title: The investigation of different pollutants and operation processes on sludge toxicity in sequencing batch bioreactors.
abstract: The influence of different target pollutants and operation modes in sequencing batch bioreactors (SBRs) on sludge toxicity was compared in this study. Sludge toxicity was characterized by the inhibiting luminosity through using luminescent bacterium Photobacterium phosphoreum (P. phosphoreum) during either gradual acclimation or impaction processes with synthetic wastewater containing high-strength bisphenol A (BPA) or N, N-dimethylformamide (DMF). When the activated sludge was first acclimated with either 120 mg/L DMF or 20 mg/L BPA, and then respectively increased to 200 mg/L DMF and 40 mg/L BPA it was defined as gradual acclimation process, whereas when the activated sludge was, respectively, injected with 200 mg/L DMF and 40 mg/L BPA directly it was defined as impaction process. Results showed that the toxicity of the impacted sludge was greater than that of the gradual acclimated sludge, especially in the initial stage before 10 d. Activated sludge treating BPA synthetic wastewater exhibited higher toxicity due to the more inhibition of BPA to sludge activity compared to that of DMF. The proteomics analysis indicated that the stress responses of activated sludge to DMF and BPA stimulation were both significant. In turn, the secretions from two kinds of sludge under stress conditions contributed to sludge toxicity.
authors.affiliation: a State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process , East China University of Science and Technology , Shanghai 200237 , People's Republic of China.
authors.forename: Xiurong
authors.initials: X
authors.lastname: Chen
authors.affiliation: a State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process , East China University of Science and Technology , Shanghai 200237 , People's Republic of China.|b Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education , Henan Key Laboratory for Environmental Pollution Control, Henan Normal University , Xinxiang 453007 , People's Republic of China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhao
authors.affiliation: b Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education , Henan Key Laboratory for Environmental Pollution Control, Henan Normal University , Xinxiang 453007 , People's Republic of China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Bao
authors.affiliation: a State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process , East China University of Science and Technology , Shanghai 200237 , People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: a State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process , East China University of Science and Technology , Shanghai 200237 , People's Republic of China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis* / Q000737:chemistry; D019149:Bioreactors; D004126:Dimethylformamide / Q000032:analysis* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D012722:Sewage / Q000032:analysis / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004126:Dimethylformamide; D010636:Phenols; D012722:Sewage; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: DMF and BPA synthetic wastewater; gradual acclimation process; impaction process; proteomics analysis; sludge toxicity