title: Cyclosporin A significantly improves preeclampsia signs and suppresses inflammation in a rat model.
abstract: Preeclampsia is associated with an increased inflammatory response. Immune suppression might be an effective treatment. The aim of this study was to examine whether Cyclosporin A (CsA), an immunosuppressant, improves clinical characteristics of preeclampsia and suppresses inflammation in a lipopolysaccharide (LPS) induced preeclampsia rat model. Pregnant rats were randomly divided into 4 groups: group 1 (PE) rats each received LPS via tail vein on gestational day (GD) 14; group 2 (PE+CsA5) rats were pretreated with LPS (1.0 μg/kg) on GD 14 and were then treated with CsA (5mg/kg, ip) on GDs 16, 17 and 18; group 3 (PE+CsA10) rats were pretreated with LPS (1.0 μg/kg) on GD 14 and were then treated with CsA (10mg/kg, ip) on GDs 16, 17 and 18; group 4 (pregnant control, PC) rats were treated with the vehicle (saline) used for groups 1, 2 and 3. Systolic blood pressure, urinary albumin, biometric parameters and the levels of serum cytokines were measured on day 20. CsA treatment significantly reduced LPS-induced systolic blood pressure and the mean 24-h urinary albumin excretion. Pro-inflammatory cytokines IL-6, IL-17, IFN-γ and TNF-α were increased in the LPS treatment group but were reduced in (LPS+CsA) group (P<0.05). Anti-inflammatory cytokine IL-4 was decreased in the LPS group but was increased in (LPS+CsA) group (P<0.05). Cyclosporine A improved preeclampsia signs and attenuated inflammatory responses in the LPS induced preeclampsia rat model which suggests that immunosuppressant might be an alternative management option for preeclampsia.
authors.affiliation: Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou, China; Guangzhou Medical University, Guangzhou, China; The Maternal and Child Health Hospital of HuaDu District, Guangzhou, China.
authors.forename: Bihui
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou, China; Guangzhou Medical University, Guangzhou, China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou, China; Guangzhou Medical University, Guangzhou, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou, China; Guangzhou Medical University, Guangzhou, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department Perinatal Medicine Pregnancy Research Centre and University of Melbourne, Department of Obstetrics and Gynecology, Royal Women's Hospital, Parkville, Victoria, Australia.
authors.forename: Shaun Patrick
authors.initials: SP
authors.lastname: Brennecke
authors.affiliation: Department of Obstetrics, Guangzhou Women and Children's Medical Center, Guangzhou, China; Guangzhou Medical University, Guangzhou, China. Electronic address: huishuliu@hotmail.com.
authors.forename: Huishu
authors.initials: H
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D020567:Fetal Weight / Q000187:drug effects; D005865:Gestational Age; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007249:Inflammation / Q000378:metabolism / Q000517:prevention & control*; D007371:Interferon-gamma / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides; D008297:Male; D011225:Pre-Eclampsia / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D011247:Pregnancy; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D007166:Immunosuppressive Agents; D020381:Interleukin-17; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016572:Cyclosporine
keywords: Cyclosporin A; Cytokines; Immunosuppressive therapy; Preeclampsia