title: MS/MS Digital Readout: Analysis of Binary Information Encoded in the Monomer Sequences of Poly(triazole amide)s.
abstract: Tandem mass spectrometry was evaluated as a reliable sequencing methodology to read codes encrypted in monodisperse sequence-coded oligo(triazole amide)s. The studied oligomers were composed of monomers containing a triazole ring, a short ethylene oxide segment, and an amide group as well as a short alkyl chain (propyl or isobutyl) which defined the 0/1 molecular binary code. Using electrospray ionization, oligo(triazole amide)s were best ionized as protonated molecules and were observed to adopt a single charge state, suggesting that adducted protons were located on every other monomer unit. Upon collisional activation, cleavages of the amide bond and of one ether bond were observed to proceed in each monomer, yielding two sets of complementary product ions. Distribution of protons over the precursor structure was found to remain unchanged upon activation, allowing charge state to be anticipated for product ions in the four series and hence facilitating their assignment for a straightforward characterization of any encoded oligo(triazole amide)s.
authors.affiliation: Aix-Marseille Université -CNRS, UMR 7273, Institute of Radical Chemistry, 13397 Marseille Cedex 20, France.
authors.forename: Jean-Arthur
authors.initials: JA
authors.lastname: Amalian
authors.affiliation: Precision Macromolecular Chemistry, Institut Charles Sadron , UPR22-CNRS, BP84047, 23 Rue du Loess, 67034 Strasbourg Cedex 2, France.
authors.forename: Thanh Tam
authors.initials: TT
authors.lastname: Trinh
authors.affiliation: Precision Macromolecular Chemistry, Institut Charles Sadron , UPR22-CNRS, BP84047, 23 Rue du Loess, 67034 Strasbourg Cedex 2, France.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Lutz
authors.affiliation: Aix-Marseille Université -CNRS, UMR 7273, Institute of Radical Chemistry, 13397 Marseille Cedex 20, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Charles