title: ZnO nanopellets have selective anticancer activity.
abstract: This research work presents the synthesis of ZnO nanopellets (ZNPs) by low temperature hydrothermal approach and evaluation of their antibacterial activity, cytotoxicity in vitro and in vivo. Structural and morphological studies conducted on the sample reveal hexagonal ZNPs in the size range of 250-500 nm. Surface area measurements showed high porosity of the sample compared to conventional ZnO nanoparticles. Antimicrobial studies revealed their bactericidal nature against both Gram-negative and Gram-positive bacteria. Furthermore, to better understand the parameters that affect the interactions between our ZNPs and mammalian cells, and thus their biocompatibility, we have examined the impact of cell culture conditions as well as of material properties on cytotoxicity by DPPH, blood hemolysis and MTT assay. The results showed good antioxidant capacity and biocompatibility of ZNPs at higher concentrations. MTT assay revealed the anticancer activity of ZNPs against prostate and breast cancer cell lines. Acute toxicity tests on Swiss albino mice showed no evident toxicity over a 14 days period.
authors.affiliation: Department of Chemistry, Sir M. Visvesvaraya Institute of Technology, Bengaluru 562157, India; Research and Development Centre, Bharathiar University, Coimbatore 641046, India.
authors.forename: Prashanth
authors.initials: P
authors.lastname: Gopala Krishna
authors.affiliation: Research and Development Centre, Bharathiar University, Coimbatore 641046, India; Department of Chemistry, Sai Vidya Institute of Technology, Bengaluru 560064, India. Electronic address: prsnthmysore@gmail.com.
authors.forename: Prashanth
authors.initials: P
authors.lastname: Paduvarahalli Ananthaswamy
authors.affiliation: Nanotechnology Research Centre, SRM University, Chennai 603203, India.
authors.forename: Tejabhiram
authors.initials: T
authors.lastname: Yadavalli
authors.affiliation: Department of Chemistry, M. S. Ramaiah Institute of Technology, Bengaluru 560054, India.
authors.forename: Nagabhushana
authors.initials: N
authors.lastname: Bhangi Mutta
authors.affiliation: Department of Chemistry, University of Mysore, Mysuru 560006, India.
authors.forename: Ananda
authors.initials: A
authors.lastname: Sannaiah
authors.affiliation: Drug Discovery Research and Development Centre, Skanda Life Sciences Private Limited, Bengaluru 560091, India.
authors.forename: Yogisha
authors.initials: Y
authors.lastname: Shivanna
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D010316:Particle Size; D012756:Sheep; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023382:Toxicity Tests, Acute; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D016166:Free Radical Scavengers; D015034:Zinc Oxide
keywords: Antibacterial; DPPH assay; Hemocompatibility; In vivo acute toxicity; MTT assay; ZnO nanopellets