title: [Picosecond pulsed electric field inhibits the proliferation and migration, induces G2/M phase arrest and promotes apoptosis in human umbilical venous endothelial cells].
abstract: OBJECTIVE
  To study the impact of intense picosecond pulsed electric field (psPEF) on the biological behaviors of human umbilical venous endothelial cells (HUVECs) in vitro.
  
  
  METHODS
  Intense psPEF with constant parameters (pulse duration, 800 ps; recurrence rate, 3 Hz; the number of pulses, 2000) and three-level electric field strength (200, 300 and 400 KV/cm) were performed on HUVECs. CCK-8 assay was used to detect the cell proliferation of HUVECs treated with psPEF for 2, 4, 6, 8, 10, 12, 14, 16 hours. The effect of psPEF on HUVEC migration was measured by wound healing method and Transwell(TM) assay. The apoptosis and cell cycle were detected by flow cytometry.
  
  
  RESULTS
  The death rate of HUVECs went up with the increase of the amount of pulses, and the maximum inhibitory rate was at 12 hours after the treatment with psPEF. Intense psPEF inhibited dose-dependently the migration of HUVECs. What's more, the treatment with psPEF arrested cell cycle in G2/M phase and increased the apoptosis rate.
  
  
  CONCLUSIONS
  Intense psPEF inhibits the migration and proliferation of HUVECs as well as induces cell apoptosis and arrests the cell cycle in G2/M phase.
authors.affiliation: Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Yutong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China. *Corresponding author, E-mail: 1178236544@qq.com.
authors.forename: Zhengai
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Chenguo
authors.initials: C
authors.lastname: Yao
authors.affiliation: Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Ruizhe
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D004558:Electric Stimulation / Q000379:methods; D005434:Flow Cytometry; D059565:G2 Phase Cell Cycle Checkpoints / Q000502:physiology*; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology*; D006801:Humans; D013997:Time Factors