title: Sororin loads to the synaptonemal complex central region independently of meiotic cohesin complexes.
abstract: The distribution and regulation of the cohesin complexes have been extensively studied during mitosis. However, the dynamics of their different regulators in vertebrate meiosis is largely unknown. In this work, we have analyzed the distribution of the regulatory factor Sororin during male mouse meiosis. Sororin is detected at the central region of the synaptonemal complex during prophase I, in contrast with the previously reported localization of other cohesin components in the lateral elements. This localization of Sororin depends on the transverse filaments protein SYCP1, but not on meiosis-specific cohesin subunits REC8 and SMC1β. By late prophase I, Sororin accumulates at centromeres and remains there up to anaphase II The phosphatase activity of PP2A seems to be required for this accumulation. We hypothesize that Sororin function at the central region of the synaptonemal complex could be independent on meiotic cohesin complexes. In addition, we suggest that Sororin participates in the regulation of centromeric cohesion during meiosis in collaboration with SGO2-PP2A.
authors.affiliation: Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain rocio.gomez@uam.es jose.suja@uam.es.
authors.forename: Rocío
authors.initials: R
authors.lastname: Gómez
authors.affiliation: Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca) Campus Miguel de Unamuno, Salamanca, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Felipe-Medina
authors.affiliation: Chromosome Dynamics Group, Centro Nacional de Investigaciones Oncológicas CNIO, Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Ruiz-Torres
authors.affiliation: Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Inés
authors.initials: I
authors.lastname: Berenguer
authors.affiliation: Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Viera
authors.affiliation: Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Pérez
authors.affiliation: Departamento de Biología Celular y Molecular, Centro de Investigaciones Biológicas CSIC, Madrid, Spain.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Barbero
authors.affiliation: Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca) Campus Miguel de Unamuno, Salamanca, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Llano
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: Department of Cell and Developmental Biology, Biocenter University of Würzburg, Würzburg, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Alsheimer
authors.affiliation: Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca) Campus Miguel de Unamuno, Salamanca, Spain.
authors.forename: Alberto M
authors.initials: AM
authors.lastname: Pendás
authors.affiliation: Chromosome Dynamics Group, Centro Nacional de Investigaciones Oncológicas CNIO, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Losada
authors.affiliation: Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain rocio.gomez@uam.es jose.suja@uam.es.
authors.forename: José A
authors.initials: JA
authors.lastname: Suja
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D002503:Centromere; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D018345:Mice, Knockout; D013090:Spermatocytes / Q000378:metabolism; D013573:Synaptonemal Complex; D000097722:Cohesins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins; C500695:CDCA5 protein, human
keywords: Sororin; centromere; cohesin complex; meiosis; synaptonemal complex