title: Changes in autophagy in rats after spinal cord injury and the effect of hyperbaric oxygen on autophagy.
abstract: The purpose of this study was to explore the effects of Hyperbaric oxygen (HBO) on the autophagic changes after induction of spinal cord injury (SCI) in rats. A total of 75 rats were randomly divided into the sham-operated group, the spinal cord injury group, and the SCI+HBO group. We found that at 7 d and 14 d after surgery, the BBB scores were higher in the SCI+HBO group in comparison to the SCI group. The expression of Beclin-1 and LC3II was upregulated in the SCI and SCI+HBO groups after SCI. Fluorescently stained Beclin-1 and LC3II proteins were barely detectable in the sham group. In contrast, Beclin-l and LC3II expression was observed in neurons and glial cells from the SCI and SCI+HBO groups. Beclin-1 and LC3II expression appeared at 6h after SCI. At each time point, Beclin-1 and LC3II expression was significantly higher in the SCI+HBO group compared to the SCI group. These results suggest that autophagy is activated in rats after SCI and sustained over a period of time. HBO treatment enhances autophagy expression in rats after SCI and accelerates cell repair rate, which may represent one of the mechanisms of action of HBO in the treatment of SCI.
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Soochow University, Jiangsu 215004, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Soochow University, Jiangsu 215004, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Soochow University, Jiangsu 215004, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Su
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Soochow University, Jiangsu 215004, China.
authors.forename: Fanguo
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Anesthesiology, Suzhou BenQ Medical Center, Nanjing Medical University, Suzhou 215009, China. Electronic address: ably.tang@benqmedicalcenter.com.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Tang
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy; D000071186:Beclin-1; D005260:Female; D006931:Hyperbaric Oxygenation; D008124:Locomotion; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; D008869:Microtubule-Associated Proteins; C512829:Becn1 protein, rat; C526109:Anxa3 protein, rat
keywords: Autophagy; Beclin-1; Hyperbaric oxygen; LC3; Spinal cord injury