title: A profile of protein-protein interaction: Crystal structure of a lectin-lectin complex.
abstract: Proteins may utilize complex networks of interactions to create/proceed signaling pathways of highly adaptive responses such as programmed cell death. Direct binary interactions study of proteins may help propose models for protein-protein interaction. Towards this goal we applied a combination of thermodynamic kinetics and crystal structure analyses to elucidate the complexity and diversity in such interactions. By determining the heat change on the association of two galactose-specific legume lectins from Butea monosperma (BML) and Spatholobus parviflorus (SPL) belonging to Fabaceae family helped to compute the binding equilibrium. It was extended further by X-ray structural analysis of BML-SPL binary complex. In order to chart the proteins interacting mainly through their interfaces, identification of the nature of forces which stabilized the association of the lectin-lectin complex was examined. Comprehensive analysis of the BMLSPL complex by isothermal titration calorimetry and X-ray crystal structure threw new light on the lectin-lectin interactions suggesting of their use in diverse areas of glycobiology.
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India. Electronic address: suryasukumaran089@gmail.com.
authors.forename: Sukumaran
authors.initials: S
authors.lastname: Surya
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India. Electronic address: abhibio@gmail.com.
authors.forename: Joseph
authors.initials: J
authors.lastname: Abhilash
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India. Electronic address: kgixora@gmail.com.
authors.forename: Krishnan
authors.initials: K
authors.lastname: Geethanandan
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India. Electronic address: csadasivan@rediffmail.com.
authors.forename: Chittalakkottu
authors.initials: C
authors.lastname: Sadasivan
authors.affiliation: Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India. Electronic address: mharidasm@rediffmail.com.
authors.forename: Madhathilkovilakathu
authors.initials: M
authors.lastname: Haridas
mesh_terms: D031290:Butea / Q000737:chemistry; D018360:Crystallography, X-Ray; D007700:Kinetics; D008958:Models, Molecular; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012639:Seeds / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D037121:Plant Lectins
keywords: BML; ITC; SPL