title: Naringenin inhibits proliferation, migration, and invasion as well as induces apoptosis of gastric cancer SGC7901 cell line by downregulation of AKT pathway.
abstract: The preliminary anti-cancer activity of Naringenin (Nar) has been proven in several cancers. However, the therapeutic activity of Nar on gastric cancer SGC-7901 cell line is not yet well understood. The aim of the present study was to investigate the effect and mechanisms of Nar on proliferation, apoptosis, migration, and invasion of SGC-7901 cells. In this in vitro study, SGC-7901 cells were treated with Nar at serial concentrations. Our data showed that Nar efficiently inhibited SGC-7901 cell proliferation in a time- and concentration-dependent manner, as well as downregulated proliferating cell nuclear antigen (PCNA) levels in a concentration-dependent manner. Meanwhile, the cell migration and invasion also dramatically decreased after Nar incubation, and the expressions of MMP2 and MMP9 were significantly downregulated. In addition, a strong proapoptotic effect was observed in the SGC-7901 cells after Nar treatment. Apoptosis-related proteins Bax and cleaved caspase-3 were up-regulated, whereas Bcl-2 and Survivin were downregulated. After administration with Nar, we found that phosphorylation of AKT was inhibited, and this inhibitory action could be mildly enhanced by the combination treatment of Nar and AKT inhibitor LY294002. In conclusion, our study confirmed that Nar could inhibit SGC-7901cell proliferation, migration, and invasion as well as induces apoptosis, and Nar might provide a new potential therapeutic strategy for treating gastric cancer.
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, No. 12, Jian-Kang Road, Shijiazhuang, 050011, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.affiliation: Department of Gastroenterology, The Third Hospital of Shijiazhuang City, 15 Sports St., Shijiazhuang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Third Department of General Surgery, Hebei General Hospital, 348 He-Ping West St., Shijiazhuang, 050051, China.
authors.forename: Huai-Bin
authors.initials: HB
authors.lastname: Guo
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, No. 12, Jian-Kang Road, Shijiazhuang, 050011, China. li_yong_hbth@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, No. 12, Jian-Kang Road, Shijiazhuang, 050011, China.
authors.forename: Bi-Bo
authors.initials: BB
authors.lastname: Tan
authors.affiliation: Third Department of General Surgery, Hebei General Hospital, 348 He-Ping West St., Shijiazhuang, 050051, China.
authors.forename: Wan-Xing
authors.initials: WX
authors.lastname: Zhang
authors.affiliation: Third Department of General Surgery, Hebei General Hospital, 348 He-Ping West St., Shijiazhuang, 050051, China.
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Peng
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D051057:Proto-Oncogene Proteins c-akt; C005273:naringenin
keywords: Gastric cancer; Invasion; Naringenin; Proliferation; Signal transduction pathway