title: Altered heart proteome in fructose-fed Fisher 344 rats exposed to bisphenol A.
abstract: Bisphenol A (BPA), is an artificial estrogen initially produced for medical purposes but is today widely used in polycarbonate plastics and epoxy resins. Exposure-related reproductive disorders have been found, but recently it has also been suggested that BPA may be involved in obesity, diabetes, myocardial hypertrophy and myocardial infarction in humans. To mimic a modern lifestyle, female rats were fed with fructose or fructose plus BPA (0.25mg/L drinking water). The myocardial left ventricle proteome of water controls, fructose-fed and fructose-fed plus BPA supplemented rats was explored. The proteome was investigated using nano-liquid chromatography tandem mass spectrometry and two-dimensional gel electrophoresis followed by matrix assisted laser desorption/ionization mass spectrometry identification. In total, 41 proteins were significantly altered by BPA exposure compared to water or fructose controls. Principal component analysis and cellular process enrichment analysis of altered proteins suggested increased fatty acid transport and oxidation, increased ROS generation and altered structural integrity of the myocardial left ventricle in the fructose-fed BPA-exposed rats, indicating unfavorable effects on the myocardium. In conclusion, BPA exposure in the rats induces major alterations in the myocardial proteome.
authors.affiliation: Occupational and Environmental Medicine Center, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden. Electronic address: Stefan.ljunggren@liu.se.
authors.forename: S A
authors.initials: SA
authors.lastname: Ljunggren
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden. Electronic address: Madeleine.iggland@liu.se.
authors.forename: M
authors.initials: M
authors.lastname: Iggland
authors.affiliation: Department of Medical Sciences, Occupational and Environmental Medicine, Uppsala University, Uppsala, Sweden. Electronic address: Monika.ronn@medsci.uu.se.
authors.forename: M
authors.initials: M
authors.lastname: Rönn
authors.affiliation: Department of Medical Sciences, Cardiovascular Epidemiology, Uppsala University, Uppsala, Sweden. Electronic address: Lars.lind@medsci.uu.se.
authors.forename: L
authors.initials: L
authors.lastname: Lind
authors.affiliation: Department of Medical Sciences, Occupational and Environmental Medicine, Uppsala University, Uppsala, Sweden. Electronic address: Monica.lind@medsci.uu.se.
authors.forename: P M
authors.initials: PM
authors.lastname: Lind
authors.affiliation: Occupational and Environmental Medicine Center, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden. Electronic address: helen.m.karlsson@liu.se.
authors.forename: H
authors.initials: H
authors.lastname: Karlsson
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D005632:Fructose / Q000008:administration & dosage / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D006352:Heart Ventricles / Q000187:drug effects* / Q000378:metabolism*; D010636:Phenols / Q000633:toxicity*; D020543:Proteome / Q000096:biosynthesis*; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D005632:Fructose; D010636:Phenols; D020543:Proteome; D017382:Reactive Oxygen Species; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Endocrine disrupting chemicals; Fructose; Heart tissue; Proteomics