title: Inner nuclear membrane localization of epidermal growth factor receptor (EGFR) in spontaneous canine model of invasive micropapillary carcinoma of the mammary gland.
abstract: The epidermal growth factor receptor (EGFR) has been described in the nucleus of primary tumors. Accumulation of EGFR at the nucleus is linked to DNA synthesis and cell proliferation, but the pathological significance of nuclear EGFR is not completely understood. The aim of this study was to investigate the nuclear localization of EGFR in invasive micropapillary carcinoma (IMPC) that is an aggressive neoplasm of canine mammary gland. Confocal immunofluorescence of formalin and paraffin-embedded tissue was used to access the subcellular localization of EGFR. Our results demonstrated that EGFR co-localizes with the inner nuclear envelope marker, Lamin B1 in IMPC. Furthermore, EGFR was not localized within the nucleus or at the inner nuclear envelope membrane in mammary carcinoma in mixed tumor (CMT) that is associated with a better prognosis than other malignant histological types. This finding could be useful as a predictive biomarker of therapeutic response for IMPC.
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil. Electronic address: michele.ip3@gmail.com.
authors.forename: Michele A
authors.initials: MA
authors.lastname: Rodrigues
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
authors.forename: Conrado O
authors.initials: CO
authors.lastname: Gamba
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
authors.forename: Jerusa Araújo Quintão Arantes
authors.initials: JA
authors.lastname: Faria
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
authors.forename: Ênio
authors.initials: Ê
authors.lastname: Ferreira
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
authors.forename: Alfredo M
authors.initials: AM
authors.lastname: Goes
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
authors.forename: Dawidson A
authors.initials: DA
authors.lastname: Gomes
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte CEP: 31270-901, MG, Brazil.
authors.forename: Geovanni D
authors.initials: GD
authors.lastname: Cassali
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002291:Carcinoma, Papillary / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D015674:Mammary Neoplasms, Animal / Q000378:metabolism* / Q000473:pathology; D018613:Microscopy, Confocal; D009685:Nuclear Envelope / Q000378:metabolism; D011379:Prognosis
chemical_list: D066246:ErbB Receptors
keywords: Confocal microscopy; Invasive micropapillary carcinoma; Nuclear EGFR