title: In Vitro Oxidation of Collagen Promotes the Formation of Advanced Oxidation Protein Products and the Activation of Human Neutrophils.
abstract: The accumulation of advanced oxidation protein products (AOPPs) has been linked to several pathological conditions. Here, we investigated collagen as a potential source for AOPP formation and determined the effects of hypochlorous acid (HOCl)-treated collagen (collagen-AOPPs) on human neutrophil activity. We also assessed whether alpha-tocopherol could counteract these effects. Exposure to HOCl increased the levels of collagen-AOPPs. Collagen-AOPPs also stimulated the production of AOPPs, nitric oxide (NO), superoxide radicals (O2(-)), and HOCl by neutrophils. Collagen-AOPPs induced apoptosis and decreased the number of viable cells. Alpha-tocopherol prevented the formation of collagen-AOPPs, strongly inhibited the collagen-AOPP-induced production of O2(-) and HOCl, and increased the viability of neutrophils. Our results suggest that collagen is an important protein that interacts with HOCl to form AOPPs, and consequently, collagen-AOPP formation is related to human neutrophil activation and cell death.
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.|Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Guilherme Vargas
authors.initials: GV
authors.lastname: Bochi
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.|Pharmaceutical Sciences Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Vanessa Dorneles
authors.initials: VD
authors.lastname: Torbitz
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Luízi Prestes
authors.initials: LP
authors.lastname: de Campos
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.|Pharmaceutical Sciences Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Manuela Borges
authors.initials: MB
authors.lastname: Sangoi
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Natieli Flores
authors.initials: NF
authors.lastname: Fernandes
authors.affiliation: Nanosciences Postgraduate Program, Franciscan University Center, Santa Maria, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gomes
authors.affiliation: Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.|Pharmaceutical Sciences Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Maria Beatriz
authors.initials: MB
authors.lastname: Moretto
authors.affiliation: Biogenomic Laboratory, Department of Morphology, Health Science Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Barbisan
authors.affiliation: Biogenomic Laboratory, Department of Morphology, Health Science Center, Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Ivana Beatrice Mânica
authors.initials: IB
authors.lastname: da Cruz
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil. rnmoresco@ufsm.br.|Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil. rnmoresco@ufsm.br.|Pharmaceutical Sciences Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil. rnmoresco@ufsm.br.|Centro de Ciências da Saúde, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Prédio 26, Sala 1401, Camobi, 97105-900, Santa Maria-RS, Brasil. rnmoresco@ufsm.br.
authors.forename: Rafael Noal
authors.initials: RN
authors.lastname: Moresco
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006997:Hypochlorous Acid / Q000737:chemistry / Q000494:pharmacology*; D007249:Inflammation / Q000276:immunology; D018375:Neutrophil Activation / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D009569:Nitric Oxide / Q000096:biosynthesis; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D062107:Advanced Oxidation Protein Products; D013481:Superoxides; D009569:Nitric Oxide; D006997:Hypochlorous Acid; D003094:Collagen; D024502:alpha-Tocopherol
keywords: advanced oxidation protein products; collagen; hypochlorous acid; inflammation; mediators; neutrophils