title: Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae).
abstract: This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA3 or 0.2 µM NAA, sucrose at 15 or 30 g L-1, in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA3 and 15 g L-1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L-1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: Resende
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: V F
authors.initials: VF
authors.lastname: Braga
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: P F
authors.initials: PF
authors.lastname: Pereira
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: C J
authors.initials: CJ
authors.lastname: Silva
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: V F
authors.initials: VF
authors.lastname: Vale
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: R E
authors.initials: RE
authors.lastname: Bianchetti
authors.affiliation: Instituto de Pesquisa do Jardim Botânico do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: R C
authors.initials: RC
authors.lastname: Forzza
authors.affiliation: Centro de Ciências Biológicas, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Ribeiro
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: P H P
authors.initials: PH
authors.lastname: Peixoto
mesh_terms: D000064:Acclimatization; D031229:Bromeliaceae / Q000254:growth & development / Q000502:physiology*; D056727:Endangered Species; D010084:Oxidation-Reduction; D010788:Photosynthesis; D011392:Proline / Q000378:metabolism*
chemical_list: D011392:Proline