title: Plexin-B1 indirectly affects glioma invasiveness and angiogenesis by regulating the RhoA/αvβ3 signaling pathway and SRPK1.
abstract: Gliomas are one of the most common primary brain tumors in adults. They display aggressive invasiveness, are highly vascular, and have a poor prognosis. Plexin-B1 is involved in numerous cellular processes, especially cellular migration and angiogenesis. However, the role and regulatory mechanisms of Plexin-B1 in gliomas are not understood and were thus investigated in this study. By using multiple and diverse experimental techniques, we investigated cell apoptosis, mitochondrial membrane potential, cell migration and invasion, angiogenesis, PI3K and Akt phosphorylation, and also the levels of SRPK1 and αvβ3 in glioma cells and animal glioma tissues. The results indicated that Plexin-B1 expression in glioma cell lines is increased compared to normal human astrocytes. Plexin-B1 mediates RhoA/integrin αvβ3 involved in the PI3K/Akt pathway and SRPK1 to influence the growth of glioma cell, angiogenesis, and motility in vitro and in vivo. Thus, Plexin-B1 signaling regulates the Rho/αvβ3/PI3K/Akt pathway and SRPK1, which are involved in glioma invasiveness and angiogenesis. Therefore, the new drug research should focus on Plexin-B1 as a target for the treatment of glioma invasion and angiogenesis.
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.|Qingdao Economic and Technological Development Area First People's Hospital, Qingdao, China.
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Xuyan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Zhuoying
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Junchen
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Feng
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China.
authors.forename: Fengchan
authors.initials: F
authors.lastname: Han
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Southern Medical University, 1023 Shatainan Road, Baiyun District, Guangzhou, 510515, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Huang
authors.affiliation: Neurosurgery, Binzhou Medical University Yantai Affiliated Hospital, 629 Nanhua Road, Muping Area, Shandong Province, 264003, China. yangjun6778@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Human Anatomy and Histology and Embryology, Otology & Neuroscience Center, Binzhou Medical University, 346 Guanhai Road, Laishan District, Shandong Province, 264003, China. hfei22518@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005910:Glioma / Q000473:pathology*; D064593:Heterografts; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D020287:In Situ Nick-End Labeling; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000473:pathology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D039302:Integrin alphaVbeta3; D009419:Nerve Tissue Proteins; C492119:PLXNB1 protein, human; D011956:Receptors, Cell Surface; C087728:SRPK1 protein, human; D017346:Protein Serine-Threonine Kinases; D020742:rhoA GTP-Binding Protein
keywords: Glioma; Plexin-B1; RhoA; SRPK1; αvβ3