title: [Transcriptome analysis of bioenergy plant Miscanthus sinensis Anderss by RNA-Seq].
abstract: Miscanthus sinensis Anderss is a perennial C4-grass. It is a promising bioenergy plant, which has been proposed as general feedstock for biomass and lignocellulosic biofuel production. In this study, the flower and leaf buds transcriptomes of Miscanthus sinensis Anderss were sequenced by the platform of Illumina HiSeq 2000. In total 98 326 Unigenes were generated by de novo assembly with an average length of 822 bp and N50 of 1 023 bp. Based on the NR, NT, Swiss-Prot, KEGG, GO and COG databases (Evalue < le-5), 74 134 (75.40%) Unigenes were annotated. A total of 45 507 Unigenes were mapped into different GO terms. In KEGG pathways identification, 36 710 sequences were assigned to 128 KEGG pathways. Sorghum bicolor (37 731, 60.86%), Zea mays (16 258, 26.22%), and Oryza sativa (3 065, 4.94%) showed high similarity to Miscanthus sinensis Anderss. And 24 photosynthesis-related enzyme genes were identified. The result provides a foundation for further characterizing the functional genes in Miscanthus sinensis Anderss.
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wang
authors.forename: Man
authors.initials: M
authors.lastname: Yu
authors.forename: Kai
authors.initials: K
authors.lastname: Cao
authors.forename: Li
authors.initials: L
authors.lastname: Zhuang
authors.forename: Changxu
authors.initials: C
authors.lastname: Xu
authors.forename: Weidong
authors.initials: W
authors.lastname: Cao
mesh_terms: D056804:Biofuels; D020869:Gene Expression Profiling; D017343:Genes, Plant; D006109:Poaceae / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D056804:Biofuels; D018749:RNA, Plant