title: Mutation of Glycosylation Sites in BST-2 Leads to Its Accumulation at Intracellular CD63-Positive Vesicles without Affecting Its Antiviral Activity against Multivesicular Body-Targeted HIV-1 and Hepatitis B Virus.
abstract: BST-2/tetherin blocks the release of various enveloped viruses including HIV-1 with a "physical tethering" model. The detailed contribution of N-linked glycosylation to this model is controversial. Here, we confirmed that mutation of glycosylation sites exerted an effect of post-translational mis-trafficking, leading to an accumulation of BST-2 at intracellular CD63-positive vesicles. BST-2 with this phenotype potently inhibited the release of multivesicular body-targeted HIV-1 and hepatitis B virus, without affecting the co-localization of BST-2 with EEA1 and LAMP1. These results suggest that N-linked glycosylation of human BST-2 is dispensable for intracellular virion retention and imply that this recently discovered intracellular tethering function may be evolutionarily distinguished from the canonical antiviral function of BST-2 by tethering nascent virions at the cell surface.
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun 130021, China. jully90@163.com.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Han
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun 130021, China. mingyulv@jlu.edu.cn.|Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China. mingyulv@jlu.edu.cn.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Lv
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun 130021, China. shiy707@nenu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun 130021, China. yjh-0-2002@hotmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Hepatology, First Hospital of Jilin University, Changchun 130021, China. niujunqi549b@sina.com.
authors.forename: Junqi
authors.initials: J
authors.lastname: Niu
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun 130021, China. yuxiaofang@jlu.edu.cn.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Yu
authors.affiliation: Institute of Virology and AIDS Research, First Hospital of Jilin University, Changchun 130021, China. zhangwenyan@jlu.edu.cn.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Zhang
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000276:immunology / Q000821:virology; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D015497:HIV-1 / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D022781:Hepatocytes / Q000276:immunology / Q000821:virology; D006801:Humans; D057146:Multivesicular Bodies / Q000737:chemistry / Q000276:immunology* / Q000821:virology*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D060149:Tetraspanin 30 / Q000032:analysis; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D015703:Antigens, CD; D058851:GPI-Linked Proteins; D050505:Mutant Proteins; D060149:Tetraspanin 30; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2; C554586:CD63 protein, human
keywords: BST-2; HBV; HIV-1; glycosylation