title: Correlations between the CagA Antigen and Serum Levels of Anti-Helicobacter pylori IgG and IgA in Children.
abstract: We tested correlations between anti-Helicobacter pylori IgG and IgA levels and the urease test, anti-CagA protein antibody, degree of gastritis, and age. In total, 509 children (0-15 years) were enrolled. Subjects were stratified as 0-4 years (n = 132), 5-9 years (n = 274), and 10-15 years (n = 103) and subjected to the urease test, histopathology, ELISA, and western blot using whole-cell lysates of H. pylori strain 51. The positivity rate in the urease test (P = 0.003), the degree of chronic gastritis (P = 0.021), and H. pylori infiltration (P < 0.001) increased with age. The median titer for anti-H. pylori IgG was 732.5 IU/mL at 0-4 years, 689.0 IU/mL at 5-9 years, and 966.0 IU/mL at 10-15 years (P < 0.001); the median titer for anti-H. pylori IgA was 61.0 IU/mL at 0-4 years, 63.5 IU/mL at 5-9 years, and 75.0 IU/mL at 10-15 years (P < 0.001). The CagA-positivity rate was 26.5% at 0-4 years, 36.5% at 5-9 years, and 46.6% at 10-15 years for IgG (P = 0.036), and 11.3% at 0-4 years, 18.6% at 5-9 years, and 23.3% at 10-15 years for IgA (P < 0.001). Anti-H. pylori IgG and IgA titers increased with the urease test grade, chronic gastritis degree, active gastritis, and H. pylori infiltration. Presence of CagA-positivity is well correlated with a high urease test grade and high anti-H. pylori IgG/IgA levels.
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Ji-Hyun
authors.identifier: 0000-0002-0691-3957
authors.initials: JH
authors.lastname: Seo
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Chun Woo
authors.identifier: 0000-0003-4146-4349
authors.initials: CW
authors.lastname: Lim
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Ji Sook
authors.identifier: 0000-0002-4704-2246
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Jung Sook
authors.identifier: 0000-0003-0688-7493
authors.initials: JS
authors.lastname: Yeom
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Jae-Young
authors.identifier: 0000-0001-5205-202X
authors.initials: JY
authors.lastname: Lim
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Jin-Su
authors.identifier: 0000-0002-6382-6286
authors.initials: JS
authors.lastname: Jun
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Hyang-Ok
authors.identifier: 0000-0001-8849-9341
authors.initials: HO
authors.lastname: Woo
authors.affiliation: Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Hee-Shang
authors.identifier: 0000-0002-5498-838X
authors.initials: HS
authors.lastname: Youn
authors.affiliation: Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Seung-Chul
authors.identifier: 0000-0001-6033-4078
authors.initials: SC
authors.lastname: Baik
authors.affiliation: Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Woo-Kon
authors.identifier: 0000-0003-3913-2265
authors.initials: WK
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Myung-Je
authors.identifier: 0000-0002-4958-9827
authors.initials: MJ
authors.lastname: Cho
authors.affiliation: Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, Korea .
authors.forename: Kwang-Ho
authors.identifier: 0000-0002-4422-4992
authors.initials: KH
authors.lastname: Rhee
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005756:Gastritis / Q000473:pathology; D016481:Helicobacter Infections / Q000097:blood / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012720:Severity of Illness Index; D014510:Urease / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C080866:cagA protein, Helicobacter pylori; D014510:Urease
keywords: Antibodies IgG/IgA; CagA Protein; Children; Helicobacter pylori; Urease