title: The ovarian response to standard gonadotropin stimulation is influenced by AMHRII genotypes.
abstract: The aim of the current study was to explore whether anti-Müllerian hormone receptor II (AMHRII) genetic variants influence the hormonal profile and the ovarian response to standard gonadotropin stimulation of women undergoing medically assisted reproduction. Three hundred in vitro fertilization or intracytoplasmic sperm injection patients constituted the study population, while 300 women with at least one spontaneous pregnancy participated as controls. The follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and AMH levels were determined at the third day of the menstrual cycle. AMHRII 10A > G (rs11170555), 1749C > T (rs2071558) and -482A > G (rs2002555) polymorphisms were genotyped. The follicle and oocyte numbers, the follicle size and the clinical pregnancies were recorded. Regarding the AMHRII 1749C > T polymorphism, 1749CT women presented with higher total follicle and small follicle numbers compared to 1749CC women (p = 0.04 and p = 0.01, respectively). Whereas, as concerns the -482A > G polymorphism, -482AG women were characterized by higher total follicle and small follicle numbers comparing with -482AA women (p = 0.07 and p = 0.004, respectively). Finally, -482AG women presented with increased FSH levels compared to -482AA women (p < 0.05). However, no associations of AMHRII gene polymorphisms with serum AMH levels or clinical pregnancy rates were observed. AMHRII 1749C > T and -482A > G genetic variants were associated with the ovarian response to standard gonadotropin stimulation, affecting mainly the follicular growth.
authors.affiliation: a Medical Genetics and Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Ioannina University Hospital , Ioannina , Greece.|b Laboratory of Medical Genetics of Human Reproduction , Medical School, Ioannina University , Ioannina , Greece.
authors.forename: Leandros
authors.initials: L
authors.lastname: Lazaros
authors.affiliation: b Laboratory of Medical Genetics of Human Reproduction , Medical School, Ioannina University , Ioannina , Greece.
authors.forename: Anthi
authors.initials: A
authors.lastname: Fotaki
authors.affiliation: a Medical Genetics and Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Ioannina University Hospital , Ioannina , Greece.
authors.forename: Christina
authors.initials: C
authors.lastname: Pamporaki
authors.affiliation: a Medical Genetics and Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Ioannina University Hospital , Ioannina , Greece.
authors.forename: Elissavet
authors.initials: E
authors.lastname: Hatzi
authors.affiliation: b Laboratory of Medical Genetics of Human Reproduction , Medical School, Ioannina University , Ioannina , Greece.
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Kitsou
authors.affiliation: a Medical Genetics and Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Ioannina University Hospital , Ioannina , Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Zikopoulos
authors.affiliation: c Department of Chemistry , Aristotle University of Thessaloniki , Thessaloniki , Greece , and.
authors.forename: Christina
authors.initials: C
authors.lastname: Virgiliou
authors.affiliation: d Department of Obstetrics and Gynecology , Ioannina State General Hospital G. Chatzikosta , Ioannina , Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Kosmas
authors.affiliation: b Laboratory of Medical Genetics of Human Reproduction , Medical School, Ioannina University , Ioannina , Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Bouba
authors.affiliation: a Medical Genetics and Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Ioannina University Hospital , Ioannina , Greece.
authors.forename: Theodoros
authors.initials: T
authors.lastname: Stefos
authors.affiliation: c Department of Chemistry , Aristotle University of Thessaloniki , Thessaloniki , Greece , and.
authors.forename: Georgios
authors.initials: G
authors.lastname: Theodoridis
authors.affiliation: a Medical Genetics and Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Ioannina University Hospital , Ioannina , Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Georgiou
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000097:blood*; D005260:Female; D005307:Fertilization in Vitro; D006062:Gonadotropins / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D009865:Oocytes / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods*; D011110:Polymorphism, Genetic; D018000:Receptors, Peptide / Q000235:genetics*; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D006062:Gonadotropins; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; C084621:anti-Mullerian hormone receptor; D054304:Anti-Mullerian Hormone
keywords: AMH receptor; IVF; follicles; oocytes; ovarian stimulation