title: Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI-TOF-MS profiling.
abstract: Piscirickettsia salmonis is a pathogenic bacteria known as the aetiological agent of the salmonid rickettsial syndrome and causes a high mortality in farmed salmonid fishes. Detection of P. salmonis in farmed fishes is based mainly on molecular biology and immunohistochemistry techniques. These techniques are in most of the cases expensive and time consuming. In the search of new alternatives to detect the presence of P. salmonis in salmonid fishes, this work proposed the use of MALDI-TOF-MS to compare serum protein profiles from Salmo salar fish, including experimentally infected and non-infected fishes using principal component analysis (PCA). Samples were obtained from a controlled bioassay where S. salar was challenged with P. salmonis in a cohabitation model and classified according to the presence or absence of the bacteria by real time PCR analysis. MALDI spectra of the fish serum samples showed differences in its serum protein composition. These differences were corroborated with PCA analysis. The results demonstrated that the use of both MALDI-TOF-MS and PCA represents a useful tool to discriminate the fish status through the analysis of salmonid serum samples.
authors.affiliation: Instituto de Química de Recursos Naturales, Universidad de Talca, Talca, Chile.
authors.forename: Verónica R
authors.initials: VR
authors.lastname: Olate
authors.affiliation: División Nanobiotecnología, Fraunhofer Chile Research, Talca, Chile.
authors.forename: Fabiane M
authors.initials: FM
authors.lastname: Nachtigall
authors.affiliation: Instituto de Química de Recursos Naturales, Universidad de Talca, Talca, Chile.|División Nanobiotecnología, Fraunhofer Chile Research, Talca, Chile.
authors.forename: Leonardo S
authors.initials: LS
authors.lastname: Santos
authors.affiliation: Instituto de Matemáticas y Física, Universidad de Talca, Talca, Chile.
authors.forename: Alex
authors.initials: A
authors.lastname: Soto
authors.affiliation: Laboratorio de Biotecnología en Acuicultura, Fraunhofer Chile, Bahía Quillaipe, Puerto Montt, Chile.
authors.forename: Macarena
authors.initials: M
authors.lastname: Araya
authors.affiliation: Fundación Chile, Bahía Quillaipe, Puerto Montt, Chile.
authors.forename: Sandra
authors.initials: S
authors.lastname: Oyanedel
authors.affiliation: Fundación Chile, Bahía Quillaipe, Puerto Montt, Chile.
authors.forename: Verónica
authors.initials: V
authors.lastname: Díaz
authors.affiliation: Laboratorio de Biotecnología en Acuicultura, Fraunhofer Chile, Bahía Quillaipe, Puerto Montt, Chile.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Marchant
authors.affiliation: Laboratorio de Biotecnología en Acuicultura, Fraunhofer Chile, Bahía Quillaipe, Puerto Montt, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rios-Momberg
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000097:blood*; D053522:Piscirickettsia / Q000302:isolation & purification*; D044225:Piscirickettsiaceae Infections / Q000097:blood / Q000382:microbiology / Q000662:veterinary; D020317:Salmo salar / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins
keywords: MALDI profiling; Piscirickettsia salmonis detection; Salmo salar; principal component analysis; serum analysis