title: Comparative proteomic analysis of the saliva of the Rhodnius prolixus, Triatoma lecticularia and Panstrongylus herreri triatomines reveals a high interespecific functional biodiversity.
abstract: Triatomines are hematophagous arthropods that transmit Trypanosoma cruzi and Trypanosoma rangeli. Feeding behavior and pathogen transmission is known to vary between the different species, and this characteristic is directly or indirectly dependent on the bioactive molecules of the saliva that facilitate the vector-host-parasite interaction. Here, we identify, characterize and compare the sialoproteomic (from the Greek sialo: saliva) repertoire of important species of the main triatomine genera in the Americas (Rhodnius prolixus, Triatoma lecticularia and Panstrongylus herreri) to better explain this interaction through two-dimensional electrophoresis and mass spectrometry. We identified 221 proteins, 69 from R. prolixus, 100 from T. lecticularia and 52 from P. herreri. We identified high abundance molecules with a great potential to modulate host defenses and homeostasis, highlighting Nitrophorin-4 (28.7%), Salivary lipocalin-5 (65.2%) and Putative triabin (20.5%) in R. prolixus, T. lecticularia and P. herreri, respectively. We also observed that only a single hypothetical protein is shared among three species, which was not functionally categorized. This study corroborates previous findings with R. prolixus, increasing the knowledge about this species with relevant proteomic information and comparisons with the other two targets of the study, T. lecticularia and P. herreri, for which no studies are available from a proteomics perspective.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: em.carlos1984@gmail.com.
authors.forename: Carlos Emmanuel
authors.initials: CE
authors.lastname: Montandon
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil; Biomolecules Analysis Center, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: edvaldo.barros@ufv.br.
authors.forename: Edvaldo
authors.initials: E
authors.lastname: Barros
authors.affiliation: Biomolecules Analysis Center, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: pedro.vidigal@ufv.br.
authors.forename: Pedro Marcus
authors.initials: PM
authors.lastname: Vidigal
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, Brazil. Electronic address: mariatays88@yahoo.com.br.
authors.forename: Maria Tays
authors.initials: MT
authors.lastname: Mendes
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, Brazil. Electronic address: anacbanhe@yahoo.com.br.
authors.forename: Ana Carolina Borella Marfil
authors.initials: AC
authors.lastname: Anhê
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: humramos@ufv.br.
authors.forename: Humberto Josué
authors.initials: HJ
authors.lastname: de Oliveira Ramos
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Federal University of Triangulo Mineiro, Uberaba, Minas Gerais, Brazil. Electronic address: carlo@icbn.uftm.edu.br.
authors.forename: Carlo José Freire
authors.initials: CJ
authors.lastname: de Oliveira
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: mafra@ufv.br.
authors.forename: Cláudio
authors.initials: C
authors.lastname: Mafra
mesh_terms: D000818:Animals; D044822:Biodiversity; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007303:Insect Vectors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D010203:Panstrongylus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012239:Rhodnius / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012463:Saliva / Q000737:chemistry / Q000378:metabolism; D014224:Triatoma / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D019476:Insect Proteins
keywords: Panstrongylus herreri; Rhodnius prolixus; Sialoproteomics; Triatoma lecticularia; Triatomines