title: Association of ADAMTS-7 Levels with Cardiac Function in a Rat Model of Acute Myocardial Infarction.
abstract: OBJECTIVE
  High ADAMTS-7 levels are associated with acute myocardial infarction (AMI), although its involvement in ventricular remodeling is unclear. In this study, we investigated the association between ADAMTS-7 expression and cardiac function in a rat AMI model.
  
  
  METHODS
  Sprague-Dawley rats were randomized into AMI (n = 40) and sham (n = 20) groups. The left anterior descending artery was sutured to model AMI. Before surgery and 7, 14, 28, and 42 days post-surgery, ADAMTS-7 and brain natriuretic peptide (BNP), and cartilage oligomeric matrix protein (COMP) were assessed by ELISA, western blot, real-time RT-PCR, and/or immunohistochemistry. Cardiac functional and structural parameters were assessed by M-mode echocardiography.
  
  
  RESULTS
  After AMI, plasma ADAMTS-7 levels increased, peaking on day 28 (AMI: 13.2 ± 6.3 vs. sham: 3.4 ± 1.3 ng/ml, P < 0.05). Compared with the sham group, ADAMTS-7 expression was higher in the infarct zone at day 28. COMP present in normal myocardium was degraded by day 28 post-AMI. Plasma ADAMTS-7 correlated positively with BNP (r = 0.642, P = 0.025), left ventricular end-diastolic diameter (r = 0.695, P = 0.041), left ventricular end-systolic diameter (r = 0.710, P = 0.039), left ventricular ejection fraction (r = 0.695, P = 0.036), and left ventricular short-axis fractional shortening (r = 0.721, P = 0.024).
  
  
  CONCLUSIONS
  ADAMTS-7 levels may reflect the degree of ventricular remodeling after AMI.
authors.affiliation: Department of Cardiology, China-Japan Friendship Hospital, Beijing, Beijing, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Changan
authors.initials: C
authors.lastname: Yu
authors.forename: Jiahui
authors.initials: J
authors.lastname: Li
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Gao
authors.forename: Yuannan
authors.initials: Y
authors.lastname: Ke
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jingang
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000071118:ADAMTS7 Protein / Q000097:blood* / Q000235:genetics*; D000818:Animals; D064236:Cartilage Oligomeric Matrix Protein / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004452:Echocardiography; D008297:Male; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D020097:Natriuretic Peptide, Brain / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
chemical_list: C577538:COMP protein, rat; D064236:Cartilage Oligomeric Matrix Protein; C495963:natriuretic peptide precursor type B, rat; D020097:Natriuretic Peptide, Brain; D000071118:ADAMTS7 Protein; C539627:Adamts7 protein, rat