title: [Effect of bone morphogenetic protein-2 on human heart valve interstitial cells calcification in vitro].
abstract: OBJECTIVE
  To establish human heart valve interstitial cells calcification culture model in vitro, and observe the effect of bone morphogenetic protein-2 (BMP-2) on calcification of human heart valve interstitial cells.
  
  
  METHODS
  Human heart valve interstitial cells were cultured in vitro, and divided into control group: cells were cultured in conventional media plus recombinant human BMP-2 treatment and experimental group: besides above treaments, calcification inducers ( recombinant human BMP-2, β-glycerophosphate, L-ascorbic acid, dexamethasone) were added to the culture media. The two group of cells were cultured for 14 days and were stained by Von Kossa, then the cell calcification was observed in this valvular interstitial cells calcification culture model in vitro. Protein expression of intercellular adhesion molecule 1 (ICAM-1), interleukin 8, BMP-2 and BMP-4 was determined by Western blot and BMP-2 secretion was measured by ELISA.
  
  
  RESULTS
  In the control group, the structure of human heart valve interstitial cells was clear, and the spindle and radial growth shaped cellular morphology was visible, and Von Kossa staining was negative. In the experimental group, the nuclei become darker in color, and granular sediment distribution was seen surrounding cells, and Von Kossa staining was positive, the cells were forming nodules of calcification. The protein expression of ICAM-1, interleukin 8, BMP-2 and BMP-4 in the experimental was significantly higher than that of the control group (all P < 0.05). The expression of BMP-2 in the experimental group was also significantly higher than that in control group ((92.5 ± 4.9) pg/ml vs. (22.2 ± 1.9) pg/ml, P < 0.05).
  
  
  CONCLUSIONS
  Human BMP-2, β-glycerophosphate, L-ascorbic acid, and dexamethasone can induce human heart valve interstitial cells calcification and enhance inflammation in vitro by stimulating the secretion of BMP-2.
authors.forename: Ling
authors.initials: L
authors.lastname: Qiuyang
authors.forename: Wu
authors.initials: W
authors.lastname: Ting
authors.forename: Ye
authors.initials: Y
authors.lastname: Ting
authors.forename: Su
authors.initials: S
authors.lastname: Zhaoliang
authors.forename: Zong
authors.initials: Z
authors.lastname: Gangjun
mesh_terms: D001205:Ascorbic Acid; D055396:Bone Morphogenetic Protein 2; D002114:Calcinosis; D002478:Cells, Cultured; D005994:Glycerophosphates; D006349:Heart Valve Diseases; D006801:Humans; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D005994:Glycerophosphates; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D001205:Ascorbic Acid; C031463:beta-glycerophosphoric acid