title: Improving Functional Annotation in the DRE-TIM Metallolyase Superfamily through Identification of Active Site Fingerprints.
abstract: Within the DRE-TIM metallolyase superfamily, members of the Claisen-like condensation (CC-like) subgroup catalyze C-C bond-forming reactions between various α-ketoacids and acetyl-coenzyme A. These reactions are important in the metabolic pathways of many bacterial pathogens and serve as engineering scaffolds for the production of long-chain alcohol biofuels. To improve functional annotation and identify sequences that might use novel substrates in the CC-like subgroup, a combination of structural modeling and multiple-sequence alignments identified active site residues on the third, fourth, and fifth β-strands of the TIM-barrel catalytic domain that are differentially conserved within the substrate-diverse enzyme families. Using α-isopropylmalate synthase and citramalate synthase from Methanococcus jannaschii (MjIPMS and MjCMS), site-directed mutagenesis was used to test the role of each identified position in substrate selectivity. Kinetic data suggest that residues at the β3-5 and β4-7 positions play a significant role in the selection of α-ketoisovalerate over pyruvate in MjIPMS. However, complementary substitutions in MjCMS fail to alter substrate specificity, suggesting residues in these positions do not contribute to substrate selectivity in this enzyme. Analysis of the kinetic data with respect to a protein similarity network for the CC-like subgroup suggests that evolutionarily distinct forms of IPMS utilize residues at the β3-5 and β4-7 positions to affect substrate selectivity while the different versions of CMS use unique architectures. Importantly, mapping the identities of residues at the β3-5 and β4-7 positions onto the protein similarity network allows for rapid annotation of probable IPMS enzymes as well as several outlier sequences that may represent novel functions in the subgroup.
authors.affiliation: Department of Chemistry, The University of Alabama , 250 Hackberry Lane, Tuscaloosa, Alabama 35487, United States.
authors.forename: Garima
authors.initials: G
authors.lastname: Kumar
authors.affiliation: Department of Chemistry, The University of Alabama , 250 Hackberry Lane, Tuscaloosa, Alabama 35487, United States.
authors.forename: Jordyn L
authors.initials: JL
authors.lastname: Johnson
authors.affiliation: Department of Chemistry, The University of Alabama , 250 Hackberry Lane, Tuscaloosa, Alabama 35487, United States.
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Frantom
mesh_terms: D015079:2-Isopropylmalate Synthase / Q000737:chemistry* / Q000502:physiology*; D020134:Catalytic Domain / Q000502:physiology; D063747:Methanocaldococcus / Q000201:enzymology*; D010449:Peptide Mapping / Q000379:methods*; D017433:Protein Structure, Secondary
chemical_list: D015079:2-Isopropylmalate Synthase