title: The development of dual-label time-resolved fluorescence immunoassay (TRFIA) for screening of ovarian cancer based on simultaneous detection of human epididymis protein-4 and cancer antigen 125.
abstract: A two-step dual-label TRFIA was developed for the simultaneous detection of human epididymis protein-4 and cancer antigen 125 in a single run. The performance of this assay was first evaluated using clinical serum samples, and then compared with commercialized kits. The sensitivity of this assay for cancer antigen 125 detection was 0.5 U/mL (dynamic range, 0-1400 U/L), and the sensitivity for human epididymis protein-4 detection was 1 pM (dynamic range, 1-900 pM). High correlation coefficients (R) were obtained between the present dual-label TRFIA and commercially available kits (R = 0.99). The present dual-label TRFIA has high sensitivity, specificity, and accuracy in clinical sample analysis. It is a good alternative to the single-label diagnostic methods.
authors.affiliation: a Cancer Center of The 88 Hospital of People's Liberation Army, Tai'an , Shandong , China.
authors.forename: Wen-Qiao
authors.initials: WQ
authors.lastname: Sun
authors.affiliation: b Shandong Medicine Technician College, Tai'an , Shandong , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: c Guangzhou Youdi Biotechnology Company, Guangzhou , Guangdong , China.
authors.forename: Xiao-Zhu
authors.initials: XZ
authors.lastname: Liu
authors.affiliation: c Guangzhou Youdi Biotechnology Company, Guangzhou , Guangdong , China.
authors.forename: Lai-Qing
authors.initials: LQ
authors.lastname: Li
authors.affiliation: c Guangzhou Youdi Biotechnology Company, Guangzhou , Guangdong , China.
authors.forename: Cui-Cui
authors.initials: CC
authors.lastname: Chen
authors.affiliation: a Cancer Center of The 88 Hospital of People's Liberation Army, Tai'an , Shandong , China.
authors.forename: Li-Cheng
authors.initials: LC
authors.lastname: Zhang
mesh_terms: D018394:CA-125 Antigen / Q000097:blood* / Q000276:immunology; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D013997:Time Factors; D000081102:WAP Four-Disulfide Core Domain Protein 2
chemical_list: D018394:CA-125 Antigen; D011506:Proteins; D000081102:WAP Four-Disulfide Core Domain Protein 2; C569698:WFDC2 protein, human
keywords: cancer antigen 125; dual-label TRFIA; human epididymis protein-4; ovarian cancer