title: Salicylic acid-induced changes in physiological parameters and genes of the flavonoid biosynthesis pathway in Artemisia vulgaris and Dendranthema nankingense during aphid feeding.
abstract: Phloem-feeding aphids cause serious damage to plants. The mechanisms of plant-aphid interactions are only partially understood and involve multiple pathways, including phytohormones. In order to investigate whether salicylic acid (SA) is involved and how it plays a part in the defense response to the aphid Macrosiphoniella sanbourni, physiological changes and gene expression profiles in response to aphid inoculation with or without SA pretreatment were compared between the aphid-resistant Artemisia vulgaris 'Variegata' and the susceptible chrysanthemum, Dendranthema nankingense. Changes in levels of reactive oxygen species, malondialdehyde (MDA), and flavonoids, and in the expression of genes involved in flavonoid biosynthesis, including PAL (phenylalanine ammonia-lyase), CHS (chalcone synthase), CHI (chalcone isomerase), F3H (flavanone 3-hydroxylase), F3'H (flavanone 3'-hydroxylase), and DFR (dihydroflavonol reductase), were investigated. Levels of hydrogen peroxide, superoxide anions, MDA, and flavonoids, and their related gene expression, increased after aphid infestation and SA pretreatment followed by aphid infestation; the aphid-resistant A. vulgaris exhibited a more rapid response than the aphid-susceptible D. nankingense to SA treatment and aphid infestation. Taken together, our results suggest that SA could be used to increase aphid resistance in the chrysanthemum.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Xia
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
authors.forename: F D
authors.initials: FD
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, China.
authors.forename: G S
authors.initials: GS
authors.lastname: Lv
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001042:Aphids / Q000472:pathogenicity / Q000502:physiology*; D019306:Artemisia / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000469:parasitology; D031202:Chrysanthemum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000469:parasitology; D005247:Feeding Behavior / Q000502:physiology; D005419:Flavonoids / Q000096:biosynthesis; D018506:Gene Expression Regulation, Plant; D006790:Host-Parasite Interactions; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D005419:Flavonoids; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D006899:Mixed Function Oxygenases; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase; C048797:flavanone 3-dioxygenase; D000217:Acyltransferases; C020141:flavanone synthetase; D010650:Phenylalanine Ammonia-Lyase; D019753:Intramolecular Lyases; C019865:chalcone isomerase; D020156:Salicylic Acid