title: Identification of meat products by shotgun spectral matching.
abstract: A new method, based on shotgun spectral matching of peptide tandem mass spectra, was successfully applied to the identification of different food species. The method was demonstrated to work on raw as well as processed samples from 16 mammalian and 10 bird species by counting spectral matches to spectral libraries in a reference database with one spectral library per species. A phylogenetic tree could also be constructed directly from the spectra. Nearly all samples could be correctly identified at the species level, and 100% at the genus level. The method does not use any genomic information and unlike targeted methods, no prior knowledge of genetic variation within a genus or species is necessary.
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2300 ZC Leiden, The Netherlands. Electronic address: d.ohana@lumc.nl.
authors.forename: D
authors.initials: D
authors.lastname: Ohana
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2300 ZC Leiden, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Dalebout
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2300 ZC Leiden, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Marissen
authors.affiliation: The Novo Nordisk Foundation Center for Biosustainability (DTU Biosustain), Kogle Alle 6, DK-2970 Hørsholm, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Wulff
authors.affiliation: Analytical Chemistry, Department of Chemistry - Biomedical Centre and SciLifeLab, Uppsala University, Uppsala, Husargatan 3, 751 24 Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Bergquist
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2300 ZC Leiden, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Deelder
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2300 ZC Leiden, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Palmblad
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005504:Food Analysis / Q000379:methods*; D005515:Food Labeling; D008461:Meat Products / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D010802:Phylogeny; D011200:Poultry; D040901:Proteomics / Q000379:methods*; D012418:Ruminants; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D010455:Peptides
keywords: Food products; Identification; Quantitation; Shotgun proteomics; Spectral libraries