title: Protein-binding, cytotoxicity in vitro and cell cycle arrest of ruthenium(II) polypyridyl complexes.
abstract: The cytotoxic activity of two Ru(II) complexes against A549, BEL-7402, HeLa, PC-12, SGC-7901 and SiHa cell lines was investigated by MTT method. Complexes 1 and 2 show moderate cytotoxicity toward BEL-7402 cells with an IC50 value of 53.9 ± 3.4 and 39.3 ± 2.1 μM. The effects of the complexes inducing apoptosis, cellular uptake, reactive oxygen species and mitochondrial membrane potential in BEL-7402 cells have been studied by fluorescence microscopy. The percentages of apoptotic and necrotic cells and cell cycle arrest were studied by flow cytometry. The BSA-binding behaviors were investigated by UV/visible and fluorescent spectra.
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Si-Hong
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Liang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Gui-Qiang
authors.initials: GQ
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Di-Hua
authors.initials: DH
authors.lastname: Cao
authors.affiliation: Department of Pharmacy, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Yang-Yang
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China. Electronic address: wuyong@gzhmu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China. Electronic address: qfguo@gzhmu.edu.cn.
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Guo
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009369:Neoplasms / Q000188:drug therapy / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D017382:Reactive Oxygen Species; D015082:2,2'-Dipyridyl; D012428:Ruthenium
keywords: Apoptosis; BSA-binding; Cell cycle arrest; Cytotoxicity in vitro; Ru(II) complexes