title: Some considerations on the current debate about typing resolution in solid organ transplantation.
abstract: BACKGROUND
  The shortage of suitable organs and achieved tolerance are uncontested main concerns in transplantation. Long waiting lists for deceased donors and limited numbers of living donors are the current scenarios. Kidney grafts from living donors have better overall survival compared to cadaveric and require less aggressive immunosuppressive regimens. The human leukocyte antigen (HLA) labs have the key role to test the recipient and donors compatibility based on typing and antibody profile. The current standard molecular procedure in solid organ transplantation is low-resolution typing, at the antigen level.
  
  
  METHODS
  In this commentary, the merits of high versus low degree of typing resolution in solid organ transplantation are discussed. Critical questions and reasons to bring high-resolution typing as a routine test in health system are considered. Specifically, with the introduction of the next-generation sequencing (NGS) in HLA, the pros and cons in living donation and benefits after deceased donation are critically evaluated.
  
  
  CONCLUSIONS
  NGS has the potential to improve the transplant rates and the overall graft survival. Alternative strategies to increase in demanding the number of transplants are briefly highlighted.
authors.affiliation: Department of Pathology, Tissue Typing Laboratory, University of Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 USA.
authors.forename: Paraskevi
authors.initials: P
authors.lastname: Vogiatzi
keywords: Allele-based mismatch; Antigen-based mismatch; Cadaveric donor; HLA typing; Living donor; Next generation sequencing; Solid organ transplantation