title: Pulsed electromagnetic field (PEMF) prevents pro-oxidant effects of H2O2 in SK-N-BE(2) human neuroblastoma cells.
abstract: Purpose The redox milieu, together with reactive oxygen species (ROS) accumulation, may play a role in mediating some biological effects of extremely-low-frequency electromagnetic fields (ELF-EMF). Some of us have recently reported that a pulsed EMF (PEMF) improves the antioxidant response of a drug-sensitive human neuroblastoma SH-SY5Y cell line to pro-oxidants. Since drug resistance may affect cell sensitivity to redox-based treatments, we wanted to verify whether drug-resistant human neuroblastoma SK-N-BE(2) cells respond to a PEMF in a similar fashion. Materials and methods SK-N-BE(2) cells were exposed to repeated 2 mT, 75 Hz PEMF (15 min each, repeated 3 times over 5 days), and ROS production, Mn-dependent superoxide dismutase (MnSOD)-based antioxidant protection and viability were assessed after 10 min or 30 min 1 mM hydrogen peroxide. Sham controls were kept at the same time in identical cell culture incubators. Results The PEMF increased the MnSOD-based antioxidant protection and reduced the ROS production in response to a pro-oxidant challenge. Conclusions Our work might lay foundation for the development of non-invasive PEMF-based approaches aimed at elevating endogenous antioxidant properties in cellular or tissue models.
authors.affiliation: a Department of Life, Health and Environmental Sciences , University of L'Aquila , L'Aquila, Italy ;
authors.forename: Stefano
authors.initials: S
authors.lastname: Falone
authors.affiliation: b Department of Drug Sciences , Section of Pharmacology, University of Pavia, Pavia, Italy ;
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Marchesi
authors.affiliation: b Department of Drug Sciences , Section of Pharmacology, University of Pavia, Pavia, Italy ;
authors.forename: Cecilia
authors.initials: C
authors.lastname: Osera
authors.affiliation: c Department of Electrical , Computer and Biomedical Engineering, University of Pavia, Pavia, Italy ;|d Centre for Health Technologies (C.H.T.), University of Pavia, Pavia, Italy ;
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fassina
authors.affiliation: e Department of Biology and Biotechnology , University of Pavia , Pavia , Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Comincini
authors.affiliation: b Department of Drug Sciences , Section of Pharmacology, University of Pavia, Pavia, Italy ;
authors.forename: Marialaura
authors.initials: M
authors.lastname: Amadio
authors.affiliation: b Department of Drug Sciences , Section of Pharmacology, University of Pavia, Pavia, Italy ;
authors.forename: Alessia
authors.initials: A
authors.lastname: Pascale
mesh_terms: D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D009447:Neuroblastoma / Q000378:metabolism*; D016877:Oxidants / Q000008:administration & dosage; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011829:Radiation Dosage; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D016877:Oxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: ELF-EMF; SK-N-BE(2) cells; oxidative stimulus; reactive oxygen species; superoxide dismutase