title: Development of a disaggregation-induced emission probe for the detection of RecA inteins from Mycobacterium tuberculosis.
abstract: A fluorescent sensor - InR - with disaggregation-induced emission (DIE) characteristics has been developed for the label free detection of RecA inteins from Mycobacterium tuberculosis. Mechanistic studies demonstrate that InR binds at a hydrophobic pocket of RecA inteins, which is constituted by Gly435, Glu434, Arg405 and Arg 7, resulting in the disaggregation and recovery of its fluorescence.
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China. zly0605@ustc.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Hefei National Laboratory for Physical Science at the Microscale, Core Facility Center for Life Sciences, School of Life Sciences, University of Science and Technology of China, Hefei 230026, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Hefei National Laboratory for Physical Science at the Microscale, Core Facility Center for Life Sciences, School of Life Sciences, University of Science and Technology of China, Hefei 230026, P. R. China.
authors.forename: Zhaofeng
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 2, 117543, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
authors.affiliation: Institute of Technical Biology and Agriculture Engineering, Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China. zly0605@ustc.edu.cn.
authors.forename: Liyun
authors.initials: L
authors.lastname: Zhang
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D047668:Inteins; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D011938:Rec A Recombinases / Q000032:analysis* / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D011938:Rec A Recombinases