title: Effects of different cryoprotectants and freezing methods on post-thaw boar semen quality.
abstract: The current study aimed to investigate the effects of different concentrations of glycerol (0%, 1%, 2%, 3%, and 5%) and dimethylacetamide (DMA: 0%, 1%, 3%, and 5%) on post-sperm quality characteristics following semen freezing in dry ice (D) or liquid nitrogen (N). Semen was collected from Duroc boars and was allocated to 32 treatment groups for cryopreservation. Analysis of post-thaw semen quality and fertility after artificial insemination (AI) was used to examine the combinatorial effects of different treatments. The best scores for post-thaw sperm motility, sperm viability, and sperm acrosomal integrity were observed in semen frozen in: (a) dry ice in the presence of 5% glycerol and no DMA (16D-treatment); (b) dry ice in the presence of 3% glycerol and no DMA (9D-treatment); and (c) liquid nitrogen in the presence of 3% glycerol and 1% DMA (10N-treatment), with no significant difference observed among these three treatments. The farrowing rates after AI with post-thawed semen after 9D- and 10N-treatments were 33% and 50%, respectively. To summarize, the results of the present study indicated that the freezing extender containing 3% glycerol in combination with the straw-freezing method using dry ice produced the best post-thaw quality parameters of boar semen. Combinations of glycerol and DMA did not enhance the cryosurvival of boar spermatozoa.
authors.affiliation: Department of Veterinary Medicine, College of Agriculture, National Chiayi University, 580 Xinmin Road, Chiayi City, Taiwan.
authors.forename: Chung-Hsun
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Department of Veterinary Medicine, College of Agriculture, National Chiayi University, 580 Xinmin Road, Chiayi City, Taiwan.
authors.forename: Ting-Wen
authors.initials: TW
authors.lastname: Wu
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, Taiwan.
authors.forename: Feng-Pang
authors.initials: FP
authors.lastname: Cheng
authors.affiliation: Department of Veterinary Medicine, College of Agriculture, National Chiayi University, 580 Xinmin Road, Chiayi City, Taiwan.
authors.forename: Jiann-Hsiung
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, College of Agriculture, National Chiayi University, 580 Xinmin Road, Chiayi City, Taiwan; Animal Teaching Hospital, College of Agriculture, National Chiayi University, 580 Xinmin Road, Chiayi City, Taiwan. Electronic address: jtwu@mail.ncyu.edu.tw.
authors.forename: Jui-Te
authors.initials: JT
authors.lastname: Wu
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology; D005260:Female; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000187:drug effects*; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000502:physiology*
chemical_list: D000081:Acetamides; D003451:Cryoprotective Agents; C013959:dimethylacetamide; D005990:Glycerol
keywords: Boar; Cryopreservation; Dimethylacetamide; Glycerol; Semen