title: Simultaneous expression and transportation of insulin by supramolecular polysaccharide nanocluster.
abstract: Drug/gene transportation systems with stimuli-responsive release behaviors are becoming research hotspots in biochemical and biomedical fields. In this work, a glucose-responsive supramolecular nanocluster was successfully constructed by the intermolecular complexation of phenylboronic acid modified β-cyclodextrin with adamantane modified polyethylenimine, which could be used as a biocompatible carrier for insulin and pCMV3-C-GFPSpark-Ins DNA which could express insulin co-delivery. Benefiting from the response capability of phenylboronic acid moiety toward glucose, the encapsulated insulin could be specifically released and the corresponding targeted DNA could efficiently express insulin in HepG2 cell, accompanied by the high-level insulin release in vitro. Our results demonstrate that the simultaneous insulin drug delivery and insulin gene transfection in a controlled mode may have great potential in the clinical diabetes treatments.
authors.affiliation: Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071 P. R. China.
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071 P. R. China.
authors.forename: Ying-Ming
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071 P. R. China.
authors.forename: Qi-Hui
authors.initials: QH
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071 P. R. China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071 P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001692:Biological Transport; D004337:Drug Carriers; D015870:Gene Expression / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents; D007328:Insulin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D000078790:Insulin Secretion; D053758:Nanoparticles / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D007004:Hypoglycemic Agents; D007328:Insulin; D011134:Polysaccharides; D005947:Glucose