title: Hippophae rhamnoides L. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma.
abstract: Effects of the phenolic fraction from Hippophae rhamnoides fruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation) and the generation of superoxide anion (O2 (-∙)) in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin) were studied in vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2) or H2O2/Fe (a donor of hydroxyl radicals). The tested fraction of H. rhamnoides (0.5- 50 µg/mL; the incubation time: 15 and 60 min) inhibited lipid peroxidation induced by H2O2 or H2O2/Fe. The H. rhamnoides phenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2 or H2O2/Fe. Moreover, the level of O2 (-∙) in platelets significantly decreased. In comparative experiments, the H. rhamnoides fraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL). The obtained results suggest that H. rhamnoides fruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases.
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, Poland.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Kontek
authors.affiliation: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, 90-236 Łódź, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Malinowska
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Żuchowski
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Stochmal
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002853:Chromatography, Liquid; D005260:Female; D044948:Flavonols / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000032:analysis; D031281:Hippophae / Q000737:chemistry*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010949:Plasma / Q000187:drug effects*
chemical_list: D044948:Flavonols; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts