title: CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering.
abstract: The advent of genome editing techniques based on the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system has revolutionized research in the biological sciences. CRISPR is quickly becoming an indispensible experimental tool for researchers using genetic model organisms, including the nematode Caenorhabditis elegans. Here, we provide an overview of CRISPR-based strategies for genome editing in C. elegans. We focus on practical considerations for successful genome editing, including a discussion of which strategies are best suited to producing different kinds of targeted genome modifications.
authors.affiliation: Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280 ddickins@live.unc.edu.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Dickinson
authors.affiliation: Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280.
authors.forename: Bob
authors.initials: B
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D004260:DNA Repair; D005818:Genetic Engineering / Q000379:methods*; D016297:Mutagenesis, Site-Directed
keywords: CRISPR/Cas9; Caenorhabditis elegans; WormBook; genome editing