title: [The expression and clinopathological significance of miR-130b in human hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the expression of miR-130b in human hepatocellular carcinoma (HCC) and its correlations with clinical-pathological features.
  
  
  METHODS
  Real-time quantitative PCR (qRT-PCR) was applied to detect the expression of miR-130b in HCC tissues (n=86), matched normal tumor-adjacent tissues and 5 HCC cell lines (LO2 human normal hepatocytes, HepG2, Hep3B, SMMC-7721, Hu7 cells). The expressions of peroxisome proliferator-activated receptor γ (PPARγ), E-cadherin and vimentin were measured by immunohistochemistry. miR-130b inhibitor synthesized artificially was transfected into SMMC-7721 cells in vitro. Cell invasion was analyzed by Transwell(TM) assay. The expressions of PPARγ, E-cardhern and vimentin in SMMC-7721 cells after transfected with miR-130b inhibitor and PPARγ siRNA were detected by qRT-PCR and Western blotting.
  
  
  RESULTS
  The expression of miR-130b mRNA in HCC tissues was significantly higher than that in matched normal tumor-adjacent tissues. Clinical analysis indicated that high expression of miR-130b was prominently correlated with venous infiltration, high Edmondson-Steiner grading and advanced tumor node metastasis (TNM) stage. Elevated miR-130b expression was observed in all HCC cell lines (HepG2, SMMC-7721, Huh7 and Hep3B) as compared with that in LO2 nontransformed hepatic cell line. Furthermore, there was an inverse correlation between miR-130b and E-cadherin as well as between miR-130b and PPARγ, and a positive correlation between miR-130b and vimentin was demonstrated in HCC tissues. miR-130b inhibitor could significantly increase the expression of PPARγ and E-cadherin, but decrease the expression of vimentin in SMMC-7721 cells, meanwhile it suppressed the migration and invasion of SMMC-7721 cells. In addition, the down-regulation of PPARγ expression by PPARγ siRNA partially abrogated the above effect of miR-130b on HCC cells.
  
  
  CONCLUSIONS
  The expression of miR-130b in HCC tissues is significantly higher than that in tumor-adjacent tissues. The increased expression of miR-130b is related with the malignant manifestations of HCC. miR-130b may promote HCC cell invasion by inhibiting PPARγ expression and inducing epithelial-mesenchymal transition.
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Qiuran
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Wenwei
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Emergency, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Meiqi
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. *Corresponding authors, E-mail: liuxindongdong@sina.com.
authors.forename: Qingguang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Zhejiang Provincial People's Hospital, Hangzhou 310014, China. *Corresponding authors, E-mail: liuqingguang@vip.sina.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; C554019:MIRN130 microRNA, human; D035683:MicroRNAs; D047495:PPAR gamma; D014746:Vimentin