title: Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias.
abstract: The control of spatiotemporal dynamics in biological systems is a fundamental problem in nonlinear sciences and has important applications in engineering and medicine. Optogenetic tools combined with advanced optical technologies provide unique opportunities to develop and validate novel approaches to control spatiotemporal complexity in neuronal and cardiac systems. Understanding of the mechanisms and instabilities underlying the onset, perpetuation, and control of cardiac arrhythmias will enable the development and translation of novel therapeutic approaches. Here we describe in detail the preparation and optical mapping of transgenic channelrhodopsin-2 (ChR2) mouse hearts, cardiac cell cultures, and the optical setup for photostimulation using digital light processing.
authors.affiliation: Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077, Göttingen, Germany. claudia.richter@ds.mpg.de.|DZHK German Center for Cardiovascular Research, Partnersite Göttingen, Göttingen, Germany. claudia.richter@ds.mpg.de.
authors.forename: Claudia
authors.initials: C
authors.lastname: Richter
authors.affiliation: Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077, Göttingen, Germany.|DZHK German Center for Cardiovascular Research, Partnersite Göttingen, Göttingen, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Christoph
authors.affiliation: DZHK German Center for Cardiovascular Research, Partnersite Göttingen, Göttingen, Germany.|Heart Research Center Göttingen, Clinic of Cardiology and Pulmonology, University Medical Center Göttingen, Partnersite Göttingen, Göttingen, Germany.
authors.forename: Stephan E
authors.initials: SE
authors.lastname: Lehnart
authors.affiliation: Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, Am Fassberg 17, 37077, Göttingen, Germany.|DZHK German Center for Cardiovascular Research, Partnersite Göttingen, Göttingen, Germany.|Institute for Nonlinear Dynamics, Georg-August-Universität Göttingen, Partnersite Göttingen, Göttingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Luther
mesh_terms: D000818:Animals; D001145:Arrhythmias, Cardiac / Q000175:diagnosis / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D002478:Cells, Cultured; D000075402:Channelrhodopsins; D015316:Genetic Therapy / Q000379:methods; D006321:Heart / Q000503:physiopathology / Q000528:radiation effects; D006801:Humans; D008027:Light; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D062308:Optogenetics / Q000379:methods*; D010775:Photic Stimulation / Q000379:methods; D019076:Transgenes
chemical_list: D000075402:Channelrhodopsins
keywords: Arrhythmias; Cardiac dynamics; Optical mapping; Optogenetics; Photostimulation