title: Human limbal mesenchymal stem cells express ABCB5 and can grow on amniotic membrane.
abstract: OBJECTIVE
  To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings.
  
  
  METHODS
  Cells were isolated from corneoscleral rings and cultured in a mesenchymal stem cell (MSC)-selective media and examined for differentiation, phenotyping and characterization.
  
  
  RESULTS
  LMSCs were capable of trilineage differentiation, adhered to tissue culture plastic, expressed HLA class I and cell surface antigens associated with human MSC while having no/low expression of HLA class II and negative hematopoietic lineage markers. They were capable for CXCL12-mediated cellular migration. LMSCs adhered, proliferated on amniotic membrane and expressed the common putative limbal stem cell markers.
  
  
  CONCLUSIONS
  Limbal-derived MSC exhibited plasticity, could maintain limbal markers expression and demonstrated viable growth on amniotic membrane.
authors.affiliation: Institute of Genetic Medicine, Faculty of Medical Sciences, Newcastle University, Newcastle Upon-Tyne, NE1 3BZ, UK.|Advanced Medical & Dental Institute, Universiti Sains Malaysia, 13200 Pulau Pinang, Malaysia.
authors.forename: Bakiah
authors.initials: B
authors.lastname: Shaharuddin
authors.affiliation: Institute of Genetic Medicine, Faculty of Medical Sciences, Newcastle University, Newcastle Upon-Tyne, NE1 3BZ, UK.
authors.forename: Charles
authors.initials: C
authors.lastname: Osei-Bempong
authors.affiliation: St Paul's Eye Unit, Royal Liverpool University Hospital, Prescot Street, Liverpool, L7 8XP, UK.|Department of Eye & Vision Sciences, Institute of Ageing & Chronic Disease, Faculty of Health & Life Sciences, University of Liverpool, Liverpool, L69 3GA, UK.
authors.forename: Sajjad
authors.initials: S
authors.lastname: Ahmad
authors.affiliation: Tissue Development Laboratory, NHS Blood & Transplant, Estuary Banks, Liverpool, L24 8RB, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Rooney
authors.affiliation: Institute of Cellular Medicine, Newcastle University, Newcastle Upon-Tyne, NE2 4HH, UK.
authors.forename: Simi
authors.initials: S
authors.lastname: Ali
authors.affiliation: Department of Musculoskeletal Biology Group I, Institute of Ageing & Chronic Disease, Faculty of Health & Life Sciences, University of Liverpool, Leahurst Campus, Chester High Road, Neston, Cheshire, CH64 7TE, UK.
authors.forename: Rachel
authors.initials: R
authors.lastname: Oldershaw
authors.affiliation: Institute of Genetic Medicine, Faculty of Medical Sciences, Newcastle University, Newcastle Upon-Tyne, NE1 3BZ, UK.
authors.forename: Annette
authors.initials: A
authors.lastname: Meeson
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis*; D000650:Amnion; D054377:Chemokine CXCL12 / Q000096:biosynthesis; D005260:Female; D005786:Gene Expression Regulation; D006680:HLA Antigens / Q000096:biosynthesis; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C480744:ABCB5 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D006680:HLA Antigens
keywords: ABCB5; amniotic membrane; limbal mesenchymal stem cells; limbal stem cell deficiency; limbal stem cells