title: Surfactant-free poly(lactide-co-glycolide) nanoparticles for improving in vitro anticancer efficacy of tetrandrine.
abstract: The objective of this study was to improve the efficacy of a natural compound tetrandrine against cancer by designing surfactant-free poly(lactic-co-glycolic acid) (PLGA) nanoparticles as drug carriers for tetrandrine. Nanoparticles were prepared from PLGA via the nano-precipitation method with or without the presence of surfactant poly(vinyl alcohol) (PVA) to encapsulate tetrandrine. Tetrandrine-loaded surfactant-free PLGA nanoparticles had an average particle size of 169.3 nm and morphology similar to the PLGA nanoparticles prepared using PVA as the surfactant. Tetrandrine-loaded surfactant-free PLGA nanoparticles could retard drug release in phosphate buffered saline (PBS) at pH 7.4 and the cumulative release of tetrandrine reached up to 68.33% over a period of 120 h. A549 cell line was used as the model cancer cells to investigate anticancer capability of tetrandrine-loaded surfactant-free PLGA nanoparticles via apoptosis assay, cytotoxicity and lysosome injury studies. The results showed that tetrandrine-loaded surfactant-free PLGA nanoparticles could effectively reduce cell viability and synergistically enhance tetrandrine-induced cell apoptosis.
authors.affiliation: a Department of Pharmacy, Union Hospital , Tongji Medical College of Huazhong University of Science and Technology , Wuhan , China ;
authors.forename: Chen
authors.initials: C
authors.lastname: Shi
authors.affiliation: b Department of Pharmacy , Huazhong University of Science and Technology , Wuhan , China ;
authors.forename: Fang
authors.initials: F
authors.lastname: Zeng
authors.affiliation: c Department of Orthopedics, Union Hospital, Tongji Medical College , Huazhong University of Science and Technology , Wuhan , China.
authors.forename: Dehao
authors.initials: D
authors.lastname: Fu
mesh_terms: D000072283:A549 Cells; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D044182:Benzylisoquinolines / Q000008:administration & dosage* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy; D053758:Nanoparticles / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044182:Benzylisoquinolines; D003692:Delayed-Action Preparations; C009438:tetrandrine; D011098:Polyglactin 910
keywords: Apoptosis; PLGA; lysosomes; surfactant-free nanoparticles; tetrandrine