title: Intraoperative Identification of Liver Cancer Microfoci Using a Targeted Near-Infrared Fluorescent Probe for Imaging-Guided Surgery.
abstract: Difficulties in the highly sensitive detection of tumour microfoci represent a critical obstacle toward improved surgical intervention in liver cancer. Conventional preoperative imaging methods and surgeons' subjective experience are limited by their inability to effectively detect tumour lesions measuring less than 2 mm; however, intraoperative fluorescence molecular imaging may overcome this limitation. Here, we synthesised an arginine-glycine-aspartic acid (RGD)-conjugated mesoporous silica nanoparticle (MSN) highly loaded with indocyanine green (ICG) dye that could accurately delineate liver cancer margins and provide excellent tumour-to-normal tissue contrast intraoperatively. The increased ICG loading capacity and tumour specificity enabled the identification of residual microtumours and satellite lesions measuring less than 1 mm in living mice. Histological analysis validated the sensitivity and accuracy of this approach. We believe this technique utilising a new fluorescent nanoprobe with intraoperative optical imaging may offer a more sensitive and accurate method for liver cancer resection guidance, resulting in better surgical outcomes.
authors.affiliation: Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.|Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Chaoting
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.|Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Shang
authors.affiliation: Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.|Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.|Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Chongwei
authors.initials: C
authors.lastname: Chi
authors.affiliation: Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.|Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jia
authors.affiliation: Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Fang
authors.affiliation: Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.|Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Du
authors.initials: D
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Jinzuo
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
authors.forename: Chihua
authors.initials: C
authors.lastname: Fang
authors.affiliation: Key Laboratory of Molecular Imaging, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.|Beijing Key Laboratory of Molecular Imaging, Beijing 100190, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D005456:Fluorescent Dyes; D056945:Hep G2 Cells; D064593:Heterografts; D006801:Humans; D007208:Indocyanine Green; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000601:surgery*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D009842:Oligopeptides; D061848:Optical Imaging / Q000379:methods*; D012822:Silicon Dioxide; D025321:Surgery, Computer-Assisted / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; D012822:Silicon Dioxide; C047981:arginyl-glycyl-aspartic acid; D007208:Indocyanine Green