title: Green banana pasta diet prevents oxidative damage in liver and kidney and improves biochemical parameters in type 1 diabetic rats.
abstract: OBJECTIVE
  In this study, the effects of a green banana pasta diet on the oxidative damage from type 1 diabetes mellitus (DM) were investigated.
  
  
  METHODS
  Formulations containing 25 (F25), 50 (F50), and 75% (F75) of green banana pasta were prepared and included in a 12-week diet of Wistar rats with alloxan-induced type 1 DM. The effects of these formulations in preventing oxidative damage in kidneys and liver homogenates of rats were evaluated using the TBARS assay (lipid peroxidation in liver) and the DNPH assay (protein oxidation in liver and kidneys). Furthermore, the effects of the formulations on the fasting glycemia, fructosamine levels, renal function (creatinine), liver function (enzymes aspartate aminotransferase [AST] and alanine aminotransferase [ALT]), and lipid profile (total cholesterol and fractions) in the serum of rats were evaluated in addition to the evaluation of the centesimal composition and microbiological analysis of the produced green banana pasta.
  
  
  RESULTS
  An F75 diet prevented hyperglycemia in diabetic rats (p < 0.05) compared to the diabetic rats fed a standard diet (commercial feed). Notably, the protein oxidation in both the liver and kidneys were prevented in diabetic rats on the F50 or F75 diets compared to the control group, whereas the lipid peroxidation was only prevented in the liver (p < 0.05). Moreover, all formulations prevented an increase in the amount of triglycerides in the serum of the rats. The F25 and F50 diet prevented the increase of cholesterol, and the F75-based diet of ALT and fructosamine (p < 0.05) supported the anti-hyperglycemic effects and the protection against oxidative damage.
  
  
  CONCLUSIONS
  The green banana pasta (F75) diet showed great potential for preventing complications associated with diabetes.
authors.affiliation: Laboratório de Pesquisa em Ciências Biológicas, Universidade José do Rosário Vellano (Unifenas), Alfenas, MG, Brasil.
authors.forename: Aline Rodrigues da
authors.initials: AR
authors.lastname: Silva
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (Unifal), Alfenas, MG, Brasil.
authors.forename: Cláudio Daniel
authors.initials: CD
authors.lastname: Cerdeira
authors.affiliation: Laboratório de Pesquisa em Ciências Biológicas, Universidade José do Rosário Vellano (Unifenas), Alfenas, MG, Brasil.
authors.forename: Anelise Rigoni
authors.initials: AR
authors.lastname: Brito
authors.affiliation: Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas da Faculdade de Ciências Farmacêuticas, Unifal, Alfenas, MG, Brasil.
authors.forename: Bruno Cesar Correa
authors.initials: BC
authors.lastname: Salles
authors.affiliation: Laboratório de Pesquisa em Ciências Biológicas, Universidade José do Rosário Vellano (Unifenas), Alfenas, MG, Brasil.
authors.forename: Gabriela Franzin
authors.initials: GF
authors.lastname: Ravazi
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas (Unifal), Alfenas, MG, Brasil.
authors.forename: Gabriel de Oliveira Isac
authors.initials: Gde O
authors.lastname: Moraes
authors.affiliation: Laboratório de Pesquisa em Ciências Biológicas, Universidade José do Rosário Vellano (Unifenas), Alfenas, MG, Brasil.
authors.forename: Luciana Rosa Alves
authors.initials: LR
authors.lastname: Rufino
authors.affiliation: Laboratório de Biologia e Fisiologia de Micro-organismos, Unifenas, Alfenas, MG, Brasil.
authors.forename: Rafaela Bergmann Strada de
authors.initials: RB
authors.lastname: Oliveira
authors.affiliation: Laboratório de Pesquisa em Ciências Biológicas, Universidade José do Rosário Vellano (Unifenas), Alfenas, MG, Brasil.
authors.forename: Gérsika Bitencourt
authors.initials: GB
authors.lastname: Santos
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D003404:Creatinine / Q000097:blood; D048909:Diabetes Complications / Q000378:metabolism / Q000517:prevention & control; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000517:prevention & control; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism* / Q000517:prevention & control*; D004035:Diet Therapy / Q000379:methods*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D028521:Musa / Q000737:chemistry*; D018384:Oxidative Stress / Q000502:physiology*; D017208:Rats, Wistar; D012016:Reference Values; D015203:Reproducibility of Results; D017392:Thiobarbituric Acid Reactive Substances; D016896:Treatment Outcome; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D002784:Cholesterol; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase