title: Virus-Host Interactions: From Unbiased Genetic Screens to Function.
abstract: Deciphering the many interactions that occur between a virus and host cell over the course of infection is paramount to understanding mechanisms of pathogenesis and to the future development of antiviral therapies. Over the past decade, researchers have started to understand these complicated relationships through the development of methodologies, including advances in RNA interference, proteomics, and the development of genetic tools such as haploid cell lines, allowing high-throughput screening to identify critical contact points between virus and host. These advances have produced a wealth of data regarding host factors hijacked by viruses to promote infection, as well as antiviral factors responsible for subverting viral infection. This review highlights findings from virus-host screens and discusses our thoughts on the direction of screening strategies moving forward.
authors.affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; email: hramage@mail.med.upenn.edu , cherrys@mail.med.upenn.edu.
authors.forename: Holly
authors.initials: H
authors.lastname: Ramage
authors.affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; email: hramage@mail.med.upenn.edu , cherrys@mail.med.upenn.edu.
authors.forename: Sara
authors.initials: S
authors.lastname: Cherry
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D006801:Humans; D014777:Virus Diseases / Q000235:genetics* / Q000503:physiopathology / Q000821:virology*; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000235:genetics
keywords: RNA interference; antiviral host factors; gain-of-function screens; haploid genetic screens; high-throughput screening; pro-viral host factors