title: Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes.
abstract: OBJECTIVE
  This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract.
  
  
  METHODS
  Bovine tendons were used for preparation of the extract and were stored at -80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR.
  
  
  RESULTS
  The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression.
  
  
  CONCLUSIONS
  Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.
authors.affiliation: Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brazil.
authors.forename: Lívia Maria Mendonça
authors.initials: LM
authors.lastname: Augusto
authors.affiliation: Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brazil.
authors.forename: Diego Pinheiro
authors.initials: DP
authors.lastname: Aguiar
authors.affiliation: Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brazil.
authors.forename: Danielle Cabral
authors.initials: DC
authors.lastname: Bonfim
authors.affiliation: Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brazil.
authors.forename: Amanda
authors.initials: A
authors.lastname: Dos Santos Cavalcanti
authors.affiliation: Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brazil.
authors.forename: Priscila Ladeira
authors.initials: PL
authors.lastname: Casado
authors.affiliation: Instituto Nacional de Traumatologia e Ortopedia, Rio de Janeiro, RJ, Brazil.
authors.forename: Maria Eugênia Leite
authors.initials: ME
authors.lastname: Duarte
keywords: Mesenchymal stromal cells from bone marrow; Tendon; Tenocytes