title: Isolation and Characterization of Chemopreventive Agent from Sphaeranthus amaranthoides Burm F.
abstract: OBJECTIVE
  To investigate the in vitro cytotoxic effect and to isolate and characterize a chemopreventive secondary metabolite from Sphaeranthus amaranthoides Burm F (sivakaranthai).
  
  
  METHODS
  In vitro cytotoxic effect was carried out by 3 (4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide assay. Different concentrations of the extracts were tested on three different cell lines namely A549, HT29, and MCF7. The chloroform extract was subjected to column chromatography, and the isolated compound was characterized by various spectral methods and by single crystal X-ray crystallography.
  
  
  RESULTS
  The concentration that cause 50% growth inhibition value of chloroform extract was found to be 0.9 and 19 μg/mL against MCF7 and A549 cell lines, respectively. Chloroform extract was subjected to column chromatography for the isolation of phytoconstituent. The structure of the isolated compound was identified by spectroscopic techniques such as infrared, nuclear magnetic resonance, XRD, and mass spectroscopy. On comparison of complete spectral detail of the compound, the proposed structure was identified as chrysosplenol D (a flavonoid). Chrysosplenol D was isolated for the first time from this plant.
  
  
  CONCLUSIONS
  The chloroform extract had higher cytotoxic effect, and the isolated chrysosplenol D may be responsible for the anti-proliferative effect of the plant.
  
  
  CONCLUSIONS
  The plant Sphaeranthus amaranthoides Burm F was extracted with solvents of increasing polarity. The chloroform extract was found to have cell inhibition towards MCF 7 and HT 29 cell lines. This was subjected to fractionation. Chrysosplenol D was isolated from the chloroform extract.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur, Chennai, India.
authors.forename: S
authors.initials: S
authors.lastname: Gayatri
authors.affiliation: Department of Medicinal Chemistry, Greensmed Labs, Thuraipakkam, Chennai, India.
authors.forename: R
authors.initials: R
authors.lastname: Suresh
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur, Chennai, India.
authors.forename: C Uma Maheswara
authors.initials: CU
authors.lastname: Reddy
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sri Ramachandra University, Porur, Chennai, India.
authors.forename: K
authors.initials: K
authors.lastname: Chitra
keywords: 3 (4; 5-dimethylthiazol-2-yl) 2; 5-diphenyltetrazolium bromide assay; Characterization; Chemopreventive agent; Chrysosplenol D; Isolation; Sphaeranthus amaranthoides