title: Protective effects of resveratrol against cisplatin-induced testicular and epididymal toxicity in rats.
abstract: This study investigated the probable protective effect of resveratrol against cisplatin-induced testicular and epididymal toxicity in rats. Body weights of the animals showed no significant changes after cisplatin administration. Conversely, the weights of testis, and accessory sex organs reduced significantly. The daily sperm production and epididymal sperm quantity and quality were decreased in cisplatin treated rats. The circulatory levels of testosterone and activity levels of testicular 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase were significantly decreased after cisplatin treatment. The activity levels of superoxide dismutase and catalase were decreased with an increase in the levels of lipid peroxidation and H2O2 generation in the testis and epididymis of cisplatin treated rats, suggesting the cisplatin-induced oxidative stress. The biochemical findings were supplemented by histological examination of testis. Reduced tubular size, decreased spermatogenesis and deterioration in architecture were observed after cisplatin treatment. Administration of resveratrol alone has no significant effect on testicular and epididymal metabolism. On the other hand, administration of resveratrol ameliorated cisplatin-induced alterations in testicular and epididymal oxidative damage, suppressed steroiodgenesis and spermatogenesis and restored testicular architecture. In conclusion, resveratrol possesses multimechanistic protective activity that can be attributed to its steroidogenic and antioxidant actions.
authors.affiliation: Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, India; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
authors.forename: K Pratap
authors.initials: KP
authors.lastname: Reddy
authors.affiliation: Department of Biotechnology, Sri Venkateswara University, Tirupati 517502, India; Department of Zoology, Sri Venkateswara University, Tirupati 517502, India.
authors.forename: P
authors.initials: P
authors.lastname: Madhu
authors.affiliation: Department of Zoology, Sri Venkateswara University, Tirupati 517502, India. Electronic address: psreddy@svuniversity.ac.in.
authors.forename: P Sreenivasula
authors.initials: PS
authors.lastname: Reddy
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D013267:Stilbenes; D002945:Cisplatin; D000077185:Resveratrol
keywords: Cisplatin; Male rat; Oxidative stress; Resveratrol; Spermatogenesis; Steroidogenesis