title: Expression and significance of miR-21 in multiple myeloma patients.
abstract: The aim of the present study is to examine the expression level of peripheral mir-21 in multiple myeloma (MM) patients and to determine its clinical significance. MM patients (30), monoclonal gammopathy of undetermined significance (MGUS) patients (14), and normal controls (20) were recruited to determine the serum level of β2-MG, IgA and IgM, IgG, λ, κ, TP, ALB, Hb, LDH, and Ca(2+). Gene expression of mir-21 was quantified by SYBR green real-time fluorescent quantitative PCR. We found that the expression level of serum mir-21 in the MM group was significantly higher than the MGUS group and the NC group (P < 0.01). According to the ISS installment, the level of mir-21, lgG, κ, and ALB in the MM group in stage I differed from that in stages II and III. The level of IgA, β2-MG in stage III was higher as compared with stage I and II (P < 0.05 and P < 0.01).The levels of mir-21, κ, (κ+λ), IgG, (IgG + IgA + IgM), and β2-MG in MM patients were positively correlated with ALB (P < 0.01). Based on the results, miR-21 plays an important role as an oncogene. Mir-21 may be important in the occurrence, development, and disease prognosis of MM.
authors.affiliation: The Laboratory Medicine of First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Wang
authors.affiliation: The Laboratory Medicine of First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Zhou
authors.affiliation: The Laboratory Medicine of First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: B X
authors.initials: BX
authors.lastname: Liu
authors.affiliation: The Laboratory Medicine of First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: D L
authors.initials: DL
authors.lastname: Man
authors.affiliation: The Laboratory Medicine of First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory of Medical Cell Biology.
authors.forename: F R
authors.initials: FR
authors.lastname: Liu
authors.affiliation: The Laboratory Medicine of First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: H
authors.initials: H
authors.lastname: Shang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D008998:Monoclonal Gammopathy of Undetermined Significance / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D009101:Multiple Myeloma / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D007136:Immunoglobulins; C524940:MIRN21 microRNA, human; D035683:MicroRNAs