title: Cytokine, chemokine, and growth factor profile of platelet-rich plasma.
abstract: During wound healing, biologically active molecules are released from platelets. The rationale of using platelet-rich plasma (PRP) relies on the concentration of bioactive molecules and subsequent delivery to healing sites. These bioactive molecules have been seldom simultaneously quantified within the same PRP preparation. In the present study, the flexible Bio-Plex system was employed to assess the concentration of a large range of cytokines, chemokines, and growth factors in 16 healthy volunteers so as to determine whether significant baseline differences may be found. Besides IL-1b, IL-1ra, IL-4, IL-6, IL-8, IL-12, IL-13, IL-17, INF-γ, TNF-α, MCP-1, MIP-1a, RANTES, bFGF, PDGF, and VEGF that were already quantified elsewhere, the authors reported also on the presence of IL-2, IL-5, IL-7, IL-9, IL-10, IL-15 G-CSF, GM-CSF, Eotaxin, CXCL10 chemokine (IP-10), and MIP 1b. Among the most interesting results, it is convenient to mention the high concentrations of the HIV-suppressive and inflammatory cytokine RANTES and a statistically significant difference between males and females in the content of PDGF-BB. These data are consistent with previous reports pointing out that gender, diet, and test system affect the results of platelet function in healthy subjects, but seem contradictory when compared to other quantification assays in serum and plasma. The inconsistencies affecting the experimental results found in literature, along with the variability found in the content of bioactive molecules, urge further research, hopefully in form of randomized controlled clinical trials, in order to find definitive evidence of the efficacy of PRP treatment in various pathologic and regenerative conditions.
authors.affiliation: a CIR Dental School, Department of Surgical Sciences , University of Turin , Turin , Italy.
authors.forename: F
authors.initials: F
authors.lastname: Mussano
authors.affiliation: a CIR Dental School, Department of Surgical Sciences , University of Turin , Turin , Italy.|b Department of Life Sciences and Systems Biology , University of Turin , Turin , Italy.
authors.forename: T
authors.initials: T
authors.lastname: Genova
authors.affiliation: b Department of Life Sciences and Systems Biology , University of Turin , Turin , Italy.|c Centre for Nanostructured Interfaces and Surfaces (NIS) , University of Turin , Turin , Italy.
authors.forename: L
authors.initials: L
authors.lastname: Munaron
authors.affiliation: d Molecular Biotechnology Center , University of Turin , Turin , Italy.
authors.forename: S
authors.initials: S
authors.lastname: Petrillo
authors.affiliation: a CIR Dental School, Department of Surgical Sciences , University of Turin , Turin , Italy.
authors.forename: F
authors.initials: F
authors.lastname: Erovigni
authors.affiliation: a CIR Dental School, Department of Surgical Sciences , University of Turin , Turin , Italy.
authors.forename: S
authors.initials: S
authors.lastname: Carossa
mesh_terms: D000328:Adult; D001772:Blood Cell Count; D018925:Chemokines / Q000097:blood; D016207:Cytokines / Q000097:blood; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000097:blood; D008297:Male; D053657:Platelet-Rich Plasma / Q000737:chemistry; D055815:Young Adult
chemical_list: D018925:Chemokines; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins
keywords: Chemokines; cytokines; growth factors; platelet-rich plasma