title: A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection.
abstract: A non-derivative method was developed for the qualitative and quantitative analysis of isosteroidal alkaloids from Fritillaria thunbergii. During method development the performance of two universal detectors, the charged aerosol detector (CAD) and evaporative light scattering detector (ELSD), were evaluated. The CAD was found to be 30 to 55 times more sensitive than ELSD enabling the measurement of low levels of reference compound impurities that could not be detected by ELSD. The peak area percent of the reference compound, peimisine, obtained by CAD was 50.10%, but 91.66% by ELSD showing that CAD is suitable to estimate the presence of impurities. The CAD showed good reproducibility with overall intra- and inter-day peak area RSD values of less than 1.8% and 2.7%, respectively and had a linear dynamic range of up to 4 orders of magnitude (0.06-44mg/L) for peimine and peiminine. The optimized method was used for the quantitative analysis of peimine and peiminine from F. thunbergii.
authors.affiliation: Thermofisher Scientific Corporation, Beijing 100080, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Long
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: guozhimou@dicp.ac.cn.
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Thermofisher Scientific Corporation, Chelmsford 01824, USA.
authors.forename: Ian N
authors.initials: IN
authors.lastname: Acworth
authors.affiliation: Thermofisher Scientific Corporation, Beijing 100080, China.
authors.forename: Xiaoda
authors.initials: X
authors.lastname: Liu
authors.affiliation: Thermofisher Scientific Corporation, Beijing 100080, China. Electronic address: yan.jin@thermofisher.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Thermofisher Scientific Corporation, Beijing 100080, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Liu
authors.affiliation: Thermofisher Scientific Corporation, Beijing 100080, China.
authors.forename: Lvye
authors.initials: L
authors.lastname: Liu
authors.affiliation: Thermofisher Scientific Corporation, Beijing 100080, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liang
mesh_terms: D000336:Aerosols / Q000032:analysis*; D000470:Alkaloids / Q000032:analysis*; D002595:Cevanes / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031410:Fritillaria / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000336:Aerosols; D000470:Alkaloids; D002595:Cevanes; C532925:hupehenine; C052510:peimisine; C014242:verticine; C047331:peiminine
keywords: Charged aerosol detector; Evaporative light scattering detector; Isosteroidal alkaloids; Quantitative; Weak UV absorbance compound