title: Poly (γ-glutamic acid)/beta-TCP nanocomposites via in situ copolymerization: Preparation and characterization.
abstract: A series biodegradable poly (γ-glutamic acid)/beta-tricalcium phosphate (γ-PGA/TCP) nanocomposites were prepared which were composed of poly-γ-glutamic acid polymerized in situ with β-tricalcium phosphate and physiochemically characterized as bone graft substitutes. The particle size via dynamic light scattering, the direct morphological characterization via transmission electron microscopy and field emission scanning electron microscope, which showed that γ-PGA and β-TCP were combined compactly at 80℃, and the γ-PGA/TCP nanocomposites had homogenous and nano-sized grains with narrow particle size distributions. The water uptake and retention abilities, in vitro degradation properties, cytotoxicity in the simulated medium, and protein release of these novel γ-PGA/TCP composites were investigated. Cell proliferation in composites was nearly twice than β-TCP when checked in vitro using MC3T3 cell line. We also envision the potential use of γ-PGA/TCP systems in bone growth factor or orthopedic drug delivery applications in future bone tissue engineering applications. These observations suggest that the γ-PGA/TCP are novel nanocomposites with great potential for application in the field of bone tissue engineering.
authors.affiliation: Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China.
authors.forename: Xiu-Lin
authors.initials: XL
authors.lastname: Shu
authors.affiliation: Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China jigan@gdim.cn.
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Shi
authors.affiliation: Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Feng
authors.affiliation: Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China.
authors.forename: Yun-Hua
authors.initials: YH
authors.lastname: Yang
authors.affiliation: Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Guangdong Institute of Microbiology, China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, China State Key Laboratory of Applied Microbiology Southern China, China.
authors.forename: Wen-Ru
authors.initials: WR
authors.lastname: Li
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis*; D001842:Bone and Bones; D002130:Calcium Phosphates / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004058:Diffusion; D008422:Materials Testing; D051379:Mice; D053769:Nanocapsules / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010316:Particle Size; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D003692:Delayed-Action Preparations; D053769:Nanocapsules; D011108:Polymers; C485817:beta-tricalcium phosphate; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid
keywords: Nano composites; bioactivity; bone engineering; poly-γ-glutamic acid; β-tricalcium phosphate