title: Antimyeloperoxidase antibody is a biomarker for progression of diabetic retinopathy.
abstract: OBJECTIVE
  To study the correlation between serum antimyeloperoxidase (MPO) antibody levels with severity of diabetic retinopathy (DR).
  
  
  METHODS
  Study subjects included 60 consecutive cases of type 2 diabetes mellitus (DM): no diabetic retinopathy (NODR, n=20); nonproliferative DR (NPDR, n=20); proliferative DR (PDR, n=20) and 20 healthy controls. Best corrected visual acuity (BCVA) was measured on logMAR scale. Serum anti-MPO antibody levels were evaluated using ELISA IgG kit. Serum urea and creatinine was measured using standard protocol. Data were analysed statistically.
  
  
  RESULTS
  Mean serum anti-MPO antibody (RU/ml) was 16.94 ± 4.85 in controls, 17.66 ± 4.78 in NODR, 21.51 ± 5.27 in NPDR and 37.27 ± 11.92 in PDR groups. On ANOVA, significant difference in visual acuity was found among the study groups (F=73.46, p<0.001). Serum anti-MPO antibody was correlated significantly with decrease in visual acuity (F=48.40, p<0.001), increase in serum urea (F=128.13, p<0.001) and creatinine (F=77.10, p<0.001).
  
  
  CONCLUSIONS
  Increase in serum anti-MPO antibody levels correlate with increased severity of DR. Serum anti-MPO antibody may be a noteworthy biochemical marker for progression of retinopathy from nonproliferative to proliferative stage.
authors.affiliation: Retina service, Department of Ophthalmology, King George's Medical University, Lucknow, India.
authors.forename: Shivani
authors.initials: S
authors.lastname: Sinha
authors.affiliation: Retina service, Department of Ophthalmology, King George's Medical University, Lucknow, India. Electronic address: sandeepsaxena2020@yahoo.com.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Department of Rheumatology, King George's Medical University, Lucknow, India.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Das
authors.affiliation: Department of Preventive and Social Medicine, King George's Medical University, Lucknow, India.
authors.forename: Senthamizh
authors.initials: S
authors.lastname: Prasad
authors.affiliation: Retina service, Department of Ophthalmology, King George's Medical University, Lucknow, India.
authors.forename: Shashi Kumar
authors.initials: SK
authors.lastname: Bhasker
authors.affiliation: Department of Biochemistry, King George's Medical University, Lucknow, India.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Mahdi
authors.affiliation: Laboratory of Structural Biology and Proteomics, Central Laboratories, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. Electronic address: kruzliakpeter@gmail.com.
authors.forename: Peter
authors.initials: P
authors.lastname: Kruzliak
mesh_terms: D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000097:blood / Q000150:complications / Q000276:immunology / Q000503:physiopathology*; D015415:Biomarkers / Q000097:blood; D003404:Creatinine / Q000097:blood; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D003930:Diabetic Retinopathy / Q000097:blood / Q000276:immunology / Q000503:physiopathology*; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000037:antagonists & inhibitors*; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D062606:Tertiary Care Centers; D014508:Urea / Q000097:blood; D014792:Visual Acuity; D018630:Vitreoretinopathy, Proliferative / Q000097:blood / Q000150:complications / Q000209:etiology* / Q000276:immunology
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D003404:Creatinine; D006442:Glycated Hemoglobin; D009195:Peroxidase; D014508:Urea; C517652:hemoglobin A1c protein, human
keywords: Antimyeloperoxidase antibody; Diabetic retinopathy; Serum creatinine; Serum urea; Visual acuity