title: Use of Ozone to Treat Ileostomy Dermatitis in an Experimental Rat Model.
abstract: BACKGROUND
  Dermatitis associated with ileostomy is an important problem that affects many people, especially children. The aim of this study was to investigate the therapeutic effects of ozone on dermatitis due to ileostomy, and to develop an alternative treatment option.
  
  
  METHODS
  A total of 28 rats were divided into 4 groups: control, ileostomy, ozone, and zinc oxide. Ileostomy was performed in all rats except the control group. After a 1-week waiting time, the ozone group was administered ozone therapy and the zinc oxide group was administered zinc oxide cream locally once a day for a total of 7 days. All rats were sacrificed at the end of this period. The efficacy of treatment was examined by biochemical, histopathological, and immunohistochemical parameters. The levels of malondialdehyde (MDA), total glutathione (tGSH), total antioxidant capacity (TAC), and total oxidant status (TOS) were measured from tissue. Vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) were examined immunohistochemically.
  
  
  RESULTS
  Dermatitis occurred pathologically in all rats that underwent ileostomy surgery. The lowest dermatitis score was in the ozone treatment group (p<0.05). Ileostomy dermatitis caused increased levels of MDA and TOS. Ozone treatment resulted in reduced MDA and TOS levels, while the levels of tGSH and TAC were increased (p<0.05). Both VEGF and PCNA immunostaining were augmented in the ozone treatment group (p<0.05).
  
  
  CONCLUSIONS
  Local ozone application may be a good alternative compared to the conventional treatment methods for the prevention of skin lesions that develop after ileostomy.
authors.affiliation: Department of Pediatric Surgery, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
authors.forename: Şenol
authors.initials: Ş
authors.lastname: Biçer
authors.affiliation: Department of Pathology, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
authors.forename: İlyas
authors.initials: İ
authors.lastname: Sayar
authors.affiliation: Department of Physiology, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
authors.forename: Cebrail
authors.initials: C
authors.lastname: Gürsul
authors.affiliation: Department of General Surgery, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
authors.forename: Arda
authors.initials: A
authors.lastname: Işık
authors.affiliation: Department of Microbiology, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Aydın
authors.affiliation: Department of General Surgery, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Peker
authors.affiliation: Department of General Surgery, Erzincan University, Faculty of Medicine, Erzincan, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Demiryilmaz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003872:Dermatitis / Q000209:etiology* / Q000473:pathology / Q000628:therapy*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D007081:Ileostomy / Q000009:adverse effects*; D007150:Immunohistochemistry; D008315:Malondialdehyde / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D010126:Ozone / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D016877:Oxidants; D008315:Malondialdehyde; D010126:Ozone; D005978:Glutathione