title: Next-generation proteomics faces new challenges in environmental biotechnology.
abstract: Environmental biotechnology relies on the exploration of novel biological systems and a thorough understanding of the underlying molecular mechanisms. Next-generation proteomics based on the latest generation of mass analyzers currently allows the recording of complete proteomes from any microorganism. Interpreting these data can be straightforward if the genome of the organism is established, or relatively easy to perform through proteogenomics approaches if a draft sequence can be obtained. However, next-generation proteomics faces new, interesting challenges when the organism is distantly related to previously characterized organisms or when mixtures of organisms have to be analyzed. New mass spectrometers and innovative bioinformatics tools are reshaping the possibilities of homology-based proteomics, proteogenomics, and metaproteomics for the characterization of biological systems. Novel time- and cost-effective screening strategies are also possible with this methodology, as exemplified by whole proteome thermal profiling and subpopulation proteomics. The complexity of environmental samples allows for unique developments of approaches and concepts.
authors.affiliation: CEA, DRF, IBiTec-S, SPI, Li2D, Laboratory "Innovative Technologies for Detection and Diagnostics", Bagnols-sur-Cèze F-30200, France. Electronic address: jean.armengaud@cea.fr.
authors.forename: Jean
authors.initials: J
authors.lastname: Armengaud
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods*; D016678:Genome; D020543:Proteome / Q000235:genetics; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome