title: Expression of ODC Antizyme Inhibitor 2 (AZIN2) in Human Secretory Cells and Tissues.
abstract: Ornithine decarboxylase (ODC) antizyme inhibitor 2 (AZIN2), originally called ODCp, is a regulator of polyamine synthesis that we originally identified and cloned. High expression of ODCp mRNA was found in brain and testis. We reported that AZIN2 is involved in regulation of cellular vesicle transport and / or secretion, but the ultimate physiological role(s) of AZIN2 is still poorly understood. In this study we used a peptide antibody (K3) to human AZIN2 and by immunohistochemistry mapped its expression in various normal tissues. We found high expression in the nervous system, in type 2 pneumocytes in the lung, in megakaryocytes, in gastric parietal cells co-localized with H,K-ATPase beta subunit, in selected enteroendocrine cells, in acinar cells of sweat glands, in podocytes, in macula densa cells and epithelium of collecting ducts in the kidney. The high expression of AZIN2 in various cells with secretory or vesicle transport activity indicates that the polyamine metabolism regulated by AZIN2 is more significantly involved in these events than previously appreciated.
authors.affiliation: Department of Pathology, University of Helsinki, Helsinki, Finland.
authors.forename: Tiina
authors.initials: T
authors.lastname: Rasila
authors.affiliation: Department of Pathology, University of Helsinki, Helsinki, Finland.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lehtonen
authors.affiliation: Institute of Biomedicine, Anatomy, University of Helsinki, Finland.
authors.forename: Kristiina
authors.initials: K
authors.lastname: Kanerva
authors.affiliation: Department of Pathology, University of Helsinki, Helsinki, Finland.
authors.forename: Laura T
authors.initials: LT
authors.lastname: Mäkitie
authors.affiliation: Research Programs Unit, Translational Cancer Biology, University of Helsinki, Helsinki, Finland.
authors.forename: Caj
authors.initials: C
authors.lastname: Haglund
authors.affiliation: Department of Pathology, University of Helsinki, Helsinki, Finland.
authors.forename: Leif C
authors.initials: LC
authors.lastname: Andersson
mesh_terms: D000906:Antibodies / Q000276:immunology; D001921:Brain / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000378:metabolism; D005088:Exocrine Glands / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D005786:Gene Expression Regulation; D017506:H(+)-K(+)-Exchanging ATPase / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism; D008297:Male; D011073:Polyamines / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013737:Testis / Q000378:metabolism
chemical_list: D000906:Antibodies; D011073:Polyamines; D012333:RNA, Messenger; D017506:H(+)-K(+)-Exchanging ATPase; D002262:Carboxy-Lyases; C546395:AZIN2 protein, human