title: Ibuprofen-conjugated hyaluronate/polygalacturonic acid hydrogel for the prevention of epidural fibrosis.
abstract: The formation of fibrous tissue is part of the natural healing response following a laminectomy. Severe scar tissue adhesion, known as epidural fibrosis, is a common cause of failed back surgery syndrome. In this study, by combining the advantages of drug treatment with a physical barrier, an ibuprofen-conjugated crosslinkable polygalacturonic acid and hyaluronic acid hydrogel was developed for epidural fibrosis prevention. Conjugation was confirmed and measured by 1D(1)H NMR spectroscopy.In vitroanalysis showed that the ibuprofen-conjugated polygalacturonic acid-hyaluronic acid hydrogel showed low cytotoxicity. In addition, the conjugated ibuprofen decreased prostaglandin E2production of the lipopolysaccharide-induced RAW264.7 cells. Histological data inin vivostudies indicated that the scar tissue adhesion of laminectomized male adult rats was reduced by the application of our ibuprofen-conjugated polygalacturonic acid-hyaluronic acid hydrogel. Its use also reduced the population of giant cells and collagen deposition of scar tissue without inducing extensive cell recruitment. The results of this study therefore suggest that the local delivery of ibuprofenviaa polygalacturonic acid-hyaluronic acid-based hydrogel reduces the possibility of epidural fibrosis.
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Hsiu-Hui
authors.initials: HH
authors.lastname: Peng
authors.affiliation: Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
authors.forename: Mei-Hsiu
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, National Taiwan University Hospital.
authors.forename: Jui-Sheng
authors.initials: JS
authors.lastname: Sun
authors.affiliation: Division of Neurosurgery, Department of Surgery, Cathay General Hospital, Taipei, Taiwan School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
authors.forename: Chih-Ju
authors.initials: CJ
authors.lastname: Chang
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Tse-Ying
authors.initials: TY
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan Division of Neurosurgery, Department of Surgery, Cathay General Hospital, Taipei, Taiwan School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan Department of Biomedical Engineering, Ming Chuan University, Taipei, Taiwan chen.minghong@gmail.com tyliu5@ym.edu.tw.
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Chen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage* / Q000627:therapeutic use; D002921:Cicatrix / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D004337:Drug Carriers / Q000737:chemistry; D004824:Epidural Space / Q000187:drug effects* / Q000473:pathology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D007052:Ibuprofen / Q000008:administration & dosage* / Q000627:therapeutic use; D007796:Laminectomy / Q000009:adverse effects*; D008297:Male; D051379:Mice; D010368:Pectins / Q000737:chemistry*; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control; D000067996:RAW 264.7 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004337:Drug Carriers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D010368:Pectins; C003181:polygalacturonic acid; D007052:Ibuprofen
keywords: Epidural fibrosis; hyaluronic acid; ibuprofen; injectable hydrogel; polygalacturonic acid; prostaglandin E2