title: Growth promotion in pigs by oxytetracycline coincides with down regulation of serum inflammatory parameters and of hibernation-associated protein HP-27.
abstract: The growth promoting effect of supplementing animal feed with antibiotics like tetracycline has traditionally been attributed to their antibiotic character. However, more evidence has been accumulated on their direct anti-inflammatory effect during the last two decades. Here we used a pig model to explore the systemic molecular effect of feed supplementation with sub therapeutic levels of oxytetracycline (OTC) by analysis of serum proteome changes. Results showed that OTC promoted growth, coinciding with a significant down regulation of different serum proteins related to inflammation, oxidation and lipid metabolism, confirming the anti-inflammatory mechanism of OTC. Interestingly, apart from the classic acute phase reactants also down regulation was seen of a hibernation associated plasma protein (HP-27), which is to our knowledge the first description in pigs. Although the exact function in non-hibernators is unclear, down regulation of HP-27 could be consistent with increased appetite, which is possibly linked to the anti-inflammatory action of OTC. Given that pigs are good models for human medicine due to their genetic and physiologic resemblance, the present results might also be used for rational intervention in human diseases in which inflammation plays an important role such as obesity, type 2 diabetes and cardiovascular diseases.
authors.affiliation: Livestock-Nutrition-Quality Division, Department of Biosystems, Faculty of Biosciences Engineering, KU Leuven, Heverlee, Belgium.
authors.forename: Laura
authors.initials: L
authors.lastname: Soler
authors.affiliation: Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Miller
authors.affiliation: VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Karin
authors.initials: K
authors.lastname: Hummel
authors.affiliation: VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Razzazi-Fazeli
authors.affiliation: Division of Industrial Food Research, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
authors.forename: Flemming
authors.initials: F
authors.lastname: Jessen
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, Murcia, Spain.
authors.forename: Damian
authors.initials: D
authors.lastname: Escribano
authors.affiliation: Livestock-Nutrition-Quality Division, Department of Biosystems, Faculty of Biosciences Engineering, KU Leuven, Heverlee, Belgium.
authors.forename: Theo
authors.initials: T
authors.lastname: Niewold
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis*; D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D019587:Dietary Supplements; D015536:Down-Regulation / Q000187:drug effects*; D004586:Electrophoresis; D006023:Glycoproteins / Q000097:blood*; D006242:Haptoglobins / Q000032:analysis; D006605:Hibernation; D010118:Oxytetracycline / Q000008:administration & dosage*; D000685:Serum Amyloid A Protein / Q000032:analysis; D013552:Swine / Q000254:growth & development*; D053719:Tandem Mass Spectrometry
chemical_list: D000209:Acute-Phase Proteins; D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D006023:Glycoproteins; D006242:Haptoglobins; D000685:Serum Amyloid A Protein; C115605:hibernation-related protein, vertebrate; D010118:Oxytetracycline
keywords: Antimicrobial growth promoters; Hibernation-associated plasma protein-27; Immunometabolism; Oxytetracycline; Proteomics