title: [Extraction of miRNA from Glycyrrhiza uralensis Decoction and Its Effect on Immune Cells].
abstract: OBJECTIVE
  To extract microRNA(miRNA) from Glycyrrhiza uralensis(liquorice) decoction and to explore its effect on mmune cells.
  
  
  METHODS
  With dried processed liquorice, the water decoction was prepared according to the conventional method and subsequently concentrated by rotary evaporation. The concentrated decoction was further freeze-dried by freeze dryer, and miRNAs were extracted with Plant MicroRNA Extraction Kit. The extracted miRNA was digested by DNase I and then analyzed through the agarose gell electrophoresis. The PBMC was isolated from healthy volunteers and treated respectively by liquorice water extract, glycyrrhizic acid, glycyrrhetic acid and liquorice miRNAs. After 24 hours, the cells numbers were counted, and the changes of cell morphology were observed. The expression of CD3, CD56 and HLA-DR were analyzed by flow cytometry to identify the change of cell subsets in PBMC.
  
  
  RESULTS
  miRNAs could be extracted from the decoction of dried liquorice which further confirmed the stability of miRNAs. The in vitro culture experiment showed that,compared with the controls, PBMC treated with liquorice miRNAs appeared apparent cell aggregation and increased cell number and HLA-DR+ cells proportion.
  
  
  CONCLUSIONS
  The miRNAs are successfully extracted from the freeze-dried decoction of dried liquorice. It is indicated that liquorice miRNAs have significant stimulative effects on the growth of PBMC and HLA-DR+ cells subset.
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Shao
authors.forename: Mian
authors.initials: M
authors.lastname: He
authors.forename: Jia-si
authors.initials: JS
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Xiang
authors.forename: Shu-lin
authors.initials: SL
authors.lastname: Huang
mesh_terms: D029882:Glycyrrhiza uralensis / Q000737:chemistry / Q000235:genetics*; D019695:Glycyrrhizic Acid / Q000737:chemistry*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D035683:MicroRNAs / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D035683:MicroRNAs; D010936:Plant Extracts; D019695:Glycyrrhizic Acid