title: Direct screening of tobacco indicators in urine and saliva by Atmospheric Pressure Solid Analysis Probe coupled to quadrupole-time of flight mass spectrometry (ASAP-MS-Q-TOF-).
abstract: A new screening method has been explored for direct analysis of tobacco smoke biomarkers in biological matrices (i.e., saliva and urine). Single run analysis using Atmospheric pressure Solid Analysis Probe (ASAP) and high resolution mass spectrometry with quadrupole and time of flight detector has been applied directly to some biological samples (i.e., urine and saliva), providing a fast, efficient and sensitive method of identification. The method has been applied to saliva and urine samples from heavy tobacco smokers for exposure studies. Nicotine itself, nicotine metabolites (i.e., cotinine, trans-3'-hydroxycotinine, nicotine-N-glucuronide) and other related tobacco smoke toxic compounds (i.e., NNK 4-[methyl(nitroso)amino]-1-(3-pyridinyl)-1-butanone, anatabine) were found in the analyzed samples. The identification of compounds was confirmed by ultrahigh performance liquid chromatography with MS-triple quadrupole detector after sample treatment. Different temporal trends and biomarkers behavior have been found in time series related samples. Both methods are compared for screening of these biological matrices.
authors.affiliation: Aragon Institute of Engineering Research (I3A), EINA, Department of Analytical Chemistry, University of Zaragoza, María de Luna 3, 50018 Zaragoza, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Carrizo
authors.affiliation: Smoking Cessation Unit, Department of Medicine, Psychiatry and Dermatology, Faculty of Medicine, University of Zaragoza, Domingo Miral s/n, 50009 Zaragoza, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Nerín
authors.affiliation: Aragon Institute of Engineering Research (I3A), EINA, Department of Analytical Chemistry, University of Zaragoza, María de Luna 3, 50018 Zaragoza, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Domeño
authors.affiliation: Aragon Institute of Engineering Research (I3A), EINA, Department of Analytical Chemistry, University of Zaragoza, María de Luna 3, 50018 Zaragoza, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Alfaro
authors.affiliation: Aragon Institute of Engineering Research (I3A), EINA, Department of Analytical Chemistry, University of Zaragoza, María de Luna 3, 50018 Zaragoza, Spain. Electronic address: cnerin@unizar.es.
authors.forename: Cristina
authors.initials: C
authors.lastname: Nerín
mesh_terms: D001274:Atmospheric Pressure; D015415:Biomarkers / Q000032:analysis / Q000097:blood* / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012463:Saliva / Q000737:chemistry
chemical_list: D015415:Biomarkers
keywords: ASAP; Biomarkers; Cancer; Direct analysis; Screening; Tobacco exposure