title: RNA sequencing reveals region-specific molecular mechanisms associated with epileptogenesis in a model of classical hippocampal sclerosis.
abstract: We report here the first complete transcriptome analysis of the dorsal (dDG) and ventral dentate gyrus (vDG) of a rat epilepsy model presenting a hippocampal lesion with a strict resemblance to classical hippocampal sclerosis (HS). We collected the dDG and vDG by laser microdissection 15 days after electrical stimulation and performed high-throughput RNA-sequencing. There were many differentially regulated genes, some of which were specific to either of the two sub-regions in stimulated animals. Gene ontology analysis indicated an enrichment of inflammation-related processes in both sub-regions and of axonal guidance and calcium signaling processes exclusively in the vDG. There was also a differential regulation of genes encoding molecules involved in synaptic function, neural electrical activity and neuropeptides in stimulated rats. The data presented here suggests, in the time point analyzed, a remarkable interaction among several molecular components which takes place in the damaged hippocampi. Furthermore, even though similar mechanisms may function in different regions of the DG, the molecular components involved seem to be region specific.
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL.
authors.forename: A S
authors.initials: AS
authors.lastname: Vieira
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL.
authors.forename: A H
authors.initials: AH
authors.lastname: de Matos
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL.
authors.forename: A M
authors.initials: AM
authors.lastname: do Canto
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL.
authors.forename: C S
authors.initials: CS
authors.lastname: Rocha
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL.
authors.forename: B S
authors.initials: BS
authors.lastname: Carvalho
authors.affiliation: Faculty of Basic Sciences, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil UFF.
authors.forename: V D B
authors.initials: VD
authors.lastname: Pascoal
authors.affiliation: Department of Neurology, Epilepsy Center Hessen, Philipps-University Marburg, Marburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Norwood
authors.affiliation: Department of Neurology, Epilepsy Center Hessen, Philipps-University Marburg, Marburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Bauer
authors.affiliation: Department of Neurology, Epilepsy Center Hessen, Philipps-University Marburg, Marburg, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Rosenow
authors.affiliation: Laboratory of Animal Quality Control (CEMIB), Rua 05 de junho, s/n. Cidade Universitária "Zeferino Vaz". Distrito de Barão Geraldo, Campinas, SP, 13083-877, BRAZIL.
authors.forename: R
authors.initials: R
authors.lastname: Gilioli
authors.affiliation: Department of Neurology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Cendes
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, University of Campinas - UNICAMP, SP, BRAZIL.
authors.forename: I
authors.initials: I
authors.lastname: Lopes-Cendes
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000378:metabolism* / Q000473:pathology; D004827:Epilepsy / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014402:Tuberous Sclerosis / Q000378:metabolism* / Q000473:pathology