title: Analysis of Genetic Diversity and Development of SCAR Markers in a Mycogone perniciosa Population.
abstract: The fungus Mycogone perniciosa is a major pathogen of the common button mushroom Agaricus bisporus. Analysis of genetic diversity in M. Perniciosa may assist in developing methods for prophylaxis and treatment of M. Perniciosa infections. For this, it is necessary to classify M. Perniciosa into relevant class groups quickly and efficiently. Random amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR), and sequence-related amplified polymorphism (SRAP) markers were used to obtain genetic fingerprints and assess the genetic variation among 49 strains of M. perniciosa collected from different areas of Fujian Province in China. Analysis of DNA sequence polymorphism revealed two major distinct groups (Group I and Group II). Specific DNA fragments that were identified through RAPD, ISSR, and SRAP markers were sequenced and used for the designing of stable sequence-characterized amplified region (SCAR) markers. The resulting SCAR markers were then validated against the classified groups of M. perniciosa.
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.|College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Bingzhi
authors.initials: B
authors.lastname: Chen
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Chenghuan
authors.initials: C
authors.lastname: Li
authors.affiliation: Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. zwenfafu@163.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Wen
mesh_terms: D000364:Agaricus / Q000737:chemistry; D004271:DNA, Fungal / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D006999:Hypocreales / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique; D014675:Vegetables / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D005819:Genetic Markers