title: Effects of the fish spawning inducer ovaprim on vasotocin receptor gene expression in brain and ovary of the catfish Heteropneustes fossilis with a note on differential transcript expression in ovarian follicles.
abstract: Ovaprim (OVP), a commercial formulation of a salmon GnRH analogue and the dopamine receptor-2 blocker domperidone, is a successful spawning inducer for fish breeding. It induces a preovulatory surge in LH, which stimulates the synthesis of a maturation-inducing steroid (MIS, 17,20β-dihydroxy-4-pregnen-3-one) that initiates germinal vesicle breakdown (GVBD) and ovulation. Coincidently, the OVP treatment also stimulates vasotocin (VT) secretion in the brain and ovary of the catfish Heteropneustes fossilis that also stimulates the synthesis of the MIS. VT mediates its effect through V1- and V2-type receptors. In the present study in the catfish, we report that OVP stimulates the expression of VT receptor genes v1a1, v1a2 and v2a in the brain and ovary. A single intraperitoneal administration of OVP (0.5μL/g body weight) or incubation of post-vitellogenic ovarian follicles with 5μL/mL OVP, for 0, 4, 8, 12, 16, and 24h stimulated ovulation and GVBD, respectively, in a time-dependent manner. The OVP treatment in vivo stimulated brain VT receptor transcript levels 4h onwards. The peak expression was noticed at 12h (v1a1), 8 and 12h (v1a2), and 8, 12 and 16h (v2a), coinciding with FOM and ovulation. The VT receptor genes are expressed in the ovarian follicles compartmentally; both v1a1 and v1a2 are expressed in the isolated follicular layer (theca and granulosa) but absent in denuded oocytes. V2a is expressed in the denuded oocytes and not in the follicular layer. The OVP injection stimulated the v1a1 and v1a2 expression from 4h onwards in both intact follicle and isolated follicular layer, the peak expression was observed at 16h. The v2a expression was up-regulated in both intact follicles and denuded oocytes at 4h (denuded oocytes) or 8h (intact follicle) onwards with the peak expression at 12h and 16h (denuded oocytes) or at 16h (intact follicles). Under in vitro conditions, the OVP incubations elicited similar pattern of changes with the peak stimulation at 16h for all the genes. In conclusion, the VT receptor genes are differentially expressed in the ovarian follicles and OVP induced periovulatory stimulation of the VT receptor genes, coinciding with FOM and ovulation.
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: A
authors.initials: A
authors.lastname: Rawat
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: R
authors.initials: R
authors.lastname: Chaube
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi 221005, India. Electronic address: kpjoybhu@gmail.com.
authors.forename: K P
authors.initials: KP
authors.lastname: Joy
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002397:Catfishes / Q000235:genetics / Q000378:metabolism; D004294:Domperidone / Q000494:pharmacology*; D004338:Drug Combinations; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006908:Hydroxyprogesterones / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects / Q000235:genetics; D017483:Receptors, Vasopressin / Q000235:genetics* / Q000378:metabolism; D014668:Vasotocin / Q000378:metabolism; D014818:Vitellogenesis / Q000187:drug effects / Q000235:genetics
chemical_list: D004338:Drug Combinations; D006908:Hydroxyprogesterones; D017483:Receptors, Vasopressin; C527878:ovaprim; C034862:vasotocin receptor; C007433:17,20-dihydroxy-4-pregnen-3-one; D007987:Gonadotropin-Releasing Hormone; D004294:Domperidone; D014668:Vasotocin
keywords: Catfish; Ovaprim; Periovulatory changes; VT receptor gene subtypes