title: Expression of ARE-binding proteins AUF1 and HuR in follicular adenoma and carcinoma of thyroid gland.
abstract: Both adenylate-uridylate rich elements binding proteins AUF1 and HuR may participate in thyroid carcinoma progression. In this study we investigated the expression of both factors on a protein level with a special focus on follicular adenoma and follicular thyroid carcinoma. By employment of immunofluorescence and western blot on 68 thyroid tissues including 7 goiter, 16 follicular adenoma (4 adenomatous hyperplasia), 19 follicular thyroid carcinomas, 13 papillary thyroid carcinomas and 14 undifferentiated thyroid carcinomas we investigated protein expression of AUF1 and HuR. In addition to previous results we demonstrated that AUF1 and HuR are significantly up-regulated in carcinoma tissues as compared with follicular adenoma or goiter tissues. Furthermore, by evaluation of AUF1 or HuR expression, or combination of both proteins on total tissue lysates, we were able to demonstrate a significant difference between follicular adenoma and follicular thyroid carcinoma. Overexpression of AUF1 and HuR is a common finding observed in thyroid malignancy. Analysis of the tissues obtained by surgical resection as demonstrated in this study is comparable to a fine needle aspiration and in combination with AUF1/HuR immuno-analysis may support the conventional immunohistological investigations. The promising results of this study were performed on relatively small collective, but justify future development of a quick thyroid diagnostic test on larger cohort of the patients, especially for thyroid samples which are inadequate for histological examinations.
authors.forename: B
authors.initials: B
authors.lastname: Trojanowicz
authors.forename: C
authors.initials: C
authors.lastname: Sekulla
authors.forename: H
authors.initials: H
authors.lastname: Dralle
authors.forename: C
authors.initials: C
authors.lastname: Hoang-Vu
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000378:metabolism* / Q000473:pathology; D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D044963:Biopsy, Fine-Needle; D015153:Blotting, Western; D000067780:ELAV-Like Protein 1 / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D006042:Goiter / Q000378:metabolism / Q000473:pathology; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000096:biosynthesis*; D006801:Humans; D009367:Neoplasm Staging; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; C000630986:HNRNPD protein, human; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D
keywords: ARE; AUF1; FTC.; HuR; follicular adenoma; thyroid carcinoma