title: Supramolecular self-assembly of graphene oxide and metal nanoparticles into stacked multilayers by means of a multitasking protein ring.
abstract: Graphene oxide (GO) is rapidly emerging worldwide as a breakthrough precursor material for next-generation devices. However, this requires the transition of its two-dimensional layered structure into more accessible three-dimensional (3D) arrays. Peroxiredoxins (Prx) are a family of multitasking redox enzymes, self-assembling into ring-like architectures. Taking advantage of both their symmetric structure and function, 3D reduced GO-based composites are hereby built up. Results reveal that the "double-faced" Prx rings can adhere flat on single GO layers and partially reduce them by their sulfur-containing amino acids, driving their stacking into 3D multi-layer reduced GO-Prx composites. This process occurs in aqueous solution at a very low GO concentration, i.e. 0.2 mg ml(-1). Further, protein engineering allows the Prx ring to be enriched with metal binding sites inside its lumen. This feature is exploited to both capture presynthesized gold nanoparticles and grow in situ palladium nanoparticles paving the way to straightforward and "green" routes to 3D reduced GO-metal composite materials.
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ardini
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Giordana
authors.initials: G
authors.lastname: Golia
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Passaretti
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Cimini
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Pitari
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Giansanti
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Luana
authors.initials: L
authors.lastname: Di Leandro
authors.affiliation: Dept. of Physics and Chemistry, University of L'Aquila, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Ottaviano
authors.affiliation: Dept. of Physics and Chemistry, University of L'Aquila, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Perrozzi
authors.affiliation: Dept. of Physics and Chemistry, University of L'Aquila, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: Santucci
authors.affiliation: National Research Council of Italy, Institute for Microelectronics and Microsystems, Bologna, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Morandi
authors.affiliation: National Research Council of Italy, Institute for Microelectronics and Microsystems, Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Ortolani
authors.affiliation: National Research Council of Italy, Institute for Microelectronics and Microsystems, Bologna, Italy and National Research Council of Italy, Institute for Organic Synthesis and Photoelectronics, Bologna, Italy.
authors.forename: Meganne
authors.initials: M
authors.lastname: Christian
authors.affiliation: National Research Council of Italy, Institute for Organic Synthesis and Photoelectronics, Bologna, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Treossi
authors.affiliation: National Research Council of Italy, Institute for Organic Synthesis and Photoelectronics, Bologna, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Palermo
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Angelucci
authors.affiliation: Dept. of Life, Health and Environmental Sciences, University of L'Aquila, Italy. rodolfo.ippoliti@univaq.it francesco.angelucci@univaq.it.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Ippoliti
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D003545:Cysteine / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D053768:Metal Nanoparticles / Q000737:chemistry*; D008715:Methionine / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D017348:Microscopy, Electron, Scanning Transmission; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D054464:Peroxiredoxins / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D012550:Schistosoma mansoni; D013056:Spectrophotometry, Ultraviolet; D013455:Sulfur / Q000737:chemistry; D013696:Temperature; D013864:Thioglycolates / Q000737:chemistry
chemical_list: D000596:Amino Acids; D007477:Ions; D010087:Oxides; D013864:Thioglycolates; D010165:Palladium; D013455:Sulfur; D006046:Gold; D006108:Graphite; C017487:2-mercaptoacetate; D008715:Methionine; D054464:Peroxiredoxins; D003545:Cysteine