title: Endogenous CBS-H2S Pathway Contributes to the Development of CCI-Induced Neuropathic Pain.
abstract: Studies showed a complex relationship between hydrogen sulfide (H2S) and neuropathic pain. In this study, the relationship between endogenous CBS-H2S pathway in L4-6 spinal cord and neuropathic pain was explored. A total of 163 adult Kunming mice were used in this study. CBS expression and H2S formation in L4-6 spinal cord were detected in the development of neuropathic pain firstly. Then, effect of AOAA, an CBS inhibitor, on treatment of neuropathic pain by chronic construction injury surgery (CCI) was detected. Pain thresholds and activation of NF-κB(p65), ERK1/2 and CREB were measured as biomarks of neuropathic pain. Results showed that CCI surgery significantly upregulated protein expression of CBS and H2S formation. Correlation analysis showed pain thresholds had negative relationships with protein expression of CBS and H2S formation. Treatment with AOAA, a CBS inhibitor, inhibited CCI-induced upregulation of CBS expression and H2S formation (P < 0.05). Further, AOAA significantly decreased activation of NF-κB(p65), ERK1/2 and CREB pathway, and reversed CCI-induced allodynia (P < 0.05). This indicated that CBS-H2S pathway promoted the development of neuropathic pain. CBS-H2S pathway could be a promising target for treatment of neuropathic pain.
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gui
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China. 502789123@qq.com.
authors.forename: Aiyuan
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China.
authors.forename: Bihui
authors.initials: B
authors.lastname: Qiu
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China.
authors.forename: Daming
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, The Maternal and Child Health Hospital of Hunan Province, Changsha, 410008, Hunan Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Radiology, The First Affiliated Hospital of Nanhua University, Hengyang, 421001, Hunan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D000818:Animals; D003251:Constriction, Pathologic; D003541:Cystathionine beta-Synthase / Q000096:biosynthesis*; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008159:Lumbar Vertebrae; D008297:Male; D051379:Mice; D009437:Neuralgia / Q000378:metabolism* / Q000473:pathology; D020426:Sciatic Neuropathy / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D003541:Cystathionine beta-Synthase; D006862:Hydrogen Sulfide
keywords: Chronic sciatic nerve construction injury (CCI); Cystathionine β-syntheses (CBS); Hydrogen sulfide (H2S); O-(carboxymethyl) hydroxylamine hemihydrochloride (AOAA)