title: Interaction of an anti-cancer photosensitizer with a genomic DNA: From base pair specificity and thermodynamic landscape to tuning the rate of detergent-sequestered dissociation.
abstract: A detailed characterization of the binding interaction of a potent cancer cell photosensitizer, norharmane (NHM) with a genomic DNA (herring sperm; hsDNA) is undertaken with particular emphasis on deciphering the strength, mode, dynamics, energetics and kinetics of binding. A major focus of the study underlies a successful exploration of the concept of detergent-sequestered dissociation of drug from the drug-DNA complex. Biophysical techniques such as absorption, steady-state and time-resolved fluorescence spectroscopy, circular dichroism, DNA helix melting, stopped-flow fluorescence kinetics and calorimetry have been used. A primarily intercalative mode of binding of NHM with DNA is shown. However, the overall interaction is governed by more than one type of binding forces. We demonstrate that the essential prerequisite of a slower dissociation rate of drug from DNA helix is achieved by tenable choice surfactants. Our results also highlight an effective tunability of the rate of dissociation of the DNA-intercalated drug via detergent-sequestration. A detailed isothermal titration calorimetric study unveils the key role of hydrophobic force underlying NHM-hsDNA association. This is further substantiated by the enthalpy-entropy compensation behavior. The major entropic contribution in detergent-induced dissociation of NHM from NHM-hsDNA complex is also demonstrated. Our results present not only a comprehensive structural and thermodynamic profile, base pair specificity, association kinetics for binding of NHM with DNA but also explore the thermodynamic and kinetic aspects of dissociation of bound drug. Characterization and tuning of the essential prerequisites for a drug to be efficient in anti-cancer functionality bear direct and widespread significance in contemporary global research.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 462066, Madhya Pradesh, India. Electronic address: bpaul@iiserb.ac.in.
authors.forename: Bijan K
authors.initials: BK
authors.lastname: Paul
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 462066, Madhya Pradesh, India.
authors.forename: Narayani
authors.initials: N
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhauri, Bhopal 462066, Madhya Pradesh, India. Electronic address: saptarshi@iiserb.ac.in.
authors.forename: Saptarshi
authors.initials: S
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D020029:Base Pairing; D002243:Carbolines / Q000737:chemistry*; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D003902:Detergents / Q000737:chemistry*; D005399:Fishes; D008297:Male; D017319:Photosensitizing Agents / Q000737:chemistry*; D013094:Spermatozoa / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D000970:Antineoplastic Agents; D002243:Carbolines; D003902:Detergents; D017319:Photosensitizing Agents; D004247:DNA; C010262:norharman
keywords: Detergent-sequestered drug dissociation; Intercalation; Interplay of various binding forces; NHM-hsDNA interaction; Thermodynamics; Tuning the rate of dissociation