title: Effects of Common Fig (Ficus carica) Leaf Extracts on Sperm Parameters and Testis of Mice Intoxicated with Formaldehyde.
abstract: Formaldehyde (FA) is the leading cause of cellular injury and oxidative damage in testis that is one of the main infertility causes. There has been an increasing evidence of herbal remedies use in male infertility treatment. This assay examines the role of Ficus carica (Fc) leaf extracts in sperm parameters and testis of mice intoxicated with FA. Twenty-five adult male mice were randomly divided into control; sham; FA-treated (10 mg/kg twice per day); Fc-treated (200 mg/kg); and FA + Fc-treated groups. Cauda epididymal spermatozoa were analyzed for viability, count, and motility. Testes were weighed and gonadosomatic index (GSI) was calculated. Also, histoarchitecture of seminiferous tubules was assessed in the Haematoxylin and Eosin stained paraffin sections. The findings showed that FA significantly decreased GSI and increased percentage of immotile sperm compared with control group. Disorganized and vacuolated seminiferous epithelium, spermatogenic arrest, and lumen filled with immature germ cells were also observed in the testes. However, Fc leaf extracts improved sperm count, nonprogressive motility of spermatozoa, and GSI in FA-treated testes. Moreover, seminiferous tubule with spermatogenic arrest was rarely seen, indicating that Fc has the positive effects on testis and epididymal sperm parameters exposed with FA.
authors.affiliation: Department of Anatomical Sciences, Medicine School, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Naghdi
authors.affiliation: Department of Pathology, Faculty of Medicine, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Maghbool
authors.affiliation: Department of Animal Biosystematics, Shahid Beheshti University, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Seifalah-Zade
authors.affiliation: Department of Anatomical Sciences, Medicine School, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mahaldashtian
authors.affiliation: Department of Anatomical Sciences, Medicine School, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Makoolati
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
authors.forename: Seyed Amin
authors.initials: SA
authors.lastname: Kouhpayeh
authors.affiliation: Department of Health Education and Promotion, School of Public Health, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Ghasemi
authors.affiliation: Department of Anatomical Sciences, Medicine School, Fasa University of Medical Sciences, Fasa 7461686688, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Fereydouni