title: Estradiol and angiotensin II crosstalk in hydromineral balance: Role of the ERK1/2 and JNK signaling pathways.
abstract: The angiotensin II (ANGII) receptor AT1 plays an important role in the control of hydromineral balance, mediating the dipsogenic and natriorexigenic effects and neuroendocrine responses of ANGII. While estradiol (E2) is known to modulate several actions of ANGII in the brain, the molecular and cellular mechanisms of the interaction between E2 and ANGII and its physiological role in the control of body fluids remain unclear. We investigated the influence of E2 (40 μg/kg) pretreatment and extracellular-signal-regulated kinase (ERK1/2) and c-Jun N-terminal kinase (JNK) cell signaling on the dipsogenic and natriorexigenic effects, as well as the neuroendocrine responses to angiotensinergic central stimulation in ovariectomized rats (OVX). We showed that the inhibitory effect of E2 on ANGII-induced water and sodium intake requires the ERK1/2 and JNK signaling pathways. On the other hand, E2 pretreatment prevents the ANGII-induced phosphorylation of ERK and JNK in the lamina terminalis. E2 therapy decreased oxytocin (OT) and vasopressin (AVP) secretion and decreased ERK1/2 phosphorylation in the supraoptic and paraventricular nuclei (SON and PVN, respectively). We found that the AVP secretion induced by ANGII required ERK1/2 signaling, but OT secretion did not involve ERK1/2 signaling. Taken together, these results demonstrate that E2 modulates ANGII-induced water and sodium intake and AVP secretion by affecting the ERK1/2 and JNK pathways in the lamina terminalis and ERK1/2 signaling in the hypothalamic nuclei (PVN and SON) in OVX rats.
authors.affiliation: Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 14049-900, Brazil. Electronic address: g.almeidapereira@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Almeida-Pereira
authors.affiliation: Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 14049-900, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Coletti
authors.affiliation: Department of Physiological Sciences, Institute of Biology, Federal Rural University of Rio de Janeiro, Seropedica 23890-000, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Mecawi
authors.affiliation: Department of Physiological Sciences, Institute of Biology, Federal Rural University of Rio de Janeiro, Seropedica 23890-000, Brazil.
authors.forename: L C
authors.initials: LC
authors.lastname: Reis
authors.affiliation: Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 14049-900, Brazil.
authors.forename: L L K
authors.initials: LL
authors.lastname: Elias
authors.affiliation: Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 14049-900, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Antunes-Rodrigues
mesh_terms: D000804:Angiotensin II / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D002491:Central Nervous System Agents / Q000008:administration & dosage / Q000378:metabolism; D004326:Drinking / Q000187:drug effects / Q000502:physiology*; D004958:Estradiol / Q000008:administration & dosage / Q000378:metabolism*; D005260:Female; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology*; D010052:Ovariectomy; D010121:Oxytocin / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism; D011897:Random Allocation; D017208:Rats, Wistar; D012982:Sodium, Dietary; D013495:Supraoptic Nucleus / Q000187:drug effects / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism
chemical_list: D002491:Central Nervous System Agents; D012982:Sodium, Dietary; D014667:Vasopressins; D000804:Angiotensin II; D004958:Estradiol; D010121:Oxytocin; D048031:JNK Mitogen-Activated Protein Kinases
keywords: ovariectomized rats; oxytocin secretion; sodium intake; vasopressin secretion; water intake