title: Structural insights into the Aedes aegypti aquaporins and aquaglyceroporins - an in silico study.
abstract: There has been a fair bit of understanding on the structure-function relationship of Aquaporins (AQPs) from plants and vertebrates obtained from available X-ray crystallography data. However, there is a lacuna in understanding the structure of AQPs from sanguinivorous insects like the mosquito where it plays a crucial role in survival. In this study, we have built homology models for the Aedes aegypti AQPs, identified key channel lining residues and compared the structure and sequence with orthodox AQPs. Although Ar/R filter residues of AaAQP1 were exactly similar to orthodox AQPs, AaAQP2 has a substitution at LE1position possibly making it less efficient in high capacity water transport. The huge difference in the selectivity filter region of AaAQP3 suggests a different transport property for this channel. The changes observed in the H5 position of the filter of AaAQP4 and AaAQP5 may explain the presence of a larger pore aperture to permit the passage of larger solute molecules. AaAQP6 possesses a completely hydrophobic filter like that in mammalian super aquaporins. The identified key residues are pivotal in understanding the mechanism of action and gating of these channels.
authors.affiliation: a AU-KBC Research Centre, Madras Institute of Technology, Anna University , Chennai , India and.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Sreedharan
authors.affiliation: b Centre for Advanced Study in Crystallography and Biophysics, Guindy Campus, University of Madras , Chennai , India.
authors.forename: Gugan
authors.initials: G
authors.lastname: Kothandan
authors.affiliation: a AU-KBC Research Centre, Madras Institute of Technology, Anna University , Chennai , India and.
authors.forename: Kavitha
authors.initials: K
authors.lastname: Sankaranarayanan
mesh_terms: D000330:Aedes / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D051397:Aquaglyceroporins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020346:Aquaporins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003198:Computer Simulation; D013329:Structure-Activity Relationship
chemical_list: D051397:Aquaglyceroporins; D020346:Aquaporins
keywords: Homology modeling; ar/R selectivity filter; pore diameter