title: Pravastatin induces cell cycle arrest and decreased production of VEGF and bFGF in multiple myeloma cell line.
abstract: Multiple myeloma (MM) is a B cell bone marrow neoplasia characterized by inflammation with an intense secretion of growth factors that promote tumor growth, cell survival, migration and invasion. The aim of this study was to evaluate the effects of pravastatin, a drug used to reduce cholesterol, in a MM cell line.Cell cycle and viability were determinate by Trypan Blue and Propidium Iodide. IL6, VEGF, bFGF and TGFβ were quantified by ELISA and qRT-PCR including here de HMG CoA reductase. It was observed reduction of cell viability, increase of cells in G0/G1 phase of the cell cycle and reducing the factors VEGF and bFGF without influence on 3-Methyl-Glutaryl Coenzyme A reductase expression.The results demonstrated that pravastatin induces cell cycle arrest in G0/G1 and decreased production of growth factors in Multiple Myeloma cell line.
authors.affiliation: Laboratório Multidisciplinar de Ciências Biológicas e da Saúde, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: P J J
authors.initials: PJ
authors.lastname: Trojan
authors.affiliation: Laboratório Multidisciplinar de Ciências Biológicas e da Saúde, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Bohatch-Junior
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: Otuki
authors.affiliation: Unit Cytokines and Inflammation, Department Infection and Epidemiology Institut Pasteur, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Souza-Fonseca-Guimarães
authors.affiliation: Departamento de Biologia Molecular, Estrutural e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: P V
authors.initials: PV
authors.lastname: Svidnicki
authors.affiliation: Departamento de Biologia Molecular, Estrutural e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: V
authors.initials: V
authors.lastname: Nogaroto
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Fernandes
authors.affiliation: Departamento de Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: E A
authors.initials: EA
authors.lastname: Krum
authors.affiliation: Laboratório Multidisciplinar de Ciências Biológicas e da Saúde, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
authors.forename: G M
authors.initials: GM
authors.lastname: Favero
mesh_terms: D000924:Anticholesteremic Agents / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D009101:Multiple Myeloma / Q000378:metabolism*; D017035:Pravastatin / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D000924:Anticholesteremic Agents; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C108111:fibroblast growth factor 13; D005346:Fibroblast Growth Factors; D002784:Cholesterol; D017035:Pravastatin