title: Bioinformatic and Expression Analysis of Ventricular Septal Defect-associated Long Non-coding RNA TUC40-.
abstract: OBJECTIVE
  To explore the potential role of TUC40- in human and mouse embryonic heart development.
  
  
  METHODS
  Bioinformatics databases including NCBI,UCSC,and Uniprot and software including Clustal,DNAMAN,and MEGA 6 were used to collect information of TUC40- and uc.40-. The expression profile at key time points of heart development was investigated by strand-specific quantitative real time polymerase chain reaction.
  
  
  RESULTS
  Uc.40- was conservative in sequence, genomic location, and transcription factor binding sites across human and mouse. Pbx1/TUC40- showed negative trend during embryonic mouse heart maturation.
  
  
  CONCLUSIONS
  Various levels of conservation of uc.40- suggests similar functions of TUC40- in these two species. TUC40- may play its roles in human and mouse embryonic heart development by regulating Pbx1.
authors.affiliation: Department of Pediatrics,Zhongda Hospital,Southeast University,Nanjing 210009,China.
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Li
authors.affiliation: Department of Pediatrics,Zhongda Hospital,Southeast University,Nanjing 210009,China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Pediatrics,Nanjing Maternity and Child Health Care Hospital,Nanjing Medical University,Nanjing 210004,China.
authors.forename: Zhang-bin
authors.initials: ZB
authors.lastname: Yu
authors.affiliation: Department of Pediatrics,Nanjing Maternity and Child Health Care Hospital,Nanjing Medical University,Nanjing 210004,China.
authors.forename: Shu-ping
authors.initials: SP
authors.lastname: Han
authors.affiliation: Department of Pediatrics,Nanjing Maternity and Child Health Care Hospital,Nanjing Medical University,Nanjing 210004,China.
authors.forename: Xue-hua
authors.initials: XH
authors.lastname: Liu
mesh_terms: D000818:Animals; D019295:Computational Biology; D006345:Heart Septal Defects, Ventricular; D006801:Humans; D051379:Mice; D062085:RNA, Long Noncoding; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D062085:RNA, Long Noncoding