title: Dendrobium moniliforme Exerts Inhibitory Effects on Both Receptor Activator of Nuclear Factor Kappa-B Ligand-Mediated Osteoclast Differentiation in Vitro and Lipopolysaccharide-Induced Bone Erosion in Vivo.
abstract: Dendrobium moniliforme (DM) is a well-known plant-derived extract that is widely used in Oriental medicine. DM and its chemical constituents have been reported to have a variety of pharmacological effects, including anti-oxidative, anti-inflammatory, and anti-tumor activities; however, no reports discuss the beneficial effects of DM on bone diseases such as osteoporosis. Thus, we investigated the relationship between DM and osteoclasts, cells that function in bone resorption. We found that DM significantly reduced receptor activator of nuclear factor kappa-B ligand (RANKL)-induced tartrate-resistant acid phosphatase (TRAP)-positive osteoclast formation; DM directly induced the down-regulation of c-Fos and nuclear factor of activated T cells c1 (NFATc1) without affecting other RANKL-dependent transduction pathways. In the later stages of osteoclast maturation, DM negatively regulated the organization of filamentous actin (F-actin), resulting in impaired bone-resorbing activity by the mature osteoclasts. In addition, micro-computed tomography (μ-CT) analysis of the murine model revealed that DM had a beneficial effect on lipopolysaccharide (LPS)-mediated bone erosion. Histological analysis showed that DM attenuated the degradation of trabecular bone matrix and formation of TRAP-positive osteoclasts in bone tissues. These results suggest that DM is a potential candidate for the treatment of metabolic bone disorders such as osteoporosis.
authors.affiliation: Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. phone8418@hanmail.net.
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Baek
authors.affiliation: Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. Kimjy1014@gmail.com.|Institute for Skeletal Disease, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. Kimjy1014@gmail.com.|Institute for Environmental Science, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. Kimjy1014@gmail.com.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. asj0427@naver.com.
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Ahn
authors.affiliation: Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. hanleuni@naver.com.
authors.forename: Yoon-Hee
authors.initials: YH
authors.lastname: Cheon
authors.affiliation: Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. yangm@wku.ac.kr.|Institute for Environmental Science, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. yangm@wku.ac.kr.
authors.forename: Miyoung
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. jmoh@wku.ac.kr.|Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. jmoh@wku.ac.kr.|Institute for Skeletal Disease, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. jmoh@wku.ac.kr.
authors.forename: Jaemin
authors.initials: J
authors.lastname: Oh
authors.affiliation: Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. mkchoi@wku.ac.kr.|Institute for Environmental Science, Wonkwang University, Iksan, Jeonbuk 570-749, Korea. mkchoi@wku.ac.kr.
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Choi
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D031667:Dendrobium / Q000737:chemistry*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000009:adverse effects; D051379:Mice; D050778:NFATC Transcription Factors / Q000378:metabolism*; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology; D053245:RANK Ligand / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D050778:NFATC Transcription Factors; C494005:Nfatc1 protein, mouse; D010936:Plant Extracts; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse
keywords: Dendrobium moniliforme (DM); bone resorption; osteoclast; osteoporosis