title: No current evidence for widespread dosage compensation in S. cerevisiae.
abstract: Previous studies of laboratory strains of budding yeast had shown that when gene copy number is altered experimentally, RNA levels generally scale accordingly. This is true when the copy number of individual genes or entire chromosomes is altered. In a recent study, Hose et al. (2015) reported that this tight correlation between gene copy number and RNA levels is not observed in recently isolated wild Saccharomyces cerevisiae variants. To understand the origins of this proposed difference in gene expression regulation between natural variants and laboratory strains of S. cerevisiae, we evaluated the karyotype and gene expression studies performed by Hose et al. on wild S. cerevisiae strains. In contrast to the results of Hose et al., our reexamination of their data revealed a tight correlation between gene copy number and gene expression. We conclude that widespread dosage compensation occurs neither in laboratory strains nor in natural variants of S. cerevisiae.
authors.affiliation: Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States.
authors.forename: Eduardo M
authors.initials: EM
authors.lastname: Torres
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Springer
authors.affiliation: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, United States.|Department of Biology, Massachusetts Institute of Technology, Cambridge, United States.|Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States.
authors.forename: Angelika
authors.initials: A
authors.lastname: Amon
mesh_terms: D000782:Aneuploidy; D018628:Gene Dosage; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D007621:Karyotyping; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger
keywords: S. cerevisiae; aneuploidy; cell biology; chromosomes; dosage compensation; gene expression; genes