title: The interferon-inducible protein p205 acts as an activator in osteoblast differentiation of mouse BMSCs.
abstract: p205, an interferon-inducible protein, is induced in the course of osteogenesis in mouse bone marrow stromal cells (BMSCs). Knocking down p205 markedly impairs whereas overexpressing p205 enhances the osteoblast differentiation of BMSCs, as revealed by the altered expression of osteogenic genes, the change of ALP activity and the ARS-stained mineral nodules. The positive role of p205 in BMSC osteogenesis is probably due, at least in part, to the association of it with Id proteins. Further investigation indicated that p205 may disturb the formation of Runx2/Ids complex and free more Runx2 to induce the differentiation process. Taken together, our findings demonstrated for the first time that p205 functions as an activator in osteoblast differentiation.
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China. Electronic address: hfli1228@163.com.
authors.forename: Haifang
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Xianning
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
authors.forename: Hengjun
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Laboratory, 88th Hospital of PLA, Tai'an 271000, China.
authors.forename: Huibin
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics*; D007372:Interferons / Q000008:administration & dosage; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D009479:Neuropeptides / Q000235:genetics*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000235:genetics*; D000075370:Receptors for Activated C Kinase
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D009479:Neuropeptides; C090294:RACK1 protein, mouse; D000075370:Receptors for Activated C Kinase; C493716:Runx2 protein, mouse; D007372:Interferons
keywords: Bone marrow stromal cells (BMSCs); Id proteins; Interferon-inducible protein p205; Osteoblast differentiation; Runx2