title: Differential Response and Priming Dose Effect on the Proteome of Human Fibroblast and Stem Cells Induced by Exposure to Low Doses of Ionizing Radiation.
abstract: It has been suggested that a mechanistic understanding of the cellular responses to low dose and dose rate may be valuable in reducing some of the uncertainties involved in current risk estimates for cancer- and non-cancer-related radiation effects that are inherited in the linear no-threshold hypothesis. In this study, the effects of low-dose radiation on the proteome in both human fibroblasts and stem cells were investigated. Particular emphasis was placed on examining: 1. the dose-response relationships for the differential expression of proteins in the low-dose range (40-140 mGy) of low-linear energy transfer (LET) radiation; and 2. the effect on differential expression of proteins of a priming dose given prior to a challenge dose (adaptive response effects). These studies were performed on cultured human fibroblasts (VH10) and human adipose-derived stem cells (ADSC). The results from the VH10 cell experiments demonstrated that low-doses of low-LET radiation induced unique patterns of differentially expressed proteins for each dose investigated. In addition, a low priming radiation dose significantly changed the protein expression induced by the subsequent challenge exposure. In the ADSC the number of differentially expressed proteins was markedly less compared to VH10 cells, indicating that ADSC differ in their intrinsic response to low doses of radiation. The proteomic results are further discussed in terms of possible pathways influenced by low-dose irradiation.
authors.affiliation: a   Federal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
authors.forename: Monika
authors.initials: M
authors.lastname: Hauptmann
authors.affiliation: c   Center for Radiation Protection Research, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden;
authors.forename: Siamak
authors.initials: S
authors.lastname: Haghdoost
authors.affiliation: a   Federal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
authors.forename: Maria
authors.initials: M
authors.lastname: Gomolka
authors.affiliation: b   Helmholtz Zentrum München, German Research Center for Environmental Health, Department of Protein Science, Neuherberg, Germany;
authors.forename: Hakan
authors.initials: H
authors.lastname: Sarioglu
authors.affiliation: b   Helmholtz Zentrum München, German Research Center for Environmental Health, Department of Protein Science, Neuherberg, Germany;
authors.forename: Marius
authors.initials: M
authors.lastname: Ueffing
authors.affiliation: a   Federal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
authors.forename: Anne
authors.initials: A
authors.lastname: Dietz
authors.affiliation: a   Federal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
authors.forename: Ulrike
authors.initials: U
authors.lastname: Kulka
authors.affiliation: d   Helmholtz Zentrum München, German Research Center for Environmental Health, Department of Radiation Cytogenetics, Neuherberg, Germany; and.
authors.forename: Kristian
authors.initials: K
authors.lastname: Unger
authors.affiliation: e   Department of Physics, University of Pavia, Pavia, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Babini
authors.affiliation: c   Center for Radiation Protection Research, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden;
authors.forename: Mats
authors.initials: M
authors.lastname: Harms-Ringdahl
authors.affiliation: e   Department of Physics, University of Pavia, Pavia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ottolenghi
authors.affiliation: a   Federal Office for Radiation Protection, Department SG Radiation Protection and Health, Oberschleissheim, Germany;
authors.forename: Sabine
authors.initials: S
authors.lastname: Hornhardt
mesh_terms: D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D018499:Linear Energy Transfer; D014176:Protein Biosynthesis / Q000235:genetics / Q000528:radiation effects; D020543:Proteome / Q000235:genetics* / Q000528:radiation effects; D040901:Proteomics; D011836:Radiation Tolerance / Q000235:genetics; D011839:Radiation, Ionizing; D013234:Stem Cells / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D020543:Proteome