title: Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis.
abstract: Aberrant expression of the miR-129 family has been found in several types of cancer, yet its expression and potential biologic role in breast cancer remain largely unknown. In the present study, we found that miR-129-2 was consistently downregulated in the breast cancer specimens and cell lines. Overexpression of miR-129-2-3p markedly suppressed breast cancer cell proliferation and induced its apoptosis. In addition, a luciferase reporter assay revealed that miR-129-2-3p suppressed BCL2L2 expression. Furthermore, BCL2L2 was able to reverse miR-129-2-3p-mediated cell apoptosis, indicating that BCL2L2 plays a crucial role in mediating the tumor-suppressive role of miR-129-2-3p. Moreover, bisulfite DNA sequencing PCR (BSP) analysis identified that promoter hypermethylation was responsible for the downregulation of miR-129-2 in breast cancer. Collectively, our findings indicate that miR-129-2 is downregulated in breast cancer cells by promoter hypermethylation. Moreover, downregulation of miR-129-2 results in BCL2L2 overexpression and disease progression in breast cancer patients.
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Gastroenterology, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Oncology, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College, Guangzhou, Guangdong 510180, P.R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Radiation Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Qin
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Sang
authors.affiliation: Department of Medicine, Gaoming Heshui Hospital, Foshan, Guangdong 528500, P.R. China.
authors.forename: Liang-Ming
authors.initials: LM
authors.lastname: Deng
authors.affiliation: Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Lv
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D049109:Cell Proliferation; D019175:DNA Methylation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference
chemical_list: D051017:Apoptosis Regulatory Proteins; C491073:BCL2L2 protein, human; D035683:MicroRNAs; C541915:Mirn129 microRNA, human