title: A single marker choice strategy in simultaneous characterization and quantification of multiple components by rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (RRLC-QqQ-MS).
abstract: Single standard to determine multi-components (SSDMC) method has been accepted as an efficient technique for the quality control of Traditional Chinese medicines (TCMs), especially for overcoming the shortage of reference standards. HPLC-UV methods have been applied to establish SSDMC method for quantitative analysis in several plant medicines and Chinese patent medicines, however, no LC-MS methods have been used. The purpose of this study is to put forward an improved strategy for the choice of single marker in SSDMC using rapid resolution liquid chromatography coupled with triple quadrupole tandem mass spectrometry (RRLC-QqQ-MS). Five different Panax genus plants, recorded in the Chinese Pharmacopeia 2015 edition, were used as research subjects. An improved SSDMC strategy for simultaneous characterization and determination of 18 bioactive saponins in five Panax plants was put forward, and which was validated to be more superior. Then, it was fully investigated with respect to linearity, LODs, LOQs, precision and accuracy. Coupling with multivariate statistical analysis, the established and validated SSDMC strategy could be successively used in discrimination of the five Panax genus plants.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Zhangchi
authors.initials: Z
authors.lastname: Ning
authors.affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Song
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Yunzhuo
authors.initials: Y
authors.lastname: Dong
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Zeng
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yisong
authors.initials: Y
authors.lastname: Shu
authors.affiliation: Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China. Electronic address: lv_cheng0816@163.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. Electronic address: yyliu_1980@163.com.
authors.forename: Yuanyan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. Electronic address: aipinglu@hkbu.edu.hk.
authors.forename: Aiping
authors.initials: A
authors.lastname: Lu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Panax plants; Partial least squares discriminant analysis (PLS-DA); Principal component analysis (PCA); Quantitative analysis of multi-components with single marker (SSDMC); Rapid resolution liquid chromatography coupled with triple quadrupoletandem mass spectrometry (RRLC-QqQ-MS)