title: A study on the effect of the internal exposure to (210)Po on the excretion of urinary proteins in rats.
abstract: This study was designed to assess the feasibility of a noninvasive urine specimen for the detection of proteins as indicators of internal exposure to ionizing radiation. Three groups of rats (five in each group) were intravenously injected with 1601 ± 376, 10,846 ± 591 and 48,467 ± 2812 Bq of (210)Po in citrate form. A sham-exposed control group of five rats was intravenously injected with sterile physiological saline. Daily urine samples were collected over 4 days following injection. Purification and pre-concentration of urinary proteins were carried out by ultrafiltration using a 3000 Da molecular weight cutoff membrane filter. The concentration of common urinary proteins, namely albumin, alpha-1-acid glycoprotein, immunoglobulins IgA and IgG, was measured by an enzyme-linked immunosorbent assay. Urinary excretion of albumin decreased dose-dependently (p < 0.05) 96 h post-injection relative to the control group. In contrast, no statistically significant effects were observed for other proteins tested. The dose-dependent decrease in urinary excretion of albumin observed in this study underscores the need for further research, which may lead to the discovery of new biomarkers that would reflect the changes in the primary target organs for deposition of (210)Po.
authors.affiliation: National Internal Radiation Assessment Section, Radiation Protection Bureau, Health Canada, 775 Brookfield Road, Ottawa, ON, K1A 1C1, Canada. baki.sadi@hc-sc.gc.ca.
authors.forename: Baki
authors.initials: B
authors.lastname: Sadi
authors.affiliation: National Internal Radiation Assessment Section, Radiation Protection Bureau, Health Canada, 775 Brookfield Road, Ottawa, ON, K1A 1C1, Canada.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Li
authors.affiliation: National Internal Radiation Assessment Section, Radiation Protection Bureau, Health Canada, 775 Brookfield Road, Ottawa, ON, K1A 1C1, Canada.
authors.forename: Raymond
authors.initials: R
authors.lastname: Ko
authors.affiliation: National Internal Radiation Assessment Section, Radiation Protection Bureau, Health Canada, 775 Brookfield Road, Ottawa, ON, K1A 1C1, Canada.
authors.forename: Joseph
authors.initials: J
authors.lastname: Daka
authors.affiliation: Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada.
authors.forename: Hamdi
authors.initials: H
authors.lastname: Yusuf
authors.affiliation: Canadian Nuclear Laboratories, Atomic Energy of Canada Limited, Chalk River, ON, K0J 1J0, Canada.
authors.forename: Heather
authors.initials: H
authors.lastname: Wyatt
authors.affiliation: Canadian Nuclear Laboratories, Atomic Energy of Canada Limited, Chalk River, ON, K0J 1J0, Canada.
authors.forename: Joel
authors.initials: J
authors.lastname: Surette
authors.affiliation: Canadian Nuclear Laboratories, Atomic Energy of Canada Limited, Chalk River, ON, K0J 1J0, Canada.
authors.forename: Nick
authors.initials: N
authors.lastname: Priest
authors.affiliation: Nuclear Technology Research Laboratory, Radiation Safety Research Center, Central Research Institute of Electric Power Industry (CRIEPI), 2-11-1 Iwado-kita, Komae, Tokyo, 201-8511, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamada
mesh_terms: D000581:Amidohydrolases / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000652:urine; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D011059:Polonium / Q000009:adverse effects*; D011507:Proteinuria / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D014462:Ultrafiltration
chemical_list: D015415:Biomarkers; D011059:Polonium; D000581:Amidohydrolases; C019946:creatininase
keywords: Biomarkers; Internal exposure; Polonium-210; Urinary proteins