title: Mitochondrial ribosomal protein S18-2 is highly expressed in endometrial cancers along with free E2F1.
abstract: Endometrial cancer (EC) is one of the most frequent causes of cancer death among women in developed countries. Histopathological diagnosis and imaging techniques for EC are limited, thus new prognostic markers are needed to offer patients the best treatment and follow-up.In the present paper we showed that the level of mitochondrial ribosomal protein MRPS18-2 (S18-2) increased in EC compared with the normal endometrium and hyperplasia, based on a study of 42 patient biopsies. Importantly, high expression of free E2F1 in EC correlates well with high S18-2 expression. The EC cell line HEC-1-A, which overexpresses S18-2 constitutively, showed an increased proliferation capacity in vitro and in vivo (in SCID mice). Moreover, pan-keratin, beta-catenin and E-cadherin signals are diminished in these cells, compared to the parental HEC-1-A line, in contrast to vimentin signal that is increased. This may be associated with epithelial-mesenchymal cell transition (EMT).We conclude that high expression of S18-2 and free E2F1, and low pan-keratin, beta-catenin, and E-cadherin signals might be a good set of prognostic markers for EC.
authors.affiliation: Department of Women's and Children's Health (KBH), Karolinska Institutet, Stockholm, 17176, Sweden.
authors.forename: Miriam
authors.initials: M
authors.lastname: Mints
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, 17177, Sweden.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Mushtaq
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, 03022, Ukraine.
authors.forename: Natalia
authors.initials: N
authors.lastname: Iurchenko
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, 03022, Ukraine.
authors.forename: Larysa
authors.initials: L
authors.lastname: Kovalevska
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, 17177, Sweden.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Stip
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, 03022, Ukraine.
authors.forename: Daria
authors.initials: D
authors.lastname: Budnikova
authors.affiliation: Department of Women's and Children's Health (KBH), Karolinska Institutet, Stockholm, 17176, Sweden.
authors.forename: Sonia
authors.initials: S
authors.lastname: Andersson
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, 03022, Ukraine.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Polischuk
authors.affiliation: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, 03022, Ukraine.
authors.forename: Lubov
authors.initials: L
authors.lastname: Buchynska
authors.affiliation: Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, 17177, Sweden.|R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, 03022, Ukraine.
authors.forename: Elena
authors.initials: E
authors.lastname: Kashuba
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D015703:Antigens, CD; D014408:Biomarkers, Tumor / Q000032:analysis*; D015820:Cadherins / Q000096:biosynthesis; D050687:E2F1 Transcription Factor / Q000096:biosynthesis*; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D064593:Heterografts; D006801:Humans; D007633:Keratins / Q000096:biosynthesis; D051379:Mice; D016513:Mice, SCID; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D051176:beta Catenin / Q000096:biosynthesis
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C513384:CDH1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D012269:Ribosomal Proteins; D051176:beta Catenin; C041184:ribosomal protein S18; D007633:Keratins
keywords: E2F1; cell transformation; endometrial cancer; mitochondrial ribosomal protein MRPS18-2; prognostic markers