title: Antioxidant activity of Inonotus obliquus polysaccharide and its amelioration for chronic pancreatitis in mice.
abstract: Inonotus obliquus polysaccharide (IOP) was extracted by water with a yield of 9.83% and purified by an anion-exchange DEAE cellulose column and Sephadex G-200 gel with a polysaccharide content of 98.6%. The scavenging activities for 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and hydroxyl radicals of IOP were 82.3% and 81.3% respectively at a concentration of 5 mg/mL. IOP was composed of Man, Rha, Glu, Gal, Xyl and Ara in a molar ratio of 9.81:3.6：29.1：20.5：21.6：5.4 respectively. The gel permeation chromatography indicated that IOP was a homogeneous polysaccharide with molecular weight of 32.5 kDa. IOP helped to alleviate pancreatic acinar atrophy and weight loss for chronic pancreatitis (CP) mice induced by Diethyldithiocarbamate (DDC). The SOD level was increased most by IOP-H treatment (400 mg/kg body weight). MDA, IL-1β and LDH were significantly decreased by IOP treatment, especially hydroxyproline, IFN-γ and AMS levels were decreased 39.18%, 37.82% and 41.57% by IOP-H treatment respectively compared to MC group. In conclusion, IOP possessed strong antioxidant activity for scavenging free radicals in vitro and vivo which could be propitious to CP therapy in mice.
authors.affiliation: College of Resources and Environmental science, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Resources and Environmental science, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Koukou
authors.initials: K
authors.lastname: Li
authors.affiliation: College of Resources and Environmental science, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Ren
authors.affiliation: College of Resources and Environmental science, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Resources and Environmental science, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: juanjuan4050234@163.com.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Qu
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001487:Basidiomycota / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D006909:Hydroxyproline / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000097:blood; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D050500:Pancreatitis, Chronic / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D016207:Cytokines; D062610:Fungal Polysaccharides; D009005:Monosaccharides; D007770:L-Lactate Dehydrogenase; D000681:Amylases; D006909:Hydroxyproline
keywords: Anti-inflammation; Antioxidant activity; Chronic pancreatitis; Inonotus obliquus polysaccharide