title: Molecular screening for Anaplasmataceae in ticks and tsetse flies from Ethiopia.
abstract: Hard ticks and tsetse flies are regarded as the most important vectors of disease agents in Sub-Saharan Africa. With the aim of screening these blood-sucking arthropods for vector-borne pathogens belonging to the family Anaplasmataceae in South-Western Ethiopia, four species of tsetse flies (collected by traps) and seven species of ixodid ticks (removed from cattle) were molecularly analysed. DNA was extracted from 296 individual ticks and from 162 individuals or pools of tsetse flies. Besides known vector-pathogen associations, in Amblyomma cohaerens ticks sequences of Anaplasma marginale and A. phagocytophilum were detected, the latter for the first time in any ticks from cattle in Africa. In addition, part of the gltA gene of Ehrlichia ruminantium was successfully amplified from tsetse flies (Glossina pallidipes). First-time identification of sequences of the above pathogens in certain tick or tsetse fly species may serve as the basis of further epidemiological and transmission studies.
authors.affiliation: Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University , István u. 2, H-1078 Budapest , Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Hornok
authors.affiliation: National Animal Health Diagnostic and Investigation Center (NAHDIC) , Department of Parasitology, Sebeta , Ethiopia.
authors.forename: Getachew
authors.initials: G
authors.lastname: Abichu
authors.affiliation: Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University , István u. 2, H-1078 Budapest , Hungary.
authors.forename: Nóra
authors.initials: N
authors.lastname: Takács
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences , Budapest , Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Gyuranecz
authors.affiliation: Department of Parasitology and Zoology, Faculty of Veterinary Science, Szent István University , István u. 2, H-1078 Budapest , Hungary.
authors.forename: Róbert
authors.initials: R
authors.lastname: Farkas
authors.affiliation: SaBio , Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM, Ciudad Real , Spain.
authors.forename: Isabel G
authors.initials: IG
authors.lastname: Fernández De Mera
authors.affiliation: SaBio , Instituto de Investigación en Recursos Cinegéticos IREC, CSIC-UCLM-JCCM, Ciudad Real , Spain.|Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University , Stillwater , USA.
authors.forename: José
authors.initials: J
authors.lastname: De La Fuente
mesh_terms: D000710:Anaplasmataceae / Q000302:isolation & purification*; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000469:parasitology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004199:Disease Vectors; D005002:Ethiopia; D013984:Tick Infestations / Q000469:parasitology / Q000662:veterinary; D013987:Ticks / Q000382:microbiology*; D014370:Tsetse Flies / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: A. marginale; Amblyomma; Anaplasma phagocytophilum; Ehrlichia ruminantium; Glossina; ticks; tsetse flies