title: Amelioration of excision wounds by topical application of green synthesized, formulated silver and gold nanoparticles in albino Wistar rats.
abstract: Wound healing, a complex biological process, has attained a lot of attention as dermatologists are primarily interested in stimulated wound closure without formation of scar or a faint scar. The recent upsurgence of nanotechnology has provided novel therapeutic materials in the form of silver and gold nanoparticles which accelerate the wound healing process. The effect of formulated nanoparticles using Coleus forskohlii root extract (green synthesized) has been tried out for ameliorating full thickness excision wounds in albino Wistar male rats. The evaluation of in vivo activity of nanoparticles in wound healing was carried out on open wounds made by excision on the dorsal sides of albino Wistar rats under anesthesia, and the healing of the wounds was assessed. Histological aspects of the healing process were studied by a HE (Hematoxylin and Eosin) staining method to assess various degrees of re-epithelialization and the linear alignment of the granulation tissue whereas Van Gieson's histochemical staining was performed to observe collagen fibers. The healing action shown by the formulated nanoparticles was remarkable during the early stages of wound healing, which resulted in the substantial reduction of the whole healing period. Topical application of formulated gold nanoparticles was found to be more effective in suppressing inflammation and stimulating re-epithelialization compared to silver nanoparticles during the healing process. The results throw light on the amelioration of excision wounds using nanoparticles which could be a novel therapeutic way of improving wound healing in clinical practice. The mechanism of advanced healing action of both types of nanoparticles could be due to their antimicrobial, antioxidant and anti-inflammatory properties.
authors.affiliation: Chemistry Division, School of Advanced Sciences, VIT University, Vellore, 632 014, Tamilnadu, India.
authors.forename: Saraschandra
authors.initials: S
authors.lastname: Naraginti
authors.affiliation: Department of Bio-Technology, Sree Vidyanikethan Engineering College, Tirupathi, 517102, Andhra Pradesh, India.
authors.forename: P Lakshmi
authors.initials: PL
authors.lastname: Kumari
authors.affiliation: School of Bio-Sciences and Technology, VIT University, Vellore, 632 014, Tamilnadu, India.
authors.forename: Raunak Kumar
authors.initials: RK
authors.lastname: Das
authors.affiliation: Chemistry Division, School of Advanced Sciences, VIT University, Vellore, 632 014, Tamilnadu, India. Electronic address: svkmr7627@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Sivakumar
authors.affiliation: Center for Material Characterization, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, Pune, 411008, India.
authors.forename: Sagar Hindurao
authors.initials: SH
authors.lastname: Patil
authors.affiliation: Chemistry Technology Centre of Catalonia, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Vaibhav Vilas
authors.initials: VV
authors.lastname: Andhalkar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D031346:Plectranthus / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D062326:Re-Epithelialization / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D012867:Skin / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D012834:Silver; D006046:Gold
keywords: Collagen; Gold; Green synthesis; Nanoparticles; Wound healing