title: Dp71Δ78-79 dystrophin mutant stimulates neurite outgrowth in PC12 cells via upregulation and phosphorylation of HspB1.
abstract: PC12 cells acquire a neuronal phenotype in response to nerve growth factor (NGF). However, this phenotype is more efficiently achieved when the Dp71Δ78-79 dystrophin mutant is stably expressed in PC12-C11 cells. To investigate the effect of Dp71Δ78-79 overexpression on the protein profile of PC12-C11 cells, we compared the expression profiles of undifferentiated and NGF-differentiated PC12-C11 and PC12 cells by 2DE. In undifferentiated cultures, one protein was downregulated, and five were upregulated. Dp71Δ78-79 overexpression had a greater effect on differentiated cultures, with ten proteins downregulated and seven upregulated. The protein with the highest upregulation was HspB1. Changes in HspB1 expression were validated by Western blot and immunofluorescence analyses. Interestingly, the neurite outgrowth in PC12-C11 cells was affected by a polyclonal antibody against HspB1, and the level of HspB1 and HspB1Ser86 decreased, suggesting an important role for this protein in this cellular process. Our results show that Dp71Δ78-79 affects the expression level of some proteins and that the stimulated neurite outgrowth produced by this mutant is mainly through upregulation and phosphorylation of HspB1.
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F., México.
authors.forename: Candelaria
authors.initials: C
authors.lastname: Merino-Jiménez
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F., México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Aragón
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F., México.
authors.forename: Víctor
authors.initials: V
authors.lastname: Ceja
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F., México.
authors.forename: Griselda
authors.initials: G
authors.lastname: Rodríguez-Martínez
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F., México.
authors.forename: Febe E
authors.initials: FE
authors.lastname: Cázares-Raga
authors.affiliation: UPMC Univ Paris 06, UMS 2 Omique, Sorbonne Universités, Plateforme P3S, Paris, France.|UMS 29 Omique, INSERM, Plateforme P3S, Paris, France.
authors.forename: Solenne
authors.initials: S
authors.lastname: Chardonnet
authors.affiliation: UPMC Univ Paris 06, UMS 2 Omique, Sorbonne Universités, Plateforme P3S, Paris, France.|UMS 29 Omique, INSERM, Plateforme P3S, Paris, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Pionneau
authors.affiliation: Institut de la Vision, INSERM UMR_S968, CNRS UMR_7210, Université Pierre et Marie Curie Paris 06, Paris, France.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Rendon
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F., México.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Montañez
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D016189:Dystrophin / Q000235:genetics* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D018832:Molecular Chaperones; D009154:Mutation; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D020932:Nerve Growth Factor / Q000494:pharmacology; D000071446:Neuronal Outgrowth / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D010766:Phosphorylation; D051381:Rats; D015398:Signal Transduction
chemical_list: D000906:Antibodies; D016189:Dystrophin; D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D020932:Nerve Growth Factor
keywords: Cell biology; Dp71Δ78-79; HspB1; MS; Neurite outgrowth; PC12 cells