title: In vitro evaluation of aspirin-induced HspB1 against heat stress damage in chicken myocardial cells.
abstract: To understand the potential association of heat stress resistance with HspB1 induction by aspirin (ASA) in chicken myocardial cells, variations of HspB1 expression and heat stressed-induced damage of myocardial cells after ASA administration were studied in primary cultured myocardial cells. Cytopathological lesions as well as damage-related enzymes, such as creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH), indicated the considerable protective ability of ASA pre-treatment against acute heat stress. Immunostaining assays showed that heat stress caused HspB1 to relocate into the nucleus, while ASA did not. ELISA analysis, revealed that HspB1 expression induced by ASA averaged 45.62-fold higher than that of the control. These results indicated that the acute heat-stressed injuries were accompanied by comparatively lower HspB1 expression caused by heat stress in vitro. ASA pre-treatment induced a level of HspB1 presumed to be sufficient to protect myocardial cells from acute heat stress in the extracorporal model, although more detailed mechanisms will require further investigation.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Jinling Institute of Technology, Nanjing, 210038, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
authors.forename: Erbao
authors.initials: E
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
authors.forename: Yinjun
authors.initials: Y
authors.lastname: Lv
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behavior, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Kemper
authors.affiliation: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behavior, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hartung
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China. b_endong@njau.edu.cn.
authors.forename: Endong
authors.initials: E
authors.lastname: Bao
mesh_terms: D000818:Animals; D001241:Aspirin / Q000008:administration & dosage*; D002645:Chickens; D052279:Creatine Kinase, MB Form / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D018869:Heat-Shock Response / Q000187:drug effects*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D061251:Primary Cell Culture
chemical_list: D055551:HSP27 Heat-Shock Proteins; D007770:L-Lactate Dehydrogenase; D052279:Creatine Kinase, MB Form; D001241:Aspirin
keywords: Aspirin; Heat shock proteins; Heat stress; HspB1; In vitro; Myocardial cell protection