title: New Small Plasmid Harboring blaKPC-2 in Pseudomonas aeruginosa.
abstract: The aim of this study was to characterize the genetic context of blaKPC-2 in Pseudomonas aeruginosa sequence type 244 from Brazil. The blaKPC-2 gene was detected in a new small plasmid, pBH6. Complete sequencing revealed that pBH6 was 3,652 bp long and included the Tn3 resolvase and Tn3 inverted repeat (IR), a partial copy of ISKpn6, and a putative ori region but no rep genes. pBH6 replicated stably into Escherichia coli strain DH10B and P. aeruginosa strain PAO.
authors.affiliation: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil regaletti@hotmail.com.
authors.forename: Renata
authors.initials: R
authors.lastname: Galetti
authors.affiliation: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil.
authors.forename: Leonardo Neves
authors.initials: LN
authors.lastname: Andrade
authors.affiliation: Laboratoire de Microbiologie et Génétique Moléculaires, Centre National de la Recherche Scientifique, Toulouse, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Chandler
authors.affiliation: Faculdade de Ciencias Agrarias e Veterinarias, Universidade Estadual Paulista Julio de Mesquita Filho, Jaboticabal, Brazil.
authors.forename: Alessandro de Mello
authors.initials: Ade M
authors.lastname: Varani
authors.affiliation: Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil.
authors.forename: Ana Lúcia Costa
authors.initials: AL
authors.lastname: Darini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D004269:DNA, Bacterial / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial