title: Faecal chitinase 3-like 1 is a reliable marker as accurate as faecal calprotectin in detecting endoscopic activity in adult patients with inflammatory bowel diseases.
abstract: BACKGROUND
  Faecal biomarkers are emerging tools in the assessment of mucosal healing in inflammatory bowel diseases (IBDs).
  
  
  OBJECTIVE
  To evaluate the accuracy of faecal chitinase 3-like 1(CHI3L1) compared to calprotectin in detecting endoscopic activity in IBD.
  
  
  METHODS
  Overall, 86 IBD adults underwent colonoscopy consecutively and prospectively, with Crohn's disease Endoscopic Index of Severity (CDEIS) or Mayo endoscopic subscore calculation for ulcerative colitis, and stool collection. Faecal calprotectin was measured using quantitative immunochromatographic testing. Faecal CHI3L1 was quantified by ELISA. CHI3L1 cut-off value was determined using a receiver-operating curve.
  
  
  RESULTS
  In 54 Crohn's disease patients, faecal CHI3L1 (ρ = 0.70, P < 0.001) and calprotectin (ρ = 0.74, P < 0.001) levels correlated with CDEIS and were significantly increased in patients with endoscopic ulceration. In patients with ileal Crohn's disease, faecal CHI3L1 seemed to be better correlated with CDEIS than faecal calprotectin (ρ = 0.78 vs. ρ = 0.62, P < 0.001 for both). CHI3L1 > 15 ng/g detected endoscopic ulceration in Crohn's disease with a sensitivity of 100% and a specificity of 63.6%, compared to faecal calprotectin > 250 μg/g showing a sensitivity of 90.5% and a specificity of 59.1%. In 32 ulcerative colitis patients, faecal CHI3L1 and calprotectin levels correlated with Mayo endoscopic subscore (ρ = 0.44 and 0.61, respectively, P < 0.001 for both) and were significantly increased in ulcerative colitis patients with endoscopic activity. In ulcerative colitis patients, faecal CHI3L1 > 15 ng/g predicted endoscopic activity with a sensitivity of 81.8% and a specificity of 80.0%, compared to faecal calprotectin>250 μg/g showing a sensitivity of 86.4% and a specificity of 80.0%.
  
  
  CONCLUSIONS
  Faecal CHI3L1 is a reliable biomarker in detecting endoscopic activity in IBD.
authors.affiliation: Gastroenterology Department, University Hospital Estaing, Clermont-Ferrand, France.|Microbes, Intestine, Inflammation and Susceptibility of the Host, UMR 1071 Inserm/Université d'Auvergne, USC-INRA 2018, Clermont-Ferrand, France.
authors.forename: A
authors.initials: A
authors.lastname: Buisson
authors.affiliation: Gastroenterology Department, University Hospital Estaing, Clermont-Ferrand, France.|Microbes, Intestine, Inflammation and Susceptibility of the Host, UMR 1071 Inserm/Université d'Auvergne, USC-INRA 2018, Clermont-Ferrand, France.
authors.forename: E
authors.initials: E
authors.lastname: Vazeille
authors.affiliation: Biochemistry Laboratory, University Hospital G. Montpied, Clermont-Ferrand, France.
authors.forename: R
authors.initials: R
authors.lastname: Minet-Quinard
authors.affiliation: Gastroenterology Department, University Hospital Estaing, Clermont-Ferrand, France.|Microbes, Intestine, Inflammation and Susceptibility of the Host, UMR 1071 Inserm/Université d'Auvergne, USC-INRA 2018, Clermont-Ferrand, France.
authors.forename: M
authors.initials: M
authors.lastname: Goutte
authors.affiliation: Biochemistry Laboratory, University Hospital G. Montpied, Clermont-Ferrand, France.
authors.forename: D
authors.initials: D
authors.lastname: Bouvier
authors.affiliation: Gastroenterology Department, University Hospital Estaing, Clermont-Ferrand, France.
authors.forename: F
authors.initials: F
authors.lastname: Goutorbe
authors.affiliation: Biostatistics Unit- DRCI, GM - Clermont-Ferrand University and Medical Center, Clermont-Ferrand, France.
authors.forename: B
authors.initials: B
authors.lastname: Pereira
authors.affiliation: Microbes, Intestine, Inflammation and Susceptibility of the Host, UMR 1071 Inserm/Université d'Auvergne, USC-INRA 2018, Clermont-Ferrand, France.
authors.forename: N
authors.initials: N
authors.lastname: Barnich
authors.affiliation: Gastroenterology Department, University Hospital Estaing, Clermont-Ferrand, France.|Microbes, Intestine, Inflammation and Susceptibility of the Host, UMR 1071 Inserm/Université d'Auvergne, USC-INRA 2018, Clermont-Ferrand, France.
authors.forename: G
authors.initials: G
authors.lastname: Bommelaer
mesh_terms: D054392:Adipokines / Q000032:analysis*; D000328:Adult; D015415:Biomarkers; D000071451:Chitinase-3-Like Protein 1; D003093:Colitis, Ulcerative / Q000503:physiopathology; D003113:Colonoscopy; D003424:Crohn Disease / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D007082:Ileum; D015212:Inflammatory Bowel Diseases / Q000503:physiopathology*; D007413:Intestinal Mucosa / Q000503:physiopathology; D037102:Lectins / Q000032:analysis*; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
chemical_list: D054392:Adipokines; D015415:Biomarkers; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; D037102:Lectins; D039841:Leukocyte L1 Antigen Complex