title: Caffeine inhibits TGFβ activation in epithelial cells, interrupts fibroblast responses to TGFβ, and reduces established fibrosis in ex vivo precision-cut lung slices.
abstract: Caffeine is a commonly used food additive found naturally in many products. In addition to potently stimulating the central nervous system caffeine is able to affect various systems within the body including the cardiovascular and respiratory systems. Importantly, caffeine is used clinically to treat apnoea and bronchopulmonary dysplasia in premature babies. Recently, caffeine has been shown to exhibit antifibrotic effects in the liver in part through reducing collagen expression and deposition, and reducing expression of the profibrotic cytokine TGFβ. The potential antifibrotic effects of caffeine in the lung have not previously been investigated. Using a combined in vitro and ex vivo approach we have demonstrated that caffeine can act as an antifibrotic agent in the lung by acting on two distinct cell types, namely epithelial cells and fibroblasts. Caffeine inhibited TGFβ activation by lung epithelial cells in a concentration-dependent manner but had no effect on TGFβ activation in fibroblasts. Importantly, however, caffeine abrogated profibrotic responses to TGFβ in lung fibroblasts. It inhibited basal expression of the α-smooth muscle actin gene and reduced TGFβ-induced increases in profibrotic genes. Finally, caffeine reduced established bleomycin-induced fibrosis after 5 days treatment in an ex vivo precision-cut lung slice model. Together, these findings suggest that there is merit in further investigating the potential use of caffeine, or its analogues, as antifibrotic agents in the lung.
authors.affiliation: Division of Respiratory Medicine, Nottingham City Hospital, University of Nottingham, Nottingham, UK.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Tatler
authors.affiliation: UCL Respiratory Centre for Inflammation and Tissue Repair, University College London, London, UK.
authors.forename: Josephine
authors.initials: J
authors.lastname: Barnes
authors.affiliation: Division of Respiratory Medicine, Nottingham City Hospital, University of Nottingham, Nottingham, UK.
authors.forename: Anthony
authors.initials: A
authors.lastname: Habgood
authors.affiliation: Division of Respiratory Medicine, Nottingham City Hospital, University of Nottingham, Nottingham, UK.
authors.forename: Amanda
authors.initials: A
authors.lastname: Goodwin
authors.affiliation: UCL Respiratory Centre for Inflammation and Tissue Repair, University College London, London, UK.
authors.forename: Robin J
authors.initials: RJ
authors.lastname: McAnulty
authors.affiliation: Division of Respiratory Medicine, Nottingham City Hospital, University of Nottingham, Nottingham, UK.
authors.forename: Gisli
authors.initials: G
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D011658:Pulmonary Fibrosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D016212:Transforming Growth Factor beta; D002110:Caffeine
keywords: Idiopathic pulmonary fibrosis