title: Derivation of Schwann cell precursors from neural crest cells resident in bone marrow for cell therapy to improve peripheral nerve regeneration.
abstract: We have previously successfully enriched post-migratory neural crest cells (NCCs) from postnatal rat bone marrow (BM). These BM-NCCs possess glial and neuronal differentiating potential. Based on the neural crest origin of Schwann cells (SCs), in this study, we aimed at using a straightforward protocol to derive Schwann cell precursors (SCPs) from BM-NCCs. Several clonal subpopulations were isolated from BM-NCCs, displaying long-term proliferative capacity and maintaining the NCC identity. The BM-NCC clones could be induced to differentiate into SCs. In particular, clone N1 gave rise to a large and pure population of SCs. Clone N1-derived SCs demonstrated the myelinating capacity in their co-culture with primary dorsal root ganglion (DRG) neurons. The decreased expression of NCC-markers and increased expression of SC-markers were related to the differentiation state of clone N1-derived SCs. To investigate the repair-promoting effects of clone N1 on injured peripheral neurons in vitro and in vivo, on one hand, the oxygen glucose deprivation-injured DRG neurons were treated with clone N1-conditioned medium, improving the cell survival and axon growth of neurons; on the other hand, clone N1 or clone N1-derived SCs were respectively implanted to the crush sciatic nerve of rats, and clone N1 yielded the better outcome of nerve regeneration and function restoration than clone N1-derived SCs. Taken together, all the results collectively showed that clone N1 could be identified as SCPs, which might hold promise for cell therapy to improve peripheral nerve regeneration.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, JS 226001, China; The Department of Pathophysiology, Medical School, Nantong University, Nantong, JS 226001, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shi
authors.affiliation: Hospital Affiliated to Nantong University, Nantong, JS 226001, China.
authors.forename: Yanpei
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, JS 226001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qiang
authors.affiliation: The Department of Pathophysiology, Medical School, Nantong University, Nantong, JS 226001, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, JS 226001, China.
authors.forename: Shibo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, JS 226001, China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Gao
authors.affiliation: The Department of Pathophysiology, Medical School, Nantong University, Nantong, JS 226001, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: The Department of Pathophysiology, Medical School, Nantong University, Nantong, JS 226001, China.
authors.forename: Ximeng
authors.initials: X
authors.lastname: Ji
authors.affiliation: The Department of Pathophysiology, Medical School, Nantong University, Nantong, JS 226001, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tian
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, JS 226001, China. Electronic address: nervegu@ntu.edu.cn.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration and Co-innovation Center of Neuroregeneration, Nantong University, Nantong, JS 226001, China. Electronic address: dingfei@ntu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000293:injuries / Q000502:physiology*; D009416:Nerve Regeneration; D009432:Neural Crest / Q000166:cytology*; D017208:Rats, Wistar; D012583:Schwann Cells / Q000166:cytology / Q000637:transplantation*; D012584:Sciatic Nerve / Q000293:injuries* / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned
keywords: BM-NCC clones; Bone marrow; Neural crest cells; Peripheral nerve regeneration; Schwann cell precursors