title: Nuclear distribution of eIF3g and its interacting nuclear proteins in breast cancer cells.
abstract: Eukaryotic translation initiation factor 3 subunit g (eIF3g) is a core subunit of the eukaryotic translation initiation factor 3 complex, and is important in the initiation of translation. It is also involved in caspase-mediated apoptosis, and is upregulated in multidrug-resistant cancer cells. In the present study, the nuclear distribution of eIF3g was determined by performing co-immunoprecipitation of proteins that potentially interact with eIF3g in the nucleus. Mass spectrometry characterization showed that three proteins, heterogeneous nuclear ribonucleoprotein U/scaffold attachment factor A, HSZFP36/zinc finger protein 823 and β‑actin, were among the candidate eIF3g‑interacting proteins in the nucleus. The protein‑protein interaction was further confirmed by cross‑linking and a glutathione S‑transferase pull‑down assay, followed by western blotting. The co‑localization of these proteins was determined by confocal microscopy. These findings provide novel insight into the possible functions of eIF3g in the nucleus and serves as an important first step for further investigation of the roles of eIF3g in cancer development.
authors.affiliation: Clinical Research Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Institute of Occupational Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310007, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Clinical Research Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Jingjia
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Clinical Laboratory, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Occupational Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310007, P.R. China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Clinical Research Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Cao
mesh_terms: D000199:Actins / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D039621:Eukaryotic Initiation Factor-3 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D034722:Heterogeneous-Nuclear Ribonucleoprotein U / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021381:Protein Transport
chemical_list: D000199:Actins; D039621:Eukaryotic Initiation Factor-3; D034722:Heterogeneous-Nuclear Ribonucleoprotein U; D009687:Nuclear Proteins