title: Development of a combined pretreatment and hydrolysis strategy of rice straw for the production of bioethanol and biopolymer.
abstract: The present study highlights the development of a combined pretreatment and hydrolysis strategy of rice straw for the production of bioethanol and biopolymer (poly-3-hydroxybutyrate). Maximum reducing sugar yield was 0.374g/g. The hydrolyzate is devoid of major fermentation inhibitors like furfural and organic acids and can be used for fermentation without any detoxification. Fermentation of the non-detoxified hydrolyzate with Saccharomyces cerevisiae yielded 1.48% of ethanol with a fermentation efficiency of 61.25% and with Comamonas sp. yielded 35.86% of poly-3-hydroxybutyrate without any nutrient supplementation. Characterization of native, control as well as the residue left out after combined pretreatment and hydrolysis of RS by scanning electron microscopy and X-ray diffraction showed difference. Compositional analysis revealed that the residue contains lignin and hemicellulose as the major component indicating that major portion of cellulose were hydrolyzed in this strategy.
authors.affiliation: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India. Electronic address: sindhurgcb@gmail.com.
authors.forename: Raveendran
authors.initials: R
authors.lastname: Sindhu
authors.affiliation: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors.forename: Mathiyazhakan
authors.initials: M
authors.lastname: Kuttiraja
authors.affiliation: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors.forename: Thunoli Payyanvalappil
authors.initials: TP
authors.lastname: Prabisha
authors.affiliation: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors.forename: Parameswaran
authors.initials: P
authors.lastname: Binod
authors.affiliation: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Sukumaran
authors.affiliation: Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695 019, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
mesh_terms: D056804:Biofuels; D002482:Cellulose / Q000378:metabolism; D020585:Comamonas / Q000378:metabolism; D000431:Ethanol / Q000032:analysis* / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D006885:Hydroxybutyrates / Q000032:analysis* / Q000378:metabolism; D008031:Lignin / Q000032:analysis / Q000378:metabolism; D012275:Oryza / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D011091:Polyesters / Q000032:analysis* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014866:Waste Products; D014961:X-Ray Diffraction; D000094122:Polyhydroxybutyrates
chemical_list: D056804:Biofuels; D002482:Cellulose; D000431:Ethanol; D006885:Hydroxybutyrates; D008031:Lignin; D011091:Polyesters; D014866:Waste Products; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Combined; Hydrolysis; Lignocellulose; Pretreatment; Rice straw