title: Deleterious effects of tributyltin on porcine vascular stem cells physiology.
abstract: The vascular functional and structural integrity is essential for the maintenance of the whole organism and it has been demonstrated that different types of vascular progenitor cells resident in the vessel wall play an important role in this process. The purpose of the present research was to observe the effect of tributyltin (TBT), a risk factor for vascular disorders, on porcine Aortic Vascular Precursor Cells (pAVPCs) in term of cytotoxicity, gene expression profile, functionality and differentiation potential. We have demonstrated that pAVPCs morphology deeply changed following TBT treatment. After 48h a cytotoxic effect has been detected and Annexin binding assay demonstrated that TBT induced apoptosis. The transcriptional profile of characteristic pericyte markers has been altered: TBT 10nM substantially induced alpha-SMA, while, TBT 500nM determined a significant reduction of all pericyte markers. IL-6 protein detected in the medium of pAVPCs treated with TBT at both doses studied and with a dose response. TBT has interfered with normal pAVPC functionality preventing their ability to support a capillary-like network. In addition TBT has determined an increase of pAVPC adipogenic differentiation. In conclusion in the present paper we have demonstrated that TBT alters the vascular stem cells in terms of structure, functionality and differentiating capability, therefore effects of TBT in blood should be deeply explored to understand the potential vascular risk associated with the alteration of vascular stem cell physiology.
authors.affiliation: Department of Veterinary Medical Sciences - DIMEVET, University of Bologna, Ozzano Emilia, Bologna, Italy. Electronic address: chiara.bernardini5@unibo.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Bernardini
authors.affiliation: Department of Veterinary Medical Sciences - DIMEVET, University of Bologna, Ozzano Emilia, Bologna, Italy.
authors.forename: Augusta
authors.initials: A
authors.lastname: Zannoni
authors.affiliation: Department of Veterinary Medical Sciences - DIMEVET, University of Bologna, Ozzano Emilia, Bologna, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Bertocchi
authors.affiliation: Stem Wave Institute for Tissue Healing (SWITH), Gruppo Villa Maria (GVM) Care & Research - Ettore Sansavini Health Science Foundation, Lugo, Ravenna, Italy; National Institute of Biostructures and Biosystems at the Department of Experimental, Diagnostic and Specialty Medicine, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Bianchi
authors.affiliation: Department of Veterinary Medical Sciences - DIMEVET, University of Bologna, Ozzano Emilia, Bologna, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Salaroli
authors.affiliation: Department of Veterinary Medical Sciences - DEVET, UNICENTRO - Universidade Estadual do Centro-Oeste do Paraná, Brazil.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Botelho
authors.affiliation: Department of Veterinary Medical Sciences - DIMEVET, University of Bologna, Ozzano Emilia, Bologna, Italy.
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Bacci
authors.affiliation: Department of Veterinary Medical Sciences - DIMEVET, University of Bologna, Ozzano Emilia, Bologna, Italy.
authors.forename: Vittoria
authors.initials: V
authors.lastname: Ventrella
authors.affiliation: Department of Veterinary Medical Sciences - DIMEVET, University of Bologna, Ozzano Emilia, Bologna, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Forni
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D066026:Endothelial Progenitor Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D015850:Interleukin-6 / Q000378:metabolism; D020286:Pericytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D013552:Swine; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D014220:Trialkyltin Compounds; C011559:tributyltin
keywords: Pericyte; Pig model; Stem cells; Tributyltin; Vascular disorders