title: Tissue Distribution of Porcine FTO and Its Effect on Porcine Intramuscular Preadipocytes Proliferation and Differentiation.
abstract: The fat mass and obesity associated (FTO) gene plays an important role in adipogenesis. However, its function during porcine intramuscular preadipocyte proliferation and differentiation remains poorly understood. In this study, we prepared the antiserum against porcine FTO (pFTO), which was used to determine its subcellular localization and tissue distribution. Our data indicated that pFTO was localized predominantly in the nucleus. Real-time quantitative PCR and western blot analysis showed that pFTO was highly expressed in the lung and subcutaneous adipose tissue. Overexpression of pFTO in porcine intramuscular preadipocytes significantly promoted cell proliferation and lipid deposition. Furthermore, overexpression of pFTO in differentiating porcine intramuscular preadipocytes also significantly increased the mRNA levels of adipocyte differentiation transcription factors peroxisome proliferators-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), lipoprotein lipase (LPL) and fatty acid synthase (FAS). Our findings provide the first functional evidence to reveal a role of pFTO in porcine intramuscular preadipocyte proliferation and differentiation.
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Yanliu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, 611130, P. R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
mesh_terms: D017667:Adipocytes; D000818:Animals; D050218:Body Fat Distribution; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D011506:Proteins / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D050151:Subcutaneous Fat; D013552:Swine / Q000033:anatomy & histology / Q000235:genetics*
chemical_list: D011506:Proteins