title: Structure and function of the AAA+ ATPase p97/Cdc48p.
abstract: p97 (also known as valosin-containing protein (VCP) in mammals or Cdc48p in Saccharomyces cerevisiae) is an evolutionarily conserved ATPase present in all eukaryotes and archaebacteria. In conjunction with a collection of cofactors and adaptors, p97/Cdc48p performs an array of biological functions mostly through modulating the stability of 'client' proteins. Using energy from ATP hydrolysis, p97/Cdc48p segregates these molecules from immobile cellular structures such as protein assemblies, membrane organelles, and chromatin. Consequently, the released polypeptides can be efficiently degraded by the ubiquitin proteasome system or recycled. This review summarizes our current understanding of the structure and function of this essential cellular chaperoning system.
authors.affiliation: Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: xiad@mail.nih.gov.
authors.forename: Di
authors.initials: D
authors.lastname: Xia
authors.affiliation: Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Wai Kwan
authors.initials: WK
authors.lastname: Tang
authors.affiliation: Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: yihongy@mail.nih.gov.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Ye
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D006706:Homeostasis; D006801:Humans; D006868:Hydrolysis; D008561:Membrane Fusion; D051379:Mice; D058990:Molecular Targeted Therapy; D009154:Mutation; D009369:Neoplasms / Q000188:drug therapy; D011487:Protein Conformation; D029701:Saccharomyces cerevisiae Proteins; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C000614177:CDC48 protein, S cerevisiae; C000614178:VCP protein, human; D000074405:Valosin Containing Protein; C000614179:Vcp protein, mouse
keywords: AAA+ ATPase; Chromatin-associated degradation; ER-associated degradation; Membrane fusion; Mitochondria-associated degradation; Proteasome; Protein quality control; Ribosome-associated degradation; Segregase; Ubiquitin; Unfoldase; p97/CDC48