title: [Effect of lactic acid bacteria isolated from Tibetan Plateau on silage fermentation quality of Elms nutans].
abstract: OBJECTIVE
  In order to detect the effect of lactic acid bacteria isolated from Tibetan Plateau on silage fermentation quality of Elms nutans.
  
  
  METHODS
  We used 3 isolated lactic acid bacteria with better growth at low temperatures of 10 and 15 degrees C at ensiling of Elymus nutans. Subsequently, effects of the selected lactic acid bacteria on fermentation profiles of Elymus nutans silages stored at 15 and 25 degrees C were evaluated by using the same species of commercial inoculants as the control.
  
  
  RESULTS
  PP-6 isolated from Tibetan Plateau could ferment raffinose, lactose, sorbitol, melibiose and sucrose, and LS-5 could ferment cottonseed sugar, laetrile, rhamnose, lactose, sorbitol, xylose, arabinose, melibiose and sucrose, but the same species of commercial strains could not use these sugars. Inoculation of these three strains into Elymus nutans at 15 and 25 degrees C ensiled for 50 d, we found that LS-5 significantly reduced silage pH, propionic acid concentration and ratio of ammonia nitrogen/total nitrogen at 15 degrees C (P < 0.05), salvaged more water-soluble carbohydrate and crude protein; Application of LP-2 and PP-6 as a combined inoculant to Elymus nutans significantly improved lactic acid concentration (P < 0.05), resulting in a lower ratio of ammonia nitrogen/total nitrogen, saved more crude protein and significantly reduced neutral detergent fiber content (P < 0.05) as compared with the commercial strains.
  
  
  CONCLUSIONS
  The three isolated strains can improve silage quality of Elymus nutans growing on the Qinghai-Tibetan Plateau at low temperature, but these strains have no obvious advantages at 25 degrees C in comparison with the commercial inoculants.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.forename: Wencan
authors.initials: W
authors.lastname: Ke
authors.forename: Peixin
authors.initials: P
authors.lastname: Jing
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Yingwen
authors.initials: Y
authors.lastname: Yu
authors.forename: Xusheng
authors.initials: X
authors.lastname: Guo
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D031749:Elymus / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D005285:Fermentation; D019344:Lactic Acid / Q000378:metabolism; D007777:Lactobacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012820:Silage / Q000032:analysis / Q000382:microbiology*; D018609:Tibet
chemical_list: D019344:Lactic Acid