title: Induction of Asherman's Syndrome in Rabbit.
abstract: BACKGROUND
  Uterine synechiae or Asherman's syndrome is a condition that can cause infertility. The present experimental study was designed to establish the rabbit as an animal model for human Asherman's syndrome using the endometrial curettage.
  
  
  METHODS
  In an experimental study, female adult rabbits (n=18) were randomly divided into intact and ovariectomized groups. One third of caudal part of both uteri was submitted to traumatic endometrial curettage. One group was simultaneously ovariectomized. The intact rabbits were artificially induced ovulation during 10 days after surgery. One third of cranial part of both uteri was selected as the control. Synechiae occurring, luminal area/total area (LA/TA), endometrial area/total area (EA/TA), myometrial and perimetrial area/total area (MPA/TA), endometrial area/uterine wall area (EA/UWA), and myometrial and perimetrial area/uterine wall area (MPA/UWA) ratios of both uteri in six subdivided groups (n=6) were analysed in curetted and intact control parts. On days 15, 30 and 45 following surgery by two-way ANOVA and LSD test (p<0.05).
  
  
  RESULTS
  Histopathologic findings showed significant epithelial damage together with significant inflammatory reaction in the intact curettage group. The LA/TA ratios of the intact curettage group on days 15 and 45 were more than the intact control group on day 15. The EA/TA ratio of the intact curettage group on day 30 was less than the intact control group on day 30.
  
  
  CONCLUSIONS
  Uterine fibrosis was observed in intact curettage group, and this modified animal model showed a pathogenesis condition similar to intrauterine adhesions observed in human.
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Bazoobandi
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Pharmacology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Nader
authors.initials: N
authors.lastname: Tanideh
authors.affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Rahmanifar
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Tamadon
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Keshtkar
authors.affiliation: Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: Perinatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Obstetrics & Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Kasraeian
authors.affiliation: Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Koohi-Hosseinabadi
keywords: Animal model; Asherman's syndrome; Rabbit; Uterine synechia