title: Protein Quality Assessment on Saliva Samples for Biobanking Purposes.
abstract: Biobank saliva sample quality depends on specific criteria applied to collection, processing, and storage. In spite of the growing interest in saliva as a diagnostic fluid, few biobanks currently store large collections of such samples. The development of a standard operating procedure (SOP) for saliva collection and quality control is fundamental for the establishment of a new saliva biobank, which stores samples to be made available to the saliva research community. Different collection methods were tested regarding total volume of protein obtained, protein content, and protein profiles, and the results were used to choose the best method for protein studies. Furthermore, the impact of the circadian variability and inter- and intraindividual differences, as well as the saliva sample stability at room temperature, were also evaluated. Considering our results, a sublingual cotton roll method for saliva collection proved to produce saliva with the best characteristics and should be applied in the morning, whenever possible. In addition, there is more variability in salivary proteins between individuals than in the same individual for a 5-month period. According to the electrophoretic protein profile, protein stability is guaranteed for 24 hours at room temperature and the protein degradation profile and protein identification were characterized. All this information was used to establish an SOP for saliva collection, processing, and storage in a biobank. We conclude that it is possible to collect saliva using an easy and inexpensive protocol, resulting in saliva samples for protein analysis with sufficient quality for biobanking purposes.
authors.affiliation: 1 Institute of Health Sciences (ICS), Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa , Viseu, Portugal .
authors.forename: Nuno
authors.initials: N
authors.lastname: Rosa
authors.affiliation: 2 Department of Biology, University of Aveiro , Aveiro, Portugal .
authors.forename: Jéssica
authors.initials: J
authors.lastname: Marques
authors.affiliation: 1 Institute of Health Sciences (ICS), Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa , Viseu, Portugal .
authors.forename: Eduardo
authors.initials: E
authors.lastname: Esteves
authors.affiliation: 1 Institute of Health Sciences (ICS), Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa , Viseu, Portugal .
authors.forename: Mónica
authors.initials: M
authors.lastname: Fernandes
authors.affiliation: 3 CNC-Center for Neuroscience and Cell Biology, University of Coimbra , Coimbra, Portugal .|4 Biocant-Biotechnology Innovation Center , Cantanhede, Portugal .
authors.forename: Vera M
authors.initials: VM
authors.lastname: Mendes
authors.affiliation: 5 Biobanco-IMM, Instituto de Medicina Molecular-Faculdade de Medicina de Lisboa , Lisbon, Portugal .
authors.forename: Ângela
authors.initials: Â
authors.lastname: Afonso
authors.affiliation: 5 Biobanco-IMM, Instituto de Medicina Molecular-Faculdade de Medicina de Lisboa , Lisbon, Portugal .
authors.forename: Sérgio
authors.initials: S
authors.lastname: Dias
authors.affiliation: 5 Biobanco-IMM, Instituto de Medicina Molecular-Faculdade de Medicina de Lisboa , Lisbon, Portugal .
authors.forename: Joaquim Polido
authors.initials: JP
authors.lastname: Pereira
authors.affiliation: 3 CNC-Center for Neuroscience and Cell Biology, University of Coimbra , Coimbra, Portugal .|4 Biocant-Biotechnology Innovation Center , Cantanhede, Portugal .
authors.forename: Bruno
authors.initials: B
authors.lastname: Manadas
authors.affiliation: 1 Institute of Health Sciences (ICS), Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa , Viseu, Portugal .
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Correia
authors.affiliation: 1 Institute of Health Sciences (ICS), Center for Interdisciplinary Research in Health (CIIS), Universidade Católica Portuguesa , Viseu, Portugal .
authors.forename: Marlene
authors.initials: M
authors.lastname: Barros
mesh_terms: D000328:Adult; D018070:Biological Specimen Banks; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D008297:Male; D055550:Protein Stability; D011786:Quality Control; D012463:Saliva / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods / Q000592:standards*; D013696:Temperature; D055815:Young Adult
chemical_list: D012471:Salivary Proteins and Peptides