title: Molecular cloning, characterization, and the response of Cu/Zn superoxide dismutase and catalase to PBDE-47 and -209 from the freshwater bivalve Anodonta woodiana.
abstract: Polybrominated diphenyl ethers-47 (PBDE-47) and -209 are significant components of total PBDEs in water and can catalyze the production of reactive oxygen species (ROS) in the organisms. Anti-oxidant enzymes play an important role in scavenging the high level of ROS. In the current study, two full-length cDNAs of Cu/Zn superoxide dismutase (CuZnSODs) and catalase (CAT) were isolated from freshwater bivalve Anodonta woodiana by rapid amplification of cDNA ends approach and respectively named as AwSOD and AwCAT. The nucleotide sequence of AwSOD cDNA had an open reading frame (ORF) of 465 bp encoding a polypeptide of 155 amino acids in which signature 1 GKHGFHVHEFGDNT and signature 2 GNAGARSACGVI of SODs were observed. Deduced amino acid sequence of AwSOD showed a significant similarity with that of CuZnSODs. AwCAT had an ORF 1536 bp encoding a polypeptide of 512 amino acids which contains a conserved catalytic site motif, and a proximal heme-ligand signature motif of CATs. The time-course expressions of AwSOD and AwCAT in hepatopancreas were measured by quantitative real-time PCR. Expressions of AwSOD and AwCAT showed a significant up-regulation in groups at a low concentration treatment of PBDE-47, a biphasic pattern in groups with a high concentration treatment. Administration of PBDE-209 could result in an up-regulation of AwSOD and AwCAT expressions with time- and dose-dependent matter. These results indicate that up-regulations of AwSOD and AwCAT expression of hepatopancreas of freshwater bivalve A. woodiana contribute to eliminate oxidative stress derived from PBDE-47 and -209 treated.
authors.affiliation: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China; State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xiaxichao8336@163.com.
authors.forename: Xichao
authors.initials: X
authors.lastname: Xia
authors.affiliation: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Huang
authors.affiliation: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
authors.forename: Dongxian
authors.initials: D
authors.lastname: Zhang
authors.affiliation: The Third Affiliated Hospital of Henan Traditional Medicine University, Zhengzhou 45002, Henan Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Xue
authors.affiliation: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
authors.forename: Xiying
authors.initials: X
authors.lastname: Wang
authors.affiliation: Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: lhguo@rcees.ac.cn.
authors.forename: Lianghong
authors.initials: L
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049877:Anodonta / Q000187:drug effects* / Q000235:genetics; D001483:Base Sequence; D002374:Catalase / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005618:Fresh Water; D015870:Gene Expression / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D043143:Hepatopancreas / Q000187:drug effects / Q000378:metabolism; D000072105:Superoxide Dismutase-1 / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D055768:Halogenated Diphenyl Ethers; C511295:2,2',4,4'-tetrabromodiphenyl ether; D002374:Catalase; D000072105:Superoxide Dismutase-1; C010902:decabromobiphenyl ether
keywords: Anodonta woodiana; AwCAT; AwSOD; PBDE-209; PBDE-47