title: Characterization of Chicken MMP13 Expression and Genetic Effect on Egg Production Traits of Its Promoter Polymorphisms.
abstract: Extracelluar matrix undergoes constant remodeling, cell-cell, and cell-matrix interactions during chicken ovarian follicle growth, which is coordinated by matrix metalloproteinases (MMPs), and their associated endogenous inhibitors (TIMPs). Transcriptome analysis revealed upregulation of MMP13 in sexually mature chicken ovaries. In this study, we found that the expression of MMP13 in chicken ovary was stably elevated from 60 d to 159 d, and was significantly higher at 159 d than at the other three developmental stages (P < 0.05). The expression of MMP13 mRNA increased from SW (small white follicles) to F5 (fifth largest follicles), then decreased to F1 (first largest follicles), and dramatically increased again in POF1 (newly postovulatory follicles) follicles (P < 0.05). The MMP13 protein was localized in stroma cells and primordial follicles of sexually immature chicken ovaries, in the theca cell layers of all sized follicles of sexually mature chicken ovaries. Furthermore, we identified a positive element (positions -1863 to -1036) controlling chicken MMP13 transcription, and, in this region, six single nucleotide polymorphisms were found and genotyped in chicken populations. In the White Recessive Rock population, hens with A(-1356)-C(-1079)/A(-1356)-C(-1079) genotype had earlier "age at first laying" than those with G(-1356)-T(-1079)/G(-1356)-T(-1079) genotype (P < 0.05), and exhibited significantly lower transcriptional activity (P < 0.01). Collectively, chicken MMP13 plays an important role in ovarian follicle growth and regression, and polymorphisms in its promoter region could be used as molecular markers for improving the trait "age at first laying" in chicken breeding.
authors.affiliation: Department of Genetics, College of Agronomy, Shandong Agricultural University, Taian 271018, China.
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors.forename: Li
authors.initials: L
authors.lastname: Kang
authors.affiliation: Department of Biology Science and Technology, Taishan University, Taian 271000, China.
authors.forename: Guiyu
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China yljiang723@aliyun.com.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D004531:Eggs; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D015810:Linkage Disequilibrium; D053509:Matrix Metalloproteinase 13 / Q000235:genetics* / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D019655:Quantitative Trait, Heritable; D012098:Reproduction / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D053509:Matrix Metalloproteinase 13
keywords: MMP13; age at first laying; chicken; follicle; polymorphism