title: Chicken gga-miR-103-3p Targets CCNE1 and TFDP2 and Inhibits MDCC-MSB1 Cell Migration.
abstract: Marek's disease (MD) is a highly contagious viral neoplastic disease caused by Marek's disease virus (MDV), which can lead to huge economic losses in the poultry industry. Recently, microRNAs (miRNAs) have been found in various cancers and tumors. In recent years, 994 mature miRNAs have been identified through deep sequencing in chickens, but only a few miRNAs have been investigated further in terms of their function. Previously, gga-miR-103-3p was found downregulated in MDV-infected samples by using Solexa deep sequencing. In this study, we further verified the expression of gga-miR-103-3p among MDV-infected spleen, MD lymphoma from liver, noninfected spleen, and noninfected liver, by qPCR. The results showed that the expression of gga-miR-103-3p was decreased in MDV-infected tissues, which was consistent with our previous study. Furthermore, two target genes of gga-miR-103-3p, cyclin E1 (CCNE1) and transcription factor Dp-2 (E2F dimerization partner 2) (TFDP2), were predicted and validated by luciferase reporter assay, qPCR, and western blot analysis. The results suggested that CCNE1 and TFDP2 are direct targets of gga-miR-103-3p in chickens. Subsequent cell proliferation and migration assay showed that gga-miR-103-3p suppressed MDCC-MSB1 migration, but did not obviously modulate MDCC-MSB1 cell proliferation. In conclusion, gga-miR-103-3p targets the CCNE1 and TFDP2 genes, and suppresses cell migration, which indicates that it might play an important role in MD tumor transformation.
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Han
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Lian
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Lujiang
authors.initials: L
authors.lastname: Qu
authors.affiliation: Division of Avian Infectious Diseases, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742.
authors.forename: Jiuzhou
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China nyang@cau.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D002645:Chickens / Q000235:genetics*; D019927:Cyclin E / Q000235:genetics*; D015870:Gene Expression; D008381:Herpesvirus 2, Gallid / Q000502:physiology; D006801:Humans; D008380:Marek Disease / Q000209:etiology; D035683:MicroRNAs / Q000235:genetics*; D009154:Mutation; D011201:Poultry Diseases / Q000235:genetics; D034622:RNA Interference; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D019927:Cyclin E; D035683:MicroRNAs; D014157:Transcription Factors
keywords: CCNE1; Marek’s disease; TFDP2; cell migration; chicken; gga-miR-103-3p