title: The antitumor effect of TIG3 in liver cancer cells is involved in ERK1/2 inhibition.
abstract: Tazarotene-induced gene 3 (TIG3) was first characterized in tazarotene-treated human keratinocytes and identified as a retinoic acid responder gene, an important mediator of antitumor effects by retinoids. In this study, we aim to investigate the inhibitory effect of TIG3 on the growth of liver cancer and explore its underlying mechanism. Human hepatocellular carcinoma (HCC) Hep3B cells were transfected with plasmid GV141 carrying full-length TIG3 complementary DNA (cDNA). The effects of TIG3 on cell proliferation, apoptosis, and migration were determined in vitro. The suppressor effect of TIG3 on tumor growth was evaluated in vivo in a nude mouse HCC model. We observed that TIG3 expression is decreased in the Hep3B cell line as well as primary HCC tumors, and TIG3 expression inversely correlates with Ki-67 expression. Overexpression of TIG3 suppresses tumor growth in HCC both in vitro and in vivo via ERK1/2 inhibition by promoting apoptosis and inhibiting proliferation and migration. These findings identify TIG3 as an attractive therapeutic target for HCC.
authors.affiliation: Department of Gastroenterology and Hepatology, First Municipal's People Hospital of Guangzhou, Guangzhou Medical University, 1 Panfu Road, Guangzhou, 510180, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Guangzhou Medical University, No. 250 Changgang Dong Road, Guangzhou, 510260, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Pathology, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Degui
authors.initials: D
authors.lastname: Liao
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Guangzhou Medical University, No. 250 Changgang Dong Road, Guangzhou, 510260, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, Guangzhou, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Guangzhou Institute of Cardiovascular Disease, Guangzhou, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Guangzhou Medical University, No. 250 Changgang Dong Road, Guangzhou, 510260, China. yanghui030454@gmail.com.
authors.forename: Hui
authors.identifier: http://orcid.org/0000-0002-9546-7484
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology and Hepatology, First Municipal's People Hospital of Guangzhou, Guangzhou Medical University, 1 Panfu Road, Guangzhou, 510180, China. nieyq@medmail.com.cn.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Nie
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D064593:Heterografts; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018613:Microscopy, Confocal; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D018168:Receptors, Retinoic Acid / Q000235:genetics*; D014162:Transfection
chemical_list: C437968:PLAAT4 protein, human; D018168:Receptors, Retinoic Acid
keywords: Antitumor effect; ERK; Hepatocellular carcinoma; TIG3