title: The Formulation of Bacteriophage in a Semi Solid Preparation for Control of Propionibacterium acnes Growth.
abstract: OBJECTIVE
  To isolate and characterise phage which could lyse P. acnes and to formulate the phage into a delivery form for potential application in topical treatment of acne infection.
  
  
  RESULTS
  Using standard phage isolation techniques, ten phage capable of lysing P. acnes were isolated from human skin microflora. Their genomes showed high homology to previously reported P. acnes phage. These phage were formulated into cetomacrogol cream aqueous at a concentration of 2.5x108 PFU per gram, and shown to lyse underlying P. acnes cells grown as lawn cultures. These phage formulations remained active for at least 90 days when stored at four degrees Celsius in a light protected container.
  
  
  CONCLUSIONS
  P. acnes phage formulated into cetomacrogol cream aqueous will lyse surrounding and underlying P. acnes bacteria, and are effective for at least 90 days if stored appropriately.
  
  
  CONCLUSIONS
  There are few reports of phage formulation into semi solid preparations for application as phage therapy. The formulation method described here could potentially be applied topically to treat human acne infections. The potential exists for this model to be extended to other phage applied to treat other bacterial skin infections.
authors.affiliation: La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, Australia.
authors.forename: Teagan L
authors.initials: TL
authors.lastname: Brown
authors.affiliation: Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC, Australia.
authors.forename: Steve
authors.initials: S
authors.lastname: Petrovski
authors.affiliation: La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, Australia.
authors.forename: Zoe A
authors.initials: ZA
authors.lastname: Dyson
authors.affiliation: Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: Seviour
authors.affiliation: La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, Australia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Tucci
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002626:Chemistry, Pharmaceutical; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011425:Propionibacterium acnes / Q000254:growth & development* / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology
chemical_list: D004279:DNA, Viral