title: Electron microscopy of myelin: Structure preservation by high-pressure freezing.
abstract: Electron microscopic visualization of nervous tissue morphology is crucial when aiming to understand the biogenesis and structure of myelin in healthy and pathological conditions. However, accurate interpretation of electron micrographs requires excellent tissue preservation. In this short review we discuss the recent utilization of tissue fixation by high-pressure freezing and freeze-substitution, which now supplements aldehyde fixation in the preparation of samples for electron microscopy of myelin. Cryofixation has proven well suited to yield both, improved contrast and excellent preservation of structural detail of the axon/myelin-unit in healthy and mutant mice and can also be applied to other model organisms, including aquatic species. This article is part of a Special Issue entitled SI: Myelin Evolution.
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany; Center for Nanoscale Microscopy and Molecular Physiology of the Brain, 37075 Göttingen, Germany. Electronic address: Moebius@em.mpg.de.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Möbius
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany. Electronic address: Nave@em.mpg.de.
authors.forename: Klaus-Armin
authors.initials: KA
authors.lastname: Nave
authors.affiliation: Department of Neurogenetics, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany. Electronic address: Hauke@em.mpg.de.
authors.forename: Hauke B
authors.initials: HB
authors.lastname: Werner
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure*
keywords: Demyelination and remyelination; Earthworm lumbricus terrestris; Leukodystrophy; Mouse; Neuropathy; Oligodendrocyte; Schwann cell; Transmission electron microscopy; Xenopus; Zebrafish