title: Extensive hydrolysis of phosphonates as unexpected behaviour of the known His6-organophosphorus hydrolase.
abstract: The catalytic activity of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) in hydrolytic reactions of methylphosphonic acid (MPA) and its monoesters and diesters being decomposition products of R-VX was demonstrated for the first time. The catalytic constants of enzyme in such reactions were determined. The mechanism of C-P bond cleavage in the MPA by His6-OPH was proposed. Such reaction was estimated to be carried out with the soluble and nanocapsulated forms of His6-OPH. His6-OPH was demonstrated to be capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by the chemical detoxification of R-VX and RMs are multi-substrate mixtures for this enzyme. The kinetic model describing the behaviour of His6-OPH in RMs was proposed and was shown to adequately fit experimental points during degradation of the real samples of RMs.
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, Lenin Hills, 1, building 3, Moscow, 119991, Russia.|Institute of Biochemical Physics RAS, Kosygin St. 4, Moscow, 119334, Russia.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Lyagin
authors.affiliation: SMC Technological Center MIET, Proezd No. 4806, 5, Moscow, Zelenograd, 124498, Russia.
authors.forename: Mariia S
authors.initials: MS
authors.lastname: Andrianova
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, Lenin Hills, 1, building 3, Moscow, 119991, Russia. elena_efremenko@list.ru.|Institute of Biochemical Physics RAS, Kosygin St. 4, Moscow, 119334, Russia. elena_efremenko@list.ru.
authors.forename: Elena N
authors.identifier: http://orcid.org/0000-0002-6992-854X
authors.initials: EN
authors.lastname: Efremenko
mesh_terms: D020561:Alphaproteobacteria / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D043303:Aryldialkylphosphatase / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism; D063065:Organophosphonates / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D063065:Organophosphonates; D006639:Histidine; D043303:Aryldialkylphosphatase
keywords: Enzymatic degradation; Hexahistidine-tagged organophosphorus hydrolase; Mechanism; Organophosphorus compound; Phosphonate