title: [Automatically feeding strategy for 1,3-propanediol fermentation of Klebsiella pneumoniae LDH526].
abstract: 1,3-propanediol is an important chemical used as building block for the synthesis of highly promising polyesters such as polytrimethylene terephthalate. A genetically modified Klebsiella pneumoniae LDH526 can use glycerol as sole carbon source and produce 1,3-propanediol with the titer above 90 g/L. A key factor affecting the production of 1,3-propanediol by the mutant K. pneumoniae is the accurate control of the feeding of glycerol. To generate a robust and reproducible fermentation process of 1,3-propanediol, we designed and optimized an automatically feeding strategy of glycerol based on fermentation kinetics. By coupling the substrate feeding rate with easily observed variables -pH and fermentation time, we have achieved self-starting glycerol feeding and dynamic control of the glycerol concentration during the fermentation process. This automated system allowed us to generate a reproducible, consistent and operator-independent process from lab-scale to production scale. The final concentration of 1,3-propanediol was above 95 g/L after 72 h.
authors.forename: Jinhai
authors.initials: J
authors.lastname: Huang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Dehua
authors.initials: D
authors.lastname: Liu
mesh_terms: D003470:Culture Media; D005285:Fermentation; D005990:Glycerol; D007218:Industrial Microbiology / Q000379:methods*; D007711:Klebsiella pneumoniae / Q000254:growth & development*; D019946:Propylene Glycol; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D019946:Propylene Glycol; D005990:Glycerol