title: Enhancing biomass and ethanol production by increasing NADPH production in Synechocystis sp. PCC 6803.
abstract: This study demonstrates that increased NADPH production can improve biomass and ethanol production in cyanobacteria. We over-expressed the endogenous zwf gene, which encodes glucose-6-phosphate dehydrogenase of pentose phosphate pathway, in the model cyanobacterium Synechocystis sp. PCC 6803. zwf over-expression resulted in increased NADPH production, and promoted biomass production compared to the wild type in both autotrophic and mixotrophic conditions. Ethanol production pathway including NADPH-dependent alcohol dehydrogenase was also integrated with and without zwf over-expression. Excessive NADPH production by zwf over-expression could improve both biomass and ethanol production in the autotrophic conditions.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea.
authors.forename: Yun-Nam
authors.initials: YN
authors.lastname: Choi
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea; School of Environmental Science and Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea; Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 790-784, Republic of Korea. Electronic address: jmpark@postech.ac.kr.
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
mesh_terms: D018533:Biomass; D000431:Ethanol / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010427:Pentose Phosphate Pathway / Q000235:genetics; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000431:Ethanol; D009249:NADP; D005954:Glucosephosphate Dehydrogenase
keywords: Co-factor engineering; Cyanobacteria; Pentose phosphate pathway; zwf