title: Photo Control of Protein Function Using Photoactive Yellow Protein.
abstract: Photoswitchable proteins are becoming increasingly common tools for manipulating cellular processes with high spatial and temporal precision. Photoactive yellow protein (PYP) is a small, water-soluble protein that undergoes a blue light induced change in conformation. It can serve as a scaffold for designing new tools to manipulate biological processes, but with respect to other protein scaffolds it presents some technical challenges. Here, we present practical information on how to overcome these, including how to synthesize the PYP chromophore, how to express and purify PYP, and how to screen for desired activity.
authors.affiliation: Department of Chemistry, University of Toronto, 80 St. George St., Toronto, ON, Canada, M5S 3H6.
authors.forename: Jakeb M
authors.initials: JM
authors.lastname: Reis
authors.affiliation: Department of Chemistry, University of Toronto, 80 St. George St., Toronto, ON, Canada, M5S 3H6. awoolley@chem.utoronto.ca.
authors.forename: G Andrew
authors.initials: GA
authors.lastname: Woolley
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002850:Chromatography, Gel / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D003373:Coumaric Acids / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D008027:Light; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D020283:Photoreceptors, Microbial / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D003373:Coumaric Acids; D020283:Photoreceptors, Microbial; D011993:Recombinant Fusion Proteins; C050162:photoactive yellow protein, Bacteria
keywords: Optogenetic; PYP; Photoactive yellow protein; Protein design; Protein engineering