title: Rapid Recognition and Isolation of Live Colon Cancer Stem Cells by Using Metabolic Labeling of Azido Sugar and Magnetic Beads.
abstract: New approach for colon cancer stem cells (CSCs) recognition and isolation is reported. Colon CSCs are responsible for colonic tumor growth, metastasis, and resistance for radio-/chemotherapies. An accurate identification and isolation method is critical for understanding and characterization of these cells. In our work, we recognized CSCs' population from colon cancer cells by using metabolic labeling of azido sugar based on the quiescent nature of these cells, which differed fundamentally from previously described methods by using specific cellular markers to recognize and isolate CSCs. Later the putative CSCs were isolated by using commercially available magnetic beads. The isolated cells population had much higher sphere formation efficiency, soft-agar colony formation efficiency, and an mRNA level of colon stem cells marker Lgr5 than the leftover population. Our method provides a new avenue and a general strategy for recognition and isolation of CSCs, which shows great potential for further use in both the fundamental research of CSCs and clinical tests.
authors.affiliation: Key laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University , 620 Xi Chang'an Street, Xi'an, Shaanxi 710119, People's Republic of China.
authors.forename: Lingbo
authors.initials: L
authors.lastname: Sun
authors.affiliation: Key laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University , 620 Xi Chang'an Street, Xi'an, Shaanxi 710119, People's Republic of China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Fu
authors.affiliation: Key laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University , 620 Xi Chang'an Street, Xi'an, Shaanxi 710119, People's Republic of China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University , 620 Xi Chang'an Street, Xi'an, Shaanxi 710119, People's Republic of China.
authors.forename: Xinrui
authors.initials: X
authors.lastname: Duan
authors.affiliation: Key laboratory of Applied Surface and Colloid Chemistry Ministry of Education, Key laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University , 620 Xi Chang'an Street, Xi'an, Shaanxi 710119, People's Republic of China.
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D001386:Azides / Q000737:chemistry* / Q000378:metabolism; D001710:Biotin / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D002469:Cell Separation; D003110:Colonic Neoplasms / Q000473:pathology*; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D060328:Magnetic Phenomena; D008863:Microspheres; D014411:Neoplastic Stem Cells / Q000473:pathology*
chemical_list: D001386:Azides; D002241:Carbohydrates; D001710:Biotin