title: Genome-wide identification of WRKY family genes in peach and analysis of WRKY expression during bud dormancy.
abstract: Bud dormancy in deciduous fruit trees is an important adaptive mechanism for their survival in cold climates. The WRKY genes participate in several developmental and physiological processes, including dormancy. However, the dormancy mechanisms of WRKY genes have not been studied in detail. We conducted a genome-wide analysis and identified 58 WRKY genes in peach. These putative genes were located on all eight chromosomes. In bioinformatics analyses, we compared the sequences of WRKY genes from peach, rice, and Arabidopsis. In a cluster analysis, the gene sequences formed three groups, of which group II was further divided into five subgroups. Gene structure was highly conserved within each group, especially in groups IId and III. Gene expression analyses by qRT-PCR showed that WRKY genes showed different expression patterns in peach buds during dormancy. The mean expression levels of six WRKY genes (Prupe.6G286000, Prupe.1G393000, Prupe.1G114800, Prupe.1G071400, Prupe.2G185100, and Prupe.2G307400) increased during endodormancy and decreased during ecodormancy, indicating that these six WRKY genes may play a role in dormancy in a perennial fruit tree. This information will be useful for selecting fruit trees with desirable dormancy characteristics or for manipulating dormancy in genetic engineering programs.
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Tan
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China.
authors.forename: Xiling
authors.initials: X
authors.lastname: Fu
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China.
authors.forename: Xiude
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China. lilingsdau@163.com.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China. lilingsdau@163.com.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China. lilingsdau@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China. dsgao@sdau.edu.cn.|State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China. dsgao@sdau.edu.cn.|Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, 61 Daizong Road, Tai'an, 271018, China. dsgao@sdau.edu.cn.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Gao
mesh_terms: D002874:Chromosome Mapping; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005787:Gene Frequency; D018745:Genome, Plant; D010802:Phylogeny; D057445:Plant Dormancy; D010940:Plant Proteins / Q000235:genetics; D000068239:Prunus persica / Q000235:genetics* / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Bud dormancy; Peach; WRKY transcription factors