title: Toward improved phosphorus efficiency in monogastrics-interplay of serum, minerals, bone, and immune system after divergent dietary phosphorus supply in swine.
abstract: Phosphorus (P) is of vital importance for many aspects of metabolism, including bone mineralization, blood buffering, and energy utilization. In order to identify molecular routes affecting intrinsic P utilization, we address processes covering P intake, uptake, metabolism, and excretion. In particular, the interrelation of bone tissue and immune features is of interest to approximate P intake to animal's physiology and health status. German Landrace piglets received different levels of digestible phosphorus: recommended, higher, or lower amounts. At multiple time points, relevant serum parameters were analyzed and radiologic studies on bone characteristics were performed. Peripheral blood mononuclear cells were collected to assess differential gene expression. Dietary differences were reflected by serum phosphorus, calcium, parathyroid hormone, and vitamin D. Bone reorganization was persistently affected as shown by microstructural parameters, cathepsin K levels, and transcripts associated with bone formation. Moreover, blood expression patterns revealed a link to immune response, highlighting bidirectional loops comprising bone formation and immune features, where the receptor-activator of NF-κB ligand/receptor-activator of NF-κB kinase system may play a prominent role. The modulated P supplementation provoked considerable organismal plasticity. Genes found to be differentially expressed due to variable P supply are involved in pathways relevant to P utilization and are potential candidate genes for improved P efficiency.
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Dummerstorf, Germany;
authors.forename: Michael
authors.initials: M
authors.lastname: Oster
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Dummerstorf, Germany;
authors.forename: Franziska
authors.initials: F
authors.lastname: Just
authors.affiliation: University of Rostock, Nutrition Physiology and Animal Nutrition, Rostock, Germany; and.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Büsing
authors.affiliation: University of Rostock, Nutrition Physiology and Animal Nutrition, Rostock, Germany; and.
authors.forename: Petra
authors.initials: P
authors.lastname: Wolf
authors.affiliation: University of Rostock, Institute for Experimental Surgery, Rostock, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Polley
authors.affiliation: University of Rostock, Institute for Experimental Surgery, Rostock, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Vollmar
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Dummerstorf, Germany;
authors.forename: Eduard
authors.initials: E
authors.lastname: Muráni
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Dummerstorf, Germany;
authors.forename: Siriluck
authors.initials: S
authors.lastname: Ponsuksili
authors.affiliation: Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Dummerstorf, Germany; wimmers@fbn-dummerstorf.de.
authors.forename: Klaus
authors.identifier: 0000-0002-9523-6790
authors.initials: K
authors.lastname: Wimmers
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015519:Bone Density; D001842:Bone and Bones / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D010758:Phosphorus / Q000378:metabolism*; D016226:Phosphorus, Dietary / Q000008:administration & dosage*; D013552:Swine; D059467:Transcriptome
chemical_list: D016226:Phosphorus, Dietary; D010758:Phosphorus
keywords: bone characteristics; diet; gene expression; peripheral blood mononuclear cell transcriptome; phosphorus