title: Methanolic Extract of Pien Tze Huang Induces Apoptosis Signaling in Human Osteosarcoma MG63 Cells via Multiple Pathways.
abstract: Pien Tze Huang (PZH) is a well-known traditional Chinese formulation and has long been used as an alternative remedy for cancers in China and Southeast Asia. Recently, antitumor activity of PZH on several tumors have been increasingly reported, but its antitumor activity and the possible action mechanism on osteosarcoma remains unclear. After treatment with PZH, cell viability of MG-63 cells was dose-dependently inhibited compared to control cells. Moreover, a DNA ladder characteristic of apoptosis was observed in the cells treated with PZH, especially 500 μg/mL, 750 μg/mL. Further investigation showed that PZH treatments led to activation of caspase cascades and changes of apoptotic mediators Bcl2, Bax, and Bcl-xL expression. In addition, our results suggested that PZH activated PI3K/Akt signal pathway, and the phosphorylation of Akt and ERK1/2 were associated with the induction of apoptotic signaling. These results revealed that PZH possesses antitumoral activity on human osteosarcoma MG63 cells by manipulating apoptotic signaling and multiple pathways. It is suggested that PZH alone or combined with regular antitumor drugs may be beneficial as osteosarcoma treatments.
authors.affiliation: College of Osteopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. fyfjtcm@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fu
authors.affiliation: College of Osteopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. zlfjtcm@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Osteopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. zzpzhhf@163.com.
authors.forename: Zhenqiang
authors.initials: Z
authors.lastname: Hong
authors.affiliation: Integrative Medicine College, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou 350122, China. zhyfjtcm@gmail.com.
authors.forename: Haiyin
authors.initials: H
authors.lastname: Zheng
authors.affiliation: College of Osteopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. hmfjtcm@sina.com.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Osteopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. zzpzhhjm@163.com.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Gao
authors.affiliation: College of Osteopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. cbyfjtcm@sina.com.
authors.forename: Boyi
authors.initials: B
authors.lastname: Chen
authors.affiliation: College of Osteopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China. zyfjtcm@sina.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D001859:Bone Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans; D000432:Methanol / Q000494:pharmacology*; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D019253:Proto-Oncogene Proteins c-bcl-2; C539975:pien tze huang; D020169:Caspases; D000432:Methanol
keywords: Bax; Bcl-2; PI3K; Pien Tze Huang; caspase-3; caspase-9; osteosarcoma; p-Akt