title: Pyrosequencing the Midgut Transcriptome of the Banana Weevil Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae) Reveals Multiple Protease-Like Transcripts.
abstract: The banana weevil Cosmopolites sordidus is an important and serious insect pest in most banana and plantain-growing areas of the world. In spite of the economic importance of this insect pest very little genomic and transcriptomic information exists for this species. In the present study, we characterized the midgut transcriptome of C. sordidus using massive 454-pyrosequencing. We generated over 590,000 sequencing reads that assembled into 30,840 contigs with more than 400 bp, representing a significant expansion of existing sequences available for this insect pest. Among them, 16,427 contigs contained one or more GO terms. In addition, 15,263 contigs were assigned an EC number. In-depth transcriptome analysis identified genes potentially involved in insecticide resistance, peritrophic membrane biosynthesis, immunity-related function and defense against pathogens, and Bacillus thuringiensis toxins binding proteins as well as multiple enzymes involved with protein digestion. This transcriptome will provide a valuable resource for understanding larval physiology and for identifying novel target sites and management approaches for this important insect pest.
authors.affiliation: Departamento de Producción Agropecuaria, Universidad de Caldas, Manizales, Colombia.
authors.forename: Arnubio
authors.initials: A
authors.lastname: Valencia
authors.affiliation: Department of Entomology, University of Nebraska, Lincoln, NE, 68583, United States of America.
authors.forename: Haichuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Departamento de Producción Agropecuaria, Universidad de Caldas, Manizales, Colombia.
authors.forename: Alberto
authors.initials: A
authors.lastname: Soto
authors.affiliation: Departamento de Producción Agropecuaria, Universidad de Caldas, Manizales, Colombia.
authors.forename: Manuel
authors.initials: M
authors.lastname: Aristizabal
authors.affiliation: Universidad del Atlántico, Barranquilla, Colombia.
authors.forename: Jorge W
authors.initials: JW
authors.lastname: Arboleda
authors.affiliation: Center for Biotechnology, University of Nebraska, Lincoln, NE, 68588, United States of America.
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Eyun
authors.affiliation: Programa de Biología, Universidad de Caldas, Manizales, Colombia.
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Noriega
authors.affiliation: Entomology and Nematology Department, University of Florida, Gainesville, FL, 32611, United States of America.
authors.forename: Blair
authors.initials: B
authors.lastname: Siegfried
mesh_terms: D000818:Animals; D004064:Digestive System / Q000378:metabolism*; D020869:Gene Expression Profiling; D063990:Gene Ontology; D007306:Insecticides; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D059748:Proteolysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid; D048889:Weevils / Q000201:enzymology / Q000235:genetics*; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D007306:Insecticides; D012333:RNA, Messenger; D015262:Xenobiotics; D010447:Peptide Hydrolases