title: Influence of diabetes mellitus on the mineralization ability of two endodontic materials.
abstract: The aim of this study was to evaluate the influence of diabetes mellituson tissue response and mineralization ability of Sealapex®and MTA Fillapex® sealers. Twenty-four Wistar rats were divided into two groups: diabetic and non-diabetic. The materials were placed in polyethylene tubes and implanted into dorsal connective tissue of rats for 7 and 30 days. Six animals from each group received injection of calcein, alizarin, and oxytetracycline on days 7, 14, and 21, respectively. The animals were killed after 7 and 30 days and specimens were prepared for histologic analysis by staining with hematoxylin and eosin or Von Kossa or left unstained for polarized light or fluorescence microscopy. On day 7, inflammatory reactions were characterized. Moderate inflammatory responses were observed for all groups and on day 30, a mild inflammatory response against MTA Fillapex® and a moderate inflammatory response against Sealapex® were observed. Von Kossa-positive structures were observed in response to both materials and birefringent structures were observed upon polarized light analysis; these had no relation to the diabetic condition (p > 0.05). The fluorescence intensity was unaffected in diabetic rats (p > 0.05). In conclusion, diabetes mellitus did not influence the tissue response or mineralization stimulated by Sealapex® or MTA Fillapex®.
authors.affiliation: Department of Endodontics, Araçatuba School of Dentistry, Univ. Estadual Paulista, Araçatuba, SP, Brazil.
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes Filho
authors.affiliation: Department of Endodontics, Araçatuba School of Dentistry, Univ. Estadual Paulista, Araçatuba, SP, Brazil.
authors.forename: Índia Olinta de Azevedo
authors.initials: ÍO
authors.lastname: Queiroz
authors.affiliation: Department of Restorative Dentistry, Araçatuba School of Dentistry, Univ. Estaudal Paulista, Brasil.
authors.forename: Simone
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Department of Restorative Dentistry, Araçatuba School of Dentistry, Univ. Estaudal Paulista, Brasil.
authors.forename: Luciano Tavares Angelo
authors.initials: LT
authors.lastname: Cintra
authors.affiliation: Department of Restorative Dentistry, Araçatuba School of Dentistry, Univ. Estaudal Paulista, Brasil.
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004338:Drug Combinations; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008422:Materials Testing; D008856:Microscopy, Fluorescence; D010087:Oxides / Q000494:pharmacology*; D017208:Rats, Wistar; D012459:Salicylates / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012459:Salicylates; D017640:Silicates; C086631:mineral trioxide aggregate; C055601:Sealapex; D002126:Calcium Hydroxide