title: Differential toxicity of Al2O3 particles on Gram-positive and Gram-negative sediment bacterial isolates from freshwater.
abstract: The current study was aimed to explore the differential effects on Gram-positive and Gram-negative freshwater sediment bacterial isolates upon exposure to nano-particles and bulk particles of Al2O3 at low concentrations (0.25, 0.5, and 1 mg/L). The Gram-negative Pseudomonas aeruginosa was more susceptible to both the nano-forms and bulk forms than the Gram-positive Bacillus altitudinis. The generation of reactive oxygen species (ROS) and release of lipopolysaccharide due to membrane damage were dependent on the dose of nano-Al2O3. The Fourier transform infrared spectroscopy (FT-IR) studies confirmed the attachment of nano-Al2O3 on bacterial cells, which may lead to subsequent changes in the cell membrane composition and integrity. Internalization of nano-Al2O3 was estimated to be more for P. aeruginosa than for B. altitudinis cells. As a role of defense mechanism, the biofilm formation and production of extracellular polymeric substances (EPSs; polysaccharide and protein) were increased with respect to the concentration of toxicant. Nano-Al2O3 was estimated to cause more DNA damage than the bulk particles in both Gram-positive and Gram-negative bacterial strains.
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632014, India.
authors.forename: M
authors.initials: M
authors.lastname: Bhuvaneshwari
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632014, India.
authors.forename: Sakcham
authors.initials: S
authors.lastname: Bairoliya
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632014, India.
authors.forename: Abhinav
authors.initials: A
authors.lastname: Parashar
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632014, India.
authors.forename: N
authors.initials: N
authors.lastname: Chandrasekaran
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632014, India. amit.mookerjea@gmail.com.
authors.forename: Amitava
authors.identifier: http://orcid.org/0000-0001-8682-4278
authors.initials: A
authors.lastname: Mukherjee
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry / Q000633:toxicity*; D001407:Bacillus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001704:Biopolymers / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects; D004249:DNA Damage; D005618:Fresh Water / Q000382:microbiology; D019015:Geologic Sediments / Q000382:microbiology; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D001704:Biopolymers; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D000537:Aluminum Oxide
keywords: Bacteria; Bulk Al2O3; Cytotoxicity; DNA damage; Nano-Al2O3