title: The relationship between transcript expression levels of nuclear encoded (TFAM, NRF1) and mitochondrial encoded (MT-CO1) genes in single human oocytes during oocyte maturation.
abstract: In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during oocyte maturation. The purpose of this study was to identify the relationship between transcript expression levels of the mitochondrial encoded gene (MT-CO1) and two nuclear encoded genes, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in various stages of human oocyte maturation. Nine consenting patients, age 21-35 years old, with male factors were selected for ovarian stimulation and intracytoplasmic sperm injection (ICSI) procedures. mRNA levels of mitochondrial-related genes were performed by singlecell TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant relationship between the relative expression levels in germinal vesicle (GV) stage oocytes (p = 0.62). On the contrary, a significant relationship was seen between the relative expression levels of TFAM and NRF1 and the MT-CO1 genes at the stages of metaphase I (MI) and MII (p = 0.03 and p = 0.002). A relationship exists between the transcript expression levels of TFAM and NRF1, and MT-CO1 genes in various stages of human oocyte maturation.
authors.affiliation: Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran.
authors.forename: M Ghaffari
authors.initials: MG
authors.lastname: Novin
authors.affiliation: Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Allahveisi
authors.affiliation: Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Noruzinia
authors.affiliation: Deptartment of Obstetrics and Gynecology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Farhadifar
authors.affiliation: Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: E
authors.initials: E
authors.lastname: Yousefian
authors.affiliation: Sarem Cell Research Center (SCRC), Sarem Women's Hospital, Tehran, Iran.
authors.forename: A Dehghani
authors.initials: AD
authors.lastname: Fard
authors.affiliation: Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Salimi
keywords: Mitochondria; Oocyte maturation; Quantitative real-time polymerase chain reaction (qRT-PCR); single-cell