title: Separation and Identification of Isomeric Glycans by Selected Accumulation-Trapped Ion Mobility Spectrometry-Electron Activated Dissociation Tandem Mass Spectrometry.
abstract: One of the major challenges in structural characterization of oligosaccharides is the presence of many structural isomers in most naturally occurring glycan mixtures. Although ion mobility spectrometry (IMS) has shown great promise in glycan isomer separation, conventional IMS separation occurs on the millisecond time scale, largely restricting its implementation to fast time-of-flight (TOF) analyzers which often lack the capability to perform electron activated dissociation (ExD) tandem MS analysis and the resolving power needed to resolve isobaric fragments. The recent development of trapped ion mobility spectrometry (TIMS) provides a promising new tool that offers high mobility resolution and compatibility with high-performance Fourier transform ion cyclotron resonance (FTICR) mass spectrometers when operated under the selected accumulation-TIMS (SA-TIMS) mode. Here, we present our initial results on the application of SA-TIMS-ExD-FTICR MS to the separation and identification of glycan linkage isomers.
authors.affiliation: Center for Biomedical Mass Spectrometry, Boston University School of Medicine , Boston, Massachusetts 02118, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Bruker Daltonics , Billerica, Massachusetts 01821, United States.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Ridgeway
authors.affiliation: Center for Biomedical Mass Spectrometry, Boston University School of Medicine , Boston, Massachusetts 02118, United States.
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Glaskin
authors.affiliation: Bruker Daltonics , Billerica, Massachusetts 01821, United States.
authors.forename: Melvin A
authors.initials: MA
authors.lastname: Park
authors.affiliation: Center for Biomedical Mass Spectrometry, Boston University School of Medicine , Boston, Massachusetts 02118, United States.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
authors.affiliation: Center for Biomedical Mass Spectrometry, Boston University School of Medicine , Boston, Massachusetts 02118, United States.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lin
mesh_terms: D005583:Fourier Analysis; D007536:Isomerism; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011134:Polysaccharides