title: Differential expression of the lethal gene Luteus-Pa in cacao of the Parinari series.
abstract: The recessive lethal character Luteus-Pa is found in cacao (Theobroma cacao) genotypes of the Parinari series (Pa) and is characterized by expression of leaf chlorosis and seedling death. Several genotypes of the Pa series are bearers of the gene responsible for the expression of the Luteus-Pa character, which can be used as a tool for determining relationships between genotypes of this group. To evaluate this phenomenon, we analyzed the differential expression of genes between mutant seedlings and wild-type hybrid Pa 30 x 169 seedlings, with the aim of elucidating the possible lethal mechanisms of the homozygous recessive character Luteus-Pa. Plant material was harvested from leaves of wild and mutant seedlings at different periods to construct a subtractive library and perform quantitative analysis using real-time PCR. The 649 sequences obtained from the subtractive library had an average length of 500 bp, forming 409 contigs. The probable proteins encoded were grouped into 10 functional categories. Data from ESTs identified genes associated with Rubisco, peroxidases, and other proteins and enzymes related to carbon assimilation, respiration, and photosystem 2. Mutant seedlings were characterized by synthesizing defective PsbO and PsbA proteins, which were overexpressed from 15 to 20 days after seedling emergence.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: B C
authors.initials: BC
authors.lastname: Rehem
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: A-A F
authors.initials: AA
authors.lastname: Almeida
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: G S F
authors.initials: GS
authors.lastname: Figueiredo
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária, Cruz das Almas, BA, Brasil.
authors.forename: A S
authors.initials: AS
authors.lastname: Gesteira
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: S C
authors.initials: SC
authors.lastname: Santos
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: R X
authors.initials: RX
authors.lastname: Corrêa
authors.affiliation: Centro de Pesquisas do Cacau, Comissão Executiva do Plano da Lavoura Cacaueira, Itabuna, BA, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: Yamada
authors.affiliation: Centro de Pesquisas do Cacau, Comissão Executiva do Plano da Lavoura Cacaueira, Itabuna, BA, Brasil.
authors.forename: R R
authors.initials: RR
authors.lastname: Valle
mesh_terms: D002099:Cacao / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins