title: The Genetic Linkage Map of the Medicinal Mushroom Agaricus subrufescens Reveals Highly Conserved Macrosynteny with the Congeneric Species Agaricus bisporus.
abstract: Comparative linkage mapping can rapidly facilitate the transfer of genetic information from model species to orphan species. This macrosynteny analysis approach has been extensively used in plant species, but few example are available in fungi, and even fewer in mushroom crop species. Among the latter, the Agaricus genus comprises the most cultivable or potentially cultivable species. Agaricus bisporus, the button mushroom, is the model for edible and cultivable mushrooms. We have developed the first genetic linkage map for the basidiomycete A. subrufescens, an emerging mushroom crop known for its therapeutic properties and potential medicinal applications. The map includes 202 markers distributed over 16 linkage groups (LG), and covers a total length of 1701 cM, with an average marker spacing of 8.2 cM. Using 96 homologous loci, we also demonstrated the high level of macrosynteny with the genome of A. bisporus The 13 main LG of A. subrufescens were syntenic to the 13 A. bisporus chromosomes. A disrupted synteny was observed for the three remaining A. subrufescens LG. Electronic mapping of a collection of A. subrufescens expressed sequence tags on A. bisporus genome showed that the homologous loci were evenly spread, with the exception of a few local hot or cold spots of homology. Our results were discussed in the light of Agaricus species evolution process. The map provides a framework for future genetic or genomic studies of the medicinal mushroom A. subrufescens.
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France mfoulong@bordeaux.inra.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Foulongne-Oriol
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France Departamento de Biologia, UFLA, Universidade Federal de Lavras, C.P. 3037, 37200-000, Lavras, MG, Brazil.
authors.forename: Manuela
authors.initials: M
authors.lastname: Rocha de Brito
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Cabannes
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Clément
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France.
authors.forename: Cathy
authors.initials: C
authors.lastname: Spataro
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Moinard
authors.affiliation: Departamento de Biologia, UFLA, Universidade Federal de Lavras, C.P. 3037, 37200-000, Lavras, MG, Brazil.
authors.forename: Eustáquio Souza
authors.initials: ES
authors.lastname: Dias
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Callac
authors.affiliation: INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, Villenave d'Ornon, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Savoie
mesh_terms: D000364:Agaricus / Q000235:genetics*; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D019295:Computational Biology / Q000379:methods; D019143:Evolution, Molecular; D023061:Gene Order; D008040:Genetic Linkage; D016681:Genome, Fungal; D023281:Genomics / Q000379:methods; D018895:Microsatellite Repeats; D026801:Synteny
keywords: almond mushroom; button mushroom; chromosome evolution; comparative mapping; macrosynteny