title: Evaluation of Computed Tomography Perfusion Imaging in a Rat Acute Cerebral Ischemia-Reperfusion Model.
abstract: OBJECTIVE
  This study aims to apply computed tomography perfusion imaging (CTPI) technology under in vivo conditions in order to explore its reliability and accuracy in evaluating the rat acute cerebral ischemia-reperfusion model (RACIRM).
  
  
  METHODS
  The thread embolism method was used in 48 rats to create the RACIRM. Rats were divided into 2 groups as ischemia group and ischemia-reperfusion group. We then compared and evaluated the results of CTPI, 2,3,5-triphenyl tetrazolium chloride (TTC) staining, and hematoxylin and eosin (HE) staining of both groups.
  
  
  RESULTS
  There were no significant differences in the volumes of hypoperfusion regions in the cerebral blood flow (CBF) and cerebral blood volume (CBV) of each group at each CTPI time point and these volumes were not significantly different from the corresponding findings on the TTC-stained infarct regions. The mean transit time (MTT) did show a significant difference, as did the volumes observed in both the MTT ischemic region and TTC-stained infarct region. The CTPI parameters exhibited correlation with the infarct volumes calculated in TTC staining, among which CBV exhibited the highest correlation.
  
  
  CONCLUSIONS
  CTPI could rapidly, accurately, and non-invasively evaluate the site, size, and hemodynamic changes in the cerebral ischemia-reperfusion animal model.
authors.affiliation: Zhengzhou People's Hospital, Department of Radiology, Zhengzhou, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Ma
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Xiao
authors.forename: Mingquan
authors.initials: M
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Xue
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Jigang
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000502:physiology; D004195:Disease Models, Animal; D006439:Hemodynamics / Q000502:physiology; D008297:Male; D055420:Perfusion Imaging / Q000379:methods*; D011859:Radiography; D051381:Rats; D015427:Reperfusion Injury / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D015203:Reproducibility of Results