title: [Embryo development in two single-step media: Analysis of 2059 sibling oocytes].
abstract: OBJECTIVE
  The aim of this study was to compare embryo development cultured in two single-step media commercially available: Fert/Sage One Step® (Origio) and Continuous Single Culture® (CSC) (Irvine Scientific).
  
  
  METHODS
  A prospective auto-controlled study of sibling oocytes from women undergoing conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) was performed in our center from February to June 2015. After fertilization, for every patient, half of oocytes were cultured in the single-step Fert/Sage One Step® (serie SAGE) and the other half in the single-step CSC®(serie CSC). Fertilization and embryo morphology rates were assessed by day 1 to day 5-6 if needed. Embryo presenting<20% of fragmentation and 4 cells at day 2, 8 cells at day 3 were qualified as "top quality". Embryo with<20% of fragmentation and 3-5 cells at day 2, 6-10 cells at day 3 were qualified as "good quality". Blastocyst B3, B4, B5 with A or B inner cell mass and A or B trophectoderm were qualified as "good quality". Transferred or frozen embryos were qualified as useful embryos.
  
  
  RESULTS
  Sixty-two attempts of IVF and 133 of ICSI were analyzed, corresponding to 2059 inseminated or micro-injected oocytes. Fertilization rate were not different between the 2 series, respectively SAGE vs CSC (IVF: 73.4% vs 68.3% [P=0.49]; ICSI: 58.9% vs 63.8% [P=0.12]). No difference was found for embryo morphology, respectively SAGE vs CSC, at day 2 (top quality embryo at day 2 IVF: 34.4% vs 33% [P=0.98]; ICSI: 42.4% vs 44.9% [P=0.37]; and good quality embryo at day 2 IVF: 44% vs 50.2% [P=0.07]; ICSI: 64% vs 71% [P=0.35]); no difference at day 3 (top quality embryo at day 3 IVF: 19.4% vs 21.3% [P=0.61]; ICSI: 28.7% vs 27.4% [P=0.54]; and good quality embryo at day 3 IVF: 40.4% vs 50.2% [P=0.91]; ICSI: 51% vs 47.6% [P=0.47]). Blastocyst development rate were not different, respectively SAGE vs CSC (IVF: 39.9% vs 41.5% [P=0.63] with 42.9% vs 42.2% of good quality blastocyst [P=0.70]; ICSI: 41.1% vs 37.8% [P=0.18] with 32.9% vs 40.8% of good quality blastocyst [P=0.13]). No difference was found in the useful embryo rate in the 2 series SAGE vs CSC (IVF: 52.8% vs 55.2% [P=0.83]; ICSI: 62.4% vs 61.7% [P=0.70]).
  
  
  CONCLUSIONS
  Embryo development and rate of useful embryos, transferred or frozen, were not different according to the embryo culture in single-step media Fert/Sage One Step® vs single-step Continuous Single Culture®.
authors.affiliation: Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France. Electronic address: marine.durand@aphp.fr.
authors.forename: M
authors.initials: M
authors.lastname: Durand
authors.affiliation: Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France.
authors.forename: N
authors.initials: N
authors.lastname: Sermondade
authors.affiliation: Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France.
authors.forename: C
authors.initials: C
authors.lastname: Herbemont
authors.affiliation: Service de médecine de la reproduction, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, 93140 Bondy, France.
authors.forename: J
authors.initials: J
authors.lastname: Benard
authors.affiliation: Service de médecine de la reproduction, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, 93140 Bondy, France.
authors.forename: H
authors.initials: H
authors.lastname: Gronier
authors.affiliation: Service de médecine de la reproduction, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, 93140 Bondy, France.
authors.forename: J
authors.initials: J
authors.lastname: Boujenah
authors.affiliation: Service de médecine de la reproduction, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, 93140 Bondy, France.
authors.forename: I
authors.initials: I
authors.lastname: Cédrin-Durnerin
authors.affiliation: Service de médecine de la reproduction, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, 93140 Bondy, France.
authors.forename: C
authors.initials: C
authors.lastname: Poncelet
authors.affiliation: Service de médecine de la reproduction, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, 93140 Bondy, France.
authors.forename: M
authors.initials: M
authors.lastname: Grynberg
authors.affiliation: Service d'histologie-embryologie-cytogénétique-CECOS, centre hospitalo-universitaire Jean-Verdier, Assistance publique-Hôpitaux de Paris, avenue du 14-Juillet, 93140 Bondy, France.
authors.forename: C
authors.initials: C
authors.lastname: Sifer
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000502:physiology; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes / Q000502:physiology*; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D003470:Culture Media
keywords: Embryo culture media; FIV; ICSI; IVF; Milieu de culture embryonnaire; Milieu global; Single-step media