title: Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus.
abstract: Several studies have explored the origin and development mechanism of oral lichen planus (OLP) with limited attention to the role of bacteria in the progression of this common oral disease. Here we utilized MiSeq sequencing of 16S rRNA gene amplicons to identify complex oral microbiota associated with OLP from saliva samples of two subtypes (reticular and erosive) of OLP patients and healthy controls. Our analyses indicated that the overall structure of the salivary microbiome was not significantly affected by disease status. However, we did observe evident variations in abundance for several taxonomic groups in OLP. Porphyromonas and Solobacterium showed significantly higher relative abundances, whereas Haemophilus, Corynebacterium, Cellulosimicrobium and Campylobacter showed lower abundances in OLP patients, as compared with healthy controls. In addition, we explored specific microbial co-occurrence patterns in OLP, and revealed significantly fewer linkers of Streptococcus comprising species in erosive OLP. Furthermore, the disease severity and immune dysregulation were also genus-associated, including with Porphyromonas that correlated to disease scores and salivary levels of interleukin (IL)-17 and IL-23. Overall, this study provides a general description of oral microbiome in OLP, and it will be useful for further investigation of their potential roles in the initiation and immune modulation of OLP.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Marine Sciences, Zhejiang University, Zhejiang, China.|Institute for Environmental Genomics, Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA.
authors.forename: Qichao
authors.initials: Q
authors.lastname: Tu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Jinzhi
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Gou
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA.
authors.forename: Joy D
authors.initials: JD
authors.lastname: Van Nostrand
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Jiyao
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute for Environmental Genomics, Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA.
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000328:Adult; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D003352:Corynebacterium / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005260:Female; D006190:Haemophilus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D017676:Lichen Planus, Oral / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D008297:Male; D064307:Microbiota / Q000235:genetics*; D008875:Middle Aged; D017861:Porphyromonas / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012463:Saliva / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S