title: Prevention of Epidural Fibrosis in Rats by Local Administration of Mitomycin C or Daunorubicin.
abstract: OBJECTIVE
  Epineural adhesion after peripheral nerve surgery is common. The purpose of this study was to evaluate the macroscopic and histopathologic effects of topical mitomycin C and daunorubicin on epineural scar formation.
  
  
  METHODS
  In this study, we used 15 rats (30 nerves). Two test groups and one control group were created. Sciatic nerve exposure was created bilaterally in each group (30 nerve examinations in total). In experimental group 1, cotton pads that had absorbed mitomycin C (0.5 mg/ml) were placed onto the nerves for 5 minutes while in experimental group 2, cotton pads that had absorbed daunorubicin (0.2 mg/ml) were placed onto the nerves for 5 minutes and cotton pads that had absorbed saline were applied to the control group. Eight weeks after the first surgery, surgical dissection was performed for the evaluation of neurolysis sites. Epineural adhesions were classified utilizing a numerical grading layout.
  
  
  RESULTS
  We did not find any adverse effect with topically applied mitomycin C and daunorubicin. Within the 3 groups, no significant difference was seen in skin and fascia-muscle cavity closure (p > 0.05). Macroscopically, mitomycin C and daunorubicin decreased the adhesion of sciatic nerve to adjacent structures. There was intensive epineural scar formation in the control group. Scar tissue thickness and fibroblast/fibrocyte cell number were less in the two test groups compared with the control group (p < 0.001). There was no statistical difference between the two test groups.
  
  
  CONCLUSIONS
  Epineural scar formation after peripheral nerve surgery may be reduced by using topical application of mitomycin C and daunorubicin.
authors.affiliation: Derince Hospital, Department of Neurosurgery, Kocaeli, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Vural
authors.forename: Murat
authors.initials: M
authors.lastname: Yilmaz
authors.forename: Konuralp
authors.initials: K
authors.lastname: Ilbay
authors.forename: Gül
authors.initials: G
authors.lastname: Ilbay
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002921:Cicatrix / Q000473:pathology; D003630:Daunorubicin / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D005355:Fibrosis / Q000517:prevention & control; D016685:Mitomycin / Q000494:pharmacology*; D019635:Neurosurgical Procedures / Q000009:adverse effects*; D010525:Peripheral Nerves / Q000473:pathology*; D051381:Rats; D012584:Sciatic Nerve / Q000473:pathology / Q000601:surgery; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000903:Antibiotics, Antineoplastic; D016685:Mitomycin; D003630:Daunorubicin