title: [Antifungal Activity of Luliconazole Nail Solution on in vitro and in vivo Onychomycosis Model].
abstract: We evaluated luliconazole nail solution, originally generated formulation, for the topical treatment of onychomycosis by two infection models. First, a suspension of Trichophyton mentagrophytes was dropped onto the ventral layer of human nail plate and these nails were set in Franz diffusion cells. After 9-day culture, luliconazole nail solutions (1, 3, and 5%) were applied to the dorsal surface of the nails once a day for 7 days. After application, fungal viability was assessed by measuring the ATP contents of the samples. The dose-dependent efficacy was confirmed, with 3% and 5% luliconazole nail solutions producing significantly lower ATP levels at 7-day treatment. When 3% and 5% luliconazole nail solutions were evaluated in a rabbit model of onychomycosis, both concentrations completely inhibited the recovery of fungi on culture after 4-week treatment. We therefore think these results indicate that 5% luliconazole nail solution is sufficiently potent for treatment of onychomycosis.
authors.affiliation: R&D Laboratories, POLA PHARMA INC.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shimamura
authors.forename: Nami
authors.initials: N
authors.lastname: Hasegawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kubota
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000287:Administration, Topical; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D025141:Drug Resistance, Fungal; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage* / Q000494:pharmacology*; D008297:Male; D014009:Onychomycosis / Q000188:drug therapy* / Q000382:microbiology*; D011817:Rabbits; D012996:Solutions; D016896:Treatment Outcome; D014249:Trichophyton / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; D012996:Solutions; D000255:Adenosine Triphosphate; C112528:luliconazole