title: Chromatographic separation of germanium and arsenic for the production of high purity (77)As.
abstract: A simple column chromatographic method was developed to isolate (77)As (94±6% (EtOH/HCl); 74±11 (MeOH)) from germanium for potential use in radioimmunotherapy. The separation of arsenic from germanium was based on their relative affinities for different chromatographic materials in aqueous and organic environments. Using an organic or mixed mobile phase, germanium was selectively retained on a silica gel column as germanate, while arsenic was eluted from the column as arsenate. Subsequently, enriched (76)Ge (98±2) was recovered for reuse by elution with aqueous solution (neutral to basic). Greater than 98% radiolabeling yield of a (77)As-trithiol was observed from methanol separated [(77)As]arsenate [17].
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO 65211, United States.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Gott
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO 65211, United States.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: DeGraffenreid
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO 65211, United States.
authors.forename: Yutian
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO 65211, United States.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Phipps
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO 65211, United States.
authors.forename: Donald E
authors.initials: DE
authors.lastname: Wycoff
authors.affiliation: University of Missouri Research Reactor Center, Columbia, MO 65211, United States.
authors.forename: Mary F
authors.initials: MF
authors.lastname: Embree
authors.affiliation: University of Missouri Research Reactor Center, Columbia, MO 65211, United States.
authors.forename: Cathy S
authors.initials: CS
authors.lastname: Cutler
authors.affiliation: University of Missouri Research Reactor Center, Columbia, MO 65211, United States.
authors.forename: Alan R
authors.initials: AR
authors.lastname: Ketring
authors.affiliation: Department of Chemistry, University of Missouri, Columbia, MO 65211, United States. Electronic address: jurissons@missouri.edu.
authors.forename: Silvia S
authors.initials: SS
authors.lastname: Jurisson
mesh_terms: D001151:Arsenic / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D005857:Germanium / Q000302:isolation & purification*; D000432:Methanol; D011868:Radioisotopes / Q000302:isolation & purification; D058428:Silica Gel; D012997:Solvents; D014867:Water
chemical_list: D011868:Radioisotopes; D012997:Solvents; D005857:Germanium; D014867:Water; D058428:Silica Gel; D001151:Arsenic; D000432:Methanol
keywords: Column chromatography; Ge/As distribution coefficient; Ge/As separation; High specific activity arsenic-77