title: A Crosstalk Between K ras (Kirsten Rat Sarcoma Viral Oncogene Homologue) and Adherence Molecular Complex Leads to Disassociation of Cells-A Possible Contribution Towards Metastasis in Colorectal Cancer.
abstract: Constitutive activation of mutant K ras (Kirsten rat sarcoma viral oncogene homologue) and disassembly of E-cadherin-catenin complex (E-cadherin, α-catenin, β-catenin, and γ-catenin) play an important role in apoptosis, differentiation, and cell proliferation. In this study, the expression pattern of K ras and E-cadherin-catenin complex has been evaluated in normal and mutant colorectal cancer cell lines with an object to determine its impact on disassociation of cells from one another. We addressed the expression analysis of K ras with reference to its association with adherence molecules in two colorectal cancer cell lines, that is, Caco-2 (wild type K ras served as a control) and DLD1 (heterozygous mutation at codon 13) at message level by qRT-PCR and translational level by western blotting. Compared to the control Caco-2 cell lines, the K ras in DLD1 cell lines showed slightly higher values while α-catenin showed a slight lower (1.3-folds), β-catenin and E-cadherin showed significantly lower expression (4.2-fold decrease). It can be inferred that a possible cross talk exists between K ras and adherent junction mediated signalling. Mutation at codon 13 (G to D) leads to the overexpression of K ras and reduced expression of adherent junction complex resulting in metastasis. J. Cell. Biochem. 117: 2340-2345, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590, Pakistan.|Institute of Life Sciences, Swansea University Medical School, Singleton Park, Swansea, Wales, United Kingdom.
authors.forename: Bibi Nazia
authors.initials: BN
authors.lastname: Murtaza
authors.affiliation: Institute of Life Sciences, Swansea University Medical School, Singleton Park, Swansea, Wales, United Kingdom.
authors.forename: Shareen
authors.initials: S
authors.lastname: Doak
authors.affiliation: Institute of Life Sciences, Swansea University Medical School, Singleton Park, Swansea, Wales, United Kingdom.
authors.forename: Claire
authors.initials: C
authors.lastname: Morgan
authors.affiliation: Department of Biochemistry, King Abdul Aziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Muhammad Shahid
authors.initials: MS
authors.lastname: Nadeem
authors.affiliation: Department of Zoology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Kingdom of Saudi Arabia.
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Al-Ghanim
authors.affiliation: School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, 54590, Pakistan.|Department of Zoology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Kingdom of Saudi Arabia.
authors.forename: Abdul Rauf
authors.initials: AR
authors.lastname: Shakoori
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism*; D051177:Catenins / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000556:secondary*; D006801:Humans; D007124:Immunoenzyme Techniques; D009362:Neoplasm Metastasis; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D015820:Cadherins; D051177:Catenins; C117307:KRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)
keywords: COLORECTAL CANCER; E-CADHERIN (CDH1); GENE EXPRESSION; KIRSTEIN RAS SARCOMA VIRAL ONCOGENE HOMOLOGUE; METASTASIS; α-CATENIN (CTNNA1); β-CATENIN