title: Superparamagnetic Fe3O4 Nanoparticles: Synthesis by Thermal Decomposition of Iron(III) Glucuronate and Application in Magnetic Resonance Imaging.
abstract: Monodisperse superparamagnetic Fe3O4 nanoparticles coated with oleic acid were prepared by thermal decomposition of Fe(III) glucuronate. The shape, size, and particle size distribution were controlled by varying the reaction parameters, such as the reaction temperature, concentration of the stabilizer, and type of high-boiling-point solvents. Magnetite particles were characterized by transmission electron microscopy (TEM), as well as electron diffraction (SAED), X-ray diffraction (XRD), dynamic light scattering (DLS), and magnetometer measurements. The particle coating was analyzed by atomic absorption spectroscopy (AAS) and attenuated total reflection (ATR) Fourier transform infrared spectroscopy (FTIR) spectroscopy. To make the Fe3O4 nanoparticles dispersible in water, the particle surface was modified with α-carboxyl-ω-bis(ethane-2,1-diyl)phosphonic acid-terminated poly(3-O-methacryloyl-α-D-glucopyranose) (PMG-P). For future practical biomedical applications, nontoxicity plays a key role, and the PMG-P&Fe3O4 nanoparticles were tested on rat mesenchymal stem cells to determine the particle toxicity and their ability to label the cells. MR relaxometry confirmed that the PMG-P&Fe3O4 nanoparticles had high relaxivity but rather low cellular uptake. Nevertheless, the labeled cells still provided visible contrast enhancement in the magnetic resonance image. In addition, the cell viability was not compromised by the nanoparticles. Therefore, the PMG-P&Fe3O4 nanoparticles have the potential to be used in biomedical applications, especially as contrast agents for magnetic resonance imaging.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Vitalii
authors.initials: V
authors.lastname: Patsula
authors.affiliation: Institute for Clinical and Experimental Medicine , Vídeňská 1958/9, 140 21 Prague 4, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Kosinová
authors.affiliation: University of Zagreb School of Medicine , Croatian Institute for Brain Research, Salata 12, HR-10000 Zagreb, Croatia.
authors.forename: Marija
authors.initials: M
authors.lastname: Lovrić
authors.affiliation: University of Zagreb School of Medicine , Croatian Institute for Brain Research, Salata 12, HR-10000 Zagreb, Croatia.
authors.forename: Lejla
authors.initials: L
authors.lastname: Ferhatovic Hamzić
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Mariia
authors.initials: M
authors.lastname: Rabyk
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Rafal
authors.initials: R
authors.lastname: Konefal
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Paruzel
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Šlouf
authors.affiliation: Institute for Clinical and Experimental Medicine , Vídeňská 1958/9, 140 21 Prague 4, Czech Republic.
authors.forename: Vít
authors.initials: V
authors.lastname: Herynek
authors.affiliation: University of Zagreb School of Medicine , Croatian Institute for Brain Research, Salata 12, HR-10000 Zagreb, Croatia.
authors.forename: Srećko
authors.initials: S
authors.lastname: Gajović
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic , Heyrovského nám. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051381:Rats; D011917:Rats, Inbred Lew; D055647:Rats, Transgenic
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D020723:Glucuronic Acid
keywords: iron oxide; magnetic resonance imaging; nanoparticles; superparamagnetic; thermal decomposition