title: Transgenic poplar overexpressing the endogenous transcription factor ERF76 gene improves salinity tolerance.
abstract: The ethylene response factor (ERF) family is one of the largest plant-specific transcription factor families, playing an important role in plant development and response to stresses. The ERF76 gene is a member of the poplar ERF transcription factor gene family. First, we validated that the ERF76 gene expressed in leaf and root tissues is responsive to salinity stress. We then successfully cloned the ERF76 cDNA fragment containing an open reading frame from di-haploid Populus simonii × Populus nigra and proved that ERF76 protein is targeted to the nucleus. Finally, we transferred the gene into the same poplar clone by the Agrobacterium-mediated leaf disc method. Using both RNA-Seq and reverse transcription-quantitative polymerase chain reaction, we validated that expression level of ERF76 is significantly higher in transgenic plants than that in the nontransgenic control. Using RNA-Seq data, we have identified 375 genes that are differentially expressed between the transgenic plants and the control under salt treatment. Among the differentially expressed genes, 16 are transcription factor genes and 45 are stress-related genes, both of which are upregulated significantly in transgenic plants, compared with the control. Under salt stress, the transgenic plants showed significant increases in plant height, root length, fresh weight, and abscisic acid (ABA) and gibberellin (GA) concentration compared with the control, suggesting that overexpression of ERF76 in transgenic poplar upregulated the expression of stress-related genes and increased the ability of ABA and GA biosynthesis, which resulted in stronger tolerance to salt stress.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China.
authors.forename: Shengji
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China.
authors.forename: Boru
authors.initials: B
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China tbjiang@yahoo.com.
authors.forename: Tingbo
authors.initials: T
authors.lastname: Jiang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D060054:Agrobacterium; D018533:Biomass; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development; D030821:Plants, Genetically Modified; D032107:Populus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics*; D014170:Transformation, Genetic; D014197:Trees; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D005875:Gibberellins; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid
keywords: ERF transcription factor; Populus simonii × P. nigra; RNA-Seq; genetic transformation; salt tolerance