title: 5-Methylcytosine-Rich Heterochromatin in the Indian Muntjac.
abstract: Two 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the 'neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in the centromeric region of the free autosome 3 after denaturing the chromosomal DNA for 3 h or longer. The structure and possible function of the major hypermethylated region as barrier against spreading of the X-inactivation process into the autosome 3 is discussed.
authors.affiliation: Department of Human Genetics, University of Wx00FC;rzburg, Wx00FC;rzburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schmid
authors.forename: Claus
authors.initials: C
authors.lastname: Steinlein
authors.forename: Christian
authors.initials: C
authors.lastname: Lomb
authors.forename: Karl
authors.initials: K
authors.lastname: Sperling
authors.forename: Heidemarie
authors.initials: H
authors.lastname: Neitzel
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D006570:Heterochromatin / Q000737:chemistry*; D008297:Male; D018668:Muntjacs / Q000235:genetics*
chemical_list: D006570:Heterochromatin; D044503:5-Methylcytosine