title: Time-resolved proteomic analysis of quorum sensing inVibrio harveyi.
abstract: Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organismVibrio harveyiby bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition ofV. harveyifrom individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.
authors.forename: John D
authors.initials: JD
authors.lastname: Bagert
authors.affiliation: Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405.
authors.forename: Julia C
authors.initials: JC
authors.lastname: van Kessel
authors.affiliation: Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, CA 91125.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Sweredoski
authors.affiliation: Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63108.
authors.forename: Lihui
authors.initials: L
authors.lastname: Feng
authors.affiliation: Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, CA 91125.
authors.forename: Sonja
authors.initials: S
authors.lastname: Hess
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ 08544; Howard Hughes Medical Institute, Chevy Chase, MD 20815.
authors.forename: Bonnie L
authors.initials: BL
authors.lastname: Bassler
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.
authors.forename: David A
authors.initials: DA
authors.lastname: Tirrell