title: Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix.
abstract: Dual thermo- and pH-responsive chromatography has been proposed using poly(N-isopropylacrylamide-co-butyl methacrylate-co-N,N-dimethylaminopropyl acrylamide) (P(NIPAM-co-BMA-co-DMAPAAM)) brushes grafted gigaporous polystyrene microspheres (GPM) as matrix. Atom transfer radical polymerization (ATRP) initiator was first coupled onto GPM through Friedel-Crafts acylation with 2-bromoisobutyryl bromide. The dual-responsive polymer brushes were then grafted onto GPM via surface-initiated ATRP. The surface composition, gigaporous structure, protein adsorption and dual-responsive chromatographic properties of the matrix (GPM-P(NIPAM-co-BMA-co-DMAPAAM) were characterized in detail. Results showed that GPM were successfully grafted with thermoresponsive cationic polymer brushes and that the gigaporous structure was well maintained. A column packed with GPM-P(NIPAM-co-BMA-co-DMAPAAM presented low backpressure, good permeability and appreciable thermo-responsibility. By changing pH of the mobile phase and temperature of the column in turn, the column can separate three model proteins at the mobile phase velocity up to 2528cmh(-1). A separation mechanism of this matrix was also proposed. All results indicate that the dual thermo- and pH-responsive chromatography matrix has great potentials in 'green' high-speed protein chromatography.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China. Electronic address: jbqu@upc.edu.cn.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
authors.forename: Jun-Yi
authors.initials: JY
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
authors.forename: Jing-Bin
authors.initials: JB
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Heavy Oil Processing, Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, PR China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Liu
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry*; D008863:Microspheres; D009211:Myoglobin / Q000032:analysis; D058105:Polymerization; D011137:Polystyrenes / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis; D013696:Temperature; D014357:Trypsin / Q000032:analysis
chemical_list: D000178:Acrylamides; D008689:Methacrylates; D009211:Myoglobin; D011137:Polystyrenes; D011506:Proteins; D012709:Serum Albumin; C513218:poly(N-isopropylacrylamide-co-butyl methacrylate-co-N,N-dimethylaminopropylacrylamide); D014357:Trypsin
keywords: Atom transfer radical polymerization; Gigaporous polystyrene microspheres; High-speed protein chromatography; Protein separation; Thermo- and pH-responsive polymer brushes