title: A highly sensitive aptasensor for OTA detection based on hybridization chain reaction and fluorescent perylene probe.
abstract: An optical aptasensor was developed for ultrasensitive detection of ochratoxin A (OTA) based on hybridization chain reaction (HCR) amplification strategy and fluorescent perylene probe (PAPDI)/DNA composites. Dendritic DNA concatamers were synthesized by HCR strategy and modified on magnetic nanoparticles through aptamer as medium. A large amount of PAPDI probe aggregated under the induction of DNA concatamers and caused fluorescence quenching. In the presence of OTA, the PAPDI/DNA composites were released from magnetic nanoparticles due to the strong affinity between aptamer and OTA. In ethanol, PAPDI monomers disaggregated and produced strong fluorescence. The present method displays excellent sensitivity and selectivity towards OTA.
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Wuhan University, Wuhan 430072, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China. Electronic address: bwang@swu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China.
authors.forename: Yuanya
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China. Electronic address: bweng1984@gmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Weng
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Xu
authors.affiliation: Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Li
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D057230:Limit of Detection; D009183:Mycotoxins / Q000032:analysis*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009793:Ochratoxins / Q000032:analysis*; D010569:Perylene / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D003313:Zea mays / Q000382:microbiology
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A; D010569:Perylene
keywords: Aptamer; Hybridization chain reaction; OTA; Optical aptasensor; Perylene probe