title: Exceptional antineoplastic activity of a dendritic-cell-targeted vaccine loaded with a Listeria peptide proposed against metastatic melanoma.
abstract: Vaccination with dendritic cells (DCs) is proposed to induce lasting responses against melanoma but its survival benefit in patients needs to be demonstrated. We propose a DC-targeted vaccine loaded with a Listeria peptide with exceptional anti-tumour activity to prevent metastasis of melanoma. Mice vaccinated with vaccines based on DCs loaded with listeriolysin O peptide (91-99) (LLO91-99) showed clear reduction of metastatic B16OVA melanoma size and adhesion, prevention of lung metastasis, enhanced survival, and reversion of immune tolerance. Robust innate and specific immune responses explained the efficiency of DC-LLO91-99 vaccines against B16OVA melanoma. The noTable features of this vaccine related to melanoma reduction were: expansion of immune-dominant LLO91-99-specific CD8 T cells that helped to expand melanoma-specific CD8+ T cells; high numbers of tumour-infiltrating lymphocytes with a cytotoxic phenotype; and a decrease in CD4+CD25high regulatory T cells. This vaccine might be a useful alternative treatment for advanced melanoma, alone or in combination with other therapies.
authors.affiliation: Group of Genomics, Proteomics and Vaccines, Marqués de Valdecilla Research Institute (IDIVAL), Santander, Spain.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Calderon-Gonzalez
authors.affiliation: Group of Genomics, Proteomics and Vaccines, Marqués de Valdecilla Research Institute (IDIVAL), Santander, Spain.|Dermatology Department, Marqués de Valdecilla University Hospital (HUMV), Santander, Spain.
authors.forename: Lucia
authors.initials: L
authors.lastname: Bronchalo-Vicente
authors.affiliation: Pathological Anatomy Department, Marqués de Valdecilla University Hospital (HUMV), Santander, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Freire
authors.affiliation: Group of Genomics, Proteomics and Vaccines, Marqués de Valdecilla Research Institute (IDIVAL), Santander, Spain.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Frande-Cabanes
authors.affiliation: Group of Genomics, Proteomics and Vaccines, Marqués de Valdecilla Research Institute (IDIVAL), Santander, Spain.
authors.forename: Lidia
authors.initials: L
authors.lastname: Alaez-Alvarez
authors.affiliation: Pathological Anatomy Department, Marqués de Valdecilla University Hospital (HUMV), Santander, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Gomez-Roman
authors.affiliation: Dermatology Department, Marqués de Valdecilla University Hospital (HUMV), Santander, Spain.
authors.forename: Sonsóles
authors.initials: S
authors.lastname: Yañez-Diaz
authors.affiliation: Group of Genomics, Proteomics and Vaccines, Marqués de Valdecilla Research Institute (IDIVAL), Santander, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Alvarez-Dominguez
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology / Q000494:pharmacology*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology / Q000494:pharmacology*; D003713:Dendritic Cells / Q000276:immunology*; D006360:Heat-Shock Proteins / Q000276:immunology / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000276:immunology / Q000494:pharmacology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology; D008546:Melanoma, Experimental / Q000276:immunology / Q000473:pathology*; D051379:Mice; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology*
chemical_list: D001427:Bacterial Toxins; D019496:Cancer Vaccines; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes
keywords: Listeria peptides; dendritic cells; immunotherapy; melanoma; vaccines