title: Influence of carboxylic ion-pairing reagents on retention of peptides in thin-layer chromatography systems with C18 silica-based adsorbents.
abstract: One of the main problems related to chromatography of peptides concerns adverse interactions of their strong basic groups with free silanol groups of the silica based stationary phase. Influence of type and concentration of ion-pairing regents on peptide retention in reversed-phase high-performance liquid chromatography (RP-HPLC) systems has been discussed before. Here we present influence of these mobile phase additives on retention of some peptide standards in high-performance thin-layer chromatography (HPTLC) systems with C18 silica-based adsorbents. We prove, that due to different characteristic of adsorbents used in both techniques (RP HPLC and HPTLC), influence of ion-pairing reagents on retention of basic and/or amphoteric compounds also may be quite different. C18 silica-based HPTLC adsorbents provide more complex mechanism of retention and should be rather considered as mixed-mode adsorbents.
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland. Electronic address: radoslaw.gwarda@umlub.pl.
authors.forename: Radosław Ł
authors.initials: RŁ
authors.lastname: Gwarda
authors.affiliation: Chair and Department of Synthesis and Chemical Technology of Pharmaceutical Substances, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Aletańska-Kozak
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Klimek-Turek
authors.affiliation: Chair and Department of Synthesis and Chemical Technology of Pharmaceutical Substances, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziajko-Jankowska
authors.affiliation: Chair and Department of Synthesis and Chemical Technology of Pharmaceutical Substances, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Matosiuk
authors.affiliation: Department of Physical Chemistry, Chair of Chemistry, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 4a Chodźki St., 20-093 Lublin, Poland.
authors.forename: Tadeusz H
authors.initials: TH
authors.lastname: Dzido
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D010455:Peptides; D012822:Silicon Dioxide
keywords: Ion-pairing reagents; Peptide retention; Peptide separation; Reversed-phase chromatography; Thin-layer chromatography