title: High-Density Lipoprotein-Associated miR-223 Is Altered after Diet-Induced Weight Loss in Overweight and Obese Males.
abstract: OBJECTIVE
  microRNAs (miRNAs) are small, endogenous non-coding RNAs that regulate metabolic processes, including obesity. The levels of circulating miRNAs are affected by metabolic changes in obesity, as well as in diet-induced weight loss. Circulating miRNAs are transported by high-density lipoproteins (HDL) but the regulation of HDL-associated miRNAs after diet-induced weight loss has not been studied. We aim to determine if HDL-associated miR-16, miR-17, miR-126, miR-222 and miR-223 levels are altered by diet-induced weight loss in overweight and obese males.
  
  
  METHODS
  HDL were isolated from 47 subjects following 12 weeks weight loss comparing a high protein diet (HP, 30% of energy) with a normal protein diet (NP, 20% of energy). HDL-associated miRNAs (miR-16, miR-17, miR-126, miR-222 and miR-223) at baseline and after 12 weeks of weight loss were quantified by TaqMan miRNA assays. HDL particle sizes were determined by non-denaturing polyacrylamide gradient gel electrophoresis. Serum concentrations of human HDL constituents were measured immunoturbidometrically or enzymatically.
  
  
  RESULTS
  miR-16, miR-17, miR-126, miR-222 and miR-223 were present on HDL from overweight and obese subjects at baseline and after 12 weeks of the HP and NP weight loss diets. The HP diet induced a significant decrease in HDL-associated miR-223 levels (p = 0.015), which positively correlated with changes in body weight (r = 0.488, p = 0.032). Changes in miR-223 levels were not associated to changes in HDL composition or size.
  
  
  CONCLUSIONS
  HDL-associated miR-223 levels are significantly decreased after HP diet-induced weight loss in overweight and obese males. This is the first study reporting changes in HDL-associated miRNA levels with diet-induced weight loss.
authors.affiliation: Lipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
authors.forename: Fatiha
authors.initials: F
authors.lastname: Tabet
authors.affiliation: Lipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
authors.forename: Luisa F
authors.initials: LF
authors.lastname: Cuesta Torres
authors.affiliation: Lipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
authors.forename: Kwok Leung
authors.initials: KL
authors.lastname: Ong
authors.affiliation: Lipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
authors.forename: Sudichhya
authors.initials: S
authors.lastname: Shrestha
authors.affiliation: Lipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
authors.forename: Sébastien A
authors.initials: SA
authors.lastname: Choteau
authors.affiliation: Lipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Barter
authors.affiliation: School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, South Australia, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Clifton
authors.affiliation: Lipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
authors.forename: Kerry-Anne
authors.initials: KA
authors.lastname: Rye
mesh_terms: D000328:Adult; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000178:diet therapy*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D015431:Weight Loss
chemical_list: D008075:Lipoproteins, HDL; D035683:MicroRNAs; C525067:MIR223, human