title: Deep proteome analysis of gerontoplasts from the inner integument of developing seeds of Jatropha curcas.
abstract: The inner integument of Jatropha curcas seeds is a non-photosynthetic tissue that acts primarily as a conduit for the delivery of nutrients to the embryo and endosperm. In this study we performed a histological and transmission electron microscopy analysis of the inner integument in stages prior to fertilization to 25days after pollination, to establish the structural changes associated with the plastid to gerontoplast transition. This study showed that plastids are subjected to progressive changes, which include the dismantling of the internal membrane system, matrix degradation and the formation of stromule-derived vesicles. A proteome analysis of gerontoplasts isolated from the inner integument at 25days after pollination, resulted in the identification of 1923 proteins, which were involved in a myriad of metabolic functions, such as synthesis of amino acids and fatty acids. Among the identified proteins, were also a number of hydrolases (peptidases, lipases and carbohydrases), which presumably are involved in the ordered dismantling of this organelle to provide additional sources of nutrients for the growing embryo and endosperm. The dataset we provide here may provide a foundation for the study of the proteome changes associated with the plastid to gerontoplast transition in non-photosynthetic tissues.
  
  
  
  We describe ultrastructural features of gerontoplasts isolated from the inner integument of developing seeds of Jatropha curcas, together with a deep proteome analysis of these gerontoplasts. This article explores a new aspect of the biology of plastids, namely the ultrastructural and proteome changes associated with the transition plastid to gerontoplast in a non-photosynthetic tissue.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
authors.forename: Mohibullah
authors.initials: M
authors.lastname: Shah
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
authors.forename: Emanoella L
authors.initials: EL
authors.lastname: Soares
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
authors.forename: Magda L B
authors.initials: MLB
authors.lastname: Lima
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
authors.forename: Camila B
authors.initials: CB
authors.lastname: Pinheiro
authors.affiliation: Department of Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil.
authors.forename: Arlete A
authors.initials: AA
authors.lastname: Soares
authors.affiliation: Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-909, Rio de Janeiro, Brazil.
authors.forename: Gilberto B
authors.initials: GB
authors.lastname: Domont
authors.affiliation: Proteomic Unit, Institute of Chemistry, Federal University of Rio de Janeiro, 21941-909, Rio de Janeiro, Brazil. Electronic address: fabiocsn@ufrj.br.
authors.forename: Fabio C S
authors.initials: FCS
authors.lastname: Nogueira
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60455-900, Ceara, Brazil. Electronic address: bioplant@ufc.br.
authors.forename: Francisco A P
authors.initials: FAP
authors.lastname: Campos
mesh_terms: D031286:Jatropha / Q000737:chemistry / Q000196:embryology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D018087:Plastids / Q000737:chemistry / Q000648:ultrastructure; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D020543:Proteome
keywords: Lipases; Non-photosynthetic plastids; Peptidases; Programmed cell death