title: Meloxicam-loaded nanocapsules as an alternative to improve memory decline in an Alzheimer's disease model in mice: involvement of Na(+), K(+)-ATPase.
abstract: The objective of this study was to investigate the effect of meloxicam-loaded nanocapsules (M-NC) on the treatment of the memory impairment induced by amyloid β-peptide (aβ) in mice. The involvement of Na(+), K(+)-ATPase and cyclooxygenase-2 (COX-2) activities in the hippocampus and cerebral cortex was also evaluated. Mice received aβ (3 nmol/ 3 μl/ per site, intracerebroventricular) or vehicle (3 μl/ per site, i.c.v.). The next day, the animals were treated with blank nanocapsules (17 mL/kg) or M-NC (5 mg/kg) or free meloxicam (M-F) (5 mg/kg). Treatments were performed every other day, until the twelfth day. Animals were submitted to the behavioral tasks (open-field, object recognition, Y-maze and step-down inhibitory avoidance tasks) from the twelfth day. Na(+), K(+)-ATPase and COX-2 activities were performed in hippocampus and cerebral cortex. aβ caused a memory deficit, an inhibition of the hippocampal Na(+), K(+)-ATPase activity and an increase in the hippocampal COX-2 activity. M-NC were effective against all behavioral and biochemical alterations, while M-F restored only the COX-2 activity. In conclusion, M-NC were able to reverse the memory impairment induced by aβ, and Na(+), K(+)-ATPase is involved in the effect of M-NC.
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Santa Maria, CEP 97010-032, RS, Brazil.
authors.forename: Francine R
authors.initials: FR
authors.lastname: Ianiski
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Santa Maria, CEP 97010-032, RS, Brazil.
authors.forename: Catiane B
authors.initials: CB
authors.lastname: Alves
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Santa Maria, CEP 97010-032, RS, Brazil.
authors.forename: Carla F
authors.initials: CF
authors.lastname: Ferreira
authors.affiliation: Programa de Pós-Graduação em Nanociências, Centro de Ciências Tecnológicas, Centro Universitário Franciscano, Santa Maria, CEP 97010-032, RS, Brazil.
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Rech
authors.affiliation: Grupo de pesquisa em Neurobiotecnologia - GPN, Centro de Desenvolvimento Tecnológico, Unidade Biotecnologia, Universidade Federal de Pelotas, Pelotas, CEP: 96010-900, RS, Brazil.
authors.forename: Lucielli
authors.initials: L
authors.lastname: Savegnago
authors.affiliation: Grupo de pesquisa em Neurobiotecnologia - GPN, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Capão do Leão, Pelotas, CEP 96010-900, RS, Brazil. ethelwilhelm@yahoo.com.br.
authors.forename: Ethel A
authors.initials: EA
authors.lastname: Wilhelm
authors.affiliation: Grupo de pesquisa em Neurobiotecnologia - GPN, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Capão do Leão, Pelotas, CEP 96010-900, RS, Brazil. cristiane_luchese@yahoo.com.br.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Luchese
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D000077239:Meloxicam; D008568:Memory / Q000187:drug effects; D008569:Memory Disorders / Q000188:drug therapy* / Q000378:metabolism; D051379:Mice; D053769:Nanocapsules / Q000008:administration & dosage*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013843:Thiazines / Q000008:administration & dosage / Q000627:therapeutic use*; D013844:Thiazoles / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D053769:Nanocapsules; D013843:Thiazines; D013844:Thiazoles; D051546:Cyclooxygenase 2; D000254:Sodium-Potassium-Exchanging ATPase; D000077239:Meloxicam
keywords: Amyloid-β; Cyclooxygenase; Drug-delivery; Meloxicam; Nanoparticles