title: Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae).
abstract: OBJECTIVE
  Veronicastrum japonicum (Plantaginaceae) grows in grasslands on Honshu Island, Japan, and is threatened by habitat loss because of rapid land development over recent decades. For the genetic characterization of the remaining populations, microsatellite markers were developed.
  
  
  RESULTS
  Twelve polymorphic microsatellite loci were developed using next-generation sequencing. The number of alleles per locus ranged from two to 24 (mean 7.7), and the expected heterozygosity per locus ranged from 0.35 to 0.94 (mean 0.68).
  
  
  CONCLUSIONS
  These markers can be used for genetic studies in conservation, such as the evaluation of genetic diversity and genetic structure.
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Nakahama
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Izuno
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Kurumi
authors.initials: K
authors.lastname: Arima
authors.affiliation: Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Isagi
keywords: Plantaginaceae; Veronicastrum japonicum; conservation; grassland herb; next-generation sequencing