title: The conglomeration of diagnostic, prognostic and therapeutic potential of serum miR-199a and its association with clinicopathological features in epithelial ovarian cancer.
abstract: Epithelial ovarian cancer (EOC) is the most lethal cause of morbidity and mortality worldwide. miRNA deregulation evinces a remarkable role in ovarian cancer tumorigenesis. miRNA-199a (miR-199a) is known to be involved in cancer development and progression. Although miR-199a has been studied in various cell types, its correlation with clinicopathological features in EOC has not been documented. In this study, we identified the clinicopathological hallmarks which might be perturbed due to the downregulation of serum miR-199a in EOC. Seventy serum samples from histopathologically confirmed EOC patients and 70 controls were collected. Total RNA from serum was isolated by Trizol method, polyadenylated and reverse transcribed into cDNA. Expression level of miR-199a was detected by using miRNA qRT-PCR. Relative expression was determined with matched controls using U6 snRNA as reference. Level of miR-199a expression was compared with distinct clinicopathological features. Expression of miR-199a was found to be significantly downregulated in comparison with matched normal controls. The expression level of miR-199a was found to be significantly associated with tumor stage, lymph node metastasis, and distal metastasis. Receiver operating characteristic (ROC) curve for diagnostic potential yielded significant area under the curve (AUC) with a considerable sensitivity and specificity. ROC curves for prognosis yielded significant AUCs for histological grade, distal metastasis, lymph node status, and survival. Our findings suggest that miR-199a downregulation might be a potential indicator for disease progression promoting the aggressive tumor progression and be identified as a diagnostic marker to predict the prognosis and survival in EOC patients.
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
authors.forename: Mariyam
authors.initials: M
authors.lastname: Zuberi
authors.affiliation: Department of Surgery, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.affiliation: Department of Gynaecology and Obstetrics, Lok Nayak Hospital, New Delhi, 110002, India.
authors.forename: Gauri
authors.initials: G
authors.lastname: Gandhi
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.
authors.forename: P C
authors.initials: PC
authors.lastname: Ray
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India. alpanasaxena@hotmail.com.
authors.forename: Alpana
authors.initials: A
authors.lastname: Saxena
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D000077216:Carcinoma, Ovarian Epithelial; D015536:Down-Regulation; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C531408:mirn199 microRNA, human
keywords: Epithelial ovarian cancer; biomarker; diagnosis; microRNA-199a; prognosis; serum