title: Microsatellite Analysis of the Genetic Diversity and Population Structure in Dairy Goats in Thailand.
abstract: The genetic relationships between different populations and breeds of exotic dairy goats in Thailand were studied using 12 microsatellite markers. Blood samples were obtained from 211 goats from Department of Livestock Development breeding and research farms: 29 Anglonubian (AN), 21 Alpine (AP), 23 Jamunapari (JAM), 50 Saanen (SN), and 88 Toggenburg (TG). Five of the 12 microsatellite markers were found to be polymorphic. A mean of 7.40 alleles per locus was found, with a range from 5 (SPS115 and ETH225) to 11 (TGLA122). We found 24, 27, 19, 32, and 24 alleles in the AN, AP, JAM, SN, and TG breeds, respectively; 37 alleles were present in all breeds. The mean number of alleles in each population ranged from 3.2 (ETH225 locus) to 7.6 (TGLA122 locus). Genetic variability within the breeds was moderate as evidenced by the mean expected heterozygosity of 0.539. The average observed heterozygosity across the 5 markers in all breeds was 0.529 with the maximum observed at the BM1818 locus (0.772) and the minimum at the ETH225 locus (0.248). The observed and expected heterozygosity for all breeds for the 5 microsatellite markers ranged from 0.419 to 0.772 and 0.227 to 0.792, respectively. On the basis of their means, the TGLA122 and BM1818 loci were the most suitable markers for distinguishing genetic diversity among the goats. The estimated average F is value for the breeds ranged from -0.044 (ETH225) to 0.180 (SPS115), while the estimated average F st value ranged from 0.021 (SPS115) to 0.104 (ETH10). These results indicated that TGLA122 and BM1818 markers are suitable to be used for aiding conservation and breeding improvement strategies of dairy.
authors.affiliation: Faculty of Agriculture and Life Science, Chadrakasem Rajabhat University, Bangkok 10902, Thailand ; Genetic Informatics Center, Hankyong National University, Anseong 456-749, Korea .
authors.forename: Somkiat
authors.initials: S
authors.lastname: Seilsuth
authors.affiliation: Genetic Informatics Center, Hankyong National University, Anseong 456-749, Korea .
authors.forename: Joo Hee
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Genetic Informatics Center, Hankyong National University, Anseong 456-749, Korea .
authors.forename: Hong Sik
authors.initials: HS
authors.lastname: Kong
authors.affiliation: Genetic Informatics Center, Hankyong National University, Anseong 456-749, Korea .
authors.forename: Gwang Joo
authors.initials: GJ
authors.lastname: Jeon
keywords: Dairy Goat; Genetic Diversity; Microsatellite