title: Identification of chemical constituents in traditional Chinese medicine formula using HPLC coupled with linear ion trap-Orbitrap MS from high doses of medicinal materials to equivalent doses of formula: Study on Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules.
abstract: High-resolution mass spectrometry has been a powerful tool for the research of chemical constituents in traditional Chinese medicine (TCM) formulas. However, the chromatographic peaks were difficult to discriminate clearly in data collection or analysis because of the complexity and the greatly different content of the constituents in TCM formula, which increased the difficulty of identification. In this study, a high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry based strategy focused on the comprehensive identification of TCM formula constituents was developed. Identification was carried out from a high dose of medicinal materials to equivalent dose of formula. Meanwhile, combined with mass spectrometry data, chromatographic behaviors, reference standards and previous reports, the identification of constituents in Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules was described. 169 compounds were unambiguously or tentatively characterized, mainly including flavonoids, alkaloids, triterpenic acids, triterpene saponins, lactones, sesquiterpenoids and some other compounds. Among them, 11 compounds were unambiguously confirmed by comparing with reference standards. These results demonstrated that the method was effective and reliable for comprehensive identification of constituents of Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules extracts and reveal the material basis of its therapeutic effects. This strategy might propose a research idea for the characterization of multi-constituents in TCM formula.
authors.affiliation: Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, P.R. China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P.R. China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P.R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Lu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P.R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P.R. China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P.R. China.
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Fu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gao
authors.affiliation: Gastroenterology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, P.R. China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure
chemical_list: D004365:Drugs, Chinese Herbal; C000626071:xiang-sha-liu-jun-zi-jia-jian
keywords: Comprehensive identification; Extracted ion chromatograms; High-resolution mass spectrometry; Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules