title: Levansucrase from Leuconostoc mesenteroides NTM048 produces a levan exopolysaccharide with immunomodulating activity.
abstract: OBJECTIVE
  A levansucrase from Leuconostoc mesenteroides NTM048 was cloned and expressed and its enzymatic product was characterized.
  
  
  RESULTS
  The fructansucrase gene from Leuconostoc mesenteroides was cloned and expressed in Escherichia coli. The recombinant enzyme was purified as a single protein and its properties investigated. The polymer produced by the recombinant enzyme was identified as levan by various means including TLC and NMRs, and the enzyme was identified as a GH68 levansucrase. The enzyme was optimal at pH 5.5-6 and 30 °C, and its activity was stimulated by Ca(2+). The levan produced by this strain induced IgA production in mice.
  
  
  CONCLUSIONS
  Leuconostoc mesenteroides, a probiotic strain, possessed levansucrase which catalyzed the produced levan that had immunomodulating activity.
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Ishida
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan.
authors.forename: Kouta
authors.initials: K
authors.lastname: Sakaguchi
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Matsuzaki
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Katoh
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Ishida
authors.affiliation: Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan. yamamotk@ishikawa-pu.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Nitto Pharmaceutical Industries, Ltd., Kamiueno, Muko, Kyoto, 617-0006, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Hisa
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D055162:Biocatalysis; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005630:Fructans / Q000096:biosynthesis* / Q000494:pharmacology; D006602:Hexosyltransferases / Q000235:genetics* / Q000378:metabolism*; D007070:Immunoglobulin A / Q000378:metabolism; D007155:Immunologic Factors / Q000096:biosynthesis* / Q000494:pharmacology; D000070007:Leuconostoc mesenteroides / Q000201:enzymology* / Q000235:genetics; D051379:Mice
chemical_list: D001426:Bacterial Proteins; D005630:Fructans; D007070:Immunoglobulin A; D007155:Immunologic Factors; C072599:levan; D006602:Hexosyltransferases; C021513:levansucrase; D002118:Calcium
keywords: Fructansucrase; IgA; Immunomodulating activity; Leuconostoc mesenteroides; Levan; Levansucrase