title: A Rapid and Automated Device for Purifying Nucleic Acids.
abstract: We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.
authors.affiliation: Core Technology Development Center, Corporate Research and Development Div., Seiko Epson Corporation.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Uehara
authors.forename: Masato
authors.initials: M
authors.lastname: Hanamura
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yamada
authors.forename: Akemi
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: Toshiro
authors.initials: T
authors.lastname: Murayama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Saito
authors.forename: Kotaro
authors.initials: K
authors.lastname: Idegami
authors.forename: Takayuki
authors.initials: T
authors.lastname: Honda
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D009980:Influenza A virus / Q000235:genetics; D007251:Influenza, Human / Q000821:virology; D008280:Magnetics; D009696:Nucleic Acids / Q000302:isolation & purification*; D010614:Pharynx / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D009696:Nucleic Acids; D012367:RNA, Viral