title: Drosophila Fed ARA and EPA Yields Eicosanoids, 15S-Hydroxy-5Z,8Z, 11Z, 13E-Eicosatetraenoic Acid, and 15S-Hydroxy-5Z,8Z,11Z,13E,17Z-Eicosapentaenoic Acid.
abstract: Drosophila melanogaster has been a widely used as a model system for its powerful genetic tools. However, it remains to be illustrated if Drosophila can be used to examine the biochemical and physiological metabolism of eicosanoids. Thus, the analysis on the metabolism of C20 polyunsaturated fatty acids (PUFA) in Drosophila was implemented with high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Fatty acid (FA) analysis of the whole body, head, and thorax-abdomen in Drosophila showed C20 PUFA could only be found in Drosophila fed diets supplemented with eicosapentaenoic acid (EPA) and arachidonic acid (ARA), but not in Drosophila fed base diets. The C20 PUFA were found in abundance in the head. Drosophila fed ARA- and EPA-supplemented diets yielded 15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid [15(S)-HETE] and 15S-hydroxy-5Z,8Z,11Z,13E,17Z-eicosapentaenoic acid [15(S)-HEPE], respectively, while other sampled eicosanoids could not be detected. Similar results were obtained by incubating fly tissue supplemented with ARA or EPA. Furthermore, a genome sequence scan indicated that no gene encoding the key enzymes synthesizing eicosanoids were found in Drosophila. These findings demonstrate that Drosophila may possess a special lipid metabolic system, which is different from mammals.
authors.affiliation: Zhejiang Key Laboratory for Agro-Food Processing, Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Tan
authors.affiliation: Zhejiang Key Laboratory for Agro-Food Processing, Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Xin
authors.affiliation: Zhejiang Key Laboratory for Agro-Food Processing, Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhai
authors.affiliation: Zhejiang Key Laboratory for Agro-Food Processing, Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, China. shenlirong@zju.edu.cn.
authors.forename: Lirong
authors.initials: L
authors.lastname: Shen
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D016718:Arachidonic Acid / Q000008:administration & dosage / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D019587:Dietary Supplements; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000302:isolation & purification*; D053719:Tandem Mass Spectrometry
chemical_list: D029721:Drosophila Proteins; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid
keywords: Arachidonic acid; Drosophila melanogaster; Eicosanoids; Eicosapentaenoica cid; High performance liquid chromatography tandem mass spectrometry; Lipoxygenases