title: EFFECT OF SEMEN EXTENDER ON PROTEIN CONCENTRATION IN EACH FRACTION OF CRYOPRESERVED HUMAN SEMEN.
abstract: BACKGROUND
  Semen extender is liquid diluents which are added to semen to preserve its fertilizing ability. The addition of extender to semen protects the sperm against possible damage by toxic seminal plasma, as well as providing nutrients.
  
  
  OBJECTIVE
  The hypotheses of the study are to evaluate, if there is any significant change in the protein concentration in each fractions of human semen before and after freezing with prepared semen extender. Then we are going to correlate the semen parameters with thee fresh semen total protein concentration.
  
  
  RESULTS
  After preserving with the extender, all the semen samples show statistically significant (p < 0.001) in case of protein concentration.
  
  
  CONCLUSIONS
  There is a significant different (p < 0.001) in protein concentration in various fractions before and after freezing. Also the prepared semen extender can be used in Assisted Reproductive centres (ART).
authors.affiliation: Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Vickram
authors.affiliation: Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.
authors.forename: Rao
authors.initials: R
authors.lastname: Kamini
authors.affiliation: Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.
authors.forename: M
authors.initials: M
authors.lastname: Ramesh Pathy
authors.affiliation: Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.
authors.forename: Thomas
authors.initials: T
authors.lastname: Chanchal
authors.affiliation: Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.
authors.forename: R
authors.initials: R
authors.lastname: Parameswari
authors.affiliation: Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India. tbsridharan@vit.ac.in.
authors.forename: T B
authors.initials: TB
authors.lastname: Sridharan
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011506:Proteins / Q000378:metabolism*; D012661:Semen / Q000737:chemistry; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*
chemical_list: D003451:Cryoprotective Agents; D011506:Proteins