title: A New Acyl-homoserine Lactone Molecule Generated by Nitrobacter winogradskyi.
abstract: It is crucial to reveal the regulatory mechanism of nitrification to understand nitrogen conversion in agricultural systems and wastewater treatment. In this study, the nwiI gene of Nitrobacter winogradskyi was confirmed to be a homoserine lactone synthase by heterologous expression in Escherichia coli that synthesized several acyl-homoserine lactone signals with 7 to 11 carbon acyl groups. A novel signal, 7, 8-trans-N-(decanoyl) homoserine lactone (C10:1-HSL), was identified in both N. winogradskyi and the recombined E. coli. Furthermore, this novel signal also triggered variances in the nitrification rate and the level of transcripts for the genes involved in the nitrification process. These results indicate that quorum sensing may have a potential role in regulating nitrogen metabolism.
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Qiuxuan
authors.initials: Q
authors.lastname: Shen
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shuangjiang
authors.initials: S
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Zijun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Yinghuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Baoyuan
authors.initials: B
authors.lastname: Guo
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
mesh_terms: D054742:Acyl-Butyrolactones / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009576:Nitrobacter / Q000235:genetics / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D053038:Quorum Sensing; D062065:Wastewater / Q000382:microbiology
chemical_list: D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D009584:Nitrogen; D062065:Wastewater