title: [First report of complete genome sequence and phylogenetic analysis of Human Bocavirus 1 isolated in Argentina].
abstract: BACKGROUND
  Human bocavirus (HBoV) is a parvovirus identified for the first time in 2005 associated to upper- and lower- acute respiratory tract infection (ARI), which is one of the main causes of morbimortality in infant population worldwide. Currently four genotypes have been described named HBoV1-4, of which HBoV1 is the one predominantly related to ARI.
  
  
  OBJECTIVE
  To obtain the complete genome of respiratory HBoV locally isolated.
  
  
  METHODS
  By means of bioinformatics tools such as ClustalW and NCBI Primer-Blast, primers were designed to amplify overlapping DNA fragments altogether spanning the complete genome of HBoV. Fragments were amplified by PCR and sequenced by BigDye Terminator capillary technology. Sequence editing and phylogenetic analysis were accomplished using MEGA v6 software.
  
  
  RESULTS
  Complete genome sequence of HBoV1 strain 307AR09 was obtained after isolation from respiratory secretion of a pediatric patient with bronchiolitis. The sequence was deposited in the GenBank public database (accession number KJ634207). The phylogenetic analysis including complete genome sequences of all four genotypes from around the world shows similarity close to 100% between the local strain and the virus originally discovered in Sweden (DQ000495). The four genotypes clustered in 2 groups of high internal homology: HBoV1-HBoV3 and HBoV2-HBoV4.
  
  
  CONCLUSIONS
  We provide local molecular data that can be used in future technological developments for research and diagnostic tests intended for medical practice. Our results add support to the proposed redistribution of the four genotypes into 2 species.
authors.forename: Agustina
authors.initials: A
authors.lastname: Cardozo Tomas
authors.forename: Lucia Maria
authors.initials: LM
authors.lastname: Ghietto
authors.forename: Constanza
authors.initials: C
authors.lastname: Insfran
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wasinger
authors.forename: Ariana
authors.initials: A
authors.lastname: Marchesi
authors.forename: Maria Pilar
authors.initials: MP
authors.lastname: Adamo
mesh_terms: D001118:Argentina; D016679:Genome, Viral / Q000235:genetics*; D056661:Human bocavirus / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012141:Respiratory Tract Infections / Q000821:virology*; D017422:Sequence Analysis, DNA