title: PiggyBac transposon vectors: the tools of the human gene encoding.
abstract: A transposon is a DNA segment, which is able to change its relative position within the entire genome of a cell. The piggyBac (PB) transposon is a movable genetic element that efficiently transposes between vectors and chromosomes through a "cut-and-paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeats (ITRs) sequences located on both ends of the transposon vector and eight efficiently moves the contents from its original positions and efficiently integrates them into TTAA chromosomal sites. PB has drawn much attention because of its transposition efficiency, safety and stability. Due to its priorities, PB can be used as a new genetic vehicle, a new tool for oncogene screening and a new method for gene therapy. PB has created a new outlook for human gene encoding.
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Enze
authors.initials: E
authors.lastname: Jiang
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Chen
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gu
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Anna Junjie
authors.initials: AJ
authors.lastname: Shangguan
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Tangfeng
authors.initials: T
authors.lastname: Lv
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Luo
authors.affiliation: 1 Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China ; 2 Department of Medical Oncology, Jinling Hospital, Nanjing University of Chinese Medicine, Nanjing 210002, China ; 3 Shanghai Medical College of Fudan University, Shanghai 20032, China ; 4 Weinberg College of Arts and Sciences at Northwestern University, Evanston, Illinois 60204, USA ; 5 Department of Respiratory Medicine, 6 Department of Cardiothoracic Surgery, Jinling Hospital, Nanjing 210002, China.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Yu
keywords: PiggyBac (PB); transgene; transposon