title: Application of HIF-1α by gene therapy enhances angiogenesis and osteogenesis in alveolar bone defect regeneration.
abstract: BACKGROUND
  A successful clinical outcome for implanted tissue-engineered bone is dependent on the establishment of a functional vascular network. Gene-enhanced tissue engineering represents a promising approach for vascularization and osteogenesis. In the present study, we tested the angiogenesis and osteogenesis efficacy of gelatin as the scaffold carrier in combination with a virus encoding the HIF-1α gene in a rat alveolar bone defect model.
  
  
  METHODS
  Three groups of 10 rats each were either left untreated, treated with adenovirus encoding hypoxia-inducible factor-1α (AdHIF-1α)/gelatin sponge or treated with gelatin sponge with adenovirus encoding red fluorescence protein, respectively. At 4 weeks, all samples were determined by micro-computed tomography, histological analyses and immunohistochemical studies.
  
  
  RESULTS
  Scaffolds loaded with AdHIF-1α were able to sustain the release of AdHIF-1α for up to 21 days and alveolar bone defects treated with scaffolds containing AdHIF-1α significantly induced new bone and new vessel formation in vivo.
  
  
  CONCLUSIONS
  Overexpression of HIF-1α by gene therapy may be a useful method for enhancing alveolar bone defect osteogenesis and angiogenesis. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.|Stomatological Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.|Stomatological Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Huang
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.|Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.|Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.|Stomatological Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Hu
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.|Stomatological Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Guangyue
authors.initials: G
authors.lastname: Li
authors.affiliation: Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.|Stomatological Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D016301:Alveolar Bone Loss / Q000235:genetics* / Q000503:physiopathology / Q000628:therapy; D000818:Animals; D001861:Bone Regeneration / Q000235:genetics*; D002478:Cells, Cultured; D015316:Genetic Therapy / Q000379:methods; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic / Q000235:genetics*; D010012:Osteogenesis / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D055114:X-Ray Microtomography
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit
keywords: HIF-1α; angiogenesis; bone defect; gene therapy; osteogenesis