title: Morphological changes in experimental tuberculosis resulting from treatment with quercetin and polyvinylpyrrolidone.
abstract: OBJECTIVE
  Morphological study of tissue necrosis stages in experimental organ-preserving tuberculosis pharmacotherapy using Quercetin and Polyvinylpyrrolidone (QP).
  
  
  METHODS
  32 laboratory mice of C57BL/6JLacSto strain were used in the experiment. The animals were divided into five groups, six to seven mice in each: group 1- Mycobacterium tuberculosis (MBT) uninfected mice; group 2- MBT infected mice; group 3- MBT infected and treated with antituberculosis preparation (ATP); group 4- MBT infected and QP treated; group 5- MBT infected and treated with ATP and QP. The mice were infected through caudal vein injection with MTB H37Rv strain. The preparation QP, which belongs to the capillary-stabilizing-remedy group, was used for the research. The ATP were izoniazid and streptomycin.
  
  
  RESULTS
  QP produced a strict delineation of caseous necrosis from the unaffected parts of the connective tissue with fibrosis in the center and a large number of Langerhans cells, which was not observed in the control groups without QP. The combination of QP and ATP had more pronounced effects. In MBT-infected mice, where QP was not used, unlike the group where QP was used, adipose dystrophy of hepatocytes was observed. Thus, the hepatoprotective effect of QP against TB can be suggested.
  
  
  CONCLUSIONS
  QP produces a clear delineation of caseous necrosis from an uninfected tissue by connective-tissue formation, and by forming fibrotic tissue in the center of epithelioid cells that prevents further TB dissemination by enhancing TB pharmacotherapy.
authors.affiliation: Department of of Phthisiology and Pulmonology, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine. Electronic address: dddimad@yandex.ua.
authors.forename: Dmytro O
authors.initials: DO
authors.lastname: Butov
authors.affiliation: Department of of Phthisiology and Pulmonology, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine.
authors.forename: Svetlana I
authors.initials: SI
authors.lastname: Zaitseva
authors.affiliation: Department of Pathomorphology, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine.
authors.forename: Mykola M
authors.initials: MM
authors.lastname: Pitenko
authors.affiliation: Department of of Phthisiology and Pulmonology, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine.
authors.forename: Ganna L
authors.initials: GL
authors.lastname: Stepanenko
authors.affiliation: Department of Internal Medicine N3, Kharkiv National Medical University, 4 Lenina Avenue, Kharkiv 61022, Ukraine.
authors.forename: Tatyana S
authors.initials: TS
authors.lastname: Butova
mesh_terms: D000825:Animal Structures / Q000187:drug effects / Q000473:pathology; D000818:Animals; D000995:Antitubercular Agents / Q000008:administration & dosage* / Q000009:adverse effects; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011205:Povidone / Q000008:administration & dosage* / Q000009:adverse effects; D011794:Quercetin / Q000008:administration & dosage* / Q000009:adverse effects; D014376:Tuberculosis / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000995:Antitubercular Agents; D011794:Quercetin; D011205:Povidone
keywords: Epithelioid cellular granuloma; Experimental model; Morphological changes; Polyvinylpyrrolidone; Quercetin; Treatment of tuberculosis