title: Investigations of in vitro bioaccessibility from interesterified stearic and oleic acid-rich blends.
abstract: Interesterification was previously found to impact stearic acid absorption in a randomized cross-over study, when human volunteers consumed a 70 : 30 wt% high-oleic sunflower and canola stearin blend (NIE) compared to the same blend which had undergone either chemical (CIE) or enzymatic (EIE) interesterification. In this research, in vitro lipid digestion, bioaccessibility, and changes in undigested lipid composition and melting behavior of these same test fats were investigated using the dynamic, multi-compartmental TIM-1 digestion model and compared with the previous human study. Overall, TIM-1 bioaccessibility was higher with interesterification (p < 0.05). Oleic acid bioaccessibility was higher than stearic acid bioaccessibility for NIE, and vice versa for the interesterified blends (p < 0.05). Stearic acid was more concentrated in the undigested triacylglycerols (TAG) from NIE, corresponding to a relatively higher melting temperature of the undigested lipids. The results confirm the impact of TAG composition, fatty acid position and/or physical properties on lipid digestion. TIM-1 bioaccessibility was linearly correlated (R(2) = 0.8640) with postprandial serum TAG concentration in the human study. Therefore, the in vitro digestion model offered predictive insights related to the impacts of lipid interesterificaton on absorption.
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Road East, Guelph ON N1G 2W1 Canada. ajwright@uoguelph.ca.
authors.forename: S H
authors.initials: SH
authors.lastname: Thilakarathna
authors.affiliation: Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1 Canada and New Jersey Institute of Food, Nutrition, and Health, Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, NJ 08901, USA.
authors.forename: M
authors.initials: M
authors.lastname: Rogers
authors.affiliation: New Jersey Institute of Food, Nutrition, and Health, Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, NJ 08901, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Lan
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Road East, Guelph ON N1G 2W1 Canada. ajwright@uoguelph.ca.
authors.forename: S
authors.initials: S
authors.lastname: Huynh
authors.affiliation: Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1 Canada.
authors.forename: A G
authors.initials: AG
authors.lastname: Marangoni
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Road East, Guelph ON N1G 2W1 Canada. ajwright@uoguelph.ca.
authors.forename: L E
authors.initials: LE
authors.lastname: Robinson
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Road East, Guelph ON N1G 2W1 Canada. ajwright@uoguelph.ca.
authors.forename: A J
authors.initials: AJ
authors.lastname: Wright
mesh_terms: D000818:Animals; D001682:Biological Availability; D002417:Cattle; D018592:Cross-Over Studies; D004063:Digestion; D004951:Esterification; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism*; D000074262:Rapeseed Oil; D013229:Stearic Acids / Q000737:chemistry / Q000378:metabolism*; D000074242:Sunflower Oil; D013552:Swine
chemical_list: D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D013229:Stearic Acids; D000074242:Sunflower Oil; D019301:Oleic Acid; C031183:stearic acid