title: Quantitative Detection of Nucleoside Analogues by Multi-enzyme Biosensors using Time-Resolved Kinetic Measurements.
abstract: Fast, simple and cost-effective methods for detecting and quantifying pharmaceutical agents in patients are highly sought after to replace equipment and labor-intensive analytical procedures. The development of new diagnostic technology including portable detection devices also enables point-of-care by non-specialists in resource-limited environments. We have focused on the detection and dose monitoring of nucleoside analogues used in viral and cancer therapies. Using deoxyribonucleoside kinases (dNKs) as biosensors, our chemometric model compares observed time-resolved kinetics of unknown analytes to known substrate interactions across multiple enzymes. The resulting dataset can simultaneously identify and quantify multiple nucleosides and nucleoside analogues in complex sample mixtures.
authors.affiliation: Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA, 30322, USA.
authors.forename: Pravin
authors.initials: P
authors.lastname: Muthu
authors.affiliation: Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA, 30322, USA. sal2@emory.edu.
authors.forename: Stefan
authors.identifier: http://orcid.org/0000-0003-1043-0162
authors.initials: S
authors.lastname: Lutz
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004331:Drosophila melanogaster / Q000201:enzymology; D007700:Kinetics; D009705:Nucleosides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009705:Nucleosides; D017853:Phosphotransferases (Alcohol Group Acceptor); C020018:deoxyribonucleoside kinases
keywords: biosensors; data fusion; deoxynucleoside kinases; nucleoside analogues; therapeutic dosage monitoring