title: Inhibitory effects of S-allylmercaptocysteine against benzo(a)pyrene-induced precancerous carcinogenesis in human lung cells.
abstract: The anti-cancer effects of oil-soluble organosulfur compounds in garlic in the initiation phase of carcinogenesis are known. However, there are few experimental studies investigating S-allylmercaptocysteine (SAMC), a water-soluble derivative of garlic. This study investigated whether SAMC prevented the carcinogen benzo(a)pyrene (B(a)P) from inducing precancerous activity in human lung cells (A549 cell line). A549 cells were either pre-treated (PreTM) or concurrently treated (CoTM) with 1μM B(a)P and either 10 or 50 μM SAMC. The 50 μM PreTM group inhibited B(a)P-induced cell proliferation by approximately 100%. The 50 μM SAMC PreTM and CoTM inhibited the B(a)P-induced G2/M phase shift by 100% and 97%, respectively. Furthermore, the PreTM and CoTM groups exhibited the potential to reduce the generation of reactive oxygen species (ROS) relative to the B(a)P group by at least 78%. The SAMC PreTM elevated superoxide dismutase (SOD) by approximately 100%. In this study, we revealed the mechanisms involved in SAMC inhibition of B(a)P-induced carcinogenesis, including suppression of cell proliferation, cell cycle regulation, attenuation of ROS formation, inhibition of DNA damage, increase of SOD activity and inhibition of nuclear factor-kappa B (NF-κB) activity. SAMC appears to be a novel therapeutic candidate for the prevention and treatment of B(a)P-induced human lung cancer.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China.
authors.forename: Qiuchen
authors.initials: Q
authors.lastname: Qi
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China; School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Daxue Road, Western University Science Park, Jinan, Shandong 250353, PR China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China.
authors.forename: Guangpu
authors.initials: G
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Luan
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan, Shandong 250012, PR China; Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, China. Electronic address: zxzhao@sdu.edu.cn.
authors.forename: Zhongxi
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cai
authors.affiliation: Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, China.
authors.forename: Jimin
authors.initials: J
authors.lastname: Cao
authors.affiliation: Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, China.
authors.forename: Shanzhong
authors.initials: S
authors.lastname: Li
mesh_terms: D000072283:A549 Cells; D001564:Benzo(a)pyrene / Q000378:metabolism; D063646:Carcinogenesis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D005737:Garlic / Q000276:immunology; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy*; D016328:NF-kappa B / Q000378:metabolism*; D011230:Precancerous Conditions; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species; C086300:S-allylmercaptocysteine; D001564:Benzo(a)pyrene; D013482:Superoxide Dismutase; D003545:Cysteine
keywords: A549; Benzo(a)pyrene; Cancer therapy; NF-κB; ROS; S-allylmercaptocysteine