title: Oxaliplatin immunohybrid nanoparticles in vitro synergistic suppression evaluation in treatment of colorectal cancer.
abstract: In the present study, we have investigated the enhanced synergistic and apoptotic activity of immunohybrid nanoparticles encapsulating oxaliplatin and covalently conjugated with TRAIL (Apo-2L/CD-253). Time-dependent cytotoxicity activity of nanoparticles was determined by MTT assay in HT-29 cells. Nuclear morphological changes and assessment of apoptotic ratio was analyzed by DAPI (4'6-diamidino-2-phenylindole) staining and annexin-propidium iodide (PI) assay. Cell-cycle analysis of oxaliplatin in HT-29 cell was analyzed by flow cytometry at 72 h. Furthermore, molecular mechanisms related to oxaliplatin-induced anticancer activity was explored by western blot analysis. Our study revealed appreciable time-dependent cytotoxicity, apoptotic, and synergistic activity of oxaliplatin immunohybrid nanoparticles.
authors.affiliation: a Department of Pharmaceutics , J. S. S. College of Pharmacy (off-Campus), J. S. S University , Mysore , Karnataka , India.
authors.forename: Shashank
authors.initials: S
authors.lastname: Tummala
authors.affiliation: a Department of Pharmaceutics , J. S. S. College of Pharmacy (off-Campus), J. S. S University , Mysore , Karnataka , India.
authors.forename: K
authors.initials: K
authors.lastname: Gowthamarajan
authors.affiliation: b Department of Pharmacology , J. S. S. College of Pharmacy (off-Campus), J. S. S University , Mysore , Karnataka , India.
authors.forename: M N
authors.initials: MN
authors.lastname: Satish Kumar
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016503:Drug Delivery Systems / Q000379:methods*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology; D000077150:Oxaliplatin; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000737:chemistry / Q000494:pharmacology
chemical_list: D009944:Organoplatinum Compounds; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D000077150:Oxaliplatin
keywords: Immunonanoparticles; TRAIL; oxaliplatin; synergestic