title: Fabrication of a sensitive amperometric sensor for NADH and H2O2 using palladium nanoparticles-multiwalled carbon nanotube nanohybrid.
abstract: Palladium nanoparticles decorated multiwalled carbon nanotubes (PdNPs-MWCNTs) were synthesized and simply cast on the surface of a glassy carbon electrode (GCE) to prepare an amperometric sensor. The fabricated sensor (PdNPs-MWCNTs/GCE) showed excellent electrocatalytic activity towards NADH and H2O2 oxidation and H2O2 reduction. A fast, linear and highly sensitive response was observed for NADH in the concentration range between 0.1 and 200 μM with a detection limit (S/N=3) of 32 nM. Also, the sensor exhibited fast and sensitive responses (<2 s) towards H2O2. The sensitivity and detection limit for H2O2 at the operating potential of +0.35 V were 167 nA μM(-1)cm(-2) and 1.2 μM, respectively and better than those obtained at the operating potential of -0.25 V (68 nA μM(-1)cm(-2) and 14 μM). Moreover, further modification of the proposed sensor by glucose oxidase led to the fabrication of a glucose biosensor with satisfactory performance.
authors.affiliation: Department of Chemistry, Zanjan Branch, Islamic Azad University, P.O. Box 49195-467, Zanjan, Iran. Electronic address: hamidi.h@hotmail.com.
authors.forename: Hassan
authors.initials: H
authors.lastname: Hamidi
authors.affiliation: Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran; Department of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box 45195-1159, Gava Zang, Zanjan, Iran. Electronic address: bhaghighi@shirazu.ac.ir.
authors.forename: Behzad
authors.initials: B
authors.lastname: Haghighi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009243:NAD / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D009243:NAD; D010165:Palladium; D006861:Hydrogen Peroxide
keywords: Electrocatalysis, NADH; Glucose biosensor; Hydrogen peroxide; Multiwalled carbon nanotubes; Palladium nanoparticles