title: [Development of transgenic maize with anti-rough dwarf virus artificial miRNA vector and their disease resistance].
abstract: Maize is one of the most important food crops. Rice black-streaked dwarf virus is a maize rough dwarf disease pathogen. The occurrence and transmission of maize rough dwarf disease brings great damage to maize production. The technology of using artificial miRNA to build antiviral plant has been proven effective in a variety of plants. However, such trials in maize have not been reported. We designed primers based on the sequence of maize zea-miR159a precursor and sequence of function protein genes and silencing RBSDV coding genes in RBSDV genome. We constructed amiRNA (artificial miRNA) gene for silencing RBSDV coding gene and gene silencing suppressor. We constructed pCAMBIA3301-121-amiRNA plant expression vector for transforming maize inbred lines Z31 by using agrobacterium mediated method. After molecular analysis of transgenic maize, homozygous lines with high miRNA expression were selected by molecular detection for a subsequent natural infection experiment. We studied the severity of maize rough dwarf disease according to a grading standard (grade 0 to 4). The experiment results showed that the disease resistance of transgenic homozygous maize with the anti-rough dwarf virus amiRNA vector was better than that of wild type. Among the transgenic maize, S6-miR159 transgenic maize had high disease resistance. It is feasible to create new maize variety by the use of artificial miRNA.
authors.forename: Ning
authors.initials: N
authors.lastname: Xuan
authors.forename: Chuanzhi
authors.initials: C
authors.lastname: Zhao
authors.forename: Zhenying
authors.initials: Z
authors.lastname: Peng
authors.forename: Gao
authors.initials: G
authors.lastname: Chen
authors.forename: Fei
authors.initials: F
authors.lastname: Bian
authors.forename: Mingzheng
authors.initials: M
authors.lastname: Lian
authors.forename: Guoxia
authors.initials: G
authors.lastname: Liu
authors.forename: Xingjun
authors.initials: X
authors.lastname: Wang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Bi
mesh_terms: D060467:Disease Resistance / Q000235:genetics*; D020868:Gene Silencing; D005822:Genetic Vectors; D035683:MicroRNAs / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics; D012087:Reoviridae / Q000472:pathogenicity*; D003313:Zea mays / Q000235:genetics*
chemical_list: D035683:MicroRNAs