title: Determination of antazoline and tetrahydrozoline in ophthalmic solutions by capillary electrophoresis and stability-indicating HPLC methods.
abstract: Capillary electrophoretic (CE) and high performance liquid chromatographic (HPLC) methods were developed and optimized for the determination of antazoline (ANT) and tetrahydrozoline (TET) in ophthalmic formulations. Optimum electrophoretic conditions were achieved using a background electrolyte of 20mM phosphate buffer at pH 7.0, a capillary temperature of 25°C, a separation voltage of 22 kV and a pressure injection of the sample at 50 mbar for 17s. HPLC analysis was performed with Kinetex (150 × 4.6mm ID × 5 μm) (Phenomenex, USA) analytical column with 1 mL min(-1) flow rate of mobile phase which consisted of 0.05% TFA in bidistilled water (pH adjusted to 3.0 with 5M NaOH) and acetonitrile/buffer in the ratio of 63:37 (v/v) at room temperature. Injection volume of the samples was 10 μL and the wavelength of the detector was set at 215 nm for monitoring both analytes. Calibration graphs showed a good linearity with a coefficient of determination (R(2)) of at least 0.998 for both methods. Intraday and interday precision (expressed as RSD%) were lower than 2.8% for CE and 0.92% for HPLC. The developed methods were demonstrated to be simple and rapid for the determination of ANT and TET in ophthalmic solutions providing recoveries in the range between 97.9 and 102.70% for CE and HPLC.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gumustas
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey; Department of Analytical Chemistry, Faculty of Pharmacy, Near East University, 99138 Mersin 10, Turkey.
authors.forename: Usama
authors.initials: U
authors.lastname: Alshana
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Nusret
authors.initials: N
authors.lastname: Ertas
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
authors.forename: Nilgun Gunden
authors.initials: NG
authors.lastname: Goger
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
authors.forename: Sibel A
authors.initials: SA
authors.lastname: Ozkan
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey. Electronic address: buslu@pharmacy.ankara.edu.tr.
authors.forename: Bengi
authors.initials: B
authors.lastname: Uslu
mesh_terms: D000865:Antazoline / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007093:Imidazoles / Q000032:analysis*; D009883:Ophthalmic Solutions / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D009883:Ophthalmic Solutions; D000865:Antazoline; C005810:tetrahydrozoline
keywords: Antazoline; Capillary electrophoresis; Core shell; HPLC; Ophthalmic solutions; Tetrahydrozoline