title: Thermoresponsive Epitope Surface-Imprinted Nanoparticles for Specific Capture and Release of Target Protein from Human Plasma.
abstract: Among various artificial antibodies, epitope imprinted polymer has been paid increasingly attention. To modulate the "adsorption and release" behavior by environment stimuli, N-isopropylacrylamide, was adopted to fabricate the thermoresponsive epitope imprinted sites. The prepared imprinted materials could adsorb 46.6 mg/g of target protein with the imprinting factor of 4.0. The template utilization efficiency could reach as high as 8.21%. More importantly, in the real sample, the materials could controllably capture the target protein from the human plasma at 45 °C and release it at 4 °C, which demonstrated the "on-demand" application potentials of such materials in the biomolecule recognition field.
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Senwu
authors.initials: S
authors.lastname: Li
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.
authors.forename: Kaiguang
authors.initials: K
authors.lastname: Yang
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Deng
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Min
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.|University of Chinese Academy of Sciences , Beijing 100049, China.|State Key Laboratory of Fine Chemicals, Dalian University of Technology , Dalian 116024, China.
authors.forename: Lukuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012709:Serum Albumin / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D001798:Blood Proteins; D000939:Epitopes; D012709:Serum Albumin; C052970:poly-N-isopropylacrylamide; D012822:Silicon Dioxide
keywords: controllable release; epitope; molecularly imprinting; protein; stimuli-response