title: SOX2 inhibits metastasis in gastric cancer.
abstract: OBJECTIVE
  To investigate the potential role of SOX2 in gastric cancer (GC) metastasis.
  
  
  METHODS
  The SOX2 expression was detected using immunohistochemistry on a GC tissue microarray. The correlations of SOX2 expression with clinicopathological factors and 5-year survival were evaluated. To test the role of SOX2 in inhibiting GC metastasis, the cell transwell assay was performed. Real-time PCR and Western blot were used to explore the possible mechanism that SOX2 inhibits GC metastasis.
  
  
  RESULTS
  In the present study, SOX2 expression was downregulated in GC tissues when compared to matching normal tissues. Moreover, patients with high SOX2 expression in cancerous tissues had less lymph node metastasis and better treatment outcome. At the subcellular level, SOX2 inhibited the GC cell migration and invasion by upregulating p21 expression. Moreover, SOX2 was determined to associate with the nuclear p21 expression. GC patients with high SOX2 and nuclear p21 expression had synergistically less lymph node metastasis and the better overall survival.
  
  
  CONCLUSIONS
  Our results suggest that SOX2 is a promising and favorable metastatic biomarker for GC.
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China.|School of Public Health, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, 221002, People's Republic of China.
authors.forename: Yansu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China.|School of Public Health, Xuzhou Medical College, 209 Tongshan Road, Xuzhou, 221002, People's Republic of China.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China.
authors.forename: Minjuan
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pathology, Nantong Cancer Hospital, 30 North Tongyang Road, Pingchao, Nantong, 226361, Jiangsu Province, People's Republic of China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Nantong Cancer Hospital, 30 North Tongyang Road, Pingchao, Nantong, 226361, Jiangsu Province, People's Republic of China.
authors.forename: Song
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Li
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, People's Republic of China. 101011816@seu.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Molecular Cell Biology and Toxicology, Cancer Center, School of Public Health, Nanjing Medical University, 101 Longmian Ave, Nanjing, 211166, People's Republic of China. drjwzhou@126.com.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D055748:SOXB1 Transcription Factors / Q000502:physiology*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors
keywords: Gastric cancer; Metastasis; Prognosis; SOX2; p21