title: [Expression of Tim-3 on peripheral blood CD4+ T cells and its correlation with liver function damage parameters in advanced schistosomiasis patients].
abstract: OBJECTIVE
  To explore the expression levels of T cell immunoglobulin and mucin domain protein 3 (Tim-3) on peripheral blood CD4+ T cells and its correlation with liver function damage parameters in advanced schistosomiasis patients.
  
  
  METHODS
  Totally 28 advanced schistosomiasis patients were selected as study subjects, and 20 chronic schistosomiasis patients and 30 healthy persons were selected as controls. The expression levels of Tim-3 on CD4+ T cell were detected by flow cytometry, and the serum IFN-γ and IL-4 levels were detected by ELISA. Hitachi 7600 biochemical analyzer was used to analyze the liver function parameters (ALT, γ-GT and TBIL).
  
  
  RESULTS
  There was a significant difference in Tim-3 expression among the three groups (F = 4.578,P < 0.05). Tim-3 expression level in advanced schistosomiasis patients was (8.33 ± 2.28)%, which was significantly higher than that of the healthy control group (6.57 ± 1.99)% (t = 3.015, P < 0.01). The Spearman nonparametric correlation analysis showed that the Tim-3 expression level on CD4+ T cells in advanced schistosomiasis patients was positively correlated with the serum ALT(r, = 0.746, P < 0.01), γ-GT(r, = 0.656, P < 0.01) and IL-4(r, = 0.672, P < 0.01) levels, but negatively correlated with the serum IFN-γ levels (r(s) = -0.404, P < 0.05).
  
  
  CONCLUSIONS
  The expression of Tim-3 on peripheral CD4' T cells is increased in advanced schistosomiasis patients, which may regulate the function of CD4' T cells and then be involved in the liver damage process of advanced schistosomiasis.
authors.forename: Qi-wen
authors.initials: QW
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhu
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Fu
authors.forename: Jin-sun
authors.initials: JS
authors.lastname: Yang
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Cao
mesh_terms: D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D000072597:Hepatitis A Virus Cellular Receptor 2; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008111:Liver Function Tests; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D012552:Schistosomiasis / Q000097:blood* / Q000473:pathology; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D008565:Membrane Proteins; D015847:Interleukin-4; D007371:Interferon-gamma; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase