title: Pseudoroseicyclus aestuarii gen. nov., sp. nov., isolated from a tidal flat.
abstract: A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterial strain, designated DB-4T, was isolated from a tidal flat of the Yellow Sea, South Korea. Strain DB-4T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of approximately 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain DB-4T clustered with the type strain of Roseicyclus mahoneyensis, showing sequence similarity of 94.8 %. It exhibited sequence similarity values of 95.6-96.1 % to the type strains of Loktanella soesokkakensis, Loktanella hongkongensis, Loktanella variabilis, Loktanella aestuariicola and Loktanella cinnabarina. Strain DB-4T contained Q-10 as the predominant ubiquinone and C18:1ω7c and cyclo C19:0ω8c as the major fatty acids. The major polar lipids of strain DB-4T were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. The fatty acid and polar lipid profiles of strain DB-4T were distinguished from those of the type strains of R. mahoneyensis and some phylogenetically related Loktanella species. The DNA G+C content of strain DB-4T was 69.2 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain DB-4T is considered to represent a novel species of a new genus within the family Rhodobacteraceae, for which the name Pseudoroseicyclus aestuarii gen. nov., sp. nov. is proposed. The type strain ofPseudoroseicyclus aestuarii is DB-4T (=KCTC 52038T=CECT 9025T).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10