title: Reclamation of DPK hydrocarbon polluted agricultural soil using a selected bulking agent.
abstract: In the present study, laboratory scale bioremediation of dual purpose kerosene (DPK) hydrocarbon polluted soil using bulking agent (saw dust) was carried out. The effect of different parameters such as total petroleum hydrocarbon (TPH), dehydrogenase activity (DHase) and pH on bioremediation performance were evaluated. Studied parameters such as microbial dynamics, percentage degradation (95.20%), DHase (8.20 ± 0.43) were found to be higher in saw dust amended system and significantly differed with control at p < 0.05. Experimental data adequately fitted the first order kinetic thus, generated r(2) values (0.966), first order degradation constant (0.659 d(-1)), and degradation half-life t1/2 = ln2/k (1.05 d). Micrococcus luteus, Bacillus sp., Rhizopus arrhizus and Aspergillus sp. were isolated from the study. The use of saw dust as bulking agent greatly increased biodegradation rate and resulted in effective DPK hydrocarbon clean up. Therefore, saw dust could serve as an effective biostimulant towards improved bioremediation of hydrocarbon polluted environment.
authors.affiliation: Department of Applied Microbiology and Brewing, Faculty of Biosciences, NnamdiAzikiwe University PMB, 5025, Awka, Anambra State, Nigeria. Electronic address: smetchie@yahoo.com.
authors.forename: Amechi S
authors.initials: AS
authors.lastname: Nwankwegu
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
authors.forename: Chukwudi O
authors.initials: CO
authors.lastname: Onwosi
authors.affiliation: Department of Applied Microbiology and Brewing, Faculty of Biosciences, NnamdiAzikiwe University PMB, 5025, Awka, Anambra State, Nigeria.
authors.forename: Michael U
authors.initials: MU
authors.lastname: Orji
authors.affiliation: Department of Applied Microbiology and Brewing, Faculty of Biosciences, NnamdiAzikiwe University PMB, 5025, Awka, Anambra State, Nigeria.
authors.forename: Chika G
authors.initials: CG
authors.lastname: Anaukwu
authors.affiliation: Department of Applied Microbiology and Brewing, Faculty of Biosciences, NnamdiAzikiwe University PMB, 5025, Awka, Anambra State, Nigeria.
authors.forename: Uchenna C
authors.initials: UC
authors.lastname: Okafor
authors.affiliation: Department of Food Science, Ebonyi State University EBSU, Abakaliki, Ebonyi State, Nigeria.
authors.forename: Fidelis
authors.initials: F
authors.lastname: Azi
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Martins
mesh_terms: D000383:Agriculture; D001407:Bacillus / Q000378:metabolism; D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006207:Half-Life; D006838:Hydrocarbons / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007648:Kerosene; D016982:Micrococcus luteus / Q000378:metabolism; D009549:Nigeria; D010578:Petroleum; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D006838:Hydrocarbons; D007648:Kerosene; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants
keywords: Bioremediation; Dual purpose kerosene; Kinetics; Saw dust; Total petroleum hydrocarbon