title: Identification and detoxification of glycolaldehyde, an unattended bioethanol fermentation inhibitor.
abstract: Although there have been approximately 60 chemical compounds identified as potent fermentation inhibitors in lignocellulose hydrolysate, our research group recently discovered glycolaldehyde as a key fermentation inhibitor during second generation biofuel production. Accordingly, we have developed a yeast S. cerevisiae strain exhibiting tolerance to glycolaldehyde. During this glycolaldehyde study, we established novel approaches for rational engineering of inhibitor-tolerant S. cerevisiae strains, including engineering redox cofactors and engineering the SUMOylation pathway. These new technical dimensions provide a novel platform for engineering S. cerevisiae strains to overcome one of the key barriers for industrialization of lignocellulosic ethanol production. As such, this review discusses novel biochemical insight of glycolaldehyde in the context of the biofuel industry.
authors.affiliation: a Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign , Urbana , IL , USA.|b Department of Biochemistry and Applied Biosciences United Graduate School of Agricultural Sciences , Kagoshima University , Kagoshima , Japan , and.
authors.forename: Lahiru N
authors.initials: LN
authors.lastname: Jayakody
authors.affiliation: c Department of Environmental Sciences Faculty of Agriculture , Saga University , Saga , Japan.
authors.forename: Jannatul
authors.initials: J
authors.lastname: Ferdouse
authors.affiliation: c Department of Environmental Sciences Faculty of Agriculture , Saga University , Saga , Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hayashi
authors.affiliation: b Department of Biochemistry and Applied Biosciences United Graduate School of Agricultural Sciences , Kagoshima University , Kagoshima , Japan , and.|c Department of Environmental Sciences Faculty of Agriculture , Saga University , Saga , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitagaki
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000633:toxicity; D056804:Biofuels; D000431:Ethanol / Q000378:metabolism; D019855:Ethylene Glycol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D058207:Sumoylation
chemical_list: D056804:Biofuels; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D019855:Ethylene Glycol; D005978:Glutathione; D000079:Acetaldehyde; C010972:glycolaldehyde
keywords: Bioethanol; S. cerevisiae; SUMOylation; glycolaldehyde; lignocellulose; redox cofactors