title: Multifunctional Coating Improves Cell Adhesion on Titanium by using Cooperatively Acting Peptides.
abstract: Promotion of cell adhesion on biomaterials is crucial for the long-term success of a titanium implant. Herein a novel concept is highlighted combining very stable and affine titanium surface adhesive properties with specific cell binding moieties in one molecule. A peptide containing L-3,4-dihydroxyphenylalanine was synthesized and affinity to titanium was investigated. Modification with a cyclic RGD peptide and a heparin binding peptide (HBP) was realized by an efficient on-resin combination of Diels-Alder reaction with inverse electron demand and Cu(I) catalyzed azide-alkyne cycloaddition. The peptide was fluorescently labeled by thiol Michael addition. Conjugating the cyclic RGD and HBP in one peptide gave improved spreading, proliferation, viability, and the formation of well-developed actin cytoskeleton and focal contacts of osteoblast-like cells.
authors.affiliation: Institut für Biochemie, Universität Leipzig, Brüderstrasse 34, 04103, Leipzig, Germany.
authors.forename: Mareen
authors.initials: M
authors.lastname: Pagel
authors.affiliation: Institut für Biochemie, Universität Leipzig, Brüderstrasse 34, 04103, Leipzig, Germany.|Institut für Bioanalytische Chemie, Universität Leipzig, Deutscher Platz 5, 04103, Leipzig, Germany.
authors.forename: Rayk
authors.initials: R
authors.lastname: Hassert
authors.affiliation: Leibniz-Institut für Oberflächenmodifizierung (IOM), and Wilhelm-Ostwald-Institut für Physikalische und Theoretische Chemie, Universität Leipzig, Permoserstrasse 15, 04318, Leipzig, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: John
authors.affiliation: Deutsches Krebsforschungszentrum, Department Medizinische Physik in der Radiologie, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Braun
authors.affiliation: Deutsches Krebsforschungszentrum, Department Medizinische Physik in der Radiologie, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Wießler
authors.affiliation: Leibniz-Institut für Oberflächenmodifizierung (IOM), and Wilhelm-Ostwald-Institut für Physikalische und Theoretische Chemie, Universität Leipzig, Permoserstrasse 15, 04318, Leipzig, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Abel
authors.affiliation: Institut für Biochemie, Universität Leipzig, Brüderstrasse 34, 04103, Leipzig, Germany. abeck-sickinger@uni-leipzig.de.
authors.forename: Annette G
authors.initials: AG
authors.lastname: Beck-Sickinger
mesh_terms: D002448:Cell Adhesion; D002851:Chromatography, High Pressure Liquid; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D014025:Titanium / Q000737:chemistry*
chemical_list: D010455:Peptides; D014025:Titanium
keywords: DOPA; cell adhesion; click chemistry; peptides; surface chemistry