title: Biological denitrification using poly(butanediol succinate) as electron donor.
abstract: Poly(butanediol succinate) (PBS), a biodegradable polymer, was used as both solid carbon source and biofilm carrier for biological nitrate removal process, in which PBS was filled in a packed-bed bioreactor. The denitrification performance and the microbial diversity of biofilm attached on the surface of PBS were investigated. The experimental results showed that the volumetric denitrification rate was 0.60 kg m(-3) day(-1) when NO3-N loading rate was 0.63 kg m(-3) day(-1), and the average NO2-N concentration was below 0.20 mg L(-1). The effluent pH value decreased slightly from a range of 6.98-7.87 to 6.46-7.18. The analysis of microbial community structure of biofilm by pyrosequencing method showed that Proteobacteria was the most abundant phylum (89.87 %), and β-Proteobacteria represented the most abundant class. Among the 76 identified genera, Dechloromonas (10.26 %), Alicycliphilus (9.15 %), Azospira (8.92 %), and Sinobacteraceae-uncultured (8.75 %) were the abundant genera. PBS, as a promising alternative carbon source, is a suitable solid carbon source and biofilm carrier for nitrate removal.
authors.affiliation: Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China.|State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, People's Republic of China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, People's Republic of China. wangjl@tsinghua.edu.cn.|Beijing Key Laboratory of Radioactive Wastes Treatment, Tsinghua University, Beijing, 100084, People's Republic China. wangjl@tsinghua.edu.cn.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Wang
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism*; D058440:Denitrification; D004583:Electrons; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D020560:Proteobacteria / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D019802:Succinic Acid / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002072:Butylene Glycols; D009566:Nitrates; D011108:Polymers; D019802:Succinic Acid
keywords: Nitrate; Poly(butanediol succinate); denitrification; microbial community; solid carbon source