title: One pot phytosynthesis of gold nanoparticles using Genipa americana fruit extract and its biological applications.
abstract: In this article, rapid one pot synthesis of gold nanoparticles (GNPs) using an eco-friendly extract of Genipa americana L. fruit is described. Electrospray ionization mass spectrometry (ESI-MS) and Fourier transform infrared (FTIR) spectroscopic studies demonstrated that small molecules such as genipin, genipaol, geniposide and ranolazine can act as reducer as well as stabilizers. The monodispersed, spherical GNPs were further characterized by UV-vis spectroscopy at λmax=535 nm, transmission electron microscopy (TEM), dynamic light scattering (DLS) and X-ray diffraction (XRD) analysis. This synthetic approach offers a greener and alternate route to the preparation of GNPs free from toxic chemical components and stable for 6-7 months under room temperature. The green synthesized GNPs showed weak antioxidant efficacy against 1,1-diphenyl-2-picrylhydrazyl and no cytotoxicity against A-549 and HeLa human cancer cell lines, from lung and cervix. This study opens a new industrial scope of G. americana fruit in nanoscience and as surface modified GNPs can be developed into a successful drug carrier for future pharmaceutical products.
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador. Electronic address: krmbraj@gmail.com.
authors.forename: Brajesh
authors.initials: B
authors.lastname: Kumar
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Kumari
authors.initials: K
authors.lastname: Smita
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Luis
authors.initials: L
authors.lastname: Cumbal
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador; Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología, C.P. 07360 Mexico City, Mexico.
authors.forename: Javier
authors.initials: J
authors.lastname: Camacho
authors.affiliation: Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología, C.P. 07360 Mexico City, Mexico.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Hernández-Gallegos
authors.affiliation: Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología, C.P. 07360 Mexico City, Mexico.
authors.forename: María
authors.initials: M
authors.lastname: de Guadalupe Chávez-López
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Grijalva
authors.affiliation: Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador.
authors.forename: Kleber
authors.initials: K
authors.lastname: Andrade
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000067493:Dynamic Light Scattering; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D055772:Green Chemistry Technology; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D010936:Plant Extracts; D006046:Gold
keywords: Cancer cell line; FTIR; Genipa americana L.; Gold nanoparticles; TEM; XRD