title: Effect of Trolox on sperm quality in normozospermia and oligozospermia during cryopreservation.
abstract: This study evaluated the effects of different concentrations of Trolox supplementation to cryoprotective agent (CPA) on post-thaw apoptosis-like events that include translocation of phosphatidyl serine (PS) to the cell surface, alterations in mitochondrial membrane potential (MMP), and DNA integrity of normozoospermic and oligoozoospermic semen samples. Spermatozoa from 20 normozoospermic men and 20 patients with oligoozoospermia were cryopreserved with cryo-protective agent containing 0, 20, 40, and 80 μM Trolox. Pre-cryopreservation and post-thaw sperm MMP, PS externalization and DNA fragmentation were evaluated by flow cytometry. Sperm frozen in extender with Trolox had greater MMP, lower DNA fragmentation and externalization of PS in both groups, though the most effective dose of Trolox in normozoospermic and oligoozoospermic semen samples were different. These findings support the use of Trolox as freezing extender supplement to improve the quality of cryopreserved human sperm, measured in terms of early apoptosis changes and DNA integrity, in both normozoospermic and oligoozoospermic men.
authors.affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: snekoonam@razi.tums.ac.ir.
authors.forename: Saeid
authors.initials: S
authors.lastname: Nekoonam
authors.affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: m-shabani@razi.tums.ac.ir.
authors.forename: Maryam Shabani
authors.initials: MS
authors.lastname: Nashtaei
authors.affiliation: Urology and Nephrology Research Center (UNRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: naji_m_f@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Naji
authors.affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: minaezb@sina.tums.ac.ir.
authors.forename: Bagher Minaei
authors.initials: BM
authors.lastname: Zangi
authors.affiliation: Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: Famidi@sina.tums.ac.ir.
authors.forename: Fardin
authors.initials: F
authors.lastname: Amidi
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002839:Chromans / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects*; D005615:Freezing; D006801:Humans; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009845:Oligospermia / Q000473:pathology*; D010718:Phosphatidylserines / Q000378:metabolism; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology; D055815:Young Adult
chemical_list: D000975:Antioxidants; D002839:Chromans; D003451:Cryoprotective Agents; D010718:Phosphatidylserines; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
keywords: Cryopreservation; DNA fragmentation; Mitochondrial membrane potential; Oligoozoospermia; Trolox