title: Influence of endogenous glucagon-like peptide-2 on lipid disorders in mice fed a high-fat diet.
abstract: OBJECTIVE
  The purpose of the present study was to investigate the influence of endogenous glucagon-like peptide-2 (GLP-2) on lipid profile in mice fed a standard diet (STD) or a high-fat diet (HFD).
  
  
  METHODS
  HFD- and age-matched STD mice were injected once a day with GLP-2 (3-33), a GLP-2 receptor (GLP-2R) antagonist, or vehicle for 4 weeks.
  
  
  RESULTS
  HFD mice displayed increased intrahepatic lipid concentration and hepatic steatosis and higher plasma concentrations of cholesterol, LDL, AST, and ALT than STD mice. No difference was observed in lipid fecal elimination. In STD mice, the chronic treatment with GLP-2 (3-33) did not affect any parameter, while in HFD mice, it enhanced plasma triglycerides, cholesterol, ALT, and AST and reduced HDL, it increased intrahepatic lipid concentration, and it worsened the hepatic steatosis degree, without affecting lipid fecal elimination.
  
  
  CONCLUSIONS
  The present results suggest that GLP-2R antagonism worsens lipid disorders in HFD mice, and endogenous GLP-2 might even exert a defensive role against lipid imbalance.
authors.affiliation: a Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF) , Università di Palermo , Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Baldassano
authors.affiliation: a Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF) , Università di Palermo , Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Amato
authors.affiliation: b Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche - Università di Palermo , Italy.|c Istituto Euro-Mediterraneo di Scienza e Tecnologia , Palermo , Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rappa
authors.affiliation: b Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche - Università di Palermo , Italy.|c Istituto Euro-Mediterraneo di Scienza e Tecnologia , Palermo , Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Cappello
authors.affiliation: a Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF) , Università di Palermo , Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Mulè
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D053767:Glucagon-Like Peptide 2 / Q000008:administration & dosage / Q000494:pharmacology*; D008055:Lipids / Q000097:blood*; D008297:Male; D008659:Metabolic Diseases / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D053767:Glucagon-Like Peptide 2; D008055:Lipids; D010446:Peptide Fragments; C451520:glucagon-like peptide-2 (3-33)
keywords: GLP-2; insulin resistance; lipids; liver; obesity