title: Molecular mechanism of endocrine system impairment by 17α-methyltestosterone in gynogenic Pengze crucian carp offspring.
abstract: The effects of synthetic androgen 17α-methyltestosterone (MT) on endocrine impairment were examined in crucian carp. Immature 7-month old mono-female Pengze crucian carp (Pcc) F2 offspring were exposed to 50 and 100 μg/L of MT (week 2, 4, and 8). Gonadosomatic index, hepatosomatic index and intestine weight altered considerably and oocyte development was repressed. In the treatment groups, ovarian 11-ketotestosterone decreased, whereas 17β-estradiol and testosterone increased, and ovarian aromatase activities increased at week 4. However, in the brain tissue, those values significantly decreased. Quantitative RT-PCR analysis demonstrated changes in steroid receptor genes and upregulation of steroidogenic genes (Pcc-3bhsd, Pcc-11bhsd2 Pcc-cyp11a1), while the other three steroidogenic genes (Pcc-cyp17a1, Pcc-cyp19a1a and Pcc-star) decreased from week 4 to week 8. Ovarian, hepatic Pcc-vtg B and vitellogenin concentration increased in both 50 and 100 μg/L of MT exposure groups. This study adds further information regarding the effects of androgens on the development of previtellogenic oocytes, which suggests that MT could directly target estrogen signaling pathway, or indirectly affect steroidogenesis and vitellogenesis.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China; Freshwater Fisheries Research Center, Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, China; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, HZAU, Wuhan 430070, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Freshwater Fisheries Research Center, Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, China; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
authors.forename: Jiazhang
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Jiancao
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China; Freshwater Fisheries Research Center, Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, China; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Freshwater Fisheries Research Center, Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, China; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
authors.forename: Xuwen
authors.initials: X
authors.lastname: Bing
authors.affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, HZAU, Wuhan 430070, China.
authors.forename: Zexia
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: zzwang@nwsuaf.edu.cn.
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000728:Androgens / Q000633:toxicity*; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002347:Carps / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D004703:Endocrine System / Q000187:drug effects* / Q000378:metabolism; D004958:Estradiol / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008777:Methyltestosterone / Q000633:toxicity*; D010063:Ovum / Q000187:drug effects / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D012727:Sex Characteristics; D013739:Testosterone / Q000235:genetics / Q000378:metabolism; D014818:Vitellogenesis / Q000187:drug effects* / Q000235:genetics; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism
chemical_list: D000728:Androgens; D052244:Endocrine Disruptors; D011987:Receptors, Steroid; D014819:Vitellogenins; D013739:Testosterone; D004958:Estradiol; D001141:Aromatase; D008777:Methyltestosterone
keywords: 17α-methyltestosterone; Estrogen signalling; Gynogenic Pengze crucian carp; Steroid hormones; Steroidogenesis; Vitellogenesis