title: The comparison study of bioactivity between composites containing synthetic non-substituted and carbonate-substituted hydroxyapatite.
abstract: Apatite forming ability of hydroxyapatite (HAP) and carbonate hydroxyapatite (CHAP) containing composites was compared. Two composite materials, intended for filling bone defects, were made of polysaccharide polymer and one of two types of hydroxyapatite. The bioactivity of the composites was evaluated in vitro by soaking in a simulated body fluid (SBF), and the formation of the apatite layer was determined by scanning electron microscopy with energy-dispersive spectrometer and Raman spectroscopy. The results showed that both the composites induced the formation of apatite layer on their surface after soaking in SBF. In addition, the sample weight changes and the ion concentration of the SBF were scrutinized. The results showed the weight increase for both materials after SBF treatment, higher weight gain and higher uptake of calcium ions by HAP containing scaffolds. SBF solution analysis indicated loss of calcium and phosphorus ions during experiment. All these results indicate apatite forming ability of both biomaterials and suggest comparable bioactive properties of composite containing pure hydroxyapatite and carbonate-substituted one.
authors.affiliation: Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland. Electronic address: leszek.borkowski@umlub.pl.
authors.forename: Leszek
authors.initials: L
authors.lastname: Borkowski
authors.affiliation: Department of Biopharmacy, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sroka-Bartnicka
authors.affiliation: Department of Synthesis and Chemical Technology of Pharmaceutical Substances, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Drączkowski
authors.affiliation: Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ptak
authors.affiliation: SEM Laboratory, Department of Zoology and Ecology, John Paul II Catholic University of Lublin, Al. Kraśnicka 102, 20-718 Lublin, Poland.
authors.forename: Emil
authors.initials: E
authors.lastname: Zięba
authors.affiliation: Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Mickiewicza 30, 30-059 Krakow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ślósarczyk
authors.affiliation: Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Ginalska
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D002254:Carbonates / Q000737:chemistry*; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D007477:Ions / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010758:Phosphorus / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013059:Spectrum Analysis, Raman
chemical_list: D001672:Biocompatible Materials; D002254:Carbonates; D007477:Ions; D010758:Phosphorus; D017886:Durapatite; D002118:Calcium
keywords: Apatite; Bioactivity; Bone; Composite; Raman spectroscopy