title: Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization.
abstract: Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
authors.affiliation: UC Davis, School of Veterinary Medicine, Department of Pathology, Microbiology, and Immunology, USA.
authors.forename: Molly E
authors.initials: ME
authors.lastname: Church
authors.affiliation: UC Davis, School of Veterinary Medicine, Department of Pathology, Microbiology, and Immunology, USA.
authors.forename: Florante N
authors.initials: FN
authors.lastname: Dela Cruz
authors.affiliation: UC Davis, School of Medicine, Department of Dermatology, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Kim
authors.affiliation: UC Davis, School of Veterinary Medicine, California Animal Health and Food Safety Laboratory System, USA.
authors.forename: Michele
authors.initials: M
authors.lastname: Persiani
authors.affiliation: UC Davis, School of Veterinary Medicine, California Animal Health and Food Safety Laboratory System, USA.
authors.forename: Leslie W
authors.initials: LW
authors.lastname: Woods
authors.affiliation: UC Davis, School of Veterinary Medicine, Department of Pathology, Microbiology, and Immunology, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Pesavento
authors.affiliation: UC Davis, School of Veterinary Medicine, Department of Pathology, Microbiology, and Immunology, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Woolard