title: Pathways for Modulating Exosome Lipids Identified By High-Density Lipoprotein-Like Nanoparticle Binding to Scavenger Receptor Type B-1.
abstract: Exosomes are produced by cells to mediate intercellular communication, and have been shown to perpetuate diseases, including cancer. New tools are needed to understand exosome biology, detect exosomes from specific cell types in complex biological media, and to modify exosomes. Our data demonstrate a cellular pathway whereby membrane-bound scavenger receptor type B-1 (SR-B1) in parent cells becomes incorporated into exosomes. We tailored synthetic HDL-like nanoparticles (HDL NP), high-affinity ligands for SR-B1, to carry a fluorescently labeled phospholipid. Data show SR-B1-dependent transfer of the fluorescent phospholipid from HDL NPs to exosomes. Modified exosomes are stable in serum and can be directly detected using flow cytometry. As proof-of-concept, human serum exosomes were found to express SR-B1, and HDL NPs can be used to label and isolate them. Ultimately, we discovered a natural cellular pathway and nanoparticle-receptor pair that enables exosome modulation, detection, and isolation.
authors.affiliation: Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|Simpson Querrey Institute for BioNanotechnology, Northwestern University Feinberg School of Medicine, Chicago, IL United States.|Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.
authors.forename: Nicholas L
authors.initials: NL
authors.lastname: Angeloni
authors.affiliation: Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|Simpson Querrey Institute for BioNanotechnology, Northwestern University Feinberg School of Medicine, Chicago, IL United States.|Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|Driskill Graduate Program in Life Sciences, Northwestern University, Chicago, IL, United States.
authors.forename: Kaylin M
authors.initials: KM
authors.lastname: McMahon
authors.affiliation: Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.
authors.forename: Suchitra
authors.initials: S
authors.lastname: Swaminathan
authors.affiliation: Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|Simpson Querrey Institute for BioNanotechnology, Northwestern University Feinberg School of Medicine, Chicago, IL United States.|Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|Driskill Graduate Program in Life Sciences, Northwestern University, Chicago, IL, United States.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Plebanek
authors.affiliation: The Ronald O. Perelman Department of Dermatology, New York University School of Medicine, New York, NY, United States.|Laura and Isaac Perlmutter Cancer Center, New York University, Langone Medical Center, New York, NY, United States.
authors.forename: Iman
authors.initials: I
authors.lastname: Osman
authors.affiliation: Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Volpert
authors.affiliation: Department of Urology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|Simpson Querrey Institute for BioNanotechnology, Northwestern University Feinberg School of Medicine, Chicago, IL United States.|Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, United States.|International Institute for Nanotechnology (IIN), Northwestern University, Evanston, IL, United States.
authors.forename: C Shad
authors.initials: CS
authors.lastname: Thaxton
mesh_terms: D015374:Biosensing Techniques; D002450:Cell Communication / Q000235:genetics*; D055354:Exosomes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D050356:Lipid Metabolism / Q000235:genetics; D008075:Lipoproteins, HDL / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D051122:Scavenger Receptors, Class B / Q000097:blood / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D008024:Ligands; D008075:Lipoproteins, HDL; D010743:Phospholipids; D051122:Scavenger Receptors, Class B