title: Engrailed-2 might play an anti-oncogenic role in clear-cell renal cell carcinoma.
abstract: Our preliminary study indicated that Engrailed-2 (EN2) is downregulated but also ectopically expressed in clear-cell renal cell carcinoma (CCRCC), and the absence of EN2 expression was associated with poor histological grade. However, the specific roles of EN2 in CCRCC have yet to be elucidated. In the present study, we examined the effects of inhibiting EN2 expression by human renal tubular epithelial cells (HK-2) and overexpressing EN2 by human clear-cell renal cells (786-O). Results showed that EN2 inhibition accelerated HK-2 cell proliferation, shortened the cell cycle, reduced apoptosis, and acted more invasively. By contrast, EN2 overexpression in 786-O cells decelerated the proliferative ability of 786-O, increased the percentage of cell apoptosis, and weakened the invasive ability. Overall, the results demonstrated that EN2 might play an anti-oncogenic role in oncogenesis and development of CCRCC, thereby maintaining the normal growth of human renal tubular epithelial cells.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.|Department of Urology, The First Affiliated Hospital of Gannan Medical College, Ganzhou, 341000, China.
authors.forename: Cai-Yong
authors.initials: CY
authors.lastname: Lai
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.
authors.forename: Gan-Shen
authors.initials: GS
authors.lastname: Yu
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Li
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China.
authors.forename: Bao-Li
authors.initials: BL
authors.lastname: Heng
authors.affiliation: Department of Urology, The First Affiliated Hospital of Gannan Medical College, Ganzhou, 341000, China.
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Xue
authors.affiliation: Department of Urology, The First Affiliated Hospital of Jinan University, No. 613 Huang Pu Rd, Guangzhou, 510630, China. 541162750@qq.com.
authors.forename: Ze-Xuan
authors.initials: ZX
authors.lastname: Su
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000099266:Engrailed 2 Protein
chemical_list: D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000099266:Engrailed 2 Protein
keywords: Anti-oncogene; Clear-cell renal cell carcinoma; Engrailed-2