title: Molecular and biological characterization of highly infectious transcripts from full-length cDNA clones of broad bean wilt virus 1.
abstract: Broad bean wilt virus 1 (BBWV-1), genus Fabavirus, has a genome composed of two single-stranded positive-sense RNAs of ∼5.8 (RNA1) and 3.4kb (RNA2). Full-length cDNA clones of both genomic RNAs (pBenR1 and pBenR2) from BBWV-1 isolate Ben were constructed under the control of the T7 promoter. In vitro derived capped transcripts were infectious in Nicotiana benthamiana, Chenopodium quinoa and Vicia faba plants. The biological activity of viral transcripts was not affected by extra bases at the 5'-terminus introduced during in vitro transcription. Virions derived from the infectious cDNA clones displayed similar viral infectivity and accumulation, as well as symptom induction as the wild-type BBWV-1 isolate.
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain; Plant Pathology Department, University of California Davis, Davis, CA 95616, USA.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Ferriol
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Ambrós
authors.affiliation: CAPES Foundation, Ministry of Education of Brazil, Brasília, DF 70040-020, Brazil.
authors.forename: Dorivaldo M
authors.initials: DM
authors.lastname: da Silva
authors.affiliation: Plant Pathology Department, University of California Davis, Davis, CA 95616, USA.
authors.forename: Bryce W
authors.initials: BW
authors.lastname: Falk
authors.affiliation: Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain. Electronic address: lrubio@ivia.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Rubio
mesh_terms: D018076:DNA, Complementary; D004279:DNA, Viral; D019181:Fabavirus / Q000235:genetics / Q000472:pathogenicity*; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral; D014026:Nicotiana / Q000821:virology; D031307:Vicia faba / Q000821:virology; D014771:Virion / Q000235:genetics / Q000472:pathogenicity
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral
keywords: BBWV-1; Comovirinae; Fabavirus; Infectious clones; Secoviridae