title: Age-Associated Resident Memory CD8 T Cells in the Central Nervous System Are Primed To Potentiate Inflammation after Ischemic Brain Injury.
abstract: Aging is associated with an increase in basal inflammation in the CNS and an overall decline in cognitive function and poorer recovery following injury. Growing evidence suggests that leukocyte recruitment to the CNS is also increased with normal aging, but, to date, no systematic evaluation of these age-associated leukocytes has been performed. In this work, the effect of aging on CNS leukocyte recruitment was examined. Aging was associated with more CD45(high) leukocytes, primarily composed of conventional CD8(+) T cells. These results were strain independent and seen in both sexes. Intravascular labeling and immunohistology revealed the presence of parenchymal CD8(+) T cells in several regions of the brain, including the choroid plexus and meninges. These cells had effector memory (CD44(+)CD62L(-)) and tissue-resident phenotypes and expressed markers associated with TCR activation. Analysis of TCRvβ repertoire usage suggested that entry into the CNS is most likely stochastic rather than Ag driven. Correlational analyses revealed a positive association between CD8 T cell numbers and decreased proinflammatory function of microglia. However, the effects of cerebral ischemia and ex vivo stimulation of these cells dramatically increased production of TNF, IFN-γ, and MCP-1/CCL2. Taken together, we identified a novel population of resident memory, immunosurveillant CD8 T cells that represent a hallmark of CNS aging and appear to modify microglia homeostasis under normal conditions, but are primed to potentiate inflammation and leukocyte recruitment following ischemic injury.
authors.affiliation: Neuroscience Department, University of Connecticut Health Center, Farmington, CT 06030;
authors.forename: Rodney M
authors.initials: RM
authors.lastname: Ritzel
authors.affiliation: Neuroscience Department, University of Connecticut Health Center, Farmington, CT 06030;
authors.forename: Joshua
authors.initials: J
authors.lastname: Crapser
authors.affiliation: Neuroscience Department, University of Connecticut Health Center, Farmington, CT 06030;
authors.forename: Anita R
authors.initials: AR
authors.lastname: Patel
authors.affiliation: Neuroscience Department, University of Connecticut Health Center, Farmington, CT 06030;
authors.forename: Rajkumer
authors.initials: R
authors.lastname: Verma
authors.affiliation: Immunology Department, University of Connecticut Health Center, Farmington, CT 06030; and.
authors.forename: Jeremy M
authors.initials: JM
authors.lastname: Grenier
authors.affiliation: Neuroscience Department, University of Connecticut Health Center, Farmington, CT 06030;
authors.forename: Anjali
authors.initials: A
authors.lastname: Chauhan
authors.affiliation: Immunology Department, University of Connecticut Health Center, Farmington, CT 06030; and.
authors.forename: Evan R
authors.initials: ER
authors.lastname: Jellison
authors.affiliation: Neuroscience Department, University of Connecticut Health Center, Farmington, CT 06030; Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77370 Louise.D.McCullough@uth.tmc.edu.
authors.forename: Louise D
authors.initials: LD
authors.lastname: McCullough
mesh_terms: D000367:Age Factors; D000375:Aging / Q000276:immunology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000276:immunology* / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018932:Chemokine CCL2 / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D018960:Hyaluronan Receptors / Q000378:metabolism; D007156:Immunologic Memory / Q000276:immunology; D007157:Immunologic Surveillance / Q000276:immunology; D020244:Infarction, Middle Cerebral Artery / Q000276:immunology* / Q000473:pathology; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D019041:L-Selectin / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D020521:Stroke / Q000276:immunology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D015415:Biomarkers; C491768:Ccl2 protein, mouse; C476399:Cd44 protein, mouse; D018932:Chemokine CCL2; D018960:Hyaluronan Receptors; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014409:Tumor Necrosis Factor-alpha; D019041:L-Selectin; D007371:Interferon-gamma