title: [Effect of Dexamethasone on Blast Composition in Patients with Myelodysplastic Syndrome and Its Diagnostic Significance].
abstract: OBJECTIVE
  To analyze the effect of dexamethason (Dex) on blast composition in patients with myelodysplastic syndrome (MDS) and investigate its significance in diagnosis of MDS.
  
  
  METHODS
  The flow cytometry (FCM) was used to detect the blast rate and the expression of its antigens in 30 cases of MDS (10 cases were treated with Dex as DX group and 20 cases were treated without Dex as control group).
  
  
  RESULTS
  The difference of the CD34(+) cell number detected by FCM was not statistically significant between DX group and control group (P > 0.05); The rate of BM B cell precursors (BCP CD34(+)/CD19(+)/CD10(+) cells) increased in DX group significantly, and BM CD117(+) cells in CD34(+) cells was decreased significantly as compared with control group (P < 0.001). The expression of antigens between granulocyte and monocyte was not significantly different (P > 0.05).
  
  
  CONCLUSIONS
  The dexamethasone can increase the rate of BCP significantly and decreased the rate of BM CD117(+) cells in CD34(+) cells significantly. There is significant influence on the blast composition in MDS patients after dexamethasone treatment and without significant influence on the other phenotypcs.
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Key Laboratory of Clinical Laboratory Medicine of Henan Province, Faculty of Laboratorial Medicine of Zhengzhou University, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Key Laboratory of Clinical Laboratory Medicine of Henan Province, Faculty of Laboratorial Medicine of Zhengzhou University, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors.forename: Zhao-Bo
authors.initials: ZB
authors.lastname: Li
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Key Laboratory of Clinical Laboratory Medicine of Henan Province, Faculty of Laboratorial Medicine of Zhengzhou University, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Wang
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Key Laboratory of Clinical Laboratory Medicine of Henan Province, Faculty of Laboratorial Medicine of Zhengzhou University, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Laboratorial Medicine, The First Affiliated Hospital of Zhengzhou University, Key Laboratory of Clinical Laboratory Medicine of Henan Province, Faculty of Laboratorial Medicine of Zhengzhou University, Open Laboratory, Henan Province Key Subject of Clinical Medicine, Zhengzhou 450052, Henan Province, China. E-mail: ybh2002@163.com.
authors.forename: Bao-Hong
authors.initials: BH
authors.lastname: Yue
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D003907:Dexamethasone / Q000627:therapeutic use*; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D009000:Monocytes / Q000166:cytology; D009190:Myelodysplastic Syndromes / Q000188:drug therapy*; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D003907:Dexamethasone; D019009:Proto-Oncogene Proteins c-kit