title: Development of pan-phlebovirus RT-PCR assay.
abstract: This study reports the pan-phlebovirus assay capable of detecting both sandfly/mosquito- and tick-borne phleboviruses. Sensitivity and specificity of the assay was verified using a panel of arboviruses. The RT-PCR assay is simple and sensitive, and thus well suited for screening of field samples.
authors.affiliation: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia; D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Klimentov
authors.affiliation: D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Butenko
authors.affiliation: D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Khutoretskaya
authors.affiliation: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia.
authors.forename: Elena Yu
authors.initials: EY
authors.lastname: Shustova
authors.affiliation: D.I. Ivanovsky Institute of Virology of N.F. Gamaleya Center of Epidemiology and Microbiology, Gamaleya Str. 16, Moscow 123098, Russia.
authors.forename: Victor F
authors.initials: VF
authors.lastname: Larichev
authors.affiliation: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Isaeva
authors.affiliation: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia; M.V. Lomonosov Moscow State University, Department of Biology, Leninskiye Gory Str. 1, Moscow 119991, Russia.
authors.forename: Galina G
authors.initials: GG
authors.lastname: Karganova
authors.affiliation: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Lukashev
authors.affiliation: Chumakov Institute of Poliomyelitis and Viral Encephalitides, Kievskoe shosse 27km., Moscow 142782, Russia. Electronic address: apgmyl@mail.ru.
authors.forename: Anatoly P
authors.initials: AP
authors.lastname: Gmyl
mesh_terms: D002044:Bunyaviridae Infections / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016856:Phlebovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
keywords: Phlebovirus; RT-PCR assay; Sandfly/mosquito-borne; Tick-borne