title: Bjcul, a snake venom lectin, modulates monocyte-derived macrophages to a pro-inflammatory profile in vitro.
abstract: Macrophages are cells of high plasticity and can act in different ways to ensure that the appropriate immune response remains controlled. This study shows the effects of the C-type Bothrops jararacussu venom lectin (BJcuL) on the activation of human macrophages derived from the U937 cell line. BJcuL binds on the cell surface, and this event is inhibited by its specific carbohydrate. It induced phagocytosis and production of H2O2, and expression of antigen presentation molecules. It also enhanced the production of TNF-α, GM-CSF and IL-6 by macrophages and indirectly induced T cells to an increased production of TNF-α, IFN-γ and IL-6 in the presence of LPS. Our results suggest that BJcuL can modulate macrophage functional activation towards an M1 state.
authors.affiliation: Pós-graduação em Ciências da Saúde, Escola de Medicina, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Curitiba, PR CEP 80215-901, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: Dias-Netipanyj
authors.affiliation: Núcleo de Tecnologia Celular, Escola de Medicina, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Curitiba, PR CEP 80215-901, Brazil.
authors.forename: L M
authors.initials: LM
authors.lastname: Boldrini-Leite
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Rua Francisco H. dos Santos, 100, Caixa Postal 19031, Curitiba, PR CEP 81531-980, Brazil.
authors.forename: E S
authors.initials: ES
authors.lastname: Trindade
authors.affiliation: Pós-graduação em Ciências da Saúde, Escola de Medicina, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Curitiba, PR CEP 80215-901, Brazil.
authors.forename: A N
authors.initials: AN
authors.lastname: Moreno-Amaral
authors.affiliation: Pós-graduação em Ciências da Saúde, Escola de Medicina, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Curitiba, PR CEP 80215-901, Brazil. Electronic address: selene.e@pucpr.br.
authors.forename: S
authors.initials: S
authors.lastname: Elifio-Esposito
mesh_terms: D018920:Coculture Techniques; D003435:Crotalid Venoms / Q000633:toxicity*; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009000:Monocytes / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D020298:U937 Cells
chemical_list: C498078:BJcuL lectin, Bothrops jararacussu; D003435:Crotalid Venoms; D016207:Cytokines; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D006861:Hydrogen Peroxide
keywords: Bothrops jararacussu venom; C-type lectin; Immunomodulation; M1/Th1; Macrophages