title: Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse.
abstract: The adaptive response to extreme endurance exercise might involve transcriptional and translational regulation by microRNAs (miRNAs). Therefore, the objective of the present study was to perform an integrated analysis of the blood transcriptome and miRNome (using microarrays) in the horse before and after a 160 km endurance competition. A total of 2,453 differentially expressed genes and 167 differentially expressed microRNAs were identified when comparing pre- and post-ride samples. We used a hypergeometric test and its generalization to gain a better understanding of the biological functions regulated by the differentially expressed microRNA. In particular, 44 differentially expressed microRNAs putatively regulated a total of 351 depleted differentially expressed genes involved variously in glucose metabolism, fatty acid oxidation, mitochondrion biogenesis, and immune response pathways. In an independent validation set of animals, graphical Gaussian models confirmed that miR-21-5p, miR-181b-5p and miR-505-5p are candidate regulatory molecules for the adaptation to endurance exercise in the horse. To the best of our knowledge, the present study is the first to provide a comprehensive, integrated overview of the microRNA-mRNA co-regulation networks that may have a key role in controlling post-transcriptomic regulation during endurance exercise.
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Núria
authors.initials: N
authors.lastname: Mach
authors.affiliation: INRA, MaIAGE, Jouy-en-Josas, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Plancade
authors.affiliation: Swiss Institute of Equine Medicine, Institute of Genetics, University of Bern and Agroscope, Bern, Switzerland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Pacholewska
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Lecardonnel
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Rivière
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Marco
authors.initials: M
authors.lastname: Moroldo
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Vaiman
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Morgenthaler
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Beinat
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
authors.forename: Alizée
authors.initials: A
authors.lastname: Nevot
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.|Université Paris-Est, Ecole Vétérinaire d'Alfort, Maisons-Alfort, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Robert
authors.affiliation: Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.|Integrative Biology and Exercice Adaptation unit (UBIAE), EA7362, Université d'Evry Val d'Essonne, Evry, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Barrey
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005786:Gene Expression Regulation; D006736:Horses / Q000235:genetics / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D010807:Physical Endurance / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger