title: LPS-induced neonatal stress in mice affects the response profile to an inflammatory stimulus in an age and sex-dependent manner.
abstract: The aim of this study is to evaluate the response to an inflammatory stimulus in mice exposed to LPS-induced neonatal stress at different ages and sexes. Balb/c mice were submitted to intraperitoneal injections on postnatal days 3 and 10 with lipopolysaccharide (nLPS) or saline solution (nSal). At 21 or 60 days, either saline solution was injected or an inflammatory stimulus was induced by the injection of 1% carrageenan. Inflammatory cytokines, reactive oxygen species, and neutrophil extracellular traps (NETs) production were measured in peritoneal fluid. LPS-induced neonatal stress can reduce inflammatory cytokines in males and females. An increase in NETs production was observed when 60 day nLPS animals were compared to 21 day mice in both sexes. The ROS production was not affected by neonatal stress. The results shown here indicate that LPS-induced neonatal stress can alter cytokine production in response to inflammatory stimuli at different ages, in a sex-dependent effect. © 2016 Wiley Periodicals, Inc. Dev Psychobiol 58: 600-613, 2016.
authors.affiliation: Centro Infant, Institute of Biomedical Research (IPB) Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690, 2° andar, Rio Grande do Sul, Porto Alegre, CEP 90610-000, Brazil.|Cellular and Molecular Biology Graduate Program, Faculty of Biosciences, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Barth
authors.affiliation: Centro Infant, Institute of Biomedical Research (IPB) Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690, 2° andar, Rio Grande do Sul, Porto Alegre, CEP 90610-000, Brazil.|Laboratory of Research in Cellular Biophysics and Inflammation, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Luft
authors.affiliation: Centro Infant, Institute of Biomedical Research (IPB) Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690, 2° andar, Rio Grande do Sul, Porto Alegre, CEP 90610-000, Brazil.|Cellular and Molecular Biology Graduate Program, Faculty of Biosciences, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: Giselle A
authors.initials: GA
authors.lastname: Funchal
authors.affiliation: Cellular and Molecular Biology Graduate Program, Faculty of Biosciences, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.|Laboratory of Research in Cellular Biophysics and Inflammation, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: Jarbas R
authors.initials: JR
authors.lastname: de Oliveira
authors.affiliation: Centro Infant, Institute of Biomedical Research (IPB) Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690, 2° andar, Rio Grande do Sul, Porto Alegre, CEP 90610-000, Brazil.
authors.forename: Bárbara N
authors.initials: BN
authors.lastname: Porto
authors.affiliation: Centro Infant, Institute of Biomedical Research (IPB) Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga, 6690, 2° andar, Rio Grande do Sul, Porto Alegre, CEP 90610-000, Brazil. mdonadio@pucrs.br.|Cellular and Molecular Biology Graduate Program, Faculty of Biosciences, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil. mdonadio@pucrs.br.|Laboratory of Research in Cellular Biophysics and Inflammation, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil. mdonadio@pucrs.br.
authors.forename: Márcio V F
authors.initials: MV
authors.lastname: Donadio
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001202:Ascitic Fluid / Q000378:metabolism*; D002351:Carrageenan / Q000008:administration & dosage; D016207:Cytokines / Q000378:metabolism*; D065206:Extracellular Traps / Q000378:metabolism*; D005260:Female; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012737:Sex Factors; D013312:Stress, Physiological / Q000276:immunology*; D048069:Tumor Necrosis Factors / Q000378:metabolism
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D048069:Tumor Necrosis Factors; D016753:Interleukin-10; D002351:Carrageenan
keywords: LPS; NETs; carrageenan; inflammation; neonatal stress