title: Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection.
abstract: Helicobacter pylori cause chronic inflammation favouring gastric carcinogenesis, and its eradication may prevent malignant transformation. We evaluated whether H. pylori infection and its eradication modify the expression of inflammatory mediators in patients with chronic gastritis. Furthermore, we assessed whether microRNAs modulate inflammatory pathways induced by H. pylori and identified miRNA-gene interaction networks. mRNA and protein expression of TNFA, IL6, IL1B, IL12A, IL2 and TGFBRII and miRNAs miR-103a-3p, miR-181c-5p, miR-370-3p, miR-375 and miR-223-3p were evaluated in tissue samples from 20 patients with chronic gastritis H. pylori negative (Hp-) and 31 H. pylori positive (Hp+), before and three months after bacterium eradication therapy, in comparison with a pool of Hp- normal gastric mucosa. Our results showed that H. pylori infection leads to up-regulation of TNFA, IL6, IL12A and IL2 and down-regulation of miRNAs. Bacterium eradication reduces the expression of TNFA and IL6 and up-regulates TGFBRII and all investigated miRNAs, except miR-223-3p. Moreover, transcriptional profiles of inflammatory mediators and miRNAs after eradication are different from the non-infected group. Deregulated miRNA-mRNA interaction networks were observed in the Hp+ group before and after eradication. Therefore, miRNAs modulated cytokine expression in the presence of H. pylori and after its eradication, suggesting that miRNAs participate in the pathological process triggered by H. pylori in the gastric mucosa.
authors.affiliation: UNESP, São Paulo State University, Department of Biology, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, Brazil.
authors.forename: Ana Flávia Teixeira
authors.initials: AF
authors.lastname: Rossi
authors.affiliation: UNESP, São Paulo State University, Department of Biology, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, Brazil.
authors.forename: Aline Cristina Targa
authors.initials: AC
authors.lastname: Cadamuro
authors.affiliation: UNESP, São Paulo State University, Department of Biology, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, Brazil.
authors.forename: Joice Matos
authors.initials: JM
authors.lastname: Biselli-Périco
authors.affiliation: USP, São Paulo University, Faculty of Medicine, Department of Surgery, Avenida Dr. Arnaldo, 455, São Paulo, SP, Brazil.
authors.forename: Kátia Ramos Moreira
authors.initials: KR
authors.lastname: Leite
authors.affiliation: UNESP, São Paulo State University, Faculty of Medicine, Department of Surgery and Orthopedics, Avenida Prof. Montenegro, Botucatu, SP, Brazil.
authors.forename: Fábio Eduardo
authors.initials: FE
authors.lastname: Severino
authors.affiliation: UNESP, São Paulo State University, Faculty of Medicine, Department of Surgery and Orthopedics, Avenida Prof. Montenegro, Botucatu, SP, Brazil.
authors.forename: Patricia P
authors.initials: PP
authors.lastname: Reis
authors.affiliation: UNESP, São Paulo State University, Department of Biology, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, Brazil.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Cordeiro
authors.affiliation: UNESP, São Paulo State University, Department of Biology, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, Brazil. anabete@ibilce.unesp.br.
authors.forename: Ana Elizabete
authors.initials: AE
authors.lastname: Silva
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D005756:Gastritis / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D016481:Helicobacter Infections / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D055815:Young Adult
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D035683:MicroRNAs