title: Simultaneous determination of mangiferin and neomangiferin in rat plasma by UPLC-MS/MS and its application for pharmacokinetic study.
abstract: In this study, a sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine mangiferin and neomangiferin in rat plasma simultaneously. Chromatographic separation was carried out on an Acquity UPLC BEH C18 column and mass spectrometric analysis was performed using a Xevo TQD triple quadruple mass spectrometer coupled with an electrospray ionization (ESI) source. The MRM transitions of m/z 423.2 → 303.1 and m/z 585.0 → 273.1 were used to quantify for mangiferin and neomangiferin, respectively. The linearity of this method was found to be within the concentration range of 5-2000 ng/mL for mangiferin, and 2-1000 ng/mL for neomangiferin in rat plasma, respectively. Only 3.0 min was needed for an analytical run. This assay was used to support a preclinical study to investigate the pharmacokinetics of mangiferin and neomangiferin in rats.
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Jian-Long
authors.initials: JL
authors.lastname: Zhao
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Hao
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Canli
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Medical College of Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Nuan
authors.initials: N
authors.lastname: Tian
authors.affiliation: The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: xuzhisheng1111@163.com.
authors.forename: Zhi-Sheng
authors.initials: ZS
authors.lastname: Xu
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: ruanminzou@163.com.
authors.forename: Ruan-Min
authors.initials: RM
authors.lastname: Zou
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005960:Glucosides / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*; D044004:Xanthones / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D005960:Glucosides; D044004:Xanthones; C442164:neomangiferin; C013592:mangiferin
keywords: Mangiferin; Neomangiferin; Rat plasma; UPLC–MS/MS