title: Growth and the Environment of Schizosaccharomyces pombe.
abstract: Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures. We discuss the concept of "culture memory," in which the growth state and stress experienced by a strain during storage, propagation, and starter culture preparation can alter experimental outcomes at later stages. We also describe the triggers that are widely used to manipulate signaling through the environment sensing pathways.
authors.affiliation: Flinders University, Flinders Centre for Innovation in Cancer, School of Medicine, FMST, Bedford Park, Adelaide, South Australia 5042, Australia;
authors.forename: Janni
authors.initials: J
authors.lastname: Petersen
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037.
authors.forename: Paul
authors.initials: P
authors.lastname: Russell
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D008828:Microbiological Techniques / Q000379:methods*; D012568:Schizosaccharomyces / Q000254:growth & development*
chemical_list: D003470:Culture Media