title: Quantification of Quercetin and Rutin from Benincasa hispida Seeds and Carissa Congesta Roots by High-performance Thin Layer Chromatography and High-performance Liquid Chromatography.
abstract: OBJECTIVE
  In Indian Ayurvedic system, Benincasa hispida (BH) and Carissa congesta (CC) are well-known plants used for major and minor ailments. BH has been regarded as Kushmanda, whereas CC has been used in immune-related disorders of the human system. Quercetin and rutin identified from the vast plethora of plant extracts have proved to possess ethnopharmacological relevance.
  
  
  METHODS
  In present studies, we have determined quercetin and rutin in terms of percentage in BH seeds and CC roots by high-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC). After extraction and phytochemical screening, the extracts were subjected to quantification for the presence of quercetin and rutin by HPTLC and HPLC.
  
  
  RESULTS
  HPTLC showed quercetin as 44.60, 27.13% and rutin as 32.00, 36.31% w/w, whereas HPLC revealed quercetin as 34.00, 35.00% and rutin as 21.99, 45.03% w/v in BH and CC extracts, respectively.
  
  
  CONCLUSIONS
  The BH and CC extracts have elucidated peaks that were corresponding with standard peaks on undertaking chromatographic studies.
  
  
  CONCLUSIONS
  Quercetin and rutin are isolated from BH seeds and CC roots by High Performance. Thin Layer Chromatography and High Performance Liquid Chromatography. HPTLC revealed presence of quercetin as 44.60, 27.13 % and rutin as 32.00, 36.31 % w/w. HPLC revealed presence of quercetin as 34.00, 35.00 % and rutin as 21.99, 45.03 % w/v. Abbreviation Used: HPTLC: High Performance Thin Layer Chromatography; HPLC: High Pressure Liquid Chromatography, UV: Ultraviolet, CC: Carissa congesta, BH: Benincasa hispida.
authors.affiliation: Department of Pharmacology, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India; Ph.D. Research Scholar, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India.
authors.forename: Gaurav Mahesh
authors.initials: GM
authors.lastname: Doshi
authors.affiliation: Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, Maharashtra, India.
authors.forename: Hemant Devidas
authors.initials: HD
authors.lastname: Une
keywords: Benincasa hispida; Carissa congesta; high-performance liquid chromatography; high-performance thin layer chromatography; quercetin; rutin