title: A Sensitive and Disposable Graphene Oxide Electrochemical Immunosensor for Label-free Detection of Human Immunoglobulin G.
abstract: In this work, a cost-effective and simple-to-prepare label-free electrochemical immunosensor was, for the first time, fabricated by modifying high-quality graphene oxide (GPO) onto a screen-printed carbon electrode (SPCE). The anti-IgG antibody was then covalently immobilized to the carboxylic group anchoring on the surface of GPO particles. Under the optimized condition, our newly developed immunosensor selectively bound to human immunoglobulin G (IgG), a model biomarker, with high sensitivity at a limit of detection of 1.99 ng mL(-1), potentially sensitive enough for IgG detection at the pathophysiological level, and had a linear range of 2.5 to 100 ng mL(-1). The proposed immunosensor also exhibited high reproducibility and regenerability, resulting in no significant change in electrochemical signals from different replicates of the electrode, and a robust electrochemical current after being subjected to alkaline base washing with several cycles. To this end, our immunosensor demonstrates ability as a promising diagnostic tool for clinical assessment.
authors.affiliation: Department of Chemistry, Faculty of Science, Chiang Mai University.
authors.forename: Watthanachai
authors.initials: W
authors.lastname: Jumpathong
authors.forename: Jaroon
authors.initials: J
authors.lastname: Jakmunee
authors.forename: Kontad
authors.initials: K
authors.lastname: Ounnunkad
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004579:Electron Transport; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D010087:Oxides; D006108:Graphite