title: Fabricating photoelectrochemical aptasensor for selectively monitoring microcystin-LR residues in fish based on visible light-responsive BiOBr nanoflakes/N-doped graphene photoelectrode.
abstract: The presence of microcystins in fish has been augmenting the risk of toxicity to animal and human health. Herein, a selective and sensitive method for detecting microcystin-LR (MC-LR) in fish samples by integrating the photoelectrochemical (PEC) technique and the specific recognition ability of aptamer was developed. Specifically, as an efficient PEC transducer, the BiOBr nanoflakes/N-doped graphene p-n heterojunction electrode was utilized as the aptamer immobilization platform via the π-π stacking interaction, which would be a biosensor enabling the convenient and exquisite PEC analysis. Subsequently, the PEC response of constructed aptasensor was specific binding to MC-LR. Other isoforms did not interfere with the detection process, and thus, it could be applied for the highly selective determination of MC-LR level. Under the optimized condition, the PEC signal versus the logarithm of the MC-LR concentration was in good linear relationship ranging from 0.1pM to 100nM with detection limit about 0.03pM. The constructed method was employed to analyze fish samples collected from the local supermarket. The overall analytical recovery of MC-LR in the fish matrices ranged from 97.8 to 101.6%, with relative standard deviation (RSD) of 2.52-5.14%, implying it would have great potential in farm product analysis.
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Du
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Ding
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Liming
authors.initials: L
authors.lastname: Dai
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Hao
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Baijing
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: wangkun@ujs.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001729:Bismuth / Q000737:chemistry*; D000458:Cyanobacteria / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D005399:Fishes / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008027:Light; D057230:Limit of Detection; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins / Q000032:analysis*; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D017747:Seafood / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D008387:Marine Toxins; D052998:Microcystins; D006108:Graphite; C057862:cyanoginosin LR; C542279:bismuth oxybromide; D001729:Bismuth
keywords: Aptasensor; BiOBr nanoflakes; Microcystin-LR; N-doped graphene; Photoelectrochemical; P–N heterojunction