title: On the interaction of alginate-based core-shell nanocarriers with keratinocytes in vitro.
abstract: Calcium alginate nanocarriers (CaANCs) were developed as a potential tool for delivery of hydrophobic active molecules such as pharmaceutical and cosmetic active ingredients. In this study, we focused on interactions between CaANCs and keratinocytes in culture and examined toxicity, internalization and drug release. Prior to cellular interactions, cryogenic transmission electron microscopy images showed that CaANCs appear as regular, spherical and dense particles, giving evidence of the surface gelation of CaANCs. Their size, around 200nm, was stable under tested conditions (temperature, culture media, presence of serum and presence of encapsulated dye), and their toxicity on keratinocytes was very low. Flow cytometry assays showed that CaANCs are internalized into keratinocytes by endocytosis with a predominant implication of the caveolae-mediated route. Förster resonance energy transfer (FRET) demonstrated that after a 2h contact, the release of CaANC contents in the cytoplasm of keratinocytes was almost complete. The endocytosis of CaANCs by a lysosome-free pathway, and the rapid release of their contents inside keratinocytes, will allow vectorized molecules to fully exhibit their pharmacological or cosmetic activity.
authors.affiliation: Université François Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37200 Tours, France.
authors.forename: Hoang Truc Phuong
authors.initials: HTP
authors.lastname: Nguyen
authors.affiliation: Université François Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37200 Tours, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Allard-Vannier
authors.affiliation: U.R. 1268 BIA Biopolymères Interactions Assemblages INRA, rue de la Géraudière, 44316 Nantes, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Gaillard
authors.affiliation: Université François Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37200 Tours, France.
authors.forename: Imane
authors.initials: I
authors.lastname: Eddaoudi
authors.affiliation: Université François Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37200 Tours, France.
authors.forename: Lynda
authors.initials: L
authors.lastname: Miloudi
authors.affiliation: Université François Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37200 Tours, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Soucé
authors.affiliation: Université François Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37200 Tours, France.
authors.forename: Igor
authors.initials: I
authors.lastname: Chourpa
authors.affiliation: Université François Rabelais de Tours, EA 6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37200 Tours, France. Electronic address: emilie.munnier@univ-tours.fr.
authors.forename: Emilie
authors.initials: E
authors.lastname: Munnier
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry; D021941:Caveolae / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D004339:Drug Compounding; D065546:Drug Liberation; D004705:Endocytosis; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size
chemical_list: D000464:Alginates; D002232:Carbocyanines; D004337:Drug Carriers; D005456:Fluorescent Dyes; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Alginate; Core-shell nanocarrier; Drug delivery; Endocytosis; Förster resonance energy transfer (FRET); Keratinocyte