title: Three-dimensional WS2 nanosheet networks for H2O2 produced for cell signaling.
abstract: Hydrogen peroxide (H2O2) is an important molecular messenger for cellular signal transduction. The capability of direct probing of H2O2 in complex biological systems can offer potential for elucidating its manifold roles in living systems. Here we report the fabrication of three-dimensional (3D) WS2 nanosheet networks with flower-like morphologies on a variety of conducting substrates. The semiconducting WS2 nanosheets with largely exposed edge sites on flexible carbon fibers enable abundant catalytically active sites, excellent charge transfer, and high permeability to chemicals and biomaterials. Thus, the 3D WS2-based nano-bio-interface exhibits a wide detection range, high sensitivity and rapid response time for H2O2, and is capable of visualizing endogenous H2O2 produced in living RAW 264.7 macrophage cells and neurons. First-principles calculations further demonstrate that the enhanced sensitivity of probing H2O2 is attributed to the efficient and spontaneous H2O2 adsorption on WS2 nanosheet edge sites. The combined features of 3D WS2 nanosheet networks suggest attractive new opportunities for exploring the physiological roles of reactive oxygen species like H2O2 in living systems.
authors.affiliation: Laboratory of Advanced Materials, Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China. gfzheng@fudan.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.affiliation: Laboratory of Advanced Materials, Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China. gfzheng@fudan.edu.cn.
authors.forename: Yingzhou
authors.initials: Y
authors.lastname: Quan
authors.affiliation: Key Laboratory of Computational Physical Sciences, Ministry of Education, State Key Laboratory of Surface Physics, and Department of Physics, Fudan University, Shanghai 200433, China.
authors.forename: Yueyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Abdullah M
authors.initials: AM
authors.lastname: Al-Enizi
authors.affiliation: Laboratory of Advanced Materials, Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China. gfzheng@fudan.edu.cn.
authors.forename: Biao
authors.initials: B
authors.lastname: Kong
authors.affiliation: Laboratory of Advanced Materials, Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China. gfzheng@fudan.edu.cn.
authors.forename: Tiance
authors.initials: T
authors.lastname: An
authors.affiliation: Department of Cardiovascular Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Wenshuo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Xia
authors.affiliation: Key Laboratory of Computational Physical Sciences, Ministry of Education, State Key Laboratory of Surface Physics, and Department of Physics, Fudan University, Shanghai 200433, China.
authors.forename: Xingao
authors.initials: X
authors.lastname: Gong
authors.affiliation: Laboratory of Advanced Materials, Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China. gfzheng@fudan.edu.cn.
authors.forename: Gengfeng
authors.initials: G
authors.lastname: Zheng
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques; D002384:Catalysis; D020134:Catalytic Domain; D006861:Hydrogen Peroxide / Q000737:chemistry*; D021621:Imaging, Three-Dimensional / Q000379:methods; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D009474:Neurons / Q000378:metabolism; D010539:Permeability; D000067996:RAW 264.7 Cells; D015203:Reproducibility of Results; D015398:Signal Transduction; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D006861:Hydrogen Peroxide