title: Facilitating best practices in collecting anomalous scattering data for de novo structure solution at the ESRF Structural Biology Beamlines.
abstract: The constant evolution of synchrotron structural biology beamlines, the viability of screening protein crystals for a wide range of heavy-atom derivatives, the advent of efficient protein labelling and the availability of automatic data-processing and structure-solution pipelines have combined to make de novo structure solution in macromolecular crystallography a less arduous task. Nevertheless, the collection of diffraction data of sufficient quality for experimental phasing is still a difficult and crucial step. Here, some examples of good data-collection practice for projects requiring experimental phasing are presented and recent developments at the ESRF Structural Biology beamlines that have facilitated these are illustrated.
authors.affiliation: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Daniele
authors.initials: D
authors.lastname: de Sanctis
authors.affiliation: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Marcus
authors.initials: M
authors.lastname: Oscarsson
authors.affiliation: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Alexander
authors.initials: A
authors.lastname: Popov
authors.affiliation: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Olof
authors.initials: O
authors.lastname: Svensson
authors.affiliation: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Gordon
authors.initials: G
authors.lastname: Leonard
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D003625:Data Collection / Q000295:instrumentation / Q000379:methods; D001330:Electronic Data Processing / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D011506:Proteins / Q000737:chemistry*; D012984:Software; D017356:Synchrotrons
chemical_list: D011506:Proteins
keywords: ESRF; MAD; MXCuBE2; experimental phasing