title: Optogenetic Tools for Confined Stimulation in Deep Brain Structures.
abstract: Optogenetics has emerged in the past decade as a technique to modulate brain activity with cell-type specificity and with high temporal resolution. Among the challenges associated with this technique is the difficulty to target a spatially restricted neuron population. Indeed, light absorption and scattering in biological tissues make it difficult to illuminate a minute volume, especially in the deep brain, without the use of optical fibers to guide light. This work describes the design and the in vivo application of a side-firing optical fiber adequate for delivering light to specific regions within a brain subcortical structure.
authors.affiliation: École Polytechnique de Montréal, CP 6079, succ. Centre-ville, Montréal, QC, Canada, H3C 3A7.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Castonguay
authors.affiliation: Laboratoire des Neurosciences de la Vision, École d'optométrie, Université de Montréal, CP 6128, succ. Centre-ville, Montréal, QC, Canada, H3C 3J7.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Thomas
authors.affiliation: École Polytechnique de Montréal, CP 6079, succ. Centre-ville, Montréal, QC, Canada, H3C 3A7.|Research Center, Montreal Heart Institute, 5000 Bélanger Street, Montréal, QC, Canada, H1T 1C8.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lesage
authors.affiliation: Laboratoire des Neurosciences de la Vision, École d'optométrie, Université de Montréal, CP 6128, succ. Centre-ville, Montréal, QC, Canada, H3C 3J7. christian.casanova@umontreal.ca.
authors.forename: Christian
authors.initials: C
authors.lastname: Casanova
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology* / Q000528:radiation effects; D000075402:Channelrhodopsins; D004867:Equipment Design; D007834:Lasers; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D055100:Optical Fibers; D062308:Optogenetics / Q000295:instrumentation* / Q000379:methods; D010775:Photic Stimulation / Q000295:instrumentation / Q000379:methods
chemical_list: D000075402:Channelrhodopsins
keywords: Deep brain stimulation; Optogenetics; Side-firing optical fiber