title: Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes.
abstract: Tendon adhesion is a common problem in the healing of injured tendons. The molecular mechanisms of the TGF-β/Smad signaling pathway have been determined, and the role of TGF-β has been well characterized in wound healing. However, the intracellular mechanism or downstream signals by which TGF-β3 modulates its effects on tendon healing have not been well elucidated. The aim of this study was to determine the effect of TGF‑β3 on the TGF-β/Smad signaling pathway in tenocytes. Quantitative polymerase chain reaction and western blot analysis were used to analyze the effect of TGF‑β3 on the regulation of the expression of Smad proteins in tenocytes. The results demonstrated that TGF‑β3 has no significant effect on the proliferation of tendon cells. The addition of TGF‑β3 to tenocytes can significantly downregulate the expression of Smad3 and upregulate the expression of Smad7 at the gene and protein levels. The results demonstrate that TGF‑β3 may regulate Smad3 and Smad7 proteins through the TGF-β/Smad signaling pathway to minimize extrinsic scarring. Thus, it may provide a novel approach to decrease tendon adhesion and promote tendon healing.
authors.affiliation: Department of Orthopedics, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
authors.forename: Ke
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Department of Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China.
authors.forename: Guo
authors.initials: G
authors.lastname: Chun
authors.affiliation: Department of Orthopedics, Daping Hospital, The Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Daping Hospital, The Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Quanyin
authors.initials: Q
authors.lastname: Du
authors.affiliation: Department of Orthopedics, Daping Hospital, The Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Daping Hospital, The Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism*; D051906:Smad7 Protein / Q000235:genetics / Q000378:metabolism*; D000070916:Tenocytes / Q000166:cytology / Q000378:metabolism; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology*
chemical_list: D051900:Smad3 Protein; D051906:Smad7 Protein; D053782:Transforming Growth Factor beta3; D003609:Dactinomycin; D003513:Cycloheximide