title: Protein adsorption using novel carboxymethyl-curdlan microspheres.
abstract: Carboxymethyl-curdlan as a water soluble curdlan derivative, was synthesized in an aqueous alkaline medium using monochloroacetic acid. Novel carboxymethyl-curdlan (CC) microspheres were prepared by the method of W/O/W emulsion. The chemical and morphological structures of CC microspheres were investigated by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR) and particle size analysis. The CC microspheres were spherical, free flowing, non-aggregated and uniform mono-disperse with diameter of 260μm. The prepared CC microspheres were applied to adsorbing Bovine serum albumin (BSA) as model protein. Factors influencing the adsorption of BSA such as solution pH, temperature, initial BSA concentration and ionic strength were examined by batch experiments. The maximum adsorption capacity was calculated as 168mg/g under optimal conditions including BSA initial concentration (4mg/mL), pH (4.7), adsorption time (9h) and temperature (35°C). The adsorption isotherm followed the Langmuir model and the adsorption kinetics fitted the pseudo-second-order model. In addition, the CC microspheres can be also regenerated and re-used.
authors.affiliation: Department of Chemical Engineering, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. Electronic address: Rafigh@engineer.com.
authors.forename: Sayyid Mahdi
authors.initials: SM
authors.lastname: Rafigh
authors.affiliation: Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Vaziri Yazdi
authors.affiliation: Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Safekordi
authors.affiliation: Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Heydari Nasab
authors.affiliation: Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ardjmand
authors.affiliation: Department of Chemistry, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Naderi
authors.affiliation: Department of Agronomy, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Mozafari
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008863:Microspheres; D009994:Osmolar Concentration; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013696:Temperature; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D047071:beta-Glucans; C063424:carboxymethylcurdlan; D012710:Serum Albumin, Bovine
keywords: Adsorption kinetics; Bovine serum albumin (BSA); Carboxymethyl-curdlan; Isotherm; Microsphere