title: A novel electrospinning approach to fabricate high strength aqueous silk fibroin nanofibers.
abstract: The present paper describes a rapid method of producing concentrated aqueous regenerated Bombyx mori silk fibroin (RSF) solution by applying mild shearing under forced dehumidified air and generation of electrospun SF nanofibers from concentrated solution with high mechanical strength using free liquid surface electrospinning machine. The shear induced concentrating mechanism favoured the electrospinning process by enhancing the viscosity (>2.43Pas as onset for electrospinning) and decreasing the surface tension of the solution (40.1-37.7mN/m). Shearing reduced the β-turns and random coil molecular conformation and thereby, intensified the β-sheet content from 16.9% to 34% which is the minimum content needed to commence RSF nanofibers formation. Subsequently, electrospun nanofibrous mats were produced from different batches of concentrated SF solutions (15-21wt%). Among the concentrated RSF, 17wt% RSF solution was the most favourable concentration producing electrospun nanofibrous mat having lowest average fiber diameters of 183±55nm and good tensile strength. The mechanical strength of the nanofibrous sheet was further improved by cross-linking with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide (EDC+NHS) which might be due to enhancement of β-sheet content. These nanofibers exhibited 17.57±1.13MPa ultimate tensile strength, 12.48±1.46% tensile strain at break and 37.7% increase in root mean square surface roughness which is favourable feature for cell adhesion and neo-tissue formation.
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: B N
authors.initials: BN
authors.lastname: Singh
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: N N
authors.initials: NN
authors.lastname: Panda
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India. Electronic address: kpr@nitrkl.ac.in.
authors.forename: K
authors.initials: K
authors.lastname: Pramanik
mesh_terms: D004560:Electricity; D005349:Fibroins / Q000737:chemistry*; D055595:Mechanical Phenomena; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011487:Protein Conformation; D012212:Rheology; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D012997:Solvents; D014867:Water; D005349:Fibroins
keywords: Conformational transition; Electrospinning; Regenerated silk fibroin; β-Sheet content