title: Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu.
abstract: The present study emphasizes the diversity assessment of marine Bacillus species with special reference to biosurfactant production, respective gene expression, and discrimination among Bacillus licheniformis and Bacillus subtilis. Among the 200 individual species of eastern coastal plain of Tamil Nadu screened, five biosurfactant producing potential bacterial species with entirely different morphology were selected. Biochemical and 16S rRNA gene sequence analysis suggested that all the said five species belong to Bacillus genera but differ in species levels. Biosurfactant of all the five species fluctuates in greater levels with respect to activity as well as to constituents but showed partial similarity to the commercially available surfactin. The expression of srf gene was realized in all of the five species. However, the sfp gene expression was observed only in three species. In conclusion, both B. licheniformis and B. subtilis demonstrate srf gene; nevertheless, sfp gene was expressed only by Bacillus subtilis.
authors.affiliation: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India.
authors.forename: Sreethar
authors.initials: S
authors.lastname: Swaathy
authors.affiliation: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India.
authors.forename: Varadharajan
authors.initials: V
authors.lastname: Kavitha
authors.affiliation: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India.
authors.forename: Arockiasamy
authors.initials: A
authors.lastname: Sahaya Pravin
authors.affiliation: Environmental Technology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Sekaran
authors.affiliation: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India.
authors.forename: Asit Baran
authors.identifier: 0000-0001-7953-941X
authors.initials: AB
authors.lastname: Mandal
authors.affiliation: Microbiology Division, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamil Nadu 600 020, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Gnanamani