title: Biosynthetic Pathway of the Reduced Polyketide Product Citreoviridin in Aspergillus terreus var. aureus Revealed by Heterologous Expression in Aspergillus nidulans.
abstract: Citreoviridin (1) belongs to a class of F1-ATPase β-subunit inhibitors that are synthesized by highly reducing polyketide synthases. These potent mycotoxins share an α-pyrone polyene structure, and they include aurovertin, verrucosidin, and asteltoxin. The identification of the citreoviridin biosynthetic gene cluster in Aspergillus terreus var. aureus and its reconstitution using heterologous expression in Aspergillus nidulans are reported. Two intermediates were isolated that allowed the proposal of the biosynthetic pathway of citreoviridin.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , 1985 Zonal Avenue, Los Angeles, California 90089, United States.
authors.forename: Tzu-Shyang
authors.initials: TS
authors.lastname: Lin
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , 1985 Zonal Avenue, Los Angeles, California 90089, United States.|Department of Pharmacy, Chia Nan University of Pharmacy and Science , Tainan 71710, Taiwan.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chiang
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , 1985 Zonal Avenue, Los Angeles, California 90089, United States.|Department of Chemistry, University of Southern California, College of Letters, Arts, and Sciences , Los Angeles, California 90089, United States.
authors.forename: Clay C C
authors.initials: CC
authors.lastname: Wang
mesh_terms: D001233:Aspergillus nidulans / Q000737:chemistry* / Q000235:genetics; D001313:Aurovertins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D053898:Biosynthetic Pathways; D015394:Molecular Structure; D005810:Multigene Family; D009183:Mycotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D048630:Polyketide Synthases / Q000378:metabolism*; D061065:Polyketides / Q000378:metabolism; D011753:Pyrones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D001313:Aurovertins; D009183:Mycotoxins; D061065:Polyketides; D011753:Pyrones; D048630:Polyketide Synthases; C048224:verrucosidin; C014416:citreoviridin