title: Design of Asymmetric Peptide Bilayer Membranes.
abstract: Energetic insights emerging from the structural characterization of peptide cross-β assemblies have enabled the design and construction of robust asymmetric bilayer peptide membranes. Two peptides differing only in their N-terminal residue, phosphotyrosine vs lysine, coassemble as stacks of antiparallel β-sheets with precisely patterned charged lattices stabilizing the bilayer leaflet interface. Either homogeneous or mixed leaflet composition is possible, and both create nanotubes with dense negative external and positive internal solvent exposed surfaces. Cross-seeding peptide solutions with a preassembled peptide nanotube seed leads to domains of different leaflet architecture within single nanotubes. Architectural control over these cross-β assemblies, both across the bilayer membrane and along the nanotube length, provides access to highly ordered asymmetric membranes for the further construction of functional mesoscale assemblies.
authors.affiliation: Departments of Biology and Chemistry, ‡Emory NMR Center, ⊥Emory Integrated Cellular Imaging Core, Emory University , Atlanta, Georgia 30322, United States.|School of Chemistry and Biochemistry and ∥School of Physics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.affiliation: Departments of Biology and Chemistry, ‡Emory NMR Center, ⊥Emory Integrated Cellular Imaging Core, Emory University , Atlanta, Georgia 30322, United States.|School of Chemistry and Biochemistry and ∥School of Physics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: Anil K
authors.initials: AK
authors.lastname: Mehta
authors.affiliation: Departments of Biology and Chemistry, ‡Emory NMR Center, ⊥Emory Integrated Cellular Imaging Core, Emory University , Atlanta, Georgia 30322, United States.|School of Chemistry and Biochemistry and ∥School of Physics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: Anton N
authors.initials: AN
authors.lastname: Sidorov
authors.affiliation: Departments of Biology and Chemistry, ‡Emory NMR Center, ⊥Emory Integrated Cellular Imaging Core, Emory University , Atlanta, Georgia 30322, United States.|School of Chemistry and Biochemistry and ∥School of Physics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Orlando
authors.affiliation: Departments of Biology and Chemistry, ‡Emory NMR Center, ⊥Emory Integrated Cellular Imaging Core, Emory University , Atlanta, Georgia 30322, United States.|School of Chemistry and Biochemistry and ∥School of Physics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Departments of Biology and Chemistry, ‡Emory NMR Center, ⊥Emory Integrated Cellular Imaging Core, Emory University , Atlanta, Georgia 30322, United States.|School of Chemistry and Biochemistry and ∥School of Physics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: Neil R
authors.initials: NR
authors.lastname: Anthony
authors.affiliation: Departments of Biology and Chemistry, ‡Emory NMR Center, ⊥Emory Integrated Cellular Imaging Core, Emory University , Atlanta, Georgia 30322, United States.|School of Chemistry and Biochemistry and ∥School of Physics, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
authors.forename: David G
authors.initials: DG
authors.lastname: Lynn
mesh_terms: D008565:Membrane Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D045333:Nanotubes, Peptide / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D000072417:Protein Domains
chemical_list: D008565:Membrane Proteins; D045333:Nanotubes, Peptide; D010455:Peptides