title: Capillary microfluidics-derived doxorubicin-containing human serum albumin microbeads for transarterial chemoembolization of hepatic cancer.
abstract: In this study, we prepared doxorubicin (DOXO)-loaded albumin microbeads (DOXO-MBs) using a capillary microfluidic device for transarterial chemoembolization of hepatic cancer. Albumin droplets were fabricated using the capillary microfluidic device and solidified by addition of glutaraldehyde. The acquired DOXO-MBs were homogeneous and the size was adjustable from 183.2 ± 12.2 μm to 351.5 ± 7.9 μm by changing the flow rate of fluidic solutions. The loading amount of DOXO was 9.7 ± 1.5 mg/g, and over 15.7% of DOXO was released over one month in pH7.2 buffer. Intra-portal injection of DOXO-MBs on normal liver of rats proved microbeads efficiently embolized hepatic vessels. Hepatic lobes, recovered 24 days after intra-portal injection, showed that the DOXO-MBs remained in hepatic vessels and released DOXO to surrounding hepatic tissues. In the hepatic tumor xenograft mouse model, DOXO-MBs inhibited tumor growth more efficiently than intravenous (I.V.) injection of free DOXO (p<0.01).
authors.affiliation: Department of New Drug Development, School of Medicine, Inha University, Incheon 400-712, Republic of Korea.
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Division of Gastroenterology & Hepatology, Inha University Hospital, Incheon 400-712, Republic of Korea.
authors.forename: Min A
authors.initials: MA
authors.lastname: Kim
authors.affiliation: Department of New Drug Development, School of Medicine, Inha University, Incheon 400-712, Republic of Korea.
authors.forename: Ratchapol
authors.initials: R
authors.lastname: Jenjob
authors.affiliation: Department of New Drug Development, School of Medicine, Inha University, Incheon 400-712, Republic of Korea; Division of Gastroenterology & Hepatology, Inha University Hospital, Incheon 400-712, Republic of Korea.
authors.forename: Don-Haeng
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of New Drug Development, School of Medicine, Inha University, Incheon 400-712, Republic of Korea. Electronic address: Sugeun.Yang@Inha.ac.kr.
authors.forename: Su-Geun
authors.initials: SG
authors.lastname: Yang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016461:Chemoembolization, Therapeutic / Q000379:methods*; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D046210:Microfluidic Analytical Techniques; D008863:Microspheres; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays
chemical_list: D012709:Serum Albumin; D004317:Doxorubicin
keywords: Albumin microparticles; Capillary microfluidics; Doxorubicin; Hepatocellular carcinoma; Transarterial chemoembolization