title: Interleukin-17A Gene Variability in Patients with Type 1 Diabetes Mellitus and Chronic Periodontitis: Its Correlation with IL-17 Levels and the Occurrence of Periodontopathic Bacteria.
abstract: Interleukin-17 contributes to the pathogenesis of type 1 diabetes mellitus (T1DM) and chronic periodontitis (CP). We analyzed IL-17A -197A/G and IL-17F +7488C/T polymorphisms in T1DM and CP and determined their associations with IL-17 production and occurrence of periopathogens. Totally 154 controls, 125 T1DM, and 244 CP patients were genotyped using 5' nuclease TaqMan(®) assays. Bacterial colonization was investigated by a DNA-microarray kit. Production of IL-17 after in vitro stimulation of mononuclear cells by mitogens and bacteria was examined by the Luminex system. Although no differences in the allele/genotype frequencies between patients with CP and T1DM + CP were found, the IL-17A -197 A allele increased the risk of T1DM (P < 0.05). Levels of HbA1c were significantly elevated in carriers of the A allele in T1DM patients (P < 0.05). Production of IL-17 by mononuclear cells of CP patients (unstimulated/stimulated by Porphyromonas gingivalis) was associated with IL-17A A allele (P < 0.05). IL-17A polymorphism increased the number of Tannerella forsythia and Treponema denticola in patients with CP and T1DM + CP, respectively (P < 0.05). IL-17A gene variability may influence control of T1DM and the "red complex" bacteria occurrence in patients with CP and T1DM + CP. Our findings demonstrated the functional relevance of the IL-17A polymorphism with higher IL-17 secretion in individuals with A allele.
authors.affiliation: Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic; Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
authors.forename: Petra
authors.identifier: 0000-0003-0953-3615
authors.initials: P
authors.lastname: Borilova Linhartova
authors.affiliation: Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic; Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kastovsky
authors.affiliation: Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Lucanova
authors.affiliation: Institute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, 128 08 Prague, Czech Republic.
authors.forename: Jirina
authors.initials: J
authors.lastname: Bartova
authors.affiliation: Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic.
authors.forename: Hana
authors.identifier: 0000-0002-0727-0180
authors.initials: H
authors.lastname: Poskerova
authors.affiliation: Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic.
authors.forename: Jan
authors.identifier: 0000-0003-2341-9771
authors.initials: J
authors.lastname: Vokurka
authors.affiliation: Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic.
authors.forename: Antonin
authors.initials: A
authors.lastname: Fassmann
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
authors.forename: Katerina
authors.identifier: 0000-0001-9548-0630
authors.initials: K
authors.lastname: Kankova
authors.affiliation: Clinic of Stomatology, Institutions Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, 656 91 Brno, Czech Republic; Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
authors.forename: Lydie
authors.identifier: 0000-0002-7610-8929
authors.initials: L
authors.lastname: Izakovicova Holla
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000097:blood* / Q000235:genetics* / Q000382:microbiology; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020381:Interleukin-17 / Q000097:blood* / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: C508717:IL17A protein, human; C440021:IL17F protein, human; D020381:Interleukin-17