title: Proteomic changes in maize as a response to heavy metal (lead) stress revealed by iTRAQ quantitative proteomics.
abstract: Lead (Pb), a heavy metal, has become a crucial pollutant in soil and water, causing not only permanent and irreversible health problems, but also substantial reduction in crop yields. In this study, we conducted proteome analysis of the roots of the non-hyperaccumulator inbred maize line 9782 at four developmental stages (0, 12, 24, and 48 h) under Pb pollution using isobaric tags for relative and absolute quantification technology. A total of 252, 72 and 116 proteins were differentially expressed between M12 (after 12-h Pb treatment) and CK (water-mocked treatment), M24 (after 24-h Pb treatment) and CK, and M48 (after 48-h Pb treatment) and CK, respectively. In addition, 14 differentially expressed proteins were common within each comparison group. Moreover, Cluster of Orthologous Groups enrichment analysis revealed predominance of the proteins involved in posttranslational modification, protein turnover, and chaperones. Additionally, the changes in protein profiles showed a lower concordance with corresponding alterations in transcript levels, indicating important roles for transcriptional and posttranscriptional regulation in the response of maize roots to Pb pollution. Furthermore, enriched functional categories between the successive comparisons showed that the proteins in functional categories of stress, redox, signaling, and transport were highly up-regulated, while those in the functional categories of nucleotide metabolism, amino acid metabolism, RNA, and protein metabolism were down-regulated. This information will help in furthering our understanding of the detailed mechanisms of plant responses to heavy metal stress by combining protein and mRNA profiles.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture.|Guangdong Provincial Key Laboratory of Crops Genetics and Improvement, Guangdong Academy of Agricultural Sciences, Crop Research Institute, Guangzhou, China.
authors.forename: G K
authors.initials: GK
authors.lastname: Li
authors.affiliation: Key Laboratory of Tumor Immunopathology, Institute of Pathology and Southwest Cancer Center, Southwest Hospital.
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture.|Sichuan Tourism University, Chengdu, Sichuan, China.
authors.forename: H
authors.initials: H
authors.lastname: Peng
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture.
authors.forename: Y O
authors.initials: YO
authors.lastname: Shen
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture.
authors.forename: H P
authors.initials: HP
authors.lastname: Ding
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture.
authors.forename: G T
authors.initials: GT
authors.lastname: Pan
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture.
authors.forename: H J
authors.initials: HJ
authors.lastname: Lin
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D007854:Lead / Q000633:toxicity*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D012989:Soil Pollutants / Q000633:toxicity*; D013312:Stress, Physiological; D059467:Transcriptome; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002352:Carrier Proteins; D018832:Molecular Chaperones; D010940:Plant Proteins; D020543:Proteome; D018749:RNA, Plant; D012989:Soil Pollutants; D007854:Lead