title: Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells.
abstract: The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 152-703, Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Deok-Won
authors.initials: DW
authors.lastname: Lee
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.forename: Kyu-Sik
authors.initials: KS
authors.lastname: Shim
authors.forename: Daniel I
authors.initials: DI
authors.lastname: Jeon
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Rhee
authors.forename: Hak-Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D016503:Drug Delivery Systems; D017886:Durapatite / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D007781:Lactoferrin / Q000008:administration & dosage*; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D012333:RNA, Messenger; D015675:Osteocalcin; D053495:Osteopontin; D006493:Heparin; D017886:Durapatite; D000469:Alkaline Phosphatase; D007781:Lactoferrin; D002118:Calcium