title: Sublethal effects of chlorantraniliprole on development, reproduction and vitellogenin gene (CsVg) expression in the rice stem borer, Chilo suppressalis.
abstract: BACKGROUND
  The rice stem borer, Chilo suppressalis (Walker), is one of the most damaging rice pests in the world. The sublethal effects of chlorantraniliprole on development,reproduction and mRNA expression levels of vitellogenin gene (CsVg) in C. suppressalis were investigated.
  
  
  RESULTS
  Exposure of third-instar larvae to sublethal concentrations of chlorantraniliprole (LC10and LC30) significantly extended larval duration, lowered the mean weight of male pupae and shortened male adult longevity. Pupal duration was significantly prolonged and the mean weight of female pupae was significantly lowered in the LC30treatment group. While there were no significant sublethal effects on either the adult emergence rate or the egg hatch, the pupation rates in the LC10treatment group (41.30%) and in the LC30treatment group (23.98%) were significantly lower than the pupation rate of the control (71.86%), and LC10and LC30chlorantraniliprole significantly reduced fecundity, by 32.18 and 52.94% respectively. Furthermore, the expression levels of CsVg mRNA after exposure to LC10and LC30chlorantraniliprole significantly decreased, by 42.52 and 47.84% respectively, in 12-h-old female adults.
  
  
  CONCLUSIONS
  Sublethal concentrations of chlorantraniliprole adversely affect the development and reproduction of C. suppressalis. The downregulation of CsVg by chlorantraniliprole might have negative impacts on the fecundity of C. suppressalis. © 2016 Society of Chemical Industry.
authors.affiliation: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Lu
authors.affiliation: Jiangsu Lixiahe Institute of Agricultural Sciences, Yangzhou, China.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Han
authors.affiliation: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China.
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China. wangjj@yzu.edu.cn.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D005298:Fertility / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000235:genetics; D007306:Insecticides / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009036:Moths / Q000187:drug effects* / Q000502:physiology; D011679:Pupa / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects; D012744:Sex Ratio; D014819:Vitellogenins / Q000235:genetics*; D062367:ortho-Aminobenzoates / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D014819:Vitellogenins; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole
keywords: Chilo suppressalis; chlorantraniliprole; sublethal effects; vitellogenin gene