title: Validation of Single Nucleotide Polymorphisms Associated with Carcass Traits in a Commercial Hanwoo Population.
abstract: Four carcass traits, namely carcass weight (CW), eye muscle area (EMA), back fat thickness (BF), and marbling score (MS), are the main price decision parameters used for purchasing Hanwoo beef. The development of DNA markers for these carcass traits for use in a beef management system could result in substantial profit for beef producers in Korea. The objective of this study was to validate the association of highly significant single nucleotide polymorphisms (SNPs) identified in a previous genome-wide association study (GWAS) with the four carcass traits in a commercial Hanwoo population. We genotyped 83 SNPs distributed across all 29 autosomes in 867 steers from a Korean Hanwoo feedlot. Six SNPs, namely ARS-BFGL-NGS-22774 (Chr4, Pos:4889229), ARS-BFGL-NGS-100046 (Chr6, Pos:61917424), ARS-BFGL-NGS-39006 (Chr27, Pos:38059196), ARS-BFGL-NGS-18790 (Chr10, Pos:26489109), ARS-BFGL-NGS-43879 (Chr9, Pos:39964297), and BTB-00775794 (Chr20, Pos:20476265), were found to be associated with CW, EMA, BF, and MS. The ARS-BFGL-NGS-22774, BTB-00775794, and ARS-BFGL-NGS-39006 markers accounted for 1.80%, 1.72%, and 1.35% (p<0.01), respectively, of the phenotypic variance in the commercial Hanwoo population. Many genes located in close proximity to the significant SNPs identified in this study were previously reported to have roles in carcass traits. The results of this study could be useful for marker-assisted selection programs.
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Daejeon 305-764, Korea.|Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, Jakarta Selatan 12540, Indonesia.
authors.forename: Pita
authors.initials: P
authors.lastname: Sudrajad
authors.affiliation: Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Jeonju 565-851, Korea.
authors.forename: Aditi
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 232-950, Korea.
authors.forename: Chang Gwon
authors.initials: CG
authors.lastname: Dang
authors.affiliation: School of Biotechnology, Yeungnam University, Daegu 717-749, Korea.
authors.forename: Jong Joo
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Department of Animal Science, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Kwan Suk
authors.initials: KS
authors.lastname: Kim
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Jun Heon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 232-950, Korea.
authors.forename: Sidong
authors.initials: S
authors.lastname: Kim
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
keywords: Carcass Traits; Hanwoo; Single Nucleotide Polymorphism; Validation