title: Concordance of folate receptor-α expression between biopsy, primary tumor and metastasis in breast cancer and lung cancer patients.
abstract: Folate receptor alpha (FRα) is known to be upregulated in a variety of cancers, including non-small cell lung cancer (NSCLC) and breast cancer. To ensure reliable implementation of diagnostic- and therapeutic agents, concordance of FRα expression between biopsy, primary tumor and metastases is important. Using immunohistochemistry (Mab 26B3.F2) these concordances were investigated in 60 NSCLC and 40 breast cancer patients. False positivity of FRα expression on breast and lung cancer biopsies was limited to less than 5%. In NSCLC, FRα expression was shown in 21/34 adenocarcinomas and 4/26 squamous cell carcinomas (SCC). Concordance of FRα expression between biopsy and primary tumor was achieved in respectively 83% and 91% of adenocarcinomas and SCCs. Approximately 80% of all local and distant metastases of NSCLC patients showed concordant FRα expression as their corresponding primary tumor. In breast cancer, FRα positivity was shown in 12/40 biopsies, 20/40 lumpectomies and 6/20 LN metastases, with concordance of 68% between biopsy and primary tumor and 60% between primary tumor and LN metastases. In conclusion, this study shows high concordance rates of FRα expression between biopsies and metastases compared to primary NSCLC and breast cancers, underscoring the applicability of FRα-targeted agents in these patients.
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Leonora S F
authors.initials: LS
authors.lastname: Boogerd
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Martin C
authors.initials: MC
authors.lastname: Boonstra
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Ann-Jean
authors.initials: AJ
authors.lastname: Beck
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Ayoub
authors.initials: A
authors.lastname: Charehbili
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Charlotte E S
authors.initials: CE
authors.lastname: Hoogstins
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Hendrica A J M
authors.initials: HA
authors.lastname: Prevoo
authors.affiliation: Department of Thoracic Surgery, Hospital of The University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Sunil
authors.initials: S
authors.lastname: Singhal
authors.affiliation: Department of Chemistry and Center for Drug Discovery, Purdue University, West Lafayette, IN, USA.
authors.forename: Philip S
authors.initials: PS
authors.lastname: Low
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Cornelis J H
authors.initials: CJ
authors.lastname: van de Velde
authors.affiliation: Department of Surgery, Leiden Univeristy Medical Center, Leiden, The Netherlands.
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Vahrmeijer
mesh_terms: D001706:Biopsy; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D015331:Cohort Studies; D005260:Female; D058975:Folate Receptor 1 / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging
chemical_list: D058975:Folate Receptor 1
keywords: Pathology Section; biomarker; diagnosis; oncology; personalized medicine; targeting