title: Curcumin protects against the intestinal ischemia-reperfusion injury: involvement of the tight junction protein ZO-1 and TNF-α related mechanism.
abstract: Present study aimed to investigate the eff ect of curcumin-pretreatment on intestinal I/R injury and on intestinal mucosa barrier. Thirty Wistar rats were randomly divided into: sham, I/R, and curcumin groups (n=10). Animals in curcumin group were pretreated with curcumin by gastric gavage (200 mg/kg) for 2 days before I/R. Small intestine tissues were prepared for Haematoxylin & Eosin (H&E) staining. Serum diamine oxidase (DAO) and tumor necrosis factor (TNF)-α levels were measured. Expression of intestinal TNF-α and tight junction protein (ZO-1) proteins was detected by Western blot and/or immunohistochemistry. Serum DAO level and serum and intestinal TNF-α leves were signifi cantly increased after I/R, and the values were markedly reduced by curcumin pretreatment although still higher than that of sham group (p<0.05 or p<0.001). H&E staining showed the significant injury to intestinal mucosa following I/R, and curcumin pretreatment signifi cantly improved the histological structure of intestinal mucosa. I/R insult also induced significantly down-regulated expression of ZO-1, and the eff ect was dramatically attenuated by curcumin-pretreatment. Curcumin may protect the intestine from I/R injury through restoration of the epithelial structure, promotion of the recovery of intestinal permeability, as well as enhancement of ZO-1 protein expression, and this eff ect may be partly attributed to the TNF-α related pathway.
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Tian
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Sichen
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Jiang
keywords: Curcumin; Intestinal mucosa barrier; Intestinal permeability; Intestine; Ischemia/reperfusion (I/R) injury; Tight junction protein (ZO-1)