title: Visualizing fungal metabolites during mycoparasitic interaction by MALDI mass spectrometry imaging.
abstract: Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home-built sublimation device and thorough recrystallization. We present for the first time MALDI MSI data for secondary metabolite release during active mycoparasitism.
authors.affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Holzlechner
authors.affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.
authors.forename: Sonja
authors.initials: S
authors.lastname: Reitschmidt
authors.affiliation: Institute of Microbiology, University of Innsbruck, Innsbruck, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Gruber
authors.affiliation: Institute of Microbiology, University of Innsbruck, Innsbruck, Austria.|Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria.
authors.forename: Susanne
authors.initials: S
authors.lastname: Zeilinger
authors.affiliation: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Marchetti-Deschmann
mesh_terms: D015966:Gene Expression Regulation, Fungal; D056265:Microbial Interactions; D012232:Rhizoctonia / Q000737:chemistry / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014242:Trichoderma / Q000737:chemistry / Q000378:metabolism*
keywords: Fungal communication; MALDI MSI; Mycoparasitism; Peptaibiotics; Rhizoctonia; Trichoderma