title: Oral Immunization of Rabbits with S. enterica Typhimurium Expressing Neisseria gonorrhoeae Filamentous Phage Φ6 Induces Bactericidal Antibodies Against N. gonorrhoeae.
abstract: All Neisseria gonorrhoeae strains whose DNA sequences have been determined possess filamentous phage DNA sequences. To ascertain if phage encoded proteins could form the basis of a gonococcal vaccine, rabbits were orally infected with S. enterica Typhimurium strain χ3987 harboring phagemid NgoΦ6 fm. The elicited sera contained large quantities of anti-phage IgG and IgA antibodies that bound to the surface of N. gonorrhoeae cells, as shown by indirect fluorescent analysis and flow cytometry. The elicited sera was able to bind to several phage proteins. The sera also had bactericidal activity. These data demonstrate that N. gonorrhoeae filamentous phage can induce antibodies with anti-gonococcal activity and that phage proteins may be a candidate for vaccine development.
authors.affiliation: Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Piekarowicz
authors.affiliation: Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Aneta
authors.initials: A
authors.lastname: Kłyż
authors.affiliation: Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Majchrzak
authors.affiliation: University of Maryland, Department of Cell Biology and Molecular Genetics, College Park, MD 20742, USA.
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Stein
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D001433:Bacteriolysis / Q000235:genetics; D017126:Bacteriophage phi 6 / Q000235:genetics*; D006069:Gonorrhea / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D007114:Immunization; D017912:Inovirus / Q000235:genetics*; D009344:Neisseria gonorrhoeae / Q000276:immunology / Q000821:virology*; D011817:Rabbits; D012485:Salmonella typhi / Q000235:genetics / Q000276:immunology* / Q000821:virology; D058248:Serum Bactericidal Antibody Assay
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines