title: A novel long non-coding RNA CYP4B1-PS1-001 regulates proliferation and fibrosis in diabetic nephropathy.
abstract: Diabetic nephropathy is an important microvascular complication of diabetes, and the incidence of end-stage renal disease caused by it are rising annually. Long non-coding RNAs (lncRNAs) are widely regarded to associate with the occurrence and development of various diseases; however, the relationship between lncRNAs and diabetic nephropathy remains largely unknown. This work studied the effect of lncRNAs on diabetic nephropathy pathogenesis. LncRNA microarrays were initially used to detect lncRNAs with altered expression in three cases of kidney tissue from db/db mice with diabetic nephropathy. LncRNAs with differential expression (>2-fold) could be considered candidates. Particularly, CYP4B1-PS1-001 was significantly downregulated in response to early diabetic nephropathy in vitro and in vivo, while overexpression of CYP4B1-PS1-001 inhibited proliferation and fibrosis of mesangial cells. Overall, our data indicate the potential role of CYP4B1-PS1-001 in the proliferation and fibrosis of mice mesangial cells as the prominent features during early stage of diabetic nephropathy, which extend the relationship between lncRNAs and diabetic nephropathy, and may provide a potential therapeutic target and molecular biomarker for the disease.
authors.affiliation: Department of Endocrinology and Metabolism, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Endocrinology and Metabolism, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, PR China.
authors.forename: Suyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Endocrinology and Metabolism, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, PR China.
authors.forename: Di
authors.initials: D
authors.lastname: Yao
authors.affiliation: Department of Microbiology, Nanjing Medical University, Nanjing 210029, PR China. Electronic address: yanqin@njmu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Department of Endocrinology and Metabolism, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, PR China. Electronic address: hyhalwp@sina.com.
authors.forename: Weiping
authors.initials: W
authors.lastname: Lu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D003928:Diabetic Nephropathies / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005355:Fibrosis; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D007668:Kidney / Q000473:pathology*; D008297:Male; D008810:Mice, Inbred C57BL; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000502:physiology*
chemical_list: D062085:RNA, Long Noncoding; C000613076:long non-coding RNA CYP4B1-PS1-001, mouse
keywords: Diabetic nephropathy; Fibrosis; Proliferation; lncRNA