title: Toxicopathic changes and genotoxic effects in liver of rat following exposure to diazinon.
abstract: In general, people may come in contact with mixtures of insecticides through domestic use, consumption of contaminated food or drinks, and/or living close to treated areas. We analyzed the toxic effects of diazinon on histological structure of liver and hematological parameters in male rats. DNA-damaging potential of diazinon was also investigated using the comet assay in blood cells and the micronucleus test in bone marrow. Two groups of six male rats orally received different amounts of diazinon: 1/50 and 1/25 LD 50 for 4 weeks (5 day/week). The present study showed that diazinon caused hypertrophy of sinusoids, central vein, and portal triad, in addition to the formation of oedema, vacuoles, hemorrhage, necrosis, and lymphoid infiltration in rats' liver. A significant decrease in red blood cells, hemoglobin, hematocrite levels, and platelet counts was observed in the treated groups. However, the white blood cell count increased. Micronucleus test results revealed aneugenic effects of diazinon. Furthermore, we noticed an increase in comet tail length in treated groups. So, the comet assay confirmed the genotoxic potential of diazinon in vivo. On the assumption that all alterations observed in rats could be observed in human, it is necessary to raise the awareness about the health risk posed by this insecticide.
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Lobna
authors.initials: L
authors.lastname: Ezzi
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Zohra
authors.initials: Z
authors.lastname: Haouas
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Imen Belhadj
authors.initials: IB
authors.lastname: Salah
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Amina
authors.initials: A
authors.lastname: Sakly
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Intissar
authors.initials: I
authors.lastname: Grissa
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Sana
authors.initials: S
authors.lastname: Chakroun
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Emna
authors.initials: E
authors.lastname: Kerkeni
authors.affiliation: Laboratory of Hematology, Fattouma Bourguiba University Hospital Monastir, Monastir, Tunisia.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Hassine
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Meriem
authors.initials: M
authors.lastname: Mehdi
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia. hassen.bencheikh@fmm.rnu.tn.
authors.forename: Hassen Ben
authors.initials: HB
authors.lastname: Cheikh
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects*; D020552:Comet Assay; D004249:DNA Damage; D003976:Diazinon / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D017208:Rats, Wistar; D059551:Toxicity Tests, Subacute
chemical_list: D004785:Environmental Pollutants; D007306:Insecticides; D003976:Diazinon
keywords: DNA damage; Diazinon; Hematological parameters; Hepatotoxicity