title: Mutual association of Broad bean wilt virus 2 VP37-derived tubules and plasmodesmata obtained from cytological observation.
abstract: The movement protein VP37 of broad bean wilt virus 2 (BBWV 2) forms tubules in the plasmodesmata (PD) for the transport of virions between cells. This paper reports a mutual association between the BBWV 2 VP37-tubule complex and PD at the cytological level as determined by transmission electron microscopy. The generation of VP37-tubules within different PD leads to a different occurrence frequency as well as different morphology lines of virus-like particles. In addition, the frequency of VP37-tubules was different between PD found at different cellular interfaces, as well as between single-lined PD and branched PD. VP37-tubule generation also induced structural alterations of PD as well as modifications to the cell wall (CW) in the vicinity of the PD. A structural comparison using three-dimensional (3D) electron tomography (ET), determined that desmotubule structures found in the center of normal PD were absent in PD containing VP37-tubules. Using gold labeling, modification of the CW by callose deposition and cellulose reduction was observable on PD containing VP37-tubule. These cytological observations provide evidence of a mutual association of MP-derived tubules and PD in a natural host, improving our fundamental understanding of interactions between viral MP and PD that result in intercellular movement of virus particles.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.|Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Weina
authors.initials: W
authors.lastname: Shang
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chengke
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Qinfen
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Biology, University of Texas at San Antonio, San Antonio TX 78249, USA.
authors.forename: Garry
authors.initials: G
authors.lastname: Sunter
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.|Center of Analysis and Measurement, Zhejiang University, Hangzhou 310029, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Hong
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002473:Cell Wall / Q000648:ultrastructure / Q000821:virology; D027465:Chenopodium quinoa / Q000648:ultrastructure / Q000821:virology*; D019181:Fabavirus / Q000378:metabolism / Q000648:ultrastructure*; D054884:Host-Pathogen Interactions; D046529:Microscopy, Electron, Transmission; D018515:Plant Leaves / Q000648:ultrastructure / Q000821:virology*; D031425:Plasmodesmata / Q000648:ultrastructure / Q000821:virology*; D021381:Protein Transport; D014764:Viral Proteins / Q000378:metabolism; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D014764:Viral Proteins