title: Evaluation of the ability of bone marrow derived cells to engraft the kidney and promote renal tubular regeneration in mice following exposure to cisplatin.
abstract: It has been suggested that bone marrow derived stem cells have the ability to engraft the kidney and improve the outcome of severe acute kidney injury (AKI) in mice exposed to high doses of cisplatin, providing hope for cancer patients in whom irreversible renal damage occasionally occurs following the use of this highly effective anti-tumor drug. We tested the therapeutic potential of bone marrow derived cells injected during the acute phase (day 3 after cisplatin administration) of experimentally-induced AKI in C57Bl6/J mice, characterized by massive tubular necrosis, apoptosis, and a low proliferation capacity. We failed to show any benefit of bone marrow derived cells versus a regular homogenate of intact renal cells, or normal saline. Using cell tracers and flow cytometry, we demonstrated that bone marrow derived cells did indeed home to the bone marrow of the recipients but failed to settle in the kidney. Conversely, renal cells homed to injured kidneys. However, neither cell therapy protected the animals against cisplatin-induced death. We therefore question the short-term efficacy of bone marrow derived cells used to repair established injuries of the tubular epithelium.
authors.affiliation: a Inserm UMR_S 1155, "Rare and Common Kidney Diseases, Matrix Remodelling and Tissue Repair", Tenon Hospital , Paris , France ;
authors.forename: Aurélien
authors.initials: A
authors.lastname: Bataille
authors.affiliation: a Inserm UMR_S 1155, "Rare and Common Kidney Diseases, Matrix Remodelling and Tissue Repair", Tenon Hospital , Paris , France ;|b Sorbonne Universités , UPMC Université Paris 6 , Paris , France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Galichon
authors.affiliation: a Inserm UMR_S 1155, "Rare and Common Kidney Diseases, Matrix Remodelling and Tissue Repair", Tenon Hospital , Paris , France ;
authors.forename: Morgane
authors.initials: M
authors.lastname: Wetzstein
authors.affiliation: a Inserm UMR_S 1155, "Rare and Common Kidney Diseases, Matrix Remodelling and Tissue Repair", Tenon Hospital , Paris , France ;
authors.forename: David
authors.initials: D
authors.lastname: Legouis
authors.affiliation: a Inserm UMR_S 1155, "Rare and Common Kidney Diseases, Matrix Remodelling and Tissue Repair", Tenon Hospital , Paris , France ;
authors.forename: Sophie
authors.initials: S
authors.lastname: Vandermeersch
authors.affiliation: a Inserm UMR_S 1155, "Rare and Common Kidney Diseases, Matrix Remodelling and Tissue Repair", Tenon Hospital , Paris , France ;|b Sorbonne Universités , UPMC Université Paris 6 , Paris , France.
authors.forename: Eric
authors.initials: E
authors.lastname: Rondeau
authors.affiliation: a Inserm UMR_S 1155, "Rare and Common Kidney Diseases, Matrix Remodelling and Tissue Repair", Tenon Hospital , Paris , France ;|b Sorbonne Universités , UPMC Université Paris 6 , Paris , France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Hertig
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000601:surgery*; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D001854:Bone Marrow Cells / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002945:Cisplatin / Q000009:adverse effects; D005260:Female; D007668:Kidney / Q000502:physiology; D007684:Kidney Tubules / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012038:Regeneration
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin
keywords: Acute kidney injury; bone marrow cells; bone marrow transplantation; cisplatin; stem cells