title: Sorodiplophrys stercorea: Another Novel Lineage of Sorocarpic Multicellularity.
abstract: Sorodiplophrys stercorea is a sorocarpic organism that utilizes filose pseudopodia for locomotion and absorptive nutrition. It has traditionally been considered to be a member of the Labyrinthulae based on its morphology. Its closest relatives were thought to be species in the taxon Diplophrys. Since the genus Diplophrys has been shown to be paraphyletic and S. stercorea has pseudopodia similar to some members of Rhizaria, we examined its relationship with other eukaryotes. We obtained four isolates from the dung of cow and horse, brought each into monoeukaryotic culture, and sequenced their SSU rRNA gene for phylogenetic analysis. All our isolates were shown to form a monophyletic group in the Labyrinthulae, nested in the Amphifiloidea clade. Our results demonstrate that Sorodiplophrys is more closely related to species of the genus Amphifila than to Diplophrys and represents an additional independent origin of sorocarpic multicellularity among eukaryotes. This study represents the first confirmed sorocarpic lifestyle in the Stramenopiles.
authors.affiliation: Department of Biological Sciences, Mississippi State University, Mississippi State, 39762, Mississippi.|Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, 39762, Mississippi.|Department of Biological Sciences, University of Arkansas, Fayetteville, 72701, Arkansas.
authors.forename: Alexander K
authors.initials: AK
authors.lastname: Tice
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, 72701, Arkansas.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Silberman
authors.affiliation: Department of Biological Sciences, Mississippi State University, Mississippi State, 39762, Mississippi.
authors.forename: Austin C
authors.initials: AC
authors.lastname: Walthall
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, 72701, Arkansas.
authors.forename: Khoa N D
authors.initials: KN
authors.lastname: Le
authors.affiliation: Department of Biological Sciences, University of Arkansas, Fayetteville, 72701, Arkansas.
authors.forename: Frederick W
authors.initials: FW
authors.lastname: Spiegel
authors.affiliation: Department of Biological Sciences, Mississippi State University, Mississippi State, 39762, Mississippi.|Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, 39762, Mississippi.
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Brown
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000469:parasitology; D016054:DNA, Protozoan / Q000302:isolation & purification; D004275:DNA, Ribosomal; D020459:Genes, rRNA / Q000235:genetics; D006736:Horses / Q000469:parasitology; D008922:Mississippi; D010802:Phylogeny; D056901:Rhizaria / Q000145:classification; D058009:Stramenopiles / Q000145:classification* / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal
keywords: Aggregation; Labyrinthulomycetes; SSU rDNA; Stramenopiles; amoeba