title: The C2H2-type transcription factor, FlbC, is involved in the transcriptional regulation of Aspergillus oryzae glucoamylase and protease genes specifically expressed in solid-state culture.
abstract: Aspergillus oryzae produces a large amount of secreted proteins in solid-state culture, and some proteins such as glucoamylase (GlaB) and acid protease (PepA) are specifically produced in solid-state culture, but rarely in submerged culture. From the disruption mutant library of A. oryzae transcriptional regulators, we successfully identified a disruption mutant showing an extremely low production level of GlaB but a normal level of α-amylase production. This strain was a disruption mutant of the C2H2-type transcription factor, FlbC, which is reported to be involved in the regulation of conidiospore development. Disruption mutants of other upstream regulators comprising a conidiation regulatory network had no apparent effect on GlaB production in solid-state culture. In addition to GlaB, the production of acid protease in solid-state culture was also markedly decreased by flbC disruption. Northern blot analyses revealed that transcripts of glaB and pepA were significantly decreased in the flbC disruption strain. These results suggested that FlbC is involved in the transcriptional regulation of genes specifically expressed under solid-state cultivation conditions, possibly independent of the conidiation regulatory network.
authors.affiliation: Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan.
authors.forename: Midori
authors.initials: M
authors.lastname: Yoshimura
authors.affiliation: Noda Institute for Scientific Research, 399 Noda, Noda, Chiba, 278-0037, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Noda Institute for Scientific Research, 399 Noda, Noda, Chiba, 278-0037, Japan.
authors.forename: Yasuji
authors.initials: Y
authors.lastname: Koyama
authors.affiliation: Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shintani
authors.affiliation: Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan. gomi@biochem.tohoku.ac.jp.
authors.forename: Katsuya
authors.identifier: http://orcid.org/0000-0003-3463-8072
authors.initials: K
authors.lastname: Gomi
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D014157:Transcription Factors; D005087:Glucan 1,4-alpha-Glucosidase; D010447:Peptide Hydrolases
keywords: Acid protease; Aspergillus oryzae; Conidiospore development; Gene expression regulation; Glucoamylase; Solid-state culture; Transcription factor FlbC