title: Postprandial kinetics of gene expression of proteins involved in the digestive process in rainbow trout (O. mykiss) and impact of diet composition.
abstract: The impact of increased incorporation of plant ingredients on diets for rainbow trout was evaluated in terms of gene expression of gastric (gastric lipase, pepsinogen) and intestinal (prolidase, maltase, phospholipase A2) digestive enzymes and nutrient transporters (peptide and glucose transporters), as well as of postprandial levels of plasma glucose, triglycerides and total free amino acids. For that purpose, trout alevins were fed from the start of exogenous feeding one of three different experimental diets: a diet rich in fish meal and fish oil (FM-FO), a plant-based diet (noFM-noFO) totally free from fish meal and fish oil, but containing plant ingredients and a Mixed diet (Mixed) intermediate between the FM-FO and noFM-noFO diets. After 16 months of rearing, all fish were left unfed for 72 h and then given a single meal to satiation. Blood, stomach and anterior intestine were sampled before the meal and at 2, 6 and 12 h after this meal. The postprandial kinetics of gene expression of gastric and intestinal digestive enzymes and nutrient transporters were then followed in trout fed the FM-FO diet. The postprandial profiles showed that the expression of almost all genes studied was stimulated by the presence of nutrients in the digestive tract of trout, but the timing (appearance of peaks) varied between genes. Based on these data, we have focused on the molecular response to dietary factors in the stomach and the intestine at 6 and 12 h after feeding, respectively. The reduction in FM and FO levels of dietary incorporation induced a significant decrease in the gene expression of gastric lipase, GLUT2 and PEPT1. The plasma glucose and triglycerides levels were also reduced in trout fed the noFM-noFO diet. Consequently, the present study suggests a decrease in digestive capacities in trout fed a diet rich in plant ingredients.
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.|EEM, UMR 5254 IPREM, Equipe Environnement et Microbiologie, Université de Pau et des Pays de l'Adour, 64013, Pau, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Borey
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Panserat
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Surget
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Cluzeaud
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Plagnes-Juan
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Herman
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Viviana
authors.initials: V
authors.lastname: Lazzarotto
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Corraze
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Médale
authors.affiliation: EEM, UMR 5254 IPREM, Equipe Environnement et Microbiologie, Université de Pau et des Pays de l'Adour, 64013, Pau, France.
authors.forename: Beatrice
authors.initials: B
authors.lastname: Lauga
authors.affiliation: UMR1419 Nutrition, Métabolisme, Aquaculture, INRA, F-64310, Saint Pee sur Nivelle, France. cburel@st-pee.inra.fr.
authors.forename: Christine
authors.identifier: http://orcid.org/0000-0001-8586-573X
authors.initials: C
authors.lastname: Burel
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004032:Diet; D004063:Digestion / Q000235:genetics*; D005395:Fish Oils; D005396:Fish Products; D029941:Fish Proteins / Q000235:genetics*; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D051273:Glucose Transporter Type 2 / Q000235:genetics; D006867:Hydrolases / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000097:blood / Q000235:genetics* / Q000502:physiology; D000074011:Peptide Transporter 1; D010938:Plant Oils; D010940:Plant Proteins; D019518:Postprandial Period / Q000235:genetics*; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics; D027981:Symporters / Q000235:genetics; D014280:Triglycerides / Q000097:blood
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D005395:Fish Oils; D029941:Fish Proteins; D051273:Glucose Transporter Type 2; D000074011:Peptide Transporter 1; D010938:Plant Oils; D010940:Plant Proteins; D051269:Sodium-Glucose Transporter 1; D027981:Symporters; D014280:Triglycerides; D006867:Hydrolases
keywords: Digestive enzymes; Nutrient transporters; Plant-based diet; Rainbow trout