title: Transurethral instillation with fusion protein MrpH.FimH induces protective innate immune responses against uropathogenic Escherichia coli and Proteus mirabilis.
abstract: Urinary tract infections (UTIs) are among the most common infections in human. Innate immunity recognizes pathogen-associated molecular patterns (PAMPs) by Toll-like receptors (TLRs) to activate responses against pathogens. Recently, we demonstrated that MrpH.FimH fusion protein consisting of MrpH from Proteus mirabilis and FimH from Uropathogenic Escherichia coli (UPEC) results in the higher immunogenicity and protection, as compared with FimH and MrpH alone. In this study, we evaluated the innate immunity and adjuvant properties induced by fusion MrpH.FimH through in vitro and in vivo methods. FimH and MrpH.FimH were able to induce significantly higher IL-8 and IL-6 responses than untreated or MrpH alone in cell lines tested. The neutrophil count was significantly higher in the fusion group than other groups. After 6 h, IL-8 and IL-6 production reached a peak, with a significant decline at 24 h post-instillation in both bladder and kidney tissues. Mice instilled with the fusion and challenged with UPEC or P. mirabilis showed a significant decrease in the number of bacteria in bladder and kidney compared to control mice. The results of these studies demonstrate that the use of recombinant fusion protein encoding TLR-4 ligand represents an effective vaccination strategy that does not require the use of a commercial adjuvant. Furthermore, MrpH.FimH was presented as a promising vaccine candidate against UTIs caused by UPEC and P. mirabilis.
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mehri
authors.initials: M
authors.lastname: Habibi
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Asadi Karam
authors.affiliation: Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Bouzari
mesh_terms: D018829:Adhesins, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D018830:Adhesins, Escherichia coli / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D058491:Bacterial Load; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004927:Escherichia coli Infections / Q000517:prevention & control*; D005260:Female; D036781:Fimbriae Proteins / Q000008:administration & dosage* / Q000276:immunology; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D011512:Proteus Infections / Q000517:prevention & control*; D011513:Proteus mirabilis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology; D014551:Urinary Tract / Q000382:microbiology; D014552:Urinary Tract Infections / Q000517:prevention & control*; D056689:Uropathogenic Escherichia coli / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage
chemical_list: D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D001428:Bacterial Vaccines; D016207:Cytokines; C119575:MrpH protein, Proteus mirabilis; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C058769:fimH protein, E coli; D036781:Fimbriae Proteins
keywords: P. mirabilis; Urinary tract infection; fusion MrpH.FimH; innate immunity; toll-like receptor-4; uropathogenic Escherichia coli