title: Relative bioavailability of copper in tribasic copper chloride to copper in copper sulfate for laying hens based on egg yolk and feather copper concentrations.
abstract: This experiment was conducted to determine the relative bioavailability (RBV) of Cu in tribasic copper chloride (TBCC) to Cu in copper sulfate (monohydrate form; CuSO4·H2O) for layer diets based on egg yolk and feather Cu concentrations. A total of 252, 72-wk-old Hy-Line Brown laying hens were allotted to 1 of 7 treatments with 6 replicates consisting of 6 hens per replicate in a completely randomized design. Hens were fed corn-soybean meal-based basal diets supplemented with 0 (basal), 100, 200, or 300 mg/kg Cu from CuSO4 or TBCC for 4 wk. Results indicated that egg production, egg weight, and egg mass were not affected by dietary treatments. However, increasing inclusion levels of Cu in diets from CuSO4 decreased (P < 0.05) feed conversion ratio (FCR), whereas increasing inclusion levels of Cu in diets from TBCC did not affect FCR, indicating significant interaction (P < 0.05). Increasing inclusion levels of Cu from TBCC or CuSO4 increased (P < 0.05) Cu concentrations of egg yolk and feathers. Feather Cu concentrations were greater (P < 0.01) for hens fed diets containing CuSO4 than for hens fed diets containing TBCC. The values for the RBV of Cu in TBCC to Cu in CuSO4 based on log10 transformed egg yolk and feather Cu concentrations were 107.4% and 69.5%, respectively. These values for the RBV of Cu in TBCC did not differ from Cu in CuSO4 (100%). The RBV measured in egg yolk did not differ from the RBV measured in feather. In conclusion, the RBV of Cu in TBCC to Cu in CuSO4 can be determined using Cu concentrations of egg yolk and feathers although the values depend largely on target tissues of laying hens. For a practical application, however, the RBV value of Cu in TBCC to Cu in CuSO4 could be 88.5% when the RBV values determined using egg yolk and feather Cu concentrations were averaged.
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea.
authors.forename: J E
authors.initials: JE
authors.lastname: Shin
authors.affiliation: Department of Animal Science and Technology, Chung-Ang University, Anseong 456-756, Republic of Korea. Electronic address: dongyong@cau.ac.kr.
authors.forename: D Y
authors.initials: DY
authors.lastname: Kil
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001682:Biological Availability; D002645:Chickens / Q000378:metabolism*; D002712:Chlorides / Q000032:analysis / Q000493:pharmacokinetics*; D003300:Copper / Q000032:analysis / Q000493:pharmacokinetics*; D019327:Copper Sulfate / Q000032:analysis / Q000493:pharmacokinetics*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004530:Egg Yolk / Q000737:chemistry; D005241:Feathers / Q000737:chemistry; D005260:Female; D011897:Random Allocation
chemical_list: D002712:Chlorides; C111994:atacamite; D003300:Copper; D019327:Copper Sulfate
keywords: copper; egg yolk; feather; laying hen; relative bioavailability