title: 5,6-Dehydrokawain from Alpinia zerumbet promotes osteoblastic MC3T3-E1 cell differentiation.
abstract: Bone homeostasis is maintained by balancing bone formation and bone resorption, but an imbalance between them is associated with various bone-related diseases such as osteoporosis and rheumatoid arthritis. We found that 5,6-dehydrokawain (DK) and dihydro-5,6-dehydrokawain (DDK), which were isolated as promising compounds from Alpinia zerumbet rhizomes, promote differentiation of osteoblastic MC3T3-E1 cells. DK and DDK increased the alkaline phosphatase activity and matrix mineralization of MC3T3-E1 cells. DK exerts larger effects than DDK. The gene expression of runt-related transcription factor 2 and osterix, which are essential transcription factors in the early period of osteoblast differentiation, was significantly increased by DK treatment. The mRNA level of distal-less homeobox 5 was also enhanced by DK treatment, and DK activated the p38 mitogen-activated protein kinase pathway. Therefore, DK may have clinical potential for preventing osteoporosis, and could be considered as a potential anabolic therapeutic agent.
authors.affiliation: a Japan Food Research Laboratories , Osaka , Japan.|b Department of Chemistry, Graduate School of Science , Osaka City University , Osaka , Japan.
authors.forename: Momochika
authors.initials: M
authors.lastname: Kumagai
authors.affiliation: a Japan Food Research Laboratories , Osaka , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Mishima
authors.affiliation: a Japan Food Research Laboratories , Osaka , Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Watanabe
authors.affiliation: a Japan Food Research Laboratories , Osaka , Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Harada
authors.affiliation: a Japan Food Research Laboratories , Osaka , Japan.
authors.forename: Izumi
authors.initials: I
authors.lastname: Yoshida
authors.affiliation: a Japan Food Research Laboratories , Osaka , Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fujita
authors.affiliation: a Japan Food Research Laboratories , Osaka , Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Watai
authors.affiliation: c Faculty of Agriculture, Department of Bioscience and Biotechnology , University of the Ryukyus , Okinawa , Japan.
authors.forename: Shinkichi
authors.initials: S
authors.lastname: Tawata
authors.affiliation: b Department of Chemistry, Graduate School of Science , Osaka City University , Osaka , Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nishikawa
authors.affiliation: b Department of Chemistry, Graduate School of Science , Osaka City University , Osaka , Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D032429:Alpinia / Q000737:chemistry*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D050659:Core Binding Factor Alpha 1 Subunit / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects* / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry; D011753:Pyrones / Q000302:isolation & purification / Q000494:pharmacology*; D012333:RNA, Messenger / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry; D000076182:Sp7 Transcription Factor; D014157:Transcription Factors / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C501120:Dlx5 protein, mouse; D018398:Homeodomain Proteins; D010936:Plant Extracts; D011753:Pyrones; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D014157:Transcription Factors; C052297:5,6-dehydrokawain; C052296:7,8-dihydro-5,6-dehydrokawain; D048051:p38 Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase
keywords: 5,6-dehydrokawain; Alpinia zerumbet; MC3T3-E1; osteoblast; osteoporosis