title: Rational design of hybridization chain reaction monomers for robust signal amplification.
abstract: We established four-point guidelines for the sequence design of hairpin monomers in hybridization chain reaction (HCR). This enabled greater flexibility to customize specific hairpin sequences for use with the readout platform of interest. Using shorter hairpin stem length, a one-pot signal amplification system was demonstrated by incorporating distance-sensitive Förster resonance energy transfer (FRET) readout.
authors.affiliation: Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585. cheyly@nus.edu.sg.
authors.forename: Yan Shan
authors.initials: YS
authors.lastname: Ang
authors.affiliation: Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585. cheyly@nus.edu.sg.
authors.forename: Lin-Yue Lanry
authors.initials: LY
authors.lastname: Yung