title: Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells.
abstract: Urine-derived stem cells (USCs) are isolated from voided urine and display high proliferative activity and multiple differentiation potentials. The applicability of USCs in the treatment of bladder dysfunction and in cell-based urological tissue engineering has been demonstrated. Whether they could serve as a potential stem cell source for the treatment of diabetes mellitus (DM) and its complications has not been investigated. Here, we report the repairing and protective effects of USCs on pancreatic islets, the myocardium, the renal glomerulus and the bladder detrusor in diabetic rat models. Type 2 diabetic rat models were induced by means of a high fat diet and intraperitoneal injection with streptozotocin. USCs isolated from voided urine were administered via tail veins. The functional changes of pancreatic islets, left ventricle, glomerulus and bladder micturition were assessed by means of insulin tolerance tests, echocardiography, urine biochemical indexes and cystometry. The histologic changes were evaluated by hematoxylin and eosin staining, Masson's trichrome staining and TUNEL staining. Treatment with USCs significantly alleviated the histological destruction and functional decline. Although the USC treatment did not decrease fasting blood glucose to a significantly different level, the fibrosis and apoptosis of the myocardium, glomerulus and detrusor were significantly inhibited. This study indicates that administration of USCs may be useful for the treatment of the complications of DM.
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xingyou
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jingzhen
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Bishao
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Ding
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Wake Forest Institute of Regenerative Medicine, Wake Forest University, Winston Salem, NC, USA.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Urology, Second Affiliated Hospital, Third Military Medical University, Chongqing, China. Electronic address: lilongk@hotmail.com.
authors.forename: Longkun
authors.initials: L
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis; D048909:Diabetes Complications / Q000473:pathology / Q000628:therapy*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000628:therapy; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology / Q000628:therapy*; D005355:Fibrosis / Q000517:prevention & control; D006801:Humans; D007515:Islets of Langerhans / Q000473:pathology; D007678:Kidney Glomerulus / Q000473:pathology; D008297:Male; D039902:Multipotent Stem Cells / Q000637:transplantation*; D009206:Myocardium / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous; D001743:Urinary Bladder / Q000473:pathology; D014556:Urine / Q000166:cytology*; D055815:Young Adult
keywords: Apoptosis; Diabetes mellitus; Diabetic cardiomyopathy; Diabetic cystopathy; Diabetic nephropathy; Fibrosis; Urine-derived stem cell