title: [Validation Study for Analytical Method of Diarrhetic Shellfish Poisons in 9 Kinds of Shellfish].
abstract: A method was developed for the simultaneous determination of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 in shellfish using ultra performance liquid chromatography with tandem mass spectrometry. Shellfish poisons in spiked samples were extracted with methanol and 90% methanol, and were hydrolyzed with 2.5 mol/L sodium hydroxide solution. Purification was done on an HLB solid-phase extraction column. This method was validated in accordance with the notification of Ministry of Health, Labour and Welfare of Japan. As a result of the validation study in nine kinds of shellfish, the trueness, repeatability and within-laboratory reproducibility were 79-101%, less than 12 and 16%, respectively. The trueness and precision met the target values of notification.
authors.affiliation: Osaka Prefectural Institute of Public Health.
authors.forename: Mizuka
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Kensaku
authors.initials: K
authors.lastname: Kakimoto
authors.forename: Haruna
authors.initials: H
authors.lastname: Nagayoshi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okihashi
authors.forename: Keiji
authors.initials: K
authors.lastname: Kajimura
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D000432:Methanol; D019319:Okadaic Acid / Q000032:analysis / Q000031:analogs & derivatives; D011042:Poisons / Q000032:analysis*; D011714:Pyrans / Q000032:analysis; D015203:Reproducibility of Results; D012758:Shellfish / Q000032:analysis*; D057096:Shellfish Poisoning; D012972:Sodium Hydroxide; D012996:Solutions; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011042:Poisons; D011714:Pyrans; D012996:Solutions; D019319:Okadaic Acid; C051904:dinophysistoxin 1; D012972:Sodium Hydroxide; C076221:dinophysistoxin 2; D000432:Methanol