title: [Characterization of the Lipopolysaccharides of Pseudomonas chlororaphis].
abstract: Lipopolysaccharides (LPS) from two strains ot Pseudomonas chlororaphis subsp. aureofaciens,UCM B-111 and UCM B-306, were isolated and characterized. The LPS preparations exhibited low toxicity, high pyrogenicity and high antiviral activity. Mild acid hydrolysis was used to obtain the O-specific polysaccharides. Their structures were established by monosaccharide analysis and determination of absolute configurations, as well as by 1D and 2D NMR spectroscopy. The O-polysaccharides were shown to contain the linear tri- or tetrasaccharide repeating units. Both O-polysaccharides were structurally heterogeneous: P. chlororaphis subsp. aureofaciens UCM B-111--> 4)-αD-GalpNAc6Ac-(1 --> 3)-β-D-QuipNAc-(1 --> 6)-αD-GlcpNAc-(l --> βD-GlcpNAc-(l --> 3)] GalNAc -60%; degree of the non-stoichiometric 6-O-acetylation of GalNAc -60%; P. chlorophis subsp. aureofaciens UCM B-306 --> 3)-α-D-Rhap-(1 --> 4)-α-D-GalpNAcAN-(1 --> 3)-αD-QuipNAc4NAc-(1 -->, where GalNAcAN is 2-acetamido-2-deoxy-D-galacturonamide, the degree of non-stoichiometric amidation of the GalNAcA residue -60%.
authors.forename: L D
authors.initials: LD
authors.lastname: Varbanets
authors.forename: E L
authors.initials: EL
authors.lastname: Zdorovenko
authors.forename: E A
authors.initials: EA
authors.lastname: Kiprianova
authors.forename: L V
authors.initials: LV
authors.lastname: Avdeeva
authors.forename: O S
authors.initials: OS
authors.lastname: Brovarskaya
authors.forename: S L
authors.initials: SL
authors.lastname: Rybalko
mesh_terms: D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000378:metabolism; D011549:Pseudomonas / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides