title: Effects of prunetin on the proteolytic activity, secretion and gene expression of MMP-3 in vitro and production of MMP-3 in vivo.
abstract: We investigated whether prunetin affects the proteolytic activity, secretion, and gene expression of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee joint to evaluate the potential chondroprotective eff ect of prunetin. Rabbit articular chondrocytes were cultured in a monolayer, and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and ADAMTS-5. In rabbit articular chondrocytes, the effects of prunetin on IL-1β-induced secretion and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The eff ect of prunetin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) prunetin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5; (2) prunetin inhibited the secretion and proteolytic activity of MMP-3; (3) prunetin suppressed the production of MMP-3 protein in vivo. These results suggest that prunetin can regulate the gene expression, secretion, and proteolytic activity of MMP-3, by directly acting on articular chondrocytes.
authors.affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
authors.forename: Dae Cheol
authors.initials: DC
authors.lastname: Nam
authors.affiliation: Department of Orthopedic Surgery, School of Medicine, Chungnam National University, Daejeon 35015, Korea.
authors.forename: Bo Kun
authors.initials: BK
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Orthopedic Surgery, School of Medicine, Chungnam National University, Daejeon 35015, Korea.
authors.forename: Hyun-Dae
authors.initials: HD
authors.lastname: Shin
authors.affiliation: Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 35015, Korea.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
authors.affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
authors.forename: Sun-Chul
authors.initials: SC
authors.lastname: Hwang
keywords: Chondrocyte; Metalloproteinase; Osteoarthritis; Prunetin