title: New Coll-HA/BT composite materials for hard tissue engineering.
abstract: The integration of ceramic powders in composite materials for bone scaffolds can improve the osseointegration process. This work was aimed to the synthesis and characterization of new collagen-hydroxyapatite/barium titanate (Coll-HA/BT) composite materials starting from barium titanate (BT) nanopowder, hydroxyapatite (HA) nanopowder and collagen (Coll) gel. BT nanopowder was produced by combining two wet-chemical approaches, sol-gel and hydrothermal methods. The resulting materials were characterized in terms of phase composition and microstructure by X-ray diffraction, Raman spectroscopy, scanning electron microscopy and transmission electron microscopy. Moreover, the biocompatibility and bioactivity of the composite materials were assessed by in vitro tests. The synthesized BT particles exhibit an average size of around 35 nm and a spherical morphology, with a pseudo-cubic or tetragonal symmetry. The diffraction spectra of Coll-HA and Coll-HA/BT composite materials indicate a pronounced interaction between Col and the mineral phases, meaning a good mineralization of Col fibres. As well, the in vitro tests highlight excellent osteoinductive properties for all biological samples, especially for Coll-HA/BT composite materials, fact that can be attributed to the ferromagnetic properties of BT.
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, "Politehnica" University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest, Romania.
authors.forename: Andrei Vlad
authors.initials: AV
authors.lastname: Zanfir
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, "Politehnica" University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest, Romania. Electronic address: getav2001@yahoo.co.uk.
authors.forename: Georgeta
authors.initials: G
authors.lastname: Voicu
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, "Politehnica" University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest, Romania.
authors.forename: Cristina
authors.initials: C
authors.lastname: Busuioc
authors.affiliation: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, "Politehnica" University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest, Romania.
authors.forename: Sorin Ion
authors.initials: SI
authors.lastname: Jinga
authors.affiliation: Department of Collagen, Branch of Leather and Footwear Research, National Institute of Research and Development for Textile and Leather, 93 I. Minulescu Street, RO-031215 Bucharest, Romania.
authors.forename: Madalina Georgiana
authors.initials: MG
authors.lastname: Albu
authors.affiliation: Department of Fetal and Adult Stem Cell Therapy, "Nicolae Simionescu" Institute of Cellular Biology and Pathology of Romanian Academy, 8 B.P. Hasdeu Street, RO-050568 Bucharest, Romania.
authors.forename: Florin
authors.initials: F
authors.lastname: Iordache
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000650:Amnion / Q000166:cytology / Q000378:metabolism; D017609:Barium Compounds / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D055395:Bone Morphogenetic Protein 1 / Q000235:genetics / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013059:Spectrum Analysis, Raman; D023822:Tissue Engineering; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D017609:Barium Compounds; D001672:Biocompatible Materials; D024042:Collagen Type I; D006882:Hydroxyapatites; D014867:Water; D053495:Osteopontin; C041297:barium hydroxyapatite; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1
keywords: Biomaterials; Collagen mineralization; Composite materials; Hard tissue scaffolds; Wet-chemical synthesis