title: Data in support of proteomic analysis of pneumococcal pediatric clinical isolates to construct a protein array.
abstract: Surface proteins play key roles in the interaction between cells and their environment, and in pathogenic microorganisms they are the best targets for drug or vaccine discovery and/or development. In addition, surface proteins can be the basis for serodiagnostic tools aiming at developing more affordable techniques for early diagnosis of infection in patients. We carried out a proteomic analysis of a collection of pediatric clinical isolates of Streptococcus pneumoniae, an important human pathogen responsible for more than 1.5 million child deaths worldwide. For that, cultured live bacterial cells were "shaved" with trypsin, and the recovered peptides were analyzed by LC/MS/MS. We selected 95 proteins to be produced as recombinant polypeptides, and printed them on an array. We probed the protein array with a collection of patient sera to define serodiagnostic antigens. The mass spectrometry proteomics data correspond to those published in [1] and have been deposited to the ProteomeXchange Consortium [2] via the PRIDE partner repository [3] with the dataset identifier PXD001740. The protein array raw data are provided as supplemental material in this article.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Olaya-Abril
authors.affiliation: Sección de Enfermedades Infecciosas Pe diátricas e Inmunopatología, Hospital Universitario Infantil Virgen del Rocío, Sevilla, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Obando
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, Córdoba, Spain.
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Rodríguez-Ortega
keywords: Diagnostics; Pneumococcus; Protein arrays; Proteomics