title: An inactivated whole-virus porcine parvovirus vaccine protects pigs against disease but does not prevent virus shedding even after homologous virus challenge.
abstract: Inactivated whole-virus vaccines against porcine parvovirus (PPV) can prevent disease but not infection and virus shedding after heterologous virus challenge. Here, we showed that the same is true for a homologous challenge. Pregnant sows were vaccinated with an experimental inactivated vaccine based on PPV strain 27a. They were challenged on day 40 of gestation with the virulent porcine parvovirus PPV-27a from which the vaccine was prepared (homologous challenge). On day 90 of gestation, the fetuses from vaccinated sows were protected against disease, while the fetuses of the non-vaccinated sows (control group) exhibited signs of parvovirus disease. All gilts, whether vaccinated or not vaccinated, showed a boost of PPV-specific antibodies indicative of virus infection and replication. Low DNA copy numbers, but not infectious virus, could be demonstrated in nasal or rectal swabs of immunized sows, but high copy numbers of challenge virus DNA as well as infectious virus could both be demonstrated in non-vaccinated sows.
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, Universität Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
authors.forename: Tessa
authors.initials: T
authors.lastname: Foerster
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, Universität Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.|Laboratory of Virology, Faculty of Veterinary Medicine, Rio Grande do Sul Federal University, Avenida Bento Gonçalves 9090, 91540-000 Porto Alegre, Brazil.
authors.forename: André Felipe
authors.initials: AF
authors.lastname: Streck
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, Universität Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Speck
authors.affiliation: IDT Biologika GmbH, PF 400214, 06855 Rosslau, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Selbitz
authors.affiliation: IDT Biologika GmbH, PF 400214, 06855 Rosslau, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lindner
authors.affiliation: Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, Universität Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Truyen
mesh_terms: D000818:Animals; D009297:Nasal Mucosa / Q000821:virology; D010322:Parvoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D028323:Parvovirus, Porcine / Q000276:immunology* / Q000302:isolation & purification; D012007:Rectum / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017201:Virus Shedding
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines