title: ULTRASTRUCTURAL CHANGES IN Schistosoma mansoni MALE WORMS AFTER in vitro INCUBATION WITH THE ESSENTIAL OIL OF Mentha x villosa Huds.
abstract: BACKGROUND
  The essential oil Mentha x villosa (MVEO) has a wide range of actions, including antibacterial, antifungal, antiprotozoal and schistosomicidal actions. The present study aimed to investigate the ultrastructural changes of MVEO on the tegument of adult Schistosoma mansoni.
  
  
  METHODS
  Different concentrations of MVEO were tested on S. mansoni adult worms in vitro. Ultrastructural changes on the tegument of these adult worms were evaluated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
  
  
  RESULTS
  The MVEO caused the death of all worms at 500 μg mL(-1) after 24 h. After 24h of 500 μg mL(-1) MVEO treatment, bubble lesions were observed over the entire body of worms and they presented loss of tubercles in some regions of the ventral portion. In the evaluation by TEM, S. mansoni adult worms treated with MVEO, 500 μg mL(-1), presented changes in the tegument and vacuoles in the syncytial matrix region. Glycogen granules close to the muscle fibers were visible.
  
  
  CONCLUSIONS
  The ability of MVEO to cause extensive ultrastructural damage to S. mansoni adult worms correlates with its schistosomicidal effects and confirms earlier findings with S. mansoni.
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
authors.forename: Thiago José
authors.initials: TJ
authors.lastname: Matos-Rocha
authors.affiliation: Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: Marília Gabriela dos Santos
authors.initials: MG
authors.lastname: Cavalcanti
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
authors.forename: Dyana Leal
authors.initials: DL
authors.lastname: Veras
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
authors.forename: Ana Paula Sampaio
authors.initials: AP
authors.lastname: Feitosa
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
authors.forename: Gabriel Gazzoni Araújo
authors.initials: GG
authors.lastname: Gonçalves
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
authors.forename: Nairomberg Cavalcanti
authors.initials: NC
authors.lastname: Portela-Junior
authors.affiliation: Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: Ana Silvia Suassuna Carneiro
authors.initials: AS
authors.lastname: Lúcio
authors.affiliation: Universidade Federal de Pernambuco, Recife, Pernambuco, Brasil.
authors.forename: Anekécia Lauro da
authors.initials: AL
authors.lastname: Silva
authors.affiliation: Universidade Federal de Pernambuco, Recife, Pernambuco, Brasil.
authors.forename: Rafael José Ribeiro
authors.initials: RJ
authors.lastname: Padilha
authors.affiliation: Centro de Recursos Genéticos Vegetais, Biologia Molecular e Fitoquímica, Instituto Agronômico de Campinas, Campinas, São Paulo, Brasil.
authors.forename: Márcia Ortiz Mayo
authors.initials: MO
authors.lastname: Marques
authors.affiliation: Universidade Federal da Paraíba, João Pessoa, PB, Brasil.
authors.forename: José Maria
authors.initials: JM
authors.lastname: Barbosa-Filho
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Alves
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
authors.forename: Fábio André
authors.initials: FA
authors.lastname: Brayner
mesh_terms: D000818:Animals; D008297:Male; D027541:Mentha / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009822:Oils, Volatile / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000648:ultrastructure*; D012556:Schistosomicides / Q000494:pharmacology*
chemical_list: D009822:Oils, Volatile; D012556:Schistosomicides