title: High incidence of TERT mutation in brain tumor cell lines.
abstract: TERT promoter gene mutations are highly recurrent in malignant glioma. However, little information exists regarding their presence in experimental brain tumor models. To better characterize systems in which TERT mutation studies could be appropriately modeled experimentally, the TERT promoter was examined by conventional sequencing in primary brain tumor initiating cells (BTIC), two matched recurrent BTIC lines, a panel of established malignant glioma cell lines, and two meningioma cell lines. Telomerase gene expression was examined by quantitative PCR. We found that all glioblastoma BTIC lines harbored a TERT mutation, which was retained in two patient-matched recurrent BTIC. The TERT C228T or C250T mutation was found in 33/35 (94 %) of established malignant glioma cell lines and both meningioma cell lines examined. Brain tumor cell lines expressed variably high telomerase levels. Thus, a high percentage of glioma cell lines, as well as two meningioma cell lines, harbors TERT mutations. These data characterize tractable, accessible models with which to further explore telomerase biology in these tumor types.
authors.affiliation: Division of Oncology, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.|Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Tanner M
authors.initials: TM
authors.lastname: Johanns
authors.affiliation: Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, USA.|Department of Neurological Surgery, Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, 660 South Euclid, Box 8057, St. Louis, MO, 63110, USA.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, USA.|Department of Neurological Surgery, Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, 660 South Euclid, Box 8057, St. Louis, MO, 63110, USA.
authors.forename: Dale K
authors.initials: DK
authors.lastname: Kobayashi
authors.affiliation: Department of Neurosurgery, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mei
authors.affiliation: Department of Neurosurgery, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: Ian F
authors.initials: IF
authors.lastname: Dunn
authors.affiliation: Department of Neurological Surgery, Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, 660 South Euclid, Box 8057, St. Louis, MO, 63110, USA.
authors.forename: Diane D
authors.initials: DD
authors.lastname: Mao
authors.affiliation: Department of Neurological Surgery, Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, 660 South Euclid, Box 8057, St. Louis, MO, 63110, USA.|The Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Albert H
authors.initials: AH
authors.lastname: Kim
authors.affiliation: Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, USA. gpdunn@wustl.edu.|Department of Neurological Surgery, Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, 660 South Euclid, Box 8057, St. Louis, MO, 63110, USA. gpdunn@wustl.edu.|The Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine, St. Louis, MO, USA. gpdunn@wustl.edu.
authors.forename: Gavin P
authors.identifier: http://orcid.org/0000-0001-9302-4834
authors.initials: GP
authors.lastname: Dunn
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D015870:Gene Expression; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics; D008579:Meningioma / Q000235:genetics; D009154:Mutation; D009364:Neoplasm Recurrence, Local; D016133:Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism
chemical_list: C509186:TERT protein, human; D019098:Telomerase
keywords: Brain tumor initiating cells; Cancer; Glioblastoma; Glioma; Meningioma; TERT; Telomerase