title: Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3.
abstract: The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons. The SCUBE3 gene contained a 2895-base pair open reading frame that encoded a peptide of 965 amino acids. Comparison of the deduced amino acid sequences of porcine SCUBE3 with those of human, mouse, zebrafish, and rat showed 96, 95, 73, and 95% identities, respectively. Porcine SCUBE3 mRNA expression levels were highest in the backfat, bone marrow, and cartilage tissues. Copy number variation was detected in porcine SCUBE3 and validated by real-time quantitative polymerase chain reaction. Different copy number variations were present in randomly selected individuals and may, therefore, be a good marker for identifying phenotypic traits. Our findings provide a basis for further investigation of the functions and regulatory mechanisms of SCUBE3 in pigs.
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: L G
authors.initials: LG
authors.lastname: Wang
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: L C
authors.initials: LC
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Yan
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: K B
authors.initials: KB
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Liang
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Pu
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture of China, Institute of Animal Science,Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: L X
authors.initials: LX
authors.lastname: Wang
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism; D003001:Cloning, Molecular; D056915:DNA Copy Number Variations; D015870:Gene Expression; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011956:Receptors, Cell Surface