title: Capsular Typing Method for Streptococcus agalactiae Using Whole-Genome Sequence Data.
abstract: Group B streptococcus (GBS) capsular serotypes are major determinants of virulence and affect potential vaccine coverage. Here we report a whole-genome-sequencing-based method for GBS serotype assignment. This method shows strong agreement (kappa of 0.92) with conventional methods and increased serotype assignment (100%) to all 10 capsular types.
authors.affiliation: Modernising Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom anna.sheppard@ndm.ox.ac.uk aseale@nhs.net.
authors.forename: Anna E
authors.initials: AE
authors.lastname: Sheppard
authors.affiliation: Modernising Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: Alison
authors.initials: A
authors.lastname: Vaughan
authors.affiliation: Modernising Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: Nicola
authors.initials: N
authors.lastname: Jones
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom Shoklo Malaria Research Unit, Mae Sot, Thailand.
authors.forename: Paul
authors.initials: P
authors.lastname: Turner
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom Shoklo Malaria Research Unit, Mae Sot, Thailand.
authors.forename: Claudia
authors.initials: C
authors.lastname: Turner
authors.affiliation: Microbiology Reference Division, Public Health England, London, United Kingdom Imperial College, London, United Kingdom.
authors.forename: Androulla
authors.initials: A
authors.lastname: Efstratiou
authors.affiliation: Microbiology Reference Division, Public Health England, London, United Kingdom.
authors.forename: Darshana
authors.initials: D
authors.lastname: Patel
authors.affiliation: Modernising Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: A Sarah
authors.initials: AS
authors.lastname: Walker
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.
authors.forename: James A
authors.initials: JA
authors.lastname: Berkley
authors.affiliation: Modernising Medical Microbiology Consortium, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom.
authors.forename: Derrick W
authors.initials: DW
authors.lastname: Crook
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya anna.sheppard@ndm.ox.ac.uk aseale@nhs.net.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Seale
mesh_terms: D016667:Bacterial Capsules / Q000235:genetics*; D016680:Genome, Bacterial; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D017422:Sequence Analysis, DNA; D012703:Serotyping / Q000379:methods; D013292:Streptococcus agalactiae / Q000145:classification* / Q000235:genetics*