title: Synthesis of 5'-NAD-Capped RNA.
abstract: In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5'-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA. Here we report a chemoenzymatic approach to generate 5'-NAD-capped RNA in high yields and purity under mild conditions. This approach uses unprotected 5'-monophosphate RNA synthesized either chemically or enzymatically, 5',5'-pyrophosphate bond formation by phosphorimidazolide chemistry, and an enzymatic cleanup step. Thus, 5'-NAD-modified RNA can be synthesized independent of length, structure, and nucleotide sequence.
authors.affiliation: Institute for Pharmacy and Molecular Biotechnology, Heidelberg University , 69120 Heidelberg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Höfer
authors.affiliation: Institute for Pharmacy and Molecular Biotechnology, Heidelberg University , 69120 Heidelberg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Abele
authors.affiliation: Institute for Pharmacy and Molecular Biotechnology, Heidelberg University , 69120 Heidelberg, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Schlotthauer
authors.affiliation: Institute for Pharmacy and Molecular Biotechnology, Heidelberg University , 69120 Heidelberg, Germany.
authors.forename: Andres
authors.initials: A
authors.lastname: Jäschke
mesh_terms: D009243:NAD / Q000737:chemistry*; D012315:RNA Caps / Q000138:chemical synthesis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D012315:RNA Caps; D009243:NAD