title: Toxic Diatom Aldehydes Affect Defence Gene Networks in Sea Urchins.
abstract: Marine organisms possess a series of cellular strategies to counteract the negative effects of toxic compounds, including the massive reorganization of gene expression networks. Here we report the modulated dose-dependent response of activated genes by diatom polyunsaturated aldehydes (PUAs) in the sea urchin Paracentrotus lividus. PUAs are secondary metabolites deriving from the oxidation of fatty acids, inducing deleterious effects on the reproduction and development of planktonic and benthic organisms that feed on these unicellular algae and with anti-cancer activity. Our previous results showed that PUAs target several genes, implicated in different functional processes in this sea urchin. Using interactomic Ingenuity Pathway Analysis we now show that the genes targeted by PUAs are correlated with four HUB genes, NF-κB, p53, δ-2-catenin and HIF1A, which have not been previously reported for P. lividus. We propose a working model describing hypothetical pathways potentially involved in toxic aldehyde stress response in sea urchins. This represents the first report on gene networks affected by PUAs, opening new perspectives in understanding the cellular mechanisms underlying the response of benthic organisms to diatom exposure.
authors.affiliation: Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Varrella
authors.affiliation: Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Romano
authors.affiliation: CROM, Istituto Nazionale Tumori "Fondazione G. Pascale," IRCCS, Napoli, Italy.
authors.forename: Susan
authors.initials: S
authors.lastname: Costantini
authors.affiliation: Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Ruocco
authors.affiliation: Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Ianora
authors.affiliation: Faculty of Science and Technology, Bournemouth University, Talbot Campus, Poole, United Kingdom.
authors.forename: Matt G
authors.initials: MG
authors.lastname: Bentley
authors.affiliation: Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, Napoli, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Costantini
mesh_terms: D000447:Aldehydes / Q000633:toxicity*; D000818:Animals; D017377:Diatoms / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D053263:Gene Regulatory Networks / Q000187:drug effects*; D012617:Sea Urchins / Q000187:drug effects* / Q000196:embryology / Q000235:genetics*
chemical_list: D000447:Aldehydes