title: UCP1 and UCP3 Expression Is Associated with Lipid and Carbohydrate Oxidation and Body Composition.
abstract: OBJECTIVE
  Uncoupling proteins (UCPs) are located in the inner membrane of mitochondria. These proteins participate in thermogenesis and energy expenditure. This study aimed to evaluate how UCP1 and UCP3 expression influences substrate oxidation and elicits possible changes in body composition in patients submitted to bariatric surgery.
  
  
  METHODS
  This is a longitudinal study comprising 13 women with obesity grade III that underwent bariatric surgery and 10 healthy weight individuals (control group). Body composition was assessed by bioelectrical impedance. Carbohydrate and fat oxidation was determined by indirect calorimetry. Subcutaneous adipose tissue was collected for gene expression analysis. QPCR was used to evaluate UCP1 and UCP3 expression.
  
  
  RESULTS
  Obese patients and the control group differed significantly in terms of lipid and carbohydrate oxidation. Six months after bariatric surgery, the differences disappeared. Lipid oxidation correlated with the percentage of fat mass in the postoperative period. Multiple linear regression analysis showed that the UCP1 and UCP3 genes contributed to lipid and carbohydrate oxidation. Additionally, UCP3 expression was associated with BMI, percentage of lean body mass, and percentage of mass in the postoperative period.
  
  
  CONCLUSIONS
  UCP1 and UCP3 expression is associated with lipid and carbohydrate oxidation in patients submitted to bariatric surgery. In addition, UCP3 participates in body composition modulation six months postoperatively.
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Bruno A P
authors.initials: BA
authors.lastname: Oliveira
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Marcela A S
authors.initials: MA
authors.lastname: Pinhel
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Carolina F
authors.initials: CF
authors.lastname: Nicoletti
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Cristiana C
authors.initials: CC
authors.lastname: Oliveira
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Driele C G
authors.initials: DC
authors.lastname: Quinhoneiro
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Natália Y
authors.initials: NY
authors.lastname: Noronha
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Júlio S
authors.initials: JS
authors.lastname: Marchini
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Ana J
authors.initials: AJ
authors.lastname: Marchry
authors.affiliation: Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Wilson S
authors.initials: WS
authors.lastname: Junior
authors.affiliation: Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
authors.forename: Carla B
authors.initials: CB
authors.lastname: Nonino
mesh_terms: D050154:Adiposity; D000328:Adult; D000886:Anthropometry; D050110:Bariatric Surgery; D001823:Body Composition; D015992:Body Mass Index; D050260:Carbohydrate Metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D050356:Lipid Metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D000071256:Uncoupling Protein 1; D000071247:Uncoupling Protein 3
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604371:UCP1 protein, human; C000604392:UCP3 protein, human; D000071256:Uncoupling Protein 1; D000071247:Uncoupling Protein 3