title: Quantitative assessment of ribosome drop-off in E. coli.
abstract: Premature ribosome drop-off is one of the major errors in translation of mRNA by ribosomes. However, repeated analyses of Ribo-seq data failed to quantify its strength inE. coli Relying on a novel highly sensitive data analysis method we show that a significant rate of ribosome drop-off is measurable and can be quantified also when cells are cultured under non-stressing conditions. Moreover, we find that the drop-off rate is highly variable, depending on multiple factors. In particular, under environmental stress such as amino acid starvation or ethanol intoxication, the drop-off rate markedly increases.
authors.affiliation: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany.
authors.forename: Celine
authors.initials: C
authors.lastname: Sin
authors.affiliation: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany.
authors.forename: Davide
authors.initials: D
authors.lastname: Chiarugi
authors.affiliation: Department of Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14476 Potsdam, Germany angelo.valleriani@mpikg.mpg.de.
authors.forename: Angelo
authors.initials: A
authors.lastname: Valleriani
mesh_terms: D000596:Amino Acids / Q000172:deficiency; D018389:Codon, Nonsense / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity; D015233:Models, Statistical; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D000596:Amino Acids; D018389:Codon, Nonsense; D000431:Ethanol