title: Do We Pay Enough Attention to Culture Conditions in Context of Perinatal Outcome after In Vitro Fertilization? Up-to-Date Literature Review.
abstract: Adverse perinatal outcomes in singleton IVF pregnancies have been most often explained by parental underlying diseases and so far laboratory conditions during embryo culture are still not explored well. The following review discusses the current state of knowledge on the influence of IVF laboratory procedures on the possible perinatal outcome. The role of improved media for human embryo culture is unquestionable. Addition of certain components to culture media and their effect on embryo survival and implantation rates have been taken into consideration recently and studied on animal model. Impact of media on perinatal outcome in IVF offspring has also been studied. It has been discovered that epigenetic changes and neonatal birth weight are probably associated with the use of specific culture media, as is the relation between placental size and its influence on perinatal outcome. There are still questions in the discussion about duration of embryo culture (cleavage stage versus blastocyst transfer). Some of the IVF methods, such as in vitro maturation of oocytes and freezing/thawing procedures, also require well-powered randomized controlled trials in order to define their exact impact on perinatal outcome. Constant further research is needed to assess the impact of laboratory environment on fetal and postnatal development.
authors.affiliation: 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland.
authors.forename: Piotr
authors.identifier: 0000-0002-4518-2210
authors.initials: P
authors.lastname: Marianowski
authors.affiliation: 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland.
authors.forename: Filip A
authors.identifier: 0000-0002-8557-4091
authors.initials: FA
authors.lastname: Dąbrowski
authors.affiliation: 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland.
authors.forename: Aleksandra
authors.identifier: 0000-0001-6935-987X
authors.initials: A
authors.lastname: Zyguła
authors.affiliation: 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland.
authors.forename: Mirosław
authors.identifier: 0000-0003-2581-3668
authors.initials: M
authors.lastname: Wielgoś
authors.affiliation: 1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Starynkiewicza Square 1/3, 02-015 Warsaw, Poland.
authors.forename: Iwona
authors.identifier: 0000-0001-8106-5428
authors.initials: I
authors.lastname: Szymusik
mesh_terms: D001724:Birth Weight; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation / Q000187:drug effects; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000009:adverse effects*; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome
chemical_list: D003470:Culture Media