title: Crystal structure of maize serine racemase with pyridoxal 5'-phosphate.
abstract: Serine racemase (SR) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that is responsible for D-serine biosynthesis in vivo. The first X-ray crystal structure of maize SR was determined to 2.1 Å resolution and PLP binding was confirmed in solution by UV-Vis absorption spectrometry. Maize SR belongs to the type II PLP-dependent enzymes and differs from the SR of a vancomycin-resistant bacterium. The PLP is bound to each monomer by forming a Schiff base with Lys67. Structural comparison with rat and fission yeast SRs reveals a similar arrangement of active-site residues but a different orientation of the C-terminal helix.
authors.affiliation: School of Life Science, Anhui Agricultural University, Hefei, Anhui 230036, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zou
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Life Science, Anhui Agricultural University, Hefei, Anhui 230036, People's Republic of China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Sun
authors.affiliation: School of Life Science, Anhui Agricultural University, Hefei, Anhui 230036, People's Republic of China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Life Science, Anhui Agricultural University, Hefei, Anhui 230036, People's Republic of China.
authors.forename: Beijiu
authors.initials: B
authors.lastname: Cheng
authors.affiliation: School of Life Science, Anhui Agricultural University, Hefei, Anhui 230036, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D008958:Models, Molecular; D010940:Plant Proteins / Q000737:chemistry*; D000072756:Protein Conformation, alpha-Helical; D020836:Protein Structure, Quaternary; D011732:Pyridoxal Phosphate / Q000737:chemistry; D019998:Racemases and Epimerases; D040681:Structural Homology, Protein; D003313:Zea mays / Q000201:enzymology*; D000099300:Serine Racemase
chemical_list: D010940:Plant Proteins; D011732:Pyridoxal Phosphate; D019998:Racemases and Epimerases; D000099300:Serine Racemase
keywords: cofactor; crystal structure; maize; pyridoxal 5′-phosphate; serine racemase; structural comparison