title: Multidimensional Separation of Natural Products Using Liquid Chromatography Coupled to Hadamard Transform Ion Mobility Mass Spectrometry.
abstract: A high performance liquid chromatograph (HPLC)was interfaced to an atmospheric drift tube ion mobility time of flight mass spectrometry. The power of multidimensional separation was demonstrated using chili pepper extracts. The ambient pressure drift tube ion mobility provided high resolving powers up to 166 for the HPLC eluent. With implementation of Hadamard transform (HT), the duty cycle for the ion mobility drift tube was increased from less than 1% to 50%, and the ion transmission efficiency was improved by over 200 times compared with pulsed mode, improving signal to noise ratio 10 times. HT ion mobility and TOF mass spectrometry provide an additional dimension of separation for complex samples without increasing the analysis time compared with conventional HPLC. Graphical Abstract ᅟ.
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, Xinjiang, 843300, China.|Department of Chemistry, Washington State University, Pullman, WA, 99164-4630, USA.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, 99164-4630, USA.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tofwerk, AG, Uttigenstrasse 22, CH-3600, Thun, Switzerland.
authors.forename: Richard
authors.initials: R
authors.lastname: Knochenmuss
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, 99164-4630, USA.
authors.forename: William F
authors.initials: WF
authors.lastname: Siems
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA, 99164-4630, USA. hhhill@wsu.edu.
authors.forename: Herbert H
authors.initials: HH
authors.lastname: Hill
keywords: Chili pepper; ESI; HPLC; Hadamard transform; IMS; TOF-MS