title: Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress.
abstract: H2S plays important physiological and pathological roles in the cardiovascular system and nervous system. However, recent evidence imply that hydrogen polysulfides (H2Sn) are the actual signaling molecules in cells. Although H2Sn have been demonstrated to be responsible for mediating tumor suppressors, ion channels, and transcription factors, more of their biological effects are still need to be elaborated. On one hand, H2Sn have been suggested to be generated from endogenous H2S upon reaction with reactive oxygen species (ROS). On the other hand, H2Sn derivatives are proposed to be a kind of direct antioxidant against intracellular oxidative stress. This conflicting results should be attributed to the regulation of redox homeostasis between ROS and H2Sn. Superoxide anion (O2(•-)) is undoubtedly the primary ROS existing in mitochondria. We reason that the balance of O2(•-) and H2Sn are pivotal in physiological and pathological processes. Herein, we report two near-infrared fluorescent probes Hcy-Mito and Hcy-Biot for the detection of O2(•-) and H2Sn in cells and in vivo. Hcy-Mito is conceived to be applied in mitochondria, and Hcy-Biot is designed to target tumor tissue. Both of the probes were successfully applied for visualizing exogenous and endogenous O2(•-) and H2Sn in living cells and in tumor mice models. The results demonstrate that H2Sn can be promptly produced by mitochondrial oxidative stress. Flow cytometry assays for apoptosis suggest that H2Sn play critical roles in antioxidant systems.
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai 264003, China.|Department of Chemistry, Qinghai Normal University , Xining 810008, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai 264003, China.
authors.forename: Fabiao
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Chemistry, Qinghai Normal University , Xining 810008, China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences , Yantai 264003, China.|College of Life Science, Yantai University , Yantai 264005, China.
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D000838:Anions / Q000032:analysis / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006859:Hydrogen / Q000032:analysis* / Q000378:metabolism; D007259:Infrared Rays; D017114:Liver Failure, Acute / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D018384:Oxidative Stress; D012509:Sarcoma / Q000737:chemistry / Q000378:metabolism; D013440:Sulfides / Q000032:analysis* / Q000378:metabolism; D013481:Superoxides / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000838:Anions; D005456:Fluorescent Dyes; D013440:Sulfides; D013481:Superoxides; D006859:Hydrogen; C032915:polysulfide