title: Role of oxidative stress in the pathophysiology of Toxoplasma gondii infection.
abstract: Oxidative stress (OS) plays an essential role in the pathogenesis of common neurodegenerative diseases. We have previously shown that Toxoplasma gondii (T. gondii) induces high nitric oxide (NO) production, glial activation, and apoptosis that altogether cause severe neuropathology in toxoplasma encephalitis (TE). The objective of this study was to investigate the cytotoxic effect of OS and to identify a correlation between the causes of T. gondii induced neuropathology. Expression levels of glutathione reductase (GR), Cu/Zn superoxide dismutase (SOD1), neuron specific enolase (NSE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were investigated. Results of the study revealed that the levels of GR (P <0.005) and NSE (P <0.001) expression in the brain tissue markedly increased while SOD1 activity decreased (P <0.001) in the infected group compared to the non-infected group. In addition, intense staining for 8-OHdG (P <0.05) was observed both in the nucleus and the cytoplasm of neurons and glial cells that underwent OS. These results were reasonable to suggest that T. gondii-mediated OS might play a pivotal role and a different type of role in the mechanism of neurodegeneration/neuropathology in the process of TE. The results also clearly indicated that increased levels of NO and apoptosis might contribute to OS-related pathogenesis of TE. As a result, OS and expression of NSE might give an idea of the disease progress and may have a critical diagnostic significance for patients with T. gondii infection.
authors.affiliation: Gumushane University, Siran Mustafa Beyaz Vocational High School, Siran, Gumushane, Turkey gcdincel@yahoo.com.tr.
authors.forename: Gungor Cagdas
authors.initials: GC
authors.lastname: Dincel
authors.affiliation: Kirikkale University, Faculty of Veterinary Medicine, Department of Pathology, Yahsihan, Kirikkale, Turkey.
authors.forename: Hasan Tarik
authors.initials: HT
authors.lastname: Atmaca
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000469:parasitology; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005980:Glutathione Reductase / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D009457:Neuroglia / Q000378:metabolism / Q000469:parasitology; D009474:Neurons / Q000378:metabolism / Q000469:parasitology; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D000072105:Superoxide Dismutase-1 / Q000378:metabolism; D014122:Toxoplasma / Q000472:pathogenicity*; D016781:Toxoplasmosis, Cerebral / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology*
chemical_list: D009569:Nitric Oxide; D007371:Interferon-gamma; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000072105:Superoxide Dismutase-1; D005980:Glutathione Reductase; D010751:Phosphopyruvate Hydratase; D003849:Deoxyguanosine
keywords: 8-OHdG; Toxoplasma gondii; neuron specific enolase; neuropathology; oxidative stress