title: Bisphenol A exposure and healing effects of Adiantum capillus-veneris L. plant extract (APE) in bisphenol A-induced reproductive toxicity in albino rats.
abstract: The current study presents the bisphenol A exposure and the ameliorative effects of Adiantum capillus-veneris on testicular toxicity induced by bisphenol A. Adult male albino rats were divided into five groups of five animals each: A (control), B (vehicle control), C (toxic), D (protective), and E (ameliorative) were served distilled water, olive oil, bisphenol A (BPA) at 100 mg/kg body weight, A. capillus-veneris plant extract at 25 mg/kg body weight, and BPA + A. capillus-veneris, respectively. All of the doses were administered orally for 15 days, and the rats were then sacrificed. Blood samples for the testosterone assay and both testes were collected for histological examination. The body weight, paired testes weight, relative tissue weight index, Johnsen scoring of tubules, and level of serum testosterone decreased in BPA-treated rats. Similarly, histological examination of the testes in BPA-treated animals revealed a lower number of Leydig cells, an irregular basement membrane, sloughing of germinal layers, vacuolization, a lower number of spermatocytes, and debris in the lumen. However, co-administration of A. capillus-veneris with BPA increased the total antioxidative capacity (330.82 ± 22.46 μmol/mg protein) of the testes and restored the serum testosterone level (1.70 ng/ml); histological features showed restoration in the stages of spermatogenesis. Conclusively, A. capillus-veneris plant extract overcomes the estrogenic effects of BPA on the reproductive system of rats and protects rats' testes against BPA-induced injury/damage via an antioxidative mechanism that appears to be conciliated.
authors.affiliation: Chinese Academy of Science (CAS) Key Laboratory of Crust-Mantle Materials and the Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei, 230026, People's Republic of China.
authors.forename: Balal
authors.identifier: http://orcid.org/0000-0003-2732-2176
authors.initials: B
authors.lastname: Yousaf
authors.affiliation: School of Resource and Environment, Anhui Agriculture University, Hefei, 230026, People's Republic of China.|Environmental Toxicology Lab, College of Earth and Environmental Science, University of the Punjab, Lahore, 54590, Pakistan.
authors.lastname: Amina
authors.affiliation: Chinese Academy of Science (CAS) Key Laboratory of Crust-Mantle Materials and the Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei, 230026, People's Republic of China. lgj@ustc.edu.cn.
authors.forename: Guijian
authors.initials: G
authors.lastname: Liu
authors.affiliation: Chinese Academy of Science (CAS) Key Laboratory of Crust-Mantle Materials and the Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei, 230026, People's Republic of China.
authors.forename: Ruwei
authors.initials: R
authors.lastname: Wang
authors.affiliation: Environmental Toxicology Lab, College of Earth and Environmental Science, University of the Punjab, Lahore, 54590, Pakistan.
authors.forename: Abdul
authors.initials: A
authors.lastname: Qadir
authors.affiliation: Chinese Academy of Science (CAS) Key Laboratory of Crust-Mantle Materials and the Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei, 230026, People's Republic of China.
authors.forename: Muhammad Ubaid
authors.initials: MU
authors.lastname: Ali
authors.affiliation: Environmental Toxicology Lab, College of Earth and Environmental Science, University of the Punjab, Lahore, 54590, Pakistan.
authors.forename: Qudsia
authors.initials: Q
authors.lastname: Kanwal
authors.affiliation: Environmental Toxicology Lab, College of Earth and Environmental Science, University of the Punjab, Lahore, 54590, Pakistan.
authors.forename: Bushra
authors.initials: B
authors.lastname: Munir
authors.affiliation: Developmental Biology Lab, Department of Zoology, University of the Punjab, Lahore, 54590, Pakistan.
authors.lastname: Asmatullah
authors.affiliation: Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, 54590, Pakistan.
authors.forename: Zaigham
authors.initials: Z
authors.lastname: Abbas
mesh_terms: D035681:Adiantum; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001559:Benzhydryl Compounds / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D012098:Reproduction / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013739:Testosterone / Q000097:blood; D000098826:Bisphenol A Compounds
chemical_list: D000975:Antioxidants; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D010936:Plant Extracts; D013739:Testosterone; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: A. capillus-veneris; Bisphenol A; Exposure; Rats; Rehabilitation; Testicular injury