title: Body composition of the host influences dendritic cell phenotype in patients treated for colorectal cancer.
abstract: Dendritic cells (DCs) are antigen-presenting cells that can acquire tumour antigens and initiate cytotoxic T cell reactions. Obesity has been proposed as a cause for tumours escaping immune surveillance, but few studies investigate the impact of other body composition parameters. We examined the relationship of DC phenotype with computer tomography (CT)-defined parameters in patients with colorectal cancer (CRC). DCs were identified within peripheral blood mononuclear cells by flow cytometry as HLA-DR positive and negative for markers of other cell lineages in 21 patients. Analysis of CT scans was used to calculate lumbar skeletal muscle index (LSMI) and mean muscle attenuation (MA). Positive correlation between the LSMI and expression of CD40 in all DCs (r = 0.45; p = 0.04) was demonstrated. The MA was positively correlated with scavenger receptor CD36 [all DCs (r = 0.60; p = 0.01) and myeloid DCs (r = 0.63; p < 0.01)]. However, the MA was negatively correlated with CCR7 expression in all DCs (r = -0.46, p = 0.03.) and with CD83 [all DCs (r = -0.63; p = 0.01) and myeloid DCs (r = -0.75; p < 0.01)]. There were no relationships between the fat indexes and the DC phenotype. These results highlight a direct relationship between muscle depletion and changes in stimulatory, migratory and fatty acid-processing potential of DC in patients with CRC.
authors.affiliation: Antigen Presentation Research Group, Imperial College London, North West London Hospitals Campus, Watford Road, Harrow, HA1 3UJ, UK.|Department of Surgery, St. Mark's Hospital, Watford Road, Harrow Middlesex, HA1 3UJ, UK.
authors.forename: George
authors.initials: G
authors.lastname: Malietzis
authors.affiliation: Antigen Presentation Research Group, Imperial College London, North West London Hospitals Campus, Watford Road, Harrow, HA1 3UJ, UK.|Department of Surgery, St. Mark's Hospital, Watford Road, Harrow Middlesex, HA1 3UJ, UK.
authors.forename: Gui Han
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Antigen Presentation Research Group, Imperial College London, North West London Hospitals Campus, Watford Road, Harrow, HA1 3UJ, UK.
authors.forename: Hafid O
authors.initials: HO
authors.lastname: Al-Hassi
authors.affiliation: Antigen Presentation Research Group, Imperial College London, North West London Hospitals Campus, Watford Road, Harrow, HA1 3UJ, UK.|Gastroenterology Unit, Hospital Universitario de La Princesa and Instituto de Investigación Sanitaria Princesa (IIS-IP), Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Madrid, 28006, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Bernardo
authors.affiliation: Section of Investigative Medicine, Division of Diabetes, Endocrinology, and Metabolism, Faculty of Medicine|, Imperial College, London, W12 0NN, UK.|Department of Life Sciences, Brunel University London, Middlesex, UB8 3PH, UK.
authors.forename: Alexandra I F
authors.initials: AI
authors.lastname: Blakemore
authors.affiliation: Department of Surgery, St. Mark's Hospital, Watford Road, Harrow Middlesex, HA1 3UJ, UK.
authors.forename: Robin H
authors.initials: RH
authors.lastname: Kennedy
authors.affiliation: Department of Histopathology, St. Mark's Hospital, Watford Road, Harrow Middlesex, HA1 3UJ, UK.
authors.forename: Morgan
authors.initials: M
authors.lastname: Moorghen
authors.affiliation: Department of Surgery, St. Mark's Hospital, Watford Road, Harrow Middlesex, HA1 3UJ, UK.
authors.forename: John T
authors.initials: JT
authors.lastname: Jenkins
authors.affiliation: Antigen Presentation Research Group, Imperial College London, North West London Hospitals Campus, Watford Road, Harrow, HA1 3UJ, UK. s.knight@imperial.ac.uk.
authors.forename: Stella C
authors.initials: SC
authors.lastname: Knight
mesh_terms: D000328:Adult; D000368:Aged; D001823:Body Composition / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D014057:Tomography, X-Ray Computed
keywords: Body composition; Colorectal cancer; Dendritic cells; Myopenia