title: Synergistic combination of antioxidants, silver nanoparticles and chitosan in a nanoparticle based formulation: Characterization and cytotoxic effect on MCF-7 breast cancer cell lines.
abstract: OBJECTIVE
  Chitosan (Cs) is a biocompatible, biodegradable cationic polymer having the ability of targeted drug delivery. Vitamin E and C are not synthesized in our body thus, when encapsulated within a carrier system these vitamins in combination with/alone can be utilized for their anti-cancer potentials.
  
  
  METHODS
  The present investigation was conducted to develop a stable nanoparticle based formulation encapsulating antioxidants (Vitamin E, catechol) and silver nanoparticles synthesized from Hibiscus rosa-sinensis (HRS) petal extracts within a chitosan matrix. The prepared nanoformulations were characterized using Field emission scanning electron microscopy (Fe-SEM), X-ray diffraction (XRD) and Attenuated Total Reflection Fourier Transform Infrared spectroscopy (ATR-FTIR). They were further tested for their antioxidant potentials using DPPH assay, hydrogen peroxide scavenging assay, nitric oxide scavenging assay and ferrous antioxidant reducing potential assay.
  
  
  RESULTS
  The nanoformulations were found to be highly hemocompatible and showed high encapsulation efficiency up to 76%. They also showed higher antioxidant activity than their base materials. Further, their anti-cancer efficacy was observed against MCF-7 breast cancer cells having IC50 values of 53.36±0.36μg/mL (chitosan-ascorbic acid-glucose), 55.28±0.85μg/mL (chitosan-Vitamin E), 63.72±0.27μg/mL (Chitosan-catechol) and 58.53±0.55μg/mL (chitosan-silver nanoparticles). Thus, the prepared formulations can be therapeutically applied for effective and targeted delivery in breast cancer treatment.
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Debasis
authors.initials: D
authors.lastname: Nayak
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Aliva Prity
authors.initials: AP
authors.lastname: Minz
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Sarbani
authors.initials: S
authors.lastname: Ashe
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Pradipta Ranjan
authors.initials: PR
authors.lastname: Rauta
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Kumari
authors.affiliation: Department of Biotechnology, Thapar University, Patiala, Punjab, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Chopra
authors.affiliation: Immunology and Molecular Medicine Laboratory, Department of Life Science, National Institute of Technology, Rourkela, Odisha 769008, India. Electronic address: nayakb@nitrkl.a.cin.
authors.forename: Bismita
authors.initials: B
authors.lastname: Nayak
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D031584:Hibiscus / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D012834:Silver; D048271:Chitosan
keywords: Anti-cancer activity; Antioxidants; Chitosan; Hemocompatible; Nanoparticles; Silver nanoparticles; Targeted drug delivery; Vitamin C; Vitamin E