title: Lipogenic Enzymes Complexes and Cytoplasmic Lipid Droplet Formation During Adipogenesis.
abstract: Lipid droplets are dynamic organelles that store triglycerides and participate in their mobilization in adipose cells. These organelles require the reorganization of some structural components, the cytoskeleton, and the activation of lipogenic enzymes. Using confocal microscopy, we analyzed the participation of cytoskeletal components and two lipogenic enzymes, fatty acid synthase and glycerophosphate dehydrogenase, during lipid droplet biogenesis in differentiating 3T3-F442A cells into adipocytes. We show that subcortical actin microfilaments are extended at the basal side of the cells in parallel arrangement to the culture dish substrate, and that the microtubule network traverses the cytoplasm as a scaffold that supports the round shape of the mature adipocyte. By immunoprecipitation, we show that vimentin and perilipin1a associate during the early stages of the differentiation process for lipid droplet formation. We also report that the antibody against perilipin1 detected a band that might correspond to a modified form of the molecule. Finally, the cytosolic distribution and punctate organization of lipogenic enzymes and their co-localization in the proximity of lipid droplets suggest the existence of dynamic protein complexes involved in synthesis and storage of triglycerides. J. Cell. Biochem. 117: 2315-2326, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies-IPN (CINVESTAV-IPN), México City 07360, Mexico.
authors.forename: Teresita
authors.initials: T
authors.lastname: Padilla-Benavides
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies-IPN (CINVESTAV-IPN), México City 07360, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Velez-delValle
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies-IPN (CINVESTAV-IPN), México City 07360, Mexico.
authors.forename: Meytha
authors.initials: M
authors.lastname: Marsch-Moreno
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies-IPN (CINVESTAV-IPN), México City 07360, Mexico.
authors.forename: Federico
authors.initials: F
authors.lastname: Castro-Muñozledo
authors.affiliation: Department of Cell Biology, Center for Research and Advanced Studies-IPN (CINVESTAV-IPN), México City 07360, Mexico.
authors.forename: Walid
authors.initials: W
authors.lastname: Kuri-Harcuch
mesh_terms: D000199:Actins / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000502:physiology*; D015153:Blotting, Western; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D066292:Lipid Droplets / Q000502:physiology*; D050155:Lipogenesis / Q000502:physiology; D000070778:Perilipin-1 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014404:Tubulin / Q000378:metabolism; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D000070778:Perilipin-1; D012333:RNA, Messenger; D014404:Tubulin; D014746:Vimentin; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+); D064429:Fatty Acid Synthases
keywords: ADIPOCYTES; ADIPOSE TISSUE; LIPOGENESIS; LIPOGENIC ENZYMES; PERILIPIN; VIMENTIN