title: An alternative mini buffy coat preparation method for adult patients with extracorporeal photopheresis contraindications.
abstract: BACKGROUND
  Extracorporeal photopheresis (ECP) is an important cell-based therapy for various diseases but is limited to patients eligible for apheresis. We developed an alternative mini buffy coat (BC) preparation method using the Spectra Optia® apheresis system and compared its efficacy of white blood cell (WBC) recovery with the standard mini BC preparation method already established for pediatric patients.
  
  
  METHODS
  Whole blood (450 ± 45 mL) samples were collected from 30 randomly selected healthy volunteer blood donors and divided into two groups. In the first group, WBCs were separated with a fully automated separator device (Compomat G4®). In the second group, BCs were separated with the bone marrow processing program of the Spectra Optia apheresis system.
  
  
  RESULTS
  There were no significant differences in total leukocyte counts per product between the two groups. In contrast, lymphocyte counts per product were significantly higher (P < 0.001) in BCs separated from apheresis.
  
  
  CONCLUSIONS
  Our novel technique resulted in similar WBC yields but higher lymphocyte yields than the standard mini BC preparation method. This method can serve as an alternative to WBC collection in conventional ECP for adult patients with apheresis contraindications. J. Clin. Apheresis 32:12-15, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Grabmer
authors.affiliation: Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.
authors.forename: Sandra
authors.initials: S
authors.lastname: Schlager
authors.affiliation: Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.
authors.forename: Georg
authors.initials: G
authors.lastname: Mayer
authors.affiliation: Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Streif
authors.affiliation: Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lener
authors.affiliation: Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.|Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Schallmoser
authors.affiliation: Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.|Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University (PMU), Salzburg, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Rohde
mesh_terms: D000328:Adult; D058621:Blood Buffy Coat / Q000166:cytology*; D001781:Blood Component Removal / Q000379:methods / Q000592:standards; D002469:Cell Separation / Q000379:methods; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology; D018655:Lymphocyte Count; D017893:Photopheresis / Q000379:methods* / Q000592:standards
keywords: ECP; apheresis; mini buffy coat; photopheresis