title: Missense mutations in the signal peptide of the porcine GH gene affect cellular synthesis and secretion.
abstract: BACKGROUND
  In previous investigations, we have demonstrated the mutations in the signal peptide of porcine GH gene were associated with the body size.
  
  
  METHODS
  In this study, the fusion gene expression vectors which consisted of eight signal peptide mutants of GH gene and EGFP gene were constructed according to three missense mutations (p.Val9Ala, p.Gln22Arg and p.Asp25Gly), and they were transfected into the GH3 cell line.
  
  
  RESULTS
  The inhibition levels of EGFP gene transcriptions with different signal peptide mutants were significantly different. Typically, the allelic variants carrying Val in codon nine showed higher protein synthesis (P < 0.05), and the allelic variants carrying neutral Gln in codon 22 and Gly in codon 25 showed higher secretion proportion (P < 0.05) compared with the other groups as assessed by western blotting. In silico RNA folding prediction indicated that the mutations gave rise to different RNA secondary structures, suggesting that they might affect translation and protein synthesis.
  
  
  CONCLUSIONS
  We conclude that the missense mutations within the signal sequence influence the expression and the secretion of the protein. To the best of our knowledge, this is the first report addressing the functional consequences of the mutations in the signal peptide of porcine GH gene.
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.|Five-Star Animal Health Pharmaceutical Factory of Jilin Province, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Songcai
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Haoyu
authors.initials: H
authors.lastname: Fu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Lv
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Nie
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. haolinlin@jlu.edu.cn.
authors.forename: Linlin
authors.initials: L
authors.lastname: Hao
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D020125:Mutation, Missense; D059370:RNA Folding / Q000235:genetics; D051381:Rats; D034421:Sus scrofa; D013552:Swine
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D013006:Growth Hormone
keywords: Cellular secretion; GH gene; RNA fold; Signal peptide