title: Differential expression patterns of Nqo1, AKR1B8 and Ho-1 in the liver and small intestine of C57BL/6 mice treated with sulforaphane.
abstract: This data article contains complementary figures and results related to the research article entitled "butylated hydroxyanisole induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice" (Luo et al., 2015 [1]), which defined the basal and butylated hydroxyanisole (BHA)-induced expression patterns of Phase II enzymes Nqo1, AKR1B8, and Ho-1 in the liver and small intestine of C57BL/6 mice. Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)butane] (SFN), a naturally occurring isothiocyanate derived from cruciferous vegetables, is a highly potent inducer of phase II cytoprotective enzymes. This dataset reports the histological changes of Nqo1, AKR1B8, and Ho-1 in wild-type (WT) and Nrf2 (-/-) mice induced by SFN. The mice were given a 25 mg/kg single oral dose of SFN for 24 h and 48 h. Immunohistochemistry revealed that, in the liver from WT mice, SFN increased Nqo1 staining in hepatocytes with slight higher staining in the pericentral region. The induction of AKR1B8 appeared mostly in hepatocytes in the periportal region. The basal and inducible Ho-1 was located predominately in Kupffer cells. In the small intestine from WT mice, the inducible expression of Nqo1 and AKR1B8 appeared more obvious in the villus than that in the crypt.
authors.affiliation: Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, PR China; Department of Pharmacology, University of Nantong, Nantong, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yeru
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Deqi
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Shou
authors.affiliation: Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Shengcun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ye
authors.affiliation: Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Jun Wang
keywords: AKR1B8; HO-1; NQO1; Nrf2; Sulforaphane