title: Facilitation of bone resorption activities in synovial lavage fluid patients with mandibular condyle fractures.
abstract: The aim of this study was to investigate the bone resorption effect of the mediators delivered in joint cavity of patients with mandibular condyle fractures by detecting osteoclast markers using cellular biochemistry methods, and by analysing bone resorption activities via inducing osteoclast differentiation of the infiltrated cells from arthrocentesis. Sixteen joints in 10 patients with mandibular condyle fractures were evaluated. The control group consisted of synovial fluid (SF) samples from seven joints of four volunteers who had no clinical signs or symptoms involving the temporomandibular joint (TMJ) or disc displacement. We collected SF cells from all patients during therapeutic arthrocentesis. The infiltrating cells from TMJ SF were cultured, differentiated into tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells and examined bone resorption activities. We also investigated factors related to osteoclast induction of SF, using ELISA procedures. Osteoclast-like cells were induced from the SF cells obtained from all patients with condylar fractures. These multinucleated giant cells were positive for TRAP and actin, and had the ability to absorb dentin slices. The levels of macrophage colony-stimulating factor (M-CSF), prostaglandin E2 (PGE2), soluble form of receptor activator of nuclear factor kappa-B ligand (sRANKL) and osteoprotegerin (OPG), in SF samples from the patients, were significantly higher than in the controls. These findings indicate that bone resorption activities in SF from patients with mandibular condyle fractures were upregulated and may participate in the pathogenesis and wound healing.
authors.affiliation: Department of Dentistry and Oral Surgery, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takano
authors.affiliation: Division of Maxillofacial Surgery, Department of Oral Medicine and Surgery, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Dentistry and Oral Surgery, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nakata
authors.affiliation: Division of Maxillofacial Surgery, Department of Oral Medicine and Surgery, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nogami
authors.affiliation: Department of Dentistry, Oral and Maxillofacial-Plastic and Reconstructive Surgery, School of Medicine, Faculty of Medicine Yamagata University, Yamagata, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yusa
authors.affiliation: Department of Dentistry and Oral Surgery, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kuwajima
authors.affiliation: Department of Dentistry and Oral Surgery, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
authors.affiliation: Department of Dentistry and Oral Surgery, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001862:Bone Resorption / Q000473:pathology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines; D005260:Female; D006801:Humans; D008297:Male; D008335:Mandibular Condyle / Q000293:injuries / Q000473:pathology*; D008337:Mandibular Fractures / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D010010:Osteoclasts / Q000378:metabolism*; D053244:Osteoprotegerin / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology*; D013704:Temporomandibular Joint / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; D053244:Osteoprotegerin; D053245:RANK Ligand; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; C025940:bone resorption factor
keywords: arthrocentesis; joint-infiltrating cells; mandibular condyle fractures; osteoclast; synovial fluid; temporomandibular joint