title: Genome-wide DNA methylation profile of developing deciduous tooth germ in miniature pigs.
abstract: BACKGROUND
  DNA methylation is an important epigenetic modification critical to the regulation of gene expression during development. To date, little is known about the role of DNA methylation in tooth development in large animal models. Thus, we carried out a comparative genomic analysis of genome-wide DNA methylation profiles in E50 and E60 tooth germ from miniature pigs using methylated DNA immunoprecipitation-sequencing (MeDIP-seq).
  
  
  RESULTS
  We observed different DNA methylation patterns during the different developmental stages of pig tooth germ. A total of 2469 differentially methylated genes were identified. Functional analysis identified several signaling pathways and 104 genes that may be potential key regulators of pig tooth development from E50 to E60.
  
  
  CONCLUSIONS
  The present study provided a comprehensive analysis of the global DNA methylation pattern of tooth germ in miniature pigs and identified candidate genes that potentially regulate tooth development from E50 to E60.
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. reliayysu@163.com.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Su
authors.affiliation: Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. zpfan@ccmu.edu.cn.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. csuxywxs@foxmail.com.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Wu
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. liyanggood@gmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. dywangfu@163.com.
authors.forename: Fu
authors.initials: F
authors.lastname: Wang
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. kqjy55@163.com.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. wjs99330@163.com.|Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, You An Men Wai Xi Tou Tiao No.10, Beijing, 100069, China. wjs99330@163.com.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Physiology and Pathophysiology, Beijing An Zhen Hospital the Key Laboratory of Remodeling-Related Cardiovascular Diseases, School of Basic Medical Sciences, Capital Medical University, You An Men Wai Xi Tou Tiao No.10, Beijing, 100069, China. jdu@bcm.edu.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tian Tan Xi Li No.4, Beijing, 100050, China. slwang@ccmu.edu.cn.|Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, You An Men Wai Xi Tou Tiao No.10, Beijing, 100069, China. slwang@ccmu.edu.cn.
authors.forename: Songlin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D059014:High-Throughput Nucleotide Sequencing; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics*; D014083:Tooth Germ / Q000378:metabolism*; D014094:Tooth, Deciduous / Q000378:metabolism*