title: Content of Soluble Factors and Characteristics of Stromal Vascular Fraction Cells in Lipoaspirates from Different Subcutaneous Adipose Tissue Depots.
abstract: BACKGROUND
  Although fat grafting has emerged as a major force in plastic, reconstructive, and aesthetic surgery, some questions regarding its reliability and regenerative potential remain unanswered.
  
  
  OBJECTIVE
  The authors examined the influence of three anatomic areas on various lipoaspirate properties to identify the most appropriate harvest site for fat-grafting procedures.
  
  
  METHODS
  Lipoaspirates from 25 healthy patients were harvested from the abdomen, inner thigh, and knee. The authors measured the content of soluble factors in the lipoaspirate followed by the assessment of the yield, adipogenic differentiation, proliferation of stromal vascular fraction (SVF) cells, and the percentage of adipose-derived stem cells (ASC) in the SVF. The results also were correlated with the age and body mass index of the donors.
  
  
  RESULTS
  Lipoaspirates from the abdomen showed significantly higher concentrations of matrix metalloproteinase (MMP)-9 compared with the knee. The content of basic fibroblast growth factor (b-FGF), platelet-derived growth factor (PDGF)-BB, and insulin-like growth factor (IGF)-1 tended to be highest in the abdomen but did not reach statistical significance. Vascular endothelial growth factor (VEGF)-A and bFGF-2 contents both correlated negatively with age in lipoaspirates from at least two different anatomic areas.
  
  
  CONCLUSIONS
  The authors' results indicate that the abdomen may be a slight favorite over the inner thigh and knee because of its richer content of soluble factors. However, because only the difference of MMP-9 content actually reached statistical significance and because no differences in SVF characteristics were observed, a decision primarily based on other criteria appears to be justifiable.
authors.affiliation: From the Department of Plastic and Reconstructive Surgery, Hand Surgery - Burn Center, Medical Faculty, RWTH Aachen University, Aachen, Germany.
authors.forename: Justinas
authors.initials: J
authors.lastname: Grasys
authors.affiliation: From the Department of Plastic and Reconstructive Surgery, Hand Surgery - Burn Center, Medical Faculty, RWTH Aachen University, Aachen, Germany.
authors.forename: Bong-Sung
authors.initials: BS
authors.lastname: Kim
authors.affiliation: From the Department of Plastic and Reconstructive Surgery, Hand Surgery - Burn Center, Medical Faculty, RWTH Aachen University, Aachen, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Pallua
mesh_terms: D050153:Abdominal Fat / Q000166:cytology / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007717:Knee; D015187:Lipectomy; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D011446:Prospective Studies; D015203:Reproducibility of Results; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology; D050151:Subcutaneous Fat / Q000166:cytology* / Q000378:metabolism / Q000601:surgery*; D013848:Thigh; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D055815:Young Adult
chemical_list: D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I; D020780:Matrix Metalloproteinase 9