title: Assembly of HE800 exopolysaccharide produced by a deep-sea hydrothermal bacterium into microgels for protein delivery applications.
abstract: Assembly of biopolymers into microgels is an elegant strategy for bioencapsulation with various potential biomedical applications. Such biocompatible and biodegradable microassemblies are developed not only to protect the encapsulated molecule but also to ensure its sustained local delivery. The present study describes the fabrication of microassemblies from a marine HE800 exopolysaccharide (EPS), which displays a glycosaminoglycan (GAG)-like structure and biological properties. HE800 EPS was assembled, through physical cross-linking with divalent ions, into microgel particles and microfibers using microfluidics. The microparticle morphology was highly affected by the polysaccharide concentration and its molecular weight. A model protein, namely Bovine Serum Albumin (BSA) was subsequently encapsulated within HE800 microparticles in one-step process using microfluidics. The protein release was tuned by the microparticle morphology with a lower protein amount released from the most homogeneous structures. Our findings demonstrate the high potential of HE800 EPS based microassemblies as innovative protein microcarriers for further biomedical applications.
authors.affiliation: Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, 44311 Nantes, France. Electronic address: Agata.Zykwinska@ifremer.fr.
authors.forename: Agata
authors.initials: A
authors.lastname: Zykwinska
authors.affiliation: INRA, UR1268 Biopolymères Interactions Assemblages, F-44300 Nantes, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Marquis
authors.affiliation: Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, 44311 Nantes, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Sinquin
authors.affiliation: Institut des Matériaux Jean Rouxel (IMN), Université de Nantes-CNRS, 44322 Nantes, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Cuenot
authors.affiliation: Ifremer, Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies, 44311 Nantes, France.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Colliec-Jouault
mesh_terms: D000818:Animals; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D011077:Polychaeta / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry; D014733:Vibrio / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D005782:Gels; D006025:Glycosaminoglycans; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine
keywords: Marine exopolysaccharide; Microencapsulation; Microfibers; Microfluidic; Microgel particles; Protein release