title: Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers.
abstract: Culture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup O153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of ∼150kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40μg/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's permeabilizing factor), may be an important SEPEC virulence factor that facilitates bacterial access to the bloodstream.
authors.affiliation: Department of Genetics, Evolution and Bioagents, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Luiz Henrique Soares
authors.initials: LH
authors.lastname: Tibo
authors.affiliation: Department of Genetics, Evolution and Bioagents, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Jéssica Wildgrube
authors.initials: JW
authors.lastname: Bertol
authors.affiliation: Department of Genetics, Evolution and Bioagents, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Robert Alvin
authors.initials: RA
authors.lastname: Bernedo-Navarro
authors.affiliation: Department of Genetics, Evolution and Bioagents, Universidade Estadual de Campinas, Campinas, SP, Brazil. Electronic address: tyano@unicamp.br.
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Yano
mesh_terms: D001427:Bacterial Toxins / Q000633:toxicity; D003603:Cytotoxins / Q000378:metabolism* / Q000633:toxicity; D017097:Electric Impedance; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D018805:Sepsis / Q000382:microbiology*; D037521:Virulence Factors
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D037521:Virulence Factors
keywords: Cytotoxin (SPF); Sepsis; Transcytosis; Transendothelial electrical resistance