title: Evaluation of Chair-Side Assays in High Microbiological Caries-Risk Subjects.
abstract: The aim of this study was to evaluate the commercial chair-side assays Saliva-Check Mutans and ClinproTM Cario L-PopTM in high microbiological caries-risk dental students compared with conventional semi-quantitative colony counting culture-based technique as the reference method. Saliva samples from 93 subjects of both sexes aged 18-26 years were seeded (Köhler and Bratthall method) on plates containing SB-20M culture medium method and 12 subjects with high caries risk were selected. These 12 individuals were subjected to determination of caries risk using two commercial rapid detection chair-side assays (Saliva-Check Mutans and ClinproTM Cario L-PopTM) according to the manufacturers' instructions. The results were analyzed by the Kappa correlation test using SAS statistical software. There was a perfect agreement (Kappa=1) among the three caries risk evaluation methods - chair-side assays and semi-quantitative CFU count (control) - in all subjects. The results suggest that the commercial chair-side assays evaluated in this study may be practical and useful to identify high microbiological caries-risk subjects.
authors.affiliation: Department of Preventive Dentistry, Dental School, National University of Tucuman, San Miguel de Tucuman, Argentina.
authors.forename: Marta Estela
authors.initials: ME
authors.lastname: Saravia
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Lea Assed Bezerra
authors.initials: LA
authors.lastname: Silva
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Raquel Assed Bezerra
authors.initials: RA
authors.lastname: Silva
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Marília Pacífico
authors.initials: MP
authors.lastname: Lucisano
authors.affiliation: Department of Restorative Dentistry, Dental School, National University of Córdoba, Córdoba, Argentina.
authors.forename: Andrea Uribe
authors.initials: AU
authors.lastname: Echevarría
authors.affiliation: Department of Restorative Dentistry, Dental School, National University of Córdoba, Córdoba, Argentina.
authors.forename: Jorge Uribe
authors.initials: JU
authors.lastname: Echevarría
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
mesh_terms: D000293:Adolescent; D000328:Adult; D003731:Dental Caries / Q000453:epidemiology*; D005260:Female; D006801:Humans; D008297:Male; D012307:Risk Factors; D013335:Students, Dental; D055815:Young Adult