title: Molecular endocrine changes of Gh/Igf1 axis in gilthead sea bream (Sparus aurata L.) exposed to different environmental salinities during larvae to post-larvae stages.
abstract: The influence of acclimation of the euryhaline gilthead sea bream (Sparus aurata) larvae/post-larvae to brackish water on growth, energetic contents, and mRNA levels of selected hormones and growth-regulating hypothalamic neurohormones was assessed. Specimens from 49 days post-hatching were acclimated during 28 days to two different environmental salinities: 38 and 20 psu (as brackish water). Both groups were then transferred to 38 psu and acclimated for an additional week. Early juveniles were sampled after 28 days of acclimation to both salinities and one week after transfer to 38 psu. Pituitary adenylate cyclase-activating peptide (adcyap1; pacap), somatostatin-I (sst1), growth hormone (gh1), insulin-like growth factor-I (igf1), and prolactin (prl) mRNA expression were all studied by QPCR. Post-larvae acclimated to 20 psu showed better growth performance and body energetic content than post-larvae maintained at 38 psu. prl, adcyap1, and igf1 mRNA expression levels increased in 20-psu-acclimated post-larvae but decreased upon transfer to 38 psu. GH1 expression did not show significant changes under both experimental conditions. Our results suggested an enhanced general performance for post-larvae in brackish water, supported by the actions of adcyap1, igf1, and prl.
authors.affiliation: Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (ICMAN-CSIC), Campus de Excelencia Internacional del Mar (CEIMAR), 11519, Puerto Real, Cádiz, Spain. khaled.mohammed@icman.csic.es.|Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Campus de Excelencia Internacional del Mar (CEIMAR), 11519, Puerto Real, Cádiz, Spain. khaled.mohammed@icman.csic.es.|Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Sciences, Menoufia University, Menoufia, Egypt. khaled.mohammed@icman.csic.es.
authors.forename: Khaled
authors.initials: K
authors.lastname: Mohammed-Geba
authors.affiliation: Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (ICMAN-CSIC), Campus de Excelencia Internacional del Mar (CEIMAR), 11519, Puerto Real, Cádiz, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Yúfera
authors.affiliation: Instituto de Ciencias Marinas de Andalucía, Consejo Superior Investigaciones Científicas (ICMAN-CSIC), Campus de Excelencia Internacional del Mar (CEIMAR), 11519, Puerto Real, Cádiz, Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Martínez-Rodríguez
authors.affiliation: Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Campus de Excelencia Internacional del Mar (CEIMAR), 11519, Puerto Real, Cádiz, Spain.
authors.forename: Juan Miguel
authors.initials: JM
authors.lastname: Mancera
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D013006:Growth Hormone / Q000235:genetics; D007031:Hypothalamus / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D007814:Larva / Q000235:genetics / Q000254:growth & development; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000235:genetics; D011388:Prolactin / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D054712:Salinity; D021541:Sea Bream / Q000235:genetics / Q000254:growth & development*; D013004:Somatostatin / Q000235:genetics
chemical_list: D029941:Fish Proteins; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger; D013004:Somatostatin; D007334:Insulin-Like Growth Factor I; D011388:Prolactin; D013006:Growth Hormone
keywords: Brackish water; Post-larvae; Sparus aurata; adcyap1; gh1; igf1; prl; sst1