title: Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection.
abstract: Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have rectified this anomaly by genome engineering. The variant created (CRG20291) represents an ideal control strain for virulence assays of ClosTron mutants.
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Kelly
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
authors.forename: Yen Kuan
authors.initials: YK
authors.lastname: Ng
authors.affiliation: Nottingham Digestive Disease Centre, NIHR Biomedical Research Unit, The University of Nottingham, University Park, Nottingham, United Kingdom.
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Cartman
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
authors.forename: Mark M
authors.initials: MM
authors.lastname: Collery
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
authors.forename: Alan
authors.initials: A
authors.lastname: Cockayne
authors.affiliation: Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom; Nottingham Digestive Disease Centre, NIHR Biomedical Research Unit, The University of Nottingham, University Park, Nottingham, United Kingdom. Electronic address: nigel.minton@nottingham.ac.uk.
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Minton
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D016360:Clostridioides difficile / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D003412:Cricetulus / Q000235:genetics* / Q000382:microbiology; D004195:Disease Models, Animal; D004761:Enterocolitis, Pseudomembranous / Q000188:drug therapy / Q000382:microbiology* / Q000401:mortality / Q000473:pathology; D015870:Gene Expression; D005813:Genes, Synthetic; D005818:Genetic Engineering; D016680:Genome, Bacterial; D006801:Humans; D008780:Methyltransferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D016019:Survival Analysis; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase
keywords: Clindamycin sensitivity; Clostridium difficile; Genome engineering; Hamster model; ermB