title: Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF‑7 cells by reducing FGF4 protein expression.
abstract: The present study aimed to investigate the effects of bone marrow‑derived mesenchymal stem cells (BMSCs) that had been pretreated with pioglitazone and/or rosiglitazone on the growth and proliferation rate of MCF‑7 cells. The adhesive interaction between the BMSCs and the MCF‑7 cancer cells revealed that the pretreatment of BMSCs with a combination of two types of thiazolidinedione drug reduced the growth and proliferation rate of the MCF‑7 cells. The proliferation rate of the MCF‑7 cells could also be reduced by the non‑adhesive interaction of the cancer cells with BMSCs pretreated with pioglitazone and/or rosiglitazone. The growth and proliferation rate reduction effects on the MCF‑7 cells may be attributed to the reduction in the protein level of fibroblast growth factor 4 (FGF4) in the conditioned medium of the pretreated BMSCs. The evidence that the low protein level of FGF4 in the conditioned medium of the pretreated BMSCs perturbed the proliferation rate of the MCF‑7 cells by reducing the levels of Ki‑67 and proliferating cell nuclear antigen transcripts in the cancer cells was also demonstrated in the present study using a FGF4‑neutralizing antibody. All the above findings demonstrate that future studies on the correlation between FGF4 and pretreated BMSCs would be beneficial.
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia.
authors.forename: Boon-Yin
authors.initials: BY
authors.lastname: Khoo
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia.
authors.forename: Kalpanah
authors.initials: K
authors.lastname: Nadarajan
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia.
authors.forename: Siang-Yian
authors.initials: SY
authors.lastname: Shim
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia.
authors.forename: Noorizan
authors.initials: N
authors.lastname: Miswan
authors.affiliation: Division of Oncology and Haematology, Charité Campus Mitte, Humboldt University of Berlin, D‑10099 Berlin, Germany.
authors.forename: Chuan-Bing
authors.initials: CB
authors.lastname: Zang
authors.affiliation: Division of Oncology and Haematology, Charité Campus Mitte, Humboldt University of Berlin, D‑10099 Berlin, Germany.
authors.forename: Kurt
authors.initials: K
authors.lastname: Possinger
authors.affiliation: Division of Oncology and Haematology, Charité Campus Mitte, Humboldt University of Berlin, D‑10099 Berlin, Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Elstner
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D051519:Fibroblast Growth Factor 4 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D061986:MCF-7 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000077205:Pioglitazone; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D018925:Chemokines; D017077:Culture Media, Conditioned; D016207:Cytokines; C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D045162:Thiazolidinediones; D000077154:Rosiglitazone; C089946:2,4-thiazolidinedione; D000077205:Pioglitazone