title: Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma.
abstract: The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.
authors.affiliation: Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal. ana_mamede@hotmail.com.|CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal. ana_mamede@hotmail.com.|CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, Portugal. ana_mamede@hotmail.com.|CNC.IBILI, University of Coimbra, Coimbra, Portugal. ana_mamede@hotmail.com.
authors.forename: A C
authors.initials: AC
authors.lastname: Mamede
authors.affiliation: Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Guerra
authors.affiliation: Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.|CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.|CNC.IBILI, University of Coimbra, Coimbra, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Laranjo
authors.affiliation: Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.
authors.forename: K
authors.initials: K
authors.lastname: Santos
authors.affiliation: Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.|CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.|CNC.IBILI, University of Coimbra, Coimbra, Portugal.|Obstetrics Service, Coimbra Hospital and University Centre, Coimbra, Portugal.
authors.forename: M J
authors.initials: MJ
authors.lastname: Carvalho
authors.affiliation: Blood and Transplantation Center of Coimbra, Portuguese Institute of the Blood and Transplantation, Coimbra, Portugal.
authors.forename: T
authors.initials: T
authors.lastname: Carvalheiro
authors.affiliation: Obstetrics Service, Coimbra Hospital and University Centre, Coimbra, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Moura
authors.affiliation: Cytometry Operational Management Unit, Clinical Pathology Department, Coimbra Hospital and University Centre, Coimbra, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Paiva
authors.affiliation: Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.|CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.|CNC.IBILI, University of Coimbra, Coimbra, Portugal.
authors.forename: A M
authors.initials: AM
authors.lastname: Abrantes
authors.affiliation: CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal.
authors.forename: C J
authors.initials: CJ
authors.lastname: Maia
authors.affiliation: Biophysics Unit, Faculty of Medicine, University of Coimbra, Azinhaga de Santa Comba - Celas, 3000-548, Coimbra, Portugal.|CIMAGO, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.|CNC.IBILI, University of Coimbra, Coimbra, Portugal.
authors.forename: M F
authors.initials: MF
authors.lastname: Botelho
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000378:metabolism*; D000650:Amnion / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D000970:Antineoplastic Agents; C493956:CDKN1A protein, human; D018797:Cell Cycle Proteins; D050759:Cyclin-Dependent Kinase Inhibitor p21; D008565:Membrane Proteins; D016159:Tumor Suppressor Protein p53; D051176:beta Catenin; D004247:DNA
keywords: Cell cycle; Hepatocellular carcinoma; Human amniotic membrane; Oxidative stress; Protein extracts; hAMPE