title: An Efficient Lanthanide-Dependent DNAzyme Cleaving 2'-5'-Linked RNA.
abstract: RNA can form two types of linkage. In addition to the predominant 3'-5' linkage, 2'-5'-linked RNA is also important in biology, medicine, and prebiotic studies. Here, in vitro selection was used to isolate a DNAzyme that specifically cleaves 2'-5' RNA by using Ce(3+) as the metal cofactor, but leaves the 3'-5' counterpart intact. This Ce5 DNAzyme requires trivalent light lanthanide ions and shows a rate of 0.16 min(-1) in the presence of 10 μm Ce(3+) ; the activity decreases with heavier lanthanide ions. This is the fastest DNAzyme reported for this reaction, and it might enable applications in chemical biology. As a proof-of-concept, using this DNAzyme, the reactions between phosphorothioate-modified RNA and strongly thiophilic metals (Hg(2+) and Tl(3+) ) were studied as a function of pH.
authors.affiliation: School of Pharmaceutical Sciences, Central South University, Changsha, Hunan, 410013, China.|Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Wenhu
authors.initials: W
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences, Central South University, Changsha, Hunan, 410013, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ding
authors.affiliation: School of Pharmaceutical Sciences, Central South University, Changsha, Hunan, 410013, China. liujw@uwaterloo.ca.|Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada. liujw@uwaterloo.ca.
authors.forename: Juewen
authors.initials: J
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D002563:Cerium / Q000737:chemistry*; D021881:DNA, Catalytic / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D008628:Mercury / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012313:RNA / Q000378:metabolism*; D059367:RNA Cleavage; D013793:Thallium / Q000737:chemistry
chemical_list: D021881:DNA, Catalytic; D007477:Ions; D002563:Cerium; D012313:RNA; D013793:Thallium; D008628:Mercury
keywords: DNAzymes; RNA; biosensors; lanthanides; phosphorothioate