title: Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies.
abstract: An easy, low-cost and fast wet processing-based method named ASB-SANS (Auxiliary Solvent-Based Sublimation-Aided NanoStructuring) has been used to fabricate poly(l-lactic acid) (PLLA) highly ordered and hierarchically organized 2D fibrillar patterns, with fiber widths between 40 and 500 nm and lengths exceeding tens of microns. A clear contact guidance effect of these nanofibrillar scaffolds with respect to HeLa and NIH-3T3 cells growth has been observed, on top of an overall good viability. For NIH-3T3 pronounced elongation of the cells was observed, as well as a remarkable ability of the patterns to guide the extension of pseudopodia. Moreover, SEM imaging revealed filopodia stemming from both sides of the pseudopodia and aligned with the secondary PLLA nanofibrous structures created by the ASB-SANS procedure. These results validate ASB-SANS as a technique capable to provide biocompatible 2D nanofibrillar patterns suitable for studying phenomena of contact guidance (and, more in general, the behavior of cells onto nanofibrous scaffolds), at very low costs and in an extremely easy way, accessible to virtually any laboratory.
authors.affiliation: Department of Engineering and Architecture, Univ. of Trieste - V. Valerio 10, 34100 Trieste, Italy.
authors.forename: John
authors.initials: J
authors.lastname: Mohanraj
authors.affiliation: Department of Engineering and Architecture, Univ. of Trieste - V. Valerio 10, 34100 Trieste, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Puzzi
authors.affiliation: Sincrotrone Trieste SCpA, s.s. 14 km 163,5, 34149 Basovizza, TS, Italy.
authors.forename: Ennio
authors.initials: E
authors.lastname: Capria
authors.affiliation: Sincrotrone Trieste SCpA, s.s. 14 km 163,5, 34149 Basovizza, TS, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Corvaglia
authors.affiliation: Sincrotrone Trieste SCpA, s.s. 14 km 163,5, 34149 Basovizza, TS, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Casalis
authors.affiliation: University of Colorado Denver AMC, 13001 E17th, Aurora, CO 80045, USA.
authors.forename: Luisa
authors.initials: L
authors.lastname: Mestroni
authors.affiliation: Department of Engineering and Architecture, Univ. of Trieste - V. Valerio 10, 34100 Trieste, Italy.
authors.forename: Orfeo
authors.initials: O
authors.lastname: Sbaizero
authors.affiliation: Department of Engineering and Architecture, Univ. of Trieste - V. Valerio 10, 34100 Trieste, Italy; Sincrotrone Trieste SCpA, s.s. 14 km 163,5, 34149 Basovizza, TS, Italy; CNR Nano S3, Via Campi 213/A, 41125 Modena, Italy. Electronic address: afraleoni@units.it.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Fraleoni-Morgera
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry* / Q000633:toxicity; D011091:Polyesters / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C033616:poly(lactide)
keywords: ASB-SANS; Contact guidance; HeLa; NIH-3T3; PLLA; Scaffold