title: Virus-like particles of porcine bocavirus generated by recombinant baculoviruses can be applied to sero-epidemic studies.
abstract: Porcine bocaviruses (PBoVs), new members of the Bocavirus genus, have been identified in swine worldwide. However, the antigenicity and epidemiology of PBoVs are still unclear. Here we used a recombinant baculovirus expression system to express the main capsid protein VP2 of Japan strain JY31b in insect Tn5 cells, and successfully produced the virus-like particles of PBoV (PBoV-LPs). The diameter and densities of the PBoV-LPs were estimated to be 30nm and 1.300g/cm(3), respectively, which were similar to the values for the native virion of PBoV. Antigenic analysis demonstrated that the PBoV-LPs were not cross-reactive with porcine circovirus 2, but were cross-reactive with human bocavirus 1, 2, 3 and 4. An ELISA for detection of anti-PBoV IgG antibodies was established using PBoV-LPs as antigen, which proved to be useful for monitoring PBoV infection in both swine and wild boars. The preliminary epidemiology research showed that 90.7% of pigs and 59.5% of wild boars were positive for the anti-PBoV-IgG, suggesting that both species were also widely infected with PBoV. The seven PBoV strains detected in wild boars separated into four subgroups, demonstrating the genetic diversity of PBoV.
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan 250012, China; Blood Group Reference and Research Laboratory, Blood Center of Shandong Province, East Shanshi Road 22, Jinan, Shandong, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine and Life Science, Nippon Veterinary and Life Science University, Kyonan-cho 1-7-1, Musashino, Tokyo 180-8602, Japan.
authors.forename: Natsuha
authors.initials: N
authors.lastname: Sano
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Kataoka
authors.affiliation: Division of Experimental Animals Research, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ami
authors.affiliation: Division of Experimental Animals Research, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Suzaki
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan.
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.affiliation: Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine and Life Science, Nippon Veterinary and Life Science University, Kyonan-cho 1-7-1, Musashino, Tokyo 180-8602, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan. Electronic address: litc@nih.go.jp.
authors.forename: Tian-Cheng
authors.initials: TC
authors.lastname: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics*; D052660:Bocavirus / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D003429:Cross Reactions; D004279:DNA, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016036:Seroepidemiologic Studies; D061987:Sf9 Cells; D018411:Spodoptera; D034421:Sus scrofa; D013552:Swine; D014771:Virion / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D011994:Recombinant Proteins
keywords: ELISA; PBoV-LPs; Porcine Bocavirus; Recombinant baculovirus; Virus-like particles