title: Immunoglobulin heavy chain variable region gene repertoire and B-cell receptor stereotypes in Indian patients with chronic lymphocytic leukemia.
abstract: In chronic lymphocytic leukemia (CLL), the geographical bias in immunoglobulin heavy-chain variable (IGHV) gene usage lead us to analyze IGHV gene usage and B-cell receptor stereotypy in 195 patients from India. IGHV3, IGHV4, and IGHV1 families were the most frequently used. 20.5% sequences had stereotyped BCR and were clustered in 12 pre-defined and 6 novel subsets. Unmutated IGHV was significantly associated with reduced time to first treatment (p < 0.033) and poor overall survival (OS; p = 0.01). We observed a significant difference in OS between IGHV1, IGHV3, and IGHV4 family cases (p = 0.045) in early stage patients. Regarding subfamily usage, only IGHV1-69 expression was found to have statistically significant poor outcome (p = 0.017). Our results from the analysis of various molecular and clinical features suggest that the expression of specific IGHV gene influences the outcome in early stage CLL, and hence its assessment may be added to the clinical leukemia laboratory armamentarium.
authors.affiliation: a Laboratory Oncology Unit , Dr. Bhim Rao Ambedkar Institute Rotary Cancer Hospital (Dr. B.R.A.IRCH), All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India ;
authors.forename: Lata
authors.initials: L
authors.lastname: Rani
authors.affiliation: a Laboratory Oncology Unit , Dr. Bhim Rao Ambedkar Institute Rotary Cancer Hospital (Dr. B.R.A.IRCH), All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India ;
authors.forename: Nitin
authors.initials: N
authors.lastname: Mathur
authors.affiliation: b Department of Medical Oncology , Dr. Bhim Rao Ambedkar Institute Rotary Cancer Hospital (Dr. B.R.A.IRCH), All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India ;
authors.forename: Ajay
authors.initials: A
authors.lastname: Gogia
authors.affiliation: c Department of Biostatistics , All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India ;
authors.forename: Sreenivas
authors.initials: S
authors.lastname: Vishnubhatla
authors.affiliation: b Department of Medical Oncology , Dr. Bhim Rao Ambedkar Institute Rotary Cancer Hospital (Dr. B.R.A.IRCH), All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India ;
authors.forename: Lalit
authors.initials: L
authors.lastname: Kumar
authors.affiliation: b Department of Medical Oncology , Dr. Bhim Rao Ambedkar Institute Rotary Cancer Hospital (Dr. B.R.A.IRCH), All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India ;
authors.forename: Atul
authors.initials: A
authors.lastname: Sharma
authors.affiliation: d Department of Biophysics , All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India.
authors.forename: Divya
authors.initials: D
authors.lastname: Dube
authors.affiliation: d Department of Biophysics , All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India.
authors.forename: Punit
authors.initials: P
authors.lastname: Kaur
authors.affiliation: a Laboratory Oncology Unit , Dr. Bhim Rao Ambedkar Institute Rotary Cancer Hospital (Dr. B.R.A.IRCH), All India Institute of Medical Sciences (AIIMS) , Ansari Nagar , New Delhi , India ;
authors.forename: Ritu
authors.initials: R
authors.lastname: Gupta
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D022801:Complementarity Determining Regions / Q000235:genetics; D004252:DNA Mutational Analysis; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D015322:Gene Rearrangement, B-Lymphocyte; D050438:Genes, Immunoglobulin Heavy Chain / Q000235:genetics*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007194:India; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000628:therapy; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D009154:Mutation; D009367:Neoplasm Staging; D011379:Prognosis; D011947:Receptors, Antigen, B-Cell / Q000235:genetics*; D061665:Time-to-Treatment
chemical_list: D022801:Complementarity Determining Regions; D007135:Immunoglobulin Variable Region; D011947:Receptors, Antigen, B-Cell
keywords: B-cell receptor; VH CDR3; immunoglobulin heavy-chain variable genes; stereotyped receptor