title: One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
abstract: The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of modified strains (libraries). Despite the significance of yeast libraries and the immense variety of available tags and regulatory elements, only a few such libraries exist, as their construction is extremely expensive and laborious. To overcome these limitations, we developed a SWAp-Tag (SWAT) method that enables one parental library to be modified easily and efficiently to give rise to an endless variety of libraries of choice. To showcase the versatility of the SWAT approach, we constructed and investigated a library of ∼1,800 strains carrying SWAT-GFP modules at the amino termini of endomembrane proteins and then used it to create two new libraries (mCherry and seamless GFP). Our work demonstrates how the SWAT method allows fast and effortless creation of yeast libraries, opening the door to new ways of systematically studying cell biology.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Ido
authors.initials: I
authors.lastname: Yofe
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Uri
authors.initials: U
authors.lastname: Weill
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Meurer
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Silvia
authors.initials: S
authors.lastname: Chuartzman
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Einat
authors.initials: E
authors.lastname: Zalckvar
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Omer
authors.initials: O
authors.lastname: Goldman
authors.affiliation: Biological Services Department, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Shifra
authors.initials: S
authors.lastname: Ben-Dor
authors.affiliation: Institut für Biochemie und Molekularbiologie, ZBMZ Universität, Freiburg, Germany.
authors.forename: Conny
authors.initials: C
authors.lastname: Schütze
authors.affiliation: Institut für Biochemie und Molekularbiologie, ZBMZ Universität, Freiburg, Germany.|BIOSS Centre for Biological Signalling Studies, Universität Freiburg, Freiburg, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Wiedemann
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.|Cell Morphogenesis and Signal Transduction, Deutsches Krebsforschungszentrum (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Knop
authors.affiliation: Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
authors.forename: Anton
authors.initials: A
authors.lastname: Khmelinskii
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Maya
authors.initials: M
authors.lastname: Schuldiner
mesh_terms: D015153:Blotting, Western; D003585:Cell Biology; D015723:Gene Library; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D020675:Peroxisomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins