title: Repulsive Guidance Molecule b (RGMb) Is Dispensable for Normal Gonadal Function in Mice.
abstract: Bone morphogenetic protein (BMP) signaling plays an important role in spermatogenesis and follicle development. Our previous studies have shown that repulsive guidance molecule b (RGMb, also known as Dragon) is a coreceptor that enhances BMP2 and BMP4 signaling in several cell types and that RGMb is expressed in spermatocytes and spermatids in the testis and in oocytes of the secondary follicles in the ovary. Here, we demonstrated that specific deletion of Rgmb in germ cells in the testis and ovary did not alter Smad1/5/8 phosphorylation, gonadal structures, and fertility. In addition, ovaries from postnatal global Rgmb knockout mice showed similar structures to the wild-type ovaries. Our results suggest that RGMb is not essential for normal gonadal function.
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Chenling
authors.initials: C
authors.lastname: Meng
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts.
authors.forename: Gordon J
authors.initials: GJ
authors.lastname: Freeman
authors.affiliation: Pioneer Valley Life Science Institute and Department of Veterinary and Animal Science, University of Massachusetts-Amherst, Amherst, Massachusetts.
authors.forename: Alan
authors.initials: A
authors.lastname: Schneyer
authors.affiliation: Program in Membrane Biology, Center for Systems Biology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
authors.forename: Herbert Y
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China School of Biomedical Sciences Core Laboratory, The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen, China xia.yin@cuhk.edu.hk.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal; D005260:Female; D005298:Fertility; D058851:GPI-Linked Proteins / Q000378:metabolism; D008297:Male; D018345:Mice, Knockout; D009124:Muscle Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism / Q000502:physiology* / Q000506:poisoning; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000254:growth & development*; D013737:Testis / Q000033:anatomy & histology / Q000502:physiology*; D000071020:Hemochromatosis Protein
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C483496:Rgma protein, mouse; C483497:Rgmb protein, mouse; C504414:HJV protein, mouse; D000071020:Hemochromatosis Protein
keywords: BMP; Dragon; RGMb; fertility; oocytes; spermatocytes