title: [Hydroxysafflor yellow A inhibits rat vascular smooth muscle cells proliferation possibly via blocking signal transduction of MEK-ERK1/2].
abstract: OBJECTIVE
  To elucidate the effect of hydroxysafflor yellow A ( HYSA) on the proliferation of vascular smooth muscle cells (VSMCs) and the related mechanism.
  
  
  METHODS
  VSMCs derived from SD rats were treated with DMEC culture medium (Control), 10 ng/ml PDGF (PDGF group), pretreatment with HYSA at different doses (1, 5, 10, 20, 40, 60 µmol/L) for 24 h then cotreatment with PDGF. After 24 h, MTT assay, Western blot and immunohistochemical staining were performed to evaluate the inhibitory effects of HYSA on VSMCs proliferation.
  
  
  RESULTS
  HYSA inhibited PDGF induced VSMCs proliferation in a dose-dependent manner, dowregulated proliferating cell nuclear antigen (PCNA) expression and blocked PDGF activated PDGFR-MEK-ERK1/2 signaling pathway.
  
  
  CONCLUSIONS
  HYSA inhibits VSMCs proliferation possibly via downregulating the expression of PCNA and blocking MEK-ERK1/2 signal transduction in VSMCs.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jingshan
authors.forename: Guo
authors.initials: G
authors.lastname: Qianyu
authors.forename: Lai
authors.initials: L
authors.lastname: Shaohong
authors.forename: Zhang
authors.initials: Z
authors.lastname: Shangyue
authors.forename: Han
authors.initials: H
authors.lastname: Hongli
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D002599:Chalcone / Q000031:analogs & derivatives; D020935:MAP Kinase Signaling System; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D009131:Muscle, Smooth, Vascular; D032389:Myocytes, Smooth Muscle; D018809:Proliferating Cell Nuclear Antigen; D011809:Quinones; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D011809:Quinones; C085278:hydroxysafflor yellow A; D002599:Chalcone; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases