title: Synthesis, Structure, Properties, and Bioimaging of a Fluorescent Nitrogen-Linked Bisbenzothiadiazole.
abstract: This paper describes the synthesis, structure, photophysical properties, and bioimaging application of a novel 2,1,3-benzothiadiazole (BTD)-based rationally designed fluorophore. The capability of undergoing efficient stabilizing processes from the excited state allowed the novel BTD derivative to be used as a stable probe for bioimaging applications. No notable photobleaching effect or degradation could be observed during the experimental time period. Before the synthesis, the molecular architecture of the novel BTD derivative was evaluated by means of DFT calculations to validate the chosen design. Single-crystal X-ray analysis revealed the nearly flat characteristics of the structure in a syn conformation. The fluorophore was successfully tested as a live-cell-imaging probe and efficiently stained MCF-7 breast cancer cell lineages.
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: Alberto A R
authors.initials: AA
authors.lastname: Mota
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: José R
authors.initials: JR
authors.lastname: Corrêa
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: Pedro H P R
authors.initials: PH
authors.lastname: Carvalho
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: Núbia M P
authors.initials: NM
authors.lastname: de Sousa
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: Heibbe C B
authors.initials: HC
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: Claudia C
authors.initials: CC
authors.lastname: Gatto
authors.affiliation: Institute of Physics, University of Brasilia (IF-UnB) , Campus Universitario Darcy Ribeiro, CEP, Brasilia, DF 70919-970, Brazil.
authors.forename: Demétrio A
authors.initials: DA
authors.lastname: da Silva Filho
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: Aline L
authors.initials: AL
authors.lastname: de Oliveira
authors.affiliation: Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB) , Campus Universitário Darcy Ribeiro, CEP, P.O. Box 4478,Brasilia, DF 70904-970, Brazil.
authors.forename: Brenno A D
authors.initials: BA
authors.lastname: Neto
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D009584:Nitrogen / Q000737:chemistry*; D011789:Quantum Theory; D013830:Thiadiazoles / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; D009584:Nitrogen