title: Cardiac Light-Sheet Fluorescent Microscopy for Multi-Scale and Rapid Imaging of Architecture and Function.
abstract: Light Sheet Fluorescence Microscopy (LSFM) enables multi-dimensional and multi-scale imaging via illuminating specimens with a separate thin sheet of laser. It allows rapid plane illumination for reduced photo-damage and superior axial resolution and contrast. We hereby demonstrate cardiac LSFM (c-LSFM) imaging to assess the functional architecture of zebrafish embryos with a retrospective cardiac synchronization algorithm for four-dimensional reconstruction (3-D space + time). By combining our approach with tissue clearing techniques, we reveal the entire cardiac structures and hypertrabeculation of adult zebrafish hearts in response to doxorubicin treatment. By integrating the resolution enhancement technique with c-LSFM to increase the resolving power under a large field-of-view, we demonstrate the use of low power objective to resolve the entire architecture of large-scale neonatal mouse hearts, revealing the helical orientation of individual myocardial fibers. Therefore, our c-LSFM imaging approach provides multi-scale visualization of architecture and function to drive cardiovascular research with translational implication in congenital heart diseases.
authors.affiliation: School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, China.|Department of Mechanical &Aerospace Engineering, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Fei
authors.affiliation: Department of Bioengineering, UCLA, Los Angeles, CA, USA.
authors.forename: Juhyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Bioengineering, UCLA, Los Angeles, CA, USA.|Department of Molecular, Cellular and Integrative Physiology, UCLA, Los Angeles, CA, USA.|Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.
authors.forename: René R Sevag
authors.initials: RR
authors.lastname: Packard
authors.affiliation: Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.
authors.forename: Konstantina-Ioanna
authors.initials: KI
authors.lastname: Sereti
authors.affiliation: Department of Electrical Engineering, University of Southern California, Los Angeles, CA, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Ma
authors.affiliation: Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.|Division of Cardiology, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA, USA.
authors.forename: Hanul
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Bioengineering, UCLA, Los Angeles, CA, USA.
authors.forename: Harrison
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Bioengineering, UCLA, Los Angeles, CA, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Sung
authors.affiliation: Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.
authors.forename: Rajan
authors.initials: R
authors.lastname: Kulkarni
authors.affiliation: Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.
authors.forename: Reza
authors.initials: R
authors.lastname: Ardehali
authors.affiliation: Department of Electrical Engineering, University of Southern California, Los Angeles, CA, USA.
authors.forename: C-C Jay
authors.initials: CC
authors.lastname: Kuo
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Mechanical &Aerospace Engineering, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.
authors.forename: Chih-Ming
authors.initials: CM
authors.lastname: Ho
authors.affiliation: Department of Bioengineering, UCLA, Los Angeles, CA, USA.|Department of Molecular, Cellular and Integrative Physiology, UCLA, Los Angeles, CA, USA.|Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.|Division of Cardiology, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA, USA.
authors.forename: Tzung K
authors.initials: TK
authors.lastname: Hsiai
mesh_terms: D000465:Algorithms; D000818:Animals; D030801:Animals, Genetically Modified; D004317:Doxorubicin / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D004317:Doxorubicin