title: [Effect of Human Umbilical Cord Mesenchymal Stem Cells on Etoposide-induced Nalm-6 Cell Apoptosis].
abstract: OBJECTIVE
  To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSC) on VP16-induced apoptosis of Nalm-6 cells.
  
  
  METHODS
  hUC-MSC were isolated and identified using morphological observation and flow cytometry, then Nalm-6 cells were treated with hUC-MSC with or without VP16. Apoptosis and cell cycle were assayed by FACS. The mRNA levels of apoptosis-related genes BCL-2, BAX and caspase-3 were detected by quantitative RT-PCR, and the protein levels of BCL-2, BAX and caspase-3 were examined by Western blot.
  
  
  RESULTS
  FACS showed that hUC-MSC inhibited the proliferation and decreased apoptosis of Nalm-6 cells resulted from VP16. The quantitative RT-PCR showed that hUC-MSC increased the mRNA expression level of BCL-2 and decreased the expression level of BAX and caspase-3 (P < 0.05). Western blot showed that the protein expression level of BCL-2 increased, and expression level of BAX and caspase-3 decreased in Nalm-6 cells after co-culture with hUC-MSC (P < 0.05).
  
  
  CONCLUSIONS
  hUC-MSC may protect Nalm-6 cells from apoptosis induced by VP16 through regulation of BCL-2, BAX and caspase-3.
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Jian-Ling
authors.initials: JL
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Zhou
authors.affiliation: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China. E-mail: Shellysdcn@hotmail.com.
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Ju
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques; D005047:Etoposide / Q000009:adverse effects*; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D005047:Etoposide; C505407:CASP3 protein, human; D053148:Caspase 3