title: Metabolite profiling of licorice (Glycyrrhiza glabra) from different locations using comprehensive two-dimensional liquid chromatography coupled to diode array and tandem mass spectrometry detection.
abstract: Profiling of the main metabolites from several licorice (Glycyrrhiza glabra) samples collected at different locations is carried out in this work by using comprehensive two-dimensional liquid chromatography (LC × LC) coupled to diode array (DAD) and mass spectrometry (MS) detectors. The optimized method was based on the application of a HILIC-based separation in the first dimension combined with fast RP-based second dimension separation. This set-up was shown to possess powerful separation capabilities allowing separating as much as 89 different metabolites in a single sample. Identification and grouping of metabolites according to their chemical class were achieved using the DAD, MS and MS/MS data. Triterpene saponins were the most abundant metabolites followed by glycosylated flavanones and chalcones, whereas glycyrrhizic acid, as expected, was confirmed as the main component in all the studied samples. LC × LC-DAD-MS/MS was able to resolve these complex licorice samples providing with specific metabolite profiles to the different licorice samples depending on their geographical origin. Namely, from 19 to 50 specific compounds were exclusively determined in the 2D-chromatograms from the different licorice samples depending on their geographical origin, which can be used as a typical pattern that could potentially be related to their geographical location and authentication.
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain; Laboratory of Food Chemistry, Dipartimento di Agraria (QuaSic.A.Tec.), Università Mediterranea di Reggio Calabria, Reggio Calabria, loc. Feo di Vito, 89122 Reggio Calabria, Italy.
authors.forename: Lidia
authors.initials: L
authors.lastname: Montero
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Ibáñez
authors.affiliation: Laboratory of Food Chemistry, Dipartimento di Agraria (QuaSic.A.Tec.), Università Mediterranea di Reggio Calabria, Reggio Calabria, loc. Feo di Vito, 89122 Reggio Calabria, Italy.
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Russo
authors.affiliation: Laboratory of Food Chemistry, Dipartimento di Agraria (QuaSic.A.Tec.), Università Mediterranea di Reggio Calabria, Reggio Calabria, loc. Feo di Vito, 89122 Reggio Calabria, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: di Sanzo
authors.affiliation: Dipartimento di Farmacia, Università di Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Rastrelli
authors.affiliation: Dipartimento di Farmacia, Università di Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy.
authors.forename: Anna Lisa
authors.initials: AL
authors.lastname: Piccinelli
authors.affiliation: Dipartimento di Farmacia, Università di Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Celano
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cifuentes
authors.affiliation: Laboratory of Foodomics, Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain. Electronic address: m.herrero@csic.es.
authors.forename: Miguel
authors.initials: M
authors.lastname: Herrero
mesh_terms: D047188:Chalcones / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000378:metabolism; D006031:Glycosylation; D006035:Glycyrrhiza / Q000378:metabolism*; D012503:Saponins / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000378:metabolism
chemical_list: D047188:Chalcones; D005419:Flavonoids; D012503:Saponins; D014315:Triterpenes
keywords: Authentication; LC × LC; Licorice; Metabolite; Phenolic compounds; Triterpene saponins