title: The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice.
abstract: Kurozu is a traditional Japanese rice vinegar. During fermentation and aging of the Kurozu liquid in an earthenware jar over 1 year, a solid residue called Kurozu Moromi is produced. In the present study, we evaluated whether concentrated Kurozu or Kurozu Moromi could ameliorate cognitive dysfunction in the senescence-accelerated P8 mouse. Senescence-accelerated P8 mice were fed 0.25% (w/w) concentrated Kurozu or 0.5% (w/w) Kurozu Moromi for 4 or 25 weeks. Kurozu suppressed cognitive dysfunction and amyloid accumulation in the brain, while Kurozu Moromi showed a tendency to ameliorate cognitive dysfunction, but the effect was not significant. We hypothesize that concentrated Kurozu has an antioxidant effect; however, the level of lipid peroxidation in the brain did not differ in senescence-accelerated P8 mice. DNA microarray analysis indicated that concentrated Kurozu increased HSPA1A mRNA expression, a protein that prevents protein misfolding and aggregation. The increase in HSPA1A expression by Kurozu was confirmed using quantitative real-time PCR and immunoblotting methods. The suppression of amyloid accumulation by concentrated Kurozu may be associated with HSPA1A induction. However, concentrated Kurozu could not increase HSPA1A expression in mouse primary neurons, suggesting it may not directly affect neurons.
authors.affiliation: Department of Veterinary Pathobiology, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kanouchi
authors.affiliation: Department of Veterinary Pathobiology, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kakimoto
authors.affiliation: Department of Veterinary Pathobiology, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Hideya
authors.initials: H
authors.lastname: Nakano
authors.affiliation: Department of Veterinary Pathobiology, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Institute for Food Sciences, Hirosaki University, Yanagawa, Aomori, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakai
authors.affiliation: Faculty of Fisheries, Kagoshima University, Shimoarata, Kagoshima, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Shiozaki
authors.affiliation: Department of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Akikoka
authors.affiliation: Veterinary Clinical Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Konosuke
authors.initials: K
authors.lastname: Otomaru
authors.affiliation: Sakamoto Kurozu, Inc., Uenosono-cho, Kagoshima, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Nagano
authors.affiliation: Department of Veterinary Anatomy, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima, Japan.
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D000375:Aging / Q000473:pathology; D000682:Amyloid / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003072:Cognition Disorders / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology*; D005247:Feeding Behavior / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000682:Amyloid; D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; C498550:Hsp70.3 protein, mouse; D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D019342:Acetic Acid