title: Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals.
abstract: The overall serological prevalence of EV infections based on ELISA remains unknown. In the present study, the antibody responses against VP1 of the EV-A species (enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A5 (CA5) and Coxsackievirus A6 (CA6)), of the EV-B species (Coxsackievirus B3 (CB3)), and of the EV-C species (Poliovirus 1 (PV1)) were detected and analyzed by a NEIBM (novel evolved immunoglobulin-binding molecule)-based ELISA in Shanghai blood donors. The serological prevalence of anti-CB3 VP1 antibodies was demonstrated to show the highest level, with anti-PV1 VP1 antibodies at the second highest level, and anti-CA5, CA6, CA16 and EV71 VP1 antibodies at a comparatively low level. All reactions were significantly correlated at different levels, which were approximately proportional to their sequence similarities. Antibody responses against EV71 VP1 showed obvious differences with responses against other EV-A viruses. Obvious differences in antibody responses between August 2013 and May 2014 were revealed. These findings are the first to describe the detailed information of the serological prevalence of human antibody responses against the VP1 of EV-A, B and C viruses, and could be helpful for understanding of the ubiquity of EV infections and for identifying an effective approach for seroepidemiological surveillance based on ELISA.
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Caixia
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Physiology, Anhui Medical University, Hefei, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Blood Transfusion, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Qian
authors.affiliation: Department of Physiology, Anhui Medical University, Hefei, China.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Mingmei
authors.initials: M
authors.lastname: Cao
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Heng
authors.initials: H
authors.lastname: Peng
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Bing
authors.initials: B
authors.lastname: Rui
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medicine, Second Military Medical University.
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
mesh_terms: D000914:Antibodies, Viral; D000917:Antibody Formation; D002681:China / Q000453:epidemiology; D004770:Enterovirus / Q000276:immunology*; D004769:Enterovirus Infections / Q000453:epidemiology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D014764:Viral Proteins
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins