title: Simultaneous monitoring of quantum dots and their assembly and disassembly with PreScission protease using capillary electrophoresis with fluorescence detection.
abstract: A novel assay was developed for the simultaneous monitoring of quantum dots and their assembly and disassembly with PreScission protease using capillary electrophoresis with fluorescence detection. Quantum dots and PreScission protease were injected into a capillary sequentially, then mixed and assembled via a thioether bond upon coupling to glutathione S-transferase tag inside the capillary. The in-capillary assembly was influenced by the molar ratio and the time interval of injection. Furthermore, the simultaneous monitoring of quantum dots and their assembly with PreScission protease and glutathione induced disassembly was achieved by adjusting the sampling sequence and the time interval of injection. More importantly, the in-capillary assay could be also applied to the online detection of glutathione.
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jinchen
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Pharmaceutical and Environmental Engineering, Changzhou Vocational Institute of Engineering, Changzhou, Jiangsu, P. R. China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Qin
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Ding
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Minli
authors.initials: M
authors.lastname: Fu
authors.affiliation: Changzhou Meisheng Biomaterials Co., Ltd, Changzhou, Jiangsu, P. R. China.
authors.forename: Junling
authors.initials: J
authors.lastname: Ji
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.
authors.forename: Pengju
authors.initials: P
authors.lastname: Jiang
authors.affiliation: School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, Jiangsu, P. R. China.|Changzhou Meisheng Biomaterials Co., Ltd, Changzhou, Jiangsu, P. R. China.|State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, Jiangsu, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
mesh_terms: D019075:Electrophoresis, Capillary; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism; D045663:Quantum Dots
chemical_list: D005982:Glutathione Transferase; D010447:Peptide Hydrolases
keywords: Capillary electrophoresis; Glutathione; Glutathione S-transferase tag; Quantum dots