title: Isoorientin Prevents Hyperlipidemia and Liver Injury by Regulating Lipid Metabolism, Antioxidant Capability, and Inflammatory Cytokine Release in High-Fructose-Fed Mice.
abstract: Isoorientin (ISO), a natural flavonoid, has been found to have multiple biological properties. In the present study, obese mice with high-fructose (HF)-induced liver injury were used to investigate the hepatoprotective effects of ISO. The results showed that ISO significantly reduced the serum lipid parameters in mice fed 20% HF water. Meanwhile, ISO appeared to alleviate HF-induced lipid metabolic disorders by increasing the serum levels of apo-A1 and decreasing the serum apoB levels, apoB/apo-A1 ratio, and FAS activity in the liver. ISO also remarkably ameliorated HF-induced hepatic oxidative injury and inflammation by decreasing ALT, AST, and ALP levels; enhancing antioxidant enzyme activities; and inhibiting inflammatory cytokine (TNF-α, IL-1, IL-6) release. Histopathology of liver stained by H&E and Oil Red O showed the liver steatosis and oxidative injury after HF treatment and the protective effect of ISO. Furthermore, aortic pathology observation found that ISO had a protective effect on the vascular endothelium. This is the first report that ISO efficiently inhibited HF-induced hyperlipidemia and liver injury by ameliorating lipid metabolism, enhancing the antioxidant defensedefense system, and regulating the secretion of inflammatory cytokines.
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University , Xi'an 710119, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University , Xi'an 710119, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University , Xi'an 710119, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University , Xi'an 710119, China.
authors.forename: Daoyuan
authors.initials: D
authors.lastname: Ren
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University , Xi'an 710119, China.
authors.forename: Xingbin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D005234:Fatty Liver / Q000378:metabolism; D005632:Fructose / Q000032:analysis / Q000378:metabolism; D006949:Hyperlipidemias / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy*; D007328:Insulin / Q000097:blood; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000378:metabolism*; D047311:Luteolin / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016207:Cytokines; D007328:Insulin; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005632:Fructose; C057912:homoorientin; D047311:Luteolin
keywords: hepatic inflammation; hyperglycemia; isoorientin; lipid metabolism; liver damage; oxidative stress