title: Carcinoma-Associated Fibroblasts Lead the Invasion of Salivary Gland Adenoid Cystic Carcinoma Cells by Creating an Invasive Track.
abstract: Carcinoma-associated fibroblasts (CAFs) are critical in determining tumor invasion and metastasis. However the role of CAFs in the invasion of salivary gland adenoid cystic carcinoma (ACC) is poorly understood. In this study, we isolated primary CAFs from two ACC patients. ACC-derived CAFs expressed typical CAF biomarkers and showed increased migration and invasion activity. Conditioned medium collected from CAFs significantly promoted ACC cell migration and invasion. Co-culture of CAFs with ACC cells in a microfluidic device further revealed that CAFs localized at the invasion front and ACC cells followed the track behind the CAFs. Interfering of both matrix metalloproteinase and CXCL12/CXCR4 pathway inhibited ACC invasion promoted by CAFs. Overall, our study demonstrates that ACC-derived CAFs exhibit the most important defining feature of CAFs by promoting cancer invasion. In addition to secretion of soluble factors, CAFs also lead ACC invasion by creating an invasive track in the ECM.
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Oral Surgery, the First Affiliated Hospital, Dalian Medical University, Dalian, China.
authors.forename: Zhuqiang
authors.initials: Z
authors.lastname: Jia
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Kong
authors.affiliation: Department of Oral Surgery, the Second Affiliated Hospital, Dalian Medical University, Dalian, China.
authors.forename: Fuyin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Shimeng
authors.initials: S
authors.lastname: Fang
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Wuwei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Faculty of Chemical, Environmental and Biological Science and Technology, Dalian Technology University, Dalian, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Faculty of Chemical, Environmental and Biological Science and Technology, Dalian Technology University, Dalian, China.|Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
authors.affiliation: College of Stomatology, Dalian Medical University, Dalian, China.
authors.forename: Tingjiao
authors.initials: T
authors.lastname: Liu
mesh_terms: D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000378:metabolism; D018920:Coculture Techniques; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D009363:Neoplasm Proteins; D019718:Receptors, CXCR4; D018093:Gelatinases