title: miR-203 inhibits cell proliferation and promotes cisplatin induced cell death in tongue squamous cancer.
abstract: Oral squamous cell carcinoma (OSCC) is one of the most common types of the head and neck cancer. Chemo resistance of OSCC has been identified as a substantial therapeutic hurdle. In this study, we analyzed the role of miR-203 in the OSCC and its effects on cisplatin-induced cell death in an OSCC cell line, Tca8113. There was a significant decrease of miR-203 expression in OSCC samples, compared with the adjacent normal, non-cancerous tissue. After 3 days cisplatin treatment, the survived Tca8113 cells had a lower expression of miR-203 than that in the untreated control group. In contrast, PIK3CA showed an inverse expression in cancer and cisplatin survived Tca8113 cells. Transfection of Tca8113 cells with miR-203 mimics greatly reduced PIK3CA expression and Akt activation. Furthermore, miR-203 repressed PIK3CA expression through targeting the 3'UTR. Restoration of miR-203 not only suppressed cell proliferation, but also sensitized cells to cisplatin induced cell apoptosis. This effect was absent in cells that were simultaneously treated with PIK3CA RNAi. In summary, these findings suggest miR-203 plays an important role in cisplatin resistance in OSCC, and furthermore delivery of miR-203 analogs may serve as an adjuvant therapy for OSCC.
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510055, China.
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Stomatology, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, 510010, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kuang
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Wuhou District, Chengdu, 610041, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Wu
authors.affiliation: Patient-specific Orthopedic Technology Research Center in GuangDong Research Centre for Neural Engineering, 1068 Xueyuan Boulevard, University Town of Shenzhen, Xili, Nanshan, Shenzhen, 518055, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, The Fourth Military Medical University, Xi'an, Shanxi, 710032, China.
authors.forename: Qiaofeng
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Department of Orthodontics, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China. Electronic address: jasminenov@163.com.
authors.forename: Jiali
authors.initials: J
authors.lastname: Tan
mesh_terms: D020413:3' Untranslated Regions; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016923:Cell Death / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D058534:Class I Phosphatidylinositol 3-Kinases; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014059:Tongue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014062:Tongue Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D000970:Antineoplastic Agents; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human; D002945:Cisplatin
keywords: Cell death; Cell proliferation; PI3KCA; Tongue squamous-cancer; miR-203