title: Antibacterial and cytotoxic triterpenoids from the roots of Combretum racemosum.
abstract: A new pentacyclic triterpenoid glucoside, together with fourteen known compounds, was isolated from the roots of Combretum racemosum. Combretaceae). The structure of the new compound was established as 28-O-β-d-glucopyranosyl-2α,3β,21β,23-tetrahydroxyolean-18-en-28-oate (1) on the basis of detailed spectroscopic data including MS, 1D, and 2D NMR. The inhibitory activity of compounds 1-15 against promyelocytic leukemia HL-60 and human erythromyeloblastoid leukemia K562 cell lines was evaluated. Compounds 11 (3-O-β-acetyl-ursolic acid), 14 (betulinic acid), and 15 (quadranoside II) exhibited significant cytotoxicity, with IC50 values of 13 to 50 μM. Among the isolated triterpenes, compounds 1, 3 (arjungenin), 5 (terminolic acid), and 11 exhibited moderate antibacterial activity against Staphylococcus aureus, Escherichia coli and Enterococcus faecalis (MICs within a range of 64 and 256 μg/mL).
authors.affiliation: ICMR-UMR CNRS 7312, Groupe Isolement et Structure, Campus Sciences, Bât. 18, BP 1039, 51687 Reims Cedex 2, France; Laboratoire de Chimie Organique et de Substances Naturelles, 22 BP 582 Abidjan 22, Université Félix-Houphouët Boigny d'Abidjan-Cocody, Ivory Coast, France.
authors.forename: Diane Patricia Apie
authors.initials: DP
authors.lastname: Gossan
authors.affiliation: ICMR-UMR CNRS 7312, Groupe Isolement et Structure, Campus Sciences, Bât. 18, BP 1039, 51687 Reims Cedex 2, France. Electronic address: abdulmagid.alabdulmagid@univ-reims.fr.
authors.forename: Abdulmagid
authors.initials: A
authors.lastname: Alabdul Magid
authors.affiliation: Laboratoire de Chimie Organique et de Substances Naturelles, 22 BP 582 Abidjan 22, Université Félix-Houphouët Boigny d'Abidjan-Cocody, Ivory Coast, France.
authors.forename: Philomène Akoua
authors.initials: PA
authors.lastname: Yao-Kouassi
authors.affiliation: Laboratoire de microbiologie, EA 4691, « Biomatériaux et inflammation en site osseux », UFR de pharmacie, 1, rue du Maréchal-Juin, 51096 Reims Cedex, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Josse
authors.affiliation: Laboratoire de microbiologie, EA 4691, « Biomatériaux et inflammation en site osseux », UFR de pharmacie, 1, rue du Maréchal-Juin, 51096 Reims Cedex, France.
authors.forename: Sophie C
authors.initials: SC
authors.lastname: Gangloff
authors.affiliation: MEDyC UMR CNRS 7369, URCA, Faculté de Pharmacie, SFR CAP Santé, Reims, France.
authors.forename: Hamid
authors.initials: H
authors.lastname: Morjani
authors.affiliation: ICMR-UMR CNRS 7312, Groupe Isolement et Structure, Campus Sciences, Bât. 18, BP 1039, 51687 Reims Cedex 2, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Voutquenne-Nazabadioko
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D029765:Combretum / Q000737:chemistry*; D007560:Cote d'Ivoire; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C000610371:28-O-beta-d-glucopyranosyl-2alpha,3beta,21beta,23-tetrahydroxyolean-18-en-28-oate; D000900:Anti-Bacterial Agents; D005960:Glucosides; D014315:Triterpenes
keywords: Antibacterial activity; Combretaceae; Combretum racemosum; Cytotoxic activity; Triterpenes