title: A Further Investigation of the Effects of Extremely Low Frequency Magnetic Fields on Alkaline Phosphatase and Acetylcholinesterase.
abstract: Using a custom build spectrophotometer equipped with Helmholtz coils and designed to study the effects of magnetic fields on enzyme reactions in real-time we have investigated the influence of fields, from 100 μT to 10 mT and at a variety of field frequencies, on the membrane bound enzymes alkaline phosphatase and acetylcholinesterase. We have also employed other methods to apply a magnetic field, e.g. Biostim. In contrast to earlier reports we have been unable to detect any field effects on these enzymes under any field/frequency regime. We discuss possible reasons for the discrepancy between this and earlier work and note the particularly complex influence of small temperature changes that may confound analysis.
authors.affiliation: School of Biology, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, United Kingdom.
authors.forename: Gary
authors.initials: G
authors.lastname: Silkstone
authors.affiliation: School of Biology, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, United Kingdom.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Wilson
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D060526:Magnetic Fields; D013053:Spectrophotometry; D013379:Substrate Specificity
chemical_list: D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase