title: First report of the emerging zoonotic agent Wohlfahrtiimonas chitiniclastica isolated from a retail frozen chicken in Rio de Janeiro, Brazil.
abstract: Wohlfahrtiimonas chitiniclastica is an emerging zoonotic bacterium commensally living in larvae of particular flies. It has been associated with human and animal infections but never isolated from food. In the present study, a whole chicken carcass was rinsed in buffered peptone water which was then inoculated into BHI and the growth plated onto selective medium. Species identification was performed by MALDI-TOF MS. Those bacteria identified as W. chitiniclastica were subjected to 16S rRNA sequencing for confirmation and MEGA software was used to obtain their phylogenetic position. The findings of this study raise concerns regarding the abattoir, transport and stock practices of frozen meat carcasses and should be of interest with regard to microbiology, entomology and food production.
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Medical Sciences Faculty, Rio de Janeiro State University, Avenida Professor Manoel de Abreu, 444 - 3°andar, Vila Isabel, Rio de Janeiro, RJ, 20550-170, Brazil. georgecmatos@gmail.com.
authors.forename: Jorge
authors.identifier: http://orcid.org/0000-0002-5641-9690
authors.initials: J
authors.lastname: Matos
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Medical Sciences Faculty, Rio de Janeiro State University, Avenida Professor Manoel de Abreu, 444 - 3°andar, Vila Isabel, Rio de Janeiro, RJ, 20550-170, Brazil.
authors.forename: Amanda Portela
authors.initials: AP
authors.lastname: Queiroga
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Medical Sciences Faculty, Rio de Janeiro State University, Avenida Professor Manoel de Abreu, 444 - 3°andar, Vila Isabel, Rio de Janeiro, RJ, 20550-170, Brazil.
authors.forename: Carlla Cristhina
authors.initials: CC
authors.lastname: de Oliveira Pedroza Bindi dos Reis
authors.affiliation: Medicine Faculty, Paulo de Góes Microbiology Institute, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, 373 - bloco I, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.
authors.forename: Rachel Leite
authors.initials: RL
authors.lastname: Ribeiro
authors.affiliation: Medicine Faculty, Paulo de Góes Microbiology Institute, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, 373 - bloco I, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.
authors.forename: Lucia Martins
authors.initials: LM
authors.lastname: Teixeira
authors.affiliation: Biochemistry Department, IBRAG, Rio de Janeiro State University, Rio de Janeiro, Avenida Professor Manoel de Abreu, 444 - 5° andar, Vila Isabel, Rio de Janeiro, RJ, 20550-170, Brazil.
authors.forename: Rodolpho Mattos
authors.initials: RM
authors.lastname: Albano
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Medical Sciences Faculty, Rio de Janeiro State University, Avenida Professor Manoel de Abreu, 444 - 3°andar, Vila Isabel, Rio de Janeiro, RJ, 20550-170, Brazil.
authors.forename: Angela Corrêa
authors.initials: AC
authors.lastname: de Freitas-Almeida
authors.affiliation: Department of Microbiology, Immunology and Parasitology, Medical Sciences Faculty, Rio de Janeiro State University, Avenida Professor Manoel de Abreu, 444 - 3°andar, Vila Isabel, Rio de Janeiro, RJ, 20550-170, Brazil.
authors.forename: Mara Lucia Penna
authors.initials: ML
authors.lastname: Queiroz
mesh_terms: D000818:Animals; D001938:Brazil; D002645:Chickens / Q000382:microbiology*; D021821:Communicable Diseases, Emerging / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004175:Diptera / Q000382:microbiology; D005511:Food Handling; D005516:Food Microbiology; D005628:Frozen Foods / Q000382:microbiology*; D008460:Meat / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014946:Wound Infection / Q000382:microbiology; D044166:Xanthomonadaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015047:Zoonoses / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Chicken carcass; Food processing; MALDI–TOF MS; Phylogenetic; Wohlfahrtiimonas chitiniclastica