title: Parathyroid hormone attenuates radiation-induced increases in collagen crosslink ratio at periosteal surfaces of mouse tibia.
abstract: As part of our ongoing efforts to understand underlying mechanisms contributing to radiation-associated bone fragility and to identify possible treatments, we evaluated the longitudinal effects of parathyroid hormone (PTH) treatment on bone quality in a murine model of limited field irradiation. We hypothesized PTH would mitigate radiation-induced changes in the chemical composition and structure of bone, as measured by microscope-based Raman spectroscopy. We further hypothesized that collagen crosslinking would be especially responsive to PTH treatment. Raman spectroscopy was performed on retrieved tibiae (6-7/group/time point) to quantify metrics associated with bone quality, including: mineral-to-matrix ratio, carbonate-to-phosphate ratio, mineral crystallinity, collagen crosslink (trivalent:divalent) ratio, and the mineral and matrix depolarization ratios. Irradiation disrupted the molecular structure and orientation of bone collagen, as evidenced by a higher collagen crosslink ratio and lower matrix depolarization ratio (vs. non-irradiated control bones), persisting until 12weeks post-irradiation. Radiation transiently affected the mineral phase, as evidenced by increased mineral crystallinity and mineral-to-matrix ratio at 4weeks compared to controls. Radiation decreased bone mineral depolarization ratios through 12weeks, indicating increased mineral alignment. PTH treatment partially attenuated radiation-induced increases in collagen crosslink ratio, but did not restore collagen or mineral alignment. These post-radiation matrix changes are consistent with our previous studies of radiation damage to bone, and suggest that the initial radiation damage to bone matrix has extensive effects on the quality of tissue deposited thereafter. In addition to maintaining bone quality, preventing initial radiation damage to the bone matrix (i.e. crosslink ratio, matrix orientation) may be critical to preventing late-onset fragility fractures.
authors.affiliation: Department of Orthopedic Surgery, Upstate Medical University, Syracuse, NY 13210, USA.
authors.forename: Megan E
authors.initials: ME
authors.lastname: Oest
authors.affiliation: Department of Chemistry; University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Gong
authors.affiliation: Department of Internal Medicine-Rheumatology, University of Michigan Medical School, Ann Arbor, MI 48109.
authors.forename: Karen
authors.initials: K
authors.lastname: Esmonde-White
authors.affiliation: Department of Orthopedic Surgery, Upstate Medical University, Syracuse, NY 13210, USA.
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Mann
authors.affiliation: Department of Orthopedic Surgery, Upstate Medical University, Syracuse, NY 13210, USA.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Zimmerman
authors.affiliation: Department of Orthopedic Surgery, Upstate Medical University, Syracuse, NY 13210, USA.
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Damron
authors.affiliation: Department of Chemistry; University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Morris
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000187:drug effects / Q000528:radiation effects; D002113:Calcification, Physiologic / Q000187:drug effects / Q000528:radiation effects; D003094:Collagen / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000378:metabolism*; D005260:Female; D008807:Mice, Inbred BALB C; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010521:Periosteum / Q000187:drug effects* / Q000528:radiation effects*; D013059:Spectrum Analysis, Raman; D013977:Tibia / Q000187:drug effects* / Q000528:radiation effects*; D014965:X-Rays
chemical_list: D003432:Cross-Linking Reagents; D010281:Parathyroid Hormone; D003094:Collagen
keywords: Bone; Collagen crosslinking; Extracellular matrix; Parathyroid hormone; Radiation therapy; Raman spectroscopy