title: De Novo Transcriptome Analysis of Medicinally Important Plantago ovata Using RNA-Seq.
abstract: Plantago ovata is an economically and medicinally important plant of the family Plantaginaceae. It is used extensively for the production of seed husk for its application in pharmaceutical, food and cosmetic industries. In the present study, the transcriptome of P. ovata ovary was sequenced using Illumina Genome Analyzer platform to characterize the mucilage biosynthesis pathway in the plant. De novo assembly was carried out using Oases followed by velvet. A total of 46,955 non-redundant transcripts (≥100 bp) using ~29 million high-quality paired end reads were generated. Functional categorization of these transcripts revealed the presence of several genes involved in various biological processes like metabolic pathways, mucilage biosynthesis, biosynthesis of secondary metabolites and antioxidants. In addition, simple sequence-repeat motifs, non-coding RNAs and transcription factors were also identified. Expression profiling of some genes involved in mucilage biosynthetic pathway was performed in different tissues of P. ovata using Real time PCR analysis. The study has resulted in a valuable resource for further studies on gene expression, genomics and functional genomics in P. ovata.
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu & Kashmir, 180006, India.
authors.forename: Shivanjali
authors.initials: S
authors.lastname: Kotwal
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu & Kashmir, 180006, India.
authors.forename: Sanjana
authors.initials: S
authors.lastname: Kaul
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu & Kashmir, 180006, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Sharma
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu & Kashmir, 180006, India.
authors.forename: Mehak
authors.initials: M
authors.lastname: Gupta
authors.affiliation: National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Rama
authors.initials: R
authors.lastname: Shankar
authors.affiliation: National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, 110067, India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Jain
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, Jammu & Kashmir, 180006, India.
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Dhar
mesh_terms: D001482:Base Composition / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D053858:Metabolic Networks and Pathways / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D058977:Molecular Sequence Annotation; D059372:Nucleotide Motifs / Q000235:genetics; D010943:Plantago / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*; D020543:Proteome / Q000378:metabolism; D011786:Quality Control; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012098:Reproduction; D017423:Sequence Analysis, RNA / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D012984:Software; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D020543:Proteome; D012333:RNA, Messenger; D022661:RNA, Untranslated; D014157:Transcription Factors