title: Evaluation of mouse embryos produced in vitro after electromagnetic waves exposure; Morphometric study.
abstract: BACKGROUND
  Today, the use of electromagnetic waves in medical diagnostic devices, such as magnetic resonance imaging (MRI), has increased, and many of its biological effects have been reported. The aim of the present study was to assess the biological effects of 1.5 Tesla (T) magnetic resonance imaging (MRI) on fertility and reproductive parameters.
  
  
  METHODS
  Eighty adult male and female NMRI mice (NMRI: Naval Medical Research Institute) of age 6-8 weeks were studied and randomly divided into two study and control groups. After confirmation of pregnancy, the mice in the study group were exposed to the MRI (1.5 T) machine's waves over the next three weeks, once a week for 36 minutes. One day and thirty-five days after the last radiation, the mice were killed in order to do the in vitro fertilization (IVF) by neck beads' displacement and the impact on the evolution of embryos, and its quality was studied. Data were analyzed using SPSS version 20 and the significance level of less than 0.05 was considered.
  
  
  RESULTS
  Embryo morphometry showed that the total diameter and the cytoplasm diameter of the study group embryos suffered significant reduction compared to the control group, 1 day after the last irradiation (p < 0.05), but the diameter of the perivitelline space of this group's embryos had a significant increase (p < 0.05). The qualitative results during 35 days after irradiation showed that morphologically parameters of the embryos in the study group had no significant differences from the control group.
  
  
  CONCLUSIONS
  Exposure to MRI irradiation can transiently disturb the development of mouse embryos and fertility, but these effects are reversible 35 days after the last irradiation.
authors.affiliation: M.Sc. of Anatomy, Faculty Member, Department of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: Ayoob
authors.initials: A
authors.lastname: Rostamzadeh
authors.affiliation: Ph.D. of Pharmaceutical Biotechnology, Assistant Professor, Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mohammadi
authors.affiliation: Ph.D. Candidate of Clinical Biochemistry, Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ahmadi
authors.affiliation: Ph.D. of Physiology, Assistant Professor, Department of Physiology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Nazari
authors.affiliation: Ph.D. Candidate of Pharmaceutical Biotechnology, Department of Pharmaceutical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Omar
authors.initials: O
authors.lastname: Ghaderi
authors.affiliation: Ph.D. of Anatomy, Assistant Professor, Department of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Anjomshoa
keywords: MRI (Magnetic resonance imaging); embryo morphometry; fertility