title: Effects of aspirin-triggered resolvin D1 on peripheral blood mononuclear cells from patients with Chagas' heart disease.
abstract: Chagas disease is caused by Trypanosoma cruzi (T. cruzi). In some patients with Chagas disease, symptoms progress to chronic chagasic cardiomyopathy. Endogenously, inflammation is resolved in the presence of lipid mediators such as aspirin-triggered RvD1 (AT-RvD1) which has anti-inflammatory and pro-resolution effects. Here, we demonstrated, for the first time, the effects of AT-RvD1 on T. cruzi antigen-stimulated peripheral blood mononuclear cells (PBMCs) from patients with Chagas heart disease. The levels of IFN-γ, TNF-α, IL-10, and IL-13 increased in PBMCs from cardiac-form Chagas patients in stage B1 (patients with fewer heart abnormalities) stimulated with T. cruzi antigen compared to those in non-stimulated PBMCs. AT-RvD1 reduced the IFN-γ concentrations in PBMCs from patients with Chagas disease stimulated with T. cruzi antigen compared to stimulated with T. cruzi antigen cells. AT-RvD1 treatment resulted in no observable changes in TNF-α, IL-10, and IL-13 levels. AT-RvD1 significantly decreased the percentage of necrotic cells and caused a significant reduction in the proliferation rate of T. cruzi antigen-stimulated PBMCs from patients with Chagas disease. These findings demonstrate that AT-RvD1 modulates the immune response in Chagas disease patients and might have potential to be used as an alternative approach for slowing the development of further heart damage.
authors.affiliation: Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Rua Vigário Carlos 162, Uberaba, Minas Gerais 38025-350, Brazil. Electronic address: haline_04@hotmail.com.
authors.forename: Haline
authors.initials: H
authors.lastname: Ogata
authors.affiliation: Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Rua Vigário Carlos 162, Uberaba, Minas Gerais 38025-350, Brazil. Electronic address: maxellemartins@hotmail.com.
authors.forename: Maxelle Martins
authors.initials: MM
authors.lastname: Teixeira
authors.affiliation: Infectious Disease Division, Internal Medicine Department, Federal University of Triangulo Mineiro, Rua Frei Paulino, 30, Uberaba, Minas Gerais 38025-180, Brazil. Electronic address: rc.sousa@terra.com.br.
authors.forename: Rodrigo Cunha de
authors.initials: RC
authors.lastname: Sousa
authors.affiliation: Infectious Disease Division, Internal Medicine Department, Federal University of Triangulo Mineiro, Rua Frei Paulino, 30, Uberaba, Minas Gerais 38025-180, Brazil. Electronic address: marcosuftm@gmail.com.
authors.forename: Marcos Vinícius da
authors.initials: MV
authors.lastname: Silva
authors.affiliation: Infectious Disease Division, Internal Medicine Department, Federal University of Triangulo Mineiro, Rua Frei Paulino, 30, Uberaba, Minas Gerais 38025-180, Brazil. Electronic address: dalmo@mednet.com.br.
authors.forename: Dalmo
authors.initials: D
authors.lastname: Correia
authors.affiliation: Infectious Disease Division, Internal Medicine Department, Federal University of Triangulo Mineiro, Rua Frei Paulino, 30, Uberaba, Minas Gerais 38025-180, Brazil. Electronic address: vrodrigues@mednet.com.br.
authors.forename: Virmondes
authors.initials: V
authors.lastname: Rodrigues Junior
authors.affiliation: Brigham and Women's Hospital, 77 Ave. Louis Pasteur, HIM 807, Boston, MA 02115, United States. Electronic address: blevy@partners.org.
authors.forename: Bruce David
authors.initials: BD
authors.lastname: Levy
authors.affiliation: Institute of Health Sciences, Department of Clinical Medicine, Laboratory of Experimental Immunopharmacology, Federal University of Triangulo Mineiro, Rua Vigário Carlos 162, Uberaba, Minas Gerais 38025-350, Brazil. Electronic address: alexprogerio@yahoo.com.br.
authors.forename: Alexandre de Paula
authors.initials: Ade P
authors.lastname: Rogério
mesh_terms: D000293:Adolescent; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D001241:Aspirin / Q000494:pharmacology* / Q000627:therapeutic use; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D002598:Chagas Cardiomyopathy / Q000097:blood* / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009336:Necrosis / Q000473:pathology; D055815:Young Adult
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C518399:resolvin D1; D004281:Docosahexaenoic Acids; D007371:Interferon-gamma; D001241:Aspirin
keywords: AT-RvD1; Chagas disease; PBMC; T. cruzi antigens