title: Identification and validation of a virus-inducible ta-siRNA-generating TAS4 locus in tomato.
abstract: Trans-acting small interfering RNAs (ta-siRNAs) are a class of endogenous small RNA, associated with posttranscriptional gene silencing. Their biogenesis requires an initial microRNA (miRNA)-mediated cleavage of precursor RNA. Around 20 different ta-siRNA-producing loci (TASs), whose sequences are conserved, are reported in plants. In tomato, two TAS gene families have been identified, which are found to target auxin response factor gene and bacterial spot disease resistance protein Bs4 gene. Using high-throughput computational and experimental approach, we identified a new locus-producing ta-siRNA in tomato. We have also identified the putative miRNA regulating the production of ta-siRNA from this locus. The ta-siRNAs generated from TAS4 were up-regulated upon infection with a DNA virus. The potential targets of ta-siRNAs were predicted to be variety of proteins including MYB transcription factors and cell cycle regulators for some of the ta-siRNAs produced.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Singh
authors.forename: Shradha
authors.initials: S
authors.lastname: Saraf
authors.forename: Indranil
authors.initials: I
authors.lastname: Dasgupta
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Mukherjee
mesh_terms: D060467:Disease Resistance / Q000235:genetics*; D017791:Geminiviridae / Q000235:genetics / Q000472:pathogenicity; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000821:virology; D035683:MicroRNAs / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D012322:RNA Precursors / Q000235:genetics; D034741:RNA, Small Interfering / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D007210:Indoleacetic Acids; D035683:MicroRNAs; D012322:RNA Precursors; D034741:RNA, Small Interfering