title: Superelastic, superabsorbent and 3D nanofiber-assembled scaffold for tissue engineering.
abstract: Fabrication of 3D scaffold to mimic the nanofibrous structure of the nature extracellular matrix (ECM) with appropriate mechanical properties and excellent biocompatibility, remain an important technical challenge in tissue engineering. The present study reports the strategy to fabricate a 3D nanofibrous scaffold with similar structure to collagen in ECM by combining electrospinning and freeze-drying technique. With the technique reported here, a nanofibrous structure scaffold with hydrophilic and superabsorbent properties can be readily prepared by Gelatin and Polylactic acid (PLA). In wet state the scaffold also shows a super-elastic property, which could bear a compressive strain as high as 80% and recovers its original shape afterwards. Moreover, after 6 days of culture, L-929 cells grow, proliferate and infiltrated into the scaffold. The results suggest that this 3D nanofibrous scaffold would be promising for varied field of tissue engineering application.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Changzheng Hospital affiliated with Second Military Medical University, 415 Fengyang Road, Shanghai 200003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science, Donghua University, Shanghai 201620, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Faculty of Sciences, Engineering and Technology, Hawthorn, Victoria 3122, Australia.
authors.forename: Yosry
authors.initials: Y
authors.lastname: Morsi
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.
authors.forename: Hany
authors.initials: H
authors.lastname: -Ei-Hamshary
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Salem S
authors.initials: SS
authors.lastname: Al-Deyab
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Shandong International Biotechnology Park Development Co., Ltd., China. Electronic address: xmm@dhu.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry* / Q000494:pharmacology; D018929:Cell Culture Techniques; D002460:Cell Line; D003094:Collagen / Q000737:chemistry; D004548:Elasticity; D055664:Electrochemical Techniques; D005109:Extracellular Matrix / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005612:Freeze Drying; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C033616:poly(lactide); D005780:Gelatin; D003094:Collagen
keywords: Electrospinning; Nanofiber; Scaffold; Superelastic; Tissue engineering