title: Microfluidic Breadboard Approach to Capillary Electrophoresis.
abstract: A breadboard approach for electrophoretic separations with contactless conductivity detection is presented. This is based on miniature off-the-shelf components such as syringe pumps, valves, and pressure controllers which could be set up in a very compact overall arrangement. It has a high flexibility for different tasks at hand, and the common operations of hydrodynamic injection and capillary flushing are automated. For demonstration of the versatility of the proposition, several very diverse configurations and modes of electrophoresis were successfully implemented, namely, standard capillary zone electrophoresis, pressure assisted zone electrophoresis, the simultaneous separation of cations and anions by dual-capillary zone electrophoresis, the separation of cationic amino acids by isotachophoresis, as well as the separation of small carboxylic acids by gradient elution moving boundary electrophoresis. The system also allows fast separations, as demonstrated by the analysis of six inorganic cations within 35 s. The approach addresses respective limitations of either conventional capillary electrophoresis instruments as well as electrophoretic lab-on-chip devices, while maintaining a performance in terms of detection limits and reproducibility comparable to standard instrumentation.
authors.affiliation: Department of Chemistry, University of Basel , Spitalstrasse 51, 4056 Basel, Switzerland.
authors.forename: Israel Joel
authors.initials: IJ
authors.lastname: Koenka
authors.affiliation: Department of Chemistry, University of Basel , Spitalstrasse 51, 4056 Basel, Switzerland.|Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá , Ctra. Madrid-Barcelona Km 33.6, Alcalá de Henares, 28871, Madrid, Spain.|University Institute of Research in Police Sciences (IUICP), University of Alcalá , Ctra. Madrid-Barcelona Km 33.6, 28871 Alcalá de Henares, Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Sáiz
authors.affiliation: Department of Chemistry, University of Basel , Spitalstrasse 51, 4056 Basel, Switzerland.
authors.forename: Paul
authors.initials: P
authors.lastname: Rempel
authors.affiliation: Department of Chemistry, University of Basel , Spitalstrasse 51, 4056 Basel, Switzerland.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Hauser