title: HLA-G expression and regulation during Pseudomonas aeruginosa infection in cystic fibrosis patients.
abstract: BACKGROUND
  Deregulated immune response fails to control biofilm-forming bacteria, as Pseudomonas aeruginosa, in the lungs of cystic fibrosis (CF) patients. HLA-G is an immune-modulatory molecule involved in respiratory diseases and infections.
  
  
  METHODS
  HLA-G mRNA and protein were analyzed in plasma and exhaled breath condensate from CF patients undergoing intravenous antibiotic treatment, CF cell line and murine model.
  
  
  RESULTS
  Therapy normalizes HLA-G plasmatic in CF patients suggesting a systemic anti-inflammatory role while in CF airway system, higher expression of HLA-G is associated with P. aeruginosa infection. CF cell line and murine model expressed higher HLA-G molecules in the presence of P. aeruginosa.
  
  
  CONCLUSIONS
  Plasmatic and lung HLA-G expression suggest a role in reducing systemic inflammation and supporting P. aeruginosa infection.
authors.affiliation: Section of Microbiology & Medical Genetics, Medical Sciences Department, Ferrara University, 44121 Ferrara FE, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Rizzo
authors.affiliation: Section of Microbiology & Medical Genetics, Medical Sciences Department, Ferrara University, 44121 Ferrara FE, Italy.|Department of Medicine, Cystic Fibrosis Translational Research Laboratory "Daniele Lissandrini," University of Verona, Verona, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Bergamini
authors.affiliation: Section of Microbiology & Medical Genetics, Medical Sciences Department, Ferrara University, 44121 Ferrara FE, Italy.
authors.forename: Daria
authors.initials: D
authors.lastname: Bortolotti
authors.affiliation: Louvain Centre for Toxicology & Applied Pharmacology, Université Catholique de Louvain, Place de l'Université 1, 1348, Belgium.
authors.forename: Teresinha
authors.initials: T
authors.lastname: Leal
authors.affiliation: Cystic Fibrosis Center, Azienda Ospedaliera Universitaria Integrata di Verona, 37122 Verona VR, Italy.
authors.forename: Ciro
authors.initials: C
authors.lastname: D'Orazio
authors.affiliation: Cystic Fibrosis Center, Azienda Ospedaliera Universitaria Integrata di Verona, 37122 Verona VR, Italy.
authors.forename: Emily
authors.initials: E
authors.lastname: Pintani
authors.affiliation: Section of Microbiology & Medical Genetics, Medical Sciences Department, Ferrara University, 44121 Ferrara FE, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Melchiorri
authors.affiliation: Service Planning and Management Control, University Hospital of Ferrara, Ferrara, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Zavatti
authors.affiliation: Cystic Fibrosis Center, Azienda Ospedaliera Universitaria Integrata di Verona, 37122 Verona VR, Italy.
authors.forename: Baroukh M
authors.initials: BM
authors.lastname: Assael
authors.affiliation: Department of Medicine, Cystic Fibrosis Translational Research Laboratory "Daniele Lissandrini," University of Verona, Verona, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Sorio
authors.affiliation: Cystic Fibrosis Center, Azienda Ospedaliera Universitaria Integrata di Verona, 37122 Verona VR, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Melotti
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D001980:Bronchi / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002460:Cell Line; D003550:Cystic Fibrosis / Q000150:complications* / Q000235:genetics* / Q000382:microbiology; D004195:Disease Models, Animal; D059951:HLA-G Antigens / Q000032:analysis / Q000097:blood / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D054884:Host-Pathogen Interactions; D006801:Humans; D007249:Inflammation; D008168:Lung / Q000276:immunology; D051379:Mice; D011446:Prospective Studies; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D020545:Respiratory Mucosa / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; C032907:Q surface antigens
keywords: HLA-G; Pseudomonas aeruginosa; cystic fibrosis