title: UHPLC/ESI-MS/MS Determination of 187 Pesticides in Wine.
abstract: This paper presents an ultra HPLC/electrospray ionization-tandem MS method to determine pesticides in wine. We adopted the quick, easy, cheap, effective, rugged, and safe (QuEChERs) method for extraction and used core-shell column to achieve ultra-HPLC to develop and validate a simple and fast method to analyze 187 pesticide residues in red and white wine samples. Pesticide residues were extracted from wine samples using QuEChERS. Ultra HPLC/electrospray ionization-tandem MS quantification was achieved using matrix-matched standard calibration curves with isotopically labeled standards or a chemical analogue as internal standards with an analytical range from 5.0 to 500.0 μg/L. The method performance characteristics that included overall recovery, intermediate precision, and measurement uncertainty were evaluated according to a nested experimental design. Generally, 98.4% (in red wine) and 96.8% (in white wine) of the pesticides had recoveries between 71 and 120%; 98.9% (in red wine) and 99.5% (in white wine) of the pesticides had the intermediate precision ≤20%; and 99.5% (in red wine) and 98.4% (in white wine) of the pesticides had measurement uncertainty ≤50%.
authors.affiliation: Canadian Food Inspection Agency, Calgary Laboratory, 3650-36th St N.W., Calgary, Alberta, Canada T2L 2L1.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Wendy
authors.initials: W
authors.lastname: Cheung
mesh_terms: D002851:Chromatography, High Pressure Liquid; D010575:Pesticides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014920:Wine / Q000032:analysis*
chemical_list: D010575:Pesticides