title: Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal.
abstract: The porcine major histocompatibility complex (MHC) is called swine leukocyte antigen (SLA), which controls immune responses and transplantation reactions. The SLA is mapped on pig chromosome 7 (SSC7) near the centromere. In this study, 3 class I (SLA-1, SLA-3, and SLA-2) and 3 class II (DRB1, DQB1, and DQA) genes were used for investigation of SLA haplotypes in Yucatan miniature pigs in Korea. This pig breed is a well-known model organism for biomedical research worldwide. The current study indicated that Korean Yucatan pig population had 3 Class I haplotypes (Lr-4.0, Lr-6.0, and Lr-25.0) and 3 class II haplotypes (Lr-0.5, Lr-0.7, and Lr-0.25). The combinations of SLA class I and II haplotype together, 2 homozygous (Lr-4.5/4.5 and Lr-6.7/6.7) and 3 heterozygous (Lr-4.5/6.7, Lr-4.5/25.25, and Lr-6.7/25.25) haplotypes were identified, including previously unidentified new heterozygous haplotypes (Lr-4.5/4.7). In addition, a new SLA allele typing method using Agilent 2100 bioanalyzer was developed that permitted more rapid identification of SLA haplotypes. These results will facilitate the breeding of SLA homozygous Yucatan pigs and will expedite the possible use of these pigs for the biomedical research, especially xenotransplantation research.
authors.affiliation: Optipharm Inc, Cheongju 28158, Korea .
authors.forename: Nu-Ri
authors.initials: NR
authors.lastname: Choi
authors.affiliation: Optipharm Inc, Cheongju 28158, Korea .
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Optipharm Inc, Cheongju 28158, Korea .
authors.forename: Ki-Myung
authors.initials: KM
authors.lastname: Choi
authors.affiliation: Optipharm Inc, Cheongju 28158, Korea .
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Ko
authors.affiliation: Optipharm Inc, Cheongju 28158, Korea .
authors.forename: Ji-Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Optipharm Inc, Cheongju 28158, Korea .
authors.forename: Hyun-Il
authors.initials: HI
authors.lastname: Kim
authors.affiliation: Hanwoo Improvement Center, National Agricultural Cooperative Federation, Seosan 31948, Korea .
authors.forename: Woo-Young
authors.initials: WY
authors.lastname: Jung
authors.affiliation: Optipharm Inc, Cheongju 28158, Korea .
authors.forename: Jun-Heon
authors.initials: JH
authors.lastname: Lee
keywords: Haplotype; Major Histocompatibility Complex [MHC]; Swine Leukocyte Antigen [SLA]; Yucatan Miniature Pig