title: miR-522 contributes to cell proliferation of hepatocellular carcinoma by targeting DKK1 and SFRP2.
abstract: The morbidity and mortality of hepatocellular carcinoma (HCC) is very high, finding new therapeutic targets are critical for HCC treatment. miR-522 has been demonstrated to be upregulated in HCC tissues, but its role in HCC progression remains to be elucidated. In this report, we found miR-522 was upregulated in HCC cells and tissues, miR-522 overexpression promoted cell proliferation, colony formation, and cell cycle progression, whereas knockdown of miR-522 reduced these effects. We also analyzed the expression of several key cell cycle regulatory proteins and found overexpression of miR-522-inhibited cell cycle inhibitors p21 and p27 expression and enhanced cyclin D1 expression and the level of Rb phosphorylation, vice versa. These suggested miR-522-accelerated G1/S transition. DKK1 (dickkopf-1) and SFRP2 (secreted frizzled-related protein 2) were the targets of miR-522, their expression was inversely with miR-522 in HCC tissues. DKK1 and SFRP2 the antagonists of Wnt signaling, suggesting miR-522-promoted HCC progression through activating Wnt signaling. miR-522 might be a valuable target for HCC therapy.
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China.
authors.forename: Meiyuan
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China.
authors.forename: Se
authors.initials: S
authors.lastname: Tian
authors.affiliation: Department of Hepatic-Biliary-Pancreatic Surgery, Hubei Cancer Hospital, 116 Zhuodaoquan south road, Hongshan district, Wuhan, 430079, Hubei, People's Republic of China. jjx731003@163.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China. chengyisun2014@163.com.
authors.forename: Chengyi
authors.initials: C
authors.lastname: Sun
mesh_terms: D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D035683:MicroRNAs; C501126:DKK1 protein, human; C560599:MIRN522 microRNA, human; C109618:SFRP2 protein, human; D000094742:Secreted Frizzled-Related Proteins
keywords: Cell proliferation; DKK1 and SFRP2; HCC; miR-522