title: Sequential VEGF and BMP-2 releasing PLA-PEG-PLA scaffolds for bone tissue engineering: I. Design and in vitro tests.
abstract: Biodegradable PLA-PEG-PLA block copolymers were synthesized with desired backbone structures and molecular weights using PEG20000. Rectangular scaffolds were prepared by freeze drying with or without using NaCl particles. Bone morphogenetic protein (BMP)-2 was loaded to the matrix after the scaffold formation for sustained release while vascular endothelial growth factor (VEGF) was loaded within the pores with gelatin solution. VEGF release was quite fast and almost 60% of it was released in 2 d. However, sequential - sustained released was observed for BMP-2 in the following few months. Corporation of VEGF/BMP-2 couple into the scaffolds increased the cell adhesion and proliferation. Neither significant cytotoxicity nor apoptosis/necrosis were observed.
authors.affiliation: a Department of Bioengineering, Faculty of Engineering and Natural Sciences , Gaziosmanpaşa University , Tokat , Turkey.
authors.forename: Sinan
authors.initials: S
authors.lastname: Eğri
authors.affiliation: b Department of Bioengineering, Faculty of Engineering , Karamanoğlu Mehmetbey University , Karaman , Turkey.
authors.forename: Numan
authors.initials: N
authors.lastname: Eczacıoğlu
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002460:Cell Line; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D003692:Delayed-Action Preparations; D011091:Polyesters; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D011092:Polyethylene Glycols; C033616:poly(lactide); C000595209:polyethylene glycol 1000
keywords: BMP-2 and VEGF controlled release; Biodegradable/biocompatible; PLA-PEG-PLA block copolymers; bone tissue engineering; freeze drying; in vitro tests; salt leaching; scaffolds