title: Evaluation of the Accuracy of T-SPOT.TB for the Diagnosis of Ocular Tuberculosis in a BCG-vaccinated, Non-endemic Population.
abstract: OBJECTIVE
  To determine the performance of T-SPOT.TB, an interferon gamma release assay test, in patients with ocular tuberculosis (TB) in a BCG-vaccinated, non-endemic population.
  
  
  METHODS
  We employed a nested case-control design. In total, 45 subjects were enrolled (23 patients with ocular tuberculosis and 22 patients with other causes of uveitis). A blood sample was collected from each subject, and T-SPOT.TB was executed. Laboratory professionals were blinded to the disease status of each subject.
  
  
  RESULTS
  Five patients were excluded because of indeterminate results. The calculated sensitivity and specificity were 0.80 and 0.85, respectively. The positive likelihood ratio was 5.33 and the negative likelihood ratio was 0.23. The overall accuracy of the test was 0.83.
  
  
  CONCLUSIONS
  T-SPOT.TB adequately diagnosed ocular TB. This technique is particularly useful in populations where BCG vaccinations are still mandatory.
authors.affiliation: a Uveitis Department , Hospital del Salvador , Santiago , Chile.|b Ophthalmology Department , Universidad de Chile , Santiago , Chile.|c Hospital Clínico Universidad de Chile , Santiago , Chile.
authors.forename: Cristhian A
authors.initials: CA
authors.lastname: Urzua
authors.affiliation: d Ophthalmology Department , Pontificia Universidad Católica de Chile , Santiago , Chile.
authors.forename: Paulina
authors.initials: P
authors.lastname: Liberman
authors.affiliation: b Ophthalmology Department , Universidad de Chile , Santiago , Chile.|c Hospital Clínico Universidad de Chile , Santiago , Chile.
authors.forename: Sergio
authors.initials: S
authors.lastname: Abuauad
authors.affiliation: a Uveitis Department , Hospital del Salvador , Santiago , Chile.|b Ophthalmology Department , Universidad de Chile , Santiago , Chile.
authors.forename: Pablo
authors.initials: P
authors.lastname: Sabat
authors.affiliation: b Ophthalmology Department , Universidad de Chile , Santiago , Chile.|c Hospital Clínico Universidad de Chile , Santiago , Chile.
authors.forename: Enzo
authors.initials: E
authors.lastname: Castiglione
authors.affiliation: e Laboratory of Immunology , Hospital Clínico Universidad de Chile , Santiago , Chile.
authors.forename: María A
authors.initials: MA
authors.lastname: Beltran-Videla
authors.affiliation: e Laboratory of Immunology , Hospital Clínico Universidad de Chile , Santiago , Chile.
authors.forename: Raquel
authors.initials: R
authors.lastname: Aguilera
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D001500:BCG Vaccine / Q000008:administration & dosage*; D016022:Case-Control Studies; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D059425:Interferon-gamma Release Tests / Q000592:standards*; D016013:Likelihood Functions; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology; D014392:Tuberculosis, Ocular / Q000175:diagnosis* / Q000517:prevention & control; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D007371:Interferon-gamma
keywords: IGRA; T-SPOT.TB; non-endemic; ocular tuberculosis; sensitivity; specificity