title: Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon).
abstract: MAT1 (ménage à trois 1), an assembly factor and targeting subunit of the CDK-dependent kinase (CAK), can regulate the cell cycle, transcription, and DNA repair. This study was intended to investigate the role of MAT1 in the reproductive maturation of black tiger shrimp (Penaeus monodon). In this study, the P. monodon MAT1 (PmMAT1) gene was identified and characterized. The full-length cDNA of PmMAT1 was 1490 bp in length with an open-reading frame of 993 bp corresponding to 330 amino acids. The temporal expression of PmMAT1 in various tissues was measured by quantitative real-time PCR with the highest expression observed in ovaries. In the ovaries, the PmMAT1 gene was continuously but differentially expressed during the maturation stages. Comparative analyses of MAT1, CDK7, and cyclin H in the CAK complex of P. monodon indicated that the expression of CDK7 and cyclin H coincided with that of MAT1 during the ovary maturation stages. Serotonin (5-HT) injection promoted the expression level of PmMAT1 in the ovaries of shrimp at 6-48 h post-injection. These results indicate that PmMat1 plays a prominent role in the process of ovarian maturation.
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|College of Aqua-Life Science and Technology, Shanghai Ocean University, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, College of Environmental Science and Engineering, Yangzhou University, Guangzhou, China.
authors.forename: M J
authors.initials: MJ
authors.lastname: Fu
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, College of Environmental Science and Engineering, Yangzhou University, Guangzhou, China.
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, College of Environmental Science and Engineering, Yangzhou University, Guangzhou, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Bao
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, College of Environmental Science and Engineering, Yangzhou University, Guangzhou, China.
authors.forename: F L
authors.initials: FL
authors.lastname: Zhou
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, College of Environmental Science and Engineering, Yangzhou University, Guangzhou, China.|Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Sanya, China.
authors.forename: Q B
authors.initials: QB
authors.lastname: Yang
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, College of Environmental Science and Engineering, Yangzhou University, Guangzhou, China.
authors.forename: S G
authors.initials: SG
authors.lastname: Jiang
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.|Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, College of Environmental Science and Engineering, Yangzhou University, Guangzhou, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment; D012741:Sexual Maturation
chemical_list: D060829:Arthropod Proteins