title: Filamin C: a novel component of the KCNE2 interactome during hypoxia.
abstract: OBJECTIVE
  KCNE2 encodes for the potassium voltage-gated channel, KCNE2. Mutations in KCNE2 have been associated with long-QT syndrome (LQTS). While KCNE2 has been extensively studied, the functions of its C-terminal domain remain inadequately described. Here, we aimed to elucidate the functions of this domain by identifying its protein interactors using yeast two-hybrid analysis.
  
  
  METHODS
  The C-terminal domain of KCNE2 was used as bait to screen a human cardiac cDNA library for putative interacting proteins. Co-localisation and co-immunoprecipitation analyses were used for verification.
  
  
  RESULTS
  Filamin C (FLNC) was identified as a putative interactor with KCNE2. FLNC and KCNE2 co-localised within the cell, however, a physical interaction was only observed under hypoxic conditions.
  
  
  CONCLUSIONS
  The identification of FLNC as a novel KCNE2 ligand not only enhances current understanding of ion channel function and regulation, but also provides valuable information about possible pathways likely to be involved in LQTS pathogenesis.
authors.affiliation: DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, SA MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
authors.forename: Annika
authors.initials: A
authors.lastname: Neethling
authors.affiliation: DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, SA MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
authors.forename: Jomien
authors.initials: J
authors.lastname: Mouton
authors.affiliation: Department of Physiological Sciences, Faculty of Science, Stellenbosch University, Stellenbosch, South Africa.
authors.forename: Ben
authors.initials: B
authors.lastname: Loos
authors.affiliation: DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, SA MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
authors.forename: Valerie
authors.initials: V
authors.lastname: Corfield
authors.affiliation: DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, SA MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
authors.forename: Carin
authors.initials: C
authors.lastname: de Villiers
authors.affiliation: DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, SA MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
authors.forename: Craig
authors.initials: C
authors.lastname: Kinnear
mesh_terms: D015687:Cell Hypoxia; D002460:Cell Line; D064448:Filamins / Q000378:metabolism*; D006329:Heart Conduction System / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D008133:Long QT Syndrome / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics*
chemical_list: D064448:Filamins; C489619:KCNE2 protein, human; D024642:Potassium Channels, Voltage-Gated