title: Cyanobacteria Affect Fitness and Genetic Structure of Experimental Daphnia Populations.
abstract: Zooplankton communities can be strongly affected by cyanobacterial blooms, especially species of genus Daphnia, which are key-species in lake ecosystems. Here, we explored the effect of microcystin/nonmicrocystin (MC/non-MC) producing cyanobacteria in the diet of experimental Daphnia galeata populations composed of eight genotypes. We used D. galeata clones hatched from ephippia 10 to 60 years old, which were first tested in monocultures, and then exposed for 10 weeks as mixed populations to three food treatments consisting of green algae combined with cyanobacteria able/unable of producing MC. We measured the expression of nine genes potentially involved in Daphnia acclimation to cyanobacteria: six protease genes, one ubiquitin-conjugating enzyme gene, and two rRNA genes, and then we tracked the dynamics of the genotypes in mixed populations. The expression pattern of one protease and the ubiquitin-conjugating enzyme genes was positively correlated with the increased fitness of competing clones in the presence of cyanobacteria, suggesting physiological plasticity. The genotype dynamics in mixed populations was only partially related to the growth rates of clones in monocultures and varied strongly with the food. Our results revealed strong intraspecific differences in the tolerance of D. galeata clones to MC/non-MC-producing cyanobacteria in their diet, suggesting microevolutionary effects.
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology , Department of Aquatic Ecology, 133 Überlandstrasse, 8600 Dübendorf, Switzerland.|Institute of Biological Research , Branch of the National Institute of Research and Development for Biological Sciences, 48 Republicii, 400015 Cluj-Napoca, Romania.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Drugă
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology , Department of Aquatic Ecology, 133 Überlandstrasse, 8600 Dübendorf, Switzerland.|Institute of Integrative Biology, ETH Zurich , Universitätstrasse 16, 8092 Zürich, Switzerland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Turko
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology , Department of Aquatic Ecology, 133 Überlandstrasse, 8600 Dübendorf, Switzerland.|Institute of Integrative Biology, ETH Zurich , Universitätstrasse 16, 8092 Zürich, Switzerland.
authors.forename: Piet
authors.initials: P
authors.lastname: Spaak
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology , Department of Aquatic Ecology, 133 Überlandstrasse, 8600 Dübendorf, Switzerland.|Institute of Integrative Biology, ETH Zurich , Universitätstrasse 16, 8092 Zürich, Switzerland.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pomati
mesh_terms: D000064:Acclimatization; D000818:Animals; D000460:Chlorophyta; D000458:Cyanobacteria / Q000378:metabolism*; D003621:Daphnia / Q000235:genetics* / Q000502:physiology; D017753:Ecosystem; D020387:Food Chain; D020869:Gene Expression Profiling; D056084:Genetic Fitness; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D060106:Lakes; D052998:Microcystins / Q000378:metabolism; D018895:Microsatellite Repeats; D044382:Population Groups; D013557:Switzerland; D015048:Zooplankton
chemical_list: D052998:Microcystins; C078588:microcystin