title: miR-19b attenuates H2O2-induced apoptosis in rat H9C2 cardiomyocytes via targeting PTEN.
abstract: Myocardial ischemia-reperfusion (I-R) injury lacks effective treatments. The miR-17-92 cluster plays important roles in regulating proliferation, apoptosis, cell cycle and other pivotal processes. However, their roles in myocardial I-R injury are largely unknown. In this study, we found that miR-19b was the only member of the miR-17-92 cluster that was downregulated in infarct area of heart samples from a murine model of I-R injury. Meanwhile, downregulation of miR-19b was also detected in H2O2-treated H9C2 cells in vitro mimicking oxidative stress occurring during myocardial I-R injury. Using flow cytometry and Western blot analysis, we found that overexpression of miR-19b decreased H2O2-induced apoptosis and improved cell survival, while downregulation of that had inverse effects. Furthermore, PTEN was negatively regulated by miR-19b at the protein level while silencing PTEN could completely block the aggravated impact of miR-19b inhibitor on H2O2-induced apoptosis in H9C2 cardiomyocytes, indicating PTEN as a downstream target of miR-19b controlling H2O2-induced apoptosis. These data indicate that miR-19b overexpression might be a novel therapy for myocardial I-R injury.
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Regeneration and Ageing Laboratory, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Bei
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital of NanTong University, Nantong, China.
authors.forename: Shengguang
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Che
authors.affiliation: Department of Cardiology, Shanghai Yangpu District Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Shanghai Yangpu District Hospital, Tongji University School of Medicine, Shanghai, China.
authors.forename: Hongbao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Regeneration and Ageing Laboratory, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.
authors.forename: Dongchao
authors.initials: D
authors.lastname: Lv
authors.affiliation: Regeneration and Ageing Laboratory, Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015536:Down-Regulation; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000235:genetics / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction
chemical_list: C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D006861:Hydrogen Peroxide; D051059:PTEN Phosphohydrolase
keywords: PTEN; Pathology Section; apoptosis; cardiomyocytes; ischemia reperfusion injury; microRNA