title: Effect of sildenafil on intestinal adaptation parameters in a rat model of short bowel syndrome.
abstract: OBJECTIVE
  We aimed to evaluate the effect of sildenafil on the intestinal adaptation in short bowel syndrome (SBS).
  
  
  METHODS
  Forty-eight male Wistar-albino rats (weight, 231-390 g) were randomly divided into four groups with 12 rats in each. Group TA had only ileal transection+anastomosis, Group TA+S was given sildenafil after ileal transection+anastomosis, Group RA had a resection of 75% of the small bowel+anastomosis, Group RA+S was given sildenafil after small bowel resection+anastomosis. Sildenafil was injected subcutaneously at 60 mg/kg/day dose throughout 3-21 days postoperatively. Bowel and mucosal weights, villus height, crypt depth, DNA and protein concentrations were determined.
  
  
  RESULTS
  Jejunal bowel weight was lower in TA and TA+S groups than RA and RA+S groups (p < 0.05). RA+S group had higher ileal and jejunal mucosal weights than RA and TA+S groups (p < 0.05). Villus height was highest in RA+S group both in ileum and jejunum (466.1 ± 38.6 μm and 648.1 ± 65.7 μm, respectively). Jejunal crypt depth was highest in RA+S group (255.1 ± 21.9 μm) compared to other groups (p < 0.05). There was no significant difference in ileal and jejunal protein concentration between TA and TA+S groups and in ileal protein concentration between RA ve RA+S groups (p > 0.05). Ileal DNA concentration was higher in TA+S group, and jejunal DNA concentration was higher in RA and RA+S groups than TA and TA+S groups (p < 0.05).
  
  
  CONCLUSIONS
  Sildenafil has a positive effect on intestinal adaptation parameters, particularly in jejunum in a rat SBS model. Thus, its role in the treatment of SBS should be further investigated with clinical studies.
authors.affiliation: Department of Surgery, Sinop State Hospital, Sinop, Turkey. tbilecik@yahoo.com.
authors.forename: T
authors.initials: T
authors.lastname: Bilecik
authors.forename: C
authors.initials: C
authors.lastname: Ensari
authors.forename: B
authors.initials: B
authors.lastname: Mayir
authors.forename: T
authors.initials: T
authors.lastname: Cakir
authors.forename: E
authors.initials: E
authors.lastname: Minareci
authors.forename: U
authors.initials: U
authors.lastname: Arslan
authors.forename: T
authors.initials: T
authors.lastname: Oruc
authors.forename: R
authors.initials: R
authors.lastname: Eryilmaz
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D004195:Disease Models, Animal; D007082:Ileum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012778:Short Bowel Syndrome / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D000068677:Sildenafil Citrate / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D004247:DNA; D000068677:Sildenafil Citrate