title: Glycosidase- and β-lactamase-like activity of dinuclear copper(II) patellamide complexes.
abstract: Prochloron, a blue-green algae belonging to ancient prokaryotes, produces, like other cyanobacteria, cyclic pseudo-peptides, which are also found in its obligate symbiont ascidiae (Lissoclinum patellum). Although research has focused for some time on the putative metabolic function of these cyclic peptides, to date it is still not understood. Their role might be connected to the increased concentrations of divalent metal ions, especially Cu(II), found in ascidiae. Dinuclear copper(II) complexes of cyclic pseudo-peptides revealed a broad hydrolytic capacity, including carboanhydrase and phosphatase activity. This study reports their β-lactamase as well as α- and β-glycosidase activity with kcat=(11.34±0.91)ˑ10(-4)s(-1) for β-lactamase, kcat=(1.55±0.13)ˑ10(-4)s(-1) for α-glycosidase and kcat=(1.22±0.09)ˑ10(-4)s(-1) for β-glycosidase activity.
authors.affiliation: University of Heidelberg, Institute of Inorganic Chemistry and Interdisciplinary Center for Scientific Computing, Im Neuenheimer Feld 270, D-69120 Heidelberg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Comba
authors.affiliation: University of Heidelberg, Institute of Inorganic Chemistry and Interdisciplinary Center for Scientific Computing, Im Neuenheimer Feld 270, D-69120 Heidelberg, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Eisenschmidt
authors.affiliation: University of Heidelberg, Institute of Inorganic Chemistry and Interdisciplinary Center for Scientific Computing, Im Neuenheimer Feld 270, D-69120 Heidelberg, Germany.
authors.forename: Nora
authors.initials: N
authors.lastname: Kipper
authors.affiliation: University of Heidelberg, Institute of Inorganic Chemistry and Interdisciplinary Center for Scientific Computing, Im Neuenheimer Feld 270, D-69120 Heidelberg, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Schießl
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D000458:Cyanobacteria / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D001618:beta-Lactamases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D010456:Peptides, Cyclic; D003300:Copper; D006026:Glycoside Hydrolases; D001618:beta-Lactamases
keywords: Ascidian; Copper(II) complexes; Cyclic peptides; Glycosidase; Lactamase; Prochloron