title: Micro-RNA (miRNA) profile in Hodgkin lymphoma: association between clinical and pathological variables.
abstract: miRNAs are small RNAs and control the expression of protein-encoding genes. The aim of this study was to determine the association between miRNA profile and clinical variables including age, stage, B symptom, histopathologic subtype, response to treatment, disease-free survival (DFS) and overall survival (OS) in classical Hodgkin lymphoma (cHL). A total of 377 miRNAs were studied by qPCR in 32 cases with cHL, and results were compared with 60 samples taken from cases with reactive lymphadenopathy. Biogazelle qbasePLUS 2.0 software was used to analyze the results. miR-582-3p, miR-525-3p, miR-448, miR-512-3p, miR-642a-5p, miR-876-5p, miR-532-3p, miR-654-5p, miR-128, miR-145-5p, miR-15b-5p, miR-328 and miR-660-5p were found to be decreased in cHL compared with controls. In contrast, miR-34a-5p (2.626-fold), miR-146a-5p (4.32-fold), miR-93-5p (2.347-fold), miR-20a-5p (4.930-fold), miR-339-3p (4.948-fold), miR-324-3p (4.98-fold), miR-372 (7.038-fold), miR-127-3p (8.234-fold), miR-155-5p (4.947-fold), miR-320a (17.502-fold) and miR-370 (21.479-fold) (p < 0.05) were found to be increased in cHL. There was no difference in miRNA profile according to the age, sex, stage, response to treatment, DFS and OS. However, miR-889 was found to be increased in patients with B symptom and miR-127-3p was found to be increased in nodular sclerosing subtype. Some miRNAs increase and some decrease in cHL. However, there was no clinical association between clinical variables and with the majority of the miRNA profile studied in this study. miR-889 and miR-127-3p were related to B symptom and nodular sclerosis subtype, respectively. We need more studies evaluating miRNA profile and clinical outcome in Hodgkin Lymphoma.
authors.affiliation: Department of Medical Oncology, Faculty of Medicine, Cukurova University, Balcali, Adana, Turkey. sepay@cu.edu.tr.
authors.forename: Semra
authors.initials: S
authors.lastname: Paydas
authors.affiliation: Department of Pathology, Faculty of Medicine, Cukurova University, Adana, Turkey.
authors.forename: Arbil
authors.initials: A
authors.lastname: Acikalin
authors.affiliation: Department of Pathology, Faculty of Medicine, Cukurova University, Adana, Turkey.
authors.forename: Melek
authors.initials: M
authors.lastname: Ergin
authors.affiliation: TorosGen Biotechnology, Technoscope, Mersin University, Mersin, Turkey.
authors.forename: Hikmet
authors.initials: H
authors.lastname: Celik
authors.affiliation: TorosGen Biotechnology, Technoscope, Mersin University, Mersin, Turkey.
authors.forename: Basak
authors.initials: B
authors.lastname: Yavuz
authors.affiliation: Department of Biochemistry, Massachusetts General Hospital, Boston, MA, USA.
authors.forename: Kahraman
authors.initials: K
authors.lastname: Tanriverdi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006689:Hodgkin Disease / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D006801:Humans; D000072281:Lymphadenopathy / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged
chemical_list: C547276:MIRN127 microRNA, human; D035683:MicroRNAs
keywords: B symptom; Clinicopathological correlation; Hodgkin lymphoma; Prognosis; Subtype; Survival; miRNA; qPCR