title: Epithelial cell detachment by Porphyromonas gingivalis biofilm and planktonic cultures.
abstract: Porphyromonas gingivalis is present as a biofilm at the sites of periodontal infections. The detachment of gingival epithelial cells induced by P. gingivalis biofilms was examined using planktonic cultures as a comparison. Exponentially grown planktonic cultures or 40-h biofilms were co-incubated with epithelial cells in a 24-well plate for 4 h. Epithelial cell detachment was assessed using imaging. The activity of arginine-gingipain (Rgp) and gene expression profiles of P. gingivalis cultures were examined using a gingipain assay and quantitative PCR, respectively. P. gingivalis biofilms induced significantly higher cell detachment and displayed higher Rgp activity compared to the planktonic cultures. The genes involved in gingipain post-translational modification, but not rgp genes, were significantly up-regulated in P. gingivalis biofilms. The results underline the importance of including biofilms in the study of bacterial and host cell interactions.
authors.affiliation: a Department of Operative Dentistry and Endodontics , Guanghua School of Stomatology, Sun Yat-sen University , Guangzhou , PR China.|b Department of Preventive Dentistry , Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam , Amsterdam , The Netherlands.
authors.forename: Lijia
authors.initials: L
authors.lastname: Huang
authors.affiliation: b Department of Preventive Dentistry , Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam , Amsterdam , The Netherlands.
authors.forename: Cor
authors.initials: C
authors.lastname: van Loveren
authors.affiliation: a Department of Operative Dentistry and Endodontics , Guanghua School of Stomatology, Sun Yat-sen University , Guangzhou , PR China.|c Guangdong Provincial Key Laboratory of Stomatology , Sun Yat-sen University , Guangzhou , PR China.
authors.forename: Junqi
authors.initials: J
authors.lastname: Ling
authors.affiliation: a Department of Operative Dentistry and Endodontics , Guanghua School of Stomatology, Sun Yat-sen University , Guangzhou , PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wei
authors.affiliation: b Department of Preventive Dentistry , Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam , Amsterdam , The Netherlands.
authors.forename: Wim
authors.initials: W
authors.lastname: Crielaard
authors.affiliation: b Department of Preventive Dentistry , Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam , Amsterdam , The Netherlands.|c Guangdong Provincial Key Laboratory of Stomatology , Sun Yat-sen University , Guangzhou , PR China.
authors.forename: Dong Mei
authors.initials: DM
authors.lastname: Deng
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods; D018441:Biofilms / Q000254:growth & development*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D000080867:Gingipain Cysteine Endopeptidases; D006801:Humans; D010510:Periodontal Diseases / Q000175:diagnosis / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000201:enzymology / Q000472:pathogenicity; D011499:Protein Processing, Post-Translational
chemical_list: D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D003546:Cysteine Endopeptidases
keywords: Cytotoxicity; detachment; gingipain; gingival epithelial cells; glycosyltransferase; quantitative PCR