title: Detection of mumps virus genotype H in two previously vaccinated patients from Mexico City.
abstract: Infections caused by mumps virus (MuV) have been successfully prevented through vaccination; however, in recent years, an increasing number of mumps outbreaks have been reported within vaccinated populations. In this study, MuV was genotyped for the first time in Mexico. Saliva samples were obtained from two previously vaccinated patients in Mexico City who had developed parotitis. Viral isolation was carried out in Vero cells, and the SH and HN genes were amplified by RT-PCR. Amplicons were sequenced and compared to a set of reference sequences to identify the MuV genotype.
authors.affiliation: Laboratory of Virology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, Casco de Santo Tomás, 11340, Mexico, D.F., Mexico.
authors.forename: Alberto
authors.initials: A
authors.lastname: Del Valle
authors.affiliation: Laboratory of Virology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, Casco de Santo Tomás, 11340, Mexico, D.F., Mexico.
authors.forename: Alí A
authors.initials: AA
authors.lastname: García
authors.affiliation: Laboratory of Virology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, Casco de Santo Tomás, 11340, Mexico, D.F., Mexico. bbarron@ipn.mx.
authors.forename: Blanca L
authors.initials: BL
authors.lastname: Barrón
mesh_terms: D000818:Animals; D002648:Child; D002522:Chlorocebus aethiops; D004196:Disease Outbreaks; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D017720:Molecular Epidemiology; D009107:Mumps / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D009108:Mumps Vaccine / Q000494:pharmacology; D009109:Mumps virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D014709:Vero Cells; D055815:Young Adult
chemical_list: D009108:Mumps Vaccine
keywords: Genotype H mumps; Mexico; Mumps detection; Vaccinated patients