title: Association of single nucleotide polymorphisms in CAPN1, CAST and MB genes with meat color of Brahman and crossbreed cattle.
abstract: The objective of this research was to determine the association of SNPs in the candidate genes Calpain (CAPN1), Calpastatin (CAST) and Myoglobin (MB) with colorimetric parameters (L *, a *, b *, C *, hue) in a F1 population (n = 164) obtained from crossing Bos taurus × Bos indicus and Bos indicus × Bos indicus. SNPs were analyzed using PCR-RFLP and SSCP. Colorimetric measurements were performed in the muscles Longissimus thoracis et lumborum (LTL) and Semitendinosus (ST) at 7, 14 and 21 days postmortem applying the methodology CIE L* a* b*. The CAST gene showed a significant effect on the b* and hue* parameters in both muscles. MB gene showed significant association with all colorimetric parameters in both LTL and ST muscles, except with b* parameter. The CAPN1 gene did not show any significant association. These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions.
authors.affiliation: Universidad de Cundinamarca, Animal Science Faculty, Fusagasugá, Campus, Diagonal 18 No. 20-29, Colombia; Molecular Animal Genetics Group, Veterinary and Animal Science Faculty, Universidad Nacional de Colombia, Bogotá Campus, A.A 76948, Colombia. Electronic address: sulcastromo@unal.edu.co.
authors.forename: Susan
authors.initials: S
authors.lastname: Castro
authors.affiliation: Molecular Animal Genetics Group, Veterinary and Animal Science Faculty, Universidad Nacional de Colombia, Bogotá Campus, A.A 76948, Colombia.
authors.forename: Marcela
authors.initials: M
authors.lastname: Ríos
authors.affiliation: Molecular Animal Genetics Group, Veterinary and Animal Science Faculty, Universidad Nacional de Colombia, Bogotá Campus, A.A 76948, Colombia.
authors.forename: Yurany
authors.initials: Y
authors.lastname: Ortiz
authors.affiliation: Molecular Animal Genetics Group, Veterinary and Animal Science Faculty, Universidad Nacional de Colombia, Bogotá Campus, A.A 76948, Colombia.
authors.forename: Carlos
authors.initials: C
authors.lastname: Manrique
authors.affiliation: Asociación Colombiana de Criadores de Ganado Cebú (ASOCEBU), Transversal 21 N 95-64, Santafé de Bogotá, Colombia.
authors.forename: Ariel
authors.initials: A
authors.lastname: Jiménez
authors.affiliation: Molecular Animal Genetics Group, Veterinary and Animal Science Faculty, Universidad Nacional de Colombia, Bogotá Campus, A.A 76948, Colombia.
authors.forename: Fernando
authors.initials: F
authors.lastname: Ariza
mesh_terms: D000818:Animals; D002154:Calpain / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D003433:Crosses, Genetic; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D008460:Meat / Q000032:analysis*; D009211:Myoglobin / Q000235:genetics / Q000378:metabolism*; D010858:Pigmentation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D003598:Cytoskeletal Proteins; D009211:Myoglobin; D002154:Calpain; C484807:CAPN1 protein, Bos taurus
keywords: Alleles; CAPN1; CAST; MB; Meat color; SNPs