title: Pentoxifylline affects idarubicin binding to DNA.
abstract: Anticancer drug idarubicin - derivative of doxorubicin - is commonly used in treatment of numerous cancer types. However, in contrast to doxorubicin, its biophysical properties are not well established yet. Additionally, potential direct interactions of idarubicin with other biologically active aromatic compounds, such as pentoxifylline - representative of methylxanthines - were not studied at all. Potential formation of such hetero-aggregates may result in sequestration of the anticancer drug and, in consequence, reduction of its biological activity. This work provide description of the idarubicin biophysical properties as well as assess influence of pentoxifylline on idarubicin interactions with DNA. To achieve these goals we employed spectrophotometric methods coupled with analysis with the appropriate mathematical models as well as flow cytometry and Ames test. Obtained results show influence of pentoxifylline on idarubicin binding to DNA and are well in agreement with the data previously published for other aromatic ligands. Additionally it may be hypothesized that direct interactions between idarubicin and pentoxifylline may influence the anticancer drug biological activity.
authors.affiliation: Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Gołuński
authors.affiliation: Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Borowik
authors.affiliation: Laboratory of Virus Molecular Biology, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
authors.forename: Andrea
authors.initials: A
authors.lastname: Lipińska
authors.affiliation: Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Romanik
authors.affiliation: Laboratory of Virus Molecular Biology, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Derewońko
authors.affiliation: Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Woziwodzka
authors.affiliation: Laboratory of Biophysics, Intercollegiate Faculty of Biotechnology UG-MUG, Abrahama 58, 80-307 Gdańsk, Poland. Electronic address: piosik@biotech.ug.edu.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Piosik
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D015255:Idarubicin / Q000737:chemistry*; D015394:Molecular Structure; D010431:Pentoxifylline / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D004247:DNA; C427203:calf thymus DNA; D010431:Pentoxifylline; D015255:Idarubicin
keywords: Ames test; Flow cytometry; Hetero-aggregation; Methylxanthines; Stacking complexes