title: Lineage analysis by microsatellite loci deep sequencing in mice.
abstract: Lineage analysis is the identification of all the progeny of a single progenitor cell, and has become particularly useful for studying developmental processes and cancer biology. Here, we propose a novel and effective method for lineage analysis that combines sequence capture and next-generation sequencing technology. Genome-wide mononucleotide and dinucleotide microsatellite loci in eight samples from two mice were identified and used to construct phylogenetic trees based on somatic indel mutations at these loci, which were unique enough to distinguish and parse samples from different mice into different groups along the lineage tree. For example, biopsies from the liver and stomach, which originate from the endoderm, were located in the same clade, while samples in kidney, which originate from the mesoderm, were located in another clade. Yet, tissue with a common developmental origin may still contain cells of a mixed ancestry. This genome-wide approach thus provides a non-invasive lineage analysis method based on mutations that accumulate in the genomes of opaque multicellular organism somatic cells. Mol. Reprod. Dev. 83: 387-391, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: State Key Laboratory of Biocontrol, College of Ecology and Evolution, School of Life Sciences, Sun Yatsen University, Guangzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Biocontrol, College of Ecology and Evolution, School of Life Sciences, Sun Yatsen University, Guangzhou, China.|Collaborative Innovation Center of High Performance Computing, National University of Defense Technology, Changsha, China.
authors.forename: Xionglei
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory of Biocontrol, College of Ecology and Evolution, School of Life Sciences, Sun Yatsen University, Guangzhou, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xing
mesh_terms: D000818:Animals; D005260:Female; D056426:Genetic Loci; D055106:Genome-Wide Association Study; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D051379:Mice; D018895:Microsatellite Repeats