title: Enhanced performance for the analysis of prostaglandins and thromboxanes by liquid chromatography-tandem mass spectrometry using a new atmospheric pressure ionization source.
abstract: Eicosanoids, including prostaglandins and thromboxanes are lipid mediators synthetized from polyunsaturated fatty acids. They play an important role in cell signaling and are often reported as inflammatory markers. LC-MS/MS is the technique of choice for the analysis of these compounds, often in combination with advanced sample preparation techniques. Here we report a head to head comparison between an electrospray ionization source (ESI) and a new atmospheric pressure ionization source (UniSpray). The performance of both interfaces was evaluated in various matrices such as human plasma, pig colon and mouse colon. The UniSpray source shows an increase in method sensitivity up to a factor 5. Equivalent to better linearity and repeatability on various matrices as well as an increase in signal intensity were observed in comparison to ESI.
authors.affiliation: Discovery Sciences, Janssen R&D, Beerse, Belgium.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lubin
authors.affiliation: Discovery Sciences, Janssen R&D, Beerse, Belgium.
authors.forename: Suzy
authors.initials: S
authors.lastname: Geerinckx
authors.affiliation: Waters Corporation, Wilmslow, United Kingdom.
authors.forename: Steve
authors.initials: S
authors.lastname: Bajic
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, Belgium.
authors.forename: Deirdre
authors.initials: D
authors.lastname: Cabooter
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, Belgium.
authors.forename: Patrick
authors.initials: P
authors.lastname: Augustijns
authors.affiliation: Discovery Sciences, Janssen R&D, Beerse, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Cuyckens
authors.affiliation: Discovery Sciences, Janssen R&D, Beerse, Belgium. Electronic address: rvreeken@its.jnj.com.
authors.forename: Rob J
authors.initials: RJ
authors.lastname: Vreeken
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards*; D003106:Colon / Q000737:chemistry; D015777:Eicosanoids / Q000032:analysis*; D006801:Humans; D051379:Mice; D011453:Prostaglandins / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D013552:Swine; D053719:Tandem Mass Spectrometry; D013931:Thromboxanes / Q000032:analysis* / Q000097:blood
chemical_list: D015777:Eicosanoids; D011453:Prostaglandins; D013931:Thromboxanes
keywords: Eicosanoid; Electrospray; LC–MS/MS; Prostaglandin; Unispray