title: The pleiotropic effects of the seed germination inhibitor germostatin.
abstract: Seed dormancy and germination are the most important adaptive traits of seed plants, which control the germination in a proper space and time. Internal genetic factors together with environmental cues govern seed dormancy and germination. Abscisic acid (ABA), a key phytohormone induces seed dormancy and inhibits seed germination through its molecular genetic signaling network responding the seed inherent physiological and environmental factors. Recently, auxin has been shown to be another phytohormone that induces seed dormancy. We have recently shown that germonstatin (GS), a small synthetic molecule identified by high through-put chemical genetic screenings, inhibits seed germination through up-regulating auxin signaling and inducing auxin biosynthesis. GERMOSTATIN RESISTANCE LOCUS 1 (GSR1) encodes a plant homeodomain (PHD) finger protein and is responsible for GS seed germination inhibition. Its knockdown mutant gsr1 displays decreased dormancy. In this report, we show that GS is not an ABA analog and provided 2 other GS-resistant mutants related to the chemical's function in seed germination inhibition other than gsr1, suggesting that GS may have pleiotropic effects through targeting different pathway governing seed germination.
authors.affiliation: a Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences , Shanghai , China.
authors.forename: Yajin
authors.initials: Y
authors.lastname: Ye
authors.affiliation: b Faculty of Life Science and Technology, Kunming University of Science and Technology , Yunnan , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000814:Aniline Compounds / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development*; D058685:Genetic Pleiotropy / Q000187:drug effects*; D018525:Germination / Q000187:drug effects*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*; D013440:Sulfides / Q000494:pharmacology*
chemical_list: D000814:Aniline Compounds; D013440:Sulfides; C000613154:germostatin; D000040:Abscisic Acid
keywords: ABA; auxin; germostatin; pleiotropic effects; seed germination