title: MARCKS-like protein is an initiating molecule in axolotl appendage regeneration.
abstract: Identifying key molecules that launch regeneration has been a long-sought goal. Multiple regenerative animals show an initial wound-associated proliferative response that transits into sustained proliferation if a considerable portion of the body part has been removed. In the axolotl, appendage amputation initiates a round of wound-associated cell cycle induction followed by continued proliferation that is dependent on nerve-derived signals. A wound-associated molecule that triggers the initial proliferative response to launch regeneration has remained obscure. Here, using an expression cloning strategy followed by in vivo gain- and loss-of-function assays, we identified axolotl MARCKS-like protein (MLP) as an extracellularly released factor that induces the initial cell cycle response during axolotl appendage regeneration. The identification of a regeneration-initiating molecule opens the possibility of understanding how to elicit regeneration in other animals.
authors.affiliation: DFG Research Center for Regenerative Therapies (CRTD), Technische Universität Dresden, Fetscherstrasse 105, 01307 Dresden, Germany.|Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Takuji
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Karolinska Institute, Department of Cell and Molecular Biology, Centre of Developmental Biology for Regenerative Medicine, SE-171 77 Stockholm, Sweden.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Rico
authors.initials: R
authors.lastname: Barsacchi
authors.affiliation: Karolinska Institute, Department of Cell and Molecular Biology, Centre of Developmental Biology for Regenerative Medicine, SE-171 77 Stockholm, Sweden.
authors.forename: Andras
authors.initials: A
authors.lastname: Simon
authors.affiliation: DFG Research Center for Regenerative Therapies (CRTD), Technische Universität Dresden, Fetscherstrasse 105, 01307 Dresden, Germany.|Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Elly M
authors.initials: EM
authors.lastname: Tanaka
mesh_terms: D000558:Ambystoma mexicanum / Q000293:injuries / Q000502:physiology*; D000673:Amputation, Traumatic / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D003001:Cloning, Molecular; D005121:Extremities / Q000293:injuries / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000502:physiology; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D009673:Notophthalmus viridescens / Q000235:genetics / Q000293:injuries / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D013623:Tail / Q000166:cytology / Q000293:injuries / Q000502:physiology; D014945:Wound Healing / Q000502:physiology; D014981:Xenopus; D015027:Zebrafish
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C000619710:MARCKS protein, human; C000619711:Marcks protein, mouse; D008565:Membrane Proteins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate