title: Quantifying Current Events Identifies a Novel Endurance Regulator.
abstract: In nongrowing microbes, proteome turnover is reduced and identification of newly synthesized, low-abundance proteins is challenging. Babin and colleagues recently utilized bio-orthogonal noncanonical amino acid tagging (BONCAT) to identify actively synthesized proteins in nongrowing Pseudomonas aeruginosa, discovering a regulator whose influences range from biofilm formation to secondary metabolism.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA; Rutgers Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
authors.forename: Theresa C
authors.initials: TC
authors.lastname: Henry
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA; Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA. Electronic address: mbrynild@princeton.edu.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Brynildsen
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D008828:Microbiological Techniques / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism* / Q000502:physiology
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D020543:Proteome; C506098:azidohomoalanine; D000409:Alanine
keywords: BONCAT; Pseudomonas aeruginosa; SutA; nongrowing cells; transcription