title: Specific immunotherapy generates CD8(+) CD196(+) T cells to suppress lung cancer growth in mice.
abstract: That specific immunotherapy can inhibit cancer growth has been recognized; its efficiency is to be improved. This study aimed to inhibit lung cancer (LC) growth in a mouse model by using an LC-specific vaccination. In this study, a LC mouse model was created by adoptive transplantation with LC cells. The tumor-bearing mice were vaccinated with LC cell extracts plus adjuvant TNBS or adoptive transplantation with specific CD8(+) CD196(+) T cells. The results showed that the vaccination with LC extracts (LCE)/TNBS markedly inhibited the LC growth and induced CD8(+) CD196(+) T cells in LC tissue and the spleen. These CD8(+) CD196(+) T cells proliferated and produce high levels of perforin upon exposure to LCE and specifically induced LC cell apoptosis. Exposure to TNBS induced RAW264.7 cells to produce macrophage inflammatory protein-3α; the latter activated signal transducer and activator of transcription 3 and further induced perforin expression in the CD8(+) CD196(+) T cells. Adoptive transfer with specific CD8(+) CD196(+) T cells suppressed LC growth in mice. In conclusion, immunization with LC extracts and TNBS can induce LC-specific CD8(+) CD196(+) T cells in LC-bearing mice and inhibit LC growth.
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Infectious Disease Department, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Huiguo
authors.initials: H
authors.lastname: Chen
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Wu
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Li
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Liao
authors.affiliation: Thoracic Surgery Department, The Third Affiliated Hospital of Sun Yat-sen University, No. 600, Tianhe Road, Tianhe District, Guangzhou, 510630, China. lijiargu@163.com.
authors.forename: Lijia
authors.initials: L
authors.lastname: Gu
mesh_terms: D000818:Animals; D017209:Apoptosis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005260:Female; D016219:Immunotherapy, Adoptive / Q000379:methods*; D008175:Lung Neoplasms / Q000276:immunology / Q000628:therapy*; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D054353:Perforin / Q000378:metabolism; D000067996:RAW 264.7 Cells; D054399:Receptors, CCR6 / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism
chemical_list: D019496:Cancer Vaccines; D019402:Macrophage Inflammatory Proteins; D054399:Receptors, CCR6; D050796:STAT3 Transcription Factor; D054353:Perforin
keywords: LC; Mouse model; Specific immunotherapy; T helper cell; Vaccination