title: Constitutively expressed Siglec-9 inhibits LPS-induced CCR7, but enhances IL-4-induced CD200R expression in human macrophages.
abstract: Siglecs recognize the sialic acid moiety and regulate various immune responses. In the present study, we compared the expression levels of Siglecs in human monocytes and macrophages using a quantitative real-time reverse transcription-polymerase chain reaction analysis. The differentiation of monocytes into macrophages by macrophage colony-stimulating factor or granulocyte macrophage colony-stimulating factor enhanced the expression of Siglec-7 and Siglec-9. The differentiated macrophages were stimulated by lipopolysaccharide (LPS) plus interferon (IFN)-γ or interleukin (IL)-4. The expression of Siglec-10 was enhanced by IL-4, whereas that of Siglec-7 was reduced by LPS plus IFN-γ. The expression of Siglec-9 was not affected by these stimuli. The knockdown of Siglec-9 enhanced the expression of CCR7 induced by the LPS or the LPS plus IFN-γ stimulation, and decreased the IL-4-induced expression of CD200R. These results suggest that Siglec-9 is one of the main Siglecs in human blood monocytes/macrophages and modulates innate immunity.
authors.affiliation: a Department of Biotechnology , Nagoya University , Nagoya , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Higuchi
authors.affiliation: a Department of Biotechnology , Nagoya University , Nagoya , Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Shoji
authors.affiliation: a Department of Biotechnology , Nagoya University , Nagoya , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
authors.affiliation: a Department of Biotechnology , Nagoya University , Nagoya , Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nishijima
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D064446:Orexin Receptors; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D054400:Receptors, CCR7 / Q000235:genetics / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology*; D063265:Sialic Acid Binding Immunoglobulin-like Lectins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; C515370:CCR7 protein, human; C477337:CD200R1 protein, human; C508598:IL4 protein, human; D008070:Lipopolysaccharides; D064446:Orexin Receptors; D034741:RNA, Small Interfering; D054400:Receptors, CCR7; D011956:Receptors, Cell Surface; C412227:SIGLEC9 protein, human; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D015847:Interleukin-4; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: Siglec; human; lectin; macrophages; sialic acid