title: [Overexpression of FKS1 to improve yeast autolysis-stress].
abstract: With the development of high gravity brewing, yeast cells are exposed to multiple brewing-associated stresses, such as increased osmotic pressure, enhanced alcohol concentration and nutritional imbalance. These will speed up yeast autolysis, which seriously influence beer flavor and quality. To increase yeast anti-autolytic ability, FKS1 overexpression strain was constructed by 18S rDNA. The concentration of β-1,3-glucan of overexpression strain was 62% higher than that of wild type strain. Meantime, FKS1 overexpression strain increased anti-stress ability at 8% ethanol, 0.4 mol/L NaCl and starvation stress. Under simulated autolysis, FKS1 showed good anti-autolytic ability by slower autolysis. These results confirms the potential of FKS1 overexpression to tackle yeast autolysis in high-gravity brewing.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Jinjing
authors.initials: J
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
mesh_terms: D001329:Autolysis; D001515:Beer; D054714:Echinocandins / Q000235:genetics*; D005964:Glucosyltransferases / Q000235:genetics*; D018471:Hypergravity; D008565:Membrane Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
chemical_list: D054714:Echinocandins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D005964:Glucosyltransferases; C091618:FKS1 protein, S cerevisiae