title: Characterization and occurrence of squash chlorotic leaf spot virus, a tentative new torradovirus infecting cucurbits in Sudan.
abstract: During a survey conducted in Sudan in 2012, a virus with spherical particles was isolated from a squash plant showing chlorotic leaf spots. The virus was transmitted mechanically and by two whitefly species, but not by aphids. RT-PCR with generic torradovirus primers yielded a band of expected size from total RNA of a symptomatic plant. Next-generation sequencing confirmed that this is tentatively a new torradovirus, for which we propose the name 'squash chlorotic leaf spot virus'. Using specific RT-PCR primers, the virus was detected in cucurbit samples collected since 1992 at different locations in Sudan.
authors.affiliation: INRA, UR0407 Pathologie Végétale, 84143, Montfavet, France.
authors.forename: H
authors.initials: H
authors.lastname: Lecoq
authors.affiliation: INRA, UR0407 Pathologie Végétale, 84143, Montfavet, France.
authors.forename: E
authors.initials: E
authors.lastname: Verdin
authors.affiliation: INRA, UR0407 Pathologie Végétale, 84143, Montfavet, France.|INRA, UMR1318, Institut Jean-Pierre Bourgin, 78026, Versailles cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Tepfer
authors.affiliation: INRA, UR0407 Pathologie Végétale, 84143, Montfavet, France.
authors.forename: C
authors.initials: C
authors.lastname: Wipf-Scheibel
authors.affiliation: INRA, UR0407 Pathologie Végétale, 84143, Montfavet, France.
authors.forename: P
authors.initials: P
authors.lastname: Millot
authors.affiliation: Plant Pathology Center, University of Gezira, Wad Medani, Sudan.
authors.forename: G
authors.initials: G
authors.lastname: Dafalla
authors.affiliation: INRA, UR0407 Pathologie Végétale, 84143, Montfavet, France. cecile.desbiez@avignon.inra.fr.
authors.forename: C
authors.identifier: http://orcid.org/0000-0001-8691-7423
authors.initials: C
authors.lastname: Desbiez
mesh_terms: D000818:Animals; D028464:Cucurbita / Q000821:virology*; D004199:Disease Vectors; D006430:Hemiptera / Q000821:virology; D010802:Phylogeny; D010849:Picornaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D018749:RNA, Plant / Q000235:genetics; D013397:Sudan
chemical_list: D018749:RNA, Plant
keywords: Melon; Next-generation sequencing; SCLSV; Squash; Whitefly