title: Eritadenine from Edible Mushrooms Inhibits Activity of Angiotensin Converting Enzyme in Vitro.
abstract: The inhibition of angiotensin converting enzyme (ACE) activity was determined in vitro by mushroom-derived eritadenine (EA), which was analyzed in 11 principal Korean edible mushrooms. EA inhibited ACE activity with 0.091 μM IC50, whereas the IC50 of captopril (CP), which is a reference compound, was 0.025 μM. Kinetic measurements of ACE reaction in the substrate of hippuryl-l-histidyl-l-leucine (HHL) with or without EA revealed that the Vmax (0.0465 O.D/30 min) was unchanged, but the the Km increased from 2.063 to 3.887 mM, indicating that EA competes with HHL for the active site. When EA was analyzed by HPLC, Lentinus edodes with a soft cap contained the highest amount EA (642.8 mg%); however, Phellinus linteus with a hard cap contained the least amount of EA (9.4 mg%). These results indicate that EA was a strong competitive inhibitor for ACE, and edible mushrooms with soft caps contained a significant amount of EA.
authors.affiliation: Division of Applied Life Sciences, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University , Jinju 660-701, Republic of Korea.
authors.forename: Sadia
authors.initials: S
authors.lastname: Afrin
authors.affiliation: Division of Applied Life Sciences, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University , Jinju 660-701, Republic of Korea.
authors.forename: Md Abdur
authors.initials: MA
authors.lastname: Rakib
authors.affiliation: Division of Applied Life Sciences, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University , Jinju 660-701, Republic of Korea.
authors.forename: Boh Hyun
authors.initials: BH
authors.lastname: Kim
authors.affiliation: HK Biotech. Co., Ltd. , Jinju 660-884, Republic of Korea.
authors.forename: Jeong Ok
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Division of Applied Life Sciences, Graduate School, and Institute of Agriculture & Life Science, Gyeongsang National University , Jinju 660-701, Republic of Korea.
authors.forename: Yeong Lae
authors.initials: YL
authors.lastname: Ha
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D001667:Binding, Competitive; D020134:Catalytic Domain; D007700:Kinetics; D009842:Oligopeptides / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D020942:Shiitake Mushrooms / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; C010980:hippuryl-histidyl-leucine; C100029:eritadenine; D007703:Peptidyl-Dipeptidase A; D000225:Adenine
keywords: IC50 value; angiotensin converting enzyme (ACE); competitive inhibition; edible mushrooms; eritadenine