title: Dark Field Microscopic Sensitive Detection of Amyloid Fibrils Using Gold Nanoparticles Modified with Antibody.
abstract: Dark field microscopy (DFM) was employed to detect amyloid β (Aβ) fibrils-induced gold nanoparticle (AuNP) aggregation at the single-particle level, with a detection limit of 40 pM fibrils. The sensitivity of this method is higher than that of the current fibril-specific detection method using probe dye, such as thioflavin T, for which sub-μM level of fibrils are necessary. This study further proved the potential application of DFM in the analytical methods based on AuNP aggregation.
authors.affiliation: Department of Advanced Materials Science, School of Frontier Sciences, The University of Tokyo.
authors.forename: Tong
authors.initials: T
authors.lastname: Bu
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Zako
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000276:immunology; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008853:Microscopy; D010446:Peptide Fragments / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D016229:Amyloid beta-Peptides; D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D006046:Gold