title: Dynamic Phosphorylation of VP30 Is Essential for Ebola Virus Life Cycle.
abstract: Ebola virus is the causative agent of a severe fever with high fatality rates in humans and nonhuman primates. The regulation of Ebola virus transcription and replication currently is not well understood. An important factor regulating viral transcription is VP30, an Ebola virus-specific transcription factor associated with the viral nucleocapsid. Previous studies revealed that the phosphorylation status of VP30 impacts viral transcription. Together with NP, L, and the polymerase cofactor VP35, nonphosphorylated VP30 supports viral transcription. Upon VP30 phosphorylation, viral transcription ceases. Phosphorylation weakens the interaction between VP30 and the polymerase cofactor VP35 and/or the viral RNA. VP30 thereby is excluded from the viral transcription complex, simultaneously leading to increased viral replication which is supported by NP, L, and VP35 alone. Here, we use an infectious virus-like particle assay and recombinant viruses to show that the dynamic phosphorylation of VP30 is critical for the cotransport of VP30 with nucleocapsids to the sites of viral RNA synthesis, where VP30 is required to initiate primary viral transcription. We further demonstrate that a single serine residue at amino acid position 29 was sufficient to render VP30 active in primary transcription and to generate a recombinant virus with characteristics comparable to those of wild-type virus. In contrast, the rescue of a recombinant virus with a single serine at position 30 in VP30 was unsuccessful. Our results indicate critical roles for phosphorylated and dephosphorylated VP30 during the viral life cycle.
  
  
  
  The current Ebola virus outbreak in West Africa has caused more than 28,000 cases and 11,000 fatalities. Very little is known regarding the molecular mechanisms of how the Ebola virus transcribes and replicates its genome. Previous investigations showed that the transcriptional support activity of VP30 is activated upon VP30 dephosphorylation. The current study reveals that the situation is more complex and that primary transcription as well as the rescue of recombinant Ebola virus also requires the transient phosphorylation of VP30. VP30 encodes six N-proximal serine residues that serve as phosphorylation acceptor sites. The present study shows that the dynamic phosphorylation of serine at position 29 alone is sufficient to activate primary viral transcription. Our results indicate a series of phosphorylation/dephosphorylation events that trigger binding to and release from the nucleocapsid and transcription complex to be essential for the full activity of VP30.
authors.affiliation: Institute of Virology, Philipps University Marburg, Marburg, Germany.|German Center of Infection Research (DZIF), partner site Giessen-Marburg-Langen, Marburg, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Biedenkopf
authors.affiliation: Institute of Virology, Philipps University Marburg, Marburg, Germany.|German Center of Infection Research (DZIF), partner site Giessen-Marburg-Langen, Marburg, Germany.
authors.forename: Clemens
authors.initials: C
authors.lastname: Lier
authors.affiliation: Institute of Virology, Philipps University Marburg, Marburg, Germany becker@staff.uni-marburg.de.|German Center of Infection Research (DZIF), partner site Giessen-Marburg-Langen, Marburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Becker
mesh_terms: D000818:Animals; D029043:Ebolavirus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D057809:HEK293 Cells; D019142:Hemorrhagic Fever, Ebola / Q000821:virology; D006801:Humans; D019251:Nucleocapsid / Q000378:metabolism; D010766:Phosphorylation; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014157:Transcription Factors; C479897:VP30 protein, ebola virus; D014764:Viral Proteins