title: In vivo photodynamic inactivation of Candida albicans using chloro-aluminum phthalocyanine.
abstract: This study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro-aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions (NE) and chloro-aluminum phthalocyanine (ClAlP) diluted in DMSO (DMSO) as photosensitizer (PS). Seventy-five 6-week-old female Swiss mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDT was performed on the tongue by the application of the photosensitizers and LED light (100 J cm(-2) -660 nm). Twenty-four hours and 7 days after treatments, microbiological evaluation was carried out by recovering C. albicans from the tongue of animals (CFU ml(-1) ). Then, mice were sacrificed and the tongues were surgically removed for histological and biomolecular analysis of pro- and anti-inflammatory cytokines. Data were analyzed by ANOVA followed by Tukey's post hoc test. ClAlP-NE-mediated PDT reduced 2.26 log10 of C. albicans recovered from the tongue when compared with the control group (P-L-) (P < 0.05). PDT did not promote adverse effects on the tongue tissue. Seven days after treatment, all animals were completely healthy. In summary, PDT mediated by chloro-aluminum phthalocyanine entrapped in cationic nanoemulsions was effective in reducing C. albicans recovered from the oral lesions of immunocompromised mice.
authors.affiliation: Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP- Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Carmello
authors.affiliation: Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP- Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Alves
authors.affiliation: Department of Dentistry, School of Health Science, UnB - University of Brasília, Brasília, Brazil.
authors.forename: Apd
authors.initials: A
authors.lastname: Ribeiro
authors.affiliation: Department of Physiology and Pathology, Araraquara Dental School, UNESP- Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: F G
authors.initials: FG
authors.lastname: Basso
authors.affiliation: Department of Physiology and Pathology, Araraquara Dental School, UNESP- Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: de Souza Costa
authors.affiliation: Center of Nanotechnology and Tissue Engineers, Photobiology and Photomedicine Research Group, FFCLRP-São Paulo University, Ribeirão Preto, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Tedesco
authors.affiliation: Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, UNESP- Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: F L
authors.initials: FL
authors.lastname: Primo
authors.affiliation: Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP- Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: E G
authors.initials: EG
authors.lastname: Mima
authors.affiliation: Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP- Univ Estadual Paulista, Araraquara, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Pavarina
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000187:drug effects* / Q000302:isolation & purification / Q000528:radiation effects*; D002180:Candidiasis, Oral / Q000188:drug therapy* / Q000382:microbiology; D016207:Cytokines / Q000032:analysis / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D007211:Indoles / Q000494:pharmacology*; D051379:Mice; D009942:Organometallic Compounds / Q000494:pharmacology*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology; D011897:Random Allocation; D013752:Tetracycline / Q000494:pharmacology; D014059:Tongue / Q000187:drug effects / Q000382:microbiology / Q000528:radiation effects
chemical_list: D016207:Cytokines; D007211:Indoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; C044797:chloroaluminum phthalocyanine; D013752:Tetracycline
keywords: Candida albicans; chloro-aluminum phthalocyanine; murine model