title: Effect of antifreeze glycoprotein 8 supplementation during vitrification on the developmental competence of bovine oocytes.
abstract: The purpose of this study was to investigate the effect of antifreeze glycoprotein 8 (AFGP8) supplementation during vitrification on the survival, fertilization, and embryonic development of bovine oocytes and the underlying molecular mechanism(s). Survival, fertilization, early embryonic development, apoptosis, DNA double-strand breaks, reactive oxygen species levels, meiotic cytoskeleton assembly, chromosome alignment, and energy status of mitochondria were measured in the present experiments. Compared with that in the nonsupplemented group; survival, monospermy, blastocyst formation rates, and blastomere counts were significantly higher in the AFGP8-supplemented animals. Oocytes of the latter group also presented fewer double-strand breaks and lower cathepsin B and caspase activities. Rates of normal spindle organization and chromosome alignment, actin filament impairment, and mitochondrial distribution were significantly higher in the AFGP8-supplemented group. In addition, intracellular reactive oxygen species levels significantly decreased in the AFGP8-supplemented groups, maintaining a higher ΔΨm than that in the nonsupplemented group. Taken together, these results indicated that supplementation with AFGP8 during vitrification has a protective effect on bovine oocytes against chilling injury.
authors.affiliation: Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea; Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Kwon
authors.affiliation: Division of Magnetic Resonance, Korea Basic Science Institute, Chungbuk, Republic of Korea.
authors.forename: Mija
authors.initials: M
authors.lastname: Ahn
authors.affiliation: Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.affiliation: Division of Magnetic Resonance, Korea Basic Science Institute, Chungbuk, Republic of Korea.
authors.forename: Jeong Kyu
authors.initials: JK
authors.lastname: Bang
authors.affiliation: Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea; Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, China. Electronic address: nhkim@chungbuk.ac.kr.
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
mesh_terms: D000818:Animals; D021301:Antifreeze Proteins / Q000494:pharmacology*; D001755:Blastocyst; D002417:Cattle / Q000502:physiology*; D018929:Cell Culture Techniques; D015925:Cryopreservation / Q000662:veterinary*; D046149:Embryo Culture Techniques; D047108:Embryonic Development; D005307:Fertilization in Vitro / Q000662:veterinary; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology*; D058989:Vitrification
chemical_list: D021301:Antifreeze Proteins
keywords: Antifreeze glycoprotein 8; Cytoskeleton integrity; Embryonic development; Fertilization; Oocyte; Vitrification