title: Specific cytoarchitectureal changes in hippocampal subareas in daDREAM mice.
abstract: BACKGROUND
  Transcriptional repressor DREAM (downstream regulatory element antagonist modulator) is a Ca(2+)-binding protein that regulates Ca(2+) homeostasis through gene regulation and protein-protein interactions. It has been shown that a dominant active form (daDREAM) is implicated in learning-related synaptic plasticity such as LTP and LTD in the hippocampus. Neuronal spines are reported to play important roles in plasticity and memory. However, the possible role of DREAM in spine plasticity has not been reported.
  
  
  RESULTS
  Here we show that potentiating DREAM activity, by overexpressing daDREAM, reduced dendritic basal arborization and spine density in CA1 pyramidal neurons and increased spine density in dendrites in dentate gyrus granule cells. These microanatomical changes are accompanied by significant modifications in the expression of specific genes encoding the cytoskeletal proteins Arc, Formin 1 and Gelsolin in daDREAM hippocampus.
  
  
  CONCLUSIONS
  Our results strongly suggest that DREAM plays an important role in structural plasticity in the hippocampus.
authors.affiliation: Spanish Network for Biomedical Research in Neurodegenerative Diseases, CIBERNED, Madrid, Spain. bmellstr@cnb.csic.es.|National Biotechnology Center. CSIC, Darwin, 3. E-28049, Madrid, Spain. bmellstr@cnb.csic.es.
authors.forename: Britt
authors.initials: B
authors.lastname: Mellström
authors.affiliation: Spanish Network for Biomedical Research in Neurodegenerative Diseases, CIBERNED, Madrid, Spain. kastanauskaite@gmail.com.|Cajal Institute, CSIC Madrid, Av Dr. Arce,37 E-28006, Madrid, Spain. kastanauskaite@gmail.com.|Biomedical Technology Center, Politecnica University Madrid, Madrid, Spain. kastanauskaite@gmail.com.
authors.forename: Asta
authors.initials: A
authors.lastname: Kastanauskaite
authors.affiliation: Cajal Institute, CSIC Madrid, Av Dr. Arce,37 E-28006, Madrid, Spain. s.knafo@ikerbasque.org.|Present address: IkerBasque Basque Foundation for Science and BioCruces, Health Research Institute, Bizkaia, Spain. s.knafo@ikerbasque.org.
authors.forename: Shira
authors.initials: S
authors.lastname: Knafo
authors.affiliation: Spanish Network for Biomedical Research in Neurodegenerative Diseases, CIBERNED, Madrid, Spain. pgperez@cnb.csic.es.|National Biotechnology Center. CSIC, Darwin, 3. E-28049, Madrid, Spain. pgperez@cnb.csic.es.
authors.forename: Paz
authors.initials: P
authors.lastname: Gonzalez
authors.affiliation: Spanish Network for Biomedical Research in Neurodegenerative Diseases, CIBERNED, Madrid, Spain. jmdopazo@cnb.csic.es.|National Biotechnology Center. CSIC, Darwin, 3. E-28049, Madrid, Spain. jmdopazo@cnb.csic.es.
authors.forename: Xose M
authors.initials: XM
authors.lastname: Dopazo
authors.affiliation: Neuronal Networks Group, School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, UK. anaruiznuno@yahoo.es.
authors.forename: Ana
authors.initials: A
authors.lastname: Ruiz-Nuño
authors.affiliation: Neuronal Networks Group, School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, UK. J.G.R.Jefferys@bham.ac.uk.
authors.forename: John G R
authors.initials: JG
authors.lastname: Jefferys
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada. minzhuo10@gmail.com.|Center for Neuron and Disease, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, China. minzhuo10@gmail.com.
authors.forename: Min
authors.initials: M
authors.lastname: Zhuo
authors.affiliation: MRC National Institutes for Medical Research, Mill Hill, London, UK. tim.bliss@crick.ac.uk.
authors.forename: Tim V P
authors.initials: TV
authors.lastname: Bliss
authors.affiliation: Spanish Network for Biomedical Research in Neurodegenerative Diseases, CIBERNED, Madrid, Spain. naranjo@cnb.csic.es.|National Biotechnology Center. CSIC, Darwin, 3. E-28049, Madrid, Spain. naranjo@cnb.csic.es.
authors.forename: Jose R
authors.initials: JR
authors.lastname: Naranjo
authors.affiliation: Spanish Network for Biomedical Research in Neurodegenerative Diseases, CIBERNED, Madrid, Spain. defelipe@cajal.csic.es.|Cajal Institute, CSIC Madrid, Av Dr. Arce,37 E-28006, Madrid, Spain. defelipe@cajal.csic.es.|Biomedical Technology Center, Politecnica University Madrid, Madrid, Spain. defelipe@cajal.csic.es.
authors.forename: Javier
authors.initials: J
authors.lastname: DeFelipe
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D049229:Dendritic Spines / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism*; D007546:Isoquinolines / Q000378:metabolism; D051605:Kv Channel-Interacting Proteins / Q000378:metabolism*; D008822:Mice, Transgenic
chemical_list: D007546:Isoquinolines; D051605:Kv Channel-Interacting Proteins; C017475:lucifer yellow