title: [PRODUCTION OF CERTAIN PRO- AND ANTI-INFLAMMATORY CYTOKINES DURING STIMULATION BY LIVE AND INACTIVATED LEPTOSPIRA PATHOGENS IN THE MODEL OF HUMAN WHOLE BLOOD].
abstract: OBJECTIVE
  Study of the ability of clinical isolates of leptospira to cause production of certain pro- and antiinflammatory cytokines in the model of human whole blood.
  
  
  METHODS
  Leptospira interrogans strain was taken for the experiment. Cytokine content was determined by a method based on xMAP technology using a standard panel, composed of 9 analytes: TNF-α, MCP-1, IL-8, IL-4, IL-6, IL-10, IL-IRa, IL- 12 (p70), IFN-γ.
  
  
  RESULTS
  An optimal concentration of L. interrogans was selected for stimulation of human whole blood--1 x 10(6) leptospirae/ml. For the first time in the model of human whole blood it was determined, that at early stages of incubation IFN-γ, IL-12(p70), IL-4 and IL-1Ra are more actively produced; at later stages (6 hour incubation)--IL-8 and TNF-α.
  
  
  CONCLUSIONS
  A differential pattern of cytokine production stimulation was shown in the model of human whole blood by live and inactivated leptospirae.
authors.forename: O A
authors.initials: OA
authors.lastname: Petrova
authors.forename: N A
authors.initials: NA
authors.lastname: Stoyanova
authors.forename: N K
authors.initials: NK
authors.lastname: Tokarevich
authors.forename: N A
authors.initials: NA
authors.lastname: Arsentieva
authors.forename: N E
authors.initials: NE
authors.lastname: Lyubimova
authors.forename: A V
authors.initials: AV
authors.lastname: Semenov
authors.forename: Areg A
authors.initials: AA
authors.lastname: Totolyan
mesh_terms: D001773:Blood Cells / Q000276:immunology* / Q000382:microbiology; D054884:Host-Pathogen Interactions; D006358:Hot Temperature; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D053590:Interleukin 1 Receptor Antagonist Protein / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007921:Leptospira interrogans / Q000276:immunology* / Q000472:pathogenicity; D061251:Primary Cell Culture; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: C508598:IL4 protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma