title: Safety profile of the intravenous administration of brain-targeted stable nucleic acid lipid particles.
abstract: In a clinical setting, where multiple administrations of the therapeutic agent are usually required to improve the therapeutic outcome, it is crucial to assess the immunogenicity of the administered nanoparticles. In this data work, we investigated the safety profile of the repeated intravenous administration of brain-targeted stable nucleic acid lipid particles (RVG-9r-targeted SNALPs). To evaluate local activation of the immune system, we performed analysis of mouse tissue homogenates and sections from cerebellum. To investigate peripheral activation of the immune system, we used serum of mice that were intravenously injected with RVG-9r-targeted SNALPs. These data are related and were discussed in the accompanying research article entitled "Intravenous administration of brain-targeted stable nucleic acid lipid particles alleviates Machado-Joseph disease neurological phenotype" (Conceição et al., in press) [1].
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal; Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Mariana
authors.initials: M
authors.lastname: Conceição
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Mendonça
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal.
authors.forename: Clévio
authors.initials: C
authors.lastname: Nóbrega
authors.affiliation: IBILI - Institute of Biomedical Research in Light and Image, Faculty of Medicine, University of Coimbra, 3000-354 Coimbra, Portugal.
authors.forename: Célia
authors.initials: C
authors.lastname: Gomes
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal.
authors.forename: Pedro
authors.initials: P
authors.lastname: Costa
authors.affiliation: Graduate School of Medicine, Gunma University, Maebashi, Gunma 371-8511, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hirai
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal; Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: João Nuno
authors.initials: JN
authors.lastname: Moreira
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Lima
authors.affiliation: Center of Excellence in Infectious Diseases, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA.
authors.forename: N
authors.initials: N
authors.lastname: Manjunath
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Rua Larga, 3004-504 Coimbra, Portugal; Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Luís Pereira
authors.initials: LP
authors.lastname: de Almeida