title: Factors that Affect Pancreatic Islet Cell Autophagy in Adult Rats: Evaluation of a Calorie-Restricted Diet and a High-Fat Diet.
abstract: Aging may be a risk factor for type 2 diabetes in the elderly. Dietary intervention can affect glucose tolerance in adults, which may be due to body composition and islet cell autophagy. The aim of this study was to determine the effects of various dietary interventions on islet cell autophagy. Pancreatic tissue and blood samples were collected from Sprague Dawley rats (14-16 months old, n = 15 for each group) that received a normal diet (ND), a high-fat diet (HFD), or a calorie-restricted diet (CRD). The body weight (BW), visceral fat, serum lipid levels, fasting serum glucose, insulin levels, and β/α cell area were determined in 14-16-(0-w), 16-18-(8-w), and 18-20(16-w)-month-old rats. Pancreatic islet autophagy (LC3B and LAMP2), AP (Acid Phosphatase) and apoptosis (apoptosis index, AI (TUNEL assay) and cleaved caspase-3) were detected using immunohistochemistry, ELISA and western blot. At 16 weeks, the expressions of LC3B, LAMP2 and AP markedly increased in both the HFD (P<0.01) and CRD (P<0.05) groups; however, an increase in the AI (P<0.05), cleaved caspase-3 and Beclin1 expression and a decrease in the expressions of BCL2 and BCLXL (P<0.05) were observed in only the HFD group. FFA, triglyceride levels, HOMA-IR, insulin levels and glucagon levels were significantly increased in the HFD group but decreased in the CRD group at 16 weeks (P<0.05). The degree of islet cell autophagy was potentially regulated by the levels of FFA and islet cell insulin and glucagon, which may have been due to the effects of Beclin1/BCL2.
authors.affiliation: The Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: The Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Nie
authors.affiliation: The Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Lingxiao
authors.initials: L
authors.lastname: Wang
authors.affiliation: The Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: The Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Zhangming
authors.initials: Z
authors.lastname: Meng
authors.affiliation: Laboratory of Aging Research, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Hengyi
authors.initials: H
authors.lastname: Xiao
authors.affiliation: The Department of Hematology, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Xiang
authors.affiliation: The Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Xiujun
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan province, China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Fu
authors.affiliation: The Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan province, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001343:Autophagy; D001786:Blood Glucose; D001835:Body Weight; D031204:Caloric Restriction; D016922:Cellular Senescence; D059305:Diet, High-Fat; D020287:In Situ Nick-End Labeling; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat; D007515:Islets of Langerhans / Q000473:pathology*; D050356:Lipid Metabolism; D052119:Lysosomal-Associated Membrane Protein 2 / Q000378:metabolism; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012307:Risk Factors
chemical_list: D000135:Acid Phosphatase; D001786:Blood Glucose; D007328:Insulin; D052119:Lysosomal-Associated Membrane Protein 2; D008869:Microtubule-Associated Proteins; C526109:Anxa3 protein, rat