title: The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells.
abstract: Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic differentiation potential of mesenchymal stem cells. The aim of this study was to assess the age-related maintenance of physiological function and differentiation potential of hASCs in vitro. hASCs were isolated from patients of four different age groups: (1) >20 years (n = 7), (2) >50 years (n = 7), (3) >60 years (n = 7), and (4) >70 years (n = 7). The hASCs were characterized according to the number of fibroblasts colony forming unit (CFU-F), proliferation rate, population doubling time (PDT), and quantified parameters of adipogenic, chondrogenic, and osteogenic differentiation. Compared to younger cells, aged hASCs had decreased proliferation rates, decreased chondrogenic and osteogenic potential, and increased senescent features. A shift in favor of adipogenic differentiation with increased age was also observed. As many bone and joint diseases increase in prevalence with age, it is important to consider the negative influence of age on hASCs viability, proliferation status, and multilineage differentiation potential when considering the potential therapeutic applications of hASCs.
authors.affiliation: Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland; Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland.
authors.forename: Monika
authors.identifier: 0000-0002-7281-9272
authors.initials: M
authors.lastname: Marędziak
authors.affiliation: Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland; Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
authors.affiliation: Department of Anatomy, Jagiellonian University Medical College, 31-034 Krakow, Poland.
authors.forename: Krzysztof A
authors.identifier: 0000-0001-5077-6948
authors.initials: KA
authors.lastname: Tomaszewski
authors.affiliation: Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland.
authors.forename: Katarzyna
authors.identifier: 0000-0002-2311-5789
authors.initials: K
authors.lastname: Kornicka
authors.affiliation: Department of Anatomy, Jagiellonian University Medical College, 31-034 Krakow, Poland.
authors.forename: Brandon Michael
authors.initials: BM
authors.lastname: Henry