title: Dual-Reactable Fluorescent Probes for Highly Selective and Sensitive Detection of Biological H2 S.
abstract: Hydrogen sulfide (H2 S) is an important endogenous signaling molecule with a variety of biological functions. Development of fluorescent probes for highly selective and sensitive detection of H2 S is necessary. We show here that dual-reactable fluorescent H2 S probes could react with higher selectivity than single-reactable probes. One of the dual-reactable probes gives more than 4000-fold turn-on response when reacting with H2 S, the largest response among fluorescent H2 S probes reported thus far. In addition, the probe could be used for high-throughput enzymatic assays and for the detection of Cys-induced H2 S in cells and in zebrafish. These dual-reactable probes hold potential for highly selective and sensitive detection of H2 S in biological systems.
authors.affiliation: Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wei
authors.affiliation: Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China.
authors.forename: Runyu
authors.initials: R
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029, P. R. China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China.
authors.forename: Guoce
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China.
authors.forename: Qiang-Zhe
authors.initials: QZ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China.
authors.forename: Lu-Yuan
authors.initials: LY
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029, P. R. China. yilong@mail.buct.edu.cn.
authors.forename: Long
authors.initials: L
authors.lastname: Yi
authors.affiliation: Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China. zhenxi@nankai.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xi
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism; D015394:Molecular Structure; D015027:Zebrafish
chemical_list: D005456:Fluorescent Dyes; D006862:Hydrogen Sulfide
keywords: dual-quenching; dual-reactable; fluorescent imaging; hydrogen sulfide; imaging agents