title: Effect of Corilagin on the miR-21/smad7/ERK signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model.
abstract: This study sought to investigate the anti-fibrotic effect of Corilagin via interference with the miR-21/smad7/ERK signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model. Mice were infected with Schistosoma japonicum cercaria to establish the mouse model of schistosomiasis-induced hepatic fibrosis. At four weeks after infection, the groups were given different medications. The living conditions were observed. Real-time PCR was employed to detect the mRNA levels of miR-21, smad7 and connective tissue growth factor (CTGF), and western blotting was used to examine the protein levels of smad7, CTGF, smad1, p-smad1, smad2, p-smad2, ERK1/2, p-ERK1/2 and TGF-β receptor I. Immunohistochemistry was used to examine the expression of CTGF. Compared with the model group, increasing concentrations of Corilagin improved the quality of life, inhibited the mRNA expression of miR-21, promoted smad7 protein expression, and inhibited CTGF protein expression (p<0.05 or 0.01). Moreover, Corilagin significantly reduced the protein levels of p-smad1, p-smad2, p-ERK1/2, and TGF-β receptor I (p<0.05 or 0.01). CTGF staining in the cytoplasm was markedly decreased by Corilagin (p<0.05 or 0.01). In conclusion, Corilagin inhibited schistosomiasis-induced hepatic fibrosis via the miR21/smad7/ERK pathway in this animal model.
authors.affiliation: Department of Hepatology, Hubei Provincial Hospital of Chinese Medicine, Wuhan 430074, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: School of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan 430060, PR China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xue
authors.affiliation: School of Life Science, Hubei University, Wuhan 430062, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ma
authors.affiliation: School of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan 430060, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Chen
authors.affiliation: School of Basic Medical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, PR China.
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Shang
authors.affiliation: School of Clinical Medicine, Guangxi University of Chinese Medicine, Nanning 530007, PR China.
authors.forename: Quan-Qiang
authors.initials: QQ
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China. Electronic address: leizhao@hust.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D055513:Connective Tissue Growth Factor / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000469:parasitology; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011788:Quality of Life; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D012549:Schistosoma japonicum / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012554:Schistosomiasis japonica / Q000188:drug therapy* / Q000469:parasitology; D051906:Smad7 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C526318:CCN2 protein, mouse; D005960:Glucosides; D047348:Hydrolyzable Tannins; C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs; D018125:Receptors, Transforming Growth Factor beta; D051906:Smad7 Protein; C497807:Smad7 protein, mouse; D055513:Connective Tissue Growth Factor; C049096:corilagin; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I
keywords: Corilagin; ERK; Liver fibrosis; Schistosomiasis; miR-21; smad7