title: Biomimetic scaffolds containing nanofibers coated with willemite nanoparticles for improvement of stem cell osteogenesis.
abstract: Nowadays, discovering osteogenesis stimulating effectors is one of the major topics in bone tissue engineering and regenerative medicine. In this study, the proliferation rate and osteogenic differentiation potency of adipose-derived mesenchymal stem cells (AT-MSCs) cultured on poly (l-lactide acid) (PLLA) and willemite-coated PLLA were investigated by MTT assay and common osteogenic markers such as alkaline phosphatase (ALP) activity, calcium mineral deposition and bone-related genes expression. Willemite-coated PLLA showed a higher proliferation support to AT-MSCs in comparison to PLLA and TCPS. During the period of study, AT-MSCs cultured on willemite-coated PLLA scaffolds exhibited the greatest ALP activity and mineralization. Gene expression analysis demonstrated that the highest expression of four important osteogenic-related genes, osteonectin, Runx2, collagen type 1 and osteocalcin was observed in stem cells cultured on willemite-coated PLLA nanofibrous scaffolds. According to the results, willemite-coated PLLA could be a suitable substrate to support the proliferation and osteogenic differentiation of stem cells and holds promising potential for bone tissue engineering and regenerative medicine applications.
authors.affiliation: Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Rouhallah
authors.initials: R
authors.lastname: Ramezanifard
authors.affiliation: Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran. Electronic address: seyedjafari@ut.ac.ir.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.affiliation: Department of Stem Cell Biology, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.affiliation: Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000293:Adolescent; D000328:Adult; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D017640:Silicates; D017967:Zinc Compounds; C029500:zinc silicate
keywords: Bioceramic; Electrospinning; Nanoparticle; Osteogenesis; Tissue engineering; Willemite