title: Three-dimensional poly (ε-caprolactone)/hydroxyapatite/collagen scaffolds incorporating bone marrow mesenchymal stem cells for the repair of bone defects.
abstract: We previously demonstrated that three-dimensional (3D) hydroxyapatite (HAP)-collagen (COL)-coated poly(ε-caprolactone) (PCL) scaffolds (HAP-COL-PCL) possess appropriate nano-structures, surface roughness, and nutrients, providing a favorable environment for osteogenesis. However, the effect of using 3D HAP-COL-PCL scaffolds incorporating BMSCs for the repair of bone defects in rats has been not evaluated. 3D PCL scaffolds coated with HAP, collagen or HAP/COL and incorporating BMSCs were implanted into calvarial defects. At 12 weeks after surgery, the rats were sacrificed and crania were harvested to assess the bone defect repair using microcomputed tomography (micro-CT), histology, immunohistochemistry and sequential fluorescent labeling analysis. 3D micro-CT reconstructed images and quantitative analysis showed that HAP-COL-PCL groups possessed better bone-forming capacity than HAP-PCL groups or COL-PCL groups. Fluorescent labeling analysis revealed the percentage of tetracycline labeling, alizarin red labeling, and calcein labeling in HAP-COL-PCL groups were all greater than in the other two groups (P < 0.05), and the result was confirmed by immunohistochemical staining and histological analysis of bone regeneration. This study demonstrates that 3D HAP-COL-PCL scaffolds incorporating BMSCs markedly enhance bone regeneration of bone defects in rats.
authors.affiliation: Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China. These authors contributed equally.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.forename: Yinjun
authors.initials: Y
authors.lastname: Huang
authors.forename: Dan
authors.initials: D
authors.lastname: Han
authors.forename: Jieyuan
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Cao
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Jin
authors.forename: Jinghuan
authors.initials: J
authors.lastname: Huang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003094:Collagen; D017886:Durapatite; D005260:Female; D021621:Imaging, Three-Dimensional; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010012:Osteogenesis; D011091:Polyesters; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen; D017886:Durapatite