title: Benzo(a)pyrene induces interleukin (IL)-6 production and reduces lipid synthesis in human SZ95 sebocytes via the aryl hydrocarbon receptor signaling pathway.
abstract: In this study, we determined the effects of benzo(a)pyrene (BaP) on the expression of aryl hydrocarbon receptor (AhR) and cytochrome P450 1A1 (CYP1A1), and assessed the action of BaP on inflammatory cytokine expression and lipid synthesis in SZ95 sebocytes in vitro. BaP (10(-8), 10(-7), 10(-6) and 10(-5)M) was not cytotoxic for SZ95 sebocytes after 24h exposure. Expression of AhR was promoted in mRNA lever, while was inhibited in protein lever after BaP (10(-5)M) exposure. CYP1A1 expression was up-regulated in both mRNA and protein levels. BaP (10(-5)M) exerted a stimulatory action on interleukin (IL)-6 secretion, while a dose-dependently inhibitory effect on lipid synthesis from 10(-8)M to 10(-5)M in SZ95 sebocytes. Both actions were partly antagonized in AhR-knockdowned SZ95 sebocytes. This study demonstrates that BaP can activate AhR signaling pathway, and exhibits pro-inflammatory effects and inhibitory effects on sebum production in human sebocytes.
authors.affiliation: Department of Dermatology, Shanghai Dermatology Hospital, Clinical Academy of Anhui Medical University, Shanghai, 200443, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Dermatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, PR China.
authors.forename: Zhanyan
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Department of Dermatology, Shanghai Dermatology Hospital, Clinical Academy of Anhui Medical University, Shanghai, 200443, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Dermatology, Shanghai Dermatology Hospital, Clinical Academy of Anhui Medical University, Shanghai, 200443, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Mo
authors.affiliation: Department of Dermatology, Shanghai Dermatology Hospital, Clinical Academy of Anhui Medical University, Shanghai, 200443, PR China.
authors.forename: Ningjing
authors.initials: N
authors.lastname: Song
authors.affiliation: Department of Dermatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.affiliation: Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, 14195, Germany.
authors.forename: Christos C
authors.initials: CC
authors.lastname: Zouboulis
authors.affiliation: Department of Dermatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, PR China.
authors.forename: Longqing
authors.initials: L
authors.lastname: Xia
authors.affiliation: Department of Dermatology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200127, PR China. Electronic address: qiangju@aliyun.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ju
mesh_terms: D001564:Benzo(a)pyrene / Q000633:toxicity*; D002460:Cell Line; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D015386:Hazardous Substances / Q000633:toxicity*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D050155:Lipogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*
chemical_list: D015386:Hazardous Substances; D015850:Interleukin-6; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D001564:Benzo(a)pyrene; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1
keywords: Acne; Acne inversa; AhR; Benzo(a)pyrene; IL-6; SZ95 sebocytes