title: Kordiimonas sediminis sp. nov., isolated from a sea cucumber culture pond.
abstract: A marine bacterium, designated strain N39(T), was isolated from a sediment sample collected at a sea cucumber culture pond in Weihai, China. Cells of strain N39(T) were observed to be Gram-stain negative, facultatively anaerobic, motile rods showing catalase and oxidase negative reactions. Strain N39(T) was found to grow optimally at pH 8.0-8.5, 35-37 °C and in the presence of approximately 3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain N39(T) belongs to the genus Kordiimonas in the family Kordiimonadaceae, appearing closely related to Kordiimonas lacus JCM 16261(T) (95.9 %), Kordiimonas aquimaris MEBiC06554(T) (95.1 %), Kordiimonas gwangyangensis JCM 12864(T) (94.2 %) and Kordiimonas aestuarii 101-1(T) (93.8 %). Ubiquinone 10 (Q-10) was found to be the major respiratory quinone. The dominant cellular fatty acids were identified as iso-C17:1 ω9c, iso-C17:0, iso-C15:0 and C17:1 ω6c. The predominant polar lipids were found to be phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G + C content of strain N39(T) is 50.8 %. On the basis of genotypic, chemotaxonomic and phenotypic data, strain N39(T) is concluded to represent a novel species within the genus Kordiimonas, for which the name Kordiimonas sediminis sp. nov. is proposed. The type strain is N39(T) (=KCTC 42590(T) = MCCC 1H00112(T)).
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China.
authors.forename: Heng-Xi
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China.
authors.forename: Jin-Xin
authors.initials: JX
authors.lastname: Zhao
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China.|State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Chen
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, 264209, People's Republic of China. duzongjun@sdu.edu.cn.|State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China. duzongjun@sdu.edu.cn.
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Du
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D000818:Animals; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology; D010641:Phenotype; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012616:Sea Cucumbers / Q000382:microbiology*; D012623:Seawater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA gene; Kordiimonas sediminis sp. nov.; Polyphasic taxonomy; Sea cucumber culture pond