title: Desacetyl bisacodyl-induced epithelial Cl(-) secretion in rat colon and rectum.
abstract: The purpose of this study was to clarify the mode of desacetyl bisacodyl (DAB)-induced secretory action in intestinal tissues using an Ussing chamber assay. DAB is the active metabolite of the laxative bisacodyl. In mucosal-submucosal preparations, mucosal application of DAB induced a transient decrease followed by subsequent increases in short-circuit current and tissue conductance in a concentration-dependent manner. DAB-induced responses occurred from the middle colon to the rectal segment but not in the proximal colon. Moreover, these responses were not observed under chloride (Cl(-))-free conditions or in the presence of DAB on the serosal side of the mucosalsubmucosal specimens. Treatment with tetrodotoxin had no effect on the DAB-induced responses; however, mucosal treatment with a COX inhibitor piroxicam resulted in the elimination of responses. These results suggest that DAB may contribute to the laxative action by inducing Cl(-) secretion which is associated with the COX signaling pathway. This study also demonstrated that the DAB target molecule is present on the mucosal side from the middle colon to the rectal segment.
authors.affiliation: Laboratory of Physiology, Department of Environmental and Life Sciences/Graduate School of Nutritional and Environmental Sciences, University of Shizuoka.
authors.forename: Takuya
authors.initials: T
authors.lastname: Fujita
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Karaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Tateoka
authors.forename: Atsukazu
authors.initials: A
authors.lastname: Kuwahara
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001726:Bisacodyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D003106:Colon / Q000378:metabolism* / Q000502:physiology; D004573:Electrolytes / Q000378:metabolism; D055724:Electrophysiological Phenomena / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051381:Rats; D012007:Rectum / Q000378:metabolism* / Q000502:physiology
chemical_list: D002712:Chlorides; D004573:Electrolytes; D001726:Bisacodyl; C001659:dihydroxydiphenyl-pyridyl methane