title: Asymmetrical Deterministic Lateral Displacement Gaps for Dual Functions of Enhanced Separation and Throughput of Red Blood Cells.
abstract: Deterministic lateral displacement (DLD) method for particle separation in microfluidic devices has been extensively used for particle separation in recent years due to its high resolution and robust separation. DLD has shown versatility for a wide spectrum of applications for sorting of micro particles such as parasites, blood cells to bacteria and DNA. DLD model is designed for spherical particles and efficient separation of blood cells is challenging due to non-uniform shape and size. Moreover, separation in sub-micron regime requires the gap size of DLD systems to be reduced which exponentially increases the device resistance, resulting in greatly reduced throughput. This paper shows how simple application of asymmetrical DLD gap-size by changing the ratio of lateral-gap (GL) to downstream-gap (GD) enables efficient separation of RBCs without greatly restricting throughput. This method reduces the need for challenging fabrication of DLD pillars and provides new insight to the current DLD model. The separation shows an increase in DLD critical diameter resolution (separate smaller particles) and increase selectivity for non-spherical RBCs. The RBCs separate better as compared to standard DLD model with symmetrical gap sizes. This method can be applied to separate non-spherical bacteria or sub-micron particles to enhance throughput and DLD resolution.
authors.affiliation: Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Block E4 #04-08, 117583, Singapore.|Cellular and Molecular Bioengineering Lab, National University of Singapore, Block E3A, #07-06, 7 Engineering Drive 1, 117574, Singapore.|Singapore Institute for Neurotechnology (SINAPSE), 28 Medical Dr. #05-COR, 117456, Singapore.
authors.forename: Kerwin Kwek
authors.initials: KK
authors.lastname: Zeming
authors.affiliation: Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Block E4 #04-08, 117583, Singapore.|Cellular and Molecular Bioengineering Lab, National University of Singapore, Block E3A, #07-06, 7 Engineering Drive 1, 117574, Singapore.|NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), National University of Singapore, 05-01 28 Medical Drive, Singapore 117456, Singapore.
authors.forename: Thoriq
authors.initials: T
authors.lastname: Salafi
authors.affiliation: Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Block E4 #04-08, 117583, Singapore.|Singapore Institute for Neurotechnology (SINAPSE), 28 Medical Dr. #05-COR, 117456, Singapore.
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Block E4 #04-08, 117583, Singapore.|Cellular and Molecular Bioengineering Lab, National University of Singapore, Block E3A, #07-06, 7 Engineering Drive 1, 117574, Singapore.|NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), National University of Singapore, 05-01 28 Medical Drive, Singapore 117456, Singapore.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002469:Cell Separation / Q000379:methods*; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D046210:Microfluidic Analytical Techniques; D010316:Particle Size