title: Immunological mechanisms involved in probiotic-mediated protection against Citrobacter rodentium-induced colitis.
abstract: Inflammatory bowel disease is a group of chronic, incurable inflammatory disorders of the gastrointestinal tract that cause severe diarrhoea, intestinal inflammation, pain, fatigue and weight loss. In this study, we first developed a model of Citrobacter rodentium-induced colitis and then evaluated the protective effects of selected probiotics on inflammation. The results showed that administration of a combination of probiotics including Lactobacillus rhamnosus ATCC 53103, Lactobacillus acidophilus ATCC 4356 and Lactobacillus plantarum A significantly increased the production of CD11c(+) dendritic cells in the spleen (3.62% vs phosphate buffered saline (PBS)-treated control, P<0.01) and mesenteric lymph nodes (MLNs). In addition, the presence of probiotics significantly up-regulated the development of CD4(+)/CD25(+)/Foxp3(+) regulatory T cells in MLNs by approximately 2.07% compared to the effect observed in the PBS-treated control (P<0.01) and down-regulated the expression of inflammatory cytokines, including interleukin-17, tumour necrosis factor-α and interferon-γ, by 0.11, 0.11 and 0.15%, respectively, compared to the effect observed in the PBS-treated control (P<0.01).These effects conferred protection against colitis, as shown by histopathological analyses.
authors.affiliation: 1 College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China P.R.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: 1 College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China P.R.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.affiliation: 2 Guangxi Veterinary Research Institute, 51 Aibei Road, Xixiangtang, Nanning, Guangxi, 530001, China P.R.
authors.forename: F
authors.initials: F
authors.lastname: Meng
authors.affiliation: 1 College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China P.R.
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.affiliation: 1 College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China P.R.
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.affiliation: 1 College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China P.R.
authors.forename: L
authors.initials: L
authors.lastname: Ye
authors.affiliation: 1 College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China P.R.
authors.forename: C
authors.initials: C
authors.lastname: Shi
authors.affiliation: 1 College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China P.R.
authors.forename: C
authors.initials: C
authors.lastname: Wang
mesh_terms: D044082:Citrobacter rodentium / Q000472:pathogenicity*; D003092:Colitis / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology; D007108:Immune Tolerance; D007155:Immunologic Factors / Q000008:administration & dosage*; D007778:Lactobacillus / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage*; D013154:Spleen / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D016207:Cytokines; D007155:Immunologic Factors
keywords: Citrobacter rodentium; colitis; dendritic cells; probiotics; regulatory T cells