title: Lactobacillus acidophilus binds to MUC3 component of cultured intestinal epithelial cells with highest affinity.
abstract: Lactobacillus strains have been shown to adhere to the mucosal components of intestinal epithelial cells. However, established in vitro adhesion assays have several drawbacks in assessing the adhesion of new Lactobacillus strains. The present study aimed to compare the adhesion of four different Lactobacillus strains and select the most adherent microbe, based on in silico approach supported by in vitro results. The mucus-binding proteins in Lactobacillus acidophilus, L. plantarum, L. brevis and L. fermentum were identified and their capacities to interact with intestinal mucin were compared by molecular docking analysis. Lactobacillus acidophilus had the maximal affinity of binding to mucin with predicted free energy of -6.066 kcal mol(-1) Further, in vitro experimental assay of adhesion was performed to validate the in silico results. The adhesion of L. acidophilus to mucous secreting colon epithelial HT-29 MTX cells was highest at 12%, and it formed biofilm with maximum depth (Z = 84 μm). Lactobacillus acidophilus was determined to be the most adherent strain in the study. All the Lactobacillus strains tested in this study, displayed maximum affinity of binding to MUC3 component of mucus as compared to other gastrointestinal mucins. These findings may have importance in the design of probiotics and health care management.
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar-751024, India.
authors.forename: Jugal Kishore
authors.initials: JK
authors.lastname: Das
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar-751024, India.
authors.forename: Rajani Kanta
authors.initials: RK
authors.lastname: Mahapatra
authors.affiliation: Kalinga Institute of Medical Sciences (KIMS), KIIT University, Bhubaneswar-751024, India.
authors.forename: Shubhransu
authors.initials: S
authors.lastname: Patro
authors.affiliation: National Institute of Science Education and Research (NISER), Bhubaneswar-751005, India.
authors.forename: Chandan
authors.initials: C
authors.lastname: Goswami
authors.affiliation: School of Biotechnology, KIIT University, Bhubaneswar-751024, India msbiotek@yahoo.com.
authors.forename: Mrutyunjay
authors.initials: M
authors.lastname: Suar
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism; D005752:Gastric Mucins / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D052196:Levilactobacillus brevis / Q000378:metabolism*; D052197:Limosilactobacillus fermentum / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism*; D062105:Molecular Docking Simulation; D055263:Mucin-3 / Q000378:metabolism*; D019936:Probiotics
chemical_list: D002352:Carrier Proteins; D005752:Gastric Mucins; C524652:MUC3A protein, human; D055263:Mucin-3
keywords: MUC3; adhesion; docking; mucin; mucus-binding proteins