title: Fractionated elution using the TEKCIS technetium-99m generator.
abstract: The TEKCIS technetium-99m (Tc) generator was designed to allow dry column shipment and automatized conception. A high Tc radioactive concentration is required in a subset of radiopharmacy procedures. Fractionated elution can be a useful tool to meet this requirement, especially when current elution is close to the generator expiration date. The aim of our study was to assess TEKCIS generator elution kinetics and to determine the optimal fractionated elution time to fit with procedures requiring the highest Tc radioactive concentration in clinical use. After duplicate elution at several predetermined elution times, the volume and activity of each eluate were measured. Two optimal time points were selected to perform fractionated elution and repeatability (n=34 and 33) assessed on TEKCIS generators calibrated at 6 or 8 GBq. The complete eluate volume (5 ml) was collected after 60 s of elution. A logarithmic equation was established between eluate volume (v, ml) from elapsed elution time (t, s): v=1.8335ln(t)-2.5965. Using the reciprocal equation, elution times required to obtain some commonly eluted volumes were calculated. Fractionated elutions during 15 and 20 s were selected and an average elution volume from 2.74 to 3.27 ml was collected, with an average elution yield of approximately 90 and 100%, respectively. Our work provides a simple and reliable methodology for the use of fractionated elution with the new TEKCIS generator.
authors.affiliation: Departments of aNuclear Medicine bPharmacy cUMR 1086 INSERM-UCBN, CHU de Caen dEA 4650 University of Caen-Basse Normandy, Caen F-14000, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Vigne
authors.forename: Rémy
authors.initials: R
authors.lastname: De Mil
authors.forename: Damien
authors.initials: D
authors.lastname: Peyronnet
authors.forename: Claudine
authors.initials: C
authors.lastname: Hecquard
authors.forename: Denis
authors.initials: D
authors.lastname: Agostini
authors.forename: Françoise
authors.initials: F
authors.lastname: Lemonnier
mesh_terms: D019583:Dose Fractionation, Radiation; D007553:Isotope Labeling / Q000295:instrumentation* / Q000379:methods; D007700:Kinetics; D011829:Radiation Dosage; D011874:Radiometry; D011876:Radionuclide Generators / Q000295:instrumentation*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D013667:Technetium / Q000737:chemistry*
chemical_list: D019275:Radiopharmaceuticals; D013667:Technetium