title: Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens.
abstract: As an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. ProGastro SSC and Shiga toxin-producing Escherichia coli (i.e., STEC) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. Following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column methods. Mean processing times for manual and column methods were 30.5 and 69.2 min, respectively. Concordance of investigational methods with automated extraction was ≥98.8%.
authors.affiliation: College of Health Sciences, Marquette University, Milwaukee, Wisconsin, USA erik.munson@marquette.edu.
authors.forename: Erik
authors.initials: E
authors.lastname: Munson
authors.affiliation: Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA.
authors.forename: Maureen
authors.initials: M
authors.lastname: Napierala
authors.affiliation: Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA.
authors.forename: Kimber L
authors.initials: KL
authors.lastname: Munson
authors.affiliation: Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA.
authors.forename: Dorothy
authors.initials: D
authors.lastname: Bilbo
authors.affiliation: Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Schulte
mesh_terms: D001424:Bacterial Infections / Q000175:diagnosis*; D005243:Feces / Q000382:microbiology*; D005759:Gastroenteritis / Q000175:diagnosis*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009696:Nucleic Acids / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D009696:Nucleic Acids