title: [Clinical Significance of Detecting CyclinD1 and BCL-2 in Patients with B-Cell Lymphoma by Using Flow Cytometry].
abstract: OBJECTIVE
  To explore the feasibility and value of detecting CyclinD1 and BCL-2 in patients with B-cell lymphoma by using flow cytometry.
  
  
  METHODS
  Fifty-three patients with lymphoma were selected, and 50 healthy persons in the same period were selected as control. The expression levels of CyclinD1 and BCL-2 in patients with various subtypes of lymphoma were detected by using flow cytometry (FCM).
  
  
  RESULTS
  When the dilution time was 1 min and the dilution proportion was 1:20, the cell morphology was the best complete, at the 4 min the cell morphology was best status. The mean fluorescence intensity of CyclinD1 and BCL-2 between persons of control group and patients with B-cell lymphoma showed significant difference, the CyclinD1 level (1.824 ± 0.315) and BCL-2 levels (4.257 ± 0.528) of patients with B-cell lymphoma were obviously higher than the CyclinD1 level (0.634 ± 0.153) and BCL-2 level (1.926 ± 0.328) of persons in control group, the CyclinD1 and BCL-2 expression levels of patients with HL were significantly lower than CyclinD1 and BCL-2 levels of patients with NHL (P < 0.01). After treatment, the expression levels of CyclinD1 and BCL-2 in patients with B lymphoma were significantly lower than these befor treatment.
  
  
  CONCLUSIONS
  Using the method of flow cytometry for detecting CyclinD1 and BCL-2 expression levels in lymphoma cells of patients is feasible, and it can be applied clinically to evaluate the treatment efficacy.
authors.affiliation: Department of Clinical Laboratorial Examination, Huangdao Traditional Chinese Medicine Hospital, Qingdao 266500, Shandong Province, China. E-mail: 4799414014@qq.com.
authors.forename: Yan-Xiang
authors.initials: YX
authors.lastname: Cui
authors.affiliation: Department of Clinical Laboratory Examination, Yishui Center Hospital, Linyi 276400, Shandong Province, China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratorial Examination, Huangdao Traditional Chinese Medicine Hospital, Qingdao 266500, Shandong Province, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Chen
mesh_terms: D016022:Case-Control Studies; D019938:Cyclin D1 / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
chemical_list: C000595707:BCL2 protein, human; C530104:CCND1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D019938:Cyclin D1