title: Determining the roles of a conserved tyrosine residue in a Mip-like peptidyl-prolyl cis-trans isomerase.
abstract: The FKBP22 and the related peptidyl-prolyl cis-trans isomerases dimerize using their N-terminal domains. Conversely, their C-terminal domains possess both the substrate and inhibitor binding sites. To delineate the roles of a conserved Tyr residue at their N-terminal domains, we have studied a FKBP22 mutant that carries an Ala in place of the conserved Tyr at position 15. We have demonstrated that the Tyr 15 of FKBP22 is indispensable for preserving its dimerization ability, catalytic activity, and structure. The residue, however, little contributed to its inhibitor binding ability and stability. The mode of action of Tyr 15 has been discussed at length.
authors.affiliation: Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata 700054, West Bengal, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Polley
authors.affiliation: Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata 700054, West Bengal, India.
authors.forename: Devlina
authors.initials: D
authors.lastname: Chakravarty
authors.affiliation: Dr. B.C. Guha Centre for Genetic Engineering, University of Calcutta, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Gopal
authors.initials: G
authors.lastname: Chakrabarti
authors.affiliation: Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata 700054, West Bengal, India. Electronic address: subratasau@gmail.com.
authors.forename: Subrata
authors.initials: S
authors.lastname: Sau
mesh_terms: D019943:Amino Acid Substitution; D017124:Conserved Sequence; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology; D056004:Molecular Dynamics Simulation; D009154:Mutation; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D058767:Protein Unfolding; D022021:Tacrolimus Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D014443:Tyrosine; C403422:FKBP22; D022021:Tacrolimus Binding Proteins
keywords: FKBP22; Mip protein; N-terminal domain; PPIase and dimer; Tyrosine