title: Effects of Disinfectants on Larval Development of Ascaris suum Eggs.
abstract: The objective of this study was to evaluate the effects of several different commercial disinfectants on the embryogenic development of Ascaris suum eggs. A 1-ml aliquot of each disinfectant was mixed with approximately 40,000 decorticated or intact A. suum eggs in sterile tubes. After each treatment time (at 0.5, 1, 5, 10, 30, and 60 min), disinfectants were washed away, and egg suspensions were incubated at 25˚C in distilled water for development of larvae inside. At 3 weeks of incubation after exposure, ethanol, methanol, and chlorohexidin treatments did not affect the larval development of A. suum eggs, regardless of their concentration and treatment time. Among disinfectants tested in this study, 3% cresol, 0.2% sodium hypochlorite and 0.02% sodium hypochlorite delayed but not inactivated the embryonation of decorticated eggs at 3 weeks of incubation, because at 6 weeks of incubation, undeveloped eggs completed embryonation regardless of exposure time, except for 10% povidone iodine. When the albumin layer of A. suum eggs remained intact, however, even the 10% povidone iodine solution took at least 5 min to reasonably inactivate most eggs, but never completely kill them with even 60 min of exposure. This study demonstrated that the treatment of A. suum eggs with many commercially available disinfectants does not affect the embryonation. Although some disinfectants may delay or stop the embryonation of A. suum eggs, they can hardly kill them completely.
authors.affiliation: Department of Obstetrics, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Ki-Seok
authors.initials: KS
authors.lastname: Oh
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Geon-Tae
authors.initials: GT
authors.lastname: Kim
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Kyu-Sung
authors.initials: KS
authors.lastname: Ahn
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Sung-Shik
authors.initials: SS
authors.lastname: Shin
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000187:drug effects*; D004202:Disinfectants / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D013997:Time Factors
chemical_list: D004202:Disinfectants
keywords: Ascaris suum; disinfectant; egg; larval development