title: Mitochondria-targeted ruthenium (II) polypyridyl complexes with benzofuran group for live cell imaging.
abstract: Labeling and imaging mitochondria have attracted considerable interest because of its involvement in early stage apoptosis and necrotic cell death. Various highly specific and photostable fluorescent probes for mitochondria are in demand. In the present study, two novel Ru(II) polypyridine complexes Ru1 and Ru2 were developed to act as mitochondrial fluorescence probes. In comparison with the commercially available mitochondrial trackers, Ru1 possesses high mitochondria-specificity, superior photostability, high resistance to the loss of mitochondrial membrane potential and appreciable tolerance to environmental change, allowing imaging of the mitochondrial morphological changes over long periods of time. Combined results indicate that Ru1 may contribute to the future development of staining agents for organelle-selective imaging in living cells.
authors.affiliation: School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, P.R. China. Electronic address: xuli473@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, P.R. China.
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, P.R. China.
authors.forename: Liu-Ming
authors.initials: LM
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, P.R. China.
authors.forename: Ye-Yi
authors.initials: YY
authors.lastname: Xie
authors.affiliation: School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, P.R. China.
authors.forename: Jie-Xing
authors.initials: JX
authors.lastname: Liang
authors.affiliation: MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China. Electronic address: ceschh@mail.sysu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Chao
mesh_terms: D001572:Benzofurans / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D012428:Ruthenium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001572:Benzofurans; D005456:Fluorescent Dyes; D012428:Ruthenium; C105430:benzofuran
keywords: Imaging; Mitochondria; Photostability; Probe; Ru(II) complex