title: Genotyping of Enterocytozoon bieneusi (Microsporidia) isolated from various birds in China.
abstract: Enterocytozoon bieneusi is a common opportunistic pathogen causing diarrhea in humans and animals. However, epidemiological data on E. bieneusi infections in birds are relatively scare worldwide, especially in China. To understand the prevalence and genetic diversity of E. bieneusi in birds and to assess the zoonotic potential of bird-derived E. bieneusi isolates, 194 fecal specimens from Gruidae, Anatidae and Columbidae in Heilongjiang Province, China, were analyzed by PCR and sequencing of the single internal transcribed spacer region of the rRNA gene. The average prevalence of E. bieneusi was 22.2%, with 12.5% for Gruidae, 15.9% for Anatidae and 44.0% for Columbidae. Altogether seven genotypes of E. bieneusi were identified, including four known genotypes-Peru6 (n=29), BEB6 (n=5), D (n=3) and EbpA (n=1)-and three novel genotypes named CHN-B1 (n=1), CHN-B2 (n=3) and CHN-B3 (n=1). All the known genotypes obtained here were previously detected in humans. All the novel genotypes were clustered into the zoonotic group 1 in phylogenetic analysis. The results indicate that these birds may play a potential role in the transmission of E. bieneusi to humans.
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Laboratory Medicine, Xiangya Medical School, Central South University, Changsha, Hunan 410008, China.
authors.forename: Siyang
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Ziyin
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Yichi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Weizhe
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Fengkun
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China. Electronic address: liuaiqin1128@126.com.
authors.forename: Aiqin
authors.initials: A
authors.lastname: Liu
mesh_terms: D000818:Animals; D001717:Birds / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D014644:Genetic Variation; D016681:Genome, Fungal; D005838:Genotype; D006801:Humans; D016814:Microsporidia / Q000235:genetics* / Q000302:isolation & purification; D009181:Mycoses / Q000382:microbiology*; D017422:Sequence Analysis, DNA / Q000379:methods*; D015047:Zoonoses / Q000382:microbiology
keywords: Birds; Enterocytozoon bieneusi; Gene diversity; Genotyping; ITS region; Phylogeny