title: Soluble Human Intestinal Lactoferrin Receptor: Ca(2+)-Dependent Binding to Sepharose-Based Matrices.
abstract: A soluble form of human intestinal lactoferrin receptor (shLFR) is identical to human intelectin-1 (hITLN-1), a galactofuranose-binding protein that acts as a host defense against invading pathogenic microorganisms. We found that recombinant shLFR, expressed in mammalian cells (CHO DG44, COS-1, and RK13), binds tightly to Sepharose 4 Fast Flow (FF)-based matrices in a Ca(2+)-dependent manner. This binding of shLFR to Sepharose 4 FF-based matrices was inhibited by excess D-galactose, but not by D-glucose, suggesting that shLFR recognizes repeating units of α-1,6-linked D-galactose in Sepharose 4 FF. Furthermore, shLFR could bind to both Sepharose 4B- and Sepharose 6B-based matrices that were not crosslinked in a similar manner as to Sepharose 4 FF-based matrices. Therefore, shLFR (hITLN-1) binds to Sepharose-based matrices in a Ca(2+)-dependent manner. This binding property is most likely related to the ability, as host defense lectins, to recognize sepharose (agarobiose)-like structures present on the surface of invading pathogenic microorganisms.
authors.affiliation: School of Bioscience and Biotechnology, Tokyo University of Technology.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Oshima
authors.forename: Kohei
authors.initials: K
authors.lastname: Seki
authors.forename: Masataka
authors.initials: M
authors.lastname: Shibuya
authors.forename: Yuki
authors.initials: Y
authors.lastname: Naka
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sato
mesh_terms: D000818:Animals; D016466:CHO Cells; D019556:COS Cells; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003412:Cricetulus; D006801:Humans; D007422:Intestines; D011485:Protein Binding; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012685:Sepharose / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C038340:lactoferrin receptors; D012685:Sepharose; D002118:Calcium