title: Bioanalytical and instrumental screening of the uptake of sediment-borne, dioxin-like compounds in roach (Rutilus rutilus).
abstract: To examine the uptake of dioxin-like compounds (DLCs), common roaches (Rutilus rutilus) were exposed for 28 days to differently contaminated sediments from two major European rivers in a purpose-built facility. Dietary transfer of DLCs was investigated by exposing fish to sediments inoculated or non-inoculated with black worms (Lumbriculus variegatus). Dioxin-like polychlorinated biphenyls (DL-PCBs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), measured via high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) in sediments and whole fish, were used to calculate toxicity equivalent quotients (TEQs). TEQs were compared with biological toxicity equivalent quotients (BEQs) determined via the 7-ethoxyresorufin-O-deethylase (EROD) assay, performed with mammalian (H4IIE) and fish (RTL-W1) liver cell lines. TEQs and BEQs indicated an uptake of sediment-borne DLCs by roach, which was independent of sediment contamination levels, but rather reflected sediment-specific characteristics. For most sediment treatments, DLC uptake did not increase with time. Highest congener-specific uptake (DL-PCB 123) was 10-fold compared to control. Exposure to worm-inoculated sediment of highest overall DLC contamination caused a 2-fold (TEQ and H4IIE BEQ) greater uptake of DLCs by fish compared to the respective non-inoculated treatment. H4IIE cells showed the greatest sensitivity (0.37 ± 0.25 pM TCDD) and the strongest correlation with TEQs (r (2)  = 0.79), hence, they seem to be best suited for DLC screening of sediments and biota, amended by compound-specific instrumental analysis if required.
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Eichbaum
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Brinkmann
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.
authors.forename: Leonie
authors.initials: L
authors.lastname: Nuesser
authors.affiliation: Department G3: Biochemistry, Ecotoxicology, Federal Institute of Hydrology (BfG), Am Mainzer Tor 1, 56068, Koblenz, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Buchinger
authors.affiliation: Department G3: Biochemistry, Ecotoxicology, Federal Institute of Hydrology (BfG), Am Mainzer Tor 1, 56068, Koblenz, Germany.
authors.forename: Georg
authors.initials: G
authors.lastname: Reifferscheid
authors.affiliation: School of the Environment and Sustainability and Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3, Canada.
authors.forename: Garry
authors.initials: G
authors.lastname: Codling
authors.affiliation: School of the Environment and Sustainability and Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3, Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Jones
authors.affiliation: School of the Environment and Sustainability and Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3, Canada.|Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, SK, Canada.|School of Biological Sciences, University of Hong Kong, Hong Kong, SAR, China.|State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China.|Department of Biology, Hong Kong Baptist University, Hong Kong, SAR, People's Republic of China.
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.affiliation: School of the Environment and Sustainability and Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, S7N 5B3, Canada.
authors.forename: Markus
authors.initials: M
authors.lastname: Hecker
authors.affiliation: Department of Ecosystem Analysis, Institute for Environmental Research, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany. henner.hollert@bio5.rwth-aachen.de.|Key Laboratory of Yangtze River Environment of Education Ministry of China, College of Environmental Science and Engineering, Tongji University, Shanghai, China. henner.hollert@bio5.rwth-aachen.de.|College of Resources and Environmental Science, Chongqing University, Chongqing, China. henner.hollert@bio5.rwth-aachen.de.|School of Environment, Nanjing University, Nanjing, China. henner.hollert@bio5.rwth-aachen.de.
authors.forename: Henner
authors.initials: H
authors.lastname: Hollert
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D003530:Cyprinidae / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D000072318:Dibenzofurans / Q000493:pharmacokinetics* / Q000633:toxicity; D004032:Diet; D004147:Dioxins / Q000493:pharmacokinetics* / Q000633:toxicity; D019015:Geologic Sediments / Q000737:chemistry; D022781:Hepatocytes / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000493:pharmacokinetics* / Q000633:toxicity; D051381:Rats; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics* / Q000633:toxicity
chemical_list: D000072318:Dibenzofurans; D004147:Dioxins; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1
keywords: BEQ; Dioxin; EROD; Micro EROD