title: Changes of diet and dominant intestinal microbes in farmland frogs.
abstract: BACKGROUND
  Agricultural activities inevitably result in anthropogenic interference with natural habitats. The diet and the gut microbiota of farmland wildlife can be altered due to the changes in food webs within agricultural ecosystems. In this work, we compared the diet and intestinal microbiota of the frog Fejervarya limnocharis in natural and farmland habitats in order to understand how custom farming affects the health of in vivo microbial ecosystems.
  
  
  RESULTS
  The occurrence, abundance, and the numbers of prey categories of stomach content were significantly different between the frogs inhabiting natural and farmland habitats. In addition, differences in the abundance, species richness, and alpha-diversity of intestinal microbial communities were also statistically significant. The microbial composition, and particularly the composition of dominant microbes living in intestines, indicated that the land use practices might be one of factors affecting the gut microbial community composition. Although the first three dominant microbial phyla Bacteroidetes, Firmicutes, and Proteobacteria found in the intestines of frogs were classified as generalists among habitats, the most dominant gut bacterial phylum Bacteroidetes in natural environments was replaced by the microbial phylum Firmicutes in farmland frogs. Increased intestinal microbial richness of the farmland frogs, which is mostly contributed by numerous microbial species of Proteobacteria, Actinobacteria, Acidobacteria, and Planctomycetes, not only reflects the possible shifts in microbial community composition through the alteration of external ecosystem, but also indicates the higher risk of invasion by disease-related microbes.
  
  
  CONCLUSIONS
  This study indicates that anthropogenic activities, such as the custom farming, have not only affected the food resources of frogs, but also influenced the health and in vivo microbial ecosystem of wildlife.
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, 11677, Taiwan.|Taiwan Forestry Research Institute, Technical Service Division, Taipei, 10066, Taiwan.
authors.forename: Chun-Wen
authors.initials: CW
authors.lastname: Chang
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, 11677, Taiwan.
authors.forename: Bing-Hong
authors.initials: BH
authors.lastname: Huang
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, 11677, Taiwan.
authors.forename: Si-Min
authors.initials: SM
authors.lastname: Lin
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, 11677, Taiwan.
authors.forename: Chia-Lung
authors.initials: CL
authors.lastname: Huang
authors.affiliation: Department of Life Science, National Taiwan Normal University, Taipei, 11677, Taiwan. pcliao@ntnu.edu.tw.
authors.forename: Pei-Chun
authors.initials: PC
authors.lastname: Liao
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001001:Anura / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017753:Ecosystem; D005260:Female; D000069196:Gastrointestinal Microbiome; D007422:Intestines / Q000382:microbiology*; D008297:Male; D010802:Phylogeny
keywords: Abundance; Custom farming; Diet; Fejervarya limnocharis; Gut microbiota; Richness