title: Neonatal SSRI exposure improves mitochondrial function and antioxidant defense in rat heart.
abstract: Protein restriction during prenatal, postnatal, or in both periods has a close relationship with subsequent development of cardiovascular disease in adulthood. Elevated brain levels of serotonin and its metabolites have been found in malnourished states. The aim in the present study was to investigate whether treatment with fluoxetine (Fx), a selective serotonin reuptake inhibitor, mimics the detrimental effect of low-protein diet during the perinatal period on the male rat heart. Our hypothesis is that increased circulating serotonin as a result of pharmacologic treatment with Fx leads to cardiac dysfunction similar to that observed in protein-restricted rats. Male Wistar rat pups received daily subcutaneous injection of Fx or vehicle from postnatal day 1 to postnatal day 21. Male rats were euthanized at 60 days of age and the following parameters were evaluated in the cardiac tissue: mitochondrial respiratory capacity, respiratory control ratio, reactive oxygen species (ROS) production, mitochondrial membrane potential, and biomarkers of oxidative stress and antioxidant defense. We found that Fx treatment increased mitochondrial respiratory capacity (123%) and membrane potential (212%) and decreased ROS production (55%). In addition we observed an increase in the antioxidant capacity (elevation in catalase activity (5-fold) and glutathione peroxidase (4.6-fold)). Taken together, our results suggest that Fx treatment in the developmental period positively affects the mitochondrial bioenergetics and antioxidant defense in the cardiac tissue.
authors.affiliation: a Laboratory of Biochemistry and Exercise Biochemistry, Centro Acadêmico de Vitoria (CAV)-Federal University of Pernambuco, Vitoria de Santo Antão, Brazil.|c Biochemistry and Physiology Graduate Program, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Glauber Ruda F
authors.initials: GR
authors.lastname: Braz
authors.affiliation: a Laboratory of Biochemistry and Exercise Biochemistry, Centro Acadêmico de Vitoria (CAV)-Federal University of Pernambuco, Vitoria de Santo Antão, Brazil.|c Biochemistry and Physiology Graduate Program, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Cristiane M
authors.initials: CM
authors.lastname: Freitas
authors.affiliation: a Laboratory of Biochemistry and Exercise Biochemistry, Centro Acadêmico de Vitoria (CAV)-Federal University of Pernambuco, Vitoria de Santo Antão, Brazil.|c Biochemistry and Physiology Graduate Program, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Nascimento
authors.affiliation: b Laboratory of Biochemistry and Exercise Biochemistry; CAV-Federal University of Pernambuco, Vitoria de Santo Antão, Brazil.
authors.forename: Anderson A
authors.initials: AA
authors.lastname: Pedroza
authors.affiliation: b Laboratory of Biochemistry and Exercise Biochemistry; CAV-Federal University of Pernambuco, Vitoria de Santo Antão, Brazil.|d Neuropsychiatry and Behavior Science Graduate Program, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Aline Isabel
authors.initials: AI
authors.lastname: da Silva
authors.affiliation: b Laboratory of Biochemistry and Exercise Biochemistry; CAV-Federal University of Pernambuco, Vitoria de Santo Antão, Brazil.|c Biochemistry and Physiology Graduate Program, Federal University of Pernambuco, Recife, Brazil.|d Neuropsychiatry and Behavior Science Graduate Program, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Lagranha
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000378:metabolism; D018753:Diet, Protein-Restricted; D004734:Energy Metabolism / Q000187:drug effects; D005473:Fluoxetine / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000502:physiology; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012701:Serotonin / Q000097:blood; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002374:Catalase; D005473:Fluoxetine; D005979:Glutathione Peroxidase; D017382:Reactive Oxygen Species; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D013482:Superoxide Dismutase
keywords: antioxidant defense; cœur; défense antioxydante; heart; mitochondria; mitochondrie; serotonin; sérotonine