title: Altered expression of neuropeptide Y receptors caused by focal cortical dysplasia in human intractable epilepsy.
abstract: Focal cortical dysplasia (FCD) is a common cause of pharmacologically-intractable epilepsy, however, the precise mechanisms underlying the epileptogenicity of FCD remains to be determined. Neuropeptide Y (NPY), an endogenous anticonvulsant in the central nervous system, plays an important role in the regulation of neuronal excitability. Increased expression of NPY and its receptors has been identified in the hippocampus of patients with mesial temporal lobe epilepsy, presumed to act as an endogenous anticonvulsant mechanism. Therefore, we investigated whether expression changes in NPY receptors occurs in patients with FCD. We specifically investigated the expression of seizure-related NPY receptor subtypes Y1, Y2, and Y5 in patients with FCD versus autopsy controls. We found that Y1R and Y2R were up-regulated at the mRNA and protein levels in the temporal and frontal lobes in FCD lesions. By contrast, there was no significant change in either receptor detected in parietal lesions. Notably, overexpression of Y5R was consistently observed in all FCD lesions. Our results demonstrate the altered expression of Y1R, Y2R and Y5R occurs in FCD lesions within the temporal, frontal and parietal lobe. Abnormal NPY receptor subtype expression may be associated with the onset and progression of epileptic activity and may act as a therapeutic candidate for the treatment of refractory epilepsy caused by FCD.
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, P.R. China.|Beijing Key Laboratory in Epilepsy, Beijing, P.R. China.|Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, P.R. China.|Beijing Key Laboratory in Epilepsy, Beijing, P.R. China.|Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, P.R. China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Neural Regeneration and Repair, Department of Neurobiology, Beijing Institute for Brain Disorders, Capital Medical University, Beijing, P.R. China.
authors.forename: Hanjiang
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Qi
authors.affiliation: Beijing Key Laboratory in Epilepsy, Beijing, P.R. China.|Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, P.R. China.
authors.forename: Tianfu
authors.initials: T
authors.lastname: Li
authors.affiliation: Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, P.R. China.|Beijing Key Laboratory of Neural Regeneration and Repair, Department of Neurobiology, Beijing Institute for Brain Disorders, Capital Medical University, Beijing, P.R. China.|Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
authors.forename: Zhiqing David
authors.initials: ZD
authors.lastname: Xu
authors.affiliation: Department of Functional Neurosurgery, Sanbo Brain Hospital, Capital Medical University, Beijing, P.R. China.|Beijing Key Laboratory in Epilepsy, Beijing, P.R. China.|Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing, P.R. China.
authors.forename: Guo-Ming
authors.initials: GM
authors.lastname: Luan
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D000069279:Drug Resistant Epilepsy / Q000209:etiology / Q000378:metabolism*; D004827:Epilepsy / Q000150:complications / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D065703:Malformations of Cortical Development, Group I / Q000150:complications / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D055815:Young Adult
chemical_list: D017476:Receptors, Neuropeptide Y
keywords: Pathology Section; focal cortical dysplasia; neuropeptide Y Y1 receptor; neuropeptide Y Y2 receptor; neuropeptide Y Y5 receptor