title: The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro.
abstract: OBJECTIVE
  This study focused on investigating the expression of several inflammatory cytokines and chemokines, including regulatory proteins in the astrocytes of mice stimulated with IL-17.
  
  
  METHODS
  The cultured astrocytes from the spinal cords of mice were stimulated with IL-17. The expression of interleukin-6 (IL-6), tumor necrosis factor (TNF), monocyte chemotactic protein-1/5 (MCP-1/5) and macrophage inflammatory protein-2 (MIP-2) stimulated with IL-17 (50 ng/ml) at different time points (3 h, 6 h, 12 h, 24 h and 48 h) were determined using real-time PCR and ELISA. The expressions of A20 (tumor necrosis factor α inducible protein 3, TNFAIP3) and NF-x03BA;B were examined using real-time PCR and western blotting.
  
  
  RESULTS
  Compared with the control group, the mRNA expression levels of IL-6, TNF, MCP-1/5 and MIP-2 increased significantly at 6 h after IL-17 stimulation, while the protein expression levels also increased significantly and peaked at 12 h. The mRNA expression level of NF-x03BA;B increased and peaked at 6 h before gradually declining, while the expression of A20 decreased. The protein expression level of NF-x03BA;B increased and peaked at 12 h, while the expression A20 had an opposite response.
  
  
  CONCLUSIONS
  The study showed that NF-x03BA;B may have an effect on the cytokines secreted by astrocytes after IL-17 stimulation. Moreover, both A20 and NF-x03BA;B could regulate the expression and secretion of inflammatory mediators.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Zong
authors.forename: Keke
authors.initials: K
authors.lastname: Li
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Zeng
authors.forename: Ye
authors.initials: Y
authors.lastname: Fang
authors.forename: Jingmin
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D020381:Interleukin-17 / Q000494:pharmacology*; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology*; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3 / Q000235:genetics* / Q000378:metabolism
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D016328:NF-kappa B; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3; C513261:Tnfaip3 protein, mouse