title: Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders.
abstract: The activity of matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fluids (SF) sampled from dogs with joint disorders was investigated by gelatin zymography and densitometry. Pro-MMP-2 showed similar activity levels in dogs with idiopathic polyarthritis (IPA; n=17) or canine rheumatoid arthritis (cRA; n=4), and healthy controls (n=10). However, dogs with cranial cruciate ligament rupture (CCLR; n=5) presented significantly higher pro-MMP-2 activity than IPA and healthy dogs. Meanwhile, dogs with IPA exhibited significantly higher activity of pro- and active MMP-9 than other groups. Activity levels in pro- and active MMP-9 in cRA and CCLR dogs were not significantly different from those in healthy controls. Different patterns of MMP-2 and MMP-9 activity may reflect the differences in the underlying pathological processes.
authors.affiliation: Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Murakami
authors.forename: Shingo
authors.initials: S
authors.lastname: Maeda
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Naoaki
authors.initials: N
authors.lastname: Matsuki
mesh_terms: D000818:Animals; D001168:Arthritis / Q000201:enzymology / Q000662:veterinary; D001172:Arthritis, Rheumatoid / Q000201:enzymology / Q000662:veterinary; D016022:Case-Control Studies; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs / Q000293:injuries; D005260:Female; D007592:Joint Diseases / Q000201:enzymology / Q000662:veterinary*; D007596:Joints / Q000293:injuries; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D013582:Synovial Fluid / Q000201:enzymology*
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9