title: Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production.
abstract: Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, SE 230 53, Alnarp, Sweden.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zhu
authors.affiliation: Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands.
authors.forename: Frans
authors.initials: F
authors.lastname: Krens
authors.affiliation: National Research Council of Canada, 110 Gymnasium Place, Saskatoon, SK S7N 0W9, Canada.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Smith
authors.affiliation: Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 101, SE 230 53, Alnarp, Sweden.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands.
authors.forename: Weicong
authors.initials: W
authors.lastname: Qi
authors.affiliation: Wageningen UR Plant Breeding, P.O. Box 386, 6700 AJ Wageningen, The Netherlands.
authors.forename: Eibertus N
authors.initials: EN
authors.lastname: van Loo
authors.affiliation: Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, Justus-von-Liebig-Weg 11, 37077, Göttingen, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Iven
authors.affiliation: Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, Justus-von-Liebig-Weg 11, 37077, Göttingen, Germany.|Department of Plant Biochemistry, Goettingen Center for Molecular Biosciences (GZMB), Georg-August-University, Justus-von-Liebig-Weg 11, 37077, Göttingen, Germany.|Department of Plant Biochemistry, International Center for Advanced Studies of Energy Conversion (ICASEC), Georg-August-University, Justus-von-Liebig-Weg 11, 37077, Göttingen, Germany.
authors.forename: Ivo
authors.initials: I
authors.lastname: Feussner
authors.affiliation: Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: Tara J
authors.initials: TJ
authors.lastname: Nazarenus
authors.affiliation: Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.|National Key Lab of Crop Genetic Improvement and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dongxin
authors.initials: D
authors.lastname: Huai
authors.affiliation: National Research Council of Canada, 110 Gymnasium Place, Saskatoon, SK S7N 0W9, Canada.
authors.forename: David C
authors.initials: DC
authors.lastname: Taylor
authors.affiliation: CSIRO Food &Nutrition, North Ryde, Sydney, NSW, Australia.|CSIRO Agriculture, Canberra, ACT, Australia.
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Zhou
authors.affiliation: CSIRO Agriculture, Canberra, ACT, Australia.
authors.forename: Allan G
authors.initials: AG
authors.lastname: Green
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, Commodity Utilization Research Unit, New Orleans, LA 70124, USA.
authors.forename: Jay
authors.initials: J
authors.lastname: Shockey
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, Commodity Utilization Research Unit, New Orleans, LA 70124, USA.
authors.forename: K Thomas
authors.initials: KT
authors.lastname: Klasson
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Mullen
authors.affiliation: College of Life Science, Hubei University, Wuhan 430062, P. R. China.
authors.forename: Bangquan
authors.initials: B
authors.lastname: Huang
authors.affiliation: U.S. Department of Agriculture-Agricultural Research Service, U.S. Arid-Land Agricultural Research Center, Maricopa, AZ 85138, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Dyer
authors.affiliation: Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.
authors.forename: Edgar B
authors.initials: EB
authors.lastname: Cahoon
mesh_terms: D019149:Bioreactors; D019607:Brassicaceae / Q000235:genetics / Q000378:metabolism*; D018556:Crops, Agricultural / Q000235:genetics / Q000378:metabolism*; D060847:Metabolic Engineering; D010938:Plant Oils / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014885:Waxes / Q000378:metabolism*
chemical_list: D010938:Plant Oils; D014885:Waxes