title: Preparation and Reinforcement of Dual-Porous Biocompatible Cellulose Scaffolds for Tissue Engineering.
abstract: 1Biocompatible cellulose-based aerogels composed of nanoporous struts, which embed interconnected voids of controlled micron-size, have been prepared employing temporary templates of fused porogens, reinforcement by interpenetrating PMMA networks and supercritical carbon dioxide drying. Different combinations of cellulose solvent (Ca(SCN)2/H2O/LiCl or [EMIm][OAc]/DMSO) and anti-solvent (EtOH), porogen type (paraffin wax or PMMA spheres) and porogen size (various fractions in the range of 100-500 μm) as well as intensity of PMMA reinforcement have been investigated to tailor the materials for cell scaffolding applications. All aerogels exhibited an open and dual porosity (micronporosity >100 μm and nanoporosity extending to the low micrometer range). Mechanical properties of the dual-porous aerogels under compressive stress were considerably improved by introduction of interpenetrating PMMA networks. The effect of the reinforcing polymer on attachment, spreading, and proliferation of NIH 3T3 fibroblast cells, cultivated on selected dual-porous aerogels to pre-evaluate their biocompatibility was similarly positive.
authors.affiliation: Department of Chemistry Division of Chemistry of Renewables University of Natural Resources and Life Sciences Vienna Konrad-Lorenz-Straße 24, 3430 Tulln Vienna Austria.
authors.forename: Nicole
authors.initials: N
authors.lastname: Pircher
authors.affiliation: Department of Chemistry Division of Chemistry of Renewables University of Natural Resources and Life Sciences Vienna Konrad-Lorenz-Straße 24, 3430 Tulln Vienna Austria.
authors.forename: David
authors.initials: D
authors.lastname: Fischhuber
authors.affiliation: Institute of Chemistry of Clermont-Ferrand Clermont Université Ecole Nationale Supérieure de Chimie de Clermont-Ferrand BP 1044863000 Clermont-Ferrand France; Institute of Chemistry of Clermont-Ferrand Centre National de la Recherche Scientifique 24 av. des Landais 63171Aubière France.
authors.forename: Leticia
authors.initials: L
authors.lastname: Carbajal
authors.affiliation: Department for Biotechnology University of Natural Resources and Life Sciences Vienna Muthgasse 18, 1190 Wien Vienna Austria.
authors.forename: Christine
authors.initials: C
authors.lastname: Strauß
authors.affiliation: Institute of Chemistry of Clermont-Ferrand Clermont Université Ecole Nationale Supérieure de Chimie de Clermont-Ferrand BP 1044863000 Clermont-Ferrand France; Institute of Chemistry of Clermont-Ferrand Centre National de la Recherche Scientifique 24 av. des Landais 63171Aubière France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Nedelec
authors.affiliation: Department for Biotechnology University of Natural Resources and Life Sciences Vienna Muthgasse 18, 1190 Wien Vienna Austria.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Kasper
authors.affiliation: Department of Chemistry Division of Chemistry of Renewables University of Natural Resources and Life Sciences Vienna Konrad-Lorenz-Straße 24, 3430 Tulln Vienna Austria.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rosenau
authors.affiliation: Department of Chemistry Division of Chemistry of Renewables University of Natural Resources and Life Sciences Vienna Konrad-Lorenz-Straße 24, 3430 Tulln Vienna Austria.
authors.forename: Falk
authors.initials: F
authors.lastname: Liebner
keywords: cellulose aerogels; dual‐porosity; porogens; supercritical anti‐solvent precipitation; tissue engineering