title: Antibiotic sensitivity pattern in blaNDM-1-positive and carbapenemase-producing Enterobacteriaceae.
abstract: BACKGROUND
  Some studies published in recent time revealed that many bacteria from Enterobacteriaceae group are multi-antibiotic-resistant because of the production enzymes carbapenemase particularly New Delhi metallo-beta-lactamase encoded by gene called blaNDM-1. Looking at public health importance of this issue there is a need for studies at other centers to confirm or refute published findings.
  
  
  OBJECTIVE
  This study was designed with the aim of exploring antibiotic resistance in Enterobacteriaceae group of bacteria and also to explore gene and enzyme responsible for it.
  
  
  METHODS
  Samples of Enterobacteriaceae were collected from wards and outpatient departments. Antibiotic sensitivity was checked by an automated system (VITEK 2 COMPACT). Carbapenemase production was assessed by Modified Hodge Test. Presence of blaNDM-1 was assessed by polymerase chain reaction.
  
  
  METHODS
  Frequency and percentage were used to describe the data. Frequency of sensitivity was compared between carbapenemase producers and noncarbapenemase producers by Fisher's exact test.
  
  
  RESULTS
  Forty-seven percent bacteria were found to be producing carbapenemase enzyme. These bacteria were significantly less sensitive to cefoperazone, cefepime, and amikacin. Among carbapenemase-producing organisms, 3% and 6% were resistant to tigecycline and colistin, respectively. Forty percent bacteria were found to be having blaNDM-1 gene. There was a significant difference between blaNDM-1-positive and blaNDM-1-negative for sensitivity toward cefoperazone + sulbactam, imipenem, meropenem, amikacin, tobramycine, ciprofloxacin, and levofloxacin.
  
  
  CONCLUSIONS
  Presence of carbapenemase enzyme and blaNDM-1 gene is associated with high level of resistance in Enterobacteriaceae group of bacteria and only few antibiotics have good sensitivity for these organisms.
authors.affiliation: Department of Microbiology, Government Medical College, Surat, Gujarat, India.
authors.forename: Summaiya
authors.initials: S
authors.lastname: Mulla
authors.affiliation: Department of Pharmacology, GMERS Medical College, Dharpur, Patan, Gujarat, India.
authors.forename: Jaykaran
authors.initials: J
authors.lastname: Charan
authors.affiliation: Department of Microbiology, Government Medical College, Surat, Gujarat, India.
authors.forename: Sangita
authors.initials: S
authors.lastname: Rajdev
keywords: Antibiotic resistance; Enterobacteriaceae; blaNDM gene; carbapenemase