title: Green synthesis of silver nanoparticles using methanolic root extracts of Diospyros paniculata and their antimicrobial activities.
abstract: Since the discovery and subsequent widespread use of antibiotics, a variety of bacterial species of human and animal origin have developed numerous mechanisms that render bacteria resistant to some, and in certain cases to nearly all antibiotics, thereby limiting the treatment options and compromising effective therapy. In the present study, the green synthesis of nanoparticles is carried out by the reduction of silver acetate in the presence of crude methanolic root extracts of Diospyros paniculata, a member of family Ebenaceae. The UV-Vis absorption spectrum of the biologically reduced reaction mixture showed the surface plasmon peak at 428 nm, a characteristic peak of silver nanoparticles. X-ray diffraction (XRD) analysis confirmed the face-centered cubic crystalline structure of metallic silver. The average diameter of Ag NPs is about 17 nm from Transmission Electron Microscopy (TEM) which is in good agreement with the average crystallite size (19 nm) calculated from XRD analysis. Further the study has been extended to the antimicrobial activity against test pathogenic Gram (+), Gram (-) bacterial and fungal strains. The biologically synthesized silver nanoparticles showed promising activity against all the tested pathogenic strains and the activity has been enhanced with the increased dose levels.
authors.affiliation: Advanced Analytical Laboratory, DST-PURSE Programme, Andhra University, Visakhapatnam 530003, India.
authors.forename: N Hanumanta
authors.initials: NH
authors.lastname: Rao
authors.affiliation: Department of Microbiology, College of Science and Technology, Andhra University, Visakhapatnam 530003, India.
authors.forename: Lakshmidevi
authors.initials: L
authors.lastname: N
authors.affiliation: Advanced Analytical Laboratory, DST-PURSE Programme, Andhra University, Visakhapatnam 530003, India; Department of Materials Science and Engineering, Chungnam National University, Daeduk Science Town, 305-764, Daejeon, Korea.
authors.forename: S V N
authors.initials: SV
authors.lastname: Pammi
authors.affiliation: DST-INSPIRE Faculty, Department of Metallurgical Engineering & Materials Science, Indian Institute of Technology Bombay, Mumbai 400076, India.
authors.forename: Pratap
authors.initials: P
authors.lastname: Kollu
authors.affiliation: GITAM Institute of Pharmacy, GITAM University, Visakhapatnam.
authors.forename: Ganapaty
authors.initials: G
authors.lastname: S
authors.affiliation: Department of Microbiology, College of Science and Technology, Andhra University, Visakhapatnam 530003, India. Electronic address: lmkandregula@gmail.com.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: P
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D029788:Diospyros / Q000737:chemistry* / Q000378:metabolism; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D000432:Methanol / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D012834:Silver; D000432:Methanol
keywords: Antimicrobial properties; Root extract; Silver nanoparticles