title: Up-regulated ephrinB3/EphB3 expression in intractable temporal lobe epilepsy patients and pilocarpine induced experimental epilepsy rat model.
abstract: EphB family receptor tyrosine kinases, in cooperation with cell surface-bound ephrinB ligands, play a critical role in maintenance of dendritic spine morphogenesis, axons guidance, synaptogenesis, synaptic reorganization and plasticity in the central nervous system (CNS). However, the expression pattern of ephrinB/EphB in intractable temporal lobe epilepsy (TLE) and the underlying molecular mechanisms during epileptogenesis remain poorly understood. Here we investigated the expression pattern and cellular distribution of ephrinB/EphB in intractable TLE patients and lithium chloride-pilocarpine induced TLE rats using real-time quantitative polymerase chain reaction (RT-qPCR), immunohistochemistry, double-labeled immunofluorescence and Western blot analysis. Compared to control groups, ephrinB3 and EphB3 mRNA expression were significantly up-regulated in intractable TLE patients and TLE rats, while the mRNA expression trend of ephrinB1/2 and EphB1/2/4/6 in intractable TLE patients and TLE rats were inconsistent. Western blot analysis and semi-quantitative immunohistochemistry confirmed that ephrinB3 and EphB3 protein level were up-regulated in intractable TLE patients and TLE rats. At the same time, double-labeled immunofluorescence indicate that ephrinB3 was expressed mainly in the cytoplasm and protrusions of glia and neurons, while EphB3 was expressed mainly in the cytoplasm of neurons. Taken together, up-regulated expression of ephrinB3/EphB3 in intractable TLE patients and experimental TLE rats suggested that ephrinB3/EphB3 might be involved in the pathogenesis of TLE.
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Ruohan
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Jinxian
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Ou
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Neurology, Second Affiliated Hospital of Chongqing Medical University, 74 Lin Jiang Road, Chongqing 400010, China. Electronic address: chenym1997@sina.com.
authors.forename: Yangmei
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D004195:Disease Models, Animal; D000069279:Drug Resistant Epilepsy / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D036389:Ephrin-B3 / Q000378:metabolism*; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010862:Pilocarpine; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D036223:Receptor, EphB3 / Q000378:metabolism*; D013702:Temporal Lobe / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D055815:Young Adult
chemical_list: D036389:Ephrin-B3; D012333:RNA, Messenger; D010862:Pilocarpine; D036223:Receptor, EphB3
keywords: EphB3; EphrinB3; Pilocarpine; Synaptic plasticity; Synaptogenesis; Temporal lobe epilepsy