title: Evaluation of the Fas/FasL signaling pathway in diabetic rat testis.
abstract: We investigated the role of the Fas/Fas ligand (FasL) signaling pathway in diabetic male infertility. Male rats were divided into two groups: a control group and a streptozotocin induced diabetic group. Thirty days after induction of diabetes, samples of testes were harvested and fixed in 10% formalin for light microscopy. Germ cell apoptosis was determined using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate in situ nick end-labeling (TUNEL) and immunostaining of caspase 8 and active caspase 3. We also investigated the expressions of Fas and FasL using immunohistochemistry. Streptozotocin-induced diabetes caused severe histopathological damage and increased apoptotic tubule and apoptotic cell indices, caspase 8 and caspase 3 expressions, and Fas and FasL-immunopositive cells in the rat testes. We suggest that the Fas/FasL signaling pathway may play a role in male infertility caused by diabetes.
authors.affiliation: a Trakya University, Faculty of Medicine , Department of Histology and Embryology , Edirne , Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Bayram
authors.affiliation: a Trakya University, Faculty of Medicine , Department of Histology and Embryology , Edirne , Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Kizilay
authors.affiliation: a Trakya University, Faculty of Medicine , Department of Histology and Embryology , Edirne , Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Topcu-Tarladacalisir
mesh_terms: D000818:Animals; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000503:physiopathology*; D053222:Fas Ligand Protein / Q000502:physiology*; D007150:Immunohistochemistry; D007248:Infertility, Male / Q000503:physiopathology; D008297:Male; D009929:Organ Size; D051381:Rats; D015398:Signal Transduction; D013737:Testis / Q000502:physiology*
chemical_list: D053222:Fas Ligand Protein
keywords: 3; Fas; FasL; caspase; caspase 8; diabetes mellitus; immunohistochemistry; rat; testis