title: Evaluation of the effect of three supplementary oral hygiene measures on salivary mutans streptococci levels in children: A randomized comparative clinical trial.
abstract: OBJECTIVE
  The aim of this study was to evaluate and compare the effect of tongue scraping, tongue brushing, and saturated saline on salivary MS levels.
  
  
  METHODS
  A single-blinded, randomized, parallel group clinical trial was conducted in children aged 9-12 years. Total sample of 45 subjects were randomly assigned to three groups, that is, Group A, Group B, and Group C comprised of 15 each. Group A, Group B, and Group C were asked to do tongue scraping, tongue brushing and saturated saline rinsing twice daily, respectively for 21 days. Saliva samples, collected from the subjects on the baseline, 7(th) day and 21(st) day, were inoculated on mitis salivarius bacitracin agar and incubated at 37°C for 48 h. The mean streptococcal colony forming counts were enumerated. The data were subjected to statistical analysis using Wilcoxon signed rank sum test for intragroup comparisons and Mann-Whitney U-test for intergroup comparisons.
  
  
  RESULTS
  Intragroup comparisons showed statistically significant reduction in MS levels (P < 0.01). However, the intergroup comparisons showed no statistically significant difference (P > 0.05).
  
  
  CONCLUSIONS
  The oral hygiene measures evaluated proved equal efficacy in reducing the colony counts. Hence, there is a need to emphasize the importance of incorporating supplementary oral hygiene measures in daily oral care.
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Dr. Syamala Reddy Dental College Hospital and Research Center, Munnekolala, Bengaluru, Karnataka, India.
authors.forename: M
authors.initials: M
authors.lastname: Manju
authors.affiliation: Department of Pedodontics and Preventive Dentistry, C.K.S Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Prathyusha
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Pushpagiri College of Dental Sciences, Thiruvalla, Kerala, India.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Joseph
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Darshan Dental College and Hospital, Udaipur, Rajasthan, India.
authors.forename: Rupali Borkar
authors.initials: RB
authors.lastname: Kaul
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Coorg Institute of Dental Sciences, Virajpet, Karnataka, India.
authors.forename: Srinivas L
authors.initials: SL
authors.lastname: Shanthraj
authors.affiliation: Consultant Pediatric Dentist, Total Dental Care, Najafgarh, New Delhi, India.
authors.forename: Ntasha
authors.initials: N
authors.lastname: Sethi
keywords: Dental caries; mutans streptococci; oral hygiene; saliva