title: Comparative nutritional compositions and proteomics analysis of transgenic Xa21 rice seeds compared to conventional rice.
abstract: Transgenic rice expressing the Xa21 gene have enhanced resistant to most devastating bacterial blight diseases caused by Xanthomonas oryzae pv. oryzae (Xoo). However, identification of unintended modifications, owing to the genetic modification, is an important aspect of transgenic crop safety assessment. In this study, the nutritional compositions of seeds from transgenic rice plants expressing the Xa21 gene were compared against non-transgenic rice seeds. In addition, to detect any changes in protein translation levels as a result of Xa21 gene expression, rice seed proteome analyses were also performed by two-dimensional gel electrophoresis. No significant differences were found in the nutritional compositions (proximate components, amino acids, minerals, vitamins and anti-nutrients) of the transgenic and non-transgenic rice seeds. Although gel electrophoresis identified 11 proteins that were differentially expressed between the transgenic and non-transgenic seed, only one of these (with a 20-fold up-regulation in the transgenic seed) shows nutrient reservoir activity. No new toxins or allergens were detected in the transgenic seeds.
authors.affiliation: Laboratory for Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Dipak
authors.initials: D
authors.lastname: Gayen
authors.affiliation: Laboratory for Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Paul
authors.affiliation: Laboratory for Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Sailendra Nath
authors.initials: SN
authors.lastname: Sarkar
authors.affiliation: Laboratory for Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India.
authors.forename: Swapan K
authors.initials: SK
authors.lastname: Datta
authors.affiliation: Laboratory for Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India. Electronic address: krbdatta@yahoo.com.
authors.forename: Karabi
authors.initials: K
authors.lastname: Datta
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D009753:Nutritive Value; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D040901:Proteomics / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014974:Xanthomonas / Q000254:growth & development
chemical_list: D010940:Plant Proteins; C097148:Xa21 protein, Oryza sativa; D017346:Protein Serine-Threonine Kinases
keywords: Oryza sativa; Proteomics; Substantial equivalence; Transgenic rice; Xa21