title: Improvement of exopolysaccharide production by Porphyridium marinum.
abstract: With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005. Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure. In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production. A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière CEDEX, France.
authors.forename: Nastasia
authors.initials: N
authors.lastname: Soanen
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière CEDEX, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Da Silva
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière CEDEX, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Gardarin
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière CEDEX, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Michaud
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière CEDEX, France. Electronic address: celine.laroche@univ-bpclermont.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Laroche
mesh_terms: D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058433:Photobioreactors; D010788:Photosynthesis; D011134:Polysaccharides / Q000096:biosynthesis*; D044665:Porphyridium / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D011134:Polysaccharides
keywords: Microalgae; Photobioreactor; Polysaccharide; Porphyridium; Semi-continuous culture