title: Influence of Toothbrushing on the Antierosive Effect of Film-Forming Agents.
abstract: This study evaluated the influence of toothbrushing on the antierosive effect of solutions containing sodium fluoride (225 ppm/F), stannous chloride (800 ppm/Sn), sodium linear polyphosphate (2%/LPP), and their combinations, and deionized water as negative control (C). Solutions were tested in a 5-day erosion-remineralization-abrasion cycling model, using enamel and dentin specimens (n = 8). Erosion was performed 6 times/day for 5 min, exposure to the test solutions 3 times/day for 2min, and toothbrushing (or not) with toothpaste slurry 2 times/day for 2 min (45 strokes). Surface loss (SL) was determined by noncontact profilometry. Data were analyzed using three-way ANOVA (α = 0.05). Brushing caused more SL than no brushing for enamel (mean ± SD, in micrometers: 52.7 ± 6.6 and 33.0 ± 4.5, respectively), but not for dentin (28.2 ± 1.9 and 26.6 ± 1.8, respectively). For enamel without brushing, F+LPP+Sn showed the lowest SL (23.8 ± 3.4), followed by F+Sn (30.6 ± 4.9) and F+LPP (31.7 ± 1.7), which did not differ from each other. No differences were found between the other groups and C (37.8 ± 2.1). When brushing, F+LPP+Sn exhibited the lowest SL (36.7 ± 2.4), not differing from F+LPP (39.1 ± 1.8). F, F+Sn and LPP+Sn were similar (46.7 ± 2.9, 42.1 ± 2.8 and 45.3 ± 4.6, respectively) and better than C (52.7 ± 4.3). Sn (55.0 ± 2.4) and LPP (51.0 ± 4.3) did not differ from C. For dentin, neither groups differed from C, regardless of brushing. In conclusion, toothbrushing did not affect the antierosive effect of F+Sn, F+LPP and F+LPP+Sn on enamel, although overall it led to more erosion than nonbrushing. F and LPP+Sn showed a protective effect only under brushing conditions, whereas Sn and LPP did not exhibit any protection. For dentin, neither toothbrushing nor the test solutions influenced the development of erosion.
authors.affiliation: Department of Restorative Dentistry, University of Sx00E3;o Paulo School of Dentistry, Sx00E3;o Paulo, Brazil.
authors.forename: Taís
authors.initials: T
authors.lastname: Scaramucci
authors.forename: Samira Helena
authors.initials: SH
authors.lastname: João-Souza
authors.forename: Frank
authors.initials: F
authors.lastname: Lippert
authors.forename: George J
authors.initials: GJ
authors.lastname: Eckert
authors.forename: Idalina V
authors.initials: IV
authors.lastname: Aoki
authors.forename: Anderson T
authors.initials: AT
authors.lastname: Hara
mesh_terms: D000818:Animals; D002327:Cariostatic Agents / Q000494:pharmacology*; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D010710:Phosphates / Q000494:pharmacology*; D012463:Saliva / Q000502:physiology; D012969:Sodium Fluoride / Q000494:pharmacology*; D017971:Tin Compounds / Q000494:pharmacology*; D014072:Tooth Abrasion / Q000209:etiology / Q000517:prevention & control; D014077:Tooth Erosion / Q000209:etiology / Q000517:prevention & control*; D014089:Tooth Remineralization; D014099:Toothbrushing / Q000009:adverse effects*
chemical_list: D002327:Cariostatic Agents; D010710:Phosphates; D017971:Tin Compounds; C023599:stannous chloride; D012969:Sodium Fluoride; C018279:sodium phosphate