title: Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site.
abstract: Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential.
authors.affiliation: Centre for Geobiology, University of Bergen, N-5020 Bergen, Norway ; Department of Biology, University of Bergen, N-5020 Bergen, Norway.
authors.forename: Juliane
authors.initials: J
authors.lastname: Wissuwa
authors.affiliation: Centre for Geobiology, University of Bergen, N-5020 Bergen, Norway ; Department of Biology, University of Bergen, N-5020 Bergen, Norway.
authors.forename: Runar
authors.initials: R
authors.lastname: Stokke
authors.affiliation: Centre for Geobiology, University of Bergen, N-5020 Bergen, Norway ; Department of Biology, University of Bergen, N-5020 Bergen, Norway.
authors.forename: Anita-Elin
authors.initials: AE
authors.lastname: Fedøy
authors.affiliation: The Norwegian Structural Biology Center (NorStruct), Department of Chemistry, UIT - The Arctic University of Norway, 9037 Tromsø, Norway.
authors.forename: Kjersti
authors.initials: K
authors.lastname: Lian
authors.affiliation: The Norwegian Structural Biology Center (NorStruct), Department of Chemistry, UIT - The Arctic University of Norway, 9037 Tromsø, Norway.
authors.forename: Arne Oskar
authors.initials: AO
authors.lastname: Smalås
authors.affiliation: Centre for Geobiology, University of Bergen, N-5020 Bergen, Norway ; Department of Biology, University of Bergen, N-5020 Bergen, Norway.
authors.forename: Ida Helene
authors.initials: IH
authors.lastname: Steen
keywords: Bioprospecting; Enzymes; Geobacillus; Thermophile