title: Comparison of Copy Number of HSF Genes in Two Buffalo Genomes.
abstract: The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations follow Mendelian pattern of inheritance, identification and distribution of these variants between populations can be used to infer the evolutionary history of the species. In this study, we have examined the absolute copy number of four Heat shock factor genes viz. HSF-1, 2, 4, and 5 in two different breeds of buffalo species using real-time PCR. Here, we report that the absolute copy number of HSF2 varies between the two breeds. In contrast no significant difference was observed in the copy number for HSF-1, 4, and 5 between the two breeds. Our results provide evidence for the presence of breed specific differences in HSF2 genomic copy number. This seems to be the first step in delineating the genetic factors underlying environmental adaptation between the two breeds. Nevertheless, a more detailed study is needed to characterize the functional consequence of this variation.
authors.affiliation: a Krishi Vigyan Kendra, ICAR-CIARI , Nimbudera , Andaman and Nicobar Islands.
authors.forename: Shardul Vikram
authors.initials: SV
authors.lastname: Lal
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Ayan
authors.initials: A
authors.lastname: Mukherjee
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Brahma
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Moloya
authors.initials: M
authors.lastname: Gohain
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Mahesh Chandra
authors.initials: MC
authors.lastname: Patra
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Sushil Kumar
authors.initials: SK
authors.lastname: Saini
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Purushottam
authors.initials: P
authors.lastname: Mishra
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Sonika
authors.initials: S
authors.lastname: Ahlawat
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Ramesh C
authors.initials: RC
authors.lastname: Upadhyaya
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Tirtha K
authors.initials: TK
authors.lastname: Datta
authors.affiliation: b Animal Genomics Lab, Animal Biotechnology Center , National Dairy Research Institute , Karnal , Haryana , India.
authors.forename: Sachinandan
authors.initials: S
authors.lastname: De
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D056915:DNA Copy Number Variations / Q000235:genetics*; D016678:Genome; D006360:Heat-Shock Proteins / Q000235:genetics*; D008297:Male; D013737:Testis / Q000737:chemistry; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D006360:Heat-Shock Proteins; D014157:Transcription Factors; D004247:DNA
keywords: Breeds; CNVs; HSF; buffalo; inheritance