title: Optogenetic Control of Fibroblast Growth Factor Receptor Signaling.
abstract: FGFR1 is a member of the fibroblast growth factor family, which controls diverse cellular functions such as cell proliferation, migration, and differentiation. OptoFGFR1, an optogenetic method to modulate the FGFR signaling pathway with light by utilizing PHR domain of cryptochrome2 and cytoplasmic region of FGFR1, enabled light-guided activation of FGFR to study its effects on downstream signaling pathway and during diverse biological processes such as cell migration. Here, we describe about optogenetic and microscopic methods to spatiotemporally manipulate FGFR signaling in a single cell or group of cells using confocal microscope and LED array.
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Seoul, 136-791, Republic of Korea.
authors.forename: Nury
authors.initials: N
authors.lastname: Kim
authors.affiliation: Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea.
authors.forename: Jin Man
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Seoul, 136-791, Republic of Korea. wondo@kaist.ac.kr.|Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea. wondo@kaist.ac.kr.
authors.forename: Won Do
authors.initials: WD
authors.lastname: Heo
mesh_terms: D015153:Blotting, Western / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D056931:Cryptochromes / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D062308:Optogenetics / Q000379:methods*; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection / Q000379:methods
chemical_list: C538966:CRY2 protein, human; D056931:Cryptochromes; C496348:FGFR1 protein, human; D051496:Receptor, Fibroblast Growth Factor, Type 1
keywords: FGFR; Live cell imaging; Migration; Optogenetics; Signaling pathway; Spatiotemporal regulation; optoFGFR1