title: Cavernous nerve reconstruction with autologous vein graft and platelet-derived growth factors.
abstract: In this study, we investigated the feasibility of using autologous vein graft and platelet-derived growth factors to bridge transected cavernous nerve in a rat model. A short defect in the bilateral cavernous nerve was created and repaired with vein graft from the right jugular vein or vein graft plus platelet-derived growth factors. The 32 rats were divided into four groups, namely Group 1 - no repair as a negative control, Group 2 - vein graft alone, Group 3 - vein graft plus platelet-derived growth factors, and Group 4 - sham operation as a positive control. We evaluated nerve regeneration and functional recovery using retrograde tracing study with FluoroGold, Toluidine blue staining of cavernous nerve, and the intracavernous pressure at 3 months. Three months after surgery, rich FluoroGold-positive cells were observed in the sham and vein graft plus platelet-derived growth factors group, but very few were found in the no repair group. The number of myelinated axons of regenerated cavernous nerve and intracavernous pressure were increased obviously in the two vein graft groups, especially in the vein graft plus platelet-derived growth factors group. These findings confirm the feasibility of using autologous vein as guides for cavernous nerve regeneration, and the regeneration can be further enhanced when the vein is filled with platelet-derived growth factors.
authors.affiliation: Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Xie-Gang
authors.initials: XG
authors.lastname: Ding
authors.affiliation: Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Li
authors.affiliation: Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Zheng
authors.affiliation: Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Xing-Huan
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000818:Animals; D001369:Axons; D001808:Blood Vessels / Q000637:transplantation*; D002452:Cell Count; D007172:Erectile Dysfunction / Q000601:surgery; D005240:Feasibility Studies; D008297:Male; D009413:Nerve Fibers, Myelinated; D009416:Nerve Regeneration; D010410:Penile Erection / Q000502:physiology; D010413:Penis / Q000294:innervation / Q000601:surgery*; D010525:Peripheral Nerves / Q000601:surgery*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014182:Transplantation, Autologous
chemical_list: D010982:Platelet-Derived Growth Factor