title: Laccase-Functionalized Graphene Oxide Assemblies as Efficient Nanobiocatalysts for Oxidation Reactions.
abstract: Multi-layer graphene oxide-enzyme nanoassemblies were prepared through the multi-point covalent immobilization of laccase from Trametes versicolor (TvL) on functionalized graphene oxide (fGO). The catalytic properties of the fGO-TvL nanoassemblies were found to depend on the number of the graphene oxide-enzyme layers present in the nanostructure. The fGO-TvL nanoassemblies exhibit an enhanced thermal stability at 60 °C, as demonstrated by a 4.7-fold higher activity as compared to the free enzyme. The multi-layer graphene oxide-enzyme nanoassemblies can efficiently catalyze the oxidation of anthracene, as well as the decolorization of an industrial dye, pinacyanol chloride. These materials retained almost completely their decolorization activity after five reaction cycles, proving their potential as efficient nano- biocatalysts for various applications.
authors.affiliation: Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina 45110, Greece. michaelapatila@gmail.com.
authors.forename: Michaela
authors.initials: M
authors.lastname: Patila
authors.affiliation: Department of Materials Science and Engineering, University of Ioannina, Ioannina 45110, Greece. antoniokoul@gmail.com.|Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. antoniokoul@gmail.com.
authors.forename: Antonios
authors.initials: A
authors.lastname: Kouloumpis
authors.affiliation: Department of Materials Science and Engineering, University of Ioannina, Ioannina 45110, Greece. dgourni@uoi.gr.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Gournis
authors.affiliation: Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. p.rudolf@rug.nl.
authors.forename: Petra
authors.initials: P
authors.lastname: Rudolf
authors.affiliation: Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina 45110, Greece. hstamati@uoi.gr.
authors.forename: Haralambos
authors.initials: H
authors.lastname: Stamatis
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004798:Enzymes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D042845:Laccase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry
chemical_list: D004798:Enzymes; D010087:Oxides; D006108:Graphite; D042845:Laccase
keywords: graphene oxide; immobilization; laccase; nanoassemblies; nanobiocatalysis