title: [Impact of the CFTR chloride channel on the cytoskeleton of mouse Sertoli cells].
abstract: OBJECTIVE
  To study the impact of the chloride channel dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR) on the cytoskeleton of Sertoli cells in the mouse.
  
  
  METHODS
  TM4 Sertoli cells were cultured and treated with CFTR(inh)-172 at the concentrations of 1, 5, 10 and 20 μmol/L for 48 hours. Then the cytotoxicity of CFT(inh)-172 was assessed by CCK-8 assay, the expressions of F-actin and Ac-tub in the TM4 Sertoli cells detected by immunofluorescence assay, and those of N-cadherin, vimentin and vinculin determined by qPCR.
  
  
  RESULTS
  CFTR(inh)-172 produced cytotoxicity to the TM4 Sertoli cells at the concentration of 20 μmol/L. The expressions of F-actin and Ac-tub were decreased gradually in the TM4 Sertoli cells with the prolonging of treatment time and increasing concentration of CFTR(inh)-172 (P < 0.05). The results of qPCR showed that different concentrations of CFTR(inh)-172 worked no significant influence on the mRNA expressions of N-cadherin, vimentin and vinculin in the Sertoli cells.
  
  
  CONCLUSIONS
  The CFTR chloride channel plays an important role in maintaining the normal cytoskeleton of Sertoli cells. The reduced function and expression of the CFTR chloride channel may affect the function of Sertoli cells and consequently spermatogenesis of the testis.
authors.forename: Hong-liang
authors.initials: HL
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Yu-chun
authors.initials: YC
authors.lastname: Gu
authors.forename: Kai
authors.initials: K
authors.lastname: Hong
authors.forename: Wen-hao
authors.initials: WH
authors.lastname: Tang
authors.forename: Lian-ming
authors.initials: LM
authors.lastname: Zhao
authors.forename: De-feng
authors.initials: DF
authors.lastname: Liu
authors.forename: Jia-ming
authors.initials: JM
authors.lastname: Mao
authors.forename: Yu-zhuo
authors.initials: YZ
authors.lastname: Yang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D018118:Chloride Channels / Q000502:physiology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000037:antagonists & inhibitors*; D003599:Cytoskeleton / Q000187:drug effects*; D008297:Male; D051379:Mice; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D013091:Spermatogenesis; D053778:Thiazolidines / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C482900:3-((3-trifluoromethyl)phenyl)-5-((3-carboxyphenyl)methylene)-2-thioxo-4-thiazolidinone; D000199:Actins; D001565:Benzoates; D018118:Chloride Channels; D053778:Thiazolidines; D019005:Cystic Fibrosis Transmembrane Conductance Regulator