title: Data in support of the identification of neuronal and astrocyte proteins interacting with extracellularly applied oligomeric and fibrillar α-synuclein assemblies by mass spectrometry.
abstract: α-Synuclein (α-syn) is the principal component of Lewy bodies, the pathophysiological hallmark of individuals affected by Parkinson disease (PD). This neuropathologic form of α-syn contributes to PD progression and propagation of α-syn assemblies between neurons. The data we present here support the proteomic analysis used to identify neuronal proteins that specifically interact with extracellularly applied oligomeric or fibrillar α-syn assemblies (conditions 1 and 2, respectively) (doi: 10.15252/embj.201591397[1]). α-syn assemblies and their cellular partner proteins were pulled down from neuronal cell lysed shortly after exposure to exogenous α-syn assemblies and the associated proteins were identified by mass spectrometry using a shotgun proteomic-based approach. We also performed experiments on pure cultures of astrocytes to identify astrocyte-specific proteins interacting with oligomeric or fibrillar α-syn (conditions 3 and 4, respectively). For each condition, proteins interacting selectively with α-syn assemblies were identified by comparison to proteins pulled-down from untreated cells used as controls. The mass spectrometry data, the database search and the peak lists have been deposited to the ProteomeXchange Consortium database via the PRIDE partner repository with the dataset identifiers PRIDE: PXD002256 to PRIDE: PXD002263 and doi: 10.6019/PXD002256 to 10.6019/PXD002263.
authors.affiliation: École Normale Supérieure, Institut de Biologie de l'ENS (IBENS), INSERM, CNRS, PSL Research University, 46 Rue d׳Ulm, Paris 75005, France.
authors.forename: Amulya Nidhi
authors.initials: AN
authors.lastname: Shrivastava
authors.affiliation: Paris-Saclay Institute of Neuroscience, CNRS, Gif-sur-Yvette 91198, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Redeker
authors.affiliation: Department of Women and Children׳s Health, Karolinska Institutet, 171 76 Stockholm, Sweden.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fritz
authors.affiliation: Paris-Saclay Institute of Neuroscience, CNRS, Gif-sur-Yvette 91198, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Pieri
authors.affiliation: École Normale Supérieure, Institut de Biologie de l'ENS (IBENS), INSERM, CNRS, PSL Research University, 46 Rue d׳Ulm, Paris 75005, France.
authors.forename: Leandro G
authors.initials: LG
authors.lastname: Almeida
authors.affiliation: École Normale Supérieure, Institut de Biologie de l'ENS (IBENS), INSERM, CNRS, PSL Research University, 46 Rue d׳Ulm, Paris 75005, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Spolidoro
authors.affiliation: Department of Women and Children׳s Health, Karolinska Institutet, 171 76 Stockholm, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Liebmann
authors.affiliation: Paris-Saclay Institute of Neuroscience, CNRS, Gif-sur-Yvette 91198, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Bousset
authors.affiliation: École Normale Supérieure, Institut de Biologie de l'ENS (IBENS), INSERM, CNRS, PSL Research University, 46 Rue d׳Ulm, Paris 75005, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Renner
authors.affiliation: École Normale Supérieure, Institut de Biologie de l'ENS (IBENS), INSERM, CNRS, PSL Research University, 46 Rue d׳Ulm, Paris 75005, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Léna
authors.affiliation: Department of Women and Children׳s Health, Karolinska Institutet, 171 76 Stockholm, Sweden.
authors.forename: Anita
authors.initials: A
authors.lastname: Aperia
authors.affiliation: Paris-Saclay Institute of Neuroscience, CNRS, Gif-sur-Yvette 91198, France.
authors.forename: Ronald
authors.initials: R
authors.lastname: Melki
authors.affiliation: École Normale Supérieure, Institut de Biologie de l'ENS (IBENS), INSERM, CNRS, PSL Research University, 46 Rue d׳Ulm, Paris 75005, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Triller
keywords: Alpha-synuclein assemblies; Parkinson׳s disease; Proteomic Analysis; Pull down