title: Generation and characterization of bovine bone marrow-derived macrophage cell line.
abstract: Macrophages, as the forefront of innate immune defense, have an important role in the host responses to mycobacterial infection. Therefore, a stable macrophage cell line is needed for future bovine immune system research on the bacterial infection. In this study, we established a bovine macrophage cell line by introducing the human telomerase reverse transcriptase (hTERT) gene into bovine bone marrow-derived macrophages (bBMMs). The TERT-bBMMs cells expressed macrophage surface antigen (CD11b, CD282) and upregulated expression of the cytokines IL-1β, IL-6, IL-10, IL-12, TNF-α in response to bacterial invasion. These results demonstrate that this cell line provide reliable cell model system for future studies on interactions between the bovine macrophages and Mycobacterium tuberculosis.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Xiao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Rongxia
authors.initials: R
authors.lastname: Xie
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Wuju
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D015398:Signal Transduction; D019098:Telomerase / Q000096:biosynthesis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; C509186:TERT protein, human; D019098:Telomerase
keywords: bovine; human telomerase reverse transcriptase; immortalization; macrophages; telomerase