title: Detection of a Novel gyrB Mutation Associated With Fluoroquinolone-Nonsusceptible Salmonella enterica serovar Typhimurium Isolated From a Bloodstream Infection in Ghana.
abstract: A multidrug-resistant Salmonella enterica serovar Typhimurium with reduced susceptibility to ciprofloxacin was isolated from the blood of a hospitalized child in Ghana. DNA sequencing identified a novel gyrB mutation at codon 466 (Glu466Asp). An increase in fluoroquinolone susceptibility after the introduction of a wild-type gyrB(+) allele demonstrated that the gyrB466 mutation had a direct effect on fluoroquinolone susceptibility.
authors.affiliation: Bernhard-Nocht Institute for Tropical Medicine German Center for Infection Research, partner site Hamburg-Borstel-Lübeck.
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Al-Emran
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Hamburg, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Heisig
authors.affiliation: Bernhard-Nocht Institute for Tropical Medicine German Center for Infection Research, partner site Hamburg-Borstel-Lübeck.
authors.forename: Denise
authors.initials: D
authors.lastname: Dekker
authors.affiliation: Kumasi Centre for Collaborative Research in Tropical Medicine, Ghana.
authors.forename: Yaw
authors.initials: Y
authors.lastname: Adu-Sarkodie
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Ligia Maria
authors.initials: LM
authors.lastname: Cruz Espinoza
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Ursula
authors.initials: U
authors.lastname: Panzner
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Vera
authors.initials: V
authors.lastname: von Kalckreuth
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Florian
authors.initials: F
authors.lastname: Marks
authors.affiliation: International Vaccine Institute, Seoul, Republic of Korea.
authors.forename: Se Eun
authors.initials: SE
authors.lastname: Park
authors.affiliation: Kumasi Centre for Collaborative Research in Tropical Medicine, Ghana.
authors.forename: Nimako
authors.initials: N
authors.lastname: Sarpong
authors.affiliation: Bernhard-Nocht Institute for Tropical Medicine German Center for Infection Research, partner site Hamburg-Borstel-Lübeck.
authors.forename: Jürgen
authors.initials: J
authors.lastname: May
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Hamburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Heisig
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics*; D027081:DNA Gyrase / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D006801:Humans; D007223:Infant; D009154:Mutation / Q000235:genetics; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D024841:Fluoroquinolones; D027081:DNA Gyrase
keywords: Typhimurium; fluoroquinolone; gyrB; resistance