title: Micro-photoluminescence of single living diatom cells.
abstract: Diatoms are single-celled microalgae that possess a nanostructured, porous biosilica shell called a frustule. This study characterized the micro-photoluminescence (μ-PL) emission of single living cells of the photosynthetic marine diatom Thalassiosira pseudonana in response to UV laser irradiation at 325 nm using a confocal Raman microscope. The photoluminescence (PL) spectrum had two primary peaks, one centered at 500-510 nm, which was attributed to the frustule biosilica, and a second peak at 680 nm, which was attributed to auto-fluorescence of photosynthetic pigments. The portion of the μ-PL emission spectrum associated with biosilica frustule in the single living diatom cell was similar to that from single biosilica frustules isolated from these diatom cells. The PL emission by the biosilica frustule in the living cell emerged only after cells were cultivated to silicon depletion. The discovery of the discovery of PL emission by the frustule biosilica within a single living diatom itself, not just its isolated frustule, opens up future possibilities for living biosensor applications, where the interaction of diatom cells with other molecules can be probed by μ-PL spectroscopy. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: LeDuff
authors.affiliation: Department of Microbiology, Oregon State University, Corvallis, OR, 97331, USA.
authors.forename: Guritno
authors.initials: G
authors.lastname: Roesijadi
authors.affiliation: School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, USA. rorrergl@engr.orst.edu.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Rorrer
mesh_terms: D015374:Biosensing Techniques; D017377:Diatoms / Q000737:chemistry* / Q000528:radiation effects; D008027:Light; D049449:Luminescence; D008855:Microscopy, Electron, Scanning; D014466:Ultraviolet Rays
keywords: biosilica; diatom; photoluminescence