title: In vivo expression of genes in the entomopathogenic fungus Beauveria bassiana during infection of lepidopteran larvae.
abstract: The entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin is commercially available as a bio insecticide. The expression of three genes previously identified to have a role in pathogenicity in in vitro studies was validated in vivo in three lepidopteran insects infected with B. bassiana. Expression of all three genes was observed in all the tested insects starting from 48 or 72h to 10d post infection corroborating their role in pathogenicity. We suggest that it is essential to test the expression of putative pathogenicity genes both in vitro and in vivo to understand their role in different insect species.
authors.affiliation: Department of Botany, Andhra University, Visakhapatnam, 530003 AP, India.
authors.forename: Sandhya
authors.initials: S
authors.lastname: Galidevara
authors.affiliation: Hochschule Geisenheim University, Department of Phytomedicine, Von-Lade-Str. 1, D-65366 Geisenheim, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Reineke
authors.affiliation: Department of Botany, Andhra University, Visakhapatnam, 530003 AP, India. Electronic address: umadevikoduru@gmail.com.
authors.forename: Uma Devi
authors.initials: UD
authors.lastname: Koduru
mesh_terms: D000818:Animals; D052982:Beauveria / Q000235:genetics* / Q000472:pathogenicity*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D009036:Moths / Q000382:microbiology*; D009181:Mycoses / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
keywords: Beauveria bassiana; Entomopathogenic fungus; In vivo expression; Pathogenicity genes; qPCR