title: Pubertal exposure to ethinylestradiol promotes different effects on the morphology of the prostate of the male and female gerbil during aging.
abstract: In rodents, the final growth and maturation of the prostate occur at puberty, a crucial period for prostate development. The present study is a serological, morphological, morphometric, and immunohistochemical analysis of the effects of exposure to ethinylestradiol (EE) (15 µg/kg/day) during puberty (EE/PUB group) on the male ventral and female prostate in senile gerbils. In the study, male and female gerbils (Meriones unguiculatus) (42 days) received by gavage 15 μg/kg/day of EE (a component of the contraceptive pill), diluted in 100 µL of Nujol® for 1 week (EE/PUB group). In the control group, males and females were not treated. Animals were killed (n = 5) after 12 months in the experimental groups. In the senile male in the EE/PUB group, we observed a reduction in testosterone levels and a decrease in the prostatic epithelial thickness, as well as in the thickness of the muscle layer. In addition, an increase in PIN multiplicity and prostatic inflammation was observed. In the senile female in the EE/PUB group, we observed increased testosterone and estradiol levels, an enhanced prostatic epithelial thickness and an increase in the thickness of the muscle layer. Immunohistochemical analysis revealed an increase in positive cells (%) for AR and PCNA in the male prostate and an increase in positive basal cells for p63 in the female prostate of the EE/PUB group. Exposure to EE during puberty resulted in an inhibitory action on the male ventral prostate and an anabolic effect on the female prostate in senile gerbils. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 477-489, 2017.
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, UNICAMP, CP 6109, Campinas, São Paulo, 13084-864, Brazil.|Special Institute of Health Sciences, Medicine Course, Federal University of Goiás, Jataí, Goiás, 75804-020.
authors.forename: Ana P S
authors.initials: AP
authors.lastname: Perez
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, UNICAMP, CP 6109, Campinas, São Paulo, 13084-864, Brazil.
authors.forename: Manoel F
authors.initials: MF
authors.lastname: Biancardi
authors.affiliation: Department of Biology, Laboratory of Microscopy and Microanalysis, IBILCE, University of Estadual Paulista-UNESP, São José Do Rio Preto, São Paulo, 15054-000, Brazil.
authors.forename: Cássia R S
authors.initials: CR
authors.lastname: Caires
authors.affiliation: Department of Biology, Laboratory of Microscopy and Microanalysis, IBILCE, University of Estadual Paulista-UNESP, São José Do Rio Preto, São Paulo, 15054-000, Brazil.
authors.forename: Luiz R
authors.initials: LR
authors.lastname: Falleiros
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, UNICAMP, CP 6109, Campinas, São Paulo, 13084-864, Brazil.|Department of Biology, Laboratory of Microscopy and Microanalysis, IBILCE, University of Estadual Paulista-UNESP, São José Do Rio Preto, São Paulo, 15054-000, Brazil.
authors.forename: Rejane M
authors.initials: RM
authors.lastname: Góes
authors.affiliation: Department of Morphology, Federal University of Goiás, Goiânia, Goiás, 74001-970, Brazil.
authors.forename: Fernanda C A
authors.initials: FC
authors.lastname: Santos
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, UNICAMP, CP 6109, Campinas, São Paulo, 13084-864, Brazil.|Department of Biology, Laboratory of Microscopy and Microanalysis, IBILCE, University of Estadual Paulista-UNESP, São José Do Rio Preto, São Paulo, 15054-000, Brazil.
authors.forename: Sebastião R
authors.initials: SR
authors.lastname: Taboga
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000097:blood; D047628:Estrogen Receptor alpha / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000633:toxicity*; D005260:Female; D005849:Gerbillinae; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D015534:Trans-Activators / Q000378:metabolism; D014746:Vimentin
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D018809:Proliferating Cell Nuclear Antigen; D015534:Trans-Activators; D014746:Vimentin; D013739:Testosterone; D004997:Ethinyl Estradiol; D004958:Estradiol
keywords: ethinylestradiol; female; male; prostate; puberty; senescence