title: Biglycan Inhibits Capsaicin-Induced Substance P Release by Cultured Dorsal Root Ganglion Neurons.
abstract: The purpose of this study was to examine the inhibitory effects of biglycan on substance P release from cultured sensory neurons in response to capsaicin.
  
  
  
  In vitro study of cultured primary sensory neurons from the rabbit dorsal root ganglion (DRG). We interrogated the culture system function with capsaicin. Biglycan is an important structural component of the intervertebral disc that may regulate growth factors and inflammatory mediators. We tested the hypothesis that biglycan inhibits substance P release in response to capsaicin.
  
  
  
  The DRG cultures were shown to contain both neurons and astrocytes by immunostaining using antibodies recognizing neuron and glial cell markers. Cultured DRG cells respond to capsaicin in a dose- and time-dependent manner (capsaicin dose ranges from 5 to 500 μmol/L; stimulation time ranges from 0 to 60 minutes). The neurons preincubated with biglycan released 27% less substance P compared with neurons without biglycan (n = 4, P = 0.036).
  
  
  
  We have established a DRG cell culture system, which contains both sensory neurons and the supporting astrocytes. Biglycan, an inhibitor of substance P release by DRG cultures, may serve as an ingredient in intradiscal injectables to reduce back pain.
authors.affiliation: From the Departments of Orthopedic Surgery (PS, AC, SA, HSA), Neurological Sciences (E-YC), and Biochemistry (GC-S), Rush University Medical Center, Chicago, Illinois; Department of Orthopedic Surgery, Boston University Medical Center, Boston, Massachusetts (CT); School of Biomedical Engineering, Science & Health Systems, Drexel University, Philadelphia, Pennsylvania (HL); Departments of Physical Medicine and Rehabilitation (YZ) and Orthopedic Surgery (LQ, YZ), Perelman School of Medicine, University of Pennsylvania; and Translational Musculoskeletal Research Center (TMRC), Philadelphia Veterans Affairs Medical Center (YZ), Philadelphia, Pennsylvania.
authors.forename: Peng
authors.initials: P
authors.lastname: Shi
authors.forename: Er-Yun
authors.initials: EY
authors.lastname: Chen
authors.forename: Gabriella
authors.initials: G
authors.lastname: Cs-Szabo
authors.forename: Ana
authors.initials: A
authors.lastname: Chee
authors.forename: Chadi
authors.initials: C
authors.lastname: Tannoury
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: Han
authors.initials: H
authors.lastname: Lin
authors.forename: Steven
authors.initials: S
authors.lastname: An
authors.forename: Howard S
authors.initials: HS
authors.lastname: An
authors.forename: Yejia
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology; D058578:Biglycan / Q000494:pharmacology*; D002211:Capsaicin / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000166:cytology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D018689:Sensory System Agents / Q000494:pharmacology*; D013373:Substance P / Q000378:metabolism*
chemical_list: D058578:Biglycan; D018689:Sensory System Agents; D013373:Substance P; D002211:Capsaicin