title: [Relationship between interferon-α conformation and its anti-viral activity determined by circular dichroism and flow cytometry].
abstract: The relationship between the conformation of interferon-α (IFN-α) and its anti-viral activity were analyzed by circular dichroism (CD) and flow cytometry (FCM) techniques. The recombinant human IFN-α (rIFN-α2b and rIFN-α2a) were used. CD spectra from 190 nm to 240 nm indicated that two the IFN-α showed stable secondary structure at 65 degrees C, but unstable when the temperature was above 65 degrees C, and the change was irreversible. FCM data of the anti-viral activity of IFN-α indicated that the change of its secondary structures partly weakened its anti-viral activity. The rIFN-α2b and rIFN-α2a showed the same phenomenon. These data indicated that the conformation of IFN-α is one of the factors to influence its anti-viral activity and the combination of CD and FCM is a good method to analyze the relationship between the conformation of protein drugs and their biological activities in single cell level.
authors.forename: Maozhi
authors.initials: M
authors.lastname: Hu
authors.forename: Wenhua
authors.initials: W
authors.lastname: Li
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Yan
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.forename: Guiyou
authors.initials: G
authors.lastname: Cui
authors.forename: Xin'an
authors.initials: X
authors.lastname: Jiao
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry*; D002942:Circular Dichroism; D005434:Flow Cytometry; D006801:Humans; D016898:Interferon-alpha / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D011994:Recombinant Proteins