title: Analysis of microbial community variation during the mixed culture fermentation of agricultural peel wastes to produce lactic acid.
abstract: Mixed cultures fermentation can be used to convert organic wastes into various chemicals and fuels. This study examined the fermentation performance of four batch reactors fed with different agricultural (orange, banana, and potato (mechanical and steam)) peel wastes using mixed cultures, and monitored the interval variation of reactor microbial communities with 16S rRNA genes using Illumina sequencing. All four reactors produced similar chemical profile with lactic acid (LA) as dominant compound. Acetic acid and ethanol were also observed with small fractions. The Illumina sequencing results revealed the diversity of microbial community decreased during fermentation and a community of largely lactic acid producing bacteria dominated by species of Lactobacillus developed.
authors.affiliation: Renewable Materials Program, Department of Forest, Rangeland and Fire Sciences, University of Idaho, 875 Perimeter Drive MS1132, Moscow, ID 83844, United States.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Karol
authors.initials: K
authors.lastname: Gliniewicz
authors.affiliation: Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Alida T
authors.initials: AT
authors.lastname: Gerritsen
authors.affiliation: Renewable Materials Program, Department of Forest, Rangeland and Fire Sciences, University of Idaho, 875 Perimeter Drive MS1132, Moscow, ID 83844, United States. Electronic address: armandm@uidaho.edu.
authors.forename: Armando G
authors.initials: AG
authors.lastname: McDonald
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D000383:Agriculture / Q000379:methods; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D032084:Citrus sinensis; D005285:Fermentation; D007220:Industrial Waste; D019344:Lactic Acid / Q000096:biosynthesis* / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology*; D028521:Musa; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011198:Solanum tuberosum
chemical_list: D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid; D019342:Acetic Acid
keywords: Illumina sequencing; Lactic acid fermentation; Microbial structure; Peel waste