title: Effects of Trigonelline, an Alkaloid Present in Coffee, on Diabetes-Induced Disorders in the Rat Skeletal System.
abstract: Diabetes increases bone fracture risk. Trigonelline, an alkaloid with potential antidiabetic activity, is present in considerable amounts in coffee. The aim of the study was to investigate the effects of trigonelline on experimental diabetes-induced disorders in the rat skeletal system. Effects of trigonelline (50 mg/kg p.o. daily for four weeks) were investigated in three-month-old female Wistar rats, which, two weeks before the start of trigonelline administration, received streptozotocin (60 mg/kg i.p.) or streptozotocin after nicotinamide (230 mg/kg i.p.). Serum bone turnover markers, bone mineralization, and mechanical properties were studied. Streptozotocin induced diabetes, with significant worsening of bone mineralization and bone mechanical properties. Streptozotocin after nicotinamide induced slight glycemia increases in first days of experiment only, however worsening of cancellous bone mechanical properties and decreased vertebral bone mineral density (BMD) were demonstrated. Trigonelline decreased bone mineralization and tended to worsen bone mechanical properties in streptozotocin-induced diabetic rats. In nicotinamide/streptozotocin-treated rats, trigonelline significantly increased BMD and tended to improve cancellous bone strength. Trigonelline differentially affected the skeletal system of rats with streptozotocin-induced metabolic disorders, intensifying the osteoporotic changes in streptozotocin-treated rats and favorably affecting bones in the non-hyperglycemic (nicotinamide/streptozotocin-treated) rats. The results indicate that, in certain conditions, trigonelline may damage bone.
authors.affiliation: Department of Pharmacology, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, 40-055 Katowice, Poland. jfolwarczna@sum.edu.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Folwarczna
authors.affiliation: Department of Pharmacology, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, 40-055 Katowice, Poland. ajanas@sum.edu.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Janas
authors.affiliation: Department of Pharmacology, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, 40-055 Katowice, Poland. mariapytlik@gmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Pytlik
authors.affiliation: Department of Pharmacology, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, 40-055 Katowice, Poland. ucegiela@o2.pl.
authors.forename: Urszula
authors.initials: U
authors.lastname: Cegieła
authors.affiliation: Department of Pharmacology, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, 40-055 Katowice, Poland. lsliw@o2.pl.
authors.forename: Leszek
authors.initials: L
authors.lastname: Śliwiński
authors.affiliation: Department of Clinical and Experimental Pharmacotherapy, Medical Faculty, Slovak Medical University, 833 03 Bratislava, Slovakia. zora.krivosikova@szu.sk.
authors.forename: Zora
authors.initials: Z
authors.lastname: Krivošíková
authors.affiliation: Department of Clinical and Experimental Pharmacotherapy, Medical Faculty, Slovak Medical University, 833 03 Bratislava, Slovakia. kornelia.stefikova@szu.sk.
authors.forename: Kornélia
authors.initials: K
authors.lastname: Štefíková
authors.affiliation: Department of Clinical and Experimental Pharmacotherapy, Medical Faculty, Slovak Medical University, 833 03 Bratislava, Slovakia. martin.gajdos@szu.sk.
authors.forename: Martin
authors.initials: M
authors.lastname: Gajdoš
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D015519:Bone Density / Q000187:drug effects; D040503:Coffea / Q000737:chemistry*; D048909:Diabetes Complications / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D005269:Femur / Q000187:drug effects / Q000503:physiopathology; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000633:toxicity*; D008159:Lumbar Vertebrae / Q000187:drug effects / Q000503:physiopathology; D009536:Niacinamide; D010024:Osteoporosis / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D010946:Plants, Medicinal; D017208:Rats, Wistar; D018570:Risk Assessment; D012639:Seeds; D013311:Streptozocin; D013977:Tibia / Q000187:drug effects / Q000503:physiopathology; D016474:Weight-Bearing
chemical_list: D000470:Alkaloids; D015415:Biomarkers; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D009536:Niacinamide; C009560:trigonelline; D013311:Streptozocin
keywords: coffee; diabetes; rats; skeletal system; streptozotocin; trigonelline