title: Interaction of Catechu Dye with DNA: Spectroscopic and In Silico Approach.
abstract: Catechin, a yellow colored molecule obtained from the wood of Acacia catechu was analyzed for its interaction with synthetic DNA duplexes using spectroscopic analysis. UV-Visible spectroscopic analysis revealed the non-intercalative binding mode. Fourier Transform Infrared spectroscopy (FTIR) analysis expose chemical shift indicated by various vibrational stretches and an increase in the intensity of base stacking was observed by Circular Dichroism (CD), respectively. This inference was further confirmed through nuclear staining technique and also in electrophoretic technique; the dye quenches the fluorescent intensity of ethidium bromide. The result of fluorescence spectroscopy was in concordance with the electrophoretic technique. In addition, the spectroscopic results were in accordance with the molecular docking studies of specific catechin compound from the catechu dye with CT-DNA. This kind of site specificity is a gain in the medicinal field as the drug can be DNA targeted for cancer therapeutics. The present work reveals that catechu dye has a noteworthy application in the field of medical bioscience.
authors.affiliation: a School of Bio Sciences and Technology, VIT University , Vellore , 632014 , Tamil Nadu , India.
authors.forename: Hridya
authors.initials: H
authors.lastname: Hemachandran
authors.affiliation: a School of Bio Sciences and Technology, VIT University , Vellore , 632014 , Tamil Nadu , India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Anantharaman
authors.affiliation: a School of Bio Sciences and Technology, VIT University , Vellore , 632014 , Tamil Nadu , India.
authors.forename: Rajendra Rao
authors.initials: RR
authors.lastname: Priya
authors.affiliation: a School of Bio Sciences and Technology, VIT University , Vellore , 632014 , Tamil Nadu , India.
authors.forename: George Priya
authors.initials: GP
authors.lastname: Doss
authors.affiliation: a School of Bio Sciences and Technology, VIT University , Vellore , 632014 , Tamil Nadu , India.
authors.forename: Ramamoorthy
authors.initials: R
authors.lastname: Siva
mesh_terms: D002392:Catechin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D062105:Molecular Docking Simulation; D013057:Spectrum Analysis
chemical_list: D002392:Catechin; D004247:DNA; C427203:calf thymus DNA
keywords: Catechin; DNA; acacia catechu; interaction; natural dyes; non-intercalation