title: Rapamycin Inhibits Expression of Elongation of Very-long-chain Fatty Acids 1 and Synthesis of Docosahexaenoic Acid in Bovine Mammary Epithelial Cells.
abstract: Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell growth and metabolism and is sufficient to induce specific metabolic processes, includingde novolipid biosynthesis. Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene and the product of which was thought to be associated with elongation of carbon (C) chain in fatty acids. In the present study, we examined the effects of rapamycin, a specific inhibitor of mTORC1, on ELOVL1 expression and docosahexaenoic acid (DHA, C22:6 n-3) synthesis in bovine mammary epithelial cells (BMECs). We found that rapamycin decreased the relative abundance ofELOVL1mRNA, ELOVL1 expression and the level of DHA in a time-dependent manner. These data indicate that ELOVL1 expression and DHA synthesis are regulated by mTORC1 in BMECs.
authors.affiliation: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Guo
authors.affiliation: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Chaogetu
authors.initials: C
authors.lastname: Bao
authors.affiliation: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Qiburi
authors.initials: Q
authors.lastname: He
authors.affiliation: College of Basic Medical Science, Inner Mongolia Medical University, Hohhot 010110, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Bao
authors.affiliation: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Hao
authors.affiliation: College of Life Science, Inner Mongolia University, Hohhot 010021, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
keywords: Bovine Mammary Epithelial Cells [BMECs]; DHA; Elongation of Very-long-chain Fatty Acids 1 [ELOVL1]; Mammalian Target of Rapamycin Complex 1 [mTORC1]