title: Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice.
abstract: Clausena anisata (Willd.) Hook.f. ex Benth. (CA), which is widely used in traditional medicine, reportedly exerts antitumor, anti-inflammatory and other important therapeutic effects. The aim of the present study was to investigate the potential therapeutic effects of CA in a mouse model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and in LPS-stimulated RAW 264.7 cells. Male C57BL/6 mice were administered treatments for 3 days by oral gavage. On day 3, the mice were instilled intranasally with LPS or PBS followed 3 h later by oral CA (30 mg/kg) or vehicle administration. In vitro, CA decreased nitric oxide (NO) production and pro-inflammatory cytokines, such as interleukin (IL)-6 and prostaglandin E2 (PGE2), in LPS-stimulated RAW 264.7 cells. CA also reduced the expression of pro-inflammatory mediators, such as cyclooxygenase-2. In vivo, CA administration significantly reduced inflammatory cell numbers in the bronchoalveolar lavage fluid (BALF) and suppressed pro-inflammatory cytokine levels, including tumor necrosis factor-α (TNF-α), IL-6, and IL-1β, as well as reactive oxygen species production in the BALF. CA also effectively reduced airway inflammation in mouse lung tissue of an LPS-induced ALI mouse model, in addition to decreasing inhibitor κB (IκB) and nuclear factor-κB (NF-κB) p65 phosphorylation. Taken together, the findings demonstrated that CA inhibited inflammatory responses in a mouse model of LPS-induced ALI and in LPS-stimulated RAW 264.7 cells. Thus, CA is a potential candidate for development as an adjunctive treatment for inflammatory disorders, such as ALI.
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: Chan-Mi
authors.initials: CM
authors.lastname: Jeon
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: In-Sik
authors.initials: IS
authors.lastname: Shin
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: Na-Rae
authors.initials: NR
authors.lastname: Shin
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: Ju-Mi
authors.initials: JM
authors.lastname: Hong
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: Ok-Kyoung
authors.initials: OK
authors.lastname: Kwon
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.affiliation: IEBR, Vietnam Academy of Science and Technology (VAST), Cay Giay, Ha Noi, Vietnam.
authors.forename: Tran-The
authors.initials: TT
authors.lastname: Bach
authors.affiliation: IEBR, Vietnam Academy of Science and Technology (VAST), Cay Giay, Ha Noi, Vietnam.
authors.forename: Do-Van
authors.initials: DV
authors.lastname: Hai
authors.affiliation: IEBR, Vietnam Academy of Science and Technology (VAST), Cay Giay, Ha Noi, Vietnam.
authors.forename: Bui-Hong
authors.initials: BH
authors.lastname: Quang
authors.affiliation: International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong‑gu, Daejeon 34141, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Choi
authors.affiliation: International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong‑gu, Daejeon 34141, Republic of Korea.
authors.forename: Joongku
authors.initials: J
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungnam National University, Yuseong‑gu, Daejeon, Chungcheongnam‑do 34134, Republic of Korea.
authors.forename: Pyung-Keun
authors.initials: PK
authors.lastname: Myung
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea.
authors.forename: Kyung-Seop
authors.initials: KS
authors.lastname: Ahn
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use*; D054700:Clausena / Q000737:chemistry*; D016207:Cytokines / Q000276:immunology; D015232:Dinoprostone / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone