title: Leukocyte receptor expression in chronic periodontitis.
abstract: OBJECTIVE
  Microbial recognition in the periodontium through specific leukocyte receptors gives rise to the response which in susceptible individuals can lead to periodontal diseases. The aim of this study was to explore the expression of leukocyte receptors in the gingival tissues of chronic periodontitis patients and to analyse differences between diseased and control sites (sites with probing pocket depth <4 mm).
  
  
  METHODS
  Thirty-seven chronic periodontitis patients were included in the study. Gingival biopsies were harvested from diseased and control sites and processed by flow cytometry for the determination of the expression of 16 leukocyte receptors (CD4, CD8, CD11b, CD14, CD16, CD19, CD25, CD28, CD49d, CD49e, CD62, CD71, CD80, CCR7, Ly6G and HLA-DR).
  
  
  RESULTS
  Expression of all studied receptors was higher in test compared with control sites (p < 0.005). Sampled sites with less bleeding on probing exhibited higher expression of CD16 and CD14 receptors (p = 0.020 and 0.011, respectively).
  
  
  CONCLUSIONS
  This study points towards considerable differences in the expression of leukocyte receptors between diseased and control sites in the same periodontal patients.
authors.affiliation: Clinical Oral Research Centre, Institute of Dentistry, Queen Mary University London (QMUL), London, UK.
authors.forename: L
authors.initials: L
authors.lastname: Nibali
authors.affiliation: Oral Sciences Research Group, Periodontology and Special Needs Units, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Novoa
authors.affiliation: Clinical Oral Research Centre, Institute of Dentistry, Queen Mary University London (QMUL), London, UK.
authors.forename: N
authors.initials: N
authors.lastname: Donos
authors.affiliation: Division of Microbial Diseases, UCL Eastman Dental Institute, London, UK.
authors.forename: B
authors.initials: B
authors.lastname: Henderson
authors.affiliation: Oral Sciences Research Group, Periodontology and Special Needs Units, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.affiliation: Oral Sciences Research Group, Periodontology and Special Needs Units, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Santiago de Compostela, Spain. inmaculada.tomas@usc.es.
authors.forename: I
authors.initials: I
authors.lastname: Tomas
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D055113:Chronic Periodontitis / Q000276:immunology* / Q000382:microbiology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology*
chemical_list: D016028:Receptors, Leukocyte-Adhesion
keywords: Bacteria; Leukocyte receptors; Periodontitis