title: Early alterations on photosynthesis-related parameters in Chlamydomonas reinhardtii cells exposed to atrazine: A multiple approach study.
abstract: Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide atrazine for 3h. Physiological cellular parameters, such as chlorophyll a fluorescence and oxidative stress monitored by flow cytometry and pigments levels were altered in microalgal cells exposed to 0.25 μM of atrazine. Furthermore, the effects of this herbicide on C. reinhardtii were explored using "omics" techniques. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 9 differentially expressed genes, related to photosynthesis, between control cultures and atrazine exposed cultures. Proteomic profiles were obtained using iTRAQ tags and MALDI-MS/MS analysis, identifying important changes in the proteome during atrazine stress; 5 proteins related to photosynthesis were downexpressed. The results of these experiments advance the understanding of photosynthetic adjustments that occur during an early herbicide exposure. Inhibition of photosynthesis induced by atrazine toxicity will affect the entire physiological and biochemical states of microalgal cells.
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira, s/n 15071 A Coruña, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Esperanza
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira, s/n 15071 A Coruña, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Seoane
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira, s/n 15071 A Coruña, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rioboo
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira, s/n 15071 A Coruña, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Herrero
authors.affiliation: Laboratorio de Microbiología, Facultad de Ciencias, Universidad de A Coruña, Campus de A Zapateira, s/n 15071 A Coruña, Spain. Electronic address: angeles.cid@udc.es.
authors.forename: Ángeles
authors.initials: Á
authors.lastname: Cid
mesh_terms: D001280:Atrazine / Q000633:toxicity*; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D000077194:Chlorophyll A; D006540:Herbicides / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D006540:Herbicides; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D001280:Atrazine; D000077194:Chlorophyll A
keywords: Atrazine; Flow cytometry; MALDI; Microalga; RNA-Seq; iTRAQ