title: Site-specific conjugation of single domain antibodies to liposomes enhances photosensitizer uptake and photodynamic therapy efficacy.
abstract: Photodynamic therapy for therapy-resistant cancers will greatly benefit from targeted delivery of tumor photosensitizing agents. In this study, a strategy for the site-specific conjugation of single domain antibodies onto liposomes containing the photosensitizer zinc phthalocyanine was developed and tested.
authors.affiliation: Department of Experimental Surgery, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. m.heger@amc.uva.nl.
authors.forename: M
authors.initials: M
authors.lastname: Broekgaarden
authors.affiliation: Department of Membrane Biochemistry and Biophysics, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: van Vught
authors.affiliation: Division of Cell Biology, Science Faculty, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Oliveira
authors.affiliation: Division of Cell Biology, Science Faculty, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: R C
authors.initials: RC
authors.lastname: Roovers
authors.affiliation: Division of Cell Biology, Science Faculty, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: P M P
authors.initials: PM
authors.lastname: van Bergen en Henegouwen
authors.affiliation: Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, NL-3508 TB, Utrecht, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Pieters
authors.affiliation: Department of Experimental Surgery, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. m.heger@amc.uva.nl.
authors.forename: T M
authors.initials: TM
authors.lastname: Van Gulik
authors.affiliation: Department of Membrane Biochemistry and Biophysics, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Breukink
authors.affiliation: Department of Experimental Surgery, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. m.heger@amc.uva.nl.
authors.forename: M
authors.initials: M
authors.lastname: Heger
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers; D006801:Humans; D007211:Indoles / Q000737:chemistry; D054833:Isoindoles; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D050997:Nanomedicine / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D061905:Single-Domain Antibodies / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry; D017967:Zinc Compounds
chemical_list: D004337:Drug Carriers; D007211:Indoles; D054833:Isoindoles; D008081:Liposomes; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D061905:Single-Domain Antibodies; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D015032:Zinc; D010100:Oxygen