title: Cholinergic modulation of auditory steady-state response in the auditory cortex of the freely moving rat.
abstract: As disturbance in auditory steady-state response (ASSR) has been consistently found in many neuropsychiatric disorders, such as autism spectrum disorder and schizophrenia, there is considerable interest in the development of translational rat models to elucidate the underlying neural and neurochemical mechanisms involved in ASSR. This is the first study to investigate the effects of the non-selective muscarinic antagonist scopolamine and the cholinesterase inhibitor donepezil (also in combination with scopolamine) on ASSR. We recorded the local field potentials through the chronic microelectrodes implanted in the auditory cortex of freely moving rat. ASSRs were recorded in response to auditory stimuli delivered over a range of frequencies (10-80 Hz) and averaged over 60 trials. We found that a single dose of scopolamine produced a temporal attenuation in response to auditory stimuli; the most attenuation occurred at 40 Hz. Time-frequency analysis revealed deficits in both power and phase-locking to 40 Hz. Donepezil augmented 40-Hz steady-state power and phase-locking. Scopolamine combined with donepezil had an enhanced effect on the phase-locking, but not power of ASSR. These changes induced by cholinergic drugs suggest an involvement of muscarinic neurotransmission in auditory processing and provide a rodent model investigating the neurochemical mechanism of neurophysiological deficits seen in patients.
authors.affiliation: Department of Physiology, China Medical University, Shenyang 110001, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Physiology, China Medical University, Shenyang 110001, People's Republic of China; Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110001, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Physiology, China Medical University, Shenyang 110001, People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Rheumatology and Immunology, First Affiliated Hospital, China Medical University, Shenyang 110001, People's Republic of China.
authors.forename: P
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Physiology, China Medical University, Shenyang 110001, People's Republic of China; Institute of Pathology and Pathophysiology, China Medical University, Shenyang 110001, People's Republic of China. Electronic address: qinlingling@yahoo.com.
authors.forename: L
authors.initials: L
authors.lastname: Qin
mesh_terms: D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D001303:Auditory Cortex / Q000187:drug effects* / Q000502:physiology*; D018680:Cholinergic Antagonists / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D000077265:Donepezil; D004567:Electrodes, Implanted; D007189:Indans / Q000494:pharmacology*; D008297:Male; D008839:Microelectrodes; D010880:Piperidines / Q000494:pharmacology*; D017208:Rats, Wistar; D012601:Scopolamine / Q000494:pharmacology*
chemical_list: D018680:Cholinergic Antagonists; D002800:Cholinesterase Inhibitors; D007189:Indans; D010880:Piperidines; D000077265:Donepezil; D012601:Scopolamine
keywords: ASSR; auditory cortex; donepezil; local field potential; neurophysiological recording; scopolamine