title: Identification and Analysis of the Porcine MicroRNA in Porcine Cytomegalovirus-Infected Macrophages Using Deep Sequencing.
abstract: Porcine cytomegalovirus (PCMV; genus Cytomegalovirus, subfamily Betaherpesvirinae, family Herpesviridae) is an immunosuppressive virus that mainly inhibits the immune function of T lymphocytes and macrophages, which has caused substantial damage in the farming industry. In this study, we obtained the miRNA expression profiles of PCMV-infected porcine macrophages via high-throughput sequencing. The comprehensive analysis of miRNA profiles showed that 239 miRNA database-annotated and 355 novel pig-encoded miRNAs were detected. Of these, 130 miRNAs showed significant differential expression between the PCMV-infected and uninfected porcine macrophages. The 10 differentially expressed pig-encoded miRNAs were further determined by stem-loop reverse-transcription polymerase chain reaction, and the results were consistent with the high-throughput sequencing. Gene Ontology analysis of the target genes of miRNAs in PCMV-infected porcine macrophages showed that the differentially expressed miRNAs are mainly involved in immune and metabolic processes. This is the first report of the miRNA transcriptome in porcine macrophages and an analysis of the miRNA regulatory mechanisms during PCMV infection. Further research into the regulatory mechanisms of miRNAs during immunosuppressive viral infections should contribute to the treatment and prevention of immunosuppressive viruses.
authors.affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, China.|Liver Center and Gastrointestinal Division, Department of Medicine, Massachusetts, General Hospital, Harvard Medical School, Boston, MA, 02114, United States of America.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Liao
authors.affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, China.|Key Laboratory of Animal Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Animal Biotechnology Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya' an, 625014, China.
authors.forename: Wanzhu
authors.initials: W
authors.lastname: Guo
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000235:genetics* / Q000821:virology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008264:Macrophages / Q000378:metabolism / Q000821:virology*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D060888:Real-Time Polymerase Chain Reaction; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D035683:MicroRNAs