title: Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts.
abstract: This study investigates the cardio-protective effect of Nos. 1 and 5 extracts from Taiwan Mingjian Oolong Tea on H9c2 cardiomyoblast cells treated with isoproterenol (ISO). Treatment with Nos. 1 and 5 extracts increased cell viability and blocked apoptosis in ISO exposed H9c2 cells. Moreover, Nos. 1 and 5 extracts blocked hypertrophy markers like G[Formula: see text]s, calcineurin, NFATc3, and BNP, thereby increasing cell proliferation markers -PI3K and AKT in a dose dependent manner. In contrast, apoptotic proteins, such as caspase-3 and cytochrome c were decreased in H9c2 cells treated with Nos. 1 and 5 extracts. We confirmed that the protective effect of No. 1 extract was partially mediated through the expression of ERK and p38, however, the No. 5 extract showed a protective effect via the ERK, JNK, and p38 pathways. This evidence provides new insights into the pharmacological role and therapeutic mechanism of Taiwan Mingjian Oolong Tea in heart diseases.
authors.affiliation: * Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan.|† Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Yeh
authors.affiliation: ‡ Graduate Institute of Basic Medical Science, Taichung, Taiwan.
authors.forename: Hsiang-I
authors.initials: HI
authors.lastname: Tsai
authors.affiliation: ¶ Department of Psychology, Asia University, Taichung, Taiwan.
authors.forename: Shiu-Min
authors.initials: SM
authors.lastname: Cheng
authors.affiliation: ** Division of Cardiology, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Peiying
authors.initials: P
authors.lastname: Pai
authors.affiliation: § School of Chinese Medicine, China Medical University, Taichung, Taiwan.|†† Chinese Medicine Department, China Medical University Beigang Hospital, Taiwan.
authors.forename: Tsung-Jung
authors.initials: TJ
authors.lastname: Ho
authors.affiliation: ‡‡ Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Ray-Jade
authors.initials: RJ
authors.lastname: Chen
authors.affiliation: §§ Division of Cardiology, Department of Internal Medicine, Armed Force Taichung General Hospital, Taichung, Taiwan.
authors.forename: Chao-Hung
authors.initials: CH
authors.lastname: Lai
authors.affiliation: ∥ Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Pei-Jane
authors.initials: PJ
authors.lastname: Huang
authors.affiliation: ¶¶ Department of Biotechnology, Bharathiar University, Coimbatore 641046, India.
authors.forename: V Vijaya
authors.initials: VV
authors.lastname: Padma
authors.affiliation: ‡ Graduate Institute of Basic Medical Science, Taichung, Taiwan.|∥ Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.|†† Chinese Medicine Department, China Medical University Beigang Hospital, Taiwan.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D028241:Camellia sinensis / Q000737:chemistry*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006984:Hypertrophy; D007545:Isoproterenol / Q000009:adverse effects*; D020935:MAP Kinase Signaling System; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013624:Taiwan; D013662:Tea / Q000737:chemistry*; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D002316:Cardiotonic Agents; D010936:Plant Extracts; D013662:Tea; D048051:p38 Mitogen-Activated Protein Kinases; D007545:Isoproterenol
keywords: Cardiac Apoptosis; Cardiac Hypertrophy; Isoproterenol; Oolong Tea