title: μFE models can represent microdamaged regions of healthy and metastatically involved whole vertebrae identified through histology and contrast enhanced μCT imaging.
abstract: Micro-damage formation within the skeleton is an important stimulant for bone remodeling, however abnormal build-up of micro-damage can lead to skeletal fragility. In this study, µCT imaging based micro finite element (μFE) models were used to evaluate tissue level damage criteria in whole healthy and metastatically-involved vertebrae. T13-L2 spinal segments were excised from osteolytic (n=3) and healthy (n=3) female athymic rnu/rnu rats. Osteolytic metastasis was generated by intercardiac injection of HeLa cancer cells. Micro-mechanical axial loading was applied to the spinal motion segments under μCT imaging. Vertebral samples underwent BaSO4 staining and sequential calcein/fuchsin staining to identify load induced micro-damage. μCT imaging was used generate specimen specific μFE models of the healthy and osteolytic whole rat vertebrae. Model boundary conditions were generated through deformable image registration of loaded and unloaded scans. Elevated stresses and strains were detected in regions of micro-damage identified through histological and BaSO4 staining within healthy and osteolytic vertebral models, as compared to undamaged regions. Additionally, damaged regions of metastatic vertebrae experienced significantly higher local stresses and strains than those in the damaged regions of healthy specimens. Areas identified by BaSO4 staining, however, yielded lower levels of stress and strain in damaged and undamaged regions of healthy and metastatic vertebrae as compared to fuschin staining. The multimodal (experimental, image-based and computational) techniques used in this study demonstrated the ability of local stresses and strains computed through µFE analysis to identify trabecular micro-damage, that can be applied to biomechanical analyses of healthy and diseased whole bones.
authors.affiliation: Sunnybrook Research Institute, Toronto, ON, Canada; Institute for Biomaterials and Biomedical Engineering, Toronto, ON, Canada.
authors.forename: Chetan
authors.initials: C
authors.lastname: Choudhari
authors.affiliation: Sunnybrook Research Institute, Toronto, ON, Canada.
authors.forename: Katelyn
authors.initials: K
authors.lastname: Chan
authors.affiliation: TECHNA Institute, University Health Network, Toronto, ON, Canada; Department of Surgery, Toronto, ON, Canada.
authors.forename: Margarete K
authors.initials: MK
authors.lastname: Akens
authors.affiliation: Sunnybrook Research Institute, Toronto, ON, Canada; Department of Surgery, Toronto, ON, Canada; Institute for Biomaterials and Biomedical Engineering, Toronto, ON, Canada. Electronic address: cari.whyne@sunnybrook.ca.
authors.forename: Cari M
authors.initials: CM
authors.lastname: Whyne
mesh_terms: D000818:Animals; D003287:Contrast Media; D005260:Female; D020342:Finite Element Analysis; D006367:HeLa Cells; D006801:Humans; D009362:Neoplasm Metastasis; D010014:Osteolysis / Q000000981:diagnostic imaging / Q000473:pathology; D051381:Rats; D013131:Spine / Q000000981:diagnostic imaging* / Q000473:pathology*; D055114:X-Ray Microtomography
chemical_list: D003287:Contrast Media
keywords: Barium sulfate; Biomechanics; Finite element analysis; Micro computed tomography; Micro-damage; Sequential staining