title: [PFKFB3 regulates 11'-deoxyverticillin A (C42)-induced autophagy and apoptosis in HeLa cells].
abstract: OBJECTIVE
  The aim is to reveal the role of PFKFB3 in 11'-deoxyverticillin A (C42)-induced autophagy and apoptosis.
  
  
  METHODS
  Electron and fluorescence microscopy, immunoblotting, MTS assay, siRNA interference and real time PCR were used.
  
  
  RESULTS
  C42 could induce multiple cell death in HeLa cells. Knockdown of either Beclin 1 or LC3, two important autophagic genes, increased both PARP-1 cleavage and cell viability loss. Although high dose of C42 triggered more cell viability loss, yet, it failed to augment autophagic flux. While PFKFB3 inhibitors attenuated C42-induced autophagy, the overexpression of PFKFB3 increased the induced autophagic flux.
  
  
  CONCLUSIONS
  PFKFB3 is involved in C42 induced-autophagy, which blunts the caspase-dependent apoptotic process.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Qin
authors.forename: Tongyang
authors.initials: T
authors.lastname: Chen
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Lu
authors.forename: Fengqing
authors.initials: F
authors.lastname: Hu
authors.forename: Huaiyi
authors.initials: H
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D004220:Disulfides / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D025481:Phosphofructokinase-2 / Q000235:genetics / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology*
chemical_list: D004220:Disulfides; D010879:Piperazines; C107345:PFKFB3 protein, human; D025481:Phosphofructokinase-2; C540279:11,11'-dideoxyverticillin A