title: Effect of ozone on colon anastomoses in rat peritonitis model.
abstract: OBJECTIVE
  To investigate the effects of medical ozone theraphy on the colon anastomosis of peritonitis model in rats.
  
  
  METHODS
  Eighteen rats were randomly assigned into three equal groups; control, cecal punctuation and colon anastomosis and ozone theraphy. Sepsis was performed with a cecal punctuation in groups 2 and 3. The medical ozone theraphy was administered intraperitonealy for three weeks in group 3 while the other rats received saline injection. At the twenty second day serum were obtained for TNF-α and IL-1β, the colonic burst pressures were measured and colonic tissue samples were obtained for MDA and MPO levels. Histolopatological examination was evaluated with H&E stain, and Ki-67, IL-1β and the VEGF immunostaining densities were also compared.
  
  
  RESULTS
  Intraperitoneal ozone administration reversed TNF-α, IL-1β, MDA and MPO levels and the colonic burst pressures. There was also a significant difference at immunostaining densities of histopathological examination.
  
  
  CONCLUSIONS
  Medical ozone therapy may contribute to tissue healing by affecting the proliferation and the vascularization thus has benefits on colonic anastomosis at peritonitis in rats.
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Tuğrul
authors.initials: T
authors.lastname: Çakır
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Arif
authors.initials: A
authors.lastname: Aslaner
authors.affiliation: Department of Biochemistry, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Seçkin Özgür
authors.initials: SÖ
authors.lastname: Tekeli
authors.affiliation: Department of Histology and Embryology, Akdeniz University, Antalya, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Avcı
authors.affiliation: Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Doğan
authors.affiliation: Department of Biochemistry, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Feyza
authors.initials: F
authors.lastname: Tekeli
authors.affiliation: Department of Histology and Embryology, Akdeniz University, Antalya, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Soylu
authors.affiliation: Department of Gastroenterology, Haydarpaşa Training and Research Hospital, Istanbul, Turkey.
authors.forename: Cebrail
authors.initials: C
authors.lastname: Akyüz
authors.affiliation: Department of General Surgery, Faculty of Medicine, Inonü University, Malatya, Turkey.
authors.forename: Süleyman
authors.initials: S
authors.lastname: Koç
authors.affiliation: Department of Histology and Embryology, Akdeniz University, Antalya, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Üstünel
authors.affiliation: Department of Biochemistry, Antalya Training and Research Hospital, Antalya, Turkey.
authors.forename: Necat
authors.initials: N
authors.lastname: Yılmaz
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000473:pathology / Q000601:surgery*; D004195:Disease Models, Animal; D053583:Interleukin-1beta / Q000032:analysis / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D010126:Ozone / Q000494:pharmacology*; D010538:Peritonitis / Q000139:chemically induced*; D009195:Peroxidase / Q000032:analysis / Q000187:drug effects; D011897:Random Allocation; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D010126:Ozone; D009195:Peroxidase