title: Therapeutic effects of various methods of MSC transplantation on cerebral resuscitation following cardiac arrest in rats.
abstract: In the present study, mesenchymal stem cells (MSCs) were transplanted into the brain of rats following cardiopulmonary resuscitation (CPR) by three different methods: Direct stereotaxic injection into the lateral cerebral ventricle (LV), intra‑carotid administration (A), and femoral venous infusion (V). The three different methods were compared by observing the effects of MSCs on neurological function following global cerebral hypoxia‑ischemia, in order to determine the optimum method for MSC transplantation. MSCs were transplanted in groups A, V and LV following the restoration of spontaneous circulation. Neurological deficit scale scores were higher in the transplantation groups, as compared with the control group. Neuronal damage, brain water content and serum levels of S100 calcium‑binding protein B were reduced in the hippocampus and temporal cortex of the transplantation groups, as compared with the control rats following resuscitation. MSCs were able to migrate inside the brain tissue following transplantation, and were predominantly distributed in the hippocampus and temporal cortex where the neurons were vulnerable during global cerebral ischemia. These results suggest that transplantation of MSCs may notably improve neurological function following CPR in a rat model. Of the three different methods of MSC transplantation tested in the present study, LV induced the highest concentration of MSCs in brain areas vulnerable to global cerebral ischemia, and therefore, produced the best neurological outcome.
authors.affiliation: Emergency Department, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Ka-Hong
authors.initials: KH
authors.lastname: Leong
authors.affiliation: Emergency Department, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhou
authors.affiliation: Emergency Department, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
authors.forename: Qing-Ming
authors.initials: QM
authors.lastname: Lin
authors.affiliation: Emergency Department, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Emergency Department, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yao
authors.affiliation: Emergency Department, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China.
authors.forename: Zi-Tong
authors.initials: ZT
authors.lastname: Huang
mesh_terms: D000818:Animals; D015415:Biomarkers; D001794:Blood Pressure; D018929:Cell Culture Techniques; D002465:Cell Movement; D002469:Cell Separation; D058948:Cell Tracking; D004195:Disease Models, Animal; D006323:Heart Arrest; D006339:Heart Rate; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D016130:Immunophenotyping; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D012151:Resuscitation / Q000379:methods; D013702:Temporal Lobe / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers