title: Chronic Kidney Disease Impairs Bone Defect Healing in Rats.
abstract: Chronic kidney disease (CKD) has been regarded as a risk for bone health. The aim of this study was to evaluate the effect of CKD on bone defect repair in rats. Uremia was induced by subtotal renal ablation, and serum levels of BUN and PTH were significantly elevated four weeks after the second renal surgery. Calvarial defects of 5-mm diameter were created and implanted with or without deproteinized bovine bone mineral (DBBM). Micro-CT and histological analyses consistently revealed a decreased newly regenerated bone volume for CKD rats after 4 and 8 weeks. In addition, 1.4-mm-diameter cortical bone defects were established in the distal end of femora and filled with gelatin sponge. CKD rats exhibited significantly lower values of regenerated bone and bone mineral density (BMD) within the cortical gap after 2 and 4 weeks. Moreover, histomorphometric analysis showed an increase in both osteoblast number (N.Ob/B.Pm) and osteoclast number (N.Oc/B.Pm) in CKD groups due to hyperparathyroidism. Notably, collagen maturation was delayed in CKD rats as verified by Masson's Trichrome staining. These data indicate that declined renal function negatively affects bone regeneration in both calvarial and femoral defects.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.affiliation: Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
authors.forename: Dutmanee
authors.initials: D
authors.lastname: Seriwatanachai
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D017102:Fracture Healing; D050723:Fractures, Bone / Q000150:complications* / Q000503:physiopathology*; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D051436:Renal Insufficiency, Chronic / Q000150:complications*; D014511:Uremia / Q000150:complications; D055114:X-Ray Microtomography