title: Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences.
abstract: We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae from crayfish in Jeollanam-do Province, Korea. The metacercariae were round, 180 μm in average diameter, encysted with 2 layers of thick walls, but the stylet on the oral sucker was not clearly seen. The adult flukes were oval shape, and 760-820 μm long and 320-450 μm wide, with anterolateral location of 2 large testes. The phylogenetic tree based on ITS1 sequences of 6 M. spinulosus samples showed their distinguished position from other trematode species in GenBank. The most closely resembled group was Paragonimus spp. which also take crayfish or crabs as the second intermediate host. The present study is the first molecular characterization of M. spinulosus and provided a basis for further phylogenetic studies to compare with other trematode fauna in Korea.
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Hospital, Gwangju 61469, Korea.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Won
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Deok-Gyu
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Jaeeun
authors.initials: J
authors.lastname: Cho
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Bong-Kwang
authors.initials: BK
authors.lastname: Jung
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Min-Jae
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Preventive Medicine, Chonnam National University Medical School and Regional Cardiocerebrovascular Center, Gwangju 61469, Korea.
authors.forename: Yong Woon
authors.initials: YW
authors.lastname: Yun
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 03080, Korea.
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
authors.affiliation: Department of Laboratory Medicine, Chonnam National University Hospital, Gwangju 61469, Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Ryang
mesh_terms: D000818:Animals; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D058488:Metacercariae / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014200:Trematoda / Q000145:classification* / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S
keywords: ITS1; Macroorchis spinulosus; crayfish; sequencing