title: Evolutionary diversification of the BetaM interactome acquired through co-option of the ATP1B4 gene in placental mammals.
abstract: ATP1B4 genes represent a rare instance of orthologous vertebrate gene co-option that radically changed properties of the encoded BetaM proteins, which function as Na,K-ATPase subunits in lower vertebrates and birds. Eutherian BetaM has lost its ancestral function and became a muscle-specific resident of the inner nuclear membrane. Our earlier work implicated BetaM in regulation of gene expression through direct interaction with the transcriptional co-regulator SKIP. To gain insight into evolution of BetaM interactome we performed expanded screening of eutherian and avian cDNA libraries using yeast-two-hybrid and split-ubiquitin systems. The inventory of identified BetaM interactors includes lamina-associated protein LAP-1, myocyte nuclear envelope protein Syne1, BetaM itself, heme oxidases HMOX1 and HMOX2; transcription factor LZIP/CREB3, ERGIC3, PHF3, reticulocalbin-3, and β-sarcoglycan. No new interactions were found for chicken BetaM and human Na,K-ATPase β1, β2 and β3 isoforms, indicating the uniqueness of eutherian BetaM interactome. Analysis of truncated forms of BetaM indicates that residues 72-98 adjacent to the membrane in nucleoplasmic domain are important for the interaction with SKIP. These findings demonstrate that evolutionary alterations in structural and functional properties of eutherian BetaM proteins are associated with the increase in its interactome complexity.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117871, Russia.|Department of Physiology and Pharmacology and Center for Diabetes and Endocrine Research, University of Toledo College of Medicine, 3000 Arlington Ave, Toledo, OH 43614, USA.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Korneenko
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117871, Russia.|Department of Physiology and Pharmacology and Center for Diabetes and Endocrine Research, University of Toledo College of Medicine, 3000 Arlington Ave, Toledo, OH 43614, USA.
authors.forename: Nikolay B
authors.initials: NB
authors.lastname: Pestov
authors.affiliation: Department of Physiology and Pharmacology and Center for Diabetes and Endocrine Research, University of Toledo College of Medicine, 3000 Arlington Ave, Toledo, OH 43614, USA.
authors.forename: Nisar
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117871, Russia.
authors.forename: Irina A
authors.initials: IA
authors.lastname: Okkelman
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117871, Russia.
authors.forename: Ruslan I
authors.initials: RI
authors.lastname: Dmitriev
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117871, Russia.
authors.forename: Mikhail I
authors.initials: MI
authors.lastname: Shakhparonov
authors.affiliation: Department of Physiology and Pharmacology and Center for Diabetes and Endocrine Research, University of Toledo College of Medicine, 3000 Arlington Ave, Toledo, OH 43614, USA.
authors.forename: Nikolai N
authors.initials: NN
authors.lastname: Modyanov
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D005075:Biological Evolution; D001717:Birds; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D014644:Genetic Variation; D050883:HSC70 Heat-Shock Proteins; D006801:Humans; D008322:Mammals; D009132:Muscles / Q000502:physiology*; D009685:Nuclear Envelope; D009928:Organ Specificity; D010802:Phylogeny; D011485:Protein Binding / Q000235:genetics; D049031:Sarcoglycans / Q000235:genetics / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: C585649:ATP1B4 protein, human; D048868:Adaptor Proteins, Signal Transducing; D002135:Calcium-Binding Proteins; D050883:HSC70 Heat-Shock Proteins; C494371:HSPA8 protein, human; C510564:RCN3 protein, human; C466999:SPHKAP protein, human; D049031:Sarcoglycans; D014157:Transcription Factors; D000254:Sodium-Potassium-Exchanging ATPase