title: High Numbers of Stromal Cancer-Associated Fibroblasts Are Associated With a Shorter Survival Time in Cats With Oral Squamous Cell Carcinoma.
abstract: Cancer-associated fibroblasts (CAFs) are fibroblastic cells that express α-smooth muscle actin and have been identified in the stroma of numerous epithelial tumors. The presence of CAFs within the tumor stroma has been associated with a poorer prognosis in some human cancers, including oral squamous cell carcinomas (SCCs). Cats frequently develop oral SCCs, and although these are generally highly aggressive neoplasms, there is currently a lack of prognostic markers for these tumors. The authors investigated the prognostic value of the presence of CAFs within the stroma of oral SCC biopsy specimens from 47 cats. In addition, several epidemiologic, clinical, and histologic variables were also assessed for prognostic significance. A CAF-positive stroma was identified in 35 of 47 SCCs (74.5%), and the median survival time (ST) of cats with CAF-positive SCCs (35 days) was significantly shorter than that of cats with CAF-negative SCCs (48.5 days) (P = .031). ST was also associated with the location of the primary tumor (P = .0018): the median ST for oropharyngeal SCCs (179 days) was significantly longer than for maxillary (43.5 days; P = .047), mandibular (42 days; P = .022), and sublingual SCCs (22.5 days; P = .0005). The median ST of sublingual SCCs was also shorter compared with maxillary SCCs (P = .0017). Furthermore, a significant association was identified between site and the presence of stromal CAFs (P = .025). On the basis of this retrospective study, evaluating the tumor stroma for CAFs in feline oral SCC biopsy specimens may be of potential prognostic value.
authors.affiliation: Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand h.j.klobukowska@massey.ac.nz.
authors.forename: H J
authors.initials: HJ
authors.lastname: Klobukowska
authors.affiliation: Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.
authors.forename: J S
authors.initials: JS
authors.lastname: Munday
mesh_terms: D000818:Animals; D001706:Biopsy / Q000662:veterinary; D000072645:Cancer-Associated Fibroblasts / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D002371:Cat Diseases / Q000175:diagnosis / Q000401:mortality / Q000473:pathology*; D002415:Cats; D005260:Female; D008297:Male; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000401:mortality / Q000473:pathology / Q000662:veterinary*; D011379:Prognosis; D016019:Survival Analysis
keywords: alpha-smooth muscle actin; cancer-associated fibroblast; cat; myofibroblast; oncology; oral; prognosis; squamous cell carcinoma