title: Two Canine Papillomaviruses Associated With Metastatic Squamous Cell Carcinoma in Two Related Basenji Dogs.
abstract: Papillomaviruses (PV) are associated with benign mucosal and cutaneous epithelial proliferations. In dogs, PV-associated pigmented plaques and papillomas can undergo malignant transformation, but this is rare, and most cases of canine squamous cell carcinoma do not arise from PV-induced precursor lesions. We describe herein the progression of pigmented plaques to invasive and metastatic squamous cell carcinoma associated with 2 canine papillomaviruses (CPV) in 2 related Basenji dogs. Immunohistochemistry for PV antigen revealed strong nuclear immunoreactivity within keratinocytes from pigmented plaques from both dogs, consistent with a productive viral infection. Polymerase chain reaction (PCR) using degenerate primers for the L1 gene revealed PV DNA sequences from 2 different CPVs. In situ hybridization for CPV revealed strong hybridization signals within the pigmented plaques and neoplastic squamous epithelial cells from both dogs. We report here progression of PV-associated pigmented plaques to metastatic squamous cell carcinoma within 2 Basenji dogs associated with 2 different CPVs.
authors.affiliation: North Carolina State University, Raleigh, NC, USA jaluff@ncsu.edu.
authors.forename: J
authors.initials: J
authors.lastname: Luff
authors.affiliation: Histopath Consulting, Worcester, MA, USA.
authors.forename: P
authors.initials: P
authors.lastname: Rowland
authors.affiliation: North Carolina State University, Raleigh, NC, USA.
authors.forename: M
authors.initials: M
authors.lastname: Mader
authors.affiliation: Animal Clinic of East Avenue, Rochester, NY, USA.
authors.forename: C
authors.initials: C
authors.lastname: Orr
authors.affiliation: Georgetown University Medical School, Washington, DC, USA.
authors.forename: H
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D017403:In Situ Hybridization / Q000662:veterinary; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012867:Skin / Q000473:pathology / Q000821:virology; D012878:Skin Neoplasms / Q000473:pathology / Q000662:veterinary* / Q000821:virology
keywords: PCR; canine; immunohistochemistry; in situ hybridization; papillomavirus; skin; squamous cell carcinoma