title: A T7exonuclease-assisted target recycling amplification with graphene oxide acting as the signal amplifier for fluorescence polarization detection of human immunodeficiency virus (HIV) DNA.
abstract: We report a fluorescence polarization (FP) platform for human immunodeficiency virus (HIV) DNA detection based on T7exonuclease-assisted target recycling amplification with graphene oxide (GO) acting as a FP signal amplifier. In the sensing method, the presence of the target DNA leads to target recycling with the assistance of T7exonuclease, furthermore, the amplification products are absorbed onto the surface of GO, so the all FP values are enhanced by GO. More importantly, this FP sensor exhibits high detection sensitivity; under optimal conditions, the change in FP is linear with the concentration of the target DNA within a concentration range of 50-2000 pmol/L, and the detection limit of this method is as low as 38.6 pmol/L. This FP sensor also exhibits high selectivity, even single-base mismatched DNA can be effectively discriminated from complementary target DNA. Above all, the proposed FP sensor may serve as a general platform for the sensitive assay of disease-related genes.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Education Ministry, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Education Ministry, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Jianniao
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Education Ministry, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Education Ministry, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources of Education Ministry, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D004279:DNA, Viral / Q000032:analysis*; D005090:Exodeoxyribonucleases / Q000737:chemistry / Q000378:metabolism*; D005454:Fluorescence Polarization; D006108:Graphite / Q000737:chemistry*; D006678:HIV / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*
chemical_list: D004279:DNA, Viral; D010087:Oxides; D006108:Graphite; D005090:Exodeoxyribonucleases; C046264:gene b exonuclease
keywords: HIV DNA; T7exonuclease; fluorescence polarization; graphene oxide; target recycling