title: Multicenter Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Study for Identification of Clinically Relevant Nocardia spp.
abstract: This multicenter study analyzed Nocardia spp., including extraction, spectral acquisition, Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification, and score interpretation, using three Nocardia libraries, the Bruker, National Institutes of Health (NIH), and The Ohio State University (OSU) libraries, and compared the results obtained by each center. A standardized study protocol, 150 Nocardia isolates, and NIH and OSU Nocardia MALDI-TOF MS libraries were distributed to three centers. Following standardized culture, extraction, and MALDI-TOF MS analysis, isolates were identified using score cutoffs of ≥2.0 for species/species complex-level identification and ≥1.8 for genus-level identification. Isolates yielding a score of <2.0 underwent a single repeat extraction and analysis. The overall score range for all centers was 1.3 to 2.7 (average, 2.2 ± 0.3), with common species generally producing higher average scores than less common ones. Score categorization and isolate identification demonstrated 86% agreement between centers; 118 of 150 isolates were correctly identified to the species/species complex level by all centers. Nine strains (6.0%) were not identified by any center, and six (4.0%) of these were uncommon species with limited library representation. A categorical score discrepancy among centers occurred for 21 isolates (14.0%). There was an overall benefit of 21.2% from repeat extraction of low-scoring isolates and a center-dependent benefit for duplicate spotting (range, 2 to 8.7%). Finally, supplementation of the Bruker Nocardia MALDI-TOF MS library with both the OSU and NIH libraries increased the genus-level and species-level identification by 18.2% and 36.9%, respectively. Overall, this study demonstrates the ability of diverse clinical microbiology laboratories to utilize MALDI-TOF MS for the rapid identification of clinically relevant Nocardia spp. and to implement MALDI-TOF MS libraries developed by single laboratories across institutions.
authors.affiliation: National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA.
authors.forename: Sara J
authors.initials: SJ
authors.lastname: Blosser
authors.affiliation: National Institutes of Health, Critical Care Medicine Department, Bethesda, Maryland, USA.
authors.forename: Steven K
authors.initials: SK
authors.lastname: Drake
authors.affiliation: Cleveland Clinic, Pathology and Laboratory Medicine Institute, Cleveland, Ohio, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Andrasko
authors.affiliation: National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Henderson
authors.affiliation: The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, Ohio, USA.
authors.forename: Kamal
authors.initials: K
authors.lastname: Kamboj
authors.affiliation: National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA.
authors.forename: Stella
authors.initials: S
authors.lastname: Antonara
authors.affiliation: National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA.
authors.forename: Lilia
authors.initials: L
authors.lastname: Mijares
authors.affiliation: National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Conville
authors.affiliation: National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA.
authors.forename: Karen M
authors.initials: KM
authors.lastname: Frank
authors.affiliation: Cleveland Clinic, Pathology and Laboratory Medicine Institute, Cleveland, Ohio, USA.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Harrington
authors.affiliation: The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, Ohio, USA.
authors.forename: Joan-Miquel
authors.initials: JM
authors.lastname: Balada-Llasat
authors.affiliation: National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA azelazny@mail.nih.gov.
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Zelazny
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D009615:Nocardia / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000175:diagnosis* / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014481:United States