demonstrate that the fusion proteins catalysing consecutive steps in a metabolic pathway significantly improved the (S)-linalool production with GPP as precursor.
  
  
  CONCLUSIONS
  The fusion protein strategy co-expressing AaLS1 and FPPS, assembled with a long peptide linker made S. cerevisiae produced the highest reported (S)-Linalool titre to date.
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Mingxue
authors.initials: M
authors.lastname: Sun
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.|Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhou
mesh_terms: D029042:Actinidia / Q000201:enzymology*; D000080462:Acyclic Monoterpenes; D011756:Diphosphates / Q000378:metabolism; D004224:Diterpenes / Q000378:metabolism; D015870:Gene Expression; D051228:Geranyltranstransferase / Q000235:genetics / Q000378:metabolism*; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D039821:Monoterpenes / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000080462:Acyclic Monoterpenes; D011756:Diphosphates; D004224:Diterpenes; D039821:Monoterpenes; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C511282:geranyl diphosphate; C018584:linalool; D051228:Geranyltranstransferase; D006836:Hydro-Lyases; C092055:S-linalool synthase
keywords: biotechnology; enzymes; fermentation biotechnology; gene expression