title: Neonatal exposure to benzo[a]pyrene induces oxidative stress causing altered hippocampal cytomorphometry and behavior during early adolescence period of male Wistar rats.
abstract: Environmental neurotoxicants like benzo[a]pyrene (B[a]P) have been well documented regarding their potential to induce oxidative stress. However, neonatal exposure to B[a]P and its subsequent effect on anti-oxidant defence system and hippocampal cytomorphometry leading to behavioral changes have not been fully elucidated. We investigated the effect of acute exposure of B[a]P on five days old male Wistar pups administered with single dose of B[a]P (0.2 μg/kg BW) through intracisternal mode. Control group was administered with vehicle i.e., DMSO and a separate group of rats without any treatment was taken as naive group. Behavioral analysis showed anxiolytic-like behavior with significant increase in time spent in open arm in elevated plus maze. Further, significant reduction in fall off time during rotarod test showing B[a]P induced locomotor hyperactivity and impaired motor co-ordination in adolescent rats. B[a]P induced behavioral changes were further associated with altered anti-oxidant defence system involving significant reduction in the total ATPase, Na(+) K(+) ATPase, Mg(2+) ATPase, GR and GPx activity with a significant elevation in the activity of catalase and GST as compared to naive and control groups. Cytomorphometry of hippocampus showed that the number of neurons and glia in B[a]P treated group were significantly reduced as compared to naive and control. Subsequent observation showed that the area and perimeter of hippocampus, hippocampal neurons and neuronal nucleus were significantly reduced in B[a]P treated group as compared to naive and control. The findings of the present study suggest that the alteration in hippocampal cytomorphometry and neuronal population associated with impaired antioxidant signaling and mood in B[a]P treated group could be an outcome of neuromorphological alteration leading to pyknotic cell death or impaired differential migration of neurons during early postnatal brain development.
authors.affiliation: Department of Zoology, School of Life Sciences, Ravenshaw University, Odisha, India.
authors.forename: Bhupesh
authors.initials: B
authors.lastname: Patel
authors.affiliation: Department of Zoology, School of Life Sciences, Ravenshaw University, Odisha, India; Defence Institute of High Altitude Research, DRDO, Jammu and Kashmir, India.
authors.forename: Saroj Kumar
authors.initials: SK
authors.lastname: Das
authors.affiliation: Department of Zoology, School of Life Sciences, Ravenshaw University, Odisha, India.
authors.forename: Swagatika
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Zoology, School of Life Sciences, Ravenshaw University, Odisha, India.
authors.forename: Lipsa
authors.initials: L
authors.lastname: Das
authors.affiliation: Department of Zoology, School of Life Sciences, Ravenshaw University, Odisha, India. Electronic address: mpatri@ravenshawuniversity.ac.in.
authors.forename: Manorama
authors.initials: M
authors.lastname: Patri
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002452:Cell Count; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008124:Locomotion / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D009457:Neuroglia / Q000473:pathology; D009474:Neurons / Q000473:pathology; D009498:Neurotoxins / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D011596:Psychomotor Disorders / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D009498:Neurotoxins; D001564:Benzo(a)pyrene; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D000251:Adenosine Triphosphatases
keywords: Anxiety; Benzo[a]pyrene; Cytomorphometry; Hippocampus; Motor co-ordination; Oxidative stress