title: Disulfides with Anti-inflammatory Activity from the Brown Alga Dictyopteris membranacea.
abstract: Six new (1, 2, and 4-7) and two previously reported (3 and 8) disulfides, along with 4-butyl-2,6-cycloheptadienone, γ-tocopherol, and δ-tocopherol, were isolated from an organic extract of the brown alga Dictyopteris membranacea, collected at Gerolimenas Bay, Greece. The structure elucidation of the isolated natural products was based on analysis of their spectroscopic data. Compounds 1, 3-6, and 8 were evaluated for their antibacterial and anti-inflammatory activities. None of the compounds displayed antibacterial activity against two resistant strains of Staphylococcus aureus and one strain of Escherichia coli. In contrast, metabolite 5 was able to cause strong inhibition of NO production with an IC50 value of 3.8 μM using an LPS stimulation assay.
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, School of Health Sciences, University of Athens , Panepistimiopolis Zografou, Athens 15771, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Dimou
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, School of Health Sciences, University of Athens , Panepistimiopolis Zografou, Athens 15771, Greece.
authors.forename: Efstathia
authors.initials: E
authors.lastname: Ioannou
authors.affiliation: Department of Biochemistry, School of Medicine, University of Crete , P.O. Box 2208, Heraklion 71003, Greece.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Daskalaki
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, School of Health Sciences, University of Athens , Panepistimiopolis Zografou, Athens 15771, Greece.
authors.forename: Leto A
authors.initials: LA
authors.lastname: Tziveleka
authors.affiliation: Department of Biochemistry, School of Medicine, University of Crete , P.O. Box 2208, Heraklion 71003, Greece.
authors.forename: Sotirios C
authors.initials: SC
authors.lastname: Kampranis
authors.affiliation: Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, School of Health Sciences, University of Athens , Panepistimiopolis Zografou, Athens 15771, Greece.
authors.forename: Vassilios
authors.initials: V
authors.lastname: Roussis
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004220:Disulfides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D006115:Greece; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D000459:Phaeophyceae / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D024505:Tocopherols
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D004220:Disulfides; D008070:Lipopolysaccharides; D009569:Nitric Oxide; C479072:delta-tocopherol; D024505:Tocopherols