title: Dual-Target Electrochemical Biosensing Based on DNA Structural Switching on Gold Nanoparticle-Decorated MoS2 Nanosheets.
abstract: A MoS2-based electrochemical aptasensor has been developed for the simultaneous detection of thrombin and adenosine triphosphate (ATP) based on gold nanoparticles-decorated MoS2 (AuNPs-MoS2) nanocomposites. Two different aptamer probes labeled with redox tags were simultaneously immobilized on an AuNPs-MoS2 film modified electrode via Au-S bonds. The aptamers presented structural switches with the addition of target molecules (thrombin and ATP), resulting in methylene blue (MB) far from or ferrocene (Fc) close to the electrode surface. Therefore, a dual signaling detection strategy was developed, which featured both "signal-on" and "signal-off" elements in the detection system because of the target-induced structure switching. This proposed aptasensor could simultaneously determine ATP and thrombin as low as 0.74 nM ATP and 0.0012 nM thrombin with high selectivity, respectively. In addition, thrombin and ATP could act as inputs to activate an AND logic gate.
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications , 9 Wenyuan Road, Nanjing, Jiangsu 210023, China.
authors.forename: Shao
authors.initials: S
authors.lastname: Su
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications , 9 Wenyuan Road, Nanjing, Jiangsu 210023, China.
authors.forename: Haofan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications , 9 Wenyuan Road, Nanjing, Jiangsu 210023, China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Cao
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications , 9 Wenyuan Road, Nanjing, Jiangsu 210023, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.affiliation: Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Peng
authors.affiliation: Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications , 9 Wenyuan Road, Nanjing, Jiangsu 210023, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Yuwen
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications , 9 Wenyuan Road, Nanjing, Jiangsu 210023, China.|Division of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications , 9 Wenyuan Road, Nanjing, Jiangsu 210023, China.
authors.forename: Lianhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D008982:Molybdenum / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis*
chemical_list: D004220:Disulfides; D008567:Membranes, Artificial; D006046:Gold; D008982:Molybdenum; D000255:Adenosine Triphosphate; D004247:DNA; D013917:Thrombin; C082964:molybdenum disulfide
keywords: aptasensor; gold nanoparticles; logic gates; molybdenum disulfide; simultaneous detection