title: Multilocus microsatellite analysis of European and African Candida glabrata isolates.
abstract: This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Chillemi
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Lo Passo
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
authors.forename: A D
authors.initials: AD
authors.lastname: van Diepeningen
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Rharmitt
authors.affiliation: Department of Human Pathology, University of Messina, Via Consolare Valeria, 98125, Messina, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Delfino
authors.affiliation: Department of Human Pathology, University of Messina, Via Consolare Valeria, 98125, Messina, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cascio
authors.affiliation: Department of Microbiology, Plateau State University, P.M.B 2012, Bokkos, Plateau State, Nigeria.
authors.forename: N E
authors.initials: NE
authors.lastname: Nnadi
authors.affiliation: Medical Microbiology Department, Faculty of Medicine, Uludag University, 16059, Bursa, Turkey.
authors.forename: B D
authors.initials: BD
authors.lastname: Cilo
authors.affiliation: Biology Department, Minho University, Campus de Gualtar, 4710-057, Braga, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Sampaio
authors.affiliation: Institut für Pilzkrankheiten, Luisenstrasse 50, 10117, Berlin, Germany.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Tietz
authors.affiliation: Servicio de Microbiología, Hospital Universitario y Politécnico La Fe, Valencia, España.
authors.forename: J
authors.initials: J
authors.lastname: Pemán
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Criseo
authors.affiliation: Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy. oromeo@unime.it.|IRCCS Centro Neurolesi "Bonino-Pulejo", SS113, 98124, Messina, Italy. oromeo@unime.it.
authors.forename: O
authors.initials: O
authors.lastname: Romeo
authors.affiliation: IRCCS Centro Neurolesi "Bonino-Pulejo", SS113, 98124, Messina, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Scordino
mesh_terms: D000349:Africa; D000483:Alleles; D041221:Candida glabrata / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D004271:DNA, Fungal; D004783:Environmental Microbiology; D005060:Europe; D056426:Genetic Loci; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D018895:Microsatellite Repeats; D058885:Multilocus Sequence Typing
chemical_list: D004271:DNA, Fungal