title: CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis.
abstract: CURLY LEAF (CLF), a histone methyltransferase of Polycomb Repressive Complex 2 (PRC2) for trimethylation of histone H3 Lys 27 (H3K27me3), has been thought as a negative regulator controlling mainly postgermination growth in Arabidopsis (Arabidopsis thaliana). Approximately 14% to 29% of genic regions are decorated by H3K27me3 in the Arabidopsis genome; however, transcriptional repression activities of PRC2 on a majority of these regions remain unclear. Here, by analysis of transcriptome profiles, we found that approximately 11.6% genes in the Arabidopsis genome were repressed by CLF in various organs. Unexpectedly, approximately 54% of these genes were preferentially repressed in siliques. Further analyses of 118 transcriptome datasets uncovered a group of genes that was preferentially expressed and repressed by CLF in embryos at the mature-green stage. This observation suggests that CLF mediates a large-scale H3K27me3 programming/reprogramming event during embryonic development. Plants of clf-28 produced bigger and heavier seeds with higher oil content, larger oil bodies, and altered long-chain fatty acid composition compared with wild type. Around 46% of CLF-repressed genes were associated with H3K27me3 marks; moreover, we verified histone modification and transcriptional repression by CLF on regulatory genes. Our results suggest that CLF silences specific gene expression modules. Genes operating within a module have various molecular functions, but they cooperate to regulate a similar physiological function during embryo development.
authors.affiliation: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065;National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; andTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors.forename: Jun
authors.identifier: 0000-0003-1338-523X
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065;National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; andTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors.forename: Shulin
authors.identifier: 0000-0001-6872-9373
authors.initials: S
authors.lastname: Deng
authors.affiliation: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065;National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; andTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065;National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; andTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors.forename: Jian
authors.initials: J
authors.lastname: Ye
authors.affiliation: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065;National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; andTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Wu
authors.affiliation: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065;National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; andTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
authors.forename: Hai-Xi
authors.initials: HX
authors.lastname: Sun
authors.affiliation: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10065;National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; andTemasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore chua@mail.rockefeller.edu.
authors.forename: Nam-Hai
authors.initials: NH
authors.lastname: Chua
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D048429:Cell Size; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D063151:Polycomb Repressive Complex 2; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C104597:CURLY LEAF protein, Arabidopsis; D018398:Homeodomain Proteins; D008055:Lipids; C574170:PRC2 protein, Arabidopsis; D012097:Repressor Proteins; D063151:Polycomb Repressive Complex 2; D008239:Lysine