title: Preparation and evaluation of a novel molecularly imprinted polymer coating for selective extraction of indomethacin from biological samples by electrochemically controlled in-tube solid phase microextraction.
abstract: In the present work, an automated on-line electrochemically controlled in-tube solid-phase microextraction (EC-in-tube SPME) coupled with HPLC-UV was developed for the selective extraction and preconcentration of indomethacin as a model analyte in biological samples. Applying an electrical potential can improve the extraction efficiency and provide more convenient manipulation of different properties of the extraction system including selectivity, clean-up, rate, and efficiency. For more enhancement of the selectivity and applicability of this method, a novel molecularly imprinted polymer coated tube was prepared and applied for extraction of indomethacin. For this purpose, nanostructured copolymer coating consisting of polypyrrole doped with ethylene glycol dimethacrylate was prepared on the inner surface of a stainless-steel tube by electrochemical synthesis. The characteristics and application of the tubes were investigated. Electron microscopy provided a cross linked porous surface and the average thickness of the MIP coating was 45 μm. Compared with the non-imprinted polymer coated tubes, the special selectivity for indomethacin was discovered with the molecularly imprinted coated tube. Moreover, stable and reproducible responses were obtained without being considerably influenced by interferences commonly existing in biological samples. Under the optimal conditions, the limits of detection were in the range of 0.07-2.0 μg L(-1) in different matrices. This method showed good linearity for indomethacin in the range of 0.1-200 μg L(-1), with coefficients of determination better than 0.996. The inter- and intra-assay precisions (RSD%, n = 3) were respectively in the range of 3.5-8.4% and 2.3-7.6% at three concentration levels of 7, 70 and 150 μg L(-1). The results showed that the proposed method can be successfully applied for selective analysis of indomethacin in biological samples.
authors.affiliation: Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Asiabi
authors.affiliation: Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran. Electronic address: yyamini@modares.ac.ir.
authors.forename: Yadollah
authors.initials: Y
authors.lastname: Yamini
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, K.N. Toosi University of Technology, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Seidi
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, K.N. Toosi University of Technology, Tehran, Iran.
authors.forename: Fazel
authors.initials: F
authors.lastname: Ghahramanifard
mesh_terms: D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques / Q000379:methods*; D007213:Indomethacin / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D052617:Solid Phase Microextraction / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007213:Indomethacin
keywords: Electrochemically controlled; In-tube solid phase microextraction; Indomethacin; Molecularly imprinted polymer; Polypyrrole