title: Biological analysis and imaging applications of CdSe/CdSxSe1-x/CdS core-shell magic-sized quantum dot.
abstract: Although colloidal magic-sized quantum dots present great promise for biological applications due to their high stability and strong luminescence, nanotoxicological analyses are scarcely reported and biomedical applications have not been demonstrated. This is the first report on biological effects of CdSe/CdSxSe1-x/CdS core-shell magic-sized quantum dot (CS-MSQD) with specific application in breast cancer cell detection. The 2-nm CS-MSQD presents a broad bandwidth emission from 450 to 750nm, low toxicity, non-immunogenicity and biocompatibility. The CS-MSQD was conjugated to a breast cancer-specific Fab antibody, and passively diffused into cells for in vitro detection of a breast cancer cell line, demonstrating to be an unprecedented tool for biomedical applications.
authors.affiliation: Laboratório de Novos Materiais Isolantes e Semicondutores (LNMIS), Physics Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. Electronic address: acalmeida@ufu.br.
authors.forename: Anielle Christine Almeida
authors.initials: AC
authors.lastname: Silva
authors.affiliation: Laboratório de Novos Materiais Isolantes e Semicondutores (LNMIS), Physics Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil; Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Ana Paula Peres
authors.initials: AP
authors.lastname: Freschi
authors.affiliation: Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Cláudia Mendonça
authors.initials: CM
authors.lastname: Rodrigues
authors.affiliation: Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Bruna França
authors.initials: BF
authors.lastname: Matias
authors.affiliation: Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: Larissa Prado
authors.initials: LP
authors.lastname: Maia
authors.affiliation: Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil; Department of Medical Microbiology and Immunology, University of California, Davis, Davis, CA, USA.
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Goulart
authors.affiliation: Laboratório de Novos Materiais Isolantes e Semicondutores (LNMIS), Physics Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. Electronic address: noelio@ufu.br.
authors.forename: Noelio Oliveira
authors.initials: NO
authors.lastname: Dantas
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis*; D019187:Cadmium Compounds; D005260:Female; D006801:Humans; D049449:Luminescence; D049408:Luminescent Agents; D036103:Nanotechnology / Q000379:methods; D045663:Quantum Dots
chemical_list: D019187:Cadmium Compounds; D049408:Luminescent Agents
keywords: Biocompatible; Bioconjugation; Biomedical imaging; Breast cancer; CdSe/CdS(x)Se(1−x)/CdS CS-MSQDs