title: The immunomodulatory properties of human bone marrow-derived mesenchymal stromal cells are defined according to multiple immunobiological criteria.
abstract: OBJECTIVE
  Human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) are well known to modulate T cells. However, the molecular mechanisms that mark hBM-MSCs immunomodulation of T cells are not fully resolved.
  
  
  METHODS
  hBM-MSCs harvested from sternum or iliac crest of five healthy donors and characterized in accordance with the International Society of Cellular Therapy (ISCT) guidelines are co-cultured with T cells. Additionally, modulatory effects of MSCs on T-cell viability, proliferation, cytokine profile, co-stimulatory pathway, activation and immunomodulation are also determined.
  
  
  RESULTS
  hBM-MSCs significantly reduced the expression of T-cell activation marker CD38 as well as co-stimulatory markers CD134 and CD154, whilst that of CD27 remained unchanged. BrdU, CFSE and Ki67 proliferation assays showed that hBM-MSCs reduced T-cell proliferation. Moreover, viability of T cells remained unchanged when co-cultured with hBM-MSCs. Finally, T cells when co-cultured with hBM-MSCs showed increased secretion of IL-10 and IL-11.
  
  
  CONCLUSIONS
  Collectively, hBM-MSCs are able to modulate the main steps involved in T-cell response toward a tolerogenic state. Thus, establishing immunobiological criteria defining the immunosuppressive effect of hBM-MSCs is of importance to reach efficient immunotherapeutic intervention.
authors.affiliation: Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences I, Lebanese University, Hadath, Lebanon.
authors.forename: Hussein
authors.initials: H
authors.lastname: Fayyad-Kazan
authors.affiliation: School of Medicine, Lebanese American University, P.O. Box 36, Byblos, Lebanon.
authors.forename: Wissam H
authors.initials: WH
authors.lastname: Faour
authors.affiliation: Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences I, Lebanese University, Hadath, Lebanon. bassam.badran@ul.edu.lb.
authors.forename: Bassam
authors.initials: B
authors.lastname: Badran
authors.affiliation: Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles (ULB), Campus Erasme, Bâtiment de Transfusion (Level +1), Route de Lennik n° 808, 1070, Brussels, Belgium.
authors.forename: Laurence
authors.initials: L
authors.lastname: Lagneaux
authors.affiliation: Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles (ULB), Campus Erasme, Bâtiment de Transfusion (Level +1), Route de Lennik n° 808, 1070, Brussels, Belgium.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Najar
mesh_terms: D017209:Apoptosis; D001853:Bone Marrow; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D006801:Humans; D056747:Immunomodulation; D016753:Interleukin-10 / Q000276:immunology; D017370:Interleukin-11 / Q000276:immunology; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: C508609:IL10 protein, human; C508611:IL11 protein, human; D017370:Interleukin-11; D016753:Interleukin-10
keywords: Activation; Co-stimulation; Cytokine; Immunobiological criteria; Immunogenicity; MSC; Proliferation; T cell