title: Identification of polymorphisms and transcriptional activity of the proto-oncogene KIT located on both autosomal and B chromosomes of the Chinese raccoon dog.
abstract: B chromosomes are dispensable and co-exist with autosomal and sex chromosomes. The karyotype of the Chinese raccoon dog (Nyctereutes procyonoides procyonoides) comprises 0-4 B chromosomes. The proto-oncogene KIT is found on all B chromosomes of the Chinese raccoon dog. In the present study, partial DNA and mRNA sequences of KIT were amplified and sequenced from four individuals containing B chromosomes. Sequence analyses revealed that polymorphisms including single nucleotide polymorphisms (SNPs) and inserts/deletions were rich in the KIT gene of Chinese raccoon dog at the genomic level. However, no polymorphism was detected at the mRNA level. A comparison of mRNA sequences from Chinese raccoon dogs with the corresponding sequences derived from arctic fox and dog, which do not contain B chromosomes, revealed the mRNA sequences of the 10 SNPs to be identical between these three species. Therefore, these findings suggest that KIT located on the B chromosomes in Chinese raccoon dog lacks transcriptional activity.
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Zhu
authors.affiliation: College of Animal Science, Jilin University, Changchun, China.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Yan
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Sun
mesh_terms: D000818:Animals; D001483:Base Sequence; D033481:Chromosomes, Mammalian; D054643:INDEL Mutation; D020641:Polymorphism, Single Nucleotide; D019009:Proto-Oncogene Proteins c-kit / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D046031:Raccoon Dogs / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017421:Sequence Analysis; D014158:Transcription, Genetic
chemical_list: D019009:Proto-Oncogene Proteins c-kit