title: Biological characterization of a new silicon based coating developed for dental implants.
abstract: Taking into account the influence of Si in osteoblast cell proliferation, a series of sol-gel derived silicon based coating was prepared by controlling the process parameters and varying the different Si-alkoxide precursors molar rate in order to obtain materials able to release Si compounds. For this purpose, methyltrimethoxysilane (MTMOS) and tetraethyl orthosilicate (TEOS) were hydrolysed together and the sol obtained was used to dip-coat the different substrates. The silicon release ability of the coatings was tested finding that it was dependent on the TEOS precursor content, reaching a Si amount value around ninefolds higher for coatings with TEOS than for the pure MTMOS material. To test the effect of this released Si, the in vitro performance of developed coatings was tested with human adipose mesenchymal stem cells finding a significantly higher proliferation and mineralization on the coating with the higher TEOS content. For in vivo evaluation of the biocompatibility, coated implants were placed in the tibia of the rabbit and a histological analysis was performed. The evaluation of parameters such as the bone marrow state, the presence of giant cells and the fibrous capsule proved the biocompatibility of the developed coatings. Furthermore, coated implants seemed to produce a qualitatively higher osteoblastic activity and a higher number of bone spicules than the control (uncoated commercial SLA titanium dental implant).
authors.affiliation: Polymer Science and Technology Department, University of the Basque Country (UPV/EHU), P Manuel de Lardizabal, 3, 20018, San Sebastián, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Ibáñez
authors.affiliation: Polymer Science and Technology Department, University of the Basque Country (UPV/EHU), P Manuel de Lardizabal, 3, 20018, San Sebastián, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Juan-Díaz
authors.affiliation: Industrial Systems Engineering and Design Department, Jaime I University (UJI), 12071, Castellón de la Plana, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Lara-Saez
authors.affiliation: Ilerimplant SL, 25191, Lleida, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Coso
authors.affiliation: Ilerimplant SL, 25191, Lleida, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Franco
authors.affiliation: Polymer Science and Technology Department, University of the Basque Country (UPV/EHU), P Manuel de Lardizabal, 3, 20018, San Sebastián, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Gurruchaga
authors.affiliation: Industrial Systems Engineering and Design Department, Jaime I University (UJI), 12071, Castellón de la Plana, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Suay Antón
authors.affiliation: Polymer Science and Technology Department, University of the Basque Country (UPV/EHU), P Manuel de Lardizabal, 3, 20018, San Sebastián, Spain. isabel.goni@ehu.eus.
authors.forename: Isabel
authors.initials: I
authors.lastname: Goñi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001846:Bone Development; D001853:Bone Marrow; D015921:Dental Implants; D008422:Materials Testing; D011817:Rabbits; D012825:Silicon / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D012825:Silicon