title: Expression and function of HSP110 family in mouse testis after vasectomy.
abstract: HSP110 functions to protect cells, tissues, and organs from noxious conditions. Vasectomy induces apoptosis in the testis; however, little is known about the reason leading to this outcome. The aim of the present study was to evaluate the expression and function of HSP110 in mouse testis after vasectomy. Following bilateral vasectomy, we used fluorescent Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to detect apoptosis, Western blotting and immunohistochemistry to examine HSP110 expression and localization. Serum antisperm antibody (AsAb) and testosterone were measured by Enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay, respectively. Expression of endoplasmic reticulum stress (ERS) sensors and downstream signaling components was measured by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), and the phosphorylation of eIF2α and JNK was detected by Western blotting. Vasectomy induced morphologic changes, increased apoptosis in the testis, increased serum AsAb, and decreased testosterone levels. After vasectomy, ORP150 mRNA level was increased first and then decreased, Bcl-2 was decreased, and the expression of HSPA4l, GRP78, GADD153, PERK, ATF6, IRE-1, XBP-1s, Bax, Bak, and caspases and the phosphorylation of eIF2α and JNK were increased. We present that an ER stress-mediated pathway is activated and involved in apoptosis in the testis after vasectomy. HSPA4l and ORP150 may play important roles in maintaining the normal structure and function of testis.
authors.affiliation: Department of Medicine, Yantai University, Yantai 264005, China.
authors.forename: Zhen-Ning
authors.initials: ZN
authors.lastname: Du
authors.affiliation: Department of Medicine, Yantai University, Yantai 264005, China.|Pharmacy Department of Fuyang People's Hospital, Fuyang 236001, China.
authors.forename: Cheng-Ting
authors.initials: CT
authors.lastname: Rong
authors.affiliation: Department of Medicine, Yantai University, Yantai 264005, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Hui
authors.affiliation: Yantai Yuhuangding Hospital, Yantai 264000, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Yantai Yuhuangding Hospital, Yantai 264000, China.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Jin
authors.affiliation: Yantai Yuhuangding Hospital, Yantai 264000, China.
authors.forename: Shi-Jia
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Department of Medicine, Yantai University, Yantai 264005, China.|Yantai Yuhuangding Hospital, Yantai 264000, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Department of Medicine, Yantai University, Yantai 264005, China.|Yantai Yuhuangding Hospital, Yantai 264000, China.|Key Laboratory of Male Reproductive Health, National Health and Family Planning Commission, Beijing 100081, China.
authors.forename: Jian-Yuan
authors.initials: JY
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D050957:HSP110 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011863:Radioimmunoassay; D013094:Spermatozoa / Q000276:immunology; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D014659:Vasectomy
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D050957:HSP110 Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D013739:Testosterone