title: The effect of fiber size and pore size on cell proliferation and infiltration in PLLA scaffolds on bone tissue engineering.
abstract: The scaffold microstructure has a great impact on cell functions in tissue engineering. Herein, the PLLA scaffolds with hierarchical fiber size and pore size were successfully fabricated by thermal-induced phase separation or combined thermal-induced phase separation and salt leaching methods. The PLLA scaffolds were fabricated as microfibrous scaffolds, microfibrous scaffolds with macropores (50-350 µm), nanofibrous scaffolds with micropores (100 nm to 10 µm), and nanofibrous scaffolds with both macropores and micropores by tailoring selective solvents for forming different fiber size and pre-sieved salts for creating controlled pore size. Among the four kinds of PLLA scaffolds, the nanofibrous scaffolds with both macropores and micropores provided a favorable microenvironment for protein adsorption, cell proliferation, and cell infiltration. The results further confirmed the significance of fiber size and pore size on the biological properties, and a scaffold with both micropores and macropores, and a nanofibrous matrix might have promising applications in bone tissue engineering.
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China wangxjlt@aliyun.com.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China taolou72@aliyun.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Lou
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Wenhua
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Song
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Chunyao
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Cui
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C033616:poly(lactide)
keywords: Pore size; fiber size; hierarchical; scaffold; tissue engineering