title: Genome-wide identification, characterization and expression analysis of calmodulin-like (CML) proteins in tomato (Solanum lycopersicum).
abstract: Calcium (Ca(2+)) has emerged as a significant secondary messenger that regulates the activities of hormonal and environmental signals that are associated with biotic and abiotic stresses. Ca(2+) binding proteins typically contain a Ca(2+) binding EF-hand (a helix-loop-helix structure) motif. In this study, tomato genes encoding calmodulin-like (CML) proteins that possess EF-hand motifs and no other identifiable functional domains were analyzed. Using genome analysis and BLAST searches in database, 52 CML genes were identified in tomato. Comprehensive analyses, including evolutionary relationships, gene structures, chromosomal locations, functional annotations, and gene duplications, were performed. Distribution mapping exhibited that 52 SlCML proteins containing different intron/exon patterns were unevenly distributed among ten chromosomes. In addition, 24 SlCML proteins were predicted as segmentally duplicated. Conserved motifs, promoter cis-regulatory elements, organ-based expression patterns and expression analyses indicated the potential responsiveness of SlCML proteins to abiotic stresses and phytohormones. These results illustrate the complexity of the CML gene family and indicate a potential vital role for these molecules in tomato growth and development as Ca(2+) signal transducers.
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shoaib
authors.initials: S
authors.lastname: Munir
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Muhammad Rehman Gul
authors.initials: MR
authors.lastname: Khan
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Song
authors.affiliation: National R&D Branch Center for Conventional Freshwater Fish Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Sadia
authors.initials: S
authors.lastname: Munir
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Zhibiao
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: ttwang@mail.hzau.edu.cn.
authors.forename: Taotao
authors.initials: T
authors.lastname: Wang
mesh_terms: D002147:Calmodulin / Q000096:biosynthesis / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D030561:Databases, Nucleic Acid; D020440:Gene Duplication; D005786:Gene Expression Regulation / Q000502:physiology*; D055106:Genome-Wide Association Study; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D002147:Calmodulin; D010940:Plant Proteins
keywords: Abiotic stress; Calmodulin-like proteins (CML); Expression pattern; Plant hormones; Tomato