title: Smash and DASH with Cas9.
abstract: DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.
authors.affiliation: Department of Genome Sciences, University of Washington, 3720 15th Ave NE, Seattle, WA, 98195, USA. vramani@uw.edu.
authors.forename: Vijay
authors.initials: V
authors.lastname: Ramani
authors.affiliation: Department of Genome Sciences, University of Washington, 3720 15th Ave NE, Seattle, WA, 98195, USA. shendure@uw.edu.|Howard Hughes Medical Institute, 3720 15th Ave NE, Seattle, WA, 98195, USA. shendure@uw.edu.
authors.forename: Jay
authors.initials: J
authors.lastname: Shendure
mesh_terms: D064113:CRISPR-Cas Systems / Q000235:genetics; D003141:Communicable Diseases / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012313:RNA / Q000235:genetics; D017393:RNA Editing / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA