title: Role of Hematopoietic Stem Cells in Inflammation of the Pancreas during Diabetes Mellitus.
abstract: The model of streptozotocin-induced diabetes mellitus in C57Bl/6 mice was employed to study the role of precursors of insulin-producing β-cells, hematopoietic stem cells, and progenitor hematopoietic cells in inflammation. In addition to provoking hyperglycemia, streptozotocin elevated serum levels of IL-1β and hyaluronic acid, induced edema in the pancreatic insular tissue and its infiltration by inflammatory cells (neutrophils, lymphocytes, and macrophages) and fibroblasts. Inflammation in pancreatic islets was accompanied by necrotic processes and decreasing counts of multipotent progenitor β-cells (CD45(-), TER119(-), c-kit-1(-), and Flk-1(-)), oligopotent progenitor β-cells (CD45(-), TER119(-), CD133(+), and CD49f(low)), and insulinproducing β-cells (Pdx1(+)). Pancreatic infl ammation was preceded by elevation of the number of short-term hematopoietic stem cells (Lin-Sca-1(+)c-kit(+)CD34(+)) relative to long-term cells (Lin(-)Sca-1(+)c-kit(+)CD34(-)) in the bone marrow as well as recruitment of hematopoietic stem and progenitor cells into circulation. Transplantation of bone marrow hematopoietic stem and progenitor cells from diabetic C57Bl/6 donor mice to recipient CBA mice with 5-fluorouracilinduced leukopenia accelerated regeneration of granulocytopoiesis in recipient mice.
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regene rative Medicine, Tomsk, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Dygai
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regene rative Medicine, Tomsk, Russia. eskurihin@inbox.ru.
authors.forename: E G
authors.initials: EG
authors.lastname: Skurikhin
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regene rative Medicine, Tomsk, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Pershina
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regene rative Medicine, Tomsk, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Ermakova
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regene rative Medicine, Tomsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Krupin
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regene rative Medicine, Tomsk, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Ermolaeva
authors.affiliation: Tomsk Research Institute of Oncology, Tomsk, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Stakheeva
authors.affiliation: Tomsk Research Institute of Oncology, Tomsk, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Choinzonov
authors.affiliation: Tomsk Research Institute of Oncology, Tomsk, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Goldberg
authors.affiliation: E. D. Goldberg Research Institute of Pharmacology and Regene rative Medicine, Tomsk, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Reikhart
authors.affiliation: A. M. Nikiforov All-Russian Center of Emergency and Radiation Medicine, the Ministry of Emergency Situations, St. Petersburg, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Ellinidi
authors.affiliation: A. M. Nikiforov All-Russian Center of Emergency and Radiation Medicine, the Ministry of Emergency Situations, St. Petersburg, Russia.
authors.forename: V Yu
authors.initials: VY
authors.lastname: Kravtsov
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000628:therapy*; D005472:Fluorouracil; D006098:Granulocytes / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006820:Hyaluronic Acid / Q000097:blood; D006943:Hyperglycemia / Q000139:chemically induced / Q000628:therapy*; D007249:Inflammation / Q000628:therapy; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000473:pathology; D053583:Interleukin-1beta / Q000097:blood; D007970:Leukopenia / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013311:Streptozocin
chemical_list: C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D013311:Streptozocin; D006820:Hyaluronic Acid; D005472:Fluorouracil
keywords: IL-1β; diabetic inflammation; hematopoietic stem cells; hyaluronic acid; multipotent precursors of β-cells