title: Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization.
abstract: Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; School of life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Choi
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea.
authors.forename: Sung Nam
authors.initials: SN
authors.lastname: Kang
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; School of life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Seong Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Samy
authors.initials: S
authors.lastname: Gobaa
authors.affiliation: Department of Electronic Engineering & Institute of Gachon Fusion Technology, Gachon University, 1342 Seongnamdaero, Seongnam-si, Gyeonggi 146-701, Republic of Korea.
authors.forename: Bang Ju
authors.initials: BJ
authors.lastname: Park
authors.affiliation: School of life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Ik Hwan
authors.initials: IH
authors.lastname: Kim
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea. Electronic address: ykjoung@kist.re.kr.
authors.forename: Yoon Ki
authors.initials: YK
authors.lastname: Joung
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Biomedical Engineering, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea. Electronic address: dkh@kist.re.kr.
authors.forename: Dong Keun
authors.initials: DK
authors.lastname: Han
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D065546:Drug Liberation; D054855:Drug-Eluting Stents; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D017228:Hepatocyte Growth Factor / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013450:Sulfones / Q000737:chemistry; D013499:Surface Properties; D042461:Vascular Endothelial Growth Factor A / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D013450:Sulfones; D042461:Vascular Endothelial Growth Factor A; C009873:divinyl sulfone; D017228:Hepatocyte Growth Factor; D006820:Hyaluronic Acid; D006493:Heparin; D005022:Ethyldimethylaminopropyl Carbodiimide
keywords: Dopamine; Growth factor; Heparin; Hyaluronic acid; Re-endothelialization; Stent