title: Cellular uptake of PLA nanoparticles studied by light and electron microscopy: synthesis, characterization and biocompatibility studies using an iridium(III) complex as correlative label.
abstract: We present the synthesis of polylactide by ring-opening polymerization using a luminescent iridium(III) complex acting as initiator. The polymer was formulated into nanoparticles, which were taken up by HEK-293 cells. We could show that the particles provided an appropriate contrast in both superresolution fluorescence and electron microscopy, and, moreover, are non-toxic, in contrast to the free iridium complex.
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany. s.hoeppener@uni-jena.de ulrich.schubert@uni-jena.de and Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, D-07743 Jena, Germany and Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, D-07743 Jena, Germany. rainer.heintzmann@uni-jena.de and Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, D-07745 Jena, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Reifarth
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany. s.hoeppener@uni-jena.de ulrich.schubert@uni-jena.de and Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, D-07743 Jena, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Pretzel
authors.affiliation: Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, D-07743 Jena, Germany and Institute of Pharmacy, Department of Pharmaceutical Technology, Friedrich Schiller University Jena, Otto-Schott-Str. 41, D-07745 Jena, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Schubert
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany. s.hoeppener@uni-jena.de ulrich.schubert@uni-jena.de and Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, D-07743 Jena, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Weber
authors.affiliation: Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, D-07743 Jena, Germany. rainer.heintzmann@uni-jena.de and Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, D-07745 Jena, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Heintzmann
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany. s.hoeppener@uni-jena.de ulrich.schubert@uni-jena.de and Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, D-07743 Jena, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Hoeppener
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, D-07743 Jena, Germany. s.hoeppener@uni-jena.de ulrich.schubert@uni-jena.de and Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, D-07743 Jena, Germany.
authors.forename: Ulrich S
authors.initials: US
authors.lastname: Schubert
mesh_terms: D001672:Biocompatible Materials; D057809:HEK293 Cells; D006801:Humans; D007495:Iridium / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D008854:Microscopy, Electron / Q000379:methods*; D053758:Nanoparticles; D011091:Polyesters; D011108:Polymers / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; D007495:Iridium; C033616:poly(lactide)