title: Supporting data for analysis of the Helicobacter pylori exoproteome.
abstract: The goal of this research was to analyze the composition of the Helicobacter pylori exoproteome at multiple phases of bacterial growth (Snider et al., 2015) [1]. H. pylori was grown in a serum-free medium and at serial time points, aliquots were centrifuged and fractionated to yield culture supernatant, a soluble cellular fraction, and a membrane fraction. Samples were analyzed by single dimensional LC-MS/MS analyses and multidimensional protein identification technology (MudPIT). Here we present data showing the numbers of assigned spectra and proportional abundance of individual proteins in each of the samples analyzed, along with a calculation of the level of enrichment of individual proteins in the supernatant compared to the soluble cellular fraction.
authors.affiliation: Department of Medicine, Nashville, TN, USA.
authors.forename: Christina A
authors.initials: CA
authors.lastname: Snider
authors.affiliation: Department of Pathology, Microbiology and Immunology, Nashville, TN, USA.
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Voss
authors.affiliation: Proteomics Laboratory, Mass Spectrometry Research Center, Nashville, TN, USA; Department of Biochemistry, Nashville, TN, USA.
authors.forename: W Hayes
authors.initials: WH
authors.lastname: McDonald
authors.affiliation: Department of Medicine, Nashville, TN, USA; Department of Pathology, Microbiology and Immunology, Nashville, TN, USA; Vanderbilt University School of Medicine; Veterans Affairs Tennessee Valley Healthcare System, Nashville, TN, USA.
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Cover
keywords: Bacterial protein secretion; Exoproteome; Gastric cancer; Mass spectrometry; Peptic ulcer disease; Proteomics; Secretome