title: Taxonomic description and genome sequence of Salinicoccus sediminis sp. nov., a halotolerant bacterium isolated from marine sediment.
abstract: A Gram-staining-positive, coccoid, halotolerant bacterial strain, designated SV-16T, was isolated from marine sediment and subjected to a polyphasic taxonomic study. The strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Salinicoccus. Growth occurred at temperatures in the range 25-37 °C (optimum 30 °C), at pH 7.0-11.0 (optimum pH 8.0) and at NaCl concentrations of up to 25.0% (optimum 15.0%). The highest level of 16S rRNA gene sequence similarity was with Salinicoccus carnicancri CrmT (98.6%) followed by Salinicoccus halodurans W24T (96.6%). The predominant polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylglycerol. The major cellular fatty acids were iso-C15:0, anteiso-C15:0, iso-C17:0 and anteiso-C17:0. The draft genome of strain SV-16T consisted of 2,591,284 bp with a DNA G+C content of 48.7 mol%. On the basis of the phenotypic characteristics and genotypic distinctiveness of strain SV-16T, it should be classified within a novel species of the genus Salinicoccus, for which the name Salinicoccus sediminis sp. nov. is proposed. The type strain is SV-16T ( = MTCC 11832T = DSM 28797T).
authors.affiliation: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Rajendran Mathan
authors.initials: RM
authors.lastname: Kumar
authors.affiliation: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Gurwinder
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Division of protein science & Engineering, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Narender
authors.initials: N
authors.lastname: Kumar
authors.affiliation: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Anand
authors.initials: A
authors.lastname: Kumar
authors.affiliation: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Nitin Kumar
authors.initials: NK
authors.lastname: Singh
authors.affiliation: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Monu
authors.initials: M
authors.lastname: Bala
authors.affiliation: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Navjot
authors.initials: N
authors.lastname: Kaur
authors.affiliation: MTCC - Microbial Type Culture Collection & Gene Bank, CSIR - Institute of Microbial Technology, Chandigarh 160036, India.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Mayilraj
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S