title: Human Papillomavirus E2 Regulates SRSF3 (SRp20) To Promote Capsid Protein Expression in Infected Differentiated Keratinocytes.
abstract: The human papillomavirus (HPV) life cycle is tightly linked to differentiation of the infected epithelial cell, suggesting a sophisticated interplay between host cell metabolism and virus replication. Previously, we demonstrated in differentiated keratinocytes in vitro and in vivo that HPV type 16 (HPV16) infection caused increased levels of the cellular SR splicing factors (SRSFs) SRSF1 (ASF/SF2), SRSF2 (SC35), and SRSF3 (SRp20). Moreover, the viral E2 transcription and replication factor that is expressed at high levels in differentiating keratinocytes could bind and control activity of the SRSF1 gene promoter. Here, we show that the E2 proteins of HPV16 and HPV31 control the expression of SRSFs 1, 2, and 3 in a differentiation-dependent manner. E2 has the greatest transactivation effect on expression of SRSF3. Small interfering RNA depletion experiments in two different models of the HPV16 life cycle (W12E and NIKS16) and one model of the HPV31 life cycle (CIN612-9E) revealed that only SRSF3 contributed significantly to regulation of late events in the virus life cycle. Increased levels of SRSF3 are required for L1 mRNA and capsid protein expression. Capsid protein expression was regulated specifically by SRSF3 and appeared independent of other SRSFs. Taken together, these data suggest a significant role of the HPV E2 protein in regulating late events in the HPV life cycle through transcriptional regulation of SRSF3 expression.
  
  
  
  Human papillomavirus replication is accomplished in concert with differentiation of the infected epithelium. Virus capsid protein expression is confined to the upper epithelial layers so as to avoid immune detection. In this study, we demonstrate that the viral E2 transcription factor activates the promoter of the cellular SRSF3 RNA processing factor. SRSF3 is required for expression of the E4(^)L1 mRNA and so controls expression of the HPV L1 capsid protein. Thus, we reveal a new dimension of virus-host interaction crucial for production of infectious virus. SRSF proteins are known drug targets. Therefore, this study provides an excellent basis for developing strategies to regulate capsid protein production in the infected epithelium and the production of new virions.
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity, and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.
authors.forename: T
authors.initials: T
authors.lastname: Klymenko
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity, and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.
authors.forename: H
authors.initials: H
authors.lastname: Hernandez-Lopez
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity, and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.
authors.forename: A I
authors.initials: AI
authors.lastname: MacDonald
authors.affiliation: Louisiana State University Health Sciences Center, Department of Microbiology and Immunology, Shreveport, Louisiana, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Bodily
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Immunity, and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom sheila.graham@gla.ac.uk.
authors.forename: S V
authors.initials: SV
authors.lastname: Graham
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D052162:Human papillomavirus 16 / Q000235:genetics; D058952:Human papillomavirus 31 / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology / Q000821:virology*; D008018:Life Cycle Stages / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism*; D027383:Papillomaviridae / Q000235:genetics* / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D000068103:Serine-Arginine Splicing Factors / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; C000595779:SRSF1 protein, human; C491388:SRSF3 protein, human; D014157:Transcription Factors; C076036:oncogene protein E2, Human papillomavirus type 1; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors