title: Membrane Contact Sites: Complex Zones for Membrane Association and Lipid Exchange.
abstract: Lipid transport between membranes within cells involves vesicle and protein carriers, but as agents of nonvesicular lipid transfer, the role of membrane contact sites has received increasing attention. As zones for lipid metabolism and exchange, various membrane contact sites mediate direct associations between different organelles. In particular, membrane contact sites linking the plasma membrane (PM) and the endoplasmic reticulum (ER) represent important regulators of lipid and ion transfer. In yeast, cortical ER is stapled to the PM through membrane-tethering proteins, which establish a direct connection between the membranes. In this review, we consider passive and facilitated models for lipid transfer at PM-ER contact sites. Besides the tethering proteins, we examine the roles of an additional repertoire of lipid and protein regulators that prime and propagate PM-ER membrane association. We conclude that instead of being simple mediators of membrane association, regulatory components of membrane contact sites have complex and multilayered functions.
authors.affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.
authors.forename: Evan
authors.initials: E
authors.lastname: Quon
authors.affiliation: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.; Centre for Cell Biology, Development, and Disease, Simon Fraser University, Burnaby, BC, Canada.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Beh
keywords: endoplasmic reticulum; lipid transfer proteins; membrane contact sites; membrane lipids; membrane-tethering proteins; nonvesicular transport; plasma membrane