title: Evaluation of Plasma miR-21 and miR-152 as Diagnostic Biomarkers for Common Types of Human Cancers.
abstract: Stable blood based miRNA species have allowed for the differentiation of patients with various types of cancer. Therefore, specific blood-based miRNA might be considered as a methodology which could be informative of the presence of cancer potentially from multiple distinct organ sites. Recently, miR-21 has been identified as an "oncomir" in various tumors while miR-152 as a tumor suppressor. In this study, we investigated whether circulating miR-21 and miR-152 can be used for early detection of lung cancer (LuCa), colorectal carcinoma (CRC), breast cancer (BrCa) and prostate cancer (PCa), with distinguishing cancer from various benign lesions on these organ sites. We measured the two miRNA levels by using real-time RT-PCR in plasma samples from a total of 204 cancer patients, 159 various benign lesions, and 228 normal subjects. We observed significantly elevated expression of miR-21 and miR-152 in LuCa, CRC, and BrCa when compared with normal controls. We also found upregulation of plasma miR-21 and miR-152 levels in patients with benign lesions of lung and breast, as compared to normal controls, respectively. No significant expression variation of the two miRNAs was observed in PCa or prostatic benign lesions as compared to healthy controls. Receiver operating characteristic (ROC) analyses revealed that miR-21 and/or miR-152 can discriminate LuCa, CRC and BrCa from normal controls. Our results suggest that plasma miR-21 and miR-152 may serve as non-specific noninvasive biomarkers for early screening of LuCa, CRC, and BrCa, but not PCa.
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;
authors.forename: Hankui
authors.initials: H
authors.lastname: Chen
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;; 8. Shenzhen Baoan District Shajing Hospital, Shenzhen, Guangdong 518104, China.
authors.forename: Helu
authors.initials: H
authors.lastname: Liu
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;
authors.forename: Hanqing
authors.initials: H
authors.lastname: Zou
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;; 8. Shenzhen Baoan District Shajing Hospital, Shenzhen, Guangdong 518104, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Dou
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;
authors.forename: Shile
authors.initials: S
authors.lastname: Sheng
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;; 9. Department of Clinical Laboratory, The Fourth Hospital Affiliated to Guangxi Medical University, Liuzhou, Guangxi 545005, China.
authors.forename: Shengming
authors.initials: S
authors.lastname: Dai
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;
authors.forename: Junmei
authors.initials: J
authors.lastname: Ai
authors.affiliation: 2. Department of Pathology, Rush University Medical Center, Chicago, IL 60612;
authors.forename: Joshua
authors.initials: J
authors.lastname: Melson
authors.affiliation: 6. Division of Urology, Department of Surgery, University of Arizona Cancer Center, Tucson, AZ 85724;
authors.forename: Rick A
authors.initials: RA
authors.lastname: Kittles
authors.affiliation: 7. Department of Psychiatry, School of Medicine, the Johns Hopkins University, Baltimore, MD 21287.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Pirooznia
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;; 2. Department of Pathology, Rush University Medical Center, Chicago, IL 60612;; 3. Department of Cardiothoracic Surgery, Rush University Medical Center, Chicago, IL 60612;
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Liptay
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;; 2. Department of Pathology, Rush University Medical Center, Chicago, IL 60612;; 4. Department of Biochemistry, Rush University Medical Center, Chicago, IL 60612;
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Borgia
authors.affiliation: 1. Rush University Cancer Center, Chicago, IL 60612;; 2. Department of Pathology, Rush University Medical Center, Chicago, IL 60612;; 5. Department of Internal Medicine, Rush University Medical Center, Chicago, IL 60612;
authors.forename: Youping
authors.initials: Y
authors.lastname: Deng
keywords: cancer; miRNA