title: [STUDY OF PROTECTIVE ACTIVITY OF PROTEIN-CONTAINING ANTIGENS OF STREPTOCOCCUS PNEUMONIAE IN A HETEROLOGOUS SYSTEM].
abstract: OBJECTIVE
  Study protective activity of protein-containing antigens of pneumococcus, obtained from serotypes 6B, 10A, 14, 19F, 23F and 36R, against infection with heterologous strains of S. pneumoniae.
  
  
  METHODS
  S. pneumoniae strains of serotypes 3, 6B, 10A, 14, 19F, 23F and 36R, obtained from the collection of pneumococcus strains of Mechnikov RIVS, were used in the study. Protein-containing antigens of S. pneumoniae were isolated by acetone precipitations of supernatant fraction of culture medium. Protective activity of preparations of protein-containing antigens of pneumococcus as studied in experiments of active protection of BALb/c line mice.
  
  
  RESULTS
  The data obtained give evidence, that protein-containing antigens of pneumococcus, isolated from serotypes 6B, 10A, 14, 19F and 23F, effectively protect animals from subsequent infection with a heterologous S. pneumoniae strain of serotype 3 No. 11/56. Protection was noted at a level from 80 to 100% (p ≤ 0.05). Similar protective effect was detected in another experiment in a group of mice, immunized with preparations of protein-containing antigens of pneumococcus, obtained from serotypes 6B and 36R, against infection with a heterologous S. pneumoniae strain of serotype 3 No. 11/56. Protection was noted at a level of 90% (p ≤ 0.05).
  
  
  CONCLUSIONS
  The results of the experiments carried out allow to assume, that the main role in formation of cross-protection in experiments in animals is played by pneumococcus, proteins, that are a part of the studied preparations, and not polysaccharide antigens.
authors.forename: D S
authors.initials: DS
authors.lastname: Vorobiev
authors.forename: I B
authors.initials: IB
authors.lastname: Semenova
authors.forename: Yu V
authors.initials: YV
authors.lastname: Volokh
authors.forename: E E
authors.initials: EE
authors.lastname: Romanenko
authors.forename: A P
authors.initials: AP
authors.lastname: Baturo
authors.forename: N A
authors.initials: NA
authors.lastname: Mikhailova
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D056738:Cross Protection; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D011018:Pneumonia, Pneumococcal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D065288:Serogroup; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000276:immunology*; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D022242:Pneumococcal Vaccines