title: Aqueous Mesocosm Techniques Enabling the Real-Time Measurement of the Chemical and Isotopic Kinetics of Dissolved Methane and Carbon Dioxide.
abstract: Previous studies of microbially mediated methane oxidation in oceanic environments have examined the many different factors that control the rates of oxidation. However, there is debate on what factor(s) are limiting in these types of environments. These factors include the availability of methane, O2, trace metals, nutrients, the density of cell population, and the influence that CO2 production may have on pH. To look at this process in its entirety, we developed an automated mesocosm incubation system with a Dissolved Gas Analysis System (DGAS) coupled to a myriad of analytical tools to monitor chemical changes during methane oxidation. Here, we present new high temporal resolution techniques for investigating dissolved methane and carbon dioxide concentrations and stable isotopic dynamics during aqueous mesocosm and pure culture incubations. These techniques enable us to analyze the gases dissolved in solution and are nondestructive to both the liquid media and the analyzed gases enabling the investigation of a mesocosm or pure culture experiment in a completely closed system, if so desired.
authors.affiliation: Department of Earth and Environmental Sciences, University of Rochester , Rochester, New York 14627, United States.
authors.forename: Eric W
authors.initials: EW
authors.lastname: Chan
authors.affiliation: Department of Earth and Environmental Sciences, University of Rochester , Rochester, New York 14627, United States.
authors.forename: John D
authors.initials: JD
authors.lastname: Kessler
authors.affiliation: Department of Marine Science, University of Southern Mississippi , Stennis Space Center, Mississippi 39529, United States.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Shiller
authors.affiliation: Department of Marine Science, University of Southern Mississippi , Stennis Space Center, Mississippi 39529, United States.|Vermont EPSCoR, University of Vermont Burlington, Vermont 05405, United States.
authors.forename: DongJoo
authors.initials: D
authors.lastname: Joung
authors.affiliation: Pactech Packaging, LLC , Rochester, New York 14624, United States.
authors.forename: Frank
authors.initials: F
authors.lastname: Colombo
mesh_terms: D002245:Carbon Dioxide / Q000032:analysis*; D002247:Carbon Isotopes / Q000032:analysis; D004463:Ecology / Q000295:instrumentation / Q000379:methods*; D005740:Gases / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D008670:Metals / Q000032:analysis; D008697:Methane / Q000032:analysis*; D009792:Oceans and Seas; D010084:Oxidation-Reduction; D014867:Water / Q000032:analysis / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D005740:Gases; D008670:Metals; D014867:Water; D002245:Carbon Dioxide; D008697:Methane