title: Influence of different prebiotics and mode of their administration on broiler chicken performance.
abstract: In the post-antibiotics era, prebiotics are proposed as alternatives to antibiotic growth promoters in poultry production. The goal of this study was to compare in ovo method of prebiotic delivery with in-water supplementation and with both methods combined (in ovo+in-water) in broiler chickens. Two trials were conducted. Trial 1 was carried out to optimize the doses of two prebiotics, DN (DiNovo®, extract of beta-glucans) and BI (Bi2tos, trans-galactooligosaccharides), for in ovo delivery. The estimated parameters were hatchability and bacteriological status of the newly hatched chicks. Prebiotics were dissolved in 0.2 ml of physiological saline, at the doses: 0.18, 0.88, 3.5 and 7.0 mg/embryo; control group (C) was injected in ovo with 0.2 ml of physiological saline. Trial 2 was conducted to evaluate effects of different prebiotics (DN, BI and raffinose family oligosaccharides (RFO)) delivered in ovo, in-water and in a combined way (in ovo+in-water) on broiler chickens performance. The results of the Trial 1 indicated that the optimal dose of DN and BI prebiotics delivered in ovo, that did not reduce chicks' hatchability, was 0.88 mg/embryo (DN) and 3.5 mg/embryo (BI). Both prebiotics numerically increased number of lactobacilli and bifidobacteria in chicken feces (P>0.05). In Trial 2, all prebiotics (DN, BI and RFO) significantly increased BW gain compared with the C group (P<0.05), especially during the first 21 days of life. However, feed intake and feed conversion ratio were increased upon prebiotics delivery irrespective of method used. Injection of prebiotics in ovo combined with in-water supplementation did not express synergistic effects on broilers performance compared with in ovo injection only. Taken together, those results confirm that single in ovo prebiotics injection into the chicken embryo can successfully replace prolonged in-water supplementation post hatching.
authors.affiliation: 1Department of Animal Biochemistry and Biotechnology,UTP University of Science and Technology,Mazowiecka 28,85-084 Bydgoszcz,Poland.
authors.forename: M
authors.initials: M
authors.lastname: Bednarczyk
authors.affiliation: 1Department of Animal Biochemistry and Biotechnology,UTP University of Science and Technology,Mazowiecka 28,85-084 Bydgoszcz,Poland.
authors.forename: K
authors.initials: K
authors.lastname: Stadnicka
authors.affiliation: 1Department of Animal Biochemistry and Biotechnology,UTP University of Science and Technology,Mazowiecka 28,85-084 Bydgoszcz,Poland.
authors.forename: I
authors.initials: I
authors.lastname: Kozłowska
authors.affiliation: 2Department of Agricultural, Environmental and Food Sciences,University of Molise,Via F. De Sanctis,86100 Campobasso,Italy.
authors.forename: C
authors.initials: C
authors.lastname: Abiuso
authors.affiliation: 2Department of Agricultural, Environmental and Food Sciences,University of Molise,Via F. De Sanctis,86100 Campobasso,Italy.
authors.forename: S
authors.initials: S
authors.lastname: Tavaniello
authors.affiliation: 1Department of Animal Biochemistry and Biotechnology,UTP University of Science and Technology,Mazowiecka 28,85-084 Bydgoszcz,Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dankowiakowska
authors.affiliation: 1Department of Animal Biochemistry and Biotechnology,UTP University of Science and Technology,Mazowiecka 28,85-084 Bydgoszcz,Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sławińska
authors.affiliation: 2Department of Agricultural, Environmental and Food Sciences,University of Molise,Via F. De Sanctis,86100 Campobasso,Italy.
authors.forename: G
authors.initials: G
authors.lastname: Maiorano
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed / Q000032:analysis; D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000254:growth & development / Q000382:microbiology / Q000502:physiology*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000382:microbiology; D007267:Injections / Q000662:veterinary; D009844:Oligosaccharides / Q000008:administration & dosage*; D056692:Prebiotics / Q000008:administration & dosage*; D011897:Random Allocation; D015430:Weight Gain; D047071:beta-Glucans / Q000008:administration & dosage*
chemical_list: D009844:Oligosaccharides; D056692:Prebiotics; D047071:beta-Glucans
keywords: hatchability; in ovo; microflora; performance traits; prebiotics