title: Genetic variants at 18q11.2 and 8q24 identified by genome-wide association studies were not associated with pulmonary tuberculosis risk in Chinese population.
abstract: Genome-wide association study (GWAS) recently identified several susceptibility loci in ASAP1 gene on chromosome 8q24 for tuberculosis (TB) in a Russian population, but no relevant studies have been performed to validate these findings. In addition, previous GWAS in Ghana and Gambia found that the variant rs4331426 at 18q11.2 was a susceptibility locus for TB. However, the follow-up studies reported conflicting results. Herein, we investigated the contribution of genetic variants at 8q24 and 18q11.2 to TB in Chinese population.
  
  
  
  We genotyped four genetic variants at 8q24 (rs10956514 and rs11774633) and 18q11.2 (rs4331426 and rs6507226) in a case-control study with 355 newly bacteriologically confirmed pulmonary TB cases and 395 healthy controls using TaqMan allelic discrimination assay. Subsequently, we conducted a meta-analysis including 4 reported studies in Chinese populations and our case-control study with a total of 3118 cases and 3226 controls to further evaluate the relationship between rs4331426 at 18q11.2 and TB risk.
  
  
  
  We did not find significant association between genetic variants at 8q24 and risk of TB (rs10956514: OR=0.89, 95%CI: 0.72-1.09, P=0.253; rs11774633: OR=0.86, 95%CI: 0.69-1.08, P=0.206). We did not observe significant association for genetic variants at 18q11.2 (rs4331426: OR=0.62, 95%CI: 0.34-1.14, P=0.122; and rs6507226: OR=0.98, 95%CI: 0.80-1.20, P=0.853). Moreover, the pooled results from the Meta-analysis further supported that rs4331426 at 18q11.2 was not associated with TB risk in Chinese population (OR=0.90, 95% CI: 0.63-1.29).
  
  
  
  Our findings indicate that TB risk-associated loci at 8q24 and 18q11.2 identified by GWAS from the other populations may not contribute to TB susceptibility in Chinese population.
authors.affiliation: Department of Chronic Communicable Disease, Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, China. Electronic address: ruifenmiao@126.com.
authors.forename: Ruifen
authors.initials: R
authors.lastname: Miao
authors.affiliation: Nanjing Chest Hospital, Nanjing 210029, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Ge
authors.affiliation: Nanjing Chest Hospital, Nanjing 210029, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Chronic Communicable Disease, Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, China.
authors.forename: Zhaoping
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Chronic Communicable Disease, Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Chronic Communicable Disease, Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Chronic Communicable Disease, Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, China.
authors.forename: Songning
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Chronic Communicable Disease, Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Chronic Communicable Disease, Nanjing Municipal Center for Disease Control and Prevention, Nanjing 210003, China. Electronic address: frankxufei@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002887:Chromosomes, Human, Pair 18; D002898:Chromosomes, Human, Pair 8; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011159:Population Surveillance; D040641:Quantitative Trait Loci; D018570:Risk Assessment; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000453:epidemiology* / Q000235:genetics* / Q000382:microbiology
keywords: ASAP1; GWAS; Polymorphism; Tuberculosis