title: Immobilization of pectinase on silica-based supports: Impacts of particle size and spacer arm on the activity.
abstract: The pectinase was separately immobilized onto Florisil and nano silica supports through both glutaraldehyde and 3-glyoxypropyltrietoxysilane spacer arms. The effects of spacer arm, particle size of support and ionic liquids on the activities of pectinase preparations were investigated. The immobilization of pectinase onto Florisil and nano silica through 3-glyoxypropyltrietoxysilane spacer arm completely led to inactivation of enzyme; however, 10 and 75% pectinase activity were retained when it was immobilized through glutaraldehyde spacer arm onto Florisil and nano silica, respectively. The pectinase immobilized onto nano silica through glutaraldehyde spacer arm showed 6.3-fold higher catalytic efficiency than that of the pectinase immobilized onto Florisil through same spacer arm. A 2.3-fold increase in thermal stability of pectinase was provided upon immobilization onto nano silica at 35°C. The effects of IL/buffer mixture and volume ratio of IL/buffer mixture on the catalytic activities of free and immobilized pectinase preparations were also tested. All the pectinase preparations showed highest activity in 10% (v/v) 1-butyl-3-methylimidazolium hexafluorophosphate containing medium and their activities significantly affected from the concentration of 1-butyl-3-methylimidazolium hexafluorophosphate.
authors.affiliation: University of Cukurova, Vocational School of Imamoglu, Adana, Turkey. Electronic address: dalagoz@cu.edu.tr.
authors.forename: Dilek
authors.initials: D
authors.lastname: Alagöz
authors.affiliation: University of Cukurova, Faculty of Science and Letters, Department of Chemistry, 01330 Adana, Turkey.
authors.forename: S Seyhan
authors.initials: SS
authors.lastname: Tükel
authors.affiliation: University of Cukurova, Vocational School of Ceyhan, Adana, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Yildirim
mesh_terms: D001230:Aspergillus / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry; D010316:Particle Size; D011096:Polygalacturonase / Q000737:chemistry* / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D052578:Ionic Liquids; D012822:Silicon Dioxide; D011096:Polygalacturonase
keywords: Florisil; Nano silica; Pectinase