title: βlinc1 encodes a long noncoding RNA that regulates islet β-cell formation and function.
abstract: Pancreatic β cells are responsible for maintaining glucose homeostasis; their absence or malfunction results in diabetes mellitus. Although there is evidence that long noncoding RNAs (lncRNAs) play important roles in development and disease, none have been investigated in vivo in the context of pancreas development. In this study, we demonstrate that βlinc1 (β-cell long intergenic noncoding RNA 1), a conserved lncRNA, is necessary for the specification and function of insulin-producing β cells through the coordinated regulation of a number of islet-specific transcription factors located in the genomic vicinity of βlinc1. Furthermore, deletion of βlinc1 results in defective islet development and disruption of glucose homeostasis in adult mice.
authors.affiliation: Department of Genetics and Development, Columbia University, New York, New York 10032, USA;
authors.forename: Luis
authors.initials: L
authors.lastname: Arnes
authors.affiliation: Genomic Programming of Β-Cells Laboratory, Institut d'Investigacions August Pi I Sunyer (IDIBAPS), Barcelona 08036, Spain; Department of Medicine, Imperial College London, London W12 0NN, United Kingdom.
authors.forename: Ildem
authors.initials: I
authors.lastname: Akerman
authors.affiliation: Department of Genetics and Development, Columbia University, New York, New York 10032, USA;
authors.forename: Dina A
authors.initials: DA
authors.lastname: Balderes
authors.affiliation: Genomic Programming of Β-Cells Laboratory, Institut d'Investigacions August Pi I Sunyer (IDIBAPS), Barcelona 08036, Spain; Department of Medicine, Imperial College London, London W12 0NN, United Kingdom.
authors.forename: Jorge
authors.identifier: http://orcid.org/0000-0002-5959-5729
authors.initials: J
authors.lastname: Ferrer
authors.affiliation: Department of Genetics and Development, Columbia University, New York, New York 10032, USA;
authors.forename: Lori
authors.initials: L
authors.lastname: Sussel
mesh_terms: D000818:Animals; D002460:Cell Line; D004703:Endocrine System / Q000166:cytology / Q000196:embryology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D055786:Gene Knockout Techniques; D018149:Glucose Intolerance / Q000235:genetics; D006801:Humans; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D062085:RNA, Long Noncoding; D014157:Transcription Factors; C000609068:betalinc1 long noncoding RNA, mouse
keywords: long noncoding RNA; pancreas development; β cell