title: The role of biodegradable engineered random polycaprolactone nanofiber scaffolds seeded with nestin-positive hair follicle stem cells for tissue engineering.
abstract: BACKGROUND
  Tissue engineering is a new approach to reconstruction and/or regeneration of lost or damaged tissue. The purpose of this study was to fabricate the polycaprolactone (PCL) random nanofiber scaffold as well as evaluation of the cell viability, adhesion, and proliferation of rat nestin-positive hair follicle stem cells (HFSCs) in the graft material using electrospun PCL nanofiber scaffold in regeneration medicine.
  
  
  METHODS
  The bulge HFSCs was isolated from rat whiskers and cultivated in Dulbecco's modified Eagle's medium/F12. To evaluate the biological nature of the bulge stem cells, flow cytometry using nestin, CD34 and K15 antibodies was performed. Electrospinning was used for the production of PCL nanofiber scaffolds. Furthermore, scanning electron microscopy (SEM) for HFSCs attachment, infiltration, and morphology, 3-(4, 5-di-methylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay for cell viability and cytotoxicity, tensile strength of the scaffolds mesh, and histology analysis were used.
  
  
  RESULTS
  Flow cytometry showed that HFSCs were nestin and CD34 positive but K15 negative. The results of the MTT assay showed cell viability and cell proliferation of the HFSCs on PCL nanofiber scaffolds. SEM microscopy photographs indicated that HFSCs are attached and spread on PCL nanofiber scaffolds. Furthermore, tensile strength of the scaffolds mesh was measured.
  
  
  CONCLUSIONS
  The results of this study revealed that modified PCL nanofiber scaffolds are suitable for HFSCs seeding, attachment, and proliferation. Furthermore, HFSCs are attached and proliferated on PCL nanofiber scaffolds.
authors.affiliation: Department of Anatomy, School of Medicine, Stem Cell Technology Research Centre, Karaj, Iran; Department of Anatomy, School of Medicine, Alborz University of Medical Science, Karaj, Iran.
authors.forename: Abazar
authors.initials: A
authors.lastname: Yari
authors.affiliation: Department of Medical Genetics, Stem Cell Technology Research Centre, Karaj, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Teimourian
authors.affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Fardin
authors.initials: F
authors.lastname: Amidi
authors.affiliation: Department of Anatomy, School of Medicine, Stem Cell Technology Research Centre, Karaj, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Bakhtiyari
authors.affiliation: Department of Anatomy, School of Medicine, Stem Cell Technology Research Centre, Karaj, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Heidari
authors.affiliation: Department of Anatomy, School of Medicine, Stem Cell Technology Research Centre, Karaj, Iran.
authors.forename: Nayereh
authors.initials: N
authors.lastname: Sajedi
authors.affiliation: Department of Anatomy, School of Medicine, Stem Cell Technology Research Centre, Karaj, Iran.
authors.forename: Sanaz Joulai
authors.initials: SJ
authors.lastname: Veijouye
authors.affiliation: Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Centre, Karaj, Iran.
authors.forename: Masumeh
authors.initials: M
authors.lastname: Dodel
authors.affiliation: Department of Anatomy, School of Medicine, Stem Cell Technology Research Centre, Karaj, Iran; Physiology Research Centre, Iran University of Medical Science, Karaj, Iran; Anti-microbial Resistance Research Centre, Iran University of Medical Science, Karaj, Iran.
authors.forename: Maliheh
authors.initials: M
authors.lastname: Nobakht
keywords: Hair follicle; nestin; polycaprolactone; scaffold; stem cell; tissue engineering