title: Bio-oil production and removal of organic load by microalga Scenedesmus sp. using culture medium contaminated with different sugars, cheese whey and whey permeate.
abstract: The objective of this study was to evaluate the bio-oil production and the organic load removal using the microalga Scenedesmus sp. The cultivation was carried out in reactors with a total volume of 3 L and 0.7 vvm aeration, with illumination in photoperiods of 12 h light/12 h dark for 12 days. The following sugar concentrations were tested: 2.5, 5.0 and 10 g/L of glucose, lactose, fructose and galactose with 10% inoculum volume. After experiments were performed with cheese whey in natura and cheese whey permeate with different lactose concentrations (1.5, 2.5, 3.5 and 5.0 g/L). In these experiments the inoculum concentrations were 10, 15, 20 and 30% (v/v). The results showed that this microalga was effective for the production of lipids when it was cultivated in medium with cheese whey in natura with 2.5 g/L of lactose and 20% inoculum (v/v). Using cheese whey in natura at the concentration of 3.5 g/L of lactose and 30% (v/v) of inoculum obtained 77.9% of TOC removal and 38.447 mg of TOC removed/mg oil produced. It was also observed that when there is increased production of bio-oil, there is less removal of organic matter. The addition of glucose, fructose or galactose in the medium did not enhance the production of bio-oil by Scenedesmus sp. when compared to lactose, but increased the organic matter removal.
authors.affiliation: Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil. Electronic address: wesley.itb@gmail.com.
authors.forename: Wesley da Silva
authors.initials: Wda S
authors.lastname: Borges
authors.affiliation: Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil.
authors.forename: Breno Severiano Alves
authors.initials: BS
authors.lastname: Araújo
authors.affiliation: Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil.
authors.forename: Lucas Gomes
authors.initials: LG
authors.lastname: Moura
authors.affiliation: Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil.
authors.forename: Ubirajara
authors.initials: U
authors.lastname: Coutinho Filho
authors.affiliation: Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil.
authors.forename: Miriam Maria
authors.initials: MM
authors.lastname: de Resende
authors.affiliation: Faculty of Chemical Engineering, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Bloco K, Uberlândia 38400-902, Minas Gerais, Brazil.
authors.forename: Vicelma Luiz
authors.initials: VL
authors.lastname: Cardoso
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry*; D005632:Fructose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D007785:Lactose / Q000032:analysis; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000378:metabolism*; D044444:Scenedesmus / Q000378:metabolism*; D000067796:Whey / Q000737:chemistry
chemical_list: D003470:Culture Media; D008055:Lipids; D005632:Fructose; D005947:Glucose; D007785:Lactose; D005690:Galactose
keywords: Bio-oil production; Microalga; Organic load removal