title: Effect of Complexin II on Membrane Fusion between Liposomes Containing Mast Cell SNARE Proteins.
abstract: Mast cells are involved in allergic responses and undergo exocytotic release of inflammatory mediators in response to antigen stimulation. Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins are involved in this membrane fusion process; some SNARE-binding proteins regulate SNARE-dependent liposome membrane fusion. SNARE-binding protein complexin II is expressed in mast cells, where it positively regulates exocytotic release after antigen stimulation. We found that complexin II suppressed SNARE-dependent membrane fusion between mast cell SNARE-containing liposomes. This inhibitory effect of complexin II was abolished when we used a structurally divergent mutant (R59H) complexin II, where Arg59 is substituted with histidine. These results suggest that complexin II negatively regulates SNARE-dependent exocytotic membrane fusion in mast cells, and this inhibitory effect is dependent upon Arg59.
authors.affiliation: Faculty of Pharma Sciences, Teikyo University.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tadokoro
authors.forename: Naohide
authors.initials: N
authors.lastname: Hirashima
authors.forename: Naoko
authors.initials: N
authors.lastname: Utsunomiya-Tate
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D001120:Arginine / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008081:Liposomes / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism; D008561:Membrane Fusion / Q000187:drug effects; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D050600:SNARE Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D008081:Liposomes; D009419:Nerve Tissue Proteins; D050600:SNARE Proteins; C095993:complexin II; D001120:Arginine