title: Rapid screening and identification of phenolic antioxidants in Hydrocotyle sibthorpioides Lam. by UPLC-ESI-MS/MS.
abstract: The aim of the study was to identify the phenolic compounds present in Hydrocotyle sibthorpioides (HS), Centella asiatica (CA) and Amaranthus viridis (AV) extracts and investigate their respective antioxidant activities. Herein, an ultra-high pressure liquid chromatography-mass spectrometer (UPLC-MS/MS) analytical method has been developed for the separation, and systematic characterization of the phenolic compounds in HS, CA and AV extracts and was compared along with ten standard phenolic compounds. Additionally, in vitro antioxidant activity of the phenolic compounds was also determined. The HS extract revealed excellent antioxidant activity such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (IC50=19.7 ± 1.2 μg/mL), total reduction capability (0.169 ± 0.003 at 100 μg/mL), nitric oxide radical scavenging activity (IC50=39.33 ± 3.2 μg/mL), metal chelating activity (IC50=56.51 ± 3.6 μg/mL) and inhibition of lipid peroxidation (IC50=12.34 ± 2.3 μg/mL) as compared to CA and AV extracts. Furthermore, catechin, epicatechin, quercetin and chlorogenic acid were found to be the major components responsible for the antioxidant activity of the HS extract as evidenced from UPLC-MS/MS. Taken together, this study demonstrates the promising antioxidant properties of the HS extract, which can further be utilized in various pharmaceutical, food, and agricultural applications.
authors.affiliation: Biochemistry Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: Sima
authors.initials: S
authors.lastname: Kumari
authors.affiliation: Drug Discovery Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: R
authors.initials: R
authors.lastname: Elancheran
authors.affiliation: Drug Discovery Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India.
authors.forename: Jibon
authors.initials: J
authors.lastname: Kotoky
authors.affiliation: Biochemistry Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati 781035, Assam, India. Electronic address: rajiasst@gmail.com.
authors.forename: Rajlakshmi
authors.initials: R
authors.lastname: Devi
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001713:Biphenyl Compounds / Q000032:analysis; D002392:Catechin / Q000032:analysis; D028041:Centella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D062385:Hydroxybenzoates / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D010851:Picrates / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis; D011794:Quercetin / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D062385:Hydroxybenzoates; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D002392:Catechin; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl; C017616:phenolic acid
keywords: (+)-Catechin (PubChem CID: 107957); (–)-Epicatechin (PubChem CID: 72276); Antioxidants; Apigenin (PubChem CID: 5280443); Biochanin A (PubChem CID: 5280373); Caffeine (PubChem CID: 2519); Chlorogenic acid (PubChem CID: 1794427); Flavonoids; Phenolic acids; Polyphenols; Quercetin (PubChem CID: 5280343); Rosmarinic acid (PubChem CID: 5281792); Sinapic acid (PubChem CID: 637775); Trans-cinnamic acid (PubChem CID: 444539); Tyrosol (PubChem CID: 10393); UHPLC–MS