title: Phosphorylated testis-specific serine/threonine kinase 4 may phosphorylate Crem at Ser-117.
abstract: We aimed to investigate the internal existence status of testis-specific serine/threonine kinase 4 (Tssk4) and the interaction of Tssk4 and Cre-responsive element modulator (Crem). The internal existence status of Tssk4 in testis of mice was detected using western blotting and dephosphorylation method. The interaction of Tssk4 and Crem was analyzed by western blotting, immunohistochemistry, immunofluorescence, in vitro co-immunoprecipitation assays, and in vitro kinase assay. The results revealed that Tssk4 existed in testis both in phosphorylation and unphosphorylation status by a temporal manner with the development of testis. Immunofluorescence results showed that Tssk4 had identical distribution pattern with Crem in testis, which was utterly different to the localization of Cre-responsive element binding (Creb). In conclusion, our study demonstrated that phosphorylated Tssk4 might participate in testis genes expressions by phosphorylating Crem at Ser-117.
authors.affiliation: a State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics , Fudan University , Shanghai , P.R. China.
authors.forename: Guolong
authors.initials: G
authors.lastname: Fu
authors.affiliation: a State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics , Fudan University , Shanghai , P.R. China.
authors.forename: Youheng
authors.initials: Y
authors.lastname: Wei
authors.affiliation: b Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences , Wuhan University , Wuhan , P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: a State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics , Fudan University , Shanghai , P.R. China.
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D000818:Animals; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D051786:Cyclic AMP Response Element Modulator / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D010766:Phosphorylation; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D012694:Serine / Q000378:metabolism*; D015398:Signal Transduction; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
chemical_list: C497411:Crem protein, mouse; D051786:Cyclic AMP Response Element Modulator; D012694:Serine; D050882:CREB-Binding Protein; C494364:Crebbp protein, mouse; D017346:Protein Serine-Threonine Kinases; C529883:Tssk4 protein, mouse
keywords: Cre-responsive element binding protein; Cre-responsive element modulator; gene expression; phosphorylation; testis-specific serine/threonine kinase 4