title: Phylogenetic evidence for intratypic recombinant events in a novel human adenovirus C that causes severe acute respiratory infection in children.
abstract: Human adenoviruses (HAdVs) are prevalent in hospitalized children with severe acute respiratory infection (SARI). Here, we report a unique recombinant HAdV strain (CBJ113) isolated from a HAdV-positive child with SARI. The whole-genome sequence was determined using Sanger sequencing and high-throughput sequencing. A phylogenetic analysis of the complete genome indicated that the CBJ113 strain shares a common origin with HAdV-C2, HAdV-C6, HAdV-C1, HAdV-C5, and HAdV-C57 and formed a novel subclade on the same branch as other HAdV-C subtypes. BootScan and single nucleotide polymorphism analyses showed that the CBJ113 genome has an intra-subtype recombinant structure and comprises gene regions mainly originating from two circulating viral strains: HAdV-1 and HAdV-2. The parental penton base, pVI, and DBP genes of the recombinant strain clustered with the HAdV-1 prototype strain, and the E1B, hexon, fiber, and 100 K genes of the recombinant clustered within the HAdV-2 subtype, meanwhile the E4orf1 and DNA polymerase genes of the recombinant shared the greatest similarity with those of HAdV-5 and HAdV-6, respectively. All of these findings provide insight into our understanding of the dynamics of the complexity of the HAdV-C epidemic. More extensive studies should address the pathogenicity and clinical characteristics of the novel recombinant.
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Roujian
authors.initials: R
authors.lastname: Lu
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Major Diseases in Children and National Key Discipline of Pediatrics Capital Medical University, Ministry of Education, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
authors.forename: Zhengde
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Children Hospital of Fudan University, Shanghai 200032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.affiliation: Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Tan
mesh_terms: D000258:Adenovirus Infections, Human / Q000473:pathology / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D002648:Child; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D006801:Humans; D010802:Phylogeny; D011995:Recombination, Genetic; D012141:Respiratory Tract Infections / Q000473:pathology / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral