title: Vectorization of Nucleic Acids for Therapeutic Approach: Tutorial Review.
abstract: Oligonucleotides present a high therapeutic potential for a wide variety of diseases. However, their clinical development is limited by their degradation by nucleases and their poor blood circulation time. Depending on the administration mode and the cellular target, these macromolecules will have to cross the vascular endothelium, to diffuse through the extracellular matrix, to be transported through the cell membrane, and finally to reach the cytoplasm. To overcome these physiological barriers, many strategies have been developed. Here, we review different methods of DNA vectorization, discuss limitations and advantages of the various vectors, and provide new perspectives for future development.
authors.affiliation: Laboratoire CSPBAT, CNRS UMR 7244, UFR SMBH, Université Paris 13 , Sorbonne Paris Cité, F-93017 Bobigny, France.
authors.forename: Frederic
authors.initials: F
authors.lastname: Geinguenaud
authors.affiliation: Inserm, U1148, Laboratory for Vascular Translational Science, UFR SMBH, Université Paris 13 , Sorbonne Paris Cité, F-93017 Bobigny, France.
authors.forename: Erwann
authors.initials: E
authors.lastname: Guenin
authors.affiliation: Inserm, U1148, Laboratory for Vascular Translational Science, UFR SMBH, Université Paris 13 , Sorbonne Paris Cité, F-93017 Bobigny, France.|Service de Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris 93009 Bobigny France.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Lalatonne
authors.affiliation: Inserm, U1148, Laboratory for Vascular Translational Science, UFR SMBH, Université Paris 13 , Sorbonne Paris Cité, F-93017 Bobigny, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Motte
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods; D018014:Gene Transfer Techniques / Q000295:instrumentation; D006801:Humans; D009696:Nucleic Acids / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D009841:Oligonucleotides / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use
chemical_list: D009696:Nucleic Acids; D009841:Oligonucleotides