title: Support Vector Machine Based on microRNA Expression Profiles to Predict Histological Origin of Ampullary Carcinoma: Case Report of a Patient Affected From Adenocarcinoma of the Papilla of Vater With Lynch Syndrome.
abstract: Adenocarcinomas of Vater's papilla (PVAC) may originate from either the pancreatic duct or the intestinal epithelium. Conflicting data have been reported about the frequency of the 2 anatomical entities and their influence on patients' prognosis. To ascertain the anatomical origin of PVAC in a family member of a Lynch syndrome kindred, we searched for microRNA (miRNA) expression profiles on resected tumor specimens. The support vector machine was trained on our previous miRNAs expression data sets of pancreatic and colorectal tissue samples and used to classify the site of origin of the tumor in our patient. The support vector machine worked by contrasting the profiles of miRNAs in patients with pancreatic ductal and colorectal cancers to that of our patient, which was finally classified as pancreatic ductal adenocarcinoma accordingly to alterations of 55 miRNAs. The PVAC might be originated from ductal epithelium rather than from the intestinal mucosa of the papilla in the case at issue. Alteration of miR-548b-3p, miR-551a, miR-21, miR-92a, miR-let-7i, and miR-181a* emerged as potentially associated with cancer genetic susceptibility in PVAC.
authors.affiliation: From the *Division of Gastroenterology and Research Laboratory, †Unit of Biostatistics, ‡Unit of Medical Genetics, §Department of Surgery, IRCCS "Casa Sollievo della Sofferenza" Hospital, San Giovanni Rotondo (FG); and ∥Division of Surgical Oncology, "SS Annunziata" Hospital, Chieti, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Tavano
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Copetti
authors.forename: Ada
authors.initials: A
authors.lastname: Piepoli
authors.forename: Massimo
authors.initials: M
authors.lastname: Carella
authors.forename: Annamaria
authors.initials: A
authors.lastname: Gentile
authors.forename: Francesca Paola
authors.initials: FP
authors.lastname: Burbaci
authors.forename: Andrea
authors.initials: A
authors.lastname: Fontana
authors.forename: Antonio
authors.initials: A
authors.lastname: De Bonis
authors.forename: Fabio Francesco
authors.initials: FF
authors.lastname: di Mola
authors.forename: Pierluigi
authors.initials: P
authors.lastname: di Sebastiano
authors.forename: Angelo
authors.initials: A
authors.lastname: Andriulli
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D000293:Adolescent; D000328:Adult; D014670:Ampulla of Vater / Q000473:pathology*; D002277:Carcinoma / Q000235:genetics*; D002648:Child; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000235:genetics; D005192:Family Health; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D010183:Pancreatic Ducts / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics; D010375:Pedigree; D011379:Prognosis; D060388:Support Vector Machine; D055815:Young Adult
chemical_list: D035683:MicroRNAs