title: Intracellular nanoparticles mass quantification by near-edge absorption soft X-ray nanotomography.
abstract: We used soft X-ray three-dimensional imaging to quantify the mass of superparamagnetic iron oxide nanoparticles (SPION) within whole cells, by exploiting the iron oxide differential absorption contrast. Near-edge absorption soft X-ray nanotomography (NEASXT) combines whole-cell 3D structure determination at 50 nm resolution, with 3D elemental mapping and high throughput. We detected three-dimensional distribution of SPIONs within cells with 0.3 g/cm(3) sensitivity, sufficient for detecting the density corresponding to a single nanoparticle.
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain.
authors.forename: Jose Javier
authors.initials: JJ
authors.lastname: Conesa
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Otón
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain.
authors.forename: Michele
authors.initials: M
authors.lastname: Chiappi
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain.
authors.forename: Jose María
authors.initials: JM
authors.lastname: Carazo
authors.affiliation: ALBA Synchrotron Light Source, MISTRAL Beamline - Experiments Division, 08290 Cerdanyola del Vallès, Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Pereiro
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Chichón
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain.|Unidad Asociada CNB-Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), Cantoblanco, 28049 Madrid, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Carrascosa
mesh_terms: D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles; D055114:X-Ray Microtomography / Q000379:methods*