title: MiR-590-5p Inhibits Oxidized- LDL Induced Angiogenesis by Targeting LOX-1.
abstract: Oxidized low-density lipoprotein (ox-LDL) is, at least in part, responsible for angiogenesis in atherosclerotic regions. This effect of ox-LDL has been shown to be mediated through a specific receptor LOX-1. Here we describe the effect of miR-590-5p on ox-LDL-mediated angiogenesis in in vitro and in vivo settings. Human umbilical vein endothelial cells (HUVECs) were transfected with miR-590-5p mimic or inhibitor followed by treatment with ox-LDL. In other experiments, Marigel plugs were inserted in the mice subcutaneous space. Both in vitro and in vivo studies showed that miR-590-5p mimic (100 nM) inhibited the ox-LDL-mediated angiogenesis (capillary tube formation, cell proliferation and migration as well as pro-angiogenic signals- ROS, MAPKs, pro-inflammatory cytokines and adhesion-related proteins). Of note, miR-590-5p inhibitor (200 nM) had the opposite effects. The inhibitory effect of miR-590-5p on angiogenesis was mediated by inhibition of LOX-1 at translational level. The inhibition of LOX-1 by miR-590-5p was confirmed by luciferase assay. In conclusion, we show that MiR-590-5p inhibits angiogenesis by targeting LOX-1 and suppressing redox-sensitive signals.
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, Anhui, 230032 People's Republic of China.|Department of Medicine, Central Arkansas Veterans Healthcare System and the University of Arkansas for Medical Sciences, Little Rock, AR 72205.|Department of Internal Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022 People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Internal Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022 People's Republic of China.
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022 People's Republic of China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Medicine, Central Arkansas Veterans Healthcare System and the University of Arkansas for Medical Sciences, Little Rock, AR 72205.
authors.forename: Jawahar L
authors.initials: JL
authors.lastname: Mehta
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei, Anhui, 230032 People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D002465:Cell Movement; D049109:Cell Proliferation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors* / Q000502:physiology; D035683:MicroRNAs / Q000502:physiology*; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D051127:Scavenger Receptors, Class E / Q000502:physiology*
chemical_list: D008077:Lipoproteins, LDL; C568221:MIRN590 microRNA, human; D035683:MicroRNAs; C495137:OLR1 protein, human; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein