title: Tumor-induced remote ECM network orientation steers angiogenesis.
abstract: Tumor angiogenesis promotes tumor growth and metastasis. Here, we use automated sequential microprinting of tumor and endothelial cells in extracellular matrix (ECM) scaffolds to study its mechanical aspects. Quantitative reflection microscopy shows that tumor spheroids induce radial orientation of the surrounding collagen fiber network up to a distance of five times their radius. Across a panel of ~20 different human tumor cell lines, remote collagen orientation is correlated with local tumor cell migration behavior. Tumor induced collagen orientation requires contractility but is remarkably resistant to depletion of collagen-binding integrins. Microvascular endothelial cells undergo directional migration towards tumor spheroids once they are within the tumor-oriented collagen fiber network. Laser ablation experiments indicate that an intact physical connection of the oriented network with the tumor spheroid is required for mechanical sensing by the endothelial cells. Together our findings indicate that, in conjunction with described activities of soluble angiogenic factors, remote physical manipulation of the ECM network by the tumor can help to steer angiogenesis.
authors.affiliation: Division of Toxicology, Leiden Academic Center for Drug Research, Leiden University, Leiden, the Netherlands.
authors.forename: Hayri E
authors.initials: HE
authors.lastname: Balcioglu
authors.affiliation: Division of Toxicology, Leiden Academic Center for Drug Research, Leiden University, Leiden, the Netherlands.
authors.forename: Bob
authors.initials: B
authors.lastname: van de Water
authors.affiliation: Division of Toxicology, Leiden Academic Center for Drug Research, Leiden University, Leiden, the Netherlands.
authors.forename: Erik H J
authors.initials: EH
authors.lastname: Danen
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005109:Extracellular Matrix; D006801:Humans; D051379:Mice; D009389:Neovascularization, Pathologic