title: Synthesis of the Hemoglobin-Conjugated Polymer Micelles by Thiol Michael Addition Reactions.
abstract: Amphiphilic triblock copolymers mPEG-b-PMAC-b-PCL are synthesized using methoxyl poly(ethylene glycol), cyclic carbonic ester monomer including acryloyl group, and ε-caprolactone. Copolymers are self-assembled into core-shell micelles in aqueous solution. Thiolated hemoglobin (Hb) is conjugated with micelles sufficiently through thiol Michael addition reaction to form hemoglobin nanoparticles (HbNs) with 200 nm in diameter. The conjugation of Hb onto the micelle surface is further confirmed by X-ray photoelectron spectroscopy. Feeding ratio of copolymer micelles to Hb at 1:3 would lead to the highest hemoglobin loading efficiency 36.7 wt%. The UV results demonstrate that the gas transporting capacity of HbNs is well remained after Hb is conjugated with polymeric micelles. Furthermore, the obtained HbNs have no obvious detrimental effects on blood components in vitro. This system may thus have great potential as one of the candidates to be developed as oxygen carriers and provide a reference for the modification of protein drugs.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Taihang
authors.initials: T
authors.lastname: Li
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.|University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Chemistry, SUNY Albany, Albany, NY, 12222, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000166:Acridines / Q000737:chemistry; D002208:Caproates / Q000737:chemistry; D016503:Drug Delivery Systems; D006454:Hemoglobins / Q000138:chemical synthesis / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: C403603:10-methyl-9-(phenoxycarbonyl)acridinium; D000166:Acridines; D002208:Caproates; D006454:Hemoglobins; D007783:Lactones; D008823:Micelles; C079129:hemoglobin polymer; D011092:Polyethylene Glycols; C121056:caprolactone; D010100:Oxygen
keywords: hemoglobin; oxygen carriers; protein delivery; thiol Michael addition