title: The labeling of stem cells by superparamagnetic iron oxide nanoparticles modified with PEG/PVP or PEG/PEI.
abstract: Poly(ethylene glycol) (PEG) and poly(vinyl pyrrolidone) (PVP) co-modified superparamagnetic iron oxide nanoparticles (SPIONs) (PEG/PVP-SPIONs), and PEG and poly(ethylene imine) (PEI) co-modified SPIONs (PEG/PEI-SPIONs) synthesized by thermal decomposition have been used as magnetic resonance imaging (MRI) contrast agents to label adipose-derived stem cells (ADSCs). Efficient cell labeling was achieved after incubation with PEG/PVP-SPIONs and PEG/PEI-SPIONs for 12h, and the MRI of labeled cells was evaluated. The cell viability tests showed the low cytotoxicity of PEG/PVP-SPIONs and PEG/PEI-SPIONs. The cellular iron content incubated with PEG/PVP-SPIONs at a concentration of 25 μg/ml was 6.96 pg/cell, the cellular iron contents incubated with PEG/PEI-SPIONs at concentrations of 12 and 25 μg/ml were 20.16, 35.4 pg/cell, respectively. The SPIONs were located predominantly in the intracellular vesicles. The cellular iron oxide uptake was significantly high after incubation with PEG/PEI-SPIONs as compared with the commercial iron oxide agents (Feridex, Feridex@PLL, Resovist and Resovist@PLL) reported. This work demonstrates that PEG/PEI-SPIONs are the competent agents for the labeling of ADSCs.
authors.affiliation: State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, China; School of Materials Science and Engineering, Guilin University of Technology, Guilin 541004, Guangxi, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Imaging, Nan Sha Center Hospital, Guangzhou First Municipal People's Hospital, Guangzhou Medical University, Guangzhou 511457, Guangdong, China; Department of Radiology, Huizhou Municipal Central Hospital, Huizhou 516001, Guangdong, China.
authors.forename: Weiqiong
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, China; School of Materials Science and Engineering, Guilin University of Technology, Guilin 541004, Guangxi, China. Electronic address: baolinzhang@ymail.com.
authors.forename: Baolin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Imaging, Nan Sha Center Hospital, Guangzhou First Municipal People's Hospital, Guangzhou Medical University, Guangzhou 511457, Guangdong, China. Electronic address: xieqi8@yeah.net.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000818:Animals; D007097:Imines / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry*; D011205:Povidone / Q000031:analogs & derivatives* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D007097:Imines; D058185:Magnetite Nanoparticles; D011095:Polyethylenes; C505405:poly(ethylene imine); C077842:polyvinylpolypyrrolidone; D011092:Polyethylene Glycols; D011205:Povidone; C000595209:polyethylene glycol 1000
keywords: Cell labeling; MRI; PEI; SD-rat adipose derived stem cells (ADSCs); Superparamagnetic iron oxide nanoparticles (SPIONs)