title: Occurrence of aminoglycoside-modifying enzymes genes (aac(6')-I and ant(2″)-I) in clinical isolates of Pseudomonas aeruginosa from Southwest Nigeria.
abstract: BACKGROUND
  Enzymatic modification of aminoglycosides is the primary mechanism of resistance by Pseudomonas aeruginosa.
  
  
  OBJECTIVE
  We investigated the occurrence and mechanism of aminoglycosides resistance in P. aeruginosa isolates from hospitals in SouthWest Nigeria.
  
  
  METHODS
  A total of 54 consecutive, non-duplicate clinical isolates of P. aeruginosa were studied for the presence of aminoglycosides -modifying enzymes (AMEs) by PCR amplification and sequencing of genes encoding AMEs.
  
  
  CONCLUSIONS
  Two types of AME genes [aac (6') - I and ant (2″) - I] were found in 12 isolates out of 54. Seven strains harboured one or more types of enzymes of which aac (6') - I was the most frequently found gene (10/54 isolates, 18.5%). None of the isolates investigated in this study were positive for aph, aac (3) and aac (6″) - II genes. Prevalence of P. aeruginosa producing AME genes in this study may suggest aminoglycosides use in Nigeria. This study highlights need for functional antimicrobial surveillance system in Nigeria.
authors.affiliation: Department of Microbiology University of Lagos, Akoka Lagos; Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria; Environmental Microbiology Section, Indian Institute of Toxicology Research, Lucknow, India.
authors.forename: Bamidele Tolulope
authors.initials: BT
authors.lastname: Odumosu
authors.affiliation: Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.
authors.forename: Bolanle A
authors.initials: BA
authors.lastname: Adeniyi
authors.affiliation: Environmental Microbiology Section, Indian Institute of Toxicology Research, Lucknow, India.
authors.forename: Ram
authors.initials: R
authors.lastname: Chandra
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000617:Aminoglycosides / Q000378:metabolism / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D009549:Nigeria; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase
keywords: Aminoglycoside-modifying enzymes; Pseudomonas aeruginosa; antibiotics resistance