title: Enhanced efficacy and anti-biofilm activity of novel nanoemulsions against skin burn wound multi-drug resistant MRSA infections.
abstract: Multi-drug resistant MRSA (methicillin-resistant Staphylococcus aureus) is a global problem for human health, especially skin burn wound patients. Therefore, we estimated the antibacterial and anti-biofilm activity of a chlorhexidine acetate nanoemulsion (CNE) by previously ourselves designed against skin burn wound MRSA infections. Compared with its water solution (CHX), CNE showed a better and faster action against MRSA both in vitro and in vivo. Importantly, CNE was more effective at inhibiting biofilm formation and clearing the biofilm. We also found that the cell walls and membranes of MRSA were severely disrupted after treatment with CNE. Moreover, the relative electrical conductivity and the leakage of alkaline phosphates, K(+), Mg(2+), DNA and protein obviously increased because the cell wall and membrane were damaged. These data show that novel CNE is a promising potential antimicrobial candidate, especially for skin burn wound MRSA infections.
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Song
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China. Electronic address: sunhongwu2001@163.com.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Sun
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Haiming
authors.initials: H
authors.lastname: Jing
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Liuyang
authors.initials: L
authors.lastname: Yang
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Tong
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wei
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Zelin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China.
authors.forename: Quanming
authors.initials: Q
authors.lastname: Zou
authors.affiliation: National Engineering Research Center of Immunological Products & Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA, Chongqing, PR China. Electronic address: zeng1109@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Zeng
mesh_terms: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000008:administration & dosage*; D018441:Biofilms; D002056:Burns / Q000188:drug therapy / Q000382:microbiology*; D004655:Emulsions; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus; D053758:Nanoparticles; D013203:Staphylococcal Infections
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D004655:Emulsions
keywords: Anti-biofilm activity; Burn wound skin infection mouse model; Chlorhexidine acetate; Methicillin-resistant Staphylococcus aureus; Nanoemulsion