title: Gene expression profile changes in Eisenia fetida chronically exposed to PFOA.
abstract: Eisenia fetida is a terrestrial organism, which can be used to diagnose sub-lethal concentrations of PFOA by using molecular biomarkers. In order to identify potential molecular biomarkers, we have exposed E. fetida to 10 mg/kg of PFOA in soil for 8 months. The mRNA isolation, sequencing, transcriptome assembly followed by differential gene expression studies have revealed that genes that are involved in apoptotic process, reproduction, calcium signalling, neuronal development and lipid metabolism are predominantly affected. Highly specific genes that are altered by PFOA can be further validated and used as biomarker to detect sub-lethal concentrations of PFOA in the soil.
authors.affiliation: Centre for Environmental Risk Assessment and Remediation, University of South Australia, Mawson Lakes, Adelaide, SA, 5095, Australia.|Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE), Mawson Lakes, Adelaide, SA, 5095, Australia.
authors.forename: Srinithi
authors.initials: S
authors.lastname: Mayilswami
authors.affiliation: Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE), Mawson Lakes, Adelaide, SA, 5095, Australia. Kannan.krishnan@newcastle.edu.au.|Global Centre for Environmental Remediation, Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia. Kannan.krishnan@newcastle.edu.au.
authors.forename: Kannan
authors.initials: K
authors.lastname: Krishnan
authors.affiliation: Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE), Mawson Lakes, Adelaide, SA, 5095, Australia.|Global Centre for Environmental Remediation, Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia.
authors.forename: Mallavarapu
authors.initials: M
authors.lastname: Megharaj
authors.affiliation: Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE), Mawson Lakes, Adelaide, SA, 5095, Australia.|Global Centre for Environmental Remediation, Faculty of Science and Information Technology, The University of Newcastle, Callaghan, NSW, 2308, Australia.
authors.forename: Ravi
authors.initials: R
authors.lastname: Naidu
mesh_terms: D000818:Animals; D015415:Biomarkers; D002210:Caprylates / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D015870:Gene Expression; D046228:Microarray Analysis; D009835:Oligochaeta / Q000235:genetics*; D012989:Soil Pollutants / Q000633:toxicity*; D059467:Transcriptome
chemical_list: D015415:Biomarkers; D002210:Caprylates; D005466:Fluorocarbons; D012989:Soil Pollutants; C023036:perfluorooctanoic acid
keywords: Differential gene expression; Eisenia fetida; Perfluorooctanoic acid (PFOA); Toxicogenomics; Transcriptome assembly