title: Effect of selenite on the morphology and respiratory activity of Phanerochaete chrysosporium biofilms.
abstract: The temporal and spatial effects of selenite (SeO3(2-)) on the physical properties and respiratory activity of Phanerochaete chrysosporium biofilms, grown in flow-cell reactors, were investigated using oxygen microsensors and confocal laser scanning microscopy (CLSM) imaging. Exposure of the biofilm to a SeO3(2-) load of 1.67mgSeL(-1)h(-1) (10mgSeL(-1) influent concentration), for 24h, resulted in a 20% reduction of the O2 flux, followed by a ∼10% decrease in the glucose consumption rate. Long-term exposure (4days) to SeO3(2-) influenced the architecture of the biofilm by creating a more compact and dense hyphal arrangement resulting in a decrease of biofilm thickness compared to fungal biofilms grown without SeO3(2-). To the best of our knowledge, this is the first time that the effect of SeO3(2-) on the aerobic respiratory activity on fungal biofilms is described.
authors.affiliation: UNESCO-IHE Institute for Water Education, Westvest 7, 2611 AX Delft, The Netherlands. Electronic address: jime_na_es@yahoo.com.mx.
authors.forename: Erika J
authors.initials: EJ
authors.lastname: Espinosa-Ortiz
authors.affiliation: Université Paris-Est, Laboratoire Géomatériaux et Environnement (EA 4508), UPEM, 77454 Marne-la-Vallée, France.
authors.forename: Yoan
authors.initials: Y
authors.lastname: Pechaud
authors.affiliation: Center for Biofilm Engineering, Montana State University, 366 EPS, PO Box 173980, Bozeman, MT 59717, USA.
authors.forename: Ellen
authors.initials: E
authors.lastname: Lauchnor
authors.affiliation: UNESCO-IHE Institute for Water Education, Westvest 7, 2611 AX Delft, The Netherlands.
authors.forename: Eldon R
authors.initials: ER
authors.lastname: Rene
authors.affiliation: Center for Biofilm Engineering, Montana State University, 366 EPS, PO Box 173980, Bozeman, MT 59717, USA.
authors.forename: Robin
authors.initials: R
authors.lastname: Gerlach
authors.affiliation: Center for Biofilm Engineering, Montana State University, 366 EPS, PO Box 173980, Bozeman, MT 59717, USA.
authors.forename: Brent M
authors.initials: BM
authors.lastname: Peyton
authors.affiliation: Université Paris-Est, Laboratoire Géomatériaux et Environnement (EA 4508), UPEM, 77454 Marne-la-Vallée, France.
authors.forename: Eric D
authors.initials: ED
authors.lastname: van Hullebusch
authors.affiliation: UNESCO-IHE Institute for Water Education, Westvest 7, 2611 AX Delft, The Netherlands.
authors.forename: Piet N L
authors.initials: PN
authors.lastname: Lens
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D019149:Bioreactors / Q000382:microbiology; D010100:Oxygen / Q000494:pharmacology; D020075:Phanerochaete / Q000502:physiology*; D020887:Selenious Acid / Q000494:pharmacology*; D014865:Waste Disposal, Fluid
chemical_list: D020887:Selenious Acid; D010100:Oxygen
keywords: Fungal biofilm; Microelectrodes; Oxygen profiles; Phanerochaete chrysosporium; Selenium