title: Enhanced bone formation in electrospun poly(L-lactic-co-glycolic acid)-tussah silk fibroin ultrafine nanofiber scaffolds incorporated with graphene oxide.
abstract: To engineer bone tissue, it is necessary to provide a biocompatible, mechanically robust scaffold. In this study, we fabricated an ultrafine nanofiber scaffold by electrospinning a blend of poly(L-lactic-co-glycolic acid), tussah silk fibroin, and graphene oxide (GO) and characterized its morphology, biocompatibility, mechanical properties, and biological activity. The data indicate that incorporation of 10 wt.% tussah silk and 1 wt.% graphene oxide into poly(L-lactic-co-glycolic acid) nanofibers significantly decreased the fiber diameter from 280 to 130 nm. Furthermore, tussah silk and graphene oxide boosted the Young's modulus and tensile strength by nearly 4-fold and 3-fold, respectively, and significantly enhanced adhesion, proliferation in mouse mesenchymal stem cells and functionally promoted biomineralization-relevant alkaline phosphatase (ALP) and mineral deposition. The results indicate that composite nanofibers could be excellent and versatile scaffolds for bone tissue engineering.
authors.affiliation: Key Laboratory of Advanced Textile Composites (Ministry of Education), Institute of Textile Composites, Tianjin Polytechnic University, Tianjin 300387, China; Henan Provincial Key Laboratory of Functional Textile Materials, Zhongyuan University of Technology, Zhengzhou 450007, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Shao
authors.affiliation: Henan Provincial Key Laboratory of Functional Textile Materials, Zhongyuan University of Technology, Zhengzhou 450007, China. Electronic address: hejianxin771117@163.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Acquired Immune Deficiency Syndrome Treatment and Research Center, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sang
authors.affiliation: Henan Provincial Key Laboratory of Functional Textile Materials, Zhongyuan University of Technology, Zhengzhou 450007, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Advanced Textile Composites (Ministry of Education), Institute of Textile Composites, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Advanced Textile Composites (Ministry of Education), Institute of Textile Composites, Tianjin Polytechnic University, Tianjin 300387, China; Henan Provincial Key Laboratory of Functional Textile Materials, Zhongyuan University of Technology, Zhengzhou 450007, China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Cui
authors.affiliation: Henan Provincial Key Laboratory of Functional Textile Materials, Zhongyuan University of Technology, Zhengzhou 450007, China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201600, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ding
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001842:Bone and Bones / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D055119:Elastic Modulus; D005349:Fibroins / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects; D010087:Oxides / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D010087:Oxides; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006108:Graphite; D005349:Fibroins; D000469:Alkaline Phosphatase
keywords: Biocompatibility; Bone tissue engineering; Graphene oxide; Osteogenesis; Poly(l-lactic-co-glycolic acid); Tussah silk