title: Biogas production and microbial community shift through neutral pH control during the anaerobic digestion of pig manure.
abstract: Laboratory-scale reactors, in which the pH could be auto-adjusted, were employed to investigate the mesophilic methane fermentation with pig manure (7.8% total solids) at pH 6.0, 7.0, and 8.0. Results showed that the performance of anaerobic digestion was strongly dependent on pH value. Biogas production and methane content at neutral pH 7.0 were significantly higher (16,607mL, 51.81%) than those at pH 6.0 (6916mL, 42.9%) and 8.0 (9739mL, 35.6%). Denaturing gradient gel electrophoresis fingerprinting and Shannon's index indicated that the samples contained highly diverse microbial communities. The major genus at pH 7.0 was Methanocorpusculum, compared with that was Methanosarcina at both pH 6.0 and 8.0. Our research revealed that cultures maintained at pH 7.0 could support increased biogas production, which has significant implications for the scale-up biogas engineering.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Environmental Engineering Laboratory, College of Resource and Environment, Shanxi Agricultural University, Taigu 030801, China.
authors.forename: Fenwu
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Yong
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Xiayuan
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zheng
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China. Electronic address: hhjia@njtech.edu.cn.
authors.forename: Honghua
authors.initials: H
authors.lastname: Jia
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D056804:Biofuels; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008372:Manure; D008697:Methane / Q000096:biosynthesis*; D017018:Methanomicrobiales / Q000235:genetics / Q000378:metabolism; D017020:Methanosarcina / Q000235:genetics / Q000378:metabolism; D059013:Microbial Consortia / Q000502:physiology*; D034421:Sus scrofa; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D056804:Biofuels; D008372:Manure; D008697:Methane
keywords: Anaerobic digestion; Biogas; DGGE; Microbial community; pH