title: Value of Functionalized Superparamagnetic Iron Oxide Nanoparticles in the Diagnosis and Treatment of Acute Temporal Lobe Epilepsy on MRI.
abstract: Purpose. Although active targeting of drugs using a magnetic-targeted drug delivery system (MTDS) with superparamagnetic iron oxide nanoparticles (SPIONs) is a very effective treatment approach for tumors and other illnesses, successful results of drug-resistant temporal lobe epilepsy (TLE) are unprecedented. A hallmark in the neuropathology of TLE is brain inflammation, in particular the activation of interleukin-1β (IL-1β) induced by activated glial cells, which has been considered a new mechanistic target for treatment. The purpose of this study was to determine the feasibility of the functionalized SPIONs with anti-IL-1β monoclonal antibody (mAb) attached to render MRI diagnoses and simultaneously provide targeted therapy with the neutralization of IL-1β overexpressed in epileptogenic zone of an acute rat model of TLE. Experimental Design. The anti-IL-1β mAb-SPIONs were studied in vivo versus plain SPIONs and saline. Lithium-chloride pilocarpine-induced TLE models (n = 60) were followed by Western blot, Perl's iron staining, Nissl staining, and immunofluorescent double-label staining after MRI examination. Results. The magnetic anti-IL-1β mAb-SPION administered intravenously, which crossed the BBB and was concentrated in the astrocytes and neurons in epileptogenic tissues, rendered these tissues visible on MRI and simultaneously delivered anti-IL-1β mAb to the epileptogenic focus. Conclusions. Our study provides the first evidence that the novel approach enhanced accumulation and the therapeutic effect of anti-IL-1β mAb by MTDS using SPIONs.
authors.affiliation: Department of Neurology, Affiliated Hospital of Jining Medical University, Guhuai Road, No. 79, Jining, Shandong 272000, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Fu
authors.affiliation: Department of Neurology, Affiliated Hospital of Jining Medical University, Guhuai Road, No. 79, Jining, Shandong 272000, China.
authors.forename: Qingxia
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Department of Magnetic Resonance Imaging, Affiliated Hospital of Jining Medical University, Guhuai Road, No. 79, Jining, Shandong 272000, China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Department of Magnetic Resonance Imaging, Affiliated Hospital of Jining Medical University, Guhuai Road, No. 79, Jining, Shandong 272000, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Gao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001253:Astrocytes / Q000378:metabolism; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000276:immunology; D006624:Hippocampus / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors* / Q000276:immunology; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D009474:Neurons / Q000378:metabolism; D010862:Pilocarpine; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000911:Antibodies, Monoclonal; D053583:Interleukin-1beta; D058185:Magnetite Nanoparticles; D010862:Pilocarpine