title: Molecular basis for the thermostability of Newcastle disease virus.
abstract: Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. However, the genetic basis underlying the NDV thermostability is poorly understood. In this study, we generated chimeric viruses by exchanging viral genes between the thermostable TS09-C strain and thermolabile LaSota strain using reverse genetics technology. Evaluations of these chimeric NDVs demonstrated that the thermostability of NDV was dependent on the origin of HN protein. Chimeras bearing the HN protein derived from thermostable virus exhibited a thermostable phenotype, and vice versa. Both hemagglutinin and neuraminidase activities of viruses bearing the TS09-C HN protein were more thermostable than those containing LaSota HN protein. Furthermore, the newly developed thermostable virus rLS-T-HN, encoding the TS09-C HN protein in LaSota backbone, induced significantly higher antibody response than the TS09-C virus, and conferred complete protection against virulent NDV challenge. Taken together, the data suggest that the HN protein of NDV is a crucial determinant of thermostability, and the HN gene from a thermostable NDV could be engineered into a thermolabile NDV vaccine strain for developing novel thermostable NDV vaccine.
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.|US National Poultry Research Center, Agricultural Research Services, United States Department of Agriculture, Southeast Poultry Research Laboratory, Athens, GA 30605, USA.|Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Wuhan 430070, China.
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Wen
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.|State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.|State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Wang
authors.affiliation: US National Poultry Research Center, Agricultural Research Services, United States Department of Agriculture, Southeast Poultry Research Laboratory, Athens, GA 30605, USA.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Zhang
authors.affiliation: US National Poultry Research Center, Agricultural Research Services, United States Department of Agriculture, Southeast Poultry Research Laboratory, Athens, GA 30605, USA.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Zishu
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
authors.forename: Huabin
authors.initials: H
authors.lastname: Shao
authors.affiliation: US National Poultry Research Center, Agricultural Research Services, United States Department of Agriculture, Southeast Poultry Research Laboratory, Athens, GA 30605, USA.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000818:Animals; D002642:Chick Embryo; D006224:Cricetinae; D015707:HN Protein / Q000737:chemistry / Q000235:genetics; D006358:Hot Temperature; D009522:Newcastle disease virus / Q000737:chemistry / Q000235:genetics; D014765:Viral Vaccines / Q000737:chemistry / Q000235:genetics
chemical_list: D015707:HN Protein; D014765:Viral Vaccines