title: Studies on biomarkers of oxidative stress and associated genotoxicity and histopathology in Channa punctatus from heavy metal polluted canal.
abstract: Some investigations were made on the Satha canal water and health of dwelling fish Channa punctatus at Satha village, district Aligarh (U.P). Metal bioaccumulation and induction of biomarkers such as lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione S transferase (GST), reduced glutathione (GSH), DNA damage and histopathology are potential indicators of stress in C. punctatus exposed to effluents. In canal water Cr, Mn, Fe and Ni concentrations were exceeding the permissible limits set by both Bureau of Indian standards (BIS) and WHO. Fe (74%) was highly bioavailable and accumulated in all organs (gill, liver, kidney, muscle and integument). The highest metal pollution index (MPI) value of 53 was observed in gills and the lowest 6 in liver tissue. SOD and LPO were significantly higher in all tissues, whereas CAT, GST and GSH levels declined significantly compared to fish from the reference site. Concomitant damage to DNA was observed with significantly higher mean tail length in the exposed fish gill cells (19 μm) and in liver (12.7 μm) compared to reference fish (5 and 4 μm respectively). Histopathology in gill and liver also show significant damage. Therefore, it can be concluded that the sugar mill effluent has the potential to cause oxidative stress, DNA damage and histopathology in C. punctatus. This canal is a prime source of water and fish food to the local residents of the area. Therefore, the consumers may suffer adverse health effects like that in indicator organism.
authors.affiliation: Aquatic Toxicology Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India. Electronic address: mehjabeenjaved200@gmail.com.
authors.forename: Mehjbeen
authors.initials: M
authors.lastname: Javed
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
authors.forename: Irshad
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: Aquatic Toxicology Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
authors.forename: Nazura
authors.initials: N
authors.lastname: Usmani
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
authors.forename: Masood
authors.initials: M
authors.lastname: Ahmad
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage; D054750:Ecotoxicology; D005399:Fishes / Q000235:genetics* / Q000378:metabolism; D005618:Fresh Water / Q000737:chemistry*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D019216:Metals, Heavy; D009153:Mutagens; D014874:Water Pollutants, Chemical; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: Bioaccumulation; Biomarker; Comet assay; Heavy metals; Histopathology; Oxidative stress