title: RNA Interference for Functional Genomics and Improvement of Cotton (Gossypium sp.).
abstract: RNA interference (RNAi), is a powerful new technology in the discovery of genetic sequence functions, and has become a valuable tool for functional genomics of cotton (Gossypium sp.). The rapid adoption of RNAi has replaced previous antisense technology. RNAi has aided in the discovery of function and biological roles of many key cotton genes involved in fiber development, fertility and somatic embryogenesis, resistance to important biotic and abiotic stresses, and oil and seed quality improvements as well as the key agronomic traits including yield and maturity. Here, we have comparatively reviewed seminal research efforts in previously used antisense approaches and currently applied breakthrough RNAi studies in cotton, analyzing developed RNAi methodologies, achievements, limitations, and future needs in functional characterizations of cotton genes. We also highlighted needed efforts in the development of RNAi-based cotton cultivars, and their safety and risk assessment, small and large-scale field trials, and commercialization.
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Ibrokhim Y
authors.initials: IY
authors.lastname: Abdurakhmonov
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Mirzakamol S
authors.initials: MS
authors.lastname: Ayubov
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Khurshida A
authors.initials: KA
authors.lastname: Ubaydullaeva
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Zabardast T
authors.initials: ZT
authors.lastname: Buriev
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Shukhrat E
authors.initials: SE
authors.lastname: Shermatov
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Haydarali S
authors.initials: HS
authors.lastname: Ruziboev
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" AssociationKibray, Uzbekistan; Laboratory of Plant Physiology, Wageningen UniversityWageningen, Netherlands.
authors.forename: Umid M
authors.initials: UM
authors.lastname: Shapulatov
authors.affiliation: Crop Science Research Laboratory, United States Department of Agriculture - Agricultural Research Service, Starkville MS, USA.
authors.forename: Sukumar
authors.initials: S
authors.lastname: Saha
authors.affiliation: Plant Stress and Germplasm Development Research, United States Department of Agriculture - Agricultural Research Service, Lubbock TX, USA.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Ulloa
authors.affiliation: Crop Germplasm Research Unit, United States Department of Agriculture - Agricultural Research Service, College Station TX, USA.
authors.forename: John Z
authors.initials: JZ
authors.lastname: Yu
authors.affiliation: Crop Germplasm Research Unit, United States Department of Agriculture - Agricultural Research Service, College Station TX, USA.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Percy
authors.affiliation: Department of Obstetrics and Gynecology, University of Iowa Carver College of Medicine, Iowa City IA, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Devor
authors.affiliation: Department of Biological and Environmental Sciences, Alabama A&M University, Normal AL, USA.
authors.forename: Govind C
authors.initials: GC
authors.lastname: Sharma
authors.affiliation: Department of Biological and Environmental Sciences, Alabama A&M University, Normal AL, USA.
authors.forename: Venkateswara R
authors.initials: VR
authors.lastname: Sripathi
authors.affiliation: Dow AgroSciences LLC, Indianapolis IN, USA.
authors.forename: Siva P
authors.initials: SP
authors.lastname: Kumpatla
authors.affiliation: Laboratory of Plant Physiology, Wageningen University Wageningen, Netherlands.
authors.forename: Alexander
authors.initials: A
authors.lastname: van der Krol
authors.affiliation: Agricultural and Environmental Research, Cary NC, USA.
authors.forename: Hake D
authors.initials: HD
authors.lastname: Kater
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Khakimdjan
authors.initials: K
authors.lastname: Khamidov
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Shavkat I
authors.initials: SI
authors.lastname: Salikhov
authors.affiliation: Crop Science Research Laboratory, United States Department of Agriculture - Agricultural Research Service, Starkville MS, USA.
authors.forename: Johnie N
authors.initials: JN
authors.lastname: Jenkins
authors.affiliation: Center of Genomics and Bioinformatics, Structural and Functional Genomics, Academy of Sciences the Republic of Uzbekistan, Ministry of Agriculture and Water Resources the Republic of Uzbekistan and "Uzpakhtasanoat" Association Kibray, Uzbekistan.
authors.forename: Abdusattor
authors.initials: A
authors.lastname: Abdukarimov
authors.affiliation: Department of Biology, Texas A&M University, Colleges Station TX, USA.
authors.forename: Alan E
authors.initials: AE
authors.lastname: Pepper
keywords: Gossypium; antisense; cotton pest control; disease resistance; fiber quality; gene silencing