title: Rapidly increased vasopressin promotes acute platelet aggregation and early brain injury after experimental subarachnoid hemorrhage in a rat model.
abstract: To investigate the dynamic expression of vasopressin and its potential role in rat brain tissue after experimental subarachnoid hemorrhage (SAH).
  
  
  
  Male Sprague-Dawley rats were divided into 10min, 1h, 6h, 24h, 48h and 72h groups. The SAH model was established by endovascular puncture. ELISA and immunohistochemistry were performed to evaluate dynamic expression of vasopressin. Immunohistochemistry of GPIIb/IIIa integrin was used to assess platelet aggregation. Double immunofluorescence labeling was carried out to observe the reaction between vasopressin and platelet. Early brain injury was evaluated by apoptotic cells counting. Neurobehavioral score was performed to assess neuroprotective role of SR 49059 (a selective antagonists of vasopressin receptor).
  
  
  
  In peripheral blood and hypothalamus, vasopressin increased rapidly at 6h and 24h. Expression of GPIIb/IIIa integrin peaked at 24h in cortex and hippocampus. Immunofluorescence showed that vasopressin and GPIIb/IIIa integrin located at the same site. Administration of SR 49059 significantly decreased platelet aggregation and number of apoptotic cells. The neurobehavioral score was promoted significantly after the intervention.
  
  
  
  The results indicate that rapidly increased vasopressin could induce platelet aggregation and contribute to early brain injury after SAH.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Zun-Wei
authors.initials: ZW
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China; Department of Orthopedic trauma, Honghui Hospital, Xi'an Jiaotong University Health Science Center, No. 76 Nanguo Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Bin-Fei
authors.initials: BF
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Yong-Lin
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, Shaanxi 710061, People׳s Republic of China.
authors.forename: Jin-Ning
authors.initials: JN
authors.lastname: Song
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D006727:Hormone Antagonists / Q000494:pharmacology; D007150:Immunohistochemistry; D007211:Indoles / Q000494:pharmacology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*; D011759:Pyrrolidines / Q000494:pharmacology; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000097:blood / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014667:Vasopressins / Q000378:metabolism*
chemical_list: D006727:Hormone Antagonists; D007211:Indoles; D018696:Neuroprotective Agents; D011759:Pyrrolidines; D014667:Vasopressins; C082134:relcovaptan
keywords: Early brain injury; Microthrombosis; Platelet aggregation; Subarachnoid hemorrhage; Vasopressin