title: Isolation and characterization of 13 microsatellites for the rare endemic shrub Tetratheca erubescens (Elaeocarpaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the rare Tetratheca erubescens (Elaeocarpaceae) to assess genetic diversity and spatial structuring.
  
  
  RESULTS
  We generated ca. 2.7 million sequence reads using a Personal Genome Machine (PGM) semiconductor sequencer. Using the QDD pipeline, we designed primers for >12,000 sequences with PCR product lengths of 80-480 bp. From these, 30 primer pairs were selected and screened using PCR, from which 11 loci were found to be polymorphic and amplified reliably. For a sample of 95 plants from three populations, the number of alleles observed for these 11 loci ranged from two to seven and expected heterozygosity ranged from 0.06 to 0.72. No consistent evidence for null alleles or departure from Hardy-Weinberg equilibrium was found for any of the 11 loci.
  
  
  CONCLUSIONS
  These markers will enable the quantification of genetic impact of proposed mining activities on the narrow endemic T. erubescens.
authors.affiliation: Kings Park and Botanic Garden, Botanic Garden and Parks Authority, Fraser Avenue, West Perth, 6005 Western Australia, Australia; School of Plant Biology, The University of Western Australia, Nedlands, 6009 Western Australia, Australia.
authors.forename: Janet M
authors.initials: JM
authors.lastname: Anthony
authors.affiliation: School of Pathology and Laboratory Medicine, Lotterywest State Biomedical Facility Genomics, University of Western Australia, Crawley, Western Australia 6009, Australia.
authors.forename: Richard J N
authors.initials: RJ
authors.lastname: Allcock
authors.affiliation: Kings Park and Botanic Garden, Botanic Garden and Parks Authority, Fraser Avenue, West Perth, 6005 Western Australia, Australia; School of Plant Biology, The University of Western Australia, Nedlands, 6009 Western Australia, Australia.
authors.forename: Siegfried L
authors.initials: SL
authors.lastname: Krauss
keywords: Elaeocarpaceae; Personal Genome Machine; Tetratheca erubescens; microsatellite primers; shotgun sequencing