title: Immunological evaluation of the entirely carbohydrate-based Thomsen-Friedenreich - PS B conjugate.
abstract: PS B, a naturally occurring CD4(+) T-cell simulating zwitterionic polysaccharide from Bacteroides fragilis ATCC 25285/NCTC 9343, was conjugated with aminooxy Thomsen Friedenreich (TF or T) [α-d-Gal-(1,3)-β-d-GalNAc-ONH2] tumor antigen. Immunization in Jax C57BL/6, followed by ELISA revealed IgM and IgG antibody TF specificity. FACS data noted preferential binding to TF-laced MCF-7 cells but not to HCT-116 cells.
authors.affiliation: Department of Chemistry and Biochemistry, University of Toledo, Wolfe Hall, Room 2232B, 2801 W. Bancroft Street, Toledo, OH 43606-3390, USA. Peter.Andreana@utoledo.edu.
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Trabbic
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bourgault
authors.forename: Mengchao
authors.initials: M
authors.lastname: Shi
authors.forename: Matthew
authors.initials: M
authors.lastname: Clark
authors.forename: Peter R
authors.initials: PR
authors.lastname: Andreana
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry / Q000276:immunology*; D001441:Bacteroides fragilis / Q000737:chemistry; D002236:Carbohydrate Conformation; D004797:Enzyme-Linked Immunosorbent Assay; D045325:HCT116 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D007075:Immunoglobulin M / Q000737:chemistry / Q000276:immunology; D061986:MCF-7 Cells; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011134:Polysaccharides; C016467:Thomsen-Friedenreich antigen