title: Species composition, activity patterns and blood meal analysis of sand fly populations (Diptera: Psychodidae) in the metropolitan region of Thessaloniki, an endemic focus of canine leishmaniasis.
abstract: Species composition, activity patterns and blood meal analysis of sand fly populations were investigated in the metropolitan region of Thessaloniki, North Greece from May to October 2011. Sampling was conducted weekly in 3 different environments (animal facilities, open fields, residential areas) along the outskirts of the city in areas of increased canine leishmania transmission. Six sand fly species (Phlebotomus perfiliewi, Phlebotomus tobbi, Phlebotomus simici, Plebotomus papatasi, Sergentomya minuta and Sergentomya dentata) were identified using both classical and molecular techniques. DNA barcodes were characterized for the first time for two (P. simici and S. dentata) of the six recorded species. Phylogenetic analysis based on the COI gene sequences confirmed the grouping of P. tobbi, P. perniciosus and P. perfiliewi (subgenus Larrousius) and the monophyly of P. simici (subgenus Adlerius). By far the most prevalent species was P. perfiliewi, followed by P. simici and P. tobbi. The largest populations of sand flies were collected from animal facilities, followed by residential areas and open agricultural fields. Peak activity of sand flies overall occurred mid-August to mid-September and then declined sharply in October. Blood meal analysis showed that P. perfiliewi and P. simici feed preferentially on humans (88% & 95%, respectively) but also feed on chickens and goats. When designing a control strategy to alleviate sand fly nuisance in the region of Thessaloniki the following conclusions can be reached from this study: a) August and September are high risk months due to increased sand fly activity levels, b) animal facilities within or adjacent to urban settlements are high risk areas and may act as a maintenance and amplification foci for the vector as well as the parasite, and c) the abundance, ubiquity and feeding behavior of P. perfiliewi and P. simici establishes them as potentially important vectors of Leishmania in the region.
authors.affiliation: European Biological Control Laboratory, USDA-ARS, Tsimiski 43, 54623 Thessaloniki, Greece; Perrotis College, American Farm School, Thessaloniki, Greece. Electronic address: achaskopoulou@ars-ebcl.org.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Chaskopoulou
authors.affiliation: European Biological Control Laboratory, USDA-ARS, Tsimiski 43, 54623 Thessaloniki, Greece; Perrotis College, American Farm School, Thessaloniki, Greece.
authors.forename: Ioannis A
authors.initials: IA
authors.lastname: Giantsis
authors.affiliation: Department of Biology, Ege University, Izmir, Bornova, Turkey.
authors.forename: Samiye
authors.initials: S
authors.lastname: Demir
authors.affiliation: European Biological Control Laboratory, USDA-ARS, Campus International de Baillarguet, Montferrier-sur-Lez 34988, France.
authors.forename: Marie Claude
authors.initials: MC
authors.lastname: Bon
mesh_terms: D000818:Animals; D002645:Chickens / Q000469:parasitology; D058893:DNA Barcoding, Taxonomic; D004199:Disease Vectors; D004283:Dog Diseases / Q000453:epidemiology*; D004285:Dogs; D005247:Feeding Behavior; D006115:Greece / Q000453:epidemiology; D006801:Humans; D007891:Leishmania / Q000235:genetics; D007896:Leishmaniasis / Q000453:epidemiology / Q000662:veterinary*; D010802:Phylogeny; D011157:Population Dynamics; D011576:Psychodidae / Q000145:classification
keywords: Blood meal analysis; Canine leishmaniasis; Greece; Phlebotomine; Sand fly population