title: A novel short anionic antibacterial peptide isolated from the skin of Xenopus laevis with broad antibacterial activity and inhibitory activity against breast cancer cell.
abstract: A vastarray of bioactive peptides from amphibian skin secretions is attracting increasing attention due to the growing problem of bacteria resistant to conventional antibiotics. In this report, a small molecular antibacterial peptide, named Xenopus laevis antibacterial peptide-P1 (XLAsp-P1), was isolated from the skin of Xenopus laevis using reversed-phase high-performance liquid chromatography. The primary structure of XLAsp-P1, which has been proved to be a novel peptide by BLAST search in AMP database, was DEDDD with a molecular weight of 607.7 Da analysed by Edman degradation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). The highlight of XLAsp-P1 is the strong in vitro potency against a variety of Gram-positive and Gram-negative bacteria with minimum inhibitory concentrations (MICs) starting at 10 μg/mL and potent inhibitory activity against breast cancer cell at tested concentrations from 5 to 50 μg/mL. In addition, only 6.2 % of red blood cells was haemolytic when incubated with 64 μg/mL (higher than MICs of all bacterial strain) of XLAsp-P1. The antimicrobial mechanism for this novel peptide was the destruction of the cell membrane investigated by transmission electron microscopy. All these showed that XLAsp-P1 is a novel short anionic antibacterial peptide with broad antibacterial activity and inhibitory activity against breast cancer cell.
authors.affiliation: College of Animal Science, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Hao
authors.affiliation: The Department of Infectious Disease of 1st Hospital, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Wanguo
authors.initials: W
authors.lastname: Bao
authors.affiliation: The Department of Infectious Disease of 1st Hospital, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.affiliation: College of Animal Science, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: College of Animal Science, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Nie
authors.affiliation: College of Animal Science, Jilin University, Changchun, 130062, Jilin Province, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Animal Science, Jilin University, Changchun, 130062, Jilin Province, China. houfeng999@hotmail.com.|IACA Biological Technology Co., Ltd., Tianjin, 300475, China. houfeng999@hotmail.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Hou
authors.affiliation: Institute of Translational Medicine, The First Hospital, Jilin University, Changchun, 130001, Jilin Province, China. yy86825@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D012867:Skin / Q000737:chemistry*; D014982:Xenopus laevis
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides
keywords: Antibacterial peptide; Breast cancer; Cell haemolysis; Short peptide