title: PARP6 acts as a tumor suppressor via downregulating Survivin expression in colorectal cancer.
abstract: Poly (ADP-ribose) polymerases (PARPs) are enzymes that transfer ADP-ribose groups to target proteins and are involved in a variety of biological processes. PARP6 is a novel member, and our previous findings suggest that PARP6 may act as a tumor suppressor via suppressing cell cycle progression. However, it is still unclear that PARP6 function besides growth suppression in colorectal cancer (CRC). In this study, we examined tumor suppressive roles of PAPR6 in CRC cells both in vitro and in vivo. We found that PARP6 inhibited colony formation, invasion and migration as well as cell proliferation. Moreover, ectopic overexpression of PARP6 decreased Survivin expression, which acts as an oncogene and is involved in apoptosis and mitosis. We confirmed the inverse correlation between PARP6 and Survivin expression in CRC cases by immunohistochemistry. Importantly, CRC cases with downregulation of PARP6 and upregulation of Survivin showed poor prognosis. In summary, PARP6 acts as a tumor suppressor via downregulating Survivin expression in CRC. PARP6 can be a novel diagnostic and therapeutic target together with Survivin for CRC.
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.|Department of Health Sciences, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Guangying
authors.initials: G
authors.lastname: Qi
authors.affiliation: Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Japan.
authors.forename: Yasusei
authors.initials: Y
authors.lastname: Kudo
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541000, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Tian
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Shengjian
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Mi
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Shao
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tsunematsu
authors.affiliation: Department of Oral Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Japan.
authors.forename: Naozumi
authors.initials: N
authors.lastname: Ishimaru
authors.affiliation: Department of Pathology and Physiopathology, Guilin Medical University, Guilin 541004, People's Republic of China.
authors.forename: Sien
authors.initials: S
authors.lastname: Zeng
authors.affiliation: Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Hiroshima 727-0023, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tatsuka
authors.affiliation: Department of Health Sciences, Prefectural University of Hiroshima, Hiroshima 734-8558, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Shimamoto
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008297:Male; D015398:Signal Transduction; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; D036002:ADP Ribose Transferases; C579799:PARP6 protein, human
keywords: PARP6; Survivin; colorectal cancer; prognosis; tumor suppressor