title: Insight on Genes Affecting Tuber Development in Potato upon Potato spindle tuber viroid (PSTVd) Infection.
abstract: Potato (Solanum tuberosum L) is a natural host of Potato spindle tuber viroid (PSTVd) which can cause characteristic symptoms on developing plants including stunting phenotype and distortion of leaves and tubers. PSTVd is the type species of the family Pospiviroidae, and can replicate in the nucleus and move systemically throughout the plant. It is not well understood how the viroid can affect host genes for successful invasion and which genes show altered expression levels upon infection. Our primary focus in this study is the identification of genes which can affect tuber formation since viroid infection can strongly influence tuber development and especially tuber shape. In this study, we used a large-scale method to identify differentially expressed genes in potato. We have identified defence, stress and sugar metabolism related genes having altered expression levels upon infection. Additionally, hormone pathway related genes showed significant up- or down-regulation. DWARF1/DIMINUTO, Gibberellin 7-oxidase and BEL5 transcripts were identified and validated showing differential expression in viroid infected tissues. Our study suggests that gibberellin and brassinosteroid pathways have a possible role in tuber development upon PSTVd infection.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Crete, Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Katsarou
authors.affiliation: Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Information and Computational Sciences, The James Hutton Institute, Invergowrie, Dundee, United Kingdom.
authors.forename: Runxuan
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, United Kingdom.
authors.forename: Nicola
authors.initials: N
authors.lastname: Bonar
authors.affiliation: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, United Kingdom.
authors.forename: Jenny
authors.initials: J
authors.lastname: Morris
authors.affiliation: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, United Kingdom.
authors.forename: Pete E
authors.initials: PE
authors.lastname: Hedley
authors.affiliation: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, United Kingdom.
authors.forename: Glenn J
authors.initials: GJ
authors.lastname: Bryan
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Crete, Greece.|Department of Biology, University of Crete, Heraklion, Crete, Greece.
authors.forename: Kriton
authors.initials: K
authors.lastname: Kalantidis
authors.affiliation: Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, United Kingdom.
authors.forename: Csaba
authors.initials: C
authors.lastname: Hornyik
mesh_terms: D060406:Brassinosteroids / Q000096:biosynthesis; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D005875:Gibberellins / Q000096:biosynthesis; D054884:Host-Pathogen Interactions; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D010937:Plant Growth Regulators / Q000096:biosynthesis; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000821:virology; D035281:Plant Tubers / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D010942:Plant Viruses / Q000472:pathogenicity / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D014772:Viroids / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D060406:Brassinosteroids; D005875:Gibberellins; D008565:Membrane Proteins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D006899:Mixed Function Oxygenases; C106552:gibberellin 7-oxidase