title: Molecular cloning, expression of, and regulation by thyroid-hormone receptor α A in the half-smooth tongue sole Cynoglossus semilaevis during metamorphosis.
abstract: To elucidate the effect of thyroid hormone receptor α A (thraa) on metamorphosis, the full length cDNA of half-smooth tongue sole Cynoglossus semilaevis was cloned. The relative gene transcript level of thraa at different development stages was quantified using real-time PCR. Transcription of thraa increased and declined rapidly during metamorphosis. Hyperthyroidism was induced in juveniles and larvae with exposure to T3 and T4, and hypothyroidism with thiourea (TU), 2-mercapto-1-methylimidazole (MMI). thraa mRNA was higher in fish treated for 6 days with MMI than in untreated controls, although inhibited larvae did not complete metamorphosis. The addition of exogenous T4 reversed this effect in the MMI-treated group, but not in the TU-treated group. In situ hybridization revealed progressive tail end of body growth and change during developmental stages, with corresponding changes in thraa expression. This process may be induced by thyroid hormones with thraa as a major mediator. The morphological changes of tip of the tail may be associated with the development of lateral swimming.
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|College of Fisheries and Life Science, Dalian Ocean University, Dalian, Liaoning 116023, China.
authors.forename: W T
authors.initials: WT
authors.lastname: Zhang
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|College of Fisheries and Life Science, Shanghai Ocean University, Yangpu, Shanghai 201306, China.
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|College of Fisheries and Life Science, Shanghai Ocean University, Yangpu, Shanghai 201306, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Xiang
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|College of Fisheries and Life Science, Dalian Ocean University, Dalian, Liaoning 116023, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|College of Fisheries and Life Science, Shanghai Ocean University, Yangpu, Shanghai 201306, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|School of Marine life Sciences, Ocean University of China, Qingdao, Shandong 266003, China.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Dong
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Li
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Li
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
authors.affiliation: Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.|Function Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Ocean Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 26607, China.
authors.forename: N
authors.initials: N
authors.lastname: Wang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005412:Flatfishes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007093:Imidazoles; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008675:Metamorphosis, Biological / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013963:Thyroid Hormones / Q000378:metabolism / Q000502:physiology*
chemical_list: C107674:2-mercapto-1-methylimidazole; D007093:Imidazoles; D012333:RNA, Messenger; D013963:Thyroid Hormones
keywords: drug treatment; hyperthyroidism; hypothyroidism; tail end; whole-mount in situ hybridization