title: Effective mRNA Inhibition in PANC-1 Cells in Vitro Mediated via an mPEG-SeSe-PEI Delivery System.
abstract: RNA interference (RNAi)-mediated gene therapy is a promising approach to cure various diseases. However, developing an effective, safe, specific RNAi delivery system remains a major challenge. In this study, a novel redox-responsive polyetherimide (PEI)-based nanovector, mPEG-SeSe-PEI, was developed and its efficacy evaluated. We prepared three mPEG-SeSe-PEI vector candidates for small interfering glyceraldehyde-3-phosphate dehydrogenase (siGADPH) and determined their physiochemical properties and transfection efficiency using flow cytometry and PEG11.6-SeSe-PEI polymer. We investigated the silencing efficacy of GADPH mRNA expression in PANC-1 cells and observed that PEG11.6-SeSe-PEI/siGADPH (N/P ratio=10) polyplexes possessed the appropriate size and zeta-potential and exhibited excellent in vitro gene silencing effects with the least cytotoxicity in PANC-1 cells. In conclusion, we present PEG11.6-SeSe-PEI as a potential therapeutic gene delivery system for small interfering RNA (siRNA).
authors.affiliation: Department of Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Yajie
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Qin
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Lantian
authors.initials: L
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Zheng
authors.forename: Yulian
authors.initials: Y
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000235:genetics*; D006801:Humans; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000737:chemistry; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D034622:RNA Interference; D012333:RNA, Messenger / Q000037:antagonists & inhibitors; D034741:RNA, Small Interfering / Q000008:administration & dosage*
chemical_list: D011108:Polymers; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C433673:polyetherimide; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)