title: Data on final calcium concentration in native gel reagents determined accurately through inductively coupled plasma measurements.
abstract: In this article we present data on the concentration of calcium as determined by Inductively Coupled Plasma (ICP) measurements. Calcium was estimated in the reagents used for native gel electrophoresis of Neuronal Calcium Sensor (NCS) proteins. NCS proteins exhibit calcium-dependent mobility shift in native gels. The sensitivity of this shift to calcium necessitated a precise determination of calcium concentrations in all reagents used. We determined the calcium concentrations in different components used along with the samples in the native gel experiments. These were: 20 mM Tris pH 7.5, loading dye and running buffer, with distilled water as reference. Calcium determinations were through ICP measurements. It was found that the running buffer contained calcium (244 nM) over the blank.
authors.affiliation: Graduate School of Biomedical Sciences, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Viviano
authors.affiliation: Graduate School of Biomedical Sciences, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Graduate School of Biomedical Sciences, USA.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Krishnan
authors.affiliation: Department of Chemistry, Rowan University, Glassboro, NJ 08028, USA.
authors.forename: Kandalam
authors.initials: K
authors.lastname: Ramanujachary
authors.affiliation: Graduate School of Biomedical Sciences, USA; School of Osteopathic Medicine, Rowan University, Stratford, NJ 08084, USA.
authors.forename: Venkat
authors.initials: V
authors.lastname: Venkataraman
keywords: Calcium; Electrophoresis; Magnesium; Mobility shift; Neuronal calcium sensor proteins