title: Experimental infection of SPF and Korean native chickens with highly pathogenic avian influenza virus (H5N8).
abstract: In 2014, an H5N8 outbreak of highly pathogenic avian influenza (HPAI) occurred in South Korea. The H5N8 strain produced mild to moderate clinical signs and mortality rates in commercial chicken farms, especially Korean native chicken farms. To understand the differences between their pathogenicity in SPF chicken and Korean native chicken., we evaluated the mean bird lethal doses (BLD50) of the Korean representative H5N8 virus (A/broiler duck/Korea/Buan2/2014) The BLD50values of the H5N8 virus were 10(5.3)EID50 and 10(6.7)EID50 in SPF and Korean native chickens, respectively. In addition, the mean death time was much longer, and the viral titers in tissues of H5N8-infected chickens were significantly lower, in the Korean group than in the SPF group. These features of the H5N8 virus likely account for its mild-to-moderate pathogenicity in commercial chicken farms, especially Korean native chicken flocks, despite the fact that it is a highly pathogenic virus according to the OIE criteria. To improve current understanding and management of HPAI, pathogenic characterization of novel emerging viruses should be performed by natural route in major poultry species in each country.
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Republic of Korea.
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Republic of Korea.
authors.forename: Byung-Min
authors.initials: BM
authors.lastname: Song
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Republic of Korea.
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Kang
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Republic of Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Woo
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Republic of Korea.
authors.forename: Gyeong-Beom
authors.initials: GB
authors.lastname: Heo
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Republic of Korea.
authors.forename: Suk Chan
authors.initials: SC
authors.lastname: Jung
authors.affiliation: College of Veterinary Medicine, Seoul National University, 599 Gwanakro, Seoul, 151-742, Republic of Korea.
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Park
authors.affiliation: Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Republic of Korea leeyj700@korea.kr kimhong@snu.ac.kr.
authors.forename: Youn-Jeong
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Seoul National University, 599 Gwanakro, Seoul, 151-742, Republic of Korea leeyj700@korea.kr kimhong@snu.ac.kr.
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D009980:Influenza A virus / Q000145:classification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000821:virology*; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
keywords: H5N8; HPAI; South Korea; mean bird lethal dose