title: The RNA Helicase eIF4A Is Required for Sapovirus Translation.
abstract: The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.
authors.affiliation: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Buk-gu, Gwangju, Republic of Korea.|Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
authors.forename: Myra
authors.initials: M
authors.lastname: Hosmillo
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
authors.forename: Trevor R
authors.initials: TR
authors.lastname: Sweeney
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Chaudhry
authors.affiliation: Department of Life Sciences, Imperial College London, South Kensington, London, United Kingdom.
authors.forename: Eoin
authors.initials: E
authors.lastname: Leen
authors.affiliation: Department of Life Sciences, Imperial College London, South Kensington, London, United Kingdom.
authors.forename: Stephen
authors.initials: S
authors.lastname: Curry
authors.affiliation: Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom ig299@cam.ac.uk choko@chonnam.ac.kr.
authors.forename: Ian
authors.initials: I
authors.lastname: Goodfellow
authors.affiliation: Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Buk-gu, Gwangju, Republic of Korea ig299@cam.ac.uk choko@chonnam.ac.kr.
authors.forename: Kyoung-Oh
authors.initials: KO
authors.lastname: Cho
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics / Q000378:metabolism*; D016679:Genome, Viral; D009154:Mutation; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D029341:Sapovirus / Q000235:genetics* / Q000502:physiology; D013261:Sterols / Q000494:pharmacology; D013552:Swine; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D013261:Sterols; D014764:Viral Proteins; C510125:hippuristanol; D039601:Eukaryotic Initiation Factor-4A