title: Evidence for the absence of visceral pain in C57BL6/N mice subjected to therapeutically relevant O3/O2-pneumoperitoneum.
abstract: Each different gas that is used to induce a pneumoperitoneum (PP) exhibits individual effects within the peritoneal cavity. This might include adverse effects such as pain and/or inflammatory reactions. The acute effects of ozonized oxygen (O3/O2), a highly oxidative gas mixture, after being insufflated into the peritoneal cavity are analysed in this study. Using the abdominal constriction response ('writhing') assay of chemical nociception in C57BL6/N mice, O3/O2-PP was found not to be associated with visible pain responses and did not alter the c-fos expression in the spinal cord. In addition, mRNA expression levels of the pro-inflammatory cytokines, interleukin (IL)-1β and IL-6, were found unaltered in the spleen 2 h after insufflation. In conclusion, O3/O2-PP is free of adverse pain and does not trigger inflammatory immune responses.
authors.affiliation: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Bette
authors.affiliation: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Springmann
authors.affiliation: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Rossmann
authors.affiliation: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Weihe
authors.affiliation: Department of Molecular Neuroscience, Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Schütz
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000494:pharmacology; D010126:Ozone / Q000494:pharmacology*; D011027:Pneumoperitoneum / Q000139:chemically induced / Q000503:physiopathology; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D059265:Visceral Pain / Q000209:etiology / Q000503:physiopathology*
chemical_list: D016207:Cytokines; D016760:Proto-Oncogene Proteins c-fos; D010126:Ozone; D010100:Oxygen
keywords: cytokine; ozone; pain; pneumoperitoneum; refinement