title: Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol-yne click chemistry for the separation of basic compounds.
abstract: A novel silica-based stationary phase with branched octadecyl groups was prepared by the sequential employment of the Michael addition reaction and photoinduced thiol-yne click chemistry with 3-aminopropyl-functionalized silica microspheres as the initial material. The resulting stationary phase denoted as SiO2 -N(C18)4 was characterized by elemental analysis, FTIR spectroscopy and Raman spectroscopy, demonstrating the existence of branched octadecyl groups in silica microspheres. The separations of benzene homologous compounds, acid compounds and amine analogues were conducted, demonstrating mixed-mode separation mechanism on SiO2 -N(C18)4 . Baseline separation of basic drugs mixture was acquired with the mobile phase of acetonitrile/H2 O (5%, v/v). SiO2 -N(C18)4 was further applied to separate Corydalis yanhusuo Wang water extracts, and more baseline separation peaks were obtained for SiO2 -N(C18)4 than those on Atlantis dC18 column. It can be expected that this new silica-based stationary phase will exhibit great potential in the analysis of basic compounds.
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China.|University of Chinese Academy of Sciences, Beijing, China.|State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Huang
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Ou
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China.|University of Chinese Academy of Sciences, Beijing, China.|State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Ji
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wan
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China.
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
keywords: Basic compounds; High-performance liquid chromatography; Michael addition; Stationary phases; Thiol-yne click chemistry