title: Impedimetric aptasensor for nuclear factor kappa B with peroxidase-like mimic coupled DNA nanoladders as enhancer.
abstract: In this work, we developed a sensitive and universal aptasensor for nuclear factor kappa B (NF-κB) detection based on peroxidase-like mimic coupled DNA nanoladders for signal amplification. The dsDNA formed by capture DNA S1 and NF-κB binding aptamer (NBA) was firstly assembled on electrode surface. The presence of target NF-κB then led to the leave of NBA from electrode surface and thus provided the binding sites for immobilizing initiator to trigger in situ formation of DNA nanoladders on electrode surface. Since the peroxidase-like mimic manganese (III) meso-tetrakis (4-Nmethylpyridyl)-porphyrin (MnTMPyP) interacts with DNA nanoladders via groove binding, the insoluble benzo-4-chlorohexadienone (4-CD) precipitation derived from the oxidation of 4-chloro-1-naphthol (4-CN) could be formed on electrode surface in the presence of H2O2, resulting in a significantly amplified EIS signal output for quantitative target analysis. As a result, the developed aptasensor showed a low detection limit of 7pM and a wide linear range of 0.01-20nM. Featured with high sensitivity and label-free capability, the proposed sensing scheme can thus offer new opportunities for achieving sensitive, selective and stable detection of different types of target proteins.
authors.affiliation: Department of Kidney, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Kanfu
authors.initials: K
authors.lastname: Peng
authors.affiliation: Department of Kidney, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Hongwen
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Kidney, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Xie
authors.affiliation: Department of Kidney, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Hu
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Department of Kidney, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China. Electronic address: wuxfei@126.com.
authors.forename: Xiongfei
authors.initials: X
authors.lastname: Wu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D008665:Metalloporphyrins / Q000737:chemistry*; D016328:NF-kappa B / Q000032:analysis / Q000097:blood*; D009284:Naphthols / Q000737:chemistry; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D008665:Metalloporphyrins; C502230:Mn(III) 5,10,15,20-tetrakis(N-methylpyridinium-2-yl)porphyrin; D016328:NF-kappa B; D009284:Naphthols; C025917:4-chloro-1-naphthol; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: DNA nanoladders; Impedimetric aptasensor; Manganese (III) meso-tetrakis (4-Nmethylpyridyl)-porphyrin; Mimic enzyme catalyzed precipitation; Nuclear factor kappa B