title: Fabrication and detection of tissue engineered bone aggregates based on encapsulated human ADSCs within hybrid calcium alginate/bone powder gel-beads in a spinner flask.
abstract: Traditional treatment for bone diseases limits their clinical application due to undesirable host immune rejection, limited donator sources and severe pain and suffering for patients. Bone tissue engineering therefore is expected to be a more effective way in treating bone diseases. In the present study, hybrid calcium alginate/bone powder gel-beads with a uniform size distribution, good biocompatibility and osteoinductive capability, were prepared to be used as an in-vitro niche-like matrix. The beads were optimized using 2.5% (w/v) sodium alginate solution, 4.5% (w/v) CaCl2 solution and 5.0mg/mL bone powder using an easy-to-use method. Human ADSCs were cultured and induced into chondrocytes and osteoblasts, respectively. The cells were characterized by histological staining showing the ADSCs were able to maintain their characteristic morphology with multipotent differentiation ability. ADSCs at density of 5 × 10(6)cells/mL were encapsulated into the gel-beads aiming to explore cell expansion under different conditions and the osteogenic induction of ADSCs was verified by specific staining. Results demonstrated that the encapsulated ADSCs expanded 5.6 folds in 10 days under dynamic condition via spinner flask, and were able to differentiate into osteoblasts (OBs) with extensive mineralized nodules forming the bone aggregates over 3 weeks postosteogenic induction. In summary, hybrid gel-beads encapsulating ADSCs are proved to be feasible as a new method to fabricate tissue engineered bone aggregation with potential to treat skeletal injury in the near future.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address: Kedongsong@dlut.edu.cn.
authors.forename: Kedong
authors.initials: K
authors.lastname: Song
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Zeren
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
authors.forename: Shihao
authors.initials: S
authors.lastname: Feng
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Burns Research Group, ANZAC Research Institute, University of Sydney, Concord, NSW 2139, Australia.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Oncology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China. Electronic address: whwl@hotmail.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China. Electronic address: liutq@dlut.edu.cn.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Liu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000464:Alginates / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000737:chemistry / Q000502:physiology; D002122:Calcium Chloride / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005782:Gels / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D010316:Particle Size; D023822:Tissue Engineering
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D000469:Alkaline Phosphatase; D002122:Calcium Chloride
keywords: ADSCs; Bone aggregates; Bone tissue engineering; Dynamic condition; Hybrid gel-beads