title: Evidence for elevated (LIMK2 and CFL1) and suppressed (ICAM1, EZR, MAP2K2, and NOS3) gene expressions in metabolic syndrome.
abstract: The metabolic syndrome (MetS) is a common multicomponent condition including abdominal obesity, dyslipidemia, hypertension, and hyperglycaemia. The aim of this study was to investigate the associations of the expression of a panel of signalling genes with the MetS in a Turkish population. A total of 54 MetS patients and 42 healthy controls with similar age and sex were included to this study. mRNA from blood samples was extracted, and real-time polymerase chain reaction was performed for gene expressions using a BioMark 96.96 dynamic array system. We observed marked increases in LIM kinase 2 (LIMK2) and cofilin 1 (CFL1) gene expressions in MetS patients. However, there were significant decreases in intercellular adhesion molecules 1 (ICAM1), ezrin (EZR), mitogen-activated protein kinase kinase 2 (MAP2K2), and nitric oxide synthase 3 (NOS3) gene expressions in MetS patients. Additionally, no marked changes were noted in other 15 genes studied. This is the first study to provide evidence that activation of LIMK2/CFL1 pathway may play an important role in MetS.
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey. suzan2471@yahoo.com.tr.
authors.forename: Suzan
authors.initials: S
authors.lastname: Tabur
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Oztuzcu
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, Harran University, 63300, Sanliurfa, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Oguz
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey.
authors.forename: Seniz
authors.initials: S
authors.lastname: Demiryürek
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Dagli
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey.
authors.forename: Belgin
authors.initials: B
authors.lastname: Alasehirli
authors.affiliation: Division of Endocrinology, Department of Internal Medicine, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey.
authors.forename: Mesut
authors.initials: M
authors.lastname: Ozkaya
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, University of Gaziantep, 27310, Gaziantep, Turkey.
authors.forename: Abdullah T
authors.initials: AT
authors.lastname: Demiryürek
mesh_terms: D000328:Adult; D051338:Cofilin 1 / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D054461:Lim Kinases / Q000235:genetics*; D048370:MAP Kinase Kinase 2 / Q000235:genetics*; D008297:Male; D024821:Metabolic Syndrome / Q000235:genetics*; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D015854:Up-Regulation; D000099149:Ezrin
chemical_list: D051338:Cofilin 1; D003598:Cytoskeletal Proteins; D018799:Intercellular Adhesion Molecule-1; D054461:Lim Kinases; D048370:MAP Kinase Kinase 2; D052250:Nitric Oxide Synthase Type III; D000099149:Ezrin; C573420:ICAM1 protein, human; C496314:NOS3 protein, human; C102895:LIMK2 protein, human
keywords: Cell signalling; Cofilin; Gene expression; LIM kinase; Metabolic syndrome