title: Effects of Fluoride on the Expression of p38MAPK Signaling Pathway-Related Genes and Proteins in Spleen Lymphocytes of Mice.
abstract: This study investigated the effects of sodium fluoride on the expression of p38MAPK signaling pathway-related genes and proteins in the spleen lymphocytes of mice, revealing the mechanism of the toxicity of fluoride to the immune system. The spleen lymphocytes, isolated from mice consuming different NaF doses (0, 50, 100, and 150 mg/L) for 60 days, were cultured in medium with bacteria lipopolysaccharide, and the cells' proliferation ability was analyzed through MTT; real-time PCR detected the change of MLK3/MKK6/p38MAPK/MSK1/ATF1 on mRNA, and the difference of protein expression of MKK6/p38MAPK were detected through the Western blotting. The results suggested that the proliferation ability of spleen lymphocytes isolated from mice consuming different NaF doses is lower, and the expression of genes and proteins of MKK6/p38MAPK showed a decreasing trend. These results demonstrate that fluoride can suppress the activation of p38MAPK pathway in mice spleen lymphocytes and further influences the function of the immune system.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Jinzhong, Shanxi, (030800), China.
authors.forename: Zeyu
authors.identifier: http://orcid.org/0000-0002-2773-9185
authors.initials: Z
authors.lastname: Shi
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Jinzhong, Shanxi, (030800), China.
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Jinzhong, Shanxi, (030800), China.
authors.forename: Junxing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Jinzhong, Shanxi, (030800), China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Jinzhong, Shanxi, (030800), China. mahaili1718@126.com.
authors.forename: Haili
authors.initials: H
authors.lastname: Ma
mesh_terms: D000818:Animals; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000276:immunology*
chemical_list: D048051:p38 Mitogen-Activated Protein Kinases; D005459:Fluorides
keywords: Fluoride; Gene expression; Protein expression; Spleen lymphocytes; p38MAPK