title: EFFECTS CRYOPRESERVATION OF IONOTROPIC GLUTAMATERGIC RECEPTOR MECHANISMS IN VITRO.
abstract: BACKGROUND
  We assayed hypothesis of cryopreservation of small volumes of nervous tissue (olfactory cortex slices).
  
  
  OBJECTIVE
  Effect of freezing (-10(0)C) and rewarming to +37(0)C studied on glutamatergic mechanisms.
  
  
  METHODS
  Viability slices determined from the change of the amplitudes of glutamatergic synaptic transmission: action potential of lateral olfactory tract (AP LOT), α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPAR) and N-methyl-D-aspartate (NMDAR) receptor-dependent mechanisms. Effects of two rates freezing/rewarming: slow (0.1(0)C/min) and rapid (9.0(0)C/min) were studied.
  
  
  RESULTS
  Activity AP LOT and AMPARs restored at slow rate freezing/rewarming, and at rapid rate, these indicators were increased. Activity NMDARs decreased at slow rate both at slow and at rapid rates after freezing/rewarming were blocked. At rewarming water swelling content ofin slices was maximal at rapid rate of freezing/rewarming.
  
  
  CONCLUSIONS
  The protocol of cryopreservation with slow rate (0.1(0)C/min) freezing/rewarming and a depth of freezing -10(0)C is optimal for the cryopreservation of small volumes of nervous tissue.
authors.affiliation: I. P. Pavlov Institute of Physiology, Russian Academy of Science, Saint-Petersburg, Russia. mok@inbox.ru.
authors.forename: A A
authors.initials: AA
authors.lastname: Mokrushin
mesh_terms: D000200:Action Potentials; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D007700:Kinetics; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009830:Olfactory Bulb / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000378:metabolism*; D017694:Rewarming
chemical_list: C096392:Grin3a protein, rat; D008562:Membrane Glycoproteins; D018091:Receptors, AMPA