title: Lumican Inhibits SNAIL-Induced Melanoma Cell Migration Specifically by Blocking MMP-14 Activity.
abstract: Lumican, a small leucine rich proteoglycan, inhibits MMP-14 activity and melanoma cell migration in vitro and in vivo. Snail triggers epithelial-mesenchymal transitions endowing epithelial cells with migratory and invasive properties during tumor progression. The aim of this work was to investigate lumican effects on MMP-14 activity and migration of Snail overexpressing B16F1 (Snail-B16F1) melanoma cells and HT-29 colon adenocarcinoma cells. Lumican inhibits the Snail induced MMP-14 activity in B16F1 but not in HT-29 cells. In Snail-B16F1 cells, lumican inhibits migration, growth, and melanoma primary tumor development. A lumican-based strategy targeting Snail-induced MMP-14 activity might be useful for melanoma treatment.
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.|Department of Cytobiology and Proteomics, Medical University of Lodz, Lodz, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Stasiak
authors.affiliation: Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Boncela
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Perreau
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.|Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras, Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Karamanou
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Chatron-Colliet
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Proult
authors.affiliation: Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Przygodzka
authors.affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States of America.
authors.forename: Shukti
authors.initials: S
authors.lastname: Chakravarti
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.|CHU de Reims, Laboratoire Central de Biochimie, Reims, France.
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Maquart
authors.affiliation: Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland.|Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, United States of America.
authors.forename: M Anna
authors.initials: MA
authors.lastname: Kowalska
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Yanusz
authors.initials: Y
authors.lastname: Wegrowski
authors.affiliation: CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et de Biologie Moléculaire, Reims, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Brézillon
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D011508:Chondroitin Sulfate Proteoglycans / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D007632:Keratan Sulfate / Q000494:pharmacology*; D000071234:Lumican; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; C000604306:LUM protein, human; D000071234:Lumican; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D007632:Keratan Sulfate; D053511:Matrix Metalloproteinase 14