title: Immunological evidence of Zika virus transmission in Thailand.
abstract: OBJECTIVE
  To identify immunological evidence of Zika virus transmission in Thailand.
  
  
  METHODS
  To undertake a preliminary serosurvey of possible exposure to Zika virus, 21 serum samples from cohort of acute undifferentiated fever patients were examined for immunoreactivity to Zika, Dengue, Japanese encephalitis and Chikungunya envelope antigens by Western blot analysis.
  
  
  RESULTS
  Twenty of the 21 serum samples showed immunoreactivity to at least one of the antigens, with seven samples showing immunoreactivity to all antigens. Of particular note, two serum samples showed immunoreactivity only to Zika envelope antigen, with no immunoreactivity to other envelope antigens.
  
  
  CONCLUSIONS
  This study presents the first evidence of Zika virus transmission in Thailand, although as yet the relationship between transmission and possible cases of Zika fever in Thailand requires further investigation.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Nitwara
authors.initials: N
authors.lastname: Wikan
authors.affiliation: Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Yupin
authors.initials: Y
authors.lastname: Suputtamongkol
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.
authors.forename: Sutee
authors.initials: S
authors.lastname: Yoksan
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand. Electronic address: duncan_r_smith@hotmail.com.
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
authors.affiliation: Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Auewarakul
keywords: Emerging infectious diseases; Serosurvey; Thailand; Zika virus