title: Differences in the ability of two marine annelid species, Thalassodrilides sp. and Perinereis nuntia, to detoxify 1-nitronaphthalene.
abstract: Bioremediation is a promising method for remediating environmentally polluted water. We investigated the abilities of two benthic annelid species to biotransform 1-nitronaphthalene, a nitrated polycyclic aromatic hydrocarbon. We used an oligochaete, Thalassodrilides sp. (Naididae), collected from the sediment beneath a fish farm and a polychaete, Perinereis nuntia, which was obtained from a commercial source. Populations of both organisms were exposed to 1400 μg L(-1) of 1-nitronaphthalene in seawater for 3 days in the dark at 20 °C. The concentration of the pollutant decreased to 12 μg L(-1) in the seawater containing the Thalassodrilides sp. and to 560 μg L(-1) in the seawater containing P. nuntia. The 1-nitronaphthalene concentration in the bodies of the animals increased from 12 to 94 μg kg(-1) in Thalassodrilides sp. and from 0.90 μg kg(-1) to 38,000 μg kg(-1) in P. nuntia. After 3 days, 99% and 40% of the 1-nitronaphthalene had been biotransformed in the Thalassodrilides sp. and P. nuntia experimental groups, respectively. We then tested the acute toxicity of residual 1-nitronaphthalene from the same water using mummichog (fish) larvae. After the larvae had been exposed for 96 h, the percentage of apparently unaffected larvae remaining was 83.3% in Thalassodrilides sp. group but only 16.7% in the P. nuntia group. Clearly, of the two species we studied, Thalassodrilides sp. had a superior ability to convert 1-nitronaphthalene into substances that were nontoxic to mummichog larvae. Therefore, we recommend the use of this species for bioremediation of chemically polluted sediments.
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan. Electronic address: katsuit@affrc.go.jp.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ito
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Mana
authors.initials: M
authors.lastname: Ito
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Onduka
authors.affiliation: South Ehime Fisheries Research Center, Ehime University, 1289-1, Funakoshi, Ainan, Ehime 798-4292, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Ohta
authors.affiliation: IDEA Consultants Inc., 1334-5, Riemon, Yaizu-shi, Shizuoka 421-0212, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Torii
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hano
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Mochida
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Ohkubo
authors.affiliation: South Ehime Fisheries Research Center, Ehime University, 1289-1, Funakoshi, Ainan, Ehime 798-4292, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miura
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, 2-17-5, Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Fujii
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D001711:Biotransformation; D023781:Fundulidae / Q000254:growth & development; D019015:Geologic Sediments / Q000737:chemistry; D007564:Japan; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D009281:Naphthalenes / Q000032:analysis* / Q000378:metabolism; D009835:Oligochaeta / Q000378:metabolism*; D011077:Polychaeta / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
chemical_list: D009281:Naphthalenes; D014874:Water Pollutants, Chemical; C016614:1-nitronaphthalene
keywords: Bioremediation; Marine oligochaete; Nitrated polycyclic aromatic hydrocarbon; Polychaete