title: Stimuli-responsive poly(N-isopropyl acrylamide)-co-tyrosine@gadolinium: Iron oxide nanoparticle-based nanotheranostic for cancer diagnosis and treatment.
abstract: In this paper, we have prepared a stimuli-responsive polymer modified gadolinium doped iron oxide nanoparticle (poly@Gd-MNPs) as cancer theranostic agent. The responsive polymer is composed of the poly(N-isopropyl acrylamide)-co-tyrosine unit, which shows excellent loading for the anti-cancer drug (methotrexate) and stimuli dependent release (change in pH and temperature). The in vitro experiment revealed that the poly@Gd-MNPs exhibited T1-weighted MRI capability (r1=11.314mM(-1)s(-1)) with good in-vitro hyperthermia response. The prepared poly@Gd-MNPs has generated quick heating (45°C in 2min) upon exposure to an alternating magnetic field and able to travel a distance of 35cm in 1min in the presence of an external magnet. The poly@Gd-MNPs shows 86% of drug loading capacity with 70% drug release in first 2h. The cytotoxic assay (MTT) demonstrated that the nanoparticle did not affect the viability of normal human fibroblast and efficiently kill the MCF7 cancer cells in the presence of an external magnetic field. To explore the uptake of poly@Gd-MNPs in the cells, bright field cell imaging study was also performed. This study provides a valuable approach for the design of highly sensitive polymer modified gadolinium doped iron oxide-based T1 contrast agents for cancer theranostics.
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Ekta
authors.initials: E
authors.lastname: Roy
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Patra
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad, Jharkhand 826 004, India. Electronic address: rshmmadhuri@gmail.com.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Madhuri
authors.affiliation: Functional Nanomaterials Research Laboratory, Department of Applied Physics, Indian School of Mines, Dhanbad, Jharkhand 826 004, India.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Sharma
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000964:Antimetabolites, Antineoplastic / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport; D002460:Cell Line; D003287:Contrast Media / Q000737:chemistry*; D004337:Drug Carriers; D004339:Drug Compounding; D065546:Drug Liberation; D005290:Ferric Compounds / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005682:Gadolinium / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006979:Hyperthermia, Induced; D061986:MCF-7 Cells; D060526:Magnetic Fields; D059346:Magnets; D008727:Methotrexate / Q000378:metabolism / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009928:Organ Specificity; D013696:Temperature; D000068936:Theranostic Nanomedicine / Q000379:methods*; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D000964:Antimetabolites, Antineoplastic; D003287:Contrast Media; D004337:Drug Carriers; D005290:Ferric Compounds; C000499:ferric oxide; C052970:poly-N-isopropylacrylamide; D014443:Tyrosine; D005682:Gadolinium; D008727:Methotrexate
keywords: Dual responsive polymer; Gadolinium doped iron oxide nanoparticle; Hyperthermia; T(1) contrast agent; Targeted drug delivery