title: LIGHT-INDUCED RICE1 Regulates Light-Dependent Attachment of LEAF-TYPE FERREDOXIN-NADP+ OXIDOREDUCTASE to the Thylakoid Membrane in Rice and Arabidopsis.
abstract: LIR1 (LIGHT-INDUCED RICE1) encodes a 13-kD, chloroplast-targeted protein containing two nearly identical motifs of unknown function. LIR1 is present in the genomes of vascular plants, mosses, liverworts, and algae, but not in cyanobacteria. Using coimmunoprecipitation assays, pull-down assays, and yeast two-hybrid analyses, we showed that LIR1 interacts with LEAF-TYPE FERREDOXIN-NADP(+) OXIDOREDUCTASE (LFNR), an essential chloroplast enzyme functioning in the last step of photosynthetic linear electron transfer. LIR1 and LFNR formed high molecular weight thylakoid protein complexes with the TIC62 and TROL proteins, previously shown to anchor LFNR to the membrane. We further showed that LIR1 increases the affinity of LFNRs for TIC62 and that the rapid light-triggered degradation of the LIR1 coincides with the release of the LFNR from the thylakoid membrane. Loss of LIR1 resulted in a marked decrease in the accumulation of LFNR-containing thylakoid protein complexes without a concomitant decrease in total LFNR content. In rice (Oryza sativa), photosynthetic capacity of lir1 plants was slightly impaired, whereas no such effect was observed in Arabidopsis thaliana knockout mutants. The consequences of LIR1 deficiency in different species are discussed.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China National Engineering Technology Research Center for Slow and Controlled Release Fertilizers, Kingenta Ecological Engineering Group Co., Linyi, Shandong 276700, P.R. China.
authors.forename: Hongtao
authors.identifier: http://orcid.org/0000-0002-7323-2860
authors.initials: H
authors.lastname: Hu
authors.affiliation: College of Life Science, Shaanxi Normal University, Xi'an, Shaanxi Province 710062, P.R. China.
authors.forename: Hongyan
authors.identifier: http://orcid.org/0000-0002-4651-1047
authors.initials: H
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yuzhu
authors.identifier: http://orcid.org/0000-0002-5271-051X
authors.initials: Y
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Jiangfan
authors.identifier: http://orcid.org/0000-0001-6267-4260
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Ding
authors.affiliation: Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Magda
authors.initials: M
authors.lastname: Grabsztunowicz
authors.affiliation: Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Paula
authors.identifier: http://orcid.org/0000-0002-8728-3204
authors.initials: P
authors.lastname: Mulo
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Zhongchang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Yunrong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Chuanzao
authors.identifier: http://orcid.org/0000-0001-5126-2180
authors.initials: C
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, P.R. China xiaorong@zju.edu.cn.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Mo
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D004579:Electron Transport; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism; D008027:Light; D046912:Multiprotein Complexes; D009154:Mutation; D009249:NADP / Q000378:metabolism; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D010788:Photosynthesis; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D013045:Species Specificity; D020524:Thylakoids / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D005288:Ferredoxins; D046912:Multiprotein Complexes; D010940:Plant Proteins; D009249:NADP; D005287:Ferredoxin-NADP Reductase