title: Differential Expression of MicroRNAs in Chronic Obstructive Pulmonary Disease.
abstract: BACKGROUND
  This study aimed to investigate the associations of miRNA with COPD patients.
  
  
  OBJECTIVE
  Chronic obstructive pulmonary disease (COPD) is characterized by progressive and largely irreversible airflow limitation. COPD is one of the most common causes of death globally and it is still a serious public health problem worldwide. Pathogenesis of COPD is multifactorial including genetics and environmental factors.
  
  
  METHODS
  Sixty patients who were diagnosed with COPD according to GOLD guidance and 40 controls were involved in the study. This study was separated into four groups according to GOLD guidance. miR_16, miR_17, miR_29c, miR_92, miR_125, miR_126, miR_146, miR_155, miR_181, mir_122 expressions from the total miRNAs obtained were worked on by using real time-PCR method. The p-values are calculated based on a Student's t-test of the replicate 2^ (- Delta Ct) values for each gene in the control group.
  
  
  RESULTS
  The miRNAs expressions in normal and COPD patients were found differentially. The miR-29c (p = 0.043) and -126 (p = 0.012) were found significantly different compared to control group. When their expressions are evaluated according to stage, miR-92 expression showed down regulation stage II and no change was observed in other miRNAs. miR-29c and miR-126 expressions showed significant differences in stage III and only miR-126 expression showed significant difference in stage IV.
  
  
  CONCLUSIONS
  These results show that miRNA evaluations may give information about the diagnosis, staging and prognosis of the disease. In this study, we demonstrated that miR-29c and -126 are essential for the development of COPD.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Mugla Sitki Kocman University, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kara
authors.affiliation: Department of Chest Diseases, Faculty of Medicine, Firat University, Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Kirkil
authors.affiliation: Department of Chest Diseases, Faculty of Medicine, Mugla Sitki Kocman University, Turkey.
authors.forename: Serdar
authors.initials: S
authors.lastname: Kalemci
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D010641:Phenotype; D011237:Predictive Value of Tests; D011379:Prognosis; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs