title: The separation and analysis of symmetric and asymmetric dimethylarginine and other hydrophilic isobaric compounds using aqueous normal phase chromatography.
abstract: Two biologically important compounds with clinical relevance, asymmetric dimethylarginine and symmetric dimethylarginine, are analyzed using aqueous normal phase chromatography on silica hydride-based columns. Two different stationary phases were tested, a commercially available Diamond Hydride™ and a 2-acrylamido-2-methylpropane sulfonic acid experimental column. Two types of analytical protocols were investigated: analysis of the compounds when separation was achieved and analysis of the compounds with partial chromatographic separation. Urine samples from tuberculosis patients were tested for levels of asymmetric and symmetric dimethylarginine. The mass spectrometric technique of in-source fragmentation that can provide data similar to a tandem mass analyzer was evaluated as a means of identification and quantitation of the two compounds when complete separation is not achieved. This same protocol was also evaluated for two other isobaric compounds, glucose-1 and glucose-6 phohsphate, and leucine and isoleucine.
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA. Electronic address: joseph.pesek@sjsu.edu.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyksa
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Brent
authors.initials: B
authors.lastname: Modereger
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Hasbun
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Vy T
authors.initials: VT
authors.lastname: Phan
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Mehr
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Mariano
authors.initials: M
authors.lastname: Guzman
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Watanable
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D017640:Silicates; D014397:Tuberculosis, Pulmonary / Q000652:urine; D014867:Water
chemical_list: D017640:Silicates; C487012:silica hydride; D014867:Water; C024917:symmetric dimethylarginine; C018524:N,N-dimethylarginine; D001120:Arginine
keywords: Clinical analyses; In-source fragmentation; Silica hydride columns