title: Serotypes and Clonal Diversity of Streptococcus pneumoniae Causing Invasive Disease in the Era of PCV13 in Catalonia, Spain.
abstract: The aim of this study was to study the serotypes and clonal diversity of pneumococci causing invasive pneumococcal disease in Catalonia, Spain, in the era of 13-valent pneumococcal conjugate vaccine (PCV13). In our region, this vaccine is only available in the private market and it is estimated a PCV13 vaccine coverage around 55% in children. A total of 1551 pneumococcal invasive isolates received between 2010 and 2013 in the Molecular Microbiology Department at Hospital Sant Joan de Déu, Barcelona, were included. Fifty-two serotypes and 249 clonal types-defined by MLST-were identified. The most common serotypes were serotype 1 (n = 182; 11.7%), 3 (n = 145; 9.3%), 19A (n = 137; 8.8%) and 7F (n = 122; 7.9%). Serotype 14 was the third most frequent serotype in children < 2 years (15 of 159 isolates). PCV7 serotypes maintained their proportion along the period of study, 16.6% in 2010 to 13.4% in 2013, whereas there was a significant proportional decrease in PCV13 serotypes, 65.3% in 2010 to 48.9% in 2013 (p<0.01). This decrease was mainly attributable to serotypes 19A and 7F. Serotype 12F achieved the third position in 2013 (n = 22, 6.4%). The most frequent clonal types found were ST306 (n = 154, 9.9%), ST191 (n = 111, 7.2%), ST989 (n = 85, 5.5%) and ST180 (n = 80, 5.2%). Despite their decrease, PCV13 serotypes continue to be a major cause of disease in Spain. These results emphasize the need for complete PCV13 vaccination.
authors.affiliation: Department of Molecular Microbiology, Hospital Sant Joan de Deu, Esplugues de Llobregat, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Del Amo
authors.affiliation: Department of Molecular Microbiology, Hospital Sant Joan de Deu, Esplugues de Llobregat, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Esteva
authors.affiliation: Department of Paediatrics, Hospital Sant Joan de Deu and University of Barcelona, Esplugues de Llobregat, Spain.
authors.forename: Susanna
authors.initials: S
authors.lastname: Hernandez-Bou
authors.affiliation: Department of Microbiology, Hospital Sant Jaume, Calella, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Galles
authors.affiliation: Department of Microbiology, Hospital de Vic, Vic. Spain.
authors.forename: Marian
authors.initials: M
authors.lastname: Navarro
authors.affiliation: Department of Microbiology, Hospital de Mataró, Mataró, Spain.
authors.forename: Goretti
authors.initials: G
authors.lastname: Sauca
authors.affiliation: Department of Microbiology, Hospital de Nens, Barcelona, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Diaz
authors.affiliation: Department of Microbiology, Hospital de Figueres, Figueres, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Gassiot
authors.affiliation: Department of Microbiology, Hospital General de Granollers, Granollers, Spain.
authors.forename: Carmina
authors.initials: C
authors.lastname: Marti
authors.affiliation: Department of Microbiology, Hospital Vall d'Hebron, Barcelona, Spain.
authors.forename: Nieves
authors.initials: N
authors.lastname: Larrosa
authors.affiliation: Public Health Agency, Government of Catalonia, Barcelona, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Ciruela
authors.affiliation: Public Health Agency, Government of Catalonia, Barcelona, Spain.
authors.forename: Mireia
authors.initials: M
authors.lastname: Jane
authors.affiliation: Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
authors.forename: Raquel
authors.initials: R
authors.lastname: Sá-Leão
authors.affiliation: Department of Molecular Microbiology, Hospital Sant Joan de Deu, Esplugues de Llobregat, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Muñoz-Almagro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D058885:Multilocus Sequence Typing; D011008:Pneumococcal Infections / Q000453:epidemiology* / Q000382:microbiology* / Q000517:prevention & control; D022242:Pneumococcal Vaccines / Q000276:immunology; D065288:Serogroup; D013030:Spain / Q000453:epidemiology; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D055815:Young Adult
chemical_list: C538862:13-valent pneumococcal vaccine; D000900:Anti-Bacterial Agents; D022242:Pneumococcal Vaccines