title: The hypophagic response to heat stress is not mediated by GPR109A or peripheral β-OH butyrate.
abstract: Rising temperatures resulting from climate change will increase the incidence of heat stress, negatively impacting the labor force and food animal production. Heat stress elevates circulating β-OH butyrate, which induces vasodilation through GPR109a. Interestingly, both heat stress and intraperitoneal β-OH butyrate administration induce hypophagia. Thus, we aimed to investigate the role of β-OH butyrate in heat stress hypophagia in mice. We found that niacin, a β-OH butyrate mimetic that cannot be oxidized to generate ATP, also reduces food intake. Interestingly, the depression in food intake as a result of 8-h intraperitoneal niacin or 48-h heat exposure did not result from changes in hypothalamic expression of orexigenic or anorexigenic signals (AgRP, NPY, or POMC). Genetically eliminating GPR109a expression did not prevent the hypophagic response to heat exposure, intraperitoneal β-OH butyrate (5.7 mmol/kg), or niacin (0.8 mmol/kg). Hepatic vagotomy eliminated the hypophagic response to β-OH butyrate and niacin but did not affect the hypophagic response to heat exposure. We subsequently hypothesized that the hypophagic response to heat stress may depend on direct effects of β-OH butyrate at the central nervous system: β-OH butyrate induced hormonal changes (hyperinsulinemia, hypercorticosteronemia, and hyperleptinemia), or gene expression changes. To test these possibilities, we blocked expression of hepatic hydroxyl methyl glutaryl CoA synthase II (HMGCS2) to prevent hepatic β-OH butyrate synthesis. Mice that lack HMGCS2 maintain a hypophagic response to heat stress. Herein, we establish that the hypophagia of heat stress is independent of GPR109a, the hepatic vagus afferent nerve, and hepatic ketone body synthesis.
authors.affiliation: School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, Arizona.
authors.forename: Chelsea
authors.initials: C
authors.lastname: Hepler
authors.affiliation: School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, Arizona.
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Foy
authors.affiliation: School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, Arizona.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Higgins
authors.affiliation: School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, Arizona bjrenquist@email.arizona.edu.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Renquist
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000494:pharmacology*; D000818:Animals; D004435:Eating; D018882:Heat Stress Disorders / Q000473:pathology*; D006358:Hot Temperature / Q000009:adverse effects*; D007657:Ketone Bodies / Q000096:biosynthesis; D008099:Liver / Q000294:innervation / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009525:Niacin / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism*; D014630:Vagus Nerve / Q000502:physiology; D014803:Vitamin B Complex / Q000494:pharmacology
chemical_list: C473652:Hcar2 protein, mouse; D007657:Ketone Bodies; D043562:Receptors, G-Protein-Coupled; D011978:Receptors, Nicotinic; D014803:Vitamin B Complex; D009525:Niacin; D020155:3-Hydroxybutyric Acid
keywords: GPR109a; heat stress; hyperketonemia; niacin; β-OH butyrate