title: Effects of Nosema apis, N. ceranae, and coinfections on honey bee (Apis mellifera) learning and memory.
abstract: Western honey bees (Apis mellifera) face an increasing number of challenges that in recent years have led to significant economic effects on apiculture, with attendant consequences for agriculture. Nosemosis is a fungal infection of honey bees caused by either Nosema apis or N. ceranae. The putative greater virulence of N. ceranae has spurred interest in understanding how it differs from N. apis. Little is known of effects of N. apis or N. ceranae on honey bee learning and memory. Following a Pavlovian model that relies on the proboscis extension reflex, we compared acquisition learning and long-term memory recall of uninfected (control) honey bees versus those inoculated with N. apis, N. ceranae, or both. We also tested whether spore intensity was associated with variation in learning and memory. Neither learning nor memory differed among treatments. There was no evidence of a relationship between spore intensity and learning, and only limited evidence of a negative effect on memory; this occurred only in the co-inoculation treatment. Our results suggest that if Nosema spp. are contributing to unusually high colony losses in recent years, the mechanism by which they may affect honey bees is probably not related to effects on learning or memory, at least as assessed by the proboscis extension reflex.
authors.affiliation: Department of Biology, Acadia University, Wolfville, NS, Canada, B4P 2R6.
authors.forename: Lise R
authors.initials: LR
authors.lastname: Charbonneau
authors.affiliation: Department of Biology, Acadia University, Wolfville, NS, Canada, B4P 2R6.
authors.forename: Neil Kirk
authors.initials: NK
authors.lastname: Hillier
authors.affiliation: Bayer CropScience Ecotoxicology, 2 T. W. Alexander Drive, Research Triangle Park, NC, USA, 27709.
authors.forename: Richard E L
authors.initials: RE
authors.lastname: Rogers
authors.affiliation: Department of Biology, Acadia University, Wolfville, NS, Canada, B4P 2R6.|Department of Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R2.
authors.forename: Geoffrey R
authors.initials: GR
authors.lastname: Williams
authors.affiliation: Department of Biology, Acadia University, Wolfville, NS, Canada, B4P 2R6.
authors.forename: Dave
authors.initials: D
authors.lastname: Shutler
mesh_terms: D000818:Animals; D001516:Bees / Q000382:microbiology* / Q000502:physiology; D060085:Coinfection / Q000503:physiopathology; D057567:Memory, Long-Term; D016881:Microsporidiosis / Q000503:physiopathology*; D016818:Nosema / Q000472:pathogenicity; D012018:Reflex