title: Anticoagulants used in plasma collection affect adipokine multiplexed measurements.
abstract: Obesity is an important health problem worldwide. Adipose tissue acts as an endocrine organ that secretes various bioactive substances, called adipokines, including pro-inflammatory biomarkers such as TNF-α, IL-6, leptin and C-reactive protein (CRP) and anti-inflammatory molecules such as adiponectin. The deregulated production of adipokines in obesity is linked to the pathogenesis of various disease processes and monitoring their variation is critical to understand metabolic diseases. The aim of this study was to determine the plasma concentration of adipokines in healthy subjects by multiplexed measurements and the effect of anticoagulants on their levels. Plasma samples from 10 healthy donors were collected in two different anticoagulants (sodium citrate or heparin). All markers, excluding TNF-α, showed significantly higher concentrations in heparinized compared to citrate plasma. However, levels of adipokines in different plasma samples were highly correlated for most of these markers. We reported that different anticoagulants used in the preparation of the plasma samples affected the measurements of some adipokines. The importance of the present results in epidemiology is relevant when comparing different studies in which blood samples were collected with different anticoagulants.
authors.affiliation: Human Genetics Foundation - HuGeF, Via Nizza 52, 10126 Turin, Italy; Department of Medical Sciences, University of Turin, Via Santena 19, 10100 Turin, Italy. Electronic address: alessandra.allione@hugef-torino.org.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Allione
authors.affiliation: Human Genetics Foundation - HuGeF, Via Nizza 52, 10126 Turin, Italy; Department of Medical Sciences, University of Turin, Via Santena 19, 10100 Turin, Italy.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Di Gaetano
authors.affiliation: Bioclarma Srl, Via Nizza 52, 10126 Turin, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Dani
authors.affiliation: Bioclarma Srl, Via Nizza 52, 10126 Turin, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Barberio
authors.affiliation: IRCCS Foundation, Istituto Nazionale dei Tumori, Via Venezian, 1, 20133 Milan, Italy.
authors.forename: Sabina
authors.initials: S
authors.lastname: Sieri
authors.affiliation: IRCCS Foundation, Istituto Nazionale dei Tumori, Via Venezian, 1, 20133 Milan, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Krogh
authors.affiliation: Human Genetics Foundation - HuGeF, Via Nizza 52, 10126 Turin, Italy; Department of Medical Sciences, University of Turin, Via Santena 19, 10100 Turin, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Matullo
mesh_terms: D054392:Adipokines / Q000097:blood*; D052242:Adiponectin / Q000097:blood; D000328:Adult; D000925:Anticoagulants / Q000737:chemistry*; D001800:Blood Specimen Collection; D002097:C-Reactive Protein / Q000032:analysis; D002951:Citrates; D005260:Female; D064368:Healthy Volunteers; D006493:Heparin; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D015850:Interleukin-6 / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D000077559:Sodium Citrate
chemical_list: C499918:ADIPOQ protein, human; D054392:Adipokines; D052242:Adiponectin; D000925:Anticoagulants; D002951:Citrates; C508600:IL6 protein, human; D015850:Interleukin-6; D020738:Leptin; D000077559:Sodium Citrate; D006493:Heparin; D002097:C-Reactive Protein
keywords: Adipokines; Anticoagulants; Cytokines; Microbead assay; Plasma