title: Drosophila Cyclin G Is a Regulator of the Notch Signalling Pathway during Wing Development.
abstract: Notch signalling regulates a multitude of differentiation processes during Drosophila development. For example, Notch activity is required for proper wing vein differentiation which is hampered in mutants of either the receptor Notch, the ligand Delta or the antagonist Hairless. Moreover, the Notch pathway is involved in several aspects of Drosophila oogenesis as well. We have identified Drosophila Cyclin G (CycG) as a molecular interaction partner of Hairless, the major antagonist in the Notch signalling pathway, in vitro and in vivo. Loss of CycG was shown before to cause female sterility and to disturb the architecture of the egg shell. Nevertheless, Notch dependent processes during oogenesis appeared largely unaffected in cycG mutant egg chambers. Loss of CycG modified the dominant wing phenotypes of Notch, Delta and Hairless mutants. Whereas the Notch loss of function phenotype was ameliorated by a loss of CycG, the phenotypes of either Notch gain of function or of Delta or Hairless loss of function were enhanced. In contrast, loss of CycG had only a minor effect on the wing vein phenotype of mutants affecting the EGFR signalling pathway emphasizing the specificity of the interaction of CycG and Notch pathway members.
authors.affiliation: Institut für Genetik, Universität Hohenheim, Garbenstr. 30, 70599 Stuttgart, Germany.
authors.forename: Anja C
authors.initials: AC
authors.lastname: Nagel
authors.affiliation: Institut für Genetik, Universität Hohenheim, Garbenstr. 30, 70599 Stuttgart, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Szawinski
authors.affiliation: Institut für Genetik, Universität Hohenheim, Garbenstr. 30, 70599 Stuttgart, Germany.
authors.forename: Mirjam
authors.initials: M
authors.lastname: Zimmermann
authors.affiliation: Institut für Genetik, Universität Hohenheim, Garbenstr. 30, 70599 Stuttgart, Germany.
authors.forename: Anette
authors.initials: A
authors.lastname: Preiss
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019521:Body Patterning / Q000235:genetics; D056746:Cyclin G / Q000378:metabolism*; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*; D014921:Wings, Animal / Q000196:embryology* / Q000378:metabolism
chemical_list: D056746:Cyclin G; D029721:Drosophila Proteins; D051880:Receptors, Notch