title: Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC-ESI-QTOF-Mass Spectrometry Method.
abstract: The present study was conducted to carry out the phytochemical investigation of Tanacetum gracile Hook. f. & Thomson and to develop a method for the simultaneous quantification of the isolated compounds in the extracts ofT. gracile growing in different locations. Cluster analysis rectangular similarity matrix was performed to understand the chemical fingerprinting variations in the extracts. High-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS) was used to quantify four bioactive compounds, and separation of the compounds was achieved on a reverse-phase C8 column using a mobile phase of acetonitrile: 0.1% formic acid in water with a gradient elution by maintaining the flow rate of 300 μL/min. The QTOF-MS was operated using the electro-spray ionization technique with the positive ion polarity mode. The calibration curves of four marker compounds were linear over the concentration range of 3.12-100 ng/µL (R(2)> 0.996). A specific, accurate and precise HPLC-ESI-QTOF-MS method was optimized for the determination of kaempferol, ketoplenolide, tetramethoxyflavone and artemetin both individually and simultaneously. Quantification of these chemical markers in different extracts was carried out using this validated method. Kaempferol was isolated for the first time from T. gracile.
authors.affiliation: Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Sharma
authors.affiliation: Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Chetan
authors.initials: C
authors.lastname: Kumar
authors.affiliation: Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Prabhu
authors.initials: P
authors.lastname: Dutt
authors.affiliation: Plant Biotechnology Department, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Suphla
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Naresh K
authors.initials: NK
authors.lastname: Satti
authors.affiliation: Genetic Resource & Agrotech. Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Chandra
authors.affiliation: Plant Biotechnology Department, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Surinder
authors.initials: S
authors.lastname: Kitchlu
authors.affiliation: Department of Chemistry, University of Jammu, Jammu 180006, India.
authors.forename: Satya
authors.initials: S
authors.lastname: Paul
authors.forename: Ram A
authors.initials: RA
authors.lastname: Vishwakarma
authors.affiliation: Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India mkvermadr@yahoo.com mkverma@iiim.ac.in.
authors.forename: Mahendra K
authors.initials: MK
authors.lastname: Verma
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D031203:Tanacetum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*