title: Prophylaxis With a Middle East Respiratory Syndrome Coronavirus (MERS-CoV)-Specific Human Monoclonal Antibody Protects Rabbits From MERS-CoV Infection.
abstract: With >1600 documented human infections with Middle East respiratory syndrome coronavirus (MERS-CoV) and a case fatality rate of approximately 36%, medical countermeasures are needed to prevent and limit the disease. We examined the in vivo efficacy of the human monoclonal antibody m336, which has high neutralizing activity against MERS-CoV in vitro. m336 was administered to rabbits intravenously or intranasally before infection with MERS-CoV. Prophylaxis with m336 resulted in a reduction of pulmonary viral RNA titers by 40-9000-fold, compared with an irrelevant control antibody with little to no inflammation or viral antigen detected. This protection in rabbits supports further clinical development of m336.
authors.affiliation: Laboratory of Infectious Diseases.
authors.forename: Katherine V
authors.initials: KV
authors.lastname: Houser
authors.affiliation: Laboratory of Infectious Diseases.
authors.forename: Lisa
authors.initials: L
authors.lastname: Gretebeck
authors.affiliation: Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College, Fudan University, China.
authors.forename: Tianlei
authors.initials: T
authors.lastname: Ying
authors.affiliation: Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, NIH, Frederick, Maryland.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Infectious Diseases.
authors.forename: Leatrice
authors.initials: L
authors.lastname: Vogel
authors.affiliation: Laboratory of Infectious Diseases.
authors.forename: Elaine W
authors.initials: EW
authors.lastname: Lamirande
authors.affiliation: Comparative Medicine Branch, Infectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Disease, National Institutes of Health (NIH), Bethesda.
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Bock
authors.affiliation: Comparative Medicine Branch, Infectious Disease Pathogenesis Section, National Institute of Allergy and Infectious Disease, National Institutes of Health (NIH), Bethesda.
authors.forename: Ian N
authors.initials: IN
authors.lastname: Moore
authors.affiliation: Protein Interactions Section, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, NIH, Frederick, Maryland.
authors.forename: Dimiter S
authors.initials: DS
authors.lastname: Dimitrov
authors.affiliation: Laboratory of Infectious Diseases.
authors.forename: Kanta
authors.initials: K
authors.lastname: Subbarao
mesh_terms: D000281:Administration, Intranasal; D061605:Administration, Intravenous; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000627:therapeutic use*; D057134:Antibodies, Neutralizing / Q000097:blood / Q000627:therapeutic use; D000914:Antibodies, Viral / Q000097:blood / Q000627:therapeutic use*; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D016756:Immunoglobulins, Intravenous; D008168:Lung / Q000821:virology; D065207:Middle East Respiratory Syndrome Coronavirus / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000032:analysis; D011817:Rabbits
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D016756:Immunoglobulins, Intravenous; D012367:RNA, Viral
keywords: MERS-CoV; human mAb; m336; prophylaxis; rabbits