title: Cadherin-11 localizes to focal adhesions and promotes cell-substrate adhesion.
abstract: Cadherin receptors have a well-established role in cell-cell adhesion, cell polarization and differentiation. However, some cadherins also promote cell and tissue movement during embryonic development and tumour progression. In particular, cadherin-11 is upregulated during tumour and inflammatory cell invasion, but the mechanisms underlying cadherin-11 stimulated cell migration are still incompletely understood. Here, we show that cadherin-11 localizes to focal adhesions and promotes adhesion to fibronectin in Xenopus neural crest, a highly migratory embryonic cell population. Transfected cadherin-11 also localizes to focal adhesions in different mammalian cell lines, while endogenous cadherin-11 shows focal adhesion localization in primary human fibroblasts. In focal adhesions, cadherin-11 co-localizes with β1-integrin and paxillin and physically interacts with the fibronectin-binding proteoglycan syndecan-4. Adhesion to fibronectin mediated by cadherin-11/syndecan-4 complexes requires both the extracellular domain of syndecan-4, and the transmembrane and cytoplasmic domains of cadherin-11. These results reveal an unexpected role of a classical cadherin in cell-matrix adhesion during cell migration.
authors.affiliation: Zoological Institute, Cell and Developmental Biology, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131 Karlsruhe, Germany.
authors.forename: Rahul P
authors.initials: RP
authors.lastname: Langhe
authors.affiliation: Center for Functional Nanostructures, Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1a, 76131 Karlsruhe, Germany.
authors.forename: Tetyana
authors.initials: T
authors.lastname: Gudzenko
authors.affiliation: Zoological Institute, Cell and Neurobiology Biology, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Strasse 9, 76131 Karlsruhe, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Bachmann
authors.affiliation: Zoological Institute, Cell and Developmental Biology, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131 Karlsruhe, Germany.
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Becker
authors.affiliation: Center for Functional Nanostructures, Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1a, 76131 Karlsruhe, Germany.
authors.forename: Carina
authors.initials: C
authors.lastname: Gonnermann
authors.affiliation: Zoological Institute, Cell and Developmental Biology, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131 Karlsruhe, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Winter
authors.affiliation: Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Genevieve
authors.initials: G
authors.lastname: Abbruzzese
authors.affiliation: Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003, USA.
authors.forename: Dominique
authors.initials: D
authors.lastname: Alfandari
authors.affiliation: Zoological Institute, Cell and Developmental Biology, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131 Karlsruhe, Germany.|Laboratory for Applications of Synchrotron Radiation, Karlsruhe Institute of Technology (KIT), Engesser Straße 15, 76131 Karlsruhe, Germany.
authors.forename: Marie-Claire
authors.initials: MC
authors.lastname: Kratzer
authors.affiliation: Center for Functional Nanostructures, Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1a, 76131 Karlsruhe, Germany.
authors.forename: Clemens M
authors.initials: CM
authors.lastname: Franz
authors.affiliation: Zoological Institute, Cell and Developmental Biology, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131 Karlsruhe, Germany.|Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Jubin
authors.initials: J
authors.lastname: Kashef
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D002477:Cells / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D022001:Focal Adhesions / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009432:Neural Crest / Q000254:growth & development / Q000378:metabolism; D021381:Protein Transport; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D005353:Fibronectins; C086960:osteoblast cadherin