title: The coordination of ploidy and cell size differs between cell layers in leaves.
abstract: Growth and developmental processes are occasionally accompanied by multiple rounds of DNA replication, known as endoreduplication. Coordination between endoreduplication and cell size regulation often plays a crucial role in proper organogenesis and cell differentiation. Here, we report that the level of correlation between ploidy and cell volume is different in the outer and inner cell layers of leaves of Arabidopsis thaliana using a novel imaging technique. Although there is a well-known, strong correlation between ploidy and cell volume in pavement cells of the epidermis, this correlation was extremely weak in palisade mesophyll cells. Induction of epidermis cell identity based on the expression of the homeobox gene ATML1 in mesophyll cells enhanced the level of correlation between ploidy and cell volume to near that of wild-type epidermal cells. We therefore propose that the correlation between ploidy and cell volume is regulated by cell identity.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Katagiri
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Hasegawa
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Ushio
authors.initials: U
authors.lastname: Fujikura
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Hoshino
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan sachi@rs.tus.ac.jp tsukaya@bs.s.u-tokyo.ac.jp.
authors.forename: Sachihiro
authors.initials: S
authors.lastname: Matsunaga
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan Bio-Next Project, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Yamate Build. #3, 5-1, Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan sachi@rs.tus.ac.jp tsukaya@bs.s.u-tokyo.ac.jp.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tsukaya
mesh_terms: D017360:Arabidopsis / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D048429:Cell Size; D062951:Endoreduplication / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D058503:Mesophyll Cells / Q000166:cytology* / Q000378:metabolism; D019441:Plant Epidermis / Q000166:cytology / Q000254:growth & development; D018515:Plant Leaves / Q000166:cytology* / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000378:metabolism; D011003:Ploidies
chemical_list: C103790:ATML1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins
keywords: ATML1; Cell volume; Endoreduplication; Epidermis; Mesophyll tissue; Ploidy