title: Synthesis of triazole-linked morpholino oligonucleotides via Cu(I) catalysed cycloaddition.
abstract: Triazole-linked morpholino ((TL)MO) oligonucleic acids were synthesised using the Cu(I) catalysed (3 + 2) azide-alkyne cycloaddition (CuAAC) reaction. The modified DNA analogues were incorporated into 13-mer sequences via solid phase synthesis. UV melting experiments showed that the (TL)MO modification gives higher Tm values than the corresponding (TL)DNA modification.
authors.affiliation: School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. chris.hayes@nottingham.ac.uk.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Palframan
authors.forename: Rima D
authors.initials: RD
authors.lastname: Alharthy
authors.forename: Paulina K
authors.initials: PK
authors.lastname: Powalowska
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hayes
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D060172:Morpholinos / Q000138:chemical synthesis / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D060327:Solid-Phase Synthesis Techniques / Q000379:methods; D014230:Triazoles / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D060172:Morpholinos; D014230:Triazoles; D003300:Copper; D004247:DNA