title: Reversible Photoregulation of Gene Expression and Translation.
abstract: Several methods for controlling gene expression by light illumination have been reported. Most of these methods control transcription by regulating the interaction between DNA and transcription factors. The use of a photolabile protecting compound (cage compound) is another promising approach for controlling gene expression, although typically in an irreversible manner. We here describe a new approach for reversibly controlling translation using a photoresponsive 8-styryl cap (8ST-cap) that can be reversibly isomerized by illumination with light of a specific wavelength.
authors.affiliation: Creative Research Institution Sousei (CRIS), Hokkaido University, Kita 21 Nishi 10, Kita-ku, Sapporo, Hokkaido, 001-0021, Japan. shinzi@cris.hokudai.ac.jp.
authors.forename: Shinzi
authors.initials: S
authors.lastname: Ogasawara
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007536:Isomerism; D008027:Light; D062308:Optogenetics / Q000379:methods*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D014162:Transfection / Q000379:methods
chemical_list: D012333:RNA, Messenger
keywords: Cap structure; Photoisomerization; Translation; mRNA