title: Three-dimensional imaging MS of lipids in atherosclerotic plaques: Open-source methods for reconstruction and analysis.
abstract: Three-dimensional MALDI imaging MS (IMS) is a growing branch of IMS still requiring developments in methodology and technology to make the technique routinely accessible. Many challenges are simply a matter of producing 3D reconstructions and interpreting them in a timely fashion. In this aim and using analysis of lipids from atherosclerotic plaques from a human carotid and mouse aortic sinuses, we describe 3D reconstruction methods using open-source software that provides high-quality visualization and rapid interpretation through multivariate segmentation of the 3D IMS data. Multiple datasets were generated for each sample and we provide insight into simple means to correlate the separate datasets.
authors.affiliation: Department of Chemistry, University of Montreal, Montreal, Quebec, Canada.
authors.forename: Nathan H
authors.initials: NH
authors.lastname: Patterson
authors.affiliation: Department of Medicine, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Doonan
authors.affiliation: Department of Medicine, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
authors.forename: Stella S
authors.initials: SS
authors.lastname: Daskalopoulou
authors.affiliation: Department of Chemistry, University of Montreal, Montreal, Quebec, Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Dufresne
authors.affiliation: Unit of Toxicology, University Centre of Legal Medicine, Geneva-Lausanne, Switzerland.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lenglet
authors.affiliation: First Clinic of Internal Medicine, Department of Internal Medicine, University of Genoa, Genoa, Italy.|Division of Cardiology, Foundation for Medical Researches, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Montecucco
authors.affiliation: Unit of Toxicology, University Centre of Legal Medicine, Geneva-Lausanne, Switzerland.|Faculty of Biology and Medicine, Lausanne University Hospital, University of Lausanne, Lausanne, Switzerland.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Thomas
authors.affiliation: Department of Chemistry, University of Montreal, Montreal, Quebec, Canada.
authors.forename: Pierre
authors.initials: P
authors.lastname: Chaurand
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000473:pathology; D002346:Carotid Sinus / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008055:Lipids / Q000302:isolation & purification*; D051379:Mice; D058226:Plaque, Atherosclerotic / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000473:pathology; D012850:Sinus of Valsalva / Q000000981:diagnostic imaging / Q000473:pathology
chemical_list: D008055:Lipids
keywords: 3D imaging; Atherosclerosis; Lipids; MALDI; MS; Technology