title: Degradation of olive mill wastewater by the induced extracellular ligninolytic enzymes of two wood-rot fungi.
abstract: Olive mill wastewater (OMWW) is a major problem in olive oil - producing countries, due to its high organic load and concentration in phenols that are toxic for marine life, plants and soil microorganisms. In the present study, two mushroom species were tested in regard to their OMWW's oxidative capacity, Pleurotus citrinopileatus LGAM 28684 and Irpex lacteus LGAM 238. OMWW (25% v/v) degradation was investigated for several culture conditions, namely pH, agitation speed, nitrogen-based supplements and their concentration. The selected values were pH 6, agitation rate 150 rpm, 30 g L-1corn steep liquor as nitrogen source for P. citrinopileatus and 20 g L-1diammonium tartrate for I. lacteus. The two strains performed well in cultures supplemented with OMWW, generating very high titers of oxidative enzymes and achieving more than 90% color and phenols reduction within a 24 days cultivation period. In addition, the amount of glucans present in the fungal biomass was assessed. Hence, P. citrinopileatus and I. lacteus appear as potent degraders of OMWW with the ability to use the effluent as a substrate for the production of biotechnologically important enzymes and valuable fungal glucans.
authors.affiliation: Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, Athens 15780, Greece.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Zerva
authors.affiliation: Agricultural University of Athens, Laboratory of General and Agricultural Microbiology, Iera Odos 75, 11855 Athens, Greece.
authors.forename: Georgios I
authors.initials: GI
authors.lastname: Zervakis
authors.affiliation: Biochemical and Chemical Process Engineering, Division of Sustainable Process Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-97187 Luleå, Sweden.
authors.forename: Paul
authors.initials: P
authors.lastname: Christakopoulos
authors.affiliation: Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, Athens 15780, Greece. Electronic address: vtopakas@chemeng.ntua.gr.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Topakas
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D031658:Olea; D000069463:Olive Oil; D010636:Phenols; D062065:Wastewater; D014934:Wood
chemical_list: D000069463:Olive Oil; D010636:Phenols; D062065:Wastewater
keywords: Biodegradation; Fungal glucan; Irpex lacteus; Ligninolytic enzymes; Pleurotus citrinopileatus