title: Reorganisation of a mesophilic biogas microbiome as response to a stepwise increase of ammonium nitrogen induced by poultry manure supply.
abstract: An anaerobic digestion experiment was investigated to evaluate the impact of increasing amounts of ammonium nitrogen due to poultry manure addition on the reactor performance, especially on the microbiome response. The microbial community structure was assessed by using a 16S rRNA gene approach, which was further correlated with the prevalent environmental conditions by using statistical analyses. The addition of 50% poultry manure led to a process disturbance indicated by a high VFA content (almost 10 g(HAc-Eq) L(-1)) in combination with elevated concentrations of ammonium nitrogen (5.9 g NH4(+)-N kg(FM)(-1)) and free ammonia (0.5 g NH3 kg(FM)(-1)). Simultaneously the microbiome, changed from a Bacteroidetes-dominated to a Clostridiales-dominated community accompanied by a shift from the acetoclastic to the hydrogenotrophic pathway. The "new" microbial community was functional redundant as the overall process rates were similar to the former one. A further increase of poultry manure resulted in a complete process failure.
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany; Faculty of Agricultural Science, Georg-August-University Göttingen, Büsgenweg 5, D-37077 Göttingen, Germany.
authors.forename: Khulud
authors.initials: K
authors.lastname: Alsouleman
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Linke
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Klang
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Klocke
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany.
authors.forename: Niclas
authors.initials: N
authors.lastname: Krakat
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim (ATB), Department Bioengineering, Max-Eyth-Allee 100, D-14469 Potsdam, Germany. Electronic address: susanne.theuerl@googlemail.com.
authors.forename: Susanne
authors.initials: S
authors.lastname: Theuerl
mesh_terms: D000641:Ammonia / Q000378:metabolism; D064751:Ammonium Compounds / Q000378:metabolism; D000818:Animals; D041963:Bacteroidetes / Q000235:genetics / Q000502:physiology; D056804:Biofuels / Q000382:microbiology*; D000068016:Eubacteriales / Q000235:genetics / Q000502:physiology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D008372:Manure; D064307:Microbiota / Q000235:genetics / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism; D011200:Poultry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D056804:Biofuels; D005232:Fatty Acids, Volatile; D008372:Manure; D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D009584:Nitrogen
keywords: Anaerobic digestion; Disturbance; Microbial community reconstruction; NH(4)(+)-N inhibition; Poultry manure