title: Ultra-sensitive biosensor based on genetically engineered acetylcholinesterase immobilized in poly (vinyl alcohol)/Fe-Ni alloy nanocomposite for phosmet detection in olive oil.
abstract: An ultra-sensitive screen-printed biosensor was successfully developed for phosmet detection in olive oil, based on a genetically-engineered acetylcholinesterase (AChE) immobilized in a azide-unit water-pendant polyvinyl alcohol (PVA-AWP)/Fe-Ni alloy nanocomposite. Fe-Ni not only allowed amplifying the response current but also lowering the applied potential from 80 mV to 30 mV vs Ag/AgCl. The biosensor showed a very good analytical performance for phosmet detection, with a detection limit of 0.1 nM. This detection limit is lower than the allowable concentrations set by international regulations. In addition to the good reproducibility, operational and storage stability, the developed biosensor was successfully used for the determination of phosmet in olive oil samples without any laborious pre-treatment. The phosmet recovery rate was about 96% after a simple liquid-liquid extraction.
authors.affiliation: BAE: Biocapteurs-Analyss-Environnement, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France; Polymeric Materials Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, P.O. Box: 21934, Egypt.
authors.forename: A Y
authors.initials: AY
authors.lastname: El-Moghazy
authors.affiliation: Polymeric Materials Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria, P.O. Box: 21934, Egypt.
authors.forename: E A
authors.initials: EA
authors.lastname: Soliman
authors.affiliation: Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, P.O. Box 832, Alexandria, Egypt.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Ibrahim
authors.affiliation: BAE: Biocapteurs-Analyss-Environnement, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors.forename: T
authors.initials: T
authors.lastname: Noguer
authors.affiliation: BAE: Biocapteurs-Analyss-Environnement, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors.forename: J-L
authors.initials: JL
authors.lastname: Marty
authors.affiliation: BAE: Biocapteurs-Analyss-Environnement, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France.
authors.forename: G
authors.initials: G
authors.lastname: Istamboulie
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000235:genetics; D000497:Alloys / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000235:genetics; D007501:Iron / Q000737:chemistry*; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D053761:Nanocomposites / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D000069463:Olive Oil / Q000032:analysis*; D010706:Phosmet / Q000032:analysis*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000497:Alloys; D004800:Enzymes, Immobilized; D000069463:Olive Oil; C030616:ferronickel; D009532:Nickel; D011142:Polyvinyl Alcohol; D007501:Iron; D000110:Acetylcholinesterase; D010706:Phosmet
keywords: Acetylcholinesterase; Biosensor; Nanocomposite; Olive oil; Organophosphorus pesticides; Screen-printed electrodes