title: Senegenin Ameliorate Acute Lung Injury Through Reduction of Oxidative Stress and Inhibition of Inflammation in Cecal Ligation and Puncture-Induced Sepsis Rats.
abstract: The purpose of this study was to assess the protective effect of senegenin on acute lung injury (ALI) in rats induced by sepsis. Rat ALI model was reproduced by cecal ligation and puncture (CLP). All rats were randomly divided into five groups: group 1 (control), group 2 (CLP), group 3 (CLP + senegenin 15 mg/kg), group 4 (CLP + senegenin 30 mg/kg), and group 5 (CLP + senegenin 60 mg/kg). CLP + senegenin groups received senegenin by gavage daily for consecutive 5 days, respectively, while the mice in control and CLP groups were given an equivalent volume of saline. We detected the lung wet/dry weight ratios and the histopathology of the lung. The levels of lung tissue myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) were determined. Meanwhile, the nuclear factor-kappa B (NF-κB) activation, tumor necrosis factor-alpha (TNF-α), and interleukin-1β (IL-1β) levels were studied. The results demonstrated that senegenin treatment significantly attenuated CLP-induced lung injury, including reduction of lung wet/dry weight ratio, protein leak, infiltration of leukocytes, and MPO activity. In addition, senegenin markedly decreased MDA content and increased SOD activity and GSH level. Serum levels of TNF-α and IL-1β were also decreased by senegenin administration. Furthermore, senegenin administration inhibited the nuclear translocation of NF-κB in the lungs. These findings indicate that senegenin exerts protective effects on CLP-induced septic rats. Senegenin may be a potential therapeutic agent against sepsis.
authors.affiliation: Dispensary of Traditional Chinese Medicine, Yantai Hospital of Traditional Chinese Medicine, Yantai, 264000, China.
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Yantai Hospital of Traditional Chinese Medicine, Yantai, 264000, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Yantai Hospital of Traditional Chinese Medicine, N0.39 Xingfu Road, Zhifu District, Yantai, 264000, China.
authors.forename: Jian-Jie
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Department of Nephrology, Yantai Hospital of Traditional Chinese Medicine, N0.39 Xingfu Road, Zhifu District, Yantai, 264000, China. lihongchun9@hotmail.com.
authors.forename: Shan-Dan
authors.initials: SD
authors.lastname: Feng
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D055371:Acute Lung Injury / Q000188:drug therapy*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D002432:Cecum / Q000601:surgery; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004789:Enzyme Activation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy; D053583:Interleukin-1beta / Q000097:blood; D008168:Lung / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000188:drug therapy / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C000594089:IL1B protein, rat; D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C493220:tenuigenin; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: acute lung injury; inflammation; oxidative stress; senegenin; sepsis