title: [Inflammasome responses in macrophages induced by C-terminal peptides of Escherichia coli EscI protein].
abstract: OBJECTIVE
  To analyze NLRC4 inflammasome responses in macrophages induced by C-terminal of Escherichia coli EscI protein.
  
  
  METHODS
  NLRC4 inflammasome responses in mouse peritoneal macrophages were analyzed after delivery of the peptides containing C-terminal amino acid sequences of E. coli EscI protein in vitro.
  
  
  RESULTS
  The peptides containing C-terminal 15 amino acids of EscI protein could significantly activate NLRC4 inflammasome responses in macrophages pre-stimulated with lipopolysaccharide. Intracellular caspase-1 was activated and pyroptotic dead cells were found after peptides delivery. The contents of cytokines, IL-1β and IL-18, in supernatants were elevated significantly compared with that of the control (P < 0.05). Besides, through comparison of IL-1β contents under different stimulation conditions, 4 h incubation after peptides delivery (peptides: lipofectamine 2000 = 70 μg/μL) could obviously promote the secretion of IL-1β.
  
  
  CONCLUSIONS
  Peptides containing C-terminal 15 amino acids of E. coli EscI protein can significantly induce NLRC4 inflammasome activation in macrophages.
authors.forename: Maozhi
authors.initials: M
authors.lastname: Hu
authors.forename: Wenhua
authors.initials: W
authors.lastname: Li
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Yan
authors.forename: Shizhong
authors.initials: S
authors.lastname: Geng
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.forename: Guiyoui
authors.initials: G
authors.lastname: Cui
authors.forename: Xin'an
authors.initials: X
authors.lastname: Jiao
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D006801:Humans; D058847:Inflammasomes / Q000235:genetics / Q000276:immunology*; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: C570860:EscI protein, E coli; D029968:Escherichia coli Proteins; D058847:Inflammasomes; D020382:Interleukin-18; D053583:Interleukin-1beta