title: Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster.
abstract: Lamin family proteins are structural components of a filamentous framework, the nuclear lamina (NL), underlying the inner membrane of nuclear envelope. The NL not only plays a role in nucleus mechanical support and nuclear shaping, but is also involved in many cellular processes including DNA replication, gene expression and chromatin positioning. Spermatogenesis is a very complex differentiation process in which each stage is characterized by nuclear architecture dramatic changes, from the early mitotic stage to the sperm differentiation final stage. Nevertheless, very few data are present in the literature on the NL behavior during this process. Here we show the first and complete description of NL behavior during meiosis and spermatogenesis in Drosophila melanogaster. By confocal imaging, we characterized the NL modifications from mitotic stages, through meiotic divisions to sperm differentiation with an anti-laminDm0 antibody against the major component of the Drosophila NL. We observed that continuous changes in the NL structure occurred in parallel with chromatin reorganization throughout the whole process and that meiotic divisions occurred in a closed context. Finally, we analyzed NL in solofuso meiotic mutant, where chromatin segregation is severely affected, and found the strict correlation between the presence of chromatin and that of NL.
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Fabbretti
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Iannetti
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Guglielmi
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Susanna
authors.initials: S
authors.lastname: Perconti
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Evangelistella
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Proietti De Santis
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bongiorni
authors.affiliation: Department of Ecology and Biology, Università della Tuscia, Viterbo, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Prantera
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D004331:Drosophila melanogaster; D034882:Lamins / Q000378:metabolism; D008297:Male; D008540:Meiosis / Q000502:physiology*; D018613:Microscopy, Confocal / Q000379:methods*; D009685:Nuclear Envelope / Q000378:metabolism; D034881:Nuclear Lamina / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*
chemical_list: D034882:Lamins