title: Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex.
abstract: Optogenetics is rapidly gaining acceptance as a preferred method to study specific neuronal cell types using light. Optogenetic neuromodulation requires the introduction of a cell-specific viral vector encoding for a light activating ion channel or ion pump and the utilization of a system to deliver light stimulation to brain. Here, we describe a two-part methodology starting with a procedure to inject an optogenetic AAV virus into rat cortex followed by a second procedure to surgically implant an optical cannula for light delivery to the deeper cortical layers.
authors.affiliation: Biophysics Department, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Pawela
authors.affiliation: Radiology Department, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.
authors.forename: Edgar
authors.initials: E
authors.lastname: DeYoe
authors.affiliation: Electrical Engineering Department, University of Wisconsin-Milwaukee, 3200 N. Cramer St., EMS 1181, Milwaukee, WI, 53211, USA. pashaie@uwm.edu.
authors.forename: Ramin
authors.initials: R
authors.lastname: Pashaie
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000502:physiology* / Q000528:radiation effects / Q000601:surgery; D004867:Equipment Design; D018014:Gene Transfer Techniques / Q000295:instrumentation; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D007473:Ion Channels / Q000235:genetics; D016623:Ion Pumps / Q000235:genetics; D008027:Light; D062308:Optogenetics / Q000295:instrumentation / Q000379:methods*; D010775:Photic Stimulation / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D012243:Rhodopsin / Q000235:genetics; D014780:Viruses / Q000235:genetics*
chemical_list: D007473:Ion Channels; D016623:Ion Pumps; D012243:Rhodopsin
keywords: Intracranial injection; Neural stimulation; Neuromodulation; Optical cannula; Optogenetics; Rat brain