title: Application of low vacuum scanning electron microscopy for Papanicolaou-stained slides for cytopathology examinations.
abstract: Papanicolaou (Pap)-stained slides are usually observed using a transmitted light microscope for cytopathology. However, progress in pathological examinations has created a need for new diagnostic tools, because cytopathological preparations do not allow additional examinations without a loss of specimen, unlike histopathology. Low-vacuum scanning electron microscopy (LVSEM) can reveal the surface topography at an ultrastructual resolution without metal coating. The aim of this study was to determine the conditions required for observing Pap-stained slides of oral smears using LVSEM without any loss of specimen and to reexamine the same slides again using light microscopy, while preserving the cytopathological information.
authors.affiliation: Electron Microscope Laboratory, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan Section of Molecular Pathology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan appatare1970@z7.keio.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yano
authors.affiliation: Section of Molecular Pathology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Yurie
authors.initials: Y
authors.lastname: Soejima
authors.affiliation: Section of Molecular Pathology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
authors.forename: Motoji
authors.initials: M
authors.lastname: Sawabe
mesh_terms: D004847:Epithelial Cells / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009061:Mouth Mucosa / Q000166:cytology*; D065006:Papanicolaou Test / Q000379:methods*; D010772:Phosphotungstic Acid / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods
chemical_list: D010772:Phosphotungstic Acid
keywords: Papanicolaou-stained slides; cytopathology; low vacuum scanning electron microscope; oral mucosa; phosphotungstic acid