title: Muscle and liver-specific alterations in lipid and acylcarnitine metabolism after a single bout of exercise in mice.
abstract: Intracellular lipid pools are highly dynamic and tissue-specific. Physical exercise is a strong physiologic modulator of lipid metabolism, but most studies focus on changes induced by long-term training. To assess the acute effects of endurance exercise, mice were subjected to one hour of treadmill running, and (13)C16-palmitate was applied to trace fatty acid incorporation in soleus and gastrocnemius muscle and liver. The amounts of carnitine, FFA, lysophospholipids and diacylglycerol and the post-exercise increase in acetylcarnitine were pronouncedly higher in soleus than in gastrocnemius. In the liver, exercise increased the content of lysophospholipids, plasmalogens and carnitine as well as transcript levels of the carnitine transporter. (13)C16-palmitate was detectable in several lipid and acylcarnitine species, with pronounced levels of tracer-derived palmitoylcarnitine in both muscles and a strikingly high incorporation into triacylglycerol and phosphatidylcholine in the liver. These data illustrate the high lipid storing activity of the liver immediately after exercise whereas in muscle, fatty acids are directed towards oxidation. The observed muscle-specific differences accentuate the need for single-muscle analyses as well as careful consideration of the particular muscle employed when studying lipid metabolism in mice. In addition, our results reveal that lysophospholipids and plasmalogens, potential lipid signalling molecules, are acutely regulated by physical exercise.
authors.affiliation: Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Hoene
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany.
authors.forename: Heike
authors.initials: H
authors.lastname: Runge
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany.|Department of Molecular Diabetology, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Centre Munich at the University of Tuebingen, Tuebingen, Germany.|German Center for Diabetes Research (DZD), Tuebingen, Germany.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Häring
authors.affiliation: Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany.|Department of Molecular Diabetology, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Centre Munich at the University of Tuebingen, Tuebingen, Germany.|German Center for Diabetes Research (DZD), Tuebingen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Lehmann
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Division of Clinical Chemistry and Pathobiochemistry, Department of Diagnostic Laboratory Medicine, University Hospital Tuebingen, Tuebingen, Germany.|Department of Molecular Diabetology, Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Centre Munich at the University of Tuebingen, Tuebingen, Germany.|German Center for Diabetes Research (DZD), Tuebingen, Germany.
authors.forename: Cora
authors.initials: C
authors.lastname: Weigert
mesh_terms: D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives* / Q000378:metabolism; D006019:Glycolysis; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008246:Lysophospholipids / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D009928:Organ Specificity; D010084:Oxidation-Reduction; D010805:Physical Conditioning, Animal; D010955:Plasmalogens / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D010955:Plasmalogens; C116917:acylcarnitine; D002331:Carnitine