title: Long non-coding RNA GAS5 inhibited hepatitis C virus replication by binding viral NS3 protein.
abstract: HCV infection has a complex and dynamic process which involves a large number of viral and host factors. Long non-coding RNA GAS5 inhibits liver fibrosis and liver tumor migration and invasion. However, the contribution of GAS5 on HCV infection remains unknown. In this study, GAS5 was gradually upregulated during HCV infection in Huh7 cells. In addition, GAS5 attenuated virus replication with its 5' end sequences, as confirmed by different GAS5 truncations. Moreover, this 5' end sequences showed RNA-protein interaction with HCV NS3 protein that could act as a decoy to inhibit its functions, which contributed to the suppression of HCV replication. Finally, the innate immune responses remained low in HCV infected Huh7 cells, ruling out the possibility of GAS5 to modulate innate immunity. Thus, HCV stimulated endogenous GAS5 can suppress HCV infection by acting as HCV NS3 protein decoy, providing a potential role of GAS5 as a diagnostic or therapeutic target.
authors.affiliation: Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800th Xiangyin Road, Shanghai 200433, PR China.
authors.forename: Xijing
authors.initials: X
authors.lastname: Qian
authors.affiliation: Department of Orthopedics, Changzheng Hospital Affiliated to Second Military Medical University, 415th Feng Yang Road, Shanghai 200003, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800th Xiangyin Road, Shanghai 200433, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, 800th Xiangyin Road, Shanghai 200433, PR China. Electronic address: qizt@smmu.edu.cn.
authors.forename: Zhongtian
authors.initials: Z
authors.lastname: Qi
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D045744:Cell Line, Tumor; D003412:Cricetulus; D005786:Gene Expression Regulation; D016174:Hepacivirus / Q000235:genetics* / Q000254:growth & development; D022781:Hepatocytes / Q000276:immunology* / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D061251:Primary Cell Culture; D011485:Protein Binding; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000276:immunology; D012367:RNA, Viral / Q000235:genetics* / Q000276:immunology; D015398:Signal Transduction; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology; D014779:Virus Replication / Q000235:genetics* / Q000276:immunology; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D062085:RNA, Long Noncoding; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C561567:GAS5 long non-coding RNA, human; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: GAS5; Hepatitis C virus; Long non-coding RNA; NS3