title: High-resolution genetic linkage mapping, high-temperature tolerance and growth-related quantitative trait locus (QTL) identification in Marsupenaeus japonicus.
abstract: The Kuruma prawn, Marsupenaeus japonicus, is one of the most promising marine invertebrates in the industry in Asia, Europe and Australia. However, the increasing global temperatures result in considerable economic losses in M. japonicus farming. In the present study, to select genetically improved animals for the sustainable development of the Kuruma prawn industry, a high-resolution genetic linkage map and quantitative trait locus (QTL) identification were performed using the RAD technology. The maternal map contained 5849 SNP markers and spanned 3127.23 cM, with an average marker interval of 0.535 cM. Instead, the paternal map contained 3927 SNP markers and spanned 3326.19 cM, with an average marker interval of 0.847 cM. The consensus map contained 9289 SNP markers and spanned 3610.90 cM, with an average marker interval of 0.388 cM and coverage of 99.06 % of the genome. The markers were grouped into 41 linkage groups in the maps. Significantly, negative correlation was detected between high-temperature tolerance (UTT) and body weight (BW). The QTL mapping revealed 129 significant QTL loci for UTT and four significant QTL loci for BW at the genome-wide significance threshold. Among these QTLs, 129 overlapped with linked SNPs, and the remaining four were located in regions between contiguous SNPs. They explained the total phenotypic variance ranging from 8.9 to 12.4 %. Because of a significantly negative correlation between growth and high-temperature tolerance, we demonstrate that this high-resolution linkage map and QTLs would be useful for further marker-assisted selection in the genetic improvement of M. japonicus.
authors.affiliation: Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071, China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Lu
authors.affiliation: Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071, China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Luan
authors.affiliation: Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071, China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
authors.forename: Long Yang
authors.initials: LY
authors.lastname: Hu
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Shanghai LingEn Biological Technology Co., LTD, Shanghai, 201800, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Sheng Ping
authors.initials: SP
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071, China. kongjie@ysfri.ac.cn.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. kongjie@ysfri.ac.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Kong
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D005260:Female; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D006358:Hot Temperature; D008297:Male; D033561:Penaeidae / Q000235:genetics / Q000254:growth & development*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA / Q000379:methods*; D013315:Stress, Psychological
keywords: Body weight; High temperature resistance; High-resolution linkage mapping; Marsupenaeus japonicus; QTL mapping; RAD; SNP makers