title: INVESTIGATION OF BIOFILM FORMATION IN COAGULASE-NEGATIVE STAPHYLOCOCCI ISOLATED FROM PLATELET CONCENTRATE BAGS.
abstract: Platelet Concentrates (PCs) are the blood components with the highest rate of bacterial contamination, and coagulase-negative staphylococci (CoNS) are the most frequently isolated contaminants. This study investigated the biofilm formation of 16 contaminated units out of 691 PCs tested by phenotypic and genotypic methods. Adhesion in Borosilicate Tube (ABT) and Congo Red Agar (CRA) tests were used to assess the presence of biofilm. The presence of icaADC genes was assessed by means of the Polymerase Chain Reaction (PCR) technique. With Vitek(r)2, Staphylococcus haemolyticus was considered the most prevalent CoNS (31.25%). The CRA characterized 43.8% as probable biofilm producers, and for the ABT test, 37.5%. The icaADC genes were identified in seven samples by the PCR. The ABT technique showed 85.7% sensitivity and 100% specificity when compared to the reference method (PCR), and presented strong agreement (k = 0.8). This study shows that species identified as PCs contaminants are considered inhabitants of the normal skin flora and they might become important pathogens. The results also lead to the recommendation of ABT use in laboratory routine for detecting biofilm in CoNS contaminants of PCs.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Rio Grande do Sul, Brasil, rosifarma@gmail.com; rosmari.ufsm@gmail.com; robertarampelotto@gmail.com; liti_razia@msn.com; melisenunes@hotmail.com.
authors.forename: Rosiéli
authors.initials: R
authors.lastname: Martini
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Rio Grande do Sul, Brasil, rosifarma@gmail.com; rosmari.ufsm@gmail.com; robertarampelotto@gmail.com; liti_razia@msn.com; melisenunes@hotmail.com.
authors.forename: Rosmari
authors.initials: R
authors.lastname: Hörner
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Rio Grande do Sul, Brasil, rosifarma@gmail.com; rosmari.ufsm@gmail.com; robertarampelotto@gmail.com; liti_razia@msn.com; melisenunes@hotmail.com.
authors.forename: Roberta Filipini
authors.initials: RF
authors.lastname: Rampelotto
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Rio Grande do Sul, Brasil, rosifarma@gmail.com; rosmari.ufsm@gmail.com; robertarampelotto@gmail.com; liti_razia@msn.com; melisenunes@hotmail.com.
authors.forename: Litiérri Razia Litiérri
authors.initials: LR
authors.lastname: Garzon
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Rio Grande do Sul, Brasil, rosifarma@gmail.com; rosmari.ufsm@gmail.com; robertarampelotto@gmail.com; liti_razia@msn.com; melisenunes@hotmail.com.
authors.forename: Melise Silveira
authors.initials: MS
authors.lastname: Nunes
authors.affiliation: Laboratório de Evolução Genética, Centro de Educação Superior Norte, Palmeira das Missões, Rio Grande do Sul, Brasil, teixeira.mayza@gmail.com; das.graichen@ufsm.br.
authors.forename: Mayza Dalcin
authors.initials: MD
authors.lastname: Teixeira
authors.affiliation: Laboratório de Evolução Genética, Centro de Educação Superior Norte, Palmeira das Missões, Rio Grande do Sul, Brasil, teixeira.mayza@gmail.com; das.graichen@ufsm.br.
authors.forename: Daniel Ângelo Sganzerla
authors.initials: DÂ
authors.lastname: Graichen
mesh_terms: D000362:Agar; D018441:Biofilms / Q000254:growth & development*; D001792:Blood Platelets / Q000382:microbiology*; D003030:Coagulase; D006801:Humans; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D003030:Coagulase; D000362:Agar