title: Cellular Engineering with Membrane Fusogenic Liposomes to Produce Functionalized Extracellular Vesicles.
abstract: Engineering of extracellular vesicles (EVs) without affecting biological functions remains a challenge, limiting the broad applications of EVs in biomedicine. Here, we report a method to equip EVs with various functional agents, including fluorophores, drugs, lipids, and bio-orthogonal chemicals, in an efficient and controlled manner by engineering parental cells with membrane fusogenic liposomes, while keeping the EVs intact. As a demonstration of how this method can be applied, we prepared EVs containing azide-lipids, and conjugated them with targeting peptides using copper-free click chemistry to enhance targeting efficacy to cancer cells. We believe that this liposome-based cellular engineering method will find utility in studying the biological roles of EVs and delivering therapeutic agents through their innate pathway.
authors.affiliation: Department of Bio and Brain Engineering, §Graduate School of Medical Science and Engineering, ⊥Institute for Health Science and Technology, and ∥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Junsung
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Bio and Brain Engineering, §Graduate School of Medical Science and Engineering, ⊥Institute for Health Science and Technology, and ∥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Hyoungjin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Bio and Brain Engineering, §Graduate School of Medical Science and Engineering, ⊥Institute for Health Science and Technology, and ∥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Unbyeol
authors.initials: U
authors.lastname: Goh
authors.affiliation: Department of Bio and Brain Engineering, §Graduate School of Medical Science and Engineering, ⊥Institute for Health Science and Technology, and ∥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Bio and Brain Engineering, §Graduate School of Medical Science and Engineering, ⊥Institute for Health Science and Technology, and ∥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Moonkyoung
authors.initials: M
authors.lastname: Jeong
authors.affiliation: Department of Bio and Brain Engineering, §Graduate School of Medical Science and Engineering, ⊥Institute for Health Science and Technology, and ∥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Jean
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Bio and Brain Engineering, §Graduate School of Medical Science and Engineering, ⊥Institute for Health Science and Technology, and ∥Institute for the Nanocentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
authors.forename: Ji-Ho
authors.initials: JH
authors.lastname: Park
mesh_terms: D060846:Cell Engineering / Q000379:methods*; D045744:Cell Line, Tumor; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*
chemical_list: D008081:Liposomes
keywords: cellular engineering; click chemistry; drug delivery; extracellular vesicle; liposome