title: Mechanistic contribution of electroconductive hydroxyapatite-titanium disilicide composite on the alignment and proliferation of cells.
abstract: We elucidate here the mechanistic contribution of a novel electroconductive hydroxyapatite-20 wt.% titanium disilicide (HA-TiSi2) composite system in favorably modulating osteoblast functions in relation to the monolithic HA. The higher electrical conductivity of HA-TiSi2(σDC ∼ 67.117 ± 3.57 S/m) in comparison to glass sample effectively guided the electroactive myoblast, leading to their significant alignment and proliferation. This favorable behavior is attributed to the formation of small electrochemical cells between HA and TiSi2phase, which produce a small electric field, directing the electroactive myoblast to migrate and grow in a particular direction. In contrast, no impact of TiSi2on osteoblast function was observed because of their inability to respond to small electric field. However, thein vitrobioactivity in simulated body fluid indicated the nucleation and growth of apatite crystals. Moreover, in the context of load-bearing capability, the presence of 20 wt.% TiSi2in HA led to increase in the fracture toughness by ∼100%. This study underscores the effectiveness of HA-TiSi2in favorably modulating the cellular activity, myoblast in particular.
authors.affiliation: Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, El Paso, TX, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, El Paso, TX, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Nune
authors.affiliation: Materials Research Centre, Indian Institute of Science, Bangalore, Karnataka, India.
authors.forename: B
authors.initials: B
authors.lastname: Basu
authors.affiliation: Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, El Paso, TX, USA dmisra2@utep.edu.
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
mesh_terms: D000818:Animals; D001031:Apatites / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D017886:Durapatite / Q000737:chemistry*; D004553:Electric Conductivity; D008422:Materials Testing; D051379:Mice; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D017640:Silicates / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering; D014025:Titanium / Q000737:chemistry*; D016474:Weight-Bearing
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D017640:Silicates; C103828:titanium silicide; D017886:Durapatite; D014025:Titanium
keywords: Electroconductive; bone tissue engineering; electrical field; hydroxyapatite; titanium disilicide