title: Self-patterning of adipose-derived mesenchymal stem cells and chondrocytes cocultured on hyaluronan-grafted chitosan surface.
abstract: The articular cartilage, once injured, has a limited capacity for intrinsic repair. Preparation of functionally biocartilage substitutes in vitro for cartilage repair is an attractive concept with the recent advances in tissue engineering. In this study, adipose-derived adult stem cells (ADAS) and chondrocytes (Ch) were cocultured in different population ratios on the surface of hyaluronan-grafted chitosan (CS-HA) membranes. The two types of cells could self-assemble into cospheroids with different morphologies. In particular, when ADAS and Ch were cocultured at an initial ratio of 7:3 on CS-HA surface, the expression of chondrogenic markers was upregulated, leading to preferred chondrogenesis of the cospheroids. Therefore, using the ADAS/Ch 7:3 cospheroids derived on CS-HA surface instead of using only a single type of cells may be favorable for future therapeutic applications.
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Hsi-Yi
authors.initials: HY
authors.lastname: Yeh
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Fu-Yu
authors.initials: FY
authors.lastname: Hsieh
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Shan-hui
authors.initials: SH
authors.lastname: Hsu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D019902:Chondrocytes / Q000502:physiology*; D018920:Coculture Techniques; D006820:Hyaluronic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D018874:Spheroids, Cellular; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D006820:Hyaluronic Acid; D048271:Chitosan