title: Bacillus circulans exopolysaccharide: Production, characterization and bioactivities.
abstract: A bacterium with the ability to produce appreciable amount of exopolysaccharide was isolated from slimy layer of coconut. 16S rDNA analysis identified the organism as Bacillus circulans. EPS production was observed at all stages of culture growth and reached maximum of 0.065mg/ml by 96h, which on further incubation started to decrease. Response Surface Methodology using Box Behnken design has shown the influence of sucrose which was found to be directly proportional to exopolysaccharide production with production reaching 1.09mg/ml. HPLC analysis identified the presence of glucose, mannose, fructose and verbascose and NMR analysis confirmed the presence of glucose, mannose and galactose. Even though the extracted B. circulans EPS did not show appreciable anti-bacterial or anti-fungal activity, it exhibited appreciable antioxidant, anti-inflammatory and anti-tumor activity.
authors.affiliation: Department of Biotechnology, Sathyabama University, Chennai 600 119, India.
authors.forename: R
authors.initials: R
authors.lastname: Vidhyalakshmi
authors.affiliation: Department of Biotechnology, Sathyabama University, Chennai 600 119, India. Electronic address: vnachiyar@gmail.com.
authors.forename: Nachiyar C
authors.initials: NC
authors.lastname: Valli
authors.affiliation: Department of Biotechnology, Sathyabama University, Chennai 600 119, India.
authors.forename: G
authors.initials: G
authors.lastname: Narendra Kumar
authors.affiliation: Department of Biotechnology, Sathyabama University, Chennai 600 119, India.
authors.forename: Swetha
authors.initials: S
authors.lastname: Sunkar
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001407:Bacillus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D056945:Hep G2 Cells; D006801:Humans; D008962:Models, Theoretical; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D014709:Vero Cells
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000970:Antineoplastic Agents; D000975:Antioxidants; D011135:Polysaccharides, Bacterial
keywords: Bacillus circulans; Bioactivities; Characterization; Exopolysaccharides; Optimization