title: Reaction of PerR with Molecular Oxygen May Assist H2O2 Sensing in Anaerobes.
abstract: PerR is the peroxide resistance regulator found in several pathogenic bacteria and governs their resistance to peroxide stress by inducing enzymes that destroy peroxides. However, it has recently been implicated as a key component of the aerotolerance in several facultative or strict anaerobes, including the highly pathogenic Staphylococcus aureus. By combining (18)O labeling studies to ESI- and MALDI-TOF MS detection and EMSA experiments, we demonstrate that the active form of PerR reacts with dioxygen, which leads ultimately to disruption of the PerR/DNA complex and is thus physiologically meaningful. Moreover, we show that the presence of O2 assists PerR sensing of H2O2, another feature likely to be important for anaerobic organisms. These results allow one to envisage different scenarios for the response of anaerobes to air exposure.
authors.affiliation: Université Grenoble Alpes, LCBM , F-38054 Grenoble, France.|CEA, DSV, BIG, LCBM, PMB , F-38054 Grenoble, France.|CNRS UMR 5249, LCBM , F-38054 Grenoble, France.
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Sethu
authors.affiliation: Université Grenoble Alpes, LCBM , F-38054 Grenoble, France.|CEA, DSV, BIG, LCBM, PMB , F-38054 Grenoble, France.|CNRS UMR 5249, LCBM , F-38054 Grenoble, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Gouré
authors.affiliation: Université Grenoble Alpes, IBS , F-38044 Grenoble, France.|CNRS, IBS , F-38044 Grenoble, France.|CEA, IBS , F-38044 Grenoble, France.
authors.forename: Luca
authors.initials: L
authors.lastname: Signor
authors.affiliation: Université Grenoble Alpes, LCBM , F-38054 Grenoble, France.|CEA, DSV, BIG, LCBM, PMB , F-38054 Grenoble, France.|CNRS UMR 5249, LCBM , F-38054 Grenoble, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Caux-Thang
authors.affiliation: Université Grenoble Alpes, LCBM , F-38054 Grenoble, France.|CEA, DSV, BIG, LCBM, PMB , F-38054 Grenoble, France.|CNRS UMR 5249, LCBM , F-38054 Grenoble, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Clémancey
authors.affiliation: Université Grenoble Alpes, LCBM , F-38054 Grenoble, France.|CEA, DSV, BIG, LCBM, PMB , F-38054 Grenoble, France.|CNRS UMR 5249, LCBM , F-38054 Grenoble, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Duarte
authors.affiliation: Université Grenoble Alpes, LCBM , F-38054 Grenoble, France.|CEA, DSV, BIG, LCBM, PMB , F-38054 Grenoble, France.|CNRS UMR 5249, LCBM , F-38054 Grenoble, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Latour
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; C122138:peroxide repressor proteins; D006861:Hydrogen Peroxide; D010100:Oxygen