title: Characterization of the Methylation Status of Pax7 and Myogenic Regulator Factors in Cell Myogenic Differentiation.
abstract: Epigenetic processes in the development of skeletal muscle have been appreciated for over a decade. DNA methylation is a major epigenetic modification important for regulating gene expression and suppressing spurious transcription. Up to now, the importance of epigenetic marks in the regulation of Pax7 and myogenic regulatory factors (MRFs) expression is far less explored. In the present study, semi-quantitative the real-time polymerase chain reaction (RT-PCR) analyses showed MyoD and Myf5 were expressed in activated and quiescent C2C12 cells. MyoG was expressed in a later stage of myogenesis. Pax7 was weakly expressed in differentiated C2C12 cells. To further understand the regulation of expression of these genes, the DNA methylation status of Pax7, MyoD, and Myf5 was determined by bisulfite sequencing PCR. During the C2C12 myoblasts fusion process, the changes of promoter and exon 1 methylation of Pax7, MyoD, and Myf5 genes were observed. In addition, an inverse relationship of low methylation and high expression was found. These results suggest that DNA methylation may be an important mechanism regulating Pax7 and MRFs transcription in cell myogenic differentiation.
authors.affiliation: Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chao
authors.affiliation: Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Xin-Li
authors.initials: XL
authors.lastname: Zheng
authors.affiliation: Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Sun
authors.affiliation: Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Liu
authors.affiliation: Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Huang
authors.affiliation: Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Zong-Xi
authors.initials: ZX
authors.lastname: Cao
authors.affiliation: College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chang-Yan
authors.initials: CY
authors.lastname: Deng
authors.affiliation: Institute of Animal Sciences and Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
keywords: C2C12; CpG Islands; DNA Methylation; Myogenic Regulatory Factors; Pax7