title: Effect of co-culturing human primary basic fibroblasts with respiratory syncytial virus-infected 16-HBE cells.
abstract: This study investigated the effects induced by co-culturing human primary basic fibroblasts (HPBFs) with 16-human bronchial epithelial cells (16-HBE) infected with respiratory syncytial virus (RSV), in particular the transformation of HPBFs into myofibroblasts and secretion of extracellular matrix proteins. HPBFs were co-cultured with 16-HBE cells infected with RSV and quantitatively analyzed. We constructed models of HPBFs co-cultured with 16-HBE cells that were either uninfected (control group) or infected with RSV (experimental group). Following initiation of co-cultures, HPBFs and supernatants were collected at 24-h intervals up to 120 h. Expression of α-smooth muscle actin (α-SMA) was detected by indirect immunofluorescence and western blotting, while type I collagen (Col I) and fibronectin were analyzed by competitive enzyme-linked immunosorbent assays. After 72 h, α-SMA expression increased in HPBFs cultured with RSV-infected 16-HBE relative to uninfected controls, reaching its highest level at 96 h. Similarly, Col I secretion was also higher in HPBFs co-cultured with RSV-infected 16-HBE relative to uninfected controls; Col I secretion increased with time and reached its highest level at 120 h. HPBFs were transformed into myofibroblasts following co-culture with RSV-infected 16-HBE, which when combined with the observed increase in Col I secretion suggests that airway remodeling would then be promoted.
authors.affiliation: Guangzhou Panyu Central Hospital, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, China.
authors.forename: J-H
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Guangzhou Panyu Central Hospital, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, China.
authors.forename: L-H
authors.initials: LH
authors.lastname: Sun
authors.affiliation: Guangzhou Panyu Central Hospital, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, China.
authors.forename: S-K
authors.initials: SK
authors.lastname: Huang
authors.affiliation: Guangzhou Panyu Central Hospital, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, China.
authors.forename: A-H
authors.initials: AH
authors.lastname: Chen
mesh_terms: D000199:Actins / Q000378:metabolism; D056151:Airway Remodeling; D001980:Bronchi / Q000473:pathology / Q000821:virology; D018920:Coculture Techniques; D024042:Collagen Type I / Q000378:metabolism; D004847:Epithelial Cells / Q000473:pathology / Q000821:virology*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D058628:Myofibroblasts / Q000378:metabolism / Q000473:pathology*; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D012136:Respiratory Syncytial Viruses; D012141:Respiratory Tract Infections / Q000378:metabolism / Q000473:pathology* / Q000821:virology
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D005353:Fibronectins