title: IMPROVED SURVIVAL AND DEVELOPMENTAL RATES IN VITRIFIED-WARMED PIG OOCYTES AFTER RECOVERY CULTURE WITH COENZYME Q10.
abstract: BACKGROUND
  The primary problems with porcine oocyte vitrification are their low viability and development; both need improvement.
  
  
  OBJECTIVE
  This study was designed to improve the survival and developmental rates in vitrified-warmed porcine oocytes.
  
  
  METHODS
  Porcine oocytes matured in vitro were vitrified-warmed with Cryotop. Then the oocytes were supplemented with Q10 during recovery culture.
  
  
  RESULTS
  The survival rates immediately after warming were 92.9% by morphological inspection and 39.3% by fluorescein diacetate (FDA) assay. The group of recovery culture with Q10 (VC+Q10) showed significantly higher viability compared to the group of recovery culture without Q10 (VC+) analyzed by morphology and the FDA. The VC+Q10 group showed a low Bax/Bcl-xl ratio and a high expression of MAP3K12 and TGFB3 compared to the VC+. The cleavage rate did not differ in both groups but, blastocyst yield was higher in VC+Q10 than the VC+ group.
  
  
  CONCLUSIONS
  Supplementation of Q10 during recovery culture led to a higher blastocyst yield by increasing survival rates and regulating mRNA expressions.
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeollabuk-do, Republic of Korea.
authors.forename: In-Sul
authors.initials: IS
authors.lastname: Hwang
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeollabuk-do, Republic of Korea.
authors.forename: D ae-Jin
authors.initials: Da
authors.lastname: Kwon
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeollabuk-do, Republic of Korea.
authors.forename: Tae-Uk
authors.initials: TU
authors.lastname: Kwak
authors.affiliation: Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea.
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeollabuk-do, Republic of Korea.
authors.forename: Gi-Sun
authors.initials: GS
authors.lastname: Im
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Jeollabuk-do, Republic of Korea. hwangss@korea.kr.
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Hwang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D009865:Oocytes; D034421:Sus scrofa / Q000502:physiology*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D058989:Vitrification
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D014451:Ubiquinone; C024989:coenzyme Q10