title: Expression of Leptin and Sirtuin-1 is associated with poor prognosis in patients with osteosarcoma.
abstract: Sirtuin-1 (SIRT1) is a downstream target of Leptin, and its inhibition promotes p53-mediated apoptosis. This study aimed to evaluate the expression and prognostic significance of Leptin and SIRT1 in osteosarcoma. Leptin and SIRT1 levels in osteosarcoma samples from 89 patients were evaluated by immunohistochemical staining. The correlations between Leptin and SIRT1 expression with clinical parameters were analyzed by Spearman's test and Pearson's chi-squared test. Prognostic factors were identified by Univariate and multivariate Cox regression analysis. We found that Leptin and SIRT1 expression was low in 23.6% and 20.2%; moderate in 25.8% and 24.7%; and high in 50.5% and 55.1% of patients with osteosarcoma, respectively. Both Leptin and SIRT1 expression were significantly associated with the Enneking stage, distant metastasis and neo-adjuvant chemotherapy. Leptin expression and SIRT1 expression were significantly correlated and they were significantly associated with shorter overall survival. Among osteosarcoma patients who received neo-adjuvant chemotherapy, both Leptin and SIRT1 expression were significantly associated with overall survival of osteosarcoma patients in univariate analysis, but only SIRT1 expression was significantly associated with overall survival of osteosarcoma patients in multivariate analysis. In conclusion, Leptin and SIRT1 expressions are significantly associated with shorter overall survival of osteosarcoma patients, and SIRT1 expression is a significant independent prognostic indicator in patients with osteosarcoma.
authors.affiliation: Key Laboratory of Physiology and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, 20 Nanerhuan East Road, Shijiazhuang 050024, Hebei, China; Department of Orthopedics, The Fourth Hospital of Hebei Medical University, 12 Health Road, Shijiazhuang 050011, Hebei, China.
authors.forename: Helin
authors.initials: H
authors.lastname: Feng
authors.affiliation: Department of Orthopedics, The Fourth Hospital of Hebei Medical University, 12 Health Road, Shijiazhuang 050011, Hebei, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, The Fourth Hospital of Hebei Medical University, 12 Health Road, Shijiazhuang 050011, Hebei, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The Fourth Hospital of Hebei Medical University, 12 Health Road, Shijiazhuang 050011, Hebei, China.
authors.forename: Jianfa
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Physiology and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, 20 Nanerhuan East Road, Shijiazhuang 050024, Hebei, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Xie
authors.affiliation: Key Laboratory of Physiology and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, 20 Nanerhuan East Road, Shijiazhuang 050024, Hebei, China. Electronic address: fulugao0311@126.com.
authors.forename: Fulu
authors.initials: F
authors.lastname: Gao
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D001859:Bone Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D002648:Child; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D020738:Leptin / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D012516:Osteosarcoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D016016:Proportional Hazards Models; D056564:Sirtuin 1 / Q000032:analysis / Q000096:biosynthesis*; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D020738:Leptin; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Leptin; Osteosarcoma; Prognostic indicator; Sirtuin-1