title: The Flavodiiron Protein Flv3 Functions as a Homo-Oligomer During Stress Acclimation and is Distinct from the Flv1/Flv3 Hetero-Oligomer Specific to the O2 Photoreduction Pathway.
abstract: The flavodiiron proteins (FDPs) Flv1 and Flv3 in cyanobacteria function in photoreduction of O2to H2O, without concomitant formation of reactive oxygen species, known as the Mehler-like reaction. Both Flv1 and Flv3 are essential for growth under fluctuating light (FL) intensities, providing protection for PSI. Here we compared the global transcript profiles of the wild type (WT), Δflv1 and Δflv1/Δflv3 grown under constant light (GL) and FL. In the WT, FL induced the largest down-regulation in transcripts involved in carbon-concentrating mechanisms (CCMs), while those of the nitrogen assimilation pathways increased as compared with GL. Already under GL the Δflv1/Δflv3 double mutant demonstrated a partial down-regulation of transcripts for CCM and nitrogen metabolism, while in FL conditions the transcripts for nitrogen assimilation were strongly down-regulated. Many alterations were specific only for Δflv1/Δflv3, and not detected in Δflv1, suggesting that certain transcripts are affected primarily because of the lack of flv3 By constructing the strains overproducing solely either Flv1 or Flv3, we demonstrate that the homo-oligomers of these proteins also function in acclimation of cells to FL, by catalyzing reactions with as yet unidentified components, while the presence of both Flv1 and Flv3 is a prerequisite for the Mehler-like reaction and thus the electron transfer to O2Considering the low expression of flv1, it is unlikely that the Flv1 homo-oligomer is present in the WT.
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Henna
authors.initials: H
authors.lastname: Mustila
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Pasi
authors.initials: P
authors.lastname: Paananen
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Battchikova
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Anita
authors.initials: A
authors.lastname: Santana-Sánchez
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Muth-Pawlak
authors.affiliation: Institut Biowissenschaften, Pflanzenphysiologie, Universität Rostock, Albert-Einstein-Str. 3, D-18059 Rostock, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hagemann
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland allahve@utu.fi evaaro@utu.fi.
authors.forename: Eva-Mari
authors.initials: EM
authors.lastname: Aro
authors.affiliation: Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland allahve@utu.fi evaaro@utu.fi.
authors.forename: Yagut
authors.initials: Y
authors.lastname: Allahverdiyeva
mesh_terms: D000064:Acclimatization / Q000528:radiation effects*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics / Q000528:radiation effects; D004579:Electron Transport / Q000528:radiation effects; D005420:Flavoproteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008027:Light; D013058:Mass Spectrometry; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010641:Phenotype; D055668:Photochemical Processes / Q000528:radiation effects*; D010788:Photosynthesis / Q000528:radiation effects; D055503:Protein Multimerization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000528:radiation effects*; D046939:Synechocystis / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000528:radiation effects; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics / Q000528:radiation effects
chemical_list: D001426:Bacterial Proteins; D005420:Flavoproteins; D012333:RNA, Messenger; D002245:Carbon Dioxide; D010100:Oxygen
keywords: Mehler-like reaction; Synechocystis sp. PCC 6803; fluctuating light; gene expression; photosynthesis