title: Selenium and its compounds in aquatic plant Veronica anagallis-aquatica.
abstract: The uptake, distribution and determination of Se and its compounds in macrophyte Veronica anagallis-aquatica were investigated. V. anagallis-aquatica and sediments were sampled in years 2009-2011 and in 2013 in three Slovenian watercourses flowing through an agricultural area, where addition of Se in feedstuffs has been performed for about 25 years. Se content in sediments were up to 0.86 μg g(-1) and in whole plant varied from 0.186 to 1.535 μg g(-1), all on dry weight basis. Se content were measured also in different plant parts; highest content were found in roots and lowest in stems. Separation of extractable Se compounds was performed by ion exchange chromatography and for on-line detection inductively coupled plasma-mass spectrometry was used. The results showed that only approximately 24% of Se in the macrophyte was extracted using enzyme Protease XIV. Extractable Se in plant parts varied from 10.5% in roots to 29.6% in leaves. Identification of Se(IV) and Se(VI) was achieved but no Se-amino acids were detected even at highest Se content. According to our results, we assume that 25 years of Se addition in feedstuff shows minimal impact on Se content in the selected agricultural area.
authors.affiliation: "Jožef Stefan" Institute, Jamova cesta 39, SI-1000 Ljubljana, Slovenia; Jožef Stefan International Postgraduate School, Jamova cesta 39, SI-1000 Ljubljana, Slovenia.
authors.forename: Ana
authors.initials: A
authors.lastname: Kroflič
authors.affiliation: Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
authors.forename: Mateja
authors.initials: M
authors.lastname: Germ
authors.affiliation: Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
authors.forename: Špela
authors.initials: Š
authors.lastname: Mechora
authors.affiliation: "Jožef Stefan" Institute, Jamova cesta 39, SI-1000 Ljubljana, Slovenia; Jožef Stefan International Postgraduate School, Jamova cesta 39, SI-1000 Ljubljana, Slovenia. Electronic address: vekoslava.stibilj@ijs.si.
authors.forename: Vekoslava
authors.initials: V
authors.lastname: Stibilj
mesh_terms: D002852:Chromatography, Ion Exchange; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000254:growth & development; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D012621:Seasons; D012643:Selenium / Q000032:analysis*; D018036:Selenium Compounds / Q000032:analysis*; D017524:Slovenia; D032264:Veronica / Q000737:chemistry* / Q000254:growth & development; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D018036:Selenium Compounds; D014874:Water Pollutants, Chemical; D012643:Selenium
keywords: Agricultural area; River sediments; Selenium compounds; Veronica anagallis-aquatica