title: DNA Aptamers against Taiwan Banded Krait α-Bungarotoxin Recognize Taiwan Cobra Cardiotoxins.
abstract: Bungarus multicinctus α-bungarotoxin (α-Bgt) and Naja atra cardiotoxins (CTXs) share a common structural scaffold, and their tertiary structures adopt three-fingered loop motifs. Four DNA aptamers against α-Bgt have been reported previously. Given that the binding of aptamers with targeted proteins depends on structural complementarity, in this study, we investigated whether DNA aptamers against α-Bgt could also recognize CTXs. It was found that N. atra cardiotoxin 3 (CTX3) reduced the electrophoretic mobility of aptamers against α-Bgt. Analysis of the changes in the fluorescence intensity of carboxyfluorescein-labeled aptamers upon binding toxin molecules revealed that CTX3 and α-Bgt could bind the tested aptamers. Moreover, the aptamers inhibited the membrane-damaging activity and cytotoxicity of CTX3. In addition to CTX3, other N. atra CTX isotoxins also bound to the aptamer against α-Bgt. Taken together, our data indicate that aptamers against α-Bgt show cross-reactivity with CTXs. The findings that aptamers against α-Bgt also suppress the biological activities of CTX3 highlight the potential utility of aptamers in regard to the broad inhibition of snake venom three-fingered proteins.
authors.affiliation: Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. yjchen@mail.nsysu.edu.tw.
authors.forename: Ying-Jung
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. m012050015@student.nsysu.edu.tw.
authors.forename: Chia-Yu
authors.initials: CY
authors.lastname: Tsai
authors.affiliation: Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wphu@kmu.edu.tw.
authors.forename: Wan-Ping
authors.initials: WP
authors.lastname: Hu
authors.affiliation: Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan. lschang@mail.nsysu.edu.tw.|Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan. lschang@mail.nsysu.edu.tw.
authors.forename: Long-Sen
authors.initials: LS
authors.lastname: Chang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D002038:Bungarotoxins / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004179:Cobra Cardiotoxin Proteins / Q000378:metabolism* / Q000633:toxicity; D017815:Elapidae; D006801:Humans; D020014:K562 Cells; D011485:Protein Binding
chemical_list: D052157:Aptamers, Nucleotide; D002038:Bungarotoxins; D004179:Cobra Cardiotoxin Proteins; C082202:cardiotoxin III, Naja naja atra
keywords: aptamer; cardiotoxins; cytotoxicity; membrane-damaging activity; α-bungarotoxin