title: Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo.
abstract: Interleukin-24 (IL-24) displays cancer-specific apoptosis-inducing properties in a broad spectrum of human tumors without harmful effects on normal cells. The human IL-24 protein is secreted as a glycosylated protein and functions as a pro-Th1 cytokine and a potent antiangiogenic molecule. However, the function of secreted recombinant human IL-24 (srhIL-24) protein in esophageal squamous cell carcinoma (ESCC) cells has not been studied. In the present study, we prepared a stable site-specific-integrated cell line, Flp-InTMCHO/IL-24 (FCHO/IL-24), which secreted rhIL-24 at a higher level than three random-integrated cell lines. In vitro, we identified that the purified srhIL-24 inhibited proliferation and induced the apoptosis of ESCC Eca-109 cells and activated STAT3, which was related with the IL-20 receptors. In vivo, the tumorigenicity of Eca-109 cells was significantly inhibited by s.c. injection of FCHO/IL-24 cells. Decreased tumor microvessel density and an increased number of TUNEL-positive tumor cells were associated with tumor growth inhibition, indicating the presence of antiangiogenic activity and induction of apoptotic activity. In summary, the present study demonstrated that srhIL-24 induced growth inhibition and apoptosis in ESCC Eca-109 cells in vitro and in vivo, which may be mediated by the receptor pathway.
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of the Academy of Military Medical Sciences, Fengtai, Beijing 100071, P.R. China.
authors.forename: Qunfeng
authors.initials: Q
authors.lastname: Ma
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Bangming
authors.initials: B
authors.lastname: Jin
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Shi
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Pengkun
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Biological Diagnosis, Beijing Institute of Basic Medical Sciences, Haidian, Beijing 100850, P.R. China.
authors.forename: Cuimi
authors.initials: C
authors.lastname: Duan
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Heyu
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Affiliated Hospital of the Academy of Military Medical Sciences, Fengtai, Beijing 100071, P.R. China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Deng
authors.affiliation: Cell Engineering Research Center, The Fourth Military Medical University, Xicheng, Xi'an, Shaanxi 710032, P.R. China.
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Ziling
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Science and Bioengineering, School of Science, Beijing Jiaotong University, Haidian, Beijing 100044, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Life Science, Southwest University, Beibei, Chongqing 400715, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D016466:CHO Cells; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D004938:Esophageal Neoplasms; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007378:Interleukins / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D018123:Receptors, Interleukin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D000099180:Interleukin-24
chemical_list: D000970:Antineoplastic Agents; D007378:Interleukins; D018123:Receptors, Interleukin; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; D000099180:Interleukin-24; C494086:STAT3 protein, human; C492510:interleukin-20 receptor