title: Spectrofluorimetric Method for Quantification of Triazine Herbicides in Agricultural Matrices.
abstract: A spectrofluorimetric method for determination of triazine herbicides was developed. The method involves reaction of ammonical 2-cyanoacetamide with the herbicide. The net fluorescent intensity (FI) of the product was measured at 376 nm using 330 nm as excitation wavelength. A linear relationship between concentration and FI was found in the range of 0.3 - 10 μg mL(-1) for atrazine and 0.2 - 10 μg mL(-1) for terbutryn. The LOD and LOQ were found to be 0.07 ± 0.023 μg mL(-1) and 0.23 ± 0.023 μg mL(-1), respectively, with %RSD <12.1% for atrazine and 0.027 ± 0.009 μg mL(-1) and 0.091 ± 0.009 μg mL(-1), respectively, with %RSD <5% for terbutryn. The %recoveries of the subject triazines from soil and wheat grains were found in the range of 90.0 ± 0.14 to 96.0 ± 0.15% for atrazine and 95.0 ± 0.05 to 98 ± 0.02% for terbutryn.
authors.affiliation: Institute of Chemical Sciences, University of Peshawar.
authors.forename: Mian
authors.initials: M
authors.lastname: Muhammad
authors.forename: Jasmin
authors.initials: J
authors.lastname: Shah
authors.forename: M Rasul
authors.initials: MR
authors.lastname: Jan
authors.forename: Behisht
authors.initials: B
authors.lastname: Ara
authors.forename: Mariam
authors.initials: M
authors.lastname: Mahabat Khan
authors.forename: Abid
authors.initials: A
authors.lastname: Jan
mesh_terms: D002523:Edible Grain / Q000737:chemistry*; D006540:Herbicides / Q000032:analysis*; D009570:Nitriles / Q000737:chemistry; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014227:Triazines / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*
chemical_list: D006540:Herbicides; D009570:Nitriles; D012989:Soil Pollutants; D014227:Triazines; C031594:2-cyanoacetamide