title: Time dependent impact of perinatal hypoxia on growth hormone, insulin-like growth factor 1 and insulin-like growth factor binding protein-3.
abstract: Hypoxic-ischemia (HI) is a widely used animal model to mimic the preterm or perinatal sublethal hypoxia, including hypoxic-ischemic encephalopathy. It causes diffuse neurodegeneration in the brain and results in mental retardation, hyperactivity, cerebral palsy, epilepsy and neuroendocrine disturbances. Herein, we examined acute and subacute correlations between neuronal degeneration and serum growth factor changes, including growth hormone (GH), insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) after hypoxic-ischemia (HI) in neonatal rats. In the acute phase of hypoxia, brain volume was increased significantly as compared with control animals, which was associated with reduced GH and IGF-1 secretions. Reduced neuronal survival and increased DNA fragmentation were also noticed in these animals. However, in the subacute phase of hypoxia, neuronal survival and brain volume were significantly decreased, accompanied by increased apoptotic cell death in the hippocampus and cortex. Serum GH, IGF-1, and IGFBP-3 levels were significantly reduced in the subacute phase of HI. Significant retardation in the brain and body development were noted in the subacute phase of hypoxia. Here, we provide evidence that serum levels of growth-hormone and factors were decreased in the acute and subacute phase of hypoxia, which was associated with increased DNA fragmentation and decreased neuronal survival.
authors.affiliation: Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Ekinciler cad. 19, TR-34810, Istanbul, Turkey.|Department of Pediatrics, Gulhane Military Medical Academy, Haydarpasa Teaching Hospital, Istanbul, Turkey.
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Kartal
authors.affiliation: Department of Pediatrics, Gulhane Military Medical Academy, Haydarpasa Teaching Hospital, Istanbul, Turkey.
authors.forename: Seçil
authors.initials: S
authors.lastname: Aydınöz
authors.affiliation: Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Ekinciler cad. 19, TR-34810, Istanbul, Turkey.|Department of Pediatrics, Marmaris State Hospital, Mugla, Turkey.
authors.forename: Ayşe Tuğba
authors.initials: AT
authors.lastname: Kartal
authors.affiliation: Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Ekinciler cad. 19, TR-34810, Istanbul, Turkey.
authors.forename: Taha
authors.initials: T
authors.lastname: Kelestemur
authors.affiliation: Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Ekinciler cad. 19, TR-34810, Istanbul, Turkey.
authors.forename: Ahmet Burak
authors.initials: AB
authors.lastname: Caglayan
authors.affiliation: Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Ekinciler cad. 19, TR-34810, Istanbul, Turkey.
authors.forename: Mustafa Caglar
authors.initials: MC
authors.lastname: Beker
authors.affiliation: Department of Pediatrics, Gulhane Military Medical Academy, Haydarpasa Teaching Hospital, Istanbul, Turkey.
authors.forename: Ferhan
authors.initials: F
authors.lastname: Karademir
authors.affiliation: Department of Pediatrics, Gulhane Military Medical Academy, Haydarpasa Teaching Hospital, Istanbul, Turkey.
authors.forename: Selami
authors.initials: S
authors.lastname: Süleymanoğlu
authors.affiliation: Department of Pediatrics, Gulhane Military Medical Academy, Haydarpasa Teaching Hospital, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Kul
authors.affiliation: Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Ekinciler cad. 19, TR-34810, Istanbul, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Yulug
authors.affiliation: Department of Physiology, Regenerative and Restorative Medical Research Center, Istanbul Medipol University, Ekinciler cad. 19, TR-34810, Istanbul, Turkey. ekilic@medipol.edu.tr.
authors.forename: Ertugrul
authors.identifier: 0000-0001-6494-8923
authors.initials: E
authors.lastname: Kilic
mesh_terms: D000818:Animals; D002470:Cell Survival; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D005260:Female; D013006:Growth Hormone / Q000097:blood*; D020925:Hypoxia-Ischemia, Brain / Q000097:blood* / Q000473:pathology; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000473:pathology*; D051381:Rats
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone
keywords: Apoptosis; Brain injury; IGF-1; IGFBP-3 levels; Perinatal hypoxia; Serum GH