title: Age-related energy values of bakery meal for broiler chickens determined using the regression method.
abstract: A study was conducted to determine the ileal digestible energy (IDE), ME, and MEn contents of bakery meal using the regression method and to evaluate whether the energy values are age-dependent in broiler chickens from zero to 21 d post hatching. Seven hundred and eighty male Ross 708 chicks were fed 3 experimental diets in which bakery meal was incorporated into a corn-soybean meal-based reference diet at zero, 100, or 200 g/kg by replacing the energy-yielding ingredients. A 3 × 3 factorial arrangement of 3 ages (1, 2, or 3 wk) and 3 dietary bakery meal levels were used. Birds were fed the same experimental diets in these 3 evaluated ages. Birds were grouped by weight into 10 replicates per treatment in a randomized complete block design. Apparent ileal digestibility and total tract retention of DM, N, and energy were calculated. Expression of mucin (MUC2), sodium-dependent phosphate transporter (NaPi-IIb), solute carrier family 7 (cationic amino acid transporter, Y(+) system, SLC7A2), glucose (GLUT2), and sodium-glucose linked transporter (SGLT1) genes were measured at each age in the jejunum by real-time PCR. Addition of bakery meal to the reference diet resulted in a linear decrease in retention of DM, N, and energy, and a quadratic reduction (P < 0.05) in N retention and ME. There was a linear increase in DM, N, and energy as birds' ages increased from 1 to 3 wk. Dietary bakery meal did not affect jejunal gene expression. Expression of genes encoding MUC2, NaPi-IIb, and SLC7A2 linearly increased (P < 0.05) with age. Regression-derived MEn of bakery meal linearly increased (P < 0.05) as the age of birds increased, with values of 2,710, 2,820, and 2,923 kcal/kg DM for 1, 2, and 3 wk, respectively. Based on these results, utilization of energy and nitrogen in the basal diet decreased when bakery meal was included and increased with age of broiler chickens.
authors.affiliation: Department of Animal Sciences, Purdue University, 915 W. State St. West Lafayette, IN 47907-2054. Electronic address: catarinastefanello@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Stefanello
authors.affiliation: Departament of Animal Science, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 7712, Porto Alegre, RS, Brazil, 91540-000.
authors.forename: S L
authors.initials: SL
authors.lastname: Vieira
authors.affiliation: Department of Animal Sciences, Purdue University, 915 W. State St. West Lafayette, IN 47907-2054.
authors.forename: P
authors.initials: P
authors.lastname: Xue
authors.affiliation: Department of Animal Sciences, Purdue University, 915 W. State St. West Lafayette, IN 47907-2054.
authors.forename: K M
authors.initials: KM
authors.lastname: Ajuwon
authors.affiliation: Department of Animal Sciences, Purdue University, 915 W. State St. West Lafayette, IN 47907-2054.
authors.forename: O
authors.initials: O
authors.lastname: Adeola
mesh_terms: D000367:Age Factors; D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004734:Energy Metabolism; D015870:Gene Expression; D007422:Intestines / Q000502:physiology; D008297:Male; D012044:Regression Analysis
chemical_list: D030161:Avian Proteins
keywords: age; bakery meal; broiler; gene expression; metabolizable energy