title: Platelet-Rich Plasma in Grafted Maxillae: Growth Factor Quantification and Dynamic Histomorphometric Evaluation.
abstract: OBJECTIVE
  Validation of platelet-rich plasma (PRP) system and assessing its enhancing effect on the healing of iliac crest grafts before implant placement.
  
  
  METHODS
  Patients randomly allocated to test (n = 13) and control (n = 9) groups. Iliac crest grafts were mixed with PRP in the test group. Tetracycline labeling preceded implant placement. Bone samples were harvested for histomorphometrical analysis. Platelet and growth factor quantifications were performed.
  
  
  METHODS
  Data were analyzed using SPSS software package. Independent t test was used and statistical significance was set at 5%.
  
  
  RESULTS
  The PRP group showed significantly higher platelet counts, PDGF-BB, and TGF-β1 concentrations. Tendency to higher volume of woven bone was observed in the PRP group (13 ± 11 vs 4 ± 6, P = 0.1). Histomorphometry showed increased seam separation in the PRP group (8.8 ± 9 μm vs 1.5 ± 3 μm, P = 0.039). Remodeling activity was higher in PRP-woven bone sections and comparable in trabecular sections.
  
  
  CONCLUSIONS
  PRP significantly increased platelet and growth factor concentrations and was of possible enhancing effect on the rate of bone formation at 3 to 4 months of grafting. The clinical significance of this enhancement is yet to be established.
authors.affiliation: *Lecturer of Oral and Maxillofacial Surgery, Department of Oral and Maxillofacial Surgery, Minia University, Minia, Egypt. †Honorary Senior Lecturer of Oral and Maxillofacial Surgery, Cardiff University, Cardiff, UK. ‡Senior Clinical Scientist, Department of Biochemistry, Central Manchester University Hospitals, Manchester, UK. §Head of School of Dentistry and Professor of Oral and Maxillofacial Surgery, The University of Manchester, Manchester, UK.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Badr
authors.forename: Richard
authors.initials: R
authors.lastname: Oliver
authors.forename: Philip
authors.initials: P
authors.lastname: Pemberton
authors.forename: Paul
authors.initials: P
authors.lastname: Coulthard
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000077214:Becaplermin; D001846:Bone Development / Q000502:physiology; D016025:Bone Transplantation / Q000379:methods*; D005260:Female; D006801:Humans; D007085:Ilium / Q000473:pathology / Q000601:surgery*; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis* / Q000502:physiology; D008297:Male; D008437:Maxilla / Q000473:pathology / Q000601:surgery*; D008875:Middle Aged; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000737:chemistry / Q000502:physiology; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis / Q000502:physiology; D059546:Sinus Floor Augmentation / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000502:physiology; D055815:Young Adult
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin