title: Toll-like receptor 2 of tongue sole Cynoglossus semilaevis: Signaling pathway and involvement in bacterial infection.
abstract: Toll-like receptor (TLR) 2 is a member of the TLR family that plays a pivotal role in innate immunity. In mammals, TLR2 is known to recognize specific microbial structures and trigger MyD88-dependent signaling to induce various cytokine responses. In this study, we examined the expression and function of the tongue sole Cynoglossus semilaevis TLR2, CsTLR2. CsTLR2 is composed of 898 amino acid residues and shares 25.6%-27.3% overall sequence identities with known teleost TLR2. CsTLR2 is a transmembrane protein with a toll/interleukin-1 receptor domain and eight leucine-rich repeats. Expression of CsTLR2 occurred in multiple tissues and was upregulated during bacterial infection. Stimulation of the CsTLR2 pathway led to enhanced expression of MyD88-dependent signaling molecules. Recombinant CsTLR2 (rCsTLR2) corresponding to the extracellular region was able to bind to a wide range of bacteria. Under both in vitro and in vivo conditions, rCsTLR2 significantly reduced bacterial infection. These observations add new insights into the signaling and function of teleost TLR2.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Xue-Peng
authors.initials: XP
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: lsun@qdio.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D058491:Bacterial Load; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D020609:Edwardsiella tarda; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology / Q000517:prevention & control; D029941:Fish Proteins / Q000276:immunology*; D005412:Flatfishes / Q000235:genetics / Q000276:immunology*; D007668:Kidney / Q000378:metabolism; D055666:Lipopeptides / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D013154:Spleen / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D055666:Lipopeptides; D053594:Myeloid Differentiation Factor 88; C508075:Pam(3)CSK(4) peptide; D012333:RNA, Messenger; D011994:Recombinant Proteins; D051195:Toll-Like Receptor 2
keywords: Bacterial infection; Cynoglossus semilaevis; MyD88; Toll-like receptor