title: Biochemical Modulation by Carbon and Nitrogen Addition in Cultures of Dictyota menstrualis (Dictyotales, Phaeophyceae) to Generate Oil-based Bioproducts.
abstract: Dictyota menstrualis (Hoyt) Schnetter, Hörning & Weber-Peukert (Dictyotales, Phaeophyceae) was studied for the production of oil-based bioproducts and co-products. Experiments were performed to evaluate the effect of carbon dioxide (CO2) concentration, under nitrogen (NO3 (-)) limiting and saturation conditions, on growth rate (GR), photosynthesis, as well as nitrate reductase (NR), carbonic anhydrase (CA), and Rubisco activities. In addition, the biochemical composition of D. menstrualis under these conditions was estimated. GR, protein content, and N content in D. menstrualis were higher in treatments containing NO3 (-), irrespective of CO2 addition. However, when CO2 was added to medium saturated with NO3 (-), values of maximum photosynthesis, Rubisco, and NR activity, as well as total soluble carbohydrates and lipids, were increased. CA activity did not vary under the different treatments. The fatty acid profile of D. menstrualis was characterized by a high content of polyunsaturated fatty acids, especially the omega-3 fatty acids, making it a possible candidate for nutraceutical use. In addition, this species presented high GR, photosynthetic rate, and fatty acid content, highlighting its economic importance and the possibility of different biotechnological applications.
authors.affiliation: Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, Caixa Postal 26077, 05599-970, São Paulo, SP, Brazil. alinepater@usp.br.
authors.forename: Aline P
authors.initials: AP
authors.lastname: Martins
authors.affiliation: Núcleo de Pesquisa em Ficologia, Instituto de Botânica, Secretaria do Meio Ambiente do Estado de São Paulo, Av. Miguel Estéfano, 3687, 04301-012, São Paulo, SP, Brazil.
authors.forename: Nair S
authors.initials: NS
authors.lastname: Yokoya
authors.affiliation: Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, Caixa Postal 26077, 05599-970, São Paulo, SP, Brazil.
authors.forename: Pio
authors.initials: P
authors.lastname: Colepicolo
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis*; D015870:Gene Expression; D009566:Nitrates / Q000378:metabolism / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism; D000459:Phaeophyceae / Q000235:genetics / Q000378:metabolism*; D017440:Photoperiod; D010788:Photosynthesis / Q000187:drug effects* / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D015525:Fatty Acids, Omega-3; D009566:Nitrates; D002245:Carbon Dioxide; D002244:Carbon; D012273:Ribulose-Bisphosphate Carboxylase; D002256:Carbonic Anhydrases; D009584:Nitrogen
keywords: Biochemical modulation; CO2 and nitrogen; Cultivation; Dictyota menstrualis; Fatty acids; Nutraceutical