title: Dual-emissive fluorescence measurements of hydroxyl radicals using a coumarin-activated silica nanohybrid probe.
abstract: This work reports a novel dual-emissive fluorescent probe based on dye hybrid silica nanoparticles for ratiometric measurement of the hydroxyl radical (˙OH). In the probe sensing system, the blue emission of coumarin dye (coumarin-3-carboxylic acid, CCA) immobilized on the nanoparticle surface is selectively enhanced by ˙OH due to the formation of a coumarin hydroxylation product with strong fluorescence, whereas the emission of red fluorescent dye encapsulated in the silica nanoparticle is insensitive to ˙OH as a self-referencing signal, and so the probe provides a good quantitative analysis based on ratiometric fluorescence measurement with a detection limit of 1.65 μM. Moreover, the probe also shows high selectivity for ˙OH determination against metal ions, other reactive oxygen species and biological species. More importantly, it exhibits low cytotoxicity and high biocompatibility in living cells, and has been successfully used for cellular imaging of ˙OH, showing its promising application for monitoring of intracellular ˙OH signaling events.
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. shwang@iim.ac.cn kzhang@iim.ac.cn and Department of Materials Science and Engineering, University of Science & Technology of China, Hefei, Anhui 230026, China and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Saisai
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. shwang@iim.ac.cn kzhang@iim.ac.cn and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. shwang@iim.ac.cn kzhang@iim.ac.cn and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, CB 3290, Chapel Hill, NC 27599, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. shwang@iim.ac.cn kzhang@iim.ac.cn and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Mingtai
authors.initials: M
authors.lastname: Sun
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. shwang@iim.ac.cn kzhang@iim.ac.cn and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. shwang@iim.ac.cn kzhang@iim.ac.cn and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Yehan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui 230031, China. shwang@iim.ac.cn kzhang@iim.ac.cn and State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
authors.forename: Suhua
authors.initials: S
authors.lastname: Wang
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry*; D057230:Limit of Detection; D057054:Molecular Imaging; D053758:Nanoparticles / Q000737:chemistry*; D010539:Permeability; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D003374:Coumarins; D017665:Hydroxyl Radical; D012822:Silicon Dioxide; C030123:coumarin