title: Binding site of MraZ transcription factor in Mollicutes.
abstract: Mollicutes (mycoplasmas) feature a significant loss of known regulators of gene expression. Here, we identified the recognition site of the MraZ-family regulator of Mycoplasma gallisepticum, which is conserved in many species of different clades within class Mollicutes. The MraZ binding site is AAAGTG[T/G], in the promoter of mraZ gene it forms a series of direct repeats with a structure (AAAGTG[T/G]N3)k, where k = 3 most frequently. MraZ binds to a single repeat as an octamer complex. MraZ can also bind a single binding site or a series of repeats with different spacer lengths (2-4 nt); thus, it may play a role in the regulation of multiple operons in Mollicutes. In M. gallisepticum, MraZ acts as a transcriptional activator. The overexpression of MraZ leads to moderate filamentation of cells and the formation of aggregates, likely as a result of incomplete cytokinesis.
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow 119992, Russia. Electronic address: herr.romanoff@gmail.com.
authors.forename: G Y
authors.initials: GY
authors.lastname: Fisunov
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow 119992, Russia; Department of Bioinformatics and Bioengineering, Lomonosov Moscow State University, Leninskiye Gory, GSP-1, 73, Moscow 119234, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Evsyutina
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow 119992, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Semashko
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow 119992, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Arzamasov
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow 119992, Russia; Moscow Institute of Physics and Technology, Institutsky 9, Dolgoprudny 141700, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Manuvera
authors.affiliation: Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 33, Bld. 2 Leninsky Ave., Moscow 119071, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Letarov
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya 1a, Moscow 119992, Russia; Moscow Institute of Physics and Technology, Institutsky 9, Dolgoprudny 141700, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
authors.forename: V M
authors.initials: VM
authors.lastname: Govorun
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D045686:Mycoplasma gallisepticum / Q000235:genetics / Q000378:metabolism*; D009876:Operon / Q000502:physiology*; D020218:Response Elements / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors
keywords: Mollicutes; Mycoplasma; Transcription; Transcription factor