title: Myocardial perfusion of infarcted and normal myocardium in propofol-anesthetized minipigs using (82)Rubidium PET.
abstract: Cardiac Rubidium-82 ((82)Rb) positron-emission-tomography (PET) is a good method for quantification of myocardial blood flow in man. Quantification of myocardial blood flow in animals to evaluate new treatment strategies or to understand underlying disease is also of great interest but raises some challenges. Animals, which have been anesthetized during PET acquisition, might react differently to used stress medications, and therefore difficulties might exist while evaluating the resulting PET images using standard software packages from commercial vendors optimized for human hearts. Furthermore propofol, used for anesthesia, can influence myocardial perfusion and coronary flow reserve due to its vasorelaxant effect, and interactions might exist between propofol and used stress agents, potentially affecting the result of the examination. We present cardiac (82)Rb-PET studies performed in propofol-anesthetized minipigs with normal and infarcted myocardium stressed with both adenosine and dipyridamole. Despite the mentioned challenges, we were able to trace the small minipig heart with software designed for human cardiac PET and to achieve blood flow measurements comparable with results in humans with both adenosine and dipyridamole. We found dipyridamole to be a superior stress agent for this experimental setup. Finally, we were able to clearly identify the myocardial perfusion defect after an induced myocardial infarction.
authors.affiliation: Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark. drrasmussen@hotmail.com.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rasmussen
authors.affiliation: Department of Cardiology, Cardiology Stem Cell Centre, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Bjarke
authors.initials: B
authors.lastname: Follin
authors.affiliation: Department of Cardiology, Cardiology Stem Cell Centre, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jens
authors.initials: J
authors.lastname: Kastrup
authors.affiliation: Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark.
authors.forename: Thomas Emil
authors.initials: TE
authors.lastname: Christensen
authors.affiliation: Department of Experimental Medicine, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Karsten Pharao
authors.initials: KP
authors.lastname: Hammelev
authors.affiliation: Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kjær
authors.affiliation: Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark.
authors.forename: Philip
authors.initials: P
authors.lastname: Hasbak
mesh_terms: D018686:Anesthetics, Intravenous / Q000008:administration & dosage; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D003326:Coronary Circulation / Q000187:drug effects*; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D055414:Myocardial Perfusion Imaging / Q000379:methods*; D049268:Positron-Emission Tomography / Q000379:methods*; D015742:Propofol / Q000008:administration & dosage*; D019275:Radiopharmaceuticals; D012016:Reference Values; D012414:Rubidium Radioisotopes; D013552:Swine; D013556:Swine, Miniature
chemical_list: D018686:Anesthetics, Intravenous; D019275:Radiopharmaceuticals; D012414:Rubidium Radioisotopes; C000615479:Rubidium-82; D015742:Propofol
keywords: A2A adenosine receptor agonists; PET; coronary artery disease; infarction; myocardial; myocardial perfusion imaging; pharmacologic stress