title: Intracellular Trafficking of AAV5 Vectors.
abstract: The present study was designed to visualize the cellular trafficking of adeno-associated virus (AAV) vectors in general and AAV5 serotype vectors in particular. We fluorescently labeled AAV5 wild-type and a mutant (S652A) virus and studied their infection process by live cell imaging and confocal microscopy. Our data demonstrate considerable difference in the ability of these vectors to reach the nuclear compartment within the first 6 hr after infection.
authors.affiliation: 1 Department of Hematology, Christian Medical College and Hospital , Vellore, Tamil Nadu, India.|2 Department of Biological Sciences and Bioengineering, Indian Institute of Technology , Kanpur, Uttar Pradesh, India .
authors.forename: Balaji
authors.initials: B
authors.lastname: Balakrishnan
authors.affiliation: 1 Department of Hematology, Christian Medical College and Hospital , Vellore, Tamil Nadu, India.|2 Department of Biological Sciences and Bioengineering, Indian Institute of Technology , Kanpur, Uttar Pradesh, India .
authors.forename: Giridhara R
authors.initials: GR
authors.lastname: Jayandharan
mesh_terms: D002232:Carbocyanines / Q000008:administration & dosage; D003593:Cytoplasm / Q000378:metabolism; D000229:Dependovirus; D005456:Fluorescent Dyes / Q000008:administration & dosage; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D057900:Transcytosis
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3