title: Immunohistochemical expression of glucose transporter 1 in keratin-producing odontogenic cysts.
abstract: BACKGROUND
  Keratin-producing odontogenic cysts (KPOCs) are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. KPOCs included orthokeratinised odontogenic cyst (OOC) and parakeratotic odontogenic cysts, which are now considered true tumours denominated keratocystic odontogenic tumours (KCOTs). GLUT1 is a protein transporter that is involved in the active uptake of glucose across cell membranes and that is overexpressed in tumours in close correlation with the proliferation rate and positron emission tomography (PET) imaging results.
  
  
  METHODS
  A series of 58 keratin-producing odontogenic cysts was evaluated histologically and immunohistochemically in terms of GLUT1 expression. Different data were correlated using the beta regression model in relation to histological type and immunohistochemical expression of GLUT1, which was quantified using two different morphological methods.
  
  
  RESULTS
  KPOC cases comprised 12 OOCs and 46 KCOTs, the latter corresponding to 6 syndromic and 40 sporadic KCOTs. GLUT1 expression was very low in OOC cases compared with KCOT cases, with statistical significant differences when quantification was considered. Different GLUT1 localisation patterns were revealed by immunostaining, with the parabasal cells showing higher reactivity in KCOTs. However, among KCOTs cases, GLUT1 expression was unable to establish differences between syndromic and sporadic cases.
  
  
  CONCLUSIONS
  GLUT1 expression differentiated between OOC and KCOT cases, with significantly higher expression in KCOTs, but did not differentiate between syndromic and sporadic KCOT cases. However, given the structural characteristics of KCOTs, we hypothesised that PET imaging methodology is probably not a useful diagnostic tool for KCOTs. Further studies of GLUT1 expression and PET examination in KCOT series are needed to confirm this last hypothesis.
authors.affiliation: Department of Stomatology, University of Valencia, Valencia, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Vera-Sirera
authors.affiliation: Department of Stomatology, Endodontics Unit, University of Valencia, Valencia, Spain.
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Forner-Navarro
authors.affiliation: Department of Pathology, University of Valencia and La Fe University Hospital, Valencia, Spain. fco.jose.vera@uv.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Vera-Sempere
mesh_terms: D004847:Epithelial Cells / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism*; D009807:Odontogenic Cysts / Q000378:metabolism*; D009808:Odontogenic Tumors / Q000378:metabolism*
chemical_list: D051272:Glucose Transporter Type 1; C495677:SLC2A1 protein, human; D007633:Keratins
keywords: Glucose transporter protein; Immunohistochemistry; Keratin-producing odontogenic cyst; Keratocystic odontogenic tumour; Orthokeratinised odontogenic cyst; Positron emission tomography