title: Lack of association between polymorphic copies of endogenous Jaagsiekte sheep retrovirus (enJSRVs) and Ovine Pulmonary Adenocarcinoma.
abstract: Ovine Pulmonary Adenocarcinoma (OPA) is a retrovirus-induced lung tumor of sheep, goat and mouflon, and its etiologic agent, Jaagsiekte sheep retrovirus (JSRV) is the only virus known to cause a naturally occurred lung adenocarcinoma. The oncogenic JSRV has several endogenous counterparts termed enJSRVs, some of which have been shown to interfere with JSRV replication at early and late stages of the retroviral cycle inhibiting JSRV exit from the cell, and thus, protecting sheep against the infection. In this work, Latxa sheep breed animals were classified depending on the presence/absence of OPA-characteristic clinical lesions in the lung. Using a PCR genotyping method and a logistic regression-based association study, five polymorphic enJSRV copies were analyzed in 49 OPA positive sheep and 124 control individuals. Our results showed that the frequency of the provirus enJSRV-16 is much higher in Latxa sheep breed than in other breeds, suggesting a recent proliferation of this provirus in the studied breed. However, no polymorphic enJSRV was found to be statistically associated with the susceptibility/resistance to OPA development.
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Maialen
authors.initials: M
authors.lastname: Sistiaga-Poveda
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Amaia
authors.initials: A
authors.lastname: Larruskain
authors.affiliation: Applied Mathematics, Statistics and Operative Research Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain.
authors.forename: Maider
authors.initials: M
authors.lastname: Mateo-Abad
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain. Electronic address: begonamarina.jugo@ehu.eus.
authors.forename: Begoña M
authors.initials: BM
authors.lastname: Jugo
mesh_terms: D000230:Adenocarcinoma / Q000662:veterinary* / Q000821:virology; D000077192:Adenocarcinoma of Lung; D000818:Animals; D001947:Breeding; D022141:Jaagsiekte sheep retrovirus / Q000235:genetics*; D008175:Lung Neoplasms / Q000662:veterinary* / Q000821:virology; D011110:Polymorphism, Genetic; D011533:Proviruses / Q000502:physiology*; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*; D013045:Species Specificity
keywords: Disease association; ERVs; JSRV; OPA; Respiratory diseases