title: A Small Molecule for Controlled Generation of Peroxynitrite.
abstract: 2-Methyl-3-[1-(N,N-dimethylamino)diazen-1-ium-1,2-diol-2-ato-methyl]-naphthalene-1,4-dione 1 (HyPR-1), a small molecule containing a superoxide generator strategically linked to a diazeniumdiolate-based nitric oxide donor, is reported. Evidence for HyPR-1's ability to generate peroxynitrite in the presence of an enzyme as well as enhance peroxynitrite within cells is provided. The utility of this tool in generating peroxynitrite for cellular studies is demonstrated.
authors.affiliation: Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Maharashtra, India.
authors.forename: Vinayak S
authors.initials: VS
authors.lastname: Khodade
authors.affiliation: Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Maharashtra, India.
authors.forename: Apoorva
authors.initials: A
authors.lastname: Kulkarni
authors.affiliation: Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Maharashtra, India.
authors.forename: Ayantika
authors.initials: A
authors.lastname: Sen Gupta
authors.affiliation: Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Maharashtra, India.
authors.forename: Kundan
authors.initials: K
authors.lastname: Sengupta
authors.affiliation: Indian Institute of Science Education and Research Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Maharashtra, India.
authors.forename: Harinath
authors.initials: H
authors.lastname: Chakrapani
mesh_terms: D001391:Azo Compounds / Q000737:chemistry; D015394:Molecular Structure; D009285:Naphthoquinones / Q000138:chemical synthesis* / Q000737:chemistry; D020030:Nitric Oxide Donors / Q000737:chemistry; D030421:Peroxynitrous Acid / Q000737:chemistry*
chemical_list: D001391:Azo Compounds; D009285:Naphthoquinones; D020030:Nitric Oxide Donors; C452539:diazeniumdiolate; D030421:Peroxynitrous Acid