title: CmWRKY1 Enhances the Dehydration Tolerance of Chrysanthemum through the Regulation of ABA-Associated Genes.
abstract: WRKY transcription factors serve as antagonistic or synergistic regulators in a variety of abiotic stress responses in plants. Here, we show that CmWRKY1, a member of the group IIb WRKY family isolated from Chrysanthemum morifolium, exhibits no transcriptional activation in yeast cells. The subcellular localization examination showed that CmWRKY1 localizes to the nucleus in vivo. Furthermore, CmWRKY1-overexpressing transgenic lines exhibit enhanced dehydration tolerance in response to polyethylene glycol (PEG) treatment compared with wild-type plants. We further confirmed that the transgenic plants exhibit suppressed expression levels of genes negatively regulated by ABA, such as PP2C, ABI1 and ABI2, and activated expression levels of genes positively regulated by ABA, such as PYL2, SnRK2.2, ABF4, MYB2, RAB18, and DREB1A. Taken together, our results indicate that CmWRKY1 plays an important role in the response to drought in chrysanthemum through an ABA-mediated pathway.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.|Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing, 210095, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Fan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Hainan
authors.initials: H
authors.lastname: Sun
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yinjie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.|Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing, 210095, China.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
mesh_terms: D000040:Abscisic Acid / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics*; D031202:Chrysanthemum / Q000235:genetics* / Q000254:growth & development; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D003681:Dehydration / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D030821:Plants, Genetically Modified; D016415:Sequence Alignment; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D015533:Transcriptional Activation / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C499621:ZAP1 protein, Arabidopsis; D000040:Abscisic Acid