title: In Vitro Antioxidant Assays.
abstract: Various chemical in vitro assays have been developed to measure antioxidant capacities of plant products. Despite the recent popularity in the antioxidant research, the lack of standardized assays to compare research results from different research groups has been a major challenge. The examination of various antioxidant assays is required for the development of standard methods that are broadly applicable by researchers and industry. However, due to the complex nature of biological systems, there is no single universal method for measuring antioxidant capacity. Antioxidant methods such as DPPH*, ABTS+, nitric oxide, super oxide, metal chelating confirming the free radical scavenging property of the plants with widely used methods are simplified in this chapter.
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D065132:Bioprospecting / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects; D055808:Drug Discovery / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D009569:Nitric Oxide / Q000737:chemistry; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D064209:Phytochemicals / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D007502:Iron Chelating Agents; D064209:Phytochemicals; D010936:Plant Extracts; D009569:Nitric Oxide