title: Comparative Analysis of HIV-1 and Murine Leukemia Virus Three-Dimensional Nuclear Distributions.
abstract: Recent advances in fluorescence microscopy allow three-dimensional analysis of HIV-1 preintegration complexes in the nuclei of infected cells. To extend this investigation to gammaretroviruses, we engineered a fluorescent Moloney murine leukemia virus (MLV) system consisting of MLV-integrase fused to enhanced green fluorescent protein (MLV-IN-EGFP). A comparative analysis of lentiviral (HIV-1) and gammaretroviral (MLV) fluorescent complexes in the nuclei of infected cells revealed their different spatial distributions. This research tool has the potential to achieve new insight into the nuclear biology of these retroviruses.
authors.affiliation: Laboratory of Biology, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Quercioli
authors.affiliation: Laboratory of Biology, Scuola Normale Superiore, Pisa, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Di Primio
authors.affiliation: Laboratory of Molecular Virology, University of Trento, Centre for Integrative Biology, Trento, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Casini
authors.affiliation: Department of Microbiology, Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Lubbertus C F
authors.initials: LCF
authors.lastname: Mulder
authors.affiliation: Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.
authors.forename: Lenard S
authors.initials: LS
authors.lastname: Vranckx
authors.affiliation: Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.|Laboratory for Photochemistry and Spectroscopy, Department of Chemistry, KU Leuven, Leuven, Belgium.
authors.forename: Doortje
authors.initials: D
authors.lastname: Borrenberghs
authors.affiliation: Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.
authors.forename: Rik
authors.initials: R
authors.lastname: Gijsbers
authors.affiliation: Laboratory of Molecular Virology and Gene Therapy, KU Leuven, Leuven, Flanders, Belgium.
authors.forename: Zeger
authors.initials: Z
authors.lastname: Debyser
authors.affiliation: Laboratory of Molecular Virology, University of Trento, Centre for Integrative Biology, Trento, Italy anna.cereseto@unitn.it.
authors.forename: Anna
authors.initials: A
authors.lastname: Cereseto
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure* / Q000821:virology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D019426:Integrases / Q000235:genetics; D051379:Mice; D008856:Microscopy, Fluorescence; D008979:Moloney murine leukemia virus / Q000502:physiology* / Q000648:ultrastructure; D016662:Virus Integration
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D019426:Integrases