title: In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone.
abstract: Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Queensland, Australia.
authors.forename: Pablo J
authors.initials: PJ
authors.lastname: Sanchís-Benlloch
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Queensland, Australia.
authors.forename: Josephine
authors.initials: J
authors.lastname: Nocillado
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Queensland, Australia.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ladisa
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Queensland, Australia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Aizen
authors.affiliation: Clean Seas Tuna Ltd, Port Lincoln, SA, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: Miller
authors.affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Shpilman
authors.affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
authors.forename: Berta
authors.initials: B
authors.lastname: Levavi-Sivan
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Queensland, Australia.
authors.forename: Tomer
authors.initials: T
authors.lastname: Ventura
authors.affiliation: Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Queensland, Australia. Electronic address: AElizur@usc.edu.au.
authors.forename: Abigail
authors.initials: A
authors.lastname: Elizur
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood* / Q000494:pharmacology*; D008297:Male; D009866:Oogenesis / Q000187:drug effects*; D010473:Perciformes / Q000097:blood / Q000502:physiology*; D010843:Pichia; D011994:Recombinant Proteins / Q000494:pharmacology*; D012741:Sexual Maturation / Q000187:drug effects*
chemical_list: D011994:Recombinant Proteins; D004958:Estradiol; D005640:Follicle Stimulating Hormone
keywords: FSH; Pichia pastoris; Recombinant protein; Seriola lalandi; Teleost puberty