title: Hyaluronic acid embedded cellulose acetate phthlate core/shell nanoparticulate carrier of 5-fluorouracil.
abstract: Aim of this research was to prepare hyaluronic acid-modified-cellulose acetate phthalate (HAC) core shell nanoparticles (NPs) of 5-fluorouracil (5-FU). HAC copolymer was synthesized and confirmed by fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. HAC NPs with 5-FU were prepared using HAC copolymer and compared with 5-FU loaded cellulose acetate phthalate (CAP) NPs. NPs were characterized by atomic force microscopy (AFM), particle size, zeta potential, polydispersity index, entrapment efficiency, in-vitro release, differential scanning calorimetry (DSC) and X-ray diffraction (XRD). HAC NPs were found slower release (97.30% in 48h) than (99.25% in 8h) CAP NPs. In cytotoxicity studies, showed great cytotoxic potential of 5-FU loaded HAC NPs in A549, MDA-MD-435 and SK-OV-3 cancer cellline. HAC NPs showing least hemolytic than CAP NPs and 5-FU. Area under curve (AUC), maximum plasma concentration (Cmax), mean residence time (MRT) and time to reach maximum plasma concentration Tmax), were observed 4398.1±7.90μgh/mL, 145.45±2.25μg/L, 45.74±0.25h, 72±0.50h, respectively of HAC NPs and 119.92±1.78μgh/mL, 46.38±3.42μg/L, 1.2±0.25h, 0.5±0.02h were observed in plain 5-FU solution. In conclusion, HAC NPs is effective deliver carrier of 5-FU for lung cancer.
authors.affiliation: Drug Delivery and Nanotechnology Laboratories, Department of Pharmaceutics, Guru Ramdas Khalsa Institute of Science and Technology Pharmacy, Kukrikheda, Barela 482003, Madhya Pradesh, India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Garg
authors.affiliation: Drug Delivery and Nanotechnology Laboratories, Department of Pharmaceutics, Guru Ramdas Khalsa Institute of Science and Technology Pharmacy, Kukrikheda, Barela 482003, Madhya Pradesh, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Rai
authors.affiliation: Drug Delivery and Nanotechnology Laboratories, Department of Pharmaceutics, Guru Ramdas Khalsa Institute of Science and Technology Pharmacy, Kukrikheda, Barela 482003, Madhya Pradesh, India.
authors.forename: Santram
authors.initials: S
authors.lastname: Lodhi
authors.affiliation: Drug Delivery and Nanotechnology Laboratories, Department of Pharmaceutics, Guru Ramdas Khalsa Institute of Science and Technology Pharmacy, Kukrikheda, Barela 482003, Madhya Pradesh, India.
authors.forename: Alok Pal
authors.initials: AP
authors.lastname: Jain
authors.affiliation: Drug Delivery and Nanotechnology Laboratories, Department of Pharmaceutics, Guru Ramdas Khalsa Institute of Science and Technology Pharmacy, Kukrikheda, Barela 482003, Madhya Pradesh, India. Electronic address: aweshyadav@gmail.com.
authors.forename: Awesh K
authors.initials: AK
authors.lastname: Yadav
mesh_terms: D000272:Adipates / Q000737:chemistry; D000818:Animals; D045744:Cell Line, Tumor; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D065546:Drug Liberation; D005472:Fluorouracil / Q000737:chemistry* / Q000493:pharmacokinetics; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D051381:Rats
chemical_list: D000272:Adipates; D004337:Drug Carriers; D002482:Cellulose; C011708:cellulose acetate phthalate; D006820:Hyaluronic Acid; D005472:Fluorouracil; C010011:adipic dihydrazide
keywords: Cellular cytotoxicity; Cellulose acetate phthalate; Hyaluronic acid; Nanoparticles