title: Immunohistochemical evaluation of hedgehog signalling in epithelial/mesenchymal interactions in squamous cell carcinoma transformation: a pilot study.
abstract: Precancerous lesions have been studied because of their carcinogenic potential and their association with squamous cell carcinoma (SCC) has been reported. In the tumour microenvironment, the processes of angiogenesis and tissue remodelling are regulated by a family of proteins (Hedgehog) described as being able to modulate epithelial/mesenchymal interactions. The objective of this study was to perform a comparative study of precancerous lesions and SCCs by immunohistochemistry for the presence of Sonic, Gli2, SMO and Patched proteins, members of the Hedgehog pathway. Sixteen cases diagnosed as actinic cheilitis associated with SCC were compared to normal oral mucosa. The sections were subjected to immunohistochemistry and the positively stained cells were counted by morphometric analysis. There was a significant progressive increase in expression of all proteins of the Hedgehog pathway, both in the epithelium and in the connective tissue, when sections of normal mucosa, dysplasia and carcinoma were compared (P < 0.05). Thus, one may suggest that the Hedgehog pathway in tumour transformation influences SCC, and more studies should be conducted to expand the understanding of the role of these proteins in neoplastic transformation.
authors.affiliation: Laboratory of Experimental Pathology, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Gonzalez
authors.affiliation: Basic Science, Bahiana Schoool of Medicine and Public Health, Salvador, Bahia, Brazil.
authors.forename: Maira
authors.initials: M
authors.lastname: Ferreira
authors.affiliation: Laboratory of Experimental Pathology, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.
authors.forename: Tamires
authors.initials: T
authors.lastname: Ariel
authors.affiliation: Basic Science, Bahiana Schoool of Medicine and Public Health, Salvador, Bahia, Brazil.
authors.forename: Sílvia Regina
authors.initials: SR
authors.lastname: Reis
authors.affiliation: Laboratory of Experimental Pathology, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.
authors.forename: Zilton
authors.initials: Z
authors.lastname: Andrade
authors.affiliation: Basic Science, Bahiana Schoool of Medicine and Public Health, Salvador, Bahia, Brazil.
authors.forename: Alena
authors.initials: A
authors.lastname: Peixoto Medrado
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology*; D006258:Head and Neck Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D053823:Hedgehog Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D010865:Pilot Projects; D015398:Signal Transduction; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D053823:Hedgehog Proteins
keywords: oral cancer; oral dysplasia; oral mucosa