title: In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications.
abstract: OBJECTIVE
  This study was carried out to identify and outline the degree of relationship between immunophenotyped macrophages expressing CD163 and PDGF-B in cyclosporine-A, phenytoin, and nifedipine-induced gingival overgrowth.
  
  
  METHODS
  Eighty adult male albino rats were selected and divided into four equal groups. Group I received no treatment. Rats of groups II, III, and IV were administered cyclosporine-A, phenytoin, and nifedipine, respectively. Routine tissue processing was carried out for staining with CD163 and PDGF-B. The results of this study were analyzed statistically.
  
  
  RESULTS
  Group I exhibited score 0 gingival overgrowth while group II yielded score 3 with blunt and bulbous gingival crests. Rats of group III showed score 2 with knife edge and group IV revealed less pronounced gingival overgrowth and mostly the gingival crest was knife edge. Group II had the highest mean value for CD163 while group I showed the lowest value. In addition, group II had the highest mean value for PDGF-B while group I showed the lowest value. Statistically, there was an overall significant difference between the studied groups as well as between each two groups.
  
  
  CONCLUSIONS
  Strong association exists between immunophenotyped macrophages expressing CD163 and PDGF-B in GO induced by these medications. In addition, CD163 and PDGF-B upregulated in cyclosporine-A-induced GO compared to phenytoin and nifedipine medications.
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Almergib University, Libya.
authors.forename: Amina J
authors.initials: AJ
authors.lastname: Almahrog
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mansoura University, Egypt.
authors.forename: Lobna R S
authors.initials: LR
authors.lastname: Radwan
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mansoura University, Egypt.
authors.forename: Rehab R
authors.initials: RR
authors.lastname: El-Zehery
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Mansoura University, Egypt.
authors.forename: Mohamed I
authors.initials: MI
authors.lastname: Mourad
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mansoura University, Egypt; Department of Oral Biology, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamasa, Mansoura, Egypt.
authors.forename: Mohammed E
authors.initials: ME
authors.lastname: Grawish
keywords: CD163; Cyclosporine-A; Nifedipine; PDGF-B; Phenytoin