title: Medium engineering for enhanced production of undecylprodigiosin antibiotic in Streptomyces coelicolor using oil palm biomass hydrolysate as a carbon source.
abstract: In this study, a biosugar obtained from empty fruit bunch (EFB) of oil palm by hot water treatment and subsequent enzymatic saccharification was used for undecylprodigiosin production, using Streptomyces coelicolor. Furfural is a major inhibitor present in EFB hydrolysate (EFBH), having a minimum inhibitory concentration (MIC) of 1.9mM, and it reduces utilization of glucose (27%), xylose (59%), inhibits mycelium formation, and affects antibiotic production. Interestingly, furfural was found to be a good activator of undecylprodigiosin production in S. coelicolor, which enhanced undecylprodigiosin production by up to 52%. Optimization by mixture analysis resulted in a synthetic medium containing glucose:furfural:ACN:DMSO (1%, 2mM, 0.2% and 0.3%, respectively). Finally, S. coelicolor was cultured in a fermenter in minimal medium with EFBH as a carbon source and addition of the components described above. This yielded 4.2μg/mgdcw undecylprodigiosin, which was 3.2-fold higher compared to that in un-optimized medium.
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Shashi Kant
authors.initials: SK
authors.lastname: Bhatia
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Bo-Rahm
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Sathiyanarayanan
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Hun-Seok
authors.initials: HS
authors.lastname: Song
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Junyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jeon
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Food Ingredients Center, Foods R&D, CheilJedang, Guro-dong, Guro-Gu, Seoul 152-051, South Korea.
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.affiliation: Center for Industrial Chemical Biotechnology, Ulsan Chemical R&BD Division, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon 305-600, South Korea.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Yu
authors.affiliation: Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong City 339-701, South Korea.
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, South Korea; Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong City 339-701, South Korea; Microbial Carbohydrate Resource Bank, Konkuk University, Seoul 143-701, South Korea; Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul 143-701, South Korea. Electronic address: seokor@konkuk.ac.kr.
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000975:Antioxidants / Q000494:pharmacology; D028023:Arecaceae / Q000737:chemistry*; D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000494:pharmacology*; D005227:Fatty Acids / Q000096:biosynthesis; D005638:Fruit / Q000737:chemistry; D005662:Furaldehyde / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D008962:Models, Theoretical; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D015203:Reproducibility of Results; D012997:Solvents; D048372:Streptomyces coelicolor / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014867:Water; D014994:Xylose / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005227:Fatty Acids; D012997:Solvents; D014867:Water; C047347:undecylprodigiosin; D002244:Carbon; D014994:Xylose; D005662:Furaldehyde; D005947:Glucose; D011353:Prodigiosin
keywords: Antibiotic; Biosugar; Furfural; Streptomyces coelicolor; Undecylprodigiosin