title: Dynamic of expression and localization of cannabinoid-degrading enzymes FAAH and MGLL in relation to CB1 during meiotic maturation of human oocytes.
abstract: The endogenous cannabinoid system has been characterized in some female reproductive organs but little is known about the expression and localization pattern of cannabinoid-degrading enzymes in relation to the CB1 cannabinoid receptor in human oocytes. In this study, we focus on the investigation of the presence and differential distribution of fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGLL) in relation to CB1 during the maturation of human oocytes. We used a total of 290 human oocytes not suitable for in vitro fertilization/intracytoplasmic sperm injection (ICSI): germinal-vesicle (GV) and metaphase-I (MI) stages and metaphase-II (MII) oocytes that had not developed into an embryo after ICSI. Cannabinoid-degrading enzymes and the cannabinoid CB1 receptor were present in human oocytes. Specifically, FAAH was detected in the periphery of the oocyte from the GV to MI stage and co-localized with CB1. Later, by the MII stage, FAAH was spread within the oocyte, whereas MGLL immunostaining was homogeneous across the oocyte at all stages of maturation and only overlapped with CB1 at the GV stage. This coordinated redistribution of cannabinoid system proteins suggests a role for this system in the maturation of the female gamete.
authors.affiliation: Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain. e.agirregoitia@ehu.eus.
authors.forename: Ekaitz
authors.initials: E
authors.lastname: Agirregoitia
authors.affiliation: Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Lide
authors.initials: L
authors.lastname: Totorikaguena
authors.affiliation: Human Reproduction Unit, Cruces University Hospital, University of the Basque Country, Plaza de Cruces s/n, Barakaldo, 48903, Bizkaia, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: Expósito
authors.affiliation: Human Reproduction Unit, Cruces University Hospital, University of the Basque Country, Plaza de Cruces s/n, Barakaldo, 48903, Bizkaia, Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Mendoza
authors.affiliation: Human Reproduction Unit, Cruces University Hospital, University of the Basque Country, Plaza de Cruces s/n, Barakaldo, 48903, Bizkaia, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Matorras
authors.affiliation: Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Bizkaia, Spain.
authors.forename: Naiara
authors.initials: N
authors.lastname: Agirregoitia
mesh_terms: D000328:Adult; D000581:Amidohydrolases / Q000378:metabolism*; D002186:Cannabinoids / Q000378:metabolism*; D002454:Cell Differentiation; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008540:Meiosis; D008994:Monoacylglycerol Lipases / Q000378:metabolism*; D009865:Oocytes / Q000166:cytology* / Q000201:enzymology*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism*; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D002186:Cannabinoids; D008994:Monoacylglycerol Lipases; D012333:RNA, Messenger; D043884:Receptor, Cannabinoid, CB1; D000099252:Fatty Acid Amide Hydrolases
keywords: Enzyme; Gamete; Maturation; Meiosis; Resumption