title: Ecological changes in oral microcosm biofilm during maturation.
abstract: The aim of this study was to evaluate the ecological changes in the biofilm at different stages of maturation using 16S rDNA gene amplicon sequencing and to identify correlations between red/green (R/G) fluorescence ratio and ecological changes. An oral microcosm biofilm was initiated from the saliva of a single donor and grown anaerobically for up to 10 days in basal medium mucin. Quantitative light-induced fluorescence analysis was shown that the R/G ratio of the biofilm increased consistently, but the slope rapidly decreased after six days. The bacterial compositions of 10 species also consistently changed over time. However, there was no significant correlation between each bacteria and red fluorescence. The monitoring of the maturation process of oral microcosm biofilm over 10 days revealed that the R/G ratio and the bacterial composition within biofilm consistently changed. Therefore, the R/G fluorescence ratio of biofilm may be related with its ecological change rather than specific bacteria
authors.affiliation: Kyungpook National University, Department of Dental Hygiene, 2559 Gyeongsang-daero, Sangju 37224, Republic of Korea.
authors.forename: Young-Seok
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Yonsei University College of Dentistry, BK 21 PLUS Project, Department of Preventive Dentistry and Public Oral Health, 50 Yonsei-ro, Seoul 03722, Republic of Korea.
authors.forename: Si-Mook
authors.initials: SM
authors.lastname: Kang
authors.affiliation: Yonsei University College of Dentistry, BK 21 PLUS Project, Department of Preventive Dentistry and Public Oral Health, 50 Yonsei-ro, Seoul 03722, Republic of Korea.
authors.forename: Eun-Song
authors.initials: ES
authors.lastname: Lee
authors.affiliation: Yonsei University College of Dentistry, Department of Oral Biology, 50 Yonsei-ro, Seoul 03722, Republic of Korea.
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Yonsei University College of Dentistry, BK 21 PLUS Project, Department of Preventive Dentistry and Public Oral Health, 50 Yonsei-ro, Seoul 03722, Republic of Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Kim
authors.affiliation: Yonsei University College of Dentistry, BK 21 PLUS Project, Department of Preventive Dentistry and Public Oral Health, 50 Yonsei-ro, Seoul 03722, Republic of Korea.
authors.forename: Baek-Il
authors.initials: BI
authors.lastname: Kim
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D018441:Biofilms / Q000254:growth & development*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008954:Models, Biological; D012463:Saliva / Q000382:microbiology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014070:Tooth / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial