title: Melting curve analysis in canine lymphoma by calculating maximum fluorescence decrease.
abstract: PARR is widely used in the diagnostics of canine lymphoma. In human and veterinary medicine, melting curve analysis (MCA) has successfully been introduced to facilitate the process. Since visual interpretation of melting curves can be rather subjective, the purpose of this study was to develop an objective interpretation of melting curves by calculating the maximum fluorescence decrease (dFmax) within a defined rise of temperature. Lymph node aspirates and blood of 34 dogs with lymphoma and 28 control dogs were tested. 27/34 lymphoma cases were correctly detected to be monoclonal (sensitivity 79%). 2/28 control dogs showed a monoclonal rearrangement (specificity 93%). B- and T-cell neoplasia were still detectable using DNA amount as low as 10 ng. In serial dilutions of tumor DNA with DNA of normal tonsils, the detection limit was 25% for B-cell lymphomas and 100% for T-cell lymphoma, suggesting that PCR conditions could still be optimized.
authors.affiliation: Clinic of Small Animal Medicine, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Schöpper
authors.affiliation: Clinic of Small Animal Medicine, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ohmura
authors.affiliation: Department of Pathobiology, Clinical Pathology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Rütgen
authors.affiliation: Department of Veterinary Internal Medicine, Graduate School of Agricultural Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tsujimoto
authors.affiliation: Clinic of Small Animal Medicine, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Weber
authors.affiliation: Clinic of Small Animal Medicine, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hirschberger
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D004273:DNA, Neoplasm / Q000737:chemistry; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D005453:Fluorescence; D008198:Lymph Nodes / Q000473:pathology; D008223:Lymphoma / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000662:veterinary; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000662:veterinary; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary
chemical_list: D004273:DNA, Neoplasm
keywords: Canine lymphoma; PARR; maximum fluorescence decrease; melting curve analysis