title: Double-wavelength overlapping resonance Rayleigh scattering technique for the simultaneous quantitative analysis of three β-adrenergic blockade.
abstract: Four simple and accurate spectrophotometric methods were proposed for the simultaneous determination of three β-adrenergic blockade, e.g. atenolol, metoprolol and propranolol. The methods were based on the reaction of the three drugs with erythrosine B (EB) in a Britton-Robinson buffer solution at pH4.6. EB could combine with the drugs to form three ion-association complexes, which resulted in the resonance Rayleigh scattering (RRS) intensity that is enhanced significantly with new RRS peaks that appeared at 337 nm and 370 nm, respectively. In addition, the fluorescence intensity of EB was also quenched. The enhanced scattering intensities of the two peaks and the fluorescence quenched intensity of EB were proportional to the concentrations of the drugs, respectively. What is more, the RRS intensity overlapped with the double-wavelength of 337 nm and 370 nm (so short for DW-RRS) was also proportional to the drugs concentrations. So, a new method with highly sensitive for simultaneous determination of three bisoprolol drugs was established. Finally, the optimum reaction conditions, influencing factors and spectral enhanced mechanism were investigated. The new DW-RRS method has been applied to simultaneously detect the three β-blockers in fresh serum with satisfactory results.
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Xuanping
authors.initials: X
authors.lastname: Tan
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China; School of Chemistry and Environmental Engineering, Chongqing Three Gorges University, Chongqing 404000, China; School of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China. Electronic address: flyjd6400@163.com.
authors.forename: Jidong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Chongqing Medical and Health school, Fuling, Chongqing 408100, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yizhong
authors.initials: Y
authors.lastname: Shen
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis / Q000097:blood*; D001262:Atenolol / Q000032:analysis / Q000097:blood*; D004923:Erythrosine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008027:Light; D057230:Limit of Detection; D008790:Metoprolol / Q000032:analysis / Q000097:blood*; D011433:Propranolol / Q000032:analysis / Q000097:blood*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000319:Adrenergic beta-Antagonists; D005456:Fluorescent Dyes; D001262:Atenolol; D011433:Propranolol; D008790:Metoprolol; D004923:Erythrosine
keywords: Bisoprolol drugs; Double-wavelength overlapping; Fluorescence quenched; Resonance Rayleigh scattering; Simultaneous determine; Spectrophotometric method