title: Reproductive responses of male Brandt's voles (Lasiopodomys brandtii) to 6-methoxybenzoxazolinone (6-MBOA) under short photoperiod.
abstract: The plant secondary metabolite 6-methoxybenzoxazolinone (6-MBOA) can stimulate and enhance animal reproduction. This compound has been successfully detected in Leymus chinensis, which is the main diet of Brandt's voles. The aim of this study was to investigate the effect of different 6-MBOA doses on the reproductive physiology of male Brandt's voles under a short photoperiod. The results showed that 6-MBOA administration increased relative testis weight, regardless of the dose, but it had little effect on the body mass. Low and middle doses of 6-MBOA increased the concentrations of luteinizing hormone and testosterone in the serum and the mRNA levels of StAR and CYP11a1 in the testes. However, 6-MBOA did not cause any significant increase in the mRNA levels of KiSS-1, GPR54, and GnRH compared to those in the control group. The mRNA level of KiSS-1 in the arcuate nucleus (ARC) was higher than that in the anteroventral periventricular nucleus (AVPV). Collectively, our results demonstrated that the number of KiSS-1-expressing neurons located in the ARC was the highest, and that 6-MBOA, which might modulate the reproductive activity along the hypothalamic-pituitary-gonadal axis, had a dose-dependent stimulatory effect on the reproductive activity of Brandt's voles under a short photoperiod. Our study provided insights into the mechanism of 6-MBOA action and the factors influencing the onset of reproduction in Brandt's voles.
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dai
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China.
authors.forename: Lian Yu
authors.initials: LY
authors.lastname: Jiang
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China.
authors.forename: Man Hong
authors.initials: MH
authors.lastname: Ye
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China.
authors.forename: Ai Qin
authors.initials: AQ
authors.lastname: Wang
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, People's Republic of China.
authors.forename: Wan Hong
authors.initials: WH
authors.lastname: Wei
authors.affiliation: College of Bioscience and Biotechnology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, People's Republic of China. smyang@yzu.edu.cn.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, People's Republic of China. smyang@yzu.edu.cn.
authors.forename: Sheng Mei
authors.initials: SM
authors.lastname: Yang
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000502:physiology*; D001583:Benzoxazoles / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009474:Neurons / Q000187:drug effects; D017440:Photoperiod; D012098:Reproduction / Q000187:drug effects*; D013737:Testis / Q000187:drug effects; D013739:Testosterone / Q000097:blood
chemical_list: D001583:Benzoxazoles; D013739:Testosterone; C031896:6-methoxybenzoxazolinone; D007986:Luteinizing Hormone
keywords: 6-MBOA; Brandt’s vole; KiSS-1/GPR54 system; Reproductive activity