title: [Effect of Emodin Derivative E11 on T Lymphocytic Leukemia Cell Line Molt-4 and Its Possible Mechanisms].
abstract: OBJECTIVE
  To explore the effect of a new emodin derivative E11 on proliferation and apoptosis of T lymphocytic leukemia cell line Molt-4 and its possible mechanisms.
  
  
  METHODS
  MTT method was used to plot cell growth curve. Colony culture assay was performed for studying the effect of emodin derivative E11 on colony-formation of Molt-4. The fluorescent microscopy with DAPI staining was used to examine the cell morphological changes after E11 treatment. DNA fragmentation method was used to detect the inducing effect of emodin derivative E11 on cell apoptosis. Western blot was used to determine the expressions of apoptosis-related proteins including procaspase-9, procaspase-3, PARP and PI3K/AKT, MAPK signalling pathway.
  
  
  RESULTS
  Emodin derivative E11 could strongly inhibit the growth of Molt-4 with the IC50 in 48 h at 1.381 ± 0.1552 µmol/L in dose-dependent manner. 0.1 µmol/L of E11 could inhibit cell colony formation. The typrical apopototic morphologic changes of Molt cells treated with E11 could be observed under fluorescence microscope with DAPI staining. DNA apoptotic ladder could be observed by DNA fragmentation.The expressions of procaspase -9, procaspase-3, PARP, p-MAPK, p-AKT, mTOR, p-mTOR, p-P70 and p-4BEP1 were down-regulated, while expressions of MAPK, AKT, 4EBP1 and P70 were not changed remarkably after Molt-4 were treated with E11 for 48 h.
  
  
  CONCLUSIONS
  E11 can remarkably inhibit the proliferation and induce the apoptosis of Molt-4 cells. The mechanism of apoptosis of Molt-4 cells may be related with the suppression of PI3K/AKT and MAPK signalling pathways.
authors.affiliation: Union Clinical Medical College, Fujian Medical University, Fuzhou 350001, Fujian Province, China.|Department of Tumor, Jinjiang Municipal Hospital, Jinjiang 362200, Fujian Province, China.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Huang
authors.affiliation: Department of Chemistry, School of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, Fujian Province, China.
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Wang
authors.affiliation: Union Clinical Medical College, Fujian Medical University, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Key Laboratory of Hematology, Department of Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China. E-mail: jdhu@medmail.com.cn.
authors.forename: Jian-Da
authors.initials: JD
authors.lastname: Hu
authors.affiliation: Union Clinical Medical College, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
authors.forename: Jun-Ting
authors.initials: JT
authors.lastname: Zheng
authors.affiliation: Union Clinical Medical College, Fujian Medical University, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Key Laboratory of Hematology, Department of Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation; D015536:Down-Regulation; D004642:Emodin / Q000494:pharmacology*; D006801:Humans; D015458:Leukemia, T-Cell / Q000473:pathology*; D020935:MAP Kinase Signaling System; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
chemical_list: D053148:Caspase 3; D053453:Caspase 9; D004642:Emodin; D019869:Phosphatidylinositol 3-Kinases; D011065:Poly(ADP-ribose) Polymerases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C546842:MTOR protein, human; C505407:CASP3 protein, human; C506433:CASP9 protein, human