title: Characterization of pollen and bacterial community composition in brood provisions of a small carpenter bee.
abstract: Many insects obtain gut microbes from their diet, but how a mother's foraging patterns influence the microbes found in her offspring's food remains an open question. To address this gap, we studied a bee that forages for pollen from multiple species of plants and may therefore acquire diverse bacteria from different plants. We tested the hypothesis that pollen diversity correlates with bacterial diversity by simultaneously characterizing these two communities in bee brood provisions for the first time. We used deep sequencing of the plant RBCL gene and the bacterial 16S rRNA gene to characterize pollen and bacterial diversity. We then tested for associations between pollen and bacterial species richness and community composition, as well as co-occurrence of specific bacteria and pollen types. We found that both pollen and bacterial communities were extremely diverse, indicating that mother bees visit a wide variety of flowers for pollen and nectar and subsequently bring a diversity of microbes back into their nests. Pollen and bacterial species richness and community composition, however, were not correlated. Certain pollen types significantly co-occurred with the most proportionally abundant bacteria, indicating that the plants these pollen types came from may serve as reservoirs for these bacteria. Even so, the overall diversity of these communities appears to mask these associations at a broader scale. Further study of these pollen and bacteria associations will be important for understanding the complicated relationship between bacteria and wild bees.
authors.affiliation: Department of Entomology, University of California, Riverside, 900 University Avenue, Riverside, CA, 92521, USA.
authors.forename: Quinn S
authors.initials: QS
authors.lastname: McFrederick
authors.affiliation: Department of Biological Sciences, University of New Hampshire, 46 College Road, Durham, NH, 03824, USA.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Rehan
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification*; D001516:Bees / Q000382:microbiology*; D058893:DNA Barcoding, Taxonomic; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005260:Female; D035264:Flowers; D005798:Genes, Bacterial; D057048:Plant Nectar; D011058:Pollen / Q000145:classification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; D057048:Plant Nectar; D012336:RNA, Ribosomal, 16S
keywords: Ceratina calcarata; Lactobacillus; Polylectic pollen forager; microbiome; palynology; pollination ecology