title: The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer.
abstract: Breast cancer is the most prevalent type of cancer among women. CD59, a membrane complement regulatory protein, has been demonstrated to be overexpressed in most solid tumors, where it facilitates tumor cell escape from complement surveillance. However, the role of CD59 in breast cancer growth and clinical prognosis is not fully revealed. To investigate the role of CD59 in breast cancer growth and prognostic significance, we knocked down CD59 in a breast cancer cell line that is highly metastatic to the lungs, MDA-MB‑231-HM. Cell growth was measured in vitro and in vivo using a xenograft model. In addition, clinical data on a cohort of 120 patients with or without lung metastasis was analyzed based on CD59 expression, which was detected by immunohistochemistry. Knockdown of CD59 significantly inhibited MDA-MB‑231-HM cell growth both in vitro and in vivo. An analysis of clinical data on 120 patients revealed that patients with CD59 overexpression may have a worse prognosis. CD59 may therefore be a prognostic biomarker for poor outcome in breast cancer patients.
authors.affiliation: Department of Breast Surgery, The Third Hospital of Nanchang, China Jiangxi Province Key Laboratory for Breast Diseases, Nanchang, Jiangxi, P.R. China.
authors.forename: Qianwen
authors.initials: Q
authors.lastname: Ouyang
authors.affiliation: Fudan University Cancer Institute, Shanghai Cancer Center, Shanghai, P.R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Shanghai Cancer Center, Fudan University, Shanghai, P.R. China.
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Shanghai Cancer Center, Fudan University, Shanghai, P.R. China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Ni
authors.affiliation: Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Shanghai Cancer Center, Fudan University, Shanghai, P.R. China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Affiliated Union Hospital of Fujian Medical University, Union Clinical School, Fujian Medical University, Fujian, P.R. China.
authors.forename: Fugui
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, P.R. China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Shanghai Cancer Center, Fudan University, Shanghai, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Fudan University Cancer Institute, Shanghai Cancer Center, Shanghai, P.R. China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Ding
authors.affiliation: Fudan University Cancer Institute, Shanghai Cancer Center, Shanghai, P.R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, P.R. China.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Huang
authors.affiliation: Fudan University Cancer Institute, Shanghai Cancer Center, Shanghai, P.R. China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Shanghai Cancer Center, Fudan University, Shanghai, P.R. China.
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Breast Surgery, The Third Hospital of Nanchang, China Jiangxi Province Key Laboratory for Breast Diseases, Nanchang, Jiangxi, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Fudan University Cancer Institute, Shanghai Cancer Center, Shanghai, P.R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Hu
authors.affiliation: Cancer Center, Traditional Chinese Medicine-Integrated Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Luo
authors.affiliation: Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Shanghai Cancer Center, Fudan University, Shanghai, P.R. China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000328:Adult; D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018957:CD59 Antigens / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000556:secondary*; D051379:Mice; D008875:Middle Aged; D009368:Neoplasm Transplantation; D011379:Prognosis; D015854:Up-Regulation
chemical_list: D018957:CD59 Antigens; C051590:CD59 protein, human