title: Ingramon, a Peptide Inhibitor of MCP-1 Chemokine, Reduces Migration of Blood Monocytes Stimulated by Glioma-Conditioned Medium.
abstract: Malignant gliomas are most common and fatal primary brain tumors. In addition to neoplastic cells, the tumor tissue contains microglial cells and monocyte-derived macrophages. It is an established fact that monocyte recruiting promotes the tumor growth and dissemination. Monocyte chemotactic protein-1 (MCP-1) is the major attractant for monocytes. We have previously synthesized an MCP-1 antagonist ingramon, a synthetic peptide fragment (65-76) of this chemokine. In the present study, we demonstrated that glioma-conditioned medium contains MCP-1 and stimulates migration of blood monocytes. Ingramon inhibited the effect of glioma-conditioned medium on monocyte migration.
authors.affiliation: Russian Cardiology Research-and-Development Centre, |Ministry of Health of the Russian Federation, Moscow, Russia. tlkrasnikova@gmail.com.
authors.forename: T L
authors.initials: TL
authors.lastname: Krasnikova
authors.affiliation: Russian Cardiology Research-and-Development Centre, |Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Arefieva
authors.affiliation: Russian Cardiology Research-and-Development Centre, |Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Pylaeva
authors.affiliation: Russian Cardiology Research-and-Development Centre, |Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Sidorova
mesh_terms: D045744:Cell Line, Tumor; D018932:Chemokine CCL2 / Q000037:antagonists & inhibitors*; D002633:Chemotaxis / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D017077:Culture Media, Conditioned; D010446:Peptide Fragments; C542772:ingramon
keywords: cell migration; glioma; monocyte chemotactic protein-1 (MCP-1); monocytes; peptide inhibitor