title: The Anti-Müllerian Hormone Profile is Linked with the In Vitro Embryo Production Capacity and Embryo Viability after Transfer but Cannot Predict Pregnancy Outcome.
abstract: The current study investigated the possibility of using the AMH concentration as a predictor of the ability of Korean Hanwoo cows to produce cumulus-oocyte complexes, embryos that survive after transfer as well as the pregnancy outcome of surrogates. Eight sessions of ovum pick-up (OPU) were performed with 19 donor cows at an interval of 3-4 days. Antral follicle count (AFC), oocyte quality and in vitro embryo development were recorded for each cow. Embryos produced from cows with different AMH profiles were transferred into recipients (n = 96). Cows in the high (≥0.25 ng/ml) and intermediate (0.1≥ to <0.25 ng/ml) AMH groups had a significantly higher AFC per OPU session (20.40 ± 1.36 and 16.91 ± 1.52, respectively; mean ± standard deviation) than cows in the low AMH group (<0.1 ng/ml; 12.19 ± 2.14). In addition, more cumulus-oocyte complexes per donor were recovered in the high (11.46 ± 1.22) and intermediate (7.38 ± 0.83) AMH groups than in the low AMH group (4.77 ± 0.44). The percentage of oocytes reached blastocyst stage was significantly higher in the intermediate (47.0%) and high (38.5%) AMH groups than in the low AMH group (32.3%). The number of embryos produced per cow was higher in the high (3.9 ± 0.2) and intermediate (6.9 ± 0.6) AMH groups than in the low AMH group (2.2 ± 0.3). The percentage of embryos that gave birth to viable calves when transferred into recipients was higher for those derived from cows in the intermediate AMH group (50.7%) than for those derived from cows in the low (35.7%) and high (36.4%) AMH groups. In conclusion, a single measurement of AMH concentration predicted the in vitro embryo production potential of donor Korean native cows before OPU and is linked with embryo viability after transfer into recipients.
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.|Department of Animal Production, Faculty of Agriculture Cairo University, Giza, Egypt.
authors.forename: N
authors.initials: N
authors.lastname: Ghanem
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: J I
authors.initials: JI
authors.lastname: Jin
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: S S
authors.initials: SS
authors.lastname: Kim
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: B H
authors.initials: BH
authors.lastname: Choi
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: K L
authors.initials: KL
authors.lastname: Lee
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: A N
authors.initials: AN
authors.lastname: Ha
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Song
authors.affiliation: Department of Animal Science, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju, Korea.|Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea.
authors.forename: I K
authors.initials: IK
authors.lastname: Kong
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood*; D002417:Cattle / Q000196:embryology*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary*; D011270:Pregnancy, Animal / Q000097:blood / Q000502:physiology
chemical_list: D054304:Anti-Mullerian Hormone