title: Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent.
abstract: A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USA.
authors.forename: Sanhita
authors.identifier: http://orcid.org/0000-0001-9910-996X
authors.initials: S
authors.lastname: Sinharay
authors.affiliation: Department of Pharmaceutical Sciences, University of Arizona, Tucson, AZ, USA.
authors.forename: Gabriela
authors.identifier: http://orcid.org/0000-0001-6722-0823
authors.initials: G
authors.lastname: Fernández-Cuervo
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USA.
authors.forename: Jasmine P
authors.initials: JP
authors.lastname: Acfalle
authors.affiliation: Department of Medical Imaging, University of Arizona, 1515 N. Campbell Ave., Tucson, AZ, USA. mpagel@u.arizona.edu.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Pagel
mesh_terms: D055598:Chemical Phenomena; D003287:Contrast Media / Q000737:chemistry* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D013429:Sulfatases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003287:Contrast Media; D013429:Sulfatases
keywords: NMR imaging; diagnostic agents; enzyme kinetics; kinetics; sulfatases