title: Converting paper mill sludge into neutral lipids by oleaginous yeast Cryptococcus vishniaccii for biodiesel production.
abstract: Paper mill sludge (PMS) was assessed as cheap renewable lignocellulosic biomass for lipid production by the oleaginous yeast Cryptococcus vishniaccii (MTCC 232). The sonicated paper mill sludge extract (PMSE) exhibited enhanced lipid yield and lipid content 7.8±0.57g/l, 53.40% in comparison to 5.5±0.8g/l, 40.44% glucose synthetic medium, respectively. The accumulated triglycerides (TAG) inside the lipid droplets (LDs) were converted to biodiesel by transesterification and thoroughly characterized using GC-MS technique. The fatty acid methyl ester (FAME) profile obtained reveals elevated content of oleic acid followed by palmitic acid, linoleic acid and stearic acid with improved oxidative stability related to biodiesel quality.
authors.affiliation: Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee (IIT R), Saharanpur Campus, Saharanpur 247001, India.
authors.forename: Farha
authors.initials: F
authors.lastname: Deeba
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Pruthi
authors.affiliation: Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee (IIT R), Saharanpur Campus, Saharanpur 247001, India. Electronic address: yuvrajnegi@gmail.com.
authors.forename: Yuvraj S
authors.initials: YS
authors.lastname: Negi
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002855:Chromatography, Thin Layer; D003454:Cryptococcus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007220:Industrial Waste; D066292:Lipid Droplets / Q000737:chemistry; D008055:Lipids / Q000096:biosynthesis*; D010209:Paper; D012722:Sewage / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D007220:Industrial Waste; D008055:Lipids; D012722:Sewage
keywords: Biodiesel; Cryptococcus vishniaccii; Nile red; Paper mill sludge; Triacylglycerides