title: Degradation testing of Mg alloys in Dulbecco's modified eagle medium: Influence of medium sterilization.
abstract: This work studies the in vitro degradation of Mg alloys for bioabsorbable implant applications under near physiological conditions. For this purpose, the degradation behaviour of Mg alloys in Dulbecco's modified eagle medium (DMEM) which is a commonly used cell culture medium is analysed. Unfortunately, DMEM can be contaminated by microorganisms, acidifying the medium and accelerating the Mg degradation process by dissolution of protective degradation layers, such as (Mgx,Cay)(PO4)z. In this paper the influence of sterilization by applying UV-C radiation and antibiotics (penicillin/streptomycin) is analysed with two implant material candidates: Mg-Gd and Mg-Ag alloys; and pure magnesium as well as Mg-4Y-3RE as a reference.
authors.affiliation: Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg, 44, 3001 Leuven, Belgium. Electronic address: inigo.marco@mtm.kuleuven.be.
authors.forename: Iñigo
authors.initials: I
authors.lastname: Marco
authors.affiliation: Institute of Materials Research, Division Metallic Biomaterials, Helmholtz-Zentrum Geesthacht, Max-Planck-Str., 1, 21502 Geesthacht, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Feyerabend
authors.affiliation: Institute of Materials Research, Division Metallic Biomaterials, Helmholtz-Zentrum Geesthacht, Max-Planck-Str., 1, 21502 Geesthacht, Germany.
authors.forename: Regine
authors.initials: R
authors.lastname: Willumeit-Römer
authors.affiliation: Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg, 44, 3001 Leuven, Belgium.
authors.forename: Omer
authors.initials: O
authors.lastname: Van der Biest
mesh_terms: D000497:Alloys / Q000737:chemistry* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010782:Photolysis / Q000528:radiation effects; D019736:Prostheses and Implants; D014466:Ultraviolet Rays
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D003470:Culture Media; D008274:Magnesium
keywords: Contamination; DMEM; Degradation layer; Magnesium; Physiological degradation