title: Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets.
abstract: Chemokine receptors are differentially expressed on leukocyte subpopulations dictating their ability to migrate both in physiological and pathological conditions. Their expression is modulated during leukocyte differentiation and maturation and they can be used as markers to identify and characterize the frequency and the activation state of leukocytes present in a tissue. Here, we will describe flow cytometry approaches to detect chemokine receptors identifying subpopulations of circulating monocytes and neutrophils.
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine, Università degli Studi di Milano, Milano, Italy.
authors.forename: Ornella
authors.initials: O
authors.lastname: Bonavita
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine, Università degli Studi di Milano, Milano, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Massara
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Italy.
authors.forename: Achille
authors.initials: A
authors.lastname: Anselmo
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Somma
authors.affiliation: Universitätsklinikum Regensburg, Regensburg, Germany.
authors.forename: Hilke
authors.initials: H
authors.lastname: Brühl
authors.affiliation: Universitätsklinikum Regensburg, Regensburg, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Mack
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine, Università degli Studi di Milano, Milano, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Locati
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, Italy; Department of Biomedical Sciences, Humanitas University, Rozzano, Italy. Electronic address: raffaella.bonecchi@humanitasresearch.it.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Bonecchi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D019707:Receptors, Chemokine / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D019707:Receptors, Chemokine
keywords: Chemokine receptors; Chemokines; Flow cytometry; Monocytes; Neutrophils