title: Effects of Different Limb Remote Ischaemic Preconditioning on Ischaemia Reperfusion Injury in an Acute Left Anterior Descending Artery Occlusion Rat Model.
abstract: BACKGROUND
  The aim is to compare effects of three different protocols of limb remote ischaemic preconditioning (LRIP) on ischaemia reperfusion injury in an acute left anterior descending artery (LAD) occlusion model rat.
  
  
  METHODS
  Forty adult male Wistar rats were randomly assigned into four groups: group A, control; group B, LRIP in bilateral upper-limb (BUL) IP; group C, LRIP in bilateral lower-limb (BLL) IP; group D, LRIP in bilateral upper and lower limbs (ULL) IP. The 60min ligation and 180min reperfusion in LAD were applied to all rats. Limb remote ischaemic preconditioning was performed using 5min occlusion and 15min reperfusion (six cycles). Heart rate, blood pressure and electrogastrography (EGG) were recorded. Creatine kinase isoenzyme (CK-MB) level and infarct size were measured.
  
  
  RESULTS
  Limb remote ischaemic preconditioning did not significantly affect heart rate, systolic blood pressure and arrhythmia score. However, LRIP significantly increased DBP value and decreased CK-MB levels and infarct size in group B, C, and D. Moreover, LRIP in ULL had a significantly better effect on reducing infarct size than LRIP in BUL and BLL.
  
  
  CONCLUSIONS
  Limb remote ischaemic preconditioning at limbs could significantly reduce reperfusion injury in the heart. Moreover, LRIP in ULL indicated a better effect in reducing infarct size than LRIP in BUL and BLL.
authors.affiliation: Department of Cardiology, Shanghai Pudong New District Zhoupu Hospital, The People's Republic of China.
authors.forename: Yingbiao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, The People's Republic of China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Hong
authors.affiliation: Department of Cardiology, Harbin Chest Hospital, Harbin, The People's Republic of China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.affiliation: Department of Radiotherapy, The Second Affiliated Hospital, Shanxi Medical University, Shanxi, The People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wei
authors.affiliation: Department of Endocrinology, Qiqihar First Hospital, Qiqihar, The People's Republic of China.
authors.forename: Xuhong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Mudanjiang First People's Hospital, Mudanjiang, The People's Republic of China.
authors.forename: Keji
authors.initials: K
authors.lastname: Liang
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, Harbin Medical University, Harbin, The People's Republic of China. Electronic address: junjiekouou@163.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Kou
mesh_terms: D000818:Animals; D001794:Blood Pressure; D052279:Creatine Kinase, MB Form / Q000097:blood; D006339:Heart Rate; D006614:Hindlimb / Q000098:blood supply / Q000503:physiopathology; D019194:Ischemic Preconditioning; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood* / Q000503:physiopathology*
chemical_list: D052279:Creatine Kinase, MB Form
keywords: Infarct size; Limbs preconditioning; Remote preconditioning; Reperfusion injury