title: A surface acoustic wave (SAW)-enhanced grating-coupling phase-interrogation surface plasmon resonance (SPR) microfluidic biosensor.
abstract: A surface acoustic wave (SAW)-enhanced, surface plasmon resonance (SPR) microfluidic biosensor in which SAW-induced mixing and phase-interrogation grating-coupling SPR are combined in a single lithium niobate lab-on-a-chip is demonstrated. Thiol-polyethylene glycol adsorption and avidin/biotin binding kinetics were monitored by exploiting the high sensitivity of grating-coupling SPR under azimuthal control. A time saturation binding kinetics reduction of 82% and 24% for polyethylene and avidin adsorption was obtained, respectively, due to the fluid mixing enhancement by means of the SAW-generated chaotic advection. These results represent the first implementation of a nanostructured SAW-SPR microfluidic biochip capable of significantly improving the molecule binding kinetics on a single, portable device. In addition, the biochip here proposed is suitable for a great variety of biosensing applications.
authors.affiliation: CNR-IOM, Area Science Park, S.S. 14, km 163.5, 34149, Basovizza (TS), Italy.
authors.forename: A
authors.initials: A
authors.lastname: Sonato
authors.affiliation: NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy. marco.cecchini@nano.cnr.it and Center for Nanotechnology Innovation@NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Agostini
authors.affiliation: Department of Physics and Astronomy, University of Padova, Via Marzolo 8, 35131 Padova, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ruffato
authors.affiliation: Department of Physics and Astronomy, University of Padova, Via Marzolo 8, 35131 Padova, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Gazzola
authors.affiliation: Department of Physics and Astronomy, University of Padova, Via Marzolo 8, 35131 Padova, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Liuni
authors.affiliation: NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy. marco.cecchini@nano.cnr.it.
authors.forename: G
authors.initials: G
authors.lastname: Greco
authors.affiliation: NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy. marco.cecchini@nano.cnr.it and Center for Nanotechnology Innovation@NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, 56127 Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Travagliati
authors.affiliation: NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy. marco.cecchini@nano.cnr.it.
authors.forename: M
authors.initials: M
authors.lastname: Cecchini
authors.affiliation: CNR-IOM, Area Science Park, S.S. 14, km 163.5, 34149, Basovizza (TS), Italy and Department of Physics and Astronomy, University of Padova, Via Marzolo 8, 35131 Padova, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Romanato
mesh_terms: D007700:Kinetics; D056656:Lab-On-A-Chip Devices; D013016:Sound; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties