title: Description of three novel Lagenidium (Oomycota) species causing infection in mammals.
abstract: BACKGROUND
  Recent molecular phylogenetic analysis of Lagenidium strains recovered from subcutaneous lesions in cats, dogs, and a human with lagenidiosis resolved into four clades; one of them was Lagenidium giganteum, but three others were novel.
  
  
  OBJECTIVE
  Due to the recent increase in L. giganteum infections from mammals, we studied 21 Lagenidium strains isolated from dogs and a human available in our collection.
  
  
  METHODS
  Molecular phylogenetic studies and phenotypic characteristics were used to characterize the strains.
  
  
  RESULTS
  We report the finding of three novel species, herein designated as Lagenidium ajelloi, sp. nov., Lagenidium albertoi sp. nov, and Lagenidium vilelae sp. nov. Their morphological and growth features are also presented.
  
  
  CONCLUSIONS
  Our study revealed the presence of three novel Lagenidium species infecting mammals.
authors.affiliation: Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States; Biomedical Laboratory Diagnostics, Michigan State University, East Lansing, MI 48824, United States. Electronic address: mendoza9@msu.edu.
authors.forename: Leonel
authors.initials: L
authors.lastname: Mendoza
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States.
authors.forename: John W
authors.initials: JW
authors.lastname: Taylor
authors.affiliation: Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States.
authors.forename: Edward D
authors.initials: ED
authors.lastname: Walker
authors.affiliation: Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States; Faculty of Pharmacy, Universidade Federal do Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Vilela
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004283:Dog Diseases / Q000209:etiology*; D004285:Dogs; D006801:Humans; D007634:Keratitis / Q000209:etiology*; D044726:Lagenidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D010802:Phylogeny; D012874:Skin Diseases, Infectious / Q000209:etiology / Q000662:veterinary*; D013045:Species Specificity; D040521:Subcutaneous Tissue
chemical_list: D004247:DNA
keywords: Lagenidiosis; Lagenidium; Oomycetes; Oomycota; Peronosporomycetes; Pythium insidiosum; Straminipila