title: Evaluating Permeability Surface-Area Product as a Measure of Blood-Brain Barrier Permeability in a Murine Model.
abstract: Permeability surface-area product has been suggested as a marker for BBB permeability with potential applications in clinical care and research. However, few studies have demonstrated its correlation with actual quantitative measurements of BBB permeability. Our aim was to demonstrate the correlation of quantitative permeability surface-area product and BBB permeability in a murine model by histologic confirmation.
  
  
  
  Coronal MR imaging was performed on mice treated with mannitol (n = 6) for disruption of the BBB and controls treated with saline (n = 5). Permeability surface-area product was determined by ROI placement and was compared between saline- and mannitol-treated mice. Correlation was made with contrast-enhancement measurements and immunohistologic-stained sections of tripeptidyl peptidase-1 distribution in mice treated with mannitol and saline followed by injection of a viral vector containing the CLN2 gene, which directs production of tripeptidyl peptidase-1.
  
  
  
  Significantly increased permeability surface-area product was seen in mannitol- compared with saline-treated mice in the whole brain (P = .008), MCA territory (P = .014), and mixed vascular territories (P = .008). These findings were compared with contrast-enhancement measurements of BBB permeability and were correlated with immunohistologic-stained sections demonstrating BBB permeability to a large vector.
  
  
  
  Permeability surface-area product is increased in situations with known disruptions of the BBB, as evidenced by immunologic staining of large-vector passage through the BBB and concordance with contrast-enhancement measurements in a murine model. Quantitative permeability surface-area product has potential as an imaging marker of BBB permeability.
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: E K
authors.identifier: 0000-0002-3645-7964
authors.initials: EK
authors.lastname: Weidman
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: C P
authors.identifier: 0000-0001-8368-5671
authors.initials: CP
authors.lastname: Foley
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: O
authors.identifier: 0000-0002-2622-7479
authors.initials: O
authors.lastname: Kallas
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: J P
authors.identifier: 0000-0001-7170-488X
authors.initials: JP
authors.lastname: Dyke
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: A
authors.identifier: 0000-0003-2816-0340
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Division of Biostatistics and Epidemiology Department of Healthcare Policy and Research (A.E.G.), Weill Cornell Medical College, New York, New York.
authors.forename: A E
authors.identifier: 0000-0003-4498-8471
authors.initials: AE
authors.lastname: Giambrone
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: J
authors.identifier: 0000-0002-6470-5400
authors.initials: J
authors.lastname: Ivanidze
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: H
authors.identifier: 0000-0002-6251-5769
authors.initials: H
authors.lastname: Baradaran
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York.
authors.forename: D J
authors.identifier: 0000-0002-7162-9020
authors.initials: DJ
authors.lastname: Ballon
authors.affiliation: From the Department of Radiology (E.K.W., C.P.F., O.K., J.P.D., A.G., J.I., H.B., D.J.B., P.C.S.), New York-Presbyterian Hospital, Weill Cornell Medical College, New York, New York Department of Radiology (P.C.S.), Northwell Health, Manhasset, New York. psanelli@nshs.edu.
authors.forename: P C
authors.identifier: 0000-0002-9633-3699
authors.initials: PC
authors.lastname: Sanelli
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging* / Q000502:physiology; D002199:Capillary Permeability / Q000502:physiology*; D004195:Disease Models, Animal; D051379:Mice; D000091346:Tripeptidyl-Peptidase 1