title: Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions.
abstract: Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.
authors.affiliation: Lomonosov Moscow State University, Faculty of Physics, Chair of General Physics and Wave Processes, Leninskie Gory 1/62, Moscow 119991, RussiabUniversity of Oulu, Faculty of Information Technology and Electrical Engineering, Opto-Electronics and Measureme.
authors.forename: Kisung
authors.initials: K
authors.lastname: Lee
authors.affiliation: University of Oulu, Faculty of Information Technology and Electrical Engineering, Opto-Electronics and Measurement Techniques, Erkki Koiso-Kanttilankatu 3, Oulu 90570, Finland.
authors.forename: Matti
authors.initials: M
authors.lastname: Kinnunen
authors.affiliation: Lomonosov Moscow State University, Faculty of Physics, Chair of Quantum Electronics, Leninkie Gory 1/62, Moscow 119991, Russia.
authors.forename: Maria D
authors.initials: MD
authors.lastname: Khokhlova
authors.affiliation: Lomonosov Moscow State University, Faculty of Physics, Chair of Quantum Electronics, Leninkie Gory 1/62, Moscow 119991, Russia.
authors.forename: Evgeny V
authors.initials: EV
authors.lastname: Lyubin
authors.affiliation: Lomonosov Moscow State University, Faculty of Physics, Chair of General Physics and Wave Processes, Leninskie Gory 1/62, Moscow 119991, RussiadLomonosov Moscow State University, International Laser Center, Leninskie Gory 1/62, Moscow 119991, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Priezzhev
authors.affiliation: University of Oulu, Faculty of Information Technology and Electrical Engineering, Opto-Electronics and Measurement Techniques, Erkki Koiso-Kanttilankatu 3, Oulu 90570, Finland.
authors.forename: Igor
authors.initials: I
authors.lastname: Meglinski
authors.affiliation: Lomonosov Moscow State University, Faculty of Physics, Chair of Quantum Electronics, Leninkie Gory 1/62, Moscow 119991, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Fedyanin
mesh_terms: D000328:Adult; D000418:Albumins / Q000494:pharmacology; D004867:Equipment Design; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D005340:Fibrinogen / Q000494:pharmacology*; D006801:Humans; D008297:Male; D052898:Optical Tweezers; D010949:Plasma / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000418:Albumins; D005340:Fibrinogen