title: [Effect of Rapamycin on Expression of Survivin and Caspase-3 and Its Influence on K562 Cell Ultrastructure].
abstract: OBJECTIVE
  To investigate the effect of rapamycin on the expression of survivin and caspase-3 at mRNA level in K562 cells and the influence of rapamycin on K562 cell ultrastructure.
  
  
  METHODS
  The effects of rapamycin at various concentration on K562 cell proliferation were analyzed by CCK8; the morphological characteristics of K562 cells was observed by transmission electron microscopy; the expression of survivin and caspase-3 at mRNA level in K562 cells treated with rapamycin was detected by RT-PCR.
  
  
  RESULTS
  The proliferation of K562 cells was significantly inhibited by rapamycin. The apoptosis level of K562 cells increased with increase of rapamycin concentration, the expression of survivin at mRNA level decreased with increase of rapamycin concentration (P < 0.05). The expression of caspase-3 at mRNA level increased with increase of rapamycin concentration.
  
  
  CONCLUSIONS
  Rapamycin can prornote K562 cell apoptosis through up-regulating caspase-3 level and reduceing survivin level.
authors.affiliation: Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Zi-Yuan
authors.initials: ZY
authors.lastname: Nie
authors.affiliation: Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Yin-Tao
authors.initials: YT
authors.lastname: Shan
authors.affiliation: Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Pan
authors.affiliation: Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. E-mail: luojm315@163.com.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Luo
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D049109:Cell Proliferation; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D020014:K562 Cells / Q000187:drug effects / Q000648:ultrastructure; D012333:RNA, Messenger; D020123:Sirolimus / Q000494:pharmacology*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin; C505407:CASP3 protein, human; D053148:Caspase 3; D020123:Sirolimus