title: Selective regulation of pyrethrin biosynthesis by the specific blend of wound induced volatiles in Tanacetum cinerariifolium.
abstract: Natural pyrethrins are used to control household and agricultural pests, and it is of value to understand biosynthesis in Tanacetum cinerariifolium for enhanced production. We previously found that a blend of four green leaf volatiles (GLVs) and (E)-β-farnesene emitted by T. cinerariifolium seedlings enhanced gene expressions of certain biosynthetic enzymes in unwounded seedlings; however, the extent to which such a regulation facilitates pyrethrin biosynthesis remains unknown. Here we have investigated the effects of the blend of the volatile organic compounds (VOCs) on gene expressions of seven biosynthetic enzymes. VOC treatment resulted in enhanced chrysanthemyl diphosphate synthase (CDS), chrysanthemic acid synthase (CAS), Tanacetum cinerariifolium GDSL lipase (TcGLIP) and acyl-Coenzyme A oxidase 1 (ACX1) gene expressions that reached a peak at a 12 h VOC treatment, whereas the treatment minimally influenced the expressions of other biosynthetic genes. In undifferentiated Tanacetum tissues, such VOC-induced amplification of CDS, CAS, TcGLIP and ACX1 gene expressions were markedly reduced, suggesting that a high-resolution, VOC-mediated communication is an event selective to differentiated plants.
authors.affiliation: a Graduate School of Agriculture, Faculty of Agriculture, Kinki University , Nakamachi, Nara , Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Sakamori
authors.affiliation: b Nara Institute of Science and Technology, Takayama , Ikoma, Nara , Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Ono
authors.affiliation: a Graduate School of Agriculture, Faculty of Agriculture, Kinki University , Nakamachi, Nara , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Ihara
authors.affiliation: c Kazusa DNA Research Institute , Kazusa-kamatari, Kisarazu, Chiba , Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: d Research Faculty of Agriculture, Division of Applied Bioscience, Hokkaido University , Kita-ku, Sapporo, Hokkaido , Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.affiliation: e Division of Pharmacognosy, College of Pharmaceutical Science, Ritsumeikan University , Noji-higashi, Kusatsu, Shiga , Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: f Graduate School of Life and Environmental Sciences, Osaka Prefecture University , Nakaku, Sakai, Osaka , Japan.
authors.forename: Daisaku
authors.initials: D
authors.lastname: Ohta
authors.affiliation: b Nara Institute of Science and Technology, Takayama , Ikoma, Nara , Japan.
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Kanaya
authors.affiliation: a Graduate School of Agriculture, Faculty of Agriculture, Kinki University , Nakamachi, Nara , Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuda
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D011723:Chrysanthemum cinerariifolium / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D011722:Pyrethrins / Q000737:chemistry / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000378:metabolism*
chemical_list: D011722:Pyrethrins; D055549:Volatile Organic Compounds
keywords: (E)-β-farnesene; Biosynthesis; gene expression analysis; green Leaf volatiles; pyrethrins; tanacetum cinerariifolium; volatile organic compounds