title: The immune responses and antioxidant status of Portunus trituberculatus individuals with different body weights.
abstract: Vibrio alginolyticus is a virulent pathogen that affects crab aquacultures. In the present study, the immune responses and antioxidant status of big and small (based on body weight and size) 80-, 100- and 120-day-old specimens of Portunus trituberculatus, challenged for 72 h with Vibrio alginolyticus, were studied. The total hemocyte count (THC), and phagocytic, prophenoloxidase and phenoloxidase activities, of the big individuals (BIs) were higher than those of the small individuals (SIs) (P < 0.05). The antioxidant status of the organisms showed a similar pattern: superoxide dismutase (SOD) activity and glutathione/oxidized glutathione (GSH/GSSG) in the cell-free hemolymph and hepatopancreases of the BIs were higher than in the SIs (P < 0.05). There were no significant differences in α2-macroglobulin (α2-M), antibacterial and bacteriolytic activities in the cell-free hemolymph, or glutathione peroxidase activity in the cell-free hemolymph or hepatopancreas between the BIs and SIs. The α2-M and crustin gene expression levels in the hemocytes, and SOD expression in the hemocytes and hepatopancreas, were also significantly higher in the BIs. The results suggest that, compared with the SIs, the BIs possessed a higher resistance to V. alginolyticus infection.
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
authors.forename: Xianyun
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China; College of Fisheries and Life Science, Dalian Ocean University, Dalian, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
authors.forename: Baoquan
authors.initials: B
authors.lastname: Gao
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China. Electronic address: lijian@ysfri.ac.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism; D001772:Blood Cell Count; D001835:Body Weight; D003386:Brachyura / Q000502:physiology*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004156:Catechol Oxidase / Q000235:genetics / Q000378:metabolism; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006434:Hemocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D006458:Hemolymph / Q000276:immunology; D043143:Hepatopancreas / Q000378:metabolism; D010587:Phagocytosis; D012333:RNA, Messenger / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014735:Vibrio Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary; D044149:Vibrio alginolyticus; D000511:alpha-Macroglobulins / Q000235:genetics
chemical_list: D060829:Arthropod Proteins; D004792:Enzyme Precursors; D012333:RNA, Messenger; D000511:alpha-Macroglobulins; C038451:pro-phenoloxidase; D004156:Catechol Oxidase; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D019803:Glutathione Disulfide
keywords: Antioxidant status; Body weight; Immune response; Portunus trituberculatus; Vibrio alginolyticus