title: Bile acids cycle disruption in patients with nasopharyngeal carcinoma promotes the elevation of interleukin-10 secretion.
abstract: BACKGROUND
  Unclear pathogenesis existed for nasopharyngeal carcinoma.
  
  
  OBJECTIVE
  to analyze the role of bile acids in the pathogenesis of nasopharyngeal carcinoma.
  
  
  METHODS
  20 healthy volunteers and 20 patients with nasopharyngeal carcinoma were enrolled between January 1(st), 2013 and December 31(st), 2014. ESI-QTOF-MS analysis of serum was performed to find altered bile acids components. The biological function of changed bile acids was investigated using in vitro experiment.
  
  
  RESULTS
  Compared with healthy volunteers, the level of DCA and GDCA exhibited higher abundance in patients with nasopharyngeal carcinoma (p<0.01). Furthermore, the biological function was investigated for the inhibition of DCA and GDCA towards the secretion of IL-10 by CD4+CD25- T cells. Both DCA and GDCA significantly inhibited the secretion of IL-10 by CD4+CD25- T cells. Furthermore, DCA+GDCA can show stronger inhibition towards the secretion of IL-10 than DCA and GDCA.
  
  
  CONCLUSIONS
  The inhibition of IL-10 secretion by elevated DCA and GDCA components in nasopharyngeal carcinoma patients is the inducer for nasopharyngeal carcinoma.
authors.affiliation: Department of Radiotherapy, Lianshui People's Hospital, Huai'an, Jiangsu, 223400, China.
authors.forename: Cheng-Shi
authors.initials: CS
authors.lastname: Wang
authors.affiliation: Department of otorhinolaryngology,Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, 223300, China.
authors.forename: Shou-Hou
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Department of otorhinolaryngology,Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, 223300, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of otorhinolaryngology,Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, 223300, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of otorhinolaryngology,Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, 223300, China.
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001647:Bile Acids and Salts / Q000097:blood*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002277:Carcinoma; D016022:Case-Control Studies; D003840:Deoxycholic Acid / Q000097:blood; D005260:Female; D006002:Glycodeoxycholic Acid / Q000097:blood; D006801:Humans; D016753:Interleukin-10 / Q000097:blood* / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000276:immunology / Q000473:pathology*
chemical_list: D001647:Bile Acids and Salts; C508609:IL10 protein, human; D003840:Deoxycholic Acid; D016753:Interleukin-10; D006002:Glycodeoxycholic Acid
keywords: T cell; bile acids; interleukin-10 (IL-10); nasopharyngeal carcinoma; pathogenesis