title: ADAM10 localization in temporomandibular joint disk with internal derangement: an ex vivo immunohistochemical study.
abstract: The purpose of this study was to determine the presence of ADAM10 in temporomandibular joint disk with internal derangement. Twenty-five paraffin blocks of displaced temporomandibular joint (TMJ) disk specimens from earlier investigations were retrieved from the archives of the University of Catania. Of these 16 had been removed from females and 9 from males; 11 with anterior disk displacement with reduction (ADDwR) and 14 with anterior disk displacement without reduction (ADDwoR). The sections were dehydrated, embedded in paraffin and cut. Then they were incubated in 0.3% H2O2/methanol and half of sections from each sample were incubated in diluted rabbit polyclonal anti-ADAM10 antibody. Then biotinylated anti-mouse/anti-rabbit IgG was applied to the sections, followed by avidin-biotin-perioxidase complex. The results were analyzed and the results were that ADAM10 was overexpressed in the posterior band of sections from patients with ADDwR compared to the other bands of both ADDwR and ADDwoR sections. Overexpression correlated with severe histopathological degeneration. We believe these results have the potential to provide insights into the pathogenesis of TMJ disk degeneration and to help design new therapeutic approaches targeting the proteolytic events that lead to tissue degeneration. Early therapeutic block of ADAM10 activity could succeed in limiting aggrecan-rich matrix breakdown without affecting normal physiology.
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, University of Catania, Catania, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Loreto
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, University of Catania, Catania, Italy.
authors.forename: Giovanni Paolo Salvatore
authors.initials: GP
authors.lastname: Chiarenza
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, University of Catania, Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Musumeci
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, University of Catania, Catania, Italy. Electronic address: pacastro@unict.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Castrogiovanni
authors.affiliation: Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, University of Catania, Catania, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Imbesi
authors.affiliation: Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ruggeri
authors.affiliation: Department of Oral Sciences, Oral Surgery, Marquette University, School of Dentistry, Milwaukee, WI, USA.
authors.forename: Luis Edoardo
authors.initials: LE
authors.lastname: Almeida
authors.affiliation: Department of Surgical Sciences, University of Catania, Policlinico Universitario, Catania, Italy.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Leonardi
mesh_terms: D000072197:ADAM10 Protein / Q000378:metabolism*; D000328:Adult; D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009928:Organ Specificity; D019224:Temporomandibular Joint Disc / Q000201:enzymology* / Q000473:pathology; D013705:Temporomandibular Joint Disorders / Q000201:enzymology*
chemical_list: D008565:Membrane Proteins; D053829:Amyloid Precursor Protein Secretases; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human
keywords: ADAM10; ADDwR; ADDwoR; Immunohistochemistry; TMDs