title: BACTERIA IN CRYOPRESERVED SPERM MASS OF THE WHITE SHRIMP Penaeus vannamei.
abstract: BACKGROUND
  Cryopreservation and global trading of P. vannamei sperm will become a potential and important biotechnological tool. Nevertheless, information of the possible transfer of bacteria in cryopreserved shrimp sperm has not been registered yet.
  
  
  OBJECTIVE
  The objective of this work was to determine the type of bacteria that could be cryopreserved together with white shrimp sperm masses.
  
  
  METHODS
  Sixteen sperm masses were cryopreserved in 10% DMSO and 0.5 M trehalose and sixteen fresh sperm masses were used for bacterial analysis. Bacterial colonies were isolated and selected for sequencing.
  
  
  RESULTS
  Strains were seawater borne and facultative aerobic bacteria from the genera Bacillus, Micrococcus, Paracoccus, Ruegeria and Staphylococcus. Most of them have been related with benefits to its host. None were pathogenic for P. vannamei.
  
  
  CONCLUSIONS
  Cryopreservation implies preserving pathogenic or beneficial bacteria together with the sample. Therefore, it is possible to enhance cryopreserved samples or disperse pathogenic bacteria, which needs to be prevented.
authors.affiliation: Aquaculture Department, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, Ensenada, Mexico.
authors.forename: Karina
authors.initials: K
authors.lastname: Morales-Ueno
authors.affiliation: Aquaculture Department, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, Ensenada, Mexico. cpaniagu@cicese.mx.
authors.forename: Carmen Guadalupe
authors.initials: CG
authors.lastname: Paniagua-Chávez
authors.affiliation: Insitituto de Sanidad Acuicola, Calle de la marina S/N esquina caracoles, Fraccionamiento Ensenada, Mexico.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Vasquez-Yeomans
authors.affiliation: Aquaculture Department, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, Ensenada, Mexico.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Cáceres-Martinez
authors.affiliation: Aquaculture Department, Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, Ensenada, Mexico.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: del Rio-Portilla
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015925:Cryopreservation; D008297:Male; D033561:Penaeidae / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013094:Spermatozoa / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S