title: Functional Role of FcγRIIB in the Regulation of Mesenchymal Stem Cell Function.
abstract: Mesenchymal stem cells (MSCs) derived from bone marrow are plural-potent stem cells with immune regulatory functions. We aimed to evaluate role of FcγRIIB in the regulation of bone marrow-derived MSC function. MSCs were prepared from mouse bone marrow derived from wild-type (WT) or FcγRIIB-deficient (FcγRIIB-/-) mice. MSCs were co-cultured with bone marrow-derived dendritic cells (BMDCs), and BMDC maturation and function were evaluated by flow cytometric analysis and carboxyfluorescein succinimidyl ester-labeled OT-II T-cell addition. An acute asthma model was established by aeresol ovalbumin challenge in mice. Mice received WT or FcγRIIB-/- MSC therapy. Lung function was evaluated by histological examination and cytokine production measurement. mRNA and protein expression levels of target genes were examined by real-time quantitative polymerase chain reactionor western blotting. We found that MSCs derived from bone marrow exhibit a high level of FcγRIIB expression. FcγRIIB deficiency impaired the suppressive function of MSCs, as FcγRIIB deficiency efficiently reversed the inhibitory effect of MSCs on BMDC maturation and function. Additionally, FcγRIIB-/-MSCs were less potent at suppressing asthma in model mice, possibly through reduced expression of Smad2, Smad3, Cox-2, and prostaglandin E2 in FcγRIIB-/-MSCs. FcγRIIB might play an essential role in regulating the inhibitory effects of MSCs derived from bone marrow.
authors.affiliation: 1. Department of Respiratory, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;; 2. Department of Respiratory, The General Hospital of Shenyang Military, Shenyang, Liaoning, 110015, China;
authors.forename: Tianyi
authors.initials: T
authors.lastname: Zhu
authors.affiliation: 3. Department of VIP Treatment, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
authors.forename: Ruohua
authors.initials: R
authors.lastname: Chen
authors.affiliation: 4. Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210000, China;
authors.forename: Zeng
authors.initials: Z
authors.lastname: Li
authors.affiliation: 5. Department of Immunology, Zhejiang University, Hangzhou, Zhejiang310000, China;
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
authors.affiliation: 1. Department of Respiratory, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
authors.forename: Changwen
authors.initials: C
authors.lastname: Deng
authors.affiliation: 1. Department of Respiratory, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
authors.forename: Xingxing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 6. Department of Respiratory, No. 1 People's Hospital of Yancheng, Yancheng, Jiangsu, 224000, China;
authors.forename: Koudong
authors.initials: K
authors.lastname: Zhang
authors.affiliation: 7. Department of Respiratory, The 174 Hospital, Xiamen, Fujian, 361000, China.
authors.forename: Linrong
authors.initials: L
authors.lastname: Tong
authors.affiliation: 8. Institute of Immunology, The Second Military Medical University, Shanghai, 200433, China.
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Yu
authors.affiliation: 1. Department of Respiratory, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
authors.forename: Chong
authors.initials: C
authors.lastname: Bai
mesh_terms: D000818:Animals; D001249:Asthma / Q000209:etiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D051546:Cyclooxygenase 2 / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism*; D051899:Smad2 Protein / Q000378:metabolism; D051900:Smad3 Protein / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: C499172:Fcgr2b protein, mouse; D017452:Receptors, IgG; D051899:Smad2 Protein; C497783:Smad2 protein, mouse; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone
keywords: FcγRIIB; asthma.; dendritic cell maturation; mesenchymal stem cells