title: Protections of bovine serum albumin protein from damage on functionalized graphene-based electrodes by flavonoids.
abstract: A sensitive electrochemical sensor based on bovine serum albumin (BSA)/poly (diallyldimethylammonium chloride) (PDDA) functionalized graphene nanosheets (PDDA-G) composite film modified glassy carbon electrode (BSA/PDDA-G/GCE) had been developed to investigate the oxidative protein damage and protections of protein from damage by flavonoids. The performance of this sensor was remarkably improved due to excellent electrical conductivity, strong adsorptive ability, and large effective surface area of PDDA-G. The BSA/PDDA-G/GCE displayed the greatest degree of BSA oxidation damage at 40 min incubation time and in the pH 5.0 Fenton reagent system (12.5 mM FeSO4, 50 mM H2O2). The antioxidant activities of four flavonoids had been compared by fabricated sensor based on the relative peak current ratio of SWV, because flavonoids prevented BSA damage caused by Fenton reagent and affected the BSA signal in a solution containing Co(bpy)3(3+). The sensor was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). UV-vis spectrophotometry and FTIR were also used to investigate the generation of hydroxyl radical and BSA damage, respectively. On the basis of results from electrochemical methods, the order of the antioxidant activities of flavonoids is as follows: (+)-catechin>kaempferol>apigenin>naringenin. A novel, direct SWV analytical method for detection of BSA damage and assessment of the antioxidant activities of four flavonoids was developed and this electrochemical method provided a simple, inexpensive and rapid detection of BSA damage and evaluation of the antioxidant activities of samples.
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Bolu
authors.initials: B
authors.lastname: Sun
authors.affiliation: Lanzhou Military Command Center for Disease Prevention and Control, Lanzhou 730000, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Gou
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Xue
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zheng
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China. Electronic address: hufd@lzu.edu.cn.
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 51515, China. Electronic address: wanghongzhao@sina.com.
authors.forename: Wanghong
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D002417:Cattle; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D005419:Flavonoids / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry; D010084:Oxidation-Reduction; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; C045076:Fenton's reagent; D005419:Flavonoids; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012710:Serum Albumin, Bovine; D006108:Graphite; D006861:Hydrogen Peroxide; D007501:Iron
keywords: Antioxidant activities; Bovine serum albumin; Electrochemical sensor; Flavonoids; Square wave voltammetry (SWV)