title: Reactive oxygen species production and antioxidant enzyme activity during epididymal sperm maturation in Corynorhinus mexicanus bats.
abstract: Prolonged sperm storage in the epididymis of Corynorhinus mexicanus bats after testicular regression has been associated with epididymal sperm maturation in the caudal region, although the precise factors linked with this phenomenon are unknown. The aim of this work is to determine the role of reactive oxygen species (ROS) and changes in antioxidant enzymatic activity occurring in the spermatozoa and epididymal fluid over time, in sperm maturation and storage in the caput, corpus and cauda of the bat epididymis. Our data showed that an increment in ROS production coincided with an increase in superoxide dismutase (SOD) activity in epididymal fluid and with a decrease in glutathione peroxidase (GPX) activity in the spermatozoa in at different time points and epididymal regions. The increase in ROS production was not associated with oxidative damage measured by lipid peroxidation. The results of the current study suggest the existence of a shift in the redox balance, which might be associated with sperm maturation and storage.
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San Rafael Atlixco No. 186, Colonia Vicentina, CP 09340 México, D.F., Mexico. Electronic address: editharenas2000@yahoo.com.mx.
authors.forename: Edith
authors.initials: E
authors.lastname: Arenas-Ríos
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San Rafael Atlixco No. 186, Colonia Vicentina, CP 09340 México, D.F., Mexico.
authors.forename: Adolfo
authors.initials: A
authors.lastname: Rosado García
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San Rafael Atlixco No. 186, Colonia Vicentina, CP 09340 México, D.F., Mexico.
authors.forename: Edith
authors.initials: E
authors.lastname: Cortés-Barberena
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San Rafael Atlixco No. 186, Colonia Vicentina, CP 09340 México, D.F., Mexico.
authors.forename: Mina
authors.initials: M
authors.lastname: Königsberg
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San Rafael Atlixco No. 186, Colonia Vicentina, CP 09340 México, D.F., Mexico.
authors.forename: Marcela
authors.initials: M
authors.lastname: Arteaga-Silva
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San Rafael Atlixco No. 186, Colonia Vicentina, CP 09340 México, D.F., Mexico.
authors.forename: Ahiezer
authors.initials: A
authors.lastname: Rodríguez-Tobón
authors.affiliation: Laboratorio de Investigación en Reproducción Animal (FMG), Universidad Autónoma Benito Juárez de Oaxaca, Avenida Universidad S/N, Colonia 5 Señores, CP 68120 Oaxaca, Mexico.
authors.forename: Gisela
authors.initials: G
authors.lastname: Fuentes-Mascorro
authors.affiliation: Departamento de Biología, Universidad Autónoma Metropolitana-Iztapalapa, Avenida San, Rafael Atlixco No. 186, Colonia Vicentina, CP 09340 México, D.F., Mexico.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: León-Galván
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002685:Chiroptera; D004822:Epididymis / Q000033:anatomy & histology / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013079:Sperm Maturation / Q000502:physiology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Antioxidant enzymes; Bats; Epididymis; ROS; Sperm maturation