title: In Vitro Cytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials.
abstract: The aim of this study was to evaluate the cytotoxicity of three types of calcium silicate-based endodontic cement after different incubation periods with human periodontal ligament fibroblasts. Human periodontal ligament fibroblasts were cultured from extracted third molars and seeded in 96-well plates. MTA, calcium enriched mixture (CEM) cement, and Biodentine were prepared and added to culture insert plates which were immediately placed into 96-well plates containing cultured cells. After incubation periods of 24, 48, and 72 hours, cell viability was determined with WST-1 assay. Data were analysed statistically by ANOVA with repeated measures and Bonferroni tests. There was no significant difference in cell viability amongst the test materials after each incubation period (P > 0.05). MTA and CEM presented more than 90% cell viability after 24 and 48 hours of incubation and showed statistically significant decrease in cell viability after 72 hours of incubation (P < 0.05). Biodentine showed significantly less cell viability (73%) after 24 hours of incubation, whereas more than 90% cell viability was seen after 48 and 72 hours of incubation (P < 0.05). Despite the significant changes in cell viability over time, materials presented similar cytotoxicity profile. Biodentine and CEM can be considered as alternative materials for root-end surgery procedures.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: Selen
authors.initials: S
authors.lastname: Küçükkaya
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, 06100 Ankara, Turkey; Department of Endodontics, Dental College, University of Sharjah, Sharjah, UAE.
authors.forename: Mehmet Ömer
authors.initials: MÖ
authors.lastname: Görduysus
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: Naciye Dilara
authors.initials: ND
authors.lastname: Zeybek
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, 06100 Ankara, Turkey.
authors.forename: Sevda Fatma
authors.initials: SF
authors.lastname: Müftüoğlu