title: Absorption and Urinary Excretion of Peptides after Collagen Tripeptide Ingestion in Humans.
abstract: Collagen tripeptide (CTP) is a collagen hydrolysate containing a high concentration of tripeptides with a Gly-X-Y sequence, such as Gly-Pro-Hyp. To test the effects of this preparation, we compared the absorption of peptides in humans after ingestion of a tripeptide fraction of CTP (CTP-100), a CTP preparation containing ca. 50% Gly-X-Y tripeptides (CTP-50), and a collagen peptide that did not contain tripeptides (CP). The postprandial levels of Gly-Pro-Hyp and Pro-Hyp in the plasma increased in those subjects who ingested CTP-100 and CTP-50, and were higher with greater Gly-Pro-Hyp ingestion. This demonstrated that collagen hydrolysates were efficiently absorbed when the collagen was ingested in the tripeptide form. Gly-Pro-Hyp and Pro-Hyp were also found in the urine after ingestion of CTP-100 or CTP-50. Similar to the results for the plasma concentration, the urinary excretion of Gly-Pro-Hyp and Pro-Hyp was also dependent on the amount of Gly-Pro-Hyp ingested. This indicates that ingested Gly-Pro-Hyp and generated Pro-Hyp were relatively stable in the body and were transported to the urine in the peptide form. The concentration of Hyp-Gly in the plasma was low after the ingestion of CP and CTP-100 but higher after the ingestion of CTP-50. Overall, our results suggest that tripeptides derived from collagen are absorbed efficiently by the body.
authors.affiliation: Central Research Institute, Jellice Co., Ltd.
authors.forename: Shoko
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Deguchi
authors.forename: Masamichi
authors.initials: M
authors.lastname: Onuma
authors.forename: Noriaki
authors.initials: N
authors.lastname: Numata
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sakai
mesh_terms: D000328:Adult; D024042:Collagen Type I / Q000737:chemistry*; D004435:Eating; D006801:Humans; D007408:Intestinal Absorption; D008297:Male; D009842:Oligopeptides / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D010455:Peptides / Q000652:urine*
chemical_list: D024042:Collagen Type I; D009842:Oligopeptides; D010455:Peptides; C088188:glycyl-prolyl-hydroxyproline