title: Expression of CD27, CD28 and IL-17A in peripheral blood from patients with colorectal carcinoma.
abstract: OBJECTIVE
  To compare the different expressions of CD27, CD28, IL-17A, IFN-γ and TNF-α in the peripheral blood sampled from patients with colorectal carcinoma and healthy volunteers.
  
  
  METHODS
  Vδ2 T cells were isolated from the peripheral blood mononuclear cells (PBMCs) of patients with the colorectal carcinoma (CRC, n = 30) and healthy controls (HC, n = 21). The proportion of CD27, CD28, IL-17A, IFN-γ and TNF-α of Vδ2 T cells was detected by the flow cytometry.
  
  
  RESULTS
  We found that the proportion of IL-17A of Vδ2 T cells in PBMCs was higher in the CRC vs. the HC group (p < 0.05). A significant positive correlation was observed between the expression of IFN-γ and TNF-α of Vδ2 T cells. In the CRC patients, the proportions of IL-17A of CD27- Vδ2 T cells and CD28+ Vδ2 T cells were higher than those of CD27+ Vδ2 T cells and CD28- Vδ2 T cells, whereas the expression of IFN-γ and TNF-α of CD27-Vδ2 T cells was lower than that of CD27+ Vδ2 T cells.
  
  
  CONCLUSIONS
  Vδ2 T cells from PBMCs had higher expression of IL-17A in CRC patients than that in the HC group. The expression of IFN-γ and TNF-α of Vδ2 T cells from PBMCs was positively correlated. The cytokine profiles of peripheral Vδ2 T cells were likely determined by a CD27 and CD28 involving mechanism.
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College, Jiangsu, China. xyfyfeisj99@163.com.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Han
authors.forename: F-X
authors.initials: FX
authors.lastname: Chen
authors.forename: X-T
authors.initials: XT
authors.lastname: Lu
authors.forename: J-Q
authors.initials: JQ
authors.lastname: Liu
authors.forename: S-J
authors.initials: SJ
authors.lastname: Fei
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D018106:CD28 Antigens / Q000097:blood* / Q000235:genetics; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020381:Interleukin-17 / Q000097:blood* / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000378:metabolism*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000097:blood* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D018106:CD28 Antigens; D016207:Cytokines; C508717:IL17A protein, human; D020381:Interleukin-17; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7