title: CD30 Receptor-Targeted Lentiviral Vectors for Human Induced Pluripotent Stem Cell-Specific Gene Modification.
abstract: Cultures of induced pluripotent stem cells (iPSCs) often contain cells of varying grades of pluripotency. We present novel lentiviral vectors targeted to the surface receptor CD30 (CD30-LV) to transfer genes into iPSCs that are truly pluripotent as demonstrated by marker gene expression. We demonstrate that CD30 expression is restricted to SSEA4(high) cells of human iPSC cultures and a human embryonic stem cell line. When CD30-LV was added to iPSCs during routine cultivation, efficient and exclusive transduction of cells positive for the pluripotency marker Oct-4 was achieved, while retaining their pluripotency. When added during the reprogramming process, CD30-LV solely transduced cells that became fully reprogrammed iPSCs as confirmed by co-expression of endogenous Nanog and the reporter gene. Thus, CD30-LV may serve as novel tool for the selective gene transfer into PSCs with broad applications in basic and therapeutic research.
authors.affiliation: 1 Molecular Biotechnology and Gene Therapy, Paul-Ehrlich-Institut , Langen, Germany .
authors.forename: Thorsten
authors.initials: T
authors.lastname: Friedel
authors.affiliation: 2 Medical Biotechnology, Paul-Ehrlich-Institut , Langen, Germany .
authors.forename: Sabine
authors.initials: S
authors.lastname: Jung-Klawitter
authors.affiliation: 2 Medical Biotechnology, Paul-Ehrlich-Institut , Langen, Germany .
authors.forename: Attila
authors.initials: A
authors.lastname: Sebe
authors.affiliation: 3 Research Group for Gene Modification in Stem Cells, LOEWE Center of Cell and Gene Therapy Frankfurt , Paul-Ehrlich-Institut, Langen, Germany .
authors.forename: Franziska
authors.initials: F
authors.lastname: Schenk
authors.affiliation: 3 Research Group for Gene Modification in Stem Cells, LOEWE Center of Cell and Gene Therapy Frankfurt , Paul-Ehrlich-Institut, Langen, Germany .
authors.forename: Ute
authors.initials: U
authors.lastname: Modlich
authors.affiliation: 2 Medical Biotechnology, Paul-Ehrlich-Institut , Langen, Germany .
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Ivics
authors.affiliation: 2 Medical Biotechnology, Paul-Ehrlich-Institut , Langen, Germany .
authors.forename: Gerald G
authors.initials: GG
authors.lastname: Schumann
authors.affiliation: 1 Molecular Biotechnology and Gene Therapy, Paul-Ehrlich-Institut , Langen, Germany .
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Buchholz
authors.affiliation: 1 Molecular Biotechnology and Gene Therapy, Paul-Ehrlich-Institut , Langen, Germany .
authors.forename: Irene C
authors.initials: IC
authors.lastname: Schneider
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D065150:Cellular Reprogramming; D002999:Clone Cells; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D017730:Ki-1 Antigen / Q000378:metabolism*; D016086:Lentivirus / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D015415:Biomarkers; D017730:Ki-1 Antigen