title: Bone marrow stromal cells inhibits HMGB1-mediated inflammation after stroke in type 2 diabetic rats.
abstract: High-mobility group box 1 (HMGB1), a ligand of receptor for advanced glycation endproducts (RAGE), functions as a proinflammatory factor. It is mainly involved in inflammatory activation and contributes to the initiation and progression of stroke. By using a model of transient middle cerebral artery occlusion (MCAo) in type 2 diabetic rats, we investigated the changes of pro-inflammation mediators, blood-brain barrier (BBB) leakage and functional outcome after stroke. Type 2 diabetic rats did not show an increased lesion volume, but exhibited significantly increased expression of HMGB1 and RAGE, BBB leakage, as well as decreased functional outcome after stroke compared with control rats. Injection of bone marrow stromal cells (BMSCs) into type 2 diabetic rats significantly reduced the expression of HMGB1 and RAGE, attenuated BBB leakage, and improved functional outcome after stroke. BMSCs-treated type 2 diabetic rats inhibited inflammation and improved functional outcome after stroke. Furthermore, in vitro data support the hypothesis that BMSCs-induced reduction of HMGB1 and RAGE in T2DM-MCAo rats contributed to attenuated inflammatory response in the ischemic brain, which may lead to the beneficial effects of BMSCs treatment. Further investigation of BMSCs treatment in type 2 diabetic stroke is warranted.
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China.
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China; Department of Geriatric Neurology, Nanjing Brain Hospital, Affiliated to Nanjing Medical University, Nanjing, Jiangsu Province, China.
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Neurology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Plastic Surgery, Xuzhou Central Hospital, Xuzhou, Jiangsu Province, China.
authors.forename: P
authors.initials: P
authors.lastname: Jin
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China.
authors.forename: F
authors.initials: F
authors.lastname: Hua
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China.
authors.forename: K
authors.initials: K
authors.lastname: Zan
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China. Electronic address: guiyuncui@foxmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Cui
authors.affiliation: Department of Neurology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu Province, China. Electronic address: xinchunye@gmail.com.
authors.forename: X
authors.initials: X
authors.lastname: Ye
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000276:immunology / Q000473:pathology; D002199:Capillary Permeability / Q000276:immunology; D015687:Cell Hypoxia / Q000276:immunology; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000276:immunology* / Q000473:pathology; D005947:Glucose / Q000172:deficiency; D024243:HMGB1 Protein / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery; D007249:Inflammation / Q000209:etiology / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology; D017628:Microglia / Q000378:metabolism; D017208:Rats, Wistar; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism; D020127:Recovery of Function / Q000276:immunology; D020521:Stroke / Q000150:complications / Q000276:immunology* / Q000473:pathology / Q000628:therapy*
chemical_list: C000593874:Ager protein, rat; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D000067759:Receptor for Advanced Glycation End Products; D005947:Glucose
keywords: bone marrow stromal cells; high-mobility group box 1; inflammation; ischemic stroke; receptor for advanced glycation endproducts; type 2 diabetes mellitus