title: Relationship between the Peroxidation of Leukocytes Index Ratio and the Improvement of Postprandial Metabolic Stress by a Functional Food.
abstract: For the first time, we investigated the relationship between postprandial dysmetabolism and the Peroxidation of Leukocytes Index Ratio (PLIR), a test that measures the resistance of leukocytes to exogenous oxidative stress and their functional capacity of oxidative burst upon activation. Following a blind, placebo controlled, randomized, crossover design, ten healthy subjects ingested, in two different occasions, a high fat and high carbohydrates meal with Snello cookie (HFHCM-S) or with control cookies (HFHCM-C). Snello cookie, a functional food covered by dark chocolate and containing glucomannan, inulin, fructooligosaccharides, and Bacillus coagulans strain GanedenBC30, significantly improved postprandial metabolic stress (insulin, glucose, and triglycerides) and reduced the postprandial increase of uric acid. HFHCM-S improved PLIR of lymphocytes, but not of monocytes and granulocytes. Both meals increased granulocytes' count and reduced the lipoperoxidation induced by both exogenous free radicals and reactive oxygen species (ROS) produced by oxidative burst. Our results suggest that the healthy status of the subjects could be a limitation of this pilot study for PLIR evaluation on cells that produce ROS by oxidative burst. In conclusion, the relationship between PLIR and postprandial dysmetabolism requires further investigations.
authors.affiliation: Center of Nutrition, Council for Agricultural Research and Economics (CREA-NUT), Via Ardeatina 546, 00178 Rome, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Peluso
authors.affiliation: Department of Physiology and Pharmacology "V. Erspamer", "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Husseen
authors.initials: H
authors.lastname: Manafikhi
authors.affiliation: Department of Physiology and Pharmacology "V. Erspamer", "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Reggi
authors.affiliation: Department of Physiology and Pharmacology "V. Erspamer", "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Yaroslava
authors.initials: Y
authors.lastname: Longhitano
authors.affiliation: Department of Physiology and Pharmacology "V. Erspamer", "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Christian
authors.initials: C
authors.lastname: Zanza
authors.affiliation: Department of Physiology and Pharmacology "V. Erspamer", "Sapienza" University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Palmery
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D019518:Postprandial Period; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D017382:Reactive Oxygen Species