title: [Expressiona of c-Jun and collagens I and III in cultured human skin fibroblasts are affected by infrared ray radiation].
abstract: OBJECTIVE
  To observe the effect of solar infrared ray (IR) radiation on the expressions of c-Jun and collagens I and III in cultured human skin fibroblasts (HSFs) and explore the molecular mechanism by which IR radiation causes aging of the skin.
  
  
  METHODS
  Primarily cultured HSFs exposed to IR radiation were examined for changes of the cell viability with MTT assay. The mRNA and protein expressions of c-Jun and collagens I and III was detected with real-time quantitative PCR and immunocytochemistry.
  
  
  RESULTS
  MTT assay showed that IR irradiation caused inhibition of cell proliferation compared with the control cells. The mRNA and protein expression of collagen I was decreased significantly by IR irradiation with the increase of the irradiation dose (P<0.01). HSFs irradiated by IR for 12 h showed a dose-dependent reduction of the expression of collagen type III mRNA and protein (P<0.05, P<0.01), but the expression increased dose-dependently in response to IR exposure for 24 h (P<0.05 or 0.01). IR irradiation enhanced the mRNA and protein expression of c-Jun in a dose-dependence manner (P<0.05 or 0.01).
  
  
  CONCLUSIONS
  IR irradiation can increase the expression of c-Jun, inhibit the expression of collagen I, and cause disturbance in collagen III expression in human skin fibroblasts, which may be one of the mechanism of IR radiation to initiate and promote skin photoaging.
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. E-mail: medlp@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Rong-Li
authors.initials: RL
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Su
authors.forename: Lin-Li
authors.initials: LL
authors.lastname: Li
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Song
authors.forename: Ning
authors.initials: N
authors.lastname: Lu
authors.forename: Yu-Ze
authors.initials: YZ
authors.lastname: Liu
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D007259:Infrared Rays; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000166:cytology; D015595:Skin Aging; D014466:Ultraviolet Rays
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger