title: Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma.
abstract: The surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer, with the exception being a power level of 250 W. The wettability of the modified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. It was revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma.
authors.affiliation: Department of Experimental Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
authors.forename: D S
authors.initials: DS
authors.lastname: Syromotina
authors.affiliation: Department of Experimental Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia; Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, Germany. Electronic address: rsurmenev@gmail.com.
authors.forename: R A
authors.initials: RA
authors.lastname: Surmenev
authors.affiliation: Department of Experimental Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Surmeneva
authors.affiliation: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Boyandin
authors.affiliation: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russia.
authors.forename: E D
authors.initials: ED
authors.lastname: Nikolaeva
authors.affiliation: Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45117 Essen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Prymak
authors.affiliation: Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45117 Essen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Epple
authors.affiliation: Technical Chemistry II and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45141 Essen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Ulbricht
authors.affiliation: Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Oehr
authors.affiliation: Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russia.
authors.forename: T G
authors.initials: TG
authors.lastname: Volova
mesh_terms: D000818:Animals; D006885:Hydroxybutyrates / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells; D058626:Plasma Gases / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D017155:Wettability; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D008567:Membranes, Artificial; D058626:Plasma Gases; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Plasma; Polar component; Surface energy; Surface treatment; poly-3-hydroxybutyrate membranes