title: MicroRNA-133a inhibits the malignant behavior of glioma via downregulation of matrix metallopeptidase 9.
abstract: MicroRNA (miR)-133a expression has been reported to be downregulated in numerous human malignancies. However, the expression levels and function of miR-133a have not yet been investigated in human glioma. In the present study, the expression of miR‑133a was analyzed by reverse transcription‑quantitative polymerase chain reaction. Following transfection of miR‑133a, cell proliferation, cell migration, cell invasion and luciferase assays, and western blot analysis were conducted in glioma cell lines. The present study demonstrated that miR‑133a was downregulated in human glioma tissues compared with in normal adjacent tissues. In addition, the results indicated that miR‑133a was likely to directly target matrix metallopeptidase 9 in glioma. These results suggest that miR-133a may be considered as a target for the treatment of human glioma.
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, Chongqing 400042, P.R. China.
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, The Affiliated Hospital of University of Electronic Science and Technology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610054, P.R. China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yan
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D020780:Matrix Metalloproteinase 9