title: Osteoconductive composite graft based on bacterial synthesized hydroxyapatite nanoparticles doped with different ions: From synthesis to in vivo studies.
abstract: To repair damaged bone tissues, osteoconductive bone graft substitutes are required for enhancement of the regenerative potential of osteoblast cells. Nanostructured hydroxyapatite is a bioactive ceramic used for bone tissue engineering purposes. In this study, carbonate hydroxyapatite (cHA) and zinc-magnesium substituted hydroxyapatite (Zn-Mg-HA) nanoparticles were synthesized via biomineralization method using Enterobacter aerogenes. The structural phase composition and the morphology of the samples were analyzed using appropriate powder characterization methods. Next, a composite graft was fabricated by using polyvinyl alcohol and both cHA and Zn-Mg-HA samples. In vivo osteogenic potential of the graft was then investigated in a rabbit tibial osteotomy model. Histological, radiological and morphological studies showed that the graft was mineralized by the newly formed bone tissue without signs of inflammation or infection after 4 weeks of implantation. These histomorphometric results suggest that the fabricated graft can function as a potent osteoconductive bone tissue substitute.
authors.affiliation: Department of Biotechnology, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Mazandaran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Ahmadzadeh
authors.affiliation: Department of Biotechnology, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Mazandaran, Iran. Electronic address: f.talebnia@nit.ac.ir.
authors.forename: Farid
authors.initials: F
authors.lastname: Talebnia
authors.affiliation: Nanosystems Research Team (NRTeam), Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
authors.forename: Meisam
authors.initials: M
authors.lastname: Tabatabaei
authors.affiliation: Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ahmadzadeh
authors.affiliation: Physical Intelligence Department, Max-Planck Institute for Intelligent Systems, Stuttgart, Germany. Electronic address: mostaghaci@is.mpg.de.
authors.forename: Babak
authors.initials: B
authors.lastname: Mostaghaci
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes; D017886:Durapatite; D007477:Ions; D053758:Nanoparticles
chemical_list: D018786:Bone Substitutes; D007477:Ions; D017886:Durapatite
keywords: Biomineralization; Bone graft; Hydroxyapatite; Nanoparticles; Tissue engineering