title: Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice.
abstract: AGEs are a heterogeneous group of molecules formed from the nonenzymatic reaction of reducing sugars with free amino groups of proteins, lipids, and/or nucleic acids. AGEs have been shown to play a role in various conditions including cardiovascular disease and diabetes. In this study, we hypothesized that AGEs play a role in the "multiple hit hypothesis" of nonalcoholic fatty liver disease (NAFLD) and contribute to the pathogenesis of hepatosteatosis. We measured the effects of various mouse chows containing high or low AGE in the presence of high or low fat content on mouse weight and epididymal fat pads. We also measured the effects of these chows on the inflammatory response by measuring cytokine levels and myeloperoxidase activity levels on liver supernatants. We observed significant differences in weight gain and epididymal fat pad weights in the high AGE-high fat (HAGE-HF) versus the other groups. Leptin, TNF-α, IL-6, and myeloperoxidase (MPO) levels were significantly higher in the HAGE-HF group. We conclude that a diet containing high AGEs in the presence of high fat induces weight gain and hepatosteatosis in CD-1 mice. This may represent a model to study the role of AGEs in the pathogenesis of hepatosteatosis and steatohepatitis.
authors.affiliation: Digestive Diseases, Hepatology and Nutrition Center, Connecticut Children's Medical Center, Hartford, CT 06106, USA; University of Connecticut School of Medicine, Farmington, CT 06032, USA.
authors.forename: Wael N
authors.initials: WN
authors.lastname: Sayej
authors.affiliation: Department of Anesthesiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY 14214, USA; Veterans Administration Western New York Healthcare System, State University of New York at Buffalo, Buffalo, NY 14215, USA; Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY 14214, USA.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Knight Iii
authors.affiliation: Department of Surgery, University at Buffalo-SUNY School of Medicine, Buffalo, NY 14214, USA.
authors.forename: Weidun Alan
authors.initials: WA
authors.lastname: Guo
authors.affiliation: Department of Anesthesiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY 14214, USA; Departments of Pathology and Anatomical Sciences, School of Medicine and Biomedical Sciences, Buffalo, NY 14228, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Mullan
authors.affiliation: Department of Rehabilitation Science, University at Buffalo-SUNY School of Medicine, Buffalo, NY 14214, USA.
authors.forename: Patricia J
authors.initials: PJ
authors.lastname: Ohtake
authors.affiliation: Department of Anesthesiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY 14214, USA; Veterans Administration Western New York Healthcare System, State University of New York at Buffalo, Buffalo, NY 14215, USA; Departments of Pathology and Anatomical Sciences, School of Medicine and Biomedical Sciences, Buffalo, NY 14228, USA.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Davidson
authors.affiliation: Department of Pediatric Pathology, Women & Children's Hospital of Buffalo, Buffalo, NY 14203, USA.
authors.forename: Abdur
authors.initials: A
authors.lastname: Khan
authors.affiliation: Department of Pediatric Pathology, Women & Children's Hospital of Buffalo, Buffalo, NY 14203, USA.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Baker
authors.affiliation: Digestive Diseases and Nutrition Center, Division of Pediatric Gastroenterology, Women & Children's Hospital of Buffalo, Buffalo, NY 14222, USA.
authors.forename: Susan S
authors.initials: SS
authors.lastname: Baker
mesh_terms: D000273:Adipose Tissue / Q000503:physiopathology; D000818:Animals; D059305:Diet, High-Fat; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015850:Interleukin-6 / Q000096:biosynthesis; D020738:Leptin / Q000096:biosynthesis; D008099:Liver / Q000378:metabolism / Q000473:pathology; D051379:Mice; D065626:Non-alcoholic Fatty Liver Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009765:Obesity / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015430:Weight Gain / Q000235:genetics
chemical_list: D017127:Glycation End Products, Advanced; D015850:Interleukin-6; D020738:Leptin; D014409:Tumor Necrosis Factor-alpha; D009195:Peroxidase