title: Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population.
abstract: BACKGROUND
  Type 2 diabetes mellitus (T2DM) is an inflammatory autoimmune disease that mostly affects older adults. The etiology of T2DM includes both genetic and environmental factors. rs1800795 (-174 G/C) single nucleotide polymorphism (SNP) linked with autoimmune disorders predispositions, identified by Genome-Wide Association Study among genes, which immunologically related is considerably over signified. The goal of this study was to evaluate the association between rs1800795 (-174 G/C) polymorphisms in the promoter of interleukin-6 (IL-6) gene with susceptibility to T2DM in a subset of the Iranian population.
  
  
  METHODS
  In this case-control study, 120 healthy subjects and 120 patients with T2DM were included. Genomic DNA obtained from whole blood samples and the polymerase chain reaction was used to amplify the fragment of interest contain rs1800795 SNP, restriction fragment length polymorphism method was applied for genotyping of the DNA samples with NlaIII as a restriction enzyme. SPSS for Windows software (version 18.0, SPSS, Chicago, IL, USA) was performed for statistical analysis.
  
  
  RESULTS
  No significant differences were found between healthy controls and T2DM patients with respect to the frequency distribution of the cytokine gene polymorphism investigated. Odds ratio, adjusted for sex, age, and smoking status has displayed similar outcomes.
  
  
  CONCLUSIONS
  These results indicated that the rs1800795 SNP is not a susceptibility gene variant for the development of T2DM in the Isfahan population. Further studies using new data on complex transcriptional interactions between IL-6 polymorphic sites are necessary to determine IL-6 haplotype influence on susceptibility to T2DM.
authors.affiliation: Department of Pharmaceutical Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghavimi
authors.affiliation: Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Sharifi
authors.affiliation: Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Mohaghegh
authors.affiliation: Department of Pharmaceutical Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mohammadian
authors.affiliation: Department of Biology, Kurdistan Science and Research Branch, Islamic Azad University, Sanandaj, Iran.
authors.forename: Saedeh
authors.initials: S
authors.lastname: Khadempar
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Hamzeh
authors.initials: H
authors.lastname: Rezaei
keywords: Genome-wide association study; interleukin-6 gene; polymorphism; type 2 diabetes mellitus