title: Role of single prolonged stress in acquisition of alcohol conditioned place preference in rats.
abstract: OBJECTIVE
  Previous studies showed that exposure to certain types of stressors enhance the rewarding effects of many drugs of abuse, including alcohol; however, no systematic study has investigated the role of single prolonged stress (SPS) in acquisition of alcohol conditioned place preference (CPP). The purpose of this study was to examine whether SPS would facilitate the acquisition of alcohol CPP in rats.
  
  
  METHODS
  Male Sprague-Dawley rats were randomly assigned to either SPS exposure condition or no exposure condition. Freezing behavior and Elevated plus maze (EPM) were employed to evaluate PTSD-like symptoms induced by SPS. Further, using unbiased procedure, CPP conditioning was conducted with alcohol (2g/kg).
  
  
  RESULTS
  SPS significantly enhanced freezing behavior of rats, decreased percentages (%) of both time spent and number of entry into the open arms, and facilitated the acquisition of alcohol CPP without inhibiting rats' activity.
  
  
  CONCLUSIONS
  Our findings suggest that SPS plays an important role in alcohol dependence, and CPP paradigm with SPS may be useful for exploring the rewarding mechanism of alcohol with regard to the interaction between alcohol and post-traumatic stress disorder (PTSD).
authors.affiliation: Department of Psychiatry, First Hospital of the Hebei Medical University, China; Mental Health Institute of the Hebei Medical University, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Psychiatry, First Hospital of the Hebei Medical University, China; Mental Health Institute of the Hebei Medical University, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Psychiatry, First Hospital of the Hebei Medical University, China; Mental Health Institute of the Hebei Medical University, China.
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Psychiatry, First Hospital of the Hebei Medical University, China; Mental Health Institute of the Hebei Medical University, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Psychiatry, First Hospital of the Hebei Medical University, China; Mental Health Institute of the Hebei Medical University, China. Electronic address: ydyywxy@163.corn.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects*; D004597:Electroshock / Q000523:psychology; D000431:Ethanol / Q000494:pharmacology*; D004986:Ether / Q000494:pharmacology; D046353:Immobility Response, Tonic / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D012149:Restraint, Physical / Q000523:psychology; D012201:Reward; D013313:Stress Disorders, Post-Traumatic / Q000523:psychology*; D013312:Stress, Physiological; D013550:Swimming / Q000523:psychology
chemical_list: D004986:Ether; D000431:Ethanol
keywords: Alcohol; Conditioned place preference; Post-traumatic stress disorder; Single prolonged stress