title: Endo-xylanase and endo-cellulase-assisted extraction of pectin from apple pomace.
abstract: Pectins were extracted from apple pomace with monoactive preparation of endo-xylanase and endo-cellulase. The process was conducted for 10 h in conditions of pH 5.0 at 40 °C, with constant shaking. Endo-xylanase application resulted in the highest extraction efficiency of pectins (19.8%). The obtained polymer was characterised by a very high molecular mass, high level of neutral sugars - mainly arabinose, galactose and glucose, and very high DM (73.4). It also contained the highest level of protein and phenols. Pectin extracted with endo-cellulase had 1.5 fold lower molecular mass but contained significantly more GalA (70.5%) of a high degree of methylation (66.3%). The simultaneous application of both enzymatic preparations resulted in their cooperation, leading to a decrease of both the extraction efficiency and the molecular mass of pectin. However, this pectin was distinguished by the highest GalA (74.7%) and rhamnose contents.
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, ul. Balicka 122, 31-149 Kraków, Poland. Electronic address: a.wikiera@ur.krakow.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wikiera
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, ul. Balicka 122, 31-149 Kraków, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mika
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, ul. Balicka 122, 31-149 Kraków, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Starzyńska-Janiszewska
authors.affiliation: Department of Food Biotechnology, Faculty of Food Technology, Agricultural University of Cracow, ul. Balicka 122, 31-149 Kraków, Poland.
authors.forename: Bożena
authors.initials: B
authors.lastname: Stodolak
mesh_terms: D001089:Arabinose / Q000032:analysis / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005503:Food Additives / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005690:Galactose / Q000032:analysis / Q000378:metabolism; D006603:Hexuronic Acids / Q000032:analysis / Q000378:metabolism; D027845:Malus / Q000737:chemistry* / Q000378:metabolism; D008745:Methylation; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005503:Food Additives; D006603:Hexuronic Acids; D010636:Phenols; D010940:Plant Proteins; C007819:galacturonic acid; D010368:Pectins; D001089:Arabinose; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D005690:Galactose
keywords: Byproducts; Cell wall polysaccharides; Enzymatic extraction; Pectin composition