title: An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber.
abstract: We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polymorphisms from bulked F2 pools identified the causative variant occurring in Csa4G126690. Csa4G126690 shows high homology to Arabidopsis SEPALLATA2 (SEP2) thus being designated CsSEP2. The causative variant was located on the splicing site of CsSEP2, resulting in the skipping of exon 6 and abolishment of the transcriptional activity. Our data suggest that CsSEP2 is involved in the floral organ and fruits development by conferring transcriptional activity.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China.|Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.|Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Gao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Jinjing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: YaoYao
authors.initials: Y
authors.lastname: Lun
authors.affiliation: Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
authors.forename: Jianshu
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Shenhao
authors.initials: S
authors.lastname: Wang
authors.affiliation: Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Qingzhi
authors.initials: Q
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China. wangxff99@nwsuaf.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China. huangsanwen@caas.cn.|Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. huangsanwen@caas.cn.
authors.forename: Sanwen
authors.initials: S
authors.lastname: Huang
mesh_terms: D001483:Base Sequence; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development*; D005091:Exons / Q000235:genetics*; D035264:Flowers / Q000235:genetics / Q000254:growth & development*; D005638:Fruit / Q000235:genetics / Q000254:growth & development*; D017343:Genes, Plant; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D010940:Plant Proteins
keywords: Cucumis sativus L; EMS mutant; exon skipping; floral and fruits development; whole genome re-sequencing