title: In vitro and In vivo Antioxidant Evaluation and Estimation of Total Phenolic, Flavonoidal Content of Mimosa pudica L.
abstract: OBJECTIVE
  Mimosa pudica Linn. (Mimosaceae) is traditionally used as a folk medicine to treat various ailments including convulsions, alopecia, diarrhea, dysentery, insomnia, tumor, wound healing, snake bite, etc., Here, the study was aimed to evaluate the antioxidant potential of M. pudica leaves extract against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) (in vitro) and its modulatory effect on rat brain enzymes.
  
  
  METHODS
  Total phenolic, flavonoid contents, and in vitro antioxidant potential against DPPH radical were evaluated from various extracts of M. pudica leaves. In addition, ethyl acetate extract of Mimosa pudica leaves (EAMP) in doses of 100, 200, and 400 mg/kg/day were administered orally for 7 consecutive days to albino rats and evaluated for the oxidative stress markers as thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) from rat brain homogenate.
  
  
  RESULTS
  The ethyl acetate extract showed the highest total phenolic content and total flavonoid content among other extracts of M. pudica leaves. The percentage inhibition and IC50 value of all the extracts were followed dose-dependency and found significant (P < 0.01) as compared to standard (ascorbic acid). The oxidative stress markers as SOD, CAT, and GSH were increased significantly (P < 0.01) at 200 and 400 mg/kg of EAMP treated animals and decreased significantly the TBARS level at 400 mg/kg of EAMP as compared to control group.
  
  
  CONCLUSIONS
  These results revealed that the ethyl acetate extract of M. pudica exhibits both in vitro antioxidant activity against DPPH and in vivo antioxidant activity by modulating brain enzymes in the rat. This could be further correlated with its potential to neuroprotective activity due to the presence of flavonoids and phenolic contents in the extract.
  
  
  CONCLUSIONS
  Total phenolic, flavonoid contents and in-vitro antioxidant potential were evaluated from various extracts of M. pudica leaves. Again, in-vivo antioxidant evaluation from brain homogenate on oxidative stress markers as TBARS, SOD, CAT and GSH from rat was investigated. Our findings revealed that M. pudica possesses both in-vitro and in-vivo antioxidant activity due to presence of phenolics and flavonoids.
authors.affiliation: School of Pharmaceutical Education and Research, Berhampur University, Bhanja, Bihar, Berhampur, Odisha, India.
authors.forename: Ganesh
authors.initials: G
authors.lastname: Patro
authors.affiliation: Department of Pharmacology, Roland Institute of Pharmaceutical Sciences, Berhampur, Odisha, India; Department of Life Sciences, International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia.
authors.forename: Subrat Kumar
authors.initials: SK
authors.lastname: Bhattamisra
authors.affiliation: Department of Botany and Biotechnology, Khallikote Autonomous College, Berhampur, Odisha, India.
authors.forename: Bijay Kumar
authors.initials: BK
authors.lastname: Mohanty
authors.affiliation: Department of Pharmacology and Experimental Biology, Vedica College of Pharmacy, RKDF University, Bhopal, Madhya Pradesh, India.
authors.forename: Himanshu Bhusan
authors.initials: HB
authors.lastname: Sahoo
keywords: 2; 2-diphenyl-1-picrylhydrazyl; Brain homogenate; Flavonoids; Mimosa pudica; Oxidative stress