title: Streamlined scanning for enhancer elements in Drosophila melanogaster.
abstract: Enhancer elements in most eukaryotic organisms are often positioned at a great distance away from the transcription start site of the gene they regulate. Complex three-dimensional chromatin organization and insulators usually guide and limit the range of an enhancer's regulatory activity to a specific genetic locus. Rigorous testing of an entire genomic locus is often required in order to uncover the complete set of cis-regulatory modules (CRMs) regulating a gene, especially those with complex and dynamic expression patterns. Here we report a fast and efficient method for enhancer element identification by scanning large genomic regions using transgenic reporter genes.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY.
authors.forename: Roumen
authors.initials: R
authors.lastname: Voutev
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Mann
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D016678:Genome / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D019076:Transgenes / Q000235:genetics
keywords: CRM; D. melanogaster; enhancer; reporter construct