title: Proteomics study reveals that the dysregulation of focal adhesion and ribosome contribute to early pregnancy loss.
abstract: Early pregnancy loss (EPL) affects 50-70% pregnant women in first trimester. The precise molecular mechanisms underlying EPL are far from being fully understood. Therefore, we aim to identify the molecular signaling pathways relating to EPL.
  
  
  
  We performed proteomics and bioinformatics analysis of the placental villi in women who have undergone EPL and in normal pregnant women. The proteomics data were validated by Western blot analysis.
  
  
  
  We identified a total of 5952 proteins in placental villi, of which 588 proteins were differentially expressed in the EPL women. Bioinformatics analysis revealed that these differentially expressed proteins participated in a variety of signaling pathways, including the focal adhesion pathway and ribosome pathway. Moreover, results of the Western blot confirmed that Desmin, Lamin A/C, MMP-9, and histone H4 were upregulated in EPL and the Lamin C/ Lamin A ratio decreased obviously in EPL. These proteins could be associated with the pathophysiology of EPL. The data have been deposited to the ProteomeXchange with identifier PXD002391.
  
  
  
  Our study demonstrated that the focal adhesion pathway and ribosome pathway are involved in EPL, and these findings might contribute to unveil the pathophysiology of EPL.
authors.affiliation: Department of Obstetrics and Gynecology, the General Hospital of the PLA Rocket Force, Beijing, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Xin
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Benhong
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, the General Hospital of the PLA Rocket Force, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Obstetrics and Gynecology, the General Hospital of the PLA Rocket Force, Beijing, China.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Hou
authors.affiliation: Department of Obstetrics and Gynecology, the General Hospital of the PLA Rocket Force, Beijing, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ye
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Meng
authors.affiliation: Department of Obstetrics and Gynecology, the General Hospital of the PLA Rocket Force, Beijing, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ding
authors.affiliation: National Key Laboratory of Medical Molecular Biology, Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
mesh_terms: D000022:Abortion, Spontaneous / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000378:metabolism / Q000473:pathology; D003893:Desmin / Q000235:genetics / Q000378:metabolism; D005260:Female; D022001:Focal Adhesions / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D034904:Lamin Type A / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D040901:Proteomics / Q000379:methods; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction
chemical_list: D015415:Biomarkers; D003893:Desmin; D006657:Histones; C494847:LMNA protein, human; D034904:Lamin Type A; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Bioinformatics analysis; Early pregnancy loss; Placental villi; Proteomics analysis