title: The Use of Kits in the Analysis of Tissue Lipids Requires Validation.
abstract: The ready availability and ease of use of kits for the measurement of serum lipids has greatly facilitated these measurements. In many cases it would be convenient to use these kits in the determination of lipid concentrations in tissues. The successful application of serum kits in tissue analysis requires that two important issues be considered. First, the solvent system for the extraction of the lipids and the solvent used for analysis by the kit must be compatible with the reactions in the kit. Second, the concentration range in the analyzed solution must be within the range for which the kit is used. We report here that lipids in liver and adipose tissues may be significantly underestimated by the use of some kits. We recommend that the use of kits for tissue analysis of lipids be validated for the specific analysis.
authors.affiliation: Department of Cancer Biology, University of Cincinnati, Cincinnati, USA.
authors.forename: T
authors.initials: T
authors.lastname: Rider
authors.affiliation: Department of Medicine, University of Washington, Seattle, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: LeBoeuf
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, USA. Patrick.Tso@uc.edu.
authors.forename: Patrick
authors.initials: P
authors.lastname: Tso
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Jandacek
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D051379:Mice; D011933:Reagent Kits, Diagnostic; D012997:Solvents / Q000737:chemistry; D054928:Validation Studies as Topic
chemical_list: D008055:Lipids; D011933:Reagent Kits, Diagnostic; D012997:Solvents
keywords: Analytical biochemistry; Lipid analysis; Phospholipid analysis; Triglyceride analysis