title: Nanotoxicological Effects of SiO2 Nanoparticles on Spodoptera frugiperda Sf9 Cells.
abstract: The application of silica nanoparticles (NPs) in the biomedical field experienced a great development. The driving forces for these and future developments are the possibility to design NPs with homogeneous size and structure amenable to specific grafting. Moreover, it is possible to tune the characteristics of the NPs to meet the requirements of each specific cell and desired application. Herein, we analyzed the effect of silica NPs of various sizes and surface charge on the viability of Spodoptera frugiperda cells (Sf9 cell line) with the aim of extending the knowledge of possible toxicity of the NPs in the environment and development of new tools for insect control. Moreover, these results will also contribute to develop more effective systems for gene vectors delivery and recombinant proteins expression. Bare silica NPs of 14 nm, 380 nm and 1430 nm as well as amine-modified silica NPs of 131 nm and 448 nm were obtained by the Stöber method. The NPs were characterized by DLS and zeta potential measurements. The cell viability was assessed by the MTT test. It was observed that the 14 nm NPs possess the highest toxic effect. Indeed, after 24 h, the viability of the cells exposed to the lower concentration of NPs (0.12 mg/ml) was about 40% of the value obtained for the control cells not exposed to NPs. Moreover, the exposure to other negative charged NPs also causes a lower activity when compared with the control. Alternatively, lower concentrations of positive charged NPs (i.e.: 0.12 or 0.6 mg/ml) demonstrated to stimulate the proliferation of the cells and higher concentrations (i.e.: 7.2 mg/ml) did not present significant differences with the control. In conclusion, we have demonstrated that the NPs possess an effect that is highly influenced by the size, charge and concentration. Although, silica NPs are being used in the biomedical field, these results contribute to further understanding the risk that could be associated to nanoparticles and how these can be modified in order to meet the requirements of each desired application.
authors.affiliation: Centro de Investigaciones en Plagas e Insecticidas (CIPEIN), Instituto de Investigaciones Científicas y Tecnicas para la Defensa CITEDEF/UNIDEF, Consejo Nacional de Investigaciones Científicas y Tecnicas, Buenos Aires, Argentina (CONICET). Juan B. De La Salle 4397 (B1603ALO), Villa Martelli, Buenos Aires, Argentina. psantoorihuela@conicet.gov.ar.
authors.forename: Pablo L
authors.initials: PL
authors.lastname: Santo-Orihuela
authors.forename: Maria L
authors.initials: ML
authors.lastname: Foglia
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Targovnik
authors.forename: Maria V
authors.initials: MV
authors.lastname: Miranda
authors.forename: Martin F
authors.initials: MF
authors.lastname: Desimone
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry*; D061987:Sf9 Cells; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*; D018411:Spodoptera
chemical_list: D012822:Silicon Dioxide