title: S100a8/NF-κB signal pathway is involved in the 800-nm diode laser-induced skin collagen remodeling.
abstract: The 800-nm diode laser is widely used for hair removal and also promotes collagen synthesis, but the molecular mechanism by which dermis responses to the thermal damage induced by the 800-nm diode laser is still unclear. Ten 2-month-old mice were irradiated with the 800-nm diode laser at 20, 40, and 60 J/cm(2), respectively. Skin samples were taken for PCR, Western blot analysis, and histological study at day 3 or 30 after laser irradiation. The expression of S100a8 and its two receptors (advanced glycosylation end product-specific receptor, RAGE and toll-like receptor 4, TRL4) was upregulated at day 3 after laser treatments. P-p65 levels were also elevated, causing the increase of cytokine (tumor necrosis factor, TNF-α and interleukin 6, IL-6) and MMPs (MMP1a, MMP9). At day 30, PCR and Western blot analysis showed significant increase of type I and III procollagen in the dermis treated with laser. Importantly, skin structure was markedly improved in the laser-irradiated skin compared with the control. Thus, it seemed that S100a8 upregulation triggered NF-κB signal pathway through RAGE and TLR4, responding to laser-induced dermis wound healing. The involvement of the NF-κB pathway in MMP gene transcription promoted the turnover of collagen in the skin, accelerating new collagen synthesis.
authors.affiliation: Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Dermatology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China.
authors.forename: Minggai
authors.initials: M
authors.lastname: Ge
authors.affiliation: Department of Dermatology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Qin
authors.affiliation: Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
authors.forename: Pingya
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai, 200241, China. dangyongyan@gmail.com.
authors.forename: Yongyan
authors.initials: Y
authors.lastname: Dang
authors.affiliation: Department of Dermatology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. gujun79@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Dermatology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China. xyye@bio.ecnu.edu.cn.
authors.forename: Xiyun
authors.initials: X
authors.lastname: Ye
mesh_terms: D000818:Animals; D040501:Calgranulin A / Q000378:metabolism; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D003357:Cosmetic Techniques; D005260:Female; D015870:Gene Expression / Q000528:radiation effects; D015850:Interleukin-6 / Q000378:metabolism; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D015595:Skin Aging / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D040501:Calgranulin A; D015850:Interleukin-6; D016328:NF-kappa B; C086145:S100a8 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse
keywords: 800-nm diode laser; NF-κB; S100a8; Skin collagen remodeling