title: Analysis of the Stromal Cellular Components of the Solid Tumor Microenvironment Using Flow Cytometry.
abstract: The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytometry. These protocols can be used for evaluating the cellular component of solid tumors from human or mouse origin or Matrigel plugs implanted in mice. The protocols describe the dissociation of tumor tissue with or without dissociation automatic devices. Subsequently, the use of flow cytometry for immunophenotypic analysis of host cells found in the tumor microenvironment, including myeloid derived suppressor cells, endothelial cells, and macrophages is provided. These methods can be used to broaden our understanding of the cross-talk between tumor and host cells in the tumor microenvironment. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Department of Cell Biology and Cancer Science, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Michael
authors.initials: M
authors.lastname: Timaner
authors.affiliation: Department of Cell Biology and Cancer Science, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Ofrat
authors.initials: O
authors.lastname: Beyar-Katz
authors.affiliation: Department of Cell Biology and Cancer Science, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Yuval
authors.initials: Y
authors.lastname: Shaked
mesh_terms: D000818:Animals; D001940:Breast / Q000166:cytology* / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology*; D002469:Cell Separation / Q000379:methods; D003094:Collagen / Q000737:chemistry; D003106:Colon / Q000166:cytology* / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D004338:Drug Combinations; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007797:Laminin / Q000737:chemistry; D051379:Mice; D011509:Proteoglycans / Q000737:chemistry; D017154:Stromal Cells / Q000473:pathology; D059016:Tumor Microenvironment
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen
keywords: bone marrow derived cells; cancer cells; cell dissociation; host