title: Role of the Cys154Arg Substitution in Ribosomal Protein L3 in Oxazolidinone Resistance in Mycobacterium tuberculosis.
abstract: We expressed the wild-type rplC and mutated rplC (Cys154Arg) genes, respectively, in Mycobacterium tuberculosis H37Ra and H37Rv in an attempt to delineate the role of rplC (Cys154Arg) regarding oxazolidinone resistance. An increase of the MICs of linezolid (LZD) and sutezolid (PNU-100480, PNU) against the recombinant mycobacteria with overexpressed rplC mutation (Cys154Arg) was found, suggesting the rplC gene is a determinant of bacillary susceptibilities to LZD and PNU.
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China.
authors.forename: Gaëlle Guiewi
authors.initials: GG
authors.lastname: Makafe
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China School of Life Sciences, Anhui University, Hefei, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, The Guangzhou Chest Hospital, Guangzhou, China.
authors.forename: Yaoju
authors.initials: Y
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China.
authors.forename: Mugweru
authors.initials: M
authors.lastname: Julius
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China.
authors.forename: Moses M
authors.initials: MM
authors.lastname: Njire
authors.affiliation: State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, The Guangzhou Chest Hospital, Guangzhou, China.
authors.forename: Xingshan
authors.initials: X
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China School of Life Sciences, University of Science and Technology of China, Hefei, China.
authors.forename: Tianzhou
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China School of Life Sciences, Anhui University, Hefei, China.
authors.forename: Bangxing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Langfang Yanjing Vocational and Technical College, Langfang, China Forensic Medical Center, University of Maryland, Baltimore, Maryland, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.affiliation: State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, The Guangzhou Chest Hospital, Guangzhou, China.
authors.forename: Shouyong
authors.initials: S
authors.lastname: Tan
authors.affiliation: School of Life Sciences, Anhui University, Hefei, China.
authors.forename: Buchang
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Wing Wai
authors.initials: WW
authors.lastname: Yew
authors.affiliation: School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: Gyanu
authors.initials: G
authors.lastname: Lamichhane
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences (CAS), Guangzhou, China zhang_tianyu@gibh.ac.cn.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D000069349:Linezolid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D023303:Oxazolidinones / Q000494:pharmacology*; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000995:Antitubercular Agents; D023303:Oxazolidinones; C543015:PNU-100480; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; D000069349:Linezolid