title: Effects of Efflux Pump Inhibitors on Colistin Resistance in Multidrug-Resistant Gram-Negative Bacteria.
abstract: We tested the effects of various putative efflux pump inhibitors on colistin resistance in multidrug-resistant Gram-negative bacteria. Addition of 10 mg/liter cyanide 3-chlorophenylhydrazone (CCCP) to the test medium could significantly decrease the MICs of colistin-resistant strains. Time-kill assays showed CCCP could reverse colistin resistance and inhibit the regrowth of the resistant subpopulation, especially in Acinetobacter baumannii and Stenotrophomonas maltophilia These results suggest colistin resistance in Gram-negative bacteria can be suppressed and reversed by CCCP.
authors.affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Ni
authors.affiliation: Department of Clinical Laboratory, Navy General Hospital, Beijing, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Peking University First Hospital, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Guan
authors.affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, China guoguoyoumeng@163.com liuyouningpla301@163.com.
authors.forename: Junchang
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, China guoguoyoumeng@163.com liuyouningpla301@163.com.
authors.forename: Youning
authors.initials: Y
authors.lastname: Liu
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003091:Colistin / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D020615:Stenotrophomonas maltophilia / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D003091:Colistin