title: Transcriptome of larvae representing the Rhipicephalus sanguineus complex.
abstract: Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae) is possibly the most widespread tick species worldwide, responsible for transmitting several vector-borne pathogens of medical and veterinary importance. Here, we explore the transcriptome of R. sanguineus s.l. larvae (Putignano strain). We sequenced total RNA from R. sanguineus s.l. larvae. A total of 15,566,986 short paired-end reads were de novo-assembled into 33,396 transcripts and then annotated and analyzed. Particular attention was paid to transcripts putatively encoding ATP-binding proteins, due to their importance as mechanisms of detoxification and acaricide resistance. Additionally, microsatellite loci were investigated, as these are useful markers for population genetic studies. The present data and analyses provide a comprehensive transcriptomic resource for R. sanguineus. The results presented here will aid further genetic and genomic studies of this important tick species.
authors.affiliation: Department of Biology and Biotechnology, University of Pavia, Pavia, Italy; School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy.
authors.forename: Leone
authors.initials: L
authors.lastname: De Marco
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Epis
authors.affiliation: Department of Biology and Biotechnology, University of Pavia, Pavia, Italy; Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Comandatore
authors.affiliation: Department of Environmental Biology, University of Rome 'La Sapienza', Rome, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Porretta
authors.affiliation: Department of Veterinary Medicine, University 'Aldo Moro' of Bari, Bari, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cafarchia
authors.affiliation: Department of Environmental Biology, University of Rome 'La Sapienza', Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mastrantonio
authors.affiliation: Department of Veterinary Medicine, University 'Aldo Moro' of Bari, Bari, Italy; Department of Immunology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife, Brazil.
authors.forename: Filipe
authors.initials: F
authors.lastname: Dantas-Torres
authors.affiliation: Department of Veterinary Medicine, University 'Aldo Moro' of Bari, Bari, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Otranto
authors.affiliation: Department of Environmental Biology, University of Rome 'La Sapienza', Rome, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Urbanelli
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Bandi
authors.affiliation: Department of Biology and Biotechnology, University of Pavia, Pavia, Italy. Electronic address: davide.sassera@unipv.it.
authors.forename: Davide
authors.initials: D
authors.lastname: Sassera
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000818:Animals; D004285:Dogs; D063990:Gene Ontology; D056426:Genetic Loci; D007814:Larva / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D048508:Rhipicephalus sanguineus / Q000235:genetics*; D017422:Sequence Analysis, DNA; D059467:Transcriptome / Q000235:genetics*
chemical_list: D018528:ATP-Binding Cassette Transporters
keywords: ABC transporters; Larvae; Microsatellites; Rhipicephalus sanguineus; Transcriptome