title: Effects of riluzole on P2X7R expression in the spinal cord in rat model of neuropathic pain.
abstract: Neuropathic pain is becoming an intractable health threat, with its profound effect on quality of life, thus posing a major challenge to clinical therapy. Despite the reported efficacy of riluzole in some pain models, the underlying mechanism remains largely unknown. The present study aimed to assess the effects of riluzole in a rat model of neuropathic pain induced by chronic constriction injury (CCI). Subsequent to model establishment, paw withdrawal latencies (PWLs) and the paw withdrawal mecha threshold (PWT) rapidly decreased, coupled with inhibited microglial activation and upregulated P2X7R expression in the spinal cord dorsal horn (SCDH). Following intraperitoneal administration of riluzole (4mg/kg) once daily for 5 consecutive days as from day 3 after surgery, the mechanical allodynia and thermal hyperalgesia in the hind limbs were significantly attenuated. In addition, riluzole downregulated P2X7R expression and inhibited microglial activation in SCDH. Our results indicated that riluzole is effective in alleviating neuropathic pain and inhibiting microglial activation, presumably via the downregulated P2X7R expression in SCDH.
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China; Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical College, Xuzhou 221004, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Laboratory of Clinical and Experimental Pathology, Xuzhou Medical College, Xuzhou 221004, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China; Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical College, Xuzhou 221004, China; Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China; Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical College, Xuzhou 221004, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China; Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical College, Xuzhou 221004, China.
authors.forename: An-Qi
authors.initials: AQ
authors.lastname: Ge
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China; Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical College, Xuzhou 221004, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China; Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application Technology, Xuzhou Medical College, Xuzhou 221004, China. Electronic address: benjaminmb@163.com.
authors.forename: Bei
authors.initials: B
authors.lastname: Miao
mesh_terms: D000818:Animals; D003251:Constriction, Pathologic / Q000150:complications; D006930:Hyperalgesia / Q000188:drug therapy / Q000503:physiopathology; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D009437:Neuralgia / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology* / Q000627:therapeutic use; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*; D019782:Riluzole / Q000494:pharmacology* / Q000627:therapeutic use; D012584:Sciatic Nerve / Q000293:injuries; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism; D066148:Spinal Cord Dorsal Horn / Q000187:drug effects / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D058920:Purinergic P2X Receptor Antagonists; D058486:Receptors, Purinergic P2X7; D019782:Riluzole
keywords: Microglia; Neuropathic pain; P2X7 receptor; Riluzole; Spinal cord