title: Reinforcement of transvaginal repair using polypropylene mesh functionalized with basic fibroblast growth factor.
abstract: Numerous modifications have been developed over the past two decades seeking to improve the transvaginal repair in the pelvic organ prolapse (POP) by using polypropylene (PP) mesh implants. The hydrophobicity of PP, however, presents a great hindrance for translating potential technologies into viable clinical applications. In this study, by manipulating self-polymerization and strong adhesive characteristics of dopamine, we developed a facile method to enhance the transvaginal repair by modifying PP meshes with polydopamine (PDA), which allowed easy grafting of basic fibroblast growth factor (bFGF) onto the surface of PP. Such surface modification of PP meshes with bFGF was found to efficiently promote bioactivity without changing the morphology or mechanical properties of the PP meshes. Additionally, bFGF-modified PP meshes significantly promoted cell viability and adhesion compared to the unmodified PP. Ultimately, after three months of implantation, the bFGF-modified PP meshes exhibited improved tissue repair with greater degree of organization of deposited collagen, increased tensile strength and reduced inflammatory response. Overall, the surface-modified PP meshes will be highly practical as templates for transvaginal repair in the POP treatment.
authors.affiliation: Department of Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, 910 Hengshan Road, Shanghai 230030, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: John A. Paulson School of Engineering and Applied Sciences，Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA.
authors.forename: Zhi Yuan William
authors.initials: ZYW
authors.lastname: Lin
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China.
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Cheng
authors.affiliation: Department of general surgery, Yangzhou First Hospital Affiliated to Southeast University, 365 Hanjiang Middle Road, Yangzhou, Jiangsu 225000, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.affiliation: John A. Paulson School of Engineering and Applied Sciences，Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Gynecology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, 910 Hengshan Road, Shanghai 230030, PR China. Electronic address: superstarcxl@126.com.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, PR China. Electronic address: wgcui80@hotmail.com.
authors.forename: Wenguo
authors.initials: W
authors.lastname: Cui
mesh_terms: D034861:Abdominal Wall / Q000601:surgery*; D058107:Abdominal Wound Closure Techniques; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D008954:Models, Biological; D056887:Pelvic Organ Prolapse / Q000601:surgery; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011126:Polypropylenes / Q000737:chemistry / Q000494:pharmacology*; D061251:Primary Cell Culture; D019736:Prostheses and Implants; D011817:Rabbits; D013526:Surgical Mesh; D013718:Tensile Strength / Q000187:drug effects; D014621:Vagina
chemical_list: D015415:Biomarkers; D007211:Indoles; D011108:Polymers; D011126:Polypropylenes; C568283:polydopamine; D016222:Fibroblast Growth Factor 2; D003094:Collagen
keywords: Basic fibroblast growth factor; Pelvic organ prolapsed; Polydopamine; Polypropylene mesh; Transvaginal repair