title: Silver nanoparticles coated graphene electrochemical sensor for the ultrasensitive analysis of avian influenza virus H7.
abstract: A new, highly sensitive electrochemical immunosensor with a sandwich-type immunoassay format was designed to quantify avian influenza virus H7 (AIV H7) by using silver nanoparticle-graphene (AgNPs-G) as trace labels in clinical immunoassays. The device consists of a gold electrode coated with gold nanoparticle-graphene nanocomposites (AuNPs-G), the gold nanoparticle surface of which can be further modified with H7-monoclonal antibodies (MAbs). The immunoassay was performed with H7-polyclonal antibodies (PAbs) that were attached to the AgNPs-G surface (PAb-AgNPs-G). This method of using PAb-AgNPs-G as detection antibodies shows high signal amplification and exhibits a dynamic working range of 1.6 × 10(-3)∼16 ng/mL, with a low detection limit of 1.6 pg/mL at a signal-to-noise ratio of 3σ. In summary, we showed that this novel immunosensor is highly specific and sensitive to AIV H7, and the established assay could potentially be applied to rapidly detect other pathogenic microorganisms.
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Jiaoling
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China. Electronic address: xiezhixun@126.com.
authors.forename: Zhixun
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Luo
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Liji
authors.initials: L
authors.lastname: Xie
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zeng
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001717:Birds / Q000821:virology; D055664:Electrochemical Techniques / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry*; D009980:Influenza A virus / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*
chemical_list: D012834:Silver; D006108:Graphite
keywords: Avian influenza virus H7; Electrochemical immunosensor; Graphene; Nanolabel; Sensitivity enhancement