title: Potential biodegradation of crude petroleum oil by newly isolated halotolerant microbial strains from polluted Red Sea area.
abstract: Two microbial isolates from oil polluted Red Sea water in Egypt, designated as RS-Y1 and RS-F3, were found capable of degrading Belayim mix (BX) crude oil. Strains RS-Y1 and RS-F3 were assigned to the genera Lipomyces tetrasporus and Paecilomyces variotii based on their morphological and physiological characteristics. Both isolates were compared for the biodegradation of crude petroleum-oil hydrocarbons in basal salt medium supplemented with 5% (w/v) of BX-crude oil. Gas chromatography profile showed that the biodegradation of total petroleum hydrocarbons (TPHs) inoculated with L. tetrasporus (68.3%) and P. variotii (58.15%) along with their consortium (66%) significantly reduced TPHs levels as compared to the control after 30days. L. tetrasporus (44.5%) was more effective than P. variotii strain (32.89%) in reducing the unresolved complex mixtures (UCM) content from the medium. Both isolates exhibited a strong growth over a wide range of salinity (5-45g/L NaCl).
authors.affiliation: Microbiology Department, Faculty of Science, Ain shams University, Egypt.
authors.forename: Yousseria M H
authors.initials: YMH
authors.lastname: Shetaia
authors.affiliation: Egyptian Petroleum Research Institute (EPRI), Cairo, Egypt.
authors.forename: Wafaa A A
authors.initials: WAA
authors.lastname: El Khalik
authors.affiliation: Department of Chemistry, Faculty of Science, Tanta University, Tanta, Egypt.
authors.forename: Tarek M
authors.initials: TM
authors.lastname: Mohamed
authors.affiliation: Egyptian Petroleum Research Institute (EPRI), Cairo, Egypt.
authors.forename: Laila A
authors.initials: LA
authors.lastname: Farahat
authors.affiliation: Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City, Egypt; School of Chemical Engineering, University of Adelaide, Adelaide, Australia. Electronic address: ahmed.elmekawy@adelaide.edu.au.
authors.forename: Ahmed
authors.initials: A
authors.lastname: ElMekawy
mesh_terms: D001673:Biodegradation, Environmental; D004534:Egypt; D006838:Hydrocarbons / Q000378:metabolism; D055160:Lipomyces / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D010143:Paecilomyces / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution; D012623:Seawater / Q000382:microbiology; D014876:Water Pollution
chemical_list: D006838:Hydrocarbons; D010578:Petroleum
keywords: Biodegradation; Crude oil; Halotolerant; Lipomyces; Paecilomyces