title: [Usefulness of conventional polymerase chain reaction for the detection of Mycoplasma hominis, Ureaplasma spp. and Trichomonas vaginalis in female outpatient's genital samples].
abstract: BACKGROUND
  Trichomonas vaginalis, Mycoplasma hominis and Ureaplasma spp. are microorganisms responsible for genitourinary and pregnancy pathologies. Nucleic acid amplification methods have shown several advantages, but have not been widely studied for the detection of these microorganisms.
  
  
  OBJECTIVE
  To implement a conventional polymerase chain reaction (PCR) for the detection of the microorganisms and to compare its results versus the methods currently used at our laboratory.
  
  
  METHODS
  91 available samples were processed by PCR, culture (M. hominis y Ureaplasma spp.) and wet mount (T vaginalis). Results were compared and statistically analyzed by kappa agreement test.
  
  
  RESULTS
  85, 80 and 87 samples resulted in agreement for the detection of M. hominis, Ureaplasma spp. y T. vaginalis, respectively. For M. hominis and Ureaplasma spp., agreement was substantial, whereas for T. vaginalis it was moderate, however, for the latter, PCR detected more cases than wet mount.
  
  
  CONCLUSIONS
  We recommend the implementation of PCR for detection of T. vaginalis whereas culture kit is still a useful method for the other microorganisms.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Alarcón
authors.forename: Gabriela
authors.initials: G
authors.lastname: Barraza
authors.forename: Andrea
authors.initials: A
authors.lastname: Vera
authors.forename: Aniela
authors.initials: A
authors.lastname: Wozniak
authors.forename: Patricia
authors.initials: P
authors.lastname: García
mesh_terms: D005260:Female; D006801:Humans; D009175:Mycoplasma Infections / Q000175:diagnosis*; D019535:Mycoplasma hominis / Q000235:genetics* / Q000302:isolation & purification; D010045:Outpatients; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D014245:Trichomonas Infections / Q000175:diagnosis*; D014246:Trichomonas vaginalis / Q000235:genetics*; D014509:Ureaplasma / Q000235:genetics* / Q000302:isolation & purification; D016869:Ureaplasma Infections / Q000175:diagnosis*