title: L-arginine, an active component of salmon milt nucleoprotein, promotes thermotolerance via Sirtuin in Caenorhabditis elegans.
abstract: We previously showed that salmon milt nucleoprotein (NP) promotes thermotolerance in Caenorhabditis elegans; however, the active component and physiological mechanism of this effect has remained unclear. l-arginine (AR) is a major component of protamine and thus it has been proposed as the possible active component of NP. In this study, the viability of C. elegans treated with AR under heat stress was assessed and AR was shown to extend the survival term of the heat-stressed organisms. Additionally, AR was shown to restore the thrashing movement of the worms that is suppressed by heat stress. Treatment with AR was furthermore shown to promote thermotolerance in a DAF-16- and SIR-2.1-dependent manner, where DAF-16 and SIR-2.1 are homologs of FoxO and SirT1, respectively. Taken together, these data suggest that AR is one of the active components of NP and promotes thermotolerance via the activation of DAF-16 and SIR-2.1.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Furuhashi
authors.affiliation: Gene Trophology Institute Co., 3-1-3 Megumino Kita, Eniwa, Hokkaido 061-1374, Japan.
authors.forename: Masaji
authors.initials: M
authors.lastname: Matsunaga
authors.affiliation: Nissan Chemical Industries, Co., Ltd., 3-7-1 Kanda-Nishikicho, Chiyoda, Tokyo 101-0054, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Asahara
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address: sakamoto@biol.tsukuba.ac.jp.
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Sakamoto
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D049628:Body Size / Q000187:drug effects; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D029941:Fish Proteins / Q000737:chemistry* / Q000494:pharmacology; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D009698:Nucleoproteins / Q000737:chemistry* / Q000494:pharmacology; D011479:Protamines / Q000737:chemistry / Q000494:pharmacology; D012474:Salmon; D037761:Sirtuins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D029941:Fish Proteins; D051858:Forkhead Transcription Factors; D009698:Nucleoproteins; D011479:Protamines; C108831:daf-16 protein, C elegans; D001120:Arginine; C507014:SIR-2.1 protein, C elegans; D037761:Sirtuins
keywords: Caenorhabditis elegans; DAF-16; Nucleoprotein; SIR-2.1; Thermotolerance; l-arginine