title: Mechanical and Morphological Analysis of Cancer Cells on Nanostructured Substrates.
abstract: Cancer metastasis is a major cause of cancer-induced deaths in patients. Mimicking nanostructures of an extracellular matrix surrounding cancer cells can provide useful clues for metastasis. This paper compares the morphology, proliferation, spreading, and stiffness of highly aggressive glioblastoma multiforme cancer cells and normal fibroblast cells seeded on a variety of ordered polymeric nanostructures (nanopillars and nanochannels). Both cell lines survive and proliferate on the nanostructured surface and show more similarity on nanostructured surfaces than on flat surfaces. Although both show similar stiffness on the nanochannel surface, glioblastomas are softer, spread to a larger area, and elongate less than fibroblasts. The nanostructured surfaces are useful for in vitro model of an extracellular matrix to study the cancer cell migratory phenotype.
authors.affiliation: Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences , Wenzhou Medical University, Wenzhou, Zhejiang 325001, People's Republic of China.|Department of Chemical Engineering, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Dandan
authors.initials: D
authors.lastname: Ning
authors.forename: Binh
authors.initials: B
authors.lastname: Duong
authors.forename: Gawain
authors.initials: G
authors.lastname: Thomas
authors.forename: Yong
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Chemical Engineering, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Ma
authors.forename: Qi
authors.initials: Q
authors.lastname: Wen
authors.affiliation: Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences , Wenzhou Medical University, Wenzhou, Zhejiang 325001, People's Republic of China.|Department of Chemical Engineering, Northeastern University , Boston, Massachusetts 02115, United States.
authors.forename: Ming
authors.initials: M
authors.lastname: Su
mesh_terms: D000180:Acrylic Resins; D000199:Actins / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D004126:Dimethylformamide; D004996:Ethidium / Q000031:analogs & derivatives; D005109:Extracellular Matrix / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D007211:Indoles; D051379:Mice; D041681:NIH 3T3 Cells; D049329:Nanostructures
chemical_list: D000180:Acrylic Resins; D000199:Actins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007211:Indoles; C085925:calcein AM; C010504:polyacrylonitrile; C007293:DAPI; C018533:ethidium homodimer; D004126:Dimethylformamide; D004996:Ethidium; D016650:Fluorescein-5-isothiocyanate