title: Viral protein requirements for assembly and release of human parainfluenza virus type 3 virus-like particles.
abstract: To understand the roles of human parainfluenza virus 3 (HPIV3) proteins in assembly and release, viral proteins were expressed individually and in combination in 293T cells. Expression of the matrix (M) protein triggered release of enveloped, matrix-containing virus-like particles (VLPs) from cells. When M was co-expressed with the nucleocapsid (N), fusion (F) or haemagglutinin-neuraminidase (HN) proteins, VLPs that contained M+N, M+F and M+HN, respectively, were generated, suggesting that M can independently interact with each protein to facilitate assembly and release. Additionally, expression of N protein enabled incorporation of the phosphoprotein (P) into VLPs, likely due to known N-P interactions. Finally, the HPIV3 C protein did not enhance VLP release, in contrast to observations with the related Sendai virus. These findings reinforce the central importance of the M protein in virus assembly and release, but also illustrate the variable roles of other paramyxovirus proteins during these processes.
authors.affiliation: Department of Microbiology, University of Wisconsin - La Crosse, 1725 State St, La Crosse, WI 54601, USA.
authors.forename: Megan K
authors.initials: MK
authors.lastname: Bracken
authors.affiliation: Department of Microbiology, University of Wisconsin - La Crosse, 1725 State St, La Crosse, WI 54601, USA.
authors.forename: Brandon C
authors.initials: BC
authors.lastname: Hayes
authors.affiliation: Department of Microbiology, University of Wisconsin - La Crosse, 1725 State St, La Crosse, WI 54601, USA.
authors.forename: Suresh R
authors.initials: SR
authors.lastname: Kandel
authors.affiliation: Department of Microbiology, University of Wisconsin - La Crosse, 1725 State St, La Crosse, WI 54601, USA.
authors.forename: Deja
authors.initials: D
authors.lastname: Scott-Shemon
authors.affiliation: Department of Microbiology, University of Wisconsin - La Crosse, 1725 State St, La Crosse, WI 54601, USA.
authors.forename: Larissa
authors.initials: L
authors.lastname: Ackerson
authors.affiliation: Department of Microbiology, University of Wisconsin - La Crosse, 1725 State St, La Crosse, WI 54601, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Hoffman
mesh_terms: D002460:Cell Line; D006801:Humans; D010224:Parainfluenza Virus 3, Human / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding; D055503:Protein Multimerization; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D022701:Virosomes / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D057074:Virus Release
chemical_list: D015678:Viral Structural Proteins; D022701:Virosomes