title: Effect of High MiR-146a Expression on the Inflammatory Reaction in BV2 Cells.
abstract: OBJECTIVE
  To explore the effect of MiR-146a regulator function on the inflammatory response in neuroglia cell (microglia).
  
  
  METHODS
  BV2 cells were transfected by MiR-146a mimics,and then stimulated by lipopolysaccharide (LPS). MiR-146a expression was measured by real-time polymerase chain reaction (real-time PCR). Interleukin (IL)-6 and tumor necrosis factor α (TNFα) were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, IL-1 receptor-associated kinase 1 (IRAK1) and TNF receptor-associated factor 6 (TRAF6) were detected by PCR and Western blotting.
  
  
  RESULTS
  Compared to the normal control group, MiR-146a expression was significantly elevated by transfection with MiR-146a mimics (t=5.846, P=0.0021). The expression levels of IRAK1, TRAF6, TNFα, and IL-6 significantly increased in the LPS-stimulated BV2 cells compared to the non-stimulated BV2. The enhancement of MiR-146a resulted in significantly decreased IL-6 (t=5.200, P=0.0003) and TNFα (t=9.812, P<0.0001) secretion. The mRNA (t=5.353, P=0.0007) and protein (t=6.980, P=0.0009) levels of TRAF6, but not IRAK1, also significantly decreased.
  
  
  CONCLUSIONS
  MiR-146a may negatively suppress the inflammatory response of BV2 cells by regulating the expression of IRAF6 molecules in the TLR4 signaling pathway.
authors.affiliation: Department of Anesthesiology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Le
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Anatomy,Histology and Embryology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Hao-wu
authors.initials: HW
authors.lastname: Jiang
authors.affiliation: Department of Anatomy,Histology and Embryology,Institute of Basic Medical Sciences,CAMS and PUMC,Beijing 100005,China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Yu-guang
authors.initials: YG
authors.lastname: Huang
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007249:Inflammation; D053592:Interleukin-1 Receptor-Associated Kinases; D015850:Interleukin-6; D008070:Lipopolysaccharides; D035683:MicroRNAs; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D008070:Lipopolysaccharides; D035683:MicroRNAs; D012333:RNA, Messenger; D048029:TNF Receptor-Associated Factor 6; D014409:Tumor Necrosis Factor-alpha; C507768:IRAK1 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases