title: Supplementation with a new trypsin inhibitor from peanut is associated with reduced fasting glucose, weight control, and increased plasma CCK secretion in an animal model.
abstract: Ingestion of peanuts may have a beneficial effect on weight control, possibly due to the satietogenic action of trypsin inhibitors. The aim of this study was to isolate a new trypsin inhibitor in a typical Brazilian peanut sweet (paçoca) and evaluate its effect in biochemical parameters, weight gain and food intake in male Wistar rats. The trypsin inhibitor in peanut paçoca (AHTI) was isolated. Experimental diets were prepared with AIN-93G supplemented with AHTI. Animals had their weight and food intake monitored. Animals were anesthetized, euthanized, and their bloods collected by cardiac puncture for dosage of cholecystokinin (CCK) and other biochemical parameters. Supplementation with AHTI significantly decreased fasting glucose, body weight gain, and food intake. These effects may be attributed to increased satiety, once supplemented animals showed no evidence of impaired nutritional status and also because AHTI increased CCK production. Thus, our results indicate that AHTI, besides reducing fasting glucose, can reduce weight gain via food intake reduction.
authors.affiliation: a Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande Do Norte , Natal , RN , Brazil .
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Serquiz
authors.affiliation: a Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande Do Norte , Natal , RN , Brazil .
authors.forename: Richele J A
authors.initials: RJ
authors.lastname: Machado
authors.affiliation: a Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande Do Norte , Natal , RN , Brazil .
authors.forename: Raphael P
authors.initials: RP
authors.lastname: Serquiz
authors.affiliation: a Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande Do Norte , Natal , RN , Brazil .
authors.forename: Vanessa C O
authors.initials: VC
authors.lastname: Lima
authors.affiliation: d Postgraduate Nutrition Program, Center for Health Sciences, Federal University of Rio Grande Do Norte , Natal , RN , Brazil.
authors.forename: Fabiana Maria C
authors.initials: FM
authors.lastname: de Carvalho
authors.affiliation: b Course of Biological Sciences, Faculty of Science and Culture Extension of Rio Grande Do Norte , Natal , RN , Brazil .
authors.forename: Marcella A A
authors.initials: MA
authors.lastname: Carneiro
authors.affiliation: c Department of Nutrition , Center for Health Sciences, Federal University of Rio Grande Do Norte , Natal , RN , Brazil , and.
authors.forename: Bruna L L
authors.initials: BL
authors.lastname: Maciel
authors.affiliation: a Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande Do Norte , Natal , RN , Brazil .
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Uchôa
authors.affiliation: a Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande Do Norte , Natal , RN , Brazil .
authors.forename: Elizeu A
authors.initials: EA
authors.lastname: Santos
authors.affiliation: c Department of Nutrition , Center for Health Sciences, Federal University of Rio Grande Do Norte , Natal , RN , Brazil , and.|d Postgraduate Nutrition Program, Center for Health Sciences, Federal University of Rio Grande Do Norte , Natal , RN , Brazil.
authors.forename: Ana H A
authors.initials: AH
authors.lastname: Morais
mesh_terms: D000818:Animals; D010367:Arachis / Q000737:chemistry*; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight; D002766:Cholecystokinin / Q000097:blood* / Q000378:metabolism; D019587:Dietary Supplements; D005215:Fasting; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D014361:Trypsin Inhibitors / Q000008:administration & dosage*
chemical_list: D001786:Blood Glucose; D014361:Trypsin Inhibitors; D002766:Cholecystokinin
keywords: Arachis hypogaea L.; bioactive proteins; obesity; satiety