title: Functional analysis of the uL11 protein impact on translational machinery.
abstract: The ribosomal GTPase associated center constitutes the ribosomal area, which is the landing platform for translational GTPases and stimulates their hydrolytic activity. The ribosomal stalk represents a landmark structure in this center, and in eukaryotes is composed of uL11, uL10 and P1/P2 proteins. The modus operandi of the uL11 protein has not been exhaustively studied in vivo neither in prokaryotic nor in eukaryotic cells. Using a yeast model, we have brought functional insight into the translational apparatus deprived of uL11, filling the gap between structural and biochemical studies. We show that the uL11 is an important element in various aspects of 'ribosomal life'. uL11 is involved in 'birth' (biogenesis and initiation), by taking part in Tif6 release and contributing to ribosomal subunit-joining at the initiation step of translation. uL11 is particularly engaged in the 'active life' of the ribosome, in elongation, being responsible for the interplay with eEF1A and fidelity of translation and contributing to a lesser extent to eEF2-dependent translocation. Our results define the uL11 protein as a critical GAC element universally involved in trGTPase 'productive state' stabilization, being primarily a part of the ribosomal element allosterically contributing to the fidelity of the decoding event.
authors.affiliation: a Department of Molecular Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Wawiórka
authors.affiliation: a Department of Molecular Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Eliza
authors.initials: E
authors.lastname: Molestak
authors.affiliation: a Department of Molecular Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Szajwaj
authors.affiliation: a Department of Molecular Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Michalec-Wawiórka
authors.affiliation: a Department of Molecular Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Boguszewska
authors.affiliation: a Department of Molecular Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Borkiewicz
authors.affiliation: b Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw , Warsaw , Poland.
authors.forename: Vladyslava
authors.initials: V
authors.lastname: Liudkovska
authors.affiliation: b Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw , Warsaw , Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kufel
authors.affiliation: a Department of Molecular Biology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Tchórzewski
mesh_terms: D009154:Mutation / Q000235:genetics; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D021122:Protein Subunits / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D012269:Ribosomal Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D012269:Ribosomal Proteins; C525863:Rpl12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: L12/uL11; biogenesis; ribosomal protein; ribosomal stalk; ribosome; translation; translational fidelity