title: Connectivity Measures in EEG Microstructural Sleep Elements.
abstract: During Non-Rapid Eye Movement sleep (NREM) the brain is relatively disconnected from the environment, while connectedness between brain areas is also decreased. Evidence indicates, that these dynamic connectivity changes are delivered by microstructural elements of sleep: short periods of environmental stimuli evaluation followed by sleep promoting procedures. The connectivity patterns of the latter, among other aspects of sleep microstructure, are still to be fully elucidated. We suggest here a methodology for the assessment and investigation of the connectivity patterns of EEG microstructural elements, such as sleep spindles. The methodology combines techniques in the preprocessing, estimation, error assessing and visualization of results levels in order to allow the detailed examination of the connectivity aspects (levels and directionality of information flow) over frequency and time with notable resolution, while dealing with the volume conduction and EEG reference assessment. The high temporal and frequency resolution of the methodology will allow the association between the microelements and the dynamically forming networks that characterize them, and consequently possibly reveal aspects of the EEG microstructure. The proposed methodology is initially tested on artificially generated signals for proof of concept and subsequently applied to real EEG recordings via a custom built MATLAB-based tool developed for such studies. Preliminary results from 843 fast sleep spindles recorded in whole night sleep of 5 healthy volunteers indicate a prevailing pattern of interactions between centroparietal and frontal regions. We demonstrate hereby, an opening to our knowledge attempt to estimate the scalp EEG connectivity that characterizes fast sleep spindles via an "EEG-element connectivity" methodology we propose. The application of the latter, via a computational tool we developed suggests it is able to investigate the connectivity patterns related to the occurrence of EEG microstructural elements. Network characterization of specified physiological or pathological EEG microstructural elements can potentially be of great importance in the understanding, identification, and prediction of health and disease.
authors.affiliation: Neurophysiology Unit, Department of Physiology, University of PatrasPatras, Greece; Department of Clinical Neurophysiology and Epilepsy, Guy's and St. Thomas' NHS Foundation TrustLondon, UK; Division of Neuroscience, Department of Basic and Clinical Neuroscience, King's College LondonLondon, UK.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Sakellariou
authors.affiliation: Neurophysiology Unit, Department of Physiology, University of Patras Patras, Greece.
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Koupparis
authors.affiliation: Neurophysiology Unit, Department of Physiology, University of Patras Patras, Greece.
authors.forename: Vasileios
authors.initials: V
authors.lastname: Kokkinos
authors.affiliation: Department of Clinical Neurophysiology and Epilepsy, Guy's and St. Thomas' NHS Foundation TrustLondon, UK; Division of Neuroscience, Department of Basic and Clinical Neuroscience, King's College LondonLondon, UK.
authors.forename: Michalis
authors.initials: M
authors.lastname: Koutroumanidis
authors.affiliation: Neurophysiology Unit, Department of Physiology, University of Patras Patras, Greece.
authors.forename: George K
authors.initials: GK
authors.lastname: Kostopoulos
keywords: EEG microstructure; EEG-element connectivity; imaginary part of coherence; sleep spindle connectivity; time-frequency analysis