title: A direct role for murine Cdx proteins in the trunk neural crest gene regulatory network.
abstract: Numerous studies in chordates and arthropods currently indicate that Cdx proteins have a major ancestral role in the organization of post-head tissues. In urochordate embryos, Cdx loss-of-function has been shown to impair axial elongation, neural tube (NT) closure and pigment cell development. Intriguingly, in contrast to axial elongation and NT closure, a Cdx role in neural crest (NC)-derived melanocyte/pigment cell development has not been reported in any other chordate species. To address this, we generated a new conditional pan-Cdx functional knockdown mouse model that circumvents Cdx functional redundancy as well as the early embryonic lethality of Cdx mutants. Through directed inhibition in the neuroectoderm, we provide in vivo evidence that murine Cdx proteins impact melanocyte and enteric nervous system development by, at least in part, directly controlling the expression of the key early regulators of NC ontogenesis Pax3,Msx1 and Foxd3 Our work thus reveals a novel role for Cdx proteins at the top of the trunk NC gene regulatory network in the mouse, which appears to have been inherited from their ancestral ortholog.
authors.affiliation: Molecular Genetics of Development Laboratory, Department of Biological Sciences and BioMed Research Center, University of Quebec at Montreal (UQAM), Montreal H2X 3Y7, Canada.
authors.forename: Oraly
authors.initials: O
authors.lastname: Sanchez-Ferras
authors.affiliation: Molecular Genetics of Development Laboratory, Department of Biological Sciences and BioMed Research Center, University of Quebec at Montreal (UQAM), Montreal H2X 3Y7, Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bernas
authors.affiliation: Molecular Genetics of Development Laboratory, Department of Biological Sciences and BioMed Research Center, University of Quebec at Montreal (UQAM), Montreal H2X 3Y7, Canada.
authors.forename: Omar
authors.initials: O
authors.lastname: Farnos
authors.affiliation: Molecular Genetics of Development Laboratory, Department of Biological Sciences and BioMed Research Center, University of Quebec at Montreal (UQAM), Montreal H2X 3Y7, Canada.
authors.forename: Aboubacrine M
authors.initials: AM
authors.lastname: Touré
authors.affiliation: Molecular Genetics of Development Laboratory, Department of Biological Sciences and BioMed Research Center, University of Quebec at Montreal (UQAM), Montreal H2X 3Y7, Canada.
authors.forename: Ouliana
authors.initials: O
authors.lastname: Souchkova
authors.affiliation: Molecular Genetics of Development Laboratory, Department of Biological Sciences and BioMed Research Center, University of Quebec at Montreal (UQAM), Montreal H2X 3Y7, Canada pilon.nicolas@uqam.ca.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pilon
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D051858:Forkhead Transcription Factors / Q000235:genetics; D055785:Gene Knockdown Techniques; D053263:Gene Regulatory Networks; D018398:Homeodomain Proteins / Q000378:metabolism; D051957:MSX1 Transcription Factor / Q000235:genetics; D008544:Melanocytes / Q000166:cytology; D051379:Mice; D008954:Models, Biological; D009432:Neural Crest / Q000166:cytology*; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics
chemical_list: C491272:Cdx1 protein, mouse; D051858:Forkhead Transcription Factors; C101757:Foxd3 protein, mouse; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; C484084:Msx1 protein, mouse; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; C068686:Pax3 protein, mouse
keywords: Cdx; Enteric nervous system; Gene regulatory network; Melanocyte; Neural crest; Peripheral nervous system