title: Optimization of submerged fermentation medium for citrinin-free monascin production by Monascus.
abstract: Microbial fermentation of citrinin-free Monascus pigments is in favor in the development of food industry. This study investigated the influences of carbon source, nitrogen source, and mineral salts on the cell growth, monascin (MS), and citrinin (CT) production in Monascus M9. A culture medium composition was established for maximizing the production of citrinin-free MS in submerged culture, as follows: 50 g/L Japonica rice powder, 20 g/L NH4NO3, 3 g/L NaNO3, 1.5 g/L KH2PO4, 1 g/L MgSO4· 7H2O, 0.2 g/L MnSO4. Under these conditions, no CT was detectable by high performance liquid chromatography. The yield of MS reached 14.11 mg/g, improving approximately 30% compared with before optimization.
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xue
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Mianhua
authors.initials: M
authors.lastname: Chen
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Zhenjing
authors.initials: Z
authors.lastname: Li
authors.affiliation: a Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology , Tianjin University of Science and Technology , Tianjin , China.
authors.forename: Changlu
authors.initials: C
authors.lastname: Wang
mesh_terms: D002953:Citrinin / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism*; D005285:Fermentation; D006575:Heterocyclic Compounds, 3-Ring / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D032741:Monascus / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D012275:Oryza / Q000382:microbiology
chemical_list: D003470:Culture Media; D006575:Heterocyclic Compounds, 3-Ring; D009566:Nitrates; D002953:Citrinin; C006568:ammonium nitrate; C517880:monascin
keywords: Ammonium nitrate; carbon source; citrinin; mineral salts; monascin; nitrogen source