title: Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches.
abstract: In neurons, ligand-gated ion channels decode the chemical signal of neurotransmitters into an electric response, resulting in a transient excitation or inhibition. Neurotransmitters act on multiple receptor types and subtypes, with spatially and temporally precise patterns. Hence, understanding the neural function of a given receptor requires methods for its targeted, rapid activation/inactivation in defined brain regions. To address this, we have developed a versatile optochemical genetic strategy, which allows the reversible control of defined receptor subtypes in designated cell types, with millisecond and micrometer precision. In this chapter, we describe the engineering of light-activated and -inhibited neuronal nicotinic acetylcholine receptors, as well as their characterization and use in cultured cells.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UM 119, 9 Quai St Bernard, 75005, Paris, France.|Neuroscience Paris Seine, CNRS, UMR 8246, 75005, Paris, France.|Neuroscience Paris Seine, INSERM, U1130, 75005, Paris, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Lemoine
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UM 119, 9 Quai St Bernard, 75005, Paris, France.|Neuroscience Paris Seine, CNRS, UMR 8246, 75005, Paris, France.|Neuroscience Paris Seine, INSERM, U1130, 75005, Paris, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Durand-de Cuttoli
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, UM 119, 9 Quai St Bernard, 75005, Paris, France. alexandre.mourot@inserm.fr.|Neuroscience Paris Seine, CNRS, UMR 8246, 75005, Paris, France. alexandre.mourot@inserm.fr.|Neuroscience Paris Seine, INSERM, U1130, 75005, Paris, France. alexandre.mourot@inserm.fr.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Mourot
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D009474:Neurons / Q000378:metabolism* / Q000528:radiation effects; D062308:Optogenetics / Q000379:methods*; D018408:Patch-Clamp Techniques / Q000379:methods; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014162:Transfection / Q000379:methods; D014981:Xenopus
chemical_list: D012333:RNA, Messenger; D011978:Receptors, Nicotinic
keywords: Chemical–optogenetics; Ligand-gated ion channels; Nicotinic acetylcholine receptor; Optogenetic pharmacology; Optogenetics; Photoswitches; Receptor