title: Cytotoxic evaluation of natural and synthetic callipeltins: a revision of cytotoxicity of callipeltin B.
abstract: The cytotoxic evaluation of natural and synthetic callipeltins is described. Cyclic depsipeptide callipeltin B and linear peptides callipeltins E and M synthesized by us showed no cytotoxic activity. In contrast, natural callipeltin B purified from Callipelta sp. showed CC50 = 130 μM against Hela cells. We found that purified callipeltin B included the contamination of callipeltins C and H at a ratio of approximately 15%. These results suggested that the cytotoxicity of natural callipeltin B was derived from callipeltins C and H.
authors.affiliation: a Department of Biochemical Engineering, Graduate School of Science and Technology , Yamagata University , Yonezawa , Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Kikuchi
authors.affiliation: a Department of Biochemical Engineering, Graduate School of Science and Technology , Yamagata University , Yonezawa , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Konno
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D047630:Depsipeptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D011161:Porifera / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D003603:Cytotoxins; D047630:Depsipeptides; D009842:Oligopeptides; D010456:Peptides, Cyclic; C516600:callipeltin B; C520377:callipeltin E
keywords: callipeltin; cyclic depsipeptides; cytotoxicity