title: Downregulation of selenium-binding protein 1 is associated with poor prognosis in lung squamous cell carcinoma.
abstract: BACKGROUND
  We found that selenium-binding protein 1 (SBP1) was progressively decreased in the human bronchial epithelial carcinogenic processes. Knockdown of SBP1 in immortalized human bronchial epithelial cell line 16HBE cells significantly increased the efficiency of B[a]P-induced cell transformation. However, the relationship between SBP1 expression and clinicopathological factors of patients has not been defined completely. The specific role of SBP1 in prognosis of lung squamous cell carcinoma (LSCC) is still unknown.
  
  
  METHODS
  Tissue samples from 82 patients treated by pulmonary lobectomy for LSCC were used. Immunohistochemistry and western blotting were used to detect the expressions of SBP1 protein. The relationships between the expression level of SBP1 and the clinicopathological features of patients were analyzed. Cox proportional hazard regression analysis and Kaplan-Meier method were used to perform survival analysis.
  
  
  RESULTS
  Expressions of SBP1 proteins were significantly lower in LSCC tissues than that in the corresponding normal bronchial epithelium (NBE) tissues (P = 0.000). In LSCC, The expression levels of SBP1 had not correlated with patients' age, gender, smoking state, primary tumor stages (T), TNM clinical stages, and distant metastasis (M) (P > 0.05). However, downregulation of SBP1 was significantly associated with higher lymph node metastasis and lower overall survival rate (P < 0.05). Cox regression analysis indicated low expressions of SBP1 can be an independent prognostic factor for poor overall survival in LSCC patients (P = 0.002).
  
  
  CONCLUSIONS
  Downregulation of SBP1 may play a key role in the tumorigenic process of LSCC. SBP1 may be a novel potential prognostic factor of LSCC.
authors.affiliation: School of Nursing, University of South China, 28# Changsheng Road West, Hengyang, 421001, Hunan, China. 445679540@qq.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Tan
authors.affiliation: School of Nursing, University of South China, 28# Changsheng Road West, Hengyang, 421001, Hunan, China. 254251558@qq.com.
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.affiliation: School of Nursing, University of South China, 28# Changsheng Road West, Hengyang, 421001, Hunan, China. wanyy1991@aliyun.com.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Wan
authors.affiliation: School of Medicine, University of South China, Hengyang, 421001, China. meixiangle@163.com.
authors.forename: Mei-Xiang
authors.initials: MX
authors.lastname: Li
authors.affiliation: School of Medicine, University of South China, Hengyang, 421001, China. 172140411@qq.com.
authors.forename: Si-Han
authors.initials: SH
authors.lastname: Chen
authors.affiliation: School of Nursing, University of South China, 28# Changsheng Road West, Hengyang, 421001, Hunan, China. 764971367@qq.com.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Mo
authors.affiliation: School of Nursing, University of South China, 28# Changsheng Road West, Hengyang, 421001, Hunan, China. 386212360@qq.com.
authors.forename: Qiong-Lan
authors.initials: QL
authors.lastname: Zhao
authors.affiliation: School of Nursing, University of South China, 28# Changsheng Road West, Hengyang, 421001, Hunan, China. 450392434@qq.com.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Huang
authors.affiliation: School of Nursing, University of South China, 28# Changsheng Road West, Hengyang, 421001, Hunan, China. zengguqing0123@163.com.
authors.forename: Gu-Qing
authors.initials: GQ
authors.lastname: Zeng
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000556:secondary*; D015536:Down-Regulation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D051137:Selenium-Binding Proteins / Q000378:metabolism*; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D051137:Selenium-Binding Proteins