title: Paraventricular nucleus is involved in the central pathway of adipose afferent reflex in rats.
abstract: Increasing evidence indicates a link between sympathetic nervous system activation and obesity, but the underlying mechanisms remain elusive. The adipose afferent reflex (AAR) is a sympathoexcitatory reflex that is activated by afferent neurotransmission from the white adipose tissue (WAT). This study aimed to investigate whether the hypothalamic paraventricular nucleus (PVH) is an important component of the central neurocircuitry of the AAR. In anesthetized rats, the discharge activity of individual PVH neurons was recorded in vivo. Activation of WAT afferents was initiated by capsaicin injection, and the AAR was evaluated by monitoring renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) responses. The responses of PVH neurons to activation of WAT afferents were evaluated by c-fos immunoreactivity and the discharge activity of individual PVH neurons, which was recorded using extracellular single-unit recording. After activation of WAT afferents, both individual PVH neuron discharge activity and c-fos immunoreactivity increased. Bilateral selective lesions of the neurons in the PVH with kainic acid abolished the AAR. These results indicate that PVH is an important component of the central neurocircuitry of the AAR.
authors.affiliation: a Department of Physiology, Binzhou Medical University, 346 Guanhai Rd., Laishan District, Yantai 264003, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shi
authors.affiliation: b Department of Pharmacology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Yuan-Fang
authors.initials: YF
authors.lastname: Wang
authors.affiliation: a Department of Physiology, Binzhou Medical University, 346 Guanhai Rd., Laishan District, Yantai 264003, China.
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Wang
authors.affiliation: c Department of Pathogenic Biology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Wu
authors.affiliation: a Department of Physiology, Binzhou Medical University, 346 Guanhai Rd., Laishan District, Yantai 264003, China.|d Shandong Province Key Laboratory of Stroke.
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Ma
mesh_terms: D052436:Adipose Tissue, White / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D002211:Capsaicin / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D007264:Inguinal Canal; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D008297:Male; D008959:Models, Neurological; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012187:Retroperitoneal Space; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D015415:Biomarkers; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D002211:Capsaicin; D007608:Kainic Acid
keywords: activité sympathique; adipose afferent reflex; blood pressure; electrophysiology; enregistrement extracellulaire unitaire; extracellular single-unit recording; noyau paraventriculaire; paraventricular nucleus; pression sanguine; réflexe afférent adipeux; sympathetic activity; tissu adipeux blanc; white adipose tissue; électrophysiologie