title: Sortilin is associated with the chlamydial inclusion and is modulated during infection.
abstract: Chlamydia species are obligate intracellular pathogens that have a major impact on human health. The pathogen replicates within an intracellular niche called an inclusion and is thought to rely heavily on host-derived proteins and lipids, including ceramide. Sortilin is a transmembrane receptor implicated in the trafficking of acid sphingomyelinase, which is responsible for catalysing the breakdown of sphingomyelin to ceramide. In this study, we examined the role of sortilin in Chlamydia trachomatis L2 development. Western immunoblotting and immunocytochemistry analysis revealed that endogenous sortilin is not only associated with the inclusion, but that protein levels increase in infected cells. RNAi-mediated depletion of sortilin, however, had no detectable impact on ceramide delivery to the inclusion or the production of infectious progeny. This study demonstrates that whilst Chlamydia redirects sortilin trafficking to the chlamydial inclusion, RNAi knockdown of sortilin expression is insufficient to determine if this pathway is requisite for the development of the pathogen.
authors.affiliation: Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4067, Australia.
authors.forename: Wei Xuan
authors.initials: WX
authors.lastname: Teo
authors.affiliation: Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4067, Australia.
authors.forename: Markus Charles
authors.initials: MC
authors.lastname: Kerr
authors.affiliation: School of Life Sciences, University of Technology Sydney, Broadway, New South Wales 2007, Australia.
authors.forename: Wilhelmina May
authors.initials: WM
authors.lastname: Huston
authors.affiliation: Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4067, Australia R.Teasdale@uq.edu.au.
authors.forename: Rohan David
authors.initials: RD
authors.lastname: Teasdale
keywords: Acid sphingomyelinase; Ceramide; Chlamydia trachomatis; Sortilin