title: [DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION].
abstract: OBJECTIVE
  Detection-and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR).
  
  
  METHODS
  VEE, Sindbis, West Nile, Japanese and tick-borne encephalitis viruses were studied. Cell culture of chicken fibroblasts, outbred mice and rats, Javanese macaques were used in the experiments. Biological activity determination of the running culture of causative agents used in the experiments was carried out by negative colony method in monolayer cell culture under agar coating. and using intra-cerebral infection of mice. Reagent kits developed in the 48th Central Research Institute and Institute of Analytical Instrument Engineering were used during execution of experiments of VEE virus RNA detection by RT-PCR and rRT-PCR.
  
  
  RESULTS
  VEE virus was detected in biological samples by various methods. Data from RT-PCR and rRT-PCR are in accordance with the results of virus detection in samples using sensitive animals.
  
  
  CONCLUSIONS
  Use of molecular-diagnostics methods for detection in biological samples of a causative agent of a dangerous infectious disease is important for procuring biological safety of Russian Federation.
authors.forename: A A
authors.initials: AA
authors.lastname: Petrov
authors.forename: N S
authors.initials: NS
authors.lastname: Pyshnaya
authors.forename: V N
authors.initials: VN
authors.lastname: Lebedev
authors.forename: V S
authors.initials: VS
authors.lastname: Kulish
authors.forename: L F
authors.initials: LF
authors.lastname: Stovba
authors.forename: A V
authors.initials: AV
authors.lastname: Kazantsev
authors.forename: S V
authors.initials: SV
authors.lastname: Borisevich
mesh_terms: D018354:Alphavirus Infections / Q000175:diagnosis / Q000821:virology; D000818:Animals; D021461:Animals, Outbred Strains; D002645:Chickens; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000302:isolation & purification; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics* / Q000302:isolation & purification; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics* / Q000302:isolation & purification; D004672:Encephalitis, Japanese / Q000175:diagnosis / Q000821:virology; D004675:Encephalitis, Tick-Borne / Q000175:diagnosis / Q000821:virology; D004685:Encephalomyelitis, Venezuelan Equine / Q000175:diagnosis / Q000821:virology; D005347:Fibroblasts / Q000473:pathology / Q000821:virology; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011933:Reagent Kits, Diagnostic / Q000592:standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012845:Sindbis Virus / Q000235:genetics* / Q000302:isolation & purification; D014901:West Nile Fever / Q000175:diagnosis / Q000821:virology; D014902:West Nile virus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic