title: Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf.
abstract: Four polysaccharide fractions were isolated from young barley leaves treated with or without pectinase followed by ethanol fractionation. Among the polysaccharide fractions, BLE-P isolated from pectinase digested with a high molecular weight had the most enhanced macrophage stimulatory activity, indicating that pectinase digestion of barley leaf is a useful method for enhancement of its activity. BLE-P was further purified by column chromatography to identify the chemical and structural properties. BLE-P-I eluted in void volume fraction showed potent macrophage stimulatory activity. Monosaccharide composition and linkage analysis indicated that at least three kinds of polysaccharide, that is, glucuronoarabinoxylan (GAX; 40-45%), rhamnogalacturonan-I (RG-I) with branching mainly involving a type II arabinogalactan (AG-II) side chain (30-35%), and linear glucan such as starch and cellulose (less than 10%) coexisted in BLE-P-I. Given the association with macrophage stimulatory activity, it is likely that the GAX and to the RG-I polysaccharide branched with an AG-II side chain may be important for expression of the activity in barley leaf.
authors.affiliation: Department of Integrated Biomedical and Life Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 136-701, Republic of Korea; Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieu-dong, Youngtong-gu, Suwon, Gyeonggi-do 443-760, Republic of Korea.
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieu-dong, Youngtong-gu, Suwon, Gyeonggi-do 443-760, Republic of Korea.
authors.forename: Bong-Shin
authors.initials: BS
authors.lastname: Kwak
authors.affiliation: Korea Food Research Institute, Sungnam, Gyeonggi-do 462-746, Republic of Korea.
authors.forename: Hee-Do
authors.initials: HD
authors.lastname: Hong
authors.affiliation: Department of Integrated Biomedical and Life Science, Korea University, Anam-ro 145, Seongbuk-gu, Seoul 136-701, Republic of Korea. Electronic address: suh1960@korea.ac.kr.
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Suh
authors.affiliation: Department of Food Science and Biotechnology, Kyonggi University, San 94-6, Ieu-dong, Youngtong-gu, Suwon, Gyeonggi-do 443-760, Republic of Korea. Electronic address: ksshin@kyonggi.ac.kr.
authors.forename: Kwang-Soon
authors.initials: KS
authors.lastname: Shin
mesh_terms: D000818:Animals; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001467:Hordeum / Q000737:chemistry*; D006868:Hydrolysis; D051379:Mice; D018515:Plant Leaves / Q000737:chemistry*; D011096:Polygalacturonase / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000067996:RAW 264.7 Cells
chemical_list: D006023:Glycoproteins; C074523:HGP43 protein, human; D011134:Polysaccharides; D011096:Polygalacturonase
keywords: Barley leaf; Immunostimulatory activity; Pectinase digestion