title: Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report.
abstract: Within the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses - [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience - [2]; Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: the SPIDIA experience - [3]. DNA quality parameters were used to evaluate the laboratory performance within an External Quality Assessment (EQA) [Second SPIDIA-DNA External Quality Assessment (EQA): Influence of pre-analytical phase of blood samples on genomic DNA quality - [4]. These parameters included DNA purity and yield by UV spectrophotometric measurements, the presence of PCR interferences by Kineret software and genomic DNA integrity analysis by Pulsed Field Gel Electrophoresis. Here we present the specific laboratory report of the 2nd SPIDIA-DNA EQA as an example of data and performances evaluation.
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Malentacchi
authors.affiliation: Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Pizzamiglio
authors.affiliation: QIAGEN GmbH, Hilden, Germany.
authors.forename: Hady
authors.initials: H
authors.lastname: Ibrahim-Gawel
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Pazzagli
authors.affiliation: Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Verderio
authors.affiliation: Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
authors.forename: Chiara Maura
authors.initials: CM
authors.lastname: Ciniselli
authors.affiliation: QIAGEN GmbH, Hilden, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Wyrich
authors.affiliation: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Gelmini