title: DTDP-rhamnosyl transferase RfbF, is a newfound receptor-related regulatory protein for phage phiYe-F10 specific for Yersinia enterocolitica serotype O:3.
abstract: Bacteriophages and their hosts are continuously engaged in evolutionary competition. Here we isolated a lytic phage phiYe-F10 specific for Yersinia enterocolitica serotype O:3. We firstly described the phage receptor was regulated by DTDP-rhamnosyl transferase RfbF, encoded within the rfb cluster that was responsible for the biosynthesis of the O antigens. The deletion of DTDP-rhamnosyl transferase RfbF of wild type O:3 strain caused failure in phiYe-F10 adsorption; however, the mutation strain retained agglutination with O:3 antiserum; and complementation of its mutant converted its sensitivity to phiYe-F10. Therefore, DTDP-rhamnosyl transferase RfbF was responsible for the phage infection but did not affect recognition of Y. enterocolitica O:3 antiserum. Further, the deletions in the putative O-antigen biosynthesis protein precursor and outer membrane protein had no effect on sensitivity to phiYe-F10 infection. However, adsorption of phages onto mutant HNF10-ΔO-antigen took longer time than onto the WT, suggesting that deletion of the putative O-antigen biosynthesis protein precursor reduced the infection efficiency.
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Junrong
authors.initials: J
authors.lastname: Liang
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.|Wuhu Municipal Centre for Disease Control and Prevention, No. 178, Jiuhua central Road, Wuhu, Anhui Province, 241000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zha
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Yuhuang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Huijing
authors.initials: H
authors.lastname: Hao
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.|Department of Pathogenic Biology, School of Medical Science, Jiangsu University, Xuefu Road, Zhenjiang, Jiangsu Province, 212013, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Duan
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, No. 15, Datun Road, Chaoyang, Beijing, 100101, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Su
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, No.155, Changbai Road, Changping, Beijing, 102206, China.
authors.forename: Huaiqi
authors.initials: H
authors.lastname: Jing
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics* / Q000276:immunology; D004251:DNA Transposable Elements / Q000235:genetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism; D019081:O Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D065288:Serogroup; D013942:Thymine Nucleotides / Q000378:metabolism; D014166:Transferases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015008:Yersinia enterocolitica / Q000201:enzymology / Q000235:genetics* / Q000276:immunology
chemical_list: D004251:DNA Transposable Elements; D009702:Nucleoside Diphosphate Sugars; D019081:O Antigens; D013942:Thymine Nucleotides; C073293:thymidine diphosphate rhamnose; D014166:Transferases