title: Development and characterization of microsatellite markers for the oomyceta Aphanomyces euteiches.
abstract: Aphanomyces euteiches Drechsler is a serious pathogen of leguminous crops that causes devastating root rot of pea worldwide. Given that A. euteiches is a diploid organism, robust, codominant markers are needed for population genetics studies. We have developed and screened a microsatellite-enriched small-insert genomic library for identification of A. euteiches SSR containing sequences. Fourteen out of the 48 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of 94 isolates. The number of alleles at each locus ranged from one to four. The identification of new markers would enhance the ability to evaluate the genetic structure of A. euteiches populations, and pathogen evolution.
authors.affiliation: INRA, UMR1349 Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Domaine de la Motte, 35653 Le Rheu, France; Agrocampus Ouest, Laboratoire Ecologie et Santé des Plantes (ESP), 65 rue de Saint Brieuc, 35042 Rennes, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Mieuzet
authors.affiliation: INRA, UMR1349 Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Domaine de la Motte, 35653 Le Rheu, France; Agrocampus Ouest, Laboratoire Ecologie et Santé des Plantes (ESP), 65 rue de Saint Brieuc, 35042 Rennes, France.
authors.forename: A
authors.initials: A
authors.lastname: Quillévéré
authors.affiliation: INRA, UMR1349 Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Domaine de la Motte, 35653 Le Rheu, France; Agrocampus Ouest, Laboratoire Ecologie et Santé des Plantes (ESP), 65 rue de Saint Brieuc, 35042 Rennes, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Pilet
authors.affiliation: INRA, UMR1349 Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Domaine de la Motte, 35653 Le Rheu, France; Agrocampus Ouest, Laboratoire Ecologie et Santé des Plantes (ESP), 65 rue de Saint Brieuc, 35042 Rennes, France; Université de Rennes I, 9 rue Jean Macé, 35700 Rennes, France. Electronic address: lemay@agrocampus-ouest.fr.
authors.forename: C
authors.initials: C
authors.lastname: Le May
mesh_terms: D000483:Alleles; D044744:Aphanomyces / Q000235:genetics* / Q000472:pathogenicity; D002874:Chromosome Mapping; D018895:Microsatellite Repeats / Q000235:genetics*; D018532:Pisum sativum / Q000235:genetics / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology
keywords: Aphanomyces; Genetic study; Microsatellite; Oomyceta; SSR