title: Evaluation of the Cytotoxic Behavior of Fungal Extracellular Synthesized Ag Nanoparticles Using Confocal Laser Scanning Microscope.
abstract: Silver nanoparticles have been synthesized by subjecting a reaction medium to a Fusarium oxysporum biomass at 28 °C for 96 h. The biosynthesized Ag nanoparticles were characterized on the basis of their anticipated peak at 405 nm using UV-Vis-NIR spectroscopy. Structural confirmation was evident from the characteristic X-ray diffraction (XRD) pattern, high-resolution transmission electron Microscopy (HRTEM) and the particle size analyzer. The Ag nanoparticles were of dimension 40 ± 5 nm and spherical in shape. The study mainly focused on using the confocal laser scanning microscope (CLSM) to examine the cytotoxic activities of fungal synthesized Ag nanoparticles on a human breast carcinoma cell line MCF7 cell, which featured remarkable vacuolation, thus indicating a potent cytotoxic activity.
authors.affiliation: Nanotechnology and Advanced Materials Central Lab, Agriculture Research Center, PO Box 588 Orman, Giza 12619, Egypt. t1salah@hotmail.com.
authors.forename: Taher A
authors.initials: TA
authors.lastname: Salaheldin
authors.affiliation: Faculty of Women for Art, Science &amp; Education, Ain Shams University, PO Box 11757 Alkurba, Cairo 11341, Egypt. husseinymoussa@women.asu.edu.eg.
authors.forename: Sherif M
authors.initials: SM
authors.lastname: Husseiny
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. amenizi@ksu.edu.sa.
authors.forename: Abdullah M
authors.initials: AM
authors.lastname: Al-Enizi
authors.affiliation: Materials Science and Technology Program, College of Arts and Sciences, Qatar University, PO Box 2713, Doha, Qatar. aelzatahry@qu.edu.qa.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Elzatahry
authors.affiliation: Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA. acowley@cm.utexas.edu.
authors.forename: Alan H
authors.initials: AH
authors.lastname: Cowley
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001940:Breast / Q000187:drug effects / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005670:Fusarium / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D036103:Nanotechnology; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D012834:Silver
keywords: breast cancer; confocal laser scanning microcopy; cytotoxicity; silver nanoparticles