title: Key enzymes catalyzing glycerol to 1,3-propanediol.
abstract: Biodiesel can replace petroleum diesel as it is produced from animal fats and vegetable oils, and it produces about 10 % (w/w) glycerol, which is a promising new industrial microbial carbon, as a major by-product. One of the most potential applications of glycerol is its biotransformation to high value chemicals such as 1,3-propanediol (1,3-PD), dihydroxyacetone (DHA), succinic acid, etc., through microbial fermentation. Glycerol dehydratase, 1,3-propanediol dehydrogenase (1,3-propanediol-oxydoreductase), and glycerol dehydrogenase, which were encoded, respectively, by dhaB, dhaT, and dhaD and with DHA kinase are encompassed by the dha regulon, are the three key enzymes in glycerol bioconversion into 1,3-PD and DHA, and these are discussed in this review article. The summary of the main research direction of these three key enzyme and methods of glycerol bioconversion into 1,3-PD and DHA indicates their potential application in future enzymatic research and industrial production, especially in biodiesel industry.
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China ; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, 361005 China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China ; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, 361005 China.
authors.forename: Shizhen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China.
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China ; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, 361005 China ; The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, 361005 Fujian China.
authors.forename: Baishan
authors.initials: B
authors.lastname: Fang
keywords: Biocatalyst; Bioconversion; Biodiesel; GDH; GDHt; Glycerol; Industrial enzyme; Multienzyme coupling; PDOR; Renewable resources