title: Amplexicaule A exerts anti-tumor effects by inducing apoptosis in human breast cancer.
abstract: Chemotherapy is the main treatment for patients with breast cancer metastases, but natural alternatives have been receiving attention for their potential as novel anti-tumor reagents. Amplexicaule A (APA) is a flavonoid glucoside isolated from rhizomes of Polygonum amplexicaule D. Don var. sinense Forb (PADF). We found that APA has anti-tumor effects in a breast cancer xenograft mouse model and induces apoptosis in breast cancer cell lines. APA increased levels of cleaved caspase-3,-8,-9 and PARP, which resulted from suppression of MCL-1 and BCL-2 expression in the cells. APA also inactivated the Akt/mTOR pathway in breast cancer cells. Thus, APA exerts a strong anti-tumor effect on breast cancer cells, most likely through induction of apoptosis. Our study is the first to identify this novel anti-tumor compound and provides a new strategy for isolation and separation of single compounds from herbs.
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan, PR China.|Pennsylvania State University College of Medicine, Department of Pediatrics, Hershey, PA, USA.
authors.forename: Meixian
authors.initials: M
authors.lastname: Xiang
authors.affiliation: Renmin Hospital of Wuhan University, Wuhan, PR China.
authors.forename: Hanwen
authors.initials: H
authors.lastname: Su
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan, PR China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Shu
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan, PR China.
authors.forename: Dingrong
authors.initials: D
authors.lastname: Wan
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.affiliation: Pennsylvania State University College of Medicine, Department of Pediatrics, Hershey, PA, USA.
authors.forename: Morgann
authors.initials: M
authors.lastname: Loaec
authors.affiliation: Pennsylvania State University College of Medicine, Department of Pediatrics, Hershey, PA, USA.
authors.forename: Yali
authors.initials: Y
authors.lastname: Ding
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Pennsylvania State University College of Medicine, Department of Pediatrics, Hershey, PA, USA.
authors.forename: Sinisa
authors.initials: S
authors.lastname: Dovat
authors.affiliation: College of Pharmacy, South-Central University for Nationalities, Wuhan, PR China.
authors.forename: Gaungzhong
authors.initials: G
authors.lastname: Yang
authors.affiliation: Pennsylvania State University College of Medicine, Department of Pediatrics, Hershey, PA, USA.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Song
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D028502:Polygonum / Q000737:chemistry; D011897:Random Allocation; D023041:Xenograft Model Antitumor Assays
chemical_list: D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts
keywords: Akt/mTOR; MCL-1; amplexicaule A; apoptosis; breast cancer