title: Low level of FOXL1 indicates a worse prognosis for gastric cancer patients.
abstract: The aim of this study was to detect forkhead box L1 (FOXL1) expression in gastric cancer (GC) and to analyze its association with the prognosis of GC patients. Immunohistochemical staining, Western blotting, and quantitative reverse transcriptase polymerase chain reaction were performed to detect FOXL1 tissue expression in 109 GC patients. Clinicopathological characteristics and survival data were retrospectively analyzed to discover the clinical importance of FOXL1 expression. The chi-square test was used to analyze the relationship between FOXL1 expression and the clinicopathological characteristics. Survival curves were plotted by using the Kaplan-Meier method and compared using the log-rank test. Survival data were evaluated using univariate and multivariate Cox regression analyses. The expression of FOXL1 messenger RNA (mRNA) was significantly higher in adjacent normal samples than in the tumor tissues (P = 0.043). Clinicopathological analysis showed that FOXL1 expression was significantly correlated with depth of invasion (P = 0.017), lymph node metastasis (P = 0.019), and distant metastasis (P = 0.047). FOXL1-negative as opposed to the FOXL1-positive patients had lower 5-year overall survival (14.06 vs. 37.78 %, P < 0.001). Multivariate analysis suggested that FOXL1 expression might be an independent prognostic indicator (hazard ratio = 0.307, 95 % confidence interval, 0.187-0.505; P < 0.001) for GC patients. In conclusion, our findings provide evidence that FOXL1 might serve as a candidate tumor suppressor and a potential prognostic biomarker for GC.
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China.
authors.forename: Zhai
authors.initials: Z
authors.lastname: Ertao
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jianhui
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chuangqi
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Changjiang
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Sile
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China.
authors.forename: He
authors.initials: H
authors.lastname: Yulong
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China. Caisrteam@163.com.
authors.forename: Cai
authors.initials: C
authors.lastname: Shirong
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd road, Guangzhou, Guangdong, 510080, China. docwuhui@126.com.
authors.forename: Wu
authors.initials: W
authors.lastname: Hui
mesh_terms: D000230:Adenocarcinoma / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D018572:Disease-Free Survival; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000401:mortality / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C491001:FOXL1 protein, human; D051858:Forkhead Transcription Factors
keywords: FOXL1; Gastric cancer; Overall survival; Prognosis