title: Proteome analysis of Pueraria mirifica tubers collected in different seasons.
abstract: Pueraria mirifica-derived tuberous powder has been long-term consumed in Thailand as female hormone-replacement traditional remedies. The protein profiles of tubers collected in different seasons were evaluated. Phenol extraction, 2D-PAGE, and mass spectrometry were employed for tuberous proteome analysis. Out of the 322 proteins detected, over 59% were functionally classified as being involved in metabolism. The rest proteins were involved in defense, protein synthesis, cell structure, transportation, stress, storage, and also unidentified function. The proteins were found to be differentially expressed with respect to harvest season. Importantly, chalcone isomerase, isoflavone synthase, cytochrome p450, UDP-glycosyltransferase, and isoflavone reductase, which are all involved in the biosynthesis pathway of bioactive isoflavonoids, were most abundantly expressed in the summer-collected tubers. This is the first report on the proteomic patterns in P. mirifica tubers in relevant with seasonal variation. The study enlights the understanding of variance isoflavonoid production in P. mirifica tubers.
authors.affiliation: a Faculty of Science, Program in Biotechnology , Chulalongkorn University , Bangkok , Thailand.
authors.forename: Jutarmas
authors.initials: J
authors.lastname: Jungsukcharoen
authors.affiliation: b Laboratory of Biochemistry , Chulabhorn Research Institute , Bangkok , Thailand.
authors.forename: Daranee
authors.initials: D
authors.lastname: Chokchaichamnankit
authors.affiliation: b Laboratory of Biochemistry , Chulabhorn Research Institute , Bangkok , Thailand.
authors.forename: Chantragan
authors.initials: C
authors.lastname: Srisomsap
authors.affiliation: c Faculty of Science, Department of Biology , Chulalongkorn University , Bangkok , Thailand.
authors.forename: Wichai
authors.initials: W
authors.lastname: Cherdshewasart
authors.affiliation: d Faculty of Science, Department of Chemistry , Chulalongkorn University , Bangkok , Thailand.
authors.forename: Polkit
authors.initials: P
authors.lastname: Sangvanich
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000096:biosynthesis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D014453:Glucuronosyltransferase / Q000235:genetics / Q000378:metabolism; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism; D059625:Liquid-Liquid Extraction; D058977:Molecular Sequence Annotation; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D019800:Phenol / Q000737:chemistry; D048789:Phytoestrogens / Q000302:isolation & purification* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D035281:Plant Tubers / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D029908:Pueraria / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012621:Seasons
chemical_list: D005419:Flavonoids; D048789:Phytoestrogens; D010940:Plant Proteins; D020543:Proteome; D019800:Phenol; D003577:Cytochrome P-450 Enzyme System; C071072:vestitone reductase; D010105:Oxygenases; C048489:isoflavone synthase; D044925:Oxidoreductases Acting on CH-CH Group Donors; D014453:Glucuronosyltransferase; D019753:Intramolecular Lyases; C019865:chalcone isomerase
keywords: Isoflavonoid producing enzymes; Pueraria mirifica; plant proteomics; secondary metabolite