title: AtGRP3 Is Implicated in Root Size and Aluminum Response Pathways in Arabidopsis.
abstract: AtGRP3 is a glycine-rich protein (GRP) from Arabidopsis thaliana shown to interact with the receptor-like kinase AtWAK1 in yeast, in vitro and in planta. In this work, phenotypic analyses using transgenic plants were performed in order to better characterize this GRP. Plants of two independent knockout alleles of AtGRP3 develop longer roots suggesting its involvement in root size determination. Confocal microscopy analysis showed an abnormal cell division and elongation in grp3-1 knockout mutants. Moreover, we also show that grp3-1 exhibits an enhanced Aluminum (Al) tolerance, a feature also described in AtWAK1 overexpressing plants. Together, these results implicate AtGRP3 function root size determination during development and in Al stress.
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Amanda
authors.initials: A
authors.lastname: Mangeon
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Renan
authors.initials: R
authors.lastname: Pardal
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Adriana Dias
authors.initials: AD
authors.lastname: Menezes-Salgueiro
authors.affiliation: Programa de Pós-Graduação em Botânica (PPGBot), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Guilherme Leitão
authors.initials: GL
authors.lastname: Duarte
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: de Seixas
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Fernanda P
authors.initials: FP
authors.lastname: Cruz
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Cardeal
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Magioli
authors.affiliation: Departamento de Biologia, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Felipe Klein
authors.initials: FK
authors.lastname: Ricachenevsky
authors.affiliation: Centro de Biotecnologia e Departamento de Biofísica da Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Margis
authors.affiliation: Laboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-617, Brazil.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Sachetto-Martins
mesh_terms: D000535:Aluminum / Q000633:toxicity; D017360:Arabidopsis / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D018613:Microscopy, Confocal; D018517:Plant Roots / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; C071359:GRP protein, Arabidopsis; D000535:Aluminum; D011494:Protein Kinases; C101093:WAK1 protein, Arabidopsis