title: Diversification of Bromelioideae (Bromeliaceae) in the Brazilian Atlantic rainforest: A case study in Aechmea subgenus Ortgiesia.
abstract: Aechmea subgenus Ortgiesia comprises ca. 20 species distributed in Brazil, Argentina, Paraguay, and Uruguay, with a center of diversity in the Brazilian Atlantic rainforest. We examined interspecific relationships of Ortgiesia based on Amplified Fragment Length Polymorphisms (AFLP). Ninety-six accessions belonging to 14 species of Ortgiesia were sampled, and genotyped with 11 AFLP primer combinations. The neighbor joining (NJ) tree depicted two main genetic groups within Aechmea subgenus Ortgiesia, and four subgroups. The NJ tree showed short internal branches, indicating an overall shallow genetic divergence among Ortgiesia species as expected for the recently radiated subfamily Bromelioideae. Our results suggest that hybridization and/or incomplete lineage sorting may have hampered the reconstruction of interspecific relationships in Aechmea subgenus Ortgiesia. The mapping of petal color (yellow, blue, pink, or white), inflorescence type (simple or compound), and inflorescence shape (ellipsoid, subcylindric, cylindric, or pyramidal) against the NJ tree indicated that these characters are of limited taxonomic use in Aechmea subgenus Ortgiesia due to homoplasy. An analysis of the current distribution of Ortgiesia identified the southern region of the Brazilian Atlantic rainforest, between latitudes of 26° and 27°S, as the center of diversity for the subgenus.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Departamento de Genética, Avenida Bento Gonçalves 9500, PO Box 15053, 91501-970 Porto Alegre, RS, Brazil. Electronic address: marciagoetze@yahoo.com.br.
authors.forename: Márcia
authors.initials: M
authors.lastname: Goetze
authors.affiliation: Australian Tropical Herbarium, James Cook University, PO Box 6811, Cairns, QLD 4878, Australia; Centre for Tropical Biodiversity and Climate Change, James Cook University, Discovery Drive, Townsville, QLD 4814, Australia. Electronic address: katharina.schulte@jcu.edu.au.
authors.forename: Katharina
authors.initials: K
authors.lastname: Schulte
authors.affiliation: Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências, Departamento de Ecologia, Avenida 24A 1515, 13506-900 Rio Claro, SP, Brazil. Electronic address: clarissepalma@yahoo.com.br.
authors.forename: Clarisse
authors.initials: C
authors.lastname: Palma-Silva
authors.affiliation: Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Departamento de Genética, Avenida Bento Gonçalves 9500, PO Box 15053, 91501-970 Porto Alegre, RS, Brazil. Electronic address: milamzanella@gmail.com.
authors.forename: Camila M
authors.initials: CM
authors.lastname: Zanella
authors.affiliation: Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Departamento de Genética, Avenida Bento Gonçalves 9500, PO Box 15053, 91501-970 Porto Alegre, RS, Brazil. Electronic address: miriamvb@gmail.com.
authors.forename: Miriam V
authors.initials: MV
authors.lastname: Büttow
authors.affiliation: Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Departamento de Genética, Avenida Bento Gonçalves 9500, PO Box 15053, 91501-970 Porto Alegre, RS, Brazil. Electronic address: fecap@hotmail.com.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Capra
authors.affiliation: Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Departamento de Genética, Avenida Bento Gonçalves 9500, PO Box 15053, 91501-970 Porto Alegre, RS, Brazil. Electronic address: fernanda.bered@ufrgs.br.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Bered
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001118:Argentina; D001267:Atlantic Ocean; D001938:Brazil; D031229:Bromeliaceae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D006824:Hybridization, Genetic; D056626:Inflorescence / Q000145:classification / Q000235:genetics / Q000502:physiology; D010239:Paraguay; D010802:Phylogeny; D011110:Polymorphism, Genetic / Q000235:genetics*; D065947:Rainforest; D014583:Uruguay
keywords: AFLP; Aechmea; Biogeography; Core bromelioids; Interspecific relationships; Speciation