title: Establishment of serological test to detect antibody against ferret coronavirus.
abstract: Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method for detection of FRCoV infection. Many serum samples collected from ferrets recognized both a.a. 1-179 and a.a. 180-374 of the N protein, but two serum samples did not a.a. 180-374 of the N protein. This different reactivity was also confirmed by immunoblot analysis using the serum from the ferret.Therefore, the a.a. 1-179 of the N protein was used as an ELISA antigen. Serological test was carried out using sera or plasma of ferrets in Japan. Surprisingly, 89% ferrets in Japan had been infected with FRCoV. These results indicated that our established ELISA using a.a. 1-179 of the N protein is useful for detection of antibody to FRCoV for diagnosis and seroepidemiology of FRCoV infection.
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Minami
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Terada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Masaki
authors.initials: M
authors.lastname: Takizawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Onuma
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ota
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ota
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Akabane
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tamukai
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Watanabe
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Naganuma
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kanagawa
authors.forename: Kaneichi
authors.initials: K
authors.lastname: Nakamura
authors.forename: Masanari
authors.initials: M
authors.lastname: Ohashi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takami
authors.forename: Yasutsugu
authors.initials: Y
authors.lastname: Miwa
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tanoue
authors.forename: Masao
authors.initials: M
authors.lastname: Ohwaki
authors.forename: Jouji
authors.initials: J
authors.lastname: Ohta
authors.forename: Yumi
authors.initials: Y
authors.lastname: Une
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D017934:Coronavirus / Q000276:immunology*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D005289:Ferrets / Q000821:virology*; D015151:Immunoblotting / Q000662:veterinary; D008297:Male
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral