title: Evaluation of indigenous fresh water microalga Scenedesmus obtusus for feed and fuel applications: Effect of carbon dioxide, light and nutrient sources on growth and biochemical characteristics.
abstract: Scenedesmus obtusus, a freshwater microalga, was evaluated for its growth and biochemical characteristics under various culture conditions. S. obtusus was tolerant at all tested CO2 concentrations up to 20%. Among the different nitrogen sources, urea showed enhanced biomass productivities up to 2-fold compared to control, where the nitrogen source was sodium nitrate. Light intensity and photoperiod had a significant effect on growth rate and biomass productivity. The growth rate was observed maximum under continuous light exposure at the light intensities, 30μmolm(-2)sec(-1) and 60μmolm(-2)sec(-1) The species was able to tolerate the salinity levels up to 25mM NaCl, where, the increase in the concentration of NaCl suppressed the growth. Ammonium acetate and glycine showed better growth rate and biomass productivity indicating mixotrophic ability of S. obtusus. Supplementation of acetate and bicarbonate significantly enhanced the biomass productivity. Biodiesel properties of S. obtusus cultivated at various culture conditions were estimated.
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, India; Environmental Biotechnology Division, CSIR-National Environmental Engineering Research Institute, Nagpur, Maharashtra, India; Center for Environmental Science and Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India.
authors.forename: T
authors.initials: T
authors.lastname: Sarat Chandra
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Deepak
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, India.
authors.forename: M
authors.initials: M
authors.lastname: Maneesh Kumar
authors.affiliation: Center for Environmental Science and Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India.
authors.forename: S
authors.initials: S
authors.lastname: Mukherji
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, India.
authors.forename: V S
authors.initials: VS
authors.lastname: Chauhan
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, India.
authors.forename: R
authors.initials: R
authors.lastname: Sarada
authors.affiliation: Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysuru, Karnataka, India. Electronic address: sn.mudliar@gmail.com.
authors.forename: S N
authors.initials: SN
authors.lastname: Mudliar
mesh_terms: D000085:Acetates / Q000494:pharmacology; D001639:Bicarbonates / Q000494:pharmacology; D056804:Biofuels; D018533:Biomass; D002245:Carbon Dioxide / Q000737:chemistry / Q000494:pharmacology; D003470:Culture Media; D005998:Glycine / Q000494:pharmacology; D008027:Light; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D009584:Nitrogen / Q000737:chemistry / Q000494:pharmacology; D017440:Photoperiod; D054712:Salinity; D044444:Scenedesmus / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects
chemical_list: D000085:Acetates; D001639:Bicarbonates; D056804:Biofuels; D003470:Culture Media; D002245:Carbon Dioxide; D009584:Nitrogen; C018824:ammonium acetate; D005998:Glycine
keywords: Biodiesel; CO(2) concentration; Nitrogen source; Photoperiod; Scenedesmus obtusus