title: Improved Method for the Qualitative Analyses of Palm Oil Carotenes Using UPLC.
abstract: Palm oil is the richest source of natural carotenes, comprising 500-700 ppm in crude palm oil (CPO). Its concentration is found to be much higher in oil extracted from palm-pressed fiber, a by-product from the milling of oil palm fruits. There are 11 types of carotenes in palm oil, excluding the cis/trans isomers of some of the carotenes. Qualitative separation of these individual carotenes is particularly useful for the identification and confirmation of different types of oil as the carotenes profile is unique to each type of vegetable oil. Previous studies on HPLC separation of the individual palm carotenes reported a total analyses time of up to 100 min using C30 stationary phase. In this study, the separation was completed in <5 min. The qualitative separation was successfully carried out using a commonly used stationary phase, C18.
authors.affiliation: Engineering and Processing Research Division, Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia ngmh@yahoo.com.
authors.forename: Mei Han
authors.initials: MH
authors.lastname: Ng
authors.affiliation: Engineering and Processing Research Division, Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.
authors.forename: Yuen May
authors.initials: YM
authors.lastname: Choo
mesh_terms: D002338:Carotenoids / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D010938:Plant Oils; D002338:Carotenoids; D000073878:Palm Oil