title: Microscopy and Microanalysis of Blood in a Snake Head Fish, Channa gachua Exposed to Environmental Pollution.
abstract: Conventional and highly sophisticated analytical methods (Cyria et al., 1989; Massar et al., 2012a) were used to analyze micro-structural and micro-analytical aspects of the blood of snake head fish, Channa gachua, exposed to municipal wastes and city garbage. Red (RBC) and white blood cell (WBC) counts and hemhemoglobin content were found to be higher in pollution affected fish as compared with control. Scanning electron microscopy revealed the occurrence of abnormal erythrocytes such as crenated cells, echinocytes, lobopodial projections, membrane internalization, spherocytes, ruptured cells, contracted cells, depression, and uneven elongation of erythrocyte membranes in fish inhabiting the polluted sites. Energy-dispersive X-ray spectroscopy (EDS) revealed the presence of silicon and lead in the RBCs of pollution affected fish. Significance of the study includes the highly sophisticated analytical approach, which revealed the aforementioned micro-structural abnormalities.
authors.affiliation: 1Department of Zoology,Shillong College,Shillong-793003,Meghalaya,India.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Pala
authors.affiliation: 2Electron Microscope Division, Sophisticated Analytical Instrument Facility,North Eastern Hill University,Shillong-793022,Meghalaya,India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Dey
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D001774:Blood Chemical Analysis; D004787:Environmental Pollution; D004906:Erythrocyte Count; D005399:Fishes; D006454:Hemoglobins / Q000032:analysis; D007854:Lead / Q000032:analysis; D007958:Leukocyte Count; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D012825:Silicon / Q000032:analysis; D013052:Spectrometry, X-Ray Emission
chemical_list: D006454:Hemoglobins; D007854:Lead; D012825:Silicon
keywords: Channa gachua; city garbage; erythrocytes; hill stream