title: The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation.
abstract: Meiotic recombination is induced by the formation of DNA double-strand breaks (DSBs) catalyzed by SPO11, the ortholog of subunit A of TopoVI DNA topoisomerase (TopoVIA). TopoVI activity requires the interaction between A and B subunits. We identified a conserved family of plant and animal proteins [the TOPOVIB-Like (TOPOVIBL) family] that share strong structural similarity to the TopoVIB subunit of TopoVI DNA topoisomerase. We further characterize the meiotic recombination proteins Rec102 (Saccharomyces cerevisiae), Rec6 (Schizosaccharomyces pombe), and MEI-P22 (Drosophila melanogaster) as homologs to the transducer domain of TopoVIB. We demonstrate that the mouse TOPOVIBL protein interacts and forms a complex with SPO11 and is required for meiotic DSB formation. We conclude that meiotic DSBs are catalyzed by a complex involving SPO11 and TOPOVIBL.
authors.affiliation: Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France.
authors.forename: T
authors.initials: T
authors.lastname: Robert
authors.affiliation: Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France.
authors.forename: A
authors.initials: A
authors.lastname: Nore
authors.affiliation: Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France.
authors.forename: C
authors.initials: C
authors.lastname: Brun
authors.affiliation: Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France.
authors.forename: C
authors.initials: C
authors.lastname: Maffre
authors.affiliation: Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France.
authors.forename: B
authors.initials: B
authors.lastname: Crimi
authors.affiliation: Centre de Biologie du Développement, Université Fédérale de Toulouse, Paul Sabatier Campus, 118 Route de Narbonne, 31062 Toulouse, France. bernard.de-massy@igh.cnrs.fr henri-marc.bourbon@univ-tlse3.fr.
authors.forename: H-M
authors.initials: HM
authors.lastname: Bourbon
authors.affiliation: Institute of Human Genetics, UPR 1142 CNRS, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France. bernard.de-massy@igh.cnrs.fr henri-marc.bourbon@univ-tlse3.fr.
authors.forename: B
authors.initials: B
authors.lastname: de Massy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019843:Archaeal Proteins / Q000737:chemistry*; D002384:Catalysis; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry; D017124:Conserved Sequence; D053903:DNA Breaks, Double-Stranded; D004250:DNA Topoisomerases, Type II / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry; D029721:Drosophila Proteins / Q000737:chemistry; D004706:Endodeoxyribonucleases / Q000737:chemistry*; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D045522:Recombinases / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry; D016415:Sequence Alignment; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D019843:Archaeal Proteins; D002868:Chromosomal Proteins, Non-Histone; D004250:DNA Topoisomerases, Type II; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004706:Endodeoxyribonucleases; D010940:Plant Proteins; D045522:Recombinases; D029701:Saccharomyces cerevisiae Proteins; D000099257:Meiotic Recombination Protein SPO11; C517206:Mei-P22 protein, Drosophila; C073372:REC102 protein, S cerevisiae; C105141:DNA topoisomerase VI