title: Interaction between Esophageal Squamous Cell Carcinoma and Adipose Tissue in Vitro.
abstract: Esophageal squamous cell carcinoma (ESCC) develops within the squamous epithelial layer and invades the submucosa to the subadventitia that has adipose tissue (AT). AT seems critical to ESCC progression, but the underlying mechanism is unknown. We aimed to address the association between ESCC and AT in vitro. ESCC cells were cultured on rat or human subcutaneous AT-embedded or -non-embedded collagen gel. AT promoted the growth of ESCC cells and inhibited their apoptosis. AT promoted the expression of the squamous differentiation marker involucrin in ESCC cells. AT accelerated the expression of invasion-related factors in poorly differentiated ESCC cells only. AT promoted the expression of phosphorylated-insulin-like growth factor-1 receptor in ESCC cells, whereas it inhibited that of the human epidermal growth factor receptor 2. Insulin-like growth factor-1, but not leptin, adiponectin, or resistin, promoted and inhibited the growth and apoptosis of ESCC cells, respectively. In turn, ESCC cells decreased the production of these adipokines in AT and the number of preadipocytes and mesenchymal stem cell-like cells, which developed from AT. These results suggest that i) AT may influence the progression of ESCC with increased growth or invasion and decreased apoptosis through insulin-like growth factor-1/insulin-like growth factor-1 receptor signaling, ii) AT may affect human epidermal growth factor receptor 2-targeted therapy; and iii) the cancer cells may affect adipokine production in AT.
authors.affiliation: Department of Internal Medicine and Gastrointestinal Endoscopy, Faculty of Medicine, Saga University, Saga, Japan; Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan. Electronic address: 10624022@edu.cc.saga-u.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakayama
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Aoki
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Uchihashi
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Nishijima-Matsunobu
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Mihoko
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Department of Basic Science of Nursing, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Nahoko
authors.initials: N
authors.lastname: Kakihara
authors.affiliation: Department of Internal Medicine and Gastrointestinal Endoscopy, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Iwakiri
authors.affiliation: Department of Internal Medicine and Gastrointestinal Endoscopy, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Fujimoto
authors.affiliation: Department of Pathology and Microbiology, Faculty of Medicine, Saga University, Saga, Japan. Electronic address: todas@cc.saga-u.ac.jp.
authors.forename: Shuji
authors.initials: S
authors.lastname: Toda
mesh_terms: D052242:Adiponectin / Q000494:pharmacology; D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000503:physiopathology*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D004938:Esophageal Neoplasms / Q000503:physiopathology*; D000077277:Esophageal Squamous Cell Carcinoma; D064448:Filamins / Q000378:metabolism; D006801:Humans; D006984:Hypertrophy / Q000503:physiopathology; D007334:Insulin-Like Growth Factor I; D050356:Lipid Metabolism / Q000502:physiology; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008854:Microscopy, Electron; D011498:Protein Precursors / Q000378:metabolism; D017208:Rats, Wistar; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D052243:Resistin / Q000494:pharmacology; D017154:Stromal Cells / Q000502:physiology; D014407:Tumor Cells, Cultured; D000097794:Kalinin
chemical_list: D052242:Adiponectin; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D064448:Filamins; D007334:Insulin-Like Growth Factor I; D053511:Matrix Metalloproteinase 14; D020780:Matrix Metalloproteinase 9; D011498:Protein Precursors; D018719:Erb-b2 Receptor Tyrosine Kinases; D052243:Resistin; D000097794:Kalinin; C032253:involucrin; C508053:ERBB2 protein, human