title: Production of Escherichia coli-based virus-like particle vaccine against porcine circovirus type 2 challenge in piglets: Structure characterization and protective efficacy validation.
abstract: We report the strategies leading to the large-production of soluble non-tag full-length porcine circovirus type 2 (PCV2) Cap protein in Escherichia coli. Under neutral pH condition, the purified recombinant Cap protein derived from E. coli expression self-assembles into homogenous round virus-like particle at the similar size of that of the intact PCV2 virus, which is further characterized by Cryo-EM single particle structure determined at 4.5Å. The engineered PCV2 rCap VLP was tested as a subunit vaccine for the protective efficacy against PCV2 challenge on 3-week old piglets. Similar to commercial available PCV2 vaccine, the Cap VLP-immunized piglets developed specific antibody-mediated response and were protected from the virulent SH PCV2 strain challenge. Hence, the production of E. coli based PCV2Cap-VLP could be applied as a cost-friendly and effective subunit vaccine to control PCV2 spreading in developing countries.
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Xi
authors.affiliation: National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, Suzhou Industrial Park, Jiangsu 215123, PR China; Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore, Singapore.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Mo
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, Suzhou Industrial Park, Jiangsu 215123, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yin
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Chaochao
authors.initials: C
authors.lastname: Lv
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Sun
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Yang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Yao
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Xuan
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Li
authors.affiliation: National University of Singapore (Suzhou) Research Institute, 377 Lin Quan Street, Suzhou Industrial Park, Jiangsu 215123, PR China; Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore, Singapore. Electronic address: dbsyya@nusri.cn.
authors.forename: Y Adam
authors.initials: YA
authors.lastname: Yuan
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, PR China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, PR China. Electronic address: tiankg@263.net.
authors.forename: Kegong
authors.initials: K
authors.lastname: Tian
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D018173:Circoviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary*; D017925:Circovirus / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D013552:Swine; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D019065:Virus Assembly
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D022223:Vaccines, Subunit; D058425:Vaccines, Virus-Like Particle
keywords: Cryo-EM structure; Escherichia coli expression; Porcine circovirus type 2; Vaccine efficacy; Virus-like particle