title: Effect of antibiotic on survival and development of Spodoptera litura (Lepidoptera: Noctuidae) and its gut microbial diversity.
abstract: Addition of antibiotics to artificial diets of insects is a key component in the rearing of insects in the laboratory. In the present study an antimicrobial agent, streptomycin sulphate was tested for its influence on survival and fitness of Spodoptera litura (Fabricus) (Lepidoptera: Noctuidae) as well as its gut microbial diversity. The antibiotic did not adversely affect the survival of S. litura. Faster growth of larvae was recorded on diet amended with different concentrations of streptomycin sulphate (0.03, 0.07 and 0.15%) as compared to diet without streptomycin sulphate. The overall activity of various digestives enzymes increased on S+ diet while the activity of detoxifying enzymes significantly decreased. In addition, alteration in microbial diversity was found in the gut of S. litura larvae fed on diet supplemented with antibiotic (S+) and without antibiotic (S-).
authors.affiliation: Department of Zoology,Guru Nanak Dev University,Amritsar (Punjab),143005,India.
authors.forename: A
authors.initials: A
authors.lastname: Thakur
authors.affiliation: Department of Microbiology,Guru Nanak Dev University,Amritsar (Punjab),143005,India.
authors.forename: P
authors.initials: P
authors.lastname: Dhammi
authors.affiliation: Department of Microbiology,Guru Nanak Dev University,Amritsar (Punjab),143005,India.
authors.forename: H S
authors.initials: HS
authors.lastname: Saini
authors.affiliation: Department of Zoology,Guru Nanak Dev University,Amritsar (Punjab),143005,India.
authors.forename: S
authors.initials: S
authors.lastname: Kaur
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D008136:Longevity / Q000187:drug effects; D018411:Spodoptera / Q000187:drug effects* / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D013307:Streptomycin
keywords: Spodoptera litura; antibiotic; enzyme activity; fitness of insect; gut microflora