title: Synthesis of octyl-β-D-glucopyranoside catalyzed by Thai rosewood β-glucosidase-displaying Pichia pastoris in an aqueous/organic two-phase system.
abstract: We explored the ability of a Thai rosewood β-glucosidase-displaying P. pastoris whole-cell biocatalyst (Pp-DCBGL) system to synthesize alkyl β-D-glucosides. The primary investigation centered on the synthesis of octyl-β-D-glucopyranoside (octyl-glu, OG). OG could be synthesized through reverse hydrolysis reaction with very low efficiency. Then, OG was synthesized between BG and octanol by a transglycosylation reaction. In a 2-ml reaction system, OG was synthesized with a conversion rate of 51.1% in 3h when 5 mg/ml BG was utilized as the glucosyl donor under optimized conditions. And, even after being reused four times, the Pp-DCBGL was relatively stable. Additionally, a 500-ml-scale reaction system was conducted in a 2-L stirred reactor with a conversion rate of 47.5% in 1.5 h. Moreover, the conversion rate did not decrease after the whole-cell catalyst was reused two times. In conclusion, Pp-DCBGL has high reaction efficiency and operational stability, which is a powerful biocatalyst available for industrial synthesis.
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: DongHeng
authors.initials: D
authors.lastname: Guo
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: YanShan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
authors.forename: YaJun
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. Electronic address: syhan@scut.edu.cn.
authors.forename: ShuangYan
authors.initials: S
authors.lastname: Han
authors.affiliation: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China. Electronic address: spzheng@scut.edu.cn.
authors.forename: SuiPing
authors.initials: S
authors.lastname: Zheng
mesh_terms: D055162:Biocatalysis; D019149:Bioreactors; D035782:Dalbergia / Q000201:enzymology / Q000235:genetics; D004795:Enzyme Stability; D005285:Fermentation; D005960:Glucosides / Q000096:biosynthesis*; D006031:Glycosylation; D006868:Hydrolysis; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013785:Thailand; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D005960:Glucosides; D010940:Plant Proteins; D011994:Recombinant Proteins; C018619:octyl-beta-D-glucoside; D001617:beta-Glucosidase
keywords: Octyl-β-d-glucopyranoside; Scale synthesis; Thai rosewood β-glucosidase; Transglycosylation; Yeast whole-cell catalyst