title: Growth in Egg Yolk Enhances Salmonella Enteritidis Colonization and Virulence in a Mouse Model of Human Colitis.
abstract: Salmonella Enteritidis (SE) is one of the most common causes of bacterial food-borne illnesses in the world. Despite the SE's ability to colonize and infect a wide-range of host, the most common source of infection continues to be the consumption of contaminated shell eggs and egg-based products. To date, the role of the source of SE infection has not been studied as it relates to SE pathogenesis and resulting disease. Using a streptomycin-treated mouse model of human colitis, this study examined the virulence of SE grown in egg yolk and Luria Bertani (LB) broth, and mouse feces collected from mice experimentally infected with SEE1 (SEE1 passed through mice). Primary observations revealed that the mice infected with SE grown in egg yolk displayed greater illness and disease markers than those infected with SE passed through mice or grown in LB broth. Furthermore, the SE grown in egg yolk achieved higher rates of colonization in the mouse intestines and extra-intestinal organs of infected mice than the SE from LB broth or mouse feces. Our results here indicate that the source of SE infection may contribute to the overall pathogenesis of SE in a second host. These results also suggest that reservoir-pathogen dynamics may be critical for SE's ability to establish colonization and priming for virulence potential.
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Moreau
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Dona Saumya S
authors.initials: DS
authors.lastname: Wijetunge
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Megan L
authors.initials: ML
authors.lastname: Bailey
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Sudharsan R
authors.initials: SR
authors.lastname: Gongati
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Goodfield
authors.affiliation: Department of Food Science, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Eranda Mangala K Kurundu
authors.initials: EM
authors.lastname: Hewage
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.|Animal Resource Program, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Kennett
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Christine
authors.initials: C
authors.lastname: Fedorchuk
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Yury V
authors.initials: YV
authors.lastname: Ivanov
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Linder
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.|Animal Diagnostic Laboratory, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Bhushan M
authors.initials: BM
authors.lastname: Jayarao
authors.affiliation: Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, United States of America.|Animal Diagnostic Laboratory, The Pennsylvania State University, University Park, PA, United States of America.
authors.forename: Subhashinie
authors.initials: S
authors.lastname: Kariyawasam
mesh_terms: D000818:Animals; D002645:Chickens; D003092:Colitis / Q000382:microbiology*; D004195:Disease Models, Animal; D004530:Egg Yolk / Q000378:metabolism / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces; D005516:Food Microbiology; D006801:Humans; D007422:Intestines / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D012480:Salmonella Infections / Q000382:microbiology / Q000635:transmission; D012481:Salmonella Infections, Animal / Q000382:microbiology* / Q000635:transmission; D012477:Salmonella enteritidis / Q000472:pathogenicity*; D013307:Streptomycin / Q000737:chemistry; D014774:Virulence
chemical_list: D013307:Streptomycin