title: Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP.
abstract: A PCR-Restriction Fragment Length Polymorphism (RFLP) targeting the mannose phosphate isomerase gene was established to differentiate Leishmania species distributed near the Department of Huanuco, Peru. The technique was applied to 267 DNA samples extracted from Giemsa-stained smears of cutaneous lesions taken from patients suspected for cutaneous leishmaniasis in the area, and the present status of causative Leishmania species was identified. Of 114 PCR-amplified samples, 22, 19, 24 and 49 samples were identified to be infected by Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, and a hybrid of L. (V.) braziliensis/L. (V.) peruviana, respectively, and the validity of PCR-RFLP was confirmed by sequence analysis. Since PCR-RFLP is simple and rapid, the technique will be a useful tool for the epidemiological study of leishmaniasis.
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Department of Microbiology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Koarashi
authors.affiliation: Sección de Entomología, Instituto de Medicina Tropical "Daniel A. Carrión" y Departamento Académico de Microbiología Médica, Facultad de Medicina Humana, Universidad Nacional Mayor de San Marcos, Lima, Peru; Laboratorio de Entomología, Instituto Nacional de Salud, Lima, Peru.
authors.forename: Abraham G
authors.initials: AG
authors.lastname: Cáceres
authors.affiliation: Laboratorio de Referencia Regional de Huánuco, DIRESA Huánuco, Peru.
authors.forename: Florencia Margarita Zúniga
authors.initials: FMZ
authors.lastname: Saca
authors.affiliation: Dirección Ejecutiva de Epidemiologia, DIRESA Huánuco, Peru.
authors.forename: Elsa Elvira Palacios
authors.initials: EEP
authors.lastname: Flores
authors.affiliation: Dirección Ejecutiva de Epidemiologia, DIRESA Huánuco, Peru.
authors.forename: Adela Celis
authors.initials: AC
authors.lastname: Trujillo
authors.affiliation: Vigilancia Entomológica y Control Vectorial, Dirección de Saneamiento Ambiental, DIRESA Huánuco, Peru.
authors.forename: José Luis Abanto
authors.initials: JLA
authors.lastname: Alvares
authors.affiliation: Department of Microbiology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.affiliation: Department of Microbiology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Katakura
authors.affiliation: Parasitologia y Medicina Tropical, Facultad de Medicina, Universidad Catolica de Santiago de Guayaquil, Guayaquil, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hashiguchi
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. Electronic address: hkato@vetmed.hokudai.ac.jp.
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Kato
mesh_terms: D001399:Azure Stains; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics*; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology* / Q000235:genetics*; D010568:Peru / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D001399:Azure Stains
keywords: Leishmania; Mannose phosphate isomerase; PCR-RFLP; Peru