title: Histologic evaluation of Ki-67 and cleaved caspase-3 expression in feline mammary carcinoma.
abstract: Objectives The purpose of this study was to evaluate histopathologic aspects of, and the expression of Ki-67 and cleaved caspase-3 in, feline mammary carcinoma (FMC). Methods Feline mammary tumors were surgically obtained by mastectomy from 30 female cats and were fixed with formalin and embedded in paraffin wax. Four-micron sections were stained with hematoxylin and eosin for histopathologic diagnosis. Ki-67 and cleaved caspase-3 were analyzed by immunohistochemistry. Results Samples were histologically confirmed as FMC. Positive immunostaining was observed in all cancer samples for both nuclear Ki-67 and cleaved caspase-3, with a mean positive staining percentage of 27.5% and 21.2%, respectively. No statistically significant correlations between Ki-67 and cleaved caspase-3 were observed within FMC. Conclusions and relevance A high proliferation index was found in feline mammary tumors. This is the first study evaluating cleaved caspase-3 expression in FMC.
authors.affiliation: 1 Postgraduate Program in Pathology, Fluminense Federal University, Niterói, RJ, Brazil.
authors.forename: Millena N
authors.initials: MN
authors.lastname: Silva
authors.affiliation: 2 Department of Pathology and Veterinary Clinics, Faculty of Veterinary Medicine, Fluminense Federal University Niteroi, RJ, Brazil.
authors.forename: Juliana S
authors.initials: JS
authors.lastname: Leite
authors.affiliation: 2 Department of Pathology and Veterinary Clinics, Faculty of Veterinary Medicine, Fluminense Federal University Niteroi, RJ, Brazil.
authors.forename: Marcela Fv
authors.initials: MF
authors.lastname: Mello
authors.affiliation: 3 Postgraduate Program in Clinical and Animal Reproduction, Faculty of Veterinary Medicine, Fluminense Federal University, Niteroi, RJ, Brazil.
authors.forename: Kassia Vgc
authors.initials: KV
authors.lastname: Silva
authors.affiliation: 3 Postgraduate Program in Clinical and Animal Reproduction, Faculty of Veterinary Medicine, Fluminense Federal University, Niteroi, RJ, Brazil.
authors.forename: Katia B
authors.initials: KB
authors.lastname: Corgozinho
authors.affiliation: 4 Department of Clinical and Surgery, Veterinary Institute, Federal Rural University of Rio de Janeiro, RJ, Brazil.
authors.forename: Heloisa Jm
authors.initials: HJ
authors.lastname: de Souza
authors.affiliation: 3 Postgraduate Program in Clinical and Animal Reproduction, Faculty of Veterinary Medicine, Fluminense Federal University, Niteroi, RJ, Brazil.
authors.forename: Simone Cs
authors.initials: SC
authors.lastname: Cunha
authors.affiliation: 2 Department of Pathology and Veterinary Clinics, Faculty of Veterinary Medicine, Fluminense Federal University Niteroi, RJ, Brazil.|3 Postgraduate Program in Clinical and Animal Reproduction, Faculty of Veterinary Medicine, Fluminense Federal University, Niteroi, RJ, Brazil.
authors.forename: Ana Mr
authors.initials: AM
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000032:analysis* / Q000276:immunology; D002371:Cat Diseases / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D002415:Cats; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry / Q000662:veterinary; D019394:Ki-67 Antigen / Q000032:analysis* / Q000276:immunology; D015674:Mammary Neoplasms, Animal / Q000235:genetics / Q000276:immunology / Q000473:pathology*
chemical_list: D019394:Ki-67 Antigen; D053148:Caspase 3