title: In vitro assessment of the anthelmintic activity of Hedysarum carnosum Desf. at different phenological stages and from six locations in Tunisia.
abstract: Gastrointestinal nematodes are compromising productivity of grazing sheep and goats. Therefore, scientists have been looking for cost-effective alternative options. Forage legumes (Fabacea Family) contain tannins that could improve livestock performance and their health as well. The present study aimed to (i) determine the in vitro anthelmintic (AH) activity of 19 acetonic extracts of Hedysarum carnosum Desf on Haemonchus contortus by a larval exsheathment assay (LEA); (ii) test the anthelmintic activity of condensed tannins using a deactivating reagent, polyvinylpolypyrrolidone (PVPP); (iii) study the effect of location and the phenological stage on the percentage of exsheathment. The LEA was used at different concentrations (150, 300, 600, 1200 µg mL-1 of acetonic extract/mL of purified buffer solution (PBS)). The larval exsheathment is concentration, location, phenological stage dependent. All extracts, caused a delay of the percentage of exsheathment over 50% so the AH activity of H. carnosum was confirmed. After addition of PVPP, the % exsheathment was similar to the 150 µg mL-1 concentration. The biplot showed that Loc1(S), Loc4(B), Loc 5(PF), Loc 6(BM) and Loc 6(PF) were isolated from other plant extract sample. Our in vitro study showed that H. carnosum seems to be a promising alternative to AH drugs.
authors.affiliation: Institut Supérieur Agronomique de Chott-Mariem - Université de Sousse,BP 47,4042 Chott-Mariem,Tunisie.
authors.forename: A
authors.initials: A
authors.lastname: Aissa
authors.affiliation: UMR 1225 INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse - 23 Chemin des Capelles,31076 Toulouse Cedex,France.
authors.forename: F
authors.initials: F
authors.lastname: Manolaraki
authors.affiliation: International Center for Agricultural Research in the Dry Areas (ICARDA),DSIPS Program,11195 Amman,Jordan.
authors.forename: H
authors.initials: H
authors.lastname: Ben Salem
authors.affiliation: UMR 1225 INRA/ENVT, Ecole Nationale Vétérinaire de Toulouse - 23 Chemin des Capelles,31076 Toulouse Cedex,France.
authors.forename: H
authors.initials: H
authors.lastname: Hoste
authors.affiliation: Institut Supérieur Agronomique de Chott-Mariem - Université de Sousse,BP 47,4042 Chott-Mariem,Tunisie.
authors.forename: K
authors.initials: K
authors.lastname: Kraiem
mesh_terms: D000096:Acetone / Q000737:chemistry; D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D006189:Haemonchus / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011205:Povidone / Q000031:analogs & derivatives / Q000494:pharmacology; D014416:Tunisia
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts; D000096:Acetone; C077842:polyvinylpolypyrrolidone; D011205:Povidone
keywords: Hedysarum carnosum Desf; Tannins; larval exsheathment assay; location; nematodes