title: Profiling of acidic (amino and phenolic acids) and phenylpropanoids production in response to methyl jasmonate-induced oxidative stress in Scrophularia striata suspension cells.
abstract: A metabolic profiling including calculation of energy cost of amino acids biosynthesis in cultured cells of Scrophularia striata showed that methyl jasmonate-inducible oxidative stress elicited secondary metabolites formation derived from phenylalanine and tyrosine and increased energy cost for these amino acids biosynthesis. Understanding of the metabolic pathways in cell culture of Scrophularia striata, an aromatic plant species, facilitates means of production of pharmaceutical metabolites under oxidative stress. In this study, we evaluated the effects of MeJA on the S. striata metabolic pathway and the responses to oxidative stress. Exposure to methyl jasmonate (MeJA) affects plant growth, effectively induces production of reactive oxygen species (ROS) and inserts oxidative stress at the cellular level which results in alteration of primary metabolites and production of phenylepropanoid compounds. Cells treated with MeJA indicated increase in the activities of three antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPx) as well as intracellular H2O2 and MDA contents compared with mock-treated cells. High performance liquid chromatography (HPLC)-based metabolome analysis revealed dynamic metabolic changes in oxidatively stressed S. striata cells, e.g., general phenylpropanoid pathway, phenylethanoid-glycosides, lignans, and increased energy cost of biosynthesis and accumulation of amino acids. Furthermore, principal component analysis (PCA)-derived score plots demonstrated that MeJA affects cellular metabolism in S. striata cells and significantly alters metabolite composition under MeJA-inducible oxidative stress. These observations suggest that MeJA-elicited cell suspension cultures of S. striata balanced the production of primary and secondary metabolites in coordination with ROS-scavenging system.
authors.affiliation: Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Sadeghnezhad
authors.affiliation: Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. msharifi@modares.ac.ir.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sharifi
authors.affiliation: Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Zare-Maivan
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000596:Amino Acids / Q000096:biosynthesis*; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics; D018384:Oxidative Stress / Q000187:drug effects*; D054883:Oxylipins / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D020005:Propanols / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D032266:Scrophularia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000085:Acetates; D000596:Amino Acids; D003517:Cyclopentanes; D062385:Hydroxybenzoates; D054883:Oxylipins; D010937:Plant Growth Regulators; D020005:Propanols; D017382:Reactive Oxygen Species; C439395:1-phenylpropanol; D008315:Malondialdehyde; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; C121048:guaiacol peroxidase; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; C017616:phenolic acid
keywords: Antioxidant enzymes; Cell suspension; Energy cost; Metabolome; Methyl jasmonate; Scrophularia striata