title: Microfluidic generation of magnetic-fluorescent Janus microparticles for biomolecular detection.
abstract: Fluorescent magnetic multifunctional microparticles were fabricated by a facile droplet microfluidic strategy. Two sodium alginate streams, one doped with Fe3O4 nanoparticles (NPs) and the other with CdSe/ZnS quantum dots, were introduced into a flow-focusing channel as a type of parallel laminar flow to form droplets containing two distinct parts. Then, at the serpentine channel, the Ca(2+) in the oil phase diffused into the droplets, causing the solidification of the droplets. Thus, the Janus microparticles with excellent magnetic/fluorescent properties formed. The flow conditions were optimized and the effects of the flow rates on magnetic/fluorescent compositions were carefully investigated. Luminescent labeling and magnetic separation were simultaneously realized with the newly designed microparticles. Moreover, spatial separation between Fe3O4 NPs and QDs prevented the interference of QDs photoluminescence by the magnetic particles. The as-prepared fluorescent magnetic Janus particles were also successfully employed for DNA assay, which demonstrated the potential of the multifunctional microbeads in biological applications.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Lan
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Jinyang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Ningxing
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xinghu
authors.initials: X
authors.lastname: Ji
authors.affiliation: Department of Clinical Laboratory & Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, PR China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Yu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China; Suzhou Institute of Wuhan University, Suzhou 215123, PR China. Electronic address: zhkhe@whu.edu.cn.
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
mesh_terms: D000464:Alginates / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008856:Microscopy, Fluorescence; D008863:Microspheres; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D045663:Quantum Dots; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D000464:Alginates; D005456:Fluorescent Dyes; D006603:Hexuronic Acids; D058185:Magnetite Nanoparticles; D009841:Oligonucleotides; D020723:Glucuronic Acid; D004247:DNA
keywords: Biomolecular detection; Fluorescence; Janus microparticles; Magnetism; Microfluidics