title: Non-coding RNA LINC00473 mediates decidualization of human endometrial stromal cells in response to cAMP signaling.
abstract: Decidualization is an essential step in the establishment of pregnancy. However, the functional contributions of long intergenic noncoding RNAs (LincRNAs) to decidualization have not been explored. To explore the regulation and role of LincRNAs during human decidualization, human endometrial stromal cells (HESCs) are induced to undergo in vitro decidualization by treating with estradiol-17β, db-cAMP and medroxyprogesterone acetate. LINC00473 (LINC473) expression is highly induced in HESCs after decidual stimulus. We found that cAMP-PKA pathway regulates the expression of LINC473 through IL-11-mediated STAT3 phosphorylation. RNA interference-mediated down-regulation of LINC473 inhibits in vitro decidualization. These results suggested that LINC473 might be functionally required for human decidualization. This is the first report demonstrating the presence of LincRNA during human decidualization.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Xiao-Huan
authors.initials: XH
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Yue-Fang
authors.initials: YF
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Liang
authors.affiliation: Department of Biology, Shantou University, Shantou, China.
authors.forename: Zong-Min
authors.initials: ZM
authors.lastname: Fan
authors.affiliation: Department of Biology, Shantou University, Shantou, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Gu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Reproductive Medicine Center, Bailu Hospital, Xiamen, China.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Sha
authors.affiliation: Reproductive Medicine Center, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
authors.forename: Hong-Lu
authors.initials: HL
authors.lastname: Diao
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.|Department of Biology, Shantou University, Shantou, China.
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D003656:Decidua / Q000166:cytology*; D004958:Estradiol / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D017258:Medroxyprogesterone Acetate / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000378:metabolism*; D015398:Signal Transduction; D017154:Stromal Cells / Q000187:drug effects* / Q000502:physiology*
chemical_list: D062085:RNA, Long Noncoding; D004958:Estradiol; D017258:Medroxyprogesterone Acetate; D000242:Cyclic AMP