title: Evaluation of hyper thermal acid hydrolysis of Kappaphycus alvarezii for enhanced bioethanol production.
abstract: Hyper thermal (HT) acid hydrolysis of Kappaphycus alvarezii, a red seaweed, was optimized to 12% (w/v) seaweed slurry content, 180mM H2SO4 at 140°C for 5min. The maximum monosaccharide concentration of 38.3g/L and 66.7% conversion from total fermentable monosaccharides of 57.6g/L with 120gdw/L K. alvarezii slurry were obtained from HT acid hydrolysis and enzymatic saccharification. HT acid hydrolysis at a severity factor of 0.78 efficiently converted the carbohydrates of seaweed to monosaccharides and produced a low concentration of inhibitory compounds. The levels of ethanol production by separate hydrolysis and fermentation with non-adapted and adapted Kluyveromyces marxianus to high concentration of galactose were 6.1g/L with ethanol yield (YEtOH) of 0.19 at 84h and 16.0g/L with YEtOH of 0.42 at 72h, respectively. Development of the HT acid hydrolysis process and adapted yeast could enhance the overall ethanol fermentation yields of K. alvarezii seaweed.
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, South Korea.
authors.forename: Chae Hun
authors.initials: CH
authors.lastname: Ra
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, South Korea.
authors.forename: Trung Hau
authors.initials: TH
authors.lastname: Nguyen
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, South Korea.
authors.forename: Gwi-Taek
authors.initials: GT
authors.lastname: Jeong
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan 48513, South Korea. Electronic address: skkim@pknu.ac.kr.
authors.forename: Sung-Koo
authors.initials: SK
authors.lastname: Kim
mesh_terms: D056804:Biofuels; D019149:Bioreactors; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005690:Galactose / Q000378:metabolism; D006868:Hydrolysis; D007716:Kluyveromyces / Q000378:metabolism*; D000461:Rhodophyta / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae
chemical_list: D056804:Biofuels; D000431:Ethanol; D005690:Galactose
keywords: Ethanol fermentation; HMF; HT acid hydrolysis; Kappaphycus alvarezii; Kluyveromyces marxianus