title: Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression.
abstract: BACKGROUND
  Bone marrow mesenchymal stem cell (BMSCs)-based therapy seems to be a promising treatment for acute lung injury, but the therapeutic effects of BMSCs transplantation on acute lung injury induced by brain ischemia and the mechanisms have not been totally elucidated. This study explores the effects of transplantation of BMSCs on acute lung injury induced by focal cerebral ischemia and investigates the underlying mechanism.
  
  
  METHODS
  Acute lung injury model was induced by middle cerebral artery occlusion (MCAO). BMSCs (with concentration of 1 × 10(6)/ml) were transplanted into host through tail vein 1 day after MCAO. Then, the survival, proliferation and migration of BMSCs in lung were observed at 4 days after transplantation, and histology observation and lung function were assessed for 7 days. Meanwhile, in situ hybridization (ISH), qRT-PCR and western blotting were employed to detect the expression of TNF-α in lung.
  
  
  RESULTS
  Neurobehavioral deficits and acute lung injury could be seen in brain ischemia rats. Implanted BMSCs could survive in the lung, and relieve pulmonary edema, improve lung function, as well as down regulate TNF-α expression.
  
  
  CONCLUSIONS
  The grafted BMSCs can survive and migrate widespread in lung and ameliorate lung injury induced by focal cerebral ischemia in the MCAO rat models. The underlying molecular mechanism, at least partially, is related to the suppression of TNF-α.
authors.affiliation: Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. 851794319@qq.com.
authors.forename: Qin-qin
authors.initials: QQ
authors.lastname: He
authors.affiliation: Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. 550710080@qq.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.affiliation: Institute of Neuroscience and Experiment Animal Center, Kunming Medical University, Kunming, 650031, China. 441793506@qq.com.
authors.forename: Yan-ping
authors.initials: YP
authors.lastname: Wang
authors.affiliation: Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. 2016502193@qq.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. 648278976@qq.com.
authors.forename: Qing-jie
authors.initials: QJ
authors.lastname: Xia
authors.affiliation: Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. 499465010@qq.com.
authors.forename: Liu-Lin
authors.initials: LL
authors.lastname: Xiong
authors.affiliation: Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. chaozhil@aliyun.com.
authors.forename: Chao-zhi
authors.initials: CZ
authors.lastname: Luo
authors.affiliation: Center for Experimental Technology for Preclinical Medicine, Chengdu Medical College, Chengdu, 610083, Sichuan, China. woodwind@sohu.com.
authors.forename: Xiao-song
authors.initials: XS
authors.lastname: Hu
authors.affiliation: Institute of Neuroscience and Experiment Animal Center, Kunming Medical University, Kunming, 650031, China. liujiaaixuexi@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. tinghua_neuron@263.net.|Institute of Neuroscience and Experiment Animal Center, Kunming Medical University, Kunming, 650031, China. tinghua_neuron@263.net.
authors.forename: Ting-hua
authors.initials: TH
authors.lastname: Wang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism; D055370:Lung Injury / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D014409:Tumor Necrosis Factor-alpha