title: Development of a fast DNA extraction method for sea food and marine species identification.
abstract: The authentication of food components is one of the key issues in food safety. Similarly taxonomy, population and conservation genetics as well as food web structure analysis, also rely on genetic analyses including the DNA barcoding technology. In this scenario we developed a fast DNA extraction method without any purification step from fresh and processed seafood, suitable for any PCR analysis. The protocol allows the fast DNA amplification from any sample, including fresh, stored and processed seafood and from any waste of industrial fish processing, independently of the sample storage method. Therefore, this procedure is particularly suitable for the fast processing of samples and to carry out investigations for the authentication of seafood by means of DNA analysis.
authors.affiliation: National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: marcello.tagliavia@iamc.cnr.it.
authors.forename: Marcello
authors.initials: M
authors.lastname: Tagliavia
authors.affiliation: National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: aldo.nicosia@iamc.cnr.it.
authors.forename: Aldo
authors.initials: A
authors.lastname: Nicosia
authors.affiliation: National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: monica.salamone@iamc.cnr.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Salamone
authors.affiliation: National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: girolama.biondo@iamc.cnr.it.
authors.forename: Girolama
authors.initials: G
authors.lastname: Biondo
authors.affiliation: National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: carmelo.bennici@iamc.cnr.it.
authors.forename: Carmelo Daniele
authors.initials: CD
authors.lastname: Bennici
authors.affiliation: National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: salvatore.mazzola@iamc.cnr.it.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mazzola
authors.affiliation: National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address: angela.cuttitta@iamc.cnr.it.
authors.forename: Angela
authors.initials: A
authors.lastname: Cuttitta
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D005399:Fishes / Q000145:classification* / Q000235:genetics*; D017747:Seafood / Q000032:analysis / Q000145:classification*
chemical_list: D004247:DNA
keywords: DNA barcoding; DNA extraction; Marine species; Mislabeled seafood; PCR amplification; Species identification