title: Simultaneous estimation of levodopa, carbidopa and 3-oxymethyldopa in rat plasma using HPLC-ECD.
abstract: The aim of study was to develop a suitable analytical method for simultaneous estimation of levodopa, carbidopa and 3-O-methyl dopa in rat plasma. Chromatographic separation of plasma samples was achieved using a reverse-phase C18 column. The mobile phase used consisted of a mixture of methanol and phosphate buffer (10 mM, pH 3.50) in the ratio of 90:10 v/v. All analytes were estimated by electrochemical detection at +800 mV. The developed method has been validated as per the standard guidelines. Precision study results were found to be satisfactory, with percentage relative standard deviation for repeatability and intermediate precision <3.96 and 6.56%, respectively, for all analytes detected in rat plasma. The developed method in rat plasma was found to be simple, rapid, accurate, precise and specific. The proposed method has been successfully applied for analysis of rat plasma samples obtained during an oral pharmacokinetic study of sustained release pellets of levodopa and carbidopa in rats. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Pilani Campus, Rajasthan, - 333 031, India.
authors.forename: Prashant P
authors.initials: PP
authors.lastname: Raut
authors.affiliation: Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, Telangana, - 500 078, India.
authors.forename: Shrikant Y
authors.initials: SY
authors.lastname: Charde
authors.affiliation: Industrial Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Pilani Campus, Rajasthan, - 333 031, India.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Bishnoi
mesh_terms: D000818:Animals; D002230:Carbidopa / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000097:blood; D055664:Electrochemical Techniques / Q000379:methods*; D007980:Levodopa / Q000097:blood*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014443:Tyrosine / Q000031:analogs & derivatives
chemical_list: D014443:Tyrosine; D007980:Levodopa; D004295:Dihydroxyphenylalanine; D002230:Carbidopa; C008404:3-methoxytyrosine
keywords: 3-O-methyl dopa; HPLC-ECD; bioanalytical method; carbidopa; levodopa