title: An Easy-to-Use Polystyrene Microchip-based Cell Culture System.
abstract: In this study, we developed an integrated, low-cost microfluidic cell culture system that is easy to use. This system consists of a disposable polystyrene microchip, a polytetrafluoroethylene valve, an air bubble trap, and an indium tin oxide temperature controller. Valve pressure resistance was validated with a manometer to be 3 MPa. The trap protected against bubble contamination. The temperature controller enabled the culture of Macaca mulatta RF/6A 135 vascular endothelial cells, which are difficult to culture in glass microchips, without a CO2 incubator. We determined the optimal coating conditions for these cells and were able to achieve stable, confluent culture within 1 week. This practical system is suitable for low-cost screening and has potential applications as circulatory cell culture systems and research platforms in cell biology.
authors.affiliation: Institute of Microchemical Technology Co., Ltd.
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Tazawa
authors.forename: Shohei
authors.initials: S
authors.lastname: Sunaoshi
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kitamori
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Ohtani-Kaneko
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology*; D008253:Macaca mulatta; D046208:Microchip Analytical Procedures / Q000379:methods*; D011137:Polystyrenes / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry; D013696:Temperature; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D011137:Polystyrenes; D017971:Tin Compounds; C109984:indium tin oxide; D011138:Polytetrafluoroethylene