title: In Vitro Anticancer Activity of Au, Ag Nanoparticles Synthesized Using Commelina nudiflora L. Aqueous Extract Against HCT-116 Colon Cancer Cells.
abstract: Recently, metal nanoparticles have been getting great medical and social interests due to their potential physico-chemical properties such as higher affinity, low molecular weight, and larger surface area. The biosynthesized gold and silver nanoparticles are spherical, triangular in shape with an average size of 24-150 nm as reported in our earlier studies. The biological properties of synthesized gold and silver nanoparticles are demonstrated in this paper. The different in vitro assays such as MTT, flow cytometry, and reverse transcription polymerase chain reaction (RT-qPCR) techniques were used to evaluate the in vitro anticancer properties of synthesized metal nanoparticles. The biosynthesized gold and silver nanoparticles have shown reduced cell viability and increased cytotoxicity in HCT-116 colon cancer cells with IC50 concentration of 200 and 100 μg/ml, respectively. The flow cytometry experiments revealed that the IC50 concentrations of gold and silver nanoparticle-treated cells that have significant changes were observed in the sub-G1 cell cycle phase compared with the positive control. Additionally, the relative messenger RNA (mRNA) gene expressions of HCT-116 cells were studied by RT-qPCR techniques. The pro-apoptotic genes such as PUMA (++), Caspase-3 (+), Caspase-8 (++), and Caspase-9 (++) were upregulated in the treated HCT-116 cells compared with cisplatin. Overall, these findings have proved that the synthesized gold and silver nanoparticles could be potent anti-colon cancer drugs.
authors.affiliation: Mammalian Cell Culture Technology Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia. kpalaselvam@yahoo.co.in.
authors.forename: Palaniselvam
authors.initials: P
authors.lastname: Kuppusamy
authors.affiliation: Kulliyyah of Dentistry, International Islamic University Malaysia, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia.
authors.forename: Solachuddin J A
authors.initials: SJ
authors.lastname: Ichwan
authors.affiliation: Kulliyyah of Pharmacy, Department of Pharmaceutical Chemistry, International Islamic University Malaysia, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia.
authors.forename: Putri Nur Hidayah
authors.initials: PN
authors.lastname: Al-Zikri
authors.affiliation: Kulliyyah of Pharmacy, Department of Pharmaceutical Chemistry, International Islamic University Malaysia, Bandar Indera Mahkota, 25200, Kuantan, Pahang, Malaysia.
authors.forename: Wastuti Hidayati
authors.initials: WH
authors.lastname: Suriyah
authors.affiliation: Department of Biochemistry, Rural Development and Administration, National Institute of Animal Science, Suwon-si, Gyeonggi-do, 441706, South Korea.
authors.forename: Ilavenil
authors.initials: I
authors.lastname: Soundharrajan
authors.affiliation: Mammalian Cell Culture Technology Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia.
authors.forename: Natanamurugaraj
authors.initials: N
authors.lastname: Govindan
authors.affiliation: Mammalian Cell Culture Technology Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia.
authors.forename: Gaanty Pragas
authors.initials: GP
authors.lastname: Maniam
authors.affiliation: Mammalian Cell Culture Technology Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia.
authors.forename: Mashitah M
authors.initials: MM
authors.lastname: Yusoff
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D034822:Commelina / Q000737:chemistry*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D012834:Silver; D006046:Gold
keywords: Apoptosis; Gold nanoparticles; HCT-116; RT-qPCR; Silver nanoparticles