title: Lipoxygenase inhibitors from the latex of Calotropis Procera.
abstract: A radical-scavenging, guided phytochemical study of the latex of Calotropis Procera afforded five lignans (1-5), including a new one (4). The structural determination was accomplished using 1D- and 2D-NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and correlation with known compounds. Among the isolated compounds, acylated lignans (3-5) showed stronger antioxidant activity than non-acylated derivatives (1,2). Anti-inflammatory activity was evaluated by determining the inhibitory potential against 5- and 15-lipoxygenase enzymes. The highest anti-inflammatory activity was observed in compound 4, with IC50svalues of 7.6 µM and 2.7 µM against 5-LOX and 15-LOX, respectively.
authors.affiliation: Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.|Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
authors.forename: Wael M
authors.initials: WM
authors.lastname: Abdel-Mageed
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt.|Department of Biology, Faculty of Science and Art, Samtah, Jazan University, Jazan, Saudi Arabia.
authors.forename: Nadia H
authors.initials: NH
authors.lastname: Mohamed
authors.affiliation: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.|Graduate University of Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Ali A
authors.initials: AA
authors.lastname: El-Gamal
authors.affiliation: Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Omer A
authors.initials: OA
authors.lastname: Basudan
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt.
authors.forename: Mady Ahmed
authors.initials: MA
authors.lastname: Ismail
authors.affiliation: Eskitis Institute, Griffith University, Brisbane, QLD, 4111, Australia.
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
authors.affiliation: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. liuxueting_cn@hotmail.com.
authors.forename: Xueting
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. zhanglixin@im.ac.cn.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt. ashoreit1968@yahoo.com.
authors.forename: Ahmed A M
authors.initials: AA
authors.lastname: Shoreit
keywords: Anti-inflammatory; Antioxidants; Calotropis procera; Lignans; Lipoxygenase inhibitors