title: "Pop-slide" patterning: Rapid fabrication of microstructured PDMS gasket slides for biological applications.
abstract: We describe a "pop-slide" patterning approach to easily produce thin film microstructures on the surface of glass with varying feature sizes (3 μm - 250 μm) and aspect ratios (0.066 - 3) within 45 minutes. This low cost method does not require specialized equipment while allowing us to produce micro structured gasket layers for sandwich assays and could be readily applied to many biological applications.
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA-06511.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Ramji
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA-06511.
authors.forename: Nafeesa T
authors.initials: NT
authors.lastname: Khan
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA-06511.
authors.forename: Andrés
authors.initials: A
authors.lastname: Muñoz-Rojas
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA-06511.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Miller-Jensen
keywords: Cell differentiation; High throughput screening; Mechanobiology; Micropatterning; Micropillars; Microwells; Open microchannels; Proteomics; Secretomic analysis; Topographies