title: The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2.
abstract: The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on Group IA sPLA2. M. indica extract, dose dependently inhibited the GIA sPLA2 (NN-XIa-PLA2) activity with an IC50 value 8.1 µg/ml. M. indica extract effectively inhibited the indirect hemolytic activity up to 98% at ~40 µg/ml concentration and at various concentrations (0-50 µg/ml), it dose dependently inhibited the edema formation. When examined as a function of increased substrate and calcium concentration, there was no relieve of inhibitory effect on the GIA sPLA2. Furthermore, the inhibition was irreversible as evidenced from binding studies. It is observed that the aqueous extract ofM. indica effectively inhibits sPLA2 and it is associated inflammatory activities, which substantiate their anti-inflammatory properties. The mode of inhibition could be due to direct interaction of components present in the extract, with sPLA2 enzyme. Further studies on understanding the principal constituents, responsible for the anti-inflammatory activity would be interesting to develop this into potent anti-inflammatory agent.
authors.affiliation: Venom Research Unit, Adichunchanagiri Biotechnology and Cancer Research Institute, Karnataka, India.
authors.forename: Bhadrapura Lakkappa
authors.initials: BL
authors.lastname: Dhananjaya
authors.affiliation: Venom Research Unit, Adichunchanagiri Biotechnology and Cancer Research Institute, Karnataka, India.
authors.forename: Sudharshan
authors.initials: S
authors.lastname: Shivalingaiah
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy*; D054501:Group II Phospholipases A2 / Q000037:antagonists & inhibitors*; D006461:Hemolysis / Q000187:drug effects; D020128:Inhibitory Concentration 50; D031022:Mangifera / Q000737:chemistry* / Q000145:classification; D051379:Mice; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D054501:Group II Phospholipases A2