title: The effect of age on peripheral stem cell mobilization in healthy donors, single center experience.
abstract: OBJECTIVE
  Peripheral stem cell transplantation is used as a life-saving therapeutic option in hematological malignancies. As previously established, most hematological malignancies are seen in the elderly population. Therefore, possible HLA-identical sibling donors of elderly patients are generally of an advanced age. In this study, we aimed to evaluate the effect of old age on stem cell mobilization and quality in older adult healthy sibling donors.
  
  
  METHODS
  Between 2006 and 2014, we evaluated 38 healthy donors aged ≥55 years. The granulocyte-colony stimulating factor (G-CSF) analogs were used at a dose of 5 µg/kg/day and administered subcutaneously twice a day for five days. CD34+ cells were estimated in the peripheral blood before collection of the apheresis product. The National Marrow Donor Program selects healthy unrelated donors if they are younger than 60 years. Therefore, we compared the product quality in donors over the age of 60 to that in donors aged 60 years or less.
  
  
  RESULTS
  We collected sufficient products from all the donors with one to three apheresis procedures. No serious complication was detected in all donors. Reaching the target CD34+ cell count in one day were detected in 83% of younger and 79% of older donors (P = NS). Collected CD34+ cells x10e6/recipient body weight (kg) was same and 5.1 in the groups (P = NS). There were no correlation between the donor age and these parameters.
  
  
  CONCLUSIONS
  Healthy donor apheresis in older adults can be performed effectively and possible donors should be evaluated regardless of their age. J. Clin. Apheresis 32:16-20, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Hematology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozen
authors.affiliation: Department of Hematology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gunduz
authors.affiliation: Department of Hematology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Pervin
authors.initials: P
authors.lastname: Topcuoglu
authors.affiliation: Department of Hematology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Selami K
authors.initials: SK
authors.lastname: Toprak
authors.affiliation: Department of Hematology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Klara
authors.initials: K
authors.lastname: Dalva
authors.affiliation: Department of Hematology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Gunhan
authors.initials: G
authors.lastname: Gurman
authors.affiliation: Department of Hematology, Ankara University Faculty of Medicine, Ankara, Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Ilhan
mesh_terms: D000367:Age Factors; D000368:Aged; D018952:Antigens, CD34 / Q000097:blood; D001782:Blood Donors; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D007937:Leukapheresis / Q000379:methods; D008875:Middle Aged; D000072916:Peripheral Blood Stem Cells / Q000166:cytology
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor
keywords: apheresis; elderly donor; healthy donor; stem cell mobilization