title: [Collagen quantitation by detection of marker peptides with HPLC-MS].
abstract: A method for quantitation of collagen was established by detecting marker peptide with high performance liquid chromatography-mass spectrometry (HPLC-MS). Theoretical marker peptides were selected by sequence comparison. Bovine collagen type I was digested with trypsin. Marker peptides typical for collagen type I were identified with HPLC-MS. The relationship between the abundance of marker peptides and collagen concentration was established. The results show that GEAGPSGPAGPTGAR and the other 5 peptides showed high resolution during chromatographic separation and high signal intensity during MS analysis. Peptide signal intensity and collagen concentration showed a good linear relationship in the range from 0.1 to 3 mg/mL. Bovine tendon and collagen sponge were used as actual samples and collagen contents were determined as 90.2% and 93.4% respectively. Quantitation of marker peptides of collagen was a feasible method to identify and quantify collagens in medical device research and development.
authors.forename: Kun
authors.initials: K
authors.lastname: Sun
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Kong
authors.forename: Jiyao
authors.initials: J
authors.lastname: Kang
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.forename: Shenghua
authors.initials: S
authors.lastname: Zha
authors.forename: Guifeng
authors.initials: G
authors.lastname: Zhang
authors.forename: Minglin
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D024042:Collagen Type I / Q000032:analysis*; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis*
chemical_list: D024042:Collagen Type I; D010455:Peptides