title: Detection of a single enzyme molecule based on a solid-state nanopore sensor.
abstract: The nanopore sensor as a high-throughput and low-cost technology can detect a single molecule in a solution. In the present study, relatively large silicon nitride (Si3N4) nanopores with diameters of ∼28 and ∼88 nm were fabricated successfully using a focused Ga ion beam. We have used solid-state nanopores with various sizes to detect the single horseradish peroxidase (HRP) molecule and for the first time analyzed single HRP molecular translocation events. In addition, a real-time monitored single enzyme molecular biochemical reaction and a translocation of the product of enzyme catalysis substrates were investigated by using a Si3N4 nanopore. Our nanopore system showed a high sensitivity in detecting single enzyme molecules and a real-time monitored single enzyme molecular biochemical reaction. This method could also be significant for studying gene expression or enzyme dynamics at the single-molecule level.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, No. 2, Sipailou, Nanjing 210096, People's Republic of China.
authors.forename: ShengWei
authors.initials: S
authors.lastname: Tan
authors.forename: DeJian
authors.initials: D
authors.lastname: Gu
authors.forename: Hang
authors.initials: H
authors.lastname: Liu
authors.forename: QuanJun
authors.initials: Q
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006735:Horseradish Peroxidase / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D008958:Models, Molecular; D058608:Nanopores; D017655:Silicon Compounds / Q000737:chemistry
chemical_list: D017655:Silicon Compounds; D006735:Horseradish Peroxidase; C032734:silicon nitride