title: Determination of donepezil in serum samples using molecularly imprinted polymer nanoparticles followed by high-performance liquid chromatography with ultraviolet detection.
abstract: A molecularly imprinted polymer designed for the selective extraction of donepezil from serum samples was synthesized using a noncovalent molecular imprinting approach. The molecularly imprinted polymer was evaluated chromatographically and then its affinity for donepezil was confirmed by solid-phase extraction. The optimal conditions for solid-phase extraction were provided by cartridge conditioning using acidified water purified from a Milli-Q system, sample loading under basic aqueous conditions, clean-up using acetonitrile, and elution with methanol/tetrahydrofuran. Desirable molecular recognition properties of the molecularly imprinted polymer led to good donepezil recoveries (90-102%). The data indicated that the imprinted polymer has a perfect selectivity and affinity for donepezil and could be used for selective extraction and analysis of donepezil in human serum.
authors.affiliation: Research Center, School of Chemical Engineering, Iran University of Science and Technology, Tehran, Iran.|Research Center, Faculty of Pharmacy, Shaheed Beheshtee University of Medical Sciences, Tehran, Iran.
authors.forename: Mehdi Rajabnia
authors.initials: MR
authors.lastname: Khansari
authors.affiliation: Research Center, Faculty of Pharmacy, Shaheed Beheshtee University of Medical Sciences, Tehran, Iran.
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Bikloo
authors.affiliation: Research Center, School of Chemical Engineering, Iran University of Science and Technology, Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Shahreza
mesh_terms: D002800:Cholinesterase Inhibitors / Q000097:blood / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D000077265:Donepezil; D006801:Humans; D007189:Indans / Q000097:blood / Q000302:isolation & purification*; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry*; D010880:Piperidines / Q000097:blood / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D002800:Cholinesterase Inhibitors; D007189:Indans; D010880:Piperidines; D011108:Polymers; D000077265:Donepezil
keywords: Donepezil; Molecularly imprinted polymers; Serum samples; Solid-phase extraction