title: Protective effects of Nigella sativa on gamma radiation-induced jejunal mucosal damage in rats.
abstract: BACKGROUND
  The aim of this study was to compare the efficacy of Nigella sativa in protection of jejunal mucosa against harmful effects of gamma radiation.
  
  
  METHODS
  Radiotherapy group received abdominal gamma radiation of 15Gy in addition to physiological saline. Radiotherapy+Nigella sativa treatment group received abdominal gamma radiation of 15Gy in addition to Nigella sativa treatment in the amount of 400mg/kg. Radiotherapy and treatment groups were sacrificed 3 days after the exposure to irradiation. Then, jejunum samples were harvested for biochemical and histological assessment of mucosal injury.
  
  
  RESULTS
  Nigella sativa treatment was found to significantly lower elevated tissue malondialdehyde (MDA) levels and, to raise reduced glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activity in intestinal tissues samples. Single dose 15Gy gamma-irradiation was noted to result in a marked jejunal mucosal injury. Three days after exposure to irradiation, the villi and Lieberkühn crypts were observed as denuded, and villous height diminished. Concomitantly with inflammatory cell invasion, capillary congestion and ulceration were observed in the atrophic mucosa. Nigella sativa treatment significantly attenuated the radiation induced morphological changes in the irradiated rat jejunal mucosa.
  
  
  CONCLUSIONS
  Nigella sativa has protective effects against radiation-induced damage, suggesting that clinical transfer is feasible.
authors.affiliation: Department of Anesthesiology and Reanimation, Istanbul Medeniyet University Goztepe Training and Research Hospital, Istanbul, Turkey.
authors.forename: Zeynep Nur
authors.initials: ZN
authors.lastname: Orhon
authors.affiliation: Department of Radiation Oncology, Faculty of Medicine, Trakya University, Edirne, Turkey.
authors.forename: Cem
authors.initials: C
authors.lastname: Uzal
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Istanbul Medeniyet University, Istanbul, Turkey. Electronic address: mkanter65@yahoo.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kanter
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Namık Kemal University, Tekirdağ, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Erboga
authors.affiliation: Department of Orthopaedics and Traumatology, Istanbul Medeniyet University Goztepe Training and Research Hospital, Istanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Demiroglu
mesh_terms: D000818:Animals; D005720:Gamma Rays; D005979:Glutathione Peroxidase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D031881:Nigella sativa; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Apoptosis; Jejunal mucosa; Nigella sativa; Oxidative stress; Radiation injury