title: The nucleic acid-binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii.
abstract: Gene family encoding cellular nucleic acid binding proteins (CNBP) is well conserved among vertebrates; however, there is limited knowledge in lower organisms. In this study, a CNBP homolog from the red swamp crayfish Procambarus clarkii was characterised. The full-length cDNA of PcCNBP was of 1257 bp with a 5'-untranslated region (UTR) of 63 bp and a 3'-UTR of 331 bp with a poly (A) tail, and an open-reading frame (ORF) of 864 bp encoding a polypeptide of 287 amino acids with the predicted molecular weight of about 33 kDa. The predicted protein possesses 7 tandem repeats of 14 amino acids containing the CCHC zinc finger consensus sequence, two RGG-rich single-stranded RNA-binding domain and a nuclear localization signal, strongly suggesting that PcCNBP was a homolog of vertebrate CNBP. The PcCNBP transcript was constitutively expressed in all tested tissues of unchallenged crayfish, including hepatopancreas, gill, eyestalk, haemocytes, intestine, stomach and cuticle with highest expression in haemocytes, intestine, gills and hepatopancreas. The mRNA expression of PcCNBP in haemocytes was modulated at transcriptional level by different immune challenges, suggesting its involvement in the immune response of P. clarkii during both bacteria and viruses infection.
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR) Detached Unit of Capo Granitola, Torretta Granitola, Trapani, Sicily, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Nicosia
authors.affiliation: Dipartimento Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, University of Palermo, Sicily, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Costa
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR) Detached Unit of Capo Granitola, Torretta Granitola, Trapani, Sicily, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Tagliavia
authors.affiliation: Institute for Environmental Protection and Research-ISPRA, Palermo, 90143, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Maggio
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR) Detached Unit of Capo Granitola, Torretta Granitola, Trapani, Sicily, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Salamone
authors.affiliation: Dipartimento Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, University of Palermo, Sicily, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Adamo
authors.affiliation: Dipartimento Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, University of Palermo, Sicily, Italy.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Ragusa
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR) Detached Unit of Capo Granitola, Torretta Granitola, Trapani, Sicily, Italy.
authors.forename: Carmelo
authors.initials: C
authors.lastname: Bennici
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR) Detached Unit of Capo Granitola, Torretta Granitola, Trapani, Sicily, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Masullo
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR) Detached Unit of Capo Granitola, Torretta Granitola, Trapani, Sicily, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mazzola
authors.affiliation: Dipartimento Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, University of Palermo, Sicily, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Gianguzza
authors.affiliation: Laboratory of Molecular Ecology and Biotechnology, National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR) Detached Unit of Capo Granitola, Torretta Granitola, Trapani, Sicily, Italy. angela.cuttitta@iamc.cnr.it.
authors.forename: Angela
authors.initials: A
authors.lastname: Cuttitta
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics*; D000818:Animals; D003400:Astacoidea / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006434:Hemocytes / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics; D058977:Molecular Sequence Annotation; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D016601:RNA-Binding Proteins
keywords: Crayfish; Gene expression pattern; Innate immunity; Nucleic acid-binding protein; Zinc finger motifs