title: Biodegradation of marine crude oil pollution using a salt-tolerant bacterial consortium isolated from Bohai Bay, China.
abstract: This study aims at constructing an efficient bacterial consortium to biodegrade crude oil spilled in China's Bohai Sea. In this study, TCOB-1 (Ochrobactrum), TCOB-2 (Brevundimonas), TCOB-3 (Brevundimonas), TCOB-4 (Bacillus) and TCOB-5 (Castellaniella) were isolated from Bohai Bay. Through the analysis of hydrocarbon biodegradation, TCOB-4 was found to biodegrade more middle-chain n-alkanes (from C17 to C23) and long-chain n-alkanes (C31-C36). TCOB-5 capable to degrade more n-alkanes including C24-C30 and aromatics. On the basis of complementary advantages, TCOB-4 and TCOB-5 were chosen to construct a consortium which was capable of degrading about 51.87% of crude oil (2% w/v) after 1week of incubation in saline MSM (3% NaCl). It is more efficient compared with single strain. In order to biodegrade crude oil, the construction of bacterial consortia is essential and the principle of complementary advantages could reduce competition between microbes.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Xinfei
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, People's Republic of China. Electronic address: zhaolin@tju.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Adam
mesh_terms: D000473:Alkanes / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism; D061006:Bays / Q000737:chemistry* / Q000382:microbiology; D001673:Biodegradation, Environmental; D002681:China; D006838:Hydrocarbons; D059013:Microbial Consortia; D042041:Ochrobactrum / Q000378:metabolism; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D059392:Petroleum Pollution; D055049:Salt Tolerance
chemical_list: D000473:Alkanes; D006838:Hydrocarbons; D010578:Petroleum
keywords: Bacterial consortium; Biodegradation; Complementary advantages; Crude oil; Marine