title: Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.
abstract: The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 °C at a cooling rate of 1 °C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 °C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 °C or in LN. This result indicated that testicular cells can be stored at -80 °C and/or on dry ice, for 2 days with minimum loss of viability.
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic. Electronic address: dehsari@frov.jcu.cz.
authors.forename: Amin
authors.initials: A
authors.lastname: Golpour
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
authors.forename: Mohammad Abdul Momin
authors.initials: MA
authors.lastname: Siddique
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Rodina
authors.affiliation: Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Pšenička
mesh_terms: D000818:Animals; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D056727:Endangered Species; D019855:Ethylene Glycol / Q000494:pharmacology; D005399:Fishes; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D013696:Temperature; D013737:Testis / Q000166:cytology*
chemical_list: D003451:Cryoprotective Agents; D019855:Ethylene Glycol
keywords: Aciperserids; Cryopreservation; Germ cells; Sterlet