title: Diversity-Oriented Combinatorial Biosynthesis of Hybrid Polyketide Scaffolds from Azaphilone and Benzenediol Lactone Biosynthons.
abstract: Two disparate polyketide families, the benzenediol lactones and the azaphilones, are produced by fungi using iterative polyketide synthase (iPKS) enzymes consisting of collaborating partner subunits. Exploitation of this common biosynthetic logic using iPKS subunit shuffling allowed the diversity-oriented combinatorial biosynthesis of unprecedented polyketide scaffolds new to nature, bearing structural motifs from both of these orthogonal natural product families. Starter unit acyltransferase domain replacements proved necessary but not sufficient to guarantee communication between iPKS subunits.
authors.affiliation: Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, Beijing 100081, P. R. China.|Natural Products Center, School of Natural Resources and the Environment, The University of Arizona , 250 East Valencia Road, Tucson, Arizona 85706, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.affiliation: Natural Products Center, School of Natural Resources and the Environment, The University of Arizona , 250 East Valencia Road, Tucson, Arizona 85706, United States.|State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University , 24 Tong Jia Xiang, Nanjing 210009, P. R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Natural Products Center, School of Natural Resources and the Environment, The University of Arizona , 250 East Valencia Road, Tucson, Arizona 85706, United States.
authors.forename: Ya-ming
authors.initials: YM
authors.lastname: Xu
authors.affiliation: Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, Beijing 100081, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, Beijing 100081, P. R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, Beijing 100081, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.affiliation: Natural Products Center, School of Natural Resources and the Environment, The University of Arizona , 250 East Valencia Road, Tucson, Arizona 85706, United States.
authors.forename: A A Leslie
authors.initials: AA
authors.lastname: Gunatilaka
authors.affiliation: Biotechnology Research Institute, The Chinese Academy of Agricultural Sciences , 12 Zhongguancun South Street, Beijing 100081, P. R. China.
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Natural Products Center, School of Natural Resources and the Environment, The University of Arizona , 250 East Valencia Road, Tucson, Arizona 85706, United States.
authors.forename: István
authors.initials: I
authors.lastname: Molnár
mesh_terms: D001578:Benzopyrans / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D015394:Molecular Structure; D010860:Pigments, Biological / Q000737:chemistry*; D048630:Polyketide Synthases / Q000378:metabolism*; D061065:Polyketides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D001578:Benzopyrans; D007783:Lactones; D010860:Pigments, Biological; D061065:Polyketides; C494154:azaphilone; D048630:Polyketide Synthases