title: Expression and partial biochemical characterization of a recombinant serine protease from Bothrops pauloensis snake venom.
abstract: Snake venom serine proteases (SVSPs) are enzymes capable of interfering at several points of hemostasis. Some serine proteases present thrombin-like activity, which makes them targets for the development of therapeutics agents in the treatment of many hemostatic disorders. In this study, a recombinant thrombin-like serine protease, denominated rBpSP-II, was obtained from cDNA of the Bothrops pauloensis venom gland and was characterized enzymatically and biochemically. The enzyme rBpSP-II showed clotting activity on bovine plasma and proteolytic activity on fibrinogen, cleaving exclusively the Aα chain. The evaluation of rBpSP-II activity on chromogenic substrates demonstrated thrombin-like activity of the enzyme due to its capacity to hydrolyze the thrombin substrate. These characteristics make rBpSP-II an attractive molecule for additional studies. Further research is needed to verify whether rBpSP-II can serve as a template for the synthesis of therapeutic agents to treat hemostatic disorders.
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
authors.forename: Thais F
authors.initials: TF
authors.lastname: Isabel
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
authors.forename: Guilherme Nunes Moreira
authors.initials: GN
authors.lastname: Costa
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
authors.forename: Isabela B
authors.initials: IB
authors.lastname: Pacheco
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
authors.forename: Luana G
authors.initials: LG
authors.lastname: Barbosa
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
authors.forename: Célio D
authors.initials: CD
authors.lastname: Santos-Junior
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil.
authors.forename: Fernando P P
authors.initials: FP
authors.lastname: Fonseca
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Física e Química, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Johara
authors.initials: J
authors.lastname: Boldrini França
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil.
authors.forename: Flávio
authors.initials: F
authors.lastname: Henrique-Silva
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
authors.forename: Kelly A G
authors.initials: KA
authors.lastname: Yoneyama
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil; INCT, Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica, Belo Horizonte, MG, Brazil. Electronic address: rsrodrigues@ingeb.ufu.br.
authors.forename: Renata S
authors.initials: RS
authors.lastname: Rodrigues
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil; INCT, Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica, Belo Horizonte, MG, Brazil.
authors.forename: Veridiana de Melo
authors.initials: Vde M
authors.lastname: Rodrigues
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D017837:Bothrops; D002417:Cattle; D005340:Fibrinogen / Q000737:chemistry; D020141:Hemostatic Disorders / Q000188:drug therapy; D006868:Hydrolysis / Q000187:drug effects; D011994:Recombinant Proteins / Q000737:chemistry; D057057:Serine Proteases / Q000737:chemistry*; D012910:Snake Venoms / Q000201:enzymology*; D013917:Thrombin / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D012910:Snake Venoms; D005340:Fibrinogen; D057057:Serine Proteases; D013917:Thrombin
keywords: Bothrops pauloensis; Heterologous expression; Snake venom; Thrombin-like; serine protease