title: Hypercrosslinked strong cation-exchange polymers for selective extraction of serum purine metabolites associated with gout.
abstract: In this study, hypercrosslinked strong cation-exchange polymer resins (HXLPP-SCX) were synthesized and employed as selective sorbents for the solid-phase extraction (SPE) of basic purine metabolites associated with gout. The HXLPP-SCX material was prepared based on hypercrosslinking reactions and sulfonated with concentrated H2SO4. This synthetic procedure is facile and efficient without using highly toxic reagent. The resulting resins were characterized in the form of monodisperse microspheres (mean diameters of 3‒5μm) with narrow pore size (2.1nm) and relatively high specific surface areas (801m(2)/g). The polymers also possess high ion-exchange capacity (IEC, 2.22mmol/g) and good adsorption and selectivity performances for basic compounds. The resins used as SPE sorbents permit the selective enrichment of three pivotal purine metabolites (hypoxanthine, xanthine and inosine) in human serum followed by HPLC analysis. Method validation including linearity range, sensitivity, accuracy and reproducibility were evaluated. This method was exemplarily applied in the analysis of serum purines in gout patients and healthy controls. The present results demonstrate a promising potential of this HXLPP-SCX material for the clinical sample pretreatment.
authors.affiliation: Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Rheumatism, Jiujiang First People's Hospital, Affiliated Jiujiang Hospital of Nanchang University, Jiujiang 332000, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: Hongyang_Zhang@ecust.edu.cn.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Rheumatism, Jiujiang First People's Hospital, Affiliated Jiujiang Hospital of Nanchang University, Jiujiang 332000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Internal Medicine, Jiujiang First People's Hospital, Affiliated Jiujiang Hospital of Nanchang University, Jiujiang 332000, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Cao
authors.affiliation: Shanghai Key Laboratory of New Drug Design & Modern Engineering Center for TCM, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Ruan
authors.affiliation: Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: Huping@ecust.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
mesh_terms: D002411:Cation Exchange Resins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D006073:Gout / Q000097:blood* / Q000175:diagnosis; D006801:Humans; D019271:Hypoxanthine / Q000097:blood / Q000737:chemistry; D007288:Inosine / Q000097:blood / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D011687:Purines / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D014527:Uric Acid / Q000097:blood; D019820:Xanthine / Q000097:blood / Q000737:chemistry
chemical_list: D002411:Cation Exchange Resins; D003432:Cross-Linking Reagents; D011108:Polymers; D011687:Purines; D019820:Xanthine; D014527:Uric Acid; D019271:Hypoxanthine; D007288:Inosine
keywords: Basic purine metabolites; Gout; Human serum; Hypercrosslinked strong cation-exchange polymers; Solid-phase extraction