title: Assessment in vitro of the genotoxicity, antigenotoxicity and antioxidant of Ceratonia siliqua L. extracts in murine leukaemia cells L1210 by comet assay.
abstract: Genotoxicity of Ceratonia siliqua extracts, was investigated by assessing their capacity to induce nucleus DNA degradation of murine leukaemia cells L1210, using the "Comet assay". The ability of total oligomer flavonoids (TOF) and aqueous extracts to protect cell DNA against oxidative stress induced by H2O2, was performed by pre- co or post-treatment of cells with the before mentioned extracts for different periods preceding exposure to H2O2 stress. No significant genotoxic effect was detected at different exposure times, except at the lowest concentration of TOF extract (16.25 μg/ml). It appears that extracts decreased DNA damage, induced by H2O2. Both of TOF and aqueous extracts exhibited cellular antioxidant capacity, with EC50 values of respectively <16.25 and < 35 μg/ml, as well as, a protective capacity against lipidperoxidation inducing using L1210 cells line as a cellular model. MDA inhibition percentages reached 88.43% and 90.52% with respectively 35.5 μg/ml of TOF extract and 70 μg/ml of aqueous extract. Antioxidant properties of carob leaf extracts revealed by our study make a good antioxidant protection and thus a good candidate as food addition component.
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire. Faculté de médecine dentaire. Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie; Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 ». Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
authors.forename: Aïcha
authors.initials: A
authors.lastname: Sassi
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire. Faculté de médecine dentaire. Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie; Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 ». Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire. Faculté de médecine dentaire. Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie; Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 ». Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mustapha
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire. Faculté de médecine dentaire. Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie; Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 ». Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
authors.forename: Imen
authors.initials: I
authors.lastname: Mokdad-Bzeouich
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire. Faculté de médecine dentaire. Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie; Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 ». Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
authors.forename: Fadwa
authors.initials: F
authors.lastname: Chaabane
authors.affiliation: Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 ». Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.affiliation: Laboratoire de biologie cellulaire et moléculaire. Faculté de médecine dentaire. Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie; Unité de Substances Naturelles Bioactives et Biotechnologie « UR12ES12 ». Faculté de pharmacie de Monastir, Université de Monastir, Rue Avicenne 5000 Monastir, Tunisie. Electronic address: leila.chekir@laposte.net.
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D015415:Biomarkers / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry / Q000633:toxicity; D006861:Hydrogen Peroxide / Q000633:toxicity; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009153:Mutagens / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010946:Plants, Medicinal; D018570:Risk Assessment
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D015415:Biomarkers; D009153:Mutagens; D010936:Plant Extracts; D008315:Malondialdehyde; D006861:Hydrogen Peroxide
keywords: Antigenotoxicity; Cellular antioxidant activity; Ceratonia siliqua; Comet assay; Lipid peroxidation; Polyphenols