title: Serum and Muscle Metabolomics for the Prediction of Ultimate pH, a Key Factor for Chicken-Meat Quality.
abstract: Variations in muscle glycogen storage are highly correlated with variations in meat ultimate pH (pHu), a key factor for poultry meat quality. A total of two chicken lines were divergently selected on breast pHu to understand the biological basis for variations in meat quality (i.e., the pHu- and the pHu+ lines that are characterized by a 17% difference in muscle glycogen content). The effects of this selection on bird metabolism were investigated by quantifying muscle metabolites by high-resolution NMR ((1)H and (31)P) and serum metabolites by (1)H NMR. A total of 20 and 26 discriminating metabolites between the two lines were identified by orthogonal partial least-squares discriminant analysis (OPLS-DA) in the serum and muscle, respectively. There was over-representation of carbohydrate metabolites in the serum and muscle of the pHu- line, consistent with its high level of muscle glycogen. However, the pHu+ line was characterized by markers of oxidative stress and muscle catabolism, probably because of its low level of energy substrates. After OPLS-DA multiblock analysis, a metabolic set of 15 high-confidence biomarkers was identified that could be used to predict the quality of poultry meat after validation on an independent population.
authors.affiliation: URA, INRA , F-37380 Nouzilly, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Beauclercq
authors.affiliation: Département d'Analyses Chimique Biologique et Médicale, PPF Analyse des Systèmes Biologiques, Université François-Rabelais , F-37000 Tours, France.
authors.forename: Lydie
authors.initials: L
authors.lastname: Nadal-Desbarats
authors.affiliation: URA, INRA , F-37380 Nouzilly, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Hennequet-Antier
authors.affiliation: URA, INRA , F-37380 Nouzilly, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Collin
authors.affiliation: URA, INRA , F-37380 Nouzilly, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Tesseraud
authors.affiliation: ITAVI - Institut Technique de l'Aviculture , F-37380 Nouzilly, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Bourin
authors.affiliation: URA, INRA , F-37380 Nouzilly, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Le Bihan-Duval
authors.affiliation: URA, INRA , F-37380 Nouzilly, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Berri
mesh_terms: D000818:Animals; D002645:Chickens; D016002:Discriminant Analysis; D004734:Energy Metabolism / Q000502:physiology; D006003:Glycogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008460:Meat / Q000032:analysis*; D055432:Metabolomics; D015233:Models, Statistical; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress; D013045:Species Specificity
chemical_list: D006003:Glycogen
keywords: 1H NMR; 31P NMR; biomarkers; chicken; meat quality; metabolomics; ultimate pH