title: Transcriptome-Wide Identification and Expression Profiling of the DOF Transcription Factor Gene Family in Chrysanthemum morifolium.
abstract: The family of DNA binding with one finger (DOF) transcription factors is plant specific, and these proteins contain a highly conserved domain (DOF domain) of 50-52 amino acids that includes a C2C2-type zinc finger motif at the N-terminus that is known to function in a number of plant processes. Here, we characterized 20 DOF genes in the important ornamental species chrysanthemum (Chrysanthemum morifolium) based on transcriptomic sequences. Phylogenetic analysis identified one pair of putative orthologous proteins in Arabidopsis and chrysanthemum and six pairs of paralogous proteins in chrysanthemum. Conserved motifs in the DOF proteins shared by Arabidopsis and chrysanthemum were analyzed using MEME. Bioinformatics analysis revealed that 13 CmDOFs could be targeted by 16 miRNA families. Moreover, we used 5' RLM-RACE to map the cleavage sites in CmDOF3, 15, and 21. The expression of these 20 genes in response to phytohormone treatments and abiotic stresses was characterized, and the expression patterns of six pairs of paralogous CmDOF genes were found to completely differ from one another, except for CmDOF6 and CmDOF7. This work will promote our research of the various functions of DOF gene family members in plant hormone and stress responses.
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Gao
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Peiling
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xin
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Fan
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Kunkun
authors.initials: K
authors.lastname: Zhao
authors.affiliation: College of Horticulture, Nanjing Agricultural University Nanjing, China.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
keywords: Chrysanthemum morifolium; DNA binding with one finger; phylogenetic analysis; stress response; transcription factors