title: Burn injury induces histopathological changes and cell proliferation in liver of rats.
abstract: OBJECTIVE
  To investigate effects of severe burn injury (BI) in rat liver through the histopathological and inflammatory markers analysis.
  
  
  METHODS
  Forty-two male Wistar rats were distributed into two groups, control (C) and subjected to scald BI (SBI). The animals were euthanized one, four and 14 d post sham or 45% of the total body surface BI. Liver fragments were submitted to histopathological, morphoquantitative (hepatocyte area and cell density), ciclooxigenase-2 (COX-2) immunoexpression, and gene expression [real-time polymerase chain reaction for tumor necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS) and caspase-3] methods.
  
  
  RESULTS
  Histopathological findings showed inflammatory process in all periods investigated and hepatocyte degeneration added to increased amount of connective tissue 14 d post injury. Hepatocyte area, the density of binucleated hepatocytes and density of sinusoidal cells of SBI groups were increased when compared with control. COX-2 immunoexpression was stronger in SBI groups. No differences were found in TNF-α, iNOS and caspase-3 gene expression.
  
  
  CONCLUSIONS
  BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats.
authors.affiliation: Jeferson André Bortolin, Hananiah Tardivo Quintana, Tabata de Carvalho Tomé, Flavia Andressa Pidone Ribeiro, Daniel Araki Ribeiro, Flavia de Oliveira, Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP 11060-001, Brazil.
authors.forename: Jeferson André
authors.initials: JA
authors.lastname: Bortolin
authors.affiliation: Jeferson André Bortolin, Hananiah Tardivo Quintana, Tabata de Carvalho Tomé, Flavia Andressa Pidone Ribeiro, Daniel Araki Ribeiro, Flavia de Oliveira, Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP 11060-001, Brazil.
authors.forename: Hananiah Tardivo
authors.initials: HT
authors.lastname: Quintana
authors.affiliation: Jeferson André Bortolin, Hananiah Tardivo Quintana, Tabata de Carvalho Tomé, Flavia Andressa Pidone Ribeiro, Daniel Araki Ribeiro, Flavia de Oliveira, Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP 11060-001, Brazil.
authors.forename: Tabata de Carvalho
authors.initials: Tde C
authors.lastname: Tomé
authors.affiliation: Jeferson André Bortolin, Hananiah Tardivo Quintana, Tabata de Carvalho Tomé, Flavia Andressa Pidone Ribeiro, Daniel Araki Ribeiro, Flavia de Oliveira, Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP 11060-001, Brazil.
authors.forename: Flavia Andressa Pidone
authors.initials: FA
authors.lastname: Ribeiro
authors.affiliation: Jeferson André Bortolin, Hananiah Tardivo Quintana, Tabata de Carvalho Tomé, Flavia Andressa Pidone Ribeiro, Daniel Araki Ribeiro, Flavia de Oliveira, Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP 11060-001, Brazil.
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
authors.affiliation: Jeferson André Bortolin, Hananiah Tardivo Quintana, Tabata de Carvalho Tomé, Flavia Andressa Pidone Ribeiro, Daniel Araki Ribeiro, Flavia de Oliveira, Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP 11060-001, Brazil.
authors.forename: Flavia
authors.initials: F
authors.lastname: de Oliveira
keywords: Burn injury; Ciclooxigenase-2; Histophatology; Liver; Morphology