title: Role of oxidative stress and low-grade inflammation in letrozole-induced polycystic ovary syndrome in the rat.
abstract: The aims of the current study were to examine the effects of temporal changes in oxidative stress (OS) and low-grade inflammation in letrozole-treated rats and to correlate these changes with the development of polycystic ovary syndrome (PCOS)-like features. Rats were treated with letrozole for 7, 15 and 21 days to induce PCOS. On day 7 of the treatment, a significant increase in serum testosterone and high sensitive C-reactive protein (hsCRP), the low-grade inflammatory marker, was found in the letrozole treated rats compared to control rats. Moreover, a decreased immunoexpression of insulin receptor coincided with increased body weight. The strong correlation between the levels of hsCRP and lipid peroxidation (LPO) suggests simultaneous development of low-grade inflammation and OS in response to hyperandrogenism, and the role of OS in a formation of cystic follicles in the letrozole animal PCOS model. Therefore, the results of the present study suggest that OS and low-grade inflammation (hsCRP) are the major causes of PCOS induction in this model.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Ayurveda, Banaras Hindu University, Varanasi, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Pandey
authors.affiliation: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Anusha
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, India.
authors.forename: Amitabh
authors.initials: A
authors.lastname: Krishna
authors.affiliation: Department of Gynaecology, Banaras Hindu University, Varanasi, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Pandey
authors.affiliation: Department of Medicinal Chemistry, Faculty of Ayurveda, Banaras Hindu University, Varanasi, India. Electronic address: Yamini30@gmail.com.
authors.forename: Yamini Bhusan
authors.initials: YB
authors.lastname: Tripathi
mesh_terms: D000818:Animals; D047072:Aromatase Inhibitors / Q000633:toxicity*; D002097:C-Reactive Protein; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000473:pathology*; D000077289:Letrozole; D015227:Lipid Peroxidation; D009570:Nitriles / Q000633:toxicity*; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects*; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism; D013482:Superoxide Dismutase; D013739:Testosterone; D014230:Triazoles / Q000633:toxicity*
chemical_list: D047072:Aromatase Inhibitors; D009570:Nitriles; D014230:Triazoles; D013739:Testosterone; D000077289:Letrozole; D002097:C-Reactive Protein; D013482:Superoxide Dismutase; D011972:Receptor, Insulin
keywords: Inflammation; Insulin resistance; Oxidative stress; Polycystic ovary syndrome