title: Drug functionalized microbial polysaccharide based nanofibers as transdermal substitute.
abstract: In order to promote the natural healing process, drug-functionalized nanofibrous transdermal substitute was fabricated using gellan as chief polymer and polyvinyl alcohol (PVA) as supporting polymer via electrospinning technique. These fabricated nanofibers physiochemically mimic the extracellular matrix (ECM) which supports the cell growth. For neo-tissue regeneration in a sterilized environment, amoxicillin (Amx) was entrapped within these nanofibers. Entrapment of Amx in the nanofibers was confirmed by FESEM, FTIR, XRD and TG analysis. In vitro cell culture studies revealed that the fabricated non-cytotoxic nanofibers promoted enhance cell adherence and proliferation of human keratinocytes. A preliminary in vivo study performed on rat model for full thickness skin excision wound demonstrated the prompt re-epithelialization in early phase and quicker collagen deposition in later phases of wound healing in case of Amx-functionalized gellan/PVA nanofibers. Data collectively confirmed the potential usage of gellan based electrospun nanofibers as transdermal substitute for faster skin restoration.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Vashisth
authors.affiliation: Sapience Bio-Analytical Research Lab Bhopal, Madhya Pradesh, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: Sapience Bio-Analytical Research Lab Bhopal, Madhya Pradesh, India.
authors.forename: Hemant
authors.initials: H
authors.lastname: Nagar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India; Sapience Bio-Analytical Research Lab Bhopal, Madhya Pradesh, India.
authors.forename: Navdeep
authors.initials: N
authors.lastname: Raghuwanshi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
authors.forename: Shruti
authors.initials: S
authors.lastname: Sharan
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
authors.forename: Kumar
authors.initials: K
authors.lastname: Nikhil
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
authors.forename: Parul A
authors.initials: PA
authors.lastname: Pruthi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
authors.forename: Rajesh P
authors.initials: RP
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.
authors.forename: Partha
authors.initials: P
authors.lastname: Roy
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India. Electronic address: vikasfbs@iitr.ernet.in.
authors.forename: Vikas
authors.initials: V
authors.lastname: Pruthi
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D003094:Collagen; D006801:Humans; D057139:Nanofibers; D011142:Polyvinyl Alcohol; D051381:Rats; D012038:Regeneration; D012879:Skin Physiological Phenomena; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D011142:Polyvinyl Alcohol; D003094:Collagen
keywords: Electrospinning; Gellan; Nanofibers; Transdermal substitute; Wound healing