title: Enzymatic Modification of Soluble Cyanophycin Using the Type II Peptidyl Arginine Deiminase from Oryctolagus cuniculus.
abstract: An increased structural variety expands the number of putative applications for cyanophycin (multi-l-arginyl-poly-[l-aspartic acid], CGP). Therefore, structural modifications of CGP are of major interest; these are commonly obtained by modification and optimization of the bacterial producing strain or by chemical modification. In this study, an enzymatic modification of arginine side chains from lysine-rich CGP is demonstrated using the peptidyl arginine deiminase from Oryctolagus cuniculus, purified from Escherichia coli after heterologous expression. About 10% of the arginine side chains are converted to citrulline which corresponds to 4% of the polymer's total side chains. An inhibition of the reaction in the presence of small amounts of l-citrulline is observed, thereby explaining the low conversion rate. CGP dipeptides can be modified with about 7.5 mol% of the Asp-Arg dipeptides being converted to Asp-Cit. These results show that the enzymatic modification of CGP is feasible, opening up a whole new area of possible CGP modifications for further research.
authors.affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Münster, Corrensstraße 3, 48149, Münster, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Wiefel
authors.affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Münster, Corrensstraße 3, 48149, Münster, Germany.|Environmental Science Department, King Abdulaziz University, 21589, Jeddah, Saudi Arabia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Steinbüchel
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry; D001426:Bacterial Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006867:Hydrolases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D000076342:Protein-Arginine Deiminases; D011817:Rabbits
chemical_list: D001426:Bacterial Proteins; D004151:Dipeptides; C024250:cyanophycin; D001120:Arginine; D006867:Hydrolases; D000076342:Protein-Arginine Deiminases
keywords: cyanophycin; dipeptides; enzymatic modification; peptidyl arginine deiminase