title: Effect of Surface Functionalization on the Cellular Uptake and Toxicity of Nanozeolite A.
abstract: Extensive use of zeolite nanoparticles in many areas, including medicine, has led to the concern about an impact and possible risk of their use for human health and the environment.In our studies, we investigated an uptake, retention, and cytotoxicity of nanozeolite A (BaA) functionalized with aminopropyl or poly(ethylene glycol) (PEG) of different chain lengths using human cervical carcinoma cell line. For internalization studies, nanozeolite was labeled with (133)Ba radionuclide.The results show that in the case of PEG modification, toxicity and uptake depend on the PEG chain length. The highest toxicity has been observed for nanozeolites coated with short-length chain (Ba-silane-PEGm(MW350). Also, amine-modified nanozeolites exhibited high toxicity, while nanozeolites coated with long PEG molecules, BaA-silane-PEGm(MW1000), and BaA-silane-PEGm(MW2000), as well as unmodified nanozeolite, seem to be nontoxic.In conclusion, this study shows that uptake, retention, and toxicity of nanozeolites coated with various length PEG molecules groups depend on the molecular weight of PEG.
authors.affiliation: Institute of Nuclear Chemistry and Technology, Dorodna 16, Warsaw, 03-195, Poland. s.meczynska@ichtj.waw.pl.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Męczyńska-Wielgosz
authors.affiliation: Institute of Nuclear Chemistry and Technology, Dorodna 16, Warsaw, 03-195, Poland. a.kasperek@ichtj.waw.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Piotrowska
authors.affiliation: Institute of Nuclear Chemistry and Technology, Dorodna 16, Warsaw, 03-195, Poland. a.majkowska@ichtj.waw.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Majkowska-Pilip
authors.affiliation: Institute of Nuclear Chemistry and Technology, Dorodna 16, Warsaw, 03-195, Poland. a.bilewicz@ichtj.waw.pl.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Bilewicz
authors.affiliation: Institute of Nuclear Chemistry and Technology, Dorodna 16, Warsaw, 03-195, Poland. m.kruszewski@ichtj.waw.pl.|Faculty of Medicine, University of Information Technology and Management in Rzeszów, ul. Sucharskiego 2, Rzeszów, 35-225, Poland. m.kruszewski@ichtj.waw.pl.|Department of Molecular Biology and Translational Research, Institute of Rural Health, Jaczewskiego 2, Lublin, 20-090, Poland. m.kruszewski@ichtj.waw.pl.
authors.forename: Marcin
authors.initials: M
authors.lastname: Kruszewski
keywords: Cytotoxicity; Metabolic activity; Nanozeolite; PEG functionalization