title: Phenolic Composition and Evaluation of Methanol and Aqueous Extracts of Bitter Gourd (Momordica charantia L) Leaves on Angiotensin-I-Converting Enzyme and Some Pro-oxidant-Induced Lipid Peroxidation In Vitro.
abstract: This study sought to assess the effects of bitter gourd leaf extracts (methanol and aqueous) on enzyme linked with hypertension (angiotensin-I-converting enzyme and some pro-oxidants (iron sulfate, sodium nitroprusside, and cisplatin]-induced lipid peroxidation in rat kidney homogenates. Chromatographic analysis of the extract was done using high-performance liquid chromatography-diode array detection. The results revealed that methanol extract (IC50= 109.63 µg/mL) had significantly (P < .05) higher angiotensin-I converting enzyme inhibitory activity than aqueous extract (IC50= 182.95 µg/mL). Similarly, methanol extract had significantly (P < .05) higher inhibitory effect on Fe2+- and cisplatin-induced lipid peroxidation than aqueous extract. However, there was no significant (P > .05) difference in the inhibition of sodium nitroprusside-induced lipid peroxidation of both extracts. High-performance liquid chromatography-diode array detection analysis revealed the presence of quercetin, caffeic, gallic, and chlorogenic acids in the leaf. Thus, inhibition of angiotensin-I converting enzyme activity and the antioxidant properties of the extracts could be linked to the presence of phenolic phytochemicals.
authors.affiliation: Adekunle Ajasin University, Akungba Akoko, Ondo State, Nigeria Federal University of Technology, Akure, Nigeria.
authors.forename: Sidiqat A
authors.initials: SA
authors.lastname: Shodehinde
authors.affiliation: Federal University of Technology, Akure, Nigeria.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Adefegha
authors.affiliation: Federal University of Technology, Akure, Nigeria goboh2001@yahoo.com.
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.affiliation: Federal University of Technology, Akure, Nigeria.
authors.forename: Sunday I
authors.initials: SI
authors.lastname: Oyeleye
authors.affiliation: Federal University of Technology, Akure, Nigeria Federal Institute of Industrial Research, Oshodi, Nigeria tosinolasehinde26@yahoo.com.
authors.forename: Tosin A
authors.initials: TA
authors.lastname: Olasehinde
authors.affiliation: Federal University of Technology, Akure, Nigeria.
authors.forename: Emmem E
authors.initials: EE
authors.lastname: Nwanna
authors.affiliation: Federal University of Technology, Akure, Nigeria.
authors.forename: Bukola C
authors.initials: BC
authors.lastname: Adedayo
authors.affiliation: Federal University of Santa Maria, Santa Maria, Brazil.
authors.forename: Aline A
authors.initials: AA
authors.lastname: Boligon
keywords: angiotensin-1 converting enzyme; antihypertension; antioxidant; bitter gourd; lipid peroxidation; renoprotection