title: Decreased miRNA-146a in glioblastoma multiforme and regulation of cell proliferation and apoptosis by target Notch1.
abstract: OBJECTIVE
  The primary purpose of this paper is to investigate the relationship between the microRNA 146a (miR-146a) and the proliferation of cells occurring in glioblastoma multiforme. The secondary purpose of the paper is to investigate abnormalities of expression in miR-146a.
  
  
  METHODS
  A real-time PCR assay was used to investigate the abnormal expression of miR-146a in glioma and adjacent tissue. Lipofection was used to transfect a mimic of miR-146a and induce the upregulation of miR-146a. Real-time PCR was used to observe the expression level of miR-146a. A cell viability analysis was conducted using MTT. A luciferase report vector was used to identify potential targets for miR-146a.
  
  
  RESULTS
  The miR-146a component was found to be downregulated in glioma tissue compared with adjacent nontumor tissue (p&lt;0.05). The upregulation of miR-146a in glioma cells through miR-146a mimic transfection led to reduction of cell viability and to an increase in the percentage of apoptosis. Notch1 was the name of the potential targeted gene for miR-146a in glioma.
  
  
  CONCLUSIONS
  The study found that the presence of miR-146a potentially affected the proliferation of glioma cells by regulating the rate of Notch1 expression.
authors.affiliation: Department of Neurosurgery, Xinxiang Central Hospital, Xinxiang - P.R. China.
authors.forename: Hong-Qi
authors.initials: HQ
authors.lastname: Hu
authors.affiliation: Department of Neurosurgery, Xinxiang Central Hospital, Xinxiang - P.R. China.
authors.forename: Lai-Guang
authors.initials: LG
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Xinxiang Central Hospital, Xinxiang - P.R. China.
authors.forename: Wu-Jun
authors.initials: WJ
authors.lastname: Guo
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D051881:Receptor, Notch1 / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1