title: Influence of experimental Anaplasma marginale infection and splenectomy on NTPDase and 5'nucleotidase activities in platelets of cattle.
abstract: The objective of this paper was to evaluate NTPDase and 5'-nucleotidase activities in platelets of bovine with and without spleen and infected by Anaplasma marginale. Our results demonstrate that infection along with splenectomy is able of inducing a profile of cellular protection, which showed an increase in the degradation of the nucleotides ATP and ADP by NTPDase, in addition to AMP by 5'nucleotidase to form the nucleoside adenosine in platelets, i.e., the enzymatic activities of platelets were increased in splenectomized animals when compared to non-splenectomized group. It notes that adenosine is a molecule with anti-inflammatory function. But this profile is related to a deficiency in immune signaling triggered by nucleotide ATP, which may be related to the increase in bacteremia and disability in combating the parasite in splenectomized host.
authors.affiliation: Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Instituto de Pesquisas Veterinárias Desidério Finamor, FEPAGRO, Health Animal Sciences, Eldorado do Sul, RS, Brazil. Electronic address: rovainadoyle@yahoo.com.
authors.forename: Rovaina L
authors.initials: RL
authors.lastname: Doyle
authors.affiliation: Graduate Program in Veterinary Medicine, Department of Microbiology and Parasitology, Prédio 20, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Camila B
authors.initials: CB
authors.lastname: Oliveira
authors.affiliation: Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Raqueli T
authors.initials: RT
authors.lastname: França
authors.affiliation: Graduate Program in Toxicological Biochemistry, Department of Microbiology and Parasitology, Prédio 20, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Pedro H
authors.initials: PH
authors.lastname: Doleski
authors.affiliation: Graduate Program in Toxicological Biochemistry, Department of Microbiology and Parasitology, Prédio 20, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Viviane C
authors.initials: VC
authors.lastname: Souza
authors.affiliation: Graduate Program in Toxicological Biochemistry, Department of Microbiology and Parasitology, Prédio 20, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Daniela B R
authors.initials: DBR
authors.lastname: Leal
authors.affiliation: Instituto de Pesquisas Veterinárias Desidério Finamor, FEPAGRO, Health Animal Sciences, Eldorado do Sul, RS, Brazil.
authors.forename: João R
authors.initials: JR
authors.lastname: Martins
authors.affiliation: Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Sonia T A
authors.initials: STA
authors.lastname: Lopes
authors.affiliation: Veterinary Epidemiology Laboratory, Department of Preventive Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Machado
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, SC, Brazil.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.affiliation: Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Cinthia M
authors.initials: CM
authors.lastname: Andrade
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis*; D000241:Adenosine / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000032:analysis*; D042322:Anaplasma marginale / Q000254:growth & development*; D000712:Anaplasmosis / Q000473:pathology*; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology*; D002417:Cattle; D004195:Disease Models, Animal; D057131:Immune Evasion; D007108:Immune Tolerance; D013156:Splenectomy
chemical_list: D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D000241:Adenosine
keywords: Anaplasma marginale; Ectonucleotidase; Splenectomized