title: Decellularized extracellular matrix microparticles as a vehicle for cellular delivery in a model of anastomosis healing.
abstract: Extracellular matrix (ECM) materials from animal and human sources have become important materials for soft tissue repair. Microparticles of ECM materials have increased surface area and exposed binding sites compared to sheet materials. Decellularized porcine peritoneum was mechanically dissociated into 200 µm microparticles, seeded with fibroblasts and cultured in a low gravity rotating bioreactor. The cells avidly attached and maintained excellent viability on the microparticles. When the seeded microparticles were placed in a collagen gel, the cells quickly migrated off the microparticles and through the gel. Cells from seeded microparticles migrated to and across an in vitro anastomosis model, increasing the tensile strength of the model. Cell seeded microparticles of ECM material have potential for paracrine and cellular delivery therapies when delivered in a gel carrier. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1728-1735, 2016.
authors.affiliation: Department of Cardiac Surgery, Boston Children's Hospital, Boston, Massachusetts.
authors.forename: David M
authors.initials: DM
authors.lastname: Hoganson
authors.affiliation: Graduate Option in Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, California.
authors.forename: Gwen E
authors.initials: GE
authors.lastname: Owens
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Meppelink
authors.affiliation: Department of Surgery, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Erik K
authors.initials: EK
authors.lastname: Bassett
authors.affiliation: DSM Biomedical, Exton, Pennsylvania.
authors.forename: Chris M
authors.initials: CM
authors.lastname: Bowley
authors.affiliation: Department of Biomedical Engineering, Washington University in St. Louis, Missouri.
authors.forename: Cameron J
authors.initials: CJ
authors.lastname: Hinkel
authors.affiliation: Department of Biomedical and Chemical Engineering, Syracuse Biomaterials Institute, Syracuse University, Syracuse, New York.
authors.forename: Eric B
authors.initials: EB
authors.lastname: Finkelstein
authors.affiliation: DSM Biomedical, Exton, Pennsylvania.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Goldman
authors.affiliation: Department of Surgery, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Vacanti
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002465:Cell Movement; D003094:Collagen; D016503:Drug Delivery Systems / Q000379:methods*; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008863:Microspheres; D008954:Models, Biological; D034421:Sus scrofa; D013718:Tensile Strength; D014945:Wound Healing
chemical_list: D003094:Collagen
keywords: 3-D cell culture; cell adhesion; cell migration; extracellular matrix; microparticles