title: miR-100 Inhibits the Growth and Migration of Burn-Denatured Fibroblasts.
abstract: BACKGROUND Burn-denatured dermis is able to regain the function and shape of normal dermis; however, the potential mechanisms are still vague. The aim of this study was to investigate roles of miR-100 involved in the growth and migration of burn-denatured fibroblasts. MATERIAL AND METHODS Quantitative real-time polymerase chain reaction(qRT-PCR) was used to assess the expression of miR-100. Transient transfection of miR-100 mimics and inhibitor was used to up-regulate or down-regulate the expression of miR-100. Cell proliferation and colony formation assay were used to test the cell growth, and wound healing assay and transwell migration assay were used to evaluate the cell migration. RESULTS miR-100 expression was notably downregulated in the burn-denatured fibroblasts compared to normal controls. Functionally, transfection of miR-100 inhibitors improved the growth and migration abilities of burn-denatured fibroblasts. In contrast, upregulation of miR-100 inhibits the growth and migration of burn-denatured fibroblasts. CONCLUSIONS Based on these observations, we concluded that miR-100 can inhibit the growth and migration of burn-denatured fibroblasts.
authors.affiliation: Burn and Wound Healing Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Jianghui
authors.initials: J
authors.lastname: Ying
authors.affiliation: Burn and Wound Healing Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Burn and Wound Healing Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Ruijin
authors.initials: R
authors.lastname: Yang
authors.affiliation: Burn and Wound Healing Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Burn and Wound Healing Centre, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
authors.forename: Jianjun
authors.initials: J
authors.lastname: Xu
mesh_terms: D002056:Burns / Q000235:genetics* / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C544266:MIRN100 microRNA, human; D035683:MicroRNAs