title: 3D Raman imaging of systemic endothelial dysfunction in the murine model of metastatic breast cancer.
abstract: It was recently reported in the murine model of metastatic breast cancer (4T1) that tumor progression and development of metastasis is associated with systemic endothelial dysfunction characterized by impaired nitric oxide (NO) production. Using Raman 3D confocal imaging with the analysis of the individual layers of the vascular wall combined with AFM endothelial surface imaging, we demonstrated that metastasis-induced systemic endothelial dysfunction resulted in distinct chemical changes in the endothelium of the aorta. These changes, manifested as a significant increase in the protein content (18%) and a slight decrease in the lipid content (4%), were limited to the endothelium and did not occur in the deeper layers of the vascular wall. The altered lipid to protein ratio in the endothelium, although more pronounced in the fixed vascular wall, was also observed in the freshly isolated unfixed vascular wall samples in the aqueous environment (12 and 7% change of protein and lipid content, respectively). Our results support the finding that the metastasis induces systemic endothelial dysfunction that may contribute to cancer progression.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.|Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
authors.forename: Marta Z
authors.initials: MZ
authors.lastname: Pacia
authors.affiliation: Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Buczek
authors.affiliation: Department of Experimental Oncology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114, Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Blazejczyk
authors.affiliation: Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Gregorius
authors.affiliation: Department of Experimental Oncology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114, Wroclaw, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wietrzyk
authors.affiliation: Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.|Department of Experimental Pharmacology, Jagiellonian University, Grzegorzecka 16, 31-531, Krakow, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.|Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland. kaczor@chemia.uj.edu.pl.|Jagiellonian Centre of Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348, Krakow, Poland. kaczor@chemia.uj.edu.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kaczor
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000503:physiopathology; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000000981:diagnostic imaging* / Q000503:physiopathology; D005260:Female; D051379:Mice; D018625:Microscopy, Atomic Force; D009362:Neoplasm Metastasis; D013059:Spectrum Analysis, Raman / Q000379:methods*
keywords: 3D Raman imaging; Cancer metastasis; Endothelium; Ex vivo; Pathology marker; Protein overproduction