title: Functional Nanoparticles Activate a Decellularized Liver Scaffold for Blood Detoxification.
abstract: Extracorporeal devices have great promise for cleansing the body of virulence factors that are caused by venomous injuries, bacterial infections, and biological weaponry. The clinically used extracorporeal devices, such as artificial liver-support systems that are mainly based on dialysis or electrostatic interaction, are limited to remove a target toxin. Here, a liver-mimetic device is shown that consists of decellularized liver scaffold (DLS) populated with polydiacetylene (PDA) nanoparticles. DLS has the gross shape and 3D architecture of a liver, and the PDA nanoparticles selectively capture and neutralize the pore-forming toxins (PFTs). This device can efficiently and target-orientedly remove PFTs in human blood ex vivo without changing blood components or activating complement factors, showing potential application in antidotal therapy. This work provides a proof-of-principle for blood detoxification by a nanoparticle-activated DLS, and can lead to the development of future medical devices for antidotal therapy.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.|State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Du
authors.affiliation: Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu, 610041, P. R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of NanoEngineering, University of California, San Diego, CA, 92093, USA.
authors.forename: Shaochen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Du
authors.affiliation: Laboratory of Pathology, West China Hospital, Sichuan University, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, 610041, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
authors.forename: Maling
authors.initials: M
authors.lastname: Gou
mesh_terms: D000818:Animals; D032701:Biomimetics; D006801:Humans; D008658:Inactivation, Metabolic; D008099:Liver / Q000166:cytology*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry; D053279:Polyynes / Q000737:chemistry; D051381:Rats; D012996:Solutions; D054457:Tissue Scaffolds / Q000737:chemistry*; D014118:Toxins, Biological / Q000302:isolation & purification
chemical_list: D000078789:Polyacetylene Polymer; D011108:Polymers; D012996:Solutions; D014118:Toxins, Biological; D053279:Polyynes; C082361:polydiacetylene
keywords: biomimetics; blood cleansing; decellularized scaffold; detoxification; nanoparticles