title: Expression of lactate dehydrogenase C in MDA‑MB‑231 cells and its role in tumor invasion and migration.
abstract: The cancer/testis antigen (CTA) lactate dehydrogenase C (LDHC) is a unique LDH isoenzyme associated with glucose and adenosine triphosphate production in mammalian germ cells. However, the role of LDHC in cancer has thus far largely remained elusive. The present study described the expression status of LDHC in human MDA‑MB‑231 breast cancer cells as well as its role in tumor invasion and migration. Immunohistochemical analysis revealed endogenous LDHC expression in the cytoplasm and nuclei of MDA‑MB‑231 cells yielded. In addition, in vitro cell invasion and migration assays revealed that when LDHC expression was blocked by its specific inhibitor, cell invasion and migration were compromised in MDA‑MB‑231 cells. Of note, inhibition of LDHC was unable to induce apoptosis in MDA‑MB‑231 cells. The present study provided evidence that the LDHC enzyme acts as a CTA in breast carcinoma and exerts an essential role in tumor invasion and migration.
authors.affiliation: Department of Pathology, Fujian University of Traditional Chinese Medicine Affiliated People's Hospital, Taijiang, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Lingying
authors.initials: L
authors.lastname: Kong
authors.affiliation: Department of Pathology, Fujian University of Traditional Chinese Medicine Affiliated People's Hospital, Taijiang, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: Department of Clinical Laboratory, School of Medical Technology and Engineering, Fujian Medical University, Taijiang, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Zhaolei
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Pathology, Fujian University of Traditional Chinese Medicine Affiliated People's Hospital, Taijiang, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pathology, Fujian University of Traditional Chinese Medicine Affiliated People's Hospital, Taijiang, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Pathology, Fujian University of Traditional Chinese Medicine Affiliated People's Hospital, Taijiang, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, School of Medical Technology and Engineering, Fujian Medical University, Taijiang, Fuzhou, Fujian 350004, P.R. China.
authors.forename: Donghong
authors.initials: D
authors.lastname: Lin
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D061986:MCF-7 Cells
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; C409673:lactate dehydrogenase C4