title: [Effects of Plasmid Fibroblast Growth Factor-2 Magnetic Chitosan Gelatin Microspheres on Proliferation and Differentiation of Mesenchymal Stem Cells].
abstract: The purpose of this study is to investigate the effect of superparamagnetic chitosan FGF-2 gelatin microspheres (SPCFGM) on the proliferation and differentiation of mouse mesenchymal stem cells. The superparamagnetic iron oxide chitosan nanoparticles (SPIOCNs) were synthesized by means of chemical co-precipitation, combined with FGF-2. Then The SPCFGM and superparamagnetic chitosan gelatin microspheres (SPCGM) were prepared by means of crosslinking-emulsion. The properties of SPCFGM and SPIONs were measured by laser diffraction particle size analyser and transmisson electron microscopy. The SPCFGM were measured for drug loading capacity, encapsulation efficiency and release pharmaceutical properties in vitro. The C3H10 cells were grouped according to the different ingredients being added to the culture medium: SPCFGM group, SPCGM group and DMEM as control group. Cell apoptosis was analyzed by DAPI staining. The protein expression level of FGF-2 was determined by Western blot. The proliferation activity and cell cycle phase of C3H10 were examined by CCK8 and flow cytometry. The results demonstrated that both of the SPIOCNs and SPCFGM were exhibited structure of spherical crystallization with a diameter of (25 ± 9) nm and (140 ± 12) μm, respectively. There were no apoptosis cells in the three group cells. Both the protein expression level of FGF-2 and cell proliferation activity increased significantly in the SPCFGM group cells (P < 0.05). The SPCFGM is successfully constructed and it can controlled-release FGF-2, remained the biological activity of FGF-2, which can promote proliferation activity of C3H10 cells, and are non-toxic to the cell.
authors.forename: Xingpo
authors.initials: X
authors.lastname: Ding
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Cao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.forename: Tongchuan
authors.initials: T
authors.lastname: He
authors.forename: Cong
authors.initials: C
authors.lastname: Luo
authors.forename: Haibing
authors.initials: H
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Bi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005780:Gelatin; D058185:Magnetite Nanoparticles; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D051379:Mice; D008863:Microspheres; D010957:Plasmids
chemical_list: D058185:Magnetite Nanoparticles; D016222:Fibroblast Growth Factor 2; D005780:Gelatin; D048271:Chitosan