title: [Preparation of rabbit polyclonal antibody against mouse ADAMTS2].
abstract: OBJECTIVE
  To obtain recombinant mouse collagenase ADAMTS2 (ADAM metallopeptidase with thrombospondin type 1 motif, 2) C terminal (1109-1213), and prepare the corresponding rabbit anti-ADAMTS2 polyclonal antibodies.
  
  
  METHODS
  The recombinant expression plasmid pGEX-6p-1-ADAMTS2 (1109-1213) was transformed into E.coli. The target protein was induced by IPTG and identified by mass spectrometry following affinity purification. The expressed and purified ADAMTS2 (1109-1213) protein was used to immunize New Zealand rabbits to prepare anti-ADAMTS2 polyclonal antibodies. The antibody titers were detected by ELISA and the antibody specificity by Western blotting.
  
  
  RESULTS
  The protein ADAMTS2 (1109-1213) was expressed in E.coli after IPTG induction, and with the purified protein, we prepared antiserum in the immunized rabbits. The titer of the antiserum reached over 1:160 000. The antiserum showed a good specificity.
  
  
  CONCLUSIONS
  The high titer and specific rabbit anti-ADAMTS2 antibody has been prepared successfully.
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
authors.forename: Shiying
authors.initials: S
authors.lastname: Miao
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100005, China. *Corresponding author, E-mail: wang.linfang@imicams.ac.cn.
authors.forename: Linfang
authors.initials: L
authors.lastname: Wang
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000071096:ADAMTS Proteins; D000071121:ADAMTS4 Protein; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D008297:Male; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011348:Procollagen N-Endopeptidase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D007106:Immune Sera; D010446:Peptide Fragments; D011994:Recombinant Proteins; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; C000609308:Adamts2 protein, mouse; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein