title: Polyomavirus infections and its clinical relevance in cancer patients: A Prospective Study.
abstract: BK and JC polyomaviruses (PyV) have been demonstrated to be associated with the pathogenesis of various human cancers. We aimed to investigate the impact of BK and JC polyomavirus infections on several clinical parameters in different human cancers. A total of 150 cancer patients were included in the study (51 patients with solid tumors, 48 patients with lymphomas and 51 patients with leukemias). Amplification of PyV DNA was performed using a semi-nested version of Polymerase chain reaction targeting the T genomic region of PyV. The polyomavirus load was determined using real-time PCR assay. The clinical data were collected. Polyomavirus DNA could be detected in 84 (56%) of 150 of all cancerous patients. The solid tumors had the lowest proportion of JCV (6 (11.8%) of 51), whereas had the highest proportion of JCV (200copies/μl). JCV was more frequent among NHL patients (30%) and absent in HL patients (0%). During follow-up, PyV positivity decreased significantly (p=0.004) in lymphoma patients (n=28). Although PyV positivity decreased significantly from 39% to 7% in 28 of 48 lymphoma patients after treatment, it significantly persisted in leukemic patients after treatment (from 22% to 38%). JC was more frequent among leukemic patients with leukopenia. The presence of JC polyomavirus was more frequent among leukemic patients without any significant impact on their overall survival.
authors.affiliation: Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt. Electronic address: samaly183@yahoo.com.
authors.forename: Samah A
authors.initials: SA
authors.lastname: Loutfy
authors.affiliation: Biostatistics and Cancer Epidemiology Department, National Cancer Institute, Cairo University, Egypt.
authors.forename: Manar M
authors.initials: MM
authors.lastname: Moneer
authors.affiliation: Medical Oncology Department, National Cancer Institute, Cairo University, Egypt.
authors.forename: Salem E
authors.initials: SE
authors.lastname: Salem
authors.affiliation: Botany & Microbiology Department, Faculty of Science, Helwan University, Egypt.
authors.forename: Emad A
authors.initials: EA
authors.lastname: El-Moniem Abada
authors.affiliation: Botany & Microbiology Department, Faculty of Science, Helwan University, Egypt.
authors.forename: Entsar A
authors.initials: EA
authors.lastname: El-Moniem Ahmed
authors.affiliation: Botany & Microbiology Department, Faculty of Science, Helwan University, Egypt.
authors.forename: Lamiaa H
authors.initials: LH
authors.lastname: Ibrahim
authors.affiliation: Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Egypt.
authors.forename: El-Chaimaa B
authors.initials: EB
authors.lastname: Mohamed
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001739:BK Virus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007577:JC Virus / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000150:complications*; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000473:pathology; D015995:Prevalence; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D016019:Survival Analysis; D019562:Viral Load; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: BK and JC polyomavirus infections; Hematological malignancies; Overall survival; Real-time PCR