title: Exogenous melatonin improves corn (Zea mays L.) embryo proteome in seeds subjected to chilling stress.
abstract: Melatonin (MEL; N-acetyl-5-methoxytryptamine) plays an important role in plant stress defense. Various plant species rich in this indoleamine have shown a higher capacity for stress tolerance. Moreover, it has great potential for plant biostimulation, is biodegradable and non-toxic for the environment. All this indicates that our concept of seed enrichment with exogenous MEL is justified. This work concerns the effects of corn (Zea mays L.) seed pre-sowing treatments supplemented with MEL. Non-treated seeds (nt), and those hydroprimed with water (H) or with MEL solutions 50 and 500 μM (HMel50, HMel500) were compared. Positive effects of seed priming are particularly apparent during germination under suboptimal conditions. The impact of MEL applied by priming on seed protein profiles during imbibition/germination at low temperature has not been investigated to date. In order to identify changes in the corn seed proteome after applying hydropriming techniques, purified protein extracts of chilling stressed seed embryos (14 days, 5°C) were separated by two-dimensional electrophoresis. Then proteome maps were graphically and statistically compared and selected protein spots were qualitatively analyzed using mass spectrometry techniques and identified. This study aimed to analyze the priming-induced changes in maize embryo proteome and at identifying priming-associated and MEL-associated proteins in maize seeds subjected to chilling. We attempt to explain how MEL expands plant capacity for stress tolerance.
authors.affiliation: Department of Ecophysiology and Plant Development, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland. Electronic address: izka.kolo@gmail.com.
authors.forename: Izabela
authors.initials: I
authors.lastname: Kołodziejczyk
authors.affiliation: Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland. Electronic address: dzika@biol.uni.lodz.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Dzitko
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland. Electronic address: rszewcz@biol.uni.lodz.pl.
authors.forename: Rafał
authors.initials: R
authors.lastname: Szewczyk
authors.affiliation: Department of Ecophysiology and Plant Development, Faculty of Biology and Environmental Protection, University of Lodz, 12/16 Banacha Str., 90-237 Lodz, Poland. Electronic address: posmyk@biol.uni.lodz.pl.
authors.forename: Małgorzata M
authors.initials: MM
authors.lastname: Posmyk
mesh_terms: D003080:Cold Temperature; D018525:Germination / Q000187:drug effects; D008550:Melatonin / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D020543:Proteome; D036226:Seedlings / Q000187:drug effects / Q000502:physiology; D012639:Seeds / Q000187:drug effects / Q000502:physiology; D013312:Stress, Physiological; D003313:Zea mays / Q000187:drug effects / Q000502:physiology*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D008550:Melatonin
keywords: Antioxidant; Chilling stress; Melatonin; Proteome analysis; Seed priming; Zea mays