title: Biological valorization of pure and crude glycerol into 1,3-propanediol using a novel isolate Lactobacillus brevis N1E9.3.3.
abstract: The aim of the study was to evaluate a novel onsite enrichment approach to isolate a crude glycerol utilizing facultative anaerobic bacteria. An onsite enrichment in natural conditions resulted an isolate, Lactobacillus brevis N1E9.3.3, that can utilize glycerol and produce 1,3-propanediol with a yield of 0.89g1,3-PDO/gGlycerol and productivity of 0.78g1,3-PDO/l/h at pH-8.5 under anaerobic conditions. Batch fermentation experiments with glycerol-glucose co-fermentation strategy was carried out to evaluate the production of 1,3-propanediol and other byproducts. The effect of other carbon sources as co-substrate was also evaluated. At the optimized condition, 18.6g/l 1,3-propanediol was monitored when biodiesel industry generated crude glycerol and 2.5% glucose were used as the substrate.
authors.affiliation: Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-NIIST, Thiruvananthapuram 695019, Kerala, India.
authors.forename: Narisetty
authors.initials: N
authors.lastname: Vivek
authors.affiliation: Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Biotechnology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India. Electronic address: binodkannur@niist.res.in.
authors.forename: Parameswaran
authors.initials: P
authors.lastname: Binod
mesh_terms: D061249:Batch Cell Culture Techniques; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D052196:Levilactobacillus brevis / Q000302:isolation & purification / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D011409:Propylene Glycols; C041787:1,3-propanediol; D005947:Glucose; D005990:Glycerol
keywords: 1,3-Propanediol; Bioconversion; Crude glycerol; Lactobacillus brevis; Self anaerobiosis