title: Isolation, characterization and PCR multiplexing of microsatellite loci for two sub-species of terrestrial isopod Porcellio dilatatus (Crustacea, Oniscidea).
abstract: Several microsatellite markers have already been developed for different terrestrial isopod species such as Armadillidium vulgare, A. nasatum and Porcellionides pruinosus. In all these species, the endosymbiont Wolbachia has a feminizing effect that generates a female bias in sex ratio and reduces the number of reproductive males. Thus this can potentially decrease the genetic diversity of host populations. However, in some other isopod species, Wolbachia induces cytoplasmic incompatibility (CI); the most commonly described effect of Wolbachia in arthropods. The CI by rendering some crossings incompatible can reduce the gene flow and strengthen genetic differentiation between isopod populations. To date, the influence of Wolbachia inducing CI on population structure of terrestrial isopods has never been investigated. In this study, we developed 10 polymorphic microsatellite markers shared by two sub-species of Porcellio dilatatus. Crossings between the two sub-species are partially incompatible due to two CI-inducing Wolbachia strains. These new microsatellite markers will allow us to investigate the effect of CI on host genetic differentiation in this species complex.
authors.affiliation: Laboratoire EBI Ecologie & Biologie des Interactions - UMR CNRS 7267, Equipe Ecologie, Evolution, Symbiose, Université de Poitiers, Bât. B8-B35, 5 rue Albert Turpin, TSA 51106, 86022, Poitiers Cedex 9, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Michaud
authors.affiliation: Laboratoire EBI Ecologie & Biologie des Interactions - UMR CNRS 7267, Equipe Ecologie, Evolution, Symbiose, Université de Poitiers, Bât. B8-B35, 5 rue Albert Turpin, TSA 51106, 86022, Poitiers Cedex 9, France.
authors.forename: Cassandre
authors.initials: C
authors.lastname: Chupeau
authors.affiliation: Laboratoire EBI Ecologie & Biologie des Interactions - UMR CNRS 7267, Equipe Ecologie, Evolution, Symbiose, Université de Poitiers, Bât. B8-B35, 5 rue Albert Turpin, TSA 51106, 86022, Poitiers Cedex 9, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bech
authors.affiliation: Laboratoire EBI Ecologie & Biologie des Interactions - UMR CNRS 7267, Equipe Ecologie, Evolution, Symbiose, Université de Poitiers, Bât. B8-B35, 5 rue Albert Turpin, TSA 51106, 86022, Poitiers Cedex 9, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Thierry
authors.affiliation: Institut des Sciences de l'Evolution de Montpellier (UMR CNRS-IRD-UM 5554), Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Sicard
authors.affiliation: Laboratoire EBI Ecologie & Biologie des Interactions - UMR CNRS 7267, Equipe Ecologie, Evolution, Symbiose, Université de Poitiers, Bât. B8-B35, 5 rue Albert Turpin, TSA 51106, 86022, Poitiers Cedex 9, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Greve
authors.affiliation: Laboratoire EBI Ecologie & Biologie des Interactions - UMR CNRS 7267, Equipe Ecologie, Evolution, Symbiose, Université de Poitiers, Bât. B8-B35, 5 rue Albert Turpin, TSA 51106, 86022, Poitiers Cedex 9, France. sophie.beltran@univ-poitiers.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Beltran-Bech
mesh_terms: D000818:Animals; D005260:Female; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D033321:Isopoda / Q000235:genetics* / Q000382:microbiology; D008297:Male; D018895:Microsatellite Repeats; D010802:Phylogeny; D020577:Wolbachia
chemical_list: D005819:Genetic Markers
keywords: Cytoplasmic incompatibility; Isopods; Molecular markers; Porcellio dilatatus dilatatus; Porcellio dilatatus petiti; Wolbachia