title: Improved muscle function and quality after diet intervention with leucine-enriched whey and antioxidants in antioxidant deficient aged mice.
abstract: Antioxidant (AOX) deficiencies are commonly observed in older adults and oxidative stress has been suggested to contribute to sarcopenia. Here we investigate if 1) low levels of dietary antioxidants had a negative impact on parameters of muscle mass, function and quality, and 2) to study if nutritional interventions with AOX and/or leucine-enriched whey protein could improve these muscle parameters in aged mice. 18-months-old mice were fed a casein-based antioxidant-deficient (lowox) diet or a casein-based control-diet (CTRL) for 7 months. During the last 3 months, lowox-mice were subjected to either: a) continued lowox, b) supplementation with vitamin A/E, Selenium and Zinc (AOX), c) substitution of casein with leucine-enriched whey protein (PROT) or d) a combination of both AOX and PROT (TOTAL). After 7 months lowox-mice displayed lower muscle strength and more muscle fatigue compared to CTRL. Compared to lowox-mice, PROT-mice showed improved muscle power, grip strength and less muscle fatigue. AOX-mice showed improved oxidative status, less muscle fatigue, improved grip strength and mitochondrial dynamics compared to lowox-mice. The TOTAL-mice showed the combined effects of both interventions compared to lowox-mice. In conclusion, nutritional intervention with AOX and/or leucine-enriched whey protein can play a role in improving muscle health in a AOX-deficient mouse model.
authors.affiliation: Nutricia Research, Nutricia Advanced Medical Nutrition, Utrecht, The Netherlands.
authors.forename: Miriam
authors.initials: M
authors.lastname: van Dijk
authors.affiliation: Nutricia Research, Nutricia Advanced Medical Nutrition, Utrecht, The Netherlands.
authors.forename: Francina J
authors.initials: FJ
authors.lastname: Dijk
authors.affiliation: Department of Animal Sciences, Human and Animal Physiology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Annelies
authors.initials: A
authors.lastname: Bunschoten
authors.affiliation: Department of Animal Sciences, Human and Animal Physiology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Dorien A M
authors.initials: DA
authors.lastname: van Dartel
authors.affiliation: Nutrition and Pharmacology, Wageningen University, Wageningen, The Netherlands.
authors.forename: Klaske
authors.initials: K
authors.lastname: van Norren
authors.affiliation: Unite de Nutrition Humaine, INRA-UdA, Clermont-Ferrand, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Walrand
authors.affiliation: Nutricia Research, Nutricia Advanced Medical Nutrition, Utrecht, The Netherlands.
authors.forename: Marion
authors.initials: M
authors.lastname: Jourdan
authors.affiliation: Nutricia Research, Nutricia Advanced Medical Nutrition, Utrecht, The Netherlands.
authors.forename: Sjors
authors.initials: S
authors.lastname: Verlaan
authors.affiliation: Nutricia Research, Nutricia Advanced Medical Nutrition, Utrecht, The Netherlands.
authors.forename: Yvette
authors.initials: Y
authors.lastname: Luiking
mesh_terms: D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D004032:Diet; D007930:Leucine / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology; D011897:Random Allocation; D000067816:Whey Proteins / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D000067816:Whey Proteins; D007930:Leucine
keywords: Gerotarget; antioxidants; fatigue; sarcopenia; skeletal muscle