title: 3D Printed Silicone-Hydrogel Scaffold with Enhanced Physicochemical Properties.
abstract: Scaffolds with multiple functionalities have attracted widespread attention in the field of tissue engineering due to their ability to control cell behavior through various cues, including mechanical, chemical, and electrical. Fabrication of such scaffolds from clinically approved materials is currently a huge challenge. The goal of this work was to fabricate a tissue engineering scaffold from clinically approved materials with the capability of delivering biomolecules and direct cell fate. We have used a simple 3D printing approach, that combines polymer casting with supercritical fluid technology to produce 3D interpenetrating polymer network (IPN) scaffold of silicone-poly(2-hydroxyethyl methacrylate)-co-poly(ethylene glycol) methyl ether acrylate (pHEMA-co-PEGMEA). The pHEMA-co-PEGMEA IPN materials were employed to support growth of human mesenchymal stem cells (hMSC), resulting in high cell viability and metabolic activity over a 3 weeks period. In addition, the IPN scaffolds support 3D tissue formation inside the porous scaffold with well spread cell morphology on the surface of the scaffold. As a proof of concept, sustained doxycycline (DOX) release from pHEMA-co-PEGMEA IPN was demonstrated and the biological activity of released drug from IPN was confirmed using a DOX regulated green fluorescent reporter (GFP) gene expression assay with HeLa cells. Given its unique mechanical and drug releasing characteristics, IPN scaffolds may be used for directing stem cell differentiation by releasing various chemicals from its hydrogel network.
authors.affiliation: DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark , Ørsteds Plads, DK-2800 Kgs, Lyngby, Denmark.
authors.forename: Soumyaranjan
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: BioModics ApS, Gregersensvej 7, DK-2630 Taastrup, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Alm
authors.affiliation: DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark , Ørsteds Plads, DK-2800 Kgs, Lyngby, Denmark.
authors.forename: Mette
authors.initials: M
authors.lastname: Hemmingsen
authors.affiliation: DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark , Ørsteds Plads, DK-2800 Kgs, Lyngby, Denmark.|Technical University of Denmark, DTU Nanotech, Center for Nanomedicine and Theranostics , 2800 Kgs, Denmark.
authors.forename: Alireza
authors.initials: A
authors.lastname: Dolatshahi-Pirouz
authors.affiliation: Danish Polymer Centre , Department of Chemical and Biochemical Engineering, Søltofts Plads, Building 229, DK-2800, Kgs, Lyngby, Denmark.
authors.forename: Jon
authors.initials: J
authors.lastname: Trifol
authors.affiliation: BioModics ApS, Gregersensvej 7, DK-2630 Taastrup, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Thomsen
authors.affiliation: DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark , Ørsteds Plads, DK-2800 Kgs, Lyngby, Denmark.
authors.forename: Martin
authors.initials: M
authors.lastname: Dufva
authors.affiliation: DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark , Ørsteds Plads, DK-2800 Kgs, Lyngby, Denmark.
authors.forename: Anders
authors.initials: A
authors.lastname: Wolff
authors.affiliation: DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark , Ørsteds Plads, DK-2800 Kgs, Lyngby, Denmark.
authors.forename: Jenny
authors.initials: J
authors.lastname: Emnéus
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004318:Doxycycline / Q000737:chemistry; D065546:Drug Liberation; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008689:Methacrylates / Q000737:chemistry; D066330:Printing, Three-Dimensional; D012828:Silicones / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D008689:Methacrylates; D012828:Silicones; C005044:hydroxyethyl methacrylate; D004318:Doxycycline