title: Altered expression of micro-RNA 199a and increased levels of cardiac SIRT1 protein are associated with the occurrence of atrial fibrillation after coronary artery bypass graft surgery.
abstract: BACKGROUND
  Postoperative atrial fibrillation (POAF) is a potentially life-threatening complication after coronary artery bypass graft (CABG) surgery. The expression of the cardioprotective SIRT1 protein with its antioxidant activity is increased in cardiac tissue of patients suffering from POAF. So far, information is lacking about the relationship between SIRT1 regulating micro RNAs (miRs), SIRT1 protein and the occurrence of POAF.
  
  
  METHODS
  A total of 63 patients undergoing CABG were recruited, and biopsies were obtained from the right atrial appendage during cannulation. Postoperative, all patients were rhythm-monitored until discharge and randomized to POAF (n=20) or sinus rhythm (n=43). The expression of the micro RNAs miR-199a and miR-195 was quantified by real-time PCR. SIRT1 protein was detected by western blot analysis.
  
  
  RESULTS
  The relative expression of miR-199a in the POAF group was significantly decreased compared to the control group (0.77±0.27 vs. 1.11±0.69, P=.022) Accordingly, SIRT 1 protein was significantly induced in tissue probes of patients with POAF (P<.001).
  
  
  CONCLUSIONS
  Altered expression of the SIRT1 protein regulating miR-199a in human atrial tissue was found to be related to the occurrence of POAF, indicating its usefulness as a biomarker for cardiac surgery management.
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Cardiology, Istanbul, Turkey.
authors.forename: Aylin Hatice
authors.initials: AH
authors.lastname: Yamac
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Cardiology, Istanbul, Turkey.
authors.forename: Sitki
authors.initials: S
authors.lastname: Kucukbuzcu
authors.affiliation: Istanbul Medipol University, Faculty of Medicine, Department of Physiology and Regenerative and Restorative Medicine Research Center (REMER), Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozansoy
authors.affiliation: Istanbul Medipol University, Vocational School of Health Services, Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Gok
authors.affiliation: Mehmet Akif Ersoy Heart Hospital, Department of Cardiovascular Surgery, Istanbul, Turkey.
authors.forename: Kursat
authors.initials: K
authors.lastname: Oz
authors.affiliation: Mehmet Akif Ersoy Heart Hospital, Department of Cardiology, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Erturk
authors.affiliation: Mehmet Akif Ersoy Heart Hospital, Department of Cardiology, Istanbul, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Yilmaz
authors.affiliation: Mehmet Akif Ersoy Heart Hospital, Department of Cardiovascular Surgery, Istanbul, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Ersoy
authors.affiliation: Bezmialem Vakif University, Department of Cardiovascular Surgery, Istanbul, Turkey.
authors.forename: Rahmi
authors.initials: R
authors.lastname: Zeybek
authors.affiliation: Bezmialem Vakif University, Faculty of Medicine, Department of Cardiology, Istanbul, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Goktekin
authors.affiliation: Istanbul Medipol University, Faculty of Medicine, Department of Medical Biology and Regenerative and Restorative Medicine Research Center (REMER), Istanbul, Turkey. Electronic address: uckilic@yahoo.com.
authors.forename: Ulkan
authors.initials: U
authors.lastname: Kilic
mesh_terms: D000368:Aged; D001281:Atrial Fibrillation / Q000209:etiology* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D001026:Coronary Artery Bypass / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism; D011183:Postoperative Complications / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C531408:mirn199 microRNA, human; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Postoperative atrial fibrillation; SIRT 1 protein; miR-199a