title: Polypeptide Functional Surface for the Aptamer Immobilization: Electrochemical Cocaine Biosensing.
abstract: Electroanalytical technologies as a beneficial subject of modern analytical chemistry can play an important role for abused drug analysis which is crucial for both legal and social respects. This article reports a novel aptamer-based biosensing procedure for cocaine analysis by combining the advantages of aptamers as selective recognition elements with the well-known advantages of biosensor systems such as the possibility of miniaturization and automation, easy fabrication and modification, low cost, and sensitivity. In order to construct the aptasensor platform, first, polythiophene bearing polyalanine homopeptide side chains (PT-Pala) was electrochemically coated onto the surface of an electrode and then cocaine aptamer was attached to the polymer via covalent conjugation chemistry. The stepwise modification of the surface was confirmed by electrochemical characterization. The designed biosensing system was applied for the detection of cocaine and its metabolite, benzoylecgonine (BE), which exhibited a linear correlation in the range from 2.5 up to 10 nM and 0.5 up to 50 μM for cocaine and BE, respectively. In order to expand its practical application, the proposed method was successfully tested for the analysis of synthetic biological fluids.
authors.forename: Guliz
authors.initials: G
authors.lastname: Bozokalfa
authors.affiliation: Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University , 34469 Istanbul, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Akbulut
authors.forename: Bilal
authors.initials: B
authors.lastname: Demir
authors.forename: Emine
authors.initials: E
authors.lastname: Guler
authors.forename: Z Pınar
authors.initials: ZP
authors.lastname: Gumus
authors.forename: Dilek
authors.initials: D
authors.lastname: Odaci Demirkol
authors.forename: Ebru
authors.initials: E
authors.lastname: Aldemir
authors.affiliation: Molecular Engineering Institute, Kinki University , 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Molecular Engineering Institute, Kinki University , 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Endo
authors.forename: Hakan
authors.initials: H
authors.lastname: Coskunol
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
authors.affiliation: Department of Chemistry, Faculty of Science and Letters, Istanbul Technical University , 34469 Istanbul, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Yagci
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D003042:Cocaine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D052157:Aptamers, Nucleotide; D010455:Peptides; C005618:benzoylecgonine; D003042:Cocaine