title: Characterization of Polyphenols from Lycium ruthenicum Fruit by UPLC-Q-TOF/MS(E) and Their Antioxidant Activity in Caco-2 Cells.
abstract: The fruit of Lycium ruthenicum Murr. (LRF) has long been used in folk medicine. Nevertheless, detailed information related to its polyphenol compositions remains scarce. In this study, we confirmed that the total phenolic and anthocyanin contents of LRF fruit extracts (LRFEs) were 4906.5 ± 60.6 mg of gallic acid equivalents/100 g DW and 787.6 ± 34.1 mg of cyanindin-3-glucoside equivalents/100 g DW, respectively. A characterization of LRFEs was performed by ultrahigh performance liquid chromatography/quadrupole time-of-flight mass spectrometry using an MS(E) data acquisition. A total of 26 polyphenols were tentatively identified, of which 19 represent the first reports of these polyphenols in LRFEs. Furthermore, the cellular antioxidant array showed that LRFEs could protect Caco-2 cells against H2O2-induced oxidative damage based on microscopic fluorometric imaging.
authors.affiliation: Key Laboratory of Food Bio-technology, School of Food and Bioengineering, Xihua University , Chengdu, 610039, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences , Beijing 100193, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Lv
authors.affiliation: Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences , Beijing 100193, China.
authors.forename: Fengzhong
authors.initials: F
authors.lastname: Wang
authors.affiliation: Xi'an Manareco New Materials Co. Ltd. , Xi'an, 710086, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000494:pharmacology*; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects*; D032305:Lycium; D013058:Mass Spectrometry / Q000379:methods*; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D059808:Polyphenols; D006861:Hydrogen Peroxide
keywords: Lycium ruthenicum Murr.; MSE; antioxidant activity; polyphenols