title: [Effect of Wenyang Decoction on the Differentiation of CD34+ Progenitor Cells in Occupational Asthma Model Rats].
abstract: OBJECTIVE
  To study the effect of Wenyang Decoction (WD) on the differentiation of CD34+ progenitor cells of occupational asthma (OA) model rats.
  
  
  METHODS
  Fifty healthy male SD rats were randomly divided into five groups, i.e., the model group, the blank control group,the WD group,the Western medicine group,the combined group, 10 in each group. Prednisone suspension (10 mg/kg) was administered to rats in the Western medicine group by gastrogavage. WD (20 g/kg) was administered to rats in the WD group by gastrogavage. Prednisone suspension plus WD was administered to rats in the combined group by gastrogavage. Normal saline was administered to rats in the model group and the blank control group by gastrogavage. The general condition of rats was observed. Expression levels of peripheral blood IL-5 and eotaxin, eosinophils (EOS), CD34+, CC chemokine receptor 3 (CCR3+) in bone marrow suspension were detected by ELISA, Wirght-Giemsa, and flow cytometry, respectively.
  
  
  RESULTS
  Compared with the blank control group,expression levels of IL-5 and eotaxin in peripheral blood were significantly higher (P < 0.01), and the count of EOS and CD34+ cells, as well as CD34+ /CCR3+ significantly increased (P < 0.01) in the model group. Compared with the model group, expression levels of IL-5 and eotaxin, the count of EOS, CD34+ cells, CD34+ / CCR3+ were lowered in three treated groups (P < 0.01). Compared with the Western medicine group, the count of EOS and CD34+ / CCR3+ decreased in the combined group (P < 0.01). The count of EOS was significantly lower in the combined group than in the WD group (P < 0.01).
  
  
  CONCLUSIONS
  WD could reduce levels of in vivo inflammatory factors, and restrain the differentiation and recruitment of EOS,thereby alleviating the differentiation of CD34 progenitor cells to EOS.
authors.forename: Yang-min
authors.initials: YM
authors.lastname: Jia
authors.forename: Zu-ying
authors.initials: ZY
authors.lastname: Hu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Shu-juan
authors.initials: SJ
authors.lastname: Wang
authors.forename: Jing-yin
authors.initials: JY
authors.lastname: Han
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Yan
mesh_terms: D000818:Animals; D018952:Antigens, CD34; D059366:Asthma, Occupational / Q000188:drug therapy*; D001853:Bone Marrow; D002454:Cell Differentiation; D054413:Chemokine CCL11; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004804:Eosinophils; D005434:Flow Cytometry; D015848:Interleukin-5; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D054397:Receptors, CCR3; D013234:Stem Cells
chemical_list: D018952:Antigens, CD34; C515456:CCL11 protein, human; D054413:Chemokine CCL11; D004365:Drugs, Chinese Herbal; D015848:Interleukin-5; D054397:Receptors, CCR3