title: Maternal Omega-3 Supplement Improves Dopaminergic System in Pre- and Postnatal Inflammation-Induced Neurotoxicity in Parkinson's Disease Model.
abstract: Evidence suggests that idiopathic Parkinson's disease (PD) is the consequence of a neurodevelopmental disruption, rather than strictly a consequence of aging. Thus, we hypothesized that maternal supplement of omega-3 polyunsaturated fatty acids (ω-3 PUFA) may be associated with neuroprotection mechanisms in a self-sustaining cycle of neuroinflammation and neurodegeneration in lipopolysaccharide (LPS)-model of PD. To test this hypothesis, behavioral and neurochemical assay were performed in prenatally LPS-exposed offspring at postnatal day 21. To further determine whether prenatal LPS exposure and maternal ω-3 PUFAs supplementation had persisting effects, brain injury was induced on PN 90 rats, following bilateral intranigral LPS injection. Pre- and postnatal inflammation damage not only affected dopaminergic neurons directly, but it also modified critical features, such as activated microglia and astrocyte cells, disrupting the support provided by the microenvironment. Unexpectedly, our results failed to show any involvement of caspase-dependent and independent apoptosis pathway in neuronal death mechanisms. On the other hand, learning and memory deficits detected with a second toxic exposure were significantly attenuated in maternal ω-3 PUFAs supplementation group. In addition, ω-3 PUFAs promote beneficial effect on synaptic function, maintaining the neurochemical integrity in remaining neurons, without necessarily protect them from neuronal death. Thus, our results suggest that ω-3 PUFAs affect the functional ability of the central nervous system in a complex way in a multiple inflammation-induced neurotoxicity animal model of PD and they disclose new ways of understanding how these fatty acids control responses of the brain to different challenges.
authors.affiliation: Laboratório de Neurofisiologia, Setor de Ciências Biológicas, Departamento de Fisiologia, Universidade Federal do Paraná, Av. Francisco H. dos Santos s/n, 81.531 - 990, Curitiba, PR, Brazil. amdelattre@hotmail.com.
authors.forename: Ana Marcia
authors.initials: AM
authors.lastname: Delattre
authors.affiliation: Laboratório de Neurofisiologia, Setor de Ciências Biológicas, Departamento de Fisiologia, Universidade Federal do Paraná, Av. Francisco H. dos Santos s/n, 81.531 - 990, Curitiba, PR, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Carabelli
authors.affiliation: Laboratório de Neurofisiologia, Setor de Ciências Biológicas, Departamento de Fisiologia, Universidade Federal do Paraná, Av. Francisco H. dos Santos s/n, 81.531 - 990, Curitiba, PR, Brazil.
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Mori
authors.affiliation: Laboratório de Neurofisiologia, Setor de Ciências Biológicas, Departamento de Fisiologia, Universidade Federal do Paraná, Av. Francisco H. dos Santos s/n, 81.531 - 990, Curitiba, PR, Brazil.
authors.forename: Paula G
authors.initials: PG
authors.lastname: Kempe
authors.affiliation: Laboratório de Neurobiologia, Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Rizzo de Souza
authors.affiliation: Laboratório de Neurobiologia, Departamento de Patologia Básica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Silvio M
authors.initials: SM
authors.lastname: Zanata
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Ricardo B
authors.initials: RB
authors.lastname: Machado
authors.affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Deborah
authors.initials: D
authors.lastname: Suchecki
authors.affiliation: Laboratório de Neurofisiologia, Departamento de Fisiologia e Farmacologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Brazil.
authors.forename: Belmira L S
authors.initials: BLS
authors.lastname: Andrade da Costa
authors.affiliation: Laboratório de Neurofisiologia, Setor de Ciências Biológicas, Departamento de Fisiologia, Universidade Federal do Paraná, Av. Francisco H. dos Santos s/n, 81.531 - 990, Curitiba, PR, Brazil.
authors.forename: Marcelo M S
authors.initials: MMS
authors.lastname: Lima
authors.affiliation: Laboratório de Neurofisiologia, Setor de Ciências Biológicas, Departamento de Fisiologia, Universidade Federal do Paraná, Av. Francisco H. dos Santos s/n, 81.531 - 990, Curitiba, PR, Brazil. anete@ufpr.br.
authors.forename: Anete C
authors.initials: AC
authors.lastname: Ferraz
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019587:Dietary Supplements; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D007249:Inflammation / Q000178:diet therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage; D010300:Parkinson Disease / Q000178:diet therapy* / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D039401:Prenatal Nutritional Physiological Phenomena / Q000187:drug effects / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015525:Fatty Acids, Omega-3; D018696:Neuroprotective Agents
keywords: Dopaminergic system; Fish oil; Inflammation; LPS; Neuroprotection; Omega-3; Parkinson’s disease