title: Heterogeneity in resistant fecal Bacteroides fragilis group collected from healthy people.
abstract: Normal nonpathogenic flora would represent a constant lake of resistance genes potentially transferable to human pathogens. To assess the prevalence of resistance genes and genetic variability of Bacteroides fragilis group (BFG) from normal flora, 177 Bacteroides isolates obtained from the fecal samples of healthy individuals. These isolates were subjected to antibiotic susceptibility testing and pulsed field gel electrophoresis (PFGE). The isolates were further tested for the presence of ermF, tetQ and bft genes by PCR. Our results indicated the presence of different clonal strains (1 common type and 57 single types) among the resistant isolates. The resistance rate for the six antibiotics in this study was between 1% and 95%. Most of the isolates (99%) were susceptible to metronidazole. ermF and tetQ were detected in all erythromycin and tetracycline resistant isolates. None of the isolates were carried bft gene. These data suggest dissemination of heterogenic clonal groups in healthy persons and resistance to 5 high commonly used antibiotics.
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: T
authors.initials: T
authors.lastname: Narimani
authors.affiliation: Department of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Douraghi
authors.affiliation: Molecular Microbiology Research Center(MMRC), Shahed University, Tehran, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Owlia
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Rastegar
authors.affiliation: Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Esghaei
authors.affiliation: Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Nasr
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address: talebi.m@iums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Talebi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001441:Bacteroides fragilis / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology*; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D064368:Healthy Volunteers; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents
keywords: Bacteroides; Erythromycin; PFGE; Tetracycline; ermF; tetQ