title: The Histone Deacetylase Complex 1 Protein of Arabidopsis Has the Capacity to Interact with Multiple Proteins Including Histone 3-Binding Proteins and Histone 1 Variants.
abstract: Intrinsically disordered proteins can adopt multiple conformations, thereby enabling interaction with a wide variety of partners. They often serve as hubs in protein interaction networks. We have previously shown that the Histone Deacetylase Complex 1 (HDC1) protein from Arabidopsis (Arabidopsis thaliana) interacts with histone deacetylases and quantitatively determines histone acetylation levels, transcriptional activity, and several phenotypes, including abscisic acid sensitivity during germination, vegetative growth rate, and flowering time. HDC1-type proteins are ubiquitous in plants, but they contain no known structural or functional domains. Here, we explored the protein interaction spectrum of HDC1 using a quantitative bimolecular fluorescence complementation assay in tobacco (Nicotiana benthamiana) epidermal cells. In addition to binding histone deacetylases, HDC1 directly interacted with histone H3-binding proteins and corepressor-associated proteins but not with H3 or the corepressors themselves. Surprisingly, HDC1 also was able to interact with variants of the linker histone H1. Truncation of HDC1 to the ancestral core sequence narrowed the spectrum of interactions and of phenotypic outputs but maintained binding to a H3-binding protein and to H1. Thus, HDC1 provides a potential link between H1 and histone-modifying complexes.
authors.affiliation: Plant Science Group, MCSB, MVLS, University of Glasgow, Glasgow G12 8QQ, United Kingdom (G.P., C.C., M.A.A.-F., N.A.D., K.P., A.A.); andBayer CropScience, B-9052 Ghent, Belgium (M.A.H.).
authors.forename: Giorgio
authors.identifier: 0000-0001-8972-1733
authors.initials: G
authors.lastname: Perrella
authors.affiliation: Plant Science Group, MCSB, MVLS, University of Glasgow, Glasgow G12 8QQ, United Kingdom (G.P., C.C., M.A.A.-F., N.A.D., K.P., A.A.); andBayer CropScience, B-9052 Ghent, Belgium (M.A.H.).
authors.forename: Craig
authors.initials: C
authors.lastname: Carr
authors.affiliation: Plant Science Group, MCSB, MVLS, University of Glasgow, Glasgow G12 8QQ, United Kingdom (G.P., C.C., M.A.A.-F., N.A.D., K.P., A.A.); andBayer CropScience, B-9052 Ghent, Belgium (M.A.H.).
authors.forename: Maria A
authors.initials: MA
authors.lastname: Asensi-Fabado
authors.affiliation: Plant Science Group, MCSB, MVLS, University of Glasgow, Glasgow G12 8QQ, United Kingdom (G.P., C.C., M.A.A.-F., N.A.D., K.P., A.A.); andBayer CropScience, B-9052 Ghent, Belgium (M.A.H.).
authors.forename: Naomi A
authors.initials: NA
authors.lastname: Donald
authors.affiliation: Plant Science Group, MCSB, MVLS, University of Glasgow, Glasgow G12 8QQ, United Kingdom (G.P., C.C., M.A.A.-F., N.A.D., K.P., A.A.); andBayer CropScience, B-9052 Ghent, Belgium (M.A.H.).
authors.forename: Katalin
authors.identifier: 0000-0002-2861-2266
authors.initials: K
authors.lastname: Páldi
authors.affiliation: Plant Science Group, MCSB, MVLS, University of Glasgow, Glasgow G12 8QQ, United Kingdom (G.P., C.C., M.A.A.-F., N.A.D., K.P., A.A.); andBayer CropScience, B-9052 Ghent, Belgium (M.A.H.).
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Hannah
authors.affiliation: Plant Science Group, MCSB, MVLS, University of Glasgow, Glasgow G12 8QQ, United Kingdom (G.P., C.C., M.A.A.-F., N.A.D., K.P., A.A.); andBayer CropScience, B-9052 Ghent, Belgium (M.A.H.) anna.amtmann@glasgow.ac.uk.
authors.forename: Anna
authors.initials: A
authors.lastname: Amtmann
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D035264:Flowers / Q000235:genetics; D020869:Gene Expression Profiling; D005822:Genetic Vectors; D018525:Germination; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D060066:Protein Interaction Maps; D014026:Nicotiana / Q000254:growth & development / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C000597113:HDC1 protein, Arabidopsis; D006657:Histones; D009687:Nuclear Proteins; D006655:Histone Deacetylases