title: Characterization of a novel qepA3 variant in Enterobacter aerogenes.
abstract: Five isolates harboring qepA were studied by polymerase chain reaction (PCR) amplification and relevant methods. One was determined to be a novel qepA3 from Enterobacter aerogenes, and four involved three qepA1 and one qepA2 determinants from Escherichia coli; the qepA3 changed five amino acids. These results characterized genetic structures A, B, C, D, and E.
authors.affiliation: Department of Clinical Laboratories for Infectious Disease, Taizhou Municipal Hospital, Zhejiang, China; Institute of Molecular Diagnostics of Taizhou University, Zhejiang, China. Electronic address: wdgtzs@163.com.
authors.forename: Dongguo
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Infectious Disease, Wenling People's Hospital, Zhejiang, China. Electronic address: hxt2380@163.com.
authors.forename: Xitian
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, Medical College of Taizhou University, Zhejiang, China; Institute of Molecular Diagnostics of Taizhou University, Zhejiang, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, Taizhou Municipal Hospital, Zhejiang, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Mou
authors.affiliation: Department of Laboratory Medicine, Medical College of Taizhou University, Zhejiang, China.
authors.forename: Yongxiao
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000235:genetics*; D016972:Enterobacter cloacae / Q000187:drug effects / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005798:Genes, Bacterial; D040342:Genetic Structures; D008826:Microbial Sensitivity Tests; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D015363:Quinolones / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015363:Quinolones
keywords: Enterobacter aerogenes; Escherichia coli; genetic structure; novel qepA3 variant gene; quinolone resistance