title: Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses.
abstract: The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins (S) were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected Vero- CCL-81 (monkey kidney), Huh-7 (human liver), and PK-15 (pig kidney) cells efficiently. CCL94 (cat kidney) cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Wanyu
authors.initials: W
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Anjun
authors.initials: A
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.|The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China. penggq@mail.hzau.edu.cn.|College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China. penggq@mail.hzau.edu.cn.|The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, 430070, China. penggq@mail.hzau.edu.cn.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Peng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents; D002460:Cell Line; D017934:Coronavirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D004353:Drug Evaluation, Preclinical; D000882:Haplorhini; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D008562:Membrane Glycoproteins; D011991:Receptors, Virus / Q000378:metabolism; D016415:Sequence Alignment; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D005760:Transmissible gastroenteritis virus; D056189:Viral Tropism; D053586:Virus Internalization
chemical_list: D000998:Antiviral Agents; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D064370:Spike Glycoprotein, Coronavirus
keywords: Coronavirus; cell entry; pseudotyped virus; receptor binding; spike proteins