title: Hierarchical nanostructures of Au@ZnO: antibacterial and antibiofilm agent.
abstract: The perpetual use of antibiotics against pathogens inadvertently altered their genes that have translated into an unprecedented resistance in microorganisms in the twenty-first century. Many researchers have formulated bactericidal and bacteriostatic inorganic nanoparticle-based antiseptics that may be linked to broad-spectrum activity and far lower propensity to induce microbial resistance than organic-based antibiotics. Based on this line, herein, we present observations on microbial abatement using gold-based zinc oxide nanostructures (Au@ZnO) which are synthesized using hydrothermal route. Inhibition of microbial growth and biofilm using Au@ZnO is a unique feature of our study. Furthermore, this study evinces antimicrobial and antibiofilm mechanisms of photo-eradiated Au@ZnO by disruption of cellular functions and biofilms via reactive oxygen species (ROS)-dependent generation of superoxide anion radical. The present study is significant as it introduces novel functionalities to Au@ZnO in the biomedical field which can be extended to other species of microbial pathogens.
authors.affiliation: Department of Physics, Fergusson College, Savitribai Phule Pune University, Pune, 411004, India. haribhau.gholap@fergusson.edu.
authors.forename: Haribhau
authors.initials: H
authors.lastname: Gholap
authors.affiliation: Department of Physics, Nowrosjee Wadia College, Savitribai Phule Pune University, Pune, 411001, India.
authors.forename: Sambhaji
authors.initials: S
authors.lastname: Warule
authors.affiliation: Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Aurangabad, 431001, India.
authors.forename: Jaiprakash
authors.initials: J
authors.lastname: Sangshetti
authors.affiliation: Department of Physics, Savitribai Phule Pune University, Pune, 411007, India.
authors.forename: Gauri
authors.initials: G
authors.lastname: Kulkarni
authors.affiliation: Department of Physics, Savitribai Phule Pune University, Pune, 411007, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Banpurkar
authors.affiliation: Department of Physics, Savitribai Phule Pune University, Pune, 411007, India.
authors.forename: Surekha
authors.initials: S
authors.lastname: Satpute
authors.affiliation: Department of Biotechnology, Savitribai Phule Pune University, Pune, 411007, India. rpatil@unipune.ac.in.
authors.forename: Rajendra
authors.initials: R
authors.lastname: Patil
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D049329:Nanostructures / Q000737:chemistry / Q000382:microbiology* / Q000633:toxicity*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D006046:Gold; D015034:Zinc Oxide
keywords: Antibiofilm; Antimicrobial; Au@ZnO; Hydrothermal; ROS