title: Effect of Connexin43 on Mechanical Tension-stimulated Osteogenic Transcription Factors of Human Periodontal Ligament Fibroblasts.
abstract: OBJECTIVE
  To investigate the expression and effect of Connexin43 (Cx43) on tensile tension-stimulated osteogenic transcription factors of human periodontal ligament fibroblasts (hPDLFs).
  
  
  METHODS
  After hPDLFs were treated with 5% elongation tension for 1 h, 2 h, 4 h, 8 h, and 24 h, we examined the expressions of Cx43, Osterix, and RUNX2 at the mRNA level. After Cx43 expression was suppressed by siRNA or 18α-GA, the changes The mRNA in hPDLFs of Osterix and RUNX2 were observed.
  
  
  RESULTS
  The expressions of Cx43, Osterix, and RUNX2 mRNA in hPDLFs increased in a time-dependent fashion following tensile strain (all P<0.05), with the highest level at 5% elongation for 24 h. After Cx43 expression was blocked by two different methods, the increasing expressions of Osterix and RUNX2 were inhibited.
  
  
  CONCLUSIONS
  5% cyclic tension upregulates Cx43 expression and promotes the expression of Osterix and RUNX2 in a time-dependent manner. Cx43 may be involved in the osteogenic response of hPDLFs to mechanical tension.
authors.affiliation: Department of Stomatology,PUMC Hospital,CAMS and PUMC,Beijing100730,China.
authors.forename: Sheng-nan
authors.initials: SN
authors.lastname: Li
authors.affiliation: Department of Stomatology,PUMC Hospital,CAMS and PUMC,Beijing100730,China.
authors.forename: Hua-jing
authors.initials: HJ
authors.lastname: Zhang
authors.affiliation: Department of Biomechanics,School of Aerospace Engineering,Beijing Institute of Technology,Beijing 100081,China.
authors.forename: Bo
authors.initials: B
authors.lastname: Huo
authors.affiliation: Department of Stomatology,PUMC Hospital,CAMS and PUMC,Beijing100730,China.
authors.forename: Ding
authors.initials: D
authors.lastname: Zhang
mesh_terms: D002478:Cells, Cultured; D018031:Connexin 43; D005347:Fibroblasts; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives; D006801:Humans; D010012:Osteogenesis; D010513:Periodontal Ligament; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D013314:Stress, Mechanical; D014157:Transcription Factors; D015854:Up-Regulation
chemical_list: D018031:Connexin 43; C500216:GJA1 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014157:Transcription Factors; C119129:18alpha-glycyrrhetinic acid; D006034:Glycyrrhetinic Acid