title: Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model.
abstract: OBJECTIVE
  To compare the effects of the surgical insult of cataract surgery on corneal inflammatory infiltration, neovascularization (NV) and lymphangiogenesis (LY) between the dry eye and non-dry eye in murine cataract surgery models.
  
  
  METHODS
  We established two groups of animals, one with normal eyes (non-dry eye) and the second with induced dry eyes. In both groups, we used surgical insults to mimic human cataract surgery, which consisted of lens extraction, corneal incision and suture. After harvesting of corneas on the 9(th) postoperative day and immunohistochemical staining, we compared NV, LY and CD11b+ cell infiltration in the corneas.
  
  
  RESULTS
  Dry eye group had significantly more inflammatory infiltration (21.75%±7.17% vs 3.65%±1.49%; P=0.049). The dry eye group showed significantly more NV (48.21%±4.02% vs 26.24%±6.01%; P=0.016) and greater levels of LY (9.27%±0.48% vs 4.84%±1.15%; P=0.007). In corneas on which no surgery was performed, there was no induction of NV in both the dry and non-dry group, but dry eye group demonstrated more CD11b+ cells infiltration than the non-dry eye group (0.360%±0.160% vs 0.023%±0.006%; P=0.068). Dry eye group showed more NV than non-dry eye group in both topical PBS application and subconjunctival PBS injection (P=0.020 and 0.000, respectively).
  
  
  CONCLUSIONS
  In a murine cataract surgery model, preexisting dry eye can induce more postoperative NV, LY, and inflammation in corneal tissue.
authors.affiliation: Department of Ophthalmology, St.Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon 442723, Gyeonggi-Do, Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Kwon
authors.affiliation: Department of Ophthalmology, St.Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon 442723, Gyeonggi-Do, Korea.
authors.forename: Yeon Woong
authors.initials: YW
authors.lastname: Chung
authors.affiliation: Department of Ophthalmology, St.Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon 442723, Gyeonggi-Do, Korea.
authors.forename: Jin A
authors.initials: JA
authors.lastname: Choi
authors.affiliation: Department of Ophthalmology, St.Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon 442723, Gyeonggi-Do, Korea.
authors.forename: Tae Yoon
authors.initials: TY
authors.lastname: La
authors.affiliation: Department of Ophthalmology, St.Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon 442723, Gyeonggi-Do, Korea.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Jee
authors.affiliation: Department of Ophthalmology, St.Vincent's Hospital, College of Medicine, The Catholic University of Korea, Suwon 442723, Gyeonggi-Do, Korea.
authors.forename: Yang Kyung
authors.initials: YK
authors.lastname: Cho
keywords: angiogenesis; cataract surgery; dry eye disease; inflammation