title: Regulatory and junctional proteins of the blood-testis barrier in human Sertoli cells are modified by monobutyl phthalate (MBP) and bisphenol A (BPA) exposure.
abstract: The blood-testis barrier (BTB) is responsible for providing a protected environment and coordinating the spermatogenesis. Endocrine disruptors (EDs) might lead to infertility, interfering in the BTB structure and modulation. This study aimed to correlate the actions of two EDs, monobutyl phthalate (MBP) and bisphenol A (BPA) in different periods of exposure, in a low toxicity dose to the human Sertoli cells (HSeC) and its effects on the proteins of the BTB and regulatory proteins involved in its modulation. HSeC cells were exposed to MBP (10μM) and BPA (20μM) for 6 and 48h. Western Blot assay indicated that MBP was able to reduce the expression of occludin, ZO-1, N-cadherin and Androgen Receptor (AR), while BPA leads to a reduction of occludin, ZO-1, β-catenin and AR. TGF-β2 and F-actin were not modified. Phalloidin and Hematoxylin and Eosin assay revealed phenotically disruption in Sertoli cells adhesion, without changes in F-actin expression or localization. Our data suggested both EDs present potential for disrupting the structure and maintenance of the human BTB by AR dependent pathway.
authors.affiliation: Graduate Program in General and Applied Biology, Institute of Biosciences, UNESP, Botucatu, SP, Brazil.
authors.forename: André Teves Aquino Gonçalves
authors.initials: ATAG
authors.lastname: de Freitas
authors.affiliation: Graduate Program in General and Applied Biology, Institute of Biosciences, UNESP, Botucatu, SP, Brazil.
authors.forename: Mariana Antunes
authors.initials: MA
authors.lastname: Ribeiro
authors.affiliation: Graduate Program in General and Applied Biology, Institute of Biosciences, UNESP, Botucatu, SP, Brazil.
authors.forename: Cristiane Figueiredo
authors.initials: CF
authors.lastname: Pinho
authors.affiliation: Graduate Program in General and Applied Biology, Institute of Biosciences, UNESP, Botucatu, SP, Brazil.
authors.forename: André Rebelo
authors.initials: AR
authors.lastname: Peixoto
authors.affiliation: Department of Anatomy, Institute of Biosciences, UNESP, Botucatu, SP, Brazil.
authors.forename: Raquel Fantin
authors.initials: RF
authors.lastname: Domeniconi
authors.affiliation: Department of Morphology, Institute of Biosciences, UNESP, Botucatu, SP, Brazil. Electronic address: scarano@ibb.unesp.br.
authors.forename: Wellerson R
authors.initials: WR
authors.lastname: Scarano
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000633:toxicity*; D001814:Blood-Testis Barrier / Q000187:drug effects / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D006801:Humans; D008297:Male; D062793:Occludin / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D010795:Phthalic Acids / Q000633:toxicity*; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013091:Spermatogenesis; D062826:Zonula Occludens-1 Protein / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D015703:Antigens, CD; D001559:Benzhydryl Compounds; D015820:Cadherins; D052244:Endocrine Disruptors; D062793:Occludin; D010636:Phenols; D010795:Phthalic Acids; D062826:Zonula Occludens-1 Protein; D051176:beta Catenin; C050361:CDH2 protein, human; C495270:CTNNB1 protein, human; C568985:OCLN protein, human; C569042:TJP1 protein, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C028577:monobutyl phthalate
keywords: (HSeC) Human Sertoli cells; Bisphenol A; Blood-testis barrier; Endocrine disruptors; Male reproductive function; Monobutyl phthalate