title: False ALK Rearrangement Signals in Inflammatory Cells: A Pitfall in the Interpretation of ALK Fluorescence In Situ Hybridization in Non-Small Cell Lung Cancer.
abstract: Searching for ALK rearrangements has now become mandatory for the treatment of patients with advanced non-small cell lung cancer with anti-ALK-targeted therapy. The fluorescence in situ hybridization test is considered the "gold standard" to diagnose ALK-rearranged tumors. Nevertheless, some technical pitfalls may cause false-positive signals mimicking ALK rearrangements. In this technical article, we point out the importance of taking into account both histopathologic and ALK immunohistochemical features to interpret ALK fluorescence in situ hybridization analyses in inflammatory and necrotic tumors. This confrontation is crucial to avoid misdiagnosis and inappropriate therapeutic management.
authors.affiliation: *Inserm, U1078 †Department of Pathology, Brest University Hospital ‡European University of Brittany §EA4685, Brest University ∥Department of Cytogenetics, Brest University Hospital, Brest, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Uguen
authors.forename: Matthieu
authors.initials: M
authors.lastname: Talagas
authors.forename: Sophie
authors.initials: S
authors.lastname: Andrieu-Key
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quintin-Roué
authors.forename: Pascale
authors.initials: P
authors.lastname: Marcorelles
authors.forename: Marc
authors.initials: M
authors.lastname: De Braekeleer
mesh_terms: D000368:Aged; D000077548:Anaplastic Lymphoma Kinase; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*
chemical_list: C000626173:ALK protein, human; D000077548:Anaplastic Lymphoma Kinase; D020794:Receptor Protein-Tyrosine Kinases