title: Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2.
abstract: SR proteins are a class of RNA-binding proteins whose RNA-binding ability is required for both constitutive and alternative splicing. While members of the SR protein family were once thought to have redundant functions, in-depth biochemical analysis of their RNA-binding abilities has revealed distinct binding profiles for each SR protein, that often lead to either synergistic or antagonistic functions. SR protein family members SRSF1 and SRSF2 are two of the most highly studied RNA-binding proteins. Here we examine the various methods used to differentiate SRSF1 and SRSF2 RNA-binding ability. We discuss the benefits and type of information that can be determined using each method.
authors.affiliation: Irell & Manella Graduate School of Biological Sciences of the City of Hope, Duarte, CA, USA.|Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope, 1500 E. Duarte Road, Duarte, CA, 91010, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: Skrdlant
authors.affiliation: Irell & Manella Graduate School of Biological Sciences of the City of Hope, Duarte, CA, USA. RLin@coh.org.|Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope, 1500 E. Duarte Road, Duarte, CA, 91010, USA. RLin@coh.org.
authors.forename: Ren-Jang
authors.initials: RJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D012313:RNA / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D052156:SELEX Aptamer Technique / Q000379:methods; D000068103:Serine-Arginine Splicing Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: C000595779:SRSF1 protein, human; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors; D012313:RNA
keywords: CLIP-seq; Enzymatic footprinting; RNA affinity chromatography; RNA binding protein; SELEX; SRSF1; SRSF2; Single cell recruitment assay; Solution NMR; Splicing; UV crosslinking