title: Inhibitory effect of Phalaenopsis orchid extract on WNT1-induced immature melanocyte precursor differentiation in a novel in vitro solar lentigo model.
abstract: Recently, it has been reported that increased expression of WNT1 accelerates the differentiation of melanocyte stem cells (McSCs) in solar lentigines (SLs), hyperpigmented maculae commonly seen on sun-exposed areas of the skin. In this study, to establish an in vitro SL model, human epidermal squamous carcinoma cell line HSC-1, which expresses higher levels of WNT1 than normal human epidermal keratinocytes, was co-cultured with early passage normal human epidermal melanocytes (NHEMs) as an in vitro McSC model. As a result, mRNA expression levels of melanocyte differentiation-related genes MITF and TYR in NHEMs were significantly increased by co-culturing with HSC-1 cells. Furthermore, Phalaenopsis orchid extract (Phex) inhibited McSCs differentiation by suppressing WNT1 expression via down-regulation of DLX2, a transcriptional activator of WNT1, in HSC-1 cells. Therefore, our finding suggested that extracts such as Phex, which suppresses WNT1 expression, may be useful as a novel treatment of SLs.
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Yamada
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.|b Nagoya University-Menard Collaborative Research Chair , Nagoya University Graduate School of Medicine , Nagoya , Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Hasegawa
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kunita
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ohsumi
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sakaida
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.
authors.forename: Youichi
authors.initials: Y
authors.lastname: Yashiro
authors.affiliation: a Research Laboratories , Nippon Menard Cosmetic Co., Ltd. , Nagoya , Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakata
mesh_terms: D000954:Antigens, Surface / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D018920:Coculture Techniques; D005786:Gene Expression Regulation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D007911:Lentigo / Q000517:prevention & control; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008954:Models, Biological; D029595:Orchidaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction; D014466:Ultraviolet Rays / Q000009:adverse effects; D051155:Wnt1 Protein / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D010936:Plant Extracts; D020011:Protective Agents; C495247:WNT1 protein, human; D051155:Wnt1 Protein; C092261:antigen Dlx-2
keywords: differentiation; melanocyte stem cell; pigmentation; solar lentigo