title: Immunoexpression of HPV 16/18 E6 and E7 oncoproteins in high-grade cervical squamous intraepithelial lesions in HIV-positive women.
abstract: The aim of this study was to assess the immunoexpression of human papillomavirus genotypes 16 and 18 (E6 and E7) oncoproteins in cervical high-grade squamous intraepithelial lesions (HSIL) of human immunodeficiency virus (HIV)-positive women. These results were also compared to the persistence and/or recurrence of lesions after loop electrosurgical excision procedure. Cervical samples from 158 patients were divided into three groups according to the presence or absence of HSIL in women who were or were not HIV-positive. By using the tissue microarray technique, immunohistochemistry was performed to analyze the expression of HPV 16/18 E6 and E7 oncoproteins. Cervical samples from 95 HIV-positive women and 63 HIV-negative women were studied. A statistically significant difference was found in the immunoexpression of E6 and E7 oncoproteins in samples from HIV-positive women with HSIL and that of women with non-neoplastic tissue (P < 0.001). There was also a statistically significant correlation between the immunoexpression of E6 (P = 0.012) and E7 (P < 0.001) oncoproteins in lesion persistence among HIV-positive women. Within the limitations of this study, the immunoexpression of HPV 16/18 E6 and E7 oncoproteins may have prognostic value regarding lesion persistence in HIV-positive women.
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: L C
authors.initials: LC
authors.lastname: Rodrigues
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: N M de Gois
authors.initials: NM
authors.lastname: Speck
authors.affiliation: Departamento de Patologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: G R de Azevedo
authors.initials: GR
authors.lastname: Focchi
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Schimidt
authors.affiliation: Disciplina de Oncologia Ginecológica, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: R M
authors.initials: RM
authors.lastname: Marques
authors.affiliation: Núcleo de Prevenção de Doenças Ginecológicas, Departamento de Ginecologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors.forename: J C Lascasas
authors.initials: JC
authors.lastname: Ribalta
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D060085:Coinfection; D005260:Female; D015967:Gene Expression Regulation, Viral; D006678:HIV / Q000254:growth & development; D015658:HIV Infections / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000821:virology; D052162:Human papillomavirus 16 / Q000235:genetics / Q000254:growth & development; D052161:Human papillomavirus 18 / Q000235:genetics / Q000254:growth & development; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D060787:Neoplasm Grading; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics*; D050725:Papillomavirus E7 Proteins / Q000096:biosynthesis / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000821:virology; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000821:virology; D046888:Tissue Array Analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology* / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins