title: Gonadotropin and sf-1 regulation of cyp19a1a gene and aromatase activity during oocyte development in the rohu, L. rohita.
abstract: Cytochrome P450 aromatase (P450arom), a product of cyp19a1 gene, plays pivotal roles in vertebrate steroidogenesis and reproduction. In this study, we isolated partial cDNA encoding the ovarian (cyp19a1a) and brain (cyp19a1b) P450arom genes from adult female rohu, Labeo rohita and investigated the regulation of cyp19a1a by gonadotropin and SF-1. The cyp19a1a and cyp19a1b were expressed predominantly in the ovary and brain respectively, with quantity of the former attuned to reproductive cycle. To elucidate gonadotropin regulation of cyp19a1a mRNA expression and P450 aromatase activity for 17β-estradiol (E2) biosynthesis in vitro by the vitellogenic ovarian follicles, time- and dose-dependent studies were conducted with HCG and porcine FSH. Results demonstrated that HCG stimulated significantly higher expression of cyp19a1a mRNA and aromatase activity leading to increased biosynthesis of E2 than FSH. To understand the involvement of SF-1 to in the regulation of cyp19a1a and aromatase activity, ovarian follicles were incubated with increasing concentrations of HCG and expression of sf1gene and activation of SF-1 protein were measured. Results demonstrated that HCG significantly induced expression of sf-1 gene and activation of SF-1 protein suggesting a link between SF-1 and P450 aromatase activation in this fish ovary during gonadotropin-induced steroidogenesis.
authors.affiliation: Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India; Department of Zoology, Chandernagore College, Chandannagar, Hooghly, West Bengal 712136, India.
authors.forename: Sujata
authors.initials: S
authors.lastname: Roy Moulik
authors.affiliation: Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India; Department of Zoology, Taki Government College, Taki, Hasnabad, West Bengal 743429, India.
authors.forename: Puja
authors.initials: P
authors.lastname: Pal
authors.affiliation: Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India.
authors.forename: Suravi
authors.initials: S
authors.lastname: Majumder
authors.affiliation: Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India.
authors.forename: Buddhadev
authors.initials: B
authors.lastname: Mallick
authors.affiliation: Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India.
authors.forename: Shreyasi
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India.
authors.forename: Payel
authors.initials: P
authors.lastname: Guha
authors.affiliation: Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Jadavpur, Kolkata 700032, India.
authors.forename: Sibsankar
authors.initials: S
authors.lastname: Roy
authors.affiliation: Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address: dilipmukher@rediffmail.com.
authors.forename: Dilip
authors.initials: D
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000378:metabolism* / Q000494:pharmacology; D003530:Cyprinidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000502:physiology; D010802:Phylogeny; D054339:Steroidogenic Factor 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D029941:Fish Proteins; D054339:Steroidogenic Factor 1; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D001141:Aromatase
keywords: 17β-Estradiol; Brain; FSH; HCG; Labeo rohita; Ovary; P450 aromatase