title: Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae.
abstract: Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.
authors.affiliation: Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Japan.|Graduate School of Agricultural and Life Sciences, the University of Tokyo, Japan.|Atmosphere and Ocean Research Institute, the University of Tokyo, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Endo
authors.affiliation: Atmosphere and Ocean Research Institute, the University of Tokyo, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Japan.
authors.forename: Toshiki
authors.initials: T
authors.lastname: Uji
authors.affiliation: Institute for Food Sciences, Hirosaki University, Japan.
authors.forename: Naotsune
authors.initials: N
authors.lastname: Saga
authors.affiliation: Atmosphere and Ocean Research Institute, the University of Tokyo, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Inoue
authors.affiliation: College of Life Sciences, Zhejiang University, China.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D015870:Gene Expression; D005827:Genetics, Microbial / Q000379:methods*; D042822:Genomic Instability; D058087:Haptophyta / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D011994:Recombinant Proteins