title: Psychroflexus aestuariivivens sp. nov., isolated from a tidal flat.
abstract: A Gram-stain-negative, aerobic, non-flagellated, non-gliding and ovoid or rod-shaped bacterium, designated DB-3T, was isolated from a tidal flat on the Yellow Sea in South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain DB-3T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain DB-3T fell within the clade comprising the type strains of species of the genus Psychroflexus. Strain DB-3T exhibited 16S rRNA gene sequence similarities of 93.2-96.9 % to the type strains of species of the genus Psychroflexus. Strain DB-3T contained MK-6 as the predominant menaquinone and iso-C15:0, anteiso-C15:0 and iso-C17:0 3-OH as the major fatty acids. The major or significant amounts of polar lipids detected in strain DB-3T were phosphatidylethanolamine, an unidentified aminolipid and seven unidentified lipids. The DNA G+C content was 34.7 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain DB-3T is separated from recognized species of the genus Psychroflexus. On the basis of the data presented, strain DB-3T is considered to represent a novel species of the genus Psychroflexus, for which the name Psychroflexus aestuariivivens sp. nov. is proposed. The type strain is DB-3T (=KCTC 52037T=NBRC 111757T).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, South Korea.|University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, South Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), Yuseong, Daejeon, South Korea.
authors.forename: Song-Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, South Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6