title: Application of DNA barcoding for identifying forensically relevant Diptera from northern Thailand.
abstract: In recent decades, forensic entomology has become a useful tool in criminal investigations all over the world. Species-specific identification of flies plays an important role in this field and is obligatory for accurate calculation of the post-mortem interval. However, not all important colonizers of a corpse can be identified by common morphological keys. Due to similar morphology and the lack of keys for some taxa, especially for immature stages, DNA barcoding has become more popular during the last recent years. This development is particularly important for countries like Thailand, in which forensic entomology is a newly developing research area and which faces several challenges such as a high biodiversity of fly species. The most commonly used barcoding region in forensic entomology, the mitochondrial cytochrome oxidase subunit 1 (coI) gene, as well as a 1000-bp-long region of the 28S nuclear rRNA gene, was used to analyze and establish the molecular barcodes of 13 different species of flies of forensic relevance in northern Thailand.
authors.affiliation: Institute of Legal Medicine, Forensic Biology/Entomology, Kennedyallee 104, 60596, Frankfurt am Main, Germany. zajac@med.uni-frankfurt.de.
authors.forename: Barbara Karolina
authors.initials: BK
authors.lastname: Zajac
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors.forename: Narin
authors.initials: N
authors.lastname: Sontigun
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Wannasan
authors.affiliation: Institute of Legal Medicine, Forensic Biology/Entomology, Kennedyallee 104, 60596, Frankfurt am Main, Germany.
authors.forename: Marcel A
authors.initials: MA
authors.lastname: Verhoff
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
authors.forename: Kabkaew
authors.initials: K
authors.lastname: Sukontason
authors.affiliation: Institute of Legal Medicine, Forensic Biology/Entomology, Kennedyallee 104, 60596, Frankfurt am Main, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Amendt
authors.affiliation: Institute of Legal Medicine, Forensic Biology/Entomology, Kennedyallee 104, 60596, Frankfurt am Main, Germany.
authors.forename: Richard
authors.initials: R
authors.lastname: Zehner
mesh_terms: D000818:Animals; D001344:Autopsy / Q000379:methods; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004175:Diptera / Q000145:classification* / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004772:Entomology / Q000379:methods; D044707:Forensic Sciences / Q000379:methods; D016133:Polymerase Chain Reaction; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D012339:RNA, Ribosomal, 28S; D003576:Electron Transport Complex IV
keywords: 28S; Barcoding; Forensic entomology; Identification success; Thailand; coI