title: Comparison of the bacterial composition of two commercial composts with different physicochemical, stability and maturity properties.
abstract: Previously, two municipal solid waste commercial composts (MSW1 and MSW2) were characterized. Although sharing the same type of raw material, most of their physicochemical, stability and maturity properties differed. The present study aimed to characterize them at a microbiological level, and to infer on possible relationships between the composts properties and the structure of their bacterial communities. Both the 16S rRNA gene-based PCR-DGGE profiling and 454-pyrosequencing technology showed that the structure of the bacterial communities of these composts was distinct. The bacterial community of MSW1 was more diverse than that of MSW2. Multivariate analyses revealed that the high electrical conductivity, Cu content as well as the low phytotoxity of compost MSW1, when compared to MSW2, contributed most to shape its bacterial community structure. Indeed, high abundance of halophilic (Halomonadaceae and Brevibacteriaceae) and metal resistant organisms (Brevibacteriaceae and Bacillaceae) were found in MSW1. In addition, Pseudonocardiaceae, Streptomycetaceae, Bacillaceae, and Brevibacteriaceae may have contributed to the high humic-like acids content and low phytotoxicity of MSW1. In contrast, the high organic matter content and the high density of the cultivable fungi population were the parameters most correlated with the structure of the bacterial community of compost MSW2, dominated by Corynebacteriaceae and mainly Aerococcaceae, taxonomic groups not commonly found in composts.
authors.affiliation: CI&DEST, Escola Superior de Tecnologia e Gestão de Viseu, Instituto Politécnico de Viseu, 3504-510 Viseu, Portugal; LEPABE, Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; LEAF, Centro de Investigação em Agronomia, Alimentos, Ambiente e Paisagem, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
authors.forename: Maria Elisabete F
authors.initials: ME
authors.lastname: Silva
authors.affiliation: LEPABE, Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Lopes
authors.affiliation: LEAF, Centro de Investigação em Agronomia, Alimentos, Ambiente e Paisagem, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Cunha-Queda
authors.affiliation: LEPABE, Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal. Electronic address: opnunes@fe.up.pt.
authors.forename: Olga C
authors.initials: OC
authors.lastname: Nunes
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D058645:Denaturing Gradient Gel Electrophoresis; D064307:Microbiota; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D062611:Solid Waste / Q000032:analysis
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D062611:Solid Waste
keywords: 454-pyrosequencing; Bacterial community; DGGE profiling; Diversity; Multivariate analysis