title: In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers.
abstract: The aim of this study was to evaluate whether the modification in the silver component is capable of providing GuttaFlow 2 with antibacterial activity against Enterococcus faecalis compared with epoxy resin-based (AH Plus) and zinc oxide and eugenol-based (Endofill) sealers. The antibacterial activity was evaluated using a reference strain of E. faecalis (ATCC 29212). Freshly mixed sealers were subjected to the agar diffusion test (ADT), while the direct contact test (DCT) was performed after materials setting. ADT results were obtained through measurements, in millimeters, of the inhibition zones promoted by the materials, using a digital caliper. In DCT, values of CFU/mL promoted by the three sealers were compared in three experimental periods (1 min, 1 h, and 24 h). The data were analyzed using Kruskal-Wallis and Dunn post-hoc tests (p < 0.05). In both ADT and DCT, GuttaFlow 2 presented no effect against E. faecalis, while Endofill and AH Plus showed similar inhibition zones. Endofill was the only material capable of reducing bacterial growth in DCT. In conclusion, modifications in the silver particle of GuttaFlow 2 did not result in a sealer with antibacterial effect against E. faecalis.
authors.affiliation: Department of Clinicals, School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Marcela
authors.initials: M
authors.lastname: Wainstein
authors.affiliation: Department of Clinicals, School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Renata Dornelles
authors.initials: RD
authors.lastname: Morgental
authors.affiliation: Department of Clinicals, School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Silvana Beltrami Gonçalves
authors.initials: SB
authors.lastname: Waltrick
authors.affiliation: Laboratory of Immunology and Microbiology, School of Biosciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Sílvia Dias
authors.initials: SD
authors.lastname: Oliveira
authors.affiliation: Department of Clinicals, School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Fabiana Vieira
authors.initials: FV
authors.lastname: Vier-Pelisser
authors.affiliation: Department of Clinicals, School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: José Antonio Poli
authors.initials: JA
authors.lastname: Figueiredo
authors.affiliation: Department of Dentistry/Endodontics, Warwick Medical School, University of Warwick, Warwick, United Kingdom.
authors.forename: Liviu
authors.initials: L
authors.lastname: Steier
authors.affiliation: Laboratory of Immunology and Microbiology, School of Biosciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Cauana Oliva
authors.initials: CO
authors.lastname: Tavares
authors.affiliation: Laboratory of Immunology and Microbiology, School of Biosciences, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Roberta Kochenborger
authors.initials: RK
authors.lastname: Scarparo
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology*; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D004853:Epoxy Resins / Q000737:chemistry / Q000494:pharmacology*; D006171:Gutta-Percha / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D010316:Particle Size; D015203:Reproducibility of Results; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D012828:Silicones / Q000737:chemistry / Q000494:pharmacology; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D004853:Epoxy Resins; C515718:GuttaFlow; D012387:Root Canal Filling Materials; D012828:Silicones; C534916:epoxy resin-based root canal sealer; C074417:Endo-Fill; D012834:Silver; D006171:Gutta-Percha