title: Allograft inflammatory factor-1 in the pathogenesis of bleomycin-induced acute lung injury.
abstract: Allograft inflammatory factor-1 (AIF-1) is a protein expressed by macrophages infiltrating the area around the coronary arteries of rats with an ectopic cardiac allograft. Some studies have shown that expression of AIF-1 increased in a mouse model of trinitrobenzene sulfonic acid-induced acute colitis and in acute cellular rejection of human cardiac allografts. These results suggest that AIF-1 is related to acute inflammation. The current study used bleomycin-induced acute lung injury to analyze the expression of AIF-1 and to examine its function in acute lung injury. Results showed that AIF-1 was significantly expressed in lung macrophages and increased in bronchoalveolar lavage fluid from mice with bleomycin-induced acute lung injury in comparison to control mice. Recombinant AIF-1 increased the production of IL-6 and TNF-α from RAW264.7 (a mouse macrophage cell line) and primary lung fibroblasts, and it also increased the production of KC (CXCL1) from lung fibroblasts. These results suggest that AIF-1 plays an important role in the mechanism underlying acute lung injury.
authors.affiliation: Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine.
authors.forename: Hidetake
authors.initials: H
authors.lastname: Nagahara
authors.forename: Aihiro
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Seno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Obayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kida
authors.forename: Amane
authors.initials: A
authors.lastname: Nakabayashi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kukida
authors.forename: Kazuki
authors.initials: K
authors.lastname: Fujioka
authors.forename: Wataru
authors.initials: W
authors.lastname: Fujii
authors.forename: Ken
authors.initials: K
authors.lastname: Murakami
authors.forename: Masataka
authors.initials: M
authors.lastname: Kohno
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kawahito
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001761:Bleomycin; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D054360:Chemokine CXCL1 / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000150:complications / Q000473:pathology; D015850:Interleukin-6 / Q000378:metabolism; D008168:Lung / Q000473:pathology; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism*; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D054360:Chemokine CXCL1; D015850:Interleukin-6; D008840:Microfilament Proteins; D014409:Tumor Necrosis Factor-alpha; D001761:Bleomycin