title: Protective effects of sevoflurane in hepatic ischemia-reperfusion injury.
abstract: The endothelial glycocalyx plays a critical role in the regulation of vascular structure and functions. Previous studies have demonstrated that sevoflurane, a volatile anesthetic, can preserve the endothelial glycocalyx in heart tissues against ischemia-reperfusion injury. However, little is known about the effects of sevoflurane pretreatment on the vascular structure and functions of liver tissues following ischemia-reperfusion injury. To this end, female Sprague-Dawley rats (n = 28) were anesthetized either with ketamine (80-120 mg/kg, i.p.) or with one minimum alveolar concentration (MAC) sevoflurane (2% v/v). Following in vivo hepatic ischemia procedure, the liver was isolated and reperfusion was produced. During the period of reperfusion, liver reperfusion samples were collected, and the concentrations of heparan sulfate and syndecan-1 (Syn-1), and the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzymes, were measured. The morphology of hepatocytes and endothelial glycocalyx were then assessed by using the light and electron microscopies, respectively. Ischemia-reperfusion increased the release of HS and Syn-1, and elevated the levels of ALT and AST in a time-dependent manner. However, sevoflurane pretreatment reduced the release of HS and Syn-1and attenuated the levels of ALT and AST, in a time-dependent manner, as compared with ketamine pretreatment. Furthermore, sevoflurane pretreatment decreased the shedding of endothelial glycocalyx and hepatocytes necrosis. Sevoflurane pretreatment preserved the endothelial glycocalyx in the liver tissue against ischemia-reperfusion injury. The effect appears to help protect hepatocytes against ischemia-reperfusion-induced necrosis.
authors.affiliation: Department of Anesthesiology, The First Hospital of Jilin University, Changchun, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The First Hospital of Jilin University, Changchun, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Yuan
authors.affiliation: Department of Pediatric Respiration, The First Hospital of Jilin University, Changchun, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University.
authors.forename: Guo-Yue
authors.initials: GY
authors.lastname: Lv
authors.affiliation: Department of Anesthesiology, The First Hospital of Jilin University, Changchun, China lhuaqi@hotmail.com.
authors.forename: Huan-Qiu
authors.initials: HQ
authors.lastname: Liu
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D019276:Glycocalyx / Q000378:metabolism; D008099:Liver / Q000187:drug effects; D008107:Liver Diseases / Q000188:drug therapy*; D008738:Methyl Ethers / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D020011:Protective Agents; D000077149:Sevoflurane; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: endothelial glycocalyx; heparan sulfate; hepatic ischemia-reperfusion; sevoflurane; syndecan-1