title: Plasmodium vivax Tryptophan Rich Antigen PvTRAg36.6 Interacts with PvETRAMP and PvTRAg56.6 Interacts with PvMSP7 during Erythrocytic Stages of the Parasite.
abstract: Plasmodium vivax is most wide spread and a neglected malaria parasite. There is a lack of information on parasite biology of this species. Genome of this parasite encodes for the largest number of tryptophan-rich proteins belonging to 'Pv-fam-a' family and some of them are potential drug/vaccine targets but their functional role(s) largely remains unexplored. Using bacterial and yeast two hybrid systems, we have identified the interacting partners for two of the P. vivax tryptophan-rich antigens called PvTRAg36.6 and PvTRAg56.2. The PvTRAg36.6 interacts with early transcribed membrane protein (ETRAMP) of P.vivax. It is apically localized in merozoites but in early stages it is seen in parasite periphery suggesting its likely involvement in parasitophorous vacuole membrane (PVM) development or maintenance. On the other hand, PvTRAg56.2 interacts with P.vivax merozoite surface protein7 (PvMSP7) and is localized on merozoite surface. Co-localization of PvTRAg56.2 with PvMSP1 and its molecular interaction with PvMSP7 probably suggest that, PvTRAg56.2 is part of MSP-complex, and might assist or stabilize the protein complex at the merozoite surface. In conclusion, the PvTRAg proteins have different sub cellular localizations and specific associated functions during intra-erythrocytic developmental cycle.
authors.affiliation: Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Kriti
authors.initials: K
authors.lastname: Tyagi
authors.affiliation: Malaria group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
authors.forename: Mohammad Enayet
authors.initials: ME
authors.lastname: Hossain
authors.affiliation: Malaria group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Thakur
authors.affiliation: Department of Emergency Medicine, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Aggarwal
authors.affiliation: Malaria group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
authors.forename: Pawan
authors.initials: P
authors.lastname: Malhotra
authors.affiliation: Malaria group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
authors.forename: Asif
authors.initials: A
authors.lastname: Mohmmed
authors.affiliation: Department of Biotechnology, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Yagya Dutta
authors.initials: YD
authors.lastname: Sharma
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology*; D015723:Gene Library; D052940:Merozoites / Q000378:metabolism; D019151:Peptide Library; D010966:Plasmodium vivax / Q000502:physiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D021381:Protein Transport; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D000953:Antigens, Protozoan; D002352:Carrier Proteins; D019151:Peptide Library; D015800:Protozoan Proteins