title: Human adipose-derived stem cells promote vascularization of collagen-based scaffolds transplanted into nude mice.
abstract: OBJECTIVE
  After in vivo implantation of cell-loaded devices, only the cells close to the capillaries can obtain nutrients to maintain their functions. It is known that factors secreted by stem cells, rather than stem cells themselves, are fundamental to guarantee new vascularization in the area of implant.
  
  
  METHODS
  To investigate this possibility, we have grafted mice with Bilayer and Flowable Integra(®) scaffolds, loaded or not with human adipose-derived stem cells.
  
  
  RESULTS
  Our results support the therapeutic potential of human adipose-derived stem cells to induce new vascular networks of engineered organs and tissues.
  
  
  CONCLUSIONS
  This finding suggests that our approach can help to form new vascular networks that allow sufficient vascularization of engineered organs and tissues in cases of difficult wound healing due to ischemic conditions.
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Cherubino
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Valdatta
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Balzaretti
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.
authors.forename: Igor
authors.initials: I
authors.lastname: Pellegatta
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Rossi
authors.affiliation: Dipartimento di Scienze Chirurgiche e Morfologiche, Università degli Studi dell'Insubria, Via Guicciardini 9, 21100 Varese, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Protasoni
authors.affiliation: Dipartimento di Scienze Chirurgiche e Morfologiche, Università degli Studi dell'Insubria, Via Guicciardini 9, 21100 Varese, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Tedeschi
authors.affiliation: Dipartimento di Scienze Chirurgiche e Morfologiche, Università degli Studi dell'Insubria, Via Guicciardini 9, 21100 Varese, Italy.
authors.forename: Roberto S
authors.initials: RS
authors.lastname: Accolla
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.|"The Protein Factory" Research Center, Politecnico di Milano, ICRM-CNR Milano & Università dell'Insubria, Via Mancinelli 7, Milano, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bernardini
authors.affiliation: Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Via Dunant 3, Varese, Italy.|"The Protein Factory" Research Center, Politecnico di Milano, ICRM-CNR Milano & Università dell'Insubria, Via Mancinelli 7, Milano, Italy.
authors.forename: Rosalba
authors.initials: R
authors.lastname: Gornati
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D018914:Cells, Immobilized; D003094:Collagen / Q000737:chemistry*; D005260:Female; D064593:Heterografts; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003094:Collagen
keywords: human adipose-derived stem cells; new vascularization; tissue engineering; ulcers; wound healing