title: Different spectrophotometric methods applied for the analysis of binary mixture of flucloxacillin and amoxicillin: A comparative study.
abstract: Three different spectrophotometric methods were applied for the quantitative analysis of flucloxacillin and amoxicillin in their binary mixture, namely, ratio subtraction, absorbance subtraction and amplitude modulation. A comparative study was done listing the advantages and the disadvantages of each method. All the methods were validated according to the ICH guidelines and the obtained accuracy, precision and repeatability were found to be within the acceptable limits. The selectivity of the proposed methods was tested using laboratory prepared mixtures and assessed by applying the standard addition technique. So, they can be used for the routine analysis of flucloxacillin and amoxicillin in their binary mixtures.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751, Nasr City, Cairo, Egypt.
authors.forename: Khalid A M
authors.initials: KA
authors.lastname: Attia
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751, Nasr City, Cairo, Egypt.
authors.forename: Mohammed W I
authors.initials: MW
authors.lastname: Nassar
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI), 12582, Al Hadaba Al Wosta, Cairo, Egypt.
authors.forename: Mohamed B
authors.initials: MB
authors.lastname: El-Zeiny
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751, Nasr City, Cairo, Egypt. Electronic address: Ahmedserag777@hotmail.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Serag
mesh_terms: D000658:Amoxicillin / Q000032:analysis*; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002214:Capsules; D004338:Drug Combinations; D005436:Floxacillin / Q000032:analysis*; D057230:Limit of Detection; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D002214:Capsules; D004338:Drug Combinations; D005436:Floxacillin; D000658:Amoxicillin
keywords: Absorbance subtraction; Amoxicillin; Amplitude modulation; Flucloxacillin; Ratio subtraction