title: Ultrasensitive Profiling of Metabolites Using Tyramine-Functionalized Graphene Quantum Dots.
abstract: Graphene quantum dots (GQDs) are emerging fluorescence reporters attractive for optical sensing, owing to their high photostability, highly tunable photoluminescence, molecular size, atomically thin structure, biocompatibility, and ease of functionalization. Herein, we present a fluorometric sensing platform based on tyramine-functionalized GQDs, which is able to detect a spectrum of metabolites with high sensitivity and specificity. Furthermore, multiparametric blood analysis (glucose, cholesterol, L-lactate, and xanthine) is demonstrated. This convenient metabolite profiling technique could be instrumental for diagnosis, study, and management of metabolic disorders and associated diseases, such as diabetes, obesity, lactic acidosis, gout, and hypertension.
authors.affiliation: Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Aung
authors.initials: A
authors.lastname: Than
authors.affiliation: Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Xuewan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.|Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School , 8 College Road, Singapore 169857, Singapore.
authors.forename: Shaohai
authors.initials: S
authors.lastname: Xu
authors.affiliation: Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School , 8 College Road, Singapore 169857, Singapore.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Duan
authors.affiliation: Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Chenjie
authors.initials: C
authors.lastname: Xu
authors.affiliation: Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University , 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D005470:Fluorometry / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D019344:Lactic Acid / Q000097:blood; D051379:Mice; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D014439:Tyramine / Q000737:chemistry*; D019820:Xanthine / Q000097:blood
chemical_list: D001786:Blood Glucose; D019820:Xanthine; D019344:Lactic Acid; D006108:Graphite; D002784:Cholesterol; D014439:Tyramine
keywords: fluorometric biosensing; graphene quantum dots; metabolite detection; photoluminescence quenching