title: [Time course study of growth hormone releasing peptide-6-induced c-fos expression in neurons of feeding-related nuclei of hypothalamus].
abstract: The present study was aimed to explore the effects of intraperitoneal injection of growth hormone releasing peptide-6 (GHRP-6), a ghrelin receptor agonist, on food intake and neuronal activity of feeding-related nuclei in the hypothalamus of NMRI mice. Accumulated amount of food intake was measured, and total number of c-fos immunoreactive neurons in arcuate nucleus (ARC), paraventricular nucleus (PVN) and supraoptic nucleus (SON) was counted by immunohistochemistry at 1, 3 and 6 h after the GHRP-6 injection. The results showed that GHRP-6 significantly increased the amount of food intake with a peak at 3 h after the GHRP-6 injection. Meanwhile, GHRP-6 could promote c-fos expression in the ARC and PVN independent of food intake, and the total number of c-fos immunoreactive neurons was peaked at 1 h after injection and then decreased gradually. These results suggest that GHRP-6 may increase food intake in time-dependent manner, which is associated with up-regulations of c-fos protein expression in the ARC and PVN.
authors.affiliation: Department of Special Medicine, Qingdao Medical College of Qingdao University, Qingdao 266071, China.
authors.forename: Yan-Juan
authors.initials: YJ
authors.lastname: Yu
authors.affiliation: Department of Special Medicine, Qingdao Medical College of Qingdao University, Qingdao 266071, China.|Department of Physiology, Qingdao Medical College of Qingdao University, Qingdao 266071, China. dongjing6@hotmail.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Physiology, Qingdao Medical College of Qingdao University, Qingdao 266071, China.
authors.forename: Zheng-Yao
authors.initials: ZY
authors.lastname: Jiang
authors.affiliation: Center for Gastroenterological Research, KU Leuven, Leuven, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Depoortere
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus; D004435:Eating; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009474:Neurons; D009842:Oligopeptides; D010286:Paraventricular Hypothalamic Nucleus; D016760:Proto-Oncogene Proteins c-fos; D054440:Receptors, Ghrelin; D013495:Supraoptic Nucleus
chemical_list: D009842:Oligopeptides; D016760:Proto-Oncogene Proteins c-fos; D054440:Receptors, Ghrelin; C041048:growth hormone releasing hexapeptide