title: Expression of the innate immune receptor LILRB5 on monocytes is associated with mycobacteria exposure.
abstract: Antigen presenting cells (APC) are critical components of innate immunity and consequently shape the adaptive response. Leukocyte Ig Like Receptors (LILR) are innate immune receptors predominantly expressed on myeloid cells. LILR can influence the antigen presenting phenotype of monocytic cells to determine the nature of T cell responses in infections including Mycobaterium leprae. We therefore investigated the relevance of LILR in the context of Mycobacterium tuberculosis. Real-time PCR studies indicated that the transcriptional profile of the orphan receptor LILRB5 was significantly up-regulated following exposure to mycobacteria. Furthermore, LILRA1 and LILRB5 were able to trigger signalling through direct engagement of mycobacteria using tranfectant cells incorporating a reporter system. We describe for the first time the expression of this receptor on T cells, and highlight the potential relevance to mycobacterial recognition. Furthermore, we demonstrate that crosslinking of this receptor on T cells increases proliferation of cytotoxic, but not helper, T cells.
authors.affiliation: Institute for Infection and Immunity, St George's, University of London, Cranmer Terrace, London, SW17 0RE.|TB Research Group, Animal and Plant Health Agency, Weybridge, New Haw, KT15 3NB, UK.
authors.forename: Louise E
authors.initials: LE
authors.lastname: Hogan
authors.affiliation: Immunology Division, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP.
authors.forename: Des C
authors.initials: DC
authors.lastname: Jones
authors.affiliation: Institute for Infection and Immunity, St George's, University of London, Cranmer Terrace, London, SW17 0RE.
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Allen
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001500:BCG Vaccine / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007113:Immunity, Innate; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D014611:Vaccination
chemical_list: D015703:Antigens, CD; D001500:BCG Vaccine; C527700:LILRB5 protein, human; D011971:Receptors, Immunologic