title: Strain-Specific Survival of Salmonella enterica in Peanut Oil, Peanut Shell, and Chia Seeds.
abstract: In North America, outbreaks of Salmonella have been linked to low-water activity (aw) foods, such as nuts and seeds. These outbreaks have implicated an assortment of Salmonella serotypes. Some Salmonella serotypes (e.g., Enteritidis and Typhimurium) cause high proportions of salmonellosis. Nevertheless, there has recently been an emergence of uncommon Salmonella serotypes and strains (e.g., Tennessee, Hartford, and Thompson) in low-aw foods. The aim of this study was to evaluate the survival characteristics of Salmonella serotypes Enteritidis, Typhimurium, Tennessee, Hartford, and Thompson in three low-aw food ingredients with varying aw: peanut oil (aw = 0.521 ± 0.003), peanut shell (aw = 0.321 ± 0.20), and chia seeds (aw = 0.585 ± 0.003). The survival of individual Salmonella strains on each food matrix was monitored for a maximum of 150 days by spreading the bacterial cells onto Luria-Bertani and/or xylose lysine deoxycholate agar. Overall, Salmonella survived for the longest periods of time in peanut oil (96 ± 8 days), followed by chia seeds (94 ± 46 days). The survival period was substantially reduced on the surface of peanut shell (42 ± 49 h), although PCR after 70 days of incubation revealed the presence of Salmonella cells. In addition, Salmonella exhibited a strain-specific response in the three low-aw foods tested. Salmonella Hartford was identified as highly persistent in all low-aw food matrices, whereas Salmonella Typhimurium was the least persistent. The current research emphasizes the adaptable nature of Salmonella to low-aw food ingredients. This may pose additional problems owing to the downstream production of various end products. Additionally, unique survival characteristics among Salmonella strains highlight the need for tailored mitigation strategies regarding high-risk Salmonella strains in the food industry.
authors.affiliation: Food, Nutrition and Health, Faculty of Land and Food Systems, The University of British Columbia, 2205 East Mall, Vancouver, British Columbia, Canada V6T 1Z4.
authors.forename: Karen
authors.initials: K
authors.lastname: Fong
authors.affiliation: Food, Nutrition and Health, Faculty of Land and Food Systems, The University of British Columbia, 2205 East Mall, Vancouver, British Columbia, Canada V6T 1Z4. siyun.wang@ubc.ca.
authors.forename: Siyun
authors.initials: S
authors.lastname: Wang
mesh_terms: D010367:Arachis / Q000382:microbiology*; D015169:Colony Count, Microbial; D005506:Food Contamination; D005516:Food Microbiology; D050296:Microbial Viability; D009656:North America; D000074241:Peanut Oil; D010938:Plant Oils / Q000737:chemistry*; D019779:Salmonella enterica / Q000145:classification / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000302:isolation & purification; D027544:Salvia / Q000382:microbiology*; D012639:Seeds / Q000382:microbiology*
chemical_list: D000074241:Peanut Oil; D010938:Plant Oils