title: Tianeptine, olanzapine and fluoxetine show similar restoring effects on stress induced molecular changes in mice brain: An FT-IR study.
abstract: Chronic stress which can cause a variety of disorders and illness ranging from metabolic and cardiovascular to mental leads to alterations in content, structure and dynamics of biomolecules in brain. The determination of stress-induced changes along with the effects of antidepressant treatment on these parameters might bring about more effective therapeutic strategies. In the present study, we investigated unpredictable chronic mild stress (UCMS)-induced changes in biomolecules in mouse brain and the restoring effects of tianeptine (TIA), olanzapine (OLZ) and fluoxetine (FLX) on these variations, by Fourier transform infrared (FT-IR) spectroscopy. The results revealed that chronic stress causes different membrane packing and an increase in lipid peroxidation, membrane fluidity. A significant increment for lipid/protein, C=O/lipid, CH3/lipid, CH2/lipid, PO(-)2/lipid, COO(-)/lipid and RNA/protein ratios but a significant decrease for lipid/protein ratios were also obtained. Additionally, altered protein secondary structure components were estimated, such as increment in random coils and beta structures. The administration of TIA, OLZ and FLX drugs restored these stress-induced variations except for alterations in protein structure and RNA/protein ratio. This may suggest that these drugs have similar restoring effects on the consequences of stress activity in brain, in spite of the differences in their action mechanisms. All findings might have importance in understanding molecular mechanisms underlying chronic stress and contribute to studies aimed for drug development.
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, 41380, Kocaeli, Turkey. Electronic address: sevgitrkr@gmail.com.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Türker-Kaya
authors.affiliation: Department of Pharmacology, Faculty of Medicine, 41380, Kocaeli, Turkey.
authors.forename: Oğuz
authors.initials: O
authors.lastname: Mutlu
authors.affiliation: Department of Pharmacology, Faculty of Medicine, 41380, Kocaeli, Turkey.
authors.forename: İpek K
authors.initials: İK
authors.lastname: Çelikyurt
authors.affiliation: Department of Pharmacology, Faculty of Medicine, 41380, Kocaeli, Turkey.
authors.forename: Furuzan
authors.initials: F
authors.lastname: Akar
authors.affiliation: Department of Pharmacology, Faculty of Medicine, 41380, Kocaeli, Turkey.
authors.forename: Güner
authors.initials: G
authors.lastname: Ulak
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000627:therapeutic use*; D001569:Benzodiazepines / Q000494:pharmacology* / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects*; D005473:Fluoxetine / Q000494:pharmacology* / Q000627:therapeutic use; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D000077152:Olanzapine; D013315:Stress, Psychological / Q000188:drug therapy* / Q000378:metabolism; D013841:Thiazepines / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000928:Antidepressive Agents; D013841:Thiazepines; D005473:Fluoxetine; C050504:tianeptine; D001569:Benzodiazepines; D000077152:Olanzapine
keywords: Biomolecules; Brain; FT-IR; Fluoxetine; Olanzapine; Stress; Tianeptine; UCMS