title: Antimicrobial cocktails to control bacterial and fungal contamination in Chlamydomonas reinhardtii cultures.
abstract: Chlamydomonas reinhardtii is a unicellular green alga widely used for research in photosynthesis, cell cycle regulation, ciliary biogenesis, and other physiological processes. Sterile cultures are needed for these studies, but contamination from bacteria and fungi occurs frequently. Although the One-shot Solution cocktail consisting of carbendazim, ampicillin, and cefotaxime has been developed for removing these contaminants from algal cultures, it is not always effective. Here we report two new antimicrobial cocktails for treating mixed bacterial and fungal contamination of Chlamydomonas cultures. A combination of the bactericide nalidixic acid with one of two fungicides, azoxystrobin or tebuconazole, was more effective than the One-shot Solution cocktail. In some of our tests, we find that alternating use of our new cocktails with One-shot Solution is needed to remove obstinate contaminants.
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, China.
authors.forename: Fengyuan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, China.
authors.forename: Huiyi
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, China.
authors.forename: Zhiyue
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, China.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, China.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D000935:Antifungal Agents / Q000008:administration & dosage*; D018929:Cell Culture Techniques / Q000379:methods*; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D004338:Drug Combinations
keywords: antimicrobial cocktail; bactericides; contamination; fungicides; microalgae