title: Effects of Methane-Rich Saline on the Capability of One-Time Exhaustive Exercise in Male SD Rats.
abstract: OBJECTIVE
  To explore the effects of methane-rich saline (CH4 saline) on the capability of one-time exhaustive exercise in male SD rats.
  
  
  METHODS
  Thirty rats were equally divided into to three groups at random: control group (C), placebo group (P) and methane saline group (M). Rats in M group underwent intraperitoneal injection of CH4 saline, and the other two groups simultaneously underwent intraperitoneal injection of normal saline. Then, the exercise capability of rats was tested through one-time exhaustive treadmill exercise except C group. Exercise time and body weight were recorded before and after one-time exhaustive exercise. After exhaustive exercise, the blood and gastrocnemius samples were collected from all rats to detect biochemical parameters in different methods.
  
  
  RESULTS
  It was found that the treadmill running time was significantly longer in rats treated with CH4 saline. At the same time, CH4 saline reduced the elevation of LD and UN in blood caused by one-time exhaustive exercise. The low level of blood glucose induced by exhaustive exercise was also normalized by CH4 saline. Also CH4 saline lowered the level of CK in plasma. Furthermore, this research indicated that CH4 saline markedly increased the volume of T-AOC in plasma and alleviated the peak of TNF-α in both plasma and gastrocnemius. From H&E staining, CH4 saline effectively improved exercise-induced structural damage in gastrocnemius.
  
  
  CONCLUSIONS
  CH4 saline could enhance exercise capacity in male SD rats through increase of glucose aerobic oxidation, improvement of metabolic clearance and decrease of exhaustive exercise-induced gastrocnemius injury.
authors.affiliation: Department of Kinesiology, Shanghai University of Sport, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xin
authors.affiliation: Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Kinesiology, Shanghai University of Sport, Shanghai, China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Lou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003402:Creatine Kinase / Q000097:blood; D016207:Cytokines / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008697:Methane / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects; D009584:Nitrogen / Q000097:blood; D009195:Peroxidase / Q000097:blood; D010805:Physical Conditioning, Animal; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors; D014508:Urea / Q000097:blood
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D016207:Cytokines; D019344:Lactic Acid; D012965:Sodium Chloride; D008315:Malondialdehyde; D014508:Urea; D009195:Peroxidase; D013482:Superoxide Dismutase; D003402:Creatine Kinase; D009584:Nitrogen; D008697:Methane