title: Efficient Conversion of Inulin to Inulooligosaccharides through Endoinulinase from Aspergillus niger.
abstract: Inulooligosaccharides (IOS) represent an important class of oligosaccharides at industrial scale. An efficient conversion of inulin to IOS through endoinulinase from Aspergillus niger is presented. A 1482 bp codon optimized gene fragment encoding endoinulinase from A. niger DSM 2466 was cloned into pPIC9K vector and was transformed into Pichia pastoris KM71. Maximum activity of the recombinant endoinulinase, 858 U/mL, was obtained at 120 h of the high cell density fermentation process. The optimal conditions for inulin hydrolysis using the recombinant endoinulinase were investigated. IOS were harvested with a high concentration of 365.1 g/L and high yield up to 91.3%. IOS with different degrees of polymerization (DP, mainly DP 3-6) were distributed in the final reaction products.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhaojuan
authors.initials: Z
authors.lastname: Zheng
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yong
authors.affiliation: Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education , Nanjing, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry* / Q000201:enzymology; D003001:Cloning, Molecular; D003062:Codon; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007444:Inulin / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000378:metabolism*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D003062:Codon; D005656:Fungal Proteins; D009844:Oligosaccharides; D011994:Recombinant Proteins; D007444:Inulin; D006026:Glycoside Hydrolases; C000612463:endoinulinase, Aspergillus niger
keywords: Aspergillus niger; Pichia pastoris; endoinulinase; inulin; inulooligosaccharide