title: Studies of magnetic alginate-based electrospun matrices crosslinked with different methods for potential hyperthermia treatment.
abstract: The magnetic electrospun mats were lately established as an innovative biomaterial for hyperthermic cancer treatment. Unlike those surface-modified magnetic nanoparticles that may not firmly adhere onto the tumor for long-term duration, the magnetic mats with nanofibrous structure can promote cell adhesion and kill the tumor directly within an alternating magnetic field. However, most magnetic electrospun mats were fabricated using non-biodegradable polymers and organic solvents, causing the problems of removal after therapy and the suspected biotoxicity associated with residual solvent. Alginate (SA) was utilized in this investigation as the main material for electrospinning because of being biodegradable and water-soluble. The alginate-based electrospun mats were then treated by an ionic or a covalent crosslinking method, and then followed by chelation with Fe(2+)/Fe(3+) for chemical coprecipitation of Fe3O4 magnetic nanoparticles. Significant less cytotoxicity was noted on both liquid extracts from the ionic-crosslinked (Fe3O4-SA/PEO) and covalent-crosslinked (Fe3O4-SA/PVA) magnetic electrospun mats as well as the surface of Fe3O4-SA/PVA. In vitro hyperthermia assay indicated that the covalent-crosslinked magnetic alginate-based mats reduced tumor cell viability greater than Fe3O4 nanoparticles. Such magnetic electrospun mats are of potential for hyperthermia treatment by endoscopic/surgical delivery as well as serving as a supplementary debridement treatment after surgical tumor removal.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
authors.forename: Yen-Hsuan
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Division of Pediatric Nephrology, Department of Pediatric, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan; College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.
authors.forename: Chi-Hui
authors.initials: CH
authors.lastname: Cheng
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
authors.forename: Wan-Ju
authors.initials: WJ
authors.lastname: Chang
authors.affiliation: General Education Center and Department of Physical Therapy, Fooyin University, Kaohsiung 83102, Taiwan.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei 10051, Taiwan.
authors.forename: Feng-Huei
authors.initials: FH
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan; Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan. Electronic address: jclin@mail.ncku.edu.tw.
authors.forename: Jui-Che
authors.initials: JC
authors.lastname: Lin
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006979:Hyperthermia, Induced; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D058185:Magnetite Nanoparticles; D020723:Glucuronic Acid
keywords: Alginate; Electrospinning; Hyperthermia; Magnetic electrospun mats; Magnetic nanoparticles