title: Presence of blaPER-1 and blaVEB-1 beta-lactamase genes among isolates of Pseudomonas aeruginosa from South West of Iran.
abstract: Pseudomonas aeruginosa isolates have acquired resistance to antibiotics such as novel beta-lactams. The aim of this study was to investigate the blaPER-1, blaVEB-1, and blaPSE-1 genes among isolates of P. aeruginosa among intensive care unit (ICU) patients. Sixty-five isolates were collected. The antibiotic susceptibility testing and combined disk tests were performed to detect the isolates producing extended spectrum beta-lactamases (ESBLs) among ceftazidime-resistant isolates. Polymerase chain reaction (PCR) amplification of blaPER-1, blaVEB-1, and blaPSE-1 genes was conducted. Ten (15.3%) isolates were ESBL-positive, of which 40% (n=4) belonged to males and 60% (n=6) were collected from females. Moreover, two and one isolates harbored blaPER-1 and blaVEB-1 genes, respectively.
authors.affiliation: Department of Microbiology, School of Paramedical Sciences, Ilam University of Medical Sciences, Ilam, Iran. Electronic address: Elhamdavodian@yahoo.com.
authors.forename: Elham
authors.initials: E
authors.lastname: Davodian
authors.affiliation: Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Nourkhoda
authors.initials: N
authors.lastname: Sadeghifard
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Abdolmajid
authors.initials: A
authors.lastname: Ghasemian
authors.affiliation: Research Center of Pediatric Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Samileh
authors.initials: S
authors.lastname: Noorbakhsh
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007492:Iran; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C108863:beta-lactamase PER-1; D001618:beta-Lactamases; C000609545:beta-lactamase VEB-1, Pseudomonas aeruginosa
keywords: Beta-lactamase; ESBLs; ICU patients; Pseudomonas aeruginosa