title: Microsatellites for Oenothera gayleana and O. hartwegii subsp. filifolia (Onagraceae), and their utility in section Calylophus.
abstract: OBJECTIVE
  Eleven nuclear and four plastid microsatellite markers were screened for two gypsum endemic species, Oenothera gayleana and O. hartwegii subsp. filifolia, and tested for cross-amplification in the remaining 11 taxa within Oenothera sect. Calylophus (Onagraceae).
  
  
  RESULTS
  Microsatellite markers were tested in two to three populations spanning the ranges of both O. gayleana and O. hartwegii subsp. filifolia. The nuclear microsatellite loci consisted of both di- and trinucleotide repeats with one to 17 alleles per population. Several loci showed significant deviation from Hardy-Weinberg equilibrium, which may be evidence of chromosomal rings. The plastid microsatellite markers identified one to seven haplotypes per population. The transferability of these markers was confirmed in all 11 taxa within Oenothera sect. Calylophus.
  
  
  CONCLUSIONS
  The microsatellite loci characterized here are the first developed and tested in Oenothera sect. Calylophus. These markers will be used to assess whether pollinator foraging distance influences population genetic parameters in predictable ways.
authors.affiliation: Plant Biology and Conservation, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA; Program in Plant Biology and Conservation, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208 USA.
authors.forename: Emily M
authors.initials: EM
authors.lastname: Lewis
authors.affiliation: Plant Biology and Conservation, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
authors.forename: Jeremie B
authors.initials: JB
authors.lastname: Fant
authors.affiliation: Department of Biology, Oberlin College, 119 Woodland Street, Oberlin, Ohio 44074 USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Moore
authors.affiliation: Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York 14853.
authors.forename: Amy P
authors.initials: AP
authors.lastname: Hastings
authors.affiliation: Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York 14853; Division of Biological Sciences, University of Montana, 32 Campus Drive HS104, Missoula, Montana 59812.
authors.forename: Erica L
authors.initials: EL
authors.lastname: Larson
authors.affiliation: Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York 14853.
authors.forename: Anurag A
authors.initials: AA
authors.lastname: Agrawal
authors.affiliation: Plant Biology and Conservation, Chicago Botanic Garden, 1000 Lake Cook Road, Glencoe, Illinois 60022 USA.
authors.forename: Krissa A
authors.initials: KA
authors.lastname: Skogen
keywords: Oenothera sect. Calylophus; Onagraceae; gypsum endemism; microsatellites; population genetics