title: The preclinical study of predicting radiosensitivity in human nasopharyngeal carcinoma xenografts by 18F-ML-10 animal- PET/CT imaging.
abstract: Previous studies have reported that the radiosensitivity is associated with apoptosis. Hereby, we aimed to investigate the value of 18F-ML-10 PET/CT, which selectively targeted cells undergoing apoptosis, in predicting radiosensitivity of human nasopharyngeal carcinoma (NPC) xenografts. We used CNE1 (highly differentiated) and CNE2 (poorly differentiated) NPC cell lines to construct tumor models, which had very different radiosensitivities. After irradiation, the volumes of CNE2 tumors decreased significantly while those of CNE1 tumors increased. In 18F-ML-10 imaging, the values of tumor/muscle (T/M) between CNE1 and CNE2 mice were statistically different at both 24 h and 48 h after irradiation. Besides, ΔT/M1-0 and ΔT/M2-0 of CNE2 mice were higher than those of CNE1 mice, demonstrating obvious discrepancy. Furthermore, we observed obvious changes of radioactive distribution in CNE2 group. On the contrary, T/M of 18F-FDG in irradiation group revealed no obvious change in both CNE1 and CNE2 groups. In conclusion, 18F-ML-10 animal PET/CT showed its potential to predict radiosensitivity in NPC.
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Bao
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Siyang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Gu
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Shanghai 200032, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.|Center for Biomedical Imaging, Fudan University, Shanghai 200032, China.|Shanghai Engineering Research Center for Molecular Imaging Probes, Shanghai 200032, China.
authors.forename: Yingjian
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002277:Carcinoma / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D008764:Methylmalonic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy; D000072078:Positron Emission Tomography Computed Tomography / Q000379:methods*; D011836:Radiation Tolerance; D011877:Radionuclide Imaging / Q000379:methods*; D019275:Radiopharmaceuticals / Q000378:metabolism; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: C532430:5-fluoropentyl-2-methylmalonic acid; D019275:Radiopharmaceuticals; D008764:Methylmalonic Acid
keywords: 18F-ML-10; animal-PET/CT; irradiation sensitivity; nasopharyngeal carcinoma; nude mouse