title: Recognition of a multiple antigen peptide containing sequence from mimotope of the dengue type 3 virus NS4B protein by human antibodies.
abstract: OBJECTIVE
  To evaluate the recognition of NS4B mimotope, as multiple antigen peptide (MAP), by dengue antibodies presents in serum samples from patients with different serotype infections.
  
  
  METHODS
  A MAP containing mimotope sequence was synthesized and used to evaluate the recognition of NS4B mimotope as MAP by a panel of 66 human sera from dengue cases by an indirect ELISA assay.
  
  
  RESULTS
  The MAP differentiated between sera from dengue viruses infected patients and sera from healthy individuals and the best reactivity was shown by serum from dengue type 3 virus patients. The recognition was more intense with serum from patients with secondary infection.
  
  
  CONCLUSIONS
  The findings suggest the potential use of NS4B mimotope on the development of a multi-epitope diagnostic tool. These results are important for further immunogenicity studies.
authors.affiliation: Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute, 11600, Havana, Cuba. Electronic address: namin@finlay.edu.cu.
authors.forename: Nevis
authors.initials: N
authors.lastname: Amin
authors.affiliation: 'Pedro Kourí' Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, 17100, Havana, Cuba.
authors.forename: Maritza
authors.initials: M
authors.lastname: Pupo
authors.affiliation: Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute, 11600, Havana, Cuba.
authors.forename: Alicia
authors.initials: A
authors.lastname: Aguilar
authors.affiliation: 'Pedro Kourí' Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, 17100, Havana, Cuba.
authors.forename: Susana
authors.initials: S
authors.lastname: Vázquez
authors.affiliation: 'Pedro Kourí' Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, 17100, Havana, Cuba.
authors.forename: Yamira
authors.initials: Y
authors.lastname: Caballero
authors.affiliation: Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute, 11600, Havana, Cuba.
authors.forename: Rolando
authors.initials: R
authors.lastname: Ochoa
authors.affiliation: 'Pedro Kourí' Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, 17100, Havana, Cuba.
authors.forename: María G
authors.initials: MG
authors.lastname: Guzmán
authors.affiliation: Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute, 11600, Havana, Cuba.
authors.forename: Armando
authors.initials: A
authors.lastname: Acosta
keywords: Antibodies; Dengue virus; MAP; NS4B