title: Dynamical Expression of MicroRNA-127-3p in Proliferating and Differentiating C2C12 Cells.
abstract: MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their individual roles are still not fully understood. In the present study, we investigated a muscle highly-expressed miRNA, miR-127-3p, in C2C12 myoblasts and tissues of goats with different muscle phenotypes (Boer vs Wushan black goats). Our results demonstrated that i) miR-127-3p was extensively expressed in tissues of goats; ii) miR-127-3p was higher expressed in muscle, spleen, heart, and skin in the muscular goats (Boer goats) than the control (Wushan black goats). Then we further characterized the dynamical expression of miR-127-3p,MyoD,MyoG,Myf5,Mef2c, andMyosinin the proliferating and differentiating C2C12 myoblasts at day of 0, 1, 3, 5, and 7 in culture mediums. Especially, we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells. Our findings suggest that miR-127-3p probably plays roles in the proliferation and differentiation of myoblasts, which further underlies regulation of muscle phenotype in goats.
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China.|Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Gaofu
authors.initials: G
authors.lastname: Wang
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Liangjia
authors.initials: L
authors.lastname: Liu
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.|College of Animal Science and Technology, Southwest University, Chongqing 400715, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
authors.forename: Youji
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China.
authors.forename: Hangxing
authors.initials: H
authors.lastname: Ren
keywords: C2C12; Differentiation; Goats; MiR-127-3p; Proliferation