title: Outbreak Caused by blaOXA-72-Producing Acinetobacter baumannii ST417 Detected in Clinical and Environmental Isolates.
abstract: We characterized an outbreak of imipenem-resistant Acinetobacter baumannii with clinical and environmental isolates from a tertiary care hospital in San Luis Potosi, Mexico. During a 4-month period, a total of 32 nonrepetitive imipenem-resistant clinical isolates of A. baumannii were collected. All isolates were susceptible to colistin and tigecycline and resistant to cefepime, ceftazidime, ceftriaxone, imipenem, and meropenem. Genotyping by pulsed-field gel electrophoresis showed a major clone (A). Multilocus sequence type (MLST) analysis was performed, revealing sequence type (ST) 417 (ST417) and 208 (ST208). The blaIMP-, blaVIM-, blaGIM-, blaSIM-, blaNDM-type, and blaOXA-type (blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and blaOXA-58-like) genes were screened and showed that the blaOXA-51-like and blaOXA-24-like genes were present in all isolates. Sequencing and southern hybridization were performed, confirming the presence of the blaOXA-72 gene and its plasmid-borne nature. In addition, the blaOXA-72-XerC/XerD-like association was identified. These findings indicate that a clonal spread of blaOXA-72-producing A. baumannii ST417 had occurred throughout the hospital. The ST417 corresponded with a previous ST described in the United States.
authors.affiliation: 1 Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública , Cuernavaca, México.
authors.forename: Elsa
authors.initials: E
authors.lastname: Tamayo-Legorreta
authors.affiliation: 2 Laboratorio de Genética y Diagnóstico Molecular and Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí , San Luis Potosí, México.
authors.forename: Edgar
authors.initials: E
authors.lastname: Turrubiartes-Martínez
authors.affiliation: 1 Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública , Cuernavaca, México.
authors.forename: Ulises
authors.initials: U
authors.lastname: Garza-Ramos
authors.affiliation: 2 Laboratorio de Genética y Diagnóstico Molecular and Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí , San Luis Potosí, México.
authors.forename: Perla
authors.initials: P
authors.lastname: Niño-Moreno
authors.affiliation: 1 Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública , Cuernavaca, México.
authors.forename: Humberto
authors.initials: H
authors.lastname: Barrios
authors.affiliation: 1 Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública , Cuernavaca, México.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez-Pérez
authors.affiliation: 1 Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública , Cuernavaca, México.
authors.forename: Fernando
authors.initials: F
authors.lastname: Reyna-Flores
authors.affiliation: 3 Laboratorio de Microbiología, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí , México.
authors.forename: Juana
authors.initials: J
authors.lastname: Tovar-Oviedo
authors.affiliation: 4 Hospital Central Dr. Ignacio Morones Prieto , San Luis Potosí, México.
authors.forename: Martin
authors.initials: M
authors.lastname: Magaña-Aquino
authors.affiliation: 5 Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México , Cuernavaca, México.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Cevallos
authors.affiliation: 1 Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública , Cuernavaca, México.
authors.forename: Jesus
authors.initials: J
authors.lastname: Silva-Sanchez
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003091:Colistin / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008800:Mexico / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000031:analogs & derivatives / Q000494:pharmacology; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D062606:Tertiary Care Centers; D000078304:Tigecycline; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007527:Isoenzymes; D047090:beta-Lactams; D000078304:Tigecycline; D001618:beta-Lactamases; D008911:Minocycline; D003091:Colistin