title: Optimization of bacterial cellulose production by Gluconacetobacter xylinus using carob and haricot bean.
abstract: Bacterial cellulose (BC) can be used in medical, biomedical, electronic, food, and paper industries because of its unique properties distinguishing it from plant cellulose. BC production was statistically optimized by Gluconacetobacter xylinus strain using carob and haricot bean (CHb) medium. Eight parameters were evaluated by Plackett-Burman Design and significant three parameters were optimized by Central Composite Design. Optimal conditions for production of BC in static culture were found as: 2.5g/L carbon source, 2.75g/L protein source, 9.3% inoculum ratio, 1.15g/L citric acid, 2.7g/L Na2HPO4, 30°C incubation temperature, 5.5 initial pH, and 9days of incubation. This study reveals that BC production can be carried out using carob and haricot bean extracts as carbon and nitrogen sources, and CHb medium has higher buffering capacity compared to Hestrin and Schramm media. Model obtained from this study is used to predict and optimize BC production yield using CHb medium.
authors.affiliation: Ege University, Graduate School of Natural and Applied Science, Department of Biomedical Technologies, Izmir, Turkey.
authors.forename: Eyup
authors.initials: E
authors.lastname: Bilgi
authors.affiliation: Ege University, Graduate School of Natural and Applied Science, Department of Biomedical Technologies, Izmir, Turkey; Ege University, Application and Research Center for Testing and Analysis (EGE-MATAL), Izmir, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Bayir
authors.affiliation: Ege University, Graduate School of Natural and Applied Science, Department of Biomedical Technologies, Izmir, Turkey; Ege University, Application and Research Center for Testing and Analysis (EGE-MATAL), Izmir, Turkey; Ege University, Department of Bioengineering, Faculty of Engineering, Izmir, Turkey.
authors.forename: Aylin
authors.initials: A
authors.lastname: Sendemir-Urkmez
authors.affiliation: Ege University, Department of Bioengineering, Faculty of Engineering, Izmir, Turkey. Electronic address: esin.hames@ege.edu.tr.
authors.forename: E Esin
authors.initials: EE
authors.lastname: Hames
mesh_terms: D000704:Analysis of Variance; D001671:Biochemistry / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D002482:Cellulose / Q000096:biosynthesis* / Q000648:ultrastructure; D007887:Fabaceae / Q000378:metabolism*; D005685:Galactans / Q000378:metabolism*; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism*; D053149:Plant Gums / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D002241:Carbohydrates; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D011506:Proteins; D002482:Cellulose; C017471:locust bean gum
keywords: Bacterial cellulose; Carob; Gluconacetobacter xylinus; Haricot bean; Statistical optimization