title: [Prokaryotic expression of Staphylococcus aureus Clumping factor B and evaluation of the antiserum-mediated opsonic activity].
abstract: Staphylococcus aureus is a major cause of hospital-acquired infection. Because the bacteria are very easy to become resistant to antibiotics, vaccination is a main method against S. aureus infection. Clumping factor B (ClfB) is an adhesion molecule essential for S. aureus to colonize in the host mucosa and is regarded as an important target antigen. In this study, we successfully used Escherichia coli to express a segment encoding the N1-N3 regions of ClfB protein (Truncated-ClfB) cloned from S. aureus. The protein was purified by affinity and ion exchange chromatographies and gel filtration. Rabbits were immunized three times with purified Truncated-ClfB. After that, blood was collected to prepare serum which were then used for measurement of antibody level. Phagocytosis of S. aureus opsonized by the serum was determined by a flow cytometry. Results show that the serum IgG titer reached 1:640 000. Phagocytosed S. aureus by polymorphonuclear leukocytes were significantly more when the bacteria were opsonized by the serum from Truncated-ClfB immunized rabbits than those from no immunized group (P < 0.01). Therefore, the results indicated that Truncated-ClfB could be a promising vaccine candidate against S. aureus infection.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Shanshan
authors.initials: S
authors.lastname: Cao
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chunling
authors.initials: C
authors.lastname: Xuan
authors.forename: Youqiang
authors.initials: Y
authors.lastname: Wu
authors.forename: Songhua
authors.initials: S
authors.lastname: Hu
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004926:Escherichia coli; D005434:Flow Cytometry; D007106:Immune Sera; D007074:Immunoglobulin G / Q000097:blood; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis; D011817:Rabbits; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G; D009895:Opsonin Proteins; C434058:clfB protein, Staphylococcus aureus