title: Antisense expression of Gossypium hirsutum UDP-glucuronate decarboxylase in Arabidopsis leads to changes in cell wall components.
abstract: UDP-glucuronate decarboxylase (UDP-xylose synthase; UXS, EC 4.1.1.35) is an essential enzyme of the non-cellulosic polysaccharide biosynthetic pathway. In the present study, using transient expression of fluorescently labeled Gossypium hirsutum UXS (GhUXS3) protein in onion epidermal cells, we observed that this protein was distributed in the cytoplasm. The GhUXS3 cDNA of cotton was expressed in an antisense orientation in Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation. Homozygous plants showing down-regulation of UXS were analyzed with northern blots. Compared to the untransformed control, transgenic plant showed shorter roots, earlier blossom formation, and delayed senescence. Biochemical analysis indicated that levels of rhamnose, mannose, galactose, glucose, xylose, and cellulose were reduced in some of the down-regulated antisense plants. These results suggest that GhUXS3 regulates the conversion of non-cellulosic polysaccharides and modulates their composition in plant cell walls. We also discuss a possible cellular function for GhUXS in determining the quality of cotton fibers.
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: D M
authors.initials: DM
authors.lastname: Zhang
authors.affiliation: North China University Science and Technology, Tangshan, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Pan
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: Z K
authors.initials: ZK
authors.lastname: Li
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Wu
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: H W
authors.initials: HW
authors.lastname: Liu
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: G Y
authors.initials: GY
authors.lastname: Zhang
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.affiliation: North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Key Laboratory of Crop Germplasm Resources of Hebei, Agricultural University of Hebei, Baoding, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Ma
mesh_terms: D000375:Aging; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D050260:Carbohydrate Metabolism / Q000235:genetics*; D002262:Carboxy-Lyases / Q000235:genetics*; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D016373:DNA, Antisense; D018744:DNA, Plant; D035264:Flowers / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics; D018517:Plant Roots / Q000033:anatomy & histology; D030821:Plants, Genetically Modified
chemical_list: D016373:DNA, Antisense; D018744:DNA, Plant; D002262:Carboxy-Lyases; C023257:UDPglucuronate decarboxylase