title: A novel method to quantify the activity of alcohol acetyltransferase Using a SnO2-based sensor of electronic nose.
abstract: Alcohol acetyltransferase (AATFase) extensively catalyzes the reactions of alcohols to acetic esters in microorganisms and plants. In this work, a novel method has been proposed to quantify the activity of AATFase using a SnO2-based sensor of electronic nose, which was determined on the basis of its higher sensitivity to the reducing alcohol than the oxidizing ester. The maximum value of the first-derivative of the signals from the SnO2-based sensor was therein found to be an eigenvalue of isoamyl alcohol concentration. Quadratic polynomial regression perfectly fitted the correlation between the eigenvalue and the isoamyl alcohol concentration. The method was used to determine the AATFase activity in this type of reaction by calculating the conversion rate of isoamyl alcohol. The proposed method has been successfully applied to determine the AATFase activity of a cider yeast strain. Compared with GC-MS, the method shows promises with ideal recovery and low cost.
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Hu
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Huxuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Niu
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: yuetl305@nwsuaf.edu.cn.
authors.forename: Tianli
authors.initials: T
authors.lastname: Yue
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis* / Q000592:standards; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D062609:Electronic Nose; D004951:Esterification; D004952:Esters; D000431:Ethanol; D008401:Gas Chromatography-Mass Spectrometry; D000439:Pentanols / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D004952:Esters; D000439:Pentanols; D011506:Proteins; D000431:Ethanol; C029683:isopentyl alcohol; C079238:alcohol O-acetyltransferase
keywords: Acetyltransferase activity; Electronic nose; Feature extraction; Gas sensor; Isoamyl alcohol