title: [EFFECT OF PULSE-PERIODIC CORONA DISCHARGE ON VIABILITY OF ESCHERICHIA COLI M17 CELLS IN BIOFILMS].
abstract: OBJECTIVE
  Detection of bactericidal effect of pulse-periodic corona discharge (PPCD) on cells and biofilms of Escherichia coli M17.
  
  
  METHODS
  A gas-discharge device was created based on PPCD in air with power supply parameters: amplitude values of voltage of 30 - 60 kV, pulse repetition rate of 250 - 400 kHz. Ultrastructure changes in cells and biofilms of E. coli M17, affected by PPCD, generated in air, were studied by typical methods of transmission electron microscopy.
  
  
  RESULTS
  Disturbances of integrity of surface and abyssal structures of biofilms, as well as changes of morphological properties of E. coli M17 cells, characteristic for sub-lethal heat impact, were detected. Destructive changes of bacterial cells were developed by formation of focal disturbance of cytoplasmic membrane, extension of periplasmic space, formation of globular structures, characteristic for heat effect, and destruction of cytoplasm.
  
  
  CONCLUSIONS
  Bactericidal effect of PPCD on E. coli M17 cells as part of biofilms was shown. Destructive morphological changes in cells and biofilms of E. coli M17 after the effect of PPCD were detected for the first time on electron-microscopic level.
authors.forename: O V
authors.initials: OV
authors.lastname: Rybalchenko
authors.forename: O M
authors.initials: OM
authors.lastname: Stepanova
authors.forename: O G
authors.initials: OG
authors.lastname: Orlova
authors.forename: A M
authors.initials: AM
authors.lastname: Astafiev
authors.forename: A A
authors.initials: AA
authors.lastname: Kudryavtsev
authors.forename: V V
authors.initials: VV
authors.lastname: Kapustina
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D004560:Electricity; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D006358:Hot Temperature; D046529:Microscopy, Electron, Transmission; D019897:Periplasm / Q000187:drug effects* / Q000648:ultrastructure; D058626:Plasma Gases / Q000494:pharmacology*
chemical_list: D058626:Plasma Gases