title: Polychlorinated biphenyl 126 exposure in L6 myotubes alters glucose metabolism: a pilot study.
abstract: Polychlorinated biphenyls (PCBs) are increasingly recognized as metabolic disruptors. Due to its mass, skeletal muscle is the major site of glucose disposal. While muscle mitochondrial dysfunction and oxidative stress have been shown to play a central role in metabolic disease development, no studies to date have investigated the effect of PCB exposure on muscle energy metabolism and oxidative stress. In this pilot study, we tested the effect of exposure to PCB126 in L6 myotubes (from 1 to 2500 nM for 24 h) on mitochondrial function, glucose metabolism, and oxidative stress. Exposure to PCB126 had no apparent effect on resting, maximal, and proton leak-dependent oxygen consumption rate in intact L6 myotubes. However, basal glucose uptake and glycolysis were inhibited by 20-30 % in L6 myotubes exposed to PCB126. Exposure to PCB126 did not appear to alter skeletal muscle anti-oxidant defense or oxidative stress. In conclusion, our study shows for the first time that exposure to a dioxin-like PCB adversely affects skeletal muscle glucose metabolism. Given the importance of skeletal muscle in the maintenance of glucose homeostasis, PCB126 could play an important role in the development of metabolic disorders.
authors.affiliation: Institut de recherche de l'Hôpital Montfort, 713 Montreal Rd, 1E113, Ottawa, ON, K1K 0T2, Canada.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Mauger
authors.affiliation: Institut de recherche de l'Hôpital Montfort, 713 Montreal Rd, 1E113, Ottawa, ON, K1K 0T2, Canada.|Faculty of Medicine, Biochemistry, Microbiology and Immunology Department, University of Ottawa, Ottawa, ON, K1H 8L1, Canada.
authors.forename: Lucien
authors.initials: L
authors.lastname: Nadeau
authors.affiliation: Institut de recherche de l'Hôpital Montfort, 713 Montreal Rd, 1E113, Ottawa, ON, K1K 0T2, Canada.|Faculty of Health Sciences, Interdisciplinary School of Health Sciences, University of Ottawa, Ottawa, ON, K1S 5N5, Canada.
authors.forename: Audrey
authors.initials: A
authors.lastname: Caron
authors.affiliation: Institut de recherche de l'Hôpital Montfort, 713 Montreal Rd, 1E113, Ottawa, ON, K1K 0T2, Canada.|Faculty of Health Sciences, School of Human Kinetics, University of Ottawa, Ottawa, ON, K1S 5N5, Canada.
authors.forename: Natalie Ann
authors.initials: NA
authors.lastname: Chapados
authors.affiliation: Institut de recherche de l'Hôpital Montfort, 713 Montreal Rd, 1E113, Ottawa, ON, K1K 0T2, Canada. celineaguer@montfort.on.ca.|Faculty of Medicine, Biochemistry, Microbiology and Immunology Department, University of Ottawa, Ottawa, ON, K1H 8L1, Canada. celineaguer@montfort.on.ca.
authors.forename: Céline
authors.initials: C
authors.lastname: Aguer
mesh_terms: D000818:Animals; D002460:Cell Line; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D006706:Homeostasis; D008928:Mitochondria / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010101:Oxygen Consumption; D010865:Pilot Projects; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats
chemical_list: D011078:Polychlorinated Biphenyls; D005947:Glucose; C023035:3,4,5,3',4'-pentachlorobiphenyl
keywords: Glucose metabolism; Oxidative phosphorylation; Oxidative stress; Persistent organic pollutants; Skeletal muscle