title: [RANKL increases on peripheral blood T lymphocytes and serum Dickkopf1 decreases in rheumatoid arthritis].
abstract: OBJECTIVE
  To explore the roles of bone metabolism-related molecules in bone metabolic disturbance and disease progression of patients with rheumatoid arthritis (RA).
  
  
  METHODS
  A total of 66 RA patients and 20 healthy controls were included, and their relevant clinical information was gathered. Electrochemiluminescence immunoassay (ECLIA) was used to detect the levels of osteocalcin N-terminal middle (OC-N-MID) and C-terminal cross-linked telopeptides of type 1 collagen (CTX) in serum. The serum levels of Wnt inhibitory factor Dickkopf1 (DKK1) and receptor activator of nuclear factor-κB ligand (RANKL) were determined by magnetic luminex assays. Flow cytometry was used to detect the RANKL level on peripheral blood T cells.
  
  
  RESULTS
  Compared with healthy controls, the levels of OC-N-MID and CTX in the sera of the RA patients showed no significant difference. Level of RANKL in the sera of the RA patients was higher than that in the controls, while level of DKK1 was lower. The level of RANKL on peripheral blood T cells increased in the RA patients, especially on CD3(+) T cells.
  
  
  CONCLUSIONS
  In RA patients, level of RANKL on T cells is elevated, and the serum level of RANKL increases, but DKK1 decreases.
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Chengdu 610041, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Chengdu 610041, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Chengdu 610041, China.
authors.forename: Zhuochun
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Chengdu 610041, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Niu
authors.affiliation: West China Clinical Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Fengyu
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Chengdu 610041, China.
authors.forename: Junlong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Chengdu 610041, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, West China Hospital, Chengdu 610041, China. *Corresponding author, E-mail: WangLL87@126.com.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D053245:RANK Ligand / Q000097:blood*; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: C501126:DKK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D053245:RANK Ligand