title: MreB-Dependent Organization of the E. coli Cytoplasmic Membrane Controls Membrane Protein Diffusion.
abstract: The functional organization of prokaryotic cell membranes, which is essential for many cellular processes, has been challenging to analyze due to the small size and nonflat geometry of bacterial cells. Here, we use single-molecule fluorescence microscopy and three-dimensional quantitative analyses in live Escherichia coli to demonstrate that its cytoplasmic membrane contains microdomains with distinct physical properties. We show that the stability of these microdomains depends on the integrity of the MreB cytoskeletal network underneath the membrane. We explore how the interplay between cytoskeleton and membrane affects trans-membrane protein (TMP) diffusion and reveal that the mobility of the TMPs tested is subdiffusive, most likely caused by confinement of TMP mobility by the submembranous MreB network. Our findings demonstrate that the dynamic architecture of prokaryotic cell membranes is controlled by the MreB cytoskeleton and regulates the mobility of TMPs.
authors.affiliation: Department of Physics and Astronomy, Vrije Universiteit Amsterdam, the Netherlands; Department of Molecular Cell Biology, Vrije Universiteit Amsterdam, the Netherlands; LaserLaB Amsterdam, the Netherlands.
authors.forename: Felix
authors.initials: F
authors.lastname: Oswald
authors.affiliation: Department of Physics and Astronomy, Vrije Universiteit Amsterdam, the Netherlands; LaserLaB Amsterdam, the Netherlands.
authors.forename: Aravindan
authors.initials: A
authors.lastname: Varadarajan
authors.affiliation: Department of Molecular Cell Biology, Vrije Universiteit Amsterdam, the Netherlands; LaserLaB Amsterdam, the Netherlands.
authors.forename: Holger
authors.initials: H
authors.lastname: Lill
authors.affiliation: Department of Physics and Astronomy, Vrije Universiteit Amsterdam, the Netherlands; LaserLaB Amsterdam, the Netherlands.
authors.forename: Erwin J G
authors.initials: EJ
authors.lastname: Peterman
authors.affiliation: Department of Molecular Cell Biology, Vrije Universiteit Amsterdam, the Netherlands; LaserLaB Amsterdam, the Netherlands. Electronic address: yves.bollen@vu.nl.
authors.forename: Yves J M
authors.initials: YJ
authors.lastname: Bollen
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D058105:Polymerization
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C078473:MreB protein, E coli