title: Topoisomerase II-associated protein PAT1H1 is involved in the root stem cell niche maintenance in Arabidopsis thaliana.
abstract: PAT1H1, one of the homologues of Topoisomerase II-associated protein, is involved in the maintenance of root stem cell niche through the interaction with NINJA. The root stem cell niche, which possesses four mitotically inactive quiescent cells (QC) and the surrounding mitotically active stem cells, is critical for root development in Arabidopsis thaliana. However, the molecular regulation of the maintenance of root stem cell niche identity is still not fully understood. Here we show that one of the homologues of Topoisomerase II-associated protein, here named as PAT1H1, could regulate root stem cell niche identity. The pat1h1 mutant showed higher frequency of QC cell division and root distal stem cell (DSC) differentiation. With a high expression in roots, PAT1H1 was found to interact with the jasmonic acid (JA) signalling negative regulator Novel Interactor of JAZ (NINJA) and thus regulate root DSC niche identity. Consistent with the active QC cell division, which rarely occurs in wild-type controls, the pat1h1 mutant displayed higher expression of CYCB1 in the root stem cell niche. Together our data reveals that PAT1H1 maintains root stem cell niche stability through the interaction with NINJA and the regulation of cell division.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Zheng
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Yuebin
authors.initials: Y
authors.lastname: Jia
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China. tianhuiyu@sdu.edu.cn.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Tian
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Ding
mesh_terms: D029681:Arabidopsis Proteins / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D004250:DNA Topoisomerases, Type II / Q000502:physiology*; D018517:Plant Roots / Q000166:cytology / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000502:physiology; D055153:Stem Cell Niche / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; C549252:NINJA protein, Arabidopsis; D012097:Repressor Proteins; D004250:DNA Topoisomerases, Type II
keywords: Root stem cell; Topoisomerase II-associated protein