title: Wound healing property of isolated compounds from Boesenbergia kingii rhizomes.
abstract: BACKGROUND
  Boesenbergia kingii have been traditionally used in the treatment of inflammatory bowel disease, ulcerative colitis, aphthous ulcer, stomach discomfort, dysentery and abscess. Previously, we reported the B. kingii extract exert potential wound healing properties. Therefore the search of responsible constituents for wound healing property from these rhizomes is still relevant.
  
  
  OBJECTIVE
  This study was aimed to investigate for wound healing property of compounds from this plant in order to support its traditional uses.
  
  
  METHODS
  Wound healing activities were tested using in vitro assays including cell proliferation and migration assays, collagen production and H2O2-induced oxidative stress in mouse fibroblast L929 cells. The DPPH assay was also used to determine antioxidant activity.
  
  
  RESULTS
  Fourteen compounds from the chloroform fraction possessed potent anti-oxidant and wound healing activities. Compound 11 exhibited the most potent anti-DPPH effect (IC50=21.0µM) and also active against 0.5mMH2O2-induced oxidative stress by increasing cell survival ability up to 60.3% at 10µM. In addition, compounds 3, 8 and 14 at 10µM significantly enhanced L929 viability with 119.2%, 122.7% and 113.7%, respectively. Compounds 2, 7, 8 and 14 markedly enhanced L929 migration on day 2 up to 60-76% at 10µM, whereas 7 and 14 strongly stimulated collagen production at 75.0 and 96.7µg/ml compared to the control group (57.5µg/ml), respectively.
  
  
  CONCLUSIONS
  B. kingii is responsible for wound healing property via antioxidative effect, stimulation of fibroblast proliferation and migration as well as enhancement of collagen production.
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand; College of Oriental Medicine, Rangsit University, Pathumthani 12000, Thailand.
authors.forename: Teeratad
authors.initials: T
authors.lastname: Sudsai
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112, Thailand.
authors.forename: Chatchai
authors.initials: C
authors.lastname: Wattanapiromsakul
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla 90112, Thailand; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand. Electronic address: supinyat@yahoo.com.
authors.forename: Supinya
authors.initials: S
authors.lastname: Tewtrakul
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D006861:Hydrogen Peroxide; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*; D030521:Zingiberaceae
chemical_list: D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D003094:Collagen; D006861:Hydrogen Peroxide
keywords: Boesenbergia kingii; Diarylheptanoids; Flavonoids; L929 fibroblast cells; Sesquiterpenes; Wound healing