title: Attachment issues: kinetochore transformations and spindle checkpoint silencing.
abstract: Cell division culminates in the segregation of duplicated chromosomes in opposite directions prior to cellular fission. This process is guarded by the spindle assembly checkpoint (SAC), which prevents the anaphase of cell division until stable connections between spindle microtubules and the kinetochores of all chromosomes are established. The anaphase inhibitor is generated at unattached kinetochores and inhibitor production is prevented when microtubules are captured. Understanding the molecular changes in the kinetochore that are evoked by microtubule attachments is crucial for understanding the mechanisms of SAC signaling and silencing. Here, we highlight the most recent findings on these events, pinpoint some remaining mysteries, and argue for incorporating holistic views of kinetochore dynamics in order to understand SAC silencing.
authors.affiliation: Hubrecht Institute - KNAW (Royal Netherlands Academy of Arts and Sciences), Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
authors.forename: Banafsheh
authors.initials: B
authors.lastname: Etemad
authors.affiliation: Hubrecht Institute - KNAW (Royal Netherlands Academy of Arts and Sciences), Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; Cancer Genomics Netherlands, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands; Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG Utrecht, The Netherlands. Electronic address: g.kops@hubrecht.eu.
authors.forename: Geert J P L
authors.initials: GJ
authors.lastname: Kops
mesh_terms: D000705:Anaphase; D000818:Animals; D059447:Cell Cycle Checkpoints; D006801:Humans; D018386:Kinetochores / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000378:metabolism