title: Asymmetric Distribution of GFAP in Glioma Multipotent Cells.
abstract: Asymmetric division (AD) is a fundamental mechanism whereby unequal inheritance of various cellular compounds during mitosis generates unequal fate in the two daughter cells. Unequal repartitions of transcription factors, receptors as well as mRNA have been abundantly described in AD. In contrast, the involvement of intermediate filaments in this process is still largely unknown. AD occurs in stem cells during development but was also recently observed in cancer stem cells. Here, we demonstrate the asymmetric distribution of the main astrocytic intermediate filament, namely the glial fibrillary acid protein (GFAP), in mitotic glioma multipotent cells isolated from glioblastoma (GBM), the most frequent type of brain tumor. Unequal mitotic repartition of GFAP was also observed in mice non-tumoral neural stem cells indicating that this process occurs across species and is not restricted to cancerous cells. Immunofluorescence and videomicroscopy were used to capture these rare and transient events. Considering the role of intermediate filaments in cytoplasm organization and cell signaling, we propose that asymmetric distribution of GFAP could possibly participate in the regulation of normal and cancerous neural stem cell fate.
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 avenue Augustin Fliche, 34091 Montpellier Cedex 05, France.
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Guichet
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 avenue Augustin Fliche, 34091 Montpellier Cedex 05, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Guelfi
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 avenue Augustin Fliche, 34091 Montpellier Cedex 05, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Ripoll
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 avenue Augustin Fliche, 34091 Montpellier Cedex 05, France.
authors.forename: Marisa
authors.initials: M
authors.lastname: Teigell
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 avenue Augustin Fliche, 34091 Montpellier Cedex 05, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Sabourin
authors.affiliation: CHU Montpellier, Hopital Gui de Chaulliac, 80, avenue Augustin Fliche, 34295 Montpellier, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Bauchet
authors.affiliation: CHU Montpellier, Hopital Gui de Chaulliac, 80, avenue Augustin Fliche, 34295 Montpellier, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Rigau
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 avenue Augustin Fliche, 34091 Montpellier Cedex 05, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Rothhut
authors.affiliation: INSERM U1051, Institut des Neurosciences de Montpellier, Hôpital St Eloi, 80 avenue Augustin Fliche, 34091 Montpellier Cedex 05, France.|Université Montpellier, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Hugnot
mesh_terms: D000818:Animals; D060049:Asymmetric Cell Division; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015870:Gene Expression; D017930:Genes, Reporter; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008938:Mitosis; D039902:Multipotent Stem Cells / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism; D021381:Protein Transport
chemical_list: D005904:Glial Fibrillary Acidic Protein