title: Association of Complement C5 Gene Polymorphisms with Proliferative Diabetic Retinopathy of Type 2 Diabetes in a Chinese Han Population.
abstract: OBJECTIVE
  To investigate the association of C5 SNPs with proliferative diabetic retinopathy (PDR) of type 2 diabetes (T2D).
  
  
  METHODS
  A total of four C5 SNPs including rs2269067, rs7040033, rs1017119 and rs7027797 were genotyped in 400 PDR patients with T2D (cases) and 600 non- proliferative diabetic retinopathy PDR (NPDR) with T2D patients (controls) by using PCR-RFLP method. mRNA expression was examined by real-time PCR. Cytokine production was detected by ELISA.
  
  
  RESULTS
  The frequency of GG genotype of C5 rs2269067 was significantly increased in cases compared with controls (Pc = 3.4 × 10(-5), OR = 1.87). And C5 mRNA expression was significantly increased in rs2269067 GG cases as compared with CG or CC cases (P = 0.003, P = 0.001, respectively). Moreover, the production of IL-6 was significantly increased in rs2269067 GG cases compared to CG cases or CC cases (P = 0.002, P = 0.001, respectively).
  
  
  CONCLUSIONS
  C5 rs2269067 GG genotype confers risk for PDR of T2D in Chinese han population and is associated with an elevated C5 mRNA expression and an increased IL-6 production.
authors.affiliation: Chongqing General Hospital, Chongqing EYE and ENT Hospital, Chongqing, P R China.
authors.forename: Dengfeng
authors.initials: D
authors.lastname: Xu
authors.affiliation: Chongqing General Hospital, Chongqing EYE and ENT Hospital, Chongqing, P R China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yi
authors.affiliation: Chongqing General Hospital, Chongqing EYE and ENT Hospital, Chongqing, P R China.
authors.forename: Shizhi
authors.initials: S
authors.lastname: Yu
authors.affiliation: Chongqing Center for Clinical Laboratory, Chongqing Municipal People's Hospital, Chongqing, P R China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Li
authors.affiliation: Chongqing General Hospital, Chongqing EYE and ENT Hospital, Chongqing, P R China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Chongqing Three Gorges Central Hospital, Chongqing, P R China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Deng
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003182:Complement C5 / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D003930:Diabetic Retinopathy / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D003182:Complement C5; D015850:Interleukin-6; D012333:RNA, Messenger