title: Association between p53-binding protein 1 expression and genomic instability in oncocytic follicular adenoma of the thyroid.
abstract: Oncocytic follicular adenomas (FAs) of the thyroid are neoplasms of follicular cell origin that are predominantly composed of large polygonal cells with eosinophilic and granular cytoplasm. However, the pathological characteristics of these tumors are largely unexplored. Both the initiation and progression of cancer can be caused by an accumulation of genetic mutations that can induce genomic instability. Thus, the aim of this study was to evaluate the extent of genomic instability in oncocytic FA. As the presence of p53-binding protein 1 (53BP1) in nuclear foci has been found to reflect DNA double-strand breaks that are triggered by various stresses, the immunofluorescence expression pattern of 53BP-1 was assessed in oncocytic and conventional FA. The association with the degree of DNA copy number aberration (CNA) was also evaluated using array-based comparative genomic hybridization. Data from this study demonstrated increased 53BP1 expression (i.e., "unstable" expression) in nuclear foci of oncocytic FA and a higher incidence of CNAs compared with conventional FA. There was also a particular focus on the amplification of chromosome 1p36 in oncocytic FA, which includes the locus for Tumor protein 73, a member of the p53 family implicated as a factor in the development of malignancies. Further evaluations revealed that unstable 53BP1 expression had a significant positive correlation with the levels of expression of Tumor protein 73. These data suggest a higher level of genomic instability in oncocytic FA compared with conventional FA, and a possible relationship between oncocytic FA and abnormal amplification of Tumor protein 73.
authors.affiliation: Department of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki 852-8523, Japan.
authors.forename: Zhanna
authors.initials: Z
authors.lastname: Mussazhanova
authors.forename: Yuko
authors.initials: Y
authors.lastname: Akazawa
authors.forename: Katsuya
authors.initials: K
authors.lastname: Matsuda
authors.forename: Kazuko
authors.initials: K
authors.lastname: Shichijo
authors.forename: Shiro
authors.initials: S
authors.lastname: Miura
authors.forename: Ryota
authors.initials: R
authors.lastname: Otsubo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Oikawa
authors.forename: Koh-Ichiro
authors.initials: K
authors.lastname: Yoshiura
authors.forename: Norisato
authors.initials: N
authors.lastname: Mitsutake
authors.forename: Tatiana
authors.initials: T
authors.lastname: Rogounovitch
authors.forename: Vladimir
authors.initials: V
authors.lastname: Saenko
authors.forename: Zhanna
authors.initials: Z
authors.lastname: Kozykenova
authors.forename: Bekbolat
authors.initials: B
authors.lastname: Zhetpisbaev
authors.forename: Dariya
authors.initials: D
authors.lastname: Shabdarbaeva
authors.forename: Nurlan
authors.initials: N
authors.lastname: Sayakenov
authors.forename: Bakanay
authors.initials: B
authors.lastname: Amantayev
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Kondo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ito
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakashima
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000150:complications / Q000235:genetics* / Q000473:pathology; D000236:Adenoma / Q000150:complications / Q000235:genetics* / Q000473:pathology; D018249:Adenoma, Oxyphilic / Q000150:complications / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D042822:Genomic Instability / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D013964:Thyroid Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology; D000071857:Tumor Suppressor p53-Binding Protein 1 / Q000235:genetics*
chemical_list: C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1