title: Fractionation of sheep cheese whey by a scalable method to sequentially isolate bioactive proteins.
abstract: This study reports a procedure for the simultaneous purification of glyco(caseino)macropeptide, immunoglobulin, lactoperoxidase, lactoferrin, α-lactalbumin and β-lactoglobulin from sheep cheese sweet whey, an under-utilized by-product of cheese manufacture generated by an emerging sheep dairy industry in New Zealand. These proteins have recognized value in the nutrition, biomedical and health-promoting supplements industries. A sequential fractionation procedure using economical anion and cation exchange chromatography on HiTrap resins was evaluated. The whey protein fractionation is performed under mild conditions, requires only the adjustment of pH between ion exchange chromatography steps, does not require buffer exchange and uses minimal amounts of chemicals. The purity of the whey protein fractions generated were analyzed by reversed phase-high performance liquid chromatography and the identity of the proteins was confirmed by mass spectrometry. This scalable procedure demonstrates that several proteins of recognized value can be fractionated in reasonable yield and purity from sheep cheese whey in one streamlined process.
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand. Electronic address: jodi.pilbrow@otago.ac.nz.
authors.forename: Jodi
authors.initials: J
authors.lastname: Pilbrow
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand.
authors.forename: Alaa El-Din A
authors.initials: AEA
authors.lastname: Bekhit
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: Alan
authors.initials: A
authors.lastname: Carne
mesh_terms: D000818:Animals; D002611:Cheese / Q000032:analysis*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007768:Lactalbumin / Q000302:isolation & purification*; D007781:Lactoferrin / Q000302:isolation & purification*; D007782:Lactoglobulins / Q000302:isolation & purification*; D007784:Lactoperoxidase / Q000302:isolation & purification*; D013058:Mass Spectrometry; D009520:New Zealand; D009753:Nutritive Value; D012756:Sheep; D000067796:Whey / Q000737:chemistry
chemical_list: D007782:Lactoglobulins; D007768:Lactalbumin; D007784:Lactoperoxidase; D007781:Lactoferrin
keywords: Chromatography; Ion-exchange; Sheep cheese whey protein