title: The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132.
abstract: miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.
authors.affiliation: Division of Cancer Research, Jacqui Wood Cancer Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.
authors.forename: Judit
authors.initials: J
authors.lastname: Remenyi
authors.affiliation: Faculty of Engineering and Information Technology, Centre for Health Technologies, University of Technology Sydney, NSW 2007, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Bajan
authors.affiliation: Division of Cancer Research, Jacqui Wood Cancer Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.
authors.forename: Frances V
authors.initials: FV
authors.lastname: Fuller-Pace
authors.affiliation: Division of Cell Signaling and Immunology, College of Life Sciences, Wellcome Trust Building, University of Dundee, Dundee, UK.
authors.forename: J Simon C
authors.initials: JS
authors.lastname: Arthur
authors.affiliation: Faculty of Engineering and Information Technology, Centre for Health Technologies, University of Technology Sydney, NSW 2007, Australia.
authors.forename: Gyorgy
authors.initials: G
authors.lastname: Hutvagner
mesh_terms: D000818:Animals; D002460:Cell Line; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional; D015854:Up-Regulation
chemical_list: C546976:MIRN132 microRNA, mouse; C554518:MIRN212 microRNA, mouse; D035683:MicroRNAs; C577630:Ddx17 protein, mouse; D053487:DEAD-box RNA Helicases