title: [HPLC Specific Fingerprint of Alcohol Extract of Andrographis paniculata].
abstract: OBJECTIVE
  To establish the specific fingerprint of alcohol extract of Andrographis paniculata by HPLC.
  
  
  METHODS
  The analysis was performed on Cosmosil 5C18 -MS-II (250 mm x 4. 6 mm, 5 µm) column, with gradient phase consisting of acetonitrile and water at the flow rate of 1. 0 mL/min. The UV detection wavelength was set at 225 nm, and column temperature was 30 °C.
  
  
  RESULTS
  The specific fingerprint chromatogram was established and seven common peaks were identified by comparison with the reference standards and LC-MS. The relative retention times were 1. 00 (No. 1, andrographolide), 1. 04 (No. 2, deoxyandrographoside), 1. 07 (No. 3, isoandrographolide), 1. 10 (No. 4, 14-deoxy-11, 12-didehydroandrographoside), 1. 50 (No. 5, neoandrographolide), 1. 75 ( No. 6, deoxyandrographolide) and 1. 79 (No. 7, dehydroandrographolide).
  
  
  CONCLUSIONS
  The method is simple and reproducible with high precision, which can provide the basis for the quality control and evaluation of alcohol extract of Andrographis paniculata and its preparations.
authors.forename: Fei-fei
authors.initials: FF
authors.lastname: Liu
authors.forename: Chun-lin
authors.initials: CL
authors.lastname: Fan
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Huang
authors.forename: Gui-yang
authors.initials: GY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Wen-cai
authors.initials: WC
authors.lastname: Ye
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D000431:Ethanol; D005960:Glucosides; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013764:Tetrahydronaphthalenes
chemical_list: D004224:Diterpenes; D005960:Glucosides; D010936:Plant Extracts; D013764:Tetrahydronaphthalenes; D000431:Ethanol; C030419:andrographolide; C478098:dehydroandrographolide; C082497:neoandrographolide