title: Function of Deubiquitinating Enzyme USP14 as Oncogene in Different Types of Cancer.
abstract: OBJECTIVE
  Non-small cell lung cancer (NSCLC) tissues overexpress USP14, which promotes tumor cell proliferation and is associated with shorter overall survival time.
  
  
  METHODS
  The expression of USP14 was assayed in many types of cancers. USP14 was up-and down-regulated using appropriate plasmid or lentiviral vector constructs and its effects on proliferation, cell colony number, and apoptosis rate were measured. A human NSCLC cell line was inoculated into nude mice and the survival rates were recorded.
  
  
  RESULTS
  We found USP14 amplification and overexpression in many different cancers. The overexpression of USP14 in USP14 low-expression cell lines promoted cell proliferation and migration, whereas USP14 downregulation suppressed tumor cell proliferation, decreased tumor cell colony number, increased apoptosis rate, and decreased cell migration and invasion.
  
  
  CONCLUSIONS
  USP14 plays an oncogenic role in various types of cancer, and may thus represent a new cancer therapy target.
authors.affiliation: Department of Respiratory Medicine, Changhai Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Chaoxiong
authors.initials: C
authors.lastname: Zhang
authors.forename: Changxin
authors.initials: C
authors.lastname: Gu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D016019:Survival Analysis; D043222:Ubiquitin Thiolesterase / Q000235:genetics* / Q000378:metabolism*
chemical_list: C509299:USP14 protein, human; D043222:Ubiquitin Thiolesterase