title: Porous SiO2 nanofiber grafted novel bioactive glass-ceramic coating: A structural scaffold for uniform apatite precipitation and oriented cell proliferation on inert implant.
abstract: A composite bioactive glass-ceramic coating grafted with porous silica nanofibers was fabricated on inert glass to provide a structural scaffold favoring uniform apatite precipitation and oriented cell proliferation. The coating surfaces were investigated thoroughly before and after immersion in simulated body fluid. In addition, the proliferation behavior of fibroblast cells on the surface was observed for several culture times. The nanofibrous exterior of this composite bioactive coating facilitated homogeneous growth of flake-like carbonated hydroxyapatite layer within a short period of immersion. Moreover, the embedded porous silica nanofibers enhanced hydrophilicity which is required for proper cell adhesion on the surface. The cells proliferated well following a particular orientation on the entire coating by the assistance of nanofibrous scaffold-like structural matrix. This newly engineered composite coating was effective in creating a biological structural matrix favorable for homogeneous precipitation of calcium phosphate, and organized cell growth on the inert glass surface.
authors.affiliation: Nano-Structured Materials Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata 700032, India.
authors.forename: Indranee
authors.initials: I
authors.lastname: Das
authors.affiliation: Nano-Structured Materials Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata 700032, India. Electronic address: gde@cgcri.res.in.
authors.forename: Goutam
authors.initials: G
authors.lastname: De
authors.affiliation: Johan Gadolin Process Chemistry Centre, Åbo Akademi University, FI-20500 Åbo, Finland.
authors.forename: Leena
authors.initials: L
authors.lastname: Hupa
authors.affiliation: Turku Clinical Biomaterials Centre-TCBC, University of Turku, FI-20520 Turku, Finland; Institute of Dentistry, University of Turku, Department of Biomaterials Science and City of Turku, Welfare Division, Turku, Finland.
authors.forename: Pekka K
authors.initials: PK
authors.lastname: Vallittu
mesh_terms: D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry*; D015921:Dental Implants; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D015921:Dental Implants; D012822:Silicon Dioxide
keywords: Bioactive glass–ceramic; Carbonated hydroxyapatite; Cell proliferation; Coating; Nanofiber; Scaffold