title: C-type natriuretic peptide improved vitrified-warmed mouse cumulus oocyte complexes developmental competence.
abstract: Cryopreservation of cumulus oocyte complexes (COCs) is important for reproductive medicine. However, the vitrified-warmed COCs have lower maturation rate and subsequent developmental competence compared with fresh COCs. The present study was aimed to evaluate the effects of supplementation of the maturation medium with C-type natriuretic peptide (CNP) on the developmental competence of vitrified-warmed mouse COCs. Addition of CNP to the maturation medium improved the maturation rate and enhanced the developmental competence of vitrified-warmed mouse COCs. The reason may be that vitrified COCs led to a decline in cyclic guanosine monophosphate (cGMP) levels. Furthermore, addition of CNP to the maturation medium elevated cGMP levels of the vitrified-warmed COCs. In conclusion, cryopreservation-associated lower maturation rate and developmental competence of COCs may be ameliorated by CNP during maturation culture after warming.
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Veterinary Medicine, Northwest A＆F University, Yangling, Shaanxi 712100, China. Electronic address: 530934101@qq.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Veterinary Medicine, Northwest A＆F University, Yangling, Shaanxi 712100, China. Electronic address: weiq@nwsuaf.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Veterinary Medicine, Northwest A＆F University, Yangling, Shaanxi 712100, China. Electronic address: 515586982@qq.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Veterinary Medicine, Northwest A＆F University, Yangling, Shaanxi 712100, China. Electronic address: 1316496345@qq.com.
authors.forename: Junbang
authors.initials: J
authors.lastname: Ge
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Veterinary Medicine, Northwest A＆F University, Yangling, Shaanxi 712100, China. Electronic address: zhxiaoe126@126.com.
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Veterinary Medicine, Northwest A＆F University, Yangling, Shaanxi 712100, China. Electronic address: baohuama@163.com.
authors.forename: Baohua
authors.initials: B
authors.lastname: Ma
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D054885:Cumulus Cells / Q000166:cytology* / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism; D051379:Mice; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D009866:Oogenesis / Q000187:drug effects*; D058989:Vitrification
chemical_list: D020098:Natriuretic Peptide, C-Type; D006152:Cyclic GMP
keywords: C-type natriuretic peptide; Cumulus oocyte complexes; Cyclic guanosine monophosphate; Vitrification