title: Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility.
abstract: The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.
authors.affiliation: School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China. zhangkun@zzu.edu.cn.|Center of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Zhengzhou University, 40 University Road, Zhengzhou, 450052, People's Republic of China. zhangkun@zzu.edu.cn.|Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China. zhangkun@zzu.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
authors.forename: Jun-ying
authors.initials: JY
authors.lastname: Chen
authors.affiliation: Jiangsu Heze Allian Cells Bioscience Co., Ltd, Changzhou, 213000, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
authors.forename: Jing-an
authors.initials: JA
authors.lastname: Li
authors.affiliation: School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou, 450001, People's Republic of China.|Center of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Zhengzhou University, 40 University Road, Zhengzhou, 450052, People's Republic of China.
authors.forename: Fang-xia
authors.initials: FX
authors.lastname: Guan
authors.affiliation: Key Laboratory for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D024141:Collagen Type IV / Q000737:chemistry / Q000502:physiology*; D004285:Dogs; D042783:Endothelial Cells / Q000502:physiology; D005263:Femoral Artery; D006493:Heparin / Q000737:chemistry* / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D024141:Collagen Type IV; D006493:Heparin; D014025:Titanium