title: The specific role of plastidial glycolysis in photosynthetic and heterotrophic cells under scrutiny through the study of glyceraldehyde-3-phosphate dehydrogenase.
abstract: The cellular compartmentalization of metabolic processes is an important feature in plants where the same pathways could be simultaneously active in different compartments. Plant glycolysis occurs in the cytosol and plastids of green and non-green cells in which the requirements of energy and precursors may be completely different. Because of this, the relevance of plastidial glycolysis could be very different depending on the cell type. In the associated study, we investigated the function of plastidial glycolysis in photosynthetic and heterotrophic cells by specifically driving the expression of plastidial glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in a glyceraldehyde-3-phosphate dehydrogenase double mutant background (gapcp1gapcp2). We showed that GAPCp is not functionally significant in photosynthetic cells, while it plays a crucial function in heterotrophic cells. We also showed that (i) GAPCp activity expression in root tips is necessary for primary root growth, (ii) its expression in heterotrophic cells of aerial parts and roots is necessary for plant growth and development, and (iii) GAPCp is an important metabolic connector of carbon and nitrogen metabolism through the phosphorylated pathway of serine biosynthesis (PPSB). We discuss here the role that this pathway could play in the control of plant growth and development.
authors.affiliation: a Departament de Biologia Vegetal, Facultat de Farmàcia , Universitat de València , Spain.|b Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València , Dr Moliner 50, 46100 Burjassot , Spain.
authors.forename: Armand Djoro
authors.initials: AD
authors.lastname: Anoman
authors.affiliation: a Departament de Biologia Vegetal, Facultat de Farmàcia , Universitat de València , Spain.|b Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València , Dr Moliner 50, 46100 Burjassot , Spain.
authors.forename: María
authors.initials: M
authors.lastname: Flores-Tornero
authors.affiliation: a Departament de Biologia Vegetal, Facultat de Farmàcia , Universitat de València , Spain.|b Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València , Dr Moliner 50, 46100 Burjassot , Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Rosa-Telléz
authors.affiliation: a Departament de Biologia Vegetal, Facultat de Farmàcia , Universitat de València , Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Muñoz-Bertomeu
authors.affiliation: a Departament de Biologia Vegetal, Facultat de Farmàcia , Universitat de València , Spain.|b Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València , Dr Moliner 50, 46100 Burjassot , Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Segura
authors.affiliation: a Departament de Biologia Vegetal, Facultat de Farmàcia , Universitat de València , Spain.|b Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), Universitat de València , Dr Moliner 50, 46100 Burjassot , Spain.
authors.forename: Roc
authors.initials: R
authors.lastname: Ros
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002244:Carbon / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006019:Glycolysis; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010766:Phosphorylation; D010788:Photosynthesis; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D018087:Plastids / Q000378:metabolism*; D012694:Serine / Q000096:biosynthesis
chemical_list: D012694:Serine; D002244:Carbon; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009584:Nitrogen
keywords: 2-oxoglutarate; glyceraldehyde-3-phosphate dehydrogenase; phosphorylated pathway of serine biosynthesis; plastidial glycolysis; serine