title: Three-Dimensional Characterization of Mechanical Interactions between Endothelial Cells and Extracellular Matrix during Angiogenic Sprouting.
abstract: We studied the three-dimensional cell-extracellular matrix interactions of endothelial cells that form multicellular structures called sprouts. We analyzed the data collected in-situ from angiogenic sprouting experiments and identified the differentiated interaction behavior exhibited by the tip and stalk cells. Moreover, our analysis of the tip cell lamellipodia revealed the diversity in their interaction behavior under certain conditions (e.g., when the heading of a sprout is switched approximately between the long-axis direction of two different lamellipodia). This study marks the first time that new characteristics of such interactions have been identified with shape changes in the sprouts and the associated rearrangements of collagen fibers. Clear illustrations of such changes are depicted in three-dimensional views.
authors.affiliation: Department of Mechanical Engineering, National University of Singapore, Singapore.|BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology Program, Singapore.
authors.forename: Yue
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Mechanical Engineering, National University of Singapore, Singapore.|BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology Program, Singapore.
authors.forename: Sahan C B
authors.initials: SC
authors.lastname: Herath
authors.affiliation: Department of Electrical and Electronic Engineering Science, University of Johannesburg, South Africa.
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Wang
authors.affiliation: Division of Bioengineering, Nanyang Technological University, Singapore.
authors.forename: Dong-an
authors.initials: DA
authors.lastname: Wang
authors.affiliation: BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology Program, Singapore.|Department of Mechanical Engineering, Massachusetts Institute of Technology, USA.
authors.forename: H Harry
authors.initials: HH
authors.lastname: Asada
authors.affiliation: Department of Mechanical Engineering, National University of Singapore, Singapore.|BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology Program, Singapore.
authors.forename: Peter C Y
authors.initials: PC
authors.lastname: Chen
mesh_terms: D001696:Biomechanical Phenomena; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement; D003094:Collagen / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D042783:Endothelial Cells / Q000378:metabolism / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D021621:Imaging, Three-Dimensional; D056656:Lab-On-A-Chip Devices; D018919:Neovascularization, Physiologic; D061848:Optical Imaging; D011554:Pseudopodia / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D003094:Collagen