title: Experimental Calcium Silicate-Based Cement with and without Zirconium Oxide Modulates Fibroblasts Viability.
abstract: The aim of this study was to verify whether the use of zirconium oxide as a radiopacifier of an experimental calcium silicate-based cement (WPCZO) leads to cytotoxicity. Fibroblasts were treated with different concentrations (10 mg/mL, 1 mg/mL, and 0.1 mg/mL) of the cements diluted in Dulbecco's modified Eagle's medium (DMEM) for periods of 12, 24, and 48 h. Groups tested were white Portland cement (WPC), white Portland cement with zirconium oxide (WPCZO), and white mineral trioxide aggregate Angelus (MTA). Control group cells were not treated. The cytotoxicity was evaluated through mitochondrial-activity (MTT) and cell-density (crystal violet) assays. All cements showed low cytotoxicity. In general, at the concentration of 10 mg/mL there was an increase in viability of those groups treated with WPC and WPCZO when compared to the control group (p<0.05). A similar profile for the absorbance values was noted among the groups: 10 mg/mL presented an increase in viability compared to the control group. On the other hand, smaller concentrations presented a similar or lower viability compared to the control group, in general. A new dental material composed of calcium silicate-based cement with 20% zirconium oxide as the radiopacifier showed low cytotoxicity as a promising material to be exploited for root-end filling.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, USP - Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Slompo
authors.affiliation: Centro de Ciências da Saúde, USC - Universidade do Sagrado Coração, Bauru, SP, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Peres-Buzalaf
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, USP - Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Kellen Cristina da Silva
authors.initials: KC
authors.lastname: Gasque
authors.affiliation: Department of Prosthesis and Periodontology, Bauru Dental School, USP - Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Carla Andreotti
authors.initials: CA
authors.lastname: Damante
authors.affiliation: Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, USP - Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Ronald
authors.initials: R
authors.lastname: Ordinola-Zapata
authors.affiliation: Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School, USP - Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Marco Antonio Hungaro
authors.initials: MA
authors.lastname: Duarte
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, USP - Universidade de São Paulo, Bauru, SP, Brazil.
authors.forename: Rodrigo Cardoso
authors.initials: RC
authors.lastname: de Oliveira
mesh_terms: D017610:Calcium Compounds / Q000008:administration & dosage*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D017640:Silicates / Q000008:administration & dosage*; D015040:Zirconium / Q000008:administration & dosage*
chemical_list: D017610:Calcium Compounds; D017640:Silicates; D015040:Zirconium; C028541:zirconium oxide; C031293:calcium silicate