title: A novel collagen/platelet-rich plasma (COL/PRP) scaffold: preparation and growth factor release analysis.
abstract: Platelet-rich plasma (PRP) has been widely used in clinical practice for more than 20 years because it causes the release of many growth factors. However, the burst release pattern and short release period of PRP have become obstacles to its application. An optimal controllable release system is an urgent need for researchers. This study investigated whether collagen/PRP (COL/PRP) scaffolds can serve as a vehicle for the controllable release of growth factors. We fabricated a novel scaffold that integrates PRP activated by thrombin or collagen into type I collagen. The mechanical properties, cytotoxicity, and transforming growth factor β1 (TGF-β1), platelet derived growth factor (PDGF), fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) content were evaluated. Our results demonstrate that the COL/PRP scaffolds were not cytotoxic to L-929 fibroblasts. The PDGF and FGF content in the thrombin group was at a higher level and lasted for a long period of time. Collagen and thrombin played the same role in the release of TGF-β1 and VEGF. These data suggest that the novel COL/PRP scaffolds provide a carrier for the controllable release of growth factors and may be used in tissue- regenerative therapies.
authors.affiliation: GongRen Hospital, Tangshan, 063000, China.
authors.forename: Xiujie
authors.initials: X
authors.lastname: Zhang
authors.affiliation: GongRen Hospital, Tangshan, 063000, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Wang
authors.affiliation: GongRen Hospital, Tangshan, 063000, China.
authors.forename: Mingguang
authors.initials: M
authors.lastname: Ren
authors.affiliation: GongRen Hospital, Tangshan, 063000, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Li
authors.affiliation: GongRen Hospital, Tangshan, 063000, China.
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Wang
authors.affiliation: GongRen Hospital, Tangshan, 063000, China. 1093071950@qq.com.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Hou
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002498:Centrifugation; D003094:Collagen / Q000494:pharmacology* / Q000648:ultrastructure; D019245:Compressive Strength / Q000187:drug effects; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D013917:Thrombin / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D003094:Collagen; D013917:Thrombin
keywords: Activate; Growth factor; Platelet-rich plasma; Release; Scaffold