title: Targeting blockage of STAT3 inhibits hepatitis B virus-related hepatocellular carcinoma.
abstract: Hepatitis B virus (HBV) infection is a significant cause of liver disease pathogenesis, which results in the development of hepatic dysfunction, cirrhosis and hepatocellular carcinoma (HCC). Our previous studies showed that oncogene STAT3 might be an ideal target for HCC therapy. Here, we investigated whether targeting blockage of STAT3 signaling is efficient for HBV-related HCC. Based on the refractory of HCC and the persistence of HBV, in this study, we designed shRNAs targeting STAT3. The results showed that blocking STAT3 signaling by shRNAs could promote HBV positive HCC cell apoptosis and induce cell cycle arrest, resulting in HCC cell growth inhibition in vitro. Importantly, STAT3-shRNAs efficiently suppressed HBV replication, which would reduce HBV-derived stimulation to STAT3 signaling and augment STAT3-shRNAs-mediated anti-HCC effect. Finally, STAT3-shRNAs-mediated anti-HBV positive HCC effect was confirmed in xenograft nude mice. This study suggested that targeting STAT3 therapies such as STAT3-shRNAs may be an efficacious strategy for HBV-related HCC.
authors.affiliation: a Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University , China.
authors.forename: Yinli
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University , China.
authors.forename: Bingqing
authors.initials: B
authors.lastname: Zheng
authors.affiliation: a Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University , China.
authors.forename: Qiuju
authors.initials: Q
authors.lastname: Han
authors.affiliation: a Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University , China.
authors.forename: Cai
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University , China.|b School of Life Sciences, University of Science and Technology of China , China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Tian
authors.affiliation: a Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University , China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D014162:Transfection
chemical_list: D050796:STAT3 Transcription Factor
keywords: Gene targeted therapy; HBV; HCC; STAT3