title: Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy.
abstract: Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular- and subcellular-level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA-based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy.
authors.affiliation: Department of Biomedical Engineering, Texas A&M University, College Station, TX, 77843-3120, USA. mzkmzk89@tamu.edu.
authors.forename: Zhaokai
authors.initials: Z
authors.lastname: Meng
authors.affiliation: College of Engineering, Swansea University, Singleton Park, Swansea, SA2 8PP, Wales, United Kingdom.
authors.forename: Sandra C
authors.initials: SC
authors.lastname: Bustamante Lopez
authors.affiliation: College of Engineering, Swansea University, Singleton Park, Swansea, SA2 8PP, Wales, United Kingdom.
authors.forename: Kenith E
authors.initials: KE
authors.lastname: Meissner
authors.affiliation: Department of Biomedical Engineering, Texas A&M University, College Station, TX, 77843-3120, USA.
authors.forename: Vladislav V
authors.initials: VV
authors.lastname: Yakovlev
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002417:Cattle; D004912:Erythrocytes / Q000166:cytology; D042541:Intracellular Space; D055595:Mechanical Phenomena; D008853:Microscopy; D013059:Spectrum Analysis, Raman / Q000379:methods*