title: Studying solutions at high shear rates: a dedicated microfluidics setup.
abstract: The development of a dedicated small-angle X-ray scattering setup for the investigation of complex fluids at different controlled shear conditions is reported. The setup utilizes a microfluidics chip with a narrowing channel. As a consequence, a shear gradient is generated within the channel and the effect of shear rate on structure and interactions is mapped spatially. In a first experiment small-angle X-ray scattering is utilized to investigate highly concentrated protein solutions up to a shear rate of 300000 s(-1). These data demonstrate that equilibrium clusters of lysozyme are destabilized at high shear rates.
authors.affiliation: Institute for Materials Research, Helmholtz-Zentrum Geestacht: Centre for Materials and Coast Research, Max-Planck-Strasse 1, Geesthacht 21502, Germany.
authors.forename: D C F
authors.initials: DC
authors.lastname: Wieland
authors.affiliation: Institute for Materials Research, Helmholtz-Zentrum Geestacht: Centre for Materials and Coast Research, Max-Planck-Strasse 1, Geesthacht 21502, Germany.
authors.forename: V M
authors.initials: VM
authors.lastname: Garamus
authors.affiliation: Institute for Materials Research, Helmholtz-Zentrum Geestacht: Centre for Materials and Coast Research, Max-Planck-Strasse 1, Geesthacht 21502, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Zander
authors.affiliation: Institute for Materials Research, Helmholtz-Zentrum Geestacht: Centre for Materials and Coast Research, Max-Planck-Strasse 1, Geesthacht 21502, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Krywka
authors.affiliation: Department of Chemistry, Surface and Corrosion Science, KTH Royal Institute of Technology, Drottning Kristinas väg 51, Stockholm 10044, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Surface and Corrosion Science, KTH Royal Institute of Technology, Drottning Kristinas väg 51, Stockholm 10044, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Dedinaite
authors.affiliation: Department of Chemistry, Surface and Corrosion Science, KTH Royal Institute of Technology, Drottning Kristinas väg 51, Stockholm 10044, Sweden.
authors.forename: P M
authors.initials: PM
authors.lastname: Claesson
authors.affiliation: Institute for Materials Research, Helmholtz-Zentrum Geestacht: Centre for Materials and Coast Research, Max-Planck-Strasse 1, Geesthacht 21502, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Willumeit-Römer
mesh_terms: D044085:Microfluidics; D012542:Scattering, Radiation
keywords: aggregation; beamline setup; complex solutions; lysozyme; microfluidics; protein interaction; rheology; shear; small-angle X-ray scattering (SAXS)