title: Characterization of wheat - Psathyrostachys huashanica small segment translocation line with enhanced kernels per spike and stripe rust resistance.
abstract: Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs), a distant wild relative of common wheat, possesses rich potentially valuable traits, such as disease resistance and more spikelets and kernels per spike, that could be useful for wheat genetic improvement. Development of wheat - P. huashanica translocation lines will facilitate its practical utilization in wheat breeding. In the present study, a wheat - P. huashanica small segmental translocation line, K-13-835-3, was isolated and characterized from the BC1F5 population of a cross between wheat - P. huashanica amphiploid PHW-SA and wheat cultivar CN16. Cytological studies showed that the mean chromosome configuration of K-13-835-3 at meiosis was 2n = 42 = 0.10 I + 19.43 II (ring) + 1.52 II (rod). GISH analyses indicated that chromosome composition of K-13-835-3 included 40 wheat chromosomes and a pair of wheat - P. huashanica translocation chromosomes. FISH results demonstrated that the small segment from an unidentified P. huashanica chromosome was translocated into wheat chromosome arm 5DS, proximal to the centromere region of 5DS. Compared with the cultivar wheat parent CN16, K-13-835-3 was highly resistant to stripe rust pathogens prevalent in China. Furthermore, spikelets and kernels per spike in K-13-835-3 were significantly higher than those of CN16 in two growing seasons. These results suggest that the desirable genes from P. huashanica were successfully transferred into CN16 background. This translocation line could be used as novel germplasm for high-yield and, eventually, resistant cultivar breeding.
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Hou-Yang
authors.initials: HY
authors.lastname: Kang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Zhi-Juan
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Xu
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Wei-Liang
authors.initials: WL
authors.lastname: Qi
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Tang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Dai-Yan
authors.initials: DY
authors.lastname: Li
authors.affiliation: b College of Resources, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zeng
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Fan
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Sha
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Zhang
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhou
mesh_terms: D001487:Basidiomycota; D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics*; D006824:Hybridization, Genetic; D010641:Phenotype; D000069600:Plant Breeding; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D006109:Poaceae / Q000235:genetics* / Q000382:microbiology; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics* / Q000382:microbiology
keywords: Psathyrostachys huashanica; kernels per spike; lignée à translocation; nombre de grains pas épi; rouille jaune; stripe rust; translocation line