title: Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells.
abstract: Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment.
authors.affiliation: Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele Hospital, Milano, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Mezzapelle
authors.affiliation: Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele Hospital, Milano, Italy.
authors.forename: Eltjona
authors.initials: E
authors.lastname: Rrapaj
authors.affiliation: Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele Hospital, Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Gatti
authors.affiliation: Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele Hospital, Milano, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Ceriotti
authors.affiliation: Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele Hospital, Milano, Italy.
authors.forename: Francesco De
authors.initials: FD
authors.lastname: Marchis
authors.affiliation: HMGBiotech, Milano, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Preti
authors.affiliation: Experimental Imaging Center, San Raffaele Hospital, Milano, Italy.|Medical Physics Unit, San Raffaele Hospital, Milano, Italy.
authors.forename: Antonello E
authors.initials: AE
authors.lastname: Spinelli
authors.affiliation: Experimental Imaging Center, San Raffaele Hospital, Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Perani
authors.affiliation: Experimental Imaging Center, San Raffaele Hospital, Milano, Italy.|Diagnostic Radiology Unit, San Raffaele Hospital, Milano, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Venturini
authors.affiliation: Experimental Imaging Center, San Raffaele Hospital, Milano, Italy.|IBFM-CNR, Segrate, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Valtorta
authors.affiliation: Experimental Imaging Center, San Raffaele Hospital, Milano, Italy.|Health Sciences Dept., Milano Bicocca University, Milano, Italy.
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Moresco
authors.affiliation: Pathological Anatomy Laboratory, San Raffaele Hospital, Milano, Italy.
authors.forename: Lorenza
authors.initials: L
authors.lastname: Pecciarini
authors.affiliation: Pathological Anatomy Laboratory, San Raffaele Hospital, Milano, Italy.|San Raffaele Vita-Salute University, Milano, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Doglioni
authors.affiliation: Molecular Oncology Unit, San Raffaele Hospital, Milano, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Frenquelli
authors.affiliation: ISTOVET, Besana in Brianza, Monza e Brianza, Italy;
authors.forename: Luca
authors.initials: L
authors.lastname: Crippa
authors.affiliation: Fondazione Filarete, Milano, Italy.
authors.forename: Camilla
authors.initials: C
authors.lastname: Recordati
authors.affiliation: Fondazione Filarete, Milano, Italy.|Università degli Studi, Milano, Italy.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Scanziani
authors.affiliation: Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
authors.forename: Hilda
authors.initials: H
authors.lastname: de Vries
authors.affiliation: Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
authors.forename: Anton
authors.initials: A
authors.lastname: Berns
authors.affiliation: Mario Negri Institute, Milano, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Frapolli
authors.affiliation: Division of Pathology "Maggiore Della Carità" Hospital, Novara, Italy.
authors.forename: Renzo
authors.initials: R
authors.lastname: Boldorini
authors.affiliation: Mario Negri Institute, Milano, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: D'Incalci
authors.affiliation: Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele Hospital, Milano, Italy.|San Raffaele Vita-Salute University, Milano, Italy.
authors.forename: Marco E
authors.initials: ME
authors.lastname: Bianchi
authors.affiliation: Chromatin Dynamics Unit, Division of Genetics and Cell Biology, San Raffaele Hospital, Milano, Italy.
authors.forename: Massimo P
authors.initials: MP
authors.lastname: Crippa
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000627:therapeutic use; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000627:therapeutic use; D005260:Female; D024243:HMGB1 Protein / Q000378:metabolism; D006801:Humans; D007121:Immunocompetence; D008175:Lung Neoplasms / Q000098:blood supply / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D008654:Mesothelioma / Q000098:blood supply / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D000086002:Mesothelioma, Malignant; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D000068437:Pemetrexed / Q000627:therapeutic use; D016019:Survival Analysis; D000093542:Gemcitabine
chemical_list: D000970:Antineoplastic Agents; D024243:HMGB1 Protein; D000068437:Pemetrexed; D003841:Deoxycytidine; D002945:Cisplatin; D000093542:Gemcitabine