title: Combination of Curcumin with an Anti-Transferrin Receptor Antibody Suppressed the Growth of Malignant Gliomas In vitro.
abstract: OBJECTIVE
  Transferrin receptor (TfR) has been used as a target for the molecular cancer therapy due to its higher expression in a variety of tumors. Anti-TfR antibodies combined with chemotherapeutic drugs has showed great potential as a possible cancer therapeutic strategy. In our study, we investigated the anti-tumor effects of anti-TfR monoclonal antibody (mAb) alone or in combination with curcumin in vitro.
  
  
  METHODS
  We detected the apoptosis, proliferation and cell cycle of glioma cells after treated with anti-TfR mAb and curcumin alone or the combinations by flow cytometer.
  
  
  RESULTS
  Anti-TfR mAb or curcumin could inhibit proliferation of tumor cells. Anti-TfR mAb marked S phase arrest and curcumin induced G2/M arrest in tumor cells. When anti-TfR mAb and curcumin were used simultaneously, a synergistic effect was detected in relation to tumor growth inhibition and the induction of cells necrosis.
  
  
  CONCLUSIONS
  These results provided a potential role of anti-TfR mAb-containing curcumin in the treatment for gliomas.
authors.affiliation: Hubei Polytechnic Institute, School of Nursing, Department of Basic Medical, Xiaogan, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wen
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Cheng
authors.forename: Dahai
authors.initials: D
authors.lastname: Hu
authors.forename: Wenmin
authors.initials: W
authors.lastname: Li
authors.forename: Jianli
authors.initials: J
authors.lastname: Ha
authors.forename: Zhaochang
authors.initials: Z
authors.lastname: Kang
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhang
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Huang
authors.forename: Si
authors.initials: S
authors.lastname: Wu
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology*; D005434:Flow Cytometry; D005910:Glioma / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D011990:Receptors, Transferrin / Q000037:antagonists & inhibitors*
chemical_list: D000911:Antibodies, Monoclonal; D011990:Receptors, Transferrin; D003474:Curcumin