title: Analysis of association of clinical aspects and IL1B tagSNPs with severe preeclampsia.
abstract: OBJECTIVE
  This study investigates the association between IL1B genotypes using a tag SNP (single polymorphism) approach, maternal and environmental factors in Brazilian women with severe preeclampsia.
  
  
  METHODS
  A case-control study with a total of 456 patients (169 preeclamptic women and 287 controls) was conducted in the two reference maternity hospitals of Sergipe state, Northeast Brazil. A questionnaire was administered and DNA was extracted to genotype the population for four tag SNPs of the IL1Beta: rs 1143643, rs 1143633, rs 1143634 and rs 1143630. Haplotype association analysis and p-values were calculated using the THESIAS test. Odds ratio (OR) estimation, confidence interval (CI) and multivariate logistic regression were performed.
  
  
  RESULTS
  High pregestational body mass index (pre-BMI), first gestation, cesarean section, more than six medical visits, low level of consciousness on admission and TC and TT genotype in rs1143630 of IL1Beta showed association with the preeclamptic group in univariate analysis. After multivariate logistic regression pre-BMI, first gestation and low level of consciousness on admission remained associated.
  
  
  CONCLUSIONS
  We identified an association between clinical variables and preeclampsia. Univariate analysis suggested that inflammatory process-related genes, such as IL1B, may be involved and should be targeted in further studies. The identification of the genetic background involved in preeclampsia host response modulation is mandatory in order to understand the preeclampsia process.
authors.affiliation: a Department of Medicine , Federal University of Sergipe , Farolândia , Aracaju , Brazil.
authors.forename: Larissa Paes
authors.initials: LP
authors.lastname: Leme Galvão
authors.affiliation: a Department of Medicine , Federal University of Sergipe , Farolândia , Aracaju , Brazil.
authors.forename: Filipe Emanuel
authors.initials: FE
authors.lastname: Menezes
authors.affiliation: a Department of Medicine , Federal University of Sergipe , Farolândia , Aracaju , Brazil.
authors.forename: Caio
authors.initials: C
authors.lastname: Mendonca
authors.affiliation: a Department of Medicine , Federal University of Sergipe , Farolândia , Aracaju , Brazil.
authors.forename: Ikaro
authors.initials: I
authors.lastname: Barreto
authors.affiliation: a Department of Medicine , Federal University of Sergipe , Farolândia , Aracaju , Brazil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Alvim-Pereira
authors.affiliation: a Department of Medicine , Federal University of Sergipe , Farolândia , Aracaju , Brazil.
authors.forename: Fabiano
authors.initials: F
authors.lastname: Alvim-Pereira
authors.affiliation: a Department of Medicine , Federal University of Sergipe , Farolândia , Aracaju , Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gurgel
mesh_terms: D000328:Adult; D015992:Body Mass Index; D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000235:genetics*; D011247:Pregnancy; D012720:Severity of Illness Index
chemical_list: D053583:Interleukin-1beta
keywords: Case-control study; maternal morbidity and mortality; molecular biology; polymorphisms; preeclampsia