title: Co-cultivation of keratinocyte-human mesenchymal stem cell (hMSC) on sericin loaded electrospun nanofibrous composite scaffold (cationic gelatin/hyaluronan/chondroitin sulfate) stimulates epithelial differentiation in hMSCs: In vitro study.
abstract: Fortifying the scaffold with bioactive molecules and glycosaminoglycans (GAGs), is an efficient way to design new generation tissue engineered biomaterials. In this study, we evaluated the synergistic effect of electrospun nanofibrous composite scaffold (cationic gelatin/hyaluronan/chondroitin sulfate) loaded with sericin and, contact co-culture of human mesenchymal stem cells (hMSCs)-keratinocytes on hMSCs' differentiation towards epithelial lineage. Cationic gelatin is prepared with one step novel synthesis process by grafting quaternary ammonium salts to the backbone of gelatin. Release kinetics studies showed that Fickian diffusion is the major release mechanism for both GAGs and sericin/gelatin. In vitro biocompatibility of the electrospun scaffold was evaluated in terms of LDH and DNA quantification assay on human foreskin fibroblast, human keratinocyte and hMSC. Significant proliferation (∼ 4-6 fold) was detected after culturing all three cell on the electrospun scaffold containing sericin. After 5 days of contact co-culture, results revealed that electrospun scaffold containing sericin promote epithelial differentiation of hMSC in terms of several protein markers (keratin 14, ΔNp63α and Pan-cytokeratin) and gene expression of some dermal proteins (keratin 14, ΔNp63α). Findings of this study will foster the progress of current skin tissue engineering scaffolds by understanding the skin regeneration and wound healing process.
authors.affiliation: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, Germany; Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, 110016 New Delhi, India.
authors.forename: Sirsendu
authors.initials: S
authors.lastname: Bhowmick
authors.affiliation: Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Scharnweber
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, 110016 New Delhi, India. Electronic address: veenak@iitd.ac.in.
authors.forename: Veena
authors.initials: V
authors.lastname: Koul
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002809:Chondroitin Sulfates / Q000737:chemistry*; D018920:Coculture Techniques; D005780:Gelatin / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D005780:Gelatin; D002809:Chondroitin Sulfates
keywords: Cellular proliferation and differentiation; Electrospinning; Gelatin; Glycosaminoglycan; Sericin