title: Mineralocorticoid Receptor Deficiency in Macrophages Inhibits Neointimal Hyperplasia and Suppresses Macrophage Inflammation Through SGK1-AP1/NF-κB Pathways.
abstract: Restenosis after percutaneous coronary intervention remains to be a serious medical problem. Although mineralocorticoid receptor (MR) has been implicated as a potential target for treating restenosis, the cellular and molecular mechanisms are largely unknown. This study aims to explore the functions of macrophage MR in neointimal hyperplasia and to delineate the molecular mechanisms.
  
  
  
  Myeloid MR knockout (MMRKO) mice and controls were subjected to femoral artery injury. MMRKO reduced intima area and intima/media ratio, Ki67- and BrdU-positive vascular smooth muscle cells, expression of proinflammatory molecules, and macrophage accumulation in injured arteries. MMRKO macrophages migrated less in culture. MMRKO decreased Ki67- and BrdU-positive macrophages in injured arteries. MMRKO macrophages were less Ki67-positive in culture. Conditioned media from MMRKO macrophages induced less migration, Ki67 positivity, and proinflammatory gene expression of vascular smooth muscle cells. After lipopolysaccharide treatment, MMRKO macrophages had decreased p-cFos and p-cJun compared with control macrophages, suggesting suppressed activation of activator protein-1 (AP1). Nuclear factor-κB (NF-κB) pathway was also inhibited by MMRKO, manifested by decreased p-IκB kinase-β and p-IκBα, increased IκBα expression, decreased nuclear translocation of p65 and p50, as welll as decreased phosphorylation and expression of p65. Finally, overexpression of serum-and-glucocorticoid-inducible-kinase-1 (SGK1) attenuated the effects of MR deficiency in macrophages.
  
  
  
  Selective deletion of MR in myeloid cells limits macrophage accumulation and vascular inflammation and, therefore, inhibits neointimal hyperplasia and vascular remodeling. Mechanistically, MR deficiency suppresses migration and proliferation of macrophages and leads to less vascular smooth muscle cell activation. At the molecular level, MR deficiency suppresses macrophage inflammatory response via SGK1-AP1/NF-κB pathways.
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Sun
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Zhu-Xia
authors.initials: ZX
authors.lastname: Shen
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Wu-Chang
authors.initials: WC
authors.lastname: Zhang
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Tang-Jun
authors.initials: TJ
authors.lastname: Ai
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Lin-Juan
authors.initials: LJ
authors.lastname: Du
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Yu-Yao
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Yao
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Shuyang
authors.initials: S
authors.lastname: Sun
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Aniko
authors.initials: A
authors.lastname: Naray-Fejes-Toth
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Geza
authors.initials: G
authors.lastname: Fejes-Toth
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Mao
authors.initials: M
authors.lastname: Chen
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liu
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Feifan
authors.initials: F
authors.lastname: Guo
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.).
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: From the Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Shanghai, China (J.-Y.S., C.L., Z.-X.S., W.-C.Z., T.-J.A., L.-J.D., Y.-Y.Z., G.-F.Y., Y.L., B.Z., Y.Y., F.G., S.-Z.D.); Shanghai Key Laboratory of Stomatology, Department of Oral and Maxillofacial-Head Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (S.S.); Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth, Lebanon, NH (A.N.-F.-T., G.F.-T.); Department of Cardiology (Y.P., M.C.) and Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center (X.L.), West China Hospital, Sichuan University, Chengdu, China; Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China (J.T.); and Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing Anzhen Hospital of the Capital Medical University, Beijing, China (J.D.). szduan@sibs.ac.cn.
authors.forename: Sheng-Zhong
authors.initials: SZ
authors.lastname: Duan
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques; D004195:Disease Models, Animal; D005263:Femoral Artery / Q000201:enzymology / Q000293:injuries / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D006965:Hyperplasia; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D007249:Inflammation / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D008264:Macrophages / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000201:enzymology* / Q000293:injuries / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000201:enzymology* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism*; D058426:Neointima; D019899:Paracrine Communication; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D000067996:RAW 264.7 Cells; D034622:RNA Interference; D018161:Receptors, Mineralocorticoid / Q000172:deficiency* / Q000235:genetics; D015398:Signal Transduction; D013997:Time Factors; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014162:Transfection; D066253:Vascular Remodeling; D057772:Vascular System Injuries / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000517:prevention & control; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D017874:Immediate-Early Proteins; D016328:NF-kappa B; D017346:Protein Serine-Threonine Kinases; D018161:Receptors, Mineralocorticoid; D018808:Transcription Factor AP-1; D000099184:Serum-Glucocorticoid Regulated Kinases
keywords: femoral artery; hyperplasia; macrophage; mineralocorticoid receptor; myeloid cells