title: Protective effect of hyaluronic acid on cryopreserved boar sperm.
abstract: This study aimed to evaluate the effects of supplementing freezing and thawing media with hyaluronic acid (HA) on the quality parameters of frozen-thawed boar spermatozoa. Boar semen samples were collected from seven mature Yorkshire boars once a week using the gloved hand technique; these samples were frozen-thawed in the extender with added HA. Boar sperm was cryopreserved in the extender with HA added at concentrations of 0 (used as control), 4, 6, 8, 8 and 12mg/L, and their effects on the quality of frozen-thawed boar sperm were evaluated. HA addition to the extender significantly improved sperm motility, sperm membrane integrity, mitochondrial activity, acrosomal integrity, superoxide dismutase and catalase activity, but decreased sperm malondialdehyde level (p<0.05). Therefore, HA could be a promising cryoprotectant for boar sperm.
authors.affiliation: Department of Clinical Laboratory, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qian
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, 1 Yingkou Village, Lanzhou 730070, China. Electronic address: yusjgs@sina.com.
authors.forename: Sijiu
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Clinical Laboratory, The Second People's Hospital of Lianyungang, 41 East Hailian Road, Xinpu 222006, China. Electronic address: zhouylyg@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015925:Cryopreservation; D019610:Cytoprotection / Q000187:drug effects*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008297:Male; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D013552:Swine
chemical_list: D000975:Antioxidants; D006820:Hyaluronic Acid
keywords: Cryopreservation; Hyaluronic acid; Sperm