title: Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells.
abstract: This data article describes cellular dynamics, such as migration speed and mobility of the cytoskeletal protein, of wild-type human fibroblast cells and cells with a modified adeno-associated virus integration site 1 (AAVS1) locus on human chromosome 19. Insertion of exogenous gene into the AAVS1 locus has been conducted in recent biological researches. Previously, our data showed that the AAVS1-modification changes cellular contractile force (Mizutani et al., 2015 [1]). To assess if this AAVS1-modification affects cell migration, we compared cellular migration speed and turnover of cytoskeletal protein in human fibroblasts and fibroblasts with a green fluorescent protein gene knocked-in at the AAVS1 locus in this data article. Cell nuclei were stained and changes in their position attributable to cell migration were analyzed. Fluorescence recovery was observed after photobleaching for the fluorescent protein-tagged myosin regulatory light chain. Data here are related to the research article "Transgene Integration into the Human AAVS1 Locus Enhances Myosin II-Dependent Contractile Force by Reducing Expression of Myosin Binding Subunit 85" [1].
authors.affiliation: Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Takeomi
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Haga
authors.affiliation: Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Kawabata
keywords: Cell migration; Genome editing; Mean square displacement; Myosin regulatory light chain