title: Estrogenic effects of flavonoid components in Xiaoyao powder.
abstract: The objective of this study was to evaluate the estrogenic effects and mechanisms of three flavonoid components in Xiaoyao powder: quercetin, kaempferol, and isorhamnetin. The drugs were used to treat estrogen receptor (ER)-positive human breast cancer MCF-7 cells, and proliferation was measured using the MTT method. The expression of proteins and mRNA of the ER subtype were measured using western blotting and real time polymerase chain reaction. The quercetin (10(-2) μM, 10(-3) μM), kaempferol (100 μM, 10(-2) μM), and isorhamnetin (10(-3) μM) promoted the proliferation of MCF-7 cells, and the expression of ERα and ERβ proteins and mRNA were all increased significantly (P < 0.05). These effects were reversed by treatment with 0.1 μM estrogen antagonist ICI182780. Three flavonoid components in Xiaoyao powder increased the expression of proteins and mRNA of ERα and ERβ and promoted the proliferation of MCF-7 cells. These estrogenic effects were mediated by the ER.
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Chen
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang, China.
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang, China.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Wang
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang, China.
authors.forename: S X
authors.initials: SX
authors.lastname: Ji
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Dang
authors.affiliation: First Affiliated Hospital, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: S M
authors.initials: SM
authors.lastname: Li
authors.affiliation: First Affiliated Hospital, Jiamusi University, Jiamusi, Heilongjiang, China.
authors.forename: L
authors.initials: L
authors.lastname: Feng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D005492:Folic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D044949:Kaempferols / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D061986:MCF-7 Cells; D011208:Powders / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D044949:Kaempferols; D011208:Powders; D012333:RNA, Messenger; C516449:xiaoyao; C047368:3-methylquercetin; C006552:kaempferol; D005492:Folic Acid; D011794:Quercetin; C059966:ICI 198583