title: The features of skin inflammation induced by lupus serum.
abstract: We recently developed a model of lupus serum-induced skin inflammation, which was used to study the pathogenesis of skin injury in systemic lupus erythematosus (SLE). We further characterized the features of lupus serum-induced skin inflammation. This skin inflammation was evident within 3h and lasted for at least two weeks. The skin inflammation was characterized by an influx of monocytic, CD11b+cells and by a scarcity of T and B lymphocytes. Depletion of IgG from the serum abrogated the skin inflammatory response. The skin inflammation was related to lupus patients' skin history but not to SLE disease activity and type of autoantibody. The expression of TNFR1, NF-kB and MCP-1 was increased locally in skin lesions. The TLR9 ligand and lupus serum act synergistically to trigger skin inflammation. These findings suggest that this novel model is valuable for the study of the pathogenesis and therapy of skin injury in SLE.
authors.affiliation: Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA.
authors.forename: Lena
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Lab of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Guangqion
authors.initials: G
authors.lastname: Xu
authors.affiliation: Key Lab of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dou
authors.affiliation: Key Lab of Antibody Techniques of Ministry of Health, Nanjing Medical University, Nanjing, China; Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China. Electronic address: gmdeng@njmu.edu.cn.
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Deng
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000097:blood / Q000494:pharmacology; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D044967:Serum / Q000276:immunology*; D012867:Skin / Q000187:drug effects* / Q000473:pathology
chemical_list: D001323:Autoantibodies; D016207:Cytokines; D007106:Immune Sera; D007074:Immunoglobulin G
keywords: IgG; Lupus serum; Skin inflammation; Systemic lupus erythematosus