title: Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium.
abstract: In the olfactory epithelium (OE), olfactory cells (OCs) and supporting cells (SCs), which express different cadherins, are arranged in a characteristic mosaic pattern in which OCs are enclosed by SCs. However, the mechanism underlying this cellular patterning is unclear. Here, we show that the cellular pattern of the OE is established by cellular rearrangements during development. In the OE, OCs express nectin-2 and N-cadherin, and SCs express nectin-2, nectin-3, E-cadherin, and N-cadherin. Heterophilic trans-interaction between nectin-2 on OCs and nectin-3 on SCs preferentially recruits cadherin via α-catenin to heterotypic junctions, and the differential distributions of cadherins between junctions promote cellular intercalations, resulting in the formation of the mosaic pattern. These observations are confirmed by model cell systems, and various cellular patterns are generated by the combinatorial expression of nectins and cadherins. Collectively, the synergistic action of nectins and cadherins generates mosaic pattern, which cannot be achieved by a single mechanism.
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan Department of Otolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Katsunuma
authors.affiliation: Division of Neural Differentiation and Regeneration, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan Laboratory for Morphogenetic Signaling, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Honda
authors.affiliation: Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Shinoda
authors.affiliation: Department of Machine Intelligence and Systems Engineering, Akita Prefectural University, Akita 015-0055, Japan.
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ishimoto
authors.affiliation: Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Takaki
authors.initials: T
authors.lastname: Miyata
authors.affiliation: Animal Resource Development Unit, RIKEN Center for Life Science Technologies, Kobe 650-0047, Japan Genetic Engineering Team, Division of Bio-function Dynamics Imaging, RIKEN Center for Life Science Technologies, Kobe 650-0047, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyonari
authors.affiliation: Genetic Engineering Team, Division of Bio-function Dynamics Imaging, RIKEN Center for Life Science Technologies, Kobe 650-0047, Japan.
authors.forename: Takaya
authors.initials: T
authors.lastname: Abe
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nibu
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0047, Japan CREST, Japan Science and Technology Agency, Kobe 650-0047, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
authors.affiliation: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan htogashi@med.kobe-u.ac.jp.
authors.forename: Hideru
authors.initials: H
authors.lastname: Togashi
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000075983:Nectins; D009831:Olfactory Mucosa / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; C000619017:Nectin3 protein, mouse; D000075983:Nectins