title: Development of a sensitive monoclonal-based enzyme-linked immunosorbent assay for monitoring T-2 toxin in food and feed.
abstract: The consumption of food or feed contaminated with high levels of T-2 toxin may cause adverse health effects in humans and other animals. In this study, to monitor T-2 toxin rapidly in food and feed, a sensitive and specific monoclonal antibody (mAb) against T-2 toxin was generated and a simple and rapid indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) developed. T-2 toxin was first converted to T-2-hemisuccinate (T-2HS) and T-2-hemiglutarate (T-2HG), which were then conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to prepare an immunogen and coating antigen, respectively. After the inoculation of female Balb/c mice and cell fusions, one cell line, 4D8, with the IgG1 isotype was obtained. The 4D8 antibody exhibited the ability specifically to recognise T-2 toxin with IC50 1.46 µg l(-1). Based on this 4D8 mAb, an optimised ic-ELISA protocol was developed using only methanol-water (7:3, v/v) in feed and cereal samples and ethyl acetate in muscle samples. The limits of detection of T-2 toxin in various sample matrices varied from 0.07 to 15.8 µg kg(-1); the recoveries ranged from 50.3% to 113.6%; and the CVs were less than 19.0%. These results suggest that the prepared mAb and the developed ic-ELISA method will be a useful tool for detecting T-2 toxin in foods and feeds.
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Peng
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Chang
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Yulian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Chen
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.affiliation: a National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety , Huazhong Agricultural University , Wuhan , Hubei , China.
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D013605:T-2 Toxin / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D013605:T-2 Toxin
keywords: T-2 toxin; feed; food; indirect competitive enzyme-linked immunosorbent assay; monoclonal antibody