title: Preparation of biocompatible molecularly imprinted shell on superparamagnetic iron oxide nanoparticles for selective depletion of bovine hemoglobin in biological sample.
abstract: Bovine hemoglobin (BHb), as one of the high-abundance proteins, could seriously mask and hamper the analysis of low-abundance proteins in serum. To selectively deplete BHb, we design a simple and effective strategy for preparation of biocompatible molecularly imprinted shell on superparamagnetic iron oxide nanoparticles through surface imprinting technique combined with template immobilization strategy. Firstly, template proteins are immobilized on the directly aldehyde-functionalized magnetic nanoparticles through imine bonds. Then, with gelatin as functional monomer, a polymeric network molded around the immobilized template proteins is obtained. Finally, the specific cavities for BHb are fabricated after removing the template proteins. The effects of imprinting conditions were investigated and the optimal imprinting conditions are found to be 40mg of BHb, 150mg of gelatin, and 8h of polymerization time. The resultant materials exhibit good dispersion, high crystallinity, and satisfactory superparamagnetic property with a high saturation magnetization (33.43emug(-1)). The adsorption experiments show that the imprinted nanomaterials have high adsorption capacity of 93.1mgg(-1), fast equilibrium time of 35min, and satisfactory selectivity for target protein. Meanwhile, the obtained polymers could be used without obvious deterioration after six adsorption-desorption cycles. In addition, the resultant polymers are successfully applied in the selective isolation BHb from bovine blood sample, which could provide an alternative solution for the preparatory work of proteomics.
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: ruixiagao@mail.xjtu.edu.cn.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Gao
authors.affiliation: Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Dechun
authors.initials: D
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Multiphase Flow in Power Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Bianbian
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: guozj@mail.xjtu.edu.cn.
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D001769:Blood; D002417:Cattle; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D010316:Particle Size; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D006454:Hemoglobins; D058185:Magnetite Nanoparticles
keywords: Biocompatibility; High-abundance protein; Magnetic separation; Surface imprinting; Template immobilization