title: Protective mechanisms of wogonoside against Lipopolysaccharide/D-galactosamine-induced acute liver injury in mice.
abstract: Wogonoside, a bioactive flavonoid extracted from the root of Scutellaria baicalensis Georgi, has been reported to have anti-inflammatory and antioxidant effects. In this study, we examined the protective effects of wogonoside against lipopolysaccharide (LPS) and D-galactosamine (D-GalN)-induced liver injury in mice. Mice were given an intraperitoneal injection of wogonoside 1h before LPS and d-GalN treatment. The results showed that wogonoside inhibited the production of serum Alanine transaminase (ALT), Aspartate aminotransferase (AST), IL-1β, TNF-α, and hepatic malondialdehyde (MDA) content induced by LPS/GalN. In addition, wogonoside promoted the expression of Nrf2, NQO-1, GCLC, and HO-1. Wogonoside inhibited the expression of hepatic NLRP3, ASC, caspase-1, and IL-1β induced by LPS/GalN. In conclusion, these results suggest that wogonoside protects against LPS/GalN-induced acute liver injury by activating Nrf2 and inhibiting NLRP3 inflammasome activation.
authors.affiliation: Department of Infectious Disease, Binzhou Central Hospital Affiliated to Binzhou Medical University, Huanchengnan Road 108#, Binzhou, Shandong 251700, PR China. Electronic address: bcgyz@126.com.
authors.forename: Yuan-Zheng
authors.initials: YZ
authors.lastname: Gao
authors.affiliation: Department of Infectious Disease, Binzhou Central Hospital Affiliated to Binzhou Medical University, Huanchengnan Road 108#, Binzhou, Shandong 251700, PR China.
authors.forename: Lian-Feng
authors.initials: LF
authors.lastname: Zhao
authors.affiliation: Department of Infectious Disease, Binzhou Central Hospital Affiliated to Binzhou Medical University, Huanchengnan Road 108#, Binzhou, Shandong 251700, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Infectious Disease, Binzhou Central Hospital Affiliated to Binzhou Medical University, Huanchengnan Road 108#, Binzhou, Shandong 251700, PR China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Xue
authors.affiliation: Department of Infectious Disease, Binzhou Central Hospital Affiliated to Binzhou Medical University, Huanchengnan Road 108#, Binzhou, Shandong 251700, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D019610:Cytoprotection / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology*; D005688:Galactosamine / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D053583:Interleukin-1beta / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D044950:Flavanones; D005960:Glucosides; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D005688:Galactosamine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C473995:wogonoside; D005978:Glutathione
keywords: LPSNrf2; NLRP3; Wogonoside