title: The banana E2 gene family: Genomic identification, characterization, expression profiling analysis.
abstract: The E2 is at the center of a cascade of Ub1 transfers, and it links activation of the Ub1 by E1 to its eventual E3-catalyzed attachment to substrate. Although the genome-wide analysis of this family has been performed in some species, little is known about analysis of E2 genes in banana. In this study, 74 E2 genes of banana were identified and phylogenetically clustered into thirteen subgroups. The predicted banana E2 genes were distributed across all 11 chromosomes at different densities. Additionally, the E2 domain, gene structure and motif compositions were analyzed. The expression of all of the banana E2 genes was analyzed in the root, stem, leaf, flower organs, five stages of fruit development and under abiotic stresses. All of the banana E2 genes, with the exception of few genes in each group, were expressed in at least one of the organs and fruit developments, which indicated that the E2 genes might involve in various aspects of the physiological and developmental processes of the banana. Quantitative RT-PCR (qRT-PCR) analysis identified that 45 E2s under drought and 33 E2s under salt were induced. To the best of our knowledge, this report describes the first genome-wide analysis of the banana E2 gene family, and the results should provide valuable information for understanding the classification, cloning and putative functions of this family.
authors.affiliation: Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Dong
authors.affiliation: Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China.
authors.forename: Huigang
authors.initials: H
authors.lastname: Hu
authors.affiliation: Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China.
authors.forename: Dengwei
authors.initials: D
authors.lastname: Jue
authors.affiliation: Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China.
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China.
authors.forename: Jianghui
authors.initials: J
authors.lastname: Xie
authors.affiliation: Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang 524091, China. Electronic address: liqiangj@zju.edu.cn.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Jia
mesh_terms: D000595:Amino Acid Sequence; D032461:Chromosomes, Plant / Q000235:genetics; D005091:Exons / Q000235:genetics; D005638:Fruit / Q000235:genetics / Q000254:growth & development; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018745:Genome, Plant; D007438:Introns / Q000235:genetics; D005810:Multigene Family; D028521:Musa / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000072417:Protein Domains; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins
keywords: Abiotic stress; Banana; Expression analysis; Gene family; Genome wide analysis; Ubiquitin-conjugating enzyme