title: Ultrasound biomicroscopy and claudication test for in vivo follow-up of muscle repair enhancement based on platelet-rich plasma therapy in a rat model of gastrocnemius laceration.
abstract: OBJECTIVE
  To track the regeneration process of lateral gastrocnemius due to a muscle laceration in rats, and to treatment with plateletrich plasma (PRP).
  
  
  METHODS
  Ultrasound (40 MHz) images were used for measuring pennation angle (PA), muscle thickness (MT) and mean pixel intensity, along with claudication scores, of treated (PRPG) and non-treated (NTG) groups of rats.
  
  
  RESULTS
  NTG showed a PA increase for the non-injured leg (p<0.05) and a tendency of MT to increase, whereas for PRPG there were no differences. There was a progressive reduction of the claudication score for the PRPG group throughout the entire period, with an immediate difference after seven days (p<0.05), whereas the NTG had a significant reduction only at day 28 (p<0.05).
  
  
  CONCLUSIONS
  It was observed a compensatory hypertrophic response due to the overload condition imposed to healthy leg for NTG that did not occur in PRPG, suggesting an accelerated repair process of the injured leg due to treatment, anticipating its use.
authors.affiliation: Department of Surgery, School of Medicine, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Cristiane Lima
authors.initials: CL
authors.lastname: Pinheiro
authors.affiliation: COPPE, UFRJ, Rio de Janeiro, RJ, Brazil.
authors.forename: Carolina Carneiro
authors.initials: CC
authors.lastname: Peixinho
authors.affiliation: Department of Surgery, School of Medicine, UFRJ, Rio de Janeiro, RJ, Brazil.
authors.forename: Cristiano Costa
authors.initials: CC
authors.lastname: Esposito
authors.affiliation: Department of Surgery, School of Medicine, UFRJ, Rio de Janeiro, RJ, Brazil.
authors.forename: José Eduardo Ferreira
authors.initials: JE
authors.lastname: Manso
authors.affiliation: School of Medicine and Biomedical Engineering Program, UFRJ, Rio de Janeiro, RJ, Brazil.
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Machado
mesh_terms: D000818:Animals; D005260:Female; D005500:Follow-Up Studies; D007383:Intermittent Claudication / Q000628:therapy; D000069416:Intravital Microscopy / Q000379:methods; D022125:Lacerations / Q000000981:diagnostic imaging / Q000628:therapy*; D033401:Microscopy, Acoustic / Q000379:methods; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000000981:diagnostic imaging / Q000502:physiology* / Q000503:physiopathology*; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*