title: Thermodynamic Features of Structural Motifs Formed by β-L-RNA.
abstract: This is the first report to provide comprehensive thermodynamic and structural data concerning duplex, hairpin, quadruplex and i-motif structures in β-L-RNA series. Herein we confirm that, within the limits of experimental error, the thermodynamic stability of enantiomeric structural motifs is the same as that of naturally occurring D-RNA counterparts. In addition, formation of D-RNA/L-RNA heterochiral duplexes is also observed; however, their thermodynamic stability is significantly reduced in reference to homochiral D-RNA duplexes. The presence of three locked nucleic acid (LNA) residues within the D-RNA strand diminishes the negative effect of the enantiomeric, complementary L-RNA strand in the formation of heterochiral RNA duplexes. Similar behavior is also observed for heterochiral LNA-2'-O-methyl-D-RNA/L-RNA duplexes. The formation of heterochiral duplexes was confirmed by 1H NMR spectroscopy. The CD curves of homochiral L-RNA structural motifs are always reversed, whereas CD curves of heterochiral duplexes present individual features dependent on the composition of chiral strands.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Szabat
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Gudanis
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
authors.forename: Weronika
authors.initials: W
authors.lastname: Kotkowiak
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Gdaniec
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Kierzek
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Pasternak
mesh_terms: D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D059372:Nucleotide Motifs; D009841:Oligonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D020871:RNA Stability; D013816:Thermodynamics
chemical_list: D009841:Oligonucleotides; C477371:locked nucleic acid; D012313:RNA