title: Expression profile and prognostic value of NNMT in patients with pancreatic cancer.
abstract: The elevation of Nicotinamide N-methyltransferase (NNMT) has been reported in pancreatic cancer tissues and cell lines, but its clinical and prognostic implications remain controversial. This study aimed at investigating the expression of NNMT in pancreatic benign and malignant tissues and the prognostic value of NNMT in pancreatic cancer. The expression of NNMT in tissue specimens of 28 chronic pancreatitis patients and 178 pancreatic cancer patients were assayed with immunohistochemistry on tissue microarray. The NNMT expression levels of pancreatic patients were correlated with their clinicopathological characteristics. The influences of NNMT expression and patients' clinicopathological characteristics on overall survival (OS) were analyzed. The percentage of NNMT high expression (NNMTh) in pancreatic cancer (55.6%) was significantly higher than those in chronic pancreatitis (21.4%) and paracancerous tissues (14.8%) (p < 0.001). NNMTh tends to significantly correlate with unfavorable clinicopathological features such as age > 60 years old (p = 0.014), tumor diameter > 4 cm (p < 0.001), TNM stage III or IV (p < 0.001) and poor tumor differentiation (p = 0.004). The median OS of patients with NNMTh and NNMTl were 7.0 months (95% CI: 5.275-8.725) and 11.5 months (95% CI: 9.759-13.241) respectively (p = 0.005). On multivariate analysis, NNMTl (hazards ratio [HR]: 0.399; 95% CI: 0.284-0.560; p < 0.001), absence of neurological involvement (HR: 0.651; 95% CI: 0.421-0.947; p = 0.041), TNM stage I or II (HR: 0.506; 95% CI: 0.299-0.719; p = 0.015) and well tumor differentiation (HR: 0.592; 95% CI: 0.319-0.894; p = 0.044) were significant favorable prognostic factors of OS. In conclusion, NNMT is upregulated in pancreatic cancer, correlates with unfavorable clinicopathological features and may serve as an independent prognosticator of patients' survival.
authors.affiliation: Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, China.|Department of Nephrology, The Affiliated Huai'an Hospital of Xuzhou Medical College, Huai'an, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pathology, Shiyan Taihe Hospital, Hubei University of Medicine, Shiyan City, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Nephrology, The Affiliated Huai'an Hospital of Xuzhou Medical College, Huai'an, China.
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Zheng
authors.affiliation: Department of Pathology, Jinling Hospital, Nanjing, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: Department of Pathology, The Affiliated Huai'an Hospital of Xuzhou Medical College, Huai'an, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Changying
authors.initials: C
authors.lastname: Xing
authors.affiliation: Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing, China.|Huadong Medical Institute of Biotechniques, Nanjing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D050903:Nicotinamide N-Methyltransferase / Q000378:metabolism*; D010180:Pancreatectomy; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D050500:Pancreatitis, Chronic / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D011379:Prognosis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C494406:NNMT protein, human; D050903:Nicotinamide N-Methyltransferase
keywords: clinicopathological features; nicotinamide N-methyltransferase; overall survival; pancreatic cancer