title: Molecular epidemiology of rotavirus in four provinces of Angola before vaccine introduction.
abstract: Angola is a sub-Saharan country in southern Africa highly affected by diarrhoeal disease with limited epidemiological data regarding etiologic agents. This study was performed during 2012-2013, prior to rotavirus vaccine introduction, with the objective to detect and characterize the rotavirus strains circulating in four provinces of the country: Huambo, Luanda, Zaire, and Cabinda. A high rotavirus detection rate (35%, 117/334) was observed. G1 was the most common G-genotype (83.6%), whereas P[8] (50.9%) followed by P[6] (38.8%) were the most common P-types. G1P[8] was identified as the predominant combination (50%), followed by the unusual G1P[6] (29.3%). Strains such G2P[4], G8P[6], G9P[6], and G12P[6] were also found in lower frequencies (5.2-1.7%). The P[6] strains did not cluster in the phylogenetic trees according to their geographic origin or even the corresponding G-genotype, suggesting a limited number of recent introductions and extensive reassortment events. Our results represent the first report on rotavirus genotype profiles in Angola, showing a wide circulation of the unusual genotype G1P[6], and underline the importance of RV surveillance after the vaccine introduction. J. Med. Virol. 88:1511-1520, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Global Health and Tropical Medicine (GHTM), Medical Microbiology Unit, Institute of Hygiene and Tropical Medicine, NOVA University of Lisbon, Lisbon, Portugal.
authors.forename: Aida
authors.initials: A
authors.lastname: Esteves
authors.affiliation: Medical Faculty, Division of Molecular Virology, Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Nordgren
authors.affiliation: Global Health and Tropical Medicine (GHTM), Medical Microbiology Unit, Institute of Hygiene and Tropical Medicine, NOVA University of Lisbon, Lisbon, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Pereira
authors.affiliation: Department of Disease Control, National Institute of Public Health, National Program for Malaria Control, Luanda, Angola.
authors.forename: Filomeno
authors.initials: F
authors.lastname: Fortes
authors.affiliation: Department of Disease Control, National Institute of Public Health, National Program for Malaria Control, Luanda, Angola.
authors.forename: Rafael
authors.initials: R
authors.lastname: Dimbu
authors.affiliation: Department of Disease Control, National Institute of Public Health, National Program for Malaria Control, Luanda, Angola.
authors.forename: Nilton
authors.initials: N
authors.lastname: Saraiva
authors.affiliation: Global Health and Tropical Medicine (GHTM), Medical Microbiology Unit, Institute of Hygiene and Tropical Medicine, NOVA University of Lisbon, Lisbon, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Mendes
authors.affiliation: Global Health and Tropical Medicine (GHTM), Medical Microbiology Unit, Institute of Hygiene and Tropical Medicine, NOVA University of Lisbon, Lisbon, Portugal.
authors.forename: Claudia
authors.initials: C
authors.lastname: Istrate
mesh_terms: D000352:Africa, Northern / Q000453:epidemiology; D000353:Africa, Southern / Q000453:epidemiology; D000810:Angola / Q000453:epidemiology; D000956:Antigens, Viral / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D015023:Democratic Republic of the Congo / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology; D005838:Genotype; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D022243:Rotavirus Vaccines
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; D022243:Rotavirus Vaccines
keywords: children; diarrhoeal disease; genotypes