title: Sequential molecularly imprinted solid-phase extraction methods for the analysis of resveratrol and other polyphenols.
abstract: Molecularly imprinted polymers (MIPs) templated with either the phytoalexin, (E)-resveratrol, or its structural analog, 3,5-dihydroxy-N-(4-hydroxyphenyl)benzamide, have been used in tandem for the sequential extraction of (E)-resveratrol from aqueous peanut meal extracts in high purity and in near quantitative yields. Re-processing of the (E)-resveratrol-depleted peanut meal extract with the 3,5-dihydroxy-N-(4-hydroxyphenyl)benzamide imprinted MIP yielded additional polyphenolic components, identified as A-type procyanidins. Tandem liquid chromatography-electrospray ionization mass spectrometry confirmed the identity and purity of the isolated products. This study documents the advantages of tandem approaches with MIPs for the solid phase extraction and analysis of multiple bioactive compounds present in complex biomass waste streams.
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Lachlan J
authors.initials: LJ
authors.lastname: Schwarz
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Basil
authors.initials: B
authors.lastname: Danylec
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Harris
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.
authors.forename: Reinhard I
authors.initials: RI
authors.lastname: Boysen
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia. Electronic address: milton.hearn@monash.edu.
authors.forename: Milton T W
authors.initials: MT
authors.lastname: Hearn
mesh_terms: D010367:Arachis / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D007220:Industrial Waste / Q000032:analysis; D054802:Molecular Imprinting; D059808:Polyphenols / Q000032:analysis* / Q000302:isolation & purification*; D000077185:Resveratrol; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000032:analysis* / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry
chemical_list: D007220:Industrial Waste; D059808:Polyphenols; D013267:Stilbenes; D014867:Water; D000077185:Resveratrol
keywords: Molecularly imprinted polymers; Molecularly imprinted solid-phase extraction; Peanut meal; Polyphenolics; Procyanidins; Resveratrol