title: Dietary and lifestyle determinants of malondialdehyde DNA adducts in a representative sample of the Florence City population.
abstract: Malondialdehyde (MDA), a biomarker of lipid peroxidation and oxidative stress, is a mutagenic and carcinogenic compound that can react with DNA to form several types of DNA adducts including the deoxyguanosine adduct (M1dG). The aim of this cross-sectional study was to evaluate the association between individual dietary and lifestyle habits and M1dG levels, measured in peripheral leukocytes in a large representative sample of the general population of Florence City (Italy). Selected anthropometric measurements, detailed information on dietary and lifestyle habits and blood samples were available for 313 adults of the Florence City Sample enrolled in the frame of European Prospective Investigation into Cancer and nutrition (EPIC) study. A multivariate regression analysis adjusted for selected individual characteristics possibly related to M1dG levels (sex, age, BMI, smoke, physical activity level, education level, total caloric intake and a Mediterranean dietary score) was performed to estimate the association between these parameters and M1dG levels. M1dG levels were significantly higher in women (P = 0.014) and lower in moderately active or active subjects (P = 0.037).We also found a significant inverse association with the Modified Mediterranean dietary score (P for trend = 0.049), particularly evident for the highest categories of adherence. Our results indicate that M1dG levels can be modulated by selected individual characteristics such as gender, physical activity and a Mediterranean dietary pattern.
authors.forename: Calogero
authors.initials: C
authors.lastname: Saieva
authors.affiliation: Cancer Risk Factor Branch, Cancer Research and Prevention Institute (ISPO), Via Cosimo il Vecchio 2, 50139 Florence, Italy and.
authors.forename: Marco
authors.initials: M
authors.lastname: Peluso
authors.forename: Domenico
authors.initials: D
authors.lastname: Palli
authors.affiliation: Cancer Risk Factor Branch, Cancer Research and Prevention Institute (ISPO), Via Cosimo il Vecchio 2, 50139 Florence, Italy and.
authors.forename: Filippo
authors.initials: F
authors.lastname: Cellai
authors.forename: Marco
authors.initials: M
authors.lastname: Ceroti
authors.affiliation: Biomedical, Experimental and Clinical Sciences Department, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Selvi
authors.forename: Benedetta
authors.initials: B
authors.lastname: Bendinelli
authors.forename: Melania
authors.initials: M
authors.lastname: Assedi
authors.affiliation: Cancer Risk Factor Branch, Cancer Research and Prevention Institute (ISPO), Via Cosimo il Vecchio 2, 50139 Florence, Italy and.
authors.forename: Armelle
authors.initials: A
authors.lastname: Munnia
authors.forename: Giovanna
authors.initials: G
authors.lastname: Masala
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D018736:DNA Adducts / Q000032:analysis*; D004032:Diet; D005260:Female; D006801:Humans; D007558:Italy; D007962:Leukocytes / Q000378:metabolism* / Q000502:physiology; D008019:Life Style; D008297:Male; D008315:Malondialdehyde; D008875:Middle Aged; D011446:Prospective Studies; D012737:Sex Factors
chemical_list: D018736:DNA Adducts; D008315:Malondialdehyde