title: Prevalence of Porphyromonas gingivalis fimA genotypes in the peri-implant sulcus of Koreans assessed using a new primer.
abstract: OBJECTIVE
  Porphyromonas gingivalis fimA is a virulence factor associated with periodontal diseases, but its role in the pathogenesis of peri-implantitis remains unclear. We aimed to evaluate the relationship between the condition of peri-implant tissue and the distribution of P. gingivalis fimA genotypes in Koreans using a new primer.
  
  
  METHODS
  A total of 248 plaque samples were taken from the peri-implant sulci of 184 subjects. The control group consisted of sound implants with a peri-implant probing depth (PD) of 5 mm or less with no bleeding on probing (BOP). Test group I consisted of implants with a peri-implant PD of 5 mm or less and BOP, and test group II consisted of implants with a peri-implant PD of more than 5 mm and BOP. DNA was extracted from each sample and analyzed a using a polymerase chain reaction (PCR) with P. gingivalis-specific primers, followed by an additional PCR assay to differentiate the fimA genotypes in P. gingivalis-positive subjects.
  
  
  RESULTS
  The Prevalence of P. gingivalis in each group did not significantly differ (P>0.05). The most predominant fimA genotype in all groups was type II. The prevalence of type Ib fimA was significantly greater in test group II than in the control group (P<0.05).
  
  
  CONCLUSIONS
  The fimA type Ib genotype of P. gingivalis was found to play a critical role in the destruction of peri-implant tissue, suggesting that it may be a distinct risk factor for peri-implantitis.
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea .
authors.forename: Sung-Geun
authors.identifier: 0000-0002-4698-5306
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.; Department of Periodontology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Ji-Youn
authors.identifier: 0000-0003-1040-7077
authors.initials: JY
authors.lastname: Hong
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.; Department of Periodontology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Seung-Il
authors.identifier: 0000-0001-8762-6169
authors.initials: SI
authors.lastname: Shin
authors.affiliation: Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Korea .
authors.forename: Ji-Hoi
authors.identifier: 0000-0003-0286-5297
authors.initials: JH
authors.lastname: Moon
authors.affiliation: Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Korea .
authors.forename: Jin-Yong
authors.identifier: 0000-0002-4193-0445
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.; Department of Periodontology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea.
authors.forename: Yeek
authors.identifier: 0000-0001-9243-7119
authors.initials: Y
authors.lastname: Herr
keywords: Fimbriae; Peri-implantitis; Periodontal diseases; Virulence factors