title: A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission.
abstract: Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Nam-Kyung
authors.initials: NK
authors.lastname: Yu
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.|Department of Biomedical Engineering, Sungkyunkwan University, Suwon, South Korea.
authors.forename: Hyoung F
authors.initials: HF
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Shim
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Somi
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, South Korea.
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Chuljung
authors.initials: C
authors.lastname: Kwak
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Su-Eon
authors.initials: SE
authors.lastname: Sim
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Jun-Hyeok
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Seohee
authors.initials: S
authors.lastname: Ahn
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Juyoun
authors.initials: J
authors.lastname: Yoo
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Sun-Lim
authors.initials: SL
authors.lastname: Choi
authors.affiliation: Applied Biology, College of Ecological Environment, Kyungpook National University, 386, Gajang-dong, Sang-Ju, South Korea.
authors.forename: Deok-Jin
authors.initials: DJ
authors.lastname: Jang
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Chae-Seok
authors.initials: CS
authors.lastname: Lim
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, South Korea.
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Graduate School of East-West Medical Science, Kyung Hee University, Kyungkee-do, South Korea.
authors.forename: Chulhun
authors.initials: C
authors.lastname: Kang
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, South Korea.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
authors.forename: Bong-Kiun
authors.initials: BK
authors.lastname: Kaang
mesh_terms: D000818:Animals; D000076246:CCCTC-Binding Factor; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology; D057809:HEK293 Cells; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D008810:Mice, Inbred C57BL; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism; D015398:Signal Transduction; D009435:Synaptic Transmission
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; C000619698:Ctcf protein, mouse; C000621322:LLP protein, mouse; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins