title: Hyphal tip cytoplasmic organization in four zygomycetous fungi.
abstract: We have examined the hyphal tip structure in four zygomycetous fungi: Mortierella verticillata (Mortierellales), Coemansia reversa (Kickxellales), Mucor indicus and Gilbertella persicaria (Mucorales) using both light and transmission electron microscopy. We have used cryofixation and freeze-substitution methods to preserve fungal hyphae for transmission electron microscopy, which yielded improved preservation of ultrastructural details. Our research has confirmed studies that described the accumulation of secretory vesicles as a crescent at the hyphal apex (i.e. the apical vesicle crescent [AVC]) and provided a more detailed understanding of the vesicle populations. In addition, we have been able to observe the behavior of the AVC during hyphal growth in M. indicus and G. persicaria.
authors.affiliation: School of Life Sciences, Arizona State University, Tempe, Arizona 85287.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Fisher
authors.affiliation: School of Life Sciences, Arizona State University, Tempe, Arizona 85287 robby@asu.edu.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Roberson
mesh_terms: D003593:Cytoplasm / Q000648:ultrastructure*; D005658:Fungi / Q000254:growth & development* / Q000648:ultrastructure; D025301:Hyphae / Q000254:growth & development / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission
keywords: apical vesicle crescent; cryofixation; electron microscopy; hyphal growth; light microscopy; phylogeny; vesicle organization