title: Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment.
abstract: A halotolerant, Gram-stain-negative, rod-shaped and light-pink-pigmented bacterial strain, PB3T, was isolated from a pond sediment near a hexachlorocyclohexane-producing factory, located at Chinhat, Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PB3T formed a distinct phyletic clade along with the members of the genus Pontibacter. The 16S rRNA gene sequence similarity with other members of the genus Pontibacter ranged from 94.5 to 98.9 %. The cells were motile, aerobic, and catalase- and oxidase-positive. The major fatty acids were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C16:1ω5c, summed feature 3 (C16:1ω6c/C16:1ω7c) and summed feature 4 (iso-C17:1I/ anteiso-C17:1 B). The polar lipid profile of strain PB3T showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids and other unknown polar lipids. DNA-DNA hybridization based homology of strain PB3T with respect to its most closely related species, Pontibacter chinhatensis LP51T, was 44.7 %. The DNA G+C content was 53.5 mol%. On the basis of these data, it is proposed that the isolate belongs to the genus Pontibacter and represents a novel species, for which the name Pontibacter mucosus is proposed. The type strain is PB3T (=DSM 100162T=KCTC 42942T).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Namita
authors.initials: N
authors.lastname: Nayyar
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Puneet
authors.initials: P
authors.lastname: Kohli
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Nitish Kumar
authors.initials: NK
authors.lastname: Mahato
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
authors.forename: Rup
authors.initials: R
authors.lastname: Lal
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D001556:Hexachlorocyclohexane / Q000032:analysis*; D007194:India; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014873:Water Pollutants / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D014873:Water Pollutants; C483858:phosphatidylethanolamine; D001556:Hexachlorocyclohexane