title: Inhibitory effects of Enteromorpha linza polysaccharide on micronucleus of Allium sativum root cells.
abstract: In this study, the antimutagenic function of the polysaccharide from Enteromorpha linza with the micronucleus test of Allium sativum root cells induced by sulfur dioxide and ultraviolet was studied. The concentration-effect relation of the two inducers was firstly evaluated. The results showed that an increase of genotoxicity damage was demonstrated and micronuclei frequency induced by sulfur dioxide and ultraviolet displayed dose dependent increases. All the doses of polysaccharide did affect the micronuclei frequency formation compared with the negative control. And also, the significant increase in inhibition rate of micronuclei frequency was observed with the increase of the dose of polysaccharide. It was showed maximum inhibition of micronuclei frequency cells (71.74% and 66.70%) at a concentration of 200g/mL in three experiments. The low molecular weight polysaccharide showed higher inhibition rate than raw polysaccharide at the higher concentration (50g/mL) in the absence of sulfur dioxide and ultraviolet. It was confirmed to be a good mutant inhibitor.
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China. Electronic address: zhzs@hutc.zj.cn.
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China.
authors.forename: Jingfen
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Medicine, Huzhou University, Huzhou 313000, China.
authors.forename: Chongbin
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D005737:Garlic / Q000166:cytology*; D015162:Micronucleus Tests; D018517:Plant Roots / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D011134:Polysaccharides / Q000494:pharmacology*; D013458:Sulfur Dioxide / Q000633:toxicity; D014466:Ultraviolet Rays / Q000009:adverse effects; D044445:Ulva / Q000737:chemistry*
chemical_list: D016587:Antimutagenic Agents; D011134:Polysaccharides; D013458:Sulfur Dioxide
keywords: Antimutation; Enteromorpha linza; Polysaccharide