title: Development and Validation of a Reversed-Phase HPLC Method for the Quantitative Determination of Ten Polyphenols Extracted from Apple Peel.
abstract: A method based on a reversed-phase HPLC method was established, optimized, and validated for the separation and quantitation of 10 polyphenols extracted from the peel of apple species. A bidentate reversed-phase C18 column was used as stationary phase, and an acidified water buffer and methanol were used as mobile phase. The polyphenols were well separated and detected using UV at 280 and 320 nm. Validation parameters, such as linearity, LOD, LOQ, accuracy, and precision, were acceptable for all 10 polyphenols. The proposed method has enough linearity with correlation coefficient >0.99 within the investigated range for all tested polyphenols. The LOD was 0.24 μg/mL for ellagic acid and <0.2 μg/mL for all other polyphenols. The LOQ was 9.39 × 10(-2) μg/mL for chlorogenic acid, and ellagic acid, 2.82 × 10(-2) μg/mL for caffeic acid and >0.1 μg/mL for all other polyphenols. Recovery was within the acceptable range from 98.38 to 100.39% for all polyphenols standards. Satisfactory precision was achieved for both intra- and interday assay, with RSD <2%. The method was successfully applied for simultaneous analysis of polyphenols from apple peel.
authors.affiliation: Henan Institute of Science and Technology, School of Food Science, East Hualan Rd, Xinxiang City, Henan Province, People's Republic of China 453003.
authors.forename: Junjian
authors.initials: J
authors.lastname: Ran
authors.affiliation: Xinke College, Henan Institute of Science and Technology, Xinxiang, Henan, People's Republic of China 453003.
authors.forename: Huadi
authors.initials: H
authors.lastname: Sun
authors.affiliation: Northwest A&F University, College of Food Science and Engineering, Yangling, People's Republic of China 712100.
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Henan Branch of China Grain Reserves Corp., Zhengzhou, Henan, People's Republic of China 450046.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Henan Institute of Science and Technology, School of Food Science, East Hualan Rd, Xinxiang City, Henan Province, People's Republic of China 453003.
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D027845:Malus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D059808:Polyphenols