title: Inhibitory effect of Pterocarpus indicus Willd water extract on IgE/Ag-induced mast cell and atopic dermatitis-like mouse models.
abstract: Pterocarpus indicus Willd has been widely used as a traditional medicine to treat edema, cancer, and hyperlipidemia, but its antiallergic properties and underlying mechanisms have not yet been studied. The purpose of this study was to evaluate the antiallergic activity of Pterocarpus indicus Willd water extract (PIW) using activated mast cells and an atopic dermatitis (AD)-like mouse model. PIW decreased IgE/Ag-induced mast cell degranulation and the phosphorylation of Syk and downstream signaling molecules such as PLC-γ, Akt, Erk 1/2, JNK compared to stimulated mast cells. In DNCB-induced AD-like mice, PIW reduced IgE level in serum, as well as AD-associated scratching behavior and skin severity score. These results indicate that PIW inhibits the allergic response by reducing mast cell activation and may have clinical potential as an antiallergic agent for disorders such as AD.
authors.affiliation: a Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Cha
authors.affiliation: a Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Wan-Joong
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: a Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Myung-Hun
authors.initials: MH
authors.lastname: Lee
authors.affiliation: a Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: a Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Seo Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: b Department of Biotechnology , College of Biomedical and Health Science, Konkuk University , Chungju , Korea.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
authors.affiliation: a Division of Biological Science and Technology , College of Science and Technology, Yonsei University , Wonju , Korea.
authors.forename: Tack-Joong
authors.initials: TJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D004137:Dinitrochlorobenzene; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000097:blood*; D007509:Irritants; D048670:MAP Kinase Kinase 4 / Q000235:genetics / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000276:immunology; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000276:immunology; D051966:Phospholipase C gamma / Q000235:genetics / Q000276:immunology; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000276:immunology; D035721:Pterocarpus / Q000737:chemistry*; D015398:Signal Transduction; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D000072377:Syk Kinase / Q000235:genetics / Q000276:immunology; D014867:Water
chemical_list: D018926:Anti-Allergic Agents; D004137:Dinitrochlorobenzene; D007509:Irritants; D010936:Plant Extracts; D014867:Water; D007073:Immunoglobulin E; D000072377:Syk Kinase; C000606897:Syk protein, mouse; D051057:Proto-Oncogene Proteins c-akt; C535148:Mapk1 protein, mouse; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048670:MAP Kinase Kinase 4; D051966:Phospholipase C gamma
keywords: Pterocarpus indicus Willd water extract; atopic dermatitis; mast cell