title: [New osmo-regulational promoters in the industrial yeast].
abstract: OBJECTIVE
  The work was aimed at selecting osmo-regulated prompters possessing excellent performance for further research of the industrial yeast Candida glycerinogenes.
  
  
  METHODS
  Promoters PCgPGI, PCgTPI, PCgZWF, PCgSTL1, PCgSTL2 and PCgSTL3 were amplified by PCR and their bioinformatics analysis of stress response elements (STREs) were conducted. We constructed integrative plasmids containing 5.8S rDNA, a fluorescence protein gene gfp and a promoter PCgPGI, PCgTPI, PCgZWF, PCgSTL1, PCgSTL2 or PCgSTL3. The promoters' activities and osmo-regulations were compared according to the results of fluorescence and qRT-PCR.
  
  
  RESULTS
  PCgSTL3 had more STREs, higher transcription level, lager gfp expression and it was more sensitive to stress.
  
  
  CONCLUSIONS
  PCgSTL3 is an excellent induced promoter responding to hyperosmotic stress. Controlled expression of target genes can be realized using PCgSTL3 in the industrial yeast.
authors.forename: Jiali
authors.initials: J
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhuge
authors.forename: Huiying
authors.initials: H
authors.lastname: Fang
authors.forename: Hong
authors.initials: H
authors.lastname: Zong
authors.forename: Xinyao
authors.initials: X
authors.lastname: Lu
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Weiji
authors.initials: W
authors.lastname: Zhang
mesh_terms: D002175:Candida / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007218:Industrial Microbiology; D009995:Osmosis; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins