title: Distinguishing N-acetylneuraminic acid linkage isomers on glycopeptides by ion mobility-mass spectrometry.
abstract: Differentiating the structure of isobaric glycopeptides represents a major challenge for mass spectrometry-based characterisation techniques. Here we show that the regiochemistry of the most common N-acetylneuraminic acid linkages of N-glycans can be identified in a site-specific manner from individual glycopeptides using ion mobility-mass spectrometry analysis of diagnostic fragment ions.
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany. daniel.kolarich@mpikg.mpg.de and Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany. kevin.pagel@fu-berlin.de.
authors.forename: H
authors.initials: H
authors.lastname: Hinneburg
authors.affiliation: Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany. kevin.pagel@fu-berlin.de and Fritz Haber Institute of the Max Planck Society, 14195 Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hofmann
authors.affiliation: Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, OX1 3QZ, Oxford, UK.
authors.forename: W B
authors.initials: WB
authors.lastname: Struwe
authors.affiliation: Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Thader
authors.affiliation: Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria.
authors.forename: F
authors.initials: F
authors.lastname: Altmann
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany. daniel.kolarich@mpikg.mpg.de.
authors.forename: D
authors.initials: D
authors.lastname: Varón Silva
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany. daniel.kolarich@mpikg.mpg.de and Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany. kevin.pagel@fu-berlin.de.
authors.forename: P H
authors.initials: PH
authors.lastname: Seeberger
authors.affiliation: Department of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany. kevin.pagel@fu-berlin.de and Fritz Haber Institute of the Max Planck Society, 14195 Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Pagel
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany. daniel.kolarich@mpikg.mpg.de.
authors.forename: D
authors.initials: D
authors.lastname: Kolarich
mesh_terms: D006020:Glycopeptides / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; D019158:N-Acetylneuraminic Acid