title: Epb41l3 suppresses esophageal squamous cell carcinoma invasion and inhibits MMP2 and MMP9 expression.
abstract: EPB41L3 may play a role as a metastasis suppressor by supporting regular arrangements of actin stress fibres and alleviating the increase in cell motility associated with enhanced metastatic potential. Downregulation of epb41l3 has been observed in many cancers, but the role of this gene in esophageal squamous cell carcinoma (ESCC) remains unclear. Our study aimed to determine the effect of epb41l3 on ESCC cell migration and invasion. We investigated epb41l3 protein expression in tumour and non-tumour tissues by immunohistochemical staining. Expression in the non-neoplastic human esophageal cell line Het-1a and four ESCC cell lines - Kyse150, Kyse510, Kyse450 and Caes17 - was assessed by quantitative Polymerase Chain Reaction (qPCR) and Western blotting. Furthermore, an EPB41L3 overexpression plasmid and EPB41L3-specific small interfering RNA were used to upregulate EPB41L3 expression in Kyse150 cells and to downregulate EPB41L3 expression in Kyse450 cells, respectively. Cell migration and invasion were evaluated by wound healing and transwell assays, respectively. The expression levels of p-AKT, matrix metalloproteinase (MMP)2 and MMP9 were evaluated. Expression of epb41l3 was significantly lower in tumour tissues than in non-tumour tissues and in ESCC cell lines compared with the Het-1a cell line. Kyse450 and Caes17 cells exhibited higher expression of epb41l3 than Kyse150 and Kyse510 cells. Overexpressing epb41l3 decreased Kyse150 cell migration and invasion, whereas EPB41L3-specific small interfering RNA silencing increased these functions in Kyse450 cells. Furthermore, overexpressing epb41l3 led to downregulation of MMP2 and MMP9 in Kyse150 and Kyse510 cells. Our findings reveal that EPB41L3 suppresses tumour cell invasion and inhibits MMP2 and MMP9 expression in ESCC cells.
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Jun-Peng
authors.initials: JP
authors.lastname: Huang
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Xu Feng
authors.initials: XF
authors.lastname: Li
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Xiong
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Zhao-Jing
authors.initials: ZJ
authors.lastname: Jiang
authors.affiliation: Oncology Center, Yuhuangding Hospital, Medical College, Qingdao University, Yantai, Shandong, China.
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Song
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Ji-Qiang
authors.initials: JQ
authors.lastname: Li
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zheng
authors.affiliation: Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Ji-Ren
authors.initials: JR
authors.lastname: Zhang
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002465:Cell Movement; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C116887:EPB41L3 protein, human; D008840:Microfilament Proteins; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: ESCC; Epb41l3; MMP2; MMP9; invasion