title: A modified micro chamber agar spot slide culture technique for microscopic examination of filamentous fungi.
abstract: The slide culture technique aids in the study of undisturbed microscopic morphological details of filamentous fungi. The existing methods for setting up of slide culture are quite cumbersome, time-consuming and require elaborate preparation. We describe a modified and easy to perform micro chamber agar spot slide culture technique.
authors.affiliation: Medical Mycology Laboratory, Department of Microbiology, II floor, Centre for Basic Sciences, Kasturba Medical College - Manipal, Manipal University, Madhav Nagar, Manipal 576 104, Karnataka, India. Electronic address: prakashpy123@yahoo.co.in.
authors.forename: Peralam Yegneswaran
authors.initials: PY
authors.lastname: Prakash
authors.affiliation: Department of Microbiology, II floor, Centre for Basic Sciences, Kasturba Medical College - Manipal, Manipal University, Madhav Nagar, Manipal 576 104, Karnataka, India. Electronic address: kanikabhargava22@gmail.com.
authors.forename: Kanika
authors.initials: K
authors.lastname: Bhargava
mesh_terms: D000362:Agar; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005658:Fungi / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*
chemical_list: D003470:Culture Media; D000362:Agar
keywords: Agar spot; Microscopy of fungus; Microscopy of mould; Slide culture technique