title: Oxidative stress in hoof laminar tissue of horses with lethal gastrointestinal diseases.
abstract: Tissue damage caused by oxidative stress is involved in the pathogenesis of several diseases in animals and man, and is believed to play a role in the development of laminitis in horses. The aim of this study was to investigate the oxidative stress associated with laminar lesions in horses with lethal gastrointestinal disorders. Laminar tissue samples of the hoof of 30 horses were used. Tissue samples were divided as follows: six healthy horses (control group-CG), and 24 horses that died after complications of gastrointestinal diseases (group suffering from gastrointestinal disorders-GDG). Superoxide dismutase (SOD2) and nitrotyrosine immunostaining and the severity of laminar lesions were evaluated. Presence of laminar lesions and immunostaining for nitrotyrosine and SOD2 were only evident in horses from the GDG group. Thus, oxidative stress may play a role in the pathogenesis of laminar lesions secondary to gastrointestinal disorders.
authors.affiliation: Graduate School in Veterinary Sciences, Federal University of Paraná (Curitiba campus), Rua dos Funcionários, 1540, Curitiba, Paraná 80035-050, Brazil. Electronic address: luci.laskoski@gmail.com.
authors.forename: Luciane Maria
authors.initials: LM
authors.lastname: Laskoski
authors.affiliation: Graduate School in Veterinary Sciences, Federal University of Paraná (Curitiba campus), Rua dos Funcionários, 1540, Curitiba, Paraná 80035-050, Brazil. Electronic address: roslocdi@ufpr.br.
authors.forename: Rosangela Locatelli
authors.initials: RL
authors.lastname: Dittrich
authors.affiliation: Graduate School in Veterinary Surgery, São Paulo State University Júlio de Mesquita Filho - UNESP (Jaboticabal campus), Via de Acesso Professor Paulo Donato Castellane, s/n, Jaboticabal, São Paulo 14884-900, Brazil. Electronic address: valadao@fcav.unesp.br.
authors.forename: Carlos Augusto Araújo
authors.initials: CA
authors.lastname: Valadão
authors.affiliation: Graduate School in Veterinary Sciences, Federal University of Paraná (Curitiba campus), Rua dos Funcionários, 1540, Curitiba, Paraná 80035-050, Brazil. Electronic address: julianasbrum@yahoo.com.br.
authors.forename: Juliana Sperotto
authors.initials: JS
authors.lastname: Brum
authors.affiliation: Graduate School in Microbiology, Parasitology and Patology, Federal University of Paraná (Curitiba campus), Avenida Coronel Francisco H. dos Santos, s/n, Curitiba, Paraná 81530-900, Brazil. Electronic address: yara_brandao@hotmail.com.
authors.forename: Yara
authors.initials: Y
authors.lastname: Brandão
authors.affiliation: Graduate School in Veterinary Sciences, Federal University of Paraná (Curitiba campus), Rua dos Funcionários, 1540, Curitiba, Paraná 80035-050, Brazil. Electronic address: haraldvet@yahoo.com.br.
authors.forename: Harald Fernando Vicente
authors.initials: HF
authors.lastname: Brito
authors.affiliation: Department of Veterinary Medicine, Federal University of Paraná (Curitiba campus), Rua dos Funcionários, 1540, Curitiba, Paraná 80035-050, Brazil. Electronic address: renatosousa@ufpr.br.
authors.forename: Renato Silva
authors.initials: RS
authors.lastname: de Sousa
mesh_terms: D000818:Animals; D005260:Female; D005534:Foot Diseases / Q000473:pathology / Q000662:veterinary*; D005767:Gastrointestinal Diseases / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D006724:Hoof and Claw / Q000473:pathology*; D006734:Horse Diseases / Q000401:mortality / Q000473:pathology*; D006736:Horses; D008297:Male; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: C002744:3-nitrotyrosine; D014443:Tyrosine; D013482:Superoxide Dismutase
keywords: Equine; Immunohistochemistry; Laminitis; Nitrotyrosine; Superoxide dismutase