title: Genome-Wide Search for Host Association Factors during Ovine Progressive Pneumonia Virus Infection.
abstract: Ovine progressive pneumonia virus (OPPV) is an important virus that causes serious diseases in sheep and goats with a prevalence of 36% in the USA. Although OPPV was discovered more than half of a century ago, little is known about the infection and pathogenesis of this virus. In this report, we used RNA-seq technology to conduct a genome-wide probe for cellular factors that are associated with OPPV infection. A total of approximately 22,000 goat host genes were detected of which 657 were found to have been significantly up-regulated and 889 down-regulated at 12 hours post-infection. In addition to previously known restriction factors from other viral infections, a number of factors which may be specific for OPPV infection were uncovered. The data from this RNA-seq study will be helpful in our understanding of OPPV infection, and also for further study in the prevention and intervention of this viral disease.
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.|School of Veterinary Medicine and Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Jesse
authors.initials: J
authors.lastname: Thompson
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Fangrui
authors.initials: F
authors.lastname: Ma
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.|School of Veterinary Medicine and Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Meghan
authors.initials: M
authors.lastname: Quinn
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.|School of Veterinary Medicine and Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Shi-Hua
authors.initials: SH
authors.lastname: Xiang
mesh_terms: D000818:Animals; D002460:Cell Line; D018450:Disease Progression; D023281:Genomics; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D017423:Sequence Analysis, RNA; D012756:Sheep / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D012757:Sheep Diseases / Q000821:virology*; D057074:Virus Release; D014779:Virus Replication; D014790:Visna-maedi virus / Q000502:physiology*
chemical_list: D008565:Membrane Proteins