title: Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities.
abstract: Since excessive reactive oxygen species (ROS) is known to be associated with aging and age-related diseases, strategies modulating ROS level and antioxidant defense systems may contribute to the delay of senescence. Here we show that the protein hydrolyzate from Angelica sinensis was capable of increasing oxidative survival of the model animal Caenorhabditis elegans intoxicated by paraquat. The hydrolyzate was then fractionated by ultrafiltration, and the antioxidant fraction (<3 kDa) was purified by gel filtration to obtain the antioxidant A. sinensis peptides (AsiPeps), which were mostly composed of peptides with <20 amino acid residues. Further studies demonstrate that AsiPeps were able to reduce the endogenous ROS level, increase the activities of the antioxidant enzymes superoxide dismutase and catalase, and decrease the content of the lipid peroxidation product malondialdehyde in nematodes treated with paraquat or undergoing senescence. AsiPeps were also shown to reduce age pigments accumulation and extend lifespan but did not affect the food-intake behavior of the nematodes. Taken together, our results demonstrate that A. sinensis peptides (AsiPeps) are able to delay aging process in C. elegans through antioxidant activities independent of dietary restriction.
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China; Research & Development Centre, Infinitus (China) Company Ltd., Guangzhou 510665, China; School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Yunxuan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Research & Development Centre, Infinitus (China) Company Ltd., Guangzhou 510665, China.
authors.forename: Chuixin
authors.initials: C
authors.lastname: Qin
authors.affiliation: Research & Development Centre, Infinitus (China) Company Ltd., Guangzhou 510665, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liang
authors.affiliation: Research & Development Centre, Infinitus (China) Company Ltd., Guangzhou 510665, China.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Mao
authors.affiliation: Shenzhen Key Laboratory of Microbiology and Gene Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Li
authors.affiliation: Shenzhen Key Laboratory of Microbiology and Gene Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, China.
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Weizhang
authors.initials: W
authors.lastname: Jia
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China; Research & Development Centre, Infinitus (China) Company Ltd., Guangzhou 510665, China; School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Li
authors.affiliation: Research & Development Centre, Infinitus (China) Company Ltd., Guangzhou 510665, China.
authors.forename: Chung Wah
authors.initials: CW
authors.lastname: Ma
authors.affiliation: Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000375:Aging / Q000187:drug effects / Q000502:physiology*; D000595:Amino Acid Sequence; D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D002417:Cattle; D002853:Chromatography, Liquid; D008315:Malondialdehyde / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010269:Paraquat / Q000633:toxicity; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010860:Pigments, Biological / Q000378:metabolism; D011492:Protein Hydrolysates / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D010455:Peptides; D010860:Pigments, Biological; D011492:Protein Hydrolysates; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D010269:Paraquat