title: The implications of endemic IMP-4 carbapenemase for clinical laboratory susceptibility testing.
abstract: A local predominance of carbapenemase producing Enterobacteriaceae with low minimum inhibitory concentrations (MIC) to meropenem prompted a review of methods available for carbapenemase detection. We report on results using two selective media, temocillin discs, CarbaNP test, GeneXpert Carba-R assay and an in-house PCR assay.
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia.
authors.forename: Namraj
authors.initials: N
authors.lastname: Goire
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia.
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Harnett
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia. Electronic address: Lyn.O'Reilly@health.wa.gov.au.
authors.forename: Lyn C
authors.initials: LC
authors.lastname: O'Reilly
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Perth, Western Australia 6150, Australia; School of Pathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia 6009, Australia.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Ingram
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia; School of Pathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia 6009, Australia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Leung
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia; School of Medicine and Pharmacology, University of Western Australia, Crawley, Western Australia 6009, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Speers
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Healy
authors.affiliation: Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia; School of Pathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia 6009, Australia.
authors.forename: Timothy J J
authors.initials: TJ
authors.lastname: Inglis
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D013845:Thienamycins; C425142:beta-lactamase IMP-4; D001618:beta-Lactamases; D000077731:Meropenem
keywords: CarbaNP test; Carbapenemase producing Enterobacteriaceae; GeneXpert Carba-R assay