title: Modulatory Role of Shorea robusta Bark on Glucose-metabolizing Enzymes in Diethylnitrosamine Induced Hepatocellular Carcinoma in Rats.
abstract: BACKGROUND
  The modulations of glucose-metabolizing enzyme activities play a vital rolein the depletion of energy metabolism and leads to inhibition of cancer growth.
  
  
  OBJECTIVE
  To find the effect of shorearobusta bark extract on glucose-metbolizing enzymes in diethylnitrosamine (DEN) induced hepatocellular carcinoma rats.
  
  
  METHODS
  Biochemical evaluation of glucose metabolizing enzyme were done in before and after shorearobusta bark extract (500mg/kg) treatment in DEN induced rats.
  
  
  RESULTS
  A significant increasein the activities of the key glycolytic enzymes viz., hexokinase and phosphoglucoisomerase, with a significant decrease in the gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatasewere observed in HCC bearing rats, when compared with the control. Administration of shorearobusta extract caused a significant decrease in theactivities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values.
  
  
  CONCLUSIONS
  The current findings suggest that the S. robusta extract has a definite modulating role on the key enzymes ofglucose-metabolism in HCC. The modulatory effect may be due to the phytoactive constituents present in the extract of S. robusta.
  
  
  CONCLUSIONS
  Administration of shorea robusta bark extract caused a significant decrease in the activities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values. The S. robusta extract has modulatory activity on the carbohydrate metabolism in DEN-induced HCC bearing rats through a mechanism that which does not provoke any acute biochemical disturbances in the metabolic pathways of glycolysis and gluconeogenesis. The modulatory effect of S. robusta extract may be attributed to the presence of active compounds such as polyphenols and flavonoids. Abbreviations used: HCC: Hepatocellular Carcinoma, SRBE: Shorearobusta bark extract; HEX: Hexokinase; PGI: Phosphoglucoisomerase; DEN: Diethylnitrosamine.
authors.affiliation: Centre for Research and Development, Marudupandiyar College, Thanjavur, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Kalaiselvan
authors.affiliation: Centre for Research and Development, Marudupandiyar College, Thanjavur, Tamil Nadu, India.
authors.forename: T
authors.initials: T
authors.lastname: Anand
authors.affiliation: Department of Chemistry, PRIST University, Thanjavur, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Gokulakrishnan
authors.affiliation: Centre for Research and Development, Marudupandiyar College, Thanjavur, Tamil Nadu, India.
authors.forename: M C
authors.initials: MC
authors.lastname: Kamaraj
authors.affiliation: Department of Biochemistry, Marudupandiyar College, Thanjavur, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Velavan
keywords: Diethylnitrosamine; Shorea robusta; glucose-metabolizing enzymes; hepatocellular carcinoma