title: Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.
abstract: Live cell-based SELEX (Systematic Evolution of Ligand EXponential enrichment) is a promising approach for identifying aptamers that can selectively bind to a cell-surface antigen or a particular target cell population. In particular, it offers a facile selection strategy for some special cell-surface proteins that are original glycosylated or heavily post-translationally modified, and are unavailable in their native/active conformation after in vitro expression and purification. In this chapter, we describe evolution of cell-type-specific RNA aptamers targeting the human CCR5 by combining the live cell-based SELEX strategy with high-throughput sequencing (HTS) and bioinformatics analysis.
authors.affiliation: Division of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 1500 East Duarte Rd, Duarte, CA, 91010, USA.
authors.forename: Jiehua
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Division of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 1500 East Duarte Rd, Duarte, CA, 91010, USA. jrossi@coh.org.|Irell and Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 1500 East Duarte Rd, Duarte, CA, 91010, USA. jrossi@coh.org.
authors.forename: John J
authors.initials: JJ
authors.lastname: Rossi
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics*; D002460:Cell Line; D002470:Cell Survival; D019295:Computational Biology / Q000379:methods; D055785:Gene Knockdown Techniques / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008024:Ligands; D019713:Receptors, CCR5 / Q000235:genetics*; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; C584933:CCR5 protein, human; D008024:Ligands; D019713:Receptors, CCR5
keywords: Aptamer-mediated targeted siRNA delivery; Cell-type-specific RNA aptamer; High-throughput sequencing (HTS); Human CCR5 protein; Live cell-based SELEX