title: Construction of High-Density Linkage Maps of Populus deltoides × P. simonii Using Restriction-Site Associated DNA Sequencing.
abstract: Although numerous linkage maps have been constructed in the genus Populus, they are typically sparse and thus have limited applications due to low throughput of traditional molecular markers. Restriction-site associated DNA sequencing (RADSeq) technology allows us to identify a large number of single nucleotide polymorphisms (SNP) across genomes of many individuals in a fast and cost-effective way, and makes it possible to construct high-density genetic linkage maps. We performed RADSeq for 299 progeny and their two parents in an F1 hybrid population generated by crossing the female Populus deltoides 'I-69' and male Populus simonii 'L3'. A total of 2,545 high quality SNP markers were obtained and two parent-specific linkage maps were constructed. The female genetic map contained 1601 SNPs and 20 linkage groups, spanning 4,249.12 cM of the genome with an average distance of 2.69 cM between adjacent markers, while the male map consisted of 940 SNPs and also 20 linkage groups with a total length of 3,816.24 cM and an average marker interval distance of 4.15 cM. Finally, our analysis revealed that synteny and collinearity are highly conserved between the parental linkage maps and the reference genome of P. trichocarpa. We demonstrated that RAD sequencing is a powerful technique capable of rapidly generating a large number of SNPs for constructing genetic maps in outbred forest trees. The high-quality linkage maps constructed here provided reliable genetic resources to facilitate locating quantitative trait loci (QTLs) that control growth and wood quality traits in the hybrid population.
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Chunfa
authors.initials: C
authors.lastname: Tong
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Huogen
authors.initials: H
authors.lastname: Li
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Xuran
authors.initials: X
authors.lastname: Li
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Ou
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Deyuan
authors.initials: D
authors.lastname: Wang
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Houxi
authors.initials: H
authors.lastname: Xu
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Xianye
authors.initials: X
authors.lastname: Lang
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Liu
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: The Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China.
authors.forename: Jisen
authors.initials: J
authors.lastname: Shi
mesh_terms: D002678:Chimera / Q000235:genetics*; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D008040:Genetic Linkage; D018745:Genome, Plant; D059014:High-Throughput Nucleotide Sequencing; D020641:Polymorphism, Single Nucleotide; D032107:Populus / Q000235:genetics*
chemical_list: D018744:DNA, Plant