title: Next Generation Prokaryotic Engineering: The CRISPR-Cas Toolkit.
abstract: The increasing demand for environmentally friendly production processes of green chemicals and fuels has stimulated research in microbial metabolic engineering. CRISPR-Cas-based tools for genome editing and expression control have enabled fast, easy, and accurate strain development for established production platform organisms, such as Escherichia coli and Saccharomyces cerevisiae. However, the growing interest in alternative production hosts, for which genome editing options are generally limited, requires further developing such engineering tools. In this review, we discuss established and emerging CRISPR-Cas-based tools for genome editing and transcription control of model and non-model prokaryotes, and we analyse the possibilities for further improvement and expansion of these tools for next generation prokaryotic engineering.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Mougiakos
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Elleke F
authors.initials: EF
authors.lastname: Bosma
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands; Corbion, Arkelsedijk 46, 4206 AC Gorinchem, The Netherlands.
authors.forename: Richard
authors.initials: R
authors.lastname: van Kranenburg
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands. Electronic address: john.vanderoost@wur.nl.
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D064113:CRISPR-Cas Systems; D000072669:Gene Editing / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D011995:Recombination, Genetic
keywords: CRISPR-Cas; Cas9; archaea; bacteria; genome editing; recombineering