title: The use of fluorescently-tagged apoptolidins in cellular uptake and response studies.
abstract: The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.
authors.affiliation: Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Chong
authors.affiliation: Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: Nalin
authors.initials: N
authors.lastname: Leelatian
authors.affiliation: Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: Sean M
authors.initials: SM
authors.lastname: Deguire
authors.affiliation: Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: Asa A
authors.initials: AA
authors.lastname: Brockman
authors.affiliation: Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: David
authors.initials: D
authors.lastname: Earl
authors.affiliation: Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Ihrie
authors.affiliation: Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: Jonathan M
authors.identifier: 0000-0001-9428-8866
authors.initials: JM
authors.lastname: Irish
authors.affiliation: Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
authors.forename: Brian O
authors.initials: BO
authors.lastname: Bachmann
authors.affiliation: Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.|Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Sulikowski
mesh_terms: D000072283:A549 Cells; D001692:Biological Transport; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D018942:Macrolides; C114125:apoptolidin