title: Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs.
abstract: Bioengineering of functional lung tissue by using whole lung scaffolds has been proposed as a potential alternative for patients awaiting lung transplant. Previous studies have demonstrated that vascular resistance (Rv) could be altered to optimize the process of obtaining suitable lung scaffolds. Therefore, this work was aimed at determining how lung inflation (tracheal pressure) and perfusion (pulmonary arterial pressure) affect vascular resistance. This study was carried out using the lungs excised from 5 healthy male Sprague-Dawley rats. The trachea was cannulated and connected to a continuous positive airway pressure (CPAP) device to provide a tracheal pressure ranging from 0 to 15cmH2O. The pulmonary artery was cannulated and connected to a controlled perfusion system with continuous pressure (gravimetric level) ranging from 5 to 30cmH2O. Effective Rv was calculated by ratio of pulmonary artery pressure (PPA) by pulmonary artery flow (V'PA). Rv in the decellularized lungs scaffolds decreased at increasing V'PA, stabilizing at a pulmonary arterial pressure greater than 20cmH2O. On the other hand, CPAP had no influence on vascular resistance in the lung scaffolds after being subjected to pulmonary artery pressure of 5cmH2O. In conclusion, compared to positive airway pressure, arterial lung pressure markedly influences the mechanics of vascular resistance in decellularized lungs.
authors.affiliation: Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain; Master׳s and Doctoral Degree Programs in Rehabilitation Sciences, Nove de Julho University, Sao Paulo, Brazil.
authors.forename: Renata Kelly
authors.initials: RK
authors.lastname: da Palma
authors.affiliation: Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain; Master׳s and Doctoral Degree Programs in Rehabilitation Sciences, Nove de Julho University, Sao Paulo, Brazil.
authors.forename: Paula Naomi
authors.initials: PN
authors.lastname: Nonaka
authors.affiliation: Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain; Institut de Bioenginyeria de Catalunya, Barcelona, Spain.
authors.forename: Noelia
authors.initials: N
authors.lastname: Campillo
authors.affiliation: Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain; CIBER Enfermedades Respiratorias, Madrid, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Uriarte
authors.affiliation: Master׳s and Doctoral Degree Programs in Rehabilitation Sciences, Nove de Julho University, Sao Paulo, Brazil.
authors.forename: Jessica Julioti
authors.initials: JJ
authors.lastname: Urbano
authors.affiliation: Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain; Institut de Bioenginyeria de Catalunya, Barcelona, Spain; CIBER Enfermedades Respiratorias, Madrid, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Navajas
authors.affiliation: Unitat Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain; CIBER Enfermedades Respiratorias, Madrid, Spain; Institut Investigacions Biomediques August PiSunyer, Barcelona, Spain.
authors.forename: Ramon
authors.initials: R
authors.lastname: Farré
authors.affiliation: Master׳s and Doctoral Degree Programs in Rehabilitation Sciences, Nove de Julho University, Sao Paulo, Brazil. Electronic address: oliveira.lvf@uninove.br.
authors.forename: Luis V F
authors.initials: LVF
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D045422:Continuous Positive Airway Pressure; D007327:Insufflation; D008168:Lung / Q000098:blood supply / Q000502:physiology*; D008297:Male; D010477:Perfusion; D011651:Pulmonary Artery / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds; D014132:Trachea; D014655:Vascular Resistance
keywords: Decellularized lung; Scaffolds; Vascular resistance