title: A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk.
abstract: To date, there are few reports regarding the development of RNA aptamer-based biosensors for the detection of small molecules. The possible reason is attributed to the weak nuclease resistance of RNA in biological environments. In this study, we have developed an RNA aptamer-based gold nanoparticle (AuNP) sensor for fluorometric detection of Neomycin B in milk. This aptasensor depends on the self-assembly of the RNA aptamer/Neomycin B complex and fluorescence quenching by AuNPs. This biosensor exhibited a low detection limit of 0.01 μM, with a linear dynamic range from 0.1 to 10 μM in milk, and a good selectivity toward Neomycin B. Specifically, because of the shorter RNA fragments and the nuclease inhibition ability of the RNA-modified AuNPs, the RNA sequences remained stable during the experiments. This work will serve as an example for the development of novel biosensors based on RNA aptamers. Graphical Abstract An RNA aptamer-based nanoparticle sensor, developed for the detection of Neomycin B in milk, shows high binding affinity and selectivity. This aptasensor depends on the self-assembly of the aptamer/ligand complex and fluorescence quenching by gold nanoparticles (AuNPs). Because of the shorter RNA fragments and the nuclease inhibition ability of RNA-modified AuNPs, RNA sequences remain stable during the detection.
authors.affiliation: The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, No. 1017 Dongmen North Road, Shenzhen, 518020, China.|School of Medicine, Jinan University, Guangzhou, 510632, China.|Shenzhen Institute of Geriatrics, Shenzhen, 518020, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Ling
authors.affiliation: Institute of Biomedical Engineering, Key Laboratory of Biomedical Material of Tianjin, Chinese Academy of Medical Sciences & Peking Union Medical College, 236 Baidi Road Nankai District, Tianjin, Tianjin, 300192, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Nuclear Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, 227 South Chongqing Road, Shanghai, 200092, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, No. 1017 Dongmen North Road, Shenzhen, 518020, China.|School of Medicine, Jinan University, Guangzhou, 510632, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, No. 1017 Dongmen North Road, Shenzhen, 518020, China.|School of Medicine, Jinan University, Guangzhou, 510632, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.affiliation: The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, No. 1017 Dongmen North Road, Shenzhen, 518020, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Qiu
authors.affiliation: The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, No. 1017 Dongmen North Road, Shenzhen, 518020, China. frli62@163.com.|School of Medicine, Jinan University, Guangzhou, 510632, China. frli62@163.com.
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Li
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D005601:Framycetin / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles; D008892:Milk / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D052157:Aptamers, Nucleotide; D005601:Framycetin; D006046:Gold
keywords: Aptasensor; Gold nanoparticle; Neomycin B; RNA aptamer; Self-assembly