title: Discovery of novel feruloyl esterase activity of BioH in Escherichia coli BL21(DE3).
abstract: OBJECTIVE
  To characterize a novel feruloyl esterase from Escherichia coli BL21 DE3.
  
  
  RESULTS
  The gene encoding BioH was cloned and overexpressed in E. coli. The protein was purified and its catalytic activity was assessed. BioH exhibited feruloyl esterase activity toward a broad range of substrates, and the corresponding kinetic constants for the methyl ferulate, ethyl ferulate, and methyl p-coumarate substrates were: K m values of 0.48, 6.3, and 1.9 mM, respectively, and k cat /K m values of 9.3, 3.8, and 3.8 mM(-1) s(-1), respectively.
  
  
  CONCLUSIONS
  Feruloyl esterase from E. coli was expressed for the first time. BioH was confirmed to be a feruloyl esterase.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.
authors.forename: Le
authors.initials: L
authors.lastname: Kang
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, Shanxi, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Bai
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China. yjcai@jiangnan.edu.cn.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Cai
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, Shanxi, China. zhengxh@nwu.edu.cn.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng
mesh_terms: D002109:Caffeic Acids / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D007700:Kinetics; D013379:Substrate Specificity
chemical_list: D002109:Caffeic Acids; D029968:Escherichia coli Proteins; C061417:bioH protein, E coli; C099085:ethyl ferulate; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; C530806:methyl ferulate
keywords: BioH esterase; Escherichia coli; Feruloyl esterase