title: [Breeding of high-producing LI-F lipopeptide Paenibacillus polymyxa by protoplast fusion and differential expression analysis of fusion strains].
abstract: Auxotrophic strains of N1-37 (Phe-) and N2-27 (His-), screened from mutations of Paenibacillus polymyxa JSa-9 previously, were used as the parent strains to screen high-producing LI-F antibacterial lipopeptide fusion strain through protoplast fusion with polyethylene glycol as a promote agent. Fusion strain F5-15 was obtained. Then the product of LI-F antibacterial lipopeptide was quantified by HPLC, and the difference of expression of the key genes of lipopeptide synthase between wild strain JSa-9 and the fusion strain was analyzed by real-time PCR. LI-F antibacterial lipopeptide yield of the fusion strain F5-15 was 3.1-fold of the original strain JSa9's, and the expression levels of the target genes were 10.48, 2.48, 2.1 and 11.8 fold of the initial strain JSa-9, respectively.
authors.forename: Dong
authors.initials: D
authors.lastname: Yan
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Han
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bie
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Lu
authors.forename: Fengxia
authors.initials: F
authors.lastname: Lü
authors.forename: Haizhen
authors.initials: H
authors.lastname: Zhao
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D002851:Chromatography, High Pressure Liquid; D055666:Lipopeptides / Q000096:biosynthesis*; D056507:Paenibacillus / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D055666:Lipopeptides