title: Evaluation of Parameters for High Efficiency Transformation of Acinetobacter baumannii.
abstract: Acinetobacter baumannii is an emerging, nosocomial pathogen that is poorly characterized due to a paucity of genetic tools and methods. While whole genome sequence data from several epidemic and environmental strains have recently become available, the functional characterization of genes is significantly lagging. Efficient transformation is one of the first steps to develop molecular tools that can be used to address these shortcomings. Here we report parameters allowing high efficiency transformation of A. baumannii. Using a multi-factorial experimental design we found that growth phase, voltage, and resistance all significantly contribute to transformation efficiency. The highest efficiency (4.3 × 10(8) Transformants/μg DNA) was obtained at the stationary growth phase of the bacterium (OD 6.0) using 25 ng of plasmid DNA under 100 Ohms resistance and 1.7 kV/cm voltage. The optimized electroporation parameters reported here provide a useful tool for genetic manipulation of A. baumannii.
authors.affiliation: Department of Wound Infections, Bacterial Diseases Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Suleyman
authors.initials: S
authors.lastname: Yildirim
authors.affiliation: Department of Wound Infections, Bacterial Diseases Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Mitchell G
authors.initials: MG
authors.lastname: Thompson
authors.affiliation: Department of Wound Infections, Bacterial Diseases Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Jacobs
authors.affiliation: Department of Wound Infections, Bacterial Diseases Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Zurawski
authors.affiliation: Department of Wound Infections, Bacterial Diseases Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.|Department of Medicine, Infectious Diseases Division, Uniformed Services University of the Health Sciences, F. Edward Hebert School of Medicine, Bethesda, Maryland, USA.
authors.forename: Benjamin C
authors.initials: BC
authors.lastname: Kirkup
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial