title: Combinatorial Generation of Chemical Diversity by Redox Enzymes in Chaetoviridin Biosynthesis.
abstract: Chaetoviridins constitute a large family of structurally related secondary metabolites isolated from Chaetomium fungi. To elucidate the biosynthesis pathway and understand how the chemical diversity of chaetoviridins is generated, gene deletion and in vitro characterization of the four post-PKS modifications enzymes were undertaken. CazL and CazP were identified to have substrate promiscuity that facilitates the formation of nonchlorinated analogues. In addition, enzymatic oxidation and reduction combined with spontaneous dehydration and lactonization of the intermediates further expand the chemical diversity.
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California , Los Angeles, California 90095, United States.
authors.forename: Jaclyn M
authors.initials: JM
authors.lastname: Winter
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kishimoto
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California , Los Angeles, California 90095, United States.|Department of Chemical and Biomolecular Engineering, and Department of Chemistry and Biochemistry, University of California , Los Angeles, California 90095, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D053898:Biosynthetic Pathways; D002597:Chaetomium / Q000737:chemistry*; D005663:Furans / Q000737:chemistry / Q000378:metabolism*; D017353:Gene Deletion; D015394:Molecular Structure; D010084:Oxidation-Reduction; D048630:Polyketide Synthases / Q000378:metabolism*; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005663:Furans; D011753:Pyrones; C579267:chaetoviridin A; D048630:Polyketide Synthases