title: Laminin Receptor-Avid Nanotherapeutic EGCg-AuNPs as a Potential Alternative Therapeutic Approach to Prevent Restenosis.
abstract: In our efforts to develop new approaches to treat and prevent human vascular diseases, we report herein our results on the proliferation and migration of human smooth muscles cells (SMCs) and endothelial cells (ECs) using epigallocatechin-3-gallate conjugated gold nanoparticles (EGCg-AuNPs) as possible alternatives to drug coated stents. Detailed in vitro stability studies of EGCg-AuNPs in various biological fluids, affinity and selectivity towards SMCs and ECs have been investigated. The EGCg-AuNPs showed selective inhibitory efficacy toward the migration of SMCs. However, the endothelial cells remained unaffected under similar experimental conditions. The cellular internalization studies have indicated that EGCg-AuNPs internalize into the SMCs and ECs within short periods of time through laminin receptor mediated endocytosis mode. Favorable toxicity profiles and selective affinity toward SMCs and ECs suggest that EGCg-AuNPs may provide attractive alternatives to drug coated stents and therefore offer new therapeutic approaches in treating cardiovascular diseases.
authors.affiliation: Department of Radiology, University of Missouri, Columbia, MO 65211, USA. khoobchandanim@health.missouri.edu.|Institute of Green Nanotechnology, University of Missouri, Columbia, MO 65211, USA. khoobchandanim@health.missouri.edu.
authors.forename: Menka
authors.initials: M
authors.lastname: Khoobchandani
authors.affiliation: Department of Radiology, University of Missouri, Columbia, MO 65211, USA. KattiKK@health.missouri.edu.|Institute of Green Nanotechnology, University of Missouri, Columbia, MO 65211, USA. KattiKK@health.missouri.edu.
authors.forename: Kavita
authors.initials: K
authors.lastname: Katti
authors.affiliation: Department of Medicine, University of Missouri, Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65211, USA. amn6f@health.missouri.edu.
authors.forename: Adam
authors.initials: A
authors.lastname: Maxwell
authors.affiliation: Department of Medicine, University of Missouri, Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65211, USA. fayw@health.missouri.edu.|Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO 65211, USA. fayw@health.missouri.edu.
authors.forename: William P
authors.initials: WP
authors.lastname: Fay
authors.affiliation: Department of Radiology, University of Missouri, Columbia, MO 65211, USA. KattiK@health.missouri.edu.|Institute of Green Nanotechnology, University of Missouri, Columbia, MO 65211, USA. KattiK@health.missouri.edu.|Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO 65211, USA. KattiK@health.missouri.edu.|Department of Physics, University of Missouri, Columbia, MO 65211, USA. KattiK@health.missouri.edu.|Department of Biological Engineering, University of Missouri, Columbia, MO 65211, USA. KattiK@health.missouri.edu.|University of Missouri Research Reactor, University of Missouri, Columbia, MO 65211, USA. KattiK@health.missouri.edu.
authors.forename: Kattesh V
authors.initials: KV
authors.lastname: Katti
mesh_terms: D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage* / Q000493:pharmacokinetics / Q000494:pharmacology*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D023903:Coronary Restenosis / Q000517:prevention & control; D004337:Drug Carriers / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017474:Receptors, Laminin / Q000378:metabolism; D012269:Ribosomal Proteins
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004337:Drug Carriers; C510624:RPSA protein, human; D017474:Receptors, Laminin; D012269:Ribosomal Proteins; D006046:Gold; D002392:Catechin; C045651:epigallocatechin gallate
keywords: atherosclerosis; epigallocatechin-3-gallate; gold nanoparticles; green synthesis