title: Survival and Growth of Vibrio cholerae, Escherichia coli, and Salmonella Spp. in Well Water Used for Drinking Purposes in Garoua (North Cameroon).
abstract: The ability of strains of faecal bacteria (Vibrio cholerae, Escherichia coli ATCC 25922, and four strains of Salmonella isolated, resp., from well water, pig, poultry, and human urine in Garoua) to survive or grow in well water microcosms was compared. Water samples were obtained from two wells in Garoua (north Cameroun). Autoclaving at 121°C for 15 min and filtration through 0.2 µm filter were used to make microcosms. Microcosms were constituted of unfiltered-autoclaved, filtered-nonautoclaved, and filtered-autoclaved well waters. Bacterial strains were inoculated at initial cell concentration of 3 Log10CFU/mL. All strains were able to survive/grow in used microcosms, and a maximal concentration of 5.61 Log10CFU/mL was observed. Survival abilities were strain and microcosm dependent. The declines were more pronounced in filtered-nonautoclaved water than in the other microcosms. E. coli and Salmonella sp. (poultry strain) lowered to undetectable levels (<1 Log10CFU/mL) after two days of water storage. V. cholera decreased over time, but surviving cells persisted for longer period in filtered-nonautoclaved water from well W1 (1.91 Log10CFU/mL) and well W2 (2.09 Log10CFU/mL). Competition for nutrients and/or thermolabile antimicrobial substances synthesized by "ultramicrocells" or by the autochthonous bacteria retained by the filter might affect the bacterial survival.
authors.affiliation: University of Maroua, Higher Teachers' Training College, P.O. Box 55, Maroua, Cameroon; University of Yaoundé I, Laboratory of General Biology, P.O. Box 812, Yaoundé, Cameroon.
authors.forename: Moussa
authors.initials: M
authors.lastname: Djaouda
authors.affiliation: Centre Pasteur du Cameroun, Annexe de Garoua, B.P. 921, Garoua, Cameroon.
authors.forename: Bouba
authors.initials: B
authors.lastname: Gaké
authors.affiliation: University of Maroua, Higher Teachers' Training College, P.O. Box 55, Maroua, Cameroon; University of Yaoundé I, Laboratory of General Biology, P.O. Box 812, Yaoundé, Cameroon.
authors.forename: Daniel
authors.initials: D
authors.lastname: Ebang Menye
authors.affiliation: University of Yaoundé I, Laboratory of General Biology, P.O. Box 812, Yaoundé, Cameroon.
authors.forename: Serge Hubert
authors.initials: SH
authors.lastname: Zébazé Togouet
authors.affiliation: University of Yaoundé I, Laboratory of General Biology, P.O. Box 812, Yaoundé, Cameroon.
authors.forename: Moïse
authors.initials: M
authors.lastname: Nola
authors.affiliation: University of Yaoundé I, Laboratory of General Biology, P.O. Box 812, Yaoundé, Cameroon.
authors.forename: Thomas
authors.initials: T
authors.lastname: Njiné