title: Microsatellites within the feline androgen receptor are suitable for X chromosome-linked clonality testing in archival material.
abstract: Objectives A hallmark of neoplasms is their origin from a single cell; that is, clonality. Many techniques have been developed in human medicine to utilise this feature of tumours for diagnostic purposes. One approach is X chromosome-linked clonality testing using polymorphisms of genes encoded by genes on the X chromosome. The aim of this study was to determine if the feline androgen receptor gene was suitable for X chromosome-linked clonality testing. Methods The feline androgen receptor gene was characterised and used to test clonality of feline lymphomas by PCR and polyacrylamide gel electrophoresis, using archival formalin-fixed, paraffin-embedded material. Results Clonality of the feline lymphomas under study was confirmed and the gene locus was shown to represent a suitable target in clonality testing. Conclusions and relevance Because there are some pitfalls of using X chromosome-linked clonality testing, further studies are necessary to establish this technique in the cat.
authors.affiliation: 1 Department of Veterinary Pathology, Free University of Berlin, Berlin, Germany.
authors.forename: Nadine M
authors.initials: NM
authors.lastname: Farwick
authors.affiliation: 1 Department of Veterinary Pathology, Free University of Berlin, Berlin, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Klopfleisch
authors.affiliation: 1 Department of Veterinary Pathology, Free University of Berlin, Berlin, Germany.
authors.forename: Achim D
authors.initials: AD
authors.lastname: Gruber
authors.affiliation: 2 Chemical and Veterinary Analytical Institute Muensterland-Emscher-Lippe, Muenster, Germany.
authors.forename: Alexander Th A
authors.initials: AT
authors.lastname: Weiss
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000235:genetics* / Q000473:pathology; D002415:Cats; D004285:Dogs; D005091:Exons; D050172:Genes, X-Linked; D006801:Humans; D006965:Hyperplasia / Q000662:veterinary; D008198:Lymph Nodes / Q000473:pathology; D008223:Lymphoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics*; D016415:Sequence Alignment; D014960:X Chromosome / Q000235:genetics*
chemical_list: D011944:Receptors, Androgen