title: Characterization of genetically engineered mouse models carrying Col2a1-cre-induced deletions of Lrp5 and/or Lrp6.
abstract: Mice carrying Collagen2a1-cre-mediated deletions of Lrp5 and/or Lrp6 were created and characterized. Mice lacking either gene alone were viable and fertile with normal knee morphology. Mice in which both Lrp5 and Lrp6 were conditionally ablated via Collagen2a1-cre-mediated deletion displayed severe defects in skeletal development during embryogenesis. In addition, adult mice carrying Collagen2a1-cre-mediated deletions of Lrp5 and/or Lrp6 displayed low bone mass suggesting that the Collagen2a1-cre transgene was active in cells that subsequently differentiated into osteoblasts. In both embryonic skeletal development and establishment of adult bone mass, Lrp5 and Lrp6 carry out redundant functions.
authors.affiliation: Center for Cancer and Cell Biology, Program in Skeletal Disease and Tumor Microenvironment, Van Andel Research Institute , Grand Rapids MI 49503, USA.
authors.forename: Cassie A
authors.initials: CA
authors.lastname: Schumacher
authors.affiliation: Center for Cancer and Cell Biology, Program in Skeletal Disease and Tumor Microenvironment, Van Andel Research Institute , Grand Rapids MI 49503, USA.
authors.forename: Danese M
authors.initials: DM
authors.lastname: Joiner
authors.affiliation: Center for Cancer and Cell Biology, Program in Skeletal Disease and Tumor Microenvironment, Van Andel Research Institute , Grand Rapids MI 49503, USA.
authors.forename: Kennen D
authors.initials: KD
authors.lastname: Less
authors.affiliation: Center for Cancer and Cell Biology, Program in Skeletal Disease and Tumor Microenvironment, Van Andel Research Institute , Grand Rapids MI 49503, USA.
authors.forename: Melissa Oosterhouse
authors.initials: MO
authors.lastname: Drewry
authors.affiliation: Center for Cancer and Cell Biology, Program in Skeletal Disease and Tumor Microenvironment, Van Andel Research Institute , Grand Rapids MI 49503, USA.
authors.forename: Bart O
authors.initials: BO
authors.lastname: Williams