title: Mast cells positive to tryptase and tumour-associated macrophages correlate with angiogenesis in locally advanced colorectal cancer patients undergone to surgery.
abstract: OBJECTIVE
  The density of mast cells positive to tryptase (MCDPT) and tumor-associated macrophages (TAMs) were evaluated in a series of 87 patients with stage B and C colorectal cancer who had undergone radical surgery.
  
  
  METHODS
  MCDPT, TAMs, microvascular density (MVD), endothelial area (EA) and CD8(+) tumor infiltrating lymphocytes (CD8(+) TILs) were evaluated in tumor tissue samples by immunohistochemistry and image analysis. Each of the above parameters was correlated with the others and with the main clinico-pathological features.
  
  
  RESULTS
  A significant correlation between MCDPT, TAMs, MVD and EA was found by Pearson t-test analysis. With special references to the clinico-pathological features a minimal correlation using univariate analysis was found but it was not retained at multivariate analysis.
  
  
  CONCLUSIONS
  Our data suggest that MCDPT and TAMs are linked in the tumor microenvironment and play a role in CRC angiogenesis in a synergistic manner. The assessment of the combination MCDPT and TAMs could be evaluated as a target of novel anti-angiogenic therapies in colorectal cancer patients.
authors.affiliation: a Department of Medical and Surgery Science, Clinical Surgery Unit , University of Catanzaro 'Magna Graecia' Medical School , Catanzaro , Italy.|b Surgery Unit , National Cancer Research Centre, Giovanni Paolo II , Bari , Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Ammendola
authors.affiliation: c Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology , National Cancer Research Centre, 'Giovanni Paolo II' , Bari , Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Patruno
authors.affiliation: a Department of Medical and Surgery Science, Clinical Surgery Unit , University of Catanzaro 'Magna Graecia' Medical School , Catanzaro , Italy.
authors.forename: Rosario
authors.initials: R
authors.lastname: Sacco
authors.affiliation: c Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology , National Cancer Research Centre, 'Giovanni Paolo II' , Bari , Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Marech
authors.affiliation: a Department of Medical and Surgery Science, Clinical Surgery Unit , University of Catanzaro 'Magna Graecia' Medical School , Catanzaro , Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sammarco
authors.affiliation: d Health Science Department, Pathology Unit , University of Catanzaro 'Magna Graecia' Medical School , Catanzaro , Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Zuccalà
authors.affiliation: e Department of Medical and Surgery Science, Cardiovascular Disease Unit , University of Catanzaro 'Magna Graecia' Medical School , Catanzaro , Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Luposella
authors.affiliation: f Chair of Pathology, Veterinary Medical School , University of Bari , Bari , Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Zizzo
authors.affiliation: c Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology , National Cancer Research Centre, 'Giovanni Paolo II' , Bari , Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Gadaleta
authors.affiliation: c Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology , National Cancer Research Centre, 'Giovanni Paolo II' , Bari , Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Porcelli
authors.affiliation: c Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology , National Cancer Research Centre, 'Giovanni Paolo II' , Bari , Italy.
authors.forename: Cosmo Damiano
authors.initials: CD
authors.lastname: Gadaleta
authors.affiliation: g Department of Basic Medical Sciences, Neurosciences and Sensory Organs , University of Bari Medical School , Bari , Italy.|h National Cancer Institute 'Giovanni Paolo II' , Bari , Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.affiliation: c Diagnostic and Interventional Radiology Unit with Integrated Section of Translational Medical Oncology , National Cancer Research Centre, 'Giovanni Paolo II' , Bari , Italy.
authors.forename: Girolamo
authors.initials: G
authors.lastname: Ranieri
mesh_terms: D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D053802:Tryptases / Q000378:metabolism*
chemical_list: D053802:Tryptases
keywords: Angiogenesis; colorectal cancer; macrophages; mast cells; tryptase