title: Pro-apoptotic effect of new quinolone 7- ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo [3,4-h]quinoline-7-carboxylate on cervical cancer cell line HeLa alone/with UVA irradiation.
abstract: 7- ethyl 9-ethyl-6-oxo-6,9-dihydro[1,2,5]selenadiazolo [3,4-h]quinoline-7-carboxylate (E2h) is a new synthetically prepared quinolone derivative, which in our primary study showed cytotoxic effects towards tumor cells. The aim of the present study was to examine the antiproliferative and apoptosis inducing activities of E2h towards human cervical cancer cell line HeLa with/without the presence of UVA irradiation. Further, the molecular mechanism involved in E2h-induced apoptosis in HeLa cells was investigated. Our results showed that both non-photoactivated and photoactivated E2h caused morphological changes and inhibited the cell growth of HeLa cells in a time- and dose-dependent manner. Irradiation increased the sensitivity of HeLa cells to E2h. Quinolone induced S and G2/M arrest and apoptosis in HeLa cells, as characterized by DNA fragmentation and flow cytometry. In addition, E2h elevated the level of reactive oxygen species and activated caspases 3. In conclusions, E2h alone/in combination with UVA irradiation induced apoptosis in HeLa cells through the ROS-mitochondrial/caspase 3-dependent pathway.
authors.affiliation: Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic.
authors.forename: Soňa
authors.initials: S
authors.lastname: Jantová
authors.affiliation: Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic; Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovak Republic. Electronic address: natasa.mrvova@gmail.com.
authors.forename: Nataša
authors.initials: N
authors.lastname: Mrvová
authors.affiliation: Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Hudec
authors.affiliation: Cancer Research Institute, Slovak Academy of Science, Bratislava, Slovak Republic.
authors.forename: Ján
authors.initials: J
authors.lastname: Sedlák
authors.affiliation: Institute of Management, Slovak University of Technology, Bratislava, Slovak Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pánik
authors.affiliation: Institute of Organic Chemistry, Catalysis and Petrochemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovak Republic.
authors.forename: Viktor
authors.initials: V
authors.lastname: Milata
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000528:radiation effects; D016566:Organoselenium Compounds / Q000494:pharmacology*; D015363:Quinolones / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms
chemical_list: C000613243:7-ethyl 9-ethyl-6-oxo-6,9-dihydro(1,2,5)selenadiazolo(3,4-h)quinoline-7-carboxylate; D000970:Antineoplastic Agents; D016566:Organoselenium Compounds; D015363:Quinolones; D017382:Reactive Oxygen Species
keywords: Apoptosis; Cell cycle arrest; HeLa cells; Quinolone derivative; UVA irradiation