title: Intravenous Administration of Lycopene, a Tomato Extract, Protects against Myocardial Ischemia-Reperfusion Injury.
abstract: BACKGROUND
  Oral uptake of lycopene has been shown to be beneficial for preventing myocardial ischemia-reperfusion (I/R) injury. However, the strong first-pass metabolism of lycopene influences its bioavailability and impedes its clinic application. In this study, we determined an intravenous (IV) administration dose of lycopene protects against myocardial infarction (MI) in a mouse model, and investigated the effects of acute lycopene administration on reactive oxygen species (ROS) production and related signaling pathways during myocardial I/R.
  
  
  METHODS
  In this study, we established both in vitro hypoxia/reoxygenation (H/R) cell model and in vivo regional myocardial I/R mouse model by ligating left anterior artery descending. TTC dual staining was used to assess I/R induced MI in the absence and presence of acute lycopene administration via tail vein injection.
  
  
  RESULTS
  Lycopene treatment (1 μM) before reoxygenation significantly reduced cardiomyocyte death induced by H/R. Intravenous administration of lycopene to achieve 1 μM concentration in circulating blood significantly suppressed MI, ROS production, and JNK phosphorylation in the cardiac tissue of mice during in vivo regional I/R.
  
  
  CONCLUSIONS
  Elevating circulating lycopene to 1 μM via IV injection protects against myocardial I/R injury through inhibition of ROS accumulation and consequent inflammation in mice.
authors.affiliation: Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, Chongqing 400016, China. chaotongcqmu@163.com.|Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. chaotongcqmu@163.com.|Chemical Biology Research Center, College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China. chaotongcqmu@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Tong
authors.affiliation: Laboratory of Lipid &amp; Glucose Metabolism, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. pengchuan2096@sina.com.
authors.forename: Chuan
authors.initials: C
authors.lastname: Peng
authors.affiliation: Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. llian_w@163.com.
authors.forename: Lianlian
authors.initials: L
authors.lastname: Wang
authors.affiliation: Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. Morcherl@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. xiaotaocqmu@163.com.
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. aaa.abing@foxmail.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. shinydl@163.com.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, Chongqing 400016, China. thibautdelplancke@gmail.com.|Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. thibautdelplancke@gmail.com.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Delplancke
authors.affiliation: Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, Chongqing 400016, China. zh2844@gmail.com.|Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. zh2844@gmail.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Yuzhong District, Chongqing 400016, China. qihongbo728@163.com.|Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, Chongqing 400016, China. qihongbo728@163.com.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Qi
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D000975:Antioxidants / Q000008:administration & dosage* / Q000302:isolation & purification; D002338:Carotenoids / Q000008:administration & dosage* / Q000302:isolation & purification; D016923:Cell Death / Q000187:drug effects; D019610:Cytoprotection; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D008297:Male; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000378:metabolism / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation; D008517:Phytotherapy; D010946:Plants, Medicinal; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D002338:Carotenoids; D048031:JNK Mitogen-Activated Protein Kinases; D000077276:Lycopene
keywords: ROS; inflammation; ischemia-reperfusion injury; lycopene; myocardial infarction