title: New Delhi metallo-β-lactamase in Jamaica.
abstract: BACKGROUND
  The global dissemination of the New Delhi metallo-beta-lactamase (NDM) gene among certain strains of bacteria has serious implications since the infections caused by such organisms pose a therapeutic challenge. Although the NDM gene has been detected in various parts of the world, this is the first report of its detection in the English-speaking Caribbean. The NDM producing Klebsiella pneumoniae was isolated from an Indian patient who had recently relocated to Jamaica.
  
  
  METHODS
  Identification and susceptibility testing of the K. pneumoniae isolate was performed using the Vitek 2 automated system) in keeping with Clinical and Laboratory Standards Institute (CLSI) standards. It was identified as a metallobetalactamase producer using the Rosco KPC+MBL kit. Genotypic screening for common betalactamase (including carbapenemase) genes, was carried out  using two multiplex PCRs: one for SHV-, TEM-, CTX-M-, OXA-1-, and CMY-2-types, and one for VIM-, KPC-, IMP-, OXA-48, GES-, and NDM-types. Strain typing was conducted by pulsed-field gel electrophoresis (PFGE) using XbaI and multi-locus sequencing (MLS). Plasmid isolation and analysis was also performed.
  
  
  RESULTS
  K. pneumoniae (N11-02395), not previously associated with the dissemination of the NDM in India, Sweden or the UK, was found to harbor the NDM-1 gene on plasmid pNDM112395.
  
  
  CONCLUSIONS
  The identification of the NDM-1 gene underscores the need for effective surveillance and infection control measures to identify and prevent spread of multidrug resistant Gram negative bacilli. Strict infection control measures implemented for this patient helped to prevent the spread of this organism to other patients.
authors.affiliation: University of West Indies, Kingston, Jamaica. camille.thomsrodriguez@uwimona.edu.jm.
authors.forename: Camille-Ann
authors.initials: CA
authors.lastname: Thoms-Rodriguez
authors.forename: Tony
authors.initials: T
authors.lastname: Mazzulli
authors.forename: Nicole
authors.initials: N
authors.lastname: Christian
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Willey
authors.forename: David A
authors.initials: DA
authors.lastname: Boyd
authors.forename: Laura F
authors.initials: LF
authors.lastname: Mataseje
authors.forename: Michael R
authors.initials: MR
authors.lastname: Mulvey
authors.forename: Celia D C
authors.initials: CD
authors.lastname: Christie
authors.forename: Alison M
authors.initials: AM
authors.lastname: Nicholson
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007223:Infant; D007563:Jamaica; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D010957:Plasmids / Q000032:analysis; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C552864:beta-lactamase NDM-1