title: Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production.
abstract: OBJECTIVE
  To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection.
  
  
  RESULTS
  Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however, H3N2 virus grown in MDCK-SIAT1 had lower viral titers in comparison with MDCK cells. The optimized MOIs to infect the cells on plates and microcarrier were selected 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively.
  
  
  CONCLUSIONS
  MDCK-SIAT1 cells may be considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic stability and high titer of influenza virus production.
authors.affiliation: Departments of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.|Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Abdoli
authors.affiliation: Departments of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hoorieh
authors.initials: H
authors.lastname: Soleimanjahi
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Jamali
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Mehrbod
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Shima
authors.initials: S
authors.lastname: Gholami
authors.affiliation: Immunoregulation Research Center, Shahed University, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Kianmehr
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Feizi
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Saleh
authors.affiliation: Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Bahrami
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Talat
authors.initials: T
authors.lastname: Mokhtari-Azad
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Abdoli
authors.affiliation: Influenza Research Lab, Department of Virology, Pasteur Institute of Iran, Tehran, Iran. mtkheiri@gmail.com.
authors.forename: Masoumeh Tavassoti
authors.initials: MT
authors.lastname: Kheiri
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002645:Chickens; D003911:Dextrans; D004285:Dogs; D006386:Hemagglutination Tests / Q000379:methods; D006387:Hemagglutination, Viral; D053118:Influenza A Virus, H1N1 Subtype / Q000254:growth & development*; D053122:Influenza A Virus, H3N2 Subtype / Q000254:growth & development*; D061985:Madin Darby Canine Kidney Cells / Q000166:cytology* / Q000821:virology*; D014765:Viral Vaccines
chemical_list: D003911:Dextrans; D014765:Viral Vaccines; C052839:Cytodex
keywords: Influenza virus; MDCK cells; MDCK-SIAT1 cells; Microcarrier; Vaccine