title: Proteome identification of the silkworm middle silk gland.
abstract: To investigate the functional differentiation among the anterior (A), middle (M), and posterior (P) regions of silkworm middle silk gland (MSG), their proteomes were characterized by shotgun LC-MS/MS analysis with a LTQ-Orbitrap mass spectrometer. To get better proteome identification and quantification, triplicate replicates of mass spectrometry analysis were performed for each sample. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (Vizcaíno et al., 2014) [1] via the PRIDE partner repository (Vizcaino, 2013) [2] with the dataset identifier PXD003371. The peptide identifications that were further processed by PeptideProphet program in Trans-Proteomic Pipeline (TPP) after database search with Mascot software were also available in .XML format files. Data presented here are related to a research article published in Journal of Proteomics by Li et al. (2015) [3].
authors.affiliation: Institute of Developmental and Regenerative Biology, Hangzhou Normal University, Hangzhou 310036, PR China; College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Li
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Lu-Peng
authors.initials: LP
authors.lastname: Ye
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Jia-Qian
authors.initials: JQ
authors.lastname: Che
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Zheng-Ying
authors.initials: ZY
authors.lastname: You
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Wang
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
authors.forename: Bo-Xiong
authors.initials: BX
authors.lastname: Zhong
keywords: Bombyx mori; Label-free; Middle silk gland; Shotgun proteomics; Silk protein synthesis