title: Impedance Analysis of Colloidal Gold Nanoparticles in Chromatography Paper for Quantitation of an Immunochromatographic Assay.
abstract: A detection method of gold nanoparticles in chromatography paper has been developed for a simple, cost-effective and reliable quantitation of immunochromatographic strip test. The time courses of the solution resistance in chromatography paper with the gold nanoparticles solution are electrochemically measured by chrono-impedimetry. The dependence of the solution resistance on the concentration of gold nanoparticles has been successfully observed. The main factor to increase the solution resistance may be obstruction of the ion transport due to the presence of gold nanoparticles. The existence of gold nanoparticles with 1.92 × 10(9) particles/mL in an indistinctly-colored chromatography paper is also identified by a solution resistance measurement. This indicates that the solution resistance assay has the potential to lower the detection limit of the conventional qualitative assay.
authors.affiliation: Department of Electrical Systems, Graduate School of Science and Engineering, Ritsumeikan University.
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Hori
authors.forename: Yuji
authors.initials: Y
authors.lastname: Harada
authors.forename: Tatsumi
authors.initials: T
authors.lastname: Kuretake
authors.forename: Shigeyasu
authors.initials: S
authors.lastname: Uno
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D002854:Chromatography, Paper / Q000295:instrumentation / Q000379:methods*; D003102:Colloids / Q000032:analysis* / Q000737:chemistry; D017097:Electric Impedance; D004867:Equipment Design; D006046:Gold / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry*; D010316:Particle Size; D011934:Reagent Strips
chemical_list: D003102:Colloids; D011934:Reagent Strips; D006046:Gold