title: In depth analysis of the Sox4 gene locus that consists of sense and natural antisense transcripts.
abstract: SRY (Sex Determining Region Y)-Box 4 or Sox4 is an important regulator of the pan-neuronal gene expression during post-mitotic cell differentiation within the mammalian brain. Sox4 gene locus has been previously characterized with multiple sense and overlapping natural antisense transcripts [1], [2]. Here we provide accompanying data on various analyses performed and described in Ling et al. [2]. The data include a detail description of various features found at Sox4 gene locus, additional experimental data derived from RNA-Fluorescence in situ Hybridization (RNA-FISH), Western blotting, strand-specific reverse-transcription quantitative polymerase chain reaction (RT-qPCR), gain-of-function and in situ hybridization (ISH) experiments. All the additional data provided here support the existence of an endogenous small interfering- or PIWI interacting-like small RNA known as Sox4_sir3, which origin was found within the overlapping region consisting of a sense and a natural antisense transcript known as Sox4ot1.
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia; NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia.
authors.forename: King-Hwa
authors.initials: KH
authors.lastname: Ling
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Brautigan
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Moore
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia.
authors.forename: Rachel
authors.initials: R
authors.lastname: Fraser
authors.affiliation: NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia.
authors.forename: Melody Pui-Yee
authors.initials: MP
authors.lastname: Leong
authors.affiliation: NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia.
authors.forename: Jia-Wen
authors.initials: JW
authors.lastname: Leong
authors.affiliation: NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia.
authors.forename: Shahidee
authors.initials: S
authors.lastname: Zainal Abidin
authors.affiliation: NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia.
authors.forename: Han-Chung
authors.initials: HC
authors.lastname: Lee
authors.affiliation: NeuroBiology & Genetics Group, Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor DE, Malaysia; School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia; Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor DE, Malaysia.
authors.forename: Pike-See
authors.initials: PS
authors.lastname: Cheah
authors.affiliation: School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Joy M
authors.initials: JM
authors.lastname: Raison
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia.
authors.forename: Milena
authors.initials: M
authors.lastname: Babic
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia.
authors.forename: Young Kyung
authors.initials: YK
authors.lastname: Lee
authors.affiliation: School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Tasman
authors.initials: T
authors.lastname: Daish
authors.affiliation: Theme of Laboratory and Community Genetics, Murdoch Childrens Research Institute, Royal Children׳s Hospital, Flemington Road, Parkville, VIC 3052, Australia.
authors.forename: Deidre M
authors.initials: DM
authors.lastname: Mattiske
authors.affiliation: Biomedicine Discovery Institute, Monash University, VIC 3800, Australia.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Mann
authors.affiliation: School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: David L
authors.initials: DL
authors.lastname: Adelson
authors.affiliation: School of Biological Sciences, Faculty of Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Paul Q
authors.initials: PQ
authors.lastname: Thomas
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia.
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Hahn
authors.affiliation: Department of Molecular Pathology, The Institute of Medical and Veterinary Science and The Hanson Institute, P.O. Box 14 Rundle Mall Post Office, Adelaide, SA 5000, Australia; School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Hamish S
authors.initials: HS
authors.lastname: Scott
keywords: Brain development; Endogenous siRNA; Natural antisense transcripts