title: Surface free energy predominates in cell adhesion to hydroxyapatite through wettability.
abstract: The initial adhesion of cells to biomaterials is critical in the regulation of subsequent cell behaviors. The purpose of this study was to investigate a mechanism through which the surface wettability of biomaterials can be improved and determine the effects of biomaterial surface characteristics on cellular behaviors. We investigated the surface characteristics of various types of hydroxyapatite after sintering in different atmospheres and examined the effects of various surface characteristics on cell adhesion to study cell-biomaterial interactions. Sintering atmosphere affects the polarization capacity of hydroxyapatite by changing hydroxide ion content and grain size. Compared with hydroxyapatite sintered in air, hydroxyapatite sintered in saturated water vapor had a higher polarization capacity that increased surface free energy and improved wettability, which in turn accelerated cell adhesion. We determined the optimal conditions of hydroxyapatite polarization for the improvement of surface wettability and acceleration of cell adhesion.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan. Electronic address: miho.bcr@tmd.ac.jp.
authors.forename: Miho
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Hori
authors.affiliation: Department of Materials and Applied Chemistry, Nihon University, 1-5-1 Kanda-Surugadai, Chiyoda, Tokyo 1018308, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ando
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan; Department of Materials and Applied Chemistry, Nihon University, 1-5-1 Kanda-Surugadai, Chiyoda, Tokyo 1018308, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Namba
authors.affiliation: Department of Materials and Applied Chemistry, Nihon University, 1-5-1 Kanda-Surugadai, Chiyoda, Tokyo 1018308, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Toyama
authors.affiliation: Department of Materials and Applied Chemistry, Nihon University, 1-5-1 Kanda-Surugadai, Chiyoda, Tokyo 1018308, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Nishimiya
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 1010062, Japan.
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature; D013816:Thermodynamics; D017155:Wettability; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite
keywords: Cell adhesion; Hydroxyapatite; Surface characteristics; Surface free energy; Wettability