title: The effect of chronic tianeptine administration on the brain mitochondria: direct links with an animal model of depression.
abstract: A growing body of evidence has focused on the impact of mitochondrial disturbances in the development of depression, but little data exist regarding the effects of chronic administration of antidepressant drugs on the brain's mitochondrial protein profile. The aim of this study was to investigate the impact of chronic treatment with an atypical antidepressant drug-tianeptine-on the mitochondria-enriched subproteome profile in the hippocampus and the frontal cortex of 3-month-old male rats following a prenatal stress procedure. Rats that were exposed to a prenatal stress procedure displayed depressive- and anxiety-like disturbances based on the elevated plus-maze and Porsolt tests. Moreover, two-dimensional electrophoresis coupled with mass spectrometry showed structure-dependent mitoproteome changes in brains of prenatally stressed rats after chronic tianeptine administration. A component of 2-oxoglutarate and succinate flavoprotein subunit dehydrogenases, isocitrate subunit alpha, was upregulated in the hippocampus. In the frontal cortex, there was a striking increase in the expression of glutamate dehydrogenase and cytochrome bc1 complex subunit 2. These findings suggest that mitochondria are underappreciated targets for therapeutic interventions, and mitochondrial function may be crucial for the effective treatment of stress-related diseases.
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna St, 31-343, Kraków, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Głombik
authors.affiliation: Chair of Pharmacology, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531, Kraków, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Stachowicz
authors.affiliation: Chair of Pharmacology, Jagiellonian University Medical College, 16 Grzegórzecka Street, 31-531, Kraków, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Olszanecki
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna St, 31-343, Kraków, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Ślusarczyk
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna St, 31-343, Kraków, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Trojan
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna St, 31-343, Kraków, Poland.
authors.forename: Władysław
authors.initials: W
authors.lastname: Lasoń
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna St, 31-343, Kraków, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Kubera
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna St, 31-343, Kraków, Poland.
authors.forename: Bogusława
authors.initials: B
authors.lastname: Budziszewska
authors.affiliation: Physiopathogie des Maladies Psychiatriques, INSERM UMR_S 894, Centre de Psychiatrie et Neurosciences, 2ter rue d'Alesia, 75014, Paris, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Spedding
authors.affiliation: Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, 12 Smętna St, 31-343, Kraków, Poland. basta@if-pan.krakow.pl.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Basta-Kaim
mesh_terms: D000818:Animals; D001007:Anxiety / Q000150:complications / Q000188:drug therapy; D001522:Behavior, Animal; D001921:Brain / Q000378:metabolism*; D003863:Depression / Q000150:complications / Q000188:drug therapy* / Q000473:pathology*; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006624:Hippocampus / Q000473:pathology; D007099:Imipramine / Q000627:therapeutic use; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000188:drug therapy / Q000473:pathology; D020543:Proteome / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013841:Thiazepines / Q000008:administration & dosage* / Q000627:therapeutic use*
chemical_list: D024101:Mitochondrial Proteins; D020543:Proteome; D013841:Thiazepines; C050504:tianeptine; D007099:Imipramine
keywords: Frontal cortex; Hippocampus; Mitochondria; Prenatal stress; Tianeptine