title: Structural analysis of novel kestose isomers isolated from sugar beet molasses.
abstract: Eight kestose isomers were isolated from sugar beet molasses by carbon-Celite column chromatography and HPLC. GC-FID and GC-MS analyses of methyl derivatives, MALD-TOF-MS measurements and NMR spectra were used to confirm the structural characteristics of the isomers. The (1)H and (13)C NMR signals of each isomer saccharide were assigned using COSY, E-HSQC, HSQC-TOCSY, HMBC and H2BC techniques. These kestose isomers were identified as α-D-fructofuranosyl-(2- > 2)-α-D-glucopyranosyl-(1 < ->2)-β-D-fructofuranoside, α-D-fructofuranosyl-(2- > 3)-β-D-fructofuranosyl-(2 < ->1)-α-D-glucopyranoside, α-D-fructofuranosyl-(2- > 4)-β-D-fructofuranosyl-(2 < ->1)-α-D-glucopyranoside, β-D-fructofuranosyl-(2- > 4)-β-D-fructofuranosyl-(2 < ->1)-α-D-glucopyranoside, β-D-fructofuranosyl-(2- > 3)-α-D-glucopyranosyl-(1 < ->2)-β-D-fructofuranoside, α-D-fructofuranosyl-(2- > 1)-β-D-fructofuranosyl-(2 < ->1)-α-D-glucopyranoside, α-D-fructofuranosyl-(2- > 6)-α-D-glucopyranosyl-(1 < ->2)-β-D-fructofuranoside, and α-D-fructofuranosyl-(2- > 6)-β-D-fructofuranosyl-(2 < ->1)-α-D-glucopyranoside. The former five compounds are novel saccharides.
authors.affiliation: Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Shiomi
authors.affiliation: Research Center, Nippon Beet Sugar Mfg. Co., Ltd., Obihiro 080-0831, Japan. Electronic address: abet@nitten.co.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Abe
authors.affiliation: Research Center, Nippon Beet Sugar Mfg. Co., Ltd., Obihiro 080-0831, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kikuchi
authors.affiliation: Research Center, Nippon Beet Sugar Mfg. Co., Ltd., Obihiro 080-0831, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Aritsuka
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takata
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Fukushi
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yukiharu
authors.initials: Y
authors.lastname: Fukushi
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
authors.affiliation: Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Ueno
authors.affiliation: Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Onodera
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry* / Q000201:enzymology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D008965:Molasses; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D014312:Trisaccharides
keywords: Beet molasses; Kestose isomer; Oligosaccharide