title: [Antigenemia and real time polymerase chain reaction for diagnosis of cytomegalovirus disease in HIV infected adults].
abstract: BACKGROUND
  Cytomegalovirus (CMV) infection is frequent in HIV adults. It is unknown usefulness of quantitative methods for diagnosing the CMV disease in Chilean patients.
  
  
  OBJECTIVE
  To determine the performance of antigenemia and real time polymerase chain reaction (rtPCR) in the diagnosis of CMV disease in Chilean HIV adults.
  
  
  METHODS
  Detection of CMV by viral isolation (AVR), antigenemia and quantitative rtPCR in HIV adults.
  
  
  RESULTS
  The 102 adults with suspected CMV disease had lower LTCD4 count and higher HIV viral load than 77 patients without suspicion (p < 0.05). Antigenemia and PCR were positive in 47 (46.1%) and 37 (36.3%) adults with clinical suspicion and in 2 (2.6%) and 4 (5.2%) of 77 without suspicion. The sensitivity, specificity, positive and negative predictive value of antigenemia and RPCtr were 92%, 80%, 72% and 95% and 72%, 95%, 92% and 80%, respectively. The cutoff values were ≥ lcell (+) and ≥ 5.5 log10 copies/2 x 10(6) cells. CMV was isolated in 6/179 patients (3.4%), all symptomatic.
  
  
  CONCLUSIONS
  Positivity of antigenemia and rtPCR are similar for diagnosing CMV disease in Chilean HIV adults. AVR is inappropriate as a gold standard for its low performance.
authors.forename: Vivían
authors.initials: V
authors.lastname: Luchsinger
authors.forename: Patricia
authors.initials: P
authors.lastname: Vásquez
authors.forename: Matías
authors.initials: M
authors.lastname: Silva
authors.forename: M José
authors.initials: MJ
authors.lastname: Bruno
authors.forename: Izkia
authors.initials: I
authors.lastname: Siches
authors.forename: Javier
authors.initials: J
authors.lastname: Villarroel
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Garmendia
authors.forename: Carmen
authors.initials: C
authors.lastname: Larrañaga
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D000328:Adult; D000956:Antigens, Viral / Q000097:blood / Q000276:immunology*; D002677:Chile; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019562:Viral Load; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral