title: [Histologic detection of cytomegalovirus and its application in gastrointestinal cytomegalovirus infection].
abstract: OBJECTIVE
  To investigate the clinical and pathologic features of gastrointestinal cytomegalovirus (CMV) disease, and the histological detection of CMV.
  
  
  METHODS
  Forty-one gastrointestinal tissues were obtained from 35 patients suspected for gastrointestinal CMV disease. The pathologic changes of these specimens were evaluated by immunohistochemistry and in situ hybridization for CMV, and these were compared to serologic detection methods.
  
  
  RESULTS
  CMV was detected in 23/41 tissues. There were 32 serologic CMV DNA tests at the time of biopsies or operations. Compared with histological detection, the sensitivity and specificity of serologic CMV DNA tests were 73.7% and 69.2% respectively.
  
  
  CONCLUSIONS
  Gastrointestinal histological detection of CMV has important clinical application. HE staining combined with immunohistochemistry and/or in situ hybridization detection is a reliable method to detect CMV.
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
authors.forename: Weixun
authors.initials: W
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Qing
authors.initials: Q
authors.lastname: Ling
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Luo
mesh_terms: D001706:Biopsy; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D005767:Gastrointestinal Diseases / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization