title: Probing the interaction of a new synthesized CdTe quantum dots with human serum albumin and bovine serum albumin by spectroscopic methods.
abstract: A novel CdTe quantum dots (QDs) were prepared in aqueous phase via a facile method. At first, poly (acrylic amide) grafted onto sodium alginate (PAAm-g-SA) were successfully synthesized and then TGA capped CdTe QDs (CdTe-TGA QDs) were embed into it. The prepared CdTe-PAAm-g-SA QDs were optimized and characterized by transmission electron microscopy (TEM), thermo-gravimetric (TG) analysis, Fourier transform infrared (FT-IR), UV-vis and fluorescence spectroscopy. The characterization results indicated that CdTe-TGA QDs, with particles size of 2.90 nm, were uniformly dispersed on the chains of PAAm-g-SA biopolymer. CdTe-PAAm-g-SA QDs also exhibited excellent UV-vis absorption and high fluorescence intensity. To explore biological behavior of CdTe-PAAm-g-SA QDs, the interactions between CdTe-PAAm-g-SA QDs and human serum albumin (HSA) (or bovine serum albumin (BSA)) were investigated by cyclic voltammetry, FT-IR, UV-vis, and fluorescence spectroscopic. The results confirmed the formation of CdTe-PAAm-g-SA QDs-HSA (or BSA) complex with high binding affinities. The thermodynamic parameters (ΔG<0, ΔH<0 and ΔS<0) were indicated that binding reaction was spontaneous and van der Waals interactions and hydrogen-bond interactions played a major role in stabilizing the CdTe-PAAm-g-SA QDs-HSA (or BSA) complexes. The binding distance between CdTe-PAAm-g-SA QDs and HSA (or BSA)) was calculated about 1.37 nm and 1.27 nm, respectively, according to Forster non-radiative energy transfer theory (FRET). Analyzing FT-IR spectra showed that the formation of QDs-HSA and QDs-BSA complexes led to conformational changes of the HSA and BSA proteins. All these experimental results clarified the effective transportation and elimination of CdTe-PAAm-g-SA QDs in the body by binding to HSA and BSA, which could be a useful guideline for the estimation of QDs as a drug carrier.
authors.affiliation: Department of Chemistry, Payame Noor University, PO BOX 19395-3697, Tehran, Iran. Electronic address: rezanejad@pnu.ac.ir.
authors.forename: Ghasem Rezanejade
authors.initials: GR
authors.lastname: Bardajee
authors.affiliation: Department of Chemistry, Payame Noor University, PO BOX 19395-3697, Tehran, Iran.
authors.forename: Zari
authors.initials: Z
authors.lastname: Hooshyar
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000464:Alginates / Q000737:chemistry; D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry; D002417:Cattle; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006860:Hydrogen Bonding; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D011485:Protein Binding; D045663:Quantum Dots / Q000737:chemistry* / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013691:Tellurium / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D013818:Thermogravimetry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000464:Alginates; D019187:Cadmium Compounds; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013691:Tellurium; D000099166:Polyacrylamides; C028337:cadmium telluride
keywords: Fluorescence quenching; QDs; Serum albumin; Sodium alginate