title: Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging.
abstract: Biosynthetic investigation of quinonemethide triterpenoid 22β-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using (13)C-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22β-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes.
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av. Costábile Romano, 2201, 14096-900, Ribeirão Preto, SP, Brazil.
authors.forename: Edieidia S
authors.initials: ES
authors.lastname: Pina
authors.affiliation: Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil.|Universidade Federal de Mato Grosso do Sul (UFMS), Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Campo Grande, MS, 79070-900, Brazil.
authors.forename: Denise B
authors.initials: DB
authors.lastname: Silva
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Laboratório de Botânica, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil.
authors.forename: Simone P
authors.initials: SP
authors.lastname: Teixeira
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av. Costábile Romano, 2201, 14096-900, Ribeirão Preto, SP, Brazil.
authors.forename: Juliana S
authors.initials: JS
authors.lastname: Coppede
authors.affiliation: Instituto de Química, Universidade Estadual Paulista, Araraquara, SP, 14801-970, Brazil.
authors.forename: Maysa
authors.initials: M
authors.lastname: Furlan
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av. Costábile Romano, 2201, 14096-900, Ribeirão Preto, SP, Brazil.
authors.forename: Suzelei C
authors.initials: SC
authors.lastname: França
authors.affiliation: Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil.
authors.forename: Norberto P
authors.initials: NP
authors.lastname: Lopes
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av. Costábile Romano, 2201, 14096-900, Ribeirão Preto, SP, Brazil.
authors.forename: Ana Maria S
authors.initials: AM
authors.lastname: Pereira
authors.affiliation: Unidade de Biotecnologia, Universidade de Ribeirão Preto, Av. Costábile Romano, 2201, 14096-900, Ribeirão Preto, SP, Brazil.
authors.forename: Adriana A
authors.initials: AA
authors.lastname: Lopes
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D066298:In Vitro Techniques; D045563:Indolequinones / Q000737:chemistry; D029562:Magnoliaceae; D053858:Metabolic Networks and Pathways; D008798:Mevalonic Acid / Q000378:metabolism; D053147:Plant Exudates / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013095:Spermidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry
chemical_list: D000975:Antioxidants; D045563:Indolequinones; D053147:Plant Exudates; D014315:Triterpenes; C068040:quinone methide; C008335:maytenine; D008798:Mevalonic Acid; D013095:Spermidine