title: Insecticidal activity of a novel fatty acid amide derivative from Streptomyces species against Helicoverpa armigera.
abstract: Helicoverpa armigera, an important pest causes serious damage to grain legumes. The main objective of this study was to isolate and identify the metabolite against H. armigera from a previously characterised Streptomyces sp. CAI-155. The culture filtrate of CAI-155 was extracted using Diaion HP-20 and the active fractions were fractionated on Silica and C18 column chromatography. The C18 active fraction was further fractionated on Silica gel 60 F254thin layer chromatography (TLC). The most active fraction (Rf 0.64) purified from TLC led to the identification of a novel metabolite N-(1-(2,2-dimethyl-5-undecyl-1,3-dioxolan-4-yl)-2-hydroxyethyl)stearamide by spectral studies. The purified metabolite showed 70-78% mortality in 2nd instar H. armigera by diet impregnation assay, detached leaf assay and greenhouse assay. The LD50and LD90values of the purified metabolite were 627 and 2276 ppm, respectively. Hence, this novel metabolite can be exploited for pest management in future.
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) , Patancheru , India.
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Gopalakrishnan
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) , Patancheru , India.
authors.forename: Vijayabharathi
authors.initials: V
authors.lastname: Rajendran
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) , Patancheru , India.
authors.forename: Sathya
authors.initials: S
authors.lastname: Arumugam
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) , Patancheru , India.
authors.forename: Hari C
authors.initials: HC
authors.lastname: Sharma
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) , Patancheru , India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Vadlamudi
authors.affiliation: a International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) , Patancheru , India.
authors.forename: Ratna Kumari
authors.initials: RK
authors.lastname: Bhimineni
authors.affiliation: b Department of Chemistry , Norwegian University of Science and Technology (NTNU) , Trondheim , Norway.
authors.forename: Susana
authors.initials: S
authors.lastname: V Gonzalez
authors.affiliation: c Department of Biotechnology , Norwegian University of Science and Technology (NTNU) , Trondheim , Norway.
authors.forename: Torunn
authors.initials: T
authors.lastname: M Melø
authors.affiliation: b Department of Chemistry , Norwegian University of Science and Technology (NTNU) , Trondheim , Norway.
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Simic
keywords: biological control; pest management