title: Up-regulated expression of Ran reveals its potential role to deltamethrin stress in Kc cells.
abstract: The GTP-binding nuclear protein Ran has mostly been reported to be an essential player in nuclear transport, chromosome alignment, microtubule dynamics, centrosome duplication, kinetochore attachment of microtubules, nuclear-envelope dynamics, and phagocytosis. However, until now, there has been no report showing the involvement of Ran in DM stress. In this paper, two-dimensional electrophoresis analysis showed that the expression level of Ran in Kc cells in response to DM was higher than that in the control group. In addition, quantitative analysis using real-time PCR revealed that the expression of Ran was obviously up-regulated at various concentrations of DM. Western blot analysis showed that Ran was up-regulated 2.27-fold over the control at 48h. Because we still could not pinpoint whether Ran was actually involved in DM stress reaction, to further verify the role of Ran in stress reaction, RNA interference and cell transfection were utilized. Overexpression of Ran in cells conferred a degree of protection against DM after 72h. Furthermore, interference with Ran significantly decrease cell viability. All of the above findings strongly imply that Ran may participate in the development of stress reaction to DM. Therefore, investigating the possible role of Ran in DM stress will broaden our limited knowledge regarding DM stress inducible genes.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Energy and Mechanical Engineering, Nanjing Normal University, Nanjing 210042, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Chi
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Hu
authors.affiliation: Institute of Plant Protection, Guizhou Academy of Agricultural, Guiyang 550009, China.
authors.forename: Fengliang
authors.initials: F
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China. Electronic address: chengluogen@njnu.edu.cn.
authors.forename: Luogen
authors.initials: L
authors.lastname: Cheng
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000196:embryology / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D008658:Inactivation, Metabolic; D007305:Insecticide Resistance / Q000187:drug effects / Q000235:genetics; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects; D020931:ran GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D020931:ran GTP-Binding Protein
keywords: Deltamethrin; Kc cells; Ran; Stress reaction