title: Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments.
abstract: Exopolysaccharides (EPS) are polymers excreted by some microorganisms with interesting properties and used in many industrial applications. A new Pseudoalteromonas sp. strain, MD12-642, was isolated from marine sediments and cultivated in bioreactor in saline culture medium containing glucose as carbon source. Its ability to produce EPS under saline conditions was demonstrated reaching an EPS production of 4.4g/L within 17hours of cultivation, corresponding to a volumetric productivity of 0.25g/Lh, the highest value so far obtained for Pseudoalteromonas sp. strains. The compositional analysis of the EPS revealed the presence of galacturonic acid (41-42mol%), glucuronic acid (25-26mol%), rhamnose (16-22mol%) and glucosamine (12-16mol%) sugar residues. The polymer presents a high molecular weight (above 1000kDa). These results encourage the biotechnological exploitation of strain MD12-642 for the production of valuable EPS with unique composition, using saline by-products/wastes as feedstocks.
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal. Electronic address: christophe.roca@fct.unl.pt.
authors.forename: Christophe
authors.initials: C
authors.lastname: Roca
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Mareen
authors.initials: M
authors.lastname: Lehmann
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Cristiana A V
authors.initials: CA
authors.lastname: Torres
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Sílvia
authors.initials: S
authors.lastname: Baptista
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal; LAQV-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Susana P
authors.initials: SP
authors.lastname: Gaudêncio
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Filomena
authors.initials: F
authors.lastname: Freitas
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Reis
mesh_terms: D001267:Atlantic Ocean; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D019015:Geologic Sediments / Q000382:microbiology*; D008970:Molecular Weight; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D011174:Portugal; D031161:Pseudoalteromonas / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial