title: Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in Bradyrhizobium Diazoefficiens.
abstract: The hydrogen uptake (Hup) system of Bradyrhizobium diazoefficiens recycles the H2 released by nitrogenase in soybean nodule symbiosis, and is responsible for H2-dependent chemolithoautotrophic growth. The strain USDA110 has two hup gene clusters located outside (locus I) and inside (locus II) a symbiosis island. Bacterial growth under H2-dependent chemolithoautotrophic conditions was markedly weaker and H2 production by soybean nodules was markedly stronger for the mutant of hup locus I (ΔhupS1L1) than for the mutant of hup locus II (ΔhupS2L2). These results indicate that locus I is primarily responsible for Hup activity.
authors.affiliation: Graduate School of Life Sciences, Tohoku University.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Masuda
authors.forename: Masaki
authors.initials: M
authors.lastname: Saito
authors.forename: Chiaki
authors.initials: C
authors.lastname: Sugawara
authors.forename: Manabu
authors.initials: M
authors.lastname: Itakura
authors.forename: Shima
authors.initials: S
authors.lastname: Eda
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Minamisawa
mesh_terms: D020369:Bradyrhizobium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D052837:Chemoautotrophic Growth; D055786:Gene Knockout Techniques; D044404:Genomic Islands; D006859:Hydrogen / Q000378:metabolism*; D005810:Multigene Family; D053204:Root Nodules, Plant / Q000382:microbiology; D013025:Glycine max / Q000382:microbiology; D013559:Symbiosis
chemical_list: D006859:Hydrogen