title: Acquired multi-azole resistance in Candida tropicalis during persistent urinary tract infection in a dog.
abstract: Multi-azole resistance acquisition by Candida tropicalis after prolonged antifungal therapy in a dog with urinary candidiasis is reported. Pre- and post-azole treatment isolates were clonally related and had identical silent mutations in the ERG11 gene, but the latter displayed increased azole minimum inhibitory concentrations. A novel frameshift mutation in ERG3 was found in some isolates recovered after resistance development, so it appears unlikely that this mutation is responsible for multi-azole resistance.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Álvarez-Pérez
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28040, Spain; Hospital Clínico Veterinario Complutense, Madrid 28040, Spain.
authors.forename: Marta E
authors.initials: ME
authors.lastname: García
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28040, Spain; Hospital Clínico Veterinario Complutense, Madrid 28040, Spain.
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Cutuli
authors.affiliation: Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Fermín
authors.affiliation: Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Daza
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain; Departamento de Medicina, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Peláez
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid 28040, Spain; Hospital Clínico Veterinario Complutense, Madrid 28040, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Blanco
keywords: Azole resistance; Candida tropicalis; Dogs; Multilocus sequence typing; Urinary tract infections