title: Determination of a potential antitumor quassinoid in rat plasma by UPLC-MS/MS and its application in a pharmacokinetic study.
abstract: A sensitive UPLC-MS/MS method was developed and validated for the determination of brusatol in rat plasma. Chromatographic separation was carried out on a C18 column using methanol and 10mM ammonium acetate containing 0.1% (v/v) formic acid (55:45, v/v). The lower limit of quantification (LLOQ) was 1.0 ng/mL for brusatol in plasma. The intra- and inter-day precision for the analyte ranged from 3.2% to 9.2% and 1.3% to 7.8%, and the accuracy was between 97.3% and 108.5%. The method was successfully applied in a pharmacokinetic study of brusatol following intravenous injection (0.5, 1.0, and 2.0mg/kg) of brusatol.
authors.affiliation: Breast Surgery, Liaoning Cancer Hospital & Institute, Shenyang 110042, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Infection, Liaoning Cancer Hospital & Institute, Shenyang 110042, China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Endocrine Surgery, the People's Hospital of Liaoning Province, Shenyang 110016, China.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Cui
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Xiao
authors.affiliation: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062, China. Electronic address: depon2005@126.com.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Breast Surgery, Liaoning Cancer Hospital & Institute, Shenyang 110042, China. Electronic address: daqingjiang11@sina.com.
authors.forename: Daqing
authors.initials: D
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D036702:Quassins / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D036702:Quassins
keywords: Brusatol; Pharmacokinetic study; Quassinoid; Rat plasma; UPLC–MS/MS