title: Simultaneous determination and pharmacokinetic study of four phenol compounds in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry after oral administration of Echinacea purpurea extract.
abstract: A rapid and sensitive assay based on ultra-high performance liquid chromatography with electrospray ionization tandem mass spectrometry was established and validated for the simultaneous determination of cichoric acid, chlorogenic acid, quinic acid, and caffeic acid in rat plasma after oral administration of Echinacea purpurea extract using butylparaben as the internal standard. Samples were pretreated by liquid-liquid extraction with ethyl acetate. The separations for analytes were performed on an ACQUITY UPLC HSS C18 column (1.8 μm 2.1 × 100 mm) using a gradient elution program with acetonitrile/10 mM ammonium acetate (pH 5.6) at a flow rate of 0.3 mL/min. The analytes were detected in multiple reaction monitoring mode with negative electrospray ionization. The lower limit of quantification of each analyte was not higher than 10.85 ng/mL. The relative standard deviation of the intraday and interday precisions was less than 14.69%. The relative errors of accuracies were in the range of -13.80 to 14.91%. The mean recoveries for extraction recovery and matrix effect were higher than 80.79 and 89.98%, respectively. The method validation results demonstrated that the proposed method was sensitive, specific, and reliable, which was successfully applied to the pharmacokinetic study of four components after oral administration of Echinacea purpurea extract.
authors.affiliation: College of Pharmacy, Harbin Medical University, Nangang District, Harbin, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Gan
authors.affiliation: College of Pharmacy, Harbin Medical University, Nangang District, Harbin, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Harbin Medical University, Nangang District, Harbin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Pharmacy, Harbin Medical University, Nangang District, Harbin, China.
authors.forename: Liqian
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Harbin Medical University, Nangang District, Harbin, China.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Pharmacy, Harbin Medical University, Nangang District, Harbin, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.affiliation: College of Pharmacy, Harbin Medical University, Nangang District, Harbin, China.
authors.forename: Chunjuan
authors.initials: C
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002109:Caffeic Acids / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D002726:Chlorogenic Acid / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D020900:Echinacea / Q000737:chemistry*; D008297:Male; D008968:Molecular Conformation; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D011801:Quinic Acid / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013386:Succinates / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry
chemical_list: D002109:Caffeic Acids; D010936:Plant Extracts; D013386:Succinates; D011801:Quinic Acid; D002726:Chlorogenic Acid; C100435:chicoric acid; C040048:caffeic acid
keywords: Echinacea purpurea; Herbal medicines; Pharmacokinetics; Rat plasma; Ultra-high performance liquid chromatography